LOCUS       DAOQJH010000001        19246 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_1304_length_19246_cov_7.754312, whole genome shotgun sequence.
ACCESSION   DAOQJH010000001 DAOQJH010000000
VERSION     DAOQJH010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 19246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19246
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1304_length_19246_cov_7.754312"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(51..473)
                     /locus_tag="PLQ18_00005"
     CDS             complement(51..473)
                     /locus_tag="PLQ18_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007834656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF126 domain-containing protein"
                     /protein_id="HNE00056.1"
                     /translation="MKASVLQGRKVHGGIVEGEALVTTEAIGGYGAFDLDTGEVIEAN
                     HPLRGASVAGKVLVFSSAKGSSSWSVWHQALAINGVAPKAYVVRASNSQVALGAVVLR
                     IPTVTDLDRDPTEALESGDRVRVNGDEGTVEILQRKPR"
     gene            complement(470..1804)
                     /locus_tag="PLQ18_00010"
     CDS             complement(470..1804)
                     /locus_tag="PLQ18_00010"
                     /inference="COORDINATES: protein motif:HMM:NF016310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase X catalytic domain-containing protein"
                     /protein_id="HNE00057.1"
                     /translation="MISDIPDTLTSPRAAGTLRLGRDEQEMLDGKQGPARAKAMELLV
                     RYAEGLGAESFVDTNNVTLIAGTLPDIAVLRKAVPSLDIDAVASKFYLGSDEVVVVDK
                     VKAFTTSNATHRDQKYPDLQRGGAEACELMRRIEAYLKRIGVINLLTCTPYQSGNTPS
                     KGEHCAWIESSAQAFCNSVLGARTNIEGQHSSFASALTGKTPLWGMHLDENRHANVRV
                     EVEAQPVSAQDWYLMGYHFGTQLGQDIPVYTGVQRTPGRYSLIAFSAAGMTPGSVVMY
                     HIIGVTPEAATLEQACGNRRIERVLGYGPRERAQVYERLNRSDKDEVDIVILGCPHYG
                     LEQMKTVASMLEGKRIHGNVALYITTMRSVKAVLDREGYSDTLSAAGAIVLEDSCGLV
                     LNIMDPSAVVASDSAKMVHYVPGTTGVSKVWFGTTQECVDAAITGKWRGTLK"
     gene            complement(1855..2454)
                     /locus_tag="PLQ18_00015"
     CDS             complement(1855..2454)
                     /locus_tag="PLQ18_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014808314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid synthesis family protein"
                     /protein_id="HNE00058.1"
                     /translation="MSDVKVRKEFLVIEDTLREVGRDVEPRLRKVAAIAVVENPLAGR
                     YCEDLSMLVDFGEVLGEVLTRRAVDALGVPRGQVKGYGKAAIVGTAGELEHCAALIHP
                     KMGKPLRAAVEGGKAIIPSTKKRGGPGTRIDVPLFFKDDEWWMPYLDAMEINVPDAPA
                     PDEVVVAVVLSSGGRPLARLGTEQETKEQRERRAVGAAR"
     gene            complement(2503..2805)
                     /locus_tag="PLQ18_00020"
     CDS             complement(2503..2805)
                     /locus_tag="PLQ18_00020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE00059.1"
                     /translation="MTATNAGGDPVIAIVDGVAADLEAAQERLTQLDSALGDGDHGVT
                     MTIGARAVRKELGRLEGRGVGRAGSASGGHQDPGATSCYLILESIVRLVKQNASRP"
     gene            complement(2802..3806)
                     /locus_tag="PLQ18_00025"
     CDS             complement(2802..3806)
                     /locus_tag="PLQ18_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015256375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HNE00060.1"
                     /translation="MKKIINDPFEVVDEMLEGILLAHPGHLQRTGETGRALVRRGAPV
                     PGKVGIATGGGSGHLPVFLGYVGPGLADGVAVGNVFSSPGPQPMLDCIRAVDAGKGVL
                     LLFGNYQGDIMNFEAAAEMARADGIDVRIAIVNDDVPSAPRERIAERRGVAGLFYAYK
                     LVGARADEAVSLDELERTAREVIRNTRSVGVALAPCTVPAAGRPTFEIADDEMEFGMG
                     IHGEPGTERSRLKSADAIAAAMVETLLADLPFVAGDEVSVLVNGLGATPPTELYVLYR
                     RVHALLLEHGLRIDRSFVGEYATSLEMAGCSLTLLKLDDERKRLLHAPAFSPFLLQKS
                     "
     gene            complement(3837..4544)
                     /locus_tag="PLQ18_00030"
     CDS             complement(3837..4544)
                     /locus_tag="PLQ18_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014812389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNE00061.1"
                     /translation="MLLEASGIRTFYGSGQVLFDAALAVDRGEVVCLLGRNGAGKTTT
                     LRSIMGLTPPRAGSIRFEGEELVGRPPDYAARRGIGYVPDDRLIFPDLTVRENLDVGR
                     KPPRDPGAKPWTIDGIYELFPSLVKLDRRLGGFLSGGEQQMLTIGRTLMGNPSVLLLD
                     EPAEGLAPIVVRSLGDQILRLKQMGQTILLSEQNLPFAMRVADRAYVMSKGTIQFQGS
                     IAALAANDDIKRQYLMV"
     gene            complement(4544..5293)
                     /locus_tag="PLQ18_00035"
     CDS             complement(4544..5293)
                     /locus_tag="PLQ18_00035"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNE00062.1"
                     /translation="MTQQPILSLSGVSKNFGGLQASSNLDYVLHPGEFSALIGSNGAG
                     KSTFFNLITGKYPIDAGEIRYRDRRLNGLRPDQIARLGIARAFQVSNIYPTLSSYENV
                     RQAVLTRQRRTLDFFRSARSLGRAETEQLLEACGLATLAGVAAGTLSQGDKKRLELAV
                     ALGTEPDLLLLDEPTAGMSGEESRRIMAFVDELNRSRGLTILFTEHDMALVFGFARRI
                     SVLHQGSIIADGLPEDVRCNEMVQSVYLGES"
     gene            complement(5290..6261)
                     /locus_tag="PLQ18_00040"
     CDS             complement(5290..6261)
                     /locus_tag="PLQ18_00040"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNE00063.1"
                     /translation="MGWLVAAGVVAAVALPFAAGLFWVHVFTEILILGLFAMSFNLLF
                     GFTGQLSFGHAAYYGVGVYATALLLTKTSLPLAVCMAASIVLAGAFALAFGFFCVRLT
                     GIYFSILSLALGQFVYYVIFNWYEFTGGDNGIQGVSPPLALRDPYAYYYFTLVVVAAA
                     SVLMWRIVNSPFGYTLRAIRDNADRTRFIGIDVRRYVLICFVIAGMFAGLAGALWAPF
                     ARSVSPDLANWTQSGLPVFMTILGGPSHLLGPMVGSAIYTFLSAFVTGFTVYWPLTIG
                     TIIIFIVLLMPGGVLGAIDAAFAARRTRRRASNAEVVHGSRGEGVAE"
     gene            complement(6290..7174)
                     /locus_tag="PLQ18_00045"
     CDS             complement(6290..7174)
                     /locus_tag="PLQ18_00045"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNE00064.1"
                     /translation="MTASEILGQLLSGVSRGMVLFLIASGLTLTFSVMNILNFAHAAF
                     WLVGAYVTWTIWHLLLLPATGFWIALPAAALVMGAIGWIVEFVLIRRVYDRPHTEQLL
                     LTYALVLVIGDLIKLTWGVDDRIVGRPELLRGYVELLGAPLPLYSIFVIALGCFIAAA
                     LWWMLHRTRFGRIVRAAVYSREMVSALGLPIRRIYAGVLAISLFIAGLAGGVQSPISS
                     ITLGMDMNVIIEVFCIMVIGGLGSLAGAFVGSLIVGVTYSFAILVFPKMALVLIFLVT
                     ALVLIVRPWGLFGTPMRV"
     gene            complement(7307..8560)
                     /locus_tag="PLQ18_00050"
     CDS             complement(7307..8560)
                     /locus_tag="PLQ18_00050"
                     /inference="COORDINATES: protein motif:HMM:NF024825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNE00065.1"
                     /translation="MGSSLGLAATPLVLKSAHAAEPYRLGAVQALTGTGSVSSKHSLN
                     GIELAVEEINREGGFLARHPIQLFVRDDQTKPDIAAREAKDLILRDRVRSIIGSFSSH
                     LTLAIEEVAYEYKVLHITASANSESLSVGNYSPYTFLVVPNSYMEPKAQAKVIAKMAR
                     DKGWKTFATFASDYEWGHSNTDAFISEVQKAYPEFKLVKQFWPKAGETEFSSYVTAIM
                     SAKPDFVFASISGTDAYAWMRQARAYGFFEKIPYTALVALTEMMTLKDDLPRGIISVS
                     RAPFYANMDVPMMAAMVKNYRAKFDGLYPTDFAVMHYDAVYALKQAIEKAASIDTEKV
                     KAAMKGMTVETTRGKLRFRDIDNQMDAPSYVGIIADDPAYPFPITKDTVKFPGNETWR
                     PESEIPALRKASGLAKRRTAADLEV"
     gene            8816..9505
                     /locus_tag="PLQ18_00055"
     CDS             8816..9505
                     /locus_tag="PLQ18_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010909858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HNE00066.1"
                     /translation="MSLRPAGAHALSIGSMQLRKTTLQEHVYEQLAELILNGEIAPGQ
                     LVTIQALSDAFGVSAMPIREALKKMVGANALTVIASRSIGIPKLTAERLADLTRVRLE
                     IEGAAAEWAAARVTPEEIDRLERLLAEMLKAARSSDVKGFLRSNREFHFTVYRAAGSD
                     TAYGIIEKLWLQVAPYFHHLYALDNYRKANTEHKAIVSALKSGDAEAAREGIRADIET
                     GSKLLVAMLSQ"
     gene            complement(9530..11068)
                     /locus_tag="PLQ18_00060"
     CDS             complement(9530..11068)
                     /locus_tag="PLQ18_00060"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017773946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102662 - malonate-semialdehyde
                     dehydrogenase (acetylating, NAD+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HNE00067.1"
                     /translation="MNAASEHGAPVRRHDDPGTVGHFIGGRRIAGTGATSPVYDPATG
                     ERAREVALATQVEVESAVAAARAASADWADTPPVRRARLMNAFLALMQRDRDRLAAMI
                     TAEHGKTFADAQGEVSRGIDVIEFACGAPQLLKGDYTEQVSGGMDNWTMRQPLGVVAG
                     ITPFNFPVMVPCWMFPVAIACGNTFVLKPSERDPSASVHVAGLLREAGFPDGVFNVVQ
                     GDKTAVEALLDHPDVAAVSFVGSTPVARHVYETGARHGKRVQALGGAKNHMVVMPDAD
                     LDQAVDALIGAAYGSAGERCMAISVAVLVGDVADRIVPRLAERARTLRVRDGMQADAQ
                     MGPLVTREALERVHRYIALGVEEGATLVVDGRGLQVPGHANGFFTGGTLFDHVKPTMR
                     LYREEIFGPVLGCVRVSDLAEAIALVDGHEFGNGVSCFTRDGHAAREFARRIQVGMVG
                     INVPIPVPMAWHGFGGWKRSLFGDVHAYGEEGVRFYTRQKSVMQRWPASVARGAEFSM
                     PTPR"
     gene            11153..12142
                     /locus_tag="PLQ18_00065"
     CDS             11153..12142
                     /locus_tag="PLQ18_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNE00068.1"
                     /translation="MNNSEVSVASGDDVFRSDLVWSRVHALTAVARCGSFTTAARQLG
                     VSKAAVSQRVNELERTVGAALLQRTTRSVRLTHVGQQLVDDTSECFSRIESSVAAARD
                     LAAQPRGVLRVSAPVALGRQYVAPSLEPFLRAYPQIRIELELSDVLVHLAQEGFDLAI
                     RHTSSPPENHVATRLAATRTLLVASAGYLRRRGSPSQPAQLAEHDCLAYLRPGPALWH
                     FEADGPAAHGAHDGARASVPVRGPLRTNNSEVLRDAVLSGLGIAQLPDFSAAPALRAG
                     RLREVLGGWRPVGFFGDSIYALRPWSRGTPRSVRLLVEHLREELRGGFLQAAA"
     gene            complement(12194..13186)
                     /locus_tag="PLQ18_00070"
     CDS             complement(12194..13186)
                     /locus_tag="PLQ18_00070"
                     /inference="COORDINATES: protein motif:HMM:NF015366.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HNE00069.1"
                     /translation="MKRALFQRAMAACAVLASMHAIGLPTAHAQASFPERPVHIVVPF
                     LAGGSVDTVARLIGDKLGPRLGQQVVVENKAGAGGVVGADFVARARPDGHTLLLTPPG
                     PLTTNAVLLKSMPYDARKAFDPVSLVAALPNVLLVGEKRGEWTAQKIIAEAKANPEKL
                     TYGSQGIGTTGHLTGVLFDQQTGARLTHVAYKGFPPVLVDVIAGRVDMMFVDTANALP
                     RVRNHSLIALAVASKSRVSSLPDVPTFTELGYPGVVSDTWFALMAPAGTPQAIRQRLR
                     DEVAAILKDPEVLDRLRELGVEPVGDQPDELAEHIRKEYERWRTIIESAGLGPK"
     gene            complement(13228..14874)
                     /locus_tag="PLQ18_00075"
     CDS             complement(13228..14874)
                     /locus_tag="PLQ18_00075"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HNE00070.1"
                     /translation="MARYTPETVARYEQEGYWTRQYPVDFWQENALRFPERDALVFGT
                     RRFTWKAAAQAIDRLAVGLVHCGFAKDDVLALQAPNSATLMLLRLAAEEAGVISLLVP
                     PTFSRAEVGAIAGRLPLAGAVLPNTERARELAEVYRAGAAPSFRLLSIGESPLPGAID
                     VESWLARSCAPGEPARVLEGRRFRPYEYAAIITTSGTTGAPRFVEHTACARSASGRVY
                     IDRLRLGCDDVVAAMVSVFAGNCDLVVHHTAPQVGARVVLVDRFDPESACRLLESERV
                     TCAVFVPTLLHRLLAYEGLRRHDLSSLRIVTSFGAILAPEIAAEVERVLGVKVIQGYG
                     ASDYGSLASTSIDDPQRVRLAGVGRPLTGTELRICDEHGTALEPGVPGRIFARGPHCV
                     GGFVRDEAATAQAWESGYCAMGDFGHIDDEGYLWLSGRVRELVIRGGQNIVPAEIEDA
                     ILGHPGVAEVAVIGVPDAEMGERVCAAIVPRAGARLTLESITAHLGERGLARFKHPER
                     LLVLETMPLNPAATKIDKRALRALALGHRTSDRSEAVSRA"
     gene            complement(14905..16896)
                     /locus_tag="PLQ18_00080"
     CDS             complement(14905..16896)
                     /locus_tag="PLQ18_00080"
                     /inference="COORDINATES: protein motif:HMM:NF012927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNE00071.1"
                     /translation="MLTPLLFEPITIGGLRVPNRLTMPPMHANLGSMQEGITDRGIDF
                     YVARAKGGFGLMGVGVIDAFFVDHAGSPHELFLDNDLHVQRYARLVQELRRHGTVPYA
                     QIGIRRLFHVSRLHREKATDRPSLAEIPAEQIEEMIEAVVAASVRAARAGFMAVDILG
                     VGGSGHSLFASRVFNDRCDAWGGTPEKRIRFAVETVRGIKKALGEDFPVFYRLHGSEF
                     LPGGYGIEGAQFNAVQLERAGVCFFNVTGGGHATSVPQLTPNVPQGAYAYLAREIKRV
                     VGSAAVAASNRNNHPLEAERILRHGWADLISLGRQSLADADWPLKAREGRFSDIRYCI
                     ACNECMDTAVIRNEPVRCLVNPREGAASEVEPIPMARVRRRVVVVGGGVAGLQFALTS
                     AERGHEVTLIERKGHLGGMWQHASTPPGREQLFTFLEWLVGQCAKAGVAFRMQTEATA
                     DLLRAMNADLIAVSPGSEPVESSLPGADLPHVHPATAALEGRIPIGERVVVIGGGGIG
                     VEIAPFLARRWQVRTEVLDFLDDYQALADNDREILDRKGHHVTLVSRQRRLGGSVGGS
                     TRWVLGKEVAIAGVEILVNAVAKEITASEVVVEQGNEEIRIPADTVFLATGLNPDTRL
                     YEQLTASKAAPRVELIGDPEQAMHAIESVSQAFRLALEV"
     gene            16989..17492
                     /locus_tag="PLQ18_00085"
     CDS             16989..17492
                     /locus_tag="PLQ18_00085"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNE00072.1"
                     /translation="MQENAQSSRRDGLLPLEQRFTYRFALVASRMGTALAPSHERYGL
                     SVGTWRVLAVIARYEPLSAKELAQRTSTDPFRVTRALTMLAEKGLVSRQADPKDRRRV
                     SLRLTRAGRGVHDEIAATLSEMERSVLVALSAREQAMLHRLLDKLDVRIAACLQEQSW
                     SVQGPPR"
     gene            17599..18540
                     /locus_tag="PLQ18_00090"
     CDS             17599..18540
                     /locus_tag="PLQ18_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HNE00073.1"
                     /translation="MKPRILQSARLMPALEARLRSEFEAHPLWNEPDPAAFLEARGGE
                     FEILVTNATAGASASMMDALPALKAICSLGVGCDAIDLQAARERGVVVANTPDVLTDC
                     VADIAMGLLIDVARGLSAADRHVRRGKWLEGPFPLTTRVTGKRLGILGLGRIGQAVAR
                     RARGFEMQLRYHSRRPVASSNLVYEPSLVQLAQWADFLVVACSGGAATQGLVSEAVLR
                     ALGPQGFLVNVARGSVVDEAALVRALQQGTIAGAGLDVYADEPNVPPALIALENAVLL
                     PHMASGTRETRQAMADLVFENVQSFVEHGRLKTPVPA"
     gene            complement(18607..19212)
                     /locus_tag="PLQ18_00095"
     CDS             complement(18607..19212)
                     /locus_tag="PLQ18_00095"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HNE00074.1"
                     /translation="MKTRRPHGGVFTATEAAGMGAFGGFVFALARGQLTPRKFSEVLV
                     ESARTTGMLFMILIGALIFANFVNFTTLPGDLQQAVQRFDLSPITVILAICALYIVLG
                     CVLESMSMLLLTVPVFYPLVQHLGFDLIWFGIIVVVVMEISFITPPVGMNVIVLRGLL
                     PDVSIRTMFRGVAPFVVADVFRLGILIAFPVISTLLPRYLG"
BASE COUNT         3082 a         6709 c         6471 g         2984 t
ORIGIN      
        1 ggcgccgcgc gccgcgggcg ctcgcgcgcg gctcgggagc gcggcgcgcg tcagcgcggc
       61 ttgcgctgca ggatctcgac cgtgccctcg tcgccgttca cccgcacgcg gtcgcccgat
      121 tcgagcgcct cggtcggatc gcggtcgagg tcggtaaccg tggggatgcg cagcacgacc
      181 gcacccagcg cgacctgcga attcgacgcg cgaaccacgt aggccttcgg cgcgacgccg
      241 ttgatcgcga gcgcctggtg ccagaccgac cacgacgaag atcccttcgc gctcgagaac
      301 acgaggacct tgccggcgac gctcgcgccg cgcagcgggt ggttcgcctc gatgacctct
      361 ccggtatcga ggtcgaacgc accgtagccg ccgattgcct ccgtggtcac gagcgcctcg
      421 ccttcgacga ttccgccgtg aacctttcgc ccctgcagca ccgatgcctt catttcagcg
      481 tgcctcgcca tttgccggtg atcgccgcgt ccacgcattc ctgcgtcgtc ccgaaccaga
      541 ccttgctcac ccccgtggtg ccgggaacgt aatgcaccat cttcgcggaa tcggaggcga
      601 cgacggccga cggatccatg atgttgagca ccagtccgca ggagtcctcg agcacgatgg
      661 cgccggcggc gctgagcgtg tcggagtaac cctcgcggtc gagcaccgcc ttcaccgatc
      721 gcatcgtcgt gatgtacagc gccacgttcc cgtggatcct tttgccttcg agcatcgagg
      781 ccaccgtctt catctgctcc aggccgtagt gcgggcagcc gaggatcacg atgtcgactt
      841 cgtccttgtc ggaacggttc agccgctcgt acacctgtgc gcgctcgcgc ggcccgtagc
      901 ccagcacccg ctcgatgcgg cggttcccgc acgcctgctc gagcgtcgcc gcctcgggag
      961 tgacgccgat gatgtggtac atcacgacgc tccccggcgt catgccggcc gcgctgaacg
     1021 cgatcaggct gtagcgcccc ggcgtgcgct gcacgcccgt gtagacggga atgtcctggc
     1081 cgagctgcgt gccgaagtgg tagcccatga ggtaccagtc ctgcgcgctc accggctgcg
     1141 cctcgacttc gacccgcacg ttcgcgtggc ggttctcgtc gaggtgcatg ccccacaggg
     1201 gcgtcttccc cgtgagggcg gaggcgaacg agctgtgctg cccctcgatg ttcgtgcgcg
     1261 cgccgagcac cgaattgcag aacgcctgcg cggacgattc gatccaggcg cagtgctcgc
     1321 ccttgctcgg cgtgtttccc gactggtagg gcgtgcaggt gagcaggttg atgacaccga
     1381 tgcgcttcag gtaggcctcg atcctgcgca tcagctcgca cgcctcggcg ccgccccttt
     1441 gcaggtcggg atacttctgg tcacggtgcg tggcgttgct cgtcgtgaac gccttcacct
     1501 tgtcgacgac cacgacctcg tcgctgccga ggtagaactt cgacgcgacg gcatcgatgt
     1561 cgagcgaggg caccgccttt cgaagcacgg cgatgtcggg aagcgtgccg gcgatcagcg
     1621 tgacgttgtt cgtgtcgacg aacgattcgg cgccgagccc ctcggcgtat cgcacgagca
     1681 gctccatggc tttcgcacgc gcgggtccct gctttccgtc gagcatttcc tgctcgtcgc
     1741 ggcccaggcg caacgtgccc gcggcgcgcg ggctcgtgag ggtatcggga atatcggaaa
     1801 tcatcggatc caacctcctg cgcgcgaagc ccctcgaggc cccgcgcaaa gggactacct
     1861 cgcggcgccc accgcgcgac gttcgcgctg ctccttcgtt tcctgctccg tgccgagccg
     1921 tgcgagcgga cgtccgccgc tgctgagaac gacggcgacg acgacttcgt cgggcgcggg
     1981 cgcatcgggc acgttgatct ccattgcgtc cagatacggc atccaccatt cgtcgtcctt
     2041 gaagaacagc ggaacgtcga tgcgggttcc cggacccccg cgcttcttgg tcgacggaat
     2101 gatcgccttg ccgccttcga cggccgcgcg cagcggcttg cccatcttcg gatggatgag
     2161 cgccgcgcaa tgctcgagct cgccggcggt gccgacgatg gcggccttgc cgtagccctt
     2221 gacctgcccg cgaggcacgc ccagcgcgtc caccgcgcga cgcgtgagca cctcgccgag
     2281 aacctcgccg aagtcgacga gcatggacag gtcttcgcaa tagcgcccgg ccagcggatt
     2341 ctccacgacc gcgatggcgg ccaccttgcg caggcgcggt tcgacgtcgc ggcccacttc
     2401 gcgcaacgtg tcttcgatga ccagaaactc ctttcgcacc ttgacgtcac tcaccaccac
     2461 gactccttgt tccacgggaa atgaaaggcg ggcacggcac gctcatggcc gactcgcgtt
     2521 ctgcttcacg agtcgcacga tcgactcgag aatgaggtag caggacgtgg cacccgggtc
     2581 ctgatggccc ccgctcgccg agccggctcg cccgacgcct cgaccctcga gcctgcccag
     2641 ctccttgcgc acggcgcgcg cgccgatcgt catcgtcacc ccgtggtcgc cgtccccgag
     2701 tgccgaatcg agctgcgtca ggcgttcctg cgcggcctcg agatcggcgg caacgccatc
     2761 gacgatggcg atcaccggat cgccgcctgc attcgtcgcc gtcatgattt ctgcagcagg
     2821 aaaggcgaga aggccggcgc gtgcaggagc cgcttgcgct cgtcgtcgag cttgagcagc
     2881 gtgagcgagc agcccgccat ctcgagcgag gtcgcatact cgccgacgaa gctgcggtcg
     2941 attctcaggc cgtgctcgag cagcagcgcg tgcaccctgc gatacaggac gtagagctcg
     3001 gtgggcggcg tcgcgcccag cccgttcacg agcaccgaca cctcgtcacc ggcgacgaaa
     3061 ggcaggtcgg ccagcagcgt ctcgaccatt gccgcggcga tcgcgtcggc cgacttcagc
     3121 cgggagcgct cggtccccgg ctcgccgtgg atgcccatcc cgaattccat ctcgtcgtcg
     3181 gcgatctcga acgtgggacg cccggcggcc ggaaccgtgc acggcgcgag cgcaacgccc
     3241 acgcttcgcg tgttccggat cacctcgcgc gcggtgcgct cgagctcgtc gagcgacacc
     3301 gcctcgtcgg cgcgggcgcc cacgagcttg taggcgtaga agagacccgc cacgccgcgg
     3361 cgttcggcga tgcgctccct cggcgccgac ggcacgtcgt cgttgacgat tgcgatccgt
     3421 acgtcgatgc cgtcggcgcg cgccatctcg gctgccgcct cgaagttcat gatgtcgccc
     3481 tggtagttgc cgaacagcag caacacgccc ttgccggcat cgacggcacg gatgcaatcg
     3541 agcatcggct gcggccccgg agacgagaac acgttgccca ccgcaacgcc gtcggcgagt
     3601 ccggggccca cgtagccgag gaagacgggc agatgccccg atcccccacc cgtggcgatg
     3661 cccacttttc ccggcaccgg cgcgcctcgg cgcacgagcg cgcggccggt ctctccggtg
     3721 cgctgcagat gcccggggtg ggccagcagg atcccctcga gcatctcgtc gacgacttcg
     3781 aacggatcgt tgatgatttt cttcacgatg actcctggat tcggctggaa cgggcttcag
     3841 accatcaggt actgccgctt gatgtcgtcg ttcgccgcca gcgccgcgat gcttccctgg
     3901 aactggatcg tccccttgct catcacgtag gcgcggtcgg ccacgcgcat cgcgaacgga
     3961 aggttctgct ccgagagcag gatcgtctgt cccatctgct tgagccgcag gatctggtcc
     4021 cccaggctgc gcacgacaat gggcgcgagc ccctcggcgg gctcgtcgag caggagcacc
     4081 gaaggatttc ccatgagcgt gcggccgatg gtcagcatct gctgctcgcc gccgctcagg
     4141 aaacccccga gccgccggtc gagcttgacc aggctcggga acagctcgta gatgccgtcg
     4201 atcgtccacg gcttcgcgcc gggatcgcgt ggcggctttc ggccgacgtc gaggttctcg
     4261 cgcaccgtga ggtcgggaaa gatcaggcgg tcgtcgggga cgtagccgat gccgcgccgc
     4321 gccgcgtagt cgggcgggcg tccgacgagc tcttcgccct cgaagcggat gctgcccgcg
     4381 cgcggcgggg tgagccccat gatgctgcgc agcgtcgtcg tcttccccgc gccgttcctg
     4441 cccagcaggc atacgacttc gccgcggtcg acggcgagcg ccgcatcgaa gagcacctgg
     4501 cccgagccgt agaacgtgcg gattccgctc gcttcgagga gcatcacgat tcgcccaggt
     4561 agacgctctg caccatctcg ttgcaccgca cgtcttcggg caacccgtcg gcgatgatcg
     4621 agccctggtg cagcaccgag atccggcgcg cgaagccgaa cacgagtgcc atgtcgtgct
     4681 cggtgaagag aatcgtcagg ccgcgactgc ggttcagctc gtcgacgaac gccatgatcc
     4741 tgcggctctc ctcgcccgac atgcccgcgg tcggctcgtc gaggagcagc aggtcgggct
     4801 cggtgccgag cgcgacggcc agttcgagcc gtttcttgtc gccctgcgag agcgtgccgg
     4861 ccgcgacgcc ggcgagcgtc gcgagcccgc acgcctcgag aagctgctcg gtctcggcac
     4921 gccccagcga gcgggccgac cggaagaagt cgagcgtgcg acgctgccgc gtgagcaccg
     4981 cctggcgcac gttctcgtag ctcgacagcg tcgggtagat gttgctgacc tggaacgccc
     5041 tcgcgatccc gagacgcgcg atctggtccg ggcgaagacc gttcagccgc ctgtcgcggt
     5101 agcggatttc gccggcgtcg atcggatact tgccggtgat caggttgaag aaggtgctct
     5161 tgcccgctcc gttggatccg atcagtgccg agaattctcc cgggtgcagg acgtagtcga
     5221 gattcgagga ggcctgcagg ccgccgaaat tcttgctcac gcccgagagg ctgaggatcg
     5281 gctgctgcgt cactccgcga ccccctcgcc gcgcgaaccg tgcacgacct cggcgttgct
     5341 cgcgcgcctg cgcgtgcgcc gcgcggcgaa cgcggcgtcg atcgcgccga gcacgcctcc
     5401 gggcatcagc aggacgatga agatgatgat cgtgccgatc gtgagcggcc agtacaccgt
     5461 gaagccggtc acgaaggcgg agaggaacgt gtagatcgcc gagccgacca tcggcccgag
     5521 caggtgcgag ggtccgccca ggatcgtcat gaagaccgga agacccgact gcgtccagtt
     5581 cgcgaggtcc ggggagacgc tgcgcgcgaa aggcgcccac agcgcaccgg ccaggcccgc
     5641 gaacatgccg gcgatgacga agcagatcag gacgtagcgc cgcacgtcga tgccgatgaa
     5701 tcgcgtgcga tcggcgttgt cgcggatggc tcgcagcgtg tagccgaacg gcgagttcac
     5761 gatgcgccac atcagcaccg aggcggccgc cacgacgacg agggtgaagt agtagtaggc
     5821 gtacggatcg cgcagcgcga gcggcggcga gacgccctgt attccgttgt cgccgcccgt
     5881 gaactcgtac cagttgaaga tcacgtagta gacgaactgc ccgagcgcga gcgaaaggat
     5941 cgagaaatag atgcccgtca gccgcacgca gaagaaaccg aaggcgagcg cgaaggcgcc
     6001 ggccagcacg atcgacgcgg ccatgcacac ggccagcggc aacgaggtct tcgtcagcag
     6061 cagcgccgtg gcatagacac cgaccccgta gtacgcggcg tggccgaacg agagctgccc
     6121 cgtgaatccg aacagcaggt tgaagctcat cgcgaacagg ccgaggatca ggatctcggt
     6181 gaacacgtgg acccagaaca ggcccgcggc gaagggcaat gcgacggctg cgacgacgcc
     6241 ggcggcgacg agccagccca cggtggaggc gccggagtct ctcatcgact cagacccgca
     6301 tcggcgtgcc gaacaggccc cacggccgga cgatcagcac cagggcggtc accaggaaga
     6361 tgagcacgag cgccatcttc gggaacacga ggatcgcgaa cgaatacgtg acgccgacga
     6421 tcagcgagcc gacgaacgca cccgcaagac tgcccaggcc gccgatcacc atgatgcaga
     6481 acacctcgat gatcacgttc atgtccatcc cgagggtgat cgacgagatg ggcgactgca
     6541 cgcctcccgc cagccccgcg atgaacaggc tgatggcgag gaccccggcg tagatccgcc
     6601 ggatcggcag ccccagcgca ctcaccatct cgcggctgta gaccgccgcc cggacgatcc
     6661 ggccgaagcg ggtccggtgc agcatccacc acagcgccgc cgcgatgaag cagccgagcg
     6721 cgatcacgaa gatgctgtac agcggcagcg gcgcaccgag cagctcgacg tagccgcgca
     6781 gcagctcggg ccggcccacg atgcggtcat cgacgcccca cgtcagcttg atcaggtctc
     6841 cgatcacgag cacgagcgca taggtgagca gcagctgctc ggtgtgcggg cgatcgtata
     6901 cgcggcggat gaggacgaac tcgacgatcc agccgatggc gcccatcacc agcgcggccg
     6961 ccggcagtgc gatccagaag ccggtggccg gcagcagcag caggtgccag atcgtccagg
     7021 tgacataggc acccacgagc cagaacgccg cgtgcgcgaa gttcaggatg ttcatgacgc
     7081 tgaaggtcag cgtgagtccg gaggcgatga ggaacagcac cattcccctc gagaccccgc
     7141 tgagcaactg gcccaggatt tcgctcgccg tcattcgatg ctcgtcaggt cgtcgcgtct
     7201 cgcgagatcc gatcgccgag gcgccggaca agcgccggtg aagcgcttgc acccgcatgc
     7261 cgggcaaccc ggcgccgcgg caatcgccgt catcggcctg gcccgcctag acctccaggt
     7321 cggcagcggt gcgtcgcttc gccaggcccg aggccttgcg cagcgccgga atctccgatt
     7381 ccggacgcca cgtctcgttg cccgggaact tcacggtgtc cttcgtgatc gggaacggat
     7441 aggccggatc gtcggcgatg atgccgacgt acgaaggcgc atccatctgg ttgtcgatgt
     7501 cacggaaacg cagcttgccg cgcgtcgtct cgacggtcat ccccttcatg gccgccttca
     7561 ccttttcggt gtcgatgctc gcggccttct cgatggcctg cttcagcgcg tagacggcgt
     7621 cgtagtgcat gaccgcgaaa tcggtcggat acagcccgtc gaacttcgcg cgatagttct
     7681 tgaccatcgc cgccatcatc gggacgtcca tgttcgcgta gaagggcgcg cggctcaccg
     7741 agatgattcc gcgcggcagg tcgtccttga gcgtcatcat ttcggtgagc gcgaccagcg
     7801 ccgtgtaggg gatcttctcg aagaacccgt aggcgcgcgc ctggcgcatc cacgcgtagg
     7861 cgtcggttcc cgaaatcgaa gcgaagacga aatcgggctt cgccgacatg attgcggtga
     7921 cgtacgagga gaactcggtc tcaccggcct tcggccagaa ctgcttgacc agcttgaact
     7981 ccgggtacgc cttctgcact tcgctgatga aagcgtcggt gttgctgtgg ccccattcgt
     8041 agtcggacgc gaacgtcgcg aaggtcttcc agcccttgtc gcgggccatc ttcgcgatga
     8101 ccttcgcctg cgccttgggc tccatgtagc tgttggggac gacgaggaac gtgtaggggc
     8161 tgtagttgcc gaccgacagg ctctcggagt tcgccgaagc cgtgatgtgc agcaccttgt
     8221 actcgtaggc gacttcctcg atcgccagcg tcaggtgact gctgaacgac ccgatgatgc
     8281 tgcggacccg gtcgcgcaga atgaggtcct tcgcttcgcg cgccgcgatg tcgggcttgg
     8341 tctggtcgtc gcggacgaac agctggatcg ggtgccgtgc gaggaagccg ccctcgcggt
     8401 tgatctcctc gaccgcgagc tcgatgccgt tcaggctgtg cttcgacgag accgacccgg
     8461 tgccggtgag cgcctgcacg gcgccgaggc gatacggctc ggccgcgtgc gccgacttca
     8521 ggacgagcgg cgtcgcggcc aggcccagcg agctgcccat ggccagccgc gtgaattccc
     8581 tacgtttcat gctccctcct cggtggactg cccgtgcatg gactgcaagt gccttgcttg
     8641 tgtctgatca tacatctatg atcaaatcat cgtcaacgcg ccgattcccg gcgaggcgac
     8701 tcggccgatg gcgcttttga ccagtgatca ctaatctata tactcgcgcg tcaccttaac
     8761 ggcacgcgcc ggcccggagc gaatcgggcg agtccacggt ggggtcaacg aacgaatgag
     8821 cctgcgccca gctggcgccc atgcgctttc catcggctcg atgcagcttc gcaagacgac
     8881 gctgcaggag cacgtctacg agcagctcgc cgagctgatt ctcaacggcg aaatcgcccc
     8941 gggccagctc gtgacgatcc aggccctctc cgacgcgttc ggcgtcagcg cgatgccgat
     9001 tcgcgaggcg ctgaagaaga tggtcggcgc caacgcgctc accgtcatcg cgagccgctc
     9061 gatcggcatc ccgaaattga cggccgagcg gctcgccgac ctcacgcgcg tgcgcctcga
     9121 gatcgaaggc gccgcagccg agtgggccgc cgcgcgcgtg acgcccgagg agatcgatcg
     9181 gctcgagcgc ctgctcgccg agatgctcaa ggccgcgcgc agcagcgacg tcaagggctt
     9241 cctgcgctcg aaccgcgaat tccacttcac ggtctacagg gccgcgggtt ccgacacggc
     9301 ctacgggatc atcgagaagt tgtggctgca ggtggcgccg tatttccatc acctctacgc
     9361 actcgacaac taccgcaagg ccaacaccga gcacaaggcg atcgtctcgg cgctgaagtc
     9421 cggcgacgcc gaagcggcgc gcgaaggcat ccgcgccgac atcgagaccg gcagcaagct
     9481 gctcgtggcg atgctctcgc agtaacccgc cacgaccccg cgtgccgcct cagcgcggcg
     9541 tcggcatgct gaattcggcg ccgcgcgcca cgctggccgg ccagcgctgc atcacgctct
     9601 tctgcctcgt gtagaagcga acgccctcct cgccgtaggc gtgcacgtcg ccgaagaggc
     9661 tcctcttcca gccgccgaag ccgtgccatg ccatcggcac cggaatcggc acgttgatgc
     9721 cgaccattcc gacctgaatg cgacgcgcga attcgcgtgc ggcgtgcccg tcgcgcgtga
     9781 agcacgaaac gccgttgccg aactcgtggc cgtcgacgag cgcgatcgcc tcggcaagat
     9841 cgctcactcg cacgcaaccg agaacgggtc cgaagatctc ttcgcgatac aggcgcatcg
     9901 tcggcttcac gtggtcgaag agcgtccctc cggtgaagaa gccgttcgca tgccccggca
     9961 cctggagtcc gcgcccgtcg acgacgagcg tggcgccctc ctcgacgccc agcgcgatgt
    10021 agcggtgcac gcgctcgagc gcctcgcgcg tcaccagcgg tcccatctgc gcatcggctt
    10081 gcatgccgtc gcgcacccgc agcgtccttg cccgctcggc gagcctgggc acgatccggt
    10141 cggcgacgtc gcccaccagc acggccacgc tgatcgccat gcagcgctcg cccgccgagc
    10201 cgtaggcggc gccgatcagc gcgtccacgg cctggtcgag gtccgcgtcg ggcatcacga
    10261 ccatgtgatt cttggcgccg ccgagcgcct ggacccgctt tccatggcgc gcacccgtct
    10321 cgtagacgtg ccgcgcgaca ggcgtggatc cgacgaagct cacggcggcg acgtccgggt
    10381 gatcgagcag cgcctcgact gccgtcttgt caccctgcac gacgttgaag acgccgtcgg
    10441 gaaatccggc ctcgcgcagc aacccggcca cgtggacgct tgccgacgga tcgcgctcgc
    10501 tgggcttgag cacgaaggtg ttgccgcagg cgatcgccac cggaaacatc cagcagggga
    10561 ccatcaccgg gaaattgaac ggcgtgatgc cggcgacgac gccgagcggc tggcgcatcg
    10621 tccagttgtc catgccgccg ctgacctgct cggtgtagtc gcccttgagc agttgcggag
    10681 cgccgcaggc gaactcgatg acgtcgatcc cgcgcgagac ttctccctgc gcgtcggcga
    10741 aggtctttcc gtgctcggcg gtgatcatcg ctgcgagccg atcgcggtcg cgctgcatga
    10801 gcgcgaggaa cgcgttcatc aggcgcgcac ggcgcaccgg cggggtgtcg gcccagtcgg
    10861 cagacgcggc gcgcgccgcc gcgacggcgc tctcgacctc gacctgcgtg gccagcgcga
    10921 cttcgcgcgc gcgctcgccc gtggccgggt cgtagaccgg cgaggtcgcg cccgtgccgg
    10981 cgatgcgccg tccgccgatg aagtgaccca cggtgccggg gtcgtcgtgg cggcgcaccg
    11041 gggcgccgtg ctcgctcgct gcattcatgt cgtctctccc gatgaacgct gggaagtcta
    11101 ggcgcaggcg cttcgcagcg ggtacgcttg tgtgacaata ccgttcactg tagtgaacaa
    11161 tagcgaggta tcggtggctt ccggcgacga cgtctttcgc agcgacctcg tctggtcgcg
    11221 cgtgcacgcg ctgaccgccg tggcgcgatg cggaagcttc acgacggcgg cacgccagct
    11281 tggcgtgtcg aaggccgcgg tgagccaacg cgtcaacgag ctcgagcgca cggtcggcgc
    11341 tgccctgttg cagcgcacga cgcggtcggt gcgcctgacg cacgtcgggc aacaactggt
    11401 cgacgacacg agcgaatgct tctcgcgaat cgagtcgagc gtcgccgcgg cgcgcgacct
    11461 cgccgcccag ccacgcggcg tgctgcgcgt ctcggcgccc gtggcgctcg ggcggcaata
    11521 cgtggcaccg tcgctcgagc cgttcctgcg cgcgtacccg cagatccgca tcgagctcga
    11581 gctctccgac gtgctcgtgc acctcgccca ggagggcttc gacctggcca ttcgccacac
    11641 gagctcgccg cccgagaacc acgtcgccac gcggctcgcc gcgacgcgca cgctgctcgt
    11701 ggccagcgcc ggctacctgc gccgacgcgg cagcccgtcg cagcccgcgc agctcgccga
    11761 gcacgattgc ctcgcctacc tgcgcccggg cccggcgctc tggcacttcg aggccgacgg
    11821 tccggcggca cacggcgcgc acgacggcgc acgcgcgagc gttcccgtgc gcgggccgct
    11881 gcgaaccaac aattccgaag tccttcgcga tgcggttctc tcggggctgg ggatcgcgca
    11941 gctacccgac ttcagcgcgg cgccggcgct gcgcgccggc cggctgcgcg aagtcctcgg
    12001 cggctggcgt cccgtgggct tcttcggcga ttcgatctac gcgctgcgtc cctggagtcg
    12061 cggcacgccg cgctcggtgc gcctgctcgt cgagcacctg cgcgaggaac tgcgcggggg
    12121 cttcctgcag gccgccgcct gagcgaaggc ggctcagcgc acgagccccg ggtacagccg
    12181 cacggccgag ccgctatttc ggcccgaggc cggcgctctc gatgatcgtg cgccagcgct
    12241 cgtattcctt gcggatgtgc tcggcgagtt cgtcgggctg atcgcccacg ggctccacgc
    12301 cgagttcgcg cagccgatcg aggacttcgg gatccttcag gatcgccgcc acttcgtcgc
    12361 gcaggcgctg gcggatcgcc tgcggcgttc ccgcgggcgc catcagcgcg aaccaggtgt
    12421 ccgagacgac gccgggatag ccgagctcgg tgaaagtggg cacgtcgggc agcgacgaga
    12481 cgcggctctt cgaggcgact gccagcgcga tcagcgaatg gttgcgtacg cgcggaagcg
    12541 cgttcgcggt gtcgacgaac atcatgtcga cgcggcccgc gatcacgtcc acgagcaccg
    12601 gtgggaaacc cttgtacgca acgtgcgtga gcctggcgcc ggtctgctgg tcgaacagta
    12661 cgcccgtcag gtgcccggtc gtgccgatgc cctgcgaacc gtaggtgagc ttctcggggt
    12721 tcgccttcgc ctcggcaatg atcttctgcg cggtccattc gccccgcttc tcgccgacga
    12781 gcagcacgtt cggcaatgct gcgacgagcg agaccgggtc gaacgccttg cgcgcgtcgt
    12841 acggcatgct cttgagcagg accgcgttcg tggtgagcgg ccccggcggc gtcaacagca
    12901 gcgtatggcc atcgggacgc gcgcgcgcga cgaagtcggc tccgacgacg ccaccggcac
    12961 cggccttgtt ctcgacgacg acctgctggc cgagccgcgg cccgagcttg tcgccgatga
    13021 gccgcgcaac cgtgtcgacc gatccgccgg cgaggaacgg cacgacgatg tgcacgggcc
    13081 gctcggggaa cgacgcctgc gcgtgcgccg tgggcagccc gatcgcatgc atcgaggcca
    13141 gcaccgcgca ggcggccatg gccctttgga acagcgctcg cttcatgggt ctcctccttt
    13201 cgtgccgcct tcgggcggcc gtcgcgttca cgcccgggaa actgcctccg acctgtcgct
    13261 ggttcgatgt ccgagcgcca atgcgcgcag cgcgcgcttg tcgatcttcg tcgctgccgg
    13321 attcagcggc atcgtctcga gcacgagaag gcgctcggga tgcttgaacc gggcaagccc
    13381 gcgttcgccc agatgcgcgg tgatcgattc gagcgtgagg cgtgcgcccg ccctgggcac
    13441 gatcgccgca cagacgcgct cgcccatctc ggcgtcggga acgccgatca cggccacctc
    13501 ggcgacgccg ggatgtccga gaatcgcatc ttcgatctcg gcgggaacga tgttctgtcc
    13561 gccgcggatc acgagctccc gaacgcggcc cgagagccag agatagcctt cgtcgtcgat
    13621 gtggccgaaa tcgcccatcg cgcagtaccc cgactcccag gcctgcgccg tggcggcttc
    13681 gtcgcgcacg aagccgccca cgcaatgtgg accgcgcgca aagatgcgcc ccggcacgcc
    13741 gggctcgagc gccgtgccgt gctcgtcgca gatgcgcaat tcggtgccgg tcaacggacg
    13801 tccgacgccg gccaggcgca cgcgctgcgg atcgtcgatc gacgtgcttg ccagcgaccc
    13861 gtagtcggac gccccgtagc cctggatgac cttcacgccg agcacccgct cgacctcggc
    13921 cgcgatctcc ggcgcgagga tcgccccgaa gctcgtgacg atgcgcaacg acgagaggtc
    13981 gtgccgccgc aggccctcgt aggcgagcag gcggtgcagc agcgtcggca cgaacaccgc
    14041 acaggtgacg cgttcggact cgaggaggcg acaggcggat tccggatcga agcgatcgac
    14101 gagcacgact ctcgccccga cctgcggcgc cgtgtggtgc acgacgagat cgcagttgcc
    14161 cgcgaagacc gacaccatcg cggcgacgac gtcgtcgcag ccgagccgca gccggtcgat
    14221 gtagacgcgc ccggacgccg agcgcgcaca ggccgtgtgc tcgacgaagc gaggcgcgcc
    14281 cgtcgtgccg ctcgtcgtga tgatcgccgc gtactcgtag ggccgaaagc gacgtccttc
    14341 gagaacgcgc gcgggctcgc ccggcgcaca ggaacgggcg agccacgact cgacgtcgat
    14401 cgcacccggc agcggcgatt cgccgatcga gagcagcctg aacgagggcg ccgccccggc
    14461 tcggtagacc tcggccagtt cgcgcgcgcg ctcggtattc ggcaacaccg cgccggccag
    14521 cggcagccgg ccggcaatcg caccgacttc ggcgcgcgag aacgtcggcg gcaccagcag
    14581 cgagatcacg cccgcttcct cggccgcgag ccgcagcagc atcagcgtgg ccgaattcgg
    14641 cgcctgcagc gcgaggacat cgtccttcgc gaacccgcag tgaacgagcc cgacggcgag
    14701 gcggtcgatc gcctgcgccg cggccttcca ggtgaatcgt cgcgtgccga acacgagcgc
    14761 gtcgcgctcg ggaaaccgca gcgcgttctc ctgccagaaa tccaccggat actggcgcgt
    14821 ccagtacccc tcctgctcgt accgggcgac cgtctccggc gtatagcgtg ccacgcgtgc
    14881 cacgagtgct cctcggtcgg gtcgctacac ttcgagcgcc agcctgaatg cctgtgaaac
    14941 gctctcgatc gcgtgcatcg cctgctccgg gtcgccgatg agctccactc tcggggcggc
    15001 cttcgatgcc gtcaactgct cgtacagccg cgtgtcggga ttcagcccgg tggcaaggaa
    15061 cacggtgtcg gcggggatgc ggatttcctc gttgccctgc tccacgacga cctcgctcgc
    15121 ggtgatctcc ttcgccaccg cgttcacgag gatctcgacg ccggcgatcg cgacctcctt
    15181 gccgagcacc cagcgcgtgg agccgcccac ggagccgccc aggcgccgct gccggctcac
    15241 cagcgtgacg tggtggccct tgcgatcgag gatctcgcga tcgttgtcgg cgagcgcctg
    15301 gtagtcgtcg aggaaatcga ggacttcggt gcgcacctgc cagcgccggg cgagaaacgg
    15361 cgcgatctcc acgccgatgc cgccgccgcc gatgacgacg acgcgctcgc cgatcgggat
    15421 gcggccctcc agcgctgcgg tcgccggatg cacgtgcggc aggtccgcac cgggcagcga
    15481 cgactcgacg ggctcgctgc ccggcgagac ggcgatgaga tcggcgttca tcgcgcgtag
    15541 cagatccgca gtcgcctcgg tctgcatgcg gaacgcgacg ccggccttcg cgcactgccc
    15601 gaccagccat tcgaggaagg tgaacagctg ctcgcgtccg ggcggcgtcg aggcgtgctg
    15661 ccacatgccg cccaggtgcc ccttgcgctc gatcagcgtg acctcgtgcc cgcgctcggc
    15721 gctggtcagc gcgaactgca gtccggccac cccgccaccg acgacgacga cgcgccggcg
    15781 aacgcgcgcc atggggatcg gctcgacttc cgaggccgcg ccctcgcgcg ggttcaccag
    15841 gcaacgcacg ggctcgttgc ggatgaccgc cgtgtccatg cactcgttgc aggcgatgca
    15901 gtagcggatg tcggaaaacc ggccctcgcg cgccttcagc ggccagtcgg cgtcggccag
    15961 cgactgccgt cccaggctga tcaggtcggc ccagccgtgg cgcaggatcc gctcggcctc
    16021 gagcggatgg ttgttgcggt tcgacgccgc gaccgccgcg gagcccacca cgcgcttgat
    16081 ctcgcgcgcg aggtacgcgt aggcgccctg cggaacgttc ggcgtgagct gcgggaccga
    16141 ggttgcgtgc ccgccgcccg tcacgttgaa gaagcagacg ccggcgcgct cgagctgcac
    16201 ggcgttgaac tgggcaccct cgatcccgta gcccccgggc aggaactcgc tgccgtgcag
    16261 ccggtagaag accggaaaat cctcgccgag cgccttcttg atgccgcgca ccgtctccac
    16321 cgcgaagcgg atgcgcttct cgggcgtgcc gccccacgcg tcgcagcggt cgttgaacac
    16381 gcgcgaggcg aacaggctgt gcccgcttcc ccccacgccg aggatgtcga cggccatgaa
    16441 cccggcccgg gcggccctca ccgaggccgc gacgacggcc tcgatcatct cctcgatctg
    16501 ctcggccgga atctcggcca ggctcggacg gtcggtggcc ttctcgcgat gcaggcggct
    16561 cacgtggaac aggcgccgga tgccgatctg cgcgtaggga accgtgccgt ggcggcgcaa
    16621 ctcctgcacg aggcgcgcat agcgctgcac gtgcaggtcg ttgtcgagga agagctcgtg
    16681 cgggctgccg gcgtggtcga cgaagaacgc gtcgatgacg cccacgccca tgaggccgaa
    16741 cccgcctttc gcgcgcgcca cgtagaaatc gatgccgcgg tcggtgatgc cctcctgcat
    16801 cgagccgagg ttcgcgtgca tcggcggcat cgtcagccgg ttgggcacgc gcagcccgcc
    16861 gatggtgatg ggctcgaaca ggagcggcgt cagcatgcgg tctccaatta cttgcgttct
    16921 gcaattattg cgtttcgcta gtgtatggcg tctagaatgt cgtgtcaacg ggaatcgatc
    16981 gagcgcaaat gcaagagaac gcgcagagca gccgccgcga cggcctccta ccgctcgagc
    17041 agcggttcac ctaccgcttc gcgctcgtgg cgagccggat ggggacggca ctggccccct
    17101 cgcacgagcg atacgggctc tcggtgggca cgtggcgcgt gctggcggtc atcgcgcgct
    17161 acgagccgct gtcggccaag gaactcgcgc agcggacctc gacggatccg tttcgagtga
    17221 cgcgggcgtt gacgatgctc gccgagaagg gattggtgag ccggcaggcc gatccgaagg
    17281 accgacgccg cgtgagcctg cgcctgacgc gcgccgggcg cggcgtgcac gacgagatcg
    17341 cggccacgct cagcgagatg gagcgctcgg tgctcgttgc gttgagcgcg cgcgagcagg
    17401 ccatgctgca tcggctgctg gacaagctcg atgtgcgcat cgctgcgtgc ctgcaggagc
    17461 agtcctggtc cgtgcagggc ccgccgcgat gacgagccgg ccggcgcgcc gcgcagcggc
    17521 gagacgcgcg cgcgcaaacg gtttaccctc tcgcctcggc tcgcgcgagc cggcgcgact
    17581 ccctccacga acgagaacat gaaacccagg attctccagt cggctcgcct gatgcccgcg
    17641 ctcgaagccc gccttcgcag cgaattcgaa gcccatccgc tgtggaacga gccggatccg
    17701 gccgcattcc tcgaagcccg cggaggcgag ttcgagatcc tggtcaccaa cgcgacggcg
    17761 ggcgcgagcg catcgatgat ggacgcactg ccggccttga aggcgatctg cagtctcggc
    17821 gtgggctgcg acgcgatcga cctgcaggct gcgcgcgagc gcggcgtggt cgtcgccaac
    17881 acgcccgacg tgctcaccga ctgcgtggcc gacatcgcga tgggcctgct catcgacgtc
    17941 gcccgcgggc tttcggccgc cgaccggcac gtgcgccgcg gcaagtggct cgaggggccg
    18001 ttcccgctga ccacgcgggt caccggaaaa aggctcggca ttctcggcct ggggcgcatc
    18061 gggcaggcgg tcgcgcggcg cgcgcgcggc ttcgagatgc aactgcgcta ccacagccgc
    18121 cggcccgtcg cctcgtcgaa cctcgtctac gaaccctcgc tcgtgcaact cgcgcaatgg
    18181 gccgattttc tcgtcgtcgc ctgctcgggc ggcgcggcga cgcaggggct cgtctcggaa
    18241 gccgtgctgc gcgcgctcgg cccgcagggc ttcctcgtca acgtcgcgcg cggcagcgtg
    18301 gtcgacgaag cggcgctcgt gcgcgcgctg cagcagggca cgatcgccgg cgcgggcctg
    18361 gacgtctacg ccgacgagcc gaacgtgccg cccgcgctga tcgcgctgga gaacgcggtg
    18421 ctgctgccgc acatggcgag cggcacgcgc gagacgcggc aggcgatggc cgatctcgtg
    18481 ttcgagaacg tgcagtcgtt cgtcgagcac ggcaggctga agacgccggt tccggcctga
    18541 ggcaagcgct gccgcacccg ccatgggctc cggcgcgcgg ccggcgcccg cgccgccgcg
    18601 acgccgctac ccgaggtagc gcggaagcag cgtggagatg accgggaacg cgatgaggat
    18661 tcccaggcgg aagacgtcgg ccacgacgaa cggcgcgacg ccgcggaaca tcgtgcggat
    18721 cgagacgtcg ggcagcagcc cgcgcaggac gatgacgttc atgcccaccg gcggcgtgat
    18781 gaagctgatc tccatcacga cgacgacgat gatcccgaac cagatgagat cgaagccgag
    18841 gtgctgcacg agcggataga acacgggcac cgtgagcagc agcatcgaca tgctctcgag
    18901 cacgcagccg agcacgatgt agagcgcaca gatcgcgagg atgacggtga tcgggctcag
    18961 gtcgaagcgc tgcacggcct gctgcaggtc gccgggcagc gtcgtgaagt tgacgaagtt
    19021 ggcgaagatc agcgcgccga tgaggatcat gaagagcatt cccgtggtgc gcgccgattc
    19081 cacgagcacc tcggagaact tgcgcggcgt gagctgcccg cgcgccagcg cgaagacgaa
    19141 tccgccgaag gcccccatgc cggccgcttc ggtggccgtg aagacgccgc cgtggggtct
    19201 cctcgttttc agtgcaataa gtgacggtac gaaaagctag cactgg
//
LOCUS       DAOQJH010000002        14015 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_2104_length_14015_cov_4.194556, whole genome shotgun sequence.
ACCESSION   DAOQJH010000002 DAOQJH010000000
VERSION     DAOQJH010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 14015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14015
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2104_length_14015_cov_4.194556"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..627)
                     /gene="ruvB"
                     /locus_tag="PLQ18_00100"
     CDS             complement(<1..627)
                     /gene="ruvB"
                     /locus_tag="PLQ18_00100"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012702191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HNE00075.1"
                     /translation="MIESDRLISAAASADDAAIDRAIRPKRLADYVGQQALREQLEIF
                     IHAARRRREALDHVLLFGPPGLGKTTLAHIIAAEMGVNLRSTSGPVLEKPGDLAALLT
                     NLEPYDLLFVDEIHRLSPVVEEVLYPAMEDYQIDILIGEGPAARSIKLDLPPFTLVGA
                     TTRAGLLTSPLRDRFGIVHRLEFYTTAELTQIVGRSAGILGVPTDDDGA"
     gene            1080..1322
                     /locus_tag="PLQ18_00105"
     CDS             1080..1322
                     /locus_tag="PLQ18_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HNE00076.1"
                     /translation="MDTPAAAETVLDLTGLLCPLPLLKTKQAMAKMESGERIRILATD
                     RSVVVDFRVFIDRFGHELLEFTESSGGHYVFVLRKA"
     gene            complement(1340..1954)
                     /gene="ruvA"
                     /locus_tag="PLQ18_00110"
     CDS             complement(1340..1954)
                     /gene="ruvA"
                     /locus_tag="PLQ18_00110"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008931032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HNE00077.1"
                     /translation="MIGRLRGRLILRQPPLLMIELGNGLAYELEASLSTCCALPETGA
                     EVVLYTHLAVREDAQVLYGFGTLAERTLFRNLIRVTGIGAKLALVILSGMSVDAFARC
                     LEAGDVATLIRLPGIGRKTAERLVVEMRDRIQSLELGAEFAPRLPARAPDAPPDPVQD
                     AEAALVALGYKPADATRMVRAVAADGLPSEELIRRALAATLSRG"
     gene            complement(1951..2451)
                     /gene="ruvC"
                     /locus_tag="PLQ18_00115"
     CDS             complement(1951..2451)
                     /gene="ruvC"
                     /locus_tag="PLQ18_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010325511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HNE00078.1"
                     /translation="MRILGVDPGSRLTGYGVIDVHGPRAVYVDSGCIRTPAGALGERL
                     CAIHRGLAEILRVHAPAAAAIEQVFLHRNAVSALKLGHARGVALLALAEAGLAVAEYM
                     PSVVKLAVVGTGRADKEQVQHMVTALLKLPRAPQADAADALAVAICHGTQLFAQARLA
                     GAGQGR"
     gene            complement(2469..3212)
                     /locus_tag="PLQ18_00120"
     CDS             complement(2469..3212)
                     /locus_tag="PLQ18_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018123083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNE00079.1"
                     /translation="MAGHSKWANIQHRKGAQDAKRGKLFTRFIREITIAARSGLPDPA
                     ANPRLRLAVDKALGANMSKDTIERAIKRGAGADDGSTLEEIRYEGYGPGGIAVMVDCM
                     TDNRNRTVAEVRHAFSKCGGNLGTSGSVAFQFTETGVISYPAGTDQEKVMEVALEAGA
                     DDLVENEDGSFDVLTAPDAFAAVREALDGAGLVAESAEVTMRAGNTVALGLDDARSMI
                     KLLDMLEDLDDTQNVYSNADIPEAVMAQL"
     gene            complement(3438..3869)
                     /gene="pedF"
                     /locus_tag="PLQ18_00125"
     CDS             complement(3438..3869)
                     /gene="pedF"
                     /locus_tag="PLQ18_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-550 PedF"
                     /protein_id="HNE00080.1"
                     /translation="MMKITMRAGLVLATLLTAATAHAHGDVTPQAVDTTGLDPLGDEN
                     WRESNPYRGNELAIKIGKSAFAQNCARCHGIDAISGGIAPDLRKLEKGALGDEWFQER
                     VRNGAVRNGAVYMPKFAKPEGALSQEALWAIRSWLDTVAID"
     gene            4244..6304
                     /locus_tag="PLQ18_00130"
     CDS             4244..6304
                     /locus_tag="PLQ18_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007990585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HNE00081.1"
                     /translation="MLDRLASQHAARVLSVARDGDARAGRSAPDAAISRSWERCIANH
                     GLEPILPRGTLVLDRPALAQRQEKLQDFVELARVEMDRLYQQIAGSGSAIILTDADGV
                     ILNCVTSPGLARMFQRAGLWLGADWSERHEGTNGIGTCIMERQPVTVHRDEHFLARHI
                     GLSCSAAPIFDPAGNLLAVLDASTVNSRDSKQSQFHALALAAMSAKTIENCAFQRHFR
                     QASVLRFHARPEYIGVIAEGLLAFDGDGRVLGANQSALNQLGGLSREELMHLRVADIF
                     DLSLDVLMGRASVHATVWPLLDRRGHGYYAMLRGPESALERGAMSMPAARRSTAAARP
                     AIPPQTAPAPGRTMSAVIAPRMDLEGLAGEDPAMAYNVRCARKVVDRNVTILLNGETG
                     TGKEAFAKAVHDASDRADKPFVAINCASIPENLIESELFGYKAGAFTGARREGMRGKI
                     VQAHGGTLFLDEIGDMPAHLQTRLLRVLEEREVVPLGGEAPIMVDLHVISATHRNLPD
                     LVASGSFREDLYYRLNGLVLTLPALRERADLAIVIERVLAAEAEGQPVELSFEARNLL
                     LEYPWPGNIRQLRNILRTAVALCEDAEIRPEDLPAELGRAPGMAGPGAAEAAPRLVSP
                     PVETIAATDPEGSLASAEKAALLRELERHRWNMTNTARDLGLSRNTLYRKLKKHGITP
                     PTLS"
     gene            6918..6992
                     /gene="pqqA"
                     /locus_tag="PLQ18_00135"
     CDS             6918..6992
                     /gene="pqqA"
                     /locus_tag="PLQ18_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013440845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone precursor peptide PqqA"
                     /protein_id="HNE00082.1"
                     /translation="MQWSKPEFTEMRFGFEVTMYIANR"
     gene            7142..8053
                     /gene="pqqB"
                     /locus_tag="PLQ18_00140"
     CDS             7142..8053
                     /gene="pqqB"
                     /locus_tag="PLQ18_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002814312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis protein
                     PqqB"
                     /protein_id="HNE00083.1"
                     /translation="MHIHVLGAGAGGGFPQWNCNCANCAGYRAGTLRATARTQSSIAV
                     SADGTDWILFNASPDIRAQLAAFAPMQPNRGIRDTGICAVLLIDSQIDHATGLLMLRE
                     HTRRLPVYLSAPAQQDLSTGLPLLKVLEHYCGTEVHELPLDGAAFTIPGAAGLVIRTV
                     ALASKAPPYSPHRHDPHPGDNVGVFIEDTRSGGRLFYAPGLGEIEDHLLPYLEQADLL
                     LVDGTFWTEDEMIRHGIVHKFAREMGHLPQSGEDGMIRALAPYRGRKVLIHINNTNPI
                     LVEDGPERAELAAHGIEVAYDGMDFEL"
     gene            8050..8793
                     /gene="pqqC"
                     /locus_tag="PLQ18_00145"
     CDS             8050..8793
                     /gene="pqqC"
                     /locus_tag="PLQ18_00145"
                     /EC_number="1.3.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013034384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline-quinone synthase PqqC"
                     /protein_id="HNE00084.1"
                     /translation="MSTQAPAWSREEFEARLRGLGSLYHIHHPYHVRMHEGQLSREQI
                     QGWVANRYYYQISIPRKDAAILANCPDRDTRRRWIQRIVDHDGNDAQEGGIEAWTRLG
                     IACGLDRETIESQRLVLPGVRFAVDAYVNFARSASWQEAACSSLTELFAPTIHRQRLN
                     HWPDRYPWIEPEGLQYFRNRLTEAHRDVQHGLAITLEHFASRLQQERALEILRFKLEV
                     LWTMCDAMWMAYVAELPPFTGCEAPAVPA"
     gene            8790..9053
                     /gene="pqqD"
                     /locus_tag="PLQ18_00150"
     CDS             8790..9053
                     /gene="pqqD"
                     /locus_tag="PLQ18_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002708785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis peptide
                     chaperone PqqD"
                     /protein_id="HNE00085.1"
                     /translation="MSGARLRIAPGYRLQWEPAQSCHVLLYPEGMVQLNPSAAAVLEL
                     CDGSLDADGIVAALEAKYPGAELAADVRQFLEVADERGWLRSA"
     gene            9025..10161
                     /gene="pqqE"
                     /locus_tag="PLQ18_00155"
     CDS             9025..10161
                     /gene="pqqE"
                     /locus_tag="PLQ18_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013219567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018149 - peptide cross-linking [Evidence
                     IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis protein
                     PqqE"
                     /protein_id="HNE00086.1"
                     /translation="MSAAGYVPPEVPSRPLWLLAELTYACPLQCPYCSNPLDYTAVRE
                     ELDTETWKRVLREARAMGAAQLGLSGGEPATRTDLEEIVAEARRLGYYTNLITSGLGL
                     DATRIERLHAAGLDHIQVSFQASTEELNNHLAGTDAFRHKLEIARAVKAAGYPMVLCF
                     VLHRDNTDSMDAILTLAAQLGADYVELATTQYYGWAWVNRDRLLPTREQLVRAEAVAH
                     RWQADMKGRMKIYYVVPDYFEDRPKACMNGWGKVFLTVAPDGTALPCHSARELPGLSF
                     PNVRDHSVEWIWKDSPGFNRFRGYGWMKEPCRSCDEKEKDFGGCRCQAFLLTGDPENA
                     DPVCSKSPEHGRVLAAIEQAAATPSERPLVFRNPRSVRRLHGEA"
     gene            10350..10811
                     /locus_tag="PLQ18_00160"
     CDS             10350..10811
                     /locus_tag="PLQ18_00160"
                     /inference="COORDINATES: protein motif:HMM:NF033749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohemerythrin"
                     /protein_id="HNE00087.1"
                     /translation="MSIPNYIEWSDELSVGIQEIDEQHKYLVQLVNELNVHLLSHGSA
                     KETVHEALDKLIRYTETHFVVEESLMRILGYPDYDSHRQHHEDLIQMVLDFRQQLNVE
                     GRVSRTELMEFLHKWLTYHILKEDKQYSEHFLSRGAQRSWLKGSWLKKFWK"
     assembly_gap    10815..10914
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(11060..12100)
                     /gene="pip"
                     /locus_tag="PLQ18_00165"
     CDS             complement(11060..12100)
                     /gene="pip"
                     /locus_tag="PLQ18_00165"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HNE00088.1"
                     /translation="MDSCLFPPCEPYAVHALAVGEGHVLHVEECGTPDGLPVIFLHGG
                     PGSGCNPLQRRLFDPARWRIVLVDQRGAGRSLPAGCIEHNSTADLLGDLERIRFALGI
                     DRWVVAGGSWGATLGLLYAEAFPDSTLGLLLRGTFLARPRDIAWYYRADGVALRFPSA
                     WRAFVGHLDETERRDLLTSYYRRLTSSDPEVVARAATAWAGWDNAVATWTLPPDREPQ
                     LPPDPLRRIAQARIAAHYLHHACFIVPNRVLANAHRLDGIPGILLHGELDRVCPIVQA
                     EALGAVWPDAELVRLPKAGHLLAEPPMLAAWVEAAARLYEAVAPAADRGRPASLFTCF
                     ADVGEQVSIDAG"
     gene            complement(12215..12679)
                     /locus_tag="PLQ18_00170"
     CDS             complement(12215..12679)
                     /locus_tag="PLQ18_00170"
                     /inference="COORDINATES: protein motif:HMM:NF028509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HNE00089.1"
                     /translation="MKFIRILTALFTTALLAFSSTSAHAWATEKDALALLEQAVAEIK
                     DKGVDAALAEFNNPKGKFVKGELYVFAFDMEGTYKANGQNPALVGKPARDLVDVAGNP
                     VVATMLDIAKNKGEGEVEYMWLNPKVNKLQRKMSHIKVVNGLIVGVGHYFVN"
     gene            complement(12960..13769)
                     /locus_tag="PLQ18_00175"
     CDS             complement(12960..13769)
                     /locus_tag="PLQ18_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNE00090.1"
                     /translation="MTPAHALLALNAVAGREVAKFCRQYGRLFSSLVRPSLWLIVFAA
                     GFQNVFGVAIIPPYDTYIEYRVYIAPGLLGMVLLFNGMQSSLAMVYDREMGMMRLLLT
                     APLPRWWLLFCKLAAGTLLSVLQAYAFLLVAAAFGVEIPAGGWLAVLPALAAGGLMLG
                     ALGLLLSVYVKQLENFAGTMNFVIFPMFFISSALYPLWKLRESGAEIVWHIARWNPFT
                     HAVELIRYALYGRFEPLACTVVLGSAALFFIAATLGYDPQRGLLKRSGRPG"
BASE COUNT         2275 a         4958 c         4657 g         2025 t
ORIGIN      
        1 ggcgccgtcg tcatcggtcg gcacgccgag gatgccggcc gagcggccga cgatctgcgt
       61 gagttccgcg gtcgtgtaga actccagccg gtgcacgatc ccgaagcggt cacgcagcgg
      121 gctggtcagc aggccggcgc gcgtggtcgc gccgaccagc gtgaacggcg gcaggtcgag
      181 cttgatcgag cgggcggccg ggccctcgcc gatcaggatg tcgatctggt agtcctccat
      241 cgccggatac aggacttcct cgaccaccgg cgacaggcgg tggatctcgt cgacgaacag
      301 cagatcgtag ggttcgaggt tggtcagcag cgccgcgagg tcaccgggct tctccagcac
      361 cggtccggag gtgctgcgca ggttcacgcc catctcggcg gcgatgatgt gcgcgagcgt
      421 ggtcttgccg agtccgggcg ggccgaacag cagcacgtga tcgagcgcct cgcgccggcg
      481 gcgcgcggca tggatgaaga tttcgagctg ctcgcgcagc gcctgctggc cgacgtagtc
      541 ggcgagccgc ttcgggcgga tggcgcggtc gatcgccgca tcgtcggcac tggcagccgc
      601 ggagatcagg cggtcggatt cgatcatgac ggattcgggg cactcggcag ccggacggac
      661 acgggtccgg atcaagatgg cgcaaaacgt cgacttgcgt cgcgccgtcc atcgtaagag
      721 caagagccgg gccacgcacc gctgctgcca tgcgtcatcg acccggaagg cgcggacacg
      781 gcccgtgcgc ccgcgaagac gctgaatccc tgaaatccgt ccggctggca agccagtctc
      841 acagcgtgac cgcgtggaaa cgatgacggc tggatgaaac gcatcgcgaa agcaggcgct
      901 gcagcgcgca ggccggcggt gtggacgacc acgcgcaccg cccgccctgc ttccgccgtc
      961 attcgcatcg aggccgcccg ctgccggcgc cggtggcggc agggttctcc cggtctgcgc
     1021 atccgggctt cgctgtcacc ggtagcgcgc gccggccgtt gtgcggacaa tgcactgcca
     1081 tggatacgcc tgccgccgcc gaaaccgtac tcgatctcac cggactgctc tgtccgctgc
     1141 cgctgctgaa gaccaagcag gcgatggcga agatggagtc cggcgagcgc atccgcatcc
     1201 tcgcgaccga ccgcagcgtc gtcgtcgatt tccgcgtgtt catcgaccgc ttcggtcacg
     1261 aactgctgga attcaccgaa tcatccgggg gccactacgt gttcgtgctg cgcaaggcct
     1321 gagcactgcc cagaaacgct cagccgcgtg acagcgtcgc cgccagcgcc cggcggatca
     1381 gctcctcgct cggcaggccg tcggcggcca cggcccggac catgcgcgtc gcatcggccg
     1441 gcttgtagcc gagcgcgacc agcgccgcct cggcatcctg caccgggtcg ggaggtgcgt
     1501 ccggcgcgcg cgccggcagc cgcggcgcga actcggcgcc gagttccagc gactggatgc
     1561 ggtcgcgcat ctccaccacc agccgctcgg cggtcttgcg gccgatgccg ggcaggcgga
     1621 tcagcgtcgc cacgtcgccg gcttccaggc agcgcgcgaa ggcatcgacg ctcatgccgg
     1681 agaggatcac cagcgcgagc ttcgcgccga tgccggtgac cctgatcagg ttgcggaaca
     1741 gcgtgcgctc ggcgagcgtc ccgaagccgt agagcacctg tgcgtcctcg cgcacggcca
     1801 gatgcgtgta cagcacgacc tcggcgccgg tctcgggcag cgcgcagcag gtcgacagcg
     1861 aggcttcgag ctcgtaggcg aggccgttgc cgagttcgat catcagcagc ggcggctgcc
     1921 tgaggatcag ccggccgcgc agccggccga tcatcgcccc tgccccgcgc ccgcgagccg
     1981 cgcctgcgcg aacagctgcg tgccgtgaca gatcgcgacg gccagcgcgt cggcggcatc
     2041 ggcctgcggc gcacgcggca gtttcagcag tgcggtgacc atgtgctgca cctgttcctt
     2101 gtcggcgcgg ccggtgccga ccacggcgag cttgacgacc gagggcatgt attcggcgac
     2161 cgcgagcccg gcctcggcga gcgcgagcag cgccacgccg cgcgcatgac cgagcttcag
     2221 ggccgagacg gcgttgcggt gcaggaacac ctgctcgatg gcggcggccg ccggcgcatg
     2281 cacgcgcagg atttcggcga gtccgcggtg gatcgcgcac agccgctcgc cgagcgcgcc
     2341 ggccggcgtg cggatgcagc cgctgtcgac gtagacggcg cgcggaccgt ggacatcgat
     2401 gacgccgtag ccggtcagcc gcgagccggg atcgacgccg aggatgcgca tggcgccgga
     2461 aaccggcttc agagctgcgc catcaccgct tcggggatgt cggcgttcga atagacgttc
     2521 tgcgtgtcgt cgaggtcttc gagcatgtcg agcagcttga tcatgctgcg cgcatcgtcg
     2581 aggccgagcg cgacggtgtt gccggcgcgc atcgtgacct cggcgctctc ggccacgagt
     2641 ccggcaccgt cgagcgcttc ccggacggcc gcgaaggcat cgggcgcggt cagcacgtcg
     2701 aaggagccat cctcgttctc gacgaggtca tcggcaccgg cctcgagcgc gacctccatg
     2761 accttctcct gatcggtgcc ggccgggtag ctgatgacgc cggtctcggt gaactggaag
     2821 gccaccgagc ccgaggtgcc gaggttgccg ccgcacttcg agaaggcgtg gcgcacttcg
     2881 gcgaccgtgc ggttgcggtt gtcggtcatg cagtcgacca tcaccgcgat gccgcccggg
     2941 ccgtagccct cgtagcggat ctcctccagc gtcgagccgt catcggcacc ggcaccgcgc
     3001 ttgatcgcgc gctcgatggt gtccttggac atgttggcgc cgagcgcctt gtcgacggcc
     3061 aggcgcagcc gcgggttggc ggccgggtcc ggcagcccgc tgcgggcggc gatggtgatc
     3121 tcgcggatga agcgggtgaa cagcttgccg cgcttggcgt cctgcgcgcc cttgcggtgc
     3181 tggatgttcg cccatttgct gtggccggcc atgcgctgcg tctccagaag atacggtgtg
     3241 ccgtgccggc ggcggtgccg ggacggccgg aaaaatcggg ggcgcgagtc taacaccgcc
     3301 ctccgggcgc ccggaaatcc gcacgccgga gccctaaaga cagcgccccc gcccggcgga
     3361 tcggccggac gggggcgctg gcggctcggg gcgcgcgggc atccgccgcg cccgccccca
     3421 aggctttcag ccgtggttca gtcgatcgcg accgtgtcga gccaggagcg gatcgcccac
     3481 agcgcttcct gcgacagcgc accttcgggc ttcgcgaact tcggcatgta gacggcgccg
     3541 ttgcgcacgg cgccgttgcg cacgcgctcc tggaaccatt cgtcgccgag cgcgcccttt
     3601 tccagcttgc gcaggtcggg ggcgatgccg cccgagatcg cgtcgatgcc atggcagcgc
     3661 gcgcagttct gcgcgaacgc cgacttgccg atcttgatcg ccagttcgtt gccgcggtac
     3721 ggattgctct cgcgccagtt ctcgtcgccg agcgggtcca gcccggtggt gtcgaccgcc
     3781 tgcggggtca cgtcgccgtg ggcgtgggcg gtggccgccg tcagcagggt cgccagcacc
     3841 agacctgcac gcatcgtgat cttcatcatc gccaagctct cctccgggtc ttatgggatg
     3901 agggggtgtt cgaacgccac gcaattcagc aatgagcgtg ccatgagcaa aatgcaaagg
     3961 ccaaatcatt gatccagagc atttttgcgc acggacaaca cgttcagccc ctgcaaatgg
     4021 cccggccatc ccggccggac ggcgatggca catggccggc tgtgacagtt ccggtacgca
     4081 ctgtcacggc tggtcacggc tgttccgcac accggcaagc atttcgcagg gcggcagaaa
     4141 acgggtttag aatcaggttt taccgcgcgt ggcgtaatgc cgtgcccggc gatacgccca
     4201 caacgacaag gccgtgcccc ccggcgcggc ggaggaggag agcatgttgg accgccttgc
     4261 gtcgcagcac gccgctcgcg tgctgtcggt tgcccgggac ggcgatgccc gtgctggccg
     4321 ctcggccccc gacgcggcga tctcgcgctc gtgggagcgc tgcatcgcca accatggcct
     4381 cgaaccgatc ctgccgcgcg gcacgctggt gctcgaccgg ccggcccttg cgcagcgcca
     4441 ggagaaactg caggacttcg tcgagctggc gcgcgtcgag atggaccgcc tgtaccagca
     4501 gatcgccggc tccggcagcg cgatcatcct gaccgatgcc gacggcgtga tcctcaactg
     4561 cgtgacctcg ccgggcctgg cgcgcatgtt ccagcgcgcc ggcctctggc tcggcgccga
     4621 ctggagcgag cgccacgagg gcaccaacgg catcggcacc tgcatcatgg aacgccagcc
     4681 ggtgaccgtg caccgcgacg agcacttcct cgcccgccac atcggcctga gctgctcggc
     4741 cgccccgatc ttcgatccgg ccggcaacct gctcgccgtg ctcgatgcgt ccacggtcaa
     4801 ttcgcgtgac tccaaacaga gccagttcca cgcgctggcg ctggcggcga tgtcggccaa
     4861 gaccatcgag aactgcgcgt tccagcgcca cttccgccag gcctcggtgc tgcgcttcca
     4921 cgcccggccc gagtacatcg gcgtcatcgc cgagggcctg ctcgccttcg acggcgacgg
     4981 tcgcgtgctc ggcgccaacc agagcgcgct gaaccagctc ggcggcctgt cgcgcgaaga
     5041 actcatgcac ctgcgcgtcg ccgacatctt cgacctgagc ctcgacgtgc tgatgggacg
     5101 cgccagcgtg cacgccaccg tctggccgct gctcgaccgc cgcggtcacg gctattacgc
     5161 gatgctgcgc ggccccgaga gcgccctcga acgcggggcg atgtcgatgc ccgccgcacg
     5221 ccgcagcacc gcggcggcac gcccggcaat ccccccgcag accgcgcccg cccccgggcg
     5281 cacgatgagc gcggtgatcg cgccgcgcat ggacctcgaa ggcctcgccg gcgaggaccc
     5341 ggccatggcc tacaacgtgc gctgcgcgcg caaggtcgtc gatcgcaacg tcacgatcct
     5401 gctcaacggc gagaccggca ccggcaagga ggcctttgcc aaggccgtgc acgatgccag
     5461 cgaccgcgcc gacaagccct tcgtcgcgat caactgcgcg tcgatcccgg aaaacctgat
     5521 cgaatccgaa ctgttcggct acaaggccgg cgccttcacc ggcgcgcgcc gcgaaggcat
     5581 gcgcggcaag atcgtgcagg cgcacggcgg cacgctgttc ctcgacgaga tcggcgacat
     5641 gccggcgcac ctgcagacgc ggctgctgcg cgtgctggag gagcgcgagg tcgtgccgct
     5701 cggcggcgag gcaccgatca tggtcgacct gcacgtgatc agcgccacgc accgcaacct
     5761 gccggatctc gttgccagcg gcagcttccg cgaggacctc tactaccgcc tcaacggtct
     5821 ggtgctgacg ctgccggcgc tgcgcgagcg cgccgatctc gccatcgtca tcgagcgcgt
     5881 gctcgccgcc gaggccgagg gccagccggt cgagctgtcc ttcgaggcgc gcaacctgct
     5941 gctcgaatac ccttggcccg gcaacatccg ccagctgcgc aacatcctgc gcacggccgt
     6001 cgcgctgtgc gaggacgccg agatccgccc cgaggacctg cccgccgaac tcggccgcgc
     6061 tccgggcatg gccggccccg gcgccgccga ggcggcgccg cgcctcgtgt cgccgccggt
     6121 cgagacgatc gccgcgaccg atccggaagg ttcgctggcg agtgccgaga aggccgcgct
     6181 gctgcgcgag ctggaacgcc accgctggaa catgaccaac accgcgcgcg atctcggact
     6241 gtcgcgcaac acgctctatc gcaagctcaa gaagcacggc atcacgccgc cgacgctgtc
     6301 ctgagcagtc ccgcccagac ctgttgctgt accggcgtcc cggattcgaa caccggccgg
     6361 gacaccgtcg catcgttccc gggacaccac ccccgccccc ggccgcggcg ggccgctggc
     6421 gacagccgca aacccgcatt ccgggccgct ccccgctggc tggcatgcac attgctgata
     6481 ctcaaactgc gagttctgac gccgtgccgg caccgtcccc ccctttccgg tgtcggccga
     6541 cctccagcgc cggggctcgc actcctcctc gcttgacccc cgcgatccgg gactcttggg
     6601 gcgaccgaac gacacggtcg cccgtttttt tgcgcgtctt cccacgcccg cctcccggca
     6661 ccgcccgcag ggcgccggcg tgcctcgact gacacactct gtcccgccgg ccggcgcttc
     6721 tgtccgcacg gacggcccgg atgctgcgag cgcacaacga caaacgtcgc atgattcgaa
     6781 ataaaccttt gaccttccat gcatttttga tcgggcttcc gcggctgacc ggctggcatg
     6841 cgacgtgcta aaactgccgg gcggcagtcg ccggacccag aaccccaatc acgaccccga
     6901 tcaccgagga gatcaccatg cagtggagca agcccgagtt caccgagatg cgtttcggct
     6961 tcgaagtgac gatgtacatc gccaatcgct gagctgatcc gcccgacgcc ggcccgggcc
     7021 accttccggg ccggcgtttt cattcggccc aggaccggca gtgccgctgc cgcccggcca
     7081 tccctctccc gagagtgcgg cccaccgcac cgccctgccc gaccacccac gggacaccga
     7141 catgcacatc cacgtcctcg gcgccggcgc cggcggcggt ttcccgcagt ggaactgcaa
     7201 ctgcgcgaac tgcgccggct accgtgccgg cacgctgcgg gcgacggcac gcacgcagtc
     7261 ctcgatcgcg gtcagtgccg acggcacgga ctggatcctg ttcaacgcct cgcccgacat
     7321 ccgcgcccag ctcgcggcct tcgcgccgat gcagccgaac cgcggcatcc gtgacaccgg
     7381 catctgcgcg gtcctgctga tcgacagcca gatcgaccac gccaccggcc tgctgatgct
     7441 gcgtgaacac acgcgccggc tgccggtgta cctgagcgcc ccggcgcagc aggacctctc
     7501 caccggcctg ccgctgctga aggtgctcga acactactgc ggcaccgaag tgcacgaact
     7561 gccgctcgat ggcgcggcgt tcacgattcc cggtgccgca gggctcgtga tccgcaccgt
     7621 cgcgctggcg agcaaggcgc cgccgtactc gccgcaccgc catgacccgc atcccggtga
     7681 caacgtcggt gtgttcatcg aggacacccg cagcggcggc aggcttttct acgcgcccgg
     7741 cctcggcgaa atcgaagacc acctcctgcc ctacctcgaa caggccgacc tgctgctggt
     7801 cgacggcacc ttctggaccg aggacgagat gatccgccac ggcatcgtgc acaagttcgc
     7861 gcgcgagatg ggccatctgc cgcagtccgg cgaggacggc atgatccggg cgctggcgcc
     7921 gtatcgcggc cgcaaggtgc tgatccacat caacaacacc aacccgatcc tcgtcgagga
     7981 cgggcccgag cgcgccgaac tggccgcgca cggcatcgaa gtggcttacg acgggatgga
     8041 tttcgaacta tgagcacgca agcgccggcc tggagccgtg aggaattcga ggcgcgcctg
     8101 cgcggcctcg gcagcctgta ccacatccac cacccctacc acgtgcgcat gcacgagggg
     8161 cagctgagcc gcgagcagat ccagggctgg gtcgcgaacc gctactacta ccagatcagc
     8221 atcccgcgca aggatgcggc gatcctcgcc aactgcccgg accgcgacac gcgccggcgc
     8281 tggatccagc gcatcgtcga tcacgacggc aacgacgcgc aggaaggcgg catcgaggcc
     8341 tggacgcggc tcggcatcgc ctgcggcctc gatcgcgaga ccatcgagtc gcagcgcctg
     8401 gtgctgccgg gcgtgcgctt cgcggtcgat gcctacgtga acttcgcgcg ttcggcgagc
     8461 tggcaggagg ccgcctgctc gtcgctgacc gaactgttcg cgccgaccat ccaccgccag
     8521 cgcctgaacc actggccgga ccgctacccg tggatcgagc ccgagggcct gcagtacttc
     8581 cgcaaccgcc tgaccgaggc gcaccgcgac gtgcagcacg gcctcgcgat cacgctggag
     8641 cacttcgcga gccgcctcca gcaggaacgc gcgctcgaga tcctgcgctt caagctcgaa
     8701 gtgctgtgga cgatgtgcga tgcgatgtgg atggcctacg tcgccgagct gccgccgttc
     8761 accggctgcg aagccccggc ggttccggca tgagcggcgc gcgccttcgc atcgcgcccg
     8821 gctaccgcct gcagtgggag ccggcgcaaa gctgtcacgt gctgctgtat ccggaaggca
     8881 tggtgcagct caacccttcc gccgctgccg tgctcgaact ctgtgacggc agcctcgatg
     8941 ccgacggcat cgtcgcggcc ctcgaagcga agtaccccgg cgccgagctc gccgccgacg
     9001 tgcgccagtt cctggaggtc gccgatgagc gcggctggtt acgttccgcc tgaagtaccg
     9061 tcacggccgc tgtggctgct cgccgagctg acctacgcct gcccgctgca gtgcccgtac
     9121 tgctcgaatc cgctcgacta cacggccgtg cgcgaggagc tcgacaccga gacctggaag
     9181 cgcgtgctgc gcgaggcccg ggcgatgggg gcggcccagc tcgggctttc cggcggcgag
     9241 ccggcgacgc gcaccgacct cgaggagatc gtcgccgagg cgcgccggct cggttactac
     9301 acgaacctga tcacctccgg actcggcctc gacgcgacac gcatcgagcg cctgcacgcg
     9361 gccggactcg atcacatcca ggtgagcttc caggcgagca ccgaggagct caacaaccac
     9421 ctcgccggca ccgatgcctt ccggcacaag ctggagatcg cgcgcgcggt caaggccgcc
     9481 ggctacccga tggtgctctg cttcgtgctg caccgcgaca acaccgactc gatggacgcg
     9541 atcctgacgc tggcggcaca gctcggtgcc gactacgtcg agctcgcgac cacgcagtac
     9601 tacggctggg cctgggtcaa ccgcgaccgg ctgctgccga cgcgcgagca gctcgtgcgc
     9661 gccgaagcgg tcgcgcaccg ctggcaggcc gacatgaagg gccgcatgaa gatctactac
     9721 gtcgtgcccg actacttcga ggaccggccg aaggcctgca tgaacggctg gggcaaggtg
     9781 ttcctgaccg tcgcccccga tggcaccgcg ctgccctgcc actcggcgcg cgagctgccg
     9841 ggcctgagct tcccgaacgt gcgcgatcac tcggtcgaat ggatctggaa ggactccccc
     9901 ggcttcaacc gcttccgcgg ctacggctgg atgaaggaac cctgccgctc ctgcgacgag
     9961 aaggaaaagg acttcggcgg ctgccgctgc caggccttcc tgctgaccgg cgaccccgag
    10021 aacgccgatc cggtctgcag caagtcgccc gagcacggcc gcgtgctggc cgcgatcgaa
    10081 caggccgcgg cgacgccgtc cgaacggccg ctggtgttcc gcaatccgcg cagcgtccgt
    10141 cgcctgcacg gcgaagcctg agcgtccggc gccctcgcca ctggcgaaaa ataaactgaa
    10201 tgtaatactc ggtgtccgac ggatcagccg ccccggggcg gctgacctgc atcagcccgc
    10261 cgaggtccgg tgccggctac gtttcgcatc ttccgaaccg cgcgccgcgg caccggacca
    10321 tcaagcgacc accggagaga gtcggccaca tgagcattcc gaattacatc gaatggagcg
    10381 acgagctttc cgtcggcatc caggagatcg acgagcagca caagtatctg gtgcagctgg
    10441 tcaatgagct gaacgtgcac ctgctgagcc acggctccgc gaaggagacc gtgcacgaag
    10501 ccctggacaa gctgatccgc tacaccgaga cgcacttcgt ggtcgaggaa agcctgatgc
    10561 gcatcctcgg ctatcccgac tacgactcgc accgccagca ccacgaggac ctgatccaga
    10621 tggtgctcga cttccgccag cagctcaacg tggaaggtcg cgtctcgcgc accgaactca
    10681 tggagttcct gcacaagtgg ctgacctatc acatcctgaa ggaagacaag cagtactcgg
    10741 agcacttcct gagccgtggc gcgcagcgct cgtggctgaa gggctcgtgg ctgaagaagt
    10801 tctggaagtg aagcnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncgcgca
    10921 gcgctcgtgg ctgaagggct cgtggctgaa gaagttctgg aagtgaagct ccgcaactga
    10981 agcccggaag agagcccgca ccgggcgcgc tgaccggaaa ccgctgaaca cgacgccggc
    11041 ctgccggcgt cgctgcgttt cagccggcgt cgatgctgac ctgctcgccg acatcggcga
    11101 agcaggtgaa cagcgaggcc ggccggccgc gatcagccgc cggtgccacg gcctcgtaca
    11161 gccgtgcggc cgcctcgacc caggccgcca gcatcggcgg ctcggcgagc agatgcccgg
    11221 ccttcggcag gcgcaccagc tccgcatccg gccacaccgc cccgagcgcc tcggcctgca
    11281 cgatcgggca gacgcgatcg agctcgccgt gcagcaggat gccggggatg ccgtcgagac
    11341 gatgggcgtt ggcgagcacc cggttcggca cgatgaagca ggcgtgatgc aggtagtgcg
    11401 cggcgatgcg cgcctgcgcg atgcgccgca gcggatcggg tggcagctgc ggctcgcgat
    11461 cgggcggcag cgtccaggtc gcgacagcgt tgtcccagcc cgcccaggcg gtggcggccc
    11521 gcgcgacgac ctccgggtcc gacgaggtca ggcgccggta gtaactggtc agcaggtcac
    11581 ggcgctcggt ctcgtcgaga tggccgacga aggcccgcca ggcgctcgga aagcgcagcg
    11641 ccacgccatc ggcgcggtaa taccaggcga tgtcgcgcgg gcgcgcgagg aaggtgccgc
    11701 gcagcagcag cccgagcgtg ctgtccggaa aggcctcggc gtacagcagg ccgagcgtcg
    11761 ccccccagga gccgccggcg acgacccagc ggtcgatgcc gagcgcaaag cggatgcgtt
    11821 cgagatcgcc gagcaggtcg gcggtcgaat tgtgttcgat gcagccggcc ggcagcgaac
    11881 gcccggcccc gcgctgatcg accagcacga tgcgccagcg cgccggatcg aacagccgcc
    11941 gctgcagcgg attgcagccc gaacccgggc caccgtgcag gaagatcacc ggcagcccgt
    12001 cgggcgtgcc gcattcctcc acgtgcagca catggccctc gccgacggcg agggcatgca
    12061 ccgcgtacgg ctcgcagggc ggaaacaggc agctgtccaa ggttcgccct ccggccgcac
    12121 gccggccgca gccggccggc gtgtgcctgc agaaagtccc gtcgtgacga cgcagcggcg
    12181 ggaaggccgc tccgctcacg gatcagttca cggctcagtt cacgaaatag tgaccgacgc
    12241 cgacgatcag gccgttgacg accttgatgt gcgacatctt gcgctgcagc ttgttgacct
    12301 tcgggttcag ccacatgtac tcgacctcgc cctcgccctt gttcttggcg atgtcgagca
    12361 tggtcgcgac caccgggttg ccggcgacat cgacgaggtc acgggccggc ttgccgacca
    12421 gagcggggtt ctggccgttg gccttgtagg tgccctccat gtcgaacgcg aacacgtaca
    12481 gctcgccctt cacgaacttg cccttcggat tgttgaactc ggcgagcgcg gcatcgacac
    12541 ccttgtcctt gatctcggcg acggcctgtt ccagcagcgc cagcgcgtcc ttctcggtcg
    12601 cccaggcgtg ggcgctggtc gagctgaagg ccagcagcgc ggtcgtgaac agggccgtca
    12661 ggatcctgat gaatttcatg gagctttctc ctccggtctg aagcttgtcg aagcttgttt
    12721 ggaatgtgcc gctgacgggc aacggaaggg tgcgcggaac gccgcgtctg caagcctcgc
    12781 gcggcgcccc agcgactgca ctgacccata gcaaggagca agccattgac agcaagacgc
    12841 tgatttgaca tggcccgcga ccggggcgaa acagctgtcc gagcggcgtc acagccgccc
    12901 tgtcacggaa tcggtacgcc atccggcaca ggctgcgcgc cgtctggcgt atcgcggcct
    12961 cagcccggcc ggccgctgcg cttcagcagc ccccgctgcg ggtcgtagcc cagcgtagcc
    13021 gcgatgaaga acagcgccgc gctgccgagc acgactgtgc aggccagtgg ctcgaagcgc
    13081 ccgtacagcg cgtagcggat cagctcgacc gcatgcgtga acgggttcca ccgggcgatg
    13141 tgccagacga tctcggcgcc cgactcgcgc agcttccaca gcgggtacag cgccgaggaa
    13201 atgaagaaca tcgggaagat cacgaaattc atcgtgccgg cgaagttttc gagctgcttc
    13261 acgtacaccg acagcagcag cccgagcgca ccgagcatca gcccgccggc cgcgagcgcc
    13321 ggcagcaccg ccagccagcc gccggccgga atctcgacgc cgaaggccgc cgccaccagc
    13381 aggaaggcat aggcctgcag caccgacagc agcgtgccgg ccgcgagctt gcagaacagc
    13441 agccaccagc gcggcagcgg cgcggtcagc agcagccgca tcatgcccat ttcgcggtcg
    13501 tagaccatcg ccaggctcga ctgcatgccg ttgaacagca gcaccatgcc aagcaggccc
    13561 ggcgcgatgt agacgcggta ttcgatgtag gtgtcgtagg gcgggatgat cgcgacgccg
    13621 aacacgttct ggaaaccggc ggcgaacacg atcagccaca ggctcgggcg caccagcgac
    13681 gagaacagcc gcccgtactg gcggcagaac ttcgcgactt cgcggccggc aacggcgttg
    13741 agcgcgagca gggcgtgggc gggggtcatc ggcggacggg cctcggcagc gctgggaaag
    13801 gatgccgcgc agcttaaacg cctgcggaaa cgcccgcgaa ccccggcgcc tgcgccacga
    13861 cccgcaccgg tgcagccgcc ccgccggcgg gcgcaactgt caggagcctt gacgctggcc
    13921 ggccgacatg aacaagccgc acacgaccga tcagcgactt gggggccgtg tgcgccgatg
    13981 gcatcgggct tgtatcgctg catcgcagca tccgc
//
LOCUS       DAOQJH010000003        12180 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_2604_length_12180_cov_4.308536, whole genome shotgun sequence.
ACCESSION   DAOQJH010000003 DAOQJH010000000
VERSION     DAOQJH010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 12180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12180
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2604_length_12180_cov_4.308536"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..280)
                     /locus_tag="PLQ18_00180"
     CDS             complement(<1..280)
                     /locus_tag="PLQ18_00180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE00091.1"
                     /translation="MNSEQAILAAIERIPLPEPVERLIAYPEVDSMDRPAIRVWIILK
                     DDHVAERETSAMLDALTQAIRDRTWQIDERYWPYVRVRSVSEQALIESE"
     gene            complement(353..1057)
                     /locus_tag="PLQ18_00185"
     CDS             complement(353..1057)
                     /locus_tag="PLQ18_00185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE00092.1"
                     /translation="MTDRARTVRRPRWPLVLLPLLPLLLAATYNIRDRMSHGSWASFM
                     MYLGDDVLFRAAVTRGQDTFAVDSKLNTCDKFQLKVIGTLSKPSSDNTEFGAAVLIRV
                     DEAGLHQTSGQFSAERGSTTAFLLINRVEEGPRLFREMKYGNTVRFKVQPEGNNSPTF
                     MSFPLNGSAAAISRADDLCARYSNASGGGGGRGRNDGGGAPDDSDYFGGGGGGGQPQP
                     QPQPQRPKPRGDDSFF"
     gene            complement(1181..2626)
                     /gene="gatB"
                     /locus_tag="PLQ18_00190"
     CDS             complement(1181..2626)
                     /gene="gatB"
                     /locus_tag="PLQ18_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012981747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HNE00093.1"
                     /translation="MEWEAVIGLEVHTQLATKSKIFSGAATAYGAEPNRQACAIDLGL
                     PGVLPVLNAEAVRMAVMLGLAIDATVARRSVFARKNYFYPDLPKGYQISQYELPVVAR
                     GHLDILVDGVAKRIGITRAHLEEDAGKSLHEDFHGLSGIDLNRAGTPLLEIVSEPDLR
                     SAQEAVAYLKKLHQLVVWLGICDGNMQEGSFRCDANVSVRPKGQEQFGTRAEIKNVNS
                     FRFVERAIEYEIERQIELIEGGGRVVQETRLYDPDRDETRSMRGKEEANDYRYFPDPD
                     LLPLVIEDSFIEQLRASLPELPQAAFARFVRDYGLPDYDAATLTAERATAGYYEALVA
                     ATGGEVRLCAKLCANWVMGDLAAALNRAGLPIGESPVPAEALAGLLGRVLDGTLSGKL
                     AKEVFGAMWAGEGTADAIIAARGLVQLTDSSAIDTLIDGILAASPKQVEQYRAGQDKL
                     FGFFVGQVMKASKGKANPQQVNERLLARLKG"
     gene            complement(2815..4269)
                     /gene="gatA"
                     /locus_tag="PLQ18_00195"
     CDS             complement(2815..4269)
                     /gene="gatA"
                     /locus_tag="PLQ18_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018716501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HNE00094.1"
                     /translation="MHTQTLAELARGLAAGAFSSRELTEALLARIQAHDGALNSFITV
                     TPERALAQADAADAARARGEYGVLTGLPIAHKDIFCTQGVKTSCGSKMLDRFVAPYDA
                     TVVAKLDAAGMVMLGKTNMDEFAMGSSNESSWYGPVRNPWDVRAVPGGSSGGSAAAVA
                     ARLLPAATGTDTGGSIRQPAALCGITGLKPTYGRVSRWGMIAYASSLDQGGPLARTAE
                     DCALLLGAMAGFDARDSTSAGAPVPDYRAGLEAGLDGLRIGLPKEFFGDGLDAGVARA
                     VEAAIDEYRRLGADVVEVSLPNAQLAIPAYYVIAPCEASSNLARYDGVRFGHRCEAPR
                     DLVDLYTRSRAEGFGAEVQRRILVGTYALSAGYYDAYYLKAQQIRRLIHEDFRRAFEH
                     CDLIAGPTTPDVAFDLGEKADDPVALYLADIYTVAVNLAGLPALSHPVGDKGGRPVGL
                     QLIGPAFAEARLLNAAHRFQLATDWHRRAPQGFA"
     gene            complement(4427..4714)
                     /gene="gatC"
                     /locus_tag="PLQ18_00200"
     CDS             complement(4427..4714)
                     /gene="gatC"
                     /locus_tag="PLQ18_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004376355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HNE00095.1"
                     /translation="MSMGPEQVAATAQLARLALRAEDVPAVAGKLSAILDLVEQMNAV
                     DTAGIVPMAHPLELSQRLRADAVTESDRRADFQAIAPQTADGLYLVPRVLE"
     gene            4904..5953
                     /locus_tag="PLQ18_00205"
     CDS             4904..5953
                     /locus_tag="PLQ18_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005962807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HNE00096.1"
                     /translation="MFLRRLRGLFSNDLSIDLGTANTLIYARGQGIVLNEPSVVAIQQ
                     DATRGGPRTIAAVGTEAKKMLGRTPGNLSTIRPMKDGVIADFTVTEKMLQHFIRKVHA
                     RKFFNPSPRVLVCVPCGSTQVERKAIKESALGAGAREVFMIPEPMAAAIGAGMPVDEP
                     RGSMVVDIGGGTSEVAVISLNGIVYFESVRIGGDRFDEAITNYVRRNFGVLIGEPTAE
                     RIKQEIGSAYPTSDVREIEVKGRNLSEGVPRSFILNSNEILEALQEPLSGITGAVKKA
                     LESTPPELAADIAERGIVLTGGGALLRDIDRLLMEETGLNVVVAEDPLTCVARGGGRV
                     LELIDERGAQTFAVD"
     gene            6018..6977
                     /gene="mreC"
                     /locus_tag="PLQ18_00210"
     CDS             6018..6977
                     /gene="mreC"
                     /locus_tag="PLQ18_00210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HNE00097.1"
                     /translation="MGKLESRQSKSLFAPETAASIRYALLALLSVVLMTVDHRQHHLE
                     LMRNVLATALYPLQVTVQLPFEAADWLAQHFESRSALVEENERLREKQLFINAQLQKL
                     TALEAENRRLRSLLESTVNVAAERVLIAEILMVDFDPYRHQVLLNRGTQQGARIGQPL
                     IDERGIVGQVAHANLLDSTAILITDPAHALPVQLTRNGIRSLAVGTGRFSELELPNIP
                     NNGDVKVGDLVVTSGLGGRFPRGYPVARVTRVEFDPGRPFARVFAQPTAHLDRVREVL
                     MILDDIADPAAHPGAAAVAPVPDAAKPDRHPKPDKPAAAAGRH"
     gene            6982..7473
                     /gene="mreD"
                     /locus_tag="PLQ18_00215"
     CDS             6982..7473
                     /gene="mreD"
                     /locus_tag="PLQ18_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HNE00098.1"
                     /translation="MGSIETSRGYGLVLLTFVVALMLSSLHLPPVIDRFRPDWVGLVL
                     TYWALALPNRVGVGTGWVLGLIQDTAQGTLLGQHALGLATVAWLTVRFHQRIRVFPLW
                     QQALSVASFLMIEQLLVVWIKGITGYPPGDWWYLAPPLGGMLAWPWVFIVLRDLRRRF
                     QVS"
     gene            7494..9389
                     /gene="mrdA"
                     /locus_tag="PLQ18_00220"
     CDS             7494..9389
                     /gene="mrdA"
                     /locus_tag="PLQ18_00220"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012639261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HNE00099.1"
                     /translation="MSRPLRDTRAARKLFAGRALVAGVLVFLLFSVIAARLAWLQIVN
                     HEHFATLSDQNRIRLVPVPPTRGQIFDRKGVLLAGNLASFHLEITPEQVGSHAQMEAL
                     LEQLKTLIELSDSEINRFRRLLRRSPRYNGVPLRFNLTEDEMARVAINQFRLPGVDIR
                     NDLTRTYPLGTHMAHVVGYVGRIDEQELARLDPAEYSGSSHIGKIGVEKSYEDVLRGK
                     VGYQQVETNAQGRVIRVLESTPPTAGRNVYLTIDSRVQRVAELALSEANFNGAIVAID
                     PRNGELIAMASNPSYDPNPFVNGIDAKSYKALNTSPDRPLYNRALRGMYPPGSTIKPF
                     MGLAGLAYNAVDRYSSIFCGGYFQLPGQDHKFRDWRRGGHGRTDLDRAITESCDVYFY
                     DLALELGIDRIGAFLGQFGFGKPTGIDLRNEKSGLLPSQDWKRRTYNQAWFPGDTVSV
                     GIGQGYLLVTPLQLASATATLAMGGRRFQPRIVHALEDPALRRITELPAVALPSVAAP
                     DRRIFEWVTAFMTHVVHSERGTAFRAVGKFSAYRIAGKTGTAQVFTVGQNERYDASRL
                     KDELLDHALFMAFAPADAPRIAVAVIAENGGHGGSVAAPLAKKVMDAYLLPTNAAGAP
                     AGASPPP"
     gene            9397..10536
                     /gene="rodA"
                     /locus_tag="PLQ18_00225"
     CDS             9397..10536
                     /gene="rodA"
                     /locus_tag="PLQ18_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005962793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HNE00100.1"
                     /translation="MSPYAFQDAPLHDREAPRPRLLARVHLDAPLLLGLLAVAAIGLV
                     VLYSASLQDLESVIRQGTRLGLGFVVLIVLAQVPPTLLRTWTPALYAIGNLLLVAVLL
                     VGDHSMGAQRWLDLGVVRFQPSEIMKIAVPLMLAWHFADKQLPPRVPHLLVGAVIAGV
                     PFVLIAKQPDLGTGLVVFSAGLFALFLGGMSWKIIFGLIAAIAAVAPVFWEYGMREYQ
                     RQRVRTLFDPEADPLGAGYHIIQSKIAIGSGGLYGKGWLNGTQSHLEFLPESHTDFIL
                     GTFAEEFGLIGVCILLVLYFLIVARALRLAAGAGDTYGRIAGGTLALVFFFYAFVNTG
                     MVSGILPVVGVPLPLVSYGGTSLVTIMAGFGILMSLHFHRRRVGH"
     gene            10568..11617
                     /gene="mltB"
                     /locus_tag="PLQ18_00230"
     CDS             10568..11617
                     /gene="mltB"
                     /locus_tag="PLQ18_00230"
                     /inference="COORDINATES: protein motif:HMM:TIGR02282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HNE00101.1"
                     /translation="MIRLLSVAAAGLLLGACATATPPVVEARPRPVAAAEPATGQPFA
                     GRSEVDAFIREMTARHGIDAGALRRAFAQSASRPEIIELMDRPAEAKPWSAYRKIFLT
                     EKRIGGGVEFMRANAAALARAEARYGVPGQVITAIIGVETLYGANTGKFPVFEALATL
                     AFDYPRRADYFRKELENFLVLATEEGLDFRQPLGSYAGAMGLAQFMPSSYRRLAIDFD
                     GDGHRNLWTDPEDAIGSIANYLANSGWRRADPLIALPARLSGPGWEGAVSSNFRAPKS
                     LTRLSIAQWRARGVEPAAAVDAARTALLVTLDGETGLEPYLCFDNFYAITRYNLSALY
                     AMAVTQLSEAIAARR"
     gene            11638..>12180
                     /locus_tag="PLQ18_00235"
     CDS             11638..>12180
                     /locus_tag="PLQ18_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008931099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HNE00102.1"
                     /translation="MPANAKMRVTVRLLLGAGALWLAACSTPDSGDRRAEPPSRSGNP
                     ESYVVFGKRYQVLGTSEGYRERGIASWYGDAFHGKKTSSGVPFDMHEISAAHKTLPIP
                     TWVKVTNLRNGKSLVLRVNDRGPFVGDRVIDLSFAAARALDVTGRGTAEVELEAVAPF
                     QTLADAHRRPKPGDAGTLLAS"
BASE COUNT         1806 a         4334 c         4155 g         1885 t
ORIGIN      
        1 gctcactttc gatgagcgcc tgttcgctga cgctgcgcac gcgcacgtaa ggccagtagc
       61 gctcgtcgat ctgccacgtc cggtcacgga tcgcctgcgt cagcgcatcc agcatggcgc
      121 tcgtctcgcg ctcggcaacg tgatcatcct tcaggatgat ccagacgcgg atagccggtc
      181 gatccatcga atcgacctcc gggtaagcga tgagccgctc gactggttcc ggcaacggaa
      241 tccgttcgat cgcagcgagg atggcctgtt cgctgttcat gaccgcctca acccgtcagg
      301 caccggcaca cgcagccagc gcaaatcctc acatgaaata cttctggagt gctcagaaga
      361 agctgtcgtc gccacgcggt ttcggacgct gcggctgcgg ctggggttgc ggctgaccgc
      421 cgccaccacc gccaccgaag tagtcggaat catccggcgc accgccgccg tcgttgcggc
      481 cacgaccacc accgccgccg ctggcattcg aatagcgggc acagaggtcg tcggcgcgcg
      541 agatcgccgc cgccgagccg ttcagcggga agctcatgaa ggtcggcgag ttgttgccct
      601 cgggctgcac cttgaagcgg accgtgttgc cgtacttcat ctcgcggaac agccgcggac
      661 cttcctcgac gcggttgatc agcaggaagg ccgtggtgct gccgcgctcg gcgctgaact
      721 ggcccgaggt ctggtggagg ccggcctcgt cgacgcggat cagcacggcg gcgccgaact
      781 cggtgttgtc cgacgagggc ttcgacagcg tgccgatgac cttgagctgg aacttgtcgc
      841 aggtgttgag cttggaatcg accgcgaagg tgtcctggcc ccgcgtgacc gcagcgcgga
      901 acagcacgtc gtcgccgaga tacatcatga acgaggccca gctgccgtga ctcatgcggt
      961 cgcggatgtt gtaggtcgca gccagcagca gcggcagcag cggcagcagc acgagcggcc
     1021 agcgtggccg gcggacggtg cgcgcacgat cggtcatgga gtccccccgg ccggtggtcc
     1081 ggaatcggtt ctgaaggcgg atgcccgcct gtattaggtt ggctgagcgg gtccgggcga
     1141 tgtcctgccc ggggcccgct gacagtcagt gtaccggtga tcagcccttc agccgcgcga
     1201 gcagccgctc gttgacctgc tgcgggttgg ccttgccctt cgaggccttc atgacctggc
     1261 cgacgaagaa gccgaacagc ttgtcctgac cggcacggta ctgctcgacc tgcttcgggc
     1321 tggccgcgag gatgccgtcg atcagcgtgt cgatcgccga gctgtcggtg agctgcacca
     1381 gcccgcgcgc cgcgatgatc gcatcggccg tgccctcgcc cgcccacatc gcgccgaaca
     1441 cttccttggc gagcttgccg gagagcgtgc cgtcgagcac gcgcccgagc agcccggcga
     1501 gcgcttcggc cggcaccggg ctttcgccga tcgggaggcc ggcgcggttc agcgccgcgg
     1561 ccagatcgcc catgacccag ttggcacaga gctttgcgca gagcctgacc tcgccgccgg
     1621 tcgcggcgac cagcgcctcg tagtagccgg cggtggcgcg ctcggcggtc agcgtcgcgg
     1681 catcgtagtc aggcaggccg tagtcgcgca cgaagcgcgc gaaggccgcc tgcggcagtt
     1741 cgggcaggct cgcccgcagc tgttcgatga agctgtcctc gatcacgagc ggcagcaggt
     1801 cggggtccgg gaagtagcgg tagtcgttgg cttcttcctt gccgcgcatc gaacgggttt
     1861 cgtcgcgatc cgggtcgtag agccgggttt cctgcacgac gcggccgccg ccctcgatca
     1921 gctcgatctg gcgttcgatc tcgtattcga tcgcacgctc gacgaaacgg aagctgttga
     1981 cgttcttgat ctcggcacgc gtgccgaatt gctcctgccc cttcgggcgc accgagacgt
     2041 tggcgtcgca gcggaacgag ccttcctgca tgttgccgtc gcagatgccg agccagacca
     2101 cgagctggtg cagcttcttc aggtaggcga cggcttcctg cgccgagcgc aggtcgggct
     2161 cggagacgat ctccagcaac ggcgtgccgg cgcggttcag gtcgatgccg gagaggccgt
     2221 ggaagtcctc gtgcagcgac ttgccggcgt cctcctccag atgcgcgcgg gtgatgccga
     2281 tgcgcttcgc cacaccgtcg accaggatgt cgaggtggcc gcgggcgacc accggcagct
     2341 cgtactgcga gatctggtag cccttcggca ggtccggata gaagtaattc ttgcgcgcga
     2401 acacgctgcg ccgcgcgacc gtcgcgtcga tcgccagccc gagcatcacc gccatgcgca
     2461 cggcctcggc gttgagcacc ggcagcacgc cgggcaggcc gaggtcgatc gcgcaggcct
     2521 ggcggttcgg ttcggcgccg taggcggtgg ccgcgcccga gaagatcttc gatttcgtcg
     2581 cgagctgggt gtgcacctcc agcccgatga ctgcttccca ttccatccgt gcgtacctgg
     2641 agtgagtttg aaggagtgag ggatcaggcc cgggacctgc gcggggatga ctggcaggcg
     2701 gcgctcagca ggacagggat cggcgcgaag ccgcggcacc ggcagacgcc ggtgcggccg
     2761 cccgattccc caccgccctc atcgccgatc acgcttcatc ccctgacgct cccctcacgc
     2821 gaagccctgc ggcgcgcgcc ggtgccagtc ggtggcgagc tggaagcggt gcgcggcgtt
     2881 cagcaggcgc gcttcggcga aggccggacc gatcagctgc aggccgaccg gccgcccgcc
     2941 cttgtcaccg accgggtgcg acagcgccgg caggccggcg aggttgaccg cgaccgtgta
     3001 gatgtcggcc agatacaggg ccaccggatc atcggccttc tcgccgagat cgaaggcgac
     3061 atccggcgtg gtcggaccgg cgatcaggtc gcagtgctcg aaggcgcggc ggaagtcctc
     3121 gtggatcagc cggcggatct gctgcgcctt caggtaatag gcatcgtagt aaccggccga
     3181 cagcgcatac gtgccgacca ggatgcgccg ctgcacctcg gcaccgaaac cctcggcgcg
     3241 cgagcgcgtg tagagatcga ccaggtcgcg cggtgcttcg cagcggtgac cgaagcgcac
     3301 gccgtcgtag cgcgcgaggt tgctcgatgc ctcgcagggc gcgatcacgt agtaggccgg
     3361 gatcgccagc tgcgcgttcg gcaggctgac ctcgaccacg tcggcgccga gccggcggta
     3421 ttcgtcgatc gccgcctcga cggcgcgcgc gacgccggca tcgaggccgt caccgaagaa
     3481 ttccttcggc aggccgatgc gcaggccgtc gagcccggct tcgaggccgg cgcgatagtc
     3541 cggcaccggc gccccggcgc tggtcgagtc acgcgcatcg aaaccggcca tcgcgccgag
     3601 cagcagcgcg cagtcctcgg cggtgcgcgc gagcggtccg ccctgatcga ggctggaggc
     3661 gtaggcgatc atgccccagc gcgacacgcg cccgtacgtg ggcttgaggc cggtgatgcc
     3721 gcagagcgcg gccggctggc ggatcgagcc accggtgtcg gtgccggtcg ccgccggcag
     3781 caggcgcgcg gcgaccgctg ccgccgagcc gccggacgaa ccgcccggca ccgcccgtac
     3841 gtcccaggga ttgcgcaccg gaccgtacca gctcgactcg ttcgacgagc ccatcgcgaa
     3901 ctcgtccatg ttggtcttgc cgagcatgac catgccggcg gcatcgagct tcgcgaccac
     3961 ggtggcgtcg tagggcgcga cgaagcgatc gagcatcttc gagccgcagg aggtcttcac
     4021 gccctgcgtg cagaagatgt ccttgtgcgc gatcggcagg ccggtcagca cgccatattc
     4081 gccccgcgca cgcgcggcat cggcggcatc ggcctgcgcg agcgcgcgct cgggcgtgac
     4141 ggtgatgaag ctgttcagcg cgccgtcatg cgcctggatg cgcgcgagca gcgcctcggt
     4201 cagctcacgc gacgagaacg cgccggcggc gaggccgcgg gccagttcgg cgagggtctg
     4261 ggtgtgcatg ggcattcact tcccggtcgg ggacggggga cggcgcagga gcggatgagg
     4321 cgtcaggcgc gcggggggag acgcgggcag cgcaggacct ggacagggcc gaggcaggaa
     4381 atccgcagcg tctccctgcc tcttcctgcg acgcccgctg ccggcctcac tcgaggacgc
     4441 gcggcaccag atacaggccg tcggcggtct gcggcgcgat cgcctggaag tcggcgcgcc
     4501 ggtcggactc ggtgacggcg tcggcgcgca ggcgctggct cagttcgagc ggatgcgcca
     4561 tcggcacgat gccggcggtg tcgacggcat tcatctgttc gaccagatcg aggatcgccg
     4621 acagcttgcc ggccacggcc ggcacatcct cggcgcgcag ggccagacgc gcgagctgcg
     4681 cggtggcggc gacctgttcg gggcccatcg acatcggttc ctgactcctc tcgacagcgg
     4741 ttcggcagcc atgcgcccgt catggtgcag cgcgatgaaa tggccggcgc acggtagcac
     4801 ggatgccggc gcccgcccaa accccggccc cggactcgcc ttgcccggct accaagccgt
     4861 tgataccatg ccgcgccttt tccacggggc tacggcaagc acaatgttcc tcagacgtct
     4921 gcgcggactc ttctccaatg acctgtccat cgacctgggg accgccaata ccctgatcta
     4981 tgcccgcggg cagggcatcg tgctcaacga gccgtccgtg gtcgcgatcc agcaggacgc
     5041 cacgcgcggc gggccgcgca cgatcgccgc ggtcggcacc gaggccaaga agatgctcgg
     5101 gcgcacgccc ggcaacctca gcacgatccg gccgatgaag gacggcgtca tcgccgactt
     5161 caccgtcacc gagaagatgc tgcagcactt catccgcaag gtgcatgcgc gcaagttctt
     5221 caacccgagc ccgcgcgtgc tcgtttgcgt gccctgcggc tcgacgcagg tcgaacgcaa
     5281 ggcgatcaag gaatccgccc tcggcgccgg cgcgcgcgag gtgttcatga tccccgagcc
     5341 gatggccgcg gcgatcggcg ccggcatgcc ggtcgacgag ccgcgcggct cgatggtcgt
     5401 cgacatcggc ggcggcacct ccgaggtcgc ggtgatctcg ctcaacggca tcgtctactt
     5461 cgagtccgtg cgcatcggcg gcgaccgctt cgacgaggcg atcaccaact acgtgcgccg
     5521 caacttcggc gtgctgatcg gcgaaccgac cgccgagcgc atcaagcagg agatcggcag
     5581 cgcctacccg acctcggacg tgcgcgagat cgaggtcaag ggccgcaacc tctccgaggg
     5641 cgtgccgcgc agcttcatcc tgaactccaa cgagatcctc gaagccctgc aggagccgct
     5701 gtccggcatc accggcgcgg tcaagaaggc gctggaatcg acgccgccgg agctggccgc
     5761 cgacatcgcc gagcgcggca tcgtgctgac cggcggcggg gcgctgctgc gcgacatcga
     5821 ccggctgctg atggaggaga ccggcctcaa cgtcgtcgtc gccgaggacc cgctgacctg
     5881 cgtcgcccgt ggcggcggcc gggtgctcga actgatcgac gagcgcggcg cgcagacttt
     5941 cgcggtcgac tgaccgcctc ccctgcggcc gtctgcatcc gcggccgcgg gcctgctccc
     6001 gtcgccgggg ggacgccgtg ggcaagctgg aatcccgcca gtcgaaatcg ctgttcgcac
     6061 cggaaacggc cgcgtcgatc cgctatgcgc tgctcgcgct gctgtcggtg gtgctgatga
     6121 cggtcgacca ccgccagcac cacctggagc tgatgcgcaa cgtgctggcc accgcgctgt
     6181 acccgctgca ggtcaccgtg cagctgccgt tcgaggccgc cgactggctg gcgcagcact
     6241 tcgaatcgcg ctcggcgctg gtcgaggaga acgagcgcct gcgcgagaag cagctgttca
     6301 tcaatgccca gctgcagaag ctcaccgcgc tcgaggccga gaaccgccgg ctgcgctcgc
     6361 tgctggaatc gacggtcaac gtcgcggccg agcgcgtgct gatcgccgag atcctgatgg
     6421 tcgacttcga cccctaccgc caccaggtgc tgctcaaccg cggcacgcag cagggcgcgc
     6481 gcatcggcca gccgctgatc gacgagcgcg gcatcgtcgg ccaggtcgcg cacgccaacc
     6541 tgctcgattc gacggcgatc ctgatcaccg accccgcgca cgcgctgccg gtgcagctga
     6601 cgcgcaacgg catccgctcg ctcgcggtcg gcaccgggcg cttcagcgag ctggagctgc
     6661 cgaacatccc gaacaacggc gacgtcaagg tcggcgatct ggtcgtgacc tccggcctcg
     6721 gcgggcgctt tccgcgcggc tatccggtcg cgcgcgtcac gcgcgtcgag ttcgaccccg
     6781 gccggccgtt cgcgcgcgtg ttcgcgcagc cgaccgcgca tctcgatcgc gtgcgcgagg
     6841 tgctgatgat cctcgacgac atcgccgatc cggccgccca ccccggagcc gccgccgtcg
     6901 cgcccgtgcc ggatgccgcg aagccggatc gccatccgaa gcccgacaag ccggccgcgg
     6961 ccgcaggcag gcactgaaac catggggtcg atcgaaacct cgcgcggcta cgggctggtg
     7021 ctgctgacct tcgtcgtcgc gctgatgctc tccagcctgc acctgccgcc ggtcatcgac
     7081 cgcttccggc ccgactgggt cgggctggtg ctgacctact gggcgctggc gctgccgaac
     7141 cgcgtcggcg tcggcaccgg ctgggtgctc gggctgatcc aggacaccgc ccagggcacg
     7201 ctgctcggcc agcacgcgct cgggctggcg acggtcgcct ggctgaccgt gcgcttccac
     7261 cagcgcatcc gcgtgttccc gctgtggcag caggcgctgt cggtggcctc gttcctgatg
     7321 atcgaacagc tgctggtggt ctggatcaag ggcatcaccg gctatccgcc cggcgactgg
     7381 tggtatctgg ccccgccgct cggcggcatg ctggcctggc cgtgggtgtt catcgtgctg
     7441 cgcgacctgc gccggcgctt ccaggtttcc tgagcgtttc cggatcgagt gccatgagtc
     7501 gcccgcttcg cgacacgcgc gccgcgcgca agctgttcgc cggccgcgcc ctggtggccg
     7561 gcgtgctggt gttcctgctg ttctcggtga tcgccgcgcg tctggcctgg ctgcagatcg
     7621 tcaaccacga gcacttcgcg acgctgtcgg accagaaccg catccggctg gtgccggtgc
     7681 cgccgacgcg cggccagatc ttcgaccgca agggcgtgct gctcgccggc aacctggcgt
     7741 cgtttcatct ggagatcacg cccgagcagg tcggcagcca cgcgcagatg gaggcgctgc
     7801 tcgaacagct caagacgctg atcgagctgt cggactccga gatcaaccgc ttccggcgcc
     7861 tgctgcgccg cagcccgcgc tacaacggcg tgccgctgcg cttcaacctc accgaggatg
     7921 aaatggcgcg cgtggcgatc aaccagttcc ggctgcccgg cgtcgacatc aggaacgacc
     7981 tgacccgcac ctatccgctc gggacgcaca tggcgcacgt cgtcggctac gtcggacgca
     8041 tcgacgagca ggagcttgcg cgcctcgatc cggccgagta ctcggggtcg agccacatcg
     8101 gcaagatcgg cgtcgagaag tcctacgagg acgtgctgcg cggcaaggtc ggctaccagc
     8161 aggtggagac caacgcccag ggccgcgtga tccgcgtgct ggagagcacg ccgccgaccg
     8221 ccgggcgcaa cgtctacctg accatcgact cgcgcgtgca gcgcgtcgcc gaactcgcgc
     8281 tcagcgaggc gaacttcaac ggtgccatcg tcgcgatcga cccgcgcaac ggcgagctga
     8341 tcgcgatggc ctcgaacccg tcctacgatc cgaatccgtt cgtcaacggc atcgacgcca
     8401 agagctacaa ggcgctgaac acctcgccgg accggccgct gtacaaccgc gcgctgcgcg
     8461 gcatgtaccc gcccggctcg acgatcaagc cgttcatggg tctggccggc ctcgcctaca
     8521 acgccgtcga ccgctactcg tcgatcttct gcggcggcta cttccagctg ccggggcagg
     8581 atcacaagtt ccgcgactgg cgccgcggcg gccacggccg caccgacctc gaccgcgcga
     8641 tcaccgagtc ctgcgacgtg tacttctatg atctcgcgct cgaactcggc atcgaccgca
     8701 tcggggcctt cctcggccag ttcggcttcg gcaagccgac cggcatcgac ctgcgcaacg
     8761 agaagagcgg cctgctgccc tcgcaggact ggaagcggcg cacctacaac caggcctggt
     8821 tccccggcga cacggtcagc gtcggcatcg gccagggcta tctgctggtg acgccgctgc
     8881 agctcgcgag cgcgacggcg acgctggcga tgggcgggcg gcgcttccag ccgcgcatcg
     8941 tgcacgcgct cgaggacccg gcgctgcggc gcatcaccga gctgccggcg gtggcgctgc
     9001 caagcgtcgc cgcgccggac cggcgcatct tcgaatgggt cacggccttc atgacccacg
     9061 tcgtgcactc cgaacgcggc acggcgttcc gggcggtcgg caagttctcg gcctaccgca
     9121 tcgccggcaa gaccggcacc gcgcaggtgt tcacggtcgg ccagaacgag cgctacgacg
     9181 ccagccgcct gaaggatgaa ctgctcgatc acgcgctgtt catggccttc gcaccggccg
     9241 atgcgccgcg catcgccgtt gccgtgatcg ccgagaacgg cggccacggc ggctcggtgg
     9301 ccgcgccgct ggccaagaag gtcatggatg cctacctgct gccgaccaac gccgcgggcg
     9361 cccccgccgg cgcgagcccg ccgccatgag gtcgtcatga gtccgtacgc cttccaggat
     9421 gccccgctgc acgatcgcga ggcaccgcgc ccgcggctgc tggcgcgcgt gcacctcgat
     9481 gcgccgctgc tgctcggcct gctcgcggtc gccgcgatcg ggctggtcgt gctctacagc
     9541 gcctcgctgc aggatctgga gtcggtgatc cgccagggca cgcgcctcgg gctcggcttc
     9601 gtcgtgctga tcgtgctcgc gcaggtgccg ccgacgctgc tgcggacatg gacgccggcg
     9661 ctgtatgcga tcggcaacct gctgctggtt gcggtcctgc tggtcggcga tcactcgatg
     9721 ggcgcgcagc gctggctcga tctcggcgtc gtgcgcttcc agccctcgga aatcatgaag
     9781 atcgccgtgc cgctgatgct cgcgtggcac ttcgccgaca agcagttgcc gccgcgcgtg
     9841 ccacacctgc tggtcggtgc ggtgatcgcc ggcgtgccct tcgtgctgat cgccaagcag
     9901 ccggacctcg gcaccggcct cgtggtgttc tcggccgggc tgttcgcgct gtttctcggc
     9961 ggcatgtcgt ggaagatcat cttcggcctg atcgccgcga tcgctgcggt cgcgccggtg
    10021 ttctgggaat acggcatgcg cgaataccag cgccagcgcg tgcgcacgct gttcgacccg
    10081 gaggccgatc cgctcggtgc cggctaccac atcatccagt cgaagatcgc gatcggctcc
    10141 ggcggcctgt acggcaaggg ctggctgaac ggcacgcagt cacacctcga attcctgccc
    10201 gaatcgcaca ccgacttcat cctcggcacc ttcgccgagg agttcgggct gatcggcgtc
    10261 tgcatcctgc tggtgctgta cttcctgatc gtcgcgcgcg cgctgcgcct cgcggccggg
    10321 gccggcgaca cctacgggcg catcgccggc ggcacgctgg cgctggtgtt cttcttctat
    10381 gccttcgtca acaccggcat ggtcagcggc atcctgccgg tggtcggcgt gccgctgccg
    10441 ctggtcagct acggcggcac ctcgctcgtg acgatcatgg ccggcttcgg tatcctcatg
    10501 agcctccact tccaccgccg ccgcgtcggg cactgagcgc gacgcgccgg aagggcttcc
    10561 ctgccgcatg atccgcctcc tgtccgtggc cgccgccggc ctgctgctcg gcgcctgtgc
    10621 caccgccacg ccgcccgtcg tcgaagcccg cccgcgcccg gtcgccgctg ccgaaccggc
    10681 caccggccag cccttcgccg gtcgcagcga agtcgacgcc ttcatccgcg agatgaccgc
    10741 aagacacggc atcgacgccg gggcgctgcg ccgcgccttc gcgcagagcg cctcgcgccc
    10801 cgagatcatc gagctgatgg accgcccggc cgaagccaag ccgtggtcgg cctaccggaa
    10861 aatcttcctg accgaaaagc gcatcggcgg cggcgtcgag ttcatgcgcg ccaacgccgc
    10921 ggcgctggcg cgcgccgagg cccgctacgg cgtgcccggg caggtgatca ccgcgatcat
    10981 cggcgtcgag accctctacg gcgccaacac cggcaagttc ccggtgttcg aggcgctggc
    11041 gacgctggcc ttcgactacc cgcgccgcgc cgactacttc cgcaaggaac tggagaactt
    11101 cctggtgctc gcgaccgagg aaggtctcga cttccgccag ccgctcggct cctacgccgg
    11161 cgcgatgggg ctcgcgcagt tcatgccgtc gagctaccgg cgcctcgcga tcgacttcga
    11221 cggcgacggc caccgcaacc tctggaccga ccccgaggat gcgatcggca gcatcgccaa
    11281 ctacctcgcc aacagcggct ggcgccgcgc cgatccgctg atcgcgctgc cggcgcgcct
    11341 gagcggcccg ggctgggaag gtgcggtgtc gagcaacttc cgcgcgccga agtcgctgac
    11401 gcgcctgagc atcgcgcagt ggcgcgcgcg cggcgtcgag ccggccgcgg ccgtcgatgc
    11461 ggcgcgcacg gcgctgctgg tgacgctcga cggcgagacc ggactggagc cgtatctctg
    11521 cttcgacaac ttctacgcga tcacgcgcta caacctcagc gccctgtacg cgatggcagt
    11581 cacgcagctg tccgaagcga tcgcggcccg gcgctgagcc agcggagacg agggccgatg
    11641 cctgcgaacg cgaagatgcg agtcaccgtg cgactgctgc tgggggccgg cgcgctgtgg
    11701 ctggcggcat gctcgacgcc ggattcgggc gaccggcgcg ccgagccgcc gagccgctcc
    11761 ggcaaccccg agtcctacgt ggtgttcggc aagcgctatc aggtgctcgg caccagcgag
    11821 ggttaccgcg agcgcggcat cgcctcgtgg tatggcgatg ccttccacgg caagaagacc
    11881 tccagcggcg tgcccttcga catgcacgag atctcggccg cgcacaagac gctgccgatc
    11941 ccgacctggg tgaaggtcac gaacctgcgc aacggcaagt cgctggtgct gcgcgtcaac
    12001 gaccgcggcc ccttcgtcgg cgatcgcgtc atcgacctgt cgttcgccgc cgccagggcg
    12061 ctcgatgtca ccggccgcgg cacggccgag gtcgaactcg aagccgtcgc gcccttccag
    12121 accctcgccg atgcccaccg ccggccgaag cccggcgatg ccggcacgct gctcgcgtcg
//
LOCUS       DAOQJH010000004        10897 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_3097_length_10897_cov_5.475189, whole genome shotgun sequence.
ACCESSION   DAOQJH010000004 DAOQJH010000000
VERSION     DAOQJH010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 10897)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10897)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10897
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3097_length_10897_cov_5.475189"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(137..700)
                     /gene="ahpC"
                     /locus_tag="PLQ18_00240"
     CDS             complement(137..700)
                     /gene="ahpC"
                     /locus_tag="PLQ18_00240"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016780891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HNE00103.1"
                     /translation="MSLINTEVKPFKAQAYKDGKFVTVTEADLKGKWSVVCFYPADFT
                     FVCPTELEDLAAYYEEFQSRGVEIYSVSTDTHFAHKAWHDTSEAIGKVRYTMIGDPTG
                     AITRNFDVMIEDAGLALRGTFLINPDGVIKLCEIHDNGIGRDAGELLRKIKAAQYVHT
                     HPGEVCPAKWKEGEKTLTPSLDLVGKI"
     gene            949..1533
                     /locus_tag="PLQ18_00245"
     CDS             949..1533
                     /locus_tag="PLQ18_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018390589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HNE00104.1"
                     /translation="MMSRKVLMIVGDFAEDYETMVPFQTLLTLGYTVHAVCPERAAGE
                     RIRTAIHDFEGDQTYSEKPGHGFTLNASFADVSAADYDALLIPGGRAPEYLRMNARVL
                     ELVRAFDDAQKPIAAVCHGAQLLAAAGILKGRRVSAYPACAAEGKLAGAEDADIAIDA
                     AVTDGHLVTAPAWPAHPAWLAQFIARLGTTITHG"
     gene            1624..2154
                     /locus_tag="PLQ18_00250"
     CDS             1624..2154
                     /locus_tag="PLQ18_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015279592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HNE00105.1"
                     /translation="MTGAPAPFQKEHAMDWQEICASPLLRDLPFKIETDALGRIVMSP
                     ASNRHGLYQSRIAVLLSRLLPGGESQTECSVATADGVKVAGVVWRSDAFAARHGDATP
                     YPLAPEIVVEIVSPSNTVAELLRKKELYFARGAQEFWLCAGDGALRFFNNHSELAASA
                     LAPEFPATVARRPDAH"
     gene            complement(2191..2577)
                     /locus_tag="PLQ18_00255"
     CDS             complement(2191..2577)
                     /locus_tag="PLQ18_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007856334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE00106.1"
                     /translation="MSASILIVEDHGPSRQLLDFLLRARGYRTRLAGNGTEAMTTLER
                     EPIDLVLCDLHMPVMDGYVFIHRLRADARFEALPVLAVTASTLTGDRDRVLAAGFTGF
                     FSKPIDPEHFVEHMEAFLPPGQRAAG"
     gene            complement(2653..4152)
                     /locus_tag="PLQ18_00260"
     CDS             complement(2653..4152)
                     /locus_tag="PLQ18_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010459076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNE00107.1"
                     /translation="MSTVFQPSWADLAPDAVILSTEDGTVLQWAGGAETLYGYTHAAA
                     LGATIDELIVVPEDQAEQTQRLETVLAHGTLTFETIRRCRDGTLIYVDVSARCIATGN
                     DERGISLLWIEKDVTALRVRRDGRLLSARYHDLLESTPDGIVLADRTGHVVIANSHAE
                     HLFGYAPGELDGQPVEMLLPERLRSMHVTHRAHYRAQPHARPMGAGLELAARRRDGSE
                     FPVEISLSPLATEVGPMTMSAIRDVTERKRFEKALQEKNHELAAANQAKTQFLASMSH
                     ELRTPLNAIIGFTGALLMKLSGPLTSVQEEQLKLVQSSGKHLLALIGDLLDLARIEAG
                     RIDLTPEPLDAVAITNEVVASLRPQAVHKGLALNVAHEAPVLALHADRRALTQILINL
                     IGNAIKFTERGHVHVHSERAFDSDGRALVRIAIEDTGLGIRPEEQAQLFEAFARGLVA
                     KRAGIEGTGLGLHLSRKLTLLLGGNLSLRSEFGSGSTFTLELPAGPAAG"
     gene            complement(4245..4937)
                     /locus_tag="PLQ18_00265"
     CDS             complement(4245..4937)
                     /locus_tag="PLQ18_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNE00108.1"
                     /translation="MNEQPGTAVIAPGTSTAGVQDSVNGEGAACGGLICGTAKKAGRV
                     RDRIFATARELFSRHGVRGVGVDAIACEAGTNKMSFYRNFASKDDLVAECLSASESEF
                     WAWWDAQTQPHAGDPRRQIEALFDGFVTKVCTDQDRTRGCALANAVVEITEEDHPGRQ
                     VAVAFKAEVRRRLQLWAHELGAREPEMLGDALLLLMDGCYLSRLAFGDKGPVRSAGSA
                     ARALIAAQLAPA"
     gene            5130..6386
                     /locus_tag="PLQ18_00270"
     CDS             5130..6386
                     /locus_tag="PLQ18_00270"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNE00109.1"
                     /translation="MSRHTPNVRLARPLILSTPVAALVLALGLALSGCEDSVAVTPPP
                     APQAAPVTVAEVLSKPLRDWHEFTGRLEAESTVELRPRVSGYVDRVAFTEGARVKAGE
                     LLFAIDARPFRAEVARLAAERDRAAAELKLAQGNAARGQCLLPQRAIAQEEADRLTTL
                     AVTAQSALAASEAALTRARLDLEFTEVRAPVSGHVSRALITAGNLVTPGSVLTTVVAD
                     ERIHAYFDVDEQTFLRVGNDTGNARVQMGLASDSGYPHAGRIDFVDNRVDARTGTIRV
                     RALFDNADGRYTPGLYARVRLLGPELRDTVLIEDRAIGTDLDRRFVLVLGADGTVAYR
                     AVRLGPQIDGLRVVTDGLAAGDTIVVNGLQRVRPGAKVAATHVSMQLDDGGATLKQIA
                     GDDGAPDDRPAAHPAASRGPTRIAVR"
     gene            6531..9704
                     /locus_tag="PLQ18_00275"
     CDS             6531..9704
                     /locus_tag="PLQ18_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007954548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNE00110.1"
                     /translation="MNLSGFFISRPIFAGVLSALILIAGLISLPVLPISEYPDVVPPT
                     VVVRAQYPGANPVTIGETVASPLEQQINGVEGMLYQSSQATGDGTLTLTITFALGTDV
                     DKAQVLVQNRVAQALPKLPAEVQRLGVTTNKASPDLTMVVHLLSPDQRYDMLYLSNYA
                     ALRVKDELARLDGVGDVQLFGAGQFAMRVWLDPERLASRGLGAGDVLRAIREQNVQVV
                     AGSLGAPPAPNAADFQIAVDAGGRLASEEQFGNIVVKTGADGQLVRLRELGRVELGAD
                     SYALRSLLDNQPAVAMPIFQRPGSNAIAISDAVRAKMAELAKAFPQGVEYRIVYDPTV
                     FVRGSIEAVVHTLLEAVALVVIVVIVFLQTWRASIIPLLAVPVSLVGTFAVMHLFGFS
                     LNALSLFGLVLAIGIVVDDAIVVVENVERNIENGLKPIDATRQAMREVTGPIVATALV
                     LCAVFVPTAFISGLTGQFYRQFALTIAISTVISAFNSLTLSPALAARLLPEHGAAPDR
                     LTRLLNAALGWFFRPFNRGFARAAHGYTGLVRRTLRLSGIVLVLYAGLVALTYAGFTR
                     TPTGFVPGQDKQYLVAFAQLPDAATLDRTEDVIRRMSEIALAEPGALNAVAFPGLSIN
                     GFTNSPNSGIVFVTLKPFEERRSAELSAGAIAAKLNGKFAAIQDAFIAIFPPPPVMGL
                     GTIGGFRLQIEDRAGLGYAELFRQTQNLIAKSHERPELAGLLSSFQINVPQLKTDIDR
                     DKALAAGVAPADVFETLQVYLGSVYANDFNRFGRTYPVYVQAGAERRLEPADVLRLQT
                     RNRDGDMIPLGAFVSVTPGSGPDRVSHYNGYPTAEINGAAAPGFSSGQAQAAMEAIAR
                     AELPDGMSYEWTELTYQQLLAGDTTLYVYPLVVLLVFLVLAAQYESLRLPLAVILIVP
                     MCLLSALAGVNLAGSDNNVFTQIGLIVLVGLACKNAILIVEFAREREHEGEDVVQAVL
                     DACRLRLRPILMTSIAFIMGVVPLVLSSGAGAEMRHAMGVAVFAGMLGVTFFGLLLTP
                     VFYAVLRRGPARREQAAKSLPAPVAGH"
     gene            complement(9740..10126)
                     /locus_tag="PLQ18_00280"
     CDS             complement(9740..10126)
                     /locus_tag="PLQ18_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HNE00111.1"
                     /translation="MKLLIDMNLTPRWVAYLHDAGFAAEHWSMCGPVDASDASIMAFA
                     QTGDYVVLTHDLDFGAILAATHGQKPSVIQLRGEMPIPETIGSLLVQALEQLQSELVA
                     GALLTIDLKRARLRLLPLLLDNSGGR"
     gene            complement(10141..10368)
                     /locus_tag="PLQ18_00285"
     CDS             complement(10141..10368)
                     /locus_tag="PLQ18_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HNE00112.1"
                     /translation="MNEFERITQNPAVMGGKPCIRGMRVTVGAIVSQIGAGRCIEEVL
                     ADYPYLEREDVLQALRYAAWRLQEREIILGA"
     gene            complement(10452..>10897)
                     /locus_tag="PLQ18_00290"
     CDS             complement(10452..>10897)
                     /locus_tag="PLQ18_00290"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HNE00113.1"
                     /translation="VAALGWASGNLALRRAGAVNMLHFVVWMSLVPPLPLVLASLYFE
                     GPGALSRAWTAIDPIGAAALGYIVVASTWIGYGVWGWLMQRHAVTLVAPFSLLVPLNG
                     MLAAMLVYGEHYGPLRLVACALVLAGLTLNTFGGRLRWWRPALAS"
BASE COUNT         1614 a         3787 c         3777 g         1719 t
ORIGIN      
        1 atgggtggca tgggtggcgg cgtggtgtgc gtgcccgcgc agccggacga tcggtccgga
       61 agctgcgcgg gccccggtcg gggcggccgc cgccggggtg tccggcagcg cgccgctgaa
      121 gaggcagaca aaggcttcag atcttgccga cgaggtcgag cgacggcgtc agcgtcttct
      181 cgccttcctt ccacttggcc gggcagacct cgcccgggtg ggtgtgcacg tactgcgcgg
      241 ccttgatctt gcgcagcagc tcgccggcgt cacggccgat gccgttgtcg tggatctcgc
      301 agagcttgat cacgccgtcc gggttgatca ggaaggtgcc gcgcagcgcc agcccggcgt
      361 cctcgatcat cacgtcgaag ttgcgggtga tcgcgccggt cgggtcgccg atcatcgtgt
      421 agcggacctt gccgatcgcc tcggaggtgt cgtgccaggc cttgtgcgcg aagtgcgtgt
      481 cggtcgagac gctgtagatt tcgacgccgc gcgactggaa ctcctcgtag tacgcagcca
      541 gatcttccag ctcggtcggg cagacgaagg tgaagtcagc cgggtagaag cagaccaccg
      601 accacttgcc cttcagatcg gcctcggtga cggtgacgaa cttgccgtcc ttgtacgcct
      661 gggccttgaa cggcttcact tcggtattga tcagggacat cgtgcttgcc tcgctcaggg
      721 ggatgttcgg aagggacgac cgcagattac ggcgcgtatc gacgtagatg aaattgattg
      781 tggtcaatgg gtcgattgag tattgctatc gacaggatga cgaaatatcc accctgaatt
      841 catggaaatc accgcgcccg caggctacgc cgcagggggc gatccgcatc gccgcccagg
      901 catcgtttcc ccaccccggc cgctgcggcg gcccgtcccg aggagtacgt gatgagccgg
      961 aaagtgctga tgatcgtcgg cgatttcgcc gaggactacg aaaccatggt gccgttccag
     1021 acgctgctga cgctcggcta cacggtgcac gccgtctgcc cggagcgtgc cgccggcgag
     1081 cgcatcagga ccgcgattca tgatttcgag ggcgaccaga cctacagcga aaagcccggt
     1141 cacggcttca cgctgaacgc gagctttgcc gacgtgagcg ccgccgacta cgacgcgctg
     1201 ctgatcccgg gcggacgcgc gcccgagtac ctgcgcatga acgcgcgcgt gctggagctg
     1261 gttcgcgcct tcgacgatgc gcagaaaccg atcgctgctg tctgccacgg cgcgcagctg
     1321 ctggccgccg ccggcatcct gaagggccgg cgggtatcgg cgtatccagc ctgcgcggcc
     1381 gaggggaagc tcgccggcgc cgaggacgcc gacatcgcca tcgacgccgc cgtcaccgac
     1441 ggccatctgg tcaccgcccc ggcctggccg gcgcatccgg cctggctcgc gcagttcatc
     1501 gcgcggctcg gcacgacgat cacgcacggc tgataccggt ctttgctgaa cacgccgtct
     1561 tttcggcttc tccccctcgc gggggagggg ttgaggagag aggaatcaac cacgcaggca
     1621 cgcgtgaccg gtgcgccggc ccccttccag aaggagcacg cgatggactg gcaggaaatc
     1681 tgcgcctcac cgctgttgcg ggatctgccg ttcaagatcg agaccgacgc gctcgggagg
     1741 atcgtcatga gtccggcgag caaccgccac gggctgtatc agtcccggat cgccgtcctg
     1801 ctgagccggc tgctgccggg cggcgagagt cagaccgaat gcagcgtcgc gaccgccgat
     1861 ggcgtcaagg tggccggcgt ggtctggcgc agcgatgcct ttgcggcccg gcacggcgat
     1921 gccacgcctt atccgctggc ccccgagatc gtcgtcgaga tcgtctcgcc gtcgaacacg
     1981 gtcgccgaac tgctgcgcaa gaaggagctg tacttcgcac gcggcgcgca ggagttctgg
     2041 ctgtgtgccg gggacggcgc gctgcgcttc ttcaacaatc acagcgagct ggcggcgagt
     2101 gcgctggcgc cggagttccc ggccacggtc gcgcgccggc cggacgcgca ctgaagcacg
     2161 ggcttgccac gtggcaagtc cggctcgctt tcagccggcg gcgcgctgcc cgggcggcag
     2221 gaaggcttcc atgtgctcga cgaaatgctc cggatcgatc ggcttcgaga agaagccggt
     2281 gaagccggcg gccagcacgc ggtcgcggtc gccggtcagc gtcgaggcgg tgacggcgag
     2341 caccggcagg gcctcgaagc gcgcatcggc gcgcagccgg tggatgaaca cgtagccgtc
     2401 catgaccggc atgtgcaggt cgcagagcac gaggtcgatc ggctcgcgtt cgagcgtcgt
     2461 catcgcctcg gtgccgttgc cggcgaggcg cgtgcgatag ccgcgtgcgc gcagcaggaa
     2521 gtcgagcagc tggcggctcg gaccgtggtc ttcgacgatc aggatgctgg cactcatcgc
     2581 gcggaccggg gtggagaacg ggaagcagcc tacgttaccc gatcggcccg gtgatcgtgg
     2641 catccgccgg cctcagccgg cggcgggacc ggccggcagt tccagcgtga aggtgctgcc
     2701 gctgccgaat tcgctgcgca gcgacaggtt gccgccgagc agcagcgtca gcttgcggct
     2761 caggtgcagg ccgaggccgg tgccctcgat gccggcgcgc ttggcgacca ggccgcgcgc
     2821 gaaggcctcg aacagctgcg cctgttcctc ggggcggatg ccgaggccgg tgtcctcgat
     2881 cgcgatgcgc acgagcgccc ggccgtcgct gtcgaaggcg cgttcgctgt gcacgtgcac
     2941 gtggccgcgc tcggtgaact tgatggcgtt gccgatcagg ttgatcagga tctgcgtcag
     3001 ggcccggcga tcggcgtgca gcgccagcac cggcgcctcg tgcgcgacgt tcagcgccag
     3061 ccccttgtgc acggcctgcg ggcgcagcga ggcgacgacc tcgttggtga tggcgacggc
     3121 atcgagcggc tccggcgtca ggtcgatgcg cccggcctcg atgcgcgcca ggtcgagcag
     3181 gtcaccgatc agcgccagca ggtgcttgcc gctcgactgc acgagcttga gctgttcttc
     3241 ctgcacgctg gtcagcggac cggagagctt catcagcagc gcgccggtga agccgatgat
     3301 ggcgttcagc ggcgtgcgca gctcgtggct catcgaggcc agaaactggg tcttggcctg
     3361 gttggcggcg gcgagctcgt ggttcttctc ctgcagggcc ttttcgaagc gcttgcgctc
     3421 ggtgacgtcg cggatcgccg acatcgtcat cggcccgacc tcggtggcaa gcgggctcag
     3481 gctgatctcg accgggaatt cgctgccgtc gcgccggcgc gcggccagtt cgaggccggc
     3541 acccatcggt cgcgcgtgcg gctgcgcgcg gtaatgcgca cggtgcgtga cgtgcatcga
     3601 gcgcaggcgc tcgggcagca gcatctcgac cggctggccg tcgagctcgc cgggtgcgta
     3661 gccgaacagg tgttcggcgt ggctgttggc gatgaccacg tggccggtgc gatcggcgag
     3721 cacgatgccg tccggcgtcg attcgagcag gtcgtggtag cgggcgctga gcaggcggcc
     3781 gtcgcggcgc acgcgcagcg ccgtcacgtc cttctcgatc cacagcaggc tgatgccgcg
     3841 ctcgtcgttg ccggtggcga tgcagcgggc cgagacatcg acgtagatca gcgtgccgtc
     3901 gcggcagcgg cggatggtct cgaaggtcag cgtgccgtgg gcgagcaccg tttcgaggcg
     3961 ctgcgtctgc tcggcctgat cctcgggcac gacgatcagc tcgtcgatcg tggcgccgag
     4021 cgcggcggcg tgcgtgtagc cgtaaagcgt ctcggcgccg ccggcccact gcaggacggt
     4081 gccgtcctcg gtgctgagga tcacggcatc gggcgcgaga tcggcccagg aaggctggaa
     4141 aacggtgctc aagtcgctgg tctccgcagg ccgggcgggt ggatggccgc accatacgcg
     4201 gggaaacgct gaaaacaata cggccggccc gcgcggcacc ggcttcaggc gggtgcgagt
     4261 tgcgcggcga tcagcgcccg cgccgcgctg ccggcgctgc gcaccggccc cttgtcgccg
     4321 aaggcgagcc gcgacagata gcagccgtcc atcagcagca gcagcgcgtc gccgagcatc
     4381 tcgggctcgc gcgcaccgag ctcgtgcgcc cagagctgca ggcggcggcg cacttcggcc
     4441 ttgaaggcca cggccacctg acggccgggg tgatcctcct cggtgatctc gacgacggcg
     4501 ttggcgagcg cgcagccgcg cgtgcggtcc tgatcggtgc agaccttggt gacgaagcca
     4561 tcgaacagcg cctcgatctg gcggcgcggg tcgccggcgt gcggctgcgt ctgcgcgtcc
     4621 caccaggccc agaactcgct ttccgaggcg ctcaggcact cggcgaccag atcgtccttg
     4681 gatgcgaagt tccggtagaa gctcatcttg ttggtgccgg cctcgcaggc gatggcatcg
     4741 acgccgacgc cgcgcacgcc gtggcgcgag aacagctcgc gggcggtcgc gaagatgcgg
     4801 tcgcgcacgc gcccggcctt tttcgccgtg ccgcagatca ggccgccgca ggcggcgcct
     4861 tccccgttca cggaatcctg cactccggcg gtactcgtgc cgggtgcgat gacggcagtg
     4921 cctggctgct cgttcatgtt tgcgctcctg ttgacatgtg actgaccggt aactaacgta
     4981 gctgcacatg ttaccggtcg gtaacggctt gtacagcatc ggtcccgcgc gagttcccgt
     5041 caaggatggg ccggatcgca ccggatgccc gccggatacg cgctgaaccg ctgcccgttc
     5101 ctgatctccg cccccgggag tccgccgcca tgtcccgcca cacaccgaac gtcaggcttg
     5161 cccgtcccct gatcctgtcc accccggtcg ccgcgctggt gctggcgctc ggcctggcgc
     5221 tgtccggttg cgaggacagc gtcgccgtca cgccgccgcc ggctccgcag gccgcgccgg
     5281 tgacggtcgc cgaggtgctg agcaagccgc tgcgcgactg gcacgagttc accggccggc
     5341 tcgaagccga gagcacggtc gagctgcgcc cgcgcgtcag cggctacgtc gatcgcgtcg
     5401 ccttcaccga gggcgcgcgc gtcaaggccg gcgagctgct gttcgcgatc gacgcgcggc
     5461 cgttccgggc cgaggtcgcg cggcttgcgg ccgaacgcga tcgcgccgcg gcggagctga
     5521 agctcgcgca gggcaacgcc gcgcgcggcc agtgcctgct gccgcagcgc gcgatcgcgc
     5581 aggaagaagc cgaccgcctg acgacgctcg ccgtcaccgc gcagagcgcg ctcgccgcca
     5641 gcgaggcggc gctgacgcgc gccaggcttg atctcgaatt caccgaggtg cgcgcgccgg
     5701 tttccggcca cgtgtcgcgg gcgctgatca ccgccggcaa cctggtcacg cccgggagcg
     5761 tgctgaccac cgtcgtcgcc gatgagcgca tccacgccta cttcgatgtc gacgagcaga
     5821 ccttcctgcg cgtcggcaac gacaccggca acgcccgcgt gcagatgggg cttgccagcg
     5881 acagcggcta tccgcacgcc ggacgcatcg acttcgtcga caaccgcgtc gatgcccgca
     5941 ccggcacgat ccgcgtgcgc gcgctgttcg acaacgctga cggccgctac acgccgggcc
     6001 tgtatgcgcg cgtgcgcctg ctcgggcccg agctgcgcga caccgtgctg atcgaggatc
     6061 gcgccatcgg caccgacctc gaccgccgct tcgtgctggt gctcggcgcc gacggcacgg
     6121 tcgcctaccg cgccgtgcgc ctcggcccgc agatcgacgg cctgcgcgtg gtcaccgacg
     6181 gccttgcggc cggcgacacc atcgtcgtca acggcctgca gcgcgtgcgt ccgggcgcga
     6241 aggtcgcggc gacgcacgtg tcgatgcagc tcgatgacgg cggcgcgacg ctgaagcaga
     6301 tcgccggcga tgacggcgcg ccggacgaca ggccggccgc gcatccggcc gcgagccgcg
     6361 gaccgacgcg catcgcggtg cgctgaagcc gcaacggccg ccggcgcggc ggccgtgact
     6421 cccctgcggt tccccggatc cccgaacgcc ccagcccggc gctgcgcccg cgcgccgccg
     6481 gatctccccg cctgtgcccg cacgcaggtg caaccgaagc cggagccctc atgaacctgt
     6541 ccggattctt catctcccgc ccgatcttcg ccggcgtgct gtcggcgctg atcctgatcg
     6601 ccggcctgat ctcgctgccg gtgctgccga tttcggaata tccggacgtg gtgccgccga
     6661 ccgtggtcgt gcgcgcgcaa taccccggcg ccaacccggt cacgatcggc gagacggtcg
     6721 caagcccgct ggagcagcag atcaacggcg tcgagggcat gctgtaccag tcctcgcagg
     6781 cgaccggtga cggcacgctg acgctgacca tcacgtttgc gctcggcacc gatgtcgaca
     6841 aggcgcaggt gctggtgcag aaccgcgtcg cgcaggcgct gccgaagctg ccggccgagg
     6901 tgcagcgcct cggcgtgacc accaacaagg cctcgcccga cctgaccatg gtcgtgcacc
     6961 tgctctcgcc cgatcagcgc tacgacatgc tgtacctgtc gaactacgcg gcgctgcggg
     7021 tcaaggacga gctcgcccgc ctcgatggcg tcggtgacgt gcaactcttc ggcgccggcc
     7081 agttcgcgat gcgcgtctgg ctcgatccgg agcggctcgc gagccggggc ctcggcgccg
     7141 gcgacgtgct gcgcgcgatc cgcgagcaga acgtgcaggt ggtcgccggc agcctcggcg
     7201 cgccgccggc gccgaacgcc gccgacttcc agatcgccgt cgatgccggc gggcgcctcg
     7261 cgagcgagga gcagttcggc aacatcgtcg tcaagaccgg cgccgacggc cagctcgtgc
     7321 gcctgcgcga gctgggacgc gtcgagctcg gcgccgattc ctacgcgctg cgctcgctgc
     7381 tcgacaacca gccggcggtg gcgatgccga tcttccagcg ccccggcagc aacgccatcg
     7441 ccatctccga cgccgtgcgc gcgaagatgg ccgaactcgc gaaagccttc ccgcagggcg
     7501 tcgagtaccg catcgtctac gacccgaccg tgttcgtgcg cggctcgatc gaggccgtcg
     7561 tgcacacgct gctcgaagcc gtggcgctcg tcgtcatcgt cgtgatcgtg ttcctgcaga
     7621 cctggcgcgc gtcgatcatc ccgctgctgg cggtgccggt atcgctggtc ggcaccttcg
     7681 cggtcatgca cctgttcggc ttctcgctga acgcgctgag cctgttcgga ctggtgctgg
     7741 cgatcggcat cgtcgtcgat gacgcgatcg tcgtggtcga gaacgtcgag cgcaacatcg
     7801 agaacggcct caagcccatc gacgccacgc gccaggcgat gcgcgaggtc accggcccga
     7861 tcgtcgcgac cgcgctggtg ctctgcgcgg tgttcgtgcc gacggccttc atcagcggcc
     7921 tgaccgggca gttctaccgg cagttcgcgc tgaccatcgc catcagcacc gtgatctcgg
     7981 ccttcaactc gctgacgctg tcgccggcgc tcgccgcacg cctgctgccc gagcacggcg
     8041 cggcgcccga ccggctcacc aggctgctca acgccgcgct cggctggttc ttccggccgt
     8101 tcaaccgcgg cttcgcgcgc gccgcgcacg gctacaccgg cctcgtgcgc cgcacgctgc
     8161 ggctgtcggg catcgtgctg gtgctgtacg ccgggctggt cgcgctgacg tatgccggct
     8221 tcacgcgcac gccgaccggt ttcgtgccgg gccaggacaa gcagtatctg gtcgccttcg
     8281 cgcagctgcc ggacgccgcc accctcgatc gcaccgaaga cgtgatccgg cgcatgagcg
     8341 agatcgcgct tgccgagccg ggcgcgctga acgccgtggc tttcccgggc ctgtcgatca
     8401 acggcttcac caacagcccg aacagcggca tcgtgttcgt gacgctgaaa cccttcgagg
     8461 aacgccgctc ggcggagctg tcggccgggg cgatcgccgc caagctcaac ggcaagttcg
     8521 ccgccatcca ggacgccttc atcgccatct tcccgccgcc gccggtcatg ggcctcggca
     8581 cgatcggcgg cttccgtctg cagatcgagg atcgcgccgg cctcggctat gccgagctgt
     8641 tccgccagac gcagaacctc attgcaaaga gccacgaacg cccggaactc gccggcctgc
     8701 tgtcgagctt ccagatcaac gtgccgcagt tgaagaccga catcgaccgc gacaaggcgc
     8761 tggccgccgg ggtcgcgccg gcggacgtgt tcgagacgct gcaggtgtat ctcggctcgg
     8821 tctacgccaa cgacttcaac cgcttcgggc gcacctaccc ggtctacgtg caggccggcg
     8881 ccgaacgccg gctggagccc gccgacgtgc tgcgcctgca gacgcgcaac cgcgacggcg
     8941 acatgatccc gctcggtgcc ttcgtcagcg tcacgcccgg ctcggggccg gatcgcgtca
     9001 gccactacaa cggctacccg accgccgaga tcaacggcgc cgcggcaccg ggcttcagca
     9061 gcggccaggc gcaggcggcg atggaggcga tcgcgcgtgc cgagctgccg gacggcatga
     9121 gctacgaatg gaccgagctg acctatcagc agctgctcgc cggcgacacg acgctgtacg
     9181 tgtatccgct ggtcgtactg ctggtgttcc tggtgctcgc cgcgcagtac gagagcctgc
     9241 gcctgccgct ggcggtgatc ctgatcgtgc cgatgtgcct gctgtcggcg ctcgccggcg
     9301 tcaatctcgc cggcagcgac aacaacgtgt tcacgcagat cggcctgatc gtgctggtcg
     9361 gcctcgcctg caagaacgcg atcctgatcg tcgagttcgc gcgcgaacgc gagcacgagg
     9421 gcgaggacgt ggtccaggcc gtgctcgacg cctgccgcct gcgcctgcgc ccgatcctga
     9481 tgacctcgat cgcgttcatc atgggcgtgg tgccgctggt gctctcgagc ggcgccgggg
     9541 ccgagatgcg tcacgcgatg ggcgtggcgg tgttcgccgg catgctcggc gtgaccttct
     9601 tcgggctgct gctgacgccg gtgttctacg cggtgctgcg ccgcgggccg gcgcggcggg
     9661 agcaggcggc gaagtcgctg ccggcgccgg tggcggggca ctgagccgga gccttccggc
     9721 gggcgacggg ccgttccggt cagcgcccgc cggaattatc cagcagcagt ggcagtaaac
     9781 gcagtcgtgc ccgtttcaga tcgatggtca gcaaggcacc ggcaaccagt tcggactgca
     9841 gttgttccag tgcctgaacg agcaggctgc cgatggtttc cggaatcggc atctcgcctc
     9901 tgagctggat gacgcttggc ttttggccgt gcgttgcggc gaggatggcg ccgaagtcga
     9961 gatcgtgtgt cagcacgacg taatcacccg tctgtgcaaa agccatgatg cttgcatcgg
    10021 acgcatcgac cggaccacac attgaccagt gctcggctgc aaaccctgcg tcgtgcaggt
    10081 aagccaccca gcgcggcgtc aggttcatgt cgatcagcag tttcatgcct ggtcgtcacg
    10141 ctaggcgccg agaatgattt cacgttcctg cagacgccag gcggcataac gcagcgcctg
    10201 caggacatcc tcgcgttcca gatagggata gtcggccagc acctcctcga tgcaccggcc
    10261 cgcaccgatc tggctgacga ttgcacccac ggtcacgcgc atgccacgaa tgcacggttt
    10321 gccgcccatg accgccgggt tctgggtgat gcgttcgaac tcgttcatgg cgtcgatcct
    10381 ccgggtctct gcggcgttgc gcacgatgga agtatcgcag gccggtcagg atcagcgcat
    10441 gcagtatccg gtcagcttgc cagcgccggc cgccaccacc gcagccgccc gccgaaggtg
    10501 ttcagcgtca gccccgccag caccagcgcg caggcgacga ggcgcagcgg cccgtagtgc
    10561 tcgccgtaga ccagcatcgc cgccagcatg ccgttcagcg gcaccagcag cgagaacggc
    10621 gcgaccagcg tcaccgcgtg gcgctgcatc agccagcccc agacgccgta accgatccag
    10681 gtcgaggcga cgacgatgta gccgagcgcg gcggccccga tcgggtcgat cgccgtccac
    10741 gcccgcgaca gcgccccggg gccttcgaag tacagcgagg ccagcaccag cggcagcggc
    10801 ggcaccaggc tcatccagac cacgaagtgc agcatgttga ccgccccggc ccggcgcagc
    10861 gccagattgc cgctcgccca gccaagcgcg gcaacca
//
LOCUS       DAOQJH010000005        10151 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_3456_length_10151_cov_3.684231, whole genome shotgun sequence.
ACCESSION   DAOQJH010000005 DAOQJH010000000
VERSION     DAOQJH010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 10151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10151
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3456_length_10151_cov_3.684231"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            311..2257
                     /locus_tag="PLQ18_00295"
     CDS             311..2257
                     /locus_tag="PLQ18_00295"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNE00114.1"
                     /translation="MTATLPQVPSTALESVPGSASLSSRWRNLPLRHQFHALLTIVVL
                     ALLLLVAGTLLGLRRDLDSQAREALAASLQSVMTVLEHFADAEGSARMDRAQAQARAI
                     EALTALRQGDTYVFAFDRDGRFVLHGGDPAQVGQPVRGLRDAEGRDLYEAFAGAAHRD
                     ASGSGRGDFVEYPYVRPSGTKPERKLSHVRTFEPWGWVLGTGVYLTDLDSRFARQRNT
                     LVAGVAIVAVLLGSLLTWQSRRIVGRVQAVLGFAQRLAVHDLQAHMRVDTQEEFGRMA
                     AALNAAVDSLREAAARDQAQMEQLRAAEAAREVQSREIAAQADCIREAAERDRAAADE
                     LRTKVGRIVVAVEAAASGELTVTPGVGGNDAIGRVGEAFGHLLHDLRSSIAGIGRNAE
                     ALGRSVEVARNTGQRLNGVAGDTSARAERLSGNASAVDDSLQSVASATEEMNAAVQEI
                     SRHVHRAVGIAAVAAQAADHATGLVGALTTSSAEIGSVSRTITGIAEQTKLLALNATI
                     EAARAGEAGKGFAVVANEVKELARGTEQATADIEKRIGTIQHDTGHVVHAITDIRRTI
                     DEVNEISAMIAAAIEEQSATTREISRSVALAAQGSAEITAFVRQLADTAQAVHTEAGA
                     TTQAADQIARIAEDQIQLIRRFRI"
     gene            2447..7213
                     /locus_tag="PLQ18_00300"
     CDS             2447..7213
                     /locus_tag="PLQ18_00300"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNE00115.1"
                     /translation="MTFSPRRLFWPLVLLASAVGLCGLLGIQYTNVRQLDSDAHAFAR
                     QIELLRNAFGYGGFIHHFKNFLLRRTQASYALAGAGLDSLGVTAAELEQFTHDLAERE
                     ALHEIGRVIGRYRTAWQLAGLPTHREMQPEALDGLVRVEDTVAISALGILEAAAQQRL
                     AQARAASTQLQMLLAASLVALPLLALVYLHQQRALRRESRLADCLAHSERELRQSNEQ
                     MRAIFDGMVHLICLLRPDGTLIELNRSAALFAGQARDTLLGLQLASLPWWPRSPDAQA
                     AVQRALAVAGSGQCFREEFPVLGPGGFRATLDVSLQPLRDEHGEIVRLVLEARDLSER
                     QRLEAGIRAEGERARRYLDIARSIILARSIDGRIQLINREGCELIGLPAGQLIGRNWF
                     ETCVPQGERTRAQVVFAQVITGFDPLIEEERALLTASGETRCIAWRHTVVCNETGVVV
                     AILSAGRDVSTERAAELHLRSINVELERRVRERTRALEDARDALLGRQHRLGILKTLA
                     EQAGSAADLSVVLNLTLRLVCEHLAWPVGHAWIRDAVDQPLRSSHCWHLGDPEIYAGF
                     QARSQALEFGPGVGVIGRVLEAGEAQWIEDLATDVHFLRHDPLVRSGLCGGGFCPVRV
                     RGEVRLLLEFFSTDDNGVTGEIRDFVHQVATLLGMIAETLLDRAELRRLALFASETDD
                     MLVVTDREGRIEWVNQGFSDCTGWTCAEALGQHPGHLIYGPRTDAGTIAGMQAAMRAG
                     DKIGTELVVYRRSGEPFWVALTVQPVYDAAGRLERYLSVAHDISQRHQADELLRSREQ
                     ELRSLFENVIDGIITMDAKGRVRAFNPAAERLFGYEAAEVIGHNVSMLMPEPHRSAHD
                     GYLARYVAGGRAAIIGIGREVEGLHRDGRRLEIDLAVNECELNGERCFIGIVRDIAER
                     RRITAELERARDAAEQANRAKSTFLATMSHEIRTPMNGVIGMLEVLGHETLTPAQTDT
                     VATIRQSAHTLLSIIDDVLDFSKIEAGHLALEHAPLPLAAIIESIGDTLAPIAASKQV
                     TLEIYVDPRLPEWILGDAVRLRQVLYNLAGNAVKFTGEQTDRPGCVALRAECGTAGGL
                     RLRVIDTGISMSAQTIERLFQPFMQAEASTTRRFGGTGLGLSIVARLIDLMGGRIGVD
                     STPGLGSTFTIELPLEAAGGQPSIAPRYAFAGLPVSLLCGGDRLGADLGEYLAAAGAR
                     VLPAADVDTLVQDSAAAGDARLAIVVVDGRCHGADSIERLRGQFAAQAGRRDVRYVLI
                     THGRRRESRIEGDDLITLDGNVLHRHVFLRAVAIAAGLASPVTPAVETMLPLPAAAPP
                     GIEAARTAGRLILVAEDNAINRKVLERQLALLGHAAEFVADGRAALSRWRSHRHALLI
                     TDLHMPELDGYELAATIRHEESASGSRRRPIIAFTANALKGEVEHCRSVGMDDCLIKP
                     LQIERLRTALERWLPVASVPAAAAPVPEAPPPRSGERPPPFDPLALQELIGDDAELIT
                     GLLDEFLDGVRANLDALRQLQADQDLTGIADIGHRLKSSARAVGAAELGMLYEQLEQA
                     GRADDGPAIDAVLAALAPAIARLTARIEPHAWNPCT"
     gene            7210..8460
                     /locus_tag="PLQ18_00305"
     CDS             7210..8460
                     /locus_tag="PLQ18_00305"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018991537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing response regulator"
                     /protein_id="HNE00116.1"
                     /translation="MNTDCLPDGDDRKQDRKNDAEILLLDDEPLLLRVLALALNACGY
                     RSITSCANAADALLRLDDAALPPIELIFCDLNMPGMDGVQFVRALGERGWQGSLVLIS
                     GEERRTVDTVARLASAHRLDLLGQMPKPVVRAQLVELLERWRTRQPAPGPGAQAGEVE
                     PGELRRALAAGEFVNVYQPKMAFAGGGIEGVEALVRWQHPVAGIIYPDRFIGVAERHG
                     LIDALTQAVCARALADASDWQRQGLGLSVAINVSMDSLRDLDFPERIAAEVRRAAFNP
                     AQLVLELTESRLPPNPVVLLDTLTRLRLKRYRLSIDDFGTGYSTLAQLRDIPFDELKI
                     DRGFVHRAHADPARGAILAASLGMAHQLGLRTVAEGIEDAADWQHVQRLGCDLGQGYH
                     IARPMPPAALPAWVAARSLSGQAR"
     gene            8681..>10151
                     /locus_tag="PLQ18_00310"
     CDS             8681..>10151
                     /locus_tag="PLQ18_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013955377.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HNE00117.1"
                     /translation="MNAVAGIQGRERYRPVHKLRFVTAAALFDGHDASINLMRRILQA
                     QGTEVIHLGHNRSVESVVSAALDEDVQGIAVSSYQGGHVEYFRYMIDLLRARGGGHIR
                     VYGGGGGVIVPAEIAELQDYGVTRLFSPEDGQRLGLAGMINLMVEACDVDLAAQAPAT
                     LDGLRAGDRLALARWLSVLEAGRMAASLRDELLQAAQARRAPVLGITGTGGAGKSSLT
                     DELIRRFRLDRDEALKIAVLAADPVRRRSGGALLGDRIRMNAIEHPNLYLRSLATPAG
                     GSELPQRLDELVAACRVAGFDLVIIETPGIGQGAAGIVAHADLALYVMTPEYGAASQL
                     EKIDMLDFADVVAINKYDRPGGADALRDVRRQVQRNREAWGTPPADLPVFGTIASRFN
                     DDGVTALYQHLSERLAALGLPPAGGRLAAVTTRASTGSRALLPPARVRHLAAIAETVR
                     AYHRRVDEQAGLVRSLQQVEAVQAMLRASEARHRHLFEAA"
BASE COUNT         1576 a         3488 c         3522 g         1565 t
ORIGIN      
        1 tttgtttcac gcatgtttca cgcacggggg agctttctcc cggcatggcg cctgtcgcgg
       61 caggtttcat cgggagtttt ttaaaagcaa cggaatacgt tcaagcctgc gatcgcagac
      121 ggtggcgaaa gagtcggtca gcaggatttt tcgtaccggc aactaccact ttcgttgcat
      181 ggctgttcag gtgcgcacga atatatacag caataaccat gtggtaattt gatttttctc
      241 tggaattccg gacacgcaaa ccacgcccat ccgttgctcc gccctctgta tcgtcacagg
      301 gagaattctc gtgaccgcta ccctgccgca agtgccgtcc acagccctgg agtccgtgcc
      361 cggctccgcc tccctgagca gccgctggcg caacctgccg ttgcgtcatc agttccacgc
      421 cctgctgacc atcgtcgtgc tcgcgttgct gctgctggta gccggcacgc tgctgggatt
      481 gcgccgcgat ctcgattcac aggctcgtga agccctggct gcctcgcttc agtccgtgat
      541 gaccgtgctt gagcatttcg ccgacgccga aggcagcgca cggatggacc gtgcgcaggc
      601 acaggcacgt gcgatcgagg cgctgaccgc cttgcgtcag ggcgatacgt atgtgtttgc
      661 ctttgatcgc gatggacgtt tcgtgctgca tggaggcgat cccgcgcagg tcgggcaacc
      721 ggtccggggg ctgcgcgatg ccgagggccg tgacctgtac gaggcttttg caggcgctgc
      781 gcaccgggat gcatccggca gcgggcgggg cgatttcgtg gaatatccgt atgtccgccc
      841 gagcggcacg aagccggagc gcaagctctc gcacgtccgg accttcgagc cctggggctg
      901 ggtgctgggc accggggttt acctgaccga tctggactcc cggttcgcgc gccagcgcaa
      961 cacgctggtg gccggcgtcg ccatcgttgc cgtgctgctc ggcagtctgc tgacctggca
     1021 gagccggcgg atcgtcggac gcgtgcaggc cgtgctcggc tttgcgcagc ggctcgccgt
     1081 ccatgatctg caggcgcaca tgcgcgtcga tacgcaggag gaattcgggc ggatggcagc
     1141 cgcactcaat gccgccgtcg attccttgcg ggaagccgct gcccgcgatc aggcgcagat
     1201 ggagcagctg cgcgcagccg aagccgcccg ggaagtccag tcccgtgaaa tcgccgctca
     1261 ggccgactgc atccgggagg cggccgagcg cgaccgggcc gcagccgatg aactgcgcac
     1321 gaaggtcgga cgcatcgtcg tcgcggtcga ggcggctgcc agcggcgaac tgaccgtcac
     1381 gccgggggtc gggggcaatg acgccatcgg ccgggtcggt gaagccttcg ggcatctgct
     1441 gcacgacctg cgcagcagca ttgccggcat cggccgcaac gccgaggcac tcggacgttc
     1501 ggtggaagtg gcccggaata ccggtcagcg tctgaacggc gtcgctggcg acaccagtgc
     1561 gcgtgccgaa cggctgtccg ggaacgccag tgccgtggat gacagcctgc agtcggtcgc
     1621 cagcgcgacc gaggaaatga atgcagccgt ccaggagatt tcgcgccacg tgcaccgggc
     1681 ggtcgggatt gccgccgtgg ctgcgcaggc cgccgatcac gccaccggtc tcgtcggggc
     1741 cctcacgacc agcagcgccg agatcgggtc ggtcagccgc acgatcaccg gcatcgccga
     1801 acagacgaag ctgctcgccc tgaatgccac gatcgaggcc gcgcgggccg gtgaagccgg
     1861 caagggcttc gccgtcgtcg ccaacgaggt caaggagctt gcgcggggca ccgagcaggc
     1921 gaccgcggac atcgagaagc gcatcggcac gatccagcac gataccggcc atgtcgtgca
     1981 tgcgatcacc gacatccgcc gcaccatcga cgaggtcaac gaaatctccg cgatgatcgc
     2041 cgccgcgatc gaggaacaga gcgccaccac gcgcgagatc agccgcagcg tggcactggc
     2101 tgcgcagggc agcgccgaga tcacggcttt cgtgcgccag ctggccgata ccgcccaggc
     2161 ggtgcacacg gaagcggggg cgacgacgca ggcggccgac cagattgccc gcatcgccga
     2221 ggatcagatc cagctgatca ggcgcttccg catctgaacg caagcgcgtc ctgcccgccg
     2281 gggaccggtc gcggacggtt cccggcctgc ccgagccgtg ccggcctcga tggcaggggt
     2341 ggcaatatgt caaaagatgc gatccgccgg cctggcggaa atgccgggag cggggcgtcg
     2401 gtacgatcgg gccggtgcga caacatgtgt agcaggatgc cgatccatga ccttttctcc
     2461 acgccgcctg ttctggcccc tcgtgctgct ggcgagtgca gtcggcctct gcggcctgct
     2521 cggcatccag tacacgaatg tgcggcagct cgacagcgac gcacacgcat tcgcccgcca
     2581 gatcgagctg ctgcgcaatg ccttcggcta cggcggcttc attcatcatt tcaagaactt
     2641 cctgctgcgg cgcacgcagg cgagctatgc gctcgccggg gccggactcg attcgctcgg
     2701 cgtcacggcg gccgaactcg agcagttcac gcatgatctc gccgagcgcg aggccctgca
     2761 tgaaatcggg cgggtgatcg ggcgctatcg cacggcctgg cagcttgccg gcctgcccac
     2821 gcaccgcgag atgcagcccg aggccctcga cgggctggtg cgggtggaag acaccgtggc
     2881 gatatcggcg ctcggcatcc tcgaggccgc tgcccagcag cgtctcgcgc aggcacgcgc
     2941 ggccagcacg cagctgcaga tgctgctggc agccagtctg gtcgcgctgc ccttgctggc
     3001 actggtgtat ctgcaccagc agcgggcgtt gagacgcgag agccgtctgg ccgactgtct
     3061 ggcgcactcg gaacgggagc ttcgccagag caacgagcag atgcgggcca tcttcgacgg
     3121 catggtccat ctcatctgtc tgctgcgccc ggacggcacg ctgatcgagc tcaaccgcag
     3181 cgcggcgctg ttcgccggcc aggcgcgcga cacgctgctc ggcctgcaac tggccagcct
     3241 gccgtggtgg ccgcgctcgc ccgatgcgca ggcggccgtg cagcgcgcgc ttgccgtggc
     3301 cggcagcggt cagtgcttcc gcgaggagtt cccggtgctc ggaccgggcg gcttccgggc
     3361 gacgctcgat gtttcactgc agccgctgcg tgacgagcac ggcgagatcg tgcggctggt
     3421 gctggaggca cgtgacctga gcgaacgcca gcgtctggag gccggcatcc gcgccgaggg
     3481 cgagcgggca cgtcgctatc tcgacatcgc ccgctcgatc atcctggcgc gcagcatcga
     3541 cgggcgcatc cagctcatca accgcgaagg ctgcgaactg atcggtctgc cggccgggca
     3601 gctcatcggc cggaactggt tcgaaacctg tgtgccgcag ggggagcgca cgagggcgca
     3661 ggtcgtgttc gcccaggtga tcaccggctt tgatccgctc atcgaggagg aacgcgcgct
     3721 gctgaccgcg agcggcgaga cccgctgcat tgcctggcgg cacacggtgg tctgcaacga
     3781 gacgggcgtc gtggtcgcca tcctgagcgc cggacgcgac gtgagcaccg aacgggccgc
     3841 ggagctgcat ctgcgctcga tcaatgtcga gctggagcgg cgcgtccgcg agcggacccg
     3901 ggcgctggaa gatgcccgtg atgcgctgct cgggcgccag caccgcctcg gcatcctgaa
     3961 gacgctggcc gagcaggcgg gtagcgcggc cgatctgtcc gtggtgctga acctgacgct
     4021 gcgcctggtc tgcgaacatc ttgcgtggcc ggtcggccat gcctggattc gcgatgccgt
     4081 cgatcagccg ctgcgctcca gccactgctg gcatctcggg gatccggaaa tctatgccgg
     4141 cttccaggcg cgttcgcagg cgctggaatt cgggccgggc gtcggcgtca tcggccgtgt
     4201 gctcgaggcc ggggaggcgc agtggatcga ggatctggca actgacgtgc atttcctgcg
     4261 tcatgacccc ctggtgcgtt ccggcctctg cggcggcggt ttctgtccgg tgcgggtccg
     4321 cggcgaggtc cggctgctgc tggagttctt ctcgaccgac gataacgggg tgaccgggga
     4381 aatccgggat ttcgtccacc aggtcgcgac gctgctcggc atgatcgccg agaccctgct
     4441 cgatcgcgcc gaactgcgcc ggctcgcgct ctttgccagc gaaaccgacg acatgctcgt
     4501 cgtcaccgat cgcgagggac gcatcgagtg ggtcaaccag ggcttcagcg actgcaccgg
     4561 ctggacgtgc gccgaggcgc tcggacagca ccccggacac ctgatctacg gtccccgcac
     4621 cgacgccgga accatcgccg gcatgcaggc cgcgatgcgg gcaggggaca agatcggcac
     4681 cgaactcgtc gtctaccggc gcagcggcga gcccttctgg gtggcgctga ccgtgcagcc
     4741 ggtatacgac gccgccggcc ggctcgaacg ctacctgtca gtcgcccatg acatcagcca
     4801 gcgtcaccag gccgatgaac tgctgcgcag ccgcgagcag gaactgcgct cgctgttcga
     4861 gaacgtcatc gacggcatca tcacgatgga tgcgaagggg cgcgtccgcg ccttcaatcc
     4921 ggcggccgag cggctgttcg gttatgaagc cgccgaggtg atcgggcaca acgtgtccat
     4981 gctgatgccc gagccgcatc gcagtgcgca tgacggctat ctcgcgcgct atgtcgccgg
     5041 cgggcgggcg gcgatcatcg gcatcggccg cgaggtcgag ggcctgcatc gcgacggccg
     5101 caggctcgag atcgatctgg cggtcaacga gtgcgagctg aacggcgagc gctgcttcat
     5161 cggcatcgtg cgcgacatcg ccgagcgacg gcgcatcacc gccgaactgg agcgggcccg
     5221 cgatgccgcc gagcaggcca accgcgccaa gtcgaccttc ctcgcgacga tgagccacga
     5281 gatccgcacg ccgatgaacg gcgtcatcgg catgctggag gtgctcggtc acgaaacgct
     5341 cacgcccgcc cagaccgaca ccgtggccac gatccgccag tccgcgcaca cgctgctgtc
     5401 gatcatcgac gacgtgctcg acttctcgaa gatcgaggcc ggccaccttg cgctcgagca
     5461 cgcgccgctg ccgctcgcgg ccatcatcga gagcatcggc gacacgctcg cgccgatcgc
     5521 ggccagcaag caggtcacgc tggaaatcta cgtcgatccg cgcctgcccg agtggatcct
     5581 cggggatgcc gtgcgcctgc ggcaggtgct ctacaacctc gccggcaacg ccgtgaagtt
     5641 caccggagag caaacggatc gccccggctg cgtggccttg cgcgccgagt gcggaacggc
     5701 cggcggtctg cgcctgcgcg tcatcgacac cggcatcagc atgtccgcac agaccatcga
     5761 gcgcctgttc cagccgttca tgcaggccga ggcctcgacg acgcgccgct tcggcggcac
     5821 cggtctcggc ctgtcgatcg tggcccgact catcgatctg atggggggca ggatcggcgt
     5881 cgacagcaca cccgggcttg gctcgacgtt cacgatcgag ctgccgcttg aggcagccgg
     5941 cgggcagccg tcgattgcac cgcgctacgc cttcgccggc ctgccggtca gcctgctctg
     6001 cggcggcgac cggctcgggg ccgatctcgg cgaatatctc gccgccgccg gtgcgcgggt
     6061 actgccggcg gccgacgtcg atacgctcgt gcaggacagt gcggcggcag gcgatgcccg
     6121 gctcgcgatc gtggtcgtcg acggtcgctg ccacggcgcc gacagcatcg aacgcctgcg
     6181 cgggcagttc gcggcgcagg cgggccggcg tgacgtgcgc tacgtgctga tcacgcacgg
     6241 caggcggcgc gaaagccgca tcgaaggcga tgacctgatc acgctcgatg gcaacgtgct
     6301 gcaccgccat gtattcctgc gcgcggtggc catcgcggcc ggcctggcct cgccggtcac
     6361 accagcggtc gaaaccatgc tgccgctgcc ggcagctgcg ccgccgggca tcgaagctgc
     6421 ccgcaccgca ggccggctga tcctggtcgc cgaggacaat gcgatcaacc gcaaggtcct
     6481 cgaacgccag ctggcgctgc tcggccatgc cgcggagttt gtcgccgacg gccgcgctgc
     6541 gctctcccgc tggcgcagcc accggcatgc gctgctgatc accgacctgc acatgccgga
     6601 actcgacggc tacgaactcg ccgccacgat ccggcacgag gaaagcgcca gcggcagccg
     6661 gcgccggccg atcatcgcgt tcacggccaa tgcgctgaag ggggaagtcg aacactgtcg
     6721 cagcgtcggc atggacgact gcctgatcaa gccgctgcag atcgagcggc tgcgcacggc
     6781 gctcgaacgc tggctgccgg tcgcgagcgt ccccgccgcc gcggccccgg ttcccgaagc
     6841 gccgccgccc cgttccggcg aacggccgcc cccgttcgat ccgctcgcgc tgcaggaact
     6901 gatcggcgat gacgcggaac tcatcaccgg gctgctcgat gaattcctcg acggcgtccg
     6961 tgcgaatctc gatgcgctgc ggcaactgca ggcagatcaa gacctgacgg gcatcgccga
     7021 tatcggacac agactgaaat cctcggcccg tgccgtcggc gcagccgaac tcgggatgct
     7081 ctatgagcag ctcgaacagg ccgggcgggc cgatgacggg ccggccatcg atgccgtgct
     7141 tgcggcactt gccccggcca tcgcgcgtct gacggcccgg atcgaacccc atgcctggaa
     7201 tccctgcaca tgaacacgga ttgcctgccg gatggcgatg accggaaaca ggatcggaag
     7261 aatgacgcgg aaatcctgct gctcgacgac gagccgctgc tgctgcgcgt cctggcgctg
     7321 gcgctgaacg cctgcggcta tcgcagcatc acgagctgtg cgaacgccgc cgatgcgctg
     7381 ctgcggctcg acgatgccgc actgccgccg atcgaactga tcttctgtga tctcaacatg
     7441 ccgggcatgg acggcgtgca gttcgtgcgc gcgctcgggg agcgcgggtg gcagggcagc
     7501 ctcgtgctga tcagcggcga ggagcggcgc accgtcgaca cggtcgcgcg cctggccagc
     7561 gcccaccggc tcgacctgct ggggcagatg ccgaagcccg tcgtgcgggc gcagctcgtc
     7621 gaactgctcg aacgctggcg cacgcggcag ccggcgcccg gacccggtgc ccaggccggc
     7681 gaggtcgagc ccggggaatt gcgccgggcg ctggcggccg gggagttcgt caacgtctac
     7741 cagccgaaga tggcctttgc cggcggcggc atcgaaggcg tcgaggcact ggtgcgctgg
     7801 cagcatccgg ttgccggcat catctacccg gatcgcttca tcggcgttgc cgagcgtcac
     7861 ggcctgatcg acgcgctgac gcaggcggtg tgcgcccggg ccctggccga tgcgagcgac
     7921 tggcagcgtc aggggctcgg gctgtcggtg gcgatcaacg tctcgatgga cagcctgcgc
     7981 gatctggact ttcccgagcg catcgccgcc gaggtgcgcc gtgccgcctt caacccggcg
     8041 cagctcgtgc tggaactcac cgagagccgg ctgccgccga accccgtcgt gctgctcgat
     8101 acgctgaccc ggctgcgcct gaagcgctac aggctgtcga tcgatgactt cggcaccggc
     8161 tactcgacgc tcgcgcagct gcgcgacatc cccttcgacg agctgaaaat cgaccgcggc
     8221 ttcgtgcacc gcgcgcacgc cgatccggcg cgcggtgcga tcctcgcagc cagcctcggc
     8281 atggcgcacc agctcgggct gcgcacggtg gccgagggca tcgaggatgc tgccgactgg
     8341 cagcacgtgc agcgcctcgg ctgtgatctg gggcagggct atcacatcgc caggccgatg
     8401 ccgccggcgg cgctgccggc atgggtcgcg gcccggagcc tgtccggtca ggcccgctga
     8461 cgcaggcaag gcagcaaggg cgacgggcag gggccatcgg gtcgcgcaag gcaaggcggg
     8521 acggggggcg ggaactgctc gtaaatagat gtttacgtga ccggcttccg gcgttcttat
     8581 gatccgatgc attggcgtgc gccggtccgc ccggtcgcag cgccgcagcg cccgccagag
     8641 gcgcatcccg atccaccggg gggcagagga gacgtgccgg atgaacgcag tcgccgggat
     8701 tcaggggcgc gagcggtatc gccccgtcca caagctccgc ttcgtgaccg ccgcggcgct
     8761 gttcgacggc cacgatgcct cgatcaacct gatgcgccgc atcctgcagg cccagggcac
     8821 cgaggtgatc cacctcggcc acaaccgttc ggtcgagagc gtcgtcagtg ctgcgctcga
     8881 tgaggacgtg cagggcatcg ccgtcagctc ctaccagggc ggccacgtcg agtacttcag
     8941 gtacatgatc gacctgctgc gcgcgcgcgg cggcgggcac atccgcgtct acggcggcgg
     9001 cggcggcgtc atcgtgccgg ccgagatcgc cgagctgcag gattacggcg tgacgcggct
     9061 gttctcgccc gaggacggcc agcggctcgg gctcgccggc atgatcaacc tgatggtcga
     9121 ggcctgcgat gtcgatctcg ccgcgcaggc cccggcgacg ctcgatggac tgcgcgccgg
     9181 cgaccggctc gcgcttgcgc gctggctcag cgtgctcgaa gccgggcgca tggcggcttc
     9241 gcttcgcgac gaactgctgc aggccgcgca ggcccgccgt gcgccggtgc tcggcatcac
     9301 cggcaccggc ggcgccggca agagctcgct gaccgacgaa ctgatccgtc gcttccggct
     9361 cgatcgcgac gaggcgctga agatcgccgt gctcgccgcc gatccggtgc gccggcgctc
     9421 gggcggcgcg ctgctcggcg accgcatccg catgaacgcg atcgagcatc cgaatctcta
     9481 cctgcgctcg ctcgccacgc ccgccggcgg cagcgagctg ccgcagcggc tcgatgagct
     9541 cgtggccgca tgccgggtcg ccggcttcga tctcgtcatc atcgagacgc ccggcatcgg
     9601 tcagggcgcg gccggcatcg ttgcgcacgc cgatctggcg ctgtacgtga tgacgccgga
     9661 atacggcgcc gcctcgcagc tcgaaaagat cgacatgctc gatttcgccg acgtggtcgc
     9721 gatcaacaag tacgaccgcc cgggcggcgc cgacgcgctg cgcgacgtgc gccggcaggt
     9781 gcagcgcaac cgcgaggcct ggggcacgcc gcctgcggat ttgccggtgt tcggcacgat
     9841 tgcctcacgc ttcaacgacg atggcgtcac ggcgctgtac cagcatttgt cggagcggct
     9901 cgccgcgctc ggcctgccgc cggccggcgg gcggctggca gcggtcacga cgcgtgcctc
     9961 gaccggcagc cgcgcgctgc tgccgccggc gcgcgtgcgt catctggccg ccatcgccga
    10021 aaccgtgcgc gcctaccacc gccgcgtcga cgagcaggcc ggactcgtgc gcagcctgca
    10081 gcaggtcgaa gccgtgcagg cgatgctgcg ggcgagcgag gcccggcacc gccatctctt
    10141 cgaagcggcg g
//
LOCUS       DAOQJH010000006         9725 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_3697_length_9725_cov_4.508997, whole genome shotgun sequence.
ACCESSION   DAOQJH010000006 DAOQJH010000000
VERSION     DAOQJH010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 9725)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9725)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9725
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3697_length_9725_cov_4.508997"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..716)
                     /gene="mltG"
                     /locus_tag="PLQ18_00315"
     CDS             complement(<1..716)
                     /gene="mltG"
                     /locus_tag="PLQ18_00315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HNE00118.1"
                     /translation="MKRILAGALLLVLLLGGGAAWLDYRRFLDSTLPVPAGGLDYLVA
                     PGSSVGALAADLQQRGVLRRALWFELYARLHGLAGRIQAGEYRIEPLDTPRALLDRWV
                     AGRVIVRSFTVPEGWTFAQIRAALATLPKVGQTLAGLDDAQVMERLGEPGLAPEGRFF
                     PDTYRYQSGTPDLELLRQMRRALERQLAAAWAKRAPDSPLKTPDEALVLASIVEKETG
                     LPAERRQVAGVYARRLALGMP"
     gene            complement(713..1543)
                     /gene="pabC"
                     /locus_tag="PLQ18_00320"
     CDS             complement(713..1543)
                     /gene="pabC"
                     /locus_tag="PLQ18_00320"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010488918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008696 - 4-amino-4-deoxychorismate lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HNE00119.1"
                     /translation="MSPDSLIDGVPETRLALADRGLQYGDGVFETLAVRAGRPEFWAP
                     HLSRLAAGCERLGLPLPGAGLLRQEADRLCAARDRAVLKIVLTRGCGGRGYRPPQPCT
                     PTRALSLHPWPADAVPLAPVAVRWCAHPLSENPRLAGLKHLNRLDQVLARAEWDDPDL
                     QEGLMCLPDGTPVEATAANLFLVRDGRLHTPRLDRAGIAGIVRGVLIGRARQLGLEVL
                     EARVDRDELLAADELFLCNSVYGLRPVRALGERRWPAPGPMTQYLAEDFETLRRAEAA
                     "
     gene            complement(1540..2880)
                     /locus_tag="PLQ18_00325"
     CDS             complement(1540..2880)
                     /locus_tag="PLQ18_00325"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017357546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HNE00120.1"
                     /translation="MTCLRELPAVPDPLALHRHDPARYPCLLESVARGGGNGRWSLVF
                     AFPQARLQAQPGTDFLDRLDAAWRAERLPAADDADLPEGLPFRGGWFVYLGYELAAEV
                     EPVLHLPAATDGLPRARALRVPAALAIDHACGRAWAVAEAGGEAHVERLLADCAGLPA
                     VSGPAAEPVLAEALVEDDPAGFLDGVARIREYILAGDVFQVNLSRAWRGRLVPDAGAD
                     ELYARLRVANPAPFAGLARFGAQAVVSSSPERLLRVRGRRIDTRPIAGTRRRDAGAVA
                     DAALSAQLLAHPKERAEHVMLIDLERNDLGRVCRPGSVRVDELMAIESYAHVHHIVSN
                     VAGELREDVTPGAAIRAVFPGGTITGCPKVRCMQIIAELEATGRGAYTGAFGYLNRDG
                     DLDLNILIRTLAVDGEHLVLRTGAGIVADSEPAHELAETRHKARGLLLALGAGA"
     gene            complement(2965..4215)
                     /gene="fabF"
                     /locus_tag="PLQ18_00330"
     CDS             complement(2965..4215)
                     /gene="fabF"
                     /locus_tag="PLQ18_00330"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014705222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HNE00121.1"
                     /translation="MGTTGKRRVVVTGLGIVSPVGNTVDEAWGNILAGRSGIAALTAF
                     DTSNFPVRFGGSVRGFSAEPYIPKKDAKKMDLFIQYGVAAAADALADSGLVVTEANAE
                     RIGVAIGSGIGGLPGIEDGHASFLHGGPRKISPFFVPRSIINMISGQVSILQGLKGPN
                     IAVVTACTTGTHNIGEAARIIAYGDADVMIAGGAEMATTPTGLGGFAAARALSTRNDS
                     PEQASRPWDRDRDGFVLSDGAGVLVLEEYEHAKARGARIYCEFVGFGMSSDAYHMTLP
                     MENGDGARRCMLAALRDARLNPGQIDYINAHGTSTPAGDVAESTAVKAALGEHAYRVA
                     VSSTKSMTGHLLGAAGGVEAVFSVLSLRDQVVPPTINLDQPGEGCDLDYVPHTAREMK
                     IDVALSNSFGFGGTNGTVIFRRLT"
     gene            complement(4352..4594)
                     /gene="acpP"
                     /locus_tag="PLQ18_00335"
     CDS             complement(4352..4594)
                     /gene="acpP"
                     /locus_tag="PLQ18_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HNE00122.1"
                     /translation="MSTIEERVKKIIMEQLDVKEEDVTSDASFVDDLGADSLDTVELV
                     MALEEEFECEIPDEDAEKITTVQQAIDYVTANAAGN"
     gene            complement(4760..5503)
                     /gene="fabG"
                     /locus_tag="PLQ18_00340"
     CDS             complement(4760..5503)
                     /gene="fabG"
                     /locus_tag="PLQ18_00340"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005960716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HNE00123.1"
                     /translation="MNLEQRVALVTGASRGIGQAIARELGRRGATVVGTATSESGAAA
                     ISEYLAADGIRGRGLMLNVTDAAAIEAAVKAVTQEFGSLSILVNNAGITRDNLLMRMK
                     DEEWDDIIATNLTSVYRLSKAVIRGMMKARHGRIITVGSVVGSTGNPGQTNYAAAKAG
                     LLGFTKSLAREIGSRGITVNAVAPGFIDTDMTRALPEEARAALVRSIPLERLGRPEDI
                     AHAVAYLASDEAGYVTGETLHVNGGMHMP"
     gene            complement(5509..6456)
                     /gene="fabD"
                     /locus_tag="PLQ18_00345"
     CDS             complement(5509..6456)
                     /gene="fabD"
                     /locus_tag="PLQ18_00345"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HNE00124.1"
                     /translation="MSTSSTLAFAFPGQGSQSVGMLAEAATRPEVRDTFAEAGEALGF
                     DLWQLVQAGPEAELNRTDNTQPAMLAAGVALWRVWCAEGGPQPALLAGHSLGEYTALV
                     AAGALGFADAVRLVAERGRLMQAAVPAGAGAMAALLGLDEPAVREACAEAAQGEVVEA
                     VNLNAPGQIVIAGTATAVARAVEAAKARGAKRAVVLPVSVPSHCALMRPAAERLAERL
                     AAVDLRTPSIPVLHNADVARHAEADAIRAALVRQLHSPVRWIETIEAMAAAGITTVVE
                     CGPGKVLAGMNKRIVKGLAAPTMPDLSALLAVLADQKGA"
     gene            complement(6545..7513)
                     /locus_tag="PLQ18_00350"
     CDS             complement(6545..7513)
                     /locus_tag="PLQ18_00350"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HNE00125.1"
                     /translation="MKHARIIGTGSYLPERVLDNAALEKLVETSDDWIVERTGVRRRH
                     IAAEHETTADLAEHAARAAIAMAGVDPLSIDLIVVATTTPDHVFPSVATQIQERLGCH
                     GCPAFDVQAVCAGFVFALSVADQFVRTGMSRRALVIGAETFSRILDYRDRGTCILFGD
                     GAGAVLLDAAETPGIIATHLHADGRYKELLWVPNGVSSGYDETQKNRAYVSMRGNEVF
                     KFAVNTLGRVAVETLEKNGIDAAELDWLIPHQANLRIIQATARKLGLPMEKVIVTVAE
                     HGNTSAASVPLALDLGVRDGRIRPGQLLLLEAFGGGFTWGSALVRY"
     gene            complement(7510..8538)
                     /gene="plsX"
                     /locus_tag="PLQ18_00355"
     CDS             complement(7510..8538)
                     /gene="plsX"
                     /locus_tag="PLQ18_00355"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011629168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HNE00126.1"
                     /translation="MTRPITIALDAMGGDFGPEVVVPAALDFLEARTDLQLILVGRPD
                     VLEARLAACGGLGRAGLSIHAASEVVGMDEPPAQALKHKKDSSMRVAINLVKSGEADA
                     CVSAGNTGALMATARFVLRTLPGIDRPAICTTLPTVHGHVHMLDLGANADCKSDHLLQ
                     FAVMGAELARAVDGLPAPRVGLLNIGSEDIKGNELVKETHALLGNSDLNYIGYVEGDG
                     VYLSPVDVVVCDGFVGNVALKTSEGVAKLVRHFIGEEFRRSLPNRIAGLIAGPVLRAF
                     AKRIDPRRYNGASLLGLNGIVIKSHGGADRLAFANALRIAAIEVEKNVPALIKHHLGA
                     LHAGGDAT"
     gene            complement(8645..8827)
                     /gene="rpmF"
                     /locus_tag="PLQ18_00360"
     CDS             complement(8645..8827)
                     /gene="rpmF"
                     /locus_tag="PLQ18_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019342480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HNE00127.1"
                     /translation="MAVQQNRKSRSRRDMRRSHDHLEASTLSVDRTSGETHLRHHVSP
                     DGFYRGRRVIAAKAEE"
     gene            complement(8875..9426)
                     /locus_tag="PLQ18_00365"
     CDS             complement(8875..9426)
                     /locus_tag="PLQ18_00365"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceD family protein"
                     /protein_id="HNE00128.1"
                     /translation="MMRALMHARPPDRFDPWKFAVNGGTLAGEWPVATLARFAELLHE
                     PVGSVRWRLAGERDADGRKLVHVWLDAEPRLSCQRCLGPVTLAVQVDALLAPIAAESQ
                     APQLPAQYEPLLAGDEGVSIAELLEDELILALPLVAMHEDERECAAGGYALPPTAGPA
                     PVADGKPNPFAALSTLLKNPKDA"
     gene            9528..>9725
                     /locus_tag="PLQ18_00370"
     CDS             9528..>9725
                     /locus_tag="PLQ18_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HNE00129.1"
                     /translation="MTDACLPDGRRLLLASTSPYRRALLERLRLPFACVAPGTDETPR
                     NGESPRGLVARLSLAKAAAVAQ"
BASE COUNT         1431 a         3429 c         3464 g         1401 t
ORIGIN      
        1 ggcatgccga gcgcgagccg ccgcgcgtag acgccggcca cctgccggcg ttccgcgggc
       61 aggccggttt ccttctcgac gatcgaggcc agcaccagcg cctcgtccgg cgtcttcagc
      121 ggcgaatcgg gcgcgcgctt cgcccaggcg gcggcgagct ggcgctccag cgcccggcgc
      181 atctgccgca gcagctccag gtcgggcgtg ccgctctggt agcggtaggt atccgggaag
      241 aagcggcctt ccggagccag ccccggctcg ccgagacgct ccatgacctg tgcatcgtcg
      301 aggccggcca gcgtctgccc gaccttcggc agcgtcgcga gtgcggcacg gatctgcgcg
      361 aaggtccagc cctcgggcac cgtgaagctg cgcacgatca cgcgcccggc gacccagcga
      421 tcgagcagcg cgcgcggcgt gtccagcggc tcgatgcggt actcgccggc ctggatgcgc
      481 ccggccagcc cgtgcaggcg cgcgtacagc tcgaaccaca gcgcccggcg cagcacgccg
      541 cgctgctgca ggtcggcggc gagcgcaccg accgagctgc cgggagcgac gaggtagtcg
      601 agccccccgg ccggcaccgg cagcgtcgaa tcgagaaagc gccggtagtc gagccaggcc
      661 gccccgccgc cgagcagcag caccagcagc agtgcgcccg ccaggatgcg cttcatgccg
      721 cctccgcccg gcgcagggtc tcgaagtcct cggcaaggta ctgcgtcatc ggccccgggg
      781 ccggccagcg gcgctcgccg agggcgcgca ccggacgcag gccgtagacg ctgttgcaga
      841 ggaacagctc gtcggcggcc agcagttcgt cccgatcgac gcgcgcttcg aggacttcga
      901 ggccaagctg ccgcgcccgc ccgatcagca cgccgcgcac gatgccggcg atgccggcgc
      961 gatcgagccg cggcgtgtgc agacgcccgt cccgcacgag gaagagattg gcggcggtgg
     1021 cctcgaccgg cgtgccgtcc ggcaggcaca tcagcccttc ctggagatcc ggatcgtccc
     1081 actcggcgcg cgcgagcacc tgatcgagcc ggttcaggtg cttgagcccg gcgagccgcg
     1141 ggttttccga cagcggatgc gcacaccagc gcacggcgac cggcgcgagc ggcacggcat
     1201 cggccggcca cggatgcagg ctcagcgcgc gcgtcggcgt gcacggctgc ggtggccgat
     1261 agccgcgccc gccgcagccg cgcgtcagca cgatcttcag caccgcccgg tcccgggcgg
     1321 cgcacagccg gtccgcttcc tgacgcagca gcccggcgcc gggcagcggc aggccgaggc
     1381 gctcgcagcc ggccgcgagc cggctcaggt gaggcgccca gaactcgggc cggccggcgc
     1441 gcacggccag cgtctcgaac acgccatcgc cgtactgcag gccgcggtcg gccagcgcga
     1501 gccgggtttc cggcacgccg tcgatcaggc tgtccggact catgccccgg ccccgagcgc
     1561 gagcagcagg ccgcgtgcct tgtggcgggt ctcggcgagt tcgtgcgccg gctcggagtc
     1621 ggcgacgatg ccggcgccgg tgcgcagcac cagatgctcg ccgtcgacgg cgagcgtgcg
     1681 gatcaggatg ttcaggtcga ggtcgccgtc gcggttcaga tagccgaagg cgccggtgta
     1741 ggcgccgcgg ccggtggctt cgagctcggc gatgatctgc atgcagcgca ccttcgggca
     1801 gccggtgatc gtgccgcccg gaaacaccgc gcggatcgcc gcgcccggcg tcacgtcctc
     1861 gcgcagctcg ccggcgacgt tcgagacgat gtgatgaacg tgcgcgtagc tctcgatcgc
     1921 catcagttca tcgacccgca cgctgcccgg ccggcagacg cggccgagat cgttgcgttc
     1981 gagatcgatc agcatcacgt gttccgcgcg ctccttcgga tgcgcgagca gctgcgccga
     2041 cagtgccgca tcggcgacgg ccccggcatc gcgccggcgc gtgccggcga tcggccgggt
     2101 gtcgatgcgc cggccgcgca cgcgcagcag ccgctccggc gaggaactga ccacggcctg
     2161 cgcgccgaag cgcgccagcc ccgcaaacgg cgccggattg gccacgcgca ggcgcgcgta
     2221 cagctcgtcg gccccggcat cgggcacgag ccgcccgcgc caggcgcgcg acaggttcac
     2281 ctggaacacg tcgccggcga ggatgtactc acggatgcgc gcaacgccat cgagaaaacc
     2341 agccggatcg tcctcgacga gggcttccgc cagcaccggc tccgcggccg gcccgctgac
     2401 ggcaggcagg ccggcgcagt cggcgagcag ccgctcgaca tgcgcctcgc ccccggcctc
     2461 ggccaccgcc caggcacggc cgcaggcgtg gtcgatcgcc agcgccgccg gcacgcgcag
     2521 cgcacgggcg cgcggcaggc catcggtcgc cgccggcagg tgcagcaccg gttcgacctc
     2581 ggcagcgagt tcgtagccga gatacacaaa ccagccgccg cgaaacggaa ggccctcggg
     2641 aagatccgca tcgtccgcag ccggcaggcg ctcggcccgc cacgcggcgt cgagccggtc
     2701 gagaaaatcc gtgcccggct gcgcctgcag ccgggcctgc ggaaacgcga acaccagcga
     2761 ccagcggccg ttgcccccgc cgcgcgcgac gctctccagc aggcagggat agcgcgccgg
     2821 atcatgccgg tgcagggcca gcggatcggg cacggcgggc agttcgcgca ggcaggtcaa
     2881 ggcaggcgga ctcgcaacaa agccgaagcc ggcccggggc ggacccggaa ccggcttcga
     2941 ggggagacga aggacggcga cgactcaggt gagccggcgg aagatcaccg tgccgttggt
     3001 gccgccgaaa ccgaaggagt tcgacagcgc cacgtcgatc ttcatctcgc gcgccgtgtg
     3061 cggcacgtag tcgagatcgc agccctcgcc cggctggtcg aggttgatcg tcggcggcac
     3121 gacctgatcg cgcagcgaca gcaccgagaa caccgcctcg acgccgccgg ccgcgccgag
     3181 caggtgaccg gtcatcgact tcgtcgagct gaccgcgacg cggtacgcat gctcgccgag
     3241 cgcggccttg accgccgtgc tctcggccac gtcaccggcc ggcgtcgagg tgccgtgcgc
     3301 gttgatgtag tcgatctgcc cggggttgag ccgcgcatcg cgcagcgccg ccagcatgca
     3361 gcggcgggcg ccgtcgccgt tttccatcgg cagcgtcatg tggtaggcgt cgctgctcat
     3421 gccgaagccg acgaactcgc agtagatgcg cgcaccgcgc gccttcgcgt gttcgtattc
     3481 ctccagcacc agcacgccgg caccgtcgga cagcacgaag ccgtcgcggt cacgatccca
     3541 gggccggctg gcctgctccg gcgaatcgtt gcgcgtcgac agcgcacgcg cggccgcgaa
     3601 accgccgagt ccggtcggcg tcgtcgccat ctcggcaccg ccggcgatca tcacgtcggc
     3661 atcgccgtag gcgatgatgc gcgcagcctc gccgatgttg tgcgtgccgg tggtgcaggc
     3721 ggtgacgacc gcgatgttcg gacccttcag gccctgcagg atcgacacct ggccggagat
     3781 catgttgatg atgctgcgcg gcacgaagaa cggcgagatc ttgcgcggac cgccgtgcag
     3841 gaaggacgcg tggccgtcct cgatgcccgg caggccgccg atgcccgaac cgatcgcgac
     3901 gccgatgcgc tcggcgttgg cctcggtgac gaccagacct gaatccgcca gcgcatcggc
     3961 cgcagccgcc acgccgtact ggatgaacaa gtccatcttc ttcgcgtcct tcttcgggat
     4021 gtagggctcg gcactgaagc cgcgcaccga accgccgaag cgaaccggaa agttgctggt
     4081 gtcgaatgcg gtcagcgccg cgatgccgct gcgccccgcg aggatgttgc cccacgcctc
     4141 gtccacggtg ttgccgaccg gggaaacgat gcccagtccg gtgacgacga cgcgtctctt
     4201 gcctgtagtg cccaaacagc ggccctcttg cgacccgcgc cgcgtgccgg cagggccggc
     4261 aactgcgtca ggcggtgagg aaaggaatta cggaaggtag agaagccgcg ccgccaccga
     4321 ggcggcgcaa gtgccccgac ccgtcgccag ctcagttgcc ggcggcgttc gcggtcacgt
     4381 agtcgatggc ctgctgcacc gtcgtgatct tctcggcatc ctcgtcgggg atttcgcact
     4441 cgaattcttc ttcgagcgcc atgaccagtt cgacggtatc gagggagtcg gcgccgagat
     4501 catcgacgaa ggaggcgtcg ctggtgacgt cttcctcctt gacgtccagc tgctccatga
     4561 tgattttctt gacgcgctct tcgatggtgc tcattggttt cctcgcagcg gaagttcggg
     4621 gcaaatgccg ccggttattg taagtgaaac gatttgcccg gtccaagcgc cgtcatgtgc
     4681 aatcgcagcg cgacaaaccc ctgcagcatc atgatcttgc acgaaaccga accggcaggc
     4741 cgaagccttg cggcacggct cagggcatgt gcatgccgcc attgacatgc agggtttcgc
     4801 cggtcacgta gccggcttcg tccgaggcca gataggcgac ggcatgcgcg atgtcctcgg
     4861 gccggccgag ccgttcgagc ggaatgctgc gcaccagcgc cgcacgggcc tcctcgggca
     4921 gcgcacgcgt catgtcggta tcgatgaagc cgggcgcgac cgcattgacg gtgatgccgc
     4981 gcgaaccgat ctcgcgcgcc agcgacttcg tgaaaccgag caggccggcc ttggcggcgg
     5041 cgtaattggt ctggcccgga ttgccggtgc tgccgaccac cgaaccgacc gtgatgatgc
     5101 gcccgtgacg ggccttcatc atgccgcgga tcaccgcctt gctgagccgg tacaccgagg
     5161 tcaggttggt ggcgatgatg tcgtcccatt cctcgtcctt catgcgcatc agcaggttgt
     5221 cgcgcgtgat gccggcattg ttgacgagga tggacaggct gccgaactcc tgggtgacgg
     5281 ccttcaccgc cgcctcgatc gcggcggcgt cggtgacgtt gagcatcagg ccgcggccgc
     5341 ggatgccgtc ggcggcgaga tattcgctga tcgccgcggc gccggattca ctggtcgcgg
     5401 tgccgacgac ggtcgcgccg cgccggccga gctcgcgggc gatggcctgg ccgatgccgc
     5461 ggctcgcgcc ggtgacgagt gccacgcgct gttcgaggtt catggggttc aggctccctt
     5521 ctggtcggcg agcaccgcga gcagtgcgct gagatcgggc atggtcgggg cggcgagccc
     5581 cttgacgatg cgcttgttca tgccggcgag caccttgccg gggccgcatt cgacgacggt
     5641 cgtgatgccg gcggcagcca tcgcctcgat cgtctcgatc cagcgcaccg ggctgtgcag
     5701 ctggcgcacg agggcggcgc ggatcgcgtc cgcttcggcg tggcgggcga catcggcgtt
     5761 gtgcagcacc ggaatcgacg gcgtgcgcag gtcgaccgca gcgagacgtt cggccagacg
     5821 ctcggcggcc gggcgcatca gcgcacagtg cgagggcacg ctgaccggca gcacgacggc
     5881 gcgcttggca ccgcgcgcct tcgccgcctc gaccgcgcgt gcgaccgctg tcgcggtgcc
     5941 ggcgatgacg atctggccgg gggcgttgag gttcacggcc tcgaccacct cgccctgtgc
     6001 cgcttcggcg caggcctcgc gcacggccgg ctcgtcgagc ccgagcagcg cggccatcgc
     6061 gccggccccg gccggcaccg cggcctgcat cagccggccg cgctcggcca cgaggcgcac
     6121 ggcatcggcg aatccgagcg cgccggcggc gaccagcgcc gtgtactcgc cgagcgaatg
     6181 accggcgagc agtgccggct gcggaccgcc ttcggcgcac cagacgcgcc agagcgccac
     6241 gccggcggcc agcatcgccg gctgcgtgtt gtcggtgcgg ttcagctcgg cctcggggcc
     6301 ggcctgcacc agctgccaga gatcgaagcc gagcgcctcg ccggcctcgg cgaaggtgtc
     6361 gcgcacctcg ggccgggtgg cggcttcggc gagcatgccg accgactgcg aaccctgccc
     6421 cggaaacgcg aaggcgagcg tggacgaagt gctcatgcgc gggactccct gctggtgacg
     6481 ccgccgcacg cagccggatg ccgggcagcg gcggacgcct ggatggacat ggatggccgg
     6541 cggctcagta gcggaccagc gccgaacccc aggtgaagcc gccgccgaag gcttcgagca
     6601 gcagcagctg accggggcgg atgcggccgt cgcgcacgcc gagatcgagc gcgagcggca
     6661 ccgaggccgc cgaggtgttg ccgtgctcgg cgaccgtgac gatgaccttc tccatcggca
     6721 ggccgagctt gcgcgcggtc gcctggatga tgcgcaggtt cgcctggtgc gggatcagcc
     6781 agtcgagttc ggccgcgtcg atgccgttct tttcgagggt ctcgacggca acgcgcccga
     6841 gcgtgttgac ggcgaacttg aacacctcgt tgccgcgcat cgacacgtag gcgcggttct
     6901 tctgcgtctc gtcgtagccg ctggagacgc cgttcggcac ccacagcagt tccttgtagc
     6961 gaccgtcggc gtgcaggtgc gtggcgatga tgcccggggt ctcggcggcg tcgagcagca
     7021 ccgcgccggc accgtcgccg aacaggatgc aggtgccgcg atcgcggtag tcgaggatgc
     7081 gcgagaaggt ctcggccccg atcaccagcg cccgccggct catgccggtg cgcacgaact
     7141 ggtcggccac cgacagcgcg aacacgaaac cggcgcagac cgcctgcacg tcgaaggccg
     7201 ggcagccgtg gcagccgagg cgctcctgga tctgggtcgc gacgctcggg aagacgtgat
     7261 ccggcgtcgt ggtcgcgacg acgatcaggt cgatcgacag cggatcgaca ccggccatcg
     7321 cgatcgcggc gcgcgcggca tgctcggcga gatcggccgt ggtctcgtgc tcggcggcga
     7381 tgtggcgccg gcgcacgccg gtgcgctcga cgatccagtc gtcgctggtc tcgaccagct
     7441 tttcgagcgc ggcgttgtcg agcacgcgct cgggcaggta gctgccggtg ccgatgatcc
     7501 gggcatgctt cacgtcgcgt cccctccggc atgcagggcg ccgaggtgat gcttgatcag
     7561 cgccgggacg ttcttctcga cctcgatcgc cgcgatgcgc agcgcattgg cgaaggccag
     7621 ccggtcggcg ccgccatggc tcttgatgac gatgccgttg aggccgagca ggcttgcacc
     7681 attgtagcgg cgcgggtcga tacgtttcgc gaaggcccgc aataccggcc cggcgatcag
     7741 cccggcgatc ctgttcggca ggctgcggcg gaattcctcg ccgatgaagt gacgcaccag
     7801 cttggcgacg ccctcgctgg tcttcagcgc gacgttgccg acgaagccgt cgcagaccac
     7861 gacatcgacc ggactcaggt agacgccgtc accctcgacg tagccgatgt agttgagatc
     7921 ggaattgccg agcagcgcat gggtttcctt gaccagttcg ttgcccttga tgtcctcgga
     7981 gccgatgttc agcagcccga cgcgcggcgc cggcaggccg tcgacggcgc gggcgagttc
     8041 cgcgcccatc accgcgaact gcagcaggtg atcggacttg cagtcggcgt tggcgccgag
     8101 atcgagcatg tgcacgtgac cgtgcacggt cggcagcgtc gtgcagatcg ccggccggtc
     8161 gatgccgggc agcgtgcgca gcacgaagcg tgcggtcgcc atcagcgcgc cggtgttgcc
     8221 ggcactgacg caggcgtcgg cctcgccgct cttgacgagg ttgatcgcca cgcgcatcga
     8281 ggagtccttc ttgtgcttca gcgcctgcgc cggcggctcg tccatgccga cgacctcgct
     8341 cgcggcatgg atgctcaggc cggcacggcc gagtccgccg caggcggcaa gacgggcttc
     8401 gagcacgtcg ggacggccga cgaggatcag ctgcagatcc gtgcgcgctt ccagaaaatc
     8461 gagtgctgcg gggacgacga cctcgggtcc gaagtcgccg cccatcgcgt cgagcgcgat
     8521 cgtgatcggt cgggtcattt cggctgcttt ccgtgacaca cgcggcgcac acgtgcggcc
     8581 ataacgacga agcctccgct agggaggctc gtctgcgctc gggccggcat ggtgccggcc
     8641 ggtctcactc ttcagccttg gcggcgatca cgcgacggcc gcgatagaag ccgtccgggg
     8701 acacgtggtg acgcaggtgg gtctcgccgc tggtgcggtc gaccgacagc gtgctggctt
     8761 cgaggtggtc gtgggagcgg cgcatgtcgc gacgcgaacg ggacttgcgg ttctgctgga
     8821 cggccatgat cgggactcct gattgggtgc cggaggtttc cggtggaagg acgctcaggc
     8881 gtccttcggg tttttcaaca gggtcgacag cgccgcgaac gggttcggtt tcccgtccgc
     8941 gaccggtgcc ggcccggcag tcggcggcaa ggcgtagccg ccggctgcgc actcgcgttc
     9001 atcttcatgc atcgcgacca gcggcagcgc gaggatcagt tcatcctcca gcagttccgc
     9061 gatcgacacg ccctcgtcgc cggcgagcag cggctcgtac tgcgccggca gctggggggc
     9121 ctgcgactcg gcggcgatcg gcgcgagcag ggcatcgacc tgcacggcca gcgtcaccgg
     9181 ccccaggcag cgctggcagc tcagccgggg ttcggcatcg agccacacat gcaccagctt
     9241 gcggccatcg gcatcacgct cgccggcgag tcgccagcgc acgctgccaa cgggttcgtg
     9301 caggagttcc gcgaagcgcg ccagcgtcgc caccggccac tcgcccgcga gcgtcccgcc
     9361 gttgacggcg aacttccacg gatcgaagcg gtctgggggg cgggcatgca taagggcgcg
     9421 catcatatgg gttcaccccg ggcaagtcaa aacgcgccgg cccgttgccg cgcggccccg
     9481 ccgctcccta cgctggggcc tctgccctgc cgcgaggcct gattccgatg accgatgcct
     9541 gcctgcccga cggccggcgc ctgctgctcg cctcgacctc gccgtaccgc cgcgcgctgc
     9601 tcgaacgcct gcgcctgccc ttcgcctgcg tcgctcccgg caccgacgaa accccgcgca
     9661 acggcgaatc gccgcgcggg ctggtcgcgc gcctgtcgct cgccaaggcg gcggcggtcg
     9721 cgcag
//
LOCUS       DAOQJH010000007         9595 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_3767_length_9595_cov_4.533543, whole genome shotgun sequence.
ACCESSION   DAOQJH010000007 DAOQJH010000000
VERSION     DAOQJH010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 9595)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9595)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9595
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3767_length_9595_cov_4.533543"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..835
                     /locus_tag="PLQ18_00375"
     CDS             <1..835
                     /locus_tag="PLQ18_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HNE00130.1"
                     /translation="EAFMGYLLPWGQMSYWGAQVIINLFGAVPFIGPDLSEWIRGDFV
                     VSDATLNRFFSLHVIAVPLVLVGLVAAHIIALHEVGSNNPDGVEIKKLKDENGIPLDG
                     IPFHPYYSVKDLVGVGFFLIFFCAVIFFLPEMGGYFLEHPNFDPADPLKTPPHIAPVW
                     YFTPFYAILRAVPSMAGSAFWGVVAMGASIVLMFFLPWLDRSPVKSIRYRGPLFKGAV
                     ALFVVAFLILGYLGALPTTPGRTLVAQICSAIYFAFFLLMPIYTSIDKTKPVPERVTF
                     K"
     gene            832..1572
                     /locus_tag="PLQ18_00380"
     CDS             832..1572
                     /locus_tag="PLQ18_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005526845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HNE00131.1"
                     /translation="MRKWILAIVLAALPGFALASSEGAALDKANIDPTDKASLQRGAK
                     LFVNYCMGCHSADYQRYNRLARDIGLSDEQVRDNLIFTGAKVGEGMRNAMPKKDAKKW
                     FGAAPPDLTLIARARGVDYLYTYLRTFYIDEARPWGVNNAAFPLVGMPHVLSELQGWQ
                     KARMETHKDAHGNESTVITGYEIVKKGSMSPPEFDSAMRDLVNFLAYVAEPVKLYRQS
                     LGVWVIAFLVLMTAVFYLLKKEYWKDIH"
     gene            1659..2327
                     /locus_tag="PLQ18_00385"
     CDS             1659..2327
                     /locus_tag="PLQ18_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017366284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HNE00132.1"
                     /translation="MAGAASRRSTLTLYSLCNCCHCHRVRLVLLEKGLDPEFVWVNPA
                     SPPPDLLEASPGGHLPTLVDRDVVLYNDRVIIEYLDERYPHPPMLATDPAGRARQRLA
                     LARIEQDLYAQLPRLSRSVREERELAREALRSQLVAGAAVFAARPYFLGTDYSLIDAT
                     LAPLLWRLAHYGIELPPEAAPVLEYAARMFARPAFRQSLSAVEQEMRDDGPAARPPSS
                     ETVR"
     gene            2324..2710
                     /locus_tag="PLQ18_00390"
     CDS             2324..2710
                     /locus_tag="PLQ18_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HNE00133.1"
                     /translation="MTSNRPYLIRALYEWILDNAMTPHILVNARVEGVDVPPQHVHDG
                     RIVLNVSPSAVHGLAMGNDHIEFSARFGGLARTVRVPVVAVMAIYARENGQGTAFPDE
                     PGSEPPPSPSQPERGSGKRPALKLVK"
     gene            complement(2773..3372)
                     /locus_tag="PLQ18_00395"
     CDS             complement(2773..3372)
                     /locus_tag="PLQ18_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008291186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HNE00134.1"
                     /translation="MIRRTLHLLPVLGLSGLVFLGGPSGCAGVVAGGAAAVSVAHDRR
                     TAGTVVEDESIEMKAVAELDRHGISARSHLNVTSYNRSVLICGEVPDEATRQQAEAVV
                     RRVAEVRQVFNEAAILPASTLASRSNDTWLTTRAKTALFNVDGIRDFDPTRVKVVTER
                     GIVYLMGLVSSAEAESVTAAVRSVPGVQKVVRLFEYIDR"
     gene            complement(3409..3714)
                     /locus_tag="PLQ18_00400"
     CDS             complement(3409..3714)
                     /locus_tag="PLQ18_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008929130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HNE00135.1"
                     /translation="MQQGLKPLMRNFRCRPGEIDLVMHETGTLVFVEVRFRRDCGFGT
                     ALESVTAGKQRRLLRAAAVYLQQHGDTLPCRFDVVGISPGPDGHPVYEWVRDAFRAD"
     gene            3829..4695
                     /gene="rsmI"
                     /locus_tag="PLQ18_00405"
     CDS             3829..4695
                     /gene="rsmI"
                     /locus_tag="PLQ18_00405"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012699961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HNE00136.1"
                     /translation="MSVRIGILYVVATPIGNLLDLSPRAQQTLASVERVAAEDTRHSG
                     ALLRHFGIHTPLVSVHEHNERDRCAGLVGELLAGQSLALVSDAGTPLISDPGYHLVRA
                     AQAAGIEVVAVPGPSSLIAALSVSGLPTDRFVFEGFLPARAAARRARLSELAAETRTL
                     VLFESSHRIAESLADLAETFGPAREAAVARELTKRYESVRRAGLGALAAHFAASEEER
                     RGEFVVLVQGAEAPDAGAGADAGAERVLRLLLAELPLGRAVALAAKITGARRNVLYAR
                     ALELSGDGASGA"
     gene            4735..5091
                     /gene="rnpB"
                     /locus_tag="PLQ18_00410"
     ncRNA           4735..5091
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PLQ18_00410"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            5426..5881
                     /gene="mraZ"
                     /locus_tag="PLQ18_00415"
     CDS             5426..5881
                     /gene="mraZ"
                     /locus_tag="PLQ18_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015259365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HNE00137.1"
                     /translation="MFRGISPLSLDSKGRLSIPARFRDRLSESGSDQVVVTIDRDGCL
                     LLYPLPVWEQVEARLVQLSSTNRAARSLKRLLMGHAEELQPDGSGRLLIPAPLREFAK
                     LEKHVVLVGQGNKFELWDEQTWYRLREQWLAEDGGEAGSLPDDLESLSF"
     gene            5886..6821
                     /gene="rsmH"
                     /locus_tag="PLQ18_00420"
     CDS             5886..6821
                     /gene="rsmH"
                     /locus_tag="PLQ18_00420"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008930968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HNE00138.1"
                     /translation="MTSTLLHQSVLLHEAVDALNVRARGCYLDATFGRGGHARAVLAR
                     LGADGCLLALDRDPEAAAEARALAAIDPRLQFVAGPFSGLAAAVRAHGLEGRLDGILF
                     DLGVSSPQLDDARRGFSFQQDGPLDMRMDPAGGPSAAEWLAAAGEAEISLVLRDLGEE
                     RFHRRIARRVVETRAETPIASTAQLAGLVAAAVPTRERGKHPATRAFQAIRIHVNREL
                     DELQDALAQAVGLLAPGGRLAVISFHSLEDRIVKRFLRAGARGQELPPGLPVTGGPSG
                     ATLKLIGKAVQPSAAEIDANPRARSAVLRVAEKLP"
     gene            6818..7231
                     /gene="ftsL"
                     /locus_tag="PLQ18_00425"
     CDS             6818..7231
                     /gene="ftsL"
                     /locus_tag="PLQ18_00425"
                     /inference="COORDINATES: protein motif:HMM:NF016860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HNE00139.1"
                     /translation="MNAPLPLLTTEVTVAPVEVAVPPPVAETEKPQAAEAGRLVDEPA
                     EHDPVGQNLGTVLVLVLTILATGVGIVWSKHQSRKLFVELQGLQKVRDDLDIEWNQLQ
                     LEQSTLATESVIDQAARTRLDMGIPAPEAVIYVIR"
     gene            7228..9021
                     /locus_tag="PLQ18_00430"
     CDS             7228..9021
                     /locus_tag="PLQ18_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014778717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HNE00140.1"
                     /translation="MKRSPSSPSRKLRASPGNNWTTNRRKLVLGLFALAALGIVARAV
                     YLQLIDTEFLQGQGDERFLRSVEVPAHRGMITDRHGEPLAVSSPVDSVWAHPPTLLAN
                     RERLPELARLLGMTPSELEAKVLSREKKEFMYLRRALEPDAAARIQVRAIPGVFLQRE
                     YRRFYPSAEVTSHLLGFTDIDDDGQEGIELAWNSTLQGVPGEKRVIRDRYGHTVDEVE
                     SVREPQPGRTVTLSIDRRIQYIAYRAVKAAMLEHGAAAATAVVLDVQTGEVLAMVNQP
                     GTNPNDRGAIRGDLLRNRALTDVYEPGSVMKPFTVAMGLESGRFRPDTPISTSPGHMS
                     VGRYTVRDTHNYGLIDVTRVIVKSSNVGATKIALALPAERLWDLFRKLGFGRTSGIGF
                     PGEQGGWLAHYSRWSTISHANQSFGYGISMTPLQLARAYAALASGGVIRPVSLVKLDR
                     APEGERILSEATVRKLMPMMEAVVSRDGTAIQASVAGYRVAGKTGTVHKIVGRGYAKN
                     NYYSVFAGFAPVSKPRLAMVVIVDDPKGKSYYGGLVAAPVFSKVMAGALRVMNVPPDN
                     IVDPRLTPARPVMASTTAPAAAPATAKRERP"
     gene            9021..>9595
                     /locus_tag="PLQ18_00435"
     CDS             9021..>9595
                     /locus_tag="PLQ18_00435"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HNE00141.1"
                     /translation="MLPLSVLLAGFARDVPELPVTALALDSRAVTPGAVFCAVRGHSG
                     HGLDHVQQAIARGASAVLWEPPAAAPLLPVPAIPIERLGRQVSQLASRLYGIPSHALA
                     VLGVTGTDGKTSCTHFLAQALSEPGRPAGLLGTLGYGLWGGPFENASHTTPDAIGVQQ
                     RLAAFVEAGVRHAAMEVSSHALDQGRVEAVH"
BASE COUNT         1460 a         3347 c         3303 g         1485 t
ORIGIN      
        1 cgaggccttc atgggctacc tgctgccctg gggccagatg tcgtactggg gcgcgcaggt
       61 catcatcaac ctgttcggtg ccgtgccctt catcggcccg gatctctccg aatggatccg
      121 cggtgacttc gtcgtctccg acgcgacgct gaaccgcttc ttctcgctgc acgtgatcgc
      181 ggtgccgctg gtgctggtcg gcctggtcgc cgcgcacatc atcgcgctgc acgaagtcgg
      241 ctcgaacaac cccgatggcg tcgagatcaa gaagctcaag gacgagaacg gcatcccgct
      301 cgacggcatc ccgttccacc cgtactactc ggtcaaggat ctggtcggcg tcggcttctt
      361 cctgatcttc ttctgcgccg tgatcttctt cctgccggag atgggcggct acttcctgga
      421 gcacccgaac ttcgatccgg ccgatccgct gaagaccccg ccgcacatcg cgccggtgtg
      481 gtacttcacg cccttctacg caatcctgcg cgccgtgccg tcgatggccg gctcggcctt
      541 ctggggcgtg gtcgcgatgg gcgcctcgat cgtgctgatg ttcttcctgc cctggctcga
      601 ccgcagcccg gtcaagtcga tccgctaccg cggcccgctg ttcaagggcg ccgtcgcgct
      661 gttcgtcgtc gccttcctga tcctcggcta tctcggcgcg ctgccgacca cgccgggccg
      721 cacgctggtc gcgcagatct gctcggcgat ctacttcgcg ttcttcctgc tgatgccgat
      781 ctacacgtcg atcgacaaga ccaagccggt tccggaaagg gtgacgttca aatgagaaag
      841 tggattctgg ccatcgtcct cgccgcgctg cccggcttcg cactggcctc cagcgaaggc
      901 gccgcactcg acaaggcgaa catcgacccg accgacaagg cctcgctgca gcgcggtgcg
      961 aagctgttcg tcaattactg catgggctgc cactcggcgg actaccagcg ctacaaccgg
     1021 ctggcgcgcg acatcggcct gagcgacgag caggtcaggg acaacctgat cttcaccggt
     1081 gccaaggtcg gcgaaggcat gcgcaacgcc atgcccaaga aggacgcgaa gaagtggttc
     1141 ggtgcggcgc cgcccgacct gaccctgatc gcgcgcgcgc gcggggtgga ctacctgtac
     1201 acctatctgc gcacgttcta catcgacgag gcgcggccct ggggcgtcaa caacgccgcc
     1261 ttcccgctgg tcggcatgcc gcacgtgctg agcgaactgc agggctggca gaaggccagg
     1321 atggaaacgc acaaggatgc ccacggcaac gagtcgacgg tcatcaccgg ctacgagatc
     1381 gtcaagaagg gcagcatgag tccgccggag ttcgactcgg cgatgcgtga cctggtcaac
     1441 ttcctcgcct acgtcgccga accggtcaag ctgtaccggc agtcgctcgg cgtgtgggtc
     1501 atcgcgtttc tggtcctgat gacggcggtc ttctaccttc tgaagaagga atactggaag
     1561 gacatccact gacgcacggc gcgcggggcg gattgccggt catggcagcc gccccgcgtc
     1621 ccgcgtcccg catcccgccg atctatccgg agagggccat ggccggagca gccagccgca
     1681 gatcgaccct gacgctgtat tcgctctgca actgctgcca ctgccaccgc gtgcgtctgg
     1741 tgctgctgga aaaggggctg gacccggagt tcgtctgggt caaccccgcc agcccgcccc
     1801 ccgacctgct cgaagccagc cccggcggcc atctgccgac gctggtcgat cgcgatgtcg
     1861 tgctctacaa cgaccgcgtc atcatcgaat acctcgacga gcgctatccg catccgccca
     1921 tgctcgcgac cgacccggcc ggtcgtgccc gccagcggct ggcgctcgcc cgcatcgaac
     1981 aggatctgta cgcgcaactg ccgcggctgt cgcgcagcgt gcgcgaggag cgcgagctgg
     2041 cgcgcgaggc gctgcgttcg cagctcgtgg ccggcgccgc ggtgttcgcg gcgcgcccgt
     2101 acttcctcgg caccgactac agcctgatcg acgcgacgct ggcgccgctg ctgtggcgcc
     2161 tcgcgcacta cggcatcgaa ctgccgcccg aggcggcgcc ggtgctcgaa tacgcggcgc
     2221 gcatgttcgc gcggccggcg tttcgccaga gtctgagtgc ggtcgagcag gaaatgcgcg
     2281 acgatggtcc cgccgcccgc cccccgtcca gcgagaccgt gcgatgacct ccaatcgccc
     2341 ctatctgatc cgggccttgt acgaatggat cctcgacaac gccatgaccc cgcacatcct
     2401 cgtcaacgcc agggtcgagg gggtcgacgt gccgccgcag cacgtgcatg acggtcgcat
     2461 cgtgctgaac gtcagcccga gcgccgtgca tggcctggcg atgggcaacg accacatcga
     2521 gttcagtgcc cgcttcggcg ggctggcgcg caccgtgcgc gtgccggtgg tggcggtgat
     2581 ggcgatctac gcgcgcgaga acggtcaggg cacggccttc ccggacgagc cgggcagcga
     2641 accgccgccg tcgccgtccc agcccgagcg tggcagcggc aagcgcccgg cgctgaagct
     2701 cgtcaagtaa ttcgtcaagc aattccgggc aggcaggcgc ccgttcagcc gcgcgcccgg
     2761 ttcccgggcg gatcaacggt cgatgtactc gaacagccgc acgaccttct gcacgcccgg
     2821 cacgctgcgc accgctgccg tgaccgactc ggcttcggcg ctgctcacca gccccatcag
     2881 atagacgatg ccgcgttcgg tcacgacctt gacccgggtc gggtcgaagt cgcggatgcc
     2941 gtcgacgttg aacagcgccg tcttcgcgcg cgttgtcagc caggtgtcgt tcgagcggct
     3001 ggcgagcgtg ctcgccggca ggatggcagc ctcgttgaac acctgccgca cctcggcgac
     3061 gcggcgcacg acggcctcgg cctgctggcg cgtggcctcg tcgggcacct cgccgcagat
     3121 cagcacgctg cggttgtagc tcgtgacgtt caggtgcgag cgggcactga tgccgtgacg
     3181 gtcgagttcg gctaccgctt tcatctcgat gctctcgtcc tcgacgacgg tgccggccgt
     3241 gcgccggtcg tgcgcgacgc tgaccgcggc cgcgccaccg gcgacgacgc cggcgcagcc
     3301 gctcgggccg ccgaggaaga caaggccgct caggccgagc acggggagca ggtgcagggt
     3361 tcggcgaatc atgtgtgcgg gattccgtag gcaggcgggt acgcggtttc agtcggcgcg
     3421 aaaggcgtcg cgtacccatt cgtaaacggg atggccgtcc gggccgggcg agatgccgac
     3481 cacgtcgaag cggcacggca gcgtatcgcc gtgctgttgc aggtagactg ccgcggcccg
     3541 cagcaggcgg cgctgcttgc cggccgtgac gctttcgagc gccgtgccga agccgcaatc
     3601 gcggcggaag cgcacctcga cgaacacgag ggtgccggtc tcgtgcatca cgagatcgat
     3661 ctcgccgggc cggcagcgga aattacgcat caacggtttc aggccctgct gcaccagcca
     3721 ggcttcggcc aaggcctcgc cggccgcgcc gcgcagcaga tgggcaggcc ggccggcgcc
     3781 ggaggtcatg gcgccggagc cctgccacac atcttccgga gtcctgtcgt gtccgtacgc
     3841 atcggcatct tatacgtggt ggccacgccg atcggcaatc tgctcgatct gagtccgcgc
     3901 gcgcagcaga cgctcgcgtc ggtcgagcgg gtggcggccg aggatacccg ccacagcggt
     3961 gcgctgctgc gccatttcgg catccatacg ccgctggtgt cggtgcacga gcacaacgag
     4021 cgcgaccgct gcgccggact ggtcggggag ctgctcgccg gccagtcgct ggcgctggtg
     4081 tccgatgccg gcacgccgct gatttccgat ccgggctatc acctcgtgcg tgccgcgcag
     4141 gcggccggca tcgaggtggt cgccgtgccc ggtccgagca gcctgatcgc tgcgctgtcg
     4201 gtctcggggc tgccgaccga tcgcttcgtg ttcgaaggtt tcctgccggc ccgagccgcg
     4261 gcccgccgtg cacggctgtc ggaactggcg gccgagacgc gcacgctggt gctgttcgaa
     4321 tccagccacc gcatcgccga gagtctcgcc gatctcgccg aaaccttcgg gccggcgcgc
     4381 gaggcggccg tcgcccgcga gctgaccaag cgctacgaat ccgtgcgccg ggccgggctc
     4441 ggggcgctgg ccgcgcattt cgcagcgagc gaggaggagc gccgcggcga gttcgtcgtg
     4501 ctcgtgcagg gcgccgaggc gcccgatgcc ggggccggag cggatgccgg ggccgagcgc
     4561 gtactgcgcc tgctgctcgc cgagctgccg ctcgggcgcg ccgtggcgct ggcggcgaag
     4621 atcaccggtg cccgcaggaa cgtgctgtac gcacgggcgc tggagctgtc cggcgacggg
     4681 gcatccggcg catgacttgc cctgccgtcg gtggccgcgc agaatccgcg cccggaagtc
     4741 ggccggacga tcgcggcccc cgcgtgcggg ggcggaggaa agtccgggct ccgcagggca
     4801 gggtgccagg taacgcctgg ggggcgtgag cccacggaaa gtgccacaga aaacaaaccg
     4861 cccaagtcgc cgcgaggcga acggcaaggg tgaaaaggtg cggtaagagc gcaccgcggc
     4921 gctggcaaca gtcgccggca cggaaaaccc cacccggagc aagaccaaat aggggagcgc
     4981 cagcgtgtgg cccgcacggc tcccgggtag gtcgctacag gcgtccggtg acgggcgtcg
     5041 cagatgaatg atcgtcctcg acagaacccg gcttacaggc cgacttcctc cttcctgaat
     5101 ctccctgttt ccagccccgg cttgcggttc gccgcaagcc gcgcggctgt ctgtccgtgc
     5161 ggcgggggac atgacgtttt ccggcggtgg ctgacagcgg tttgctcctg aatggtgcaa
     5221 tgcggcatcg gcagtccggt ggctgttgtg ggggcgcaac ccatgttgta tgcgcaccga
     5281 taagctgttg tagaaaaagc aaaaccgtgg cttccgcggg ccgtgttctc cttgacaggc
     5341 ttccggcgcg gttcctatag tgggaaaacg tggaacgtag tgggacagtc gggcaggccg
     5401 cggtacggca gcgaggatca ggcgcttgtt tcgcgggatt tcccctcttt cgctcgacag
     5461 caagggacgg ctctcgatcc ccgcccggtt ccgggatcgg ctctccgaaa gtggcagtga
     5521 ccaggtggtc gtcacgatcg atcgtgacgg gtgtctcctt ttgtatccgc tgccggtctg
     5581 ggagcaggtg gaagcgcgac tggtgcagct gtcgagtacg aaccgcgccg cccgcagcct
     5641 gaagcggttg ctgatggggc acgccgagga actgcaaccc gacggctcgg ggcgtctgct
     5701 gataccggcg ccgctgcggg aattcgcgaa gctcgaaaaa catgtggtgc tggtggggca
     5761 ggggaacaag ttcgagctct gggacgagca gacctggtat cggctgcgcg agcagtggct
     5821 ggccgaggac ggcggcgagg caggctccct gcccgacgat ctggaatcgc tctccttctg
     5881 aagtcatgac ctccaccctg cttcatcaaa gcgtgctgct gcacgaggcc gtcgatgccc
     5941 tgaacgtgcg ggcacggggc tgctatctcg atgccacctt cggccgcggc ggtcacgccc
     6001 gcgcggtgct ggcgcgcctc ggggccgacg gctgcctgct ggccctcgat cgcgatccgg
     6061 aagcggccgc cgaggcgcgt gcgctcgcgg ccatcgatcc ccgcctgcag ttcgtggccg
     6121 gtccgttcag cgggctggcg gcggccgtgc gggcgcacgg tctcgaaggc cggctcgacg
     6181 gcatactgtt cgatctcggc gtctcgtcgc cgcaactcga cgatgcgcgc cgcggcttca
     6241 gcttccagca ggacgggccg ctcgacatgc gcatggatcc ggccggcggg ccgagtgcag
     6301 ccgaatggct ggcggcggcc ggcgaggcgg agatttccct ggtgctgcgc gatctcggcg
     6361 aggagcgttt tcaccggcgc atcgcccggc gcgtcgtcga gacccgcgcc gaaaccccga
     6421 tcgccagcac cgcgcagctt gccggcctcg tggccgccgc cgtgccgacg cgcgagcgcg
     6481 gcaagcatcc ggcgacgcgc gccttccagg cgatccgcat ccacgtcaac cgcgaactcg
     6541 acgaattgca ggacgcactc gcgcaggccg tcgggctgct cgcgcccggc ggccggctcg
     6601 ccgtgatcag cttccactcg ctggaagacc gcatcgtcaa gcgcttcctg cgcgccggcg
     6661 cgcgcggcca ggaactgccg cccgggctgc cggtgaccgg cgggccgagc ggcgcgacgc
     6721 tgaagctcat cggcaaggcc gtgcagccgt ccgcggccga gatcgacgcc aatccgcgcg
     6781 cgcgcagcgc cgtgctgcgc gtcgccgaga agctgccatg aacgcgccgc tgccgctcct
     6841 gacgaccgag gtcacggtcg ctcccgtcga agtcgcggtg ccgccgccgg ttgccgagac
     6901 cgaaaagccg caggctgccg aggccgggcg actcgtcgac gagccggccg agcacgaccc
     6961 ggtcggccag aacctcggca cggtgctggt gctggtgctg acgatcctcg ccaccggtgt
     7021 cggcatcgtc tggagcaagc accagagccg caagctgttc gtcgagctgc aggggctgca
     7081 gaaggtccgc gacgacctcg acatcgaatg gaaccagctg cagctggagc agagcacgct
     7141 cgcgaccgag tcggtgatcg accaggccgc gcgcacccgg ctggacatgg gcatacccgc
     7201 gcccgaagcg gtcatctacg tgatccgatg aaacgctctc cgtcatcgcc gtcgcgcaag
     7261 ctgcgcgcgt ccccgggcaa caactggacg accaatcgcc gcaagctcgt cctcggcctg
     7321 ttcgcgctgg ccgcgctcgg catcgtcgcg cgcgccgtct acctgcagct gatcgatacc
     7381 gaattcctgc agggccaggg cgacgagcgc ttcctgcgca gcgtcgaggt gccggcacac
     7441 cgcggcatga tcaccgaccg ccacggcgag ccgctggcgg tcagctcgcc ggtcgacagc
     7501 gtctgggcgc atccgccgac gctgctcgcc aaccgcgagc gcctgccgga gctcgcacgc
     7561 ctgctcggca tgaccccctc ggaactcgaa gccaaggtcc tgtcgcgcga gaagaaggaa
     7621 ttcatgtacc tgcgccgcgc gctcgaaccc gatgctgccg cgcgcatcca ggtgcgggcg
     7681 attcccggcg tgttcctgca gcgcgaatac cggcgcttct atccgtcggc cgaggtgacc
     7741 tcgcacctgc tcggcttcac cgacatcgac gacgacggtc aggaaggcat cgagctggcg
     7801 tggaactcga cgctgcaggg cgtgcccggc gagaagcgcg tgatccgtga ccgctacggc
     7861 cacaccgtcg acgaagtcga gagcgtgcgc gagccgcagc ccggacgcac ggtgacgctg
     7921 tcgatcgacc ggcgcatcca gtacatcgcc taccgcgcgg tcaaggccgc gatgctcgaa
     7981 cacggcgctg ccgccgccac tgccgtcgtg ctcgacgtgc agaccggcga ggtgctggcg
     8041 atggtcaacc agcccggcac caacccgaac gatcgcggcg cgatccgcgg cgacctgctg
     8101 cgcaaccgcg cgctgaccga cgtttacgaa cccggctcgg tcatgaagcc gttcacggtg
     8161 gcgatgggcc tcgaaagcgg caggttccgg ccggacacgc cgatcagcac cagccccggc
     8221 cacatgagcg tcgggcgcta cacggtgcgc gacacgcaca actacggcct gatcgacgtg
     8281 acgcgcgtga tcgtcaagtc gagcaacgtc ggcgccacca agatcgcgct ggcgctgcca
     8341 gccgagcggc tgtgggacct gttccgcaag ctcggcttcg ggcgcaccag cggcatcggc
     8401 ttcccgggcg agcagggcgg gtggctcgcg cactactcgc gctggagcac gatcagccac
     8461 gccaaccaga gcttcggcta cggcatctcg atgacgccgc tgcagctcgc gcgggcctac
     8521 gcggcactcg cctccggcgg cgtgatccgg ccggtgtcgc tggtcaagct cgaccgggcg
     8581 ccggagggcg agcgcatttt gtccgaggcc acggtcagga agctgatgcc gatgatggaa
     8641 gccgtcgtca gccgcgacgg cacggcgatc caggcgagcg tcgccggcta ccgcgtcgcc
     8701 ggcaagaccg gcaccgtcca caagatcgtc ggccgcgggt acgcgaagaa caactactac
     8761 tcggtgttcg cgggcttcgc gccggtcagc aagccgcggc tggcgatggt cgtcatcgtc
     8821 gatgacccga agggcaagtc ctactacggc gggctggtcg ccgcgccggt gttcagcaag
     8881 gtcatggccg gagcgctgcg cgtcatgaac gtgccgccgg acaacatcgt cgatccgcgc
     8941 ctgacgccgg cgcggccggt gatggcaagc acgacggcac cggctgccgc gccggcgacg
     9001 gcgaaacggg agcgtccgtg atgctgccgc tgtccgtcct gctcgccgga ttcgcccgcg
     9061 acgtgcctga actgccggtc acggcgctcg cgctcgacag ccgtgccgtg accccggggg
     9121 cggtgttctg cgccgtgcgc ggccacagcg gccacggtct cgaccacgtg cagcaggcga
     9181 tcgcgcgcgg tgcgagtgcc gtgctgtggg agccgccggc cgcggcaccg ctgctgccgg
     9241 tgccggcgat cccgatcgaa cggctcggcc ggcaggtgtc acagctggcg agccggctgt
     9301 acggcatccc gtcgcacgcg ctggcggtgc tcggcgtcac cggcaccgat ggcaagacct
     9361 cgtgcacgca cttcctcgcg caggcgcttt ccgaacccgg acgcccggcg ggcctgctcg
     9421 gcacgctcgg ctacggcctc tggggcgggc cgttcgagaa cgcctcgcac acgacccccg
     9481 atgcgatcgg cgtgcagcag cgcctcgccg cctttgtcga ggccggcgtg cgtcacgcgg
     9541 ccatggaagt ctcctcgcac gcgctcgatc aggggcgggt cgaggccgtg cacta
//
LOCUS       DAOQJH010000008         9433 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_3855_length_9433_cov_4.044892, whole genome shotgun sequence.
ACCESSION   DAOQJH010000008 DAOQJH010000000
VERSION     DAOQJH010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 9433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9433
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3855_length_9433_cov_4.044892"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(219..1628)
                     /gene="glnG"
                     /locus_tag="PLQ18_00440"
     CDS             complement(219..1628)
                     /gene="glnG"
                     /locus_tag="PLQ18_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003453342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HNE00142.1"
                     /translation="MARKEHVWVIDDDHSIRWVLEKALSRETMAVSAFDSGNAALAAL
                     ERGSAPDAIVADIRMPGINGLELLARLREQVPDLPVIIMTAHSDLDSAVAAYQGGAFE
                     YLPKPFDVDEAVALVRRACDLRRQQAASADHGGPMPEIIGEAPAMQEVFRAIGRLSRS
                     NITVLINGESGTGKELVARALHRHSPRAERPFIALNMAAIPRDLLESELFGHERGSFT
                     GAQAARQGRFEQADGGTLFLDEIGDMPSELQTRLLRVLAEGEFYRVGGHSPIKVDVRV
                     IAATHQDLENRVRQGQFREDLFHRLNVIRIHLPPLRKRREDIPLLMAHFLKLAARELD
                     VEPKTLRSDVEGFLQQLDWPGNVRQLENLCRWLTVMASGREVHMEDLPSELKLTPIGA
                     SVGGEGGGDWRQALSDWAAATLIRGESEILNTALPEFERTLINVALKHSGGHRQDAAR
                     LLGWGRNTLTRKIKELNME"
     gene            complement(1642..2703)
                     /gene="glnL"
                     /locus_tag="PLQ18_00445"
     CDS             complement(1642..2703)
                     /gene="glnL"
                     /locus_tag="PLQ18_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010961763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HNE00143.1"
                     /translation="MTAVVRTDYPRRVTDNLTTGVVVLDRHLRTVYMNPAAEMLFALS
                     FRQALGAHFRDLAIGADNLEAGMRRCLDSGHPYTERELTLTLPGEHVITVDSSVTPVV
                     EHEQILELLVELRQVDRQLRISREENLIAQHTTTQVLVRNLAHEIKNPLGGLRGAAQL
                     LERELPDERLKEYTRIIIHEADRLRGLVDRMLGPMHPPARQAVNLHEVLERVRTLVVA
                     EGLGSTEIVRDYDPSIPPLQGDFDQLVQAVLNIVRNASQALAGTGTITLRTRVTRQCT
                     IGSRRYKLVARLDIVDDGPGIPPAIKERIFYPMITGRPDGTGLGLSIAQGLVSQHGGL
                     IECNSVPGDTTFTLLLPLE"
     gene            complement(3034..4443)
                     /gene="glnA"
                     /locus_tag="PLQ18_00450"
     CDS             complement(3034..4443)
                     /gene="glnA"
                     /locus_tag="PLQ18_00450"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010960935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HNE00144.1"
                     /translation="MSAEDVLSKIKDEGIKFVDFRFTDTRGKEQHVTVPAHTVDADVF
                     ESGKMFDGSSISGWKGINESDMILMPDPESAFVDPFFDEPTINITCDVIEPATMQGYE
                     RDPRSVARRAEAYLQSTGIADTAYFGPENEFFIFDDVRYSTEIGRVGYQIDSEEAAWN
                     TNKQYSDGNTGHRPGIKGGYFPVPPVDSLHDIRAAMCLALAEIGQRVEVHHHEVATAG
                     QCEIGVGFHTLVRKADEVQSLKYIIQNVAHNYGKTATFMPKPLHGDNGSGMHVHQSLA
                     KDGKNLFTGDLYGGLSEMALFYIGGIIKHAKALNAFTNASTNSYKRLVPHFEAPVMLA
                     YSARNRSASIRIPFVSNPKGRRIEVRFPDSTANPYLAFAAMMMAGLDGIQNKIHPGEA
                     MDKDLYDLPPEEEALIPQVCFSLDQALAELDKDREFLTKGGVFTDDLIDAYIGLKNQE
                     VQKVRMIPHPAELELYYSL"
     gene            4869..6053
                     /locus_tag="PLQ18_00455"
     CDS             4869..6053
                     /locus_tag="PLQ18_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002932365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNE00145.1"
                     /translation="MNAPHAAAPHRPRVGLLLTGGGARAAYQVGVLQAIAEWLPPGAP
                     TPFAVISGSSAGAINAALLASHAGAFAAGVDALSRLWGRLACSDVFRTDWRAALVSGL
                     LWTGSLAAGGLGRLTPRSLLDSRPLRALLGRHIDFAAVRTAIEDGTLHAFSLSAAGYN
                     SGQSITFFMGHAGLRPWQRTRRIGMPRPLTLDHLMASCAIPFVFPAVRIGYDWYGDGS
                     MRQIAPLSAPLHLGADRLLVIGVREETPDEPDRPILRLEYPSPGQIAGYILDTLFMDA
                     LSADIERARRINRTLALIPPERRAQLALRPIEIATVFPSRDVRLIAAHHEAHFPRSVR
                     YLLHSLGATRGRGRPLISYLLFEGGFCGELIALGYRDAQDARERLCTLLGLAHPATTL
                     RP"
     gene            6230..7159
                     /gene="glyQ"
                     /locus_tag="PLQ18_00460"
     CDS             6230..7159
                     /gene="glyQ"
                     /locus_tag="PLQ18_00460"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002690332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HNE00146.1"
                     /translation="MRDVSTFQGLILALQEYWAEQGCAVLQPYDMEVGAGTFHTATTL
                     RAVGPEPWAAAYVQPSRRPKDGRYGENPNRLQHYYQFQVVIKPSPDDIQDLYLGSLAR
                     LGIDPLVHDIRFVEDNWESPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLDCRPVTGE
                     ITYGLERIAMYLQNVESVYDLTWTDGPFGRISYGDVFHQNEVEQSTYNFEQADTAWLF
                     RQFDQCESESARLVALGLALPAYEQMLKASHTFNLLDARKAISVTERQRFILRVRTLA
                     RACAQAYHDARAALGFPMCRTPAAITATTGTGA"
     gene            7171..9252
                     /gene="glyS"
                     /locus_tag="PLQ18_00465"
     CDS             7171..9252
                     /gene="glyS"
                     /locus_tag="PLQ18_00465"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HNE00147.1"
                     /translation="METNDLLVEIGTEELPPKALPALSEAFEAGIRDGLAKAGLNFGA
                     VHRYAAPRRLAVLVHELPMQQPERRLERRGPAVSAAFGPDGTPTKATEGFARSCGVEV
                     AALTRVATDKGEWLAHVSTEPGAPLAQLLPGIVEAALAALPIPKRMRWGARTQEFVRP
                     AHWIVLLHGTQVVPGEILGLPTGRETRGHRFHHPAPIAIPKPAAYAPLLADQGRVLAD
                     FASRREAIRNQAVRAAAGLGGEAVIRPELLDEVTALVEWPVVLVGSFEPRFLAVPNEA
                     LISTMQDNQKYFPVVDAQGRMLPHFITIMNIESRDPAQVRAGNERVIRPRFSDAEFFW
                     NQDRKRRLDSHVESLKTVVFQQKLGTLHDKSGRVAQLAQTLAGVLGADAALAARAAQL
                     AKCDLMTNMVGEFPELQGTMGRYYAAHDGEAAELAQALEEQYRPRFAGDAIATSATGR
                     IVALADRLDTLVGIFAIGQPPSGAKDPFALRRAALGALRTIIEGGLEIDLHATLTGAA
                     AAFPAALAAPAAVDAVFDFMIERLRAFYTERAGEPVRPDEFDAVAACRPTRPLDFDRR
                     LRALAAFRRLPEAESLAAANKRIRNILRKVEGRLPEAVRVELLVEAPEQALHAALAGL
                     AAEVTPLMDAGRYAEALERLAGLRAPVDAFFDGVLVMAEDPAVRDNRLALLNELGALF
                     LRVADLSRLQG"
BASE COUNT         1394 a         3304 c         3245 g         1490 t
ORIGIN      
        1 ccgtcaccgc ggccggtgct cggcctgggc aagcgcccga agccggccgg gtagacgcac
       61 gccaccggca cggtgccgcg gcgcagcgtc cgaagtcgtc cgagggaatc cgaggccagc
      121 cggtgcgcaa acggtacatc tgacggtaca tctagccgga ttcgcgccgg caacaaagac
      181 aattgccatt gattttcaat gaaaataccc ggcaatgatc attccatgtt cagttccttg
      241 atcttgcgcg tcagcgtgtt gcggccccag ccgagcagcc gggcggcgtc ctggcggtgg
      301 ccgccggagt gcttcagtgc gacgttgatc agcgtgcgct cgaactccgg cagcgcggtg
      361 ttgaggatct cgctctcgcc gcggatcagc gtcgccgcgg cccagtccga cagcgcctga
      421 cgccagtcgc cgccgccctc gccgccgacg ctcgcgccga tcggcgtcag cttcagctcg
      481 ctcggcaggt cctccatgtg cacttcgcgg cccgaggcca tcaccgtcag ccagcggcac
      541 aggttttcga gctggcggac gttgccgggc cagtcgagct gctgcaggaa gccttcgaca
      601 tccgagcgca gcgtcttcgg ctccacgtcg agttcgcgcg ccgcgagctt caggaagtgc
      661 gccatcagca gcgggatgtc ctcgcggcgc ttgcgcagcg gcggcaggtg gatgcggatg
      721 acgttcagcc ggtggaacag gtcctcgcgg aactgcccct gacgcacccg gttctcgagg
      781 tcctgatggg tcgcggcgat cacgcgcacg tcgaccttga tcgggctgtg accgccgacg
      841 cggtagaact cgccctcggc gagcacgcgc agcagccgcg tctgcagctc cgagggcatg
      901 tcgccgatct cgtcgaggaa cagcgtgccg ccgtcggcct gctcgaagcg gccctgacgc
      961 gcggcctgcg cgccggtgaa ggagccgcgc tcgtggccga acagctcgga ttcgagcaga
     1021 tcgcgcggga tcgccgccat gttcagcgcg atgaacggcc gctcggcgcg cgggctgtgc
     1081 cggtgcagcg cgcgggcgac cagctccttg ccggtgccgg attcaccgtt gatcagcacc
     1141 gtgatgttcg agcgcgacag ccggccgatg gcgcggaaca cctcctgcat cgccggcgcc
     1201 tcgccgatga tctccggcat cggtccgccg tgatcggcgc tggccgcctg ctggcggcgc
     1261 aggtcgcagg cgcggcgcac cagcgcgacg gcttcatcga catcgaaggg cttgggcagg
     1321 tactcgaagg caccgccctg ataggcggcg acggcgctgt cgaggtcgga gtgcgcggtc
     1381 atgatgatga ccggcaggtc cggcacctgc tcgcgcagcc gcgccagcag ttcgaggccg
     1441 ttgatgccgg gcatgcggat gtcggcgacg atcgcatccg gcgcgctgcc gcgttcgagc
     1501 gcggcgagcg cggcgttgcc gctgtcgaag gccgaaaccg ccatcgtctc ccggctcagg
     1561 gccttttcca gcacccagcg gatcgaatgg tcgtcgtcga tgacccatac gtgttctttt
     1621 ctggccatgg cgggctctcg cttactccag cggcagcagc aacgtgaagg tggtgtcgcc
     1681 gggcacgctg ttgcactcga tcaggccgcc gtgctggctc accagtccct gggcgatgga
     1741 caggccgagc ccggtgccgt cggggcggcc ggtgatcatc gggtagaaga tgcgttcctt
     1801 gatcgccggc gggatgccgg gaccgtcgtc gacgatgtcg aggcgcgcga ccagcttgta
     1861 gcggcgcgag ccgatcgtgc actggcgcgt cacgcgcgtg cgcagcgtga tcgtgccggt
     1921 gccggcgagc gcctgcgagg cgttgcgcac gatgttgagc accgcctgca ccagctggtc
     1981 gaagtcaccc tgcagcggcg ggatgctcgg atcgtagtcg cgcacgatct cggtgctgcc
     2041 gagcccttcg gcgaccacca gcgtgcgcac gcgctccagc acctcgtgca ggttcacggc
     2101 ctggcgcgcc ggcgggtgca tcgggccgag catgcgatcg acgaggccgc gcaggcggtc
     2161 ggcctcgtgg atgatgatgc gggtgtattc cttcaggcgc tcgtccggca gctcgcgttc
     2221 gagcagttgc gcggccccgc gcaggccgcc gagcggattc ttgatctcgt gggcaaggtt
     2281 gcgcaccagc acctgcgtgg tcgtgtgctg cgcgatcagg ttctcctcgc gcgagatgcg
     2341 cagctgccgg tcgacctgac gcagctcgac cagcagttcg aggatctgct cgtgctcgac
     2401 caccggcgtg accgagctgt cgacggtgat cacgtgctcg ccgggcagcg tcagcgtcag
     2461 ctcgcgctcg gtgtacggat ggccgctgtc gaggcagcgg cgcatgccgg cttcgaggtt
     2521 gtcggccccg atcgccagat cgcggaaatg cgcgccgagc gcctgccgga aggacagcgc
     2581 gaacagcatc tcggccgccg gattcatgta gaccgtgcgc agatgacggt cgaggaccac
     2641 gacgccggtc gtcaggttgt ccgtcacgcg gcgcggataa tcggttctca ccacggctgt
     2701 catctccgga tacatgccgt cgggtttagc aagaagtggt ccaaccgggt gcaagcgccg
     2761 gaatcccgtc cggttttttg ggccggcgcg cagttgcagc gcgccagcgg cgggcagtgg
     2821 cgcacggcga tccggacgcg atgcaccatc atcaaccaca gcaccgcttt ggtgcgcatc
     2881 ctggcggtgc aaacctttgc tgcacgatcg tgcagccggg cggtccgcgc cacgatcgcg
     2941 cccgccagct gcgggcaaga aaaaaccccg tgccgcaggg cacggggttc cggcgccggg
     3001 cggccgcgtg cagcggccgt cagcggagcg atctcagagg ctgtagtaca gctcgagctc
     3061 ggccgggtgc gggatcatgc gcaccttctg gacttcctga ttcttcaggc cgatgtaggc
     3121 atcgatcagg tcgtcggtga acacgccgcc cttggtcagg aactcgcggt ccttgtccag
     3181 ctcggccagc gcctggtcga ggctgaagca gacctgcggg atcagggctt cctcttccgg
     3241 cggcaggtcg tacagatcct tgtccatggc ctcgcccgga tggatcttgt tctggatgcc
     3301 gtcgagaccg gccatcatca tcgccgcgaa cgccaagtag ggattggcgg tggaatccgg
     3361 gaagcgcacc tcgatgcggc ggcccttcgg attcgacacg aacgggatgc ggatcgacgc
     3421 cgagcggttg cgggccgagt aggccagcat gaccggggct tcgaagtgcg gcaccaggcg
     3481 cttgtagctg ttggtcgagg cgttcgtgaa cgcgttcagc gccttggcgt gcttgatgat
     3541 gccgccgatg tagaacagcg ccatttccga caggccgccg tacaggtcgc cggtgaacag
     3601 gttcttgccg tccttcgcca gcgactggtg cacgtgcatg cccgagccgt tgtcgccgtg
     3661 cagcggcttc ggcatgaagg tcgccgtctt gccgtaattg tgcgcgacgt tctggatgat
     3721 gtacttcagc gactgcactt cgtcagcctt gcgcaccagc gtgtggaagc cgacgccgat
     3781 ctcgcactgg ccggcggtcg cgacttcgtg gtggtgcact tcgacgcgct ggccgatctc
     3841 ggcgagcgcc aggcacatcg cggcgcggat gtcgtgcagg ctgtcgaccg gcggcaccgg
     3901 gaagtagccg cccttgatgc ccggacggtg accggtgttg ccgtcgctgt actgcttgtt
     3961 ggtgttccag gcggcttctt cggagtcgat ctggtagccg acgcggccga tctcggtcga
     4021 gtagcgcacg tcgtcgaaaa tgaagaattc gttctccggg ccgaagtacg cggtatcggc
     4081 gatgccggtg ctctgcaggt aggcctcggc gcggcgcgcc accgagcgcg gatcacgctc
     4141 gtagccctgc atcgtcgccg gttcgatgac gtcgcaggtg atgttgatcg tcggttcgtc
     4201 gaagaacgga tcgacaaagg cggattccgg atccggcatg aggatcatgt cggactcgtt
     4261 gatgcccttc cagccggaga tcgacgagcc gtcgaacatc ttgccgctct cgaacacgtc
     4321 cgcatcgacg gtgtgcgccg ggacggtgac gtgctgctcc ttgccgcgcg tgtccgtgaa
     4381 gcggaagtcc acgaacttga taccttcgtc tttgatcttg ctcagaacgt cttcggcaga
     4441 catctagttt cctccgcgat gagaacgggc tttgactcag cgattcaaaa acaacaaaaa
     4501 gcatgaaaac gaagttttcg gcgaggcatc ggttcagcac gatacatgcc actcgtgccg
     4561 aggcacccga tccggtcatc cggcaggggg ccatgcaccg ccacgaccgg ctgatgcacg
     4621 ataacagtgc aaaaagaagt gcatccgcac cggcagggag cggtattgac ggcttgttgc
     4681 gcttcacccc tcgcgcgcat cccgcggcgg cgtatgttgc cactgctgcc ggaaaaattg
     4741 catcccggca aggttcggac cccatctgtg gataagtttc tggataaacc ctccggttat
     4801 ccacatgggc atgtcgcagt gcagcgcgaa tgccggcagc acccggcacg gcgcggacgc
     4861 ggccacccat gaacgcaccg cacgcagccg caccgcaccg gccgcgggtc gggctgctgc
     4921 tgaccggcgg cggcgcacgc gcggcgtatc aggtcggcgt gctgcaggcg atcgccgaat
     4981 ggctgccgcc cggcgcgccg acgccgttcg cggtgatctc cggcagctcg gccggggcca
     5041 tcaacgccgc gctgctcgca agccacgccg gcgccttcgc ggccggtgtc gacgcactct
     5101 cccggctgtg gggcaggctg gcctgctccg atgtgttccg caccgactgg cgcgcagcgc
     5161 tcgtcagcgg actgctgtgg accggctcgc tcgccgccgg cggcctcggg cggctgacgc
     5221 cgcgctcgct gctcgacagc cggccgctgc gcgcgctgct cggccggcac atcgacttcg
     5281 cggcggtgcg cacggcgatc gaggacggca cgctgcacgc gttcagcctc agcgccgccg
     5341 gctacaactc cgggcagtcg atcaccttct tcatgggcca tgcggggctg cggccctggc
     5401 agcgcacgcg gcgcatcggc atgccgcggc cgctgacgct cgatcacctg atggcctcgt
     5461 gcgcgatccc gttcgtgttc ccggccgtgc gcatcggcta cgactggtac ggcgacggct
     5521 cgatgcgcca gatcgcgccg ctgtcggcgc cgctgcacct cggcgccgac cggctgctgg
     5581 tgatcggcgt gcgcgaggag accccggacg aacccgaccg gccgatcctg cgcctcgaat
     5641 acccgtcgcc gggccagatc gccggctaca tcctcgacac gctgttcatg gatgcgctgt
     5701 cggccgacat cgagcgcgcg cggcgcatca accgcacgct ggcgctgatc ccgcccgagc
     5761 gccgcgcgca gctggcgctg cggccgatcg agatcgccac cgtgttcccg agccgcgacg
     5821 tgcgcctgat cgccgcgcac cacgaagcgc acttcccgcg ctcggtgcgc tacctgctgc
     5881 acagcctcgg cgcgacgcgc ggacgcggcc ggccgctgat cagctatctg ctgttcgaag
     5941 gcggcttctg cggcgagctg atcgcgctcg gctaccgcga cgcacaggac gcgcgcgagc
     6001 gcctctgcac gctgctcggc ctcgcgcatc cggccaccac gctgcggccc tgaagcccgc
     6061 tgcacgacgg gccggccgtt cccacgcggg cggtttcacg tgaaaccgcc ccggcccgtg
     6121 cgcctgcgcc aacttcggac ggatgaacgg atatactcgc gcgtccccgg ccccgccccc
     6181 ggtgcgatca ccggcgcggg gggctgtcga gcgagcggag aaagccagcg tgcgcgatgt
     6241 ttcgacgttt cagggcctga tcctggccct gcaggaatac tgggccgagc agggctgtgc
     6301 ggtgctgcag ccctacgaca tggaagtggg cgccggcacc ttccacaccg cgaccacgct
     6361 gcgggcggtc ggaccggaac cgtgggcggc ggcctacgtg cagccctcgc gccgcccgaa
     6421 ggacggccgc tacggcgaga acccgaaccg gctgcagcac tattaccagt tccaggtcgt
     6481 catcaagccc tcgccggacg acatccagga cctctacctc ggctcgctcg cgcgcctcgg
     6541 catcgacccg ctggtgcacg acatccgctt cgtcgaggac aactgggaat cgccgacgct
     6601 cggcgcctgg ggcctcggct gggaggtctg gctcaacggc atggaagtca cgcagttcac
     6661 gtacttccag caggtcggcg gcctcgactg ccggccggtg accggcgaga tcacctacgg
     6721 tctcgaacgc atcgcgatgt atctgcagaa cgtcgagagc gtctacgacc tgacctggac
     6781 cgacggcccg ttcggacgca tcagctacgg cgacgtgttc caccagaacg aggtcgagca
     6841 gtcgacctac aacttcgagc aggccgacac cgcctggctg ttccggcagt tcgaccagtg
     6901 cgaatccgaa tcggcgcggc tggtcgcgct cgggctggcg ctgccggcct acgagcagat
     6961 gctcaaggcc tcgcacacct tcaatctgct cgatgcgcgc aaggcgatct cggtgaccga
     7021 gcgccagcgc ttcatcctgc gcgtgcgcac gctcgcacgc gcctgcgcgc aggcctatca
     7081 cgatgcccgc gccgcgctcg gcttcccgat gtgccggacc cccgcagcga tcacggccac
     7141 gaccggaacg ggagcctgaa catgtcgcag gtggagacga acgatctgct ggtcgagatc
     7201 ggcaccgagg agctgccgcc gaaggccctg ccggcgctct cggaagcctt cgaggccggc
     7261 atccgcgacg gcctcgccaa agccggcctg aacttcggcg ccgtgcaccg ctacgccgcg
     7321 ccgcgccgcc tcgccgtgct cgtgcatgag ctgccgatgc agcagcccga gcgccggctg
     7381 gagcgccgcg gtccggcggt gagcgccgcc ttcggcccgg acggcacgcc gacgaaggcc
     7441 accgaaggct tcgcgcgctc ctgcggtgtc gaggtcgcgg cgctgacgcg cgtggcgacc
     7501 gacaagggcg agtggctcgc gcacgtcagc accgagcccg gcgcgccgct cgcgcagctg
     7561 ctgcccggca tcgtcgaggc cgcgctcgcg gcgctgccga tccccaagcg catgcgctgg
     7621 ggcgcgcgca cgcaggaatt cgtgcgcccg gcgcactgga tcgtgctgct gcacggcacg
     7681 caggtcgtgc ccggcgagat cctcggcctg ccgaccgggc gcgagacgcg cggtcaccgc
     7741 ttccaccatc ccgcgccgat cgcgatcccg aagccggccg cgtatgcgcc gctgctcgcc
     7801 gatcagggcc gcgtgctcgc cgatttcgcc agccggcgcg aggcgatccg caaccaggcg
     7861 gtgcgcgcgg cggccggcct cggcggtgag gcggtgatcc gccccgagct gctcgacgag
     7921 gtcacggcgc tggtcgagtg gccggtcgtg ctggtcggca gcttcgagcc gcgcttcctc
     7981 gcggtgccga acgaggcgct gatctcgacg atgcaggaca accagaagta cttcccggtc
     8041 gtcgatgcgc aggggcgcat gctcccgcac ttcatcacga tcatgaacat cgagtcgcgc
     8101 gacccggcgc aggtgcgcgc cggcaacgag cgcgtgatcc ggccgcgctt cagcgacgcc
     8161 gagttcttct ggaaccagga ccgcaaacgc cgcctcgaca gccacgtcga gagcctcaag
     8221 accgtcgtct tccagcagaa gctcggcacg ctgcacgaca agtccggacg cgtcgcgcag
     8281 ctggcgcaga cgctcgccgg cgtgctcggc gccgatgccg cgctcgccgc ccgcgccgcg
     8341 cagctcgcca agtgcgacct gatgacgaac atggtcggcg agttcccgga gctgcagggc
     8401 acgatgggcc gctactacgc cgcccacgac ggcgaagcgg ccgaactcgc gcaggccctg
     8461 gaggaacagt accggccgcg cttcgccggc gacgccatcg ccaccagcgc caccggccgc
     8521 atcgtcgcgc ttgccgatcg cctcgacacg ctggtcggca tcttcgcgat cggccagccg
     8581 ccctcgggcg cgaaggaccc gttcgcgctg cgccgcgccg cgctcggggc gctgcgcacg
     8641 atcatcgagg gcgggctgga aatcgacctg cacgcgacgc tgaccggggc cgccgcggcc
     8701 ttcccggcgg cgctggcagc cccggcggcc gtcgatgcgg tgttcgactt catgatcgag
     8761 cggctgcgcg cgttctacac cgagcgcgcc ggcgagccgg tgcggcccga cgagttcgat
     8821 gccgtcgccg cctgccgccc gacccggccg ctcgacttcg accgccggct gcgcgcgctc
     8881 gcggccttcc gccgcctgcc cgaggccgaa agcctcgccg ccgccaacaa gcgcatccgc
     8941 aacatcctgc gcaaggtcga gggccggctg ccggaagcgg tgcgcgtcga gctgctggtc
     9001 gaggccccgg agcaggccct gcacgccgcg ctcgccggac tggccgccga ggtcacgccg
     9061 ctgatggacg ccgggcgcta cgccgaggcg ctcgaacgcc tggccgggct gcgggcgccg
     9121 gtcgatgcgt tcttcgacgg cgtgctggtg atggccgaag acccggcggt gcgcgacaac
     9181 cgcctcgcgc tgctgaacga gcttggtgcc ctgttcctgc gcgtcgccga cctgtcgcgc
     9241 ctgcagggct gagcccgcat cgcgccgccg gagctgacgc cggtcaacgg caggctccgg
     9301 cggcgcgcgc agcatcatcg gtcgccgccg gcggccagca tgacccgtga cggcccctgc
     9361 ccgggctgat ccgtgatacc gatccgcgtc ccgaagccgg cgcggcgttc ctgagtcgat
     9421 ctgattgtct gcg
//
LOCUS       DAOQJH010000009         9180 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_4012_length_9180_cov_3.932274, whole genome shotgun sequence.
ACCESSION   DAOQJH010000009 DAOQJH010000000
VERSION     DAOQJH010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 9180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9180
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4012_length_9180_cov_3.932274"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(212..1324)
                     /locus_tag="PLQ18_00470"
     CDS             complement(212..1324)
                     /locus_tag="PLQ18_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011395059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HNE00148.1"
                     /translation="MFSPRRRQLLQFAAATPLAAAFASVPRPARAAFPKPELPLKVGY
                     VYVSPIGDAGWTYQHDLGRKAVEASFGDKVMIKVIESVSEGADAERVIREFASSGYGL
                     VFATSFGYMNPLLRVAKQFPKGIFEHATGYRQAPNVGIYAARYYEARYLTGILAGRMS
                     KSGVAGYVAAFPIPEVVMGINAFMLGMKSVNPQAQVKVIWVNAWFDPGKEREAAATLV
                     AQGADVLTQHTDSTAVVQTAEDKGAYAIGYHSDMSKYGPKAHLSAATHHWEGYYRRVV
                     QQVLDGSWAPDNVWGGIKDGMVGLAPLHPAVPAEVGALVATKKQDIIDGRLLPFQGPL
                     FDQAGRQVIGAGAKLTDDELLKMNYYVEGVVGQMPK"
     gene            complement(1504..2400)
                     /locus_tag="PLQ18_00475"
     CDS             complement(1504..2400)
                     /locus_tag="PLQ18_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012974675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNE00149.1"
                     /translation="MSLSGWLPILAGVLAAATPLLIAALGELIAEKSGVLNLGVEGMM
                     LVGALTAFAVASGGAGYAAGALAGALAGVGLAALFAWLTLNCLASQVACGLAITMLGS
                     GLSAYAGRDYVSRTLDGMALIPLGGGFGFDPLIVTGLLLTVLVWYFLQRSRAGLVLRA
                     VGESPATAHALGLPVRRIRWAAVLFGGACAGLAGAYLAIDYTPMWVEGLSAGRGWIAL
                     ALVVFAGWRPGRTLLGAWLYGGVGILQFHAQALGIAVPAQFLGMLPYLATIVVLTLIA
                     RDPLTQRRHTPASLGQPWHPHA"
     gene            complement(2397..3485)
                     /locus_tag="PLQ18_00480"
     CDS             complement(2397..3485)
                     /locus_tag="PLQ18_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004264735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNE00150.1"
                     /translation="MFTLEARTLPSVRAMWLAPLAALLLTLLGGSVLFAAFGHAPLQA
                     LSVYFLQPLASVYGIGEWLLKATPLALCGLGLALGFRAKVWNIGADGQFTLGAICAGG
                     LAIACDGFIDAESLWIAPAMALAGIAGGMMWAALPAWLRTRYGSSEILSSLMLAYVAP
                     LLLGWLVNGPWRDPAGFNFPQSIQFADAALLAPLHEGARVTLAFPVALVLAGLLCFVQ
                     RYTYAGFRLQVSGEAPLAARHAGYSQRAAVWIAMLTGGACAGFAGYAEVAGPLGQLQP
                     SVSPGYGFTAIIVAFVGRLDPRGVVLAALLLSLLALGGEAAQMTLDLPAAISGVFQGG
                     LLCALLACDTFVHYRLRRVYRKPAARTS"
     gene            complement(3472..5025)
                     /locus_tag="PLQ18_00485"
     CDS             complement(3472..5025)
                     /locus_tag="PLQ18_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004359030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNE00151.1"
                     /translation="MSATIPRLELRAIRKHYSGTIANDDIDLVVHPGEIHAVLGENGA
                     GKSTLMKIIYGVVAPDAGELFWDGQPVRIHSPAQARRLGIGMVFQHFSLFESLSVAEN
                     IALALPDAPRPAALAGRIRRVSADYGLALEPMQPVHELSVGERQRVELVRCLLQSPRL
                     LILDEPTSVLTPQAVEALFVTLRRLAREGVSLLYISHKLEEIRRLCDQATVLRNGRVR
                     AHCRPAEESAASLARLMIGGELPATRLTPNALPRTPRLVLDRLSRTADGPFGTPLREL
                     ELCVHSGEIVGIAGASGNGQKELLEALFGEVALADAAMLQLAGEGIGRLGPAARRARG
                     LGMVPEERLGRAAVPARSLADNTLLTAAGHGLVRAGLIRRPAVLRFARALIEGFQVRC
                     KGAQAPANSLSGGNLQKFIVGRELAQHPQALVIAQPTWGVDVASAALIHEQLLRLRND
                     GVALLVISEDLDELFGLCDRIAVLAGGRLSPVRETGATDAAELGQWMGGGFGPVTAAA
                     APGMRDVHA"
     gene            complement(5286..5528)
                     /locus_tag="PLQ18_00490"
     CDS             complement(5286..5528)
                     /locus_tag="PLQ18_00490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE00152.1"
                     /translation="MTHASGWMSDESRVMTDDLRVLADDSRVMMDEAPVMTGELGFMS
                     DELQLMSDESRFMSDESRVMIHEFRVRTDESRFMPD"
     gene            5932..6297
                     /locus_tag="PLQ18_00495"
     CDS             5932..6297
                     /locus_tag="PLQ18_00495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE00153.1"
                     /translation="MANIRSKDFLSDMERKINSIRQFASDRVFGGVTLADLESARTAS
                     LNARTDIQHAENAMIALRNHRQESDRHYEGLMRRAVAGLIADPDFGPDSDAYDAMGYT
                     RYKERKRRTTKPRREPEDS"
     gene            6500..6637
                     /locus_tag="PLQ18_00500"
     CDS             6500..6637
                     /locus_tag="PLQ18_00500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE00154.1"
                     /translation="MNDPVPVTAEGDDEDQIDGCDVDCDGEATEYTPDEELPASDGGV
                     A"
     gene            6639..7439
                     /locus_tag="PLQ18_00505"
     CDS             6639..7439
                     /locus_tag="PLQ18_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HNE00155.1"
                     /translation="MAWSLIWLPEILKRAGLKVALVDGWENRGRGDMGRVEGVICHHT
                     VGSPNLNMPSLKVLINGRADLPGPLAQLGLGRDGTYYVIAAGRCNHAGKGSWNGQTHG
                     NSNFIGIEAENTGKADDLPWPEVQMDAYRRGVAAILKHVGKGADGCAGHKEYALPKGR
                     KPDPSFDMPAFRNEVAAILNGSAPAPVLIPAFEPATASTNSGAARPTLRRGASGELVK
                     HLQQKVGASVDGDFGPKTEAAVRVFQRQKGMVPDGIVGPKTWAAMDAV"
     gene            complement(7540..8061)
                     /locus_tag="PLQ18_00510"
     CDS             complement(7540..8061)
                     /locus_tag="PLQ18_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004271320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2199 domain-containing protein"
                     /protein_id="HNE00156.1"
                     /translation="MSFSFQCSICGQVHEGMPSFGAAAPLNYYQIPVEEREARCALGS
                     DDCVIDGNFFFVRGCIEIPVHGQDEAFNWGVWVSLSQASYLNWLKYFNEPRRAHIGPF
                     FGWLNAWLHPYPDTVNLKTQIHLRDDGIRPYIELEPTDHPLAIEQRTGISTERVAEIY
                     ALIVHEDDALSAG"
     gene            complement(8142..8582)
                     /locus_tag="PLQ18_00515"
     CDS             complement(8142..8582)
                     /locus_tag="PLQ18_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007919355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNE00157.1"
                     /translation="MQWSKTDYIVSTNPEDQDLDVIHGFLSQSYWARNIPRETVARSL
                     QGALCFALLHKGRQIGFARVISDSATIAYLGDVFVLPEYRGRGLAGWLLECVTAHPQL
                     QGLRRWILATADAHGLYEKFGFTALKRPEIFMELHDPEVYSADR"
     gene            8787..>9180
                     /locus_tag="PLQ18_00520"
     CDS             8787..>9180
                     /locus_tag="PLQ18_00520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3 family protein"
                     /protein_id="HNE00158.1"
                     /translation="MMTAAIPAIDDRPPLPGDLAMWFFILAELTVFAALFLAFAYARL
                     WHTAEFAAGQTLIDRGAGAINTVLLITSSAFVVQAVACIRADDRPGCGRWLLAAIATG
                     LGFVVFKSAGFAQAFAAGVSLDGGVFPMF"
BASE COUNT         1667 a         3018 c         3023 g         1472 t
ORIGIN      
        1 cggcatgtcc gcgatacagt caggccggca tgagtcattt caaaatgaca aatcccgatt
       61 gaaagccttg tcgctggcct gcgatagtgc gggggcgggg cgttcccacg ggggctctcg
      121 ccagggctgc cgcccgcgct ggtcgggggg ccggcgcggg cggcgctctg tctgatccgg
      181 atgcagacag ccgccgccgc tgccggtcac ttcacttcgg catctgcccg accacgccct
      241 cgacgtagta attcatcttc agaagctcgt catcggtgag cttcgcgccg gcaccgatga
      301 cctgccggcc ggcctgatcg aacagcggcc cctggaaggg cagcagcctg ccatcgatga
      361 tgtcctgctt cttcgtcgcc accagcgccc cgacctcggc cggcaccgcc ggatgcagcg
      421 gtgccagtcc gaccatgccg tccttgatgc cgccccagac attgtccggc gcccagctgc
      481 cgtcgagcac ctgctgcacg acccggcggt aatagccctc ccagtgatgg gtcgccgcgc
      541 tcagatgcgc cttcgggcca tatttgctca tgtccgagtg atagccgatc gcgtacgcgc
      601 ccttgtcctc ggcggtctgc acgacggcgg tcgaatcggt gtgctgcgtc agcacgtcgg
      661 cgccctgcgc gaccagcgtc gcggccgcct cgcgctcctt gcccggatcg aaccaggcgt
      721 tcacccagat caccttgacc tgcgcctgcg ggttgacgct cttcatgccg agcatgaagg
      781 cgttgatgcc catcaccacc tccgggatcg ggaaggcggc gacgtagccg gcgacgccgg
      841 acttcgacat gcggccggcg aggatgccgg tcagatagcg cgcctcgtag tagcgcgccg
      901 catagatgcc gacgttcggt gcctgccggt agccggtcgc gtgctcgaag atgcccttcg
      961 gaaactgctt cgcgacgcgc agcagcgggt tcatgtagcc gaacgaggtc gcgaacacga
     1021 gcccgtagcc gctgctcgcg aactcgcgga tcacgcgctc ggcatcggca ccctcgctga
     1081 cgctctcgat gaccttgatc atcaccttgt cgccgaagct cgcctcgacc gccttgcggc
     1141 cgagatcgtg ctgataggtc cagccggcgt cgccgatcgg actgacgtag acgtagccga
     1201 ccttcagcgg cagttcgggc ttcgggaagg ccgcccgggc cgggcgcggc acactggcga
     1261 aggcggcggc gagcggggtg gcggcggcaa actgcagcaa ctggcgacgg cggggactga
     1321 acatgcttcg gaaccggttc cttgggacat ggggccttgc ggacgatggg gccggaggac
     1381 gcgggcgggg ttgcgctccg gcaccggacg ggctcttccg gggtttgcgg ctttgcagca
     1441 tggtgatttt atcgccggat gcgggcgctg cctgcgcaag catgctgacg cggcgggccg
     1501 gcttcaggca tgcggatgcc agggctgtcc gagactcgcc ggcgtgtggc gacgctgcgt
     1561 gagcgggtcg cgcgcgatca gcgtcagcac gacgatggtc gccagatacg gcagcatgcc
     1621 gaggaactgc gccggcacgg cgatgccgag cgcctgcgca tggaactgca ggatgccgac
     1681 gccgccgtac agccacgccc cgagcagcgt gcgccccgga cgccagccgg cgaacaccac
     1741 cagcgcgagc gcgatccagc cacggccggc gctgagcccc tcgacccaca tcggcgtgta
     1801 gtcgatcgcg agatacgccc cggccagccc cgcgcaggcc ccgccgaaca gcacggccgc
     1861 ccagcggatg cggcgcaccg gcagcccgag cgcatgcgca gtcgccggcg actcgccgac
     1921 ggcgcgcagc accagtccgg cgcggctgcg ctgcaggaag taccagacca gcaccgtcag
     1981 cagcagaccc gtgacgatca gcggatcgaa gccgaagccg ccgcccagcg ggatcagcgc
     2041 catgccatcg agcgtgcggc tgacgtagtc gcgcccggcg taggccgaca gcccgctgcc
     2101 gagcatcgtg atcgccagtc cgcaggcaac ctggctggcg aggcagttca gcgtcagcca
     2161 cgcgaacagc gccgcgagtc cgacgccggc aagcgccccg gcgagcgccc cggccgcgta
     2221 gccggcgccg ccgctcgcaa ccgcgaaggc cgtcagcgcc ccgaccagca tcatgccctc
     2281 gacgccgagg ttcaggacgc cgctcttctc ggcgatcagc tcgccgagcg cggcgatcag
     2341 cagcggtgtg gccgctgcca gcacgccggc aaggatcggc agccagcccg acaggctcat
     2401 gaggtgcgcg ccgcaggctt gcggtacacg cgccgcagcc ggtaatgcac gaaggtgtcg
     2461 caggcgagca gcgcgcacag cagtccgccc tggaacacgc cgctgatcgc cgccggcaga
     2521 tcgagcgtca tctgcgccgc ctcgccgccg agcgcaagca gcgacagcag cagcgccgcc
     2581 agcaccacgc cgcgcggatc gagccggccg acgaaggcga cgatgatcgc cgtgaagcca
     2641 tatccgggcg acaccgacgg ctgcagctgc ccgagcgggc cggcaacctc ggcgtaaccg
     2701 gcaaaaccgg cgcaggcccc gcccgtgagc atcgcgatcc acaccgcggc gcgctgcgaa
     2761 tagccggcat gacgcgccgc gagcggtgcc tcgccgctga cctgcagccg gaagccggcg
     2821 taggtgtaac gctgcacgaa gcagagcagc ccggcgagga cgagcgccac cggaaacgcc
     2881 agcgtcacgc gcgcgccttc gtgcagcggc gccagcagcg cggcatccgc gaactggatc
     2941 gactgcggaa agttgaaacc ggccggatcg cgccacggac cattgaccag ccagccgagc
     3001 agcagcggcg cgacgtaggc cagcatcagg ctgctcagga tttcgctgct gccgtagcgc
     3061 gtgcgcagcc aggccggcag cgccgcccac atcatgccgc cggcaatgcc ggcgagcgcc
     3121 atcgccggcg cgatccagag actttcagcg tcgatgaagc cgtcgcaggc gatcgcgagt
     3181 ccgccggcgc agatcgcccc gagcgtgaac tggccgtcgg caccgatgtt ccagaccttg
     3241 gcccggaagc cgagcgcgag cccgagcccg cagagcgcga gcggtgttgc cttcagcagc
     3301 cattcgccga tgccgtagac actggcgagc ggctgcagga agtacacgga cagcgcctgc
     3361 agcggtgcat gaccgaaggc tgcgaacaac acgctgccgc cgagcagggt cagcagcagc
     3421 gccgcgagcg gcgcgagcca catcgcccgc accgaaggca gcgtccgcgc ttcaagcgtg
     3481 aacatcccgc atccccggtg ctgctgctgc cgtcaccggc ccgaaaccgc cccccatcca
     3541 ctgcccgagt tcggcggcat cggtggcccc ggtctcccgg accggcgaca gccgtccgcc
     3601 ggcgagcacg gcgatgcgat cacagaggcc gaacagctcg tcgaggtcct cggagatcac
     3661 cagcagcgcc acgccgtcgt tgcgaagccg cagcagctgt tcgtggatca gcgccgccga
     3721 ggcgacatcg acgccccagg tcggctgcgc gatcaccagc gcctgcggat gctgcgccag
     3781 ctcgcgcccg acgatgaact tctgcaggtt gccgcccgaa agactgttcg ccggcgcctg
     3841 cgcgcccttg cagcgcacct gaaagccttc gatcagcgcg cgcgcgaagc gcagcaccgc
     3901 cggccggcgg atcagcccgg cacgcaccag cccgtgaccg gcggcggtga gcagcgtgtt
     3961 gtccgcgagc gagcgcgccg gcaccgccgc gcggccgagc cgctcctccg gcaccatgcc
     4021 gaggccgcgc gcgcgccgcg ccgccggtcc gagccggccg atgccctcgc cggcgagctg
     4081 cagcatcgct gcatcggcga gcgccacctc gccgaacagc gcttcgagca attccttctg
     4141 gccgttgccg gatgccccgg cgatgccgac gatctcgccg ctgtgcacgc aaagctccag
     4201 ctcccgcagc ggcgtgccga aaggcccgtc ggccgttcgc gacaagcgat cgagcaccag
     4261 ccgcggcgtg cgcgggagcg cattcggcgt cagccgcgtc gccggcagtt cgccgccgat
     4321 catcaggcgc gcgaggctcg cggccgattc ttcggccggg cggcaatgcg cgcggacgcg
     4381 cccgttgcgc agcaccgtgg cctggtcaca caggcggcgg atctcttcga gcttgtggct
     4441 gatgtagagc aggctcacgc cctcgcgcgc cagccgccgc agcgtcacga acagcgcctc
     4501 gaccgcctgc ggcgtcagca ccgaggtcgg ctcatcgagg atcagcaggc gtggcgactg
     4561 cagcaggcag cgcaccagct cgacgcgctg gcgctcgccg accgacagct cgtgcaccgg
     4621 ctgcatcggt tcgagcgcga ggccgtagtc ggccgaaacc cggcggatgc gtccggccag
     4681 cgccgccggg cgtggcgcat ccggcagggc cagcgcgatg ttttcggcaa cgctcagcga
     4741 ctcgaacagc gagaagtgct ggaacaccat gccgatgccg agccgccgtg cctgcgccgg
     4801 cgaatggatg cgcaccggct gcccgtccca gaacagctcg ccggcatcgg gcgccacgac
     4861 gccgtagatg atcttcatca gcgtgctctt gccggcgcca ttctcgccga gcacggcgtg
     4921 aatctcgccc ggatgcacga cgaggtcgat gtcatcgttg gcgatcgtgc cggaatagtg
     4981 cttgcggatc gcgcgcagtt cgaggcgggg gatggtggcg ctcatgcggt cggcgggggg
     5041 cgggcgaagg gcgcggacgg gcgcgaccgg gcgatggtag acgcggccgt gcgctcgcga
     5101 cagggcaatg tgctgcgctg catcagcaag gaaggcgtca tcggcaggcg ttgccggacg
     5161 acgcgaaggc cggaggcgag cggtggcagg gggccggcgc cgccgcacgc ggacgccggc
     5221 aacggtcgca gcaggcggac agccgcgatg ccgataaatc gcgcgtgcgc agcgatgaca
     5281 cacattcaat ccggcatgaa tcgtgattca tccgtcctga cacggaattc atgaatcatg
     5341 acacgcgact catcgctcat gaatcgtgat tcatcgctca tgagctgcag ttcatcgctc
     5401 atgaatccca attcaccggt catgaccggg gcttcatcca tcatgacgcg tgagtcatcc
     5461 gccaggacac gcaagtcatc ggtcatgaca cgtgattcat cgctcatcca tcccgatgca
     5521 tgggtcatgc tgcagctcag gccagagcct tgcgcagttc ggtcgctcgt tccggagata
     5581 aagagaactg gggagcaagg ggaaaaccac gcatctgccc ctgatcggca tgccatgcga
     5641 ttccggcatc acgccgggcg agtatcgaca ggagcggcgg atgtccggcg actccggagc
     5701 ggagtgtgtg cggcgcagac cacgtctgca agtccggaca tggcatggca gaagcataat
     5761 gtcggcgccg gccggatgga tggcgattat ttaatgtccg ctgaacttgt tgtggagcac
     5821 ttgttgatct agatgcttgg ttgcgtgcga cagcagtggc agccacagcc acgatcgtgc
     5881 atgccgcatt gcatcacgaa tcggcctgat ggaacgacaa ggagaaactg catggccaat
     5941 atccgttcga aggatttcct gtccgacatg gagcgcaaga tcaactccat ccgccagttt
     6001 gccagcgatc gcgtgttcgg cggcgtcacg ctggctgatc tggaaagcgc ccgtacggcc
     6061 agcctgaatg cgcgtaccga catccagcat gctgaaaacg ccatgatcgc gctgcgcaat
     6121 catcgccagg aatccgatcg ccattacgaa ggactgatgc gtcgcgccgt cgccggcctg
     6181 atcgccgatc ccgatttcgg tcccgatagc gatgcctatg acgcaatggg ctacacgcgc
     6241 tacaaggaac gcaagcggcg gacgacaaaa cccagacgcg aaccggaaga ttcctgagcc
     6301 ggtgccggac atggcctgcg gtgcctgcca tcgcaggcca tgcaatccgg atgaatctga
     6361 ccctcgcatc gggaatcggg cttgtctgca ccgcagtgac gactcgccat gaccatcggc
     6421 atcgatgtgt gattggcgat ttcccgatcc atcgatcatc cggacgtatc tcaacatctg
     6481 cagaacggag tgaaatgcca tgaatgatcc ggtaccggtc accgccgaag gtgacgatga
     6541 ggatcagatc gacggctgcg atgtcgattg cgacggcgaa gcgacggaat acaccccgga
     6601 cgaggaactg cctgccagcg atggcggcgt tgcctgacat ggcctggtcc ctgatctggt
     6661 tacccgagat cctgaaacgc gccggattga aggtggcgct ggtggatggc tgggaaaatc
     6721 gcggtcgcgg cgacatgggg cgtgtcgaag gcgtgatctg tcatcacacg gtcggttctc
     6781 cgaacctgaa catgccgagc ctgaaagtac tgatcaatgg ccgtgcggat ctgcccggtc
     6841 cgctggcaca gctcgggctc gggcgtgacg gcacgtatta cgtgatcgct gcgggtcgct
     6901 gcaaccatgc cggcaaaggt agctggaacg gccagacgca cggtaacagc aatttcatcg
     6961 gcatcgaggc cgagaacacc ggcaaggcgg acgatctgcc gtggccggag gtacagatgg
     7021 atgcctaccg ccgtggtgtg gcggcgatcc tgaagcatgt cggcaagggc gccgacggct
     7081 gcgccggtca caaggaatat gcactgccga agggacgcaa gcccgatccg agtttcgaca
     7141 tgccggcctt ccgcaacgag gttgccgcca ttctgaacgg cagcgcaccg gctccggtgc
     7201 tgattccagc cttcgaacct gcaactgcca gcacaaactc cggggctgcc cgcccgactt
     7261 tgcgccgggg tgccagtggt gaactggtga aacatctgca gcagaaggtc ggtgccagcg
     7321 tcgatggtga tttcggtccg aaaaccgaag ctgccgtgcg ggtgttccag cgccagaaag
     7381 gcatggtgcc ggatggcatc gtcggtccga agacctgggc ggcgatggat gctgtctgag
     7441 ttgcgggcga ctgcggtgaa cgtgatcagg tgaaagcttg gagcgcgcag tttgagggtt
     7501 cagtatgttc accccaacct gcgcgcccgc cggtcgttct cacccggcag ataatgcatc
     7561 gtcttcgtga acgatgagcg cataaatctc ggccacccgt tcagtggaaa tgccggttct
     7621 ttgctcgatc gctaaaggat gatcggttgg ctcaagttca atgtagggac ggatgccatc
     7681 gtcacgcagg tgaatctgcg ttttcaggtt tacggtatca gggtatggat gtaaccatgc
     7741 attgagccat ccgaagaatg gaccgatgtg agcgcgtcgg ggttcgttga aatacttcag
     7801 ccagttgagg tagctggcct gactgaggga gacccagact ccccagttga aagcttcatc
     7861 ctggccgtga accgggattt caatacagcc tcgtacaaaa aagaagttgc cgtcgattac
     7921 gcaatcatca ctgcctaaag cacaacgtgc ttcccgctct tcgacaggaa tctggtagta
     7981 gttcagtggg gcggcggcac caaaactcgg catgccttcg tgaacttgtc cgcaaatgct
     8041 gcattgaaaa ctgaaagaca tcgtcgatgg cctggtttgt attgagcgct gcattgactg
     8101 atcattcagg catgggactg catgaccgtt acgaggacgg atcaacgatc tgcggaataa
     8161 acttccgggt cgtgcagctc catgaatatc tccggccgtt tcagggcggt gaagccgaat
     8221 ttctcataca gcccatgcgc atccgcggtc gccagaatcc agcgccgcag gccctgcagc
     8281 tgcgggtgag ccgtaacgca ctccagcaac catccggcca gtccccgtcc ccgatactcg
     8341 ggcagcacga agacatcgcc aagataagcg atcgttgccg aatcactgat cacacgggca
     8401 aagccgatct gccggccctt gtgcagcagg gcgaagcaca gtgcgccctg aagtgatctt
     8461 gccacggttt ccctggggat gtttctggcc caataggatt ggctgaggaa gccgtggatg
     8521 acatcgagat cctgatcttc aggattcgtg ctgacgatgt aatctgtttt ggaccattgc
     8581 attgcggatg atctgctggt gtcgaggcag gtcgatgata acgtgaatcc ggatgagcat
     8641 ccggacccgt cctggctgtc gttcaggatc ccggttgcat caaaaccctg cataaacaag
     8701 gtcttgattc tcatcaaggc atccccggag cccatcctcc agtatcccga gcatcgccat
     8761 cggatatcgg aacaggagag ggccggatga tgactgccgc catacccgcc atcgacgatc
     8821 gtccgccgct gcccggcgat ctggcgatgt ggttcttcat cctcgccgag ctgacggtgt
     8881 tcgcggcgct gttcctggcc ttcgcgtatg cgcggctctg gcacacggcc gagttcgcgg
     8941 ccgggcagac gctgatcgat cgcggcgccg gagcgatcaa taccgtgctg ctgatcacgt
     9001 cgagtgcctt cgtcgtgcag gcggttgcat gcatccgtgc ggatgaccgt ccgggttgcg
     9061 ggcgctggct gctggcggcg atcgcgaccg ggctcggctt cgtcgtattc aagtccgcag
     9121 gcttcgcgca ggccttcgcg gccggggttt cgctcgatgg cggcgtgttt ccgatgttct
//
LOCUS       DAOQJH010000010         8906 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_4194_length_8906_cov_4.600271, whole genome shotgun sequence.
ACCESSION   DAOQJH010000010 DAOQJH010000000
VERSION     DAOQJH010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 8906)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8906)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8906
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4194_length_8906_cov_4.600271"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..343
                     /locus_tag="PLQ18_00525"
     CDS             <1..343
                     /locus_tag="PLQ18_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HNE00159.1"
                     /translation="AIEIGAGARVGANSVVIRDVPAAATVVGIPGRVVPRGDTARADG
                     IDLDHHLMPDPVGRALACLIQRIDDLEARLAAAGPAIPGLDHPHACTDCGDACDQARH
                     AAAAHPAALHS"
     gene            355..687
                     /locus_tag="PLQ18_00530"
     CDS             355..687
                     /locus_tag="PLQ18_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009564607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase-stabilizing/protective protein NifW"
                     /protein_id="HNE00160.1"
                     /translation="MSTLSDRLQTLSAADEFFELFKIDVDPHVVHVNRLHILKRFHDY
                     LKRSPVQADLSEADEFAAYRERLVQAYLDFTVSDAVTEKVFKVFRQAQGQGFVGLDQI
                     ERIERPAT"
     gene            737..1582
                     /locus_tag="PLQ18_00535"
     CDS             737..1582
                     /locus_tag="PLQ18_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HNE00161.1"
                     /translation="MHIVVCIKQVPDSAQIRVHPVTNTIMRQGVPAIINPYDLFSLEE
                     ALRLKDQFGGKVTVITMGPPQAEDALRKALSFGADEAILLTDRAFAGADTLATSCALA
                     QALRQIGEKEPVDLVFTGKQTIDGDTAQVGPGVAKRLDLQLLTYVAKIDELDLAGRTI
                     TVQRRAEGGVQVLKTRLPALITMLEGTNEMRFATMTDMFRAARSPLTVWNKDDAGITD
                     ISKIGLKGSPTVVSKVFAPKPKSVPAQMLDIGGKSQTEIADLIIDTLSAKHAPLYGEL
                     FDRAL"
     gene            1610..2704
                     /locus_tag="PLQ18_00540"
     CDS             1610..2704
                     /locus_tag="PLQ18_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011389921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HNE00162.1"
                     /translation="MSEAPKPAAPAARGRKKLELDPRLAAYKGVWVFIEHERGTVHPV
                     SWELLGEGRKLADKLGVELAGVLLGGPGDPLDAFAATAHTYGADLVYITRSPVLAGYR
                     NEPYTKALTAVVNQFQPEILLLGATVQGRDLAGSVATTLATGLTADCTELAIDPDNRS
                     LAATRPTFGGSLLCTIQTLNYRPQMATVRPRVMSMPAQQPERSGRVLEAPVELVEDDI
                     IAKLLSYIPDDATDQAQLAYADVIVAGGRGLGKPENFKLLWDLAEVLGAEVGGSRPVI
                     QNGILSADRQVGQTGKTVRPKLYIAAGISGAIQHRVGMEASDVIIAINTDPKAPIFDF
                     ATYGIVGDAVQILPALTAACRARLAMKRAG"
     gene            2716..4011
                     /locus_tag="PLQ18_00545"
     CDS             2716..4011
                     /locus_tag="PLQ18_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HNE00163.1"
                     /translation="MEKFDVIVVGAGAAGNAATYTLAKAGLNVLQIERGETSGTKNVQ
                     GAILYADALERIIPDFRDDAPLERHIVEQRIWIMEDKSHVGTHYRSDSFNEERPNRYT
                     ILRAQFDKWFSGKVRESGALVINETTVLELLKNDAGRIVGVRTDRVGGDIYADAVILA
                     DGVNSLLARKAGLREEIEAKNVALAVKEVHFLPQDVLESRFNIQEEEGVVIEIAGTIT
                     RGMAGTAFLYTNRESVAIGIGCMLEDFKGSGVSPYTLLEDLKNHPSIKSLIAGGEVKE
                     YAAHLIPEGGYKALPKLAGDGWLMVGDSAGFVNAVHREGSNLAMTSGRLAAETLIALK
                     TAGKPATAANLQAYVEALNDSFVMKDLRKYQHVPALLEEKQFLTTYPTLLNRAAETML
                     RVDGVDKKTKQRSALGAFVQDRSLFGLMKDTYKLWRAFR"
     gene            4008..4295
                     /locus_tag="PLQ18_00550"
     CDS             4008..4295
                     /locus_tag="PLQ18_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014029007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HNE00164.1"
                     /translation="MSKVEEKLFQNRYRVDEGRPHIQIIDQEKCTECGTQDCTYLCPA
                     ACYAKAEDGRHVVLTTDGCLECGTCRVICSEFRNVAWEYPRGGYGILFKFG"
     gene            4306..4860
                     /locus_tag="PLQ18_00555"
     CDS             4306..4860
                     /locus_tag="PLQ18_00555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE00165.1"
                     /translation="MLTPSLPLRAVPLAEYPFGLGVTDGAGFRALREAAADDAASVAG
                     AYLEQAAAGVEPARREFLLWSAHALAPNHLPVYFALYKFLFHQKRLVDAERVALMALG
                     ESARQGGFPVRWNELRHWTADWSDGPGRFYLFSLKALAFIRLRLHGLKAAGPLLEKLH
                     ELDPQDQVGGSVITDLARACSAAG"
     gene            4976..5434
                     /locus_tag="PLQ18_00560"
     CDS             4976..5434
                     /locus_tag="PLQ18_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HNE00166.1"
                     /translation="MDCINRFLAHAIALEGSAAGRYEDLANSMSALGDAEVAAFFRQM
                     AEFARMHQAEAIDRAGFRDVEQPPEDGYEWGPGESPEDAAWAGVDSFLDVTAALELAL
                     EAERSSEAFYRRVARETSDAEVRGFAESFAEEEVGHVNALLARLERRASV"
     gene            5550..6077
                     /locus_tag="PLQ18_00565"
     CDS             5550..6077
                     /locus_tag="PLQ18_00565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE00167.1"
                     /translation="MAGSGDGREPRSRRGKNGCHPLKKIHLNQQPDEINASFSPAGIT
                     PAHTGSSQPGRPKAPAADAASPPPSHPPALKSEPEMSTMNLNIRTLIATALISFAAAG
                     SVNALASEADSLPMPSWNPEQTVYGSPIGLSQAPAAMTRFEARMSATGSGHELCAARV
                     ALNPNAGRNASLQAC"
     gene            6186..6500
                     /locus_tag="PLQ18_00570"
     CDS             6186..6500
                     /locus_tag="PLQ18_00570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE00168.1"
                     /translation="MRGFASSLARAALPAALVLASAIGSIALAAGPAAVPSRDAALLA
                     PQLPPALRDTDPALPTLTSPDAAPARRPAADNGRVGDDTTFCNVFLNESERERSGATG
                     FC"
     gene            6631..6900
                     /locus_tag="PLQ18_00575"
     CDS             6631..6900
                     /locus_tag="PLQ18_00575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE00169.1"
                     /translation="MNLKTAIAASLLTLSVATIAHAGERDSYPQPSWDPDTSDHLHRP
                     AAPLGAANASIDERMRATGSSTDFCITELYPLNPRGGNNSHAGIC"
     gene            7003..7287
                     /locus_tag="PLQ18_00580"
     CDS             7003..7287
                     /locus_tag="PLQ18_00580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE00170.1"
                     /translation="MNVKTAIAASLLTLSIAGAAHAMDRYDTGSRPGAYPEYPSETGS
                     GMHQRAPVTQSGSPGFDQRMRATGSGFDYCQQALYPLEPSSGRNVLGGTC"
     gene            7363..7632
                     /locus_tag="PLQ18_00585"
     CDS             7363..7632
                     /locus_tag="PLQ18_00585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE00171.1"
                     /translation="MNLKTAIAASLLTLAAATAAHAGDSDSYPRPDWNPNASDYLYKQ
                     PNPYGMSTAASIDERMRATGSGTEFCVEPYALNPRDGSNSHAGVC"
     gene            complement(7864..>8906)
                     /locus_tag="PLQ18_00590"
     CDS             complement(7864..>8906)
                     /locus_tag="PLQ18_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNE00172.1"
                     /translation="LLIVPPWINKFYILDLRDKNSFIRWAVAQGNTVFVISWVNPDAS
                     YAARGFDDYLEHGITAALQAIERATGEREVNAVGYCLGGTLLSASTAWYAAGDHQPIA
                     STTLFTTMLDFARPGELEVFIDDQQLDAIERRMQKQGYLDGAEMASTFNMLRANDLIW
                     SFAVNNYLLGKDPFPFDLLYWNSDSTRMPAAMHSFYLRNMYQHNRLREPGGITLCGRP
                     IDLRQVRTPVYFLSAVEDHIAPWKSTYAGTRLFGGPVRFVLGGSGHIAGVINPPAAKK
                     YGYLTSPRLELDADEWLATATQTAGSWWPDWDAWLDQQDATRVPARHAGDGALAVLEP
                     APGSYVKRRLAR"
BASE COUNT         1514 a         3224 c         2850 g         1318 t
ORIGIN      
        1 cgccatcgag atcggcgccg gtgcgcgcgt cggggcgaat tcggtcgtga tccgcgacgt
       61 gccggccgcg gccaccgtcg tcggcatccc cggtcgcgtc gtgccgcgcg gcgacacggc
      121 ccgggccgac ggcatcgacc tcgatcacca cctgatgccg gacccggtcg gccgtgcgct
      181 cgcctgcctg atccagcgca tcgacgacct cgaagcccgg ctcgccgccg ccggtccggc
      241 catccccgga ctcgatcacc cgcatgcctg caccgactgc ggcgatgcct gcgatcaggc
      301 ccgccatgcc gccgccgccc accccgctgc cctgcactcc tgaggaatcc atccatgagc
      361 acgctgagcg atcgtctgca gaccctgtcg gccgccgatg aattcttcga gctgttcaag
      421 atcgacgtcg acccgcacgt cgtgcacgtc aaccgcctgc acatcctcaa gcgcttccac
      481 gactacctga agcgctcgcc ggtccaggcg gacctctccg aagccgacga attcgccgcc
      541 taccgcgagc ggctggtgca ggcctacctc gatttcaccg tgtcggacgc ggtcaccgag
      601 aaggtcttca aggtgttccg tcaggcgcag ggccaaggct tcgtcggtct cgaccagatc
      661 gaacgcatcg aacgacctgc gacctgattt ccccttatcc acgactgcca cgtacccgac
      721 gagccggagg ccacccatgc acatcgtcgt ctgcatcaag caggtaccgg acagcgcgca
      781 gatccgcgtg cacccggtca ccaacaccat catgcgccag ggcgtcccgg ccatcatcaa
      841 cccctacgac ctgttctcgc tggaagaggc gctgcggctg aaggaccagt tcggcggcaa
      901 ggtgacggtg atcacgatgg gtccgccgca ggccgaggat gcgctgcgca aggccctgtc
      961 cttcggcgcc gacgaggcga tcctgctgac cgaccgcgcg tttgccggtg ccgacacgct
     1021 ggcgacctcg tgcgcactgg cccaggccct gcgccagatc ggcgagaagg agccggtcga
     1081 cctggtgttc accggcaagc agacgatcga cggcgacacc gcccaggtcg gcccgggagt
     1141 ggccaagcgc ctcgacctgc agctgctgac ctacgtcgcg aagatcgacg agctcgacct
     1201 cgcaggccgc acgatcaccg tgcagcggcg cgccgagggc ggcgtgcagg tgctcaagac
     1261 ccgcctgccg gcgctgatca cgatgctcga aggcaccaac gagatgcgct tcgcgaccat
     1321 gaccgacatg ttccgcgccg cgcgctcgcc gctgacggtc tggaacaagg acgacgccgg
     1381 catcaccgac atcagcaaga tcggcctgaa gggctcgccg accgtggtca gcaaggtgtt
     1441 cgcgccgaag ccgaagtcgg tgccggcgca gatgctcgac atcggcggca agagccagac
     1501 cgagatcgcc gacctgatca tcgacaccct gtccgcgaag catgcgccgc tgtacggcga
     1561 gctgttcgac cgcgcgctct gaaccccacg actcccggag atagccgaca tgagcgaagc
     1621 cccgaagccg gccgcccccg cagcgcgcgg ccgcaagaaa ctcgaactgg acccgcggct
     1681 ggccgcgtac aagggtgtgt gggtgttcat cgagcatgaa cgcggcaccg tgcacccggt
     1741 gtcgtgggag ctgctcggcg aaggccgcaa gctcgccgac aagctcggcg tggagctcgc
     1801 cggcgtgctg ctcggcggcc ccggggatcc gctcgatgcc ttcgccgcca ccgcccacac
     1861 ctatggcgcc gacctcgtct acatcacccg cagcccggtg ctggccggct accgcaacga
     1921 gccgtacacg aaggcgctga ctgcggtcgt caaccagttc cagcccgaga tcctgctgct
     1981 cggcgccacc gtgcagggcc gtgacctcgc cggttccgtg gcgacgacgc tggcgaccgg
     2041 cctgaccgcc gactgcaccg aactcgcgat cgatccggac aaccgctcgc tcgccgcgac
     2101 ccgtccgacc ttcggcggct cgctgctctg cacgatccag acgctgaact accggccgca
     2161 gatggcgacc gtgcgtccgc gcgtgatgtc gatgccggcg cagcagcccg agcgcagcgg
     2221 ccgcgtgctt gaggcgccgg tcgagctggt cgaggacgac atcatcgcca agctgctttc
     2281 gtacatcccg gacgatgcca ccgaccaggc gcagctcgcc tacgccgacg tgatcgttgc
     2341 cggcggccgc ggcctcggca agcccgagaa cttcaagctg ctctgggatc tggccgaggt
     2401 gctcggcgcc gaggtcggcg gctcccggcc ggtgatccag aacggcatcc tcagtgcgga
     2461 ccgccaggtc ggccagaccg gcaagaccgt gcgcccgaag ctctacatcg ccgccggcat
     2521 ctccggcgcg atccagcacc gcgtcggcat ggaagcctcc gacgtcatca tcgccatcaa
     2581 cacggacccc aaggccccga tcttcgactt cgccacctac ggcatcgtcg gtgatgcggt
     2641 gcagatcctc cctgccctga ccgcagcctg ccgcgcccgt ctggcgatga agcgcgccgg
     2701 ctgaaggaga ccgccatgga aaaattcgac gtcatcgtgg tcggggccgg cgctgccggc
     2761 aatgccgcca cctacaccct ggccaaggcc ggcctgaacg tgctgcagat cgagcgcggc
     2821 gagacctcgg gcaccaagaa cgtgcagggc gcgatcctgt acgccgatgc gctggagcgc
     2881 atcatcccgg acttccgcga cgacgcgccg ctcgaacgcc acatcgtcga gcagcgcatc
     2941 tggatcatgg aggacaagtc gcacgtcggt acgcactacc gctccgactc cttcaatgag
     3001 gaacggccga accgctacac gatcctgcgc gcgcagttcg acaagtggtt cagcggcaag
     3061 gtcagggagt ccggcgcgct ggtcatcaac gagaccacgg tgctggagct gctgaagaat
     3121 gacgccggcc gcatcgtcgg cgtgcgcacc gaccgcgtcg gcggcgacat ctacgccgat
     3181 gccgtgatcc tcgccgatgg cgtcaactcg ctgctggccc gcaaggccgg gctgcgcgag
     3241 gagatcgagg cgaagaacgt cgcgctcgcg gtcaaggaag tccacttcct gccgcaggac
     3301 gtgctggaga gccgcttcaa catccaggaa gaggaaggcg tcgtcatcga gatcgccggc
     3361 accatcaccc gcggcatggc cggcacggcc ttcctgtaca ccaaccgcga gtcggtcgcg
     3421 atcggcatcg gctgcatgct ggaggacttc aagggctcgg gcgtgtcgcc gtacacgctg
     3481 ctcgaagacc tgaagaacca cccgtcgatc aagtcgctga tcgccggcgg cgaggtcaag
     3541 gaatacgccg cgcacctgat ccccgagggc ggctacaagg cgctgccgaa gctcgccggc
     3601 gacggctggc tgatggtcgg cgactccgcc ggcttcgtga atgccgtgca ccgcgaaggc
     3661 tcgaacctgg cgatgaccag cggccgcctg gccgccgaga cgctgatcgc gctgaagacc
     3721 gccggcaagc cggcgacggc cgcgaacctg caggcctacg tcgaggcgct caacgacagc
     3781 ttcgtcatga aggacctgcg caagtaccag cacgtgccgg ccctgctcga agagaagcag
     3841 ttcctgacca cctacccgac cctgctcaac cgcgccgccg aaaccatgct gcgcgtcgac
     3901 ggtgtcgaca agaagaccaa gcagcgctcg gcgctcggcg cgttcgtgca ggaccgctcg
     3961 ctgttcgggc tgatgaagga cacctacaag ctgtggaggg cattccggtg agcaaggtcg
     4021 aggaaaagct gttccagaac cgctaccgcg tcgatgaagg ccgcccgcac atccagatca
     4081 tcgatcagga gaagtgcacg gaatgcggta cccaggactg cacgtatctg tgcccggccg
     4141 cctgctacgc caaggccgag gacggccggc atgtggtgct gaccaccgac ggctgcctgg
     4201 aatgcggcac ctgccgggtg atctgctcgg agttccgcaa cgtcgcctgg gaatacccgc
     4261 gcggcggcta cggcatcctg ttcaagttcg gctgaggaca aggccatgct gaccccgtcc
     4321 ctgccgctgc gtgcggtccc gctcgcggag tacccgttcg gactcggcgt caccgatggc
     4381 gccggcttcc gggcgctgcg cgaggcggca gcggacgatg ccgcctcggt tgccggtgcc
     4441 tacctcgaac aggcggcggc tggcgtcgag ccggcccgcc gcgagttcct gctctggagc
     4501 gcgcacgcgc tggcaccgaa tcatctgccc gtgtacttcg cgctgtacaa gttcctgttc
     4561 caccagaagc ggctggtcga tgccgaacgc gtggcgctga tggcgctcgg cgagtcggcc
     4621 cgccagggcg gcttcccggt gcgctggaac gaactgcgtc actggaccgc ggactggtcc
     4681 gacggcccgg gccgcttcta tctgttcagc ctgaaggcgc tggccttcat ccggctgcgg
     4741 ctgcacggcc tgaaggccgc cggaccgctg ctggagaagc tgcacgagct ggatccgcag
     4801 gaccaggtcg gcggctcggt gatcaccgat ctggcccggg cctgcagcgc agcgggctga
     4861 gccggaccac gaatcgacac gctggaccgg tatcctcacg cccggtcccg cttccgtcgc
     4921 cggccgatcc atggccgcag cgacgcgcct ttcctacccg acggtggagt acttcatgga
     4981 ctgcatcaac cgttttctcg cccacgcgat cgcgctcgaa ggctcggccg ccgggcgcta
     5041 cgaagacctg gccaactcga tgagcgcgct cggtgacgcc gaggtcgcgg cctttttccg
     5101 gcagatggcc gagttcgccc gcatgcatca ggccgaggcc atcgaccgcg cggggttccg
     5161 cgatgtcgag cagccgcccg aggacggcta cgaatggggg ccgggcgaaa gccccgagga
     5221 cgccgcctgg gccggcgtcg acagcttcct cgacgtgacc gcggcactgg aactcgcgct
     5281 cgaagccgag cgcagcagcg aggccttcta ccggcgcgtc gcgcgcgaga cctcggatgc
     5341 cgaggtgcgc ggcttcgccg aatccttcgc cgaggaggaa gtcggccacg tcaacgcgct
     5401 gctcgcccgg ctggagcggc gcgcgtcggt ctgatcccgc cccgcgcact ccgcccccga
     5461 tcaccccggc ccccgtgccg gggtttttct ttgcatgccc cggccgggtc gcgggccgcg
     5521 accggccact gccgccgccg cgacatgccg tggccggcag cggcgatggc cgggagcccc
     5581 gcagccggcg agggaaaaac ggctgccatc cactcaaaaa aatccattta aatcagcaac
     5641 ctgatgaaat taacgccagc ttttctccgg ctggcatcac tcctgcccat accggttcat
     5701 cccagcccgg acgcccgaag gcgccggctg cggacgcggc aagcccgccg ccatcccatc
     5761 cgcctgccct gaagtccgaa ccggagatga gcaccatgaa cctgaacatc cgcaccctga
     5821 tcgccaccgc cctgatcagc tttgccgccg ccggcagcgt caatgcgctg gccagcgaag
     5881 ccgacagcct gccgatgccg tcctggaatc ctgagcagac ggtgtacggc agcccgatcg
     5941 gcctgtcgca ggctccggcc gcgatgaccc gcttcgaggc acgcatgagc gcgaccggca
     6001 gcggccacga gctctgcgcg gcacgcgtcg cgctgaaccc gaacgccggg cgcaatgcat
     6061 ccctgcaggc ctgctgaacg gctcccgcag cggttcacgc cggccgatgc gggaaccttt
     6121 gccgcggcag cggcatcgga gcaggcgtta cccgcccgac ggactgcctc tgcgaggtac
     6181 caatcatgcg aggtttcgcc tcttccctgg cccgcgccgc cctgcccgcc gccctggtgc
     6241 tcgcgagtgc catcggcagc atcgccctcg ccgccgggcc tgccgccgtc ccgtcccgcg
     6301 acgccgcgct gctcgccccg cagctgccgc cggcgctgcg ggacaccgac cccgccctgc
     6361 cgacgctgac gagtccggac gcggctccgg cgcgccggcc ggcggccgac aacggtcgcg
     6421 tcggcgacga cacgactttc tgcaacgtgt tcctgaacga atcggagcgc gagcgcagcg
     6481 gcgcaaccgg cttctgctga gccctgccgg cggccggccg gaaaaatttc cgccccggcc
     6541 gcatccgccg cgctccctgc agcgcatatc gccctgcaag ccccgggaat ccaagccccg
     6601 cgaacccacc cgagagacga gaaaaccgcc atgaacctca agaccgccat cgctgccagc
     6661 ctgctgaccc tttccgtcgc caccatcgcc catgccggcg agcgcgattc ctatccgcag
     6721 ccgtcctggg atccggacac cagcgatcac ctgcaccgcc cggccgcacc gctcggtgct
     6781 gcgaatgcct cgatcgacga gcgcatgcgt gccaccggca gcagcaccga cttctgcatc
     6841 accgagctgt atccgctgaa tccgcgcggc ggcaacaaca gccacgccgg catctgctga
     6901 accggtaccg gcgctgtgga ggtaccgcga gccgctgcag cgccggtttc cgttcccccg
     6961 aggctcacga gccttccccc cgaggcaaac gaggactccg acatgaacgt caagaccgcc
     7021 atcgccgcca gcctgctgac cctgtccatc gccggtgctg cccatgccat ggaccgttac
     7081 gacaccgggt cacggcccgg cgcctatccg gaatatccgt ccgagaccgg cagcggcatg
     7141 caccagcgcg ccccggtcac acagagcggc agccccggct ttgaccagcg catgcgtgcc
     7201 accggcagcg gcttcgatta ctgccagcag gcgctgtacc cgctggagcc gagcagtggc
     7261 cgcaacgtgc tcggcggtac ctgctgatct gcattcatga cggcgcgggc ccgagtcgcc
     7321 cgcgccggcc ctgccgcctg aaagcctcac gaggacactg ccatgaatct gaagaccgcc
     7381 atcgccgcca gcctgctgac ccttgccgcc gccacggctg cccatgccgg cgacagtgac
     7441 tcctatccgc gtccggactg gaacccgaac gccagcgact acctgtacaa gcagccgaac
     7501 ccgtacggga tgagcacggc tgcgtcgatc gatgaacgca tgcgcgcaac cggcagcggg
     7561 acggaattct gcgtcgagcc gtatgcgctg aatccgcgcg acggcagcaa cagccacgcc
     7621 ggcgtctgct gatcgccctg cctgacttcc tttctcccga tccgggtttt ccgttcacgc
     7681 ggaacccgga tttttttatg aactgcatcc gatgcgtgca gcgcgcccaa aaaaataccg
     7741 ccgctccggt ttaaggaggc ggcggttgcg gtgtgccggc ggcatccggt cttatgagcc
     7801 ggcgggcacc gtatggctgc gggcggtggg acgggacaga acctgaatca ggcagtctgg
     7861 aactcagcgc gcgagccggc gcttgacgta gctgcccgga gccggttcta gcacggcgag
     7921 cgcaccatcg ccagcgtggc gtgccggtac gcgcgtcgca tcctgctgat ccagccaggc
     7981 atcccagtcc ggccaccacg atccggcggt ctgcgtggcc gtggccagcc actcgtcggc
     8041 gtcgagctcc agacgcggac tggtcagata gccgtacttc ttcgcggccg gcgggttgat
     8101 gacaccggcg atgtggccgg aaccgccgag cacgaaacgc accggcccgc cgaagaggcg
     8161 cgtgccggca tacgtggatt tccacggcgc gatgtggtcc tcgaccgccg acaggaaata
     8221 caccggcgtg cgcacctgac gcaggtcgat cggccggccg cacagcgtga tgccgccggg
     8281 ctcgcgcagg cggttgtgct gatacatgtt gcgcagataa aaactgtgca tcgccgccgg
     8341 catgcgcgtc gaatccgagt tccagtacag caggtcgaac ggaaacggat ccttgccgag
     8401 caggtagttg ttgaccgcga atgaccagat caggtcattg gcgcgcagca tgttgaaggt
     8461 cgacgccatc tcggcgccgt cgagatagcc ctgcttctgc atgcgcctct cgatggcatc
     8521 gagctgctgg tcgtcgatga acacttccag ttcgccgggc cgggcgaaat cgagcatggt
     8581 cgtgaacagc gtggtgctgg cgatcggctg atgatcgccg gccgcatacc aggccgtgct
     8641 cgccgacagc agcgtaccgc cgaggcagta gccgacggca ttgacctcgc gctcgccggt
     8701 cgcccgttcg atggcctgca gcgccgccgt gatgccgtgt tcgaggtaat catcgaaacc
     8761 gcgggccgcg tagctggcat ccggattgac ccaggagatc acgaacaccg tgttgccctg
     8821 cgcgacggcc cagcggatga aactgttctt gtcgcgcagg tcgaggatgt agaacttgtt
     8881 gatccacggc ggcacgatca gcagcg
//
LOCUS       DAOQJH010000011         8729 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_4317_length_8729_cov_4.662094, whole genome shotgun sequence.
ACCESSION   DAOQJH010000011 DAOQJH010000000
VERSION     DAOQJH010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 8729)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8729)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8729
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4317_length_8729_cov_4.662094"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..933)
                     /locus_tag="PLQ18_00595"
     CDS             complement(<1..933)
                     /locus_tag="PLQ18_00595"
                     /inference="COORDINATES: protein motif:HMM:NF012708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNE00173.1"
                     /translation="MTLRITSQHPLLPEADTVRSLGSGDSLTIGRAPGNDWVLPDPEL
                     VISGRHCTVHEEQGRYVLTDTSTNGVYLGGQRVGRGNSRPLQAGDVIAIGEYQIAVEP
                     GAGDGGQAPAFGAGTAAAVALPDAFGLAAPAPLADPFGLHGTPGAQDPFELHGKPAGE
                     DPFGLHAPATGDDPFGLGNDLSGGDPFGLIGTPGAGGSARGGGTPAAADPFGLAGGGD
                     RNPAGADPFGLGGGAGRPDFAAPATPAGPATAEAGGDPFGLLAPAPLTSAPVPDHVPS
                     EQQAFVPPAPVSDDDWDALFPAPAAAGAAPVRLDI"
     gene            1146..2225
                     /gene="tssA"
                     /locus_tag="PLQ18_00600"
     CDS             1146..2225
                     /gene="tssA"
                     /locus_tag="PLQ18_00600"
                     /inference="COORDINATES: protein motif:HMM:TIGR03363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HNE00174.1"
                     /translation="MALDVEKWLEPVSDDEPGGSDLEYDPDFQALERLATGKPEQQIG
                     STIVPAEPPDWRAVRGKAMELLERTRDLRVALVLTRALLAVEGLPGFADGLRYLHGLL
                     ERHWEHVHPQLDPDDYNDPTTRVNIIAGLVAADSDDVLGLLRQAALARSRSFGVATLR
                     DWLIRSGASSGTPVLTAETFDAVFREVAAEELQAAADSAHAAFDLASAIERRLTEQVG
                     SGNAVDLSPLRTALKPCVQLLDERLAQRGLGAVAETGALPVAGEEPAEAGGSAATGAG
                     ASGFVAGRISTRDDVLKALEAVCDYYTRHEPSSPVPILMERARRLVTMSFVDIMRNIA
                     PDSLAQIDVLRGPDPDAESSSSDGY"
     gene            2296..2796
                     /gene="tssB"
                     /locus_tag="PLQ18_00605"
     CDS             2296..2796
                     /gene="tssB"
                     /locus_tag="PLQ18_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007040994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HNE00175.1"
                     /translation="MADSSQKFIARNRAPRVQIEYDVELYGAQKKVQLPFVMGVMADL
                     SGKPAEPLGPVAERKFLEIDVDNFDSRMKSMKPRVAFAVPNTLTGDGNLSVDITFESM
                     EDFTPAAIARKVDALNKLLEARTQLSNLLTYMDGKTGAEELIGKVLQDPSLLQALASA
                     PKPQSE"
     gene            2815..4311
                     /gene="tssC"
                     /locus_tag="PLQ18_00610"
     CDS             2815..4311
                     /gene="tssC"
                     /locus_tag="PLQ18_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014778682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HNE00176.1"
                     /translation="MAELNAEAQAAGAGLTTEGGAFEALLLQEFKPKSDRAKEAVEEA
                     VRTLAEQALSSSSIVSGDTLKTIEAMIAALDAKLTEQINHILHHADFQKLESAWRGLH
                     HLVNNTETDEMLKIRVMNISKQDLHKTLKKFKGTAWDQSPIFKKLYEEEYGQFGGEPY
                     GCLVGDYYFDHSAPDVELLGEIAKVSASMHAPFIAAVAPAVMQMDSWQELSNPRDLTK
                     IFQTPEYAAWKSLRESEDSRYVGLTMPRFLSRMPYGAKTNPVDEFDFEEDTTGADSNK
                     YTWANSAYAMAVNINRSFKLYGWCSRIRGIESGGAVEGLPAHSFPTDDGGVDMKCPTE
                     IAISDRREAELAKNGFMPLVHKKNTDFAAFIGAQSLQKPAEYDDPDATSNANLAARLP
                     YLFATCRFAHYLKCMVRDKVGSFKEREDMQIWLQKWIMNYVDGDPSRSSETTKAQKPL
                     AAAEVVVEEVEGNPGYYTSKFFLRPHYQLEGLTVSLRLVSKLPSQKGG"
     gene            4420..4914
                     /locus_tag="PLQ18_00615"
     CDS             4420..4914
                     /locus_tag="PLQ18_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019848570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
                     /protein_id="HNE00177.1"
                     /translation="MAVDIFLKFADAANIKGESKDKKHPKEIDVLAWSWGMSQSGTFH
                     TGGGGGAGKVNVNDMSVTKWVDIASYALMHACATGQHIDDALLTVRKAGGKEPVEYIK
                     IKMEKVMVTSINTGGSGGEDRLTENVSLNFAKVTFTYTEQTDKGAGEASKDFKWDIEA
                     NAVP"
     gene            5054..5857
                     /locus_tag="PLQ18_00620"
     CDS             5054..5857
                     /locus_tag="PLQ18_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013817560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system accessory protein TagJ"
                     /protein_id="HNE00178.1"
                     /translation="MSVEAALQSGNPREALTELQARVRKEPAVARHRVLLFQLLCVLG
                     QWPRALGQLQVVGELDAEALPMVQTYREALRCEVFRQEVFAGRRAPLLFGEPTPWVAL
                     LIEALARDADDPQAAAGLRAEAFERAPANPGSADGRPFAWLADTDGRLGPVIEAIING
                     RYYWIPCERLSTLQQEAPADLRDVVWMPASLTFTTGGETVALLPARYPGLDAEDDGGL
                     LLGRRTEWRENEHGLVLAAGQRVFATDGDDVALYDLRTLHFDPAAPASA"
     gene            5866..6369
                     /gene="tssE"
                     /locus_tag="PLQ18_00625"
     CDS             5866..6369
                     /gene="tssE"
                     /locus_tag="PLQ18_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013817561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HNE00179.1"
                     /translation="MAELTPQERLQPALLDRLIDDAPERHKETREQRVLSLNQLRESV
                     LRDLAWLFNAGNHESVQPLDAWPQVARSVLNYGLPALSGRTASSVDIPALERVLADTI
                     RTFEPRILPHTLQVRAVVAREQMNHNAIGFEIQGELWAQPVPLHLWLKTEIDLESGNV
                     RIEPRNA"
     gene            6387..8264
                     /gene="tssF"
                     /locus_tag="PLQ18_00630"
     CDS             6387..8264
                     /gene="tssF"
                     /locus_tag="PLQ18_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019372512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HNE00180.1"
                     /translation="MDPRLYRYYNQELQHLRETAGEFAQEFPKIASRLSLTGFECADP
                     YVERLLEGCAFLSARVQLKLDAEFPVFTQHLLEMVYPHYLAPVPSMAVLQLQPDLSEG
                     SLAAGFTVPRGASLKSVLGKNETTACEYRTGQPVTLWPVELTEAEYFGNVTAVLGPAA
                     NSAPRARAGLRLRLRATAGLSFDALSLERLPLFLRGADQLPFRLYEQLLGNAVAVLAR
                     PAQRPAPWFQWLGRESLAPMGFEDEQALLPLSPQSFQGYRLLGEYFAFPQRFLFVELR
                     ALAPVVRRCSGSELELLVLFDRLDPMLEGVLKPENFVLHCAPAINLFPRRADRIHLND
                     RSYEYHIVPDRTRPLDFEVHSLERVTGHGTTEQAEQAFLPFYCAHDRIRRAADGAYYT
                     LRRTKRLLSARAKLRGPRSGYIGQEVFIALVDAAAAPYRHDLRQLSLEVLCTNRDLPL
                     TMPLGLGRTDFTLESGAPVAAVRCVAGPTRPRPSNAENDVAWRLISHLSLNYLTLADS
                     DAQQGAVALRELLRLYVDPNDAAGQKQVDGLLSVKTQPATRRLPLPGPIAFGRGVEIA
                     LNFDEGAYGGAGVFLMGAVLERFLGRYVSMNSYTETSVRSLERGEIMRWPVRMGARAL
                     L"
     gene            8339..>8729
                     /locus_tag="PLQ18_00635"
     CDS             8339..>8729
                     /locus_tag="PLQ18_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HNE00181.1"
                     /translation="MLLSPVAEALFTALAAKPWDFDLWQALRRIECAFPDKPRLGRSF
                     RVADDPVRLSQPPHTTFVPSTIAAFERPSHRPARLASYAFGLFGPHGPLPLHLSEHAR
                     ERQRNHRDPTFARFADIFHHRLLLLFFR"
BASE COUNT         1399 a         3079 c         2957 g         1294 t
ORIGIN      
        1 aatgtccagc cgcaccggcg ctgcgccggc agcggccggg gccggaaaca gtgcatccca
       61 gtcatcgtca ctgaccggcg ccggcggcac gaaggcctgc tgctcggacg gcacgtgatc
      121 cggaaccggg gcagacgtga gcggcgccgg cgccagcagg ccgaacggat caccgccggc
      181 ttcggccgtc gcggggccgg ccggcgttgc cggcgctgcg aaatccggcc gtccggcgcc
      241 gccgccgagc ccgaacggat cggcgcccgc cggattgcgg tcaccgccgc cggcaagacc
      301 gaacggatcc gcggccgcgg gcgtgccgcc accccgggcg ctgccgccgg cgcccggcgt
      361 gccgatcagt ccgaaggggt cgccgccgga caggtcattg ccgagcccga acggatcatc
      421 accggttgcg ggcgcatgca gcccgaacgg atcctcgccg gccggcttgc cgtgcagttc
      481 gaacgggtcc tgcgcacccg gcgtgccgtg caggccgaac ggatcggcga gcggtgccgg
      541 tgcggcaagg ccgaaggcat ccggcagcgc cacggccgct gccgtgccgg ccccgaaggc
      601 cggggcttgt ccgccgtcgc cggcgcccgg ctcgacggcg atctggtatt cgccgatggc
      661 gatcacgtcc ccggcctgca gcggccggct gttgccgcga ccgacgcgct ggccgccgag
      721 atagacgcca ttggtgctgg tgtcggtcag cacgtagcgc ccctgctcct cgtgcacggt
      781 gcagtggcgg ccggagatga ccagctccgg gtccggcagc acccagtcgt tgccgggcgc
      841 acggccgatc gtcaggctgt cgccgctgcc gaggctgcgc acggtatcgg cctcgggcag
      901 gagcgggtgc tggctggtga tacggagggt caacggcatc ggtcgtgaaa cctgggcggc
      961 aagccggggg gcgaaaagcc cgccgactgt aggccagtgc cggcacggct gacaattcgc
     1021 cgctcgctgc cggctggcag tccgccggcc gccggctacg ctacgcagcg accgcactgg
     1081 cggacgccac gatgcgcggc gccggccggt gcgcagatcg ggtgacaatc gcagggggtg
     1141 ccccggtggc gctggatgtg gagaaatggc tcgaacccgt ctcggacgac gagcccggtg
     1201 gcagtgatct cgaatacgat cccgatttcc aggccctcga acggctcgcg accggcaagc
     1261 ccgagcagca gatcggcagc accatcgtgc cggccgaacc gccggactgg cgggccgtgc
     1321 gcggcaaggc gatggaactg ctcgaacgca cgcgcgacct gcgcgtcgcg ctggtgctga
     1381 cccgcgcgct gctcgccgtc gaggggctgc ccggattcgc cgacggcctg cgctatctgc
     1441 acggcctgct ggagcgccac tgggagcacg tgcatccgca gctcgatccg gatgactaca
     1501 acgatccgac cacgcgcgtg aacatcatcg ccggcctcgt cgccgccgac agcgacgacg
     1561 tgctcggcct gctgcgccag gccgcgctgg cgcgctcgcg cagcttcggc gtcgcgacgc
     1621 tgcgcgactg gctgatccgt tccggcgcga gcagcggcac gccggtgctg accgccgaga
     1681 ccttcgatgc cgtgttccgc gaggtcgccg ccgaggaact gcaggcggcc gccgacagcg
     1741 cgcatgcggc cttcgatctc gcctcggcaa tcgagcgccg cctgaccgag caggttggct
     1801 ccggcaacgc cgtggatctc tcgccgttgc gcacggcgct caagccctgc gtgcagctgc
     1861 tcgacgagcg cctggcccag cgcggcctgg gcgccgtggc cgagaccggg gccttgccgg
     1921 tggccggtga ggagccggcc gaggcgggcg gcagcgcggc gaccggggcc ggcgcctccg
     1981 gtttcgtcgc cgggcgcatc agtacgcgag atgacgtatt gaaagctctg gaggccgtct
     2041 gcgattacta cactcgacac gagccttcga gcccggtacc gatcctgatg gagcgcgcgc
     2101 gccggctggt gacgatgagt ttcgtcgaca tcatgcgcaa catcgccccc gacagcctcg
     2161 cgcagatcga tgtcctgcgc ggccccgatc cggatgccga atcgtccagc tcggacggct
     2221 actgacccgc tccccccgag ccggtgccga cgcgccgccg gttcgcgatc gcacaacgcc
     2281 tgaggagacc cgcccgtggc agacagcagt cagaaattca tcgcccgcaa ccgcgccccg
     2341 cgcgtgcaga tcgagtacga cgtcgagctc tacggcgccc agaagaaggt ccagctgccg
     2401 ttcgtgatgg gcgtcatggc cgatctgtcc ggcaagcccg ccgagccgct cggaccggtc
     2461 gccgagcgca agtttctgga aatcgacgtc gacaacttcg acagccgcat gaaatcgatg
     2521 aagccgcgcg tcgcgttcgc ggtgccgaac acgctgaccg gcgacggcaa cctgagcgtc
     2581 gacatcacct tcgagagcat ggaggacttc acgccggccg cgatcgcccg caaggtcgat
     2641 gcgctgaaca agctgctcga agcgcgcacc cagctctcca acctgctgac ctacatggac
     2701 ggcaagaccg gggccgagga actgatcggc aaggtgctgc aggacccctc cctgctgcag
     2761 gccctcgcgt ccgcgcccaa accgcagtcc gaatgacccc gaggtaacca gtccatggcc
     2821 gaactgaacg ccgaagccca ggccgcgggt gcgggcctga ccaccgaggg cggcgccttc
     2881 gaggcgctgc tgctgcagga attcaagccc aagagcgatc gagcgaagga agcggtcgag
     2941 gaggccgtgc gcacgctcgc cgagcaggca ctgtcgtcct cgagcatcgt ctccggcgac
     3001 acgctgaaga ccatcgaggc gatgatcgcc gcgctcgatg ccaagctgac cgagcagatc
     3061 aaccacatcc tgcaccacgc cgatttccag aagcttgaat cggcctggcg cggcctgcat
     3121 cacctcgtca acaacaccga aaccgacgag atgctgaaga tccgcgtcat gaacatctcc
     3181 aagcaggatc tgcacaagac cctgaagaag ttcaagggca cggcctggga ccagagcccg
     3241 atcttcaaga agctctacga ggaggaatac ggccagttcg gcggcgagcc ctacggctgc
     3301 ctggtcggcg actactactt cgaccattcg gcgcccgacg ttgaactgct cggcgagatc
     3361 gccaaggttt ccgcctcgat gcacgccccg ttcatcgcag ccgtcgcgcc ggcggtcatg
     3421 cagatggatt cctggcagga gctgtcgaac ccgcgcgacc tgaccaagat cttccagacg
     3481 cccgaatatg ccgcgtggaa gtcgctgcgt gaatccgagg actcgcgcta cgtcggcctg
     3541 accatgccgc gcttcctgtc gcgcatgccc tacggggcca agaccaatcc ggtcgatgaa
     3601 tttgatttcg aggaggacac gaccggggcc gacagcaaca agtacacgtg ggccaactcc
     3661 gcctacgcga tggccgtcaa catcaaccgc tcgttcaagc tctacggctg gtgctcgcgc
     3721 atccgcggca tcgaatccgg cggtgcggtc gaggggctgc ccgcgcattc cttcccgacc
     3781 gacgacggcg gcgtcgacat gaagtgcccg accgaaatcg cgatttccga ccggcgggag
     3841 gccgagctcg caaaaaacgg tttcatgccg ctcgtgcaca agaaaaatac cgatttcgca
     3901 gctttcatcg gagcccagtc gctgcagaag ccggccgagt acgatgatcc cgatgccact
     3961 tcgaatgcca acctcgccgc ccggctgccg tacctgttcg cgacctgccg cttcgcgcac
     4021 tacctgaagt gcatggtccg cgacaaggtg ggttccttca aggagcgcga ggacatgcag
     4081 atctggctgc agaaatggat catgaactac gtggacggcg atccctcgcg ctccagcgag
     4141 accacgaagg cccagaagcc gctcgccgcc gccgaggtcg tcgtcgagga agtcgaaggc
     4201 aaccccgggt actacacgtc gaagttcttc ctgcggccgc actaccagct cgaaggattg
     4261 acggtgtcgt tgcgactggt atccaagctg ccctcgcaga agggcggctg actggcggtg
     4321 cggtgactgc ggtcgccgca cctttgcaaa cggtccctga cgtccgggcg acgtaccggg
     4381 tcccaattcc agcattgacg cactgcagga gagacattca tggccgttga catctttctg
     4441 aaattcgcgg acgccgcgaa catcaagggc gaatcgaagg acaagaagca tccgaaggaa
     4501 atcgacgtcc tcgcctggag ctggggcatg tcgcagtccg gcaccttcca caccggcggc
     4561 ggtggcggcg ccggcaaggt caacgtcaac gacatgtcgg tgaccaagtg ggtcgacatc
     4621 gcctcgtatg cgctgatgca cgcctgcgcg accggccagc acatcgacga cgcgctgctg
     4681 accgtgcgca aggccggcgg caaggagccg gtcgagtaca tcaagatcaa gatggagaag
     4741 gtcatggtca cgtcgatcaa caccggtggc agcggtggcg aggatcggct gaccgagaac
     4801 gtctcgctga acttcgcgaa ggtcaccttc acgtacaccg agcagaccga caagggtgcc
     4861 ggtgaggcgt cgaaggactt caagtgggac atcgaggcca acgccgttcc gtaatccggt
     4921 ccgtctgcga acgccggctc gcggcccccg cccgcctgcc ggcctgcgct ccggcgcagg
     4981 tctgaattcc ggatttcccc gaatcccgga cgcgagccgc ccctgcaccg ggcggctggc
     5041 gtggaggtgt gtcgtgagcg tcgaagccgc cctgcagtcg ggcaatcccc gggaggcact
     5101 gaccgaactg caggcccgcg tacgcaagga gccggcggtt gcgcgccatc gcgtgctgct
     5161 gttccagctg ctctgcgtgc tcggccagtg gccgagggcg ctcggccagc tgcaggtggt
     5221 cggcgaactc gatgccgagg ccttgccgat ggtgcagacc taccgcgagg cgctgcgctg
     5281 cgaagtcttc cggcaggagg tcttcgccgg tcgcagggcg ccgctgctgt tcggcgagcc
     5341 gacgccgtgg gtcgcgctgc tgatcgaggc gctcgcgcgc gatgccgacg atccgcaggc
     5401 cgcggccggc ctgcgcgccg aagccttcga gcgggcaccg gccaatcccg gcagcgccga
     5461 cggccggccg tttgcgtggc tggccgatac cgacggccgg ctcgggccgg tcatcgaggc
     5521 gatcatcaac ggccgctatt actggattcc ctgcgaacgc ctgagcaccc tgcagcagga
     5581 ggccccggcc gacctgcgcg atgtggtctg gatgccggca tcgctgacct tcacgaccgg
     5641 cggcgagacg gtcgcgctgc tgccggcgcg ctacccgggg ctggacgcag aggatgacgg
     5701 cggcctgctg ctcggacggc gcaccgaatg gcgcgagaac gagcacggcc tcgtgctcgc
     5761 tgccgggcag cgggtgttcg cgaccgacgg cgacgacgtg gcgctgtacg acctgcgcac
     5821 gctgcacttc gatccggccg ctccggcgag cgcctgaggc tcaccgtggc tgaactgacc
     5881 ccgcaggaac gcctgcagcc ggcgctgctc gaccggctga tcgacgacgc gcccgagcgt
     5941 cacaaggaaa cgcgcgagca gcgcgtgctg tcgctgaacc agttgcgcga gagcgtgctg
     6001 cgtgatctcg cctggctgtt caacgccggc aaccacgaat ccgtgcagcc gctcgatgcc
     6061 tggccgcagg tcgcgcgctc ggtgctgaac tacggcctgc cggcgctctc cggacgcacg
     6121 gccagcagcg tcgacatccc ggcgctcgaa cgcgtgctcg ccgataccat ccgcaccttc
     6181 gaaccgcgca tcctgccgca cacgctgcag gtgcgcgccg tcgtcgcccg cgagcagatg
     6241 aaccacaacg ccatcggctt cgagatccag ggcgaactct gggcgcagcc ggtgccgctg
     6301 catctgtggc tgaagaccga gatcgacctc gaatccggca acgtccgcat cgagccccgc
     6361 aacgcctgat cgcaagcagc cccccgatgg atccgcgcct gtaccggtat tacaaccagg
     6421 aactccagca cctgcgcgag accgccggcg agttcgcgca ggaattcccg aagatcgcct
     6481 cgcgcctgag cctgaccggc ttcgaatgcg ccgaccccta cgtcgagcgg ttgctcgaag
     6541 gctgtgcctt cctgtcggcg cgcgtgcagc tgaagctcga tgccgagttc ccggtgttca
     6601 cgcagcacct gctggagatg gtctacccgc actatctggc gccggtgccg tcgatggcgg
     6661 tgctgcaact gcagccggac ctgtccgaag gctcgctcgc ggccggcttc acggtgccgc
     6721 gcggcgcgag tctcaagagc gtgctcggca agaacgagac caccgcctgc gaataccgca
     6781 ccggtcagcc ggtgacgctg tggccggtcg agctgaccga ggccgagtac ttcggcaacg
     6841 tcacggccgt gctcggcccg gccgcgaaca gcgcgccgcg cgcacgcgcc ggcctgcgcc
     6901 tgcgcctgcg ggccacggcc ggcctgagct tcgatgcgtt gtcgctcgag cgcctgccgc
     6961 tgttcctgcg cggcgccgac cagctgccgt tccggctgta cgaacagctg ctcggcaacg
     7021 ccgtcgccgt gctcgcccgc ccggcgcagc gtccggcgcc gtggttccag tggctcggtc
     7081 gcgaatcgct ggcgccgatg ggcttcgagg acgaacaggc gctgctgccg ctgtcgccgc
     7141 agagcttcca gggctaccgg ctgctcggcg agtacttcgc gttcccgcag cgtttcctgt
     7201 tcgtcgaact gcgcgccctg gcaccggtcg tgcgccgctg cagcggcagc gaactcgaac
     7261 tgctggtgct gttcgatcgg ctcgacccga tgctcgaagg cgtgctgaag cccgagaact
     7321 tcgtgctgca ctgcgcaccg gcgatcaacc tgttcccgcg ccgcgccgac cgcatccacc
     7381 tgaacgacag gagctacgag taccacatcg tcccggaccg cacgcggccg ctcgatttcg
     7441 aggtccactc gctcgaacgt gtgaccggtc acggcacgac cgagcaggcc gagcaggcct
     7501 tcctgccgtt ctactgcgca cacgaccgca tccgccgcgc cgccgacggg gcgtattaca
     7561 cgctgcgccg taccaagcgc ctgctgtcgg cgcgcgcgaa gctgcgcggg ccgcgcagcg
     7621 gctacatcgg ccaggaggtg ttcatcgcgc tggtcgatgc cgctgccgca ccgtaccggc
     7681 acgacctgcg ccagctgtcg ctcgaagtgc tgtgcaccaa ccgcgacctg ccgctgacga
     7741 tgccgctcgg gctcgggcgc accgacttca cgctggagag cggcgcgccg gtggcggcgg
     7801 tgcgctgcgt ggccggtccg acgcgcccgc gcccgagcaa cgccgagaac gacgtggcct
     7861 ggcggctgat ctcgcacctg tcgctgaact acctgacgct cgccgacagc gatgcgcagc
     7921 agggcgcggt ggcgctgcgc gagctgctgc gcctgtacgt cgatccgaac gatgccgccg
     7981 gccagaagca ggtcgacggc ctgctgtcgg tgaagacgca gccggcgacc cgccgcctgc
     8041 cgctgccggg gccgatcgcc ttcgggcgtg gcgtcgagat cgcgctgaac ttcgacgagg
     8101 gggcctacgg cggggccggc gtgttcctga tgggcgcggt gctggagcgc ttcctcggcc
     8161 gctacgtgtc gatgaacagc tacaccgaga ccagcgtgcg ttcgctggag cgcggcgaga
     8221 tcatgcgctg gccggtgcgc atgggcgcgc gggcgctgct gtgaacgccg acgccgacac
     8281 cgaggattcc ggcattgcgc cggcgctgag cgccgccgat ttcggcgtcg cgccgccggt
     8341 gctgctgtcg ccggtcgccg aggcgctgtt cacggcgctg gcggcgaagc cgtgggattt
     8401 cgatctctgg caggcgctgc ggcgcattga gtgcgcgttc ccggacaagc cgcggctcgg
     8461 gcgctcgttc cgggttgccg atgatccggt gcggctgtcg cagccgccgc acacgacctt
     8521 cgtgccctcg acgatcgctg ccttcgagcg tccgtcccac cggccggccc ggctggcgag
     8581 ctatgccttc gggctgttcg gcccgcacgg gccgctgccg ctgcacctga gcgagcacgc
     8641 gcgcgagcgc cagcgcaacc accgcgatcc gaccttcgcg cgctttgccg acatcttcca
     8701 ccaccggctg ctgctgctgt tcttccgcg
//
LOCUS       DAOQJH010000012         8687 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_4347_length_8687_cov_3.920992, whole genome shotgun sequence.
ACCESSION   DAOQJH010000012 DAOQJH010000000
VERSION     DAOQJH010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 8687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8687
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4347_length_8687_cov_3.920992"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..870)
                     /locus_tag="PLQ18_00640"
     CDS             complement(<1..870)
                     /locus_tag="PLQ18_00640"
                     /inference="COORDINATES: protein motif:HMM:NF022800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein N"
                     /protein_id="HNE00182.1"
                     /translation="MDEANRYSFGITRLRPGASRQGRSVHIACGLVAMLMAVDMATLV
                     TAQVPGASKVSGTDAGGPVVTQPVPEPLGRYALFGVAESPRDTPPPAAALKPSARPQL
                     RLTGTLAGAGHGLALISVGGQAEQAYPVGGTLASGWRLTAVFADRVQIAGFGESIEVP
                     MPAGESALARTSAPRDPAPAVSGVPAFPAAADDHEFSEDDPQAGSARASGRQERTPVD
                     ARPIARRLRGAPQPDAALRQLAGFDPVSLADHHGYGLRLRLGPESYLLTRAGLREGDV
                     IVAIGGVLLQDGAQ"
     gene            complement(1106..4480)
                     /locus_tag="PLQ18_00645"
     CDS             complement(1106..4480)
                     /locus_tag="PLQ18_00645"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HNE00183.1"
                     /translation="MNLVEKSAVRVIMTGPRWVINNFGLMVLMPILLLSVPCLSRAAE
                     VSFTNVTQAAGIAYIGESWGGAWGDFNNDGYLDIWLTNHRMPTHLYRNNKNGTFTNVA
                     SQVLKNYNFDGDNHGAVWVDIDNDGDQDLVELTGAAGGVATTSPNHDANLLVNAGGVL
                     EDRAVEMNFSNPYLRGRMPLPYDFDNDGILEIMQTGDIRSDGLAFPSVWKSNSHGYDD
                     ITDQCGAFPGAIAFAQSAAFPQGKTVLLYGGGASPSKAYNIESMPWVDNSAAFLEWQQ
                     KSVVVIDAAVADFNNDLNSEFYVARNNQLGIDYAEVKPLGNILFKVTVSPQKGETKKL
                     VFSADGSVDFDLVAPNHNLKQIFIGSAGRNPAQKHFVLSPNDAANQGLPQFSSNVPPG
                     LYVGFDPATHLWSLVNHTPATDRIIGYAVAGNIRATNIAIRDMSSLRTQATKLTDLLF
                     MYDPRQGRHVVTSWGFDASTPTMAVAVADFDNDMDVDIFLLKANNVKNFANQLWLNDG
                     SGHFVAAPDAAGLNMSVQGAGEAVIVADYDNDGFMDVLITNGYGEPPFNQGPVELFRN
                     SGNANHWLELNLVGGPSNRDAIGARIIATTPDGRKQLREVGEELHNHGQNMRRVHFGL
                     GANDKVASLEIHWPNGEVRTLQDIPANQILGIMQSLDTDEDSVRDVMDNCPAIANADQ
                     VDTDGDGQGDACDEDDDNDGIADRSDFAPLSAQPPTDSDGDGIADAWEMAHFGNLTSV
                     DATSDHDHDGITDVKEFAARGNPRVAALHDSLSLVPDQTQDGVAELAMVRMFEHYARL
                     DVVNGKTGEVLSTGRFYFNDYDPLGLVYMGRGSDSGMMEFAALAVDSYRNKAFIEIRT
                     ATGALVRSFELDSLPVTQMLQYESIAGQKRFVLPGYDAGRGQMVATLIDPAGRVERSV
                     DFGTGSTLVDFRTFQRQGEAWAVAGMQQAGGQFVLRLRSLVNDQQNQVVLPAGYALAA
                     IDLIEASTGHEGPYLVASQPRLVNRESEAGIRQFYLTSTFALAKTHTLPALRSLLAHG
                     VFTQPDPVFGFVNFIDAEGYAAQWVAKVQYGGGAYRFRYLPAQEIRIAYECQGTASIC
                     VASLRPDGRLIGFDAANQRSWVLNSKEQGTYQLTGN"
     gene            complement(5736..7574)
                     /locus_tag="PLQ18_00650"
     CDS             complement(5736..7574)
                     /locus_tag="PLQ18_00650"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020506120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HNE00184.1"
                     /translation="MAGAVAAALEDHGTLIAEAGTGTGKTFAYLVPALLSGKRVIVST
                     GTRALQDQLYRKDLPVICAALGSPAKLALLKGRSNYLCDYRLEAAEAAGRFRSREQAA
                     EFARIRHWAAMTRHGDIAEVIDVAEDSPVWPLVTSTADNCLGAECPRLADCKVLQARR
                     EALEADLLVVNHHLFFADMAIREEGFAELLPGADAVIFDEAHQLPEVASSFFGLTLSS
                     RQLVELARDTMIEQGRDARDFPDLAERAAQFEDRVTGLRQALGDEGRRAPWSEVGESA
                     PVIDAIAAITLALSRLLEALKEAAPRGKGLENCHARAVELAQRFAQLTQPPGDEAVHW
                     FETYARSFQIAQTPLDIAATFRGRMAMYQAAWVFTSATLAVGEGFGHYRHRLGLAESA
                     ATLKVDSPFDYARHAVLYHPEGLPEPAAPGYTAALIEAIVPMLEASRGRAFLLFTSYR
                     ALNEAHERLDGRVPYPLLKQGSLPKAALLDAFRRHGNAVLLGTQSFWEGVDVRGEALS
                     CVVIDKLPFSSPGDPVLAARIDAMKRAGGNPFFEYQIPNAVITLKQGVGRLIRDVDDC
                     GVLVLADPRLLTKGYGKLFLDSLPPMTRTRRLDRVQKFFAWAGRER"
     gene            complement(7680..8366)
                     /locus_tag="PLQ18_00655"
     CDS             complement(7680..8366)
                     /locus_tag="PLQ18_00655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE00185.1"
                     /translation="MTTLPRPPAPWRLAPLLALLLAAGCTAPSPFGAPVEDRTRAPAA
                     SRTRPLPPPAPGVEVMPLDAPAPLTPTEPLVGQPLPAAPAAPVAAPPPRPAPAPAPVA
                     SAPAAPSAPAEPEAERGGNNAVVALIDSANTYVRNNELDKAAASLERAQRLEPRNPNI
                     LYDLAQVRAHQAQYGQSEALAQKSIALAGGNKALQAKAWRLIAAVRRAGGNAAGGDAA
                     EAQAVTLGGR"
     gene            complement(8363..>8687)
                     /locus_tag="PLQ18_00660"
     CDS             complement(8363..>8687)
                     /locus_tag="PLQ18_00660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE00186.1"
                     /translation="KQLPLTPLDPAPPLEVATIAVDRASGGLADRNCHNTLMLPFLAG
                     SEPRALAPCHAGYVPPEGYAGGGMDTNGDTGSEEPVAGGSAEPRTAAPSNNDSIGDFF
                     RRLMQ"
BASE COUNT         1566 a         2867 c         2677 g         1577 t
ORIGIN      
        1 ctgcgccccg tcctgcagca gcaccccgcc gatggccacg atgacatcgc cttcgcgcag
       61 gccggcacgg gtcagcagat acgactcggg tcccagccgc aggcgcaggc catagccgtg
      121 atgatctgca agggagaccg gatcgaaacc ggcgagctgg cgcagggccg catccggctg
      181 gggggcaccc cgcaggcgcc tcgcgatcgg tcgcgcatcc accggcgtgc gttcctgccg
      241 gccggatgcg cgggcggacc cggcctgcgg gtcgtcctct gaaaactcgt gatcgtccgc
      301 cgctgccggg aacgccggca ccccggaaac tgccggggcc ggatcgcgcg gtgcgctcgt
      361 ccgcgccagg gcggattcgc ctgccggcat gggcacctcg atgctttcgc cgaagccggc
      421 gatctgcacc cggtccgcga acacggcggt caatcgccat ccgctggcca gggtcccgcc
      481 gaccggatag gcctgttcgg cctgtccgcc gaccgagatc aaggccagtc cgtgcccggc
      541 accggccagc gttccggtca gacgcagctg aggccgggcg gacggcttga gcgctgcagc
      601 gggtggcggc gtgtcccggg ggctttcggc cacgccgaac agcgcgtatc gacccagcgg
      661 ctcgggcacc ggctgcgtca cgacggggcc gcccgcatcg gtgccggaaa cctttgatgc
      721 gcccggcact tgcgccgtca cgagcgtggc catgtccacg gccatcagca tcgccaccag
      781 tccacaggcg atgtgtacgg aacgcccctg ccgggaagct cccggccgca agcgggtgat
      841 cccgaaactg taccgattcg cttcgtccat atccgtccgt tcccgtgcat cgatgccggg
      901 ccgcaggccg gaaccgcccg gtttcccgga cgctccgccg gtgcggccgg ctgcagcctt
      961 gcatccacca tccgtgcgca gtccccgccg ccggccgcaa accccggcac cgcatcaccg
     1021 gcgtcctgca ggcaggcgtc ctgcgcatcc cgcaagcccg cctgcctgcc gatctcccct
     1081 gcctttgcct gttacttcga ggcgctcagt tgccggtcag ctgatacgtg ccctgttcct
     1141 tgctgttcaa cacccatgag cgctggttgg ctgcatcgaa accgatcagc cggccatcag
     1201 gacgcaggga agccacacag atcgaagccg tgccctgaca ttcgtaggcg atcctgattt
     1261 cctgagccgg cagataccgg aagcggtatg caccgccgcc atactgcacc tttgccaccc
     1321 actgcgcggc atagccttcg gcatcaatga agttcacgaa accgaacacc gggtcaggct
     1381 gggtgaacac gccatgcgca agcagcgagc gcaaggccgg cagcgtgtgg gtcttggcca
     1441 gcgcgaaggt gctggtcaga taaaactgcc ggatacccgc ttcggattca cggttgacca
     1501 gccgcggctg cgaagccacc agataaggcc cttcatgccc ggtgctggct tcgatcagat
     1561 cgatggccgc aagcgcatag ccggccggca gcaccacctg gttctgctga tcattcacca
     1621 gactgcgcag gcgcagcacg aactgcccgc ccgcctgctg catgccggcc acggcccagg
     1681 cttctccctg acgctggaaa gtcctgaaat caaccagcgt gctgcccgtt ccgaaatcga
     1741 cgctgcgctc gacccgcccg gcaggatcga tgagggtcgc caccatctgt cccctgcccg
     1801 catcgtagcc cggcaggacg aaacgcttct gtccggcaat cgactcgtac tgcagcatct
     1861 gcgtcaccgg cagactgtcg agttcgaaac tcctgaccag tgcgccggtg gcagtcctga
     1921 tttcaatgaa ggccttgttt cgatacgaat ccacggccag cgcagcaaac tccatcatgc
     1981 cgctgtcact gccgcgcccc atgtacacca gccccagcgg gtcgtagtca ttgaaataga
     2041 agcgccccgt gctcaacact tccccggtct tgccgttgac cacgtccaga cgggcataat
     2101 gctcgaacat cctgaccatg gccagttcag ccacaccatc ctgagtctga tccggaacaa
     2161 gagacaggga atcgtgcagc gccgccaccc tcgggttgcc gcgggcagcg aattccttca
     2221 cgtccgtgat gccatcatga tcatgatcgg atgtcgcatc gaccgaggtc agatttccga
     2281 aatgagccat ttcccatgca tccgcgatac cgtcgccatc gctatcggtc ggtggctggg
     2341 cggaaagtgg cgcaaagtcg ctgcgatcag cgatgccatc attgtcatca tcctcatcac
     2401 aggcatcccc ctggccatcg ccatccgtat cgacctgatc ggcattggca atcgccgggc
     2461 agttatccat tacatccctg acgctgtcct catccgtatc cagggattgc atgatgccca
     2521 ggatctgatt ggcaggaatg tcctgcagag tccggacttc tccgttcggc cagtgtattt
     2581 ccagcgaagc aaccttgtca ttggcaccga gcccgaaatg cacacgccgc atgttctggc
     2641 cgtggttgtg cagttcctca ccgacttccc gcaactgttt gcgtccgtcg ggtgtcgtgg
     2701 caatgattct ggccccgatg gcatcacggt tggacggccc gccaaccaga ttcagctcca
     2761 gccagtggtt ggcattcccg gagttcctga acaattcgac cgggccctga ttgaaggggg
     2821 gttcaccgta gccgttggtg atgagcacat ccatgaatcc gtcgttgtca tagtcagcga
     2881 caatcaccgc ctcccccgcc ccttgcacgg acatgttcag accagcggcg tcaggggcgg
     2941 ccacgaaatg gccgctgcca tcattgagcc ataactgatt ggcgaaattc tttacgttgt
     3001 tggccttgag cagaaaaatg tccacatcca tgtcattatc gaaatcagcc actgccactg
     3061 ccattgtcgg tgtcgacgca tcgaatcccc agctggtcac gacatgccgg ccttgccgag
     3121 gatcgtacat gaacagcaaa tccgtcagct ttgtcgcctg ggtgcgcaga ctgctcatgt
     3181 cacgaatggc aatattcgtt gccctgatgt ttcctgccac cgcatagcca atgatccggt
     3241 cagtggccgg cgtgtggttc accagcgacc agagatgagt ggctggatcg aacccgacgt
     3301 agagtccggg cggcacattg ctgctgaact gcggcaggcc ctgattggcc gcatcattcg
     3361 gcgaaagcac gaaatgcttt tgagccggat tccggccggc cgaaccaatg aagatctgct
     3421 tgagattatg attaggagcc accagatcga aatcgacgct gccatctgca gaaaacacca
     3481 gtttctttgt ttcacccttc tgtggagaaa cagtaacctt gaacagtatg ttcccaagcg
     3541 gcttcacttc ggcataatcg ataccaagct gattatttct ggcaacatag aactcgctgt
     3601 tcaaatcatt gttgaaatcc gcgaccgccg catcgatcac cacgacactc ttctgctgcc
     3661 actcaaggaa ggctgcagag ttatccaccc acggcatgga ttctatgttg tatgcttttg
     3721 acggacttgc tccgcctcca tacagcagaa ccgttttccc ttggggaaac gcggcactct
     3781 gcgcaaatgc gatcgccccc ggaaacgcac cgcactggtc cgtaatatca tcgtacccat
     3841 gcgagttgct tttccacaca ctgggaaatg ccagaccatc cgaccggatg tccccggtct
     3901 gcatgatctc cagaattccg tcattatcga aatcatacgg gagcggcatt ctgccacgca
     3961 gatacggatt gctgaaattc atttccacgg cgcgatcttc aagcacgccg ccggcattga
     4021 ccagcagatt ggcatcatga ttcggactgg tggttgccac accgccggca gcgccggtca
     4081 gttccaccag atcctgatcg ccatcattgt cgatatccac ccataccgcg ccgtggttat
     4141 caccatcaaa attgtaattc ttcagcacct gcgaggcgac gttcgtgaac gtgccgtttt
     4201 tattgttgcg atacagatga gtcggcattc tgtgatttgt cagccagata tccagatagc
     4261 catcattatt gaaatccccc catgcgcccc cccagctttc accgatatat gcaatgccgg
     4321 ctgcttgcgt gacattcgtg aaactgactt ctgcagcccg cgacaagcag ggcacggaca
     4381 gcagcaggat cggcatcagc accatcaacc cgaaattatt gatgacccat ctgggtccgg
     4441 tcatgatgac ccgcacagcg gacttctcta cgagattcat tgcgtccatc ccctgtaact
     4501 ttcaaaaact gacttgcaca aaccatccag aattgctgca taactcccgg tccaggagtc
     4561 cgtgtcaaaa caaggcaggc ccatctcctg acgatgcaaa accatgacgg caatgatcag
     4621 ggctttcagg aagcggtgct caatcgccgg cagctgactg atccgattta ttcggcatga
     4681 cgaatccgtc cacgaacagt tcgctcacat gggctgatgg atggcagttt gccggtttta
     4741 atcgttaccc cttggaacgc ccctgcccat gggcattcaa ggtgagaacc ggactgcgat
     4801 ccgaatccat cccctgcacc tgatatcaga agcctgtcaa tgccctgccc cctcgtgcac
     4861 gtctggcatc gggaaccggg cggatgcctc cgtctttacc gtttcaggga actgcaaccc
     4921 ggctcccatc tctcatgaga caatagcgta cccctattac acccatgaga caaatttgtc
     4981 catagaagaa aagcttggtc atttttgtaa tacgaaaagc ctgtcgcccc gatatcttcc
     5041 ttcataaaag ttgatcgtga ctcaggcggg cttctggcgc catgatcaat cgcacatcac
     5101 cgcggttctt cgtcaggacg ccccttatca tttctgcatg atgagaagag gcatttaccc
     5161 aagcgcggaa cttggttttt gattctgctg ccgcagcagc cgtgcatgcg gcatgataaa
     5221 tccaaatcca acgtcatcac gacttaattc attgaacata aaggatattt aattttggtc
     5281 gatttgatgt ccggccagcc tcatccgata tggcggacat gggtcatgca gatagcggcc
     5341 aactcagtgc cacccgtgca aagccggatc atcggcgcaa aggcatgcgc aaattgcatg
     5401 ggttaatcgc cgtattccca aagttgaagc ggcgccatcc gggcttattc gccgggtcgc
     5461 tgttgtcttg cccatgcgaa gaacttatgc acccggtcga gccggcaccg acaggtcaat
     5521 ccccgccagg acggcgtggc gccggtcgag tgacaccgat ttcagtgccg aaggatcagg
     5581 gacgcggctg acagccccca gtcgccaccg ggtgccccgg ggtcgggcgc ctgcagatgg
     5641 ggcaggtctt cggcgcgcct ggagccgcct gcaggctgtc cggcccgcag gacttcctgc
     5701 ccggcgctcc gaagtccggt ccggaccgtt ttcagctatc gctcacgccc agcccacgca
     5761 aagaacttct gcacccggtc gagccggcgc gtgcgcgtca tcggcggcag gctgtcgagg
     5821 aacagcttgc cgtagccctt ggtcagcagc cgcggatcgg cgagcaccag cacgccgcag
     5881 tcgtcgacat cgcggatcag gcggccgacg ccctgcttga gcgtgatcac ggcgttcggg
     5941 atctggtact cgaagaacgg gttgccgccg gcgcgcttca tcgcgtcgat gcgcgcggcc
     6001 agcaccgggt cgccggggct ggagaacggc agcttgtcga tgacgacgca ggacagcgcc
     6061 tcgccgcgca cgtcgacgcc ttcccagaag ctctgcgtgc cgagcagcac ggcgttgccg
     6121 tggcggcgga aggcgtcgag cagtgcggcc ttcggcaggc tgccctgctt cagcagcgga
     6181 tacggcacgc ggccgtcgag ccgttcgtgg gcctcgttca gcgcgcggta gctcgtgaac
     6241 agcaggaagg cccggccgcg cgaggcttcg agcatcggca cgatcgcctc gatcagcgcc
     6301 gcggtgtagc cgggtgccgc gggttcgggc aggccctcgg ggtggtagag cacggcgtgg
     6361 cgggcgtagt cgaaggggct gtcgaccttg agtgtcgcgg cggattccgc gagaccgagc
     6421 cggtggcggt agtggccgaa gccttcgccg accgcgagcg tggccgacgt gaacacccag
     6481 gcggcctgat acatcgccat gcgcccgcgg aaggtcgcgg cgatgtcgag cggggtctgc
     6541 gcgatctgga agctgcgggc gtaagtctcg aaccagtgca cggcctcgtc gccgggcggc
     6601 tgcgtgagct gcgcgaagcg ttgcgcgagt tcgacggcac gcgcatggca gttctccagg
     6661 cccttgccgc gcggtgcggc ttccttcagg gcttcgagca ggcgtgacag cgccagcgtg
     6721 atggcggcga tggcgtcgat gaccggcgcg ctctcgccga cctcgctcca cggcgcgcgc
     6781 cggccctcgt cgccgagcgc ctgacgcagg ccggtgaccc ggtcctcgaa ctgcgcagcg
     6841 cgctcggcca gatccgggaa atcgcgggca tcgcgcccct gctcgatcat ggtgtcgcgc
     6901 gccagctcca cgagctggcg gctcgacagc gtcaggccga agaagctgct cgcgacctcg
     6961 gggagctgat gcgcctcgtc gaagatcacc gcgtcggccc cgggcagcag ctcggcgaag
     7021 ccttcctcgc ggatcgccat gtcggcgaag aacaggtggt ggttgaccac cagcagatcg
     7081 gcttcgagcg cctcgcgccg cgcctgcagc accttgcagt cggcaagccg cggacattcg
     7141 gcgccgaggc agttgtcggc cgtcgaggtc accagcggcc agaccggcga atcctcggcc
     7201 acgtcgatga cctcggcgat gtcgccgtgc ctcgtcatcg ccgcccagtg gcggatgcgg
     7261 gcaaactcgg cggcctgttc gcgcgaacgg aagcgtccgg cggcctcggc ggcctccaga
     7321 cggtaatcgc agaggtaatt ggagcggccc ttcagcagcg cgagcttcgc cggcgagccg
     7381 agcgcggcgc agatcaccgg cagatccttg cgatagagct ggtcctgcag cgcgcgcgtg
     7441 ccggtcgaga cgatcacgcg cttgccggac aaaagcgccg gcaccaggta cgcgaaggtc
     7501 ttgccggtgc cggtgccggc ctcggcgatc agcgtgccgt gatcctcaag cgcagcggcg
     7561 acggccccgg ccatcgcctg ctgctgcacg cgcggcgcgt agccgcggat cgcgcgcgcg
     7621 agcgggccat cggggccgaa gacggcatcg agttccagct tccggccccc ggctgcggct
     7681 cagcgaccgc cgagcgtgac ggcctgcgct tcggcggcat cgccgccggc ggcgttgccg
     7741 ccggcgcgac gcaccgcggc gatcagccgc caggccttgg cctgcagcgc cttgttgccg
     7801 ccagcgagtg cgatcgactt ctgcgccagc gcctccgact gcccgtactg cgcctggtgc
     7861 gcgcgcacct gcgcaaggtc gtacaggatg ttcggattgc gcggctccag ccgctgcgcg
     7921 cgttcgagcg aggcggcggc cttgtcgagt tcgttgttgc gcacgtaggt gttggccgaa
     7981 tcgatcagcg cgacgacggc gttgttgccg ccgcgttcgg cttcgggttc ggccggggcc
     8041 gacggtgcag ccggagcact ggcgaccggc gccggtgccg gcgccggacg gggcggcggc
     8101 gcagccaccg gtgcggccgg cgcagccggc agcggctggc cgaccagcgg ttcggtcgga
     8161 gtcagcgggg ccggggcatc gagcggcatg acctcgacgc ccggggccgg cggcggcagc
     8221 ggtcgtgtcc ggctggcagc cggggcgcgc gtgcggtcct cgaccggagc cccgaagggc
     8281 gagggagcgg tacagccggc tgccagcaac aaggccagca gcggagcgag gcgccagggc
     8341 gccggcggcc ggggcagcgt cgtcattgca tgagtctccg gaagaagtcg ccgatggaat
     8401 cgttgttgga cggggcggcg gtgcgcggtt cagcgctgcc acccgcgacg ggttcctcgc
     8461 tgccggtgtc gccgttcgtg tccatgccgc cgccagcata cccttcgggc ggcacgtatc
     8521 cggcatgaca cggcgccagc gcacgcggtt cggagccggc aaggaacggc agcatcaggg
     8581 tgttgtggca gttgcgatcg gccagtccgc cggaggcgcg atcgaccgcg atcgtcgcga
     8641 cctccagcgg cggtgccgga tcgagcggcg tcagcggcag ctgcttc
//
LOCUS       DAOQJH010000013         8529 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_4459_length_8529_cov_4.593226, whole genome shotgun sequence.
ACCESSION   DAOQJH010000013 DAOQJH010000000
VERSION     DAOQJH010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 8529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8529
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4459_length_8529_cov_4.593226"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..142)
                     /locus_tag="PLQ18_00665"
     CDS             complement(<1..142)
                     /locus_tag="PLQ18_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006398723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HNE00187.1"
                     /translation="MSPYTGRFAPSPSGPLHFGSLVTALASWLDARAAGGRWLLRIED
                     VDG"
     gene            272..751
                     /locus_tag="PLQ18_00670"
     CDS             272..751
                     /locus_tag="PLQ18_00670"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNE00188.1"
                     /translation="MTIQSLPLLHVIEPIPASDSALAALNLMLDLAINHLPVVDAHGH
                     YLGMLTLTDLLHEILPAGLRLGHGIGQPDVSFLGDAASLLSTHLGQLSARTAGDVADP
                     HVVPLETDCPLVEAARRLAEARNPLPVVDADRHIVGVLSVRLLLTHLLGRGVQNDAR"
     gene            741..2081
                     /locus_tag="PLQ18_00675"
     CDS             741..2081
                     /locus_tag="PLQ18_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsB/NhaD family transporter"
                     /protein_id="HNE00189.1"
                     /translation="MHGEPASVSHVIFGLPPLLVSLAIFVATYAIVISEKLNRAVIAA
                     LGAGLMILSGVLTQLEAVHGIDFNTIGLLTAMMVIVAITQKTGVFQYVAIVAAKRVKA
                     DPWGLLVMMAVVTAVFSAFLDNVTTVLLVTPVILLITEALGVPPYPYLFTVIFASNIG
                     GTATLIGDPPNIMIGSAANLSFNTFLLHLTPVVLVVFAATLLPIWLVWGRTLRATPEA
                     RAQVMAFDEKSAITDPRLLKQALAVIGAVIFGFMSAHPLHLEPATIAGFGAALLLTLQ
                     VVRHDAEHQTEEVAHALREIEWVTIFFFVGLFVVVTGIEKAGALKLMADWIVAATQGD
                     YKATAMAILWVSAVLSAVVDNIPFVATMIPVIQNMAPTFGADNLLPLWWALSLGACLG
                     GNGTLVGASANLVVAGFAERAGQPIRFKTFLLMAFPLMLMSIAISTVYLYLRYL"
     gene            complement(2151..3413)
                     /locus_tag="PLQ18_00680"
     CDS             complement(2151..3413)
                     /locus_tag="PLQ18_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009023402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNE00190.1"
                     /translation="MAIHEFFLMLAVLLLTARLFGELASRLGAPSVIGELLAGLVLGP
                     SVLGWVQPTETLMTLAEVGIILLLFEVGLDTNLFRLAEAGRKPFVVAAVGAFLPLVLG
                     FAAGWLLFDTSILAALFIGGTLTATSIGITVRVLDDLMRRRSDEAQIVIGAAVIDDIL
                     GVIILAFLYDFSSPKGVSLASTASIALFIVVFMVLAPIAGKIIAWAIDHTDRKLESEG
                     LLITLVISLIMLFSWLAHAVGAPAIMGGFAAGIALSQHFRMSITQRLRLPFRRTLERA
                     FAPRPELTHRLEKQFRPLLHTYTPIFFVMVGVSLDLSAVDWSSARVWLMAGVLVVLAV
                     AGKVASGWFIAEPTLRRAAIGLSMVPRGEVGLIFAKLGLGAAILSQELYAVLLIVVAL
                     STLLPPFALKAFYQRYGDRPELVMRDDD"
     gene            complement(3647..6022)
                     /gene="ppsA"
                     /locus_tag="PLQ18_00685"
     CDS             complement(3647..6022)
                     /gene="ppsA"
                     /locus_tag="PLQ18_00685"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019689470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HNE00191.1"
                     /translation="MAEYVVWFEALGMGDVDRVGGKNASLGEMISHLSAAGVQVPGGF
                     ATTAEAFREFLTVNGIIDRINAELAKLDIEDVNALAETGARVRQWVIDAPFQPALLDA
                     IETAYRDMTSRYGAELSVAVRSSATAEDLPDASFAGQQETFLNVRGLDQVMAAIKHVF
                     ASLYNDRAIAYRVHHGFEHGEVALSAGIQKMCRSDLGAAGVMFTLDTESGFDDAVFIT
                     ASYGLGETVVQGAVNPDEFYVHKPTLAAGRPAVLRRNLGGKAIKMVYNSAEQTVGRSV
                     KTVDVELAERRRFALADAEVEALARQALIIERHYGRPMDIEWGKDGTDGQLYILQARP
                     ETVKSRADSQVMQRYQLSRRGEVIATGRSIGQRIGSGVVRVISDIKEMAKVQAGDVLV
                     TDMTDPDWEPIMKRASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERVRDGEPVT
                     VSCAEGDTGYIYRGLIDFDIREVSLDAMPQLAFKIMMNVGNPDRAFDFSRLPNAGVGL
                     ARLEFIINRMIGVHPKALLNYDALPSDIKVRVDSQIAGYASPVDFYVDKLAEGIATLA
                     AAFAPKPVIVRMSDFKSNEYANLIGGPLYEPHEENPMIGYRGASRYVSESFRDCFELE
                     CRAMKKVRNDMGLDNVQVMIPFVRTLEEARQVVEILAANGLERGRDGLKLIMMCELPS
                     NALLADEFLEYFDGFSIGSNDLTQLSLGLDRDSGLIAHLFDERNPAVKKLLSMAIQAC
                     RRNGKYIGICGQGPSDHPDLAKWLMEEGIESVSLNPDTVVETWMFLAGEAA"
     gene            6268..6744
                     /locus_tag="PLQ18_00690"
     CDS             6268..6744
                     /locus_tag="PLQ18_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018871210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 domain-containing protein"
                     /protein_id="HNE00192.1"
                     /translation="MIFPVRAHAGLDPRPGSFSGLMDLYERNYMLLRRLLPAMLPADA
                     RQVSQAAGALDLHLHVSERFRYTSELSLTYRFEHDDGQVLLEPDLHVRIYHDARLAEV
                     MVARLRHAPPFRLGAAEEASELYARCRANRFLYKWLCYCTRQGHGFASATGAPHNS"
     gene            6834..7340
                     /locus_tag="PLQ18_00695"
     CDS             6834..7340
                     /locus_tag="PLQ18_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019626718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcription factor"
                     /protein_id="HNE00193.1"
                     /translation="MKLSTKGRYAVTAMMDLAIHDSHGPVTLADISQCQGISLSYLEQ
                     LFARLRRSGLVEGVRGPGGGYRLARRADAISVADIIAAVDERVDVTRCQGRGDCDNGR
                     QCLTHDLWNDLSERIATFLTGITLADFVDRPAVREMAERNALRQTQGHGHTPARRQLT
                     VRERRISA"
     gene            complement(7624..8058)
                     /locus_tag="PLQ18_00700"
     CDS             complement(7624..8058)
                     /locus_tag="PLQ18_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014996430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HNE00194.1"
                     /translation="MSDLHGYYIEDLSVGQSASYAKTVTEADVILFAGITGDDNPVHI
                     NQEFAETTMFKGRIVHGIFCGGLISTVAGTRLPGPGAIYIEQSLKFKAPVRIGDTVTA
                     TVTVTEINAAKRRVHADTVCTVHGKVVVEGKATFMVDNRPAG"
     gene            8303..>8529
                     /locus_tag="PLQ18_00705"
     CDS             8303..>8529
                     /locus_tag="PLQ18_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004272579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HNE00195.1"
                     /translation="MKVLVAVKRVVDYNVKVRPKADGTGVETANVKMSMNPFDEIAVE
                     EAVRLQEAGKASEVVVVSLGVSACQDTLRTAL"
BASE COUNT         1383 a         2788 c         2927 g         1431 t
ORIGIN      
        1 ggccgtcgac atcctcgatg cgcagcagcc agcgtccgcc ggcggcgcgg gcgtcgagcc
       61 agctcgcgag cgccgtgacc agcgagccga agtgcagcgg cccgctgggc gagggggcga
      121 aacggccggt gtaaggcgac atcgtgtgtt gtgtcatttc gtaaaggatt gcactggcgg
      181 atcatgccgg atgccggccg gaggatgccc aggcacctcg accgtgccat ccccttgatc
      241 ccagctgctg cgtttttgcg gagccagccc catgaccatc cagtcactgc ccctgctgca
      301 tgtgatcgaa ccgatcccgg ccagcgattc ggcgcttgcg gccctgaacc tgatgctcga
      361 tctggccatc aaccacctgc cggtggtcga tgcgcacggg cattacctcg gcatgctgac
      421 gctgaccgac ctgctgcacg agatcctgcc ggccgggctg cggctcggtc atggcatcgg
      481 ccagcccgac gtgagctttc tcggcgatgc cgcctcgctg ctgtcgacgc acctcggcca
      541 gctctcggca aggaccgccg gggacgtcgc cgatccgcac gtggtgccgc tcgaaaccga
      601 ctgcccgctg gtcgaggcgg cgcgccggct ggccgaggcg cgcaatccgc tgccggtcgt
      661 cgacgccgat cgccacatcg tcggcgtgct cagcgtgcgg ctgctgctga cccatctgct
      721 cggccgggga gtgcagaacg atgcacggtg aaccggcctc cgtctcccac gtgatcttcg
      781 ggctgccgcc gctgctggtg tcgctcgcga tcttcgtcgc cacctacgcg atcgtcattt
      841 ccgaaaagct caaccgcgcc gtgatcgccg cgctcggggc cgggctgatg atcctctccg
      901 gcgtgctgac gcagctcgaa gccgtgcacg gcatcgattt caacaccatc ggcctgctga
      961 cggcgatgat ggtcatcgtc gcgatcacgc agaagaccgg cgtgttccag tacgtcgcga
     1021 tcgtcgccgc caagcgcgtg aaggccgatc cctggggact gctggtgatg atggcggtgg
     1081 tcacggcggt gttctcggcc ttcctcgaca acgtcacgac cgtgctgctg gtgacgccgg
     1141 tgatcctgct gatcaccgag gcgctcggcg tgccgccgta tccgtatctg ttcacggtga
     1201 tcttcgcatc gaacatcggc ggcaccgcga cgctgatcgg tgacccgccg aacatcatga
     1261 tcggctcggc cgccaacctg agcttcaaca ccttcctgct gcatctgacg ccggtggtgc
     1321 tcgtcgtgtt cgcggcgacg ctgctgccga tctggctggt ctggggccgc acgctgcggg
     1381 cgacgccgga ggcgcgcgcc caggtcatgg ccttcgacga gaaatcggcg atcacggatc
     1441 cgcggctgct gaagcaggcg ctggcggtga tcggtgcggt gatcttcggc ttcatgagcg
     1501 cgcatccgct gcatctggag ccggcgacga tcgccggttt cggggctgcg ctgctgctga
     1561 cgctgcaggt cgtgcgccac gatgccgagc accagaccga ggaggtcgcg cacgcgctgc
     1621 gcgagatcga gtgggtgacg atcttcttct tcgtcggcct gttcgtcgtc gtcaccggca
     1681 tcgagaaggc cggcgcgctg aagctgatgg ccgactggat cgttgcggcc acgcaggggg
     1741 actacaaggc cacggcgatg gcgatcctgt gggtgtcggc cgtgctgtcg gccgtggtcg
     1801 acaacattcc gttcgtcgcg acgatgatcc cggtgatcca gaacatggcg ccgaccttcg
     1861 gggccgacaa cctgctgccg ctgtggtggg cgctgtcgct cggggcctgt ctcggcggca
     1921 atggcacgct ggtcggagcc agcgccaatc tggtggtcgc cggcttcgcc gagcgtgccg
     1981 gccagccgat ccgcttcaag accttcctgc tgatggcgtt tccgctgatg ctgatgagca
     2041 tcgcgatcag cacggtctat ctgtacctgc gctatctctg aacccgcatc cgcccggcac
     2101 ggctgacggt cgtgccgggc gggcggggcg atcggggggc ggtcaggaaa tcagtcgtcg
     2161 tcgcgcatca ccagttcggg ccggtcgccg taacgctggt agaaggcctt cagcgcgaac
     2221 ggtggcagca gcgtcgacag cgcgacgacg atcagcagca ccgcgtacag ctcctgactc
     2281 aggatcgccg ccccgagtcc gagcttcgcg aagatcagcc cgacctcgcc gcgcggcacc
     2341 atgctcaggc cgatcgcggc acggcgcagc gtcggttcgg cgatgaacca gccgctcgcc
     2401 accttgccgg cgactgccag cacgaccagc acgccggcca tcagccagac gcgcgccgag
     2461 gaccagtcga cggccgacag gtccagcgac acgccgacca tcacgaagaa gatcggcgtg
     2521 taggtgtgca gcagcggccg gaactgcttt tcgagccggt gcgtgagctc cggacgcggc
     2581 gcgaaagcgc gctccagcgt gcgccggaac ggcaggcgca ggcgctgcgt gatcgacatc
     2641 cggaagtgct ggctcagcgc gatgccggcc gcgaagccgc ccatgatcgc cggagcgccg
     2701 accgcgtggg cgagccacga gaacagcatg atcaggctga tgaccagcgt gatcagcagg
     2761 ccctcggatt cgagcttgcg gtcggtgtga tcgatcgccc aggcgatgat cttgccggcg
     2821 atcggcgcga gcaccatgaa cacgacgatg aacagcgcga tgctggcggt gctcgccagc
     2881 gacacaccct tcggcgacga gaagtcgtac aggaaggcga ggatgatgac gccgaggatg
     2941 tcgtcgatga ctgctgcgcc gatgacgatc tgcgcctcgt cgctgcgccg gcgcatcagg
     3001 tcgtcgagca cgcgcacggt gatgccgatg ctggtggcgg tcagcgtgcc gccgatgaac
     3061 agcgcggcca ggatcgaggt gtcgaacagc agccagcccg cggcaaatcc gagcacgagc
     3121 ggcaggaagg caccgacggc ggcgacgacg aagggcttgc ggccggcttc ggcgagccgg
     3181 aacaggttgg tgtcgaggcc gacctcgaac agcagcagga tgatgccgac ctcggcgagc
     3241 gtcatcagcg tctcggtcgg ctgcacccag ccgagcaccg aggggccgag caccaggccg
     3301 gcgagcagtt cgccgatcac ggatggggcg ccgaggcggc tggccagctc gccgaagagg
     3361 cgggcggtca gcagcagcac cgccagcatc aggaagaatt cgtggattgc cattgcttct
     3421 ccgggcctcg ttgcaggggg cggtgcgctg ccgcccgtag ggaggttggg cagtgtcgcg
     3481 gctcgaatcc ggcttggcaa ttcttacgcg taagaagaaa cggataccgt gttgattgtc
     3541 tcgctatctc gcgcaatcgc gccatgaaaa acggggtgcg gccgcgctgg cgaccgcacc
     3601 ccgtccgttc aggctgttca ggcggattca gcagtgaatc cgcagatcag gcggcttcgc
     3661 cggccaggaa catccaggtc tcgacaacgg tgtccgggtt cagcgacacg ctctcgatgc
     3721 cttcctccat cagccacttg gccagatccg ggtgatccga cggaccctgg ccgcagatgc
     3781 cgatgtactt gccgttgcgc cggcaggcct ggatcgccat cgacagcagc ttcttgaccg
     3841 ccggattgcg ctcgtcgaac aggtgcgcga tcaggccgga gtcgcgatcg agtccgagcg
     3901 acagctgggt caggtcgttc gagccgatcg agaagccgtc gaagtattcg aggaactcat
     3961 cggcgagcag cgcgttcgac ggcagctcgc acatcatgat cagcttcagg ccgtcccggc
     4021 cgcgttccag gccgttggcg gcgaggatct cgaccacctg acgcgcttcc tccagcgtgc
     4081 gcacgaacgg gatcatgacc tggacgttgt ccagacccat gtcgttgcgc accttcttca
     4141 tcgcccggca ttcgagctcg aagcagtcgc ggaagctctc cgagacgtac cgcgaggcgc
     4201 cgcggtagcc gatcatcggg ttttcctcgt gcggctcgta cagcgggccg ccgatcaggt
     4261 tcgcgtactc gttggacttg aagtccgaca tgcgcacgat gaccggcttc ggcgcgaagg
     4321 ccgcggccag cgtcgcgatg ccttcggcga gcttgtcgac gtagaagtcg accggcgagg
     4381 cgtagccggc gatctgcgag tcgacgcgga ccttgatgtc ggagggcagc gcgtcgtagt
     4441 tcagcagcgc cttcgggtgc acgccgatca tgcggttgat gatgaactcc agccgcgcca
     4501 gaccgacgcc ggcgttcggc agccgcgaga agtcgaaggc gcggtcgggg ttgccgacgt
     4561 tcatcatgat cttgaacgcg agctgcggca tcgcgtcgag gctgacctcg cggatgtcga
     4621 agtcgatcag gccgcggtag atgtagccgg tgtcgccctc ggcgcaggag accgtcaccg
     4681 gttcgccgtc gcgcacgcgc tcggtggcat cgccgcagcc gaccaccgcc gggatgccga
     4741 gctcgcgggc gatgatcgcc gcgtggcagg tgcggccgcc gcggttggtg acgatggcgg
     4801 aggcgcgctt catgatcggt tcccagtccg ggtcggtcat gtcggtcacg agcacgtcgc
     4861 cggcctggac cttggccatc tccttgatgt cgctgatcac gcgcacgacg cccgagccga
     4921 tgcgctggcc gatcgagcgg ccggtggcga tgacctcgcc gcggcgcgac agctggtagc
     4981 gctgcatgac ctgcgagtcg gcgcggctct tcacggtctc cgggcgggcc tgcaggatgt
     5041 agagctggcc gtcggtgccg tccttgcccc attcgatgtc catcggccgg ccgtagtggc
     5101 gctcgatgat cagcgcctga cgggcgagtg cctcgacctc ggcatcggca agcgcgaagc
     5161 ggcggcgctc ggcgagttcg acatcgacgg tcttgacgct gcgtccgacc gtctgctcgg
     5221 cgctgttgta gaccatcttg atggccttgc cgccgaggtt gcggcgcagc acggccgggc
     5281 ggccggcggc gagcgtcggc ttgtgcacgt agaactcgtc ggggttcacg gcgccctgca
     5341 cgacggtctc gccgaggccg taggaggcgg tgatgaacac cgcgtcgtcg aagccggatt
     5401 cggtgtcgag cgtgaacatg acgccggcgg caccgaggtc ggagcggcac atcttctgga
     5461 tgccggccga cagcgcgacc tcgccgtgct cgaagccgtg gtgcacgcgg taggcgatgg
     5521 cgcggtcgtt atataaggag gcgaacacgt gcttgatcgc agccatgacc tgatcgaggc
     5581 cgcgcacgtt caggaaggtt tcctgctggc ccgcgaacga ggcatcgggc agatcctcgg
     5641 cggtcgccga ggagcgcacc gcgaccgaca gctcggcacc gtagcgcgat gtcatgtcgc
     5701 ggtacgcggt ttcgatcgcg tcgagcagcg ccggctggaa cggcgcatcg atgacccact
     5761 ggcgcacgcg cgcaccggtc tcggccagcg cgttcacatc ctcgatgtcg agcttcgcca
     5821 gttcggcgtt gatgcggtcg atgatgccgt tgacggtcag gaactcgcgg aaggcctcgg
     5881 ccgtggtcgc gaacccgccc ggtacctgga cgccggcagc agacagatgg ctgatcatct
     5941 cgccgagcga cgcgttcttg ccgccgacgc ggtccacgtc gcccatgccg agggcttcaa
     6001 accaaacgac gtactctgcc aatgtagttt cctcttcgga acgcatttag aagcctggga
     6061 aagccggctg cgcaggtacc gggaccgccg tgccgatgcg tacggcgctg tcctggtgac
     6121 ccggcacgaa agcccgggcc gcgcgaggcg cgcgattata atgtcgcccc cacagggacg
     6181 gaacggattt cggcatccat ctcggggatg ccggcgcggc tgtatccagt cctcacaaaa
     6241 gcacggcggg accacgcgtt ggtgaatctg atctttccgg tccgggccca cgccggcctc
     6301 gatccgcgtc ccggcagctt ctcgggcctg atggacctct acgagcgcaa ctacatgctg
     6361 ctgcgccggc tgctgccggc catgctgccg gcagatgcga gacaggtctc gcaggcagcc
     6421 ggggcgctcg acctgcacct gcacgtcagc gagcgcttcc gctacaccag cgagctgtcc
     6481 ctgacctacc gcttcgagca cgacgacggc caggtgctgc tggagccgga cctgcacgtg
     6541 cgcatctatc acgatgcgcg gctcgccgag gtcatggtcg cgcggctgcg tcacgcaccg
     6601 ccgttccggc tcggggccgc cgaggaggcg agcgagctgt acgcgcgctg ccgggccaac
     6661 cgtttcctgt acaaatggct gtgttactgc acgcgccagg ggcacggttt cgcgtccgcc
     6721 accggtgcac cgcacaatag ttgatcgaat cgcttgggta ccgtagcatc gaaaccagac
     6781 tgcgccgaag gtcacccggg ccggcggtgc gcaagacggc tgcggaggta ctcatgaagc
     6841 tttcgacgaa agggcgctac gcggtgacgg cgatgatgga tctggcgatc catgattcac
     6901 acggaccggt gacgctggcg gacatctcgc agtgccaggg tatctcgctg tcctatctgg
     6961 agcagctgtt cgcgcgtctg cgtcgctcgg gactggtcga gggcgtgcgc ggacccggtg
     7021 gtggttaccg gctggcgcgg cgtgccgatg cgatcagcgt tgccgacatc atcgccgccg
     7081 tcgacgagcg cgtggatgtg acccgctgcc aggggcgcgg cgactgcgac aacggccgcc
     7141 agtgcctgac gcacgatctc tggaatgacc tgagcgagcg catcgcgacc ttcctgaccg
     7201 gcatcacgct cgccgatttc gtcgaccgcc cggcggtgcg cgagatggcc gagcgcaacg
     7261 cgctgcgcca gacgcagggc cacggccaca cgccggcccg gcgccagctg acggtgcggg
     7321 agcgccgcat cagcgcctga agccgcaagt tttgcctccg cgttcacgac gcccccgtct
     7381 gcgacggggg cgtcgtgctg tccgggcata aaaaaagcgg ggaccgaagt ccccgctcaa
     7441 gataaacctg cctcaggaga tcaaacgagc ttgtcgcccg gagccgcccg cgagaacggc
     7501 tccgccgtga agctcaaggt cacacaccgc cattcctcaa tgcgaccttg aggcgtattg
     7561 aacgcccggg ctcgcgcagg cgcgttgatc tgcatcaagc cggcgtgtga cggcagcgcg
     7621 ggctcagccc gccgggcggt tgtcgaccat gaaggtcgcc ttgccctcga cgaccacctt
     7681 gccgtgcacc gtgcagacgg tgtcggcgtg cacgcggcgc ttggcggcgt tgatctcggt
     7741 gacggtgacg gtcgcggtga ccgtgtcgcc gatgcgcacc ggcgccttga acttcagcga
     7801 ctgctcgatg tagatcgcgc ccggcccggg cagccgcgtg ccggcgaccg tcgagatcag
     7861 cccgccgcag aagatgccgt gcacgatgcg acccttgaac atcgtcgttt cggcaaattc
     7921 ctggttgatg tgcaccgggt tgtcgtcgcc ggtgatgccg gcgaacagga tcacgtcggc
     7981 ttcggtgacg gtcttcgcgt aggacgcgct ctggccgacg ctcaggtctt cgatgtagta
     8041 gccgtgcaga tcggacatgg tcaagcagcc tcgggggtgg ggagggagag ggcaggacag
     8101 ggtaagaaaa cccgggcccg gcgcatagac tgtgccggct cgccccggac gatcccggca
     8161 tcttgcgacg gcactgggta atcggtaaag tcgtactagg gttttccctt agtgctcatt
     8221 ccgataatac cggtatccag cgtcgccgat cgcggccggg ccggacggga agccgcatat
     8281 atcccaccga ggtaccgcat cgatgaaagt cctggtcgcc gtcaaacggg tggtcgatta
     8341 caacgtcaag gtgcgtccga aagcggacgg caccggcgtc gagactgcca acgtcaagat
     8401 gtcgatgaat cccttcgacg agatcgccgt cgaggaagcc gtgcgcctgc aggaggccgg
     8461 caaggccagc gaggtcgtcg tcgtctcgct cggcgtttcc gcctgtcagg acacgctgcg
     8521 caccgccct
//
LOCUS       DAOQJH010000014         8285 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_4667_length_8285_cov_3.956258, whole genome shotgun sequence.
ACCESSION   DAOQJH010000014 DAOQJH010000000
VERSION     DAOQJH010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 8285)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8285)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8285
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4667_length_8285_cov_3.956258"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..669)
                     /gene="ispH"
                     /locus_tag="PLQ18_00710"
     CDS             complement(<1..669)
                     /gene="ispH"
                     /locus_tag="PLQ18_00710"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HNE00196.1"
                     /translation="MQIRLANPRGFCAGVDRAIEIVERALDLFGAPIYVRHEIVHNRY
                     ICDKLRAEGAIFIEDLAEVPDGSTLIFSAHGVSRAIHDEAARRGLKVFDATCPLVTKV
                     HMEVVRYAKQGIGVVLIGHAGHPEVEGTMGHYDRSAGGAIHLVETVADVARLTVADPQ
                     RLAFVTQTTLSVDDTVGIIDALRARFPAIHGPRKDDICYATQNRQDAVKELAADCDVV
                     LVVGS"
     gene            complement(682..1155)
                     /gene="lspA"
                     /locus_tag="PLQ18_00715"
     CDS             complement(682..1155)
                     /gene="lspA"
                     /locus_tag="PLQ18_00715"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013031973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HNE00197.1"
                     /translation="MARWLWLSVAVLALDQISKYAAETLLLLHRPVPVLPPVLDFMLA
                     YNTGAAFSFLAGAGGWQRWFFIVLAGAVSVALTVWIVRLHAHERRLAVALALVLGGAI
                     GNLVDRALWGHVIDFILVHFGDWPFPAFNLADSAITVGAALLVLDSLFPHRAPDA"
     gene            complement(1155..3998)
                     /gene="ileS"
                     /locus_tag="PLQ18_00720"
     CDS             complement(1155..3998)
                     /gene="ileS"
                     /locus_tag="PLQ18_00720"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007193116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HNE00198.1"
                     /translation="MADYKDTLNLPDTAFPMKGNLPQREPEQLARWAGMNLYAQQRLA
                     CAGRPKFVLHDGPPYANGDIHIGHAVNKVLKDIIVKSRTLAGFDAPYVPGWDCHGLPI
                     EHAVEKKVGKVGVKVDARSFRQQCRAFAHGQVDRQREDFIRLGVLGDWQHPYLTMDFR
                     TEADIVRSLGRIVGNGHLLRGSKPVYWCLDCGSALAEAEVEYEDKDSLAIDVRFPVVE
                     AASVFARLHHAEGGDGKGPLSVVIWTTTPWTLPANRAVTLGAELEYVVVQAETAQGSE
                     RLLLAEGLLKDCMLRWGVERYQVLAYGKGADLEGLKLAHPFYEREVPVILGEHVTLEA
                     GTGCVHTAPAHGQDDYVVSSRYGLRIDDNPVGPDGRFLPETPLFGGLHVHGANERILD
                     VLKGHARLVHAEKLRHSYPHCWRHKTPIIFRATPQWFISMEQAGLRAQALAAVEQLRF
                     TPDWGRARLEGMIANRPDWCISRQRYWGVPIVLFTHRRSGELHPRWAELIEQVALKIE
                     QEGIDGWFSSTAEDWLGPEAADYEKVTDTLDVWYDSGTTHASVLERRPELGFPADLYL
                     EGSDQHRGWFQSSLLASVAMRGVAPYKGLLTHGFTVDQQGRKMSKSLGNVIAPQEVIK
                     ELGADVLRLWVAATDYRGEMTVSKEILRRMADSYRRIRNTARFLLANLNGFDPARDAV
                     EPARMLPLDRWVVDRAARLQAEVCSAYDDYLFHVIYQKVHNFCSVDLGSFYLDVIKDR
                     QYTGARDGLPRRSAQTALYHVLEALVRWIAPICSFTAEEIWKHMPGARAESIFLDTTW
                     YPGLFTLDADDAFGADYWARLIDVRGAVSKAIEDLRKGGGIGGSLEAEVDLYADESLR
                     TDLARLHDELRFVLITSQARVHPLDQAPGSAVLAPDGRLALVVARSEAQKCVRCWHLR
                     PDVGTHAAHPQLCGRCIENVDQAGAGGERRLYA"
     gene            complement(4038..4976)
                     /gene="ribF"
                     /locus_tag="PLQ18_00725"
     CDS             complement(4038..4976)
                     /gene="ribF"
                     /locus_tag="PLQ18_00725"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020507214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HNE00199.1"
                     /translation="MELIRGTHNLRPRHRGCVATIGNFDGVHRGHHAVLDQLHARADA
                     LGLPAVVITFEPYPQEYFARSQPPARLTRLREKLVALEAAGVQRVLVLAFNARLAAMR
                     AGDFVQHLLVDGLGVRFLVVGDDFRFGHGREGDYALLERCGAAAGFEVAHTATHLIDG
                     VRVSSTRIRERLAAGELAEAARLLGRPYAVHGRVAHGQQLGRTIGFPTANVHLRRRAT
                     PLGGVYAVVLRSTGLPPLAGVANLGRRPTVGGTREQLEVHCFDFDGDLYRRRVQVEFR
                     ERLRPEQRFPSLDELKLQIARDAEAARAHFRARPAD"
     gene            complement(5073..6614)
                     /gene="murJ"
                     /locus_tag="PLQ18_00730"
     CDS             complement(5073..6614)
                     /gene="murJ"
                     /locus_tag="PLQ18_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014705543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HNE00200.1"
                     /translation="MSSKSLMRSTGVVSALTFLSRLLGFVRDMVYAGLFGAGAGMDAF
                     NVAFRIPNLFRRLVGEGAFSQAFVPVFSQYRTQRSPEELRLLVDQVAGTLGTVLLALT
                     AFGMIGAPLLVWAFASGYGDEPYKFALTEQMLRITFPYVLCICLTAFAGGILNSCGHF
                     AVPAVTPVLLNVTMIAAALLAAPHFAEPALALAWGVALAGVLQLLFQWPALKRIGLVP
                     RPRLAWRAEGVRRIMMLMVPALIGSSAAQINLLINTQLASTLPTGSVSWLYYSDRLVE
                     FTLGIFGAALGTVILPKLSRQHAAAEGEGFAHTLDWGLRWSLLIGTPATVALAILAGP
                     LLATLFMRGEFTATDVEMTTRSLMTYGSGLVAYMLVKVLAPGFYARQDTKTPVRFGLI
                     SIGANIGFQMLLIAPLAHAGLALSTALAAFVNVALLYWQLRRDGVYRPRPGWPAFWLR
                     MLAANAAMAGFLVWAAGDLSGWIAADTLTRVLRLAWIVPAGAGVYAAVALGLGVRLSQ
                     LRAPA"
     gene            6837..7100
                     /gene="rpsT"
                     /locus_tag="PLQ18_00735"
     CDS             6837..7100
                     /gene="rpsT"
                     /locus_tag="PLQ18_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020507209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HNE00201.1"
                     /translation="MANTAQAKKRVRQAEKARQHNASLRSRFRTYVKKVIAAIRAGDK
                     SQAEAAFRSATSVIDSTARKGLIHVNKAARHKSRLSAHIKAMA"
     gene            complement(7239..>8285)
                     /gene="proB"
                     /locus_tag="PLQ18_00740"
     CDS             complement(7239..>8285)
                     /gene="proB"
                     /locus_tag="PLQ18_00740"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017939098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HNE00202.1"
                     /translation="RGLDAAAIEAWVAQLAELHAEGRDVIIVSSGAVAEGMRRLGWRA
                     RPKALAELQAAAAVGQMGLVQGWEAAFQKHGIRTAQILLTHEDVSDRQRYLNARNTLR
                     TLTRLRVVPVINENDTVAFEEIRFGDNDTLGALVANLVEAELYVILTDQHGMYDRDPR
                     AHADARFLSEVSAGDPALERRAGGGAGTLGRGGMLTKVRAAAKAARSGTATLLAWGRE
                     PEVLRRVARGEPIGTLLRPQAGPVAARKQWLAVQLQVRGWLHLDAGAVQALKLGGRSL
                     LAVGVRGVEGEFQRGELVACVGPDGVEVARGLVNYDASESVRIMGKPTEAIESVLGYV
                     DAPALIHRDDLVLV"
BASE COUNT         1312 a         2923 c         2918 g         1132 t
ORIGIN      
        1 cgaaccgacc accagcacca cgtcgcagtc ggcggcgagt tccttgaccg cgtcctggcg
       61 gttctgcgtc gcgtagcaga tgtcgtcctt gcgcgggccg tggatggccg ggaagcgcgc
      121 gcgcagcgca tcgatgatgc cgaccgtgtc gtcgaccgac agcgtggtct gcgtgacgaa
      181 ggccaggcgc tgcggatcgg caaccgtgag gcgcgccacg tcggcgacgg tctcgaccag
      241 atggatggcg ccgccggccg accggtcgta atggcccatc gtgccctcga cctcggggtg
      301 accggcgtgg ccgatcagca cgacgccgat gccctgcttg gcgtagcgca cgacctccat
      361 gtgcaccttg gtgaccagcg ggcaggtggc gtcgaacacc ttgaggccgc ggcgggcggc
      421 ctcgtcgtgg atcgcgcgcg agacgccgtg cgccgagaag atcagcgtcg agccgtccgg
      481 aacctcggcc agatcctcga tgaagatcgc gccttcggcc cgcagcttgt cgcagatgta
      541 gcggttgtgg acgatctcgt gacgcacgta gatcggcgcg ccgaacaggt cgagcgcgcg
      601 ctcgacgatc tcgatggcgc ggtcgacgcc ggcacagaaa ccacgcggat tggcgaggcg
      661 gatctgcatg gcggggactc ctcaggcgtc gggcgcccgg tgcgggaaca ggctgtcgag
      721 cacgagcagg gccgcgccga cggtgatcgc cgaatcggcg aggttgaagg ccgggaacgg
      781 ccagtcgccg aaatgcacga ggatgaagtc gatgacgtgg ccccacagcg cgcggtcgac
      841 gaggttgccg atcgcaccgc cgagcaccag cgccagcgcc acggccagcc gccgctcatg
      901 cgcatgcagg cgcacgatcc agaccgtcag cgccaccgat accgcgccgg ccagcacgat
      961 gaagaaccag cgctgccagc cgccggctcc ggcgaggaag ctgaacgccg cgccggtgtt
     1021 gtaggcgagc atgaagtcga gcaccggcgg cagcaccggc accggccggt gcagcagcag
     1081 cagggtctcg gcggcgtact tgctgatctg atcgagcgcg agcacggcca cgctcagcca
     1141 aagccagcgc gccatcaggc gtacagccgg cgttcgccgc ctgcgccagc ctgatcgacg
     1201 ttctcgatgc agcgcccgca gagctgcggg tgcgcggcat gcgtgccgac atcggggcgc
     1261 aggtgccagc agcgcacgca cttctgcgcc tcgctgcgcg cgacgaccag cgccagccgg
     1321 ccgtccgggg cgagcacggc ggagccgggt gcctgatcga gcggatgcac gcgcgcctgc
     1381 gaggtgatca gcacgaagcg cagttcgtcg tgcaggcggg cgagatcggt gcgcaggctt
     1441 tcgtcggcgt acaggtcgac ctcggcctcc agcgagccgc cgatgccgcc gcccttgcgc
     1501 aggtcctcga tcgccttgct gaccgcgccg cgcacgtcga tcagccgcgc ccagtagtcg
     1561 gcaccgaagg catcgtcggc atcgagcgtg aacaggcccg ggtaccaggt ggtgtcgagg
     1621 aaaatgctct cggcgcgcgc accgggcatg tgcttccaga tttcctcggc cgtgaacgaa
     1681 cagatcggcg cgatccagcg caccagggct tcgagcacgt ggtacagcgc ggtctgcgcc
     1741 gagcgccgcg gcaggccgtc gcgggcgccg gtgtactgac ggtccttgat cacgtcgagg
     1801 tagaagctgc cgaggtccac cgagcagaag ttgtggacct tctggtagat gacgtggaac
     1861 aggtagtcgt cataggccga gcagacctcg gcctgcagcc gggcggcgcg gtcgacgacc
     1921 cagcgatcga gcggcagcat gcgcgccggc tcgacggcat cgcgcgccgg atcgaagccg
     1981 ttgaggttgg cgagcaggaa gcgcgcggtg ttgcggatgc gccggtacga atcggccatg
     2041 cgccgcagga tttccttcga gaccgtcatc tcgccgcggt agtcggtggc ggcgacccag
     2101 agcctgagca cgtcggcgcc gagttccttg atgacctcct gcggggcgat gacgttgccg
     2161 agcgacttcg acatcttgcg gccctgctgg tcgaccgtga agccgtgcgt cagcagaccc
     2221 ttgtacggcg ccacgccgcg catcgccacc gaggcgagca gcgaggactg gaaccagccg
     2281 cggtgctggt ccgagccttc gaggtacaga tcggccggga agccgagttc ggggcggcgc
     2341 tccagcaccg aggcgtgcgt cgtgccggag tcgtaccaga catcgagcgt gtcggtgacc
     2401 ttctcgtagt cggcggcctc gggaccgagc cagtcctcgg ccgtgctcga aaaccagccg
     2461 tcgatgccct cctgctcgat cttcagtgcc acctgctcga tcagttcggc ccagcgcgga
     2521 tgcagctcgc cgctgcgccg gtgcgtgaac agcacgatcg gcacgcccca gtagcgctgg
     2581 cgcgagatgc accagtccgg gcggttggcg atcatgcctt cgaggcgcgc ccggccccag
     2641 tcgggcgtga agcgcagctg ttcgacggcg gcgagcgcct gcgcgcgcag gccggcctgc
     2701 tccatcgaga tgaaccactg cggcgtggcg cggaagatga tcggcgtctt gtgccgccag
     2761 cagtgcgggt agctgtggcg cagcttttcg gcatgcacga gccgggcgtg gcccttgagc
     2821 acgtcgagga tgcgctcgtt ggcgccgtgc acgtgcaggc cgccgaacag cggggtttcg
     2881 ggcaggaagc ggccgtccgg gccgaccggg ttgtcgtcga tgcgcaggcc gtagcggctg
     2941 ctgacgacgt agtcgtcctg gccgtgcgcc ggcgccgtgt gcacgcagcc ggtaccggct
     3001 tcgagcgtga cgtgctcgcc gaggatcacc ggcacctcgc gctcatagaa cgggtgcgcg
     3061 agcttcagcc cttcgaggtc cgcgcccttg ccgtaggcga gcacctgata gcgctcgacg
     3121 ccccagcgca gcatgcagtc cttgagcagg ccctcggcga gcagcaggcg ttcgctgccc
     3181 tgcgcggtct cggcctgcac caccacgtat tcgagttcgg ccccgagcgt cacggcgcgg
     3241 ttggccggca gcgtccacgg cgtcgtcgtc cagatcacga ccgacagcgg ccccttgccg
     3301 tcaccgccct cggcgtggtg caggcgcgcg aacacgctcg cggcctcgac caccgggaag
     3361 cgcacgtcga tggccagcga atccttgtcc tcgtactcga cctcggcctc ggcgagcgcc
     3421 gagccgcagt cgaggcacca gtacaccggc ttcgagccgc gcagcaggtg gccgttgccg
     3481 acgatgcgcc cgagcgaacg cacgatgtcg gcctcggtgc ggaagtccat ggtcagatac
     3541 gggtgctgcc agtcgccgag cacgccgagg cggatgaagt cctcgcgctg gcgatcgacc
     3601 tggccgtgcg cgaaggcgcg gcactgctgg cggaagctgc gggcatcgac cttgacgccg
     3661 accttgccga ccttcttctc gacggcgtgc tcgatcggca ggccgtggca gtcccagccc
     3721 ggcacgtagg gggcatcgaa gccggcgagc gtgcgcgact tgacgatgat gtccttcagc
     3781 accttgttga cggcgtggcc gatgtggatg tcgccgttcg catacggcgg accgtcgtgc
     3841 agcacgaact tcggccggcc ggcgcaggcg aggcgctgct gcgcgtagag gttcatccct
     3901 gcccagcgcg caagctgctc gggctcgcgc tgcggcaggt tgcccttcat cgggaaggcc
     3961 gtgtcgggca ggttcagggt gtccttgtaa tcggccatgc tcacctcgcg ccgcttgcgg
     4021 cgccatgcag tcggagttca gtccgccggc cgggcgcgga aatgcgcacg cgcggcctcg
     4081 gcgtcccggg caatctggag cttcagttcg tccagcgacg gaaagcgctg ttcggggcgc
     4141 agccgctcgc ggaactcgac ctgcacgcgc cgccggtaca ggtcgccgtc gaaatcgaag
     4201 cagtggactt cgagctgctc gcgcgtgccg ccgaccgtcg gccggcggcc gaggttggcg
     4261 accccggcga gcggcggcag gccggtgctg cgcagcacca cggcgtagac cccgccgagc
     4321 ggcgtcgcgc gccggcgcag gtgtacgttg gcggtcggaa aaccgatggt gcggccgagc
     4381 tgctggccat gcgcgacgcg cccgtgcacc gcgtacggcc gcccgagcag gcgcgcggcc
     4441 tcggcgagct caccggccgc cagcctctca cggatgcgcg tgctgctgac gcgcacgccg
     4501 tcgatcaggt gcgtggcggt gtgcgcgacc tcgaaaccgg ccgccgcacc gcagcgttcg
     4561 agcagcgcgt agtcgccctc gcggccgtgg ccgaagcgga agtcatcgcc gaccacgagg
     4621 aagcgcacgc cgagaccgtc gaccagcagg tgctgcacga aatcgccggc gcgcatcgcc
     4681 gcgagccggg cattgaaggc cagcaccagc acgcgctgca cgccggcggc ttcgagcgcg
     4741 accagcttct cgcgcagccg cgtcagccgc gccggcggct ggctgcgcgc gaagtattcc
     4801 tgcgggtagg gctcgaaggt gatcaccacc gccggcaggc cgagcgcatc ggcacgcgcg
     4861 tgcagctggt cgagcaccgc gtggtggccg cggtgcacgc cgtcgaaatt gccgatggtc
     4921 gcgacgcagc cgcggtgtcg ggggcgcaga ttgtgcgtgc cgcgtatcag ttccatgccg
     4981 ggtgccgggc tgctccgggc gcgtcgcgcc gctgaaaaag caagcgagcg attataagga
     5041 agcgcccggg cttgtcatgc cggcccgcgc catcaggccg gcgcgcgcag ctgcgacagc
     5101 cgcacgccga gcccgagcgc caccgccgcg tagacgccgg ccccggccgg cacgatccac
     5161 gcgagccgga gcacgcgcgt cagcgtgtcg gcggcgatcc agcccgagag atcgccggcc
     5221 gcccagacca gaaagcccgc catcgccgcg ttggccgcga gcatgcgcag ccagaacgcc
     5281 ggccagccgg gccgcggccg gtagacgccg tcgcggcgca gctgccagta cagcagcgcg
     5341 acgttgacga aggcggcaag tgccgtcgac agcgcaaggc ctgcgtgcgc gagcggcgcg
     5401 atcagcagca tctggaagcc gatgttggcg ccgatcgaga tcaggccgaa acgcaccggg
     5461 gtcttcgtgt cctgacgcgc gtagaagccg ggcgccagca ccttgaccag catgtaggcg
     5521 accaggccgc tgccgtaggt catcaggctg cgcgtggtca tctcgacatc ggtcgccgtg
     5581 aactcgccgc gcatgaacaa ggtggcgagc agcggaccgg cgaggatcgc gagcgcgacg
     5641 gtcgcggggg tgccgatcag cagcgaccag cgcagccccc agtcgagggt gtgcgcgaag
     5701 ccctcgcctt cggccgcggc gtgctggcgc gacagcttcg gcaggatcac ggtgccgagc
     5761 gccgcaccga agatgccgag cgtgaactcg accagccggt ccgagtagta cagccacgac
     5821 acgctgccgg tcggcagcgt cgaggcgagc tgggtgttga tcagcaggtt gatctgcgcg
     5881 gcactcgacc cgatcagcgc cggcaccatc agcatcatga tccggcgcac gccctcggca
     5941 cgccaggcca gccgcgggcg cggcaccagc ccgatgcgct tcagcgccgg ccactggaac
     6001 agcagctgca gcacgccggc aagcgccacg ccccaggcga gcgccagcgc cggctcggcg
     6061 aagtgcggcg ccgccagcag cgccgcggcg atcatcgtca cgttcagcag caccggcgtc
     6121 accgccggca ccgcgaagtg cccgcagctg ttgaggatgc cgccggcgaa cgcggtcagg
     6181 cagatgcaga gcacgtacgg gaacgtgatg cgcagcatct gctcggtcag cgcgaacttg
     6241 tacggctcat cgccgtagcc gctcgcgaac gcccagacca gcagcggcgc gccgatcatg
     6301 ccgaaggcgg tcagcgcgag cagcacggtc ccgagcgtgc cggccacctg atcgaccagc
     6361 aggcgcagct cctcggggct gcgctgcgtg cggtactgcg agaacaccgg cacgaaggcc
     6421 tgcgagaagg cgccctcgcc gaccagccgc cggaacaggt tcggaatgcg gaaggcgacg
     6481 ttgaaggcat ccatgccggc accggcgccg aacaggccgg cgtagaccat gtcgcgcacg
     6541 aagccgagca ggcgcgacag gaaggtcagc gcgctgacca cgccggtgga ccgcatcagg
     6601 gacttgctgc tcatgtaagg atttcttttc aggatttctt tttccgggcc ccggcccgcg
     6661 ggagcgcgga tcataagcag cgtgcgcagc cctcgccagc gcgcctgcag cattgacagc
     6721 ggcccgccgg acgcggacaa ttcgcgcctt ttccagcgtc ggccgccact gcccgggcgt
     6781 ccgcagcctc gcagcactgc atcgactgca tcacacgaat tccaggagcc ccgacagtgg
     6841 ccaatactgc ccaagccaag aagcgcgtcc gccaggcgga gaaggcccgc cagcacaacg
     6901 ccagcctgcg ttcgcgcttc cgtacctacg tcaagaaggt catcgccgcg atccgtgccg
     6961 gtgacaagtc gcaggccgaa gccgcgttcc gctcggcgac ctccgtgatc gacagcaccg
     7021 cccgcaaggg cctgatccac gtgaacaagg ccgcccgcca caagagccgc ctgtccgcgc
     7081 acatcaaggc gatggcgtaa gcacacgcct gccgccacac cgaaaaagcc ggggcgaccc
     7141 ggcttttttc atgcctcgat tttcatgcct cgattccggc ggaccggatc gcccgccgcc
     7201 ctgcggcagc gggcagtgcc ggcgtcagac catctgcgtc agaccagcac cagatcgtcg
     7261 cgatggatca gtgccggcgc atcgacgtag ccgagcaccg attcgatcgc ctcggtcggc
     7321 ttgcccatga tgcgcaccga ttccgacgcg tcgtaattga ccaggccgcg cgcgacctcg
     7381 acgccatccg ggccgacgca ggcaaccagc tcgccgcgct ggaactcgcc ctcgacgccg
     7441 cgcacgccga ccgcgagcag cgaacgtccg ccgagcttca gcgcctgcac ggcgccggca
     7501 tcgaggtgca gccagccgcg cacctgcagc tgcacggcca gccactgctt gcgcgccgcc
     7561 accggcccgg cctgcggccg cagcagcgtg ccgatcggct cgccgcgcgc gacgcgccgc
     7621 agcacctcgg gctcgcgccc ccaggcgagc agcgtcgccg tgcccgagcg cgcggctttc
     7681 gccgccgcgc gcaccttggt cagcatgccg ccacgcccga gcgtaccggc gccgcccccg
     7741 gccctccgtt cgagcgccgg atcgccggcg ctgacctcgg acaggaagcg ggcatcggca
     7801 tgcgcgcgcg gatcgcggtc gtacatgccg tgctggtcgg tcaggatcac gtacagctcg
     7861 gcctcgacca gattcgcgac cagcgcgccg agcgtgtcgt tgtcgccgaa gcggatctcc
     7921 tcgaacgcga ccgtgtcgtt ctcgttgatg accggcacca cgcgcagccg cgtcagcgtg
     7981 cgcagcgtgt tgcgggcgtt caggtagcgc tggcggtcgg agacatcctc gtgcgtcagc
     8041 aggatctgcg cggtgcggat gccgtgcttc tggaacgccg cctcccagcc ctgcaccagc
     8101 cccatctgac cgacggctgc cgccgcctgc agctcggcga gcgccttcgg ccgcgcgcgc
     8161 cagccgagcc ggcgcatgcc ttcggcgacc gcgccggagg aaacgatgat cacgtcccgg
     8221 ccctcggcgt gcagctcggc gagctgcgcg acccaggctt cgatcgccgc ggcatcgaga
     8281 ccgcg
//
LOCUS       DAOQJH010000015         8190 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_4727_length_8190_cov_3.757468, whole genome shotgun sequence.
ACCESSION   DAOQJH010000015 DAOQJH010000000
VERSION     DAOQJH010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 8190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8190
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4727_length_8190_cov_3.757468"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..382)
                     /locus_tag="PLQ18_00745"
     CDS             complement(<1..382)
                     /locus_tag="PLQ18_00745"
                     /inference="COORDINATES: protein motif:HMM:NF014722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase SelO family protein"
                     /protein_id="HNE00203.1"
                     /translation="MAICGLEPVPAGDDDAGMSPLDRLPFDNRTARLPETLFARVAAQ
                     PHGRHHLVHASADALALLDLPPDITADPDFVEVFSGRRVPAGAEPIATVYAGHQFGIW
                     VPQLGDGRALLLGERVNRRGERWEV"
     gene            complement(392..2197)
                     /locus_tag="PLQ18_00750"
     CDS             complement(392..2197)
                     /locus_tag="PLQ18_00750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE00204.1"
                     /translation="MPGFARCLVLLLCLLPWTSVPAAAETTKRPPEPPRPTLVGLVAG
                     GIGGSYARLADDLAAVLDAPDLRILPILGRGSVQNLRDLQALPFVDLALVQSDVLEHL
                     RADPAQEPLVRRLQVIAKLYNEEFHLLVRGHITRLSDLTGTKVNFDVPGSGTEITAAG
                     VFAALKLPVEPTHFDQLTALEKLRSGEISALAFVAGKPAPLFQRVRPEDDLHFLPITL
                     PPELLGRYLPTRFTKADYPNLIVGGDEIQTVAVGALLVTQATRKRAQLRKLELFTGRF
                     FDSFASFRQAGRHPKWREVSIAAQVPDWPRLKAAEDWLATNPDKVRSYAELAARAAAK
                     AEAARQEAARAETARIEAEKAEAARKAEVARIEAEKAEAARKAEIARIEAEKAEAARI
                     EAEKAEAARKAEIARIEAEKAEAARKAEAARVEAEKAEAARRAEAEKAEAARAEAARR
                     AEAEKAEAAKAEAAKQAEAAKAEADKAEAARAEAAKQAEAAKAEADKAEAARKAETAK
                     AEADKAEAARRAEAARAEAATKAEAARKADAAKAEAARKAEAEKAEATKAEADKTEAA
                     RKAEAARSAADRAEAERSGAESLPLTPVNPAPVTP"
     gene            complement(2333..2980)
                     /locus_tag="PLQ18_00755"
     CDS             complement(2333..2980)
                     /locus_tag="PLQ18_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HNE00205.1"
                     /translation="MHIILVANPKGGSGKTTLAVNLAGYYAAHGRRTGLFDLDRQQSA
                     LRWLARRPANLTRISGFDAREDDTVIEDSAGLDVAIIDAPAALRGERFKDIVRIADHA
                     IIPVQPSVFDIDASAAFLERLIEQKSVRKARCLPGLIGNRVQARARSARQLETFIAGR
                     GLPALGALRDSQVYVQTAASGITLFELPGRRQAADRASWQPLLDWLAIPAADPPD"
     gene            3270..6044
                     /locus_tag="PLQ18_00760"
     CDS             3270..6044
                     /locus_tag="PLQ18_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019873634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HNE00206.1"
                     /translation="MDELDDIVREFLAESNENLDRLDNEFVMLESSPDDRDTLASIFR
                     TIHTIKGTCGFLGFSHLEKVTHAGENLLSKLRDGHIRLNQERTTALLQMVDAVRAMLA
                     AIESTGSDGDQNYPELTERLLTLQRKEETVVAPVAKTPAPPPPPPVPTEVAEGMDEIV
                     VEFLTESQENLDRLDNEFVALEAAPDDRDTVASIFRTIHTIKGTCGFLGFSKLEKLTH
                     AGENLLSKLRDGRIRLNQERTTALLKMVDAVRVMLGSIGASGHDGNETYPELIELLTA
                     LQSDGAAPAAAAPAAKPVVPALGEILVQQGVVAPERVDAALSKQDAGDPRHVGEILVQ
                     EEAVKPAAVVEALKAQKDVAKEVKAPAVAETSIRVDVGLLDHLMNLVGELVLTRNQVL
                     QYSKSADDSRFAATSQRLNLITTELQEGVMKTRMQPINNIWNKVPRVVRDLALGVSKQ
                     IRVEMVGKETELDKSLIEAMKDPLTHLVRNCVDHGIEAPDVRVQKGKPAEGVLTLRAY
                     HEGGQVIVEIADDGAGIDPQKLKSKALQKGLITAEQAEAMSARELINLIFLPGFSTAE
                     KVTNISGRGVGMDVVKTNIEKISGNIELRSELGHGTLFKINLPLTLAIVPALIVTSGG
                     QRFAIPQGSLQELIRLDAAQGIEQIHGAHVYRLRGKLLPLVYLNRELGLEGATRNANI
                     VVLQSDKRRFGLVVDEINDTQEIVVKPLSKLLKGLTVFAGATVMGDGRVVLILDILGL
                     AQRARIVGETRDHGAAESIANGDVSGEAQNMLLVRSPGDGRLAIALSKVARLEEFPRN
                     SLEMAGSQEVVQYRKQILPLVRLEDVLPERRRFDRYEDMATLDESVVQVVVYTVNGRS
                     LGLVVDQIIDIIEENLDLTGDPSREGVLGTTVLQGRVTEVFDVDGFLERMFAALFPAR
                     PTARLSSTVY"
     gene            6044..6484
                     /locus_tag="PLQ18_00765"
     CDS             6044..6484
                     /locus_tag="PLQ18_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010582840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HNE00207.1"
                     /translation="MSDDAQYCTFRLGSLFFGVEVTKVQEVIRYQEMTPIPLAQRTVS
                     GLINLRGQIVTAIDLRRVLEFEDRPADERPMNVVIRTDEGAVSLLVDRIGDVMHVDSD
                     SFERVPDTVTGVARDLILGAYKLPDTLLLVLDTDRIVGSDDGSH"
     gene            6754..7926
                     /locus_tag="PLQ18_00770"
     CDS             6754..7926
                     /locus_tag="PLQ18_00770"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012950954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HNE00208.1"
                     /translation="MDRIRILIVDDAVVVRRMLSTLLAEDPELEVVGTAVNGADALRK
                     IPDLRPHLLILDVDMPEMNGLETLTVLGREHPRLPVIMFSNLTRRGAQTTLEALALGA
                     KDYVTKPESVGSAGEALEQVRAALVPKIKALCRPWEPAPAVLARRSHEPPAARQAPPS
                     APAPAPAAPVSPAAHASAPPLPSPVLAPMTRRGGIELVVIGCSTGGPNALATLVAALP
                     RGLAVPVVVVQHMPPVFTRMLAERLDALSPLSVREGSDGARIQPGEIWIAPGDFHMTV
                     RRSADGLHLGLNQGAPENSCRPAVDVLLRSAVAACGARVLGVILTGMGHDGLASSTRL
                     REAGGQLLAQDERSSVVWGMPGNVVRAGLADRVLAIEDIGPEIAARVRGEGGRHGC"
     gene            7920..>8190
                     /locus_tag="PLQ18_00775"
     CDS             7920..>8190
                     /locus_tag="PLQ18_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012950953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE00209.1"
                     /translation="MLTATDFDYVRRLVLERSAILLEPGKEYLVELRLKPIVRMRGLR
                     DLGELVQLLRTDVSGSLQREVVEAMTTNETSFFRDVHPFETLRQWV"
BASE COUNT         1172 a         2812 c         2963 g         1243 t
ORIGIN      
        1 gcacctccca gcgctcgccg cggcggttga cgcgctcgcc gagcagcagc gcgcggccgt
       61 cgccgagctg cggcacccag atgccgaact ggtgaccggc gtagacggtc gcgatcggtt
      121 cggcgccggc cggcacgcgc cgcccggaga acacctcgac gaagtccggg tccgccgtga
      181 tgtcgggcgg caggtcgagc agcgccaggg cgtcggcgct ggcgtggacc aggtgatggc
      241 ggccgtgcgg ctgcgcggcg acgcgtgcga acagcgtctc cggcagccgg gcggtacggt
      301 tgtcaaacgg caggcgatcg agcgggctca tgccggcatc gtcgtcgccg gccggcacgg
      361 gttcaagccc gcagatcgcc acgccgccgg ttcagggcgt caccggggcc ggattcaccg
      421 gcgtcagcgg cagggactcg gcgccgctgc gttcggcttc cgcccggtcg gcggcgctgc
      481 gggccgcctc ggccttgcgg gcggcctcgg tcttgtcggc ttcggccttc gtcgcttcgg
      541 ccttttcggc ttcagccttg cgcgcggctt cggccttcgc cgcatcggcc ttgcgcgcgg
      601 cctcggcctt ggtggccgct tcggcgcggg cggcctcggc cctgcgcgcg gcttcggcct
      661 tgtcggcctc ggccttcgcg gtttcggcct tgcgggcggc ttcggccttg tcggcctcgg
      721 ccttcgcggc ctcggcctgc ttcgcggctt cggcgcgcgc cgcttcagcc ttgtcggcct
      781 cggccttcgc agcctcggcc tgcttcgcgg cctcggcctt cgcggcttcg gccttttcgg
      841 cttcggcgcg gcgtgcggcc tcggcgcgcg ccgcctcggc cttctcggct tcggccctgc
      901 gtgcggcctc ggccttctcg gcctcgacgc gggcagcctc ggccttgcgt gcggcctcgg
      961 ccttttcggc ctcgatgcgg gcgatctcgg ccttgcgggc ggcttcggcc ttctcggcct
     1021 cgatgcgtgc ggcctcggcc ttctcggcct cgatgcgggc gatctcggcc ttgcgcgcgg
     1081 cctcggcctt ctcggcctcg atgcgggcga cctcggcctt gcgggcggct tcggccttct
     1141 cggcctcgat gcgggcggtt tcggcgcggg cggcctcctg acgggcggcc tcggccttgg
     1201 cggcggcgcg ggcggcgagt tcggcgtagc ttcggacctt gtcggggttc gtcgccagcc
     1261 agtcctcggc ggccttcagc cgcggccagt ccgggacctg ggcggcgatg ctgacctcgc
     1321 gccacttcgg atgccggccg gcctgacgga agctggcgaa gctgtcgaag aagcggccgg
     1381 tgaacagttc gagcttgcgc agctgcgcgc gcttgcgcgt cgcctgcgtg accagcagcg
     1441 cgccgacggc gacggtctgg atctcgtcgc cgccgacgat caggttcgga tagtcggcct
     1501 tcgtgaagcg cgtcggcaga tagcgcccga gcagttccgg cggcagcgtg atcggcagga
     1561 agtgcaggtc gtcctcgggg cgcacgcgct ggaacagcgg tgcgggcttg ccggcgacga
     1621 aggcgagcgc gctgatctcg ccgctgcgca gtttctcgag cgcggtgagc tgatcgaaat
     1681 gcgtcggctc gaccggcagc ttcagcgcgg caaagacgcc ggcggcggtg atctcggtgc
     1741 cgctgcccgg cacgtcgaag ttgaccttgg tgccggtcag gtcgctcagg cgggtgatgt
     1801 ggccgcgcac cagcaggtgg aattcctcgt tgtacagctt ggcgatcacc tgcagccggc
     1861 gtacgagcgg ctcctgcgcc gggtctgcgc gcaggtgttc gagcacgtcg gactgcacca
     1921 gcgccagatc gacgaacggc agcgcctgca ggtcgcgcag gttctgcacc gagccgcgcc
     1981 cgaggatcgg caggatgcgc aggtccgggg cgtcgagcac ggcggcgagg tcatcggcca
     2041 gccgcgcgta actgccgccg atgccgccgg ccaccagtcc gaccagcgtc gggcgcgggg
     2101 gctccggcgg gcgtttcgtg gtttccgcgg ccgccgggac cgaagtccag ggcagcaggc
     2161 acagcagcag gacgaggcaa cgggcgaaac ctggcatcgg agcgggctcc acggctcggg
     2221 gacgggcgcg tcggcacggg acgcgggggc gggatcggac ggcggggcgg gcaagggggc
     2281 cgggtgcgga gccgtccgga tcagatccgg atccggtccg catcacggtg gctcagtctg
     2341 gcggatcggc ggccggtatc gccagccagt cgagcagcgg ctgccagctc gcccgatcgg
     2401 cggcctggcg ccggccgggc agctcgaaca gcgtgatgcc gcttgcggcc gtctgcacgt
     2461 agacctgcga atcgcgcagc gcgccgagcg ccggcaggcc gcgcccggcg atgaaggttt
     2521 cgagctggcg cgccgagcgc gcccgcgcct gcacgcggtt gccgatcagc cccggcaggc
     2581 agcgcgcctt gcgcacggat ttctgttcga tgagccgctc aagaaatgcc gcgctcgcgt
     2641 cgatatcgaa gaccgagggc tgcacgggga tgatggcgtg atcggcaatg cggacgatgt
     2701 ccttgaaacg ctcgccgcgc agggcggccg gtgcatcgat gatggcgacg tcgagtccgg
     2761 cgctgtcctc gatcaccgtg tcgtcttcac gggcgtcgaa gccgctgatg cgcgtcaggt
     2821 tcgccggccg gcgggccagc cagcgcagcg ccgactgctg acggtccaga tcgaacaggc
     2881 cggtgcgccg gccgtgggcc gcgtagtagc cggcgaggtt gacggcgagc gtggtcttgc
     2941 cggagccgcc cttggggttg gcaaccagga tgatgtgcat ggcgctgcga cctcggctgc
     3001 tggccggtgc tgcccgttcg gcgcgcgact tgtagcgggc gcgggccgct gctggcaagc
     3061 cttgcgcagg gcggggcggg atgccgctcc gggaaccgga ctgcagccgg cccttgtcgc
     3121 attgtggaat tcacctagct tgacaggccg gtgctacggc ccggcctgac cggtgtgcta
     3181 tttcggtgcc cccggagcgt gcgtgcgccg ctcccgcgca acgcagcgga ttcacctgcc
     3241 cgggccgacc cggcgaggaa cacagagcga tggatgaact tgacgacatc gtccgcgaat
     3301 tcctggcgga aagtaacgag aacctggacc ggctcgacaa cgagttcgtg atgctggagt
     3361 cgagccccga cgaccgggac acgctcgcga gcatcttccg cacgatccac acgatcaagg
     3421 gcacctgcgg cttcctcggc ttttcgcatc tcgaaaaggt cacgcacgcc ggcgagaacc
     3481 tgctctcgaa gctgcgcgac ggccacatcc gcctgaatca ggagcgcacg acggcgctgc
     3541 tgcagatggt cgatgccgtg cgcgcgatgc tcgcggcgat cgagagcacc ggcagcgacg
     3601 gcgaccagaa ctacccggaa ctcaccgagc gcctgctgac gctgcagcgc aaggaggaaa
     3661 cggtcgtggc accggtggcg aagaccccgg cgccgccgcc gccgccgccg gtgccgaccg
     3721 aggtggccga gggcatggac gagatcgtcg tcgagttcct gaccgagagt caggagaacc
     3781 tcgaccgcct cgacaacgag ttcgtcgcgc tcgaggccgc gcccgatgac cgcgacacgg
     3841 tcgcgagcat cttccgcacc atccacacga tcaagggcac ctgcggtttc ctcggcttct
     3901 cgaagctcga aaagctcacg cacgccggcg agaacctgct ctcgaagctg cgcgacggtc
     3961 gcatccgcct gaatcaggag cgcacgacgg cgctgctgaa gatggtcgat gccgtgcgcg
     4021 tgatgctcgg ctcgatcggt gcgagcggcc acgacggcaa cgagacctat ccggagctga
     4081 tcgagctgct gacggcgctg cagagcgacg gcgcggcccc ggcggcggcc gcgccggctg
     4141 cgaagccggt ggtgccggcc ctcggcgaga tcctcgtgca gcagggcgtg gtcgcgcccg
     4201 agcgcgtcga tgccgcgctg tcgaagcagg atgccggcga tccgcgccac gtcggcgaga
     4261 ttctcgtgca ggaggaggcc gtgaagccgg cggccgtggt cgaggcgctg aaggcgcaga
     4321 aggacgtcgc gaaggaggtc aaggcgccgg cggtggccga gacctcgatc cgcgtcgatg
     4381 tcggcctgct cgatcacctg atgaacctcg tcggcgagct ggtgctgacc cgcaaccagg
     4441 tgctgcagta ctcgaagtcc gccgacgatt cgcgcttcgc ggcgacctcg cagcggctga
     4501 acctgatcac gaccgagctg caggaaggcg tcatgaagac gcgcatgcag ccgatcaaca
     4561 acatctggaa caaggtgccg cgcgtcgtgc gcgacctcgc gctcggcgtc agcaagcaga
     4621 tccgcgtcga gatggtcggc aaggaaaccg aactcgacaa gtcgctgatc gaggcgatga
     4681 aggacccgct gacccacctc gtgcgcaact gcgtcgatca cggcatcgag gcgccggacg
     4741 tgcgcgtgca gaagggcaag cccgccgagg gcgtgctgac gctgcgcgcc taccacgagg
     4801 gcgggcaggt catcgtcgag atcgccgatg acggtgccgg catcgatccg cagaagctca
     4861 agtccaaggc gctgcagaag ggcctgatca cggccgagca ggccgaggcg atgtcggcgc
     4921 gcgagctgat caacctgatc ttcctgcccg gcttctcgac cgccgagaag gtcacgaaca
     4981 tctccggccg cggcgtcggc atggacgtgg tcaagaccaa catcgagaag atcagcggca
     5041 acatcgagct gcgcagcgag cttggtcacg gcacgctgtt caagatcaac ctgccgctga
     5101 cgctggcgat cgtgccggca ctgatcgtga cctccggcgg ccagcgcttc gcgatcccgc
     5161 agggcagcct gcaggagctg atccgcctcg acgccgcgca gggcatcgag cagatccacg
     5221 gtgcccacgt ctaccggctg cgcggcaagc tgctgccgct cgtgtacctg aaccgcgaac
     5281 tcgggctcga aggcgcgacg cgcaacgcca acatcgtcgt gctgcagtcc gacaagcgcc
     5341 gcttcgggct ggtggtcgac gagatcaacg acacgcagga aatcgtcgtc aagccgctct
     5401 cgaagctgct caagggcctg actgtgttcg ccggagccac ggtcatgggc gatggccgcg
     5461 tcgtgctgat cctcgacatc ctcggcctcg cgcagcgtgc gcgcatcgtc ggcgagacgc
     5521 gcgaccacgg tgcggccgaa tccatcgcca acggcgacgt gagcggcgag gcgcagaaca
     5581 tgctgctggt gcgctcgccc ggcgacggac ggctcgcgat cgcgctgtcg aaggtcgcgc
     5641 gcctggagga attcccgcgc aattcgctgg agatggccgg ctcgcaggag gtcgtgcagt
     5701 accgcaagca gatcctgccg ctggtgcgcc tcgaggacgt gctgcccgag cgccggcgct
     5761 tcgaccgcta cgaggacatg gccacgctcg acgagagcgt cgtgcaggtc gtcgtctaca
     5821 cggtcaacgg ccgcagcctc ggcctggtcg ttgaccagat catcgacatc atcgaggaga
     5881 acctcgacct gaccggcgat ccttcgcgcg agggcgtgct cggcacgacg gtgctgcagg
     5941 gccgtgtcac cgaggtcttc gatgtcgatg gtttccttga acgcatgttc gccgcgctgt
     6001 tcccggcccg gccgactgcc cgcctgagca gcacggtgta ctgatgagcg acgacgcgca
     6061 gtactgcacc ttccgcctcg gcagcctgtt cttcggggtg gaagtcacga aggtccagga
     6121 agtcatccgc taccaggaga tgacgccgat cccgctcgcc cagcgcacgg tctcggggct
     6181 gatcaacctg cgcggccaga tcgtcacggc gatcgacctg cgccgggtgc tcgaattcga
     6241 ggaccgtcca gccgacgaac ggccgatgaa cgtcgtgatc cgcaccgacg aaggcgcggt
     6301 cagcctgctg gtcgatcgca tcggcgatgt gatgcacgtc gattccgaca gcttcgagcg
     6361 cgtgcccgac accgtcaccg gcgtggcccg cgatctgatc ctcggtgcct acaagctgcc
     6421 ggacacgctg ctgctggtgc tcgacaccga ccgcatcgtc ggcagcgacg acggcagcca
     6481 ctgaagttcc ggcccccggt ttccggccgc aggcaggcac aagaatcatg gacagaccgg
     6541 ctcgggtgcg ctttgctgcg cggcccgggt cgctccgggt ctgcgcggct ggcgacgggg
     6601 ccggtggcga ccgaaaccga accgcaccgc aggcgtcggg ccccggagag ggcaacgatg
     6661 gatgaatacg tgccgaagcc atatccgggt gccatgccgg gcccgaccca cggcctgccc
     6721 ggcctgacgt gactgccgct tccggagccc gccttggacc gcatccgcat cctcatcgtc
     6781 gatgacgcag tcgtcgtgcg gcgcatgctg tcgacgctgc tcgccgaaga ccccgaactg
     6841 gaggtcgtcg gcacggccgt caacggcgcc gatgcgctgc gcaagattcc cgatctgcgg
     6901 ccgcatctgc tgatcctcga tgtcgacatg ccggaaatga acggcctgga gacgctgacc
     6961 gtgctcgggc gcgagcatcc gcggctgccg gtgatcatgt tcagcaacct gacgcggcgc
     7021 ggcgcgcaga ccacgctcga agcgctggcc ctgggcgcca aggactacgt gaccaagccc
     7081 gagagcgtcg gcagtgccgg cgaggcgctg gagcaggtgc gcgccgcgct ggtgccgaag
     7141 atcaaggcgc tgtgccggcc ctgggagccg gcaccggcgg tgctggcccg gcgcagccac
     7201 gaaccgcctg cagcccggca ggcgccgccc tccgcccctg cgcctgcacc ggccgctccg
     7261 gtttcccccg ccgcgcatgc ttccgcgccc ccgctgccgt ccccggtgct ggcgccgatg
     7321 acccggcgcg gcggcatcga actggtcgtg atcggctgtt cgaccggcgg tccgaacgcg
     7381 ctcgccaccc tggtcgcggc gctgccgcgc gggctggcgg tgccggtggt ggtcgtgcag
     7441 cacatgccgc cggtgttcac gcgcatgctg gccgagcggc tcgatgcgct gtcgccgctg
     7501 agcgtgcgcg agggcagcga tggcgcgcgc atccagcccg gggagatctg gatcgcgccc
     7561 ggtgatttcc acatgacggt gcgccgctcg gccgatggcc tgcatctggg gctcaatcag
     7621 ggggcgcccg agaactcctg ccggccggcg gtcgacgtgc tgctgcgctc ggcggtggcc
     7681 gcctgcggcg cccgcgtgct cggcgtgatc ctgaccggca tgggccacga cggcctggcc
     7741 tcgtcgacgc gcctgcgcga ggccggcggc cagctgctcg cgcaggacga gcgcagcagc
     7801 gtcgtctggg gcatgccggg caacgtcgtg cgggccggac tcgccgatcg cgtgctggcg
     7861 atcgaggaca tcggcccgga aatcgccgcc cgtgtgcgcg gcgaaggcgg caggcacgga
     7921 tgctgaccgc gaccgatttc gactacgtgc gccggctggt gctggagcgc tcggcgatcc
     7981 tgctcgaacc gggcaaggaa tacctggtcg agctgcgcct gaagccgatc gtgcgcatgc
     8041 gcggcctgcg tgatctcggc gagctggtgc agctgctgcg cacggacgtg agcggcagcc
     8101 tgcagcgcga ggtcgtcgag gcgatgacga ccaacgagac ctcgttcttc cgcgacgtgc
     8161 atccgttcga gaccctgcgc cagtgggtgc
//
LOCUS       DAOQJH010000016         8160 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_4748_length_8160_cov_3.999136, whole genome shotgun sequence.
ACCESSION   DAOQJH010000016 DAOQJH010000000
VERSION     DAOQJH010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 8160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8160
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4748_length_8160_cov_3.999136"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1285
                     /locus_tag="PLQ18_00780"
     CDS             <1..1285
                     /locus_tag="PLQ18_00780"
                     /inference="COORDINATES: protein motif:HMM:NF040606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="di-heme-cytochrome C peroxidase"
                     /protein_id="HNE00210.1"
                     /translation="IKGVEVRVDGGPGLIDFDGFMNGMVAAMDRAAASPDAFSRFAAE
                     VLGSNATPEKLADLRTAFGEHTQFLKTRIAANAIPRPAGHGRVDAFGQIFNQAVVHAL
                     GVPGNAHPADAPVSYPFIWDAPQHDRLQWNGSALNLGAGPLARNSGEVVGVFGTVNVN
                     QPGFGMRGYASSLDTQQLDGLERLLTTLWSPEWPSKYAGPLDATLVARGKTLFAANCA
                     GCHQNIDAKGSVTFDRADPNRRITAQMIDLGKVGTDPHMAKNVLACTADTGPLNGRPV
                     AVLPFGPKLKPKDLCVNVTGNVVVGAILGNNPPVSLFQVLEFLKGSQALKAKLMNPQY
                     KARPLNGIWATAPYLHNGSVPTLADLLQPPAQRPKTFHVGDREFDAGKVGYVTDRGPF
                     VYDTAEPGNSNAGHLYGTKLATADKRALLEYLKSL"
     gene            1424..2884
                     /gene="astD"
                     /locus_tag="PLQ18_00785"
                     /pseudo
     CDS             1424..2884
                     /gene="astD"
                     /locus_tag="PLQ18_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011135051.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
     gene            3225..3620
                     /locus_tag="PLQ18_00790"
     CDS             3225..3620
                     /locus_tag="PLQ18_00790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE00211.1"
                     /translation="MPIVIASSFADPADIAAYKKAIAAVKTEAEALKVGDNGIGKWGD
                     DTTREDVPICALPPEDWEGKWGKGDTARGKKVAVTYKGKTVIGELRDTMPRKANIKNG
                     AGIDLNPGFAKAFGVRQPFMLKDVTWEWA"
     gene            complement(3752..4804)
                     /gene="arsB"
                     /locus_tag="PLQ18_00795"
     CDS             complement(3752..4804)
                     /gene="arsB"
                     /locus_tag="PLQ18_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006366995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HNE00212.1"
                     /translation="MASVSRRLSFLDRWLTLWIFAAMAIGVAWGHFFPGIAGFWQGLS
                     SGTTSWPIAIGLIVMMYPPLARVRYEALPQVFRNTRVLLLSLLQNWVIGPLLMFALAV
                     LLLPDQPHYMAGLILIGLARCIAMVIVWNELARGDTEYCAGLVAFNALFQVLFFPVYA
                     WLFLSVLPGWLGLETFKVEVSIGEVAWTVFVYLGIPFLAGFVTRLVLRRMKGDDWYRT
                     VFVPRISPLTLIALLFTILVMFSLKGELIVRLPLDVLRIAVPLAIYFVVMFLLSFALG
                     RRYGAPYGQTATLSFTAASNNFELAIAVAVAVFGLNSGEAFAAVIGPLVEVPALIGLV
                     HVAGWLQRRWFVAAVA"
     gene            complement(4818..5342)
                     /locus_tag="PLQ18_00800"
     CDS             complement(4818..5342)
                     /locus_tag="PLQ18_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020206787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsI/CadI family heavy metal resistance
                     metalloenzyme"
                     /protein_id="HNE00213.1"
                     /translation="MKRFHVHLAVEDLAASVRFYTALFGCGPGVLKDDYAKWMLEDPR
                     VNFAISARGRAVGLDHLGFQVGDAEELAAAYAQLAQAGAPVIEQAGTTCCYAKSDKQW
                     VHDPQGIAWETFLTLGEATHYGADLPATATACCAPPAAPAKPVAIAVVAGAPEGGCCT
                     PKAGSGSGSASGCC"
     gene            complement(5437..5946)
                     /locus_tag="PLQ18_00805"
     CDS             complement(5437..5946)
                     /locus_tag="PLQ18_00805"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012112894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HNE00214.1"
                     /translation="MSPTPAAHVHHVLFLCTGNSARSILAEALLNHWGGGRFRAYSAG
                     SFPVGRVNPRALALLEHLGMPTEGLRSKSWDEFAGPEAPALDFVFTVCDAAAGEVCPI
                     WPGHPVTAHWGVADPAAVEGSETEQMAAFREAFRVLENRIKAFTALRPEALDRLALKQ
                     QVDAIGRQG"
     gene            complement(6110..6451)
                     /locus_tag="PLQ18_00810"
     CDS             complement(6110..6451)
                     /locus_tag="PLQ18_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005669588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNE00215.1"
                     /translation="MDQTEAVAALGALAQDSRLAIFRLLVQAGPPGLPAGQIGERLGL
                     APATLSFHLKTLRAAGLVEQRRDGRSLIYAADFARMQALLGYLTEHCCQDDPGVCGLA
                     APTNEDCGCGG"
     gene            complement(6835..>8160)
                     /locus_tag="PLQ18_00815"
     CDS             complement(6835..>8160)
                     /locus_tag="PLQ18_00815"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNE00216.1"
                     /translation="RSIRAVLADPAAAVPLPGARRPPAPVPLWLHPAPPRPQSKLTRP
                     LADPPAEAASGHTGPDDAARKRQAERVDDPDGKRGLLAFRLESMFSWTEYVKVDRCDD
                     DSEDDQACSNADDLRVLSVARGKAPATKLRFDLDLPSAGQDDIPLGDGIPVPEWDWKR
                     GVLLPDHCRVQLMLPRGAVPAELPAHLRVTARRLRAQFEQLRPVRSWLRGLPDGADID
                     LDAWLAHRAERMNGTASGDVRLYRDFRGGVRDLACLLLADLSLSTDAWVGEHGRVIDV
                     IRDALHLFAEALAATGDRYALYGFSSRRRDHVRLLALKAFDERHDAGIRGRIAAIRPG
                     YYTRMGAAIRHATTLLGREPASRRILILLTDGKPNDLDRYEGRWGIEDTRAAIREARE
                     AGLQPFCVTIDEQAHDYLPYLFGTQGWVLIRKPAELPRELPLLYARLTG"
BASE COUNT         1350 a         2902 c         2794 g         1114 t
ORIGIN      
        1 catcaaaggc gtcgaggtgc gcgtcgacgg cggccccggg ctgatcgatt tcgacggctt
       61 catgaacggc atggtcgcgg cgatggaccg cgccgctgcc agccccgacg ccttctcgcg
      121 cttcgccgcc gaggtgctcg gcagcaacgc cacgcccgaa aaactcgccg acctgcgcac
      181 ggccttcggc gagcacacgc agttcctgaa gacgcgcatc gccgccaacg ccatcccgcg
      241 cccggccggc cacggccgcg tcgatgcctt cggccagatc ttcaaccagg cggtcgtgca
      301 cgcgctcggc gtgcccggca acgcccaccc ggccgatgca ccggtctcgt acccgttcat
      361 ctgggatgcg ccgcagcatg atcgcctgca gtggaacggc agcgcactga acctcggggc
      421 cgggccgctg gcgcgcaact ccggcgaggt ggtcggcgtg ttcggcaccg tgaacgtgaa
      481 ccagcccggc ttcggcatgc gcggctatgc gtcctcgctc gacacgcagc agctcgacgg
      541 cctcgaacgc ctgctgacga cgctgtggtc gcccgaatgg ccgtcgaagt acgccggccc
      601 gctcgatgcg acgctggtcg cgcgcggcaa gacgctgttc gccgccaact gcgcgggctg
      661 ccaccagaac atcgacgcga agggcagcgt caccttcgat cgtgccgatc cgaaccgccg
      721 catcaccgcg cagatgatcg acctcggcaa ggtcggcacc gatccgcaca tggcgaagaa
      781 cgtgctcgcc tgcaccgccg acaccggccc gctgaacggc cggccggtcg cggtgctgcc
      841 gttcgggccg aagctcaagc cgaaggatct gtgcgtgaac gtcaccggca acgtcgtcgt
      901 cggcgcgatt ctcggcaaca acccgccggt cagcctgttc caggtgctgg agttcctgaa
      961 aggcagccag gccctgaagg ccaagctgat gaacccgcag tacaaggccc ggccgctgaa
     1021 cggcatctgg gcgacggcgc cctacctgca caacggctcg gtgccgacgc tcgccgacct
     1081 gctgcagccg ccggcgcagc gtccgaagac cttccacgtc ggcgatcgcg aattcgacgc
     1141 cggaaaggtc ggctacgtca ccgaccgcgg tccgttcgtc tacgacaccg ccgagccggg
     1201 caacagcaat gccggccacc tgtacggcac gaagctcgcc acggccgaca agcgcgcgct
     1261 gctggagtac ctgaagtcgc tgtaagcgcc gtttctgcac ccgccagccc cgccgacggc
     1321 ggagcctccc ggaagcggag gctccgccgt cggtttttgc gccgcgtgcc gggctcgcgg
     1381 cgactacgct cgcgcttcaa caccggcggg agaacgtgca gcgatgactc atctgatcgg
     1441 cagcgactgg cagcccggcg gcggcgaatc cttcgcgtcg gaaaaccccg cgaccggcgc
     1501 gatgctctgg cgcggccatg ccgccgatgc cgccgatgtc gatgccgcgg tgcgcgccgc
     1561 acgcgcggcc ttcccggcct gggcggccct ggacttcgag gcgcgcgtcg aacggctgcg
     1621 cgccttcgag gccacgctgc aggccaacgc cccggaactc gccggcacca tctcggccga
     1681 gaccggcaag ccgcgctggg aaacggcgac cgaggtcgcg gcgatgatcg ccaagatcgg
     1741 catctcgatc gccgcgcacg ccgagcgcac cggcacgcgc gaaggcacgg ccgccggcgt
     1801 gcgcgcggtg ctgcgccacc ggccgcacgg cgtggtcgcg gtgttcggcc cgtacaactt
     1861 ccccggccac ctgccgaacg gccacatcgt gccggcgctg ctcgccggca ataccgccgt
     1921 gttcaagccg agcgagcaga caccgctggt cgccgagcgc accgtgcagc tctggcaggc
     1981 ttcgggcctg ccggccggcg tgctgaacct cgtgcacggc gcgcgtgcga ccgggcaggc
     2041 gctggccacg catccggaac tcgacggcct gttcttcacc ggcagccacg ccaccggcga
     2101 atggctgcac cgcagctttg ccgggcgcac cggccgcatc ctcgcgctcg aactcggcgg
     2161 caacaatccg ctgatcgccg atgccgtcac gctctccgac ctcgacgccg cctgctggca
     2221 catcgtgcag tcggcctgga tcaccgccgg ccagcgctgc acctgcgcac gccggctgat
     2281 cgtgccgctc ggggcacgcg gcgatgcgct gatcgaacgg ctctgccagt ggctcgacgg
     2341 catccgcatc ggcgcgcccg atgacgaacc cgagccgttc atggggccgc tgatctcgaa
     2401 cgccgccgcc gatgcgctgc tgaacttcca gaccgatctc gaactggccg gcgcccgggt
     2461 gctgcgccgc gcccagcgcc tcgaccccgc ccgtccgctg ctgacgccgg cgctgctcga
     2521 cgtgagcgcc tgcagcctgc gccccgacgc cgaagccttc ggcccgctgc tgcagctcgt
     2581 gcgcgtcgcc gacttcgaga ccgcgctctg ggaggccaac cacacgcgct tcggcctcgc
     2641 cgccgggctg ctgtccgacg atgcgacgcg ctggcagcag ttccgcacca cgatccgcgc
     2701 cggcatcgtc aactggaacc gcccgaccac cggtgcggcc agcagcgccc cgttcggcgg
     2761 catcggcgac agcggcaacc tgcgcccgag cgcgtattac gcggccgact actgcgcgta
     2821 tccggtcgcg tcgctggagg cggatacgct cacgcggccg gcgcaggcag ggacggggct
     2881 ggtgccgccg gcacgagaca accgcagcgc ccgcccgggc caccgcgacg ggatggacgc
     2941 tgcaaacccg gcgcgtcagg ggggcggtaa ttctcgctgc gggatgcagg ccggggtcgg
     3001 aatgcgcctg cgcggtgcag gcagtcgatg gcgcgtactg gaatcccgac gctatagttc
     3061 atcggcgcac ccgcctgcgt gtttccgggt acttttgcag acgctggttt gctggcatga
     3121 cgcggaaggc ggattacggt gtgcggaaag gatggtgagc cttgatacgc cacgtaatgt
     3181 gcacctgagc cgccctgcgt gcccaataca tcgaaggaac tccaatgcct atcgtaatcg
     3241 cttcatcctt cgctgaccct gccgacatcg cggcatacaa gaaggctatt gctgccgtta
     3301 aaaccgaggc agaggcgctt aaagttggcg ataacggaat cggcaaatgg ggagatgaca
     3361 caacgcgaga agacgtcccg atatgtgcgc tgcctccaga ggattgggaa ggcaagtggg
     3421 gcaagggcga taccgccaga ggcaaaaagg ttgccgtaac atacaaaggc aagacggtta
     3481 taggagaact tagggatacc atgccccgca aagcaaacat caagaacggg gctggtattg
     3541 atctgaaccc aggcttcgcg aaggcattcg gagtcagaca accatttatg cttaaagacg
     3601 tcacctggga gtgggcctga cccatctcat tataccaacc acagggccgt aggctgggtt
     3661 gaggaacgaa acccagcatc ggaagctacc ccgtgtgatg actgccgggt ttccttcgtc
     3721 aacccagcct acgggctctg atacgcagcc ctcacgccac cgccgccaca aaccaccgcc
     3781 gctgcagcca gccggcgacg tgcaccaagc cgatcagcgc cggcacctcg accagcgggc
     3841 cgatgacggc tgcgaaggct tcgccggagt tcaggccgaa caccgcgacg gcgaccgcga
     3901 tcgccagttc gaagttgttg ctcgcggccg tgaacgacag cgttgcggtc tggccgtagg
     3961 gcgcgccgta gcgtcgtccg agcgcgaagc tcagcaggaa catcacgacg aaatagatcg
     4021 ccagcggcac ggcgatgcgc agtacgtcga gcggcaggcg cacgatcagt tcgcccttca
     4081 gcgaaaacat cacgaggatc gtgaacagca gcgcgatcag cgtcagcggc gagatgcgcg
     4141 gcacgaacac cgtccggtac cagtcatcgc ccttcatccg gcgcaacacc agccgcgtca
     4201 cgaaaccggc gagaaacgga atgccgagat acacgaacac cgtccacgcc acctcgccga
     4261 tcgagacttc gaccttgaag gtttcaagtc cgagccagcc cggcagcacg ctcaggaaca
     4321 gccacgcata caccgggaag aacagcacct ggaacagcgc gttgaaggcc accagcccgg
     4381 cgcagtactc ggtgtcgcca cgcgcgagct cattccagac gatgaccatc gcgatgcagc
     4441 gcgccagccc gatcaggatc agcccggcca tgtaatgcgg ctgatccggc agcagcagca
     4501 cggcgagcgc gaacatcagc agcggcccga tcacccagtt ctgcagcagc gacagcagca
     4561 gcacgcgcgt gttgcggaac acctgtggca gcgcctcgta gcgcacgcgc gcgagcggcg
     4621 gatacatcat gacaatcagc ccgatcgcga tcggccagct ggtcgtgccg ctcgacagac
     4681 cctgccagaa gccggcgatg ccggggaaga aatgccccca ggccacgccg atcgccatcg
     4741 ccgcgaagat ccacagcgtc agccagcgat cgaggaacga caggcggcgg gagacggaag
     4801 ccatgcgcac gcccccgtca gcagcagccg gaagcggagc cggagccgct gccggccttc
     4861 ggcgtgcagc agccgccttc cggcgccccg gccacgaccg cgatcgcgac cggcttcgcc
     4921 ggggcggccg gcggtgcgca gcaggcagtc gcggtcgccg gcaggtcggc accgtaatgc
     4981 gtcgcctcgc cgagcgtcag gaaggtctcc caggcgatgc cctgcggatc gtgcacccac
     5041 tgcttgtcgg acttcgcata gcagcaggtc gtgccggcct gctcgatgac cggcgccccg
     5101 gcctgcgcga gctgcgcata cgccgcggca agctcttcgg catcgccgac ctggaagccg
     5161 agatgatcga gcccgaccgc ccggccgcgg gcgctgatcg cgaagttcac gcgcgggtcc
     5221 tccagcatcc acttcgcgta atcgtccttg agcacgcccg gcccgcagcc gaacagcgcg
     5281 gtgtagaagc gcaccgaggc ggcgagatcc tcgacggcga gatggacatg gaagcgtttc
     5341 atggcggtgg ctccgggggg ggggaaggaa tcggtggacg gacttcgcag cccgatgaac
     5401 gcaggggctc tgcgcgccct ggccgcggga tcggtatcag ccctggcgcc cgatcgcatc
     5461 gacctgctgc ttcagtgcca gccggtcgag ggcctcgggg cgcagggccg tgaaggcctt
     5521 gatgcggttt tcgagcaccc ggaacgcctc gcggaaggcc gccatctgtt cggtctcgct
     5581 gccctcgacc gccgccgggt cggccacgcc ccagtgcgcg gtgaccggat gccccggcca
     5641 gatcgggcag acttcgccgg ccgccgcatc gcagaccgtg aacacgaaat cgagcgccgg
     5701 cgcctccggc ccggcgaact cgtcccagga cttcgagcgc agcccttcgg tcggcatccc
     5761 cagatgttcg agcagcgcca gcgcgcgcgg attgacgcgc ccgaccggga agctgccggc
     5821 gctgtacgcc cggaagcgtc ccccgcccca gtgattgagc agcgcctcgg cgaggatgct
     5881 gcgggccgag ttgccggtgc agaggaacag cacgtgatgg acgtgagcgg cgggcgtggg
     5941 gctcatgggc aggctccggg ggtggatgca gcgattatat ttccatgttt atggaaatat
     6001 caaaatcctc agcctgccat gctgcaactt ccgtgttgcg gaaaaccggt cgccgcctgc
     6061 cggcgaggca agccgcagcg acccgcgcgc gtactgcggc tttcccccgt catccgccgc
     6121 agccgcagtc ctcattcgtc ggcgcggcca gcccgcagac gcccgggtcg tcctggcagc
     6181 agtgttcggt caggtagccg agcagggcct gcatgcgcgc gaagtcggcg gcatagatca
     6241 gcgagcggcc gtcgcggcgc tgctcgacca gcccggcggc gcgcagcgtc ttcaggtgaa
     6301 acgacagtgt tgccggcgcc agtccgagcc gttcaccgat ctgtccggcc ggcaatccgg
     6361 gcggaccggc ctgcaccagc aggcggaaga tggcgaggcg gctgtcctgc gcgagggcgc
     6421 cgagcgcggc aacggcttcg gtctggtcca cggcggggtt ccggtgaggg gggatggagc
     6481 actgtagcgc gtgcgcgcga cgccacgagc cggtcgtcgg ctcgcccccg gagcccgctc
     6541 aggcttcctg agcgcgcgtg caagcgggtg tgcggcctcg caccgaggct gccgatgcag
     6601 gcggagcagc cggcacggac cgcgcaggcg ccccggcacg caccgatccc gatggtcgtc
     6661 cggatgccga tccggcgcgg atcccccgtc cgaccggccg ccaccgccga agccgtcccg
     6721 gcggcttgcg gatgacgcgg atggaagtgc cgatgacgcg catgggtgcg ccgatggcgc
     6781 gcagaaaagc gcagcagatg cgtgcctgcc gggctttcct gtgaaccgcc ccgctcaccc
     6841 cgtcagccgc gcatacagca gcggcagttc gcgcggcagt tcggcgggct tgcggatcag
     6901 cacccagccc tgcgtgccga acaggtacgg caggtagtcg tgggcctgct cgtcgatggt
     6961 cacgcagaac ggctgcaggc cggcctcgcg cgcctcgcgg atcgccgcgc gcgtgtcctc
     7021 gatgccccag cggccttcgt agcggtcgag gtcgttcggc ttgccgtcgg tgagcaggat
     7081 caggatgcgc cggctcgccg gttcgcggcc gagcagcgtc gtcgcgtggc ggatcgccgc
     7141 gcccatgcgc gtgtagtagc cggggcggat cgcggcgatg cgcccgcgga tgccggcgtc
     7201 gtggcgctcg tcgaaagcct tcagcgccag caggcgcacg tgatcgcgcc ggcgcgacga
     7261 gaagccgtag agcgcgtagc ggtcgccggt cgcggccagc gcctcggcga acaggtgcag
     7321 cgcgtcgcgg atcacgtcga tgacgcgacc gtgctcgccg acccaggcat cggtcgacag
     7381 cgacaggtcg gcgagcagca ggcaggcgag atcgcgcacg ccgccgcgga agtcgcggta
     7441 caggcgcacg tcgccgctcg ccgtaccgtt catgcgctcg gcgcggtgcg cgagccaggc
     7501 gtcgaggtcg atgtcggcgc catccggcag gccgcgcagc cagctgcgca ccgggcgcag
     7561 ctgctcgaac tgcgcgcgca gccgccgggc ggtgacgcgc aggtgcgccg gcagttcggc
     7621 cggcacggcg ccgcgcggca gcatcagctg cacgcggcag tgatcgggca gcagcacgcc
     7681 gcgcttccag tcccactccg gcaccgggat gccgtcgccg agcgggatgt cgtcctgccc
     7741 ggccgagggc aggtcgaggt cgaacctgag cttcgtcgcc ggcgccttgc cgcgcgcgac
     7801 gctcagcacg cgcaggtcat cggcgttgct gcaggcctgg tcgtcctcac tgtcgtcgtc
     7861 gcagcgatcg accttcacgt actcggtcca cgagaacatc gattcgagcc ggaaggccag
     7921 cagcccgcgc ttgccgtcgg gatcatcgac gcgctcggcc tgacgcttgc gcgcggcatc
     7981 gtccggcccg gtgtgaccgc tggcggcctc ggccggcgga tcggcgagcg gccgtgtcag
     8041 cttcgactgc gggcgcggcg gcgccggatg cagccacagc ggcaccggcg ccggcggccg
     8101 gcgcgcaccc ggcagcggca cggcagcggc gggatcggcg aggacggcgc ggatgctgcg
//
LOCUS       DAOQJH010000017         7909 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_4974_length_7909_cov_3.557805, whole genome shotgun sequence.
ACCESSION   DAOQJH010000017 DAOQJH010000000
VERSION     DAOQJH010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 7909)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7909)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7909
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4974_length_7909_cov_3.557805"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..911
                     /gene="earP"
                     /locus_tag="PLQ18_00820"
     CDS             <1..911
                     /gene="earP"
                     /locus_tag="PLQ18_00820"
                     /inference="COORDINATES: protein motif:HMM:NF021598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor P maturation arginine
                     rhamnosyltransferase EarP"
                     /protein_id="HNE00217.1"
                     /translation="ESLEAAECADAGDVVVEAFACELPAPARAALQARRSLWLNLEYL
                     SAEAWVEGCHGLGSAQPGGRTRYFFFPGFTPATGGLIGADAACAQWDERAARAWLAAQ
                     GVTGPAAARRLSLFTYGNAALPGWLEALAAGDEPWLALVPDGRTLRALGDWLGAPLAP
                     GETRTRAALSIHALPFLAQDDYDRLLWSSHLNVVRGEDSFVRAHWARRPLLWQAYRQD
                     GEAHLDKLEAWLARALDGLEAGAAGAARAAHRAWNREADFAAHWPALRAALPALEVQA
                     GRFAGRLAEGPELAAALVAFAQSKIE"
     gene            946..1509
                     /gene="efp"
                     /locus_tag="PLQ18_00825"
     CDS             946..1509
                     /gene="efp"
                     /locus_tag="PLQ18_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013835393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HNE00218.1"
                     /translation="MKTAQEIRAGAVILVDGQPMVVQKAEFNKSGRNAAVVKMKLKNL
                     LSGSGTETVYKADEKFDVVILDKKECTYSYYADPMFVFMDAEYNQYEIERDDLGDAVH
                     YIVDGMEDVCEVTFYEGKVISMELPTIVVREVEYTEPAARGDTSGKVMKLARLKGSGY
                     ELQVPAFVETGEKIEIDTRTSEFRKRA"
     gene            complement(1590..2516)
                     /locus_tag="PLQ18_00830"
     CDS             complement(1590..2516)
                     /locus_tag="PLQ18_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018936323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE00219.1"
                     /translation="MRASSTLEGRFAQIHVDAARNATDDFNPFHDPHHWQRIVANPFP
                     GPLVLGFQLEALIEYLWQTHPARDEDAAWAAAHGLHWTNWDLSFAGALCPGEAFTAEV
                     RAAARRETPEPGLSRRLLIRKADSLVLAGWLRQSAEALYEPGLSCAPFPGPLRAQPDR
                     CQRGPWFLKRKFASVGHAKNFLAGALADQALWFDELAPRSVFPQTYAAALLSCALLEQ
                     ATAAGHDFCAEPLVYTAHHLSILAPAVAGLRSNDALHLLVHGPELLAAGSGLGIGIEQ
                     TRYAGLVYAGTPERLLLRGELLLAPLSAITGG"
     gene            2589..3140
                     /locus_tag="PLQ18_00835"
     CDS             2589..3140
                     /locus_tag="PLQ18_00835"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HNE00220.1"
                     /translation="MREIEAVGVDVIIVFGAALAADGSPGPALLRRIEHAVALWQAGR
                     GRWLLCSGGLVAGTRSEAAVMAGCARAAGVPVQCIVEEDCARNTWQNAQFSAALLRRH
                     GWRTALLVTERYHLPRARLACRLNGLPVVAVSGPGHPPGRGRGRRLALALREGLACGM
                     MLMRAAAAILRRNGAESRQAAGD"
     gene            3507..4601
                     /locus_tag="PLQ18_00840"
     CDS             3507..4601
                     /locus_tag="PLQ18_00840"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HNE00221.1"
                     /translation="MPAQNIVPNTVATSAAHIEPCHGYAFDGDLVRLYAQIAVDAVPV
                     ANAEWAVQLWACATPFAGGELHGTKIAEVRAAVAAEAPAWLDATTTALPPAGAGEHAM
                     VLVLASGDDGHFAAIHDYANYPHAERFVLPRLGGTVGYRIDGGSVTLEVGSVANPRPA
                     DNLSGSLALEFWALAAPYAGADAFAGSKLAGVDLGTLAGGCALDGLTFTLSCAGQPDG
                     VWYPVLMLREWTAAGWVTRDYTAFAPSGTAAPLAAAPVAQAPAVETAPLAEAPVIEAP
                     AAAPAVVEPAPAVKPAKAKARKAKAAASGPAVSVNTSTVTALAEVKGISKTVAAAIVA
                     ARPYASLDDLLRAKGVGAKLLEKLRAQLTL"
     gene            complement(4694..5113)
                     /locus_tag="PLQ18_00845"
     CDS             complement(4694..5113)
                     /locus_tag="PLQ18_00845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE00222.1"
                     /translation="MSLSMSWFLALLVAALSAWMIRVFVRLPPRVERTRTPAPAAGRA
                     QPWQAVRIVPGAGACAAAHAQAARLYPPGHAPLMPLADGGCEHCHCQYETIQDRRRGE
                     RRSGMDRRTGLRYEPDRSDRRASRERRRTFGWSGSPI"
     gene            complement(5350..6081)
                     /locus_tag="PLQ18_00850"
     CDS             complement(5350..6081)
                     /locus_tag="PLQ18_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007437431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNE00223.1"
                     /translation="MTDNADTPVVLVTGASRGIGLAIVQAFAGAGWRTAGCARTPSPA
                     LAAAADLVQGCDVSDGAAVHALVEAVLGRFGRLDALVCNAGLAGSNPVGSEDDDARWR
                     AILATNLDGTWHACRAALPRLPDGHGRIVNIASVLALRGVADQPAYCAAKHGVLGLTR
                     SLALQAAARGITVNAICPGWTRTAMAEARYAELGIDAAQAAAGVPLGRIVEPAEVAAL
                     ALWLCSPAAGAITGQALTIDGGTLA"
     gene            complement(6123..6869)
                     /locus_tag="PLQ18_00855"
     CDS             complement(6123..6869)
                     /locus_tag="PLQ18_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017715059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNE00224.1"
                     /translation="MPNPPPVPCLPGLAGKVAIVTGHRTGIGAATAALLEGLGVQVHG
                     FDLPEVDLADLESIAGHVETVAARAGRLDLLVNNAGVTLIGSVTDTPLAEARRVLDVN
                     LLAPFLLMQAVIPHLIAAGGGAIVNVASDQAFVGKKGSAIYGASKAALAQLARSAALD
                     WAAHGIRVNAIAPGSTDTPMLRQVFADLRRRYPEAFPVEGAEAYVGAIPLGRLAEPRE
                     IAWTIAFLASDAASFITGTVLAVDGGFTAA"
     gene            7050..7574
                     /locus_tag="PLQ18_00860"
     CDS             7050..7574
                     /locus_tag="PLQ18_00860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE00225.1"
                     /translation="MTTPCDSRTCAPAGACVASCRQRLRRGVWLAGCLLVLGGCVEPA
                     YYGGSPYYEERNSYYESRPVYTQPDYYGGSYYRPAPRVQAYYYDRGYPHARGGRDWHD
                     HDRDRRGRDYRDDDRRSRNDGRGDEHREARRDQDRSRDSGDHRASSGRRTDLEWTDRM
                     RRAQGGNGETRPYD"
     gene            7567..7881
                     /locus_tag="PLQ18_00865"
     CDS             7567..7881
                     /locus_tag="PLQ18_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012356875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE00226.1"
                     /translation="MTDAVRQPEVRRPALVRWGLAGILLALTGDCVAVAPDYGYGYGY
                     APAYSPAPPAYSYAPAPVVVAPPVVVRPYYRPYYRDYRPYPRPYYRPYHNHGWGHGHR
                     RY"
BASE COUNT         1126 a         2723 c         2912 g         1148 t
ORIGIN      
        1 gggaatctct ggaagcggcc gagtgcgccg atgccggcga cgtggtcgtc gaagccttcg
       61 cctgcgaact gccggcgccc gcgcgtgccg cgctgcaggc gcgccggtcg ctgtggctga
      121 acctcgaata cctgtcggcc gaagcctggg tcgagggctg ccacgggctc ggcagtgcgc
      181 agccgggcgg gcggacgcgc tacttcttct ttcccggctt cacgccggcg accggcgggc
      241 tgatcggcgc cgatgcggcg tgcgcgcagt gggatgagcg ggccgcgcgc gcgtggctgg
      301 ccgcgcaggg cgtgacgggg ccggcggcgg cgcggcggct gtcgctgttc acgtacggca
      361 atgccgcgct gccgggctgg ctggaggcgc tcgcggcggg cgatgagccg tggctcgcgc
      421 tggtgccgga cgggcgcacg ctgcgcgcgc tcggcgactg gctcggggcg ccgcttgcgc
      481 ccggcgaaac gcgcacgcgc gctgcgctga gcatccacgc gctgcccttc ctcgcgcagg
      541 acgactacga ccggctgctg tggagcagcc atctcaacgt cgtgcgcggc gaggacagct
      601 tcgtgcgcgc gcactgggcg cgccggccgc tgctgtggca ggcctaccgg caggacgggg
      661 aggcgcacct cgacaagctc gaagcctggc tcgcgcgggc gctggacggg ctggaggccg
      721 gggccgccgg cgctgcgcgc gcagcgcacc gggcctggaa ccgggaggcg gatttcgcgg
      781 cacactggcc ggcgctgcgc gcggcgctgc cggcgctgga ggtgcaggcc gggcgttttg
      841 ccgggcggct ggcggaaggg ccggagcttg cggccgcgct ggtggcgttc gcgcaatcga
      901 agatagaata agcagttttt acacccaacc caccaggatc acccgatgaa aaccgctcag
      961 gaaatccgcg ccggcgccgt cattctggtc gacggtcagc cgatggtcgt gcagaaggct
     1021 gagttcaaca agtccggccg caacgccgcg gtcgtgaaga tgaagctgaa gaacctgctg
     1081 tcgggttccg gcaccgagac ggtctacaag gccgacgaga agttcgatgt cgtcatcctc
     1141 gacaagaagg aatgcacgta ctcgtactac gccgatccga tgttcgtgtt catggacgcc
     1201 gaatacaacc agtacgagat cgagcgcgac gatctcggcg atgccgtcca ctacatcgtc
     1261 gacggcatgg aagatgtctg cgaagtgacc ttctacgaag gcaaggtcat ctcgatggaa
     1321 ctgccgacca tcgtcgtgcg cgaggtcgaa tacaccgagc cggccgcacg cggtgacacc
     1381 tcgggcaagg tcatgaagct cgcgcgcctc aagggcagcg gctacgaact gcaggtgccg
     1441 gccttcgtcg agaccggcga gaagatcgaa atcgacacgc gcaccagcga gttccgcaag
     1501 cgcgcgtgaa gccggtcacc cccgccggat gtgtccgccc tcgcccccgg tcgccgtccg
     1561 cgatcggggg cgatgcgtct gggcagccgc tatccgccgg tgatcgccga cagcggcgcg
     1621 agcagcagtt cgccacgcag cagcaggcgc tccggcgtgc cggcgtagac gaggccggcg
     1681 tagcgcgtct gctcgatgcc gatgccgagc ccgctgccgg ccgcaagcag ctcggggccg
     1741 tgcacgagca ggtgcagcgc atcgttgctg cgcagcccgg ccacggccgg cgcgaggatg
     1801 ctcaggtgat gcgcggtata cacgagcggt tcggcgcaga agtcatgccc ggccgccgtc
     1861 gcctgttcga gcagcgcaca cgagagcagc gccgcggcgt aggtctgcgg gaacaccgag
     1921 cgcggtgcca gttcgtcgaa ccacagcgcc tggtcggcca gcgcgccggc gaggaagttc
     1981 ttggcgtggc cgacgctcgc gaacttgcgc ttcaggaacc aggggccgcg ctggcagcgg
     2041 tcgggctgcg cgcgcagcgg cccggggaag ggcgcgcagc tcaggcccgg ctcgtacagc
     2101 gcttcggcgc tctggcgcag ccagccggcc agcaccaggc tgtcggcctt gcggatcagc
     2161 agccggcgcg acagccccgg ctccggagtc tcgcgccggg ccgccgcgcg cacttcggcc
     2221 gtgaacgcct cgcccggaca cagcgcgccg gcgaaggaca gatcccagtt cgtccagtgc
     2281 aggccgtgcg ccgcggccca ggccgcgtcc tcgtcacggg ccggatgggt ctgccacaga
     2341 tactcgatca gggcttcgag ctggaagccg agcaccagcg gacccgggaa cgggttggcg
     2401 acgatgcgct gccagtggtg cggatcgtgg aagggattga agtcgtcggt ggcattgcgc
     2461 gcggcatcca cgtggatctg ggcaaaccgg ccttcgagcg tcgagctggc gcgcatcgcg
     2521 gactccgggc ctgcgccgcg ccggcggact gccggtgcgc atgcggccgt cgccgagctt
     2581 agtcgcgcat gcgtgagatc gaagccgtgg gagtcgacgt catcatcgtg ttcggtgcgg
     2641 cgctggctgc ggacggttcg ccggggccgg cgctgctgcg gcgcatcgaa cacgccgtcg
     2701 cgctgtggca ggccgggcgc gggcgctggc tgctgtgctc gggcggactc gttgccggca
     2761 cgcgcagcga agccgcggtg atggccggat gcgcgcgcgc tgccggagtg ccggtgcagt
     2821 gcatcgtcga ggaggactgc gcgcgcaaca cctggcagaa cgcgcagttc agcgcagcac
     2881 tgctgcgcag gcatggctgg cgcacggcgc tgctggtgac cgagcgctat cacctgccgc
     2941 gcgcccggct cgcctgccgg ctgaacgggc tgccggtcgt tgccgtcagc ggtccggggc
     3001 atccgcccgg acgcggccgg ggccggcgcc tcgccctggc gctgcgtgag ggactcgcct
     3061 gcgggatgat gctcatgcgg gctgccgcgg ccattctgcg ccgtaacggt gcagaaagcc
     3121 ggcaggccgc cggcgattga acgggtgatc gtcccgacac cggtactgcg catgacgatt
     3181 gccggcaatc tcccgggcgc cggcagccgg tcgacaccgg cggtttcggc aattgcacca
     3241 ggttggcgca aatcagcatg agcgctggcc cgggcatttc cggtacggac aaatccggtt
     3301 tgtccgcacc ggtcccggca ataccggtgc actgcgtcat gaccggtgtg ccggtgtcca
     3361 gcctgctgat ggtaaagggg aaaaattacc gcgccgtgtc cgggaattgc cggccaatag
     3421 ccggcatccg gaagcgcgtg cattttcatc ggaagcagaa tctgtataat cccccgccct
     3481 ttccgggtat tgggaacatt gccgaaatgc cagctcagaa tatcgttccg aacaccgtcg
     3541 ccacgtctgc cgcccatatc gaaccctgcc acggttacgc cttcgatggt gatctggtgc
     3601 gtctgtacgc gcagatcgcc gtcgatgccg tgccggtggc caatgccgaa tgggccgtgc
     3661 agctctgggc ctgcgcgacg ccgtttgccg gcggtgaact gcacgggacc aagatcgccg
     3721 aggtgcgcgc cgcggtggcg gccgaggcgc cggcctggct cgatgcgacc acgacggcgc
     3781 tgccgccggc cggggccggc gagcatgcga tggtgctcgt gctcgccagc ggcgacgacg
     3841 gccacttcgc ggcgatccac gactatgcga actatccgca cgccgaacgc ttcgtgctgc
     3901 cgcgcctcgg cggcacggtc ggctaccgga tcgacggcgg cagcgtcacg ctcgaagtcg
     3961 gatcggtcgc caacccgcgc ccggccgaca acctcagcgg ttcgctcgcg ctcgagttct
     4021 gggcgctcgc cgcgccgtat gccggtgccg atgcgttcgc cggcagcaag ctcgccggcg
     4081 tcgatctcgg cacgctggcc ggtggctgcg cgctcgacgg cctgaccttc acgctgagct
     4141 gtgccggcca gccggacggc gtctggtacc cggtgctgat gctgcgcgag tggacggccg
     4201 ccggctgggt cacgcgcgac tacaccgcct tcgcgccctc gggcactgcc gcgccgctgg
     4261 ccgccgcccc ggtggcgcaa gccccggccg tcgaaaccgc tccgcttgcc gaagcgccgg
     4321 tcatcgaggc gccggccgcc gcgcctgcgg tcgtcgagcc tgcaccggcc gtgaagccgg
     4381 ccaaggccaa ggccaggaag gcaaaggccg cggcgtcggg accggcggtg tcggtgaaca
     4441 cctcgacggt gaccgcgctg gccgaggtca agggcatttc gaagaccgtc gctgccgcca
     4501 tcgtcgccgc ccgcccgtat gcgagcctcg acgatctgct gcgggccaag ggcgttggcg
     4561 ccaagctgct cgaaaagctg cgtgcgcagc tgacgctgta agccggccgc gtctcatgcg
     4621 gccggtggtc gcccgctgca cgggcggcgg cgcggcggcg ggattcgtcc cgggatcaga
     4681 tcaggtcgcg ggatcagatg ggggagccgc tccacccgaa tgtgcgccgg cgctcgcggc
     4741 tggcgcgccg gtccgagcga tccggctcgt agcgcaggcc ggtgcgccgg tccatgccgc
     4801 tgcgccgttc gccgcggcgc cggtcctgga tggtttcgta ctggcaatgg cagtgctcgc
     4861 agccgccatc ggcgagcggc atcagcggtg cgtggccggg cgggtacagc cgggcggcct
     4921 gcgcgtgggc cgccgcacag gcgcccgcgc cgggcacgat gcggaccgcc tgccagggct
     4981 gcgcgcgacc ggccgcgggt gcgggcgtgc gcgtgcgttc gacgcgcggc ggcagacgca
     5041 cgaacacccg gatcatccag gccgacaggg cagccaccag cagggcgaga aaccaggaca
     5101 tggacaggga catcgtcgga acccgcgcgc cggcgcgagt ccgccggaac gtcttgtctg
     5161 gacgatcacc ggccgggctg tccggtcggc accggcctcc tggcaggtcc gctccgggac
     5221 cgatccacgg cacaccggat atcgcgctgg agcgcgccgg cgacctccgc tctgccgccg
     5281 gaggattttt cgtaaagttt agtcacatga tcgtccggat ccgtccggac ttgagcgccg
     5341 tcgacggctt caggcgagcg tgccgccgtc gatggtcagc gcctggccgg tgatggcgcc
     5401 ggccgccggc gagcacagcc agagcgccag cgccgcgacc tccgcgggct cgacgatgcg
     5461 cccgagcggc acgccggcgg cggcctgtgc ggcgtcgatg ccgagttcgg cgtagcgggc
     5521 ctcggccatc gccgtgcgcg tccagcccgg acagatggcg ttgacggtga tgccgcgcgc
     5581 cgccgcctgc agcgccagcg agcgcgtcag cccgagcacg ccgtgcttgg ccgcgcagta
     5641 ggccggctgg tccgccacgc cgcgcagtgc cagcaccgag gcgatgttga cgatgcggcc
     5701 gtgtccatcg ggcagccgcg gcagcgcggc gcggcaggcg tgccaggtgc cgtcgaggtt
     5761 ggtcgcgagg atggcgcgcc agcgcgcatc gtcgtcctcg gagccgaccg gattgctgcc
     5821 ggcgaggccg gcattgcaga ccagcgcatc gagccggccg aagcgcccga gcaccgcctc
     5881 gaccagcgcg tgcacggccg cgccgtcgct cacgtcgcag ccctgcacca gatcggcggc
     5941 tgcggccagt gccgggcttg gcgtgcgcgc gcagccggcc gtgcgccagc cggcgccggc
     6001 aaaggcctgc acgatcgcca gcccgatgcc gcgtgaggcg ccggtgacca gtacgaccgg
     6061 ggtgtctgca ttgtctgtca tcgtatggtt catcttgtca tcgaatcagc cgctgatccg
     6121 gttcaggcgg ccgtgaaacc gccgtcgacc gccagcacgg taccggtgat gaacgaggcg
     6181 gcgtccgagg cgagaaacgc gatcgtccac gcgatctcgc gcggctcggc cagccgcccg
     6241 agcgggatcg cgccgacgta ggcctcggcg ccctcgacgg ggaaggcctc cgggtagcgc
     6301 cggcgcaggt cggcgaacac ctgacgcagc atcggcgtgt cggtgctgcc gggggcgatg
     6361 gcgttgacgc ggatgccgtg cgcagcccag tcgagcgcgg cgctgcgcgc aagctgtgcg
     6421 agcgcggcct tgctggcgcc gtagatcgcc gagcccttct tgccgacgaa ggcctgatcg
     6481 ctggcgacgt tgacgatcgc gccgcctccg gccgcgatca ggtgcggaat caccgcctgc
     6541 atcagcagga agggggcgag caggttcacg tcgagcacgc gccgcgcttc ggcgagcggc
     6601 gtgtcggtga cgctgccgat cagcgtcacg ccggcattgt tgacgagcag atcgagccgg
     6661 ccggcgcgcg cggccacggt ctcgacgtgt ccggcgatcg attccagatc ggcgagatcg
     6721 acctcgggca ggtcgaagcc gtgcacctgc acgccgagcc cttcgagcag cgcggcggtc
     6781 gcagcgccga tgccggtgcg atgaccggtg acgatcgcca ccttgccggc aagaccgggc
     6841 aggcagggga cgggcggggg attcggcatg cgggcaggct ccgggcgcgg ggcaggggcg
     6901 cgagtatgtc gcaagcggtg acgggccgct gccgctggcc ctgctgcgcc atactgaccg
     6961 tctgacgcac gcaaccaccg gggcgtgtgc tgtgtctgaa tcctggccgg tgttctccct
     7021 tccgtgcccg gcgctgccga ggttcgatca tgaccactcc ctgcgattcc cggacgtgcg
     7081 cgccagccgg cgcgtgcgtt gcttcgtgtc gccagcgcct gcggcgcggc gtctggctgg
     7141 ccggctgcct gctcgtgctc ggcggctgcg tcgagccggc ctactacggc ggctcgccgt
     7201 attacgaaga aagaaattcg tattatgaaa gcaggccggt gtacacgcag ccggattact
     7261 acggcggcag ctattaccgg ccggctcccc gggtccaggc gtattactac gaccgcggat
     7321 acccgcatgc gcgggggggg cgcgactggc atgaccacga ccgtgaccgg cgcggtcgcg
     7381 attaccgcga tgatgaccgg cgctcgcgca atgacggtcg cggcgatgag catcgcgaag
     7441 cccgccgcga tcaggaccgg tcccgggatt ccggcgatca tcgggcctct tccggccggc
     7501 gtaccgattt ggaatggacg gaccgcatgc gcagggccca gggtggtaac ggtgaaacac
     7561 gtccttatga ctgatgccgt ccggcagcct gaagtccgga ggccggccct tgtccgctgg
     7621 gggcttgcag gcatcctgct ggccctgaca ggcgactgcg tggcggtcgc gcccgattac
     7681 ggctacggct acggctacgc cccggcctat tcgccggcgc cgccggccta cagctacgcc
     7741 cccgcgcccg tggtcgtcgc gccgccggtg gtcgtgcggc cgtactaccg gccttattac
     7801 cgcgattacc ggccttaccc tcgcccttat tatcggcctt atcacaatca cggctggggg
     7861 catggccatc gccgctactg agcgcccggc cctccggggc cggacgggc
//
LOCUS       DAOQJH010000018         7830 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_5059_length_7830_cov_4.201543, whole genome shotgun sequence.
ACCESSION   DAOQJH010000018 DAOQJH010000000
VERSION     DAOQJH010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 7830)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7830)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7830
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5059_length_7830_cov_4.201543"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            336..1262
                     /locus_tag="PLQ18_00870"
     CDS             336..1262
                     /locus_tag="PLQ18_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015918602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE00227.1"
                     /translation="MGYARWDSDDWSDYAAGQAGRSREEVFTRRSLDPEFDPLNIRRR
                     ESRDSAQNPESNAIIVAFDETGSMGSIPDAFVREGLGTMVQALLDRRPVSDPHVMVMG
                     CGDAWCDRAPLQVTQFEADLRIADQLSRLWLEGGGGGNRFESYNLPWYFAARRTSIDC
                     FEKRGRKGYLFTVGDEPPPPKLLAAHVRAFLGDDLRRDLDNAELLRAAGRMYEIFHVV
                     VEQGSHYRSFPDEVEQGWTRLLGQRVLFLADYRQLAEVIVSAIEVNEGRDPDAVVASW
                     DAAAGTVAKALRRLPAASRRRGLLGYFGRSAR"
     gene            1262..2449
                     /locus_tag="PLQ18_00875"
     CDS             1262..2449
                     /locus_tag="PLQ18_00875"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003589773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HNE00228.1"
                     /translation="MRALAVIGAGYGDEGKGAATDRLAATLGPRALVVRHNGGAQAGH
                     TVIAPDGRRHVFGHIGSGSFAGVPSYLSEYFVASPILCLRELRALAVLGVQPKLHVDA
                     GCAVTTPYDMLLNQAVEAARGAGRHGSCGVGFGETIERHGHAGFALTVADLRKPVRLR
                     ERLEAIRRDWLPARAAALGLDRLPAVAYDNALSVRFVDDCARFLAAIEVCDAGVLRGR
                     PLIFEGAQGLGLDMDRGAFPHVTRSHTGIRNAVTLTRAAGLDALQVQYATRAYATRHG
                     AGPLAHELPAAPAAAVRDATNRPNDWQGRLRFGWLDLDRLATDIRTDLADAGALAVTA
                     ELIVSCLDQMDEVIPHVADGGLVQVPRERCLAWYAQRLGLRAGYGAYGPGRDAVREWA
                     RAA"
     gene            complement(2582..2851)
                     /locus_tag="PLQ18_00880"
     CDS             complement(2582..2851)
                     /locus_tag="PLQ18_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012315648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HNE00229.1"
                     /translation="MSASHTKPPSATDWARLQAMTDDDIDFSDDPERDAHFLAQAVIW
                     PGPKKQITLRIDADVLGYFREQGRGYQTLINRVLRKYVEAQKNRA"
     gene            complement(2781..3131)
                     /locus_tag="PLQ18_00885"
     CDS             complement(2781..3131)
                     /locus_tag="PLQ18_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015722121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HNE00230.1"
                     /translation="MSDRYQEHGFDDHVEWAWDEQKNASNIEKHGIDFQDLPRVTANP
                     ILIARDTRRDYGEIRWQAIGRIQGITIAFVFTEREPRIVRIISARKASKNERIAYETA
                     IRDRLGPSAGDDGR"
     gene            complement(3377..5599)
                     /gene="paoC"
                     /locus_tag="PLQ18_00890"
     CDS             complement(3377..5599)
                     /gene="paoC"
                     /locus_tag="PLQ18_00890"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020188096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047770 - carboxylate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde oxidoreductase molybdenum-binding
                     subunit PaoC"
                     /protein_id="HNE00231.1"
                     /translation="MKFDTPSAENPTDRLRVVGQPVDRIDGPLKTTGRAPYAYEHHDG
                     FTGGFADGLARPAYGHVLGAGIARGRIRSFDAEAARRAPGVLAVISADNAGPLGQGPR
                     NTAKLLGGPRIEHYHQAIALVVAESFEQARAAARLIRVEYAREAGEFDLAKVRDSGRA
                     PQDEPDRRLGDFAAAFAASAVRIDAVYRTPDQSHAMMEPHASMAAWDGDRLTLWTSNQ
                     MIAWGRADLAKTLGIRPEQIRLLSPYIGGGFGGKLFVRADAVLAALGARAIGRPVKVM
                     LARPLVFNNTTHRPATIQRIRIGATADGHIHAIAHESWCGNLPGGSAETATQQTQLLY
                     AGAHRLSALRLATLDLPEGNAMRAPGEAPGLMALEIAIDELAEKLGLDPVELRIRNDT
                     PVDPHDPQRRFSQRQLVECLRTGAARFGWAQRNPVPGRTRAGRWLLGHGVAAAFRNNL
                     VMKSGARVRLGRDARVTVETDMTDIGTGSYTVIAQTAAEMLGVPLAQVDVRLGDSDFP
                     VSAGSGGQWGGNCSTAGVYAACMRLREQIAQRLGFRDAAAVRFADGEVQAGGRVAPLA
                     DAVRDGELLAEDSIEFAGLDRKYQQSTFGAHFVEVGVDAATGETRVRRMLAVCAAGRI
                     LNPKTARSQVIGAMTMGVGAALSEALHVDPRLGCFVNHDLAGYEVPVHADIPHQEVIF
                     LDGADPVSSPMKAKGVGELGLCGVAAAIANAVYNATGVRVRDYPLTLDKLLGQMPAPG
                     "
     gene            complement(5604..6554)
                     /locus_tag="PLQ18_00895"
     CDS             complement(5604..6554)
                     /locus_tag="PLQ18_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019462481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HNE00232.1"
                     /translation="MKPFTYERAGSPQAAVASAAAHPGARFIAGGTNLLDLMKLEIET
                     PAHLIDVNGLGLDQIEGTADGGLRIGALVRNSDLAADARVRRDYALLARALLSGASGQ
                     LRNRATTAGNLLQRTRCPYFYDSDLPCNKRLPGSGCAALGGYSRQHAILGVSEACIAA
                     HPSDMAVALRALDAVVETLRPDGSTRSLALADFHRLPGETPQVEHALEPGELIVAVTL
                     PPPPGGRHVYRKVRDRASYAFALVAVAVIVQPDGRGRVALGGVAPKPWRLEAAEAELP
                     RGASAVATALLAEARTTRENAYKTLLVERTLAAALAEARS"
     gene            complement(6551..7198)
                     /gene="paoA"
                     /locus_tag="PLQ18_00900"
     CDS             complement(6551..7198)
                     /gene="paoA"
                     /locus_tag="PLQ18_00900"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006220758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0047770 - carboxylate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase iron-sulfur subunit PaoA"
                     /protein_id="HNE00233.1"
                     /translation="MDDCLHLKITRRRLLIASAVSASAAALPSLSGAASRDAPQTAAV
                     PVQQRVRLRINGEVHSLELDTRTTLLDALREHLQLTGTKKGCDHGQCGACTVLLDGRR
                     INACLTLAVMHEGAELTTIEGLGTPGRLHPMQAAFVKHDAFQCGYCTPGQICSALGML
                     DELRAGQPSHVSADLEAAPTLSADEIRERMSGNLCRCGAYSNLVEAITEAAGSRA"
     gene            7412..7759
                     /locus_tag="PLQ18_00905"
     CDS             7412..7759
                     /locus_tag="PLQ18_00905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE00234.1"
                     /translation="MHESSIGTAPAVTGPATPGAGGADAGGLQSKTGACATRNQPVIA
                     ALAGFRTVCPDKFNNQSNPADMMIFITVLTDKLSILLLWTELMSASPASRHMRREHSP
                     LPFFARAQDCAAG"
BASE COUNT         1116 a         2694 c         2816 g         1204 t
ORIGIN      
        1 gcggtatcgg catcctcatc gacgtgcacg tagccgccgg gcagcgccgg ccggccgcgg
       61 aacggctcgc acgcgcgctc ggcgatcagc acctgcagac cgtcgtcatt ggcgggttct
      121 gccagcgtga acagcacgat gtcgacggtg gcgatcgggc ggaggtgttc gctcatctcg
      181 gggcttccgg cgcgtcgtgg cggctgcagg cagggggcgg cggcgcgcag cctaaaaggt
      241 cattcctatt tcttgcaaat ggaaacttgc aatttgcatt tatgtggatc agtcttggtg
      301 ccgtcaccaa cgaccgccac gagaggagcg ccgccatggg ttacgcccgc tgggattccg
      361 atgactggag cgattacgcc gccggccagg ccggccgcag ccgcgaggaa gtgttcacgc
      421 gccgcagcct cgatcccgag ttcgatccgc tgaacatccg ccggcgcgag tcgcgcgatt
      481 cggcgcagaa ccccgaatcg aacgcgatca tcgtcgcctt cgacgaaacc ggctcgatgg
      541 gcagcattcc cgatgccttc gtgcgcgagg gcctcggcac gatggtgcag gcgctgctcg
      601 accgccggcc ggtcagcgat ccgcacgtga tggtcatggg ctgcggcgac gcctggtgcg
      661 accgcgcgcc gctgcaggtc acgcagttcg aggccgacct gcgcatcgcc gatcagctct
      721 cgcggctgtg gctcgaaggc ggcggcggcg gcaaccgctt cgagagctac aacctgccgt
      781 ggtacttcgc ggcgcggcgc acgtcgatcg actgcttcga gaagcgcggc aggaagggct
      841 atctgttcac ggtcggcgac gagccgccgc cgccgaagct gctcgccgcg cacgtgcgcg
      901 ccttcctcgg cgatgacctg cgccgcgacc tcgacaacgc cgaactgctg cgcgccgccg
      961 ggcgcatgta cgagatcttc cacgtcgtcg tcgagcaggg cagccactac cgcagctttc
     1021 cggacgaggt cgaacagggc tggacgcggc tgctcggcca gcgcgtgctg ttcctcgccg
     1081 actaccgcca gctcgccgaa gtcatcgtct cggcgatcga agtcaacgag ggccgcgacc
     1141 cggatgccgt ggtcgcgagc tgggacgccg ccgccggcac agtcgcgaag gccttgcgcc
     1201 ggctgccggc agcgtcgcgc cggcgcggcc tgctcggcta cttcggccgg agcgcccgct
     1261 gatgcgcgcg ctcgcggtga tcggcgccgg ctacggcgat gagggcaagg gcgcggccac
     1321 cgaccggctg gccgcaaccc tcgggccgcg ggcgctggtc gtgcgccaca acggcggtgc
     1381 gcaggccggc cacaccgtca tcgcgcccga tggccgccgg cacgtgttcg gccacatcgg
     1441 cagcggcagt ttcgccggcg tgccgagcta cctgtcggag tacttcgtcg ccagcccgat
     1501 cctgtgcctg cgcgaactga gggcgcttgc cgtactcggc gtgcagccga agctgcacgt
     1561 cgatgccggc tgcgcggtga cgacgccgta cgacatgctg ctgaaccagg ccgtcgaggc
     1621 cgcacgcgga gccggccggc acggctcctg cggcgtcggc ttcggcgaga cgatcgaacg
     1681 ccacggccat gccggtttcg cgctcacggt cgccgacctg cgcaaacccg tgcggctgcg
     1741 cgaacggctc gaagcgatcc gccgcgactg gctgcccgcg cgcgccgcgg cgctcggtct
     1801 cgaccggctg ccagcggtgg cttacgacaa tgcgctgtcg gtgcgcttcg tcgacgactg
     1861 cgctcgcttc ctggccgcaa tcgaggtctg cgatgccggc gtgctgcgcg gccgaccgct
     1921 gatcttcgag ggcgcgcagg ggctcggcct cgacatggac cgcggcgcct tcccgcacgt
     1981 cacgcgctcg cacaccggca tccgcaacgc cgtgacgctg actcgcgcgg ccggcctcga
     2041 cgcgctgcag gtgcagtacg ccacccgcgc ctacgccacc cgccacggcg ccggcccgct
     2101 cgcgcacgaa ctgccggccg caccggccgc ggcggtgcgc gatgcgacca accgcccgaa
     2161 cgactggcag ggccggctgc gcttcggctg gctcgacctc gaccgcctcg ccaccgacat
     2221 ccggaccgac ctcgccgatg ccggcgcgct ggcggtgacg gcggagctga tcgtcagctg
     2281 cctcgaccag atggacgagg tgatcccgca cgtcgcggac ggcggactcg tgcaggtgcc
     2341 gcgcgaacgc tgcctggcgt ggtacgcgca gcggctggga ctgagggccg gttacggcgc
     2401 gtacggaccg gggcgggatg cggtgcggga atgggcgcgg gcggcgtgag gccattgcag
     2461 cgcgactgcc ggcgcaggcc gtcccgggca gcgcggtcat tcgcgaaacg gaccgcgcgc
     2521 cgggttccct cctcaacgcc ggcgatcagg gcatgcaggc agccgcgggg cgcagggccc
     2581 atcaggcacg attcttctgc gcctcgacgt atttcctgag cacgcggttg atgagtgtct
     2641 gatagcccct gccctgttcc cggaaataac cgaggacatc ggcatcgatt ctcagcgtga
     2701 tctgcttctt cggtcccggc cagatcacgg cctgcgccag aaaatgtgcg tcgcgttccg
     2761 gatcgtcgga gaagtcgatg tcatcgtccg tcatcgcctg cagacgggcc cagtcggtcg
     2821 cggatggcgg tttcgtatgc gatgcgctca ttcttgctgg ccttcctcgc agaaatgatt
     2881 ctgacgatgc ggggttcgcg ttccgtgaac acgaaggcga tcgtgatgcc ctgaatcctg
     2941 ccgatggcct gccagcggat ttctccatag tccctgcggg tgtcccgggc aatgaggatg
     3001 gggttggccg tcacccgcgg caggtcctgg aaatcaatgc cgtgcttttc gatgttgctg
     3061 gcgttcttct gctcatccca cgcccattcg acgtgatcgt cgaaaccatg ctcctgatag
     3121 cgatcggaca tcgcaggctc cgggcgccgg caggatgtgc cttcgcctga tcgatcgggc
     3181 gacagaagct tgaatccgtg gatcaccgca tggtgtaact acaaacgtag ttacactacg
     3241 cgcccacgtc aacagggcgg atgcgcacgt ggcgcacggc gtccctgaag ccggcggaca
     3301 gcttgcagga gggcggaaag cggcaagtcg cggtggcggc tgcgatggcc gcagcaccgg
     3361 gccgctgccg cgtggatcag cccggtgccg gcatctgccc gagcagcttg tcgagcgtca
     3421 gcgggtagtc gcgcacgcgc acgccggtgg cgttgtagac ggcgttggcg atggcggcgg
     3481 cgacgccgca caggccgagt tcgccgacgc ccttggcctt catcggcgag gacaccggat
     3541 cggccccgtc gaggaagatg acctcctggt gcgggatgtc ggcgtgcacc ggcacctcgt
     3601 agccggcaag gtcgtggttc acgaagcagc cgagccgcgg gtcgacatgc aaggcctcgc
     3661 tgagcgcggc gccgacgccc atcgtcatcg cgccgatcac ctggctgcgt gccgtcttcg
     3721 ggttcaggat gcgcccggcc gcgcatacgg ccagcatccg gcgcacgcgc gtctcgccgg
     3781 tcgcggcatc gacgccgacc tcgacgaagt gcgccccgaa ggtcgactgc tggtacttgc
     3841 ggtcgagacc ggcgaactcg atcgaatcct cggcgagcag ttcgccgtcg cgcacggcat
     3901 cggcgagggg cgcgacgcgc ccgccggcct gcacctcgcc gtccgcgaag cgcacggccg
     3961 cggcatccct gaagccgagc cgctgcgcga tctgctcgcg cagacgcatg caggcggcgt
     4021 agacgccggc ggtcgagcag ttgccgcccc actgcccgcc ggagccggcc gagaccggga
     4081 agtccgaatc cccgaggcgt acgtcgacct gcgcgagcgg cacgccgagc atctcggcgg
     4141 cagtctgggc gatgacggtg tagctgccgg tgccgatgtc ggtcatgtcg gtctcgaccg
     4201 tcacgcgtgc gtcgcgcccg agccgcacgc gcgcgccgga tttcatgacc aggttgttgc
     4261 gaaacgccgc ggcgacgccg tggccgagca gccagcggcc ggcgcgggtg cggccgggga
     4321 cgggattgcg ctgcgcccag ccgaagcgcg cggcaccggt gcgcaggcac tcgaccagct
     4381 ggcgctgcga gaagcgccgc tgcgggtcgt gcggatcgac cggcgtgtcg ttgcggatgc
     4441 gcagctcgac cggatcgagg ccgagctttt cggcaagctc gtcgatcgcg atctccagcg
     4501 ccatcagtcc cggtgcctcg cccggggcgc gcatcgcgtt gccttcgggc aggtcgagcg
     4561 tcgccaggcg cagcgcgctc aggcgatggg cgccggcata gagcagctgc gtctgctggg
     4621 tcgccgtctc ggcgctgccg cccggcaggt tgccgcacca gctctcgtgg gcgatcgcgt
     4681 ggatgtggcc gtctgcggtc gcgccgatgc ggatgcgctg gatcgtcgcc ggccggtgcg
     4741 tggtgttgtt gaagaccagc ggccgcgcaa gcatgacctt gaccggccgg ccgatcgcac
     4801 gcgccccgag cgcagcgagc acggcatcgg cgcgcacgaa cagcttgccg ccgaaaccgc
     4861 cgccgatgta cggcgacagc agccggatct gctccggcct gatgccgagc gtcttcgcga
     4921 gatcggcgcg gccccaggcg atcatctggt tcgaggtcca gagcgtcagc cgatcgccgt
     4981 cccaggccgc catcgatgcg tgcggctcca tcatcgcgtg cgactggtcg ggcgtgcggt
     5041 agacggcgtc gatgcgcacg gccgaggcgg cgaaggccgc ggcgaagtcg ccgagccggc
     5101 ggtcgggttc gtcctgcggc gcccggccgc tgtcgcgcac ctttgccaga tcgaactcgc
     5161 cggcctcgcg cgcgtattcg acgcggatca gccgcgccgc ggcgcgcgcc tgctcgaagc
     5221 tttcggcgac gaccagcgcg atggcctggt gatagtgctc gatccgcggc ccgccgagca
     5281 gcttcgcggt gttgcgcgga ccctggccga gcgggccggc gttgtcggcg ctgatcaccg
     5341 cgagcacgcc gggcgcgcgg cgcgctgctt cggcatcgaa cgagcggatg cggcccctgg
     5401 cgatgccggc accgagcaca tgaccgtacg cgggcctggc gagaccgtct gcgaagccgc
     5461 ccgtgaagcc gtcgtgatgc tcgtaggcat acggcgcacg gccggtggtc ttcagcgggc
     5521 cgtcgatgcg gtcgacgggc tggccgacga cacgcaggcg atcagtgggg ttttcagcgg
     5581 atggcgtgtc gaatttcatc ggctcagctc cgggcttcgg ccagcgccgc cgcgagcgtg
     5641 cgctcgacca gcagcgtctt gtaggcgttc tcgcgcgtgg tgcgtgcctc ggcgagcagc
     5701 gcggtggcga cggcggacgc accacgcggc agttcggcct cggctgcttc gaggcgccac
     5761 ggcttcggcg cgacgccgcc gagcgcgacg cgcccgcgac cgtcgggctg gacgatgacg
     5821 gcgaccgcga ccagcgcgaa cgcgtacgag gcgcggtcgc ggaccttgcg gtacacgtgc
     5881 cggccaccgg gcggcggcgg cagcgtcacg gcgacgatca actcgccggg ctccagcgcg
     5941 tgctcgacct gcggcgtctc gccgggcagg cgatggaagt cggccagcgc gaggctgcgc
     6001 gtgctgccgt ccgggcgcag cgtctcgacc acggcgtcga gcgcgcgcag cgccacggcc
     6061 atgtcgctcg gatgcgcggc gatgcaggcc tcgctgacgc cgaggatcgc gtgctggcgg
     6121 ctgtagccgc cgagtgccgc gcagccgctg ccgggcaggc gtttgttgca cggcaggtcg
     6181 ctgtcgtaga agtacgggca gcgcgtgcgc tgcagcaggt tgccggcggt ggtcgcacgg
     6241 ttgcgcagct gccccgaggc gccggagagc agcgcgcgcg caagcagcgc gtagtcgcgg
     6301 cgcacgcgcg catcggcggc gagatcgctg ttgcgcacca gcgcaccgat gcgcaggcca
     6361 ccgtcggcgg tgccctcgat ctgatcgaga ccgaggccgt tcacgtcgat caggtgcgcc
     6421 ggcgtctcga tctccagctt catcagatcg agcaggttgg tgccgccggc gatgaagcgc
     6481 gcgcccggat gcgcggcggc actggcgacc gctgcctgcg gcgagccggc gcgttcatac
     6541 gtgaacggtt tcatgcgcgg ctcccggcag cttcggtgat cgcttcgacg aggttcgagt
     6601 aggcgccgca gcggcagagg ttgccgctca tgcgctcgcg gatttcgtcg gcgctcagtg
     6661 tcggtgcggc ttcgagatcg gcgctgacgt ggctcggctg gccggcacgc agttcatcga
     6721 gcatgccgag ggccgagcag atctggcccg gcgtgcagta accgcactgg aaggcatcgt
     6781 gcttcacgaa ggccgcctgc atcggatgca gccgtccggg cgtgccgagg ccctcgatcg
     6841 tcgtgagctc ggcgccctcg tgcatgacgg cgagcgtcag gcaggcgttg atgcgccggc
     6901 cgtcgagcag caccgtgcag gccccgcact ggccgtgatc gcagcccttc ttggtgccgg
     6961 tcagctgcag atgctcgcgc agcgcatcga gcagcgtcgt gcgcgtatcg agttcgaggc
     7021 tgtgcacctc gccgttgatg cgcagcctca cgcgctgttg caccggaact gccgcggttt
     7081 gcggcgcatc gcggcttgcg gcgcccgaca gcgacggcag cgccgccgca ctggccgaca
     7141 cggcgctcgc gatcagcagc cggcggcggg tgatcttcag atgcaggcag tcgtccacgg
     7201 gttgttttcc tcgacgcggg cgggaagacg gaccggtcgt ggtaccgcag gcagcgaggc
     7261 agcctgacag ccggccggca gcggcggact tcggctccgg acgggccgga acggaaacgg
     7321 cagaagccgg cggcccggcg caggccggcc gtcagcccgg gtttcgagcc acgcccgcgc
     7381 gctgcgtgcc ggccgcgcac gacctgctgc tatgcacgaa tcgtccatcg gcacggcgcc
     7441 ggccgtgaca ggtccggcca cgccgggcgc cggcggggct gatgccgggg gtctgcagtc
     7501 gaaaacaggt gcctgcgcaa cccgtaatca gcccgtaatc gccgctctgg ccggttttcg
     7561 cactgtttgc cctgataagt tcaataatca atcaaatcct gcagatatga tgatttttat
     7621 aactgtatta accgataaat tatcaattct gctgctctgg acggagctca tgtcggccag
     7681 ccctgcaagt cgtcacatgc gcagggagca ttccccgctg cctttcttcg cgcgtgcgca
     7741 ggactgcgct gccggatgaa cgcacgggcg gcgcccaggg ctcagagatc cgcgaactgc
     7801 cagtcggcct gcgtgacgac gcgatcgatg
//
LOCUS       DAOQJH010000019         7830 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_5060_length_7830_cov_3.649260, whole genome shotgun sequence.
ACCESSION   DAOQJH010000019 DAOQJH010000000
VERSION     DAOQJH010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 7830)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7830)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7830
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5060_length_7830_cov_3.649260"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..335
                     /locus_tag="PLQ18_00910"
     CDS             <1..335
                     /locus_tag="PLQ18_00910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE00235.1"
                     /translation="RCLAACRLHPQAGRLPVRGKSWPGAGSGLPVGGNRPGISGSQPG
                     VIANMVSVEAKLVLFEVKPVLSAVELVMSEVKPVLSGGELVMSGVKPGFVLSGTGYVR
                     SETGFRFK"
     gene            complement(819..4262)
                     /gene="mfd"
                     /locus_tag="PLQ18_00915"
     CDS             complement(819..4262)
                     /gene="mfd"
                     /locus_tag="PLQ18_00915"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018953916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HNE00236.1"
                     /translation="MPPKSAASPATALPALPLPADALKPGRWSGLSEAAQALAIAATA
                     ARTQALLLVLAPDTQTALRLDAAIGWFAGTLDVPRLVFPDWETLPYDQFSPHQDLISQ
                     RLATLYRLPMQKRGVLIVPAPTLMQRLGPKSFLDAHALLLASGDRLDVDAFRTRLEAA
                     GYLCVAQVMEHGEFAVRGSIIDVFPMGAEAPLRIDLFDDEIESLRSFEPETQRSIAKL
                     DAIELLPAREFAMTRESITRFRTNFRATFEGDPQQSPIYREVSQGFAPPGIEYWLPLF
                     FEQTSTLFDFLPSNALVLRLPGWEAAMDGFIAQVQERYESRRHDRTRPLLAPAQLFLD
                     AGEVNRRVSRLLRVDLDSTGAPALPELYFAPRAERPAAALLDWLDAFPGRVLFAAESA
                     GRREVLAETLRAQNLLPVAVDGWPGFVGSEARLALTVAPLDQGFVLEDGAAAPLAVIT
                     ESLLHREWVRSTRRSSRAAREAEAIIRNLADLAIGAAVVHEDHGVGRYQGLVHMEAGG
                     IPAEYLVLEYANAEKLYVPVAMLDRVSRYTGGENPPLHRLGGEQWEKARRKAAEKAHD
                     AAAELLDVYARRAARPGHKFHGDEREMLAFAAAFPYEETPDQQLAIDAVLADLRTGKP
                     MDRVVCGDVGVGKTEVAMRAAFLAVQDGRQVAVLVPTTLLANQHYRNFLDRYAGWPVT
                     IESLSRFRSKKDTDAVLAGLASGGIDIVIGTHKLIQPEVRFKDLGLVIIDEEHRFGVR
                     QKEAFKALRSEVDILTLTATPIPRTLNFALAGLRELSIIATPPASRLAVKTFVNEYNA
                     ALIEEACLRELKRGGQVYFLHNEVDSIHRRAQELAQLVPGARVAVAHGQMGAGELEKV
                     MLDFYHQRCNLLVCTTIVESGIDVPSANTIIIERADKLGLAQLHQLRGRVGRSHHRAY
                     CYLIVPNRKSMTADAVKRIEAIESLEELGAGFMLANHDLEIRGAGELLGEEQSGQIHE
                     VGYTMYMDMLERAVKAIRSGRTPALDRPLDHGPEVSLGVPALIPEDYLPDVHARLMLY
                     KRIASCTDAAALQELQVEMIDRFGLLPAQVQNLFRITELKLAAGPVGIRKIEAGPEGG
                     RIVFGPEPNIDVSRLLVLIHKQPKTYRLDGQDKLRIQKPLADAEARAAEVRMLLGQIA
                     A"
     gene            4509..5168
                     /locus_tag="PLQ18_00920"
     CDS             4509..5168
                     /locus_tag="PLQ18_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015281474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE00237.1"
                     /translation="MIRVMLVDDHQLIRIALGQILGGHADIEVVAEAADGEEALVRAR
                     AAKPDVILIDVDMPGMGGVEATRRLSALAHKPRVVAISVHDQMPYPQRMMEAGAVGYL
                     PKGGSADEVLAAVRAVARGQPYIAPVIAGKMFLTSMNRSGSAGNGAQVLDTLSAREMQ
                     VMLALVNGQNPQQIGEALKISAKTVCTHRYHIYEKLGVDSDVALTHLAYRYGLIEVSQ
                     S"
     gene            complement(5185..5523)
                     /locus_tag="PLQ18_00925"
     CDS             complement(5185..5523)
                     /locus_tag="PLQ18_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020481603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6156 family protein"
                     /protein_id="HNE00238.1"
                     /translation="MTTETESAAGRGHCRLFVTYSGVRLPLKLSGPIEDTAHRNTYFR
                     GWFDAGERVTEIEKLVYGEVEMRHRYAYHANGTLAQAEITDADGEIRVLHFDAQGAPL
                     AHDDDDDDDE"
     gene            5687..6223
                     /locus_tag="PLQ18_00930"
     CDS             5687..6223
                     /locus_tag="PLQ18_00930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE00239.1"
                     /translation="MQTVTAQEDLRLRAALEVQRYRLGRSLRGCAAAALTGLLLALGG
                     LHEDRLPTPLALGFLVTLGGFASLGALLLQRRFARLARVVPRLRATPCLALLAVGGWF
                     QEPEYQADVRIPGLWHWHVDLETPLPDPHLEAGGDQPIDAIAWLEPRRHRLWALELRT
                     VVFWVRRCVRVEPATRRA"
     gene            complement(6243..>7830)
                     /gene="hrpB"
                     /locus_tag="PLQ18_00935"
     CDS             complement(6243..>7830)
                     /gene="hrpB"
                     /locus_tag="PLQ18_00935"
                     /inference="COORDINATES: protein motif:HMM:TIGR01970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HNE00240.1"
                     /translation="VSRAAAAQRAGRAGRLGPGLCLRLWTAATDSTLAPFSAPEILEA
                     DLAPLALELAAWGAAAGALVWLDPPNPGALAQAQELLRGLDALDADARITAQGRALLA
                     LGLHPRLGHLLLRGRALGLGALACDLAALLDERDPLRGEAARSADLTLRLEALAAVRA
                     GAREAARGFGADANACARLLEAARVLRERLDVPAADPRASIRSQDLALLVAAAYPDRI
                     ARRRDGSPDRFLLASGRGARLREGDALTGSDWLAVAALDAGEREGRIFLAVALDLPTL
                     ETALAGHIAEVAEVRWDEREQAVVARRECRLGALLLDARALARPEPAALRRAMCAGIE
                     LLGLDALPWTDALRDWQKRVQSLRHWQPEAGWPEVSDAALAATLGDWLQPYLDGCTRR
                     EHLARLDLAAILHARLDWPQTQRLAALAPTHLDVPSGSRLRLEYAADGAAPVLAVKLQ
                     EMFGLADTPRIADGRIAVTLHLLSPARRPIQVTQDLRGFWERTYAEVRKELKGRYPKH
                     PWPDDPWTAVPTARAKPRGR"
BASE COUNT         1077 a         2653 c         2946 g         1154 t
ORIGIN      
        1 gccggtgtct ggctgcctgc cggctgcatc cgcaggccgg caggttgccg gtgcgcggga
       61 agagctggcc gggggcgggc agcggcttgc cggtgggggg gaaccggcca ggtatttccg
      121 gaagtcagcc tggggtgatt gccaacatgg tttccgttga agcgaaactg gttctgttcg
      181 aagtgaaacc agttctgtcc gcagtggaac tggttatgtc tgaagtgaaa ccagttctgt
      241 ccggagggga actggttatg tccggagtga aaccgggttt cgttctgagt ggaactggtt
      301 atgtccggag tgaaaccggg tttcgtttca agtgaaaccg gttatgtccg gagcggaact
      361 ggttctgtcc ggaacgaaac cggttatgtc cggaggagaa ctggttgtgt ccgcaacgaa
      421 tccgggtttc ggttcgagtg caaccggttc ggtccgcagg ggaactgctt cggtacgcag
      481 cgcaacccgg tccgtgccgg ggggagcgga ttgcgggcgc ggggcagccg gctgcgctgc
      541 ggtggcagcg ggcgggaggg cgatggcagc cggggctggc caggatcgag ccgggtgcgg
      601 tcgatgccgg cgcggtacgg gcggtgggcg aaccggttac ggatgtcgcg ggctggcttg
      661 cggcagcggc gatggccggt ggagcggtcg ggcagcgggg cgcgggtgag ggggcgtcgg
      721 gacaggacgc aagaaatccg gccaggatgc agaagatcag gatgcgcccg ccccacggct
      781 gcgggcttga tttccggcac cggctccgcg ccgccgcttc aggccgcgat ctgcccgagc
      841 agcatgcgca cctcggcggc ccgcgcctcg gcgtcggcca gcggtttctg gatgcgcagc
      901 ttgtcctgac catcgagccg gtaggttttc ggctgcttgt ggatcagcac cagcaggcgg
      961 ctcacgtcga tgttcggctc cggcccgaac acgatgcgcc cgccctccgg gccggcctcg
     1021 atcttgcgga tgccgaccgg gccggcggcg agcttcagtt cggtgatgcg gaacaggttc
     1081 tgcacctgcg ccggcagcag gccgaagcgg tcgatcatct cgacctgcag ctcctgcagc
     1141 gccgcggcat cggtgcagct ggcgatgcgc ttgtacagca tcagccgcgc gtgcacgtcg
     1201 ggcaggtagt cctcggggat cagcgccggc acgccgaggc tgacctcggg gccgtgatcg
     1261 agcgggcggt cgagcgccgg cgtgcgcccg gaccggatcg ccttcaccgc gcgttcgagc
     1321 atgtccatgt acatcgtgta gccgacctcg tggatctggc cgctctgctc ctcgccgagc
     1381 agttcgccgg ccccgcggat ctccaggtcg tggttggcga gcatgaagcc ggcgccgagt
     1441 tcttcgagcg attcgatggc ctcgatgcgc ttgacggcat cggcggtcat gctcttgcgg
     1501 ttcggcacga tcaggtagca gtaggcgcgg tggtgcgagc ggccgacgcg gccgcgcaac
     1561 tggtgcagct gcgccagccc gagcttgtcg gcgcgctcga tgatgatggt gttggcgctc
     1621 ggcacgtcga tgccggattc gacgatggtc gtgcacacca gcaggttgca gcgctggtga
     1681 tagaaatcga gcatgacctt ttcgagctcg ccggcgccca tctggccgtg cgcgaccgcg
     1741 acgcgcgcgc cgggcacgag ctgcgcgagt tcctgcgcgc ggcggtggat gctgtcgacc
     1801 tcgttgtgca ggaagtacac ctgtccgccg cgcttcagtt cgcgcaggca ggcttcctcg
     1861 atcagggctg cgttgtactc gttgacgaag gtcttgaccg caagccggct cgccggcggc
     1921 gtggcgatga tcgacagctc gcgcagcccg gcgagcgcga agttcagcgt gcgcgggatc
     1981 ggcgtggcgg tcagcgtcag gatgtcgacc tcggagcgca gggccttgaa ggcttccttc
     2041 tggcgcacgc cgaagcggtg ttcctcgtcg atgatgacga gaccgaggtc cttgaaccgc
     2101 acttcgggct ggatcagctt gtgcgtgccg atgacgatgt cgatgccgcc gctggcgagg
     2161 ccggcgagca cggcgtcggt gtccttcttc gagcggaagc gcgacaggct ctcgatggtc
     2221 accggccagc cggcgtagcg gtcgaggaag ttgcggtagt gctggttggc gagcagcgtc
     2281 gtcggcacca gcaccgccac ctgccggcca tcctgcacgg cgaggaaggc cgcgcgcatc
     2341 gcgacctcgg tcttgccgac gccgacatcg ccgcagacga cgcggtccat cggcttgccg
     2401 gtgcgcaggt cggcgagcac ggcgtcgatc gcgagctgct ggtccggggt ttcctcgtac
     2461 ggaaaggccg ctgcgaaggc gagcatctcg cgctcgtcgc cgtggaattt gtgccccggg
     2521 cgggcggcgc ggcgggcgta gacatcgagc agctcggcgg cggcatcgtg cgccttctcg
     2581 gcggccttgc gccgcgcctt ctcccactgc tcgccgccga ggcggtgcag cggcgggttc
     2641 tcgccgccgg tgtagcggct gacgcgatcg agcatcgcca ccggcacgta gagtttttcg
     2701 gcgttcgcgt attcgagcac gaggtattcg gccgggatgc cgccggcctc catgtgcacg
     2761 aggccctgat agcggccgac gccgtgatcc tcgtgcacga ccgccgcgcc gatggcgagg
     2821 tcggcgaggt tgcggatgat ggcctcggcc tcgcgcgcgg cgcgactgct gcgccgtgtg
     2881 ctgcgcaccc attcgcggtg cagcaggctc tcggtgatga cggcgagcgg tgccgcggcg
     2941 ccgtcctcga gcacgaagcc ctgatcgagc ggcgcgaccg tcagcgcgag ccgcgcctcg
     3001 ctgccgacga aacccggcca gccgtcgacc gcgaccggca gcagattctg cgcacgcagc
     3061 gtctcggcca gcacctcgcg ccggccggcc gattcggcgg cgaacagcac gcgccccgga
     3121 aaggcatcga gccagtcgag cagtgccgcg gccgggcgct cggcgcgcgg tgcgaagtac
     3181 agctccggca gcgccggggc gccggtgctg tcgagatcga cgcgcagcag ccggctgacg
     3241 cggcggttga cctcgccggc gtcgagaaag agctgcgccg gggcgagcag cgggcgcgtg
     3301 cggtcgtggc ggcggctctc gtagcgctcc tgcacctgcg cgatgaagcc gtccatcgcc
     3361 gcttcccagc cgggcaggcg cagcaccagc gcgttcgagg gcaggaagtc gaacagcgtc
     3421 gaggtctgct cgaagaacag cggcagccag tattcgatgc cgggcggcgc gaagccctgg
     3481 ctgacctcgc ggtagatcgg gctctgctgc gggtcgccct cgaaggtggc gcggaagttc
     3541 gtgcgaaagc gcgtgatcga ttccctggtc atcgcgaact cgcgcgccgg cagcagctcg
     3601 atcgcatcga gcttcgcgat cgagcgctgc gtctcgggct cgaagctcct caggctctcg
     3661 atttcgtcat cgaacaggtc gatgcgcagc ggcgcctccg cgcccatcgg gaacacgtcg
     3721 atgatcgagc cgcgcaccgc gaactcgccg tgctccatga cctgcgcgac gcagagataa
     3781 ccggccgctt ccaggcgcgt gcggaaggca tcgacatcga gccggtcgcc gctggcgagc
     3841 agcagcgcgt gcgcatcgag gaagctcttc gggccgaggc gctgcatcag cgtcggcgcc
     3901 ggcacgatca gcacgccgcg cttctgcatg ggcaggcggt acagcgtcgc cagccgctgc
     3961 gagatcagat cctgatgcgg cgagaactgg tcgtacggca gcgtctccca gtccgggaac
     4021 accagccgcg gcacatcgag cgtgccggcg aaccagccga tcgccgcatc gaggcgcagc
     4081 gccgtctgcg tgtccggcgc cagcaccagc agcagcgcct gcgtgcgcgc cgccgttgcg
     4141 gcgatcgcca gcgcctgcgc ggcttcggac aggccgctcc agcggccggg cttcagcgca
     4201 tcggcgggca gcggcagggc gggcagggcg gtggcagggc tggcggcgga cttcggcggc
     4261 atcggcaaga cacgtgcgga ctgaggcggc aggcgcgggg gcgccatgat agccgcgtgc
     4321 cagaggctgg cggccgatcg tgaggactgg ttacgaccga tgtcgcgggc gcggcggaca
     4381 gtgcaggctg aaaggcgttg tcctgcccgg gcagcctgag aattttctga taactttttg
     4441 cgaaccggcg cagccggccg gctgcgcagc ctttccagat tcctcccccc gagcaggttc
     4501 ccgccgcgat gatccgcgtc atgctggtcg acgatcacca actgatccgc atcgccctcg
     4561 gccagatcct cggcggccac gccgacatcg aggtcgtcgc cgaggcagcc gacggcgagg
     4621 aggcgctggt gcgtgcgcgc gccgccaagc ccgacgtgat cctgatcgat gtcgacatgc
     4681 ccggcatggg cggcgtcgag gccacgcgcc ggctgtcggc gctcgcgcac aagccgcgcg
     4741 tggtcgcgat ctcggtgcac gaccagatgc cgtatccgca gcggatgatg gaggccggcg
     4801 ccgtcggcta cctgcccaag ggcggttcgg ccgacgaggt gctggccgcg gtgcgcgccg
     4861 tggcccgcgg ccagccgtac atcgcgccgg tgatcgccgg caagatgttc ctgacctcga
     4921 tgaaccgcag cggcagcgcc ggcaacggcg cgcaggtgct cgacacgctg tcggcgcgcg
     4981 agatgcaggt gatgctggcg ctggtcaacg gccagaaccc gcagcagatc ggcgaggcgc
     5041 tgaagatcag cgccaagacc gtctgcacgc accgctatca catctacgag aagctcggcg
     5101 tcgacagcga cgtggcgctg acgcacctcg cctaccgcta cgggctgatc gaagtctcgc
     5161 agtcctgagc cggacggcgg cgggtcattc gtcgtcgtcg tcgtcgtcgt cgtgcgcgag
     5221 cggcgcaccc tgcgcgtcga aatgcagcac gcggatttcg ccgtcggcat cggtgatctc
     5281 ggcctgcgcc agcgtgccgt tcgcgtggta ggcgtagcgg tggcgcatct cgacctcgcc
     5341 gtagacgagc ttctcgatct cggtcacgcg ctcgccggca tcgaaccagc cgcggaagta
     5401 ggtgttgcgg tgcgcggtgt cctcgatcgg gccgctgagc ttcagcggca gccggacacc
     5461 ggaataggtc acgaacaggc ggcagtggcc gcgtccggca gcggattcag tttctgtggt
     5521 catcggagcc attcccggtt gcagggggtc atgcacaggg cgccggcccg gcggcgcgca
     5581 gtctgacggc cgccgccggc agcgtccaca ctgagggggc cgggccgggc gccgtgcgca
     5641 gcgtccgctc ccgtccgccc gtcgcgtgca gccgaggtgc cccgagatgc agaccgtcac
     5701 cgcccaggaa gacctgcgcc tgcgcgccgc gctcgaagtc cagcgttacc ggctcgggcg
     5761 ctcgctgcgc ggctgtgccg ccgccgcgct caccggcctg ctgctcgcgc tcggcggcct
     5821 gcacgaggac cggctgccga cgccgctcgc gctcggcttc ctggtcacgc tcggcggctt
     5881 cgcgagcctc ggcgcgctgc tgctgcagcg gcgcttcgcg cggctcgcgc gcgtcgtgcc
     5941 gcggctgcgg gccacgccct gtctggcgct gctcgcggtc ggcggctggt tccaggagcc
     6001 cgagtaccag gccgatgtgc gcatccccgg cctctggcac tggcacgtcg atctcgaaac
     6061 cccgctgccc gacccgcacc tcgaagccgg cggcgatcag ccgatcgatg cgatcgcgtg
     6121 gctggagccg cgccggcacc ggctgtgggc gctggagctg cgcaccgtgg tgttctgggt
     6181 gcggcgctgc gtgcgcgtcg agccggccac gcgccgcgcc tgaagcccgc ccgggccggg
     6241 cttcagcggc cccgcggttt cgcccgcgcc gtcggcaccg ccgtccacgg atcgtccggc
     6301 cacgggtgct tcgggtagcg ccccttcagt tccttgcgca cctcggcgta ggtgcgttcc
     6361 cagaagccgc gcaggtcctg cgtgacctgg atcgggcggc gggccggcga cagcaggtgc
     6421 agggtcacgg cgatgcggcc gtcggcgatg cgcggcgtgt cggcgaggcc gaacatctcc
     6481 tgcagcttca cggccagcac cggcgcggca ccgtcggcgg cgtattcgag gcgcaggcgc
     6541 gagccgctcg gcacgtcgag atgcgtcggg gccagcgcgg cgaggcgctg cgtctgcggc
     6601 cagtcgaggc gggcgtgcag gatcgcggcg agatcgaggc gggcgaggtg ctcgcggcgc
     6661 gtgcagccgt cgagatacgg ctgcagccag tcgccgagcg tcgccgcgag cgcggcatcg
     6721 gaaacctccg gccagccggc ctccggctgc cagtggcgca gcgactgcac gcgcttctgc
     6781 cagtcgcgca gcgcatcggt ccacggcagc gcgtcgaggc cgagcagctc gatcccggcg
     6841 cacatggccc ggcgcagcgc cgccggctcg ggacgcgcga gcgcgcgggc gtcgagcagg
     6901 agcgcgccga gccggcactc gcgccgggcg acgacggcct gctcgcgctc gtcccagcgc
     6961 acctcggcga cctcggcgat gtggccggcg agggcggtct ccagcgtcgg caggtcgagc
     7021 gccacggcga ggaagatgcg gccttcacgc tcgccggcgt cgagcgcggc gactgcgagc
     7081 cagtcgctgc cggtgagtgc atcgccctcg cgcagccgcg caccgcggcc gctggcaagc
     7141 aggaaacgat ccggactgcc gtcgcggcgc cgggcgatgc gatccggata ggcggcggcg
     7201 accagcagcg ccaggtcctg actgcggatc gacgcgcgcg ggtcggcggc cggcacgtcg
     7261 agccgctcgc gcagcacgcg cgcggcttcg agcaggcgcg cgcaggcatt ggcatcggca
     7321 ccgaagccgc gtgcggcctc gcgtgcaccg gcgcgcaccg cagccagggc ttcgaggcga
     7381 agcgtcagat cggcgctgcg cgcggcctcg ccgcgcagcg gatcgcgctc atcgagcagc
     7441 gcggcgagat cacaggcgag cgcgccgagt ccgagcgcgc gcccgcgcag cagcaggtgg
     7501 ccgaggcgcg ggtgcaggcc gagcgcgagc agcgcgcggc cctgcgcggt gatgcgggcg
     7561 tccgcatcga gcgcatcgag cccgcgcagc agttcctgcg cctgcgccag cgcgccggga
     7621 ttcggcggat cgagccagac cagcgccccg gccgccgcgc cccaggcggc gagttcgagc
     7681 gcgagcgggg cgaggtcggc ttcgaggatc tcgggcgccg agaacggcgc cagcgtcgaa
     7741 tcggttgccg ccgtccagag ccgcaggcac aggccggggc cgaggcgccc ggcgcgcccg
     7801 gcgcgctgcg cggcggcggc gcggctgacg
//
LOCUS       DAOQJH010000020         7828 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_5062_length_7828_cov_4.245980, whole genome shotgun sequence.
ACCESSION   DAOQJH010000020 DAOQJH010000000
VERSION     DAOQJH010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 7828)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7828)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7828
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5062_length_7828_cov_4.245980"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..1030)
                     /locus_tag="PLQ18_00940"
     CDS             complement(2..1030)
                     /locus_tag="PLQ18_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004353805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HNE00241.1"
                     /translation="MSRFAPRFLRPLLAVACAVVLTVGGSARADEPRGLLNVSYDVAR
                     EFYKDVNAQFVPYWQRSHGETVTVNQSHGGSSKQARAVIDGLAADVVTMNQQTDIDAI
                     AKTGLIRSDWRQALPFGSAPYGSTIVFLVRKGNPKGIQDWPDLGRDGVQIVIPNPKTA
                     GNGRYSYLGAWAWAKEKYGSDEAAREFVGKVFRNVPVLDAGGRAATTTFMQRGIGDVL
                     LTFESEVLQITREFSPDAFDVVIPSLSIDADAPVAVVDKVVDRKGSRALAEGYLRFLY
                     TPEGQRLAAKHYLRPRDPAVFAEFREQFRNVRLVTVDAAFGGWPQAQATHFADGGEFD
                     RLYQPGSR"
     gene            complement(1149..1739)
                     /locus_tag="PLQ18_00945"
     CDS             complement(1149..1739)
                     /locus_tag="PLQ18_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017717963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HNE00242.1"
                     /translation="MSLPQACTRFTFEDYLVWEPTQELRHEYLNGEVFAMTGARRNHV
                     TVTVNLTSALRNLVRGTPCRVYASDMKLRIASADAACYPDVSVSCDERDRRTDLFIAH
                     PLLIIEVLSPSTAAWDRSRKFDLYRQIDTLQEYVLIEPELRSVDVFRRRPAGDWLFQP
                     FRDDEAVELAALGCTLPMALIYEDVDFPPDAAADPD"
     gene            1897..3264
                     /gene="miaB"
                     /locus_tag="PLQ18_00950"
     CDS             1897..3264
                     /gene="miaB"
                     /locus_tag="PLQ18_00950"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008931212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HNE00243.1"
                     /translation="MPRKLFIKTHGCQMNEYDSAKMGDVLRESHGLELTDDPAQADVL
                     LLNTCSIREKAQEKVYSELGRWRELKLERPGLVIGVGGCVASQEGAALRERAPFVDVV
                     FGPQTLHRLPALLAAADEHRGAVVDVSFPEIEKFDSLPEPRAEGPTAFVSVMEGCSKY
                     CTFCVVPYTRGEEVSRPFDDVLVEVATLAAQGVREVTLLGQNVNAYRGVMADGSIADF
                     AALIEVVAAIDGIDRIRYTTSHPIEFSDRLIEVYASVPELVDHLHLPVQSGSDRILAA
                     MKRRHTVLEYKSKIRRLRAIRPDLSLSTDIIVGFPGETEADFQATLKLIDEIGYDHSF
                     SFLYSPRPGTPAAELPDDVPLEQKKARLQLVIDRFNANAARIAAGMVGTVQRILVERH
                     ARKDARQLAGRTPNNRVVNFDGPERLIGHFVDVRITEALPNSLRGEFVACEEIGMPAA
                     TVANA"
     gene            3285..4280
                     /locus_tag="PLQ18_00955"
     CDS             3285..4280
                     /locus_tag="PLQ18_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018953703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HNE00244.1"
                     /translation="MPAADGAVTQVLDLEPADNARLAQLCGHLDEHLRQIERRLGITI
                     ANRGGHFSLNGPEAATRIGAAVIEALYRAAGEEELNPARVHLFLQEAGADDIELPDPV
                     EAEPEEVAIRTRRGIVRGRGINQQRYLYAIQRHDINFGVGPAGTGKTYLAVACAVEAL
                     ERDEVRRLVLVRPAVEAGERLGFLPGDLVQKVDPYLRPLYDALYEMLGFERVAKLMER
                     SVIEIAPLAFMRGRTLNDAFIILDEAQNTTVEQMKMFLTRIGFGSTAVITGDTSQVDL
                     PKGVRSGLAHALEVLDGVDGISMTRFLPRDVVRHPLVQRIVIAYERHEQKQGGSA"
     gene            4277..4756
                     /gene="ybeY"
                     /locus_tag="PLQ18_00960"
     CDS             4277..4756
                     /gene="ybeY"
                     /locus_tag="PLQ18_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012074438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HNE00245.1"
                     /translation="MSRLALDLQIETGAGALPPAADFRRWAEAALAGADFHDPCEMSV
                     RIVGIAESAALNAGYRHKDGPTNVLSFPFETPPGIVLDAELLGDLVICAPVVVREAGE
                     QHKPEAAHWAHLTVHGTLHLLGYDHLDDAEAEAMEALEIRILAGLGYPNPYADDERP"
     gene            4753..5631
                     /locus_tag="PLQ18_00965"
     CDS             4753..5631
                     /locus_tag="PLQ18_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015279654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HNE00246.1"
                     /translation="MKEDRSGDSTGTTGRSWLERLGQALAGGPEDRDDLKDILKEAQA
                     RGIIDAETLSMMEGALTVAERQVRDVMIPRGQMIVMQADWPVERLLAEIIESGHSRLP
                     VIGEDRDEVLGILLVKDLLRWFASGAGEFDLRSFLRAPTFIPESKRLDVLLREFRANR
                     LHMAIVVDEYGGVAGLVTIEDLIEQIVGEIDDEHDEAGDDGDIRQIGEHEYIVRALTP
                     LADFNVRFDAHFDEDEADTVGGLVLLDLERVPKRGEVVEIERFRFRVLRADNRRVHLF
                     GLSVLDPVVPVDAANA"
     gene            5722..6372
                     /locus_tag="PLQ18_00970"
     CDS             5722..6372
                     /locus_tag="PLQ18_00970"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011439348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HNE00247.1"
                     /translation="MDEWTQRRARMVEEQIEARGIHDPDVLAAMRRVPREDFVPEAMR
                     AWACDDSPLSIGDGQTISQPYIVALMIAAAAIRPGGRVLEVGTGSGYAAAVMAELAAH
                     VYTIERIEALAAQAAGRFARLGCTNIELRTGDGSGGWPEAAPFDAILVAASGPCVPEV
                     LRAQLAPGGRLVMPVDDGWLQRLVCVTREAAGSYREDFLCDVRFVPLIGAHGWEGA"
     gene            complement(6479..6913)
                     /locus_tag="PLQ18_00975"
     CDS             complement(6479..6913)
                     /locus_tag="PLQ18_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012352822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNE00248.1"
                     /translation="MLPPGFYAGTFPDSAVGTVHRAPGDYPSGQEGDVLMVESTVIGI
                     PCPGLNGGPMFRHSEAFSFQVATDDQAETDRLWNAIVSNGGQESACGWYRDPWGLSWQ
                     ITPRALTAAIADPDRAAARRAFEAMMQMGKIDIAAIEAARRG"
     gene            7004..>7828
                     /locus_tag="PLQ18_00980"
     CDS             7004..>7828
                     /locus_tag="PLQ18_00980"
                     /inference="COORDINATES: protein motif:HMM:NF039484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HNE00249.1"
                     /translation="MTAPLADPAASPSAVSSGSTDSGAPAAGRLAAGHPLALLLAPIA
                     GLLLPLAFAPYRLWPLGLLLPAVLLWLIDGERPRRALLIGLLFGLGSYGHGIWWIYIS
                     LHRYGGAPPAFAGLATFLVVLLMAAYVALFAGLLNRLAPAPGVRRWLLAAPGLWALLE
                     WVRSWAFTGFPWLALGYAQVESPLAGFAPILGTFGLGALVMLVAGSLRLALARLRSGA
                     RAALAPLAVALLVPALGFGLGRIDWSAPAGRVLAVGLAQGNIAQEMKWRPGKLEETI"
BASE COUNT         1162 a         2699 c         2730 g         1237 t
ORIGIN      
        1 gtcagcggct gccgggctgg tagagacggt cgaactcgcc gccatcggcg aagtgggtcg
       61 cctgcgcctg cggccagccg ccgaaggcgg catcgacggt cacgaggcgc acgttgcgga
      121 actgctccct gaattcggcg aacaccgccg gatcgcgcgg gcgcagatag tgcttcgcgg
      181 cgaggcgctg gccctcgggc gtgtacagga agcgcaggta gccctcggcg agggcgcggc
      241 tgcccttgcg atcgacgacc ttgtcgacga cggcgaccgg cgcatcggca tcgatgctga
      301 gcgacgggat cacgacatcg aaggcgtcgg ggctgaattc cctggtgatc tgcagcactt
      361 cgctctcgaa ggtcagcagc acgtcaccga tgccgcgctg catgaaggtc gtcgtcgcgg
      421 cacggccgcc ggcgtcgagc accggcacgt tgcggaacac cttgccgacg aactcgcgcg
      481 cggcctcgtc gctgccgtat ttttccttcg cccaggccca ggcgccgagg tagctgtagc
      541 ggccgttgcc ggccgtcttc gggttcggga tgacgatctg cacgccgtcg cgcccgagat
      601 cgggccagtc ctgaatgcct ttcgggttgc ccttgcgcac caggaagacg atggtcgagc
      661 cgtacggcgc cgagccgaac ggcagcgcct gacgccagtc gctgcggatc aggccggtct
      721 tcgcgatcgc gtcgatgtcg gtctgctggt tcatcgtcac gacatcggcg gcgagcccgt
      781 cgatgacggc gcgcgcctgc ttgctggagc cgccgtgcga ctggttgacg gtgacggtct
      841 cgccgtggct gcgctgccag tacggcacga actgcgcgtt cacgtccttg tagaactcgc
      901 gcgccacgtc gtacgagacg ttcagcaggc ccctgggctc atcggcgcgg gcactgccgc
      961 cgacggtgag cacgactgcg caggcgacag cgagcagcgg gcggagaaag cggggggcga
     1021 agcgggacat gcggcaatac ctcggcagga gcgcatctca tggcggccga gcatatccgc
     1081 ggattttata ttgcttaaaa taataatgaa ttagttttat attctttttt tgtataaatg
     1141 cgcgtgcatc agtccggatc ggcagcagcg tcgggcggaa aatccacatc ctcgtagatc
     1201 agcgccatcg gcagcgtgca gccgagcgcg gccagttcga ccgcctcgtc gtcgcgaaac
     1261 ggctggaaca gccagtcgcc ggcgggcctg cgccggaaca cgtcgacgct gcgcagctcc
     1321 ggctcgatca gcacgtattc ctgcagcgtg tcgatctgcc ggtacagatc gaacttgcga
     1381 ctgcggtccc aggcggccgt cgagggcgac agcacctcga tgatcagcag cggatgggcg
     1441 atgaacagat cggtgcgccg gtcgcgttcg tcacagctca cgctcacgtc cgggtagcag
     1501 gccgcatcgg cgctggcaat gcgcagtttc atgtcgctgg catagacgcg gcagggcgtc
     1561 ccgcgcacga gattgcgcaa ggccgacgtc aggttgacgg tcaccgtgac gtggttgcga
     1621 cgggcgccgg tcatcgcgaa gacttcgccg ttcaggtact cgtggcgcag ctcctgcgtc
     1681 ggctcccaga ccagatagtc ctcgaacgtg aaacgggtgc aggcttgcgg caggctcatg
     1741 tccgtgactc cgggcaggcg acgggtccga gtatggcccg gctcgccggg ccgtcgcgag
     1801 tgtttgtcgg cgccggcgcc ggtgctagac tcccgccccc gcatcccctt gagtgcgttg
     1861 aaaactttac tgacgctgct ccgtcgcagg aacccgatgc cgcgcaaact ctttatcaag
     1921 actcacggtt gccagatgaa cgagtacgac tccgcgaaga tgggagatgt actgcgcgag
     1981 tcacacggtc tggaactcac cgacgatccc gcccaggccg atgtcctgct gctgaacacc
     2041 tgttcgatcc gcgagaaggc gcaggagaag gtctactccg agcttggccg ctggcgcgag
     2101 ctgaagctcg aacgcccggg cctcgtcatc ggcgtcggcg gctgcgtcgc cagccaggaa
     2161 ggcgcggcgc tgcgcgagcg cgcgcccttc gtcgacgtgg tgttcggccc gcagacgctg
     2221 caccggctgc cggcgctgct cgcggcggcc gacgagcacc gtggcgccgt cgtcgacgtg
     2281 tccttccccg agatcgagaa gttcgacagc ctgcccgagc cgcgcgccga gggaccgacc
     2341 gcgttcgtgt cggtcatgga aggctgctcg aagtactgca ccttctgcgt cgtgccgtac
     2401 acgcgcggcg aggaggtctc gcggccgttc gatgacgtgc tggtcgaggt cgcgacgctg
     2461 gcggcgcagg gcgtgcgcga agtcacgctg ctcggccaga acgtcaacgc ctaccgcggc
     2521 gtcatggccg atggctcgat cgccgatttc gcggcgctga tcgaggtggt cgcggcgatc
     2581 gacggcatcg accgcatccg ctacacgacc tcgcatccga tcgagttcag cgaccggctg
     2641 atcgaggtct atgccagcgt gccggaactg gtcgatcacc tgcacctgcc ggtgcagagc
     2701 ggctcggacc gcatcctcgc ggcgatgaag cgccggcaca cggtgctcga atacaagtcg
     2761 aagatccgca gactgcgcgc gatccggccg gatctgtcgc tgtcgaccga catcatcgtc
     2821 ggcttccccg gcgagaccga ggccgatttc caggcgacgc tgaagctgat cgacgagatc
     2881 ggctacgacc acagcttcag cttcctgtac agcccgcgcc cgggcacgcc ggccgccgaa
     2941 ctgcccgacg atgtgccgct ggagcagaag aaggcgcggc tgcagctcgt catcgaccgc
     3001 ttcaacgcca atgccgcgcg catcgccgcc ggcatggtcg gcacggtgca gcgcatcctg
     3061 gtcgagcgcc acgcgcgcaa ggacgcccgc cagctcgccg ggcgcacgcc gaacaaccgc
     3121 gtcgtcaact tcgacgggcc cgagcgcctg atcgggcatt tcgtcgacgt gcgcatcacc
     3181 gaggccctgc cgaactcgct gcgcggcgag ttcgtcgcct gcgaggagat cggcatgccg
     3241 gctgcgacgg tggcgaacgc gtgaataccg tcctgcccgc accgctgccc gccgccgacg
     3301 gtgccgtcac gcaagtcctc gacctcgaac ccgccgacaa cgcccgtctc gcccagctct
     3361 gcggccacct cgacgagcac ctgcgccaga tcgagcgccg gctcggcatc accatcgcca
     3421 accgcggcgg gcatttcagc ctcaacggtc ccgaggccgc gacgcggatc ggtgccgcgg
     3481 tcatcgaggc gctgtaccgc gctgccggcg aggaggagct gaacccggcc cgggtgcacc
     3541 tgttcctgca ggaggccggc gccgacgaca tcgagctgcc cgatccggtc gaggccgagc
     3601 ccgaggaggt cgcgatccgc acccggcgcg gcatcgtgcg cgggcgcggc atcaaccagc
     3661 agcgctacct gtacgcgatc cagcgtcacg acatcaattt cggcgtcggt ccggccggta
     3721 ccggcaagac ctatctcgcc gtcgcctgcg cggtcgaggc gctggagcgc gacgaggtgc
     3781 gccggctggt gctggtgcgc ccggcggtcg aggccggcga gcggctcggc ttcctgcccg
     3841 gcgacctggt gcagaaggtc gatccgtatc tcaggccgct gtacgacgcg ctctacgaga
     3901 tgctcggctt cgagcgcgtc gccaagctga tggagcgcag cgtcatcgag attgcgccgc
     3961 tcgcgttcat gcgcgggcgc acgctcaacg acgccttcat catcctcgac gaggcgcaga
     4021 acaccacggt cgaacagatg aagatgttcc tgacccgcat cggcttcggc tcgacggcgg
     4081 tgatcaccgg cgacacctcg caggtcgacc tgccgaaggg cgtgcgctcg ggtctcgcgc
     4141 atgcgctgga ggtgctcgat ggtgtcgacg gcatcagcat gacgcgcttc ctgccgcgcg
     4201 acgtggtccg ccacccgctg gtgcagcgca tcgtcatcgc ctacgagcgc cacgagcaga
     4261 agcagggcgg cagcgcatga gccggctcgc gctcgacctg cagatcgaga ccggcgccgg
     4321 cgcgctgccc ccggcggccg atttccgccg ctgggccgaa gccgcgctgg ccggggcgga
     4381 ttttcacgac ccctgcgaga tgagcgtgcg catcgtcggc atcgccgaga gcgcggcgct
     4441 caacgccggc taccggcaca aggacggccc gaccaacgtg ctgtccttcc cgttcgaaac
     4501 cccgcccggc atcgtgctcg atgccgagct gctcggcgat ctggtcatct gtgccccggt
     4561 cgtcgtgcgc gaggccggcg aacagcacaa gcccgaagcc gcgcactggg ctcacctgac
     4621 cgtgcacggc accctgcacc tgctcggcta cgatcacctc gacgatgccg aagccgaggc
     4681 catggaagcg ctcgaaatcc gcattctggc cggtctcggc tatccgaacc cctacgcaga
     4741 cgacgaacga ccatgaagga agaccgatcc ggcgacagta ccggcacgac cggccgcagc
     4801 tggctggaac gcctcggaca ggccctcgcg ggcggacccg aggatcgaga tgacctgaag
     4861 gacatcctca aggaagcgca ggcgcgcggc atcatcgatg ccgagaccct gtcgatgatg
     4921 gaaggcgcgc tgacggttgc cgagcggcag gtgcgcgacg tcatgatccc gcgcgggcag
     4981 atgatcgtca tgcaggccga ctggccggtc gagcgcctgc tcgccgagat catcgagtcc
     5041 ggccactcgc gcctgccggt gatcggcgag gatcgcgacg aagtgctcgg catcctgctg
     5101 gtcaaggacc tgctgcgctg gttcgcgagc ggtgccggcg agttcgacct gcgcagcttc
     5161 ctgcgcgccc cgaccttcat ccccgagagc aagcgcctcg acgtgctgct gcgcgagttc
     5221 cgcgccaacc gcctgcacat ggcgatcgtc gtcgacgagt acggcggggt cgccggcctc
     5281 gtgaccatcg aggacctgat cgagcagatc gtcggcgaga tcgacgacga gcacgacgag
     5341 gccggcgatg acggcgacat ccgccagatc ggcgagcacg agtacatcgt gcgcgcgctg
     5401 acgccgctcg cggacttcaa cgtccgcttc gacgcgcatt tcgacgagga cgaggccgac
     5461 accgtcggcg gcctggtgct gctcgacctc gaacgcgtgc ccaagcgcgg cgaggtcgtc
     5521 gagatcgagc gcttccgctt ccgcgtgctg cgcgccgaca accgccgcgt gcacctgttc
     5581 gggctgagcg tgctcgaccc ggtggtgccg gtggacgcag cgaacgcctg aggccgggca
     5641 gcggcgcgtg cgcagcgccg ccatgcgctc cgtatgccgt cgcgcccggc gtcagccggg
     5701 ctggtgtcag gaggaaccgt cgtggacgag tggacgcagc ggcgcgcgcg catggtcgag
     5761 gagcagatcg aggcgcgcgg catccacgat ccggacgtgc tcgcggcgat gcgccgcgtg
     5821 ccgcgcgagg acttcgtgcc cgaagcgatg cgcgcatggg cctgcgacga ttcgccgctg
     5881 tcgatcggcg acgggcagac gatctcgcag ccgtacatcg tggcgctgat gatcgccgcg
     5941 gcggcgatcc ggcccggcgg gcgcgtgctc gaagtcggca ccggctcggg ctacgcggcg
     6001 gcggtcatgg ccgaactggc cgcgcatgtc tacaccatcg agcgcatcga agcgctggcc
     6061 gcgcaggcgg ccgggcgctt tgcgcgcctc ggctgcacga acatcgaact gcgcaccggc
     6121 gacggcagcg gcggctggcc cgaggcggcg cccttcgacg cgatcctcgt cgccgcgagc
     6181 ggtccgtgcg tgcccgaggt gctgcgggcg caactggcgc cgggcgggcg gctcgtgatg
     6241 ccggtcgatg acggctggct gcagcggctc gtgtgcgtga cgcgcgaggc agccggttcg
     6301 taccgggaag acttcctgtg cgacgtgcgc ttcgtgccgc tgatcggcgc gcacggctgg
     6361 gaaggggcgt gagccggcgc aggccggagc gacgaaggca ttccggccct gctgcatgga
     6421 ttccccggcg aaattgccgg ggttcgcaag cgcacgcgaa cctgcgggct gccgggcgtc
     6481 agccgcgccg ggccgcttcg atcgccgcga tgtcgatttt gcccatctgc atcatcgcct
     6541 cgaaagcgcg cctggcggcg gcacgatcgg gatcggcgat ggcggccgtc agcgcgcgcg
     6601 gcgtgatctg ccacgacaga ccccatgggt ccctgtacca gccgcaggcg ctttcctgac
     6661 caccattgct gacgatcgcg ttccacagcc gatcggtttc ggcctggtcg tcggttgcga
     6721 cctggaacga gaaggcctcg ctgtgcctga acatcgggcc cccattcagg ccggggcaag
     6781 ggatgccgat caccgtggac tcgaccatca gcacatcgcc ctcctggccc gatggataat
     6841 cgccgggcgc gcgatgcacc gtgcccactg cgctgtccgg gaaggtgccg gcgtagaacc
     6901 ccgggggcag cagggcgcgg gctgacggcg cctcgcgctc cgtcgcggtc cgcccgggcg
     6961 catcggctag actgccgctc ccgccgctgc caccccgttc gtcgtgaccg caccgctcgc
     7021 cgatcctgcc gcttcgcctt ccgccgtttc ctccggttcc acggattccg gtgccccggc
     7081 cgccggtcgc cttgctgccg gccatccgct cgcgctgctg ctggctccga tcgcgggtct
     7141 gctgctgccg ctggcgtttg cgccgtaccg gctctggccg ctcgggctgc tgctgccggc
     7201 cgtgctgctg tggctgatcg acggcgagcg cccgcggcgg gcgctgctga tcggtctgct
     7261 gttcggactc ggcagctacg gccacggcat ctggtggatc tacatcagcc tgcaccgcta
     7321 cggcggcgcg ccgccggcgt tcgcggggct tgcgaccttt ctggtcgtgc tgctgatggc
     7381 ggcctacgtc gcgctgttcg ccgggctgct gaaccggctc gcgcccgcgc ccggcgtgcg
     7441 gcgctggctg ctcgcggcac cggggctgtg ggcgctgctc gaatgggtgc gttcgtgggc
     7501 cttcaccggc tttccgtggc ttgcgctcgg ctatgcgcag gtcgagtcac cgctggccgg
     7561 ctttgcgccg atcctcggca ccttcgggct cggtgcgctc gtgatgctgg tggccggctc
     7621 gctcaggctc gcactcgcgc ggctgcgcag cggtgcgcgc gccgcgcttg cgccgctcgc
     7681 cgtcgcgctg ctcgtgccgg cactcggctt cgggctcggg cgcatcgact ggagcgcgcc
     7741 ggcgggtcgg gtgctggcgg tcggtctcgc gcagggcaac atcgcgcagg agatgaaatg
     7801 gcggcccggc aagctggagg agacgatc
//
LOCUS       DAOQJH010000021         7817 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_5072_length_7817_cov_5.472301, whole genome shotgun sequence.
ACCESSION   DAOQJH010000021 DAOQJH010000000
VERSION     DAOQJH010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 7817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7817
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5072_length_7817_cov_5.472301"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(7..906)
                     /locus_tag="PLQ18_00985"
     CDS             complement(7..906)
                     /locus_tag="PLQ18_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011472671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNE00250.1"
                     /translation="MDRHATLLAFVSVVDAGGFAPAARRLGQSPAAVTRLVAALEARL
                     GVRLLARTTRAVTPTDAGQRYLERARALLAAFDEADEAVQDERSQARGHLVVAAPLLF
                     GRLHVAPLLAGFLADEPRVSAELRLGDHYAQLIEERIDVAVRIGPLADSTLVARPVGS
                     VRRVLVAAPDYLARHGHPQTPAALAAHELIACTALTSAAEWIFGDEARALRVPIAPRL
                     RSNSADAALLYAERGGGITALLSYQVAEAVRAGRLVSVLGSFAPPPLPVHLVYPSARG
                     LPARTRRFIDRVVTQADWQFADL"
     gene            1046..1606
                     /locus_tag="PLQ18_00990"
     CDS             1046..1606
                     /locus_tag="PLQ18_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HNE00251.1"
                     /translation="MNDTSRIPAFPSIEAAPAASRPLLDAVHAAFGAVPNLFRVAAHS
                     PAALEGLLGLHGALGKGAIDAATRERVALAIAEYDGCRYCLAAHAWLGARLAGLDADE
                     IAQNRRGRSHDLRADAALRFARRVAETRGHVGPGDLAAVREAGYGDGELVELVALVAL
                     NVLTNYLNEVAQTDIDFPVPAEPAAA"
     gene            1741..3117
                     /locus_tag="PLQ18_00995"
     CDS             1741..3117
                     /locus_tag="PLQ18_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004700783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbosone dehydrogenase family protein"
                     /protein_id="HNE00252.1"
                     /translation="MPAAPFRPVAGRESRHVPLAGLLLAAVLAAGCGETATLPAEAGY
                     GPAPQLPEPKRTLIPTVNVAEASGWPQGVTPVPAPGLAVQAFADGLAHPRWLYVLPNG
                     DVLVAETDAPPKPEDGQGLKGWIMKWFMKRAGSSTPSANRITLLRDADGDGRPETRTV
                     FLEGLNSPFGMALVGEDFYVANTDALLRFPYRRGDTQIRAAPVKLADLPGGPLNHHWT
                     KNLIADRDGRTLYVTVGSNSNVAENGMAVEENRAAILAIERSSGKTRVYASGLRNPNG
                     LAWQPQTGQLWTVVNERDELGSDLVPDYLTSVQDGGFYGWPYSYYGAHVDARVEPPRP
                     ELVARALQPDYALGAHTASLGLSFYSGTLMSQYRNGAFIGQHGSWNREPPAGYRVVFV
                     PFRDGRPDGAVQEVLGGFRDGDQARGRPVGVAIDAHGALLVADDVGNTVWRVTPAVRP
                     AAAPVATR"
     gene            3284..5542
                     /gene="ppk1"
                     /locus_tag="PLQ18_01000"
     CDS             3284..5542
                     /gene="ppk1"
                     /locus_tag="PLQ18_01000"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012826937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HNE00253.1"
                     /translation="MSVPPLDPLLPADAPAAVEARSANSPILTVAGPAETPAIPAVSN
                     AVPPDLDSPELYLNRELTWLAFNRRVLHEAEDARTPLLERVKFLAIVSSNLDEFFMKR
                     IGGLKQQIAAGVRTPSVDGRMPRQQLSECYAAVREINEQMRVLSGVLHGLLIAESIHV
                     RRCRNLTTEQQADLRQMYIRTVLPLVTPQAMDPAHPFPFISNLSLNLLVTVRYPHDEQ
                     VSLMRVKVPTGQGVERLIALENNTIFVPLEDVMAENLDLLVPGMVIEAVECFRVTRNA
                     VVEADEDQADDLLLLIESELRERRFAPTVRLEVNRGMDPVHRGMLAAELGLDEDTDVF
                     DVGGMMAMRDLFQIAGVDRPELHDTPHGPLDHPKLAGSPNIFHAIRDAGTILLQHPYE
                     SFATSVERFLREAARDPKVLAIKMTLYRTSADARIIQYLVEAARNGKQVAVVVELKAR
                     FDEAANIRWAERMEEAGIHVNYGVVGLKTHAKVIFVVRRDYSGLRRYAHIGTGNYHPG
                     TARLYTDLGLLTADEVIGDDLMELFNYLTTGFTPKRNYRKLLPAPKLLKPALLDRIER
                     EIRLHTTKNPGLIQFKMNALEDADIVRALYRAAQAGVRVDLIVRDTCRLRPGLPGISE
                     SVRVISIVGRFLEHPRLFYFRNGGHEEYFIGSADCMKRNLESRVEVVAPVEHPEARLE
                     LRAILDTQLNERRSAWDMQSDGSYVQRTPSENGETRGTHELLIAAAEARTREATRLKK
                     RRPKAIAKRSSL"
     gene            complement(5796..>6067)
                     /locus_tag="PLQ18_01005"
     CDS             complement(5796..>6067)
                     /locus_tag="PLQ18_01005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE00254.1"
                     /translation="CSSMHLTRASIDLDCTSMHLTRASIDLNRAYIDLNSSCTRVTRS
                     LIDLNRACTRVTRTSIDQEPHMDVRDAGHGRPYVAAGHPQLRRRG"
     assembly_gap    6068..6167
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6641..7090
                     /gene="cynS"
                     /locus_tag="PLQ18_01010"
     CDS             6641..7090
                     /gene="cynS"
                     /locus_tag="PLQ18_01010"
                     /EC_number="4.2.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003298680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008824 - cyanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009636 - response to toxic substance
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanase"
                     /protein_id="HNE00255.1"
                     /translation="MISDRSEVTKMILAAKVRKGIRWAEVAAKIGLSKEWTTAACLGQ
                     MTLDKTQAGIVGEIFELSDEAVAWLQIVPYKGSLPSAVPTDPLIYRWYEIVNVYGTTI
                     KELIHEEFGDGIMSAIDFSMDISREPDPKGDRVHVVLSGKFLPYKSY"
     gene            complement(7205..>7817)
                     /locus_tag="PLQ18_01015"
     CDS             complement(7205..>7817)
                     /locus_tag="PLQ18_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007855058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MOFRL family protein"
                     /protein_id="HNE00256.1"
                     /translation="ITLPPAVRAALEAGELETPKPDDPCFARCEYHLIATPMRSLEAA
                     AAVARAAGVTPLILGDALEGEAAEVAKVHAGIARSIAQHGRPLAAPCVLLSGGETTVT
                     VRGRGRGGRNVEFLLALAVALDGHPRVHALAADTDGVDGAEDIAGALCDPTTLARARA
                     LGIDPKASLADNDGHGFFGALGDALVTGPTDTNVNDFRAILVR"
BASE COUNT         1207 a         2713 c         2705 g         1092 t
ORIGIN      
        1 cagggctcag agatccgcga actgccagtc ggcctgcgtg acgacgcgat cgatgaagcg
       61 ccgggtgcgg gccggcaggc cgcgggcgct cgggtagacc agatgcaccg gcagcggcgg
      121 cggggcgaag ctgccgagca cggacacgag ccggccggcg cgcacggctt cggcgacctg
      181 atacgacagc agcgcggtga tgccgccgcc gcgctcggcg tacaggaggg ctgcatcggc
      241 gctgttgctg cgcaggcgcg gggcgatcgg cacgcgcagc gcccgggcct cgtcgccgaa
      301 gatccattcg gcggcactcg tcagcgccgt gcaggcgatc agttcgtgtg cggccagcgc
      361 ggccggggtc tgcgggtggc cgtgccgcgc cagataatcg ggggcggcga ccagcacgcg
      421 gcgcacgctg ccgaccgggc gtgcaaccag cgtcgaatcg gcgagcgggc cgatgcgcac
      481 ggccacgtcg atgcgctcct cgatcagctg cgcgtagtga tcgccgaggc gcagctcggc
      541 gctgacccgc ggctcatcgg cgaggaagcc agccagcagc ggcgcgacgt gcagccgccc
      601 gaacagcagc ggagcggcga ccacgagatg tccgcgcgcc tggctgcgtt catcctgcac
      661 ggcttcgtcg gcctcgtcga aggccgcgag cagcgcgcgt gcccgctcca gatagcgctg
      721 gccggcgtcg gtcggcgtca ccgcccgcgt cgtgcgcgcc agcaggcgca cgccgagccg
      781 ggcttcgagc gccgcgacca ggcgcgtgac tgcggccggc gactgcccga gccggcgcgc
      841 ggccggggcg aagccgccgg cgtcgacgac gctgacgaag gcaagcagcg tggcatggcg
      901 gtccatggat tcctccgtct gacgcaatga tgtcgtgcag agcaaacgga ttatcgtcac
      961 cggcatcggc aatcacactg gctcggcatc ggcggcgctc ccggccgccg aacctgccga
     1021 gagccatgcc gccatgaacg acacgatgaa cgacacgtcc cgcatccccg ccttcccgtc
     1081 gatcgaggcc gctccggccg cgtcccggcc gctgctcgat gccgtgcacg ctgccttcgg
     1141 tgccgtgccc aatctgtttc gcgtcgccgc gcacagcccg gcggcgctcg aaggcctgct
     1201 cggcctgcac ggtgcgctcg gcaagggcgc gatcgacgcc gcgacccgcg agcgcgtggc
     1261 actcgccatc gccgaatacg atggctgccg ctactgcctg gccgcgcacg cctggctcgg
     1321 tgcccggctc gccgggctcg atgccgacga gatcgcgcag aaccgccgcg gccgctcgca
     1381 cgacctgcgc gccgatgcgg cgctgcgctt cgcgcgccgc gtcgccgaga cccgcggcca
     1441 cgtcggcccc ggcgatctgg ccgcggtgcg cgaggccggc tacggcgatg gcgagctggt
     1501 cgaactcgtg gcgctggtcg cgctgaacgt gctgaccaac tacctgaacg aggtcgcgca
     1561 gaccgacatc gacttcccgg tgccggcgga accggcggcg gcctgaaagc cggcaggacc
     1621 cggcgctgcg ctgcgcgccg gtcccgttcc ggggcatgag caaacgttca tgccccggaa
     1681 cggggccggc ggccggccgg tcgcacagcg gcaatccccc cgtgccgccg gagtccgccg
     1741 atgcctgccg ccccgtttcg ccccgtcgcc ggccgcgagt cgcggcatgt gccgctcgcc
     1801 ggcctgctgc tggctgccgt cctcgccgcc ggctgcggcg agaccgcgac gctgccggcc
     1861 gaggccggct acggaccggc accgcagctc ccagagccga agcgcacgct gatcccgacc
     1921 gtcaacgtcg ccgaggccag cggctggccg cagggcgtga cgccggtgcc ggcgccgggg
     1981 ctcgccgtgc aggccttcgc cgacgggctc gcgcatccgc gctggctgta cgtgctgccg
     2041 aacggcgacg tgctggtcgc cgagaccgac gccccgccga agcccgagga cggccagggg
     2101 ctgaagggct ggatcatgaa atggttcatg aagcgcgccg gcagcagcac gccgagcgcc
     2161 aaccgcatca cgctgctgcg cgatgccgac ggcgacggcc ggccggaaac gcgcacggtg
     2221 ttcctggagg gactgaactc gccgttcggc atggcgctgg tcggcgagga tttctatgtc
     2281 gccaacaccg atgcgctgct gagattcccg taccggcgcg gcgacacgca gatccgcgcc
     2341 gcaccggtga agctcgccga cctgccgggc gggccgctga accatcactg gaccaagaac
     2401 ctcatcgccg accgggacgg ccgcacgctg tacgtgacgg tcggctccaa cagcaacgtc
     2461 gccgagaacg gcatggcggt cgaggagaac cgcgcggcga tcctcgcgat cgagcgcagc
     2521 agcggcaaaa cccgcgtcta cgcctcgggc ctgcgcaacc ccaacggcct ggcctggcag
     2581 ccgcagaccg ggcagctgtg gaccgtcgtc aacgagcgcg acgaactcgg cagcgatctg
     2641 gtgccggatt acctgacctc ggtgcaggac ggcggcttct acggctggcc gtacagttac
     2701 tacggcgcgc acgtcgatgc ccgcgtcgag ccgccgcgcc cggagctggt cgcacgggcg
     2761 ctgcagcccg attacgcgct cggtgcgcac accgcctcgc tcggcctgag cttctattca
     2821 ggcacgctga tgtcgcagta ccgcaacggc gccttcatcg gtcagcacgg ttcgtggaac
     2881 cgcgagccgc cggccggtta tcgcgtcgtg ttcgtgccgt ttcgcgacgg ccggcccgac
     2941 ggcgccgtgc aggaggtgct cggcggcttt cgcgacggcg atcaggcgcg cggccggccg
     3001 gtcggggtcg cgatcgacgc gcacggggcg ctgctggtcg ccgatgatgt cggcaacacc
     3061 gtctggcgcg tgacgccggc ggtcaggccg gcggcagccc cggtggcgac gcgctgaagc
     3121 cgcgcgacat ccgtaatcaa accgtcatgc gactgtcgtc aaagcgtcgc aaaatgggca
     3181 gacgccgtct gtcggtgcgt gcgcgtgcgg ttgccggctt cgagtccgga tgcaccggtc
     3241 accggcggcg gcggccgtcg cgagccaccg gagttcccgc acgatgtccg ttccaccact
     3301 cgacccgctg ctgccggccg acgcgccggc agccgtcgag gcgcgcagcg ccaattcccc
     3361 gatcctcacg gttgccggtc cggccgaaac cccggccatt cccgccgtca gcaacgccgt
     3421 accgcccgat ctcgacagcc ccgagctgta cctgaaccgc gagctgacct ggctggcgtt
     3481 caaccgccgc gtgctgcacg aggccgagga cgcgcgcacg ccgctgctcg aacgcgtgaa
     3541 gttcctcgcg atcgtcagct ccaacctcga tgaattcttc atgaagcgca tcggcggcct
     3601 gaagcagcag atcgccgccg gcgtgcgcac gccgagcgtc gacgggcgca tgccgcgcca
     3661 gcagctcagc gagtgctacg cggccgtgcg cgagatcaac gagcagatgc gcgtgctctc
     3721 cggcgtgctg cacggactgc tgatcgccga gagcatccac gtgcggcgct gtcgcaacct
     3781 cacgaccgag cagcaggcgg atctgcgcca gatgtacatc cgcacggtgc tgccgctggt
     3841 gacgccgcag gcgatggacc cggcgcatcc gttcccgttc atctccaacc tgtcgctgaa
     3901 cctgctggtg acggtgcgct acccgcacga cgagcaggtg tcgctgatgc gcgtgaaggt
     3961 gccgaccggc cagggcgtcg agcggctgat cgccctggag aacaacacga tcttcgtgcc
     4021 gctcgaggac gtgatggccg agaacctcga cctgctggtg ccgggcatgg tcatcgaggc
     4081 ggtcgagtgc ttccgcgtca cgcgcaacgc cgtggtcgag gccgacgagg atcaggccga
     4141 cgacctgctg ctgctgatcg aatccgagct gcgcgagcgg cgcttcgcgc cgaccgtgcg
     4201 cctggaggtc aaccgcggca tggacccggt gcaccgcggc atgctcgccg ccgaactcgg
     4261 tctcgacgag gacaccgacg tgttcgatgt cggcggcatg atggcgatgc gcgacctgtt
     4321 ccagatcgcc ggcgtcgacc gccccgaact gcacgacacg ccgcacggtc cgctcgacca
     4381 ccctaagctc gccggctcgc cgaacatctt ccacgccatc cgcgacgccg gcaccatcct
     4441 gctgcaacac ccctacgagt cgttcgcgac ctcggtcgaa cgcttcctgc gcgaggcggc
     4501 gcgcgatccg aaggtgctgg cgatcaagat gacgctgtac cgcacctcgg ccgatgcgcg
     4561 gatcatccag tacctggtcg aggccgcgcg caacggcaag caggtggcgg tggtcgtcga
     4621 gctgaaggcg cgcttcgacg aggccgccaa catccgctgg gcggagcgca tggaagaggc
     4681 cggcatccac gtcaactacg gcgtcgtcgg cctcaagacc cacgccaagg tcatcttcgt
     4741 cgtgcgccgc gactactcgg gcctgcgccg ctacgcgcac atcggcactg gcaactacca
     4801 cccgggcacg gcgcggctgt acaccgacct cggcctgctg accgccgacg aggtgatcgg
     4861 cgatgacctg atggagctgt tcaactacct gaccaccggc ttcacgccca agcgcaacta
     4921 ccgcaagctc ctgccggcgc cgaagctgct gaagcccgcg ctgctcgatc gcatcgagcg
     4981 cgagatccgc ctgcacacca cgaagaaccc cggcctgatc cagttcaaga tgaacgcgct
     5041 cgaggacgcc gacatcgtgc gtgcgctgta ccgcgccgcg caggccggcg tgcgcgtcga
     5101 cctgatcgtg cgcgacacct gccggctgcg tcccggcctg ccggggattt cggagagcgt
     5161 gcgcgtgatc agcatcgtcg ggcgcttcct cgaacacccg cggctgttct acttccgcaa
     5221 cggcggccat gaggaatact tcatcggctc ggccgactgc atgaagcgca acctcgaaag
     5281 ccgcgtcgag gtcgtcgcgc cggtcgagca cccggaggcg cggctggagc tgcgcgcgat
     5341 cctcgacacc cagctcaacg agcggcgcag cgcctgggac atgcagtccg acggttccta
     5401 cgtgcagcgc acgccgtccg agaacggcga gacgcgcggc acgcacgaac tgctgatcgc
     5461 cgccgccgag gcacgcacgc gcgaagccac gcgtctgaag aagcgccggc cgaaggcgat
     5521 cgccaagcgc agcagcctgt aatccggcat cggcacacgg tttcgcccct gtctccgccc
     5581 cgctttccgc aagggaagcg gggcggcggc gtttgtgcgc ggctggcgtc ctgatccggt
     5641 ggcgacgccg gggagggtcg cggggggcgc agaccgggcc gaaggcagcg cagccgtcat
     5701 ttcgcgtgtt tctgggtgat tgcagggctg ttccggccgg ttgttccggg aatcctgcaa
     5761 tgtgagcttg gtttcctgcg cttgccatgc acgcatcagc ccctcctgcg aagctgtgga
     5821 tgcccggccg caacgtatgg ccggccgtga ccggcatcac gcacgtccat gtgcggttcc
     5881 tgatcgatgg aagtacgggt cacgcgtgtg caagcgcggt tcaggtcgat caaactgcgg
     5941 gtcacacgcg tgcaactgct gttcaggtcg atataagcac ggttcagatc gatggatgca
     6001 cgggtcaagt gcatggaagt gcagtccaga tcgatggatg cacgggtcaa gtgcatggaa
     6061 ctgcagtnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnatg gaagtgcagt
     6181 ccagatcgat ggatgcacgg gtcaagtgca tggaactgca gtccagatcg ataggcgcac
     6241 gggtcaggtg catggaagtg cagtccagat caatggatgc acgggtcagg tgcatggaac
     6301 tgcagtccag gacgatcgaa gcatggggca cacgggcgca agtgcggttc aagtcgatta
     6361 aaccgcggct cacacgcccg caagtgcgca tcgccgtgtc cggtgccgga ctgcgccggc
     6421 tgccgccggc gcctcgaccg gcgcctgccg atcgccgtgc aaggctgcgc cttgcggccg
     6481 cctgccgcgc gtgggtcagg cgtcatggga agaaaaaacc gcgcatctgc gtgtgatcgc
     6541 cggcggccgt gccagtcttc gccgggtgcc cgccgcaatg cagcacggcg gcgggccgtg
     6601 cctggccatc cccgtcctgt caccccggag tccgtcgccg atgatcagcg atcgcagtga
     6661 agtcacgaag atgatcctgg ccgccaaggt ccgcaaaggc atccgctggg ccgaagtggc
     6721 cgcaaaaatc ggcctcagca aggaatggac gacggccgcc tgcctcggcc agatgacgct
     6781 cgacaagacc caggccggca tcgtcggcga gatcttcgaa ctgtccgacg aggcggtcgc
     6841 gtggctgcag atcgtgccgt acaagggctc gctgccgagc gcggtgccga ccgatccgct
     6901 gatctatcgc tggtacgaga tcgtcaacgt ctacggcacg accatcaagg aactgatcca
     6961 cgaggaattc ggcgacggca tcatgagtgc gatcgacttc tcgatggaca tctcgcgcga
     7021 acccgacccc aagggcgacc gcgtgcacgt cgtgctgtcg ggcaagttcc tgccgtacaa
     7081 gagctactga gcccgatccc ggcgcaacag ccggtccttt ccgccccttc cccctggcgg
     7141 gggagggtct ggggagaggg cggccaacca cgcattcgcg cgggatcggc atgcggcccg
     7201 tggctcagcg caccaggatc gcgcggaagt cgttgacgtt ggtgtcggtc gggccggtga
     7261 cgagcgcatc gccgagcgcg ccgaagaagc cgtggccgtc gttgtcggcg aggctcgcct
     7321 tcgggtcgat gccgagcgcg cgggcgcggg cgagggtggt cgggtcgcac agcgcaccgg
     7381 cgatgtcctc ggcaccgtcg acgccgtcgg tgtcggcggc gagcgcgtgc acgcgcggat
     7441 ggccgtcgag cgcgacggcg agcgcgagca ggaactcgac gttgcgcccg ccgcgcccgc
     7501 gtccgcgcac ggtgaccgtg gtctcgccgc ccgagagcag cacgcagggc gcggcgagcg
     7561 gccggccgtg ctgcgcgatc gagcgggcaa tgccggcgtg caccttcgcg acctcggcgg
     7621 cctcgccttc gagcgcatcg ccgaggatca gcggcgtgac gccggcggcg cgcgccaccg
     7681 ccgcggcggc ttcgagcgag cgcatcggcg tggcgatcag gtggtactcg cagcgcgcga
     7741 agcagggatc gtccggcttc ggcgtttcca gctcgccggc ttcgagtgcc gcgcgcacgg
     7801 ccggcggcag ggtgatg
//
LOCUS       DAOQJH010000022         7606 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_5264_length_7606_cov_3.635280, whole genome shotgun sequence.
ACCESSION   DAOQJH010000022 DAOQJH010000000
VERSION     DAOQJH010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 7606)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7606)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7606
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5264_length_7606_cov_3.635280"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..835
                     /locus_tag="PLQ18_01020"
     CDS             <1..835
                     /locus_tag="PLQ18_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNE00257.1"
                     /translation="GIYPVGMKIAAGWYASGLGRALGWLVGALVVGTAFPHLLRGLGA
                     ALPWAGVLAGVSACAALGGLAMYWLVPDGPQLKRGARFDPGAVRTIFAGRELRSAAFG
                     YFGHMWELYTWWAFLPAIVAAWGVQHGTTPAVSLWSFAAIAAGALGCIIGGELSPRWG
                     SARVAQVQLAVSCACCLLAPLAFGLPGPLFFAYLLVWGITVVGDSPQFSTLTARAAPP
                     AYLGSALTWVTAIGFGITVVSIQLFGSLVQHWPIAWLLPLLALGPLPGLLAIRPLAGH
                     R"
     gene            complement(915..1142)
                     /locus_tag="PLQ18_01025"
     CDS             complement(915..1142)
                     /locus_tag="PLQ18_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008607378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2789 domain-containing protein"
                     /protein_id="HNE00258.1"
                     /translation="MDTQTHNLSTLFAQLGLPEDPAGIDSFIREHPLPPDMLVADAPF
                     WNQTQAKFLHDALREDSDWAEMVDELNVRLH"
     gene            1331..1972
                     /gene="pdxH"
                     /locus_tag="PLQ18_01030"
     CDS             1331..1972
                     /gene="pdxH"
                     /locus_tag="PLQ18_01030"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006162981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HNE00259.1"
                     /translation="MTTPLADLRRNYTRGTLDVTEVAADPLAQFGRWFEEAVAAQVPE
                     PNAMTLATVAPDGRPQARVMLLKACDAEGFTFFTNYGSHKAAALAAHPQAALCFWWIE
                     LERQVRVEGRIEPLPRADSAAYFAVRPRDSQIGAWASRQSQPVADGVALEAQFAEQAA
                     RWDGREVELPQHWGGYRLHPGRVEFWQGRPGRMHDRIVYVRTGDAWQIGRLQP"
     gene            2001..3344
                     /locus_tag="PLQ18_01035"
     CDS             2001..3344
                     /locus_tag="PLQ18_01035"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HNE00260.1"
                     /translation="MLLPERWIAACVPRPTDTATHASVRRLGRILIAVFEAVFDGHLL
                     DRAASLAYTTLLSLVPLLAVSFSVLQAFGAKDVLEPLLRDALTPLGGQAALVGDRILE
                     FVGKMQVGVLGSLGIAMLLYTVITTVHKIELALNAIWDVAEPRRPMRRIADYLSVTFA
                     GPVLAFGAIGLTDTVRGAFAGLAGRGYLTLADLGGALLALVPHALGMAAFALLYGLLP
                     NTRVRPGAAIAGSLFAVTVWSLAGRLFASLLSDSNNYSAIYSGFAGAVLFILWLNVNW
                     MIVLLGATVSCAWQAPGALNAGKPDGHAAREALALALMRELGRIHREGLPPPIADELA
                     ERLCARQRAIRDLLARLREDGLIIESDACGWLPARDNAHLEVRAILASVRGPLPAAAA
                     GATLLAPGERALTDCYTGCTLDRLIDLPDDAAPPAPGIESRPAARPNVRPRLGKA"
     gene            complement(3490..4047)
                     /locus_tag="PLQ18_01040"
     CDS             complement(3490..4047)
                     /locus_tag="PLQ18_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002691223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HNE00261.1"
                     /translation="MNIKDELVRHYHWLRVYGLNDSHSGNASVREDDTVWVTPTGCCA
                     DLLSSGDLVACDFEGRIGRGASLDARLHVAVYGSNPRARAILHSHGPYTVAMTMDNEE
                     FVPADFEGQYYFGRVPVITIPFDRYKELAPQKVAEVLAEVPIAVVRGHGVFAAAENIN
                     LAYKWTCSLELSAKTAFIAQQAGKI"
     gene            4328..5620
                     /locus_tag="PLQ18_01045"
     CDS             4328..5620
                     /locus_tag="PLQ18_01045"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNE00262.1"
                     /translation="MSRRLRLWLLIAAALIASVYLLPRWTPELLLGYFRNGEPIDRFT
                     LLLAAVLLMPALFERLHLPGAVGLLVAGVLFGPSVLAIGNPENSTVELFLSIGRVFLM
                     FIAGLEVDLRLFHATRLRSFGYGLATFALPMLAGIGVGWLFDFGLNACFLIGSLLASH
                     TLLGLPIASRLGLARSEVVTVTLGATVFTDVASLLVLAVCISVHTTGFTWLSLLEQLF
                     SLGVYGVLVLIGLPWLGVRFVRRWRHREAPMFLFAVLCLLFAAMGAKAIELEDIVGAF
                     FAGLAVNRVIGHGPVRGRIEFLGATLFIPFFFVIIGYRLDLPVFAATLQTSLPFVATI
                     IAALLLGKLAAALVTGPVYGYARRETLLMWSLSLPQVAATLAVALTAYNTLNADGERL
                     ITETVLNSVIVLMVVTSVLGPLLTERFATTLRHRDARP"
     gene            5617..6186
                     /locus_tag="PLQ18_01050"
     CDS             5617..6186
                     /locus_tag="PLQ18_01050"
                     /inference="COORDINATES: protein motif:HMM:NF016269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSC84-related protein"
                     /protein_id="HNE00263.1"
                     /translation="MNPILFRLLGLLLPLLAVTLPAFAADAPTPAETRAAIRQMRSET
                     LKTLYALEPKARTQVRGAAGYAVFSSTGVHVLFIGGGGGRGVVHDNLTGKDVWMRSAS
                     VGVGLGLGARELNLVLIFRKRDTLKRFMEQGWQFGGEASATARAGSSGSDVTDIEARD
                     GILIYPLSQDGLMARGAIEGTKYWVDPDL"
     gene            6211..6552
                     /locus_tag="PLQ18_01055"
     CDS             6211..6552
                     /locus_tag="PLQ18_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003281455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HNE00264.1"
                     /translation="MASHAGFVEYVCAQAGLGPALGHRKMFGEYALYLDGRVVALICD
                     DQLFLKPSAAGEALLGRAPAHHPYPNARAHFRLDAELDTPGRLQQLLIATAAALPLPP
                     PKKPRRMRRND"
     gene            6578..6982
                     /locus_tag="PLQ18_01060"
     CDS             6578..6982
                     /locus_tag="PLQ18_01060"
                     /inference="COORDINATES: protein motif:HMM:NF023520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3574 domain-containing protein"
                     /protein_id="HNE00265.1"
                     /translation="MIRPIPPCLLLPALLLAGCTSLPPTPCRSGERAVVVETLYFGTA
                     TPDGRVSAADWQDFLAREVTPRFPQGLTVSEASGQWRGAGGRIVQEATHVLTLVTADA
                     DEAALPAIITAYRTRFRQEAVLRVHHAACLAG"
     gene            7042..7404
                     /locus_tag="PLQ18_01065"
     CDS             7042..7404
                     /locus_tag="PLQ18_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003465043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNE00266.1"
                     /translation="MSCSDRLFHAADYLRPSDGEPFRSVVRETAESVIVVWHVRPGQT
                     LPVHVHPHGQDTWTVLAGRADYLAGPGRQVPLAVGDIAVATAGEWHGAINTGDEDFVF
                     VSVVAPGAAGYQRIDGAS"
BASE COUNT          974 a         2748 c         2651 g         1233 t
ORIGIN      
        1 cggcatctac ccggtcggca tgaagatcgc agccggctgg tacgcgagcg gactcgggcg
       61 cgcgctcggc tggctggtcg gtgcgctggt ggtcggcacg gccttcccgc acctgctgcg
      121 gggactcggc gcggcgctgc cctgggccgg cgtgctcgcc ggcgtgtcgg cctgcgcggc
      181 gctcggcgga ctggcgatgt actggctggt gccggacggc ccgcagctga agcgcggggc
      241 gcgcttcgac cccggcgcgg tgcgcacgat cttcgccggc cgcgagctgc gctcggcggc
      301 cttcggctac ttcggtcaca tgtgggagct gtacacctgg tgggccttct tgccggccat
      361 cgtcgccgcc tggggcgtgc agcacggcac cacgcccgcg gtgtcgctct ggagcttcgc
      421 ggcgatcgcc gccggcgcgc tcggctgcat catcggcggc gaactgtcgc cgcgctgggg
      481 cagcgcgcgc gtcgcgcagg tgcagctcgc ggtgtcgtgc gcgtgctgcc tgctcgcgcc
      541 gctcgccttc ggactgccgg ggccgctgtt cttcgcctat ctgctggtct ggggcatcac
      601 cgtcgtcggt gactcgccgc agttctcgac gctgaccgcg cgcgccgcac cgccggcgta
      661 tctcggctcg gcgctgacct gggtgacggc gatcggcttc ggcatcaccg tcgtcagcat
      721 ccagctgttc ggcagcctcg tgcagcactg gccgatcgcc tggctgctgc cgctgctggc
      781 gctcggtccg ctgccgggtc tgctggcgat ccggccgctg gccggacacc gctgaggccc
      841 gcacatgcga cggcccgcag ccgctggctg cgggccgtcg ggacatgcgc cgccgcagcg
      901 gcggctgacc atgctcaatg caggcggacg ttcagttcat cgaccatctc ggcccagtcc
      961 gaatcctcgc gcagcgcatc gtgcaggaac ttcgcctggg tctggttcca gaacggcgca
     1021 tcggccacca gcatgtccgg cggcagcgga tgttcgcgga tgaagctgtc gatgccggcc
     1081 gggtcttccg gcaggccgag ctgggcgaac agggtgctca ggttatgggt ctgggtgtcc
     1141 atcatgtcga actcctcgcg gcagtcatct gggcggtcat ccggactctg ggattacggg
     1201 agcagcctcc cgtacccgga cctttataga ccccgcccgc cgcaaccgtt cctccggccg
     1261 cgcatccggc cgatgcctgc gcggcccttg cctttccgct tcccttctcc atccataagg
     1321 agtgtccgcc gtgaccaccc cgctcgccga tctgcgccgc aactacaccc gcggcacgct
     1381 cgatgtcacc gaagtcgccg ccgatccgct cgcccagttc gggcgctggt tcgaggaggc
     1441 ggtcgccgcg caggtgcccg aaccgaacgc gatgacgctc gccaccgtcg cccccgacgg
     1501 ccggccgcag gcgcgcgtga tgctgctgaa ggcctgcgac gccgaaggct tcacgttctt
     1561 caccaactac ggcagccaca aggccgccgc actcgccgcg catccgcagg cggcgctctg
     1621 cttctggtgg atcgagctgg agcggcaggt gcgcgtcgag ggccgcatcg aacccttgcc
     1681 gcgcgccgac tcggccgcct acttcgccgt gcgcccgcgc gacagccaga tcggcgcctg
     1741 ggcctcgcgc cagagccagc cggtcgccga tggcgtcgcg ctcgaagcgc agtttgcgga
     1801 acaggcggcg cgctgggacg gtcgggaggt tgaactgccg cagcactggg gtggctaccg
     1861 cctgcacccc gggcgcgtcg agttctggca gggccggccg ggacgcatgc acgatcgcat
     1921 cgtctacgtg cgcaccggcg acgcctggca gatcgggcgc ctgcagccct gaagtcgaga
     1981 gcgcggatcg aggaggacgg atgctgctgc ccgaacgctg gatcgctgcc tgtgtgccgc
     2041 ggccgaccga taccgccacg cacgcctcgg ttcgccggct cgggcgcatc ctgatcgcgg
     2101 tcttcgaggc cgtgttcgac ggccacctgc tcgatcgcgc cgccagcctc gcctacacga
     2161 cgctgctgtc gctggtgcca ctgctggcgg tgagcttttc ggtgctgcag gccttcggcg
     2221 ccaaggacgt gctcgaaccg ctgctgcgcg atgcgctgac gccgctcggt ggtcaggcgg
     2281 cgctggtcgg tgaccgcatc ctggagttcg tcggcaagat gcaggtcggc gtgctcggtt
     2341 cgctcggcat cgcgatgctg ctctacacgg tgatcacgac cgtgcacaag atcgagctgg
     2401 cgctgaacgc gatctgggat gtcgccgaac cgcgccggcc gatgcgccgc atcgccgatt
     2461 acctgtcggt gaccttcgcc ggcccggtgc tcgccttcgg cgccatcggc ctgaccgaca
     2521 ccgtgcgcgg cgccttcgcc ggactcgccg ggcgcggcta cctgacgctc gccgatctcg
     2581 gcggcgcgct gctcgcgctg gtgccgcacg ccctcggcat ggcggccttc gcgctgctct
     2641 acggcctgct gccgaacacg cgcgtgcgcc cgggcgcggc gatcgccggc agcctgttcg
     2701 cggtgacggt ctggtcgctg gccgggcggc tgttcgcgag cctgctcagc gattccaaca
     2761 actactcggc gatctactcg ggcttcgccg gcgccgtgct gttcatcctg tggctgaacg
     2821 tcaactggat gatcgtgctg ctcggcgcga cggtgtcctg cgcttggcag gcaccggggg
     2881 cgctgaacgc cggcaagccc gacggccacg ccgcgcgcga ggcgctggcg ctggcgctga
     2941 tgcgcgaact cggccgcatc caccgcgagg gcctgccgcc gccgatcgcc gatgagctcg
     3001 ccgagaggct ctgcgcccgc cagcgcgcga tccgcgacct gctggcgcgg ctgcgcgagg
     3061 acggcctgat catcgagagc gatgcctgcg gctggctgcc ggcgcgcgac aacgcccatc
     3121 tcgaagtgcg cgccatcctc gccagcgtgc gcggtccgct gccggccgcg gccgccggcg
     3181 cgacgctgct cgcgcccggc gaacgcgcgc tgaccgactg ctacaccggc tgcacgctcg
     3241 accggctgat cgacctgccg gatgacgcag caccgccggc acccggcatc gaatcccgcc
     3301 cggccgcccg cccgaacgtg cgcccgcggc tcggcaaggc ctgataccga tcacacgtga
     3361 atgcgttgtt gatccccctc tccccaaccc ctcccccgcg agggggggag gggctgaaaa
     3421 gatcggctgt tcagcagtga ctggtatgag cactgctgcc cgcgacaccc gggcagccgc
     3481 ggaccggcat cagatcttgc cggcctgctg cgcgatgaag gcggtcttcg ccgacagctc
     3541 cagcgagcag gtccacttgt aggcaaggtt gatgttttcg gcggccgcga acacgccgtg
     3601 gccgcgcacg accgcgatcg gcacctcggc cagcacctcg gcgaccttct gcggcgccag
     3661 ctccttgtag cggtcgaacg ggatcgtgat caccggcacg cgcccgaagt agtactggcc
     3721 ctcgaagtcg gccggcacga actcctcgtt gtccatcgtc atcgccaccg tgtacggccc
     3781 gtggctgtgc aggatggcgc gcgcgcgcgg attgctgccg tagacggcga cgtgcagccg
     3841 cgcatcgagc gaggcgccgc ggccgatgcg gccctcgaag tcgcaggcga cgagatcgcc
     3901 gctgctcagc agatcggcgc agcagccggt cggcgtcacc cagaccgtgt cgtcctcgcg
     3961 caccgaggcg ttgccgctgt gcgagtcgtt caggccgtag acgcgcagcc agtgatagtg
     4021 gcgaaccagt tcgtctttga tgttcatcgg gaggaagtcc ggagttcagc ggattcgggc
     4081 gcgcaggcag cgtcgcgacg ggccgcgcgg cgacggcaac agccgcagcg gcggaaacag
     4141 ggggcggcgg caggggccgc ggggggcgct atggtgcggg aaaagccccg ggtgtggaag
     4201 gccggccccg gcagcggtct gaccggcccg gcggtgcggc ggcagcgcag cgacattcgt
     4261 ccatactgcg acgcgcgacc acccggtggt ggccgcaacc ccgaaatccc tccgggagtg
     4321 tctgcgcatg tcgcgtcgtc tgcgtctgtg gctgctgatc gccgccgcac tcattgccag
     4381 cgtgtatctg ctgccacgct ggacccccga gctgctgctc ggatatttcc gcaacggcga
     4441 gccgatcgac cgcttcacgc tgctgctggc cgcggtgctg ctgatgccgg cgctgttcga
     4501 gcgcctgcac ctgcccgggg ctgtcggcct gctggtggcc ggcgtgctgt tcgggccatc
     4561 ggtgctggcg atcggcaatc ccgaaaacag caccgtcgag ttgttcctga gcatcggccg
     4621 cgtgttcctg atgttcatcg ccggactgga ggtcgacctg cggctgttcc acgcgacgcg
     4681 gctgcgctcc ttcggctacg gactcgcgac cttcgcgctg ccgatgctcg ccggcatcgg
     4741 cgtcggctgg ctgttcgact tcgggctgaa cgcctgcttc ctgatcggct cgctgctcgc
     4801 ctcgcacacg ctgctcggcc tgccgatcgc gagccgcctc ggcctggcgc gcagcgaggt
     4861 ggtcaccgtc acgctcggcg ccacggtgtt caccgatgtc gcctcgctgc tggtgctggc
     4921 cgtgtgcatc tcggtgcaca cgaccggctt cacgtggctg agcctgctgg agcagctgtt
     4981 ctcgctcggc gtctacggcg tgctggtgct gatcggcctg ccgtggctcg gcgtgcgctt
     5041 cgtgcgccgc tggcgccatc gcgaggcgcc gatgttcctg tttgcggtgc tctgcctgct
     5101 gttcgcggcg atgggcgcca aggccatcga gctggaggac atcgtcggtg ccttcttcgc
     5161 cgggctggcg gtcaaccgcg tgatcggcca cggccccgtg cgcggcagga tcgaattcct
     5221 cggcgcgacg ctgttcatcc cgttcttctt cgtgatcatc ggctaccggc tcgacctgcc
     5281 ggtgttcgcg gcgacgctgc agaccagcct gccgttcgtc gccacgatca tcgccgcgct
     5341 gctgctcggc aagctcgccg ccgcgctggt gaccgggccg gtgtacggct atgcgcgccg
     5401 cgagacgctg ctgatgtggt cgctgtcgct gccgcaggtg gccgcgacgc tggccgtggc
     5461 gctgacggcc tacaacacgc tgaatgccga cggcgaacgg ctgatcaccg aaacggtgct
     5521 gaacagcgtc atcgtgctga tggtcgtgac ctcggtgctc ggtccgctgc tgaccgagcg
     5581 cttcgccacc acgctgcgcc accgggatgc ccgcccatga atccgatcct tttccgactg
     5641 ctgggcctgc tgctgccgct gctcgccgtg acgctgccgg ccttcgctgc cgatgcaccg
     5701 acgccggccg aaacccgggc cgcgatccgc cagatgcgca gcgaaaccct gaaaaccctg
     5761 tacgcgctgg agccgaaggc acgcacgcag gtgcgcggcg cggccggcta cgcggtgttc
     5821 agcagcaccg gcgtgcacgt gctgttcatc ggcggcggcg gcgggcgcgg cgtcgtacac
     5881 gacaacctga ccggcaagga cgtctggatg cgctcggcca gcgtcggcgt cggcctcggg
     5941 ctcggtgccc gggagctgaa cctggtgctg atcttccgca agcgcgacac gctgaaacgc
     6001 ttcatggagc agggctggca gttcggcggc gaggcgagcg ccacggcccg cgccggcagc
     6061 agcggcagcg atgtgaccga tatcgaagcc cgcgacggca tcctgatcta cccgctcagc
     6121 caggacgggc tgatggcccg aggcgcgatc gagggtacga agtactgggt cgatccggat
     6181 ctctgaaccc gcagcccgga ggacgccccc atggcctcgc acgccggttt cgtcgaatac
     6241 gtctgcgcgc aggccgggct cgggccggcg ctgggccatc gcaagatgtt cggcgaatac
     6301 gcgctctatc ttgacggccg ggtcgtcgcg ctgatctgcg acgaccagct gttcctgaag
     6361 ccgagcgccg ccggcgaggc gctgctcggg cgcgcgccgg cacaccatcc ctacccgaat
     6421 gccagggcgc atttccggct cgacgccgaa ctcgacacgc ccgggcgcct gcagcaactg
     6481 ctgatcgcga ccgccgcggc cctgccgctg ccgcccccga agaaaccgcg ccggatgcgc
     6541 cggaacgact gagcgacgga gcgacggagc gatgcgaatg atcagaccga tcccgccctg
     6601 cctgctgctg ccggcgctgc tgctggccgg ctgcacgtcg ctgccgccga ccccgtgtcg
     6661 cagcggcgag cgcgccgtcg tcgtcgagac gctgtacttc ggcaccgcga ccccggacgg
     6721 gcgcgtgtcg gccgccgact ggcaggactt cctggcccgc gaggtcacgc cgcgctttcc
     6781 gcaggggctg acggtcagcg aggcgtccgg tcagtggcgc ggcgccgggg ggcgcatcgt
     6841 ccaggaggcc acgcacgtgc tgacgctggt cacggccgac gccgacgagg ccgcgctgcc
     6901 ggccatcatc acggcctacc gcacgcgctt ccggcaggag gccgtgctgc gcgtgcacca
     6961 cgccgcctgc ctcgccggct gaagccggtg cgaaacctgc atcgccgcca gcgcccttcc
     7021 cccagccgtg gagtcccatc gatgagttgc agcgaccgcc tgttccacgc cgccgactat
     7081 ctgcgcccga gcgacggcga gccgttccgg tcggtcgtgc gcgagaccgc cgagtcggtg
     7141 atcgtcgtct ggcacgtgcg ccccggtcag acgctgccgg tccacgtcca cccgcacggt
     7201 caggacacct ggaccgtgct cgccggccgt gccgattacc tcgccggtcc cggccggcaa
     7261 gtgccgcttg ccgtcggcga catcgccgtc gccacggccg gcgagtggca tggcgccatc
     7321 aataccggtg acgaggactt cgtgttcgtg tcggtggtag cccccggtgc ggccggatat
     7381 cagcgcatcg atggcgcatc gtgagcgcag cttccggcct cctgcgcccg gactgaacca
     7441 cgctgccgcc ggctgccggt gcattgccgc cattgcaact ggtgcaacgc accatccgga
     7501 ggcaggcgcg taatgccggc agcccggcat tcgggccggc cgccgtttcg agggcagggc
     7561 tgtgcaacgc tcccctggta gtgcaaagtg tcgcgccctc acagcc
//
LOCUS       DAOQJH010000023         7600 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_5277_length_7600_cov_8.374553, whole genome shotgun sequence.
ACCESSION   DAOQJH010000023 DAOQJH010000000
VERSION     DAOQJH010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 7600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7600
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5277_length_7600_cov_8.374553"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="PLQ18_01070"
     CDS             <1..213
                     /locus_tag="PLQ18_01070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE00267.1"
                     /translation="ALIDQPPALIDRPPTLIDRPPALIDRPPALIDRPPALIDRPPAL
                     IDRPPALIFPSRIGIARPPPGGHDGR"
     gene            complement(251..718)
                     /locus_tag="PLQ18_01075"
     CDS             complement(251..718)
                     /locus_tag="PLQ18_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE00268.1"
                     /translation="MPVNRINATLSDEQRRQALAALASLNDALPFLIDLSADERNSMA
                     KFGDKNRSFVVKALAIAENHPEILPRSLDLDELRADVELVESLYPLRNAVEALLGRID
                     NTYFAAGSEAYVSALLVYQYAKTHNVATGALEESLDDLGRRFSRRNRKSGEGQ"
     gene            complement(826..1713)
                     /locus_tag="PLQ18_01080"
     CDS             complement(826..1713)
                     /locus_tag="PLQ18_01080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE00269.1"
                     /translation="MNASPPPPAARPASLALKIALAGVAFAVILLESWIADGLKGDEL
                     FPWWPGGKLKYGDTAFSCAVGGMIGLAALGIGFLYRQGRRLLPVEVRRIGRPGHVRPR
                     AVLVLPVSTPRWQWSPSGLDGGPDGKTWPLPPTLPAALAEMARLAAPGDGKTLPSFAW
                     EQLLRAIAPHADRLQRIVLIGSPGERGSFATLPRCRQMIRHYFPSLDAIAIDEREASF
                     DALDELLDCYREIIAGEAARKSEIMLDVTGGTKVVSIVAAIVTVEHPEIEFQYVDTEG
                     EKEVRSFNIVSAAEPPAAA"
     gene            complement(1710..>2647)
                     /gene="cmr6"
                     /locus_tag="PLQ18_01085"
     CDS             complement(1710..>2647)
                     /gene="cmr6"
                     /locus_tag="PLQ18_01085"
                     /inference="COORDINATES: protein motif:HMM:TIGR01898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type III-B CRISPR module RAMP protein Cmr6"
                     /protein_id="HNE00270.1"
                     /translation="LRQHQPHCGLLLARGLVALPTTQTSAAGSGGSDGSDGSDGSDGS
                     DGSNGSDGSGGSGGSGGTGQPAAGECKARHIAAVVAAANVARGGALYAAAYDRWCRLT
                     ADDRRFAVLDAPLAGRLYIGVARDNALETGVSLSHTYGMPFIPGSAVKGLCRAAAGEW
                     LAQREARRYLFGDDRRPRAANDARDAAADAADADPAEAGIDSEIGGVIFHDAWWNPAG
                     KAPPLVAEVVTGHHAGYYGSAGKQDATDFDAPVPAPQIAVSGRFRFVIEGDPAWTRLA
                     CRLLAGGLQQRGIGGKRSSGYGHFLLNPSLPTDRA"
     assembly_gap    2648..2747
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2857..3333)
                     /locus_tag="PLQ18_01090"
     CDS             complement(2857..3333)
                     /locus_tag="PLQ18_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003045289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III-B CRISPR module-associated protein
                     Cmr5"
                     /protein_id="HNE00271.1"
                     /translation="MSPAPRTRAQRLAEAALTRIRAVAADWPADGQSRPSAGSSAGFS
                     RAQYKARADQFPVLVMQAGLAQALGFLRAKAGDAGGGDNAYRRYLNDLAVVVGASDGE
                     ALQQQAIAAPLPAYRQLTRQVLDAAGWLKRFGQAYLGASADAGNGAPTAQEAPWTP"
     gene            complement(3330..4355)
                     /gene="cmr4"
                     /locus_tag="PLQ18_01095"
     CDS             complement(3330..4355)
                     /gene="cmr4"
                     /locus_tag="PLQ18_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003778861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-B CRISPR module RAMP protein Cmr4"
                     /protein_id="HNE00272.1"
                     /translation="MLTQIFHLHALSALHVGSGQALGAVDLPVARARATHLPIVPGSA
                     LKGVLRDEFAADERQQKTLLTLFGPPSIDSGDAAYAGALAFGDAQLLLLPVRSLAGIV
                     AFVTCPFVLARYAEDLRRETLAAPTAAAAGGAVGVVRAAPTVRDPPDVPPVDEQQALV
                     CQSTKIIAQHKPGTLVVLEDLDLRASGQNAGPHGAAPGADAWAERIAARVHPGDKKWQ
                     ALLGERFAIISDADFSFLADTATEVRARIRIDDSRGVVDNGALWYEENLPAETVLWGQ
                     IAAGPARDGQPLAAAQTLAAFRAGLAGNGHGALTLQIGGKATIGRGLVRFVLPPAADK
                     AAGGDKR"
     gene            complement(4349..>5427)
                     /locus_tag="PLQ18_01100"
     CDS             complement(4349..>5427)
                     /locus_tag="PLQ18_01100"
                     /inference="COORDINATES: protein motif:HMM:NF021236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III-B CRISPR module-associated Cmr3 family
                     protein"
                     /protein_id="HNE00273.1"
                     /translation="GDTGDTGDTGAPPPWPGTVDREQQRALRALAAHGPLLAHCCAGK
                     ITPMLPRPADALLLADAHGAGAYQRLQPGALPAGCGCDLPTGLRPLLLPPGAGKGKVQ
                     PGPRFWPLSTLLAWRRGASVALPAHDEPPSETRTHVALARNTLAADPGRLFQSDGLDF
                     SPRRRADRHGFTVEQWIFLARFAQDIAPRWVTFGGERRLSWLAAAPREVLAAPPEHLA
                     ELAGARRLTLSLATPALFAAGWRPAWLNDDLCGCPPACPDLQLRLVAAAVDGWLSVSG
                     WDLVAAGDGAGRPTAGAKAARKAVPAGATYWFEVIGAPPDDWAERLWLAPIADDEQDR
                     RDGFGLVIPGLWPTPQQQGEESAC"
     assembly_gap    5428..5527
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5528..5680)
                     /locus_tag="PLQ18_01105"
     CDS             complement(<5528..5680)
                     /locus_tag="PLQ18_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015258280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III-B CRISPR module-associated Cmr3 family
                     protein"
                     /protein_id="HNE00274.1"
                     /translation="MYDYLIVPRAPLVFRSGRPFGVGGRDAAHFPWPSSLAGLLRTQV
                     LDDRSEA"
     gene            complement(5734..>7600)
                     /locus_tag="PLQ18_01110"
     CDS             complement(5734..>7600)
                     /locus_tag="PLQ18_01110"
                     /inference="COORDINATES: protein motif:HMM:NF023885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III-B CRISPR-associated protein Cas10/Cmr2"
                     /protein_id="HNE00275.1"
                     /translation="MSTWLLEIAIGPVQGFIAAARRSRDLWAGSHLLSELVGATARHL
                     LDNGAQLIYPLAARVRRASPDENSNLSNILLAKVEAADEAAVRELARAAQDKARAHLR
                     VFADNALDDWQRGSNGARIRLRQDFWTRQVEDAIDSYAAWARVAEAADPSGDGRTPYR
                     IAYDKLKGALAARKHTRDFAPMFPPAASDKGAGIPKCSFDGLRESVLPELPAGESFPA
                     RFGLARGEQLDALGCIKRVVGRNERFTALSRFAAHGWLSGLDDKSRSTLCAAYKKLLD
                     PALNLATGALDTAHADFPYDGGLLFPERIAVAEQERRAEPAALAALAELRRTLRPIWR
                     DHGQPCPYAVLVIADGDRMGRLVDRARSAEEHERISRAVAAFADRVPGIAREVAGQCV
                     FAGGDDLTLYFPLAGVVHGAFDLHDAFTEALAEVVGALPPPADGAPPPTLRVGAAICH
                     VLEPLAVIRQRAADAERLAKGEGGPAAPGNALGLLLSLRSGPQVGLRLSFADAHGRAA
                     LDHWQAAYAAHRLPGRLAFDTRAIGLHCRAIGAGAGVAEGEFLRLLARARQGGGGERI
                     DHATRNLLAERQAALAPLAAADDPGALHRLGDELILARWLAARSAAEIGAFLESH"
BASE COUNT          952 a         2419 c         2930 g         1099 t
ORIGIN      
        1 gcattgatcg atcaacctcc ggcattgatc gatcgacctc cgacattgat cgatcgacct
       61 ccagcattga tcgatcgacc tccggcattg atcgatcgac ctccggcatt gatcgatcga
      121 cctccggcat tgatcgatcg acctccggca ttgatctttc cttcccgcat cgggattgcc
      181 cgcccgccgc cgggcggcca cgacgggcgg tgaagcgccg gccggcagag cgtcgggcgg
      241 cgcggggggg tcattgacct tcgccgctct tgcgattgcg ccgcgagaag cgccggccga
      301 ggtcgtcgag gctttcttcc agcgcgccgg tggcgacgtt gtgcgttttg gcgtactggt
      361 agacgagcag cgcgctgacg taggcttcgc tgccggcggc gaagtaggtg ttgtcgatgc
      421 gacccaacag ggcttcgacc gcgttgcgca gcgggtagag gctttcgacg agttcgacgt
      481 cggcgcgtaa ttcgtccagg tcgaggctgc gcggcaggat ttcggggtgg ttctcggcga
      541 tggccagcgc cttgacgacg aagctgcggt tcttgtcgcc gaacttggcc atcgagttgc
      601 gttcgtcggc gctgaggtcg atcaggaagg gcagtgcatc gttcaggctg gccagtgcgg
      661 cgagggcctg gcggcgctgt tcgtcgctca gcgtggcgtt gatgcggttg acgggcatgg
      721 cttttctcct tttcgcagag ggtggatgag tttgcgcaaa tggccttgaa ttattgtggc
      781 atttgccgcc cgtcgtcaat ctggcggggg ccgccggcgc ccgcttcagg ctgcggccgg
      841 cggttcagcg gcggagacga tgttgaagct gcgcacctct ttttcgcctt cggtatcgac
      901 gtactggaac tcgatctccg ggtgttcgac ggtgacgatg gcggcgacga tgctgacgac
      961 tttcgtgccg ccggtgacat cgagcatgat ttcgcttttg cgcgccgctt cgccggcgat
     1021 gatttcgcgg tagcaatcga gcaactcgtc gagcgcgtcg aagctcgcct cgcgttcgtc
     1081 gatggcgatc gcgtccaggc tggggaagta gtggcggatc atctgccggc aacgcggcag
     1141 cgtcgcgaag ctgccgcgct cgccgggcga gccgatgagg acgatccgtt gcaggcgatc
     1201 ggcatgcggc gcgatggcgc gcagcaactg ctcccaggcg aaactgggca gcgtcttgcc
     1261 gtcgccgggt gcggcgagcc tggccatttc ggcgagcgct gccggcaacg tcggcggcag
     1321 cggccaggtc ttgccgtccg ggccgccgtc caggccgctg ggcgaccatt gccagcgcgg
     1381 cgtcgacacc gggaggacga ggacggcgcg cggccggaca tggccgggac ggccgatgcg
     1441 gcgcacttcg actggcagca ggcgccgccc ttgccggtag aggaagccga tgccgagcgc
     1501 agcgaggccg atcatgccgc cgacggcgca ggaaaaggcg gtgtcgccat acttgagctt
     1561 gccgcccggc caccagggga agagttcgtc gcccttgagg ccgtcggcga tccagctttc
     1621 gagcaggatg acggcgaagg cgacgccggc cagggcgatt ttgagggcaa gcgaggcggg
     1681 ccgcgcggcg ggcgggggtg gggaggcgtt catgctcggt ctgtcgggag ggaggggttg
     1741 aggagaaagt ggccgtagcc ggaactgcgc ttgccgccga tgccgcgctg ctgcaggccg
     1801 ccggcgagca ggcggcaggc caggcgcgtc cacgccgggt cgccttcgat gacgaaacgg
     1861 aagcggccgc tgacggcgat ctgcggcgcc ggcaccggcg cgtcgaagtc ggtggcgtcc
     1921 tgcttgccgg cgctgccgta gtagccggcg tgatggccgg tgacgacttc ggcgacgagc
     1981 ggcggcgcct tgccggccgg gttccaccag gcgtcgtgga agatgacgcc gccgatttcg
     2041 ctgtcgattc cggcttcggc cgggtcggcg tcggcggcgt cggcggcggc gtcgcgcgcg
     2101 tcgtttgccg cgcgcggccg gcggtcgtcg ccgaaaaggt agcggcgggc ttcgcgctgc
     2161 gccagccatt cgccggcggc ggcgcggcac agtcccttga ccgccgagcc gggaatgaac
     2221 ggcatgccgt aggtgtgcga caggctgacg ccggtttcca gcgcgttgtc gcgcgcaacg
     2281 ccgatgtaga gccggccggc gagcggcgcg tcgaggacgg cgaagcgccg gtcgtcggcg
     2341 gtcaggcggc accagcggtc gtaggcggcc gcgtaaagcg cgccgccgcg cgccacgttc
     2401 gcggcggcga cgacggcggc gatgtgccgg gctttgcatt cgccggcggc gggctggccg
     2461 gttccgccgc ttccgccgct tccgccgctt ccgtcgcttc cgttgctccc gtcgcttccg
     2521 tcgcttccgt cgcttccgtc gctcccgtcg ctcccgccgc tcccggccgc gctcgtctgc
     2581 gtagtcggca gtgcgaccag cccgcgcgcg aggagcaggc cgcagtgcgg ctggtgttgg
     2641 cgcaacgnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngcg accagcccgc
     2761 gcgcgaggag caggccgcag tgcggctggt gttggcgcaa cgcggcgaag tcgcggtcgg
     2821 ccagcagcgg ctgcaaggca gcgcggcaga gcgggatcat ggcgtccatg gcgcctcctg
     2881 cgcggtcggc gcgccgttcc cggcgtcggc gctcgcgccg agataggctt gcccgaagcg
     2941 cttgagccag ccggcggcgt cgagtacctg gcgggtgagt tgccggtagg cgggcagtgg
     3001 ggcggcgatc gcctgttgct gcagcgcctc gccgtcgctt gcgccgacga cgacggcgag
     3061 atcgttcaga tagcgccggt aggcgttgtc gccgccgccg gcgtcgcccg ccttggcgcg
     3121 cagaaagccg agcgcctgcg ccaatccggc ctgcatgacg aggaccggga actggtcggc
     3181 gcgcgctttg tattgggcgc gtgaaaagcc ggccgaggag ccggccgacg ggcggctctg
     3241 gccgtccgcc ggccaatcgg cggcgacggc gcggattcgg gtcagcgccg cttcggccag
     3301 gcgctgcgcg cgcgtgcgcg gagcggggct catcgtttgt ctccgccggc agccttgtcc
     3361 gccgctggcg gcaggacgaa gcggacgagg ccgcgcccga tcgtcgcctt gccgccgatc
     3421 tgcagcgtca gtgcgccgtg cccgttgccg gccagaccgg cgcggaaggc ggcgagcgtc
     3481 tgcgcggcgg cgagcggctg gccgtcgcgc gccgggccgg cagcgatctg gccccagagg
     3541 acggtctccg ccggcaggtt ttcttcgtac cacagcgcgc cgttgtcgac gacgccacgc
     3601 gagtcgtcga tgcggatgcg cgcgcgtacc tcggtcgccg tgtcggcgag gaatgagaag
     3661 tcggcgtcgg aaatgatggc gaagcgttcg ccgagcagcg cctgccattt tttgtcgccg
     3721 ggatggacgc gggcggcgat ccgttcggcc caggcgtcgg cccccggcgc ggcgccgtgc
     3781 gggccggcgt tctggccgct cgcccgcagg tcgaggtctt cgagcacgac gagtgtgccg
     3841 ggtttgtgct gggcgatgat cttcgtgctt tggcagacga gcgcctgctg ctcgtcgacc
     3901 ggcggaacgt cgggggggtc gcggacggtc ggcgctgcgc ggacgacgcc gactgcgccg
     3961 ccggcggccg ccgccgtcgg cgcggcgagg gtttcgcggc gcaggtcttc ggcgtagcgc
     4021 gcgaggacga aggggcaggt gacgaaagcg acgatgccgg ccagcgagcg caccggcagg
     4081 agcaggagct gcgcgtcgcc gaaagccagc gcgccggcgt aggcggcgtc gccggagtcg
     4141 atgctcggcg gtccgaagag cgtcaggagt gtcttctgct gccgctcgtc ggcggcgaat
     4201 tcgtcgcgca ggacgccttt cagcgccgag ccggggacga tcggcaggtg ggtcgcgcgg
     4261 gcgcgcgcca ccggcaggtc gacggcgccg agcgcctggc cgctgccgac gtgcagcgcg
     4321 ctcagggcgt gcaggtgaaa gatctgggtc agcatgcgct ttcttctcct tgttgttgcg
     4381 gggttggcca gagaccgggg atgaccaggc cgaagccgtc gcggcgatcc tgttcgtcgt
     4441 cggcgatcgg cgccagccag aggcgttctg cccagtcgtc gggtggggcg ccgatgactt
     4501 cgaaccagta ggtggcgccg gcgggaaccg ctttgcgggc ggctttggcg ccggcggtgg
     4561 ggcggccggc gccgtctccg gcggcgacga ggtcccagcc ggagacggag agccagccgt
     4621 cgacggcggc ggcaaccagg cggagctgca ggtcggggca ggcgggcggg cagccgcaaa
     4681 gatcgtcatt cagccaggcg ggccgccagc cggcggcgaa gagcgccggg gtggcaaggc
     4741 tgagcgtcag gcggcgcgcg ccggcgagtt cggcaaggtg ctcgggcggc gcggcgagca
     4801 cttcgcgcgg cgccgcggcg agccacgaca gccgccgttc gccgccaaag gtgacccagc
     4861 gcggggcgat gtcctgggca aagcgggcga ggaagatcca ttgttcgacg gtgaagccgt
     4921 gacggtcggc tcgccggcgc ggcgagaagt cgagtccgtc gctctggaag aggcggccgg
     4981 ggtcggcggc cagtgtgttg cgcgccagtg caacgtgcgt gcgggtttcg ctcggtggtt
     5041 cgtcgtgcgc ggggagcgcg acgcttgcgc cgcgtcgcca ggcgagcagg gtggacagcg
     5101 gccagaagcg cgggccgggc tgcaccttgc ccttgccggc gccgggcggc aggagcagcg
     5161 ggcgcagccc ggtcggcagg tcgcagccgc agccggccgg cagcgcgccg ggctgcagcc
     5221 gctggtaggc gccggcgcca tgcgcgtcgg cgaggagcag cgcgtcggcc ggccggggca
     5281 gcatcggcgt gatctttccg gcgcagcagt gggcgagcag cggcccgtgc gcggcgagcg
     5341 cgcgcagcgc ccgctgttgt tcgcggtcga ccgtgcccgg ccacggcggc ggagcgccgg
     5401 tgtcgccggt gtcgccggtg tcgccggnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5521 nnnnnnncgc ttcgctgcgg tcgtcgagca cttgcgtgcg cagcaggccg gcgagcgacg
     5581 agggccaggg gaaatgcgcg gcgtcgcggc cgccgacgcc gaacggccgg ccggagcgga
     5641 agacgagcgg tgcgcgcggg acgatcaggt agtcgtacat cgtcggcgcg gtggacgccg
     5701 gcggggagtg ttcggcgcgg ggggtcgggt tcatcaatgg ctttccagaa aagcgccgat
     5761 ttcggctgcc gagcgggccg ccaaccagcg cgcgaggatg agctcgtcgc ccagccggtg
     5821 cagggcgccg gggtcgtcgg cggcggcgag cggggcgagc gccgcctgcc gctcggccag
     5881 caggttgcgc gttgcgtggt cgatccgctc gccgccgccg ccctggcggg cgcgcgccag
     5941 cagacggagg aattcgcctt cggcgacgcc ggctccggcg ccgatcgccc ggcagtgcag
     6001 gccaatggcg cgcgtgtcga aggcgaggcg gccgggcagc cggtgcgcgg cgtaggcggc
     6061 ttgccaatga tcgagcgcgg cgcgcccgtg tgcgtcggcg aaagacaggc gcaggccgac
     6121 ttgcggtccg ctgcgcaggc tgagcagcag gccgagcgca ttgccgggcg ccgccggacc
     6181 gccttcgccc ttggccaggc gctcggcgtc ggcggcgcgc tgccggatga cggcgagcgg
     6241 ttcgagcaca tggcagatcg ccgcgccgac gcgcaaggtc ggcggcggtg cgccgtccgc
     6301 cggcggcggc agcgcgccga cgacttcggc cagcgcctcg gtgaaggcgt cgtgcagatc
     6361 gaaggcgccg tggacgacgc cggcgagcgg gaagtagagc gtgaggtcgt cgccgccggc
     6421 aaagacgcat tggccggcga cttcgcgcgc gattcccggc acgcggtcgg caaaggcggc
     6481 gacggcgcgg gaaatccgct catgctcttc tgccgagcgg gcgcggtcga cgaggcgccc
     6541 catgcggtcg ccgtcggcga tgacgagcac cgcataggga cacggctgac cgtggtcgcg
     6601 ccagatcggg cgcagcgtgc ggcgcagttc agcgagcgcg gccagcgcgg ccggctcggc
     6661 gcgccgctcc tgctcggcga cggcgatgcg ttcgggaaag agcaggccgc cgtcgtaggg
     6721 gaagtcggcg tgcgcggtat cgagggcgcc cgtcgcgagg ttgagcgccg ggtcgagcag
     6781 ctttttgtag gcggcgcaga gcgtgctgcg gctcttgtcg tcgagtccgc tcaaccagcc
     6841 atgcgcggca aagcggctga gcgcggtaaa gcgctcgttg cgcccgacaa cgcgtttgat
     6901 gcagccgagc gcgtcgagct gctcgccgcg agcgaggccg aagcgggccg ggaagctctc
     6961 gccggcgggc agttccggca acaccgactc gcgcaggccg tcgaacgagc acttggggat
     7021 gccggcgccc ttgtcggacg ccgccggcgg gaacatcggt gcgaagtcgc gcgtgtgctt
     7081 gcgcgcggcg agcgcgcctt tgagcttgtc gtaggcgatg cggtacggcg ttcgcccgtc
     7141 gcccgacggg tcggcggcct ccgccacgcg cgcccaggcg gcatagctgt cgatcgcatc
     7201 ctcgacctgg cgcgtccaga agtcctggcg cagcctgatg cgtgcgccgt tactgccgcg
     7261 ctgccagtcg tcgagcgcgt tgtcggcgaa gacacgcaga tgagcacgcg ccttgtcctg
     7321 cgctgcgcgc gcgagctcgc gcaccgccgc ctcgtccgcc gcctcgacct tcgccagcag
     7381 gatgttcgac agattgctgt tctcgtccgg cgacgctctg cgcacgcgcg ccgccagcgg
     7441 atagatgagc tgcgcgccgt tgtcgagcag atgccgcgcc gtcgcgccga cgagttcgga
     7501 gagcagatgg ctgcccgccc acaggtcgcg actgcgccgc gcggcggcga tgaagccctg
     7561 caccgggccg atggcgattt cgaggagcca ggtgctcatt
//
LOCUS       DAOQJH010000024         7377 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_5545_length_7377_cov_4.850041, whole genome shotgun sequence.
ACCESSION   DAOQJH010000024 DAOQJH010000000
VERSION     DAOQJH010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 7377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7377
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5545_length_7377_cov_4.850041"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..522)
                     /locus_tag="PLQ18_01115"
     CDS             complement(<1..522)
                     /locus_tag="PLQ18_01115"
                     /inference="COORDINATES: protein motif:HMM:NF022537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2878 domain-containing protein"
                     /protein_id="HNE00276.1"
                     /translation="MSRTGRWLLVNLAAFQIVWWSAVLGAAHGLPWAGPLTALGACAL
                     HVRRIAPETRSAELTMLAYALCFGLACDATLMAFGLIRYAPGLGIGPDWLAPAWMAAL
                     WPTFATTLNHSLGWLRRHPLCAALFGALAGPLAYGGGVVLGAAGWPRGALAGMTAIAA
                     LWAVTLPLLSTLSA"
     gene            complement(519..1781)
                     /locus_tag="PLQ18_01120"
     CDS             complement(519..1781)
                     /locus_tag="PLQ18_01120"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008096313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HNE00277.1"
                     /translation="MNEQTLPAAPAAAAGAGDTADRLLRRVVFQRLRGLARGRLILVD
                     ADGQHSFGPGGHGPQVRVEILDPGVWARIALRGTVGVGEAYVDGGWRADDLTALVRLF
                     VLNKPVMDGMERGLARLALPLLKLWHWLHPNTRHGSRANIAAHYDLGNAFYQTFLDET
                     LMYSCALFEHPGMSLAEASQAKNERICRKLGLRAGDHVLEIGTGWGGFALHAAGRHGC
                     RVTTTTISREQYELARERVAAAGLGNRVTVLFEDYRELAGRYDQLVSIEMIEAVGHRW
                     FDTYFRTCSRLLAPDGQMLLQAITIRDQEYARTKRSVDFIQRHVFPGGCLPSVQALAD
                     TVARVTDLRALHLEDIGPHYATTLRHWRERFLANLDRVRALGFDDTFLRLWEYYLAYC
                     EGGFAERYLGTVQLLLAKPECRRAVLPS"
     gene            complement(1778..2629)
                     /locus_tag="PLQ18_01125"
     CDS             complement(1778..2629)
                     /locus_tag="PLQ18_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008216901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1365 domain-containing protein"
                     /protein_id="HNE00278.1"
                     /translation="MNALPAAAAGLAAPATVPAPGLYVGTVRHRRFAPRAHAFRYPLC
                     MAFVDVDDPEPAFAGRWLWSARRPALAWFRREDHFHLGDPAEPLGDCVRRQVERETGR
                     RPDGAIRLLTQLRWFGHCFNPISLYYCYGAGGGLVALLAEVTNLPWGERHCYVLDLHD
                     QPPAARVPFAKRLHVSPFLPMALDYLWHGYAPGERLGVHLEVFARDRQPGAAGAPLFD
                     ATLHLARRPLDAAQAALALVRYPLMSLQIVAAIHWQALRLWLKRVPVFERPVPPVSTT
                     CPGETDR"
     gene            complement(2626..3897)
                     /locus_tag="PLQ18_01130"
     CDS             complement(2626..3897)
                     /locus_tag="PLQ18_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNE00279.1"
                     /translation="MRYAIVGSGISGLTVAHRLHGRHGQHEFTLYEAGDHVGGHTHTH
                     DVHVGGRGFAVDTGFIVFNERTYPNFIALLDELGVASRPTEMSFSVRCAASGLEYNGH
                     DLNSLFAQRRNLVSPRFWRMLAAILRFNREAPLVLADGTDAHLTLGQYLQRERYPREF
                     IDWYIVPMAAAIWSARPEVMTEIPVLTFVRFFRNHGLLAVRDRPQWRTVCGGSREYVR
                     RLTAPFSGRIRLHTPVTGVRRHAGGVQVLTRAAEPERYDGIIFACHADEALAILGPEA
                     TPAERAVLGAIHYQPNEAVLHTDATLLPHCARARASWNYHLDGQPRTRVPVTYDMARL
                     QGLDCPLPLLVTLNRSEQIDPAKVLRRIGYRHPVFTPEAILAQQRRAEISDRLRSWYC
                     GAYWGYGFHEDGVVSALAVLDELDRHQAVAA"
     gene            complement(3915..4343)
                     /locus_tag="PLQ18_01135"
     CDS             complement(3915..4343)
                     /locus_tag="PLQ18_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013714273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNE00280.1"
                     /translation="MAMLLERFKSLFNELDAGCLDRLAEVYAADVRFRDPVRELHGLE
                     ALRAYYARLYEGVESCRFRYDGEVVEAGGAVVLWTMQFRHRRFHAGETLTLEGASHLE
                     FGQGADARVHRHRDYFDMGEFLYERVPVLGAVIRHIKSGL"
     gene            complement(4765..5079)
                     /locus_tag="PLQ18_01140"
     CDS             complement(4765..5079)
                     /locus_tag="PLQ18_01140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE00281.1"
                     /translation="MNLLTRTAATSLLLALSAGSALADCDGRTGSLMSIPTPVAGSED
                     LPPALAELIAHHRGEIGAPEVSTTQHVQPQTPPAPAVAVLPPNTRVALSGPAATLPST
                     LH"
     gene            5402..6175
                     /locus_tag="PLQ18_01145"
     CDS             5402..6175
                     /locus_tag="PLQ18_01145"
                     /inference="COORDINATES: protein motif:HMM:NF025729.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4412 domain-containing protein"
                     /protein_id="HNE00282.1"
                     /translation="MNARLAAVLIGLCASTGTLPAPADVTLTYALHDDGDGGSQIIAI
                     AGSRLRFETVQEGERAIVLYDGRRHVLTMLDAEQKAYVEFGESSAAELERASAERQRA
                     SDARLGEELAKLPPEERAALEASLRGNVATEAVRVPRRIEQTGQHRSVAGIGCGIVNV
                     WVDDEKQEAHCVASREALGLSEEEFATLTGALSALEQSSGQDGSVAAALGGVPIHSTE
                     RGEEDSELVTELRSVSRAAVPAERLRVPPDYQQQEAPPP"
     gene            6278..>7377
                     /locus_tag="PLQ18_01150"
     CDS             6278..>7377
                     /locus_tag="PLQ18_01150"
                     /inference="COORDINATES: protein motif:HMM:NF024793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNE00283.1"
                     /translation="MYETSFGFTRSPFLDPEGSEFRPCGAQAAVLAELREALDAGAAL
                     VVLSGPIGTGKTTLLRRALGEPMRQRHVVLFWNAHVGPDDLLNYLCDDLDLRSPGTGR
                     TERLKALRTALVRGLSEGWSLLIAVDDAQNLPAETLAELHALLDLREVGHGLVQVLLC
                     GQPELQDRLVHDPQRDAHPPRRLTLRTLDATESAAFIAARLAAAGRPDADLFDAEALV
                     RIHELAHGVPRLLNRICNQSLLLAYLGSERRVSAALVRDVAADTPPTEPPPQPAGPLY
                     EAMPARPVIAADSVPHMHAPVHESVPAAVTGPGNAPAPVQAASAHLHADPHAHGHSHR
                     SSRRRSRWWIGLAAVLMEDGVEADDVIGTLTR"
BASE COUNT         1127 a         2602 c         2681 g          967 t
ORIGIN      
        1 ggccgagagc gtgctcagca gcggcagcgt cacggcccag agcgccgcga tcgccgtcat
       61 tccggccagc gcaccgcgcg gccagccggc ggccccgagc acgacgccgc cgccgtaggc
      121 gagcgggccg gccagcgcgc cgaagagcgc cgcgcacagc ggatggcggc gcagccagcc
      181 gaggctgtgg ttcagcgtcg tcgcgaacgt cggccacagc gccgccatcc acgccggcgc
      241 cagccagtcc ggcccgatgc cgagcccggg cgcgtagcgg atcaggccga aggccatcag
      301 cgtcgcgtcg caggcaagac cgaagcagag cgcgtaggcg agcatcgtca gctccgccga
      361 gcgcgtctcc ggcgcgatgc gccgcacgtg cagggcgcag gctcccagcg ccgtcagcgg
      421 tccggcccag ggcaggccgt gcgcggcgcc gagcacggcc gaccaccaga cgatctggaa
      481 ggcggcgaga ttcacgagca gccagcggcc cgtgcggctc atgacggcag caccgcgcgc
      541 cggcactcgg gcttggccag cagcagctgc acggtgccga gataacgctc ggcgaagccg
      601 ccctcgcagt aggcgaggta gtactcccag aggcgcagga aagtgtcgtc gaagccgagt
      661 gcgcgcacgc ggtcgaggtt ggcgaggaag cgctcgcgcc agtggcgcag cgtcgtcgcg
      721 tagtgcgggc cgatgtcttc gaggtgcagc gcgcgcaggt cggtcacgcg cgcgaccgtg
      781 tcggccagcg cctgcaccga gggcaggcag ccgcccggga agacatggcg ctggatgaag
      841 tcgacgctgc gcttcgtgcg cgcgtactcc tggtcgcgga tcgtgatcgc ctgcagcagc
      901 atctggccgt ccggcgccag cagccggctg caggtgcgga agtaggtgtc gaaccagcgg
      961 tggccgaccg cctcgatcat ctcgatcgac accagctggt cgtagcggcc ggcgagttcg
     1021 cggtaatcct cgaacagcac cgtgacccgg ttgccgagcc cggcggcagc gacgcgctcg
     1081 cgcgcgagtt cgtactgctc gcgcgagatc gtcgtcgtcg tcacgcggca gccgtggcgc
     1141 ccggctgcgt gcagcgcgaa gccgccccag ccagtgccga tctccagcac gtggtcgccg
     1201 gcacgcaggc cgagcttgcg gcagatgcgc tcgttcttcg cctgcgaggc ttcggcgagg
     1261 ctcatgccgg ggtgctcgaa cagcgcgcag gaatacatca gcgtctcgtc gaggaaggtc
     1321 tggtagaagg cgttgccgag gtcgtagtgc gcggcgatgt tggcgcggct gccgtggcgg
     1381 gtgttcggat gcagccagtg ccagagcttc agcagcggca gcgccaggcg cgccagcccc
     1441 cgttccatgc cgtccatgac cggcttgttc agcacgaaca gccgcaccag cgccgtcagg
     1501 tcatcggccc gccagccgcc gtcgacgtag gcctcgccga cgccgaccgt gccgcgcagc
     1561 gcgatgcgcg cccagacgcc ggggtcgagg atctcgacgc gcacctgcgg cccgtgcccg
     1621 ccggggccga agctgtgctg gccgtcggca tcgacgagga tcagccgccc gcgcgcgagc
     1681 ccgcgcaggc gctggaacac cacgcggcgc aacagccggt cggcggtatc gccggcaccg
     1741 gcagcggcgg ccggtgcggc agggagcgtc tgttcgttca tcggtcggtc tccccggggc
     1801 aggtggtgga aacgggcggt acgggccgct cgaacaccgg cacgcgcttg agccacagcc
     1861 gcagcgcctg ccagtggatc gcggcgacga tctgcaggct catcagcggg tagcgcacga
     1921 gcgcgagtgc ggcctgcgcg gcatcgagcg ggcggcgggc gaggtgcagc gtcgcgtcga
     1981 acagcggtgc gccggcggcg ccgggctggc ggtcgcgcgc gaagacttcg aggtgcacgc
     2041 cgaggcgctc gccgggcgcg tagccgtgcc acaggtagtc gagcgccatc ggcaggaacg
     2101 gcgacacgtg caggcgcttg gcgaagggca cgcgcgcggc cggcggctgg tcgtgcaggt
     2161 cgagcacgta gcagtgacgc tcgccccagg gcaggttcgt gacctcggcg agcagcgcga
     2221 cgaggccgcc gccggcgccg tagcagtaat aaaggctgat cgggttgaag cagtggccga
     2281 accagcgcag ctgcgtcagc agccggatcg ccccgtcggg ccgccggccg gtctcgcgct
     2341 cgacctgccg gcgcacgcag tcgccgagcg gctcggccgg gtcgccgagg tggaagtgat
     2401 cctcgcgccg gaaccacgcc agtgccggcc ggcgcgccga ccacagccag cgcccggcaa
     2461 aggccggctc ggggtcatcg acatcgacga aggccatgca gagcgggtaa cggaacgcat
     2521 gcgcgcgcgg cgcgaagcgc cggtggcgga ccgtgccgac gtacagaccc ggagccggca
     2581 ccgtggccgg ggccgcgagg ccggcagccg cagcgggcag cgcgttcatg cggccaccgc
     2641 ctggtggcgg tcgagctcat cgagcacggc cagcgcgctg acgacgccgt cctcgtggaa
     2701 gccgtacccc cagtaggcac cgcagtacca gctgcgcagg cggtcgctga tctcggcgcg
     2761 ccgctgctgc gccagaatcg cctcgggcgt gaacaccgga tggcggtagc cgatgcgcct
     2821 gagcaccttg gccggatcga tctgttcgct gcggttcagc gtcaccagca gcggcagcgg
     2881 acagtcgagg ccctgcagcc gggccatgtc gtaggtcacc ggcacgcgcg tgcgcggctg
     2941 gccgtcaagg tggtagttcc acgaggcgcg cgcgcgtgcg cagtgcggca gcagcgtggc
     3001 gtcggtgtgc agcaccgcct cgttcggctg gtagtggatc gcgccgagca cggcgcgctc
     3061 ggccggcgtc gcctcggggc cgaggatcgc cagcgcctcg tcggcgtggc acgcgaagat
     3121 gatgccgtcg tagcgctcgg gctcggccgc gcgcgtcagc acctgcacgc cgccggcgtg
     3181 gcggcgcacg ccggtcaccg gcgtgtgcag gcggatgcgc ccggaaaacg gcgccgtcag
     3241 ccggcgcacg tactcgcgcg agccgccgca gaccgtgcgc cactgcggcc ggtcgcggac
     3301 tgcgagcagg ccgtggttgc ggaagaagcg cacgaaggtc agcaccggga tctcggtcat
     3361 gacctcgggg cgcgccgacc agatcgccgc cgccatcggc acgatgtacc agtcgatgaa
     3421 ctcgcgcgga tagcgctcgc gctgcaggta ctggccgagc gtcaggtgcg cgtcggtgcc
     3481 gtccgcaagc accagcggtg cctcgcggtt gaagcgcagg atcgccgcga gcatgcgcca
     3541 gaagcgcgga ctgacgaggt tgcgccgctg cgcgaacagg ctgttgaggt cgtggccgtt
     3601 gtattcgagg ccgctcgccg cgcagcgcac gctgaagctc atctcggtcg ggcgcgaggc
     3661 gacgccgagc tcatcgagca gcgcgatgaa gttcggatac gtgcgttcgt tgaagacgat
     3721 gaagccggta tcgaccgcga agccgcgccc gccgacgtgc acgtcgtgcg tgtgcgtgtg
     3781 gccgccgacg tgatcgccgg cttcgtacag cgtgaattcg tgctgtccgt gccgcccgtg
     3841 cagccggtgg gcgacggtca ggccggaaat gccgctgccg acgatcgcgt agcgcatggc
     3901 tcgccctccc ccgctcacag cccggatttg atgtggcgga tcaccgcacc gagcaccggc
     3961 acgcgctcgt acaggaattc gcccatgtcg aagtagtcgc ggtgacggtg cacgcgcgca
     4021 tcggcaccct ggccgaattc gaggtggctc gcgccttcga gcgtcagcgt ctcgccggcg
     4081 tgaaagcggc ggtggcggaa ctgcatcgtc cacagcacga ccgcgccgcc ggcctcgacc
     4141 acttcgccgt cgtagcggaa gcggcacgat tcgacgccct cgtacagccg cgcgtagtag
     4201 gcgcgcagcg cctcgaggcc atgcagctcg cggaccgggt cacggaagcg cacgtcggcg
     4261 gcgtagacct cggccagacg gtcgaggcag ccggcgtcga gttcgttgaa cagcgacttg
     4321 aagcgttcga ggagcatggc catgcgggtc acctgcaaac ctgcaagacg gtgtttcttc
     4381 gccggccggt gtgcggcgct gcctgcgatc gccaccgact tcgacacaac cgacgctgac
     4441 cgtcggaatt ggttcaacaa aacctataca aaaaagccat acaaacaaaa cctctacaaa
     4501 ccgacaggtg ccggctcaca ggcgatgtgc gtgccgccgg cggccgcgga tgcagccggg
     4561 cgaaaaaaac ctgcgccccc gcccccgggc gcctgggcgg acgcccaaaa gaaaccgccg
     4621 tcggcggccg ggcggggaac cggcagacga cggcggcggg tgcgtgcgag ggagaagcgg
     4681 tgcggcaggg aaacggtggc gctgggcgct ctggtgcggg tacggctcag gtccggtgcg
     4741 gggggatggg aaaccggctc gaattcagtg cagcgtggac ggcagcgtgg cggcggggcc
     4801 ggacagggcg acgcgcgtgt tcggcggcag caccgcgacc gccggtgccg gcggtgtctg
     4861 cggctggacg tgctgcgtgg tcgagacttc gggggcgccg atctcgccgc ggtgatgagc
     4921 gatcagctcg gccagcgccg gcggcagatc ctcgctgccc gcgaccgggg tcggaatcga
     4981 catcagcgag ccggtccggc catcgcagtc ggccagcgcg ctgccggcgg acagggcgag
     5041 gaggagggag gtagcagcgg tgcgggtcag aaggttcatc acgatgctcc tgctgtcagg
     5101 ggcgcagcga gtgcgcagta gcgtttgtac agatcatcta caccgacctt cccccgatgc
     5161 ttcgtctgat ttccggcccc tgaagatcag cttttcgggc gatgccaatg tggacaaaac
     5221 ctgtatagcg cagtcacttg ctcaatgaca attttccacg attaaaattt gaacagattc
     5281 tgtactgctt ccgtccccgg tcccggcgat ccgcggccac cgctccgctc ttgggcggcc
     5341 ggtcgatacc ggctactgtg gacgcagctc cttcttcctt cccctcccag gtagtcccgc
     5401 catgaatgca cgcctcgcgg cggtgctgat cggactgtgc gccagcaccg gcacgctgcc
     5461 cgcgcccgcc gacgtcacgc tgacctatgc cctgcacgac gatggggacg gcggctccca
     5521 gatcatcgcg atcgccggca gccggctgcg cttcgagacc gtgcaggaag gggagcgcgc
     5581 gatcgtgctg tacgacggca ggcgccacgt gctgaccatg ctcgatgccg aacagaaggc
     5641 ctacgtcgag ttcggcgaaa gttcggccgc cgaactcgaa cgcgccagcg ccgagcgcca
     5701 gcgcgccagc gatgcccgcc tcggcgagga actcgcgaag ctgccgcccg aggagcgcgc
     5761 cgcgctcgaa gccagcctgc gcggcaacgt cgcgaccgag gccgtgcggg tgccgcggcg
     5821 catcgagcag accggccagc accgcagcgt cgccggcatc ggctgcggca tcgtcaacgt
     5881 ctgggtcgat gacgagaagc aggaagccca ctgcgtcgcc agccgcgagg cgctcggcct
     5941 cagcgaggag gaattcgcga cgctgaccgg cgcattgtcg gcgctggagc agtcatccgg
     6001 tcaggacggc agcgtcgccg ccgccctcgg cggcgtgccg atccattcca ccgagcgcgg
     6061 cgaggaggac tcggaactcg tcaccgaact gcgcagcgtg tcgcgcgccg cggtgccggc
     6121 ggaacgcctg cgcgtgccgc ctgactatca gcagcaggaa gcgccgccgc cctgagccgc
     6181 agcgcgccgc catggatcgt gacgcggcgc atgccccgtc cggcagaccc ggcgctcccc
     6241 gcccgctccc ctgccctgcc gccgacgatt ccgccccatg tacgaaacca gcttcggctt
     6301 cacgcgctcc ccgttcctcg atcccgaggg cagcgaattc cgcccctgcg gggcgcaggc
     6361 cgccgtgctg gccgaactgc gcgaggcgct cgacgccggt gcggccctgg tggtgctcag
     6421 cggcccgatc ggcaccggca agaccacgct gctgcgccgc gcgctcggcg agccgatgcg
     6481 gcagcgccac gtcgtgctgt tctggaacgc gcacgtcggc cccgacgacc tgctgaacta
     6541 cctctgcgac gacctcgacc tgcgctcgcc cggcaccggc cgcaccgagc ggctgaaggc
     6601 gctgcgcacg gcgctggtgc gcgggctgtc cgaaggctgg tcgctgctga tcgccgtcga
     6661 cgacgcccag aacctgccgg ccgagacgct cgccgagctg cacgcgctgc tcgacctgcg
     6721 cgaggtcggc cacggcctgg tgcaggtgct gctgtgcggc cagccggagc tgcaggatcg
     6781 gctcgtgcac gatccgcagc gcgacgccca cccgccgcgg cgcctgacgc tgcgcacgct
     6841 cgatgccacc gagagcgcgg ccttcatcgc cgcacgcctg gccgcggccg gccggcccga
     6901 tgccgatctg ttcgatgccg aagccctcgt gcgcatccat gaactcgcgc acggcgtgcc
     6961 gcggctgctg aaccgcatct gcaaccagtc gctgctgctc gcctacctcg gctccgaacg
     7021 ccgggtctcg gcggcgctgg tgcgcgacgt ggccgccgac acgccgccga ccgagccgcc
     7081 accgcagcct gccggtccgc tgtacgaagc catgccggcg cggccggtga tcgcggccga
     7141 ctcggtgccg cacatgcatg cgcccgtgca cgagagcgtg ccggccgccg tcaccggccc
     7201 gggcaatgcg cccgcgccgg tgcaggcggc gagcgcacac ctgcacgccg atccgcacgc
     7261 ccacggtcac agccaccgct cgtcacgccg gcgcagccgc tggtggatcg ggctggccgc
     7321 ggtgctgatg gaagacggcg tcgaggccga cgacgtgatc ggcactctca cccgcca
//
LOCUS       DAOQJH010000025         7299 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_5628_length_7299_cov_4.909994, whole genome shotgun sequence.
ACCESSION   DAOQJH010000025 DAOQJH010000000
VERSION     DAOQJH010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 7299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7299
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5628_length_7299_cov_4.909994"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            52..948
                     /locus_tag="PLQ18_01155"
     CDS             52..948
                     /locus_tag="PLQ18_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011735873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNE00284.1"
                     /translation="MPAAAVSPYLLLVLTTLFWSGNFVLGRAVHGEIPALALSFWRWA
                     IALLLVLPLAWPQLRAHRGLLLGHWRSLTLLGVLGITNFNSFVYLGLRDTTATNAVLM
                     VSTAPVLIVLLSFLLLGQRPTPRQALGVVVSLSGVAVIVTRGDWHVLAQLALNLGDAW
                     VFAAVVSWALYSVCLRWRPAGLPPLAFLAATMLTGLLALIPIYLWDRLVAGHVLAINS
                     VTLASIAYVAIFPSVLAYIFWNRAVAELGPNRCGQFLHLMPAFGALLSWLLLDERLYA
                     YHWAGIALIATGLWLATAGRRS"
     gene            1777..2517
                     /locus_tag="PLQ18_01160"
     CDS             1777..2517
                     /locus_tag="PLQ18_01160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE00285.1"
                     /translation="MDKNSLLKGLSAALLLTASGSAPAAVSYFNDFSGPSDGKWSNYT
                     TYTTTQATPNSVLGRFAGTGTGTGSTLSLTGLDSHSSVTVEFDLYLFDTWDRHYTGTQ
                     ASPGAVNPYWSPDIVNVTANGTQYASLSYEYASYPYSNTLTGATYTPVSSGQYGGNPQ
                     WNNLATDRIFHITLTFSDLAPNLNLSFATASNSGLSDESYGIDNVRVSTVVSAPSSML
                     LLGLGVVMAARRRYRANSGHSGMQAMAA"
     gene            2832..3137
                     /locus_tag="PLQ18_01165"
     CDS             2832..3137
                     /locus_tag="PLQ18_01165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE00286.1"
                     /translation="MNAQTPAYLQQQKPVDPQLLAAARAALAQLDAALHAARPEAVKA
                     LKDADRRHVVEALNAAAAEVTARLGALPAATRRTLVRDSAAHSPRLAAWFAKHGYDD"
     gene            complement(3171..4931)
                     /locus_tag="PLQ18_01170"
     CDS             complement(3171..4931)
                     /locus_tag="PLQ18_01170"
                     /inference="COORDINATES: protein motif:HMM:NF014927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE00287.1"
                     /translation="MAELPAVQVLSGRLEAGQSVLFALPGLRAGTQLRFRAEGLDGSL
                     DPLLAVLKPGLDLERARIDYRERYQRLRAGRTEDPAAYAALLDEYVLAQADDSAGGYA
                     ADLEFTVPADGDYRLLLSASPLRPGFGGYRLTIAGEEGIAAVSVLAGVWGGDARVQAT
                     GLAFTGDEPWRYVELRPVQAGETLQVRLEAVDGAPLPPQLLLLDYGDKPLATASTSTA
                     APARLTLTHEFRSADPAPRLRVARPDGASGAQYRLLLGIDTAAFGDGPVEPLGRPLLQ
                     VPIEVQVGVRMDQITGVDQRGENFGAVASLRMDWTDPALAFNPESCGCRLKTFGGDAF
                     AQYVQQHGALWPEFVVFNQQGNRWAQKRLVVLRPDGSAQYYERFSTTLQAPDFDFRRF
                     PFDTQRFFIRVELIAPEWKFRFVELPGYSGLGRQLGEEEWVTTGFDTRISSETETTGL
                     PVSRFSYGFTAERHLNYYAYRIFLPVLVIIVIAWVTLFLRDYGKRVEVAGGNLLLFIA
                     FNFTISSDLPRLGYLSFLDTLLLSAFVVTGCMFVLAVYMRRLEAGGASAVLQRIDGWV
                     IWLYPLAYVLGIGAIVAFYR"
     gene            5794..6936
                     /locus_tag="PLQ18_01175"
     CDS             5794..6936
                     /locus_tag="PLQ18_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018609964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HNE00288.1"
                     /translation="MESASARILTGHTQACEVDLAVRELHAQISQPDAALVVVFCSTA
                     YPRPVLAEALQHHFGDVPMIGCTTAGELGASGYQTGGITGFSLPAAEFTVATARLDGL
                     DDVSLDAGQHCVDALLAQMPAVTDGQERFALLLVDGLAQREEPVAYALQRALGRTPLV
                     GGSAGDGLHFGRSWVYHDGAFHTRSAVLALVSTCRRSRVFKTQHFTHDKERLVVTEAD
                     PKRRRVFELNGLPAAQEYARLIGVPVAELAPETFAAAPVVVVIDGTDYVRSIREAHAD
                     GSLTFFCAIELGLVLRVAQARDMVAHLDSSMDSIRAGIGEPDLVISFDCVLRRLEARR
                     KHCEAALGERFRRYRMVGFSTYGEQYAGVHVNHTLTGVAIGRSGDE"
     gene            6929..>7299
                     /locus_tag="PLQ18_01180"
     CDS             6929..>7299
                     /locus_tag="PLQ18_01180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE00289.1"
                     /translation="MNELLPLPDDPEALQAEVLRLRKVVTALIRRAEKSTTSLESAES
                     GFTLYHTAALLEDHVRERTLELEATLAENERVTHDLRLAKQALEAEIAERRRNEAGLR
                     QAAMVYSSTQDGIVVCDPEGTV"
BASE COUNT         1250 a         2569 c         2305 g         1175 t
ORIGIN      
        1 cggcgggcga tgttgtcccc gagtccgtga acctgccgcc ggagcccggc catgcccgcc
       61 gccgccgtgt ccccgtatct gctgctggta ctgaccacgc tgttctggtc cggcaacttc
      121 gtgctcgggc gcgccgtgca cggcgagatt ccggcgctgg cgctgtcgtt ctggcgctgg
      181 gccattgcgc tgctgctggt gctgccgctc gcctggccgc aactgcgtgc ccaccgcggc
      241 ctgctgctcg ggcactggcg ttcgctgacg ctgctcggcg tgctcggcat caccaacttc
      301 aacagcttcg tctacctcgg cctgcgcgac accacggcca ccaatgccgt gctgatggtg
      361 tcgacagcgc cggtgctgat cgtgctgctg tccttcctgc tgctcggcca gcgcccgacg
      421 ccgcgccagg cgctcggcgt ggtcgtgtcg ctgtcggggg ttgccgtcat cgtcacccgc
      481 ggcgactggc acgtgctcgc gcaactggcg ctgaacttgg gcgatgcctg ggtgttcgcg
      541 gccgtggtca gctgggcgct gtattcggtc tgcctgcgct ggcggccggc cggcctgccg
      601 ccgctcgcgt tcctggccgc gacgatgctg accggactgc tggcgctgat cccgatctac
      661 ctgtgggacc ggctggttgc cgggcatgta ctggcaatca attccgtgac gctcgcgagc
      721 atcgcctacg tcgcgatctt cccgtcggtg ctcgcctaca tcttctggaa ccgcgccgtc
      781 gccgaactcg gcccgaaccg ctgcggccag ttcctgcacc tgatgccggc cttcggcgcg
      841 ctgctgtcct ggctgctgct cgatgagcgc ctgtacgcct accactgggc gggcatcgca
      901 ctgatcgcca ccggcctgtg gctggcgacc gccggccggc ggtcctgaaa ccggaaccgc
      961 gctgcctgaa gcgaacgaac ccccatcggc acaatggttc acattcactg aaccagacca
     1021 gggcgccaca accgtgatgc ctccggcgcg gccgggccac acaggacgat ccggcccgtg
     1081 ctgcctcacc tgcacacatc cagcgagacc ggcctgcatc cggcgccctg gcccgatcac
     1141 cggcgaccgc tcccaggcat gggcgcaagt ccctgacagc cgccgttcga atcatccacc
     1201 ggtcgcgata cgtgcctgat tcatcagaca cgttttgccg gacgcatccg gatgccggcg
     1261 agccggggac ccgccgcgca gcggacgtat catctttcat ccggttcatg aatatttcgt
     1321 tattcaggaa ccatttcgaa atgcagtgct gcgcagccga attcagggct gcaacgacgg
     1381 aaatccggaa acccgcagcc ccggtcgcca tgatccatgc cgctttgccc ccggccaccg
     1441 agcgcgcggc aggcggcgca gacgggatgc ccggccggca tgaggcctgc ggcccgtgcg
     1501 ttccggctgt tgccatcctt tacgtgaccg accccagacg ctaacctcgc gccagtcaaa
     1561 aactggatgc tttgcccgcg ctgcggatcg agcgcccccg tcggagccaa ccgacgcagc
     1621 cacgatcctg caagccttct gccccactgc cgaacagcag cgcctggtgg ggatggtccg
     1681 agcgggcacg ctgcctggag cagcgtgcga gattcaggta ccgccctggt acggcagggg
     1741 gtagcgcgct gccgaaagca tcatcggaag ggaaacatgg acaagaattc actgctcaag
     1801 ggcctttcgg ccgcactcct gctcacagct tccggatcgg ctcctgccgc cgtttcctat
     1861 ttcaatgact tctcgggtcc cagcgacggc aagtggtcga attacacgac atacacgacg
     1921 acccaggcca ccccgaattc cgttcttgga cgatttgccg gcacgggcac cggtactggc
     1981 tccacgctgt cgctgaccgg tctcgacagc cattcctccg tgacggtcga atttgatctc
     2041 tatctgttcg acacctggga tcgacactac accggcaccc aggccagccc gggcgcggtg
     2101 aatccttact ggtcgccgga cattgtcaac gtcacggcca atggcaccca gtacgcttcc
     2161 ctcagttacg aatacgccag ctacccgtac agcaacaccc tgacaggagc cacgtacact
     2221 ccggtcagct ccggccagta tggtggcaac cctcaatgga acaatcttgc caccgacagg
     2281 atcttccaca tcacgctgac cttttccgat ctcgcaccga atctcaatct gagcttcgca
     2341 acggcgagta acagcggctt gtccgacgaa tcctacggta tcgacaacgt ccgcgtttcc
     2401 acggtcgttt ctgccccttc gtcgatgctg ctgctcggtc tcggtgtcgt catggccgcc
     2461 cgtcgccgtt atcgggcgaa ctccggtcat tccggcatgc aggcaatggc tgcctgagac
     2521 ccggcactgc acgcggcagg gcaaaactgc aggatcggtg atgccacccg tacctttccg
     2581 ggtggcggcg catctgtcag caccttcgcc cgccccctgc ccggattgat cattcctgca
     2641 tcctgcctga atgctataac tcggccgttt ccgggtttcg gccggtcacc ccaccgtccc
     2701 gggtgctccc gccgccggat gatccgggac gtggtcatgc caatgcggag gtcatcgcca
     2761 cgatgccggt cccgaccttc caggtgcccc accccgcccc cggccgtggg gcacaggccg
     2821 ccgcgcaagg catgaacgcg cagacgccgg cctatctgca gcagcagaaa ccggtcgatc
     2881 cgcaactgct cgccgccgcc cgcgccgcgc tcgcgcaact cgatgccgcc ctgcacgccg
     2941 cacgtccgga agccgtgaag gccctgaagg acgcggatcg ccgccatgtc gtcgaagccc
     3001 tgaacgccgc cgccgccgaa gtcaccgccc gcctcggcgc gcttccggcc gccacgcgca
     3061 ggacgctggt ccgcgacagc gccgcacaca gcccgcggct tgcggcctgg ttcgcgaagc
     3121 acggctacga cgactagccg accggcggca ccgccgccgc cgcatccggc tcagcggtag
     3181 aacgccacga tcgcgccgat gccgagcacg tatgccagcg gatacagcca gatcacccag
     3241 ccgtcgatac gctgcagcac agcgctggcc ccgccggcct ccagcctgcg catgtacacg
     3301 gcgagcacga acatgcagcc ggtgacgacg aaggcactaa gcagcagcgt atcgaggaag
     3361 ctcaggtagc cgagccgtgg caggtcgctg gagatcgtga agttgaaggc gatgaacaac
     3421 agcaggttgc cgccggcgac ctcgacgcgc ttgccgtagt cacgcaggaa cagcgtcacc
     3481 caggcgatga cgatgatcac cagcaccggc aggaaaatcc ggtacgcgta gtaattcagg
     3541 tgccgctcgg ccgtgaagcc gtaggaaaac cgcgataccg gcaggccggt ggtttcggtc
     3601 tcgctgctga tgcgggtgtc gaaaccggtc gtgacccatt cctcctcgcc aagctggcgg
     3661 ccgagaccgc tgtagccggg cagttcgacg aaacggaatt tccactccgg cgcgatcagt
     3721 tcgacgcgga tgaaaaaccg ttgcgtatcg aacggaaaac gccggaaatc gaaatccggt
     3781 gcctgcagcg tcgtcgagaa gcgctcgtaa tactgcgcgg aaccgtccgg gcgcagcacg
     3841 acgaggcgct tctgcgccca gcggttgccc tgctggttga acacgacgaa ctccggccac
     3901 agcgcgccgt gctgctgcac gtactgcgcg aaggcgtcgc cgccgaaggt cttcagacgg
     3961 cagccgcagg actcgggatt gaaggccagt gccgggtcgg tccagtccat gcgcagcgag
     4021 gcgacggcac cgaagttctc gccacgctgg tcgacgccgg tgatctggtc catgcgcaca
     4081 ccgacctgca cctcgatcgg cacctgcagc agcggccggc cgagcggctc gaccggaccg
     4141 tcgccgaagg cggcggtgtc gatgccgagc agcagccggt actgcgcgcc gctcgcgcca
     4201 tcgggacgcg cgacgcgcag gcgcggtgcc gggtcggcgc tgcgaaactc atgcgtcagc
     4261 gtcagtcgcg ccggcgcagc ggtgctggtg ctggcggtgg cgagcggctt gtcgccgtag
     4321 tcgagcagca gcagttgcgg tggcagcggc gccccgtcga cggcttcgag ccgcacctgc
     4381 agcgtctcgc cggcctgcac cggccgcagc tcgacatagc gccagggttc gtcgccggtg
     4441 aaggcgagtc cggtcgcctg cacgcgcgca tcgccgcccc agacaccggc caggacgctg
     4501 accgccgcga ttccctcctc gccggcgatc gtcagccggt agccgccgaa gcccggacgc
     4561 agcggacttg cgctcagcag cagccggtag tcgccgtcgg ccggcaccgt gaactccaga
     4621 tcggccgcat aaccgccggc gctgtcgtcg gcctgcgcga gcacgtactc gtcgagcagc
     4681 gccgcgtagg ctgccgggtc ctcggtgcgc ccggcacgca agcgctggta gcgctcgcgg
     4741 tagtcgatgc gcgcccgctc gagatcgagc cccggcttca gcaccgcgag cagcggatcg
     4801 agactgccgt cgaggccctc ggcccgaaac ctcagctgcg tgccggccct cagtcccggc
     4861 agtgcaaaca gcacgctctg cccggcttcg agccggccgc tcaggacctg caccgcagga
     4921 agttcagcca cggccccggg gggccacgag gcgagccctg cacatacaca cagccagcac
     4981 aagacgatga gacggcgaca gccagggagc ggagacattc ggggtgcagg tccggggagg
     5041 attcgatccg tcacagcgta gcggtacaga actgcaacga ctgacgcacg atgtgttccg
     5101 gcatgggcgc aggagcgttg caaaccgcga attgcccccg gcacgcaccg cttcgcctgc
     5161 gctaattgac gcaccgcaaa aatgtggccg ctggtgaaac tgcctgctga aacgagagca
     5221 aacgcttaat tccattgaac ctgggccgat atgcaaggct tgcaatgatt gcgcaggcag
     5281 accggctcaa ccgtgcttga cggcaatggc atatggcttg cctacggagg aattccgcgc
     5341 ttcaacggcc gcgaccacca ccgccgacac ctacgccgac aaccgtgccg tcacgaagac
     5401 ttgtcgattt tgcaccgcac aatccagacc atgaacgccg ctgcaaaggc catgaccaca
     5461 agcttctgtc cattattgcc gtgctcagtg catactgttc aagcagttgc ctcagtacgt
     5521 cctgcaggaa tctgtatcgg tacagacctc gcgacgaggc cctgcaggcc cgcttctgac
     5581 atggacaggc ggatgggctg ctgcacccgg aacgcttcag aaaccgaaat gatgcaaatg
     5641 caatagcaat caatgtcgct acggcaattc cgacattcct tccggaaaac cccaatggcc
     5701 tgtaatgccg cccgccggag cagcccggaa tccggtgcaa accagaaagc gcaggaagga
     5761 ccccgccatg tgaataccgc cgaggagtca tcgatggaat ccgcttccgc ccgcatcctg
     5821 accggccaca cgcaggcatg cgaggtcgac ctggccgtgc gcgaactgca tgcccagatc
     5881 agtcagcccg atgcggcgct ggtcgtggtg ttctgttcga ccgcctatcc gcgtccggtg
     5941 ctggccgaag ccctgcagca tcatttcggt gacgtgccga tgatcggctg cacgaccgcc
     6001 ggggaactcg gcgccagcgg ctaccagacc ggcggcatca ccggcttcag cctgccggcg
     6061 gcagaattca cggtggcgac cgcccggctc gacggtctgg acgacgtcag cctcgatgcc
     6121 ggccagcact gtgtcgatgc cctgctcgcg cagatgccgg cggtgacgga cggacaggag
     6181 cgcttcgccc tgctgctggt cgatggcctc gcgcagcgcg aggaaccggt cgcctatgcg
     6241 ctgcagcgcg cactcgggcg cacaccgctg gtcggcggct cggccggtga cggtctgcac
     6301 ttcggccgca gctgggtcta tcacgatggc gccttccaca cccgcagcgc cgtgctggca
     6361 ctggtcagca cgtgccggcg cagccgggtg ttcaagaccc agcacttcac ccacgacaag
     6421 gaacgcctgg tggtcaccga ggccgatccg aagcgccggc gggtgttcga gctgaacggc
     6481 ctgccggcgg cgcaggaata tgcccgcctg atcggcgtgc cggtggcgga gcttgccccc
     6541 gagaccttcg cggccgcacc ggtggtcgtg gtcatcgacg gcaccgatta cgtgcgctcg
     6601 atccgcgagg cccacgccga cggcagcctg accttcttct gtgcgatcga actcgggctg
     6661 gtgctgcgcg tcgcgcaggc ccgcgacatg gtcgctcacc tcgactcgtc gatggacagc
     6721 atccgcgccg ggatcggcga accggatctg gtcatcagct tcgattgcgt gctgcgccgg
     6781 ctcgaagccc ggcgcaagca ctgcgaggct gcgctcggcg aacgcttccg gcgctatcgc
     6841 atggtcgggt tcagcaccta cggcgagcag tacgccggcg tgcatgtcaa tcacacgctg
     6901 accggcgtcg cgatcggcag gtccggtgat gaatgaactc ctgcccctgc cggacgaccc
     6961 cgaggccctg caggcggaag tcctgcgact gcgcaaggtg gtgacggcgc tgatccggcg
     7021 cgccgagaaa tcgacgacca gcctggagag cgccgagtcc ggcttcacgc tgtatcacac
     7081 ggccgcgctg ctcgaagacc acgtgcgcga gcgcacgctc gagctcgagg cgacgctggc
     7141 cgagaacgag cgcgtcacgc acgacctgcg cctcgccaag caggcgctgg aggccgagat
     7201 tgccgagcgc cggcgcaacg aggccggcct gcgccaggcg gcgatggtct attcgagcac
     7261 gcaggacggc atcgtcgtct gcgaccccga gggcacggt
//
LOCUS       DAOQJH010000026         7261 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_5667_length_7261_cov_4.210103, whole genome shotgun sequence.
ACCESSION   DAOQJH010000026 DAOQJH010000000
VERSION     DAOQJH010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 7261)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7261)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7261
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5667_length_7261_cov_4.210103"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..410)
                     /locus_tag="PLQ18_01185"
     CDS             complement(<1..410)
                     /locus_tag="PLQ18_01185"
                     /inference="COORDINATES: protein motif:HMM:NF024809.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HNE00290.1"
                     /translation="MSRKDSDLTTTQGDRPMSQITLVTGTRNNSPWSLRPWLLMRQLG
                     IPFDEIRLPIGARERLPDGGRWSPESGIPVLKHGALTVRDSLAIAEYLNEALAPGRVW
                     PADLASRAVARSVSAEIHSNFSVFRRELPMDTIG"
     gene            complement(723..2048)
                     /locus_tag="PLQ18_01190"
     CDS             complement(723..2048)
                     /locus_tag="PLQ18_01190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE00291.1"
                     /translation="MSALPVTELRRARPAAGLLASMLVLTLASAFVPALTPWPAAACA
                     WSAAALLWPRLPQRQRRQALGMAGIGVAGMLAGVLSGVPADLRLAATGNQTLIGLLCA
                     VSFLRLVTMPDAQTRSEALPRGRTALWRTLLGVHLFGAVINIPAVFILADRLLRNGRL
                     GRRAALVLTRGFCACTFWSPFFAAMAAALNFAPGARLGIIILTGLPLAALALWLTALD
                     TDDGRRNTRGAGFVGYPMHLRTLAVPGALVGLVLGTHLLWPQTGITTIVTLYAPLLCL
                     VVLLGRRGRAGLHAFAGHALRRLGDGAPELVLFLAAGLLAAGITGLLAATGGQLPFVR
                     FGPIEASLTLLLIVAAAFAGVHPVIAITTFGTWLAPLAPDPTLLTSTLTMGWAIGVPA
                     GPLSGLHLSMQGRYGIPVFAFQRWNSRYIPKLLAASILALHLQAALRGL"
     gene            2186..2893
                     /locus_tag="PLQ18_01195"
     CDS             2186..2893
                     /locus_tag="PLQ18_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007191214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNE00292.1"
                     /translation="MPYIPTQNAWNGEADCRTCSVRGSVLFAGLQQNDFDLIHQPIDD
                     IAVPAQGRVYHVHEPGARVFTLRSGLVKLVQYLPDGSQRIVRLLRGNDVLGLEALLGQ
                     PYQHDAIALTAVELCAIPREVVAVLAREQPALHTELLKRWQQALSDADVWLTELSTGT
                     ARARVARLLLRLAGPRPVEAFRLLGREDIGAMLGLTPETVSRTVAELRRQKLLREQGA
                     GLFTVDAPALRRIADGG"
     gene            2945..3652
                     /locus_tag="PLQ18_01200"
     CDS             2945..3652
                     /locus_tag="PLQ18_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008060297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HNE00293.1"
                     /translation="MTGIRALLFDVFGTLVDWRTSVAREAEAVLAPLGHALDWPAFAD
                     DWRARYQPAMEAVRCGLEPYVALDELHRRNLDAVLASRGIELDEARCRHLNLAWHRLD
                     AWPDVGDGLAALRAPFLLAPCSNGNIALMVALARRNGWHWDAILGAEPARAYKPQPLV
                     YLHAVAALGLTPAETLMVAAHAADLAAAGALGLRTAFLARAAEYSRPNPADQPDFPVD
                     FHVDSLPALAAQLRVGG"
     gene            complement(3657..6863)
                     /locus_tag="PLQ18_01205"
     CDS             complement(3657..6863)
                     /locus_tag="PLQ18_01205"
                     /inference="COORDINATES: protein motif:HMM:NF019874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNE00294.1"
                     /translation="MPGTPLRRLTDEDYLPWLQVGVSFVLIAALVLALGWWFIADYRR
                     EVERRIDQLERETLAQQETLLRRRVDETRIWLDWLRSRAETLLAERVREQAEAAYELV
                     YSMHRLLDGPLGTAETQKLIVETLRPLRFFDGRGYYFIDTLDGDCVLLPTSPEREGHS
                     LLPIRDDNGVCIMCELIRVATQPEGAGLLRYRWYPPNDATRMDDKLTWVRRFEPYRWL
                     LGTGEYLDTMMRMLQREALDRLRALRFEDDGYIAVFHRDGRMMVTPANPDLEGSNGRT
                     TLDATRRAVLEQLLASAGAGGGVVRYDWNRPAGGPLRTKLSVVTPFDAWGWTLVAGLY
                     LDDLDVTLASQRGELEAGVRARERATLWALALALSVGLGSSIAYARWVQARLRARRRE
                     LEASARRLYLTQRCVDAAADSIFLADAGLCVVYANDAACRTLGRPAAAITGRSLGELG
                     LAPPGVPTQPDSYEATPTGPDGRSLALEVAWTPLETGGERYACAIARDVAERKRVEAE
                     LRLASLVFEIGNDAMMITDADNRILAVNPAFTRVTGYAPAEAIGCNPNLLSSGRQPEA
                     FYAALWDALARKGHWSGELWNRRKDGSAYPQWLSISVLRDAQGRAVRHVAAFTDISER
                     KANEARIRHLADYDPLTDLPNRTLLRERIQQTMHAAASGGPGFALVFLDLDHFKHIND
                     TLGHAAGDALLCAVGTRLRSVVRSGDTVSRTGGDEFVLLLPGLVEAADTERLTAKLMR
                     TLAEPYLIGGKELRVTPSLGYACYPHDATDIDTLLGHADAAMYQAKAQGRNNAQPFTP
                     RLSAHLAERFELENALRGALERQELELHYQPQYFVDGRPRGAEALLRWQHPQLGRVSP
                     ERFIPIAEETGLIRPIGDWVLRSACAQAVAWQRAGIPPLTIAVNVSAVQFAQADWVDT
                     VAATLGASGLAPCCLQLELTESVLMKDPAAAALTLKALRATGVRLSIDDFGTGYSSLA
                     YLKRFPLDELKIDRSFIDGLPGDAEDAAIVHAILQLARSLGLETVAEGVETEAQRRFL
                     QAHGCTAVQGWLYAPALPAGAAGERLYPPACRASGRAN"
BASE COUNT         1072 a         2496 c         2665 g         1028 t
ORIGIN      
        1 tcgccgatcg tatccatcgg cagctcgcgc cggaacaccg agaaattgct gtggatctcg
       61 gcggacaccg agcgggcgac ggcacggctg gcgagatcgg cgggccacac gcggccgggg
      121 gcgagggctt cgttcaggta ttcggcgatc gccagcgaat cgcgcacggt cagcgcgccg
      181 tgtttcagga cggggatgcc ggactccgga ctccagcgac cgccatccgg gagccgctcg
      241 cgcgcaccga tcggcaggcg gatctcatcg aaggggatgc cgagctggcg catcagcagc
      301 cagggacgca gcgaccacgg ggagttgttc cgggtgccgg tgacgagcgt gatctgactc
      361 atggggcggt ctccctgggt tgttgttaga tcgctgtcct tgcgcgacac ggctgtatcc
      421 ggatgcagac gtatcgggat gattcaggaa tttccggacc ggcgcaaacc gtcagcgagt
      481 gtggaatttt aaggtgtgcg ccagcgcccc gttgccgccg ccacccctgc tgaccacctc
      541 gtcggcccgg atcgggcatt cctgccgccg ggccagcgac cagtccggcg gtcggttgat
      601 aacgtgctga atgttacagg atttgtcaaa gtatttgtca taacgagttt gtaaggtccg
      661 ggcgtgcttg acaagcgcgg ctcgcacccg gtttgtaacc gtccgccctc ccgcggcagc
      721 cgtcacagac cgcgcagggc ggcctgcagg tgcagcgcga ggatcgatgc ggccagcagc
      781 ttcgggatgt aacggctgtt ccagcgctgg aacgcaaaca ccgggatgcc gtagcgcccc
      841 tgcatcgaca ggtgcagccc cgacagcggt ccggccggca cgccgatcgc ccagcccatc
      901 gtcagcgtgc tggtcagcag cgtcgggtcc ggcgcgagcg gcgcgagcca ggtgccgaag
      961 gtcgtgatcg cgatcaccgg atgcacgccg gcgaaggccg ccgcgacgat cagcagcagc
     1021 gtcaggcttg cctcgatcgg cccgaagcgc acgaacggca gctgcccgcc ggtggcggcg
     1081 agcagcccgg tgatgccggc ggcgagcagg ccggcggcga ggaacagcac cagttccggc
     1141 gcgccgtcgc cgaggcggcg cagcgcgtgc ccggcgaaag cgtgcagccc cgcgcggccg
     1201 cggcgcccga gcagcacgac gaggcagagc agcggcgcat acagcgtgac gatggtcgtg
     1261 atgccggtct gcggccacag caggtgcgtc ccgagcacca gcccgaccag cgcccccggc
     1321 accgccagcg tgcgcaggtg catcggatag ccgacgaagc ccgcaccgcg cgtgttgcgg
     1381 cggccgtcgt cggtgtcgag tgccgtcagc cacagcgcca gggctgccag tggcaggccg
     1441 gtgaggatga tgatgccgag ccgcgccccg ggcgcgaagt tcagcgccgc cgccatcgcg
     1501 gcgaagaacg gtgaccagaa cgtgcaggcg cagaagccgc gcgtcagcac cagcgccgcg
     1561 cgccggccga ggcggccgtt gcgcagcagc cggtcggcga ggatgaacac cgccgggatg
     1621 ttgatgacgg cgccgaacag atgcacgccg agcagcgtgc gccagagcgc cgtgcgcccg
     1681 cgcggcagcg cttcgctgcg ggtctgtgcg tccggcatcg tcaccagccg caggaaggac
     1741 acggcgcaga gcagtccgat cagcgtctgg ttgccggtgg cggcgaggcg cagatccgca
     1801 ggcacgccgg acagcacgcc tgccagcatg ccggcaacgc cgatgccggc catgccaagc
     1861 gcctgccggc gctggcgctg cggcaggcgc ggccacagca gggcggcggc gctccaggcg
     1921 caggcggcgg ccggccacgg cgtcagcgcc ggcacgaagg ccgaggcgag cgtcagcacg
     1981 agcatcgagg caagcagccc ggcggccggc cgggcgcggc gcagttcggt cacgggcagg
     2041 gcgctcatgc gaggaggcgg ggcgggcggg gactgcaggg ttcgcagccg ccggcgctcg
     2101 cgtgccggcg gctgcgctca caggcattcc gcaggggcga agacttgtat ataaaccgcc
     2161 ggcccggcgg tttatataca agtccatgcc ctacatcccg acccagaacg cctggaacgg
     2221 cgaagccgac tgccgcacct gcagcgtgcg gggctcggtg ctgttcgccg gcctgcagca
     2281 gaacgatttc gatctgatcc accagccgat cgacgacatc gccgtgccgg cgcagggccg
     2341 ggtctatcac gtccatgaac cgggcgcgcg cgtgttcacg ctgcgcagcg gcctggtcaa
     2401 gctcgtgcag tacctgcccg acggcagcca gcgcatcgtg cggctgctgc gcggcaacga
     2461 cgtgctcggg ctcgaagccc tgctcggcca gccctaccag cacgatgcga tcgcgctgac
     2521 cgcggtcgag ctctgcgcga ttccgcgcga ggtggtcgcg gtgctggcgc gcgagcagcc
     2581 ggcgctgcac accgagctgc tgaagcgctg gcagcaggcg ctcagcgatg ccgatgtctg
     2641 gctgacggag ctttcgaccg gcacggcgcg cgcgcgcgtc gcgcgactgc tgctgcgtct
     2701 ggccggcccg cggccggtcg aggcgttccg gctgctcgga cgcgaggaca tcggcgcgat
     2761 gctcggcctg acgcccgaga ccgtcagccg caccgtcgcc gaactgcgtc gccagaagct
     2821 gctgcgcgag cagggcgccg gcctgttcac ggtcgatgcg ccggcgctgc gccggatcgc
     2881 cgatggcggc tgatgcgcgc tgccgccgtt cccgtcccgt cgtctgctgt cgtgaggtgg
     2941 agcaatgacc ggcatccgcg ccctgctgtt cgacgtgttc ggcacgctgg tggactggcg
     3001 cacttcggtg gcccgcgaag ccgaagccgt gctggcgccg ctcgggcatg ccctcgactg
     3061 gccggcgttc gccgatgact ggcgcgcgcg ctaccagccg gccatggagg cggtgcgctg
     3121 cggtctggag ccctatgttg cgctcgatga actgcaccgg cgcaacctcg acgccgtgct
     3181 cgcgagccgc ggcatcgagc tcgatgaagc ccggtgccgg cacctgaacc tcgcctggca
     3241 ccgcctcgac gcctggccgg atgtcggcga cgggctcgcg gccctgcgcg cgccgttcct
     3301 gctggcaccg tgctcgaacg gcaacatcgc gctgatggtg gcgctggcgc gccgcaacgg
     3361 ctggcactgg gatgcgatcc tcggggccga gccggcgcgg gcctacaaac cgcagccgct
     3421 cgtctacctg cacgcggtcg ccgcgctcgg cctcacgccg gccgaaaccc tgatggtcgc
     3481 ggcgcatgct gccgatctcg ccgccgccgg cgcgctcggc ctgcgcacgg ccttcctcgc
     3541 gcgtgccgcc gaatacagcc ggccgaaccc cgccgaccag ccggatttcc cggtcgattt
     3601 ccacgtcgac agcctgccgg ccctggcggc acaactgcgg gtcggcggct gagaagctaa
     3661 ttcgccctgc ccgatgcccg gcaggccggc gggtacagcc gttcgccagc cgccccggcc
     3721 ggcagggccg gggcgtacag ccagccctgg accgccgtgc agccgtgcgc ctgcaggaag
     3781 cggcgctggg cttcggtttc gacgccttca gcgaccgtct ccaggccgag gctgcgcgcg
     3841 agctgcagga tggcgtgcac gattgcggcg tcctcggcgt ccccgggcag gccgtcgatg
     3901 aagctgcggt cgatcttcag ctcgtcgagc gggaagcgct tcaggtacgc gaggctcgaa
     3961 tagccggtgc cgaagtcgtc gatcgacaga cgcacgccgg tcgcgcgcag cgccttcagc
     4021 gtcagcgcgg ctgcggccgg gtccttcatc agcacgctct cggtcagttc gagctgcagg
     4081 cagcagggcg cgaggccgga agcgccgagc gtggcggcga ccgtgtcgac ccagtcggcc
     4141 tgcgcgaact gcacggcgga aacattgacg gcgatggtca gtggcgggat gcctgcccgt
     4201 tgccaggcca cggcctgcgc gcaggcgctc cgcagcaccc agtcgccgat cggccggatc
     4261 agtccggttt cctcggcgat ggggatgaag cgctcgggcg agacgcgccc gagctgcgga
     4321 tgctgccagc gcagcagcgc ctcggccccg cgcgggcggc cgtcgacgaa gtactgcggc
     4381 tggtagtgca gttccagttc ctggcgttcg agcgccccgc gcagcgcgtt ctccagctcg
     4441 aagcgctcgg ccagatgcgc gctcaggcgc ggcgtgaacg gctgggcgtt gttgcggccc
     4501 tgcgccttgg cctggtacat ggccgcgtcg gcgtggccga gcagcgtgtc gatgtcggtg
     4561 gcatcgtgcg ggtagcaggc gtagccgagg ctcggcgtca cgcgcagctc cttgccgccg
     4621 atcaggtacg gctcggccag cgtgcgcatc agcttggcgg tgagccgctc ggtgtcggcc
     4681 gcctcgacca gaccgggcag cagcagcacg aactcgtcgc cgccggtacg gctgaccgtg
     4741 tcgccgctgc gcacgaccga gcgcagccgc gtgccgaccg cgcagagcag cgcgtcgccg
     4801 gcagcgtggc cgagcgtgtc gttgatgtgc ttgaaatgat cgaggtcgag aaacaccagc
     4861 gcgaagcccg gtccgccgct cgcggcggcg tgcatcgtct gctggatgcg ctcgcgcagc
     4921 agcgtgcggt tcggcaggtc ggtcagcggg tcgtagtcgg cgaggtggcg gatgcgggcc
     4981 tcgttcgcct tgcgttcgct gatgtcggtg aacgcggcga catggcgcac ggcgcgacct
     5041 tgcgcgtcgc gcagcaccga gatgctcagc cactgcggat aggccgagcc gtccttgcgc
     5101 cggttccaga gctcgccgct ccagtgaccc ttgcgcgcga gcgcgtccca gagcgcggca
     5161 tagaacgcct ccggctggcg gcccgaggac agcaggttcg ggttgcagcc gatggcctcg
     5221 gccggcgcat agccggtcac gcgcgtgaag gccgggttga cggcgaggat gcggttgtcg
     5281 gcatcggtga tcatcatcgc gtcgttgccg atctcgaaca ccaggctcgc gaggcgcagc
     5341 tcggcttcga cgcgcttgcg ttcggccaca tcgcgcgcga tcgcgcaggc atagcgctcg
     5401 ccgccggttt cgagcggcgt ccacgccacc tccagcgcca gcgaccgccc gtcggggccg
     5461 gtcggggtcg cctcgtagct gtccggctgc gtcggcacgc cgggcggtgc gagaccaagc
     5521 tcgccgaggc tgcgaccggt gatggccgcg gccggacgcc cgagcgtgcg gcaggcggcg
     5581 tcgttggcat agacgacgca aagccccgca tcggcgagga agatcgaatc ggcggccgcg
     5641 tccacgcagc gctgcgtcag gtacagccgg cgtgcgctcg cttcgagctc ccggcgccgg
     5701 gcgcgcagcc gcgcctgtac ccagcgcgcg taggcgatcg agctgccgag gccgacactc
     5761 agcgccagcg ccagcgccca cagcgtggcg cgctcgcgcg cccggacgcc ggcttcaagc
     5821 tcgccgcgct ggctggcgag cgtgacatcg aggtcatcga gatacagacc ggcgaccagc
     5881 gtccagcccc aggcgtcgaa gggcgtgacc accgagagct tcgtgcgcag cggaccgcct
     5941 gcgggccggt tccagtcgta gcgcacgacg ccgccgccgg caccggcgct cgcgagcagc
     6001 tgttcgagca ccgcacggcg cgttgcatcg agggtggtgc ggccgttgct gccttcgaga
     6061 tcgggattcg ccggcgtgac catcatccgg ccgtcgcggt ggaacacggc gatgtagccg
     6121 tcgtcctcga agcgcagcgc gcgcagccgg tcgagtgcct cgcgctgcag catgcgcatc
     6181 atcgtgtcga ggtattcgcc ggtgccgagc agccagcgat acggctcgaa gcgccgcacc
     6241 caggtcagct tgtcgtccat gcgcgtggcg tcgttcgggg gataccagcg atagcgcagc
     6301 aggccggcac cttcgggctg cgtcgcgacg cggatcagct cgcacatgat gcagacgccg
     6361 ttgtcgtcgc ggatcggcag cagcgagtgg ccctcgcgct ccggcgaggt cggcagcagc
     6421 acgcagtcgc catcgagcgt atcgatgaag tagtagccgc gaccgtcgaa gaagcgcagc
     6481 gggcgcagcg tctcgacgat cagcttctgc gtttcggccg tgccgagcgg gccgtcgagg
     6541 aggcggtgca tcgaatagac cagttcgtac gcggcctcgg cctgctcgcg cacgcgctcg
     6601 gcgagcagcg tctcggcacg cgagcgcagc cagtcgagcc agatccgggt ttcgtcgaca
     6661 cggcggcgca gcagggtttc ctgctgtgcg agcgtttcac gttcgagctg atcgatccgg
     6721 cgttcgacct cgcggcggta gtcggcgatg aaccaccagc cgagcgcgag cacgagcgcg
     6781 gcgatcagta caaaggacac accgacctgc agccagggca gatagtcctc atcggtgagc
     6841 cggcgcaggg gggtaccggg cattgcgggt ttcatggtgc gaaattttcc accatgccat
     6901 cgacctgacg catgggcggg ctcgcaggga cacacaactt atctgtttgt taatgttctg
     6961 gccattccct ttgcaccggc cgctccggcg gggctgtgcc ggcagggaag gcggcggaag
     7021 tccggagtgt ccggaacaag cgcacgggaa agggatgggg ccgacggcga ctgcccggca
     7081 ggcaggcggg atcctgcgcg ggagcgccgg aaccgggcgc gggctccggg aggccgcacg
     7141 cccgctcatg gctgcaggag catgggggcg tcgcgatcag ccgtacacgg actggaaggc
     7201 cggctcgtgt tccggcagcg gccggcgctg gccctcgcca tcgacggcga cgtagacgat
     7261 c
//
LOCUS       DAOQJH010000027         7178 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_5771_length_7178_cov_4.471571, whole genome shotgun sequence.
ACCESSION   DAOQJH010000027 DAOQJH010000000
VERSION     DAOQJH010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 7178)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7178)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7178
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5771_length_7178_cov_4.471571"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..290
                     /locus_tag="PLQ18_01210"
     CDS             <1..290
                     /locus_tag="PLQ18_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017366276.1"
                     /note="together with moaC, is involved in the conversion
                     of a guanosine derivative (GXP) into molybdopterin
                     precursor Z; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HNE00295.1"
                     /translation="YRVGGSASRIGFISPHSHNFCGDCNRVRVSAEGQLLLCLGQEHS
                     VDLRHVLRAHPGDPERLKQAIVEAMHIKPKGHDFNLKAQPVILRHMNMTGG"
     gene            390..857
                     /locus_tag="PLQ18_01215"
     CDS             390..857
                     /locus_tag="PLQ18_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002692109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNE00296.1"
                     /translation="MQRSLYIDPQKCTGCLQCEMACSYENEQAFNPSKSRIKVFAFHE
                     EGRFVPYTCTQCAEAWCLHACPVEAITKNPATGAMEVNEKTCVGCKVCTIACPFGTVN
                     YNTATGKVIKCDLCQGDPACAKACPTGAITYVDSSWTGLDKMRAWAAKANTAA"
     gene            877..2733
                     /locus_tag="PLQ18_01220"
     CDS             877..2733
                     /locus_tag="PLQ18_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HNE00297.1"
                     /translation="MGWNNKVLRVNLSTGSMSVEPLRMDWARDYLGQRGLATKYLVEE
                     TDPKVDPLSPDNKMIMTTGPLTGTEASTSGRYSVVTKGPLTNAIACSNSGGFFGAELK
                     YAGWDMIIFEGRSPKPVYLSIVDDKAELLDAAEIWGKSVWETEHWVHKKHHDPLVRVA
                     TIGAGAEKGVLYAAIVNDLHRAAGRSGVGAVMGSKNLKAVVVRGTIGMNKVKDPVAFH
                     AASEAAKKVLADNAVTGAGLPKYGTQVLMNVINEIGAMPTRNWREVQFEGASAISGEA
                     MYEPRQSDGKPNLVRNGACFGCTIACGRISTIDPTHFSVQGEGKERYKHASGGLEYEA
                     AWALGSDTGIDDLDALTYANFLCNEHGLDPISFGATVAAAMELYEVGALTKEQTGGIE
                     FKFGNAEALCKAAELTGKGEGFGLELGLGSRRLCEKYGHPEFSMTVKSQEFPAYDARG
                     IQGMGLTYATSNRGACHLRSYTVASEVLGIPVKTDPLVTDGKPELVKAFQDATAVVDS
                     TGLCVFTTFAWTLADFQPQIQAACEGDWSMEKLNEVGERIWNLERQYNLDAGLTAADD
                     TLPKRLLTEAAKTGPAAGRVNDLGKMMPVYYELRGWTADGKLKPETRKRLGV"
     gene            2807..4120
                     /locus_tag="PLQ18_01225"
     CDS             2807..4120
                     /locus_tag="PLQ18_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002692103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNE00298.1"
                     /translation="MIHRYVIVGAGPAGVIAAETLRTTDPDADITLIGDEPERPYSRM
                     AIPYLLIEKIGEEGTHLRKGNDHYDSKGIRVLRDRVEKVDAKTKRLTLKSGGAMDYDK
                     LLVCTGSRAVRPPVPGFDLPGVHTCWTLEDARNIARLAEKGAKVVLVGAGFVGCIVLE
                     ALALRGVDLTVLEMGDRMVPRMMSPIAGGLIKSWCEKKGVRVITSTRVTGITENGGLM
                     NKLVNTLTGKPTRALKVTLNTGETLDADLVVSAAGVAPNIEFLQGCGLEIERGILIDH
                     YMQTNVPDIYAAGDVAQGRDFSTGGLEVHAIQPTASEHGRTAGLNMAGVPTYYRGSFN
                     MNVLDTLGLISSSFGLWMGVEGGDRVEMCEPDRFRYLQLQFDGDIMVGATSLGLTQHV
                     GVLRGLIQGKVPLGPWKAKLMDDPTRIMEAYLACNFAARVAPKAA"
     gene            4129..4380
                     /locus_tag="PLQ18_01230"
     CDS             4129..4380
                     /locus_tag="PLQ18_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018229610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HNE00299.1"
                     /translation="MQITLKLYATLTPHLPAGARRNVATLEVADGASVAGVIDDLHLP
                     PALVHLVLVNGSFVGPEDRAARVLRAGDALAVWPPVAGG"
     gene            4373..4678
                     /locus_tag="PLQ18_01235"
     CDS             4373..4678
                     /locus_tag="PLQ18_01235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE00300.1"
                     /translation="MAEPSALLWQGEMALDRREFLRVLPAACAGRPHAQAGDAIRVEL
                     GAGARLRIELGPTRERRIALLALPATVITILADGPVPAALASAFLRDFENAYRRGGG"
     gene            complement(4683..5063)
                     /locus_tag="PLQ18_01240"
     CDS             complement(4683..5063)
                     /locus_tag="PLQ18_01240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE00301.1"
                     /translation="MNLLPARLRWIAVLPALLPSLPALAGEPLEVYVARLSAADHYNS
                     SGERLRSAAAIIRQDRANLHRYGVRDPEDETDRFFAAADNRARLERLLADGFLDRTTE
                     RIIVEDTPLVRVEVWPNHVEVRLK"
     gene            complement(5120..5590)
                     /locus_tag="PLQ18_01245"
     CDS             complement(5120..5590)
                     /locus_tag="PLQ18_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HNE00302.1"
                     /translation="MTPARPRRLLIVFHSQSGRTARLAAAVAAGARECGGIETLLRRA
                     PEAGLDDLLGCDGLLLGTPENFGYMSGLLKDFFDRTYYPAEGRTVGLPYAVFVSCGND
                     GSGAVREIGRIARGYGWKEVAEALIVRGEPDDEALQRAHELGAALAAGLELGIF"
     gene            6050..6418
                     /locus_tag="PLQ18_01250"
     CDS             6050..6418
                     /locus_tag="PLQ18_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018914341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE00303.1"
                     /translation="MSKTILIIDDSPSLRQIVAYTLTQAGHAVVEAASGDEALQRLDG
                     RVLNLVICDLHMPGINGLEFVAKLKARHEYRFVPVLMLTTEASDTCREAGRAAGAKAW
                     MVKPFQPAALCAAVTRLLAA"
     gene            6427..6741
                     /locus_tag="PLQ18_01255"
     CDS             6427..6741
                     /locus_tag="PLQ18_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005905535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNE00304.1"
                     /translation="MLHIDGNGRRLTLAGRFTIYHVAELLAYLRGLELAPMLEIDLSG
                     VTDFDSAGVQLLLALQREQQQHGRALRLLQHSAAVLDVFTLYELSAHFGDPVLLPAGG
                     GA"
     gene            6738..>7178
                     /locus_tag="PLQ18_01260"
     CDS             6738..>7178
                     /locus_tag="PLQ18_01260"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HNE00305.1"
                     /translation="MNLPLQAVMQTFCIEARERIAELEAGLLTLEQAPSDTEALSAVF
                     RAAHTIKGSAGMLGLDAVSAFTHGFEDVLDALRTGARRFEHRLGELLLLAADHLALLL
                     DAAEAGEVPDAALQAAGTALLQALHEEPQASAVPALPPAPVPMPV"
BASE COUNT         1202 a         2446 c         2434 g         1096 t
ORIGIN      
        1 actaccgcgt cggcggcagt gcgagccgca tcggcttcat ctcgccgcac agccacaact
       61 tctgcggcga ctgcaaccgc gtgcgcgtgt cggccgaggg ccagctgctg ctctgcctcg
      121 gtcaggagca ttcggtcgac ctgcgccacg tgctgcgcgc ccatcccggt gacccggagc
      181 gcctgaagca ggcgatcgtc gaggcgatgc acatcaagcc caagggccac gatttcaacc
      241 tgaaggcgca gcccgtgatc ctgcgccaca tgaacatgac gggcggctga acccgccgca
      301 gcgtgccggc aaccgccgtg aatgcggtga attgccgatc cgagtaccac tggaccatca
      361 caaaccatca agagagagag ggcctgtcga tgcaacgatc cctgtacatc gacccgcaga
      421 agtgcaccgg atgcctgcag tgcgagatgg cctgctccta cgagaacgag caggccttca
      481 atccctcgaa gtcgcgcatc aaggtgttcg cgttccacga ggaaggccgc ttcgtgccct
      541 acacctgcac gcagtgcgcc gaagcctggt gcctgcatgc ctgcccggtc gaagccatca
      601 ccaagaaccc ggccaccggc gcgatggagg tcaacgagaa gacctgcgtc ggctgcaagg
      661 tctgcacgat cgcctgtccg ttcggcaccg tgaactacaa caccgccacc ggcaaggtca
      721 tcaagtgcga cctctgccag ggcgacccgg cctgcgccaa ggcctgcccg accggcgcga
      781 tcacctacgt ggattcgagc tggaccggac tcgacaagat gcgtgcctgg gccgcgaagg
      841 ccaacacggc tgcctgagcg caggggagac tgagagatgg gctggaacaa caaggttttg
      901 cgcgtcaacc tgagcacggg ctcgatgtcg gtcgagccgc tgcgcatgga ctgggccagg
      961 gactacctcg gccagcgcgg tctggcgacc aagtatctgg tcgaggagac cgatccgaag
     1021 gtcgatccgc tgtcgcccga caacaagatg atcatgacca ccggtccgct gaccggcacc
     1081 gaggcctcga ccagcggtcg ctactcggtg gtcaccaagg gcccgctgac caacgcgatc
     1141 gcctgctcga actccggcgg cttcttcggc gccgagctca agtacgccgg ctgggacatg
     1201 atcattttcg aaggcaggtc gccgaagccg gtgtacctgt cgatcgtcga tgacaaggcc
     1261 gagctgctcg acgccgccga gatctggggc aagtcggtct gggagaccga gcactgggtg
     1321 cacaagaagc accacgatcc gctggtgcgc gtcgcgacca tcggtgccgg tgccgagaag
     1381 ggcgtgctgt acgcggcgat cgtcaacgac ctgcaccggg cggccggccg ctccggcgtc
     1441 ggcgcggtca tggggtcgaa gaacctcaag gccgtcgtcg tgcgcggcac gatcggcatg
     1501 aacaaggtca aggatccggt cgcgttccac gccgcctccg aagccgccaa gaaggtgctg
     1561 gccgacaacg ccgtcaccgg cgccggcctg ccgaagtacg gcacgcaggt gctgatgaac
     1621 gtcatcaacg agatcggtgc gatgccgacc cgcaactggc gcgaagtcca gttcgagggc
     1681 gcctcggcga tctccggcga ggccatgtac gagccgcgcc agagcgacgg caagccgaac
     1741 ctggtgcgca acggcgcctg cttcggctgc acgatcgcct gcggccgcat ctcgaccatc
     1801 gacccgacgc acttctcggt gcagggcgag ggcaaggagc gctacaagca cgcctccggc
     1861 ggcctcgaat acgaagccgc gtgggcgctc ggctccgaca ccggcatcga tgatctcgat
     1921 gcgctgacct acgcgaactt cctctgcaac gagcacggcc tcgatccgat ctccttcggt
     1981 gcgaccgtcg ccgcggcgat ggagctgtac gaagtcggcg cgctgaccaa ggagcagacg
     2041 ggcggcatcg agttcaagtt cggcaacgcc gaggcgctct gcaaggcggc cgagctgacc
     2101 ggcaagggcg agggcttcgg cctcgaactc ggcctcggct cgcggcgcct gtgcgagaag
     2161 tacggccatc cggaattctc gatgaccgtc aagagccagg aattcccggc ctacgacgcg
     2221 cgcggcatcc agggcatggg cctgacctat gcgacctcga accgcggcgc ctgccacctg
     2281 cgcagctaca cggtcgcctc cgaggtgctc ggcatcccgg tcaagaccga tccgctggtc
     2341 accgacggca agccggaact ggtcaaggcc ttccaggacg cgaccgccgt cgtcgattcg
     2401 accggcctgt gcgtgttcac gaccttcgcg tggacgctcg cggacttcca gccgcagatc
     2461 caggccgcct gcgaaggcga ctggagcatg gagaagctca acgaggtcgg cgagcgcatc
     2521 tggaacctgg agcgccagta caacctcgat gccggcctga ccgctgccga tgacacgctg
     2581 ccgaagcgcc tgctgaccga agccgcaaag accggcccgg ctgccggccg cgtcaacgat
     2641 ctcggcaaga tgatgccggt gtactacgag ctgcgcggct ggaccgccga cggcaagctg
     2701 aagcccgaga cgcgcaagcg gctcggcgtc tgagacgcca gcaccctgcg caacggcccg
     2761 gccaatcgcc gggccgttcg ttcgaacggc ttgcgaggaa tactccatga tccaccgcta
     2821 cgtcatcgtc ggtgccggcc cggccggcgt catcgccgcc gaaaccctgc gcacgaccga
     2881 tcccgacgcc gacatcacgc tgatcggcga cgaacccgaa cgcccgtact cgcgcatggc
     2941 gattccctac ctgctgatcg agaagatcgg cgaggaaggc acgcacctgc gcaagggcaa
     3001 cgatcactac gattccaagg gcatccgcgt cctgcgcgac cgggtcgaga aggtcgatgc
     3061 caaaacgaag cgcctgacgc tgaagtccgg cggcgcgatg gactacgaca agctgctggt
     3121 ctgcaccggc tcgcgtgccg tgcgtccgcc ggtgccgggt ttcgacctgc ccggcgtgca
     3181 cacctgctgg acgctcgagg acgcccgcaa catcgcccgg ctcgccgaga agggcgcgaa
     3241 ggtggtgctg gtcggcgccg gcttcgtcgg ctgcatcgtg ctcgaagccc tggcgctgcg
     3301 cggtgtcgat ctgaccgtgc tcgaaatggg cgaccgcatg gtgccgcgca tgatgagccc
     3361 gatcgccggc ggcctgatca agagctggtg cgagaagaag ggcgtgcgcg tgatcacctc
     3421 gacgcgcgtg accggcatca ccgaaaacgg cggcctgatg aacaagctgg tcaacacgct
     3481 gaccggcaag ccgacgcgcg cgctgaaggt cacgctgaac accggcgaaa ccctcgatgc
     3541 cgatctggtc gtctcggccg ccggtgtcgc cccgaacatc gagttcctgc agggctgcgg
     3601 gctggagatc gagcgcggca tcctgatcga tcactacatg cagaccaacg tcccggacat
     3661 ctatgcggcc ggcgacgtgg cgcagggccg cgacttctcg accggtggtc tcgaagtgca
     3721 tgcgatccag ccgaccgcct ccgagcacgg ccgcaccgcc ggcctgaaca tggccggggt
     3781 gccgacgtat taccgcggca gcttcaacat gaacgtgctc gacaccctcg gcctgatctc
     3841 cagctccttc gggctgtgga tgggcgtcga ggggggcgat cgcgtcgaga tgtgcgagcc
     3901 cgatcgcttc cgctacctgc agctgcagtt cgatggtgac atcatggtcg gcgcgacctc
     3961 gctcggcctg acgcagcacg tcggcgtgct gcgcgggctg atccagggca aggtgccgct
     4021 cggcccgtgg aaggcgaagc tcatggatga cccgacgcgg atcatggagg cgtatctcgc
     4081 ctgcaacttc gccgcccgcg tggcgcccaa ggctgcctga gaccgacgat gcagatcacg
     4141 ctgaagctgt atgcgacgct gaccccgcac ctgcccgccg gcgcccggcg caacgtcgcg
     4201 acgctggaag tcgcggacgg tgccagcgtc gccggcgtca tcgacgatct gcatctgccg
     4261 ccggccctcg tgcatctggt gctggtcaac ggcagcttcg tcggccccga ggaccgggcc
     4321 gcgcgcgtgc tgcgcgccgg cgacgcgctc gcggtctggc cgccggtcgc cggtggctga
     4381 gccttcggcc ctgctgtggc agggggagat ggcgctcgac cggcgcgagt tcctgcgcgt
     4441 gctgccggcc gcctgtgccg gccggccgca cgcgcaggcc ggcgatgcga tccgggtcga
     4501 gctcggtgcc ggtgcaaggc tgcgcatcga actcggcccg acccgcgagc ggcgcatcgc
     4561 gctgctcgcg ctgccggcca ccgtcatcac catcctcgcc gacggtccgg tgcccgcggc
     4621 actggcgagt gccttcctgc gcgatttcga gaacgcctac cggcgcggcg gcggctgaag
     4681 gttcacttca gccgcacttc gacgtggttg ggccagacct cgacccgcac cagcggcgtg
     4741 tcctcgacga tgatccgttc ggtcgtgcgg tccaggaagc cgtcggcgag caggcgttcg
     4801 agccgggcgc ggttgtcggc cgcggcgaag aagcggtcgg tttcgtcctc cggatcgcgt
     4861 acgccgtagc ggtgcaggtt ggcgcgatcc tggcggatga tcgccgcagc cgagcgcagg
     4921 cgctcgccgc tgctgttata gtgatcggcc gcactcaggc gcgcgacgta gacctccagg
     4981 ggttccccgg cgagggccgg cagcgacggc agcagggccg gcagcacggc gatccagcga
     5041 aggcgggcag gcagcaggtt catgtcggga aagctccgct cgggtcaagg gcagcggccg
     5101 cacatgccgg ccggcggttt cagaaaatcc ccagttccag ccccgcagcc agcgccgcgc
     5161 cgagttcgtg cgcgcgttgc agcgcctcgt cgtccggctc gccgcgcacg atcagcgcct
     5221 cggccacttc cttccagcca tagccgcggg cgatgcgtcc gatctcgcgc accgcgccgc
     5281 tgccgtcgtt gccgcagctg acgaacaccg catacggcag cccgacggta cggccctcgg
     5341 ccgggtagta ggtacggtcg aagaagtcct tcaggagccc ggacatgtag ccgaagttct
     5401 cgggcgtgcc gagcagcagg ccgtcgcagc cgagcagatc gtcgaggccg gcctcgggcg
     5461 cacgcctgag cagcgtttcg atgccgccgc attcgcgtgc gccggccgcg acggcagcgg
     5521 cgaggcgcgc cgtgcggccg ctctggctgt ggaacacgat cagcaggcgc cgggggcggg
     5581 cgggagtcac ggcgggagtc cggcacaggg cagggacgtg gagcgtagcg ccgtccggcc
     5641 cggctgtccc ggaggccggg ttcaggccgg cgtccggcag cgattcgccc atgccatcgc
     5701 tgcggcattt ccgcggcggc cggtccggcc ggatatagcc ggatgcccat ggacagcgcc
     5761 gcaaataccc ggattcattc gcacgattgc gcagcctgcg gattcgtccg gcaggaagtg
     5821 ccggtttttt gttatcggtt atcgtcacga acatcccctg aatcaggtgc cggaattccg
     5881 gcgcttctga taattgctgt gagatgccgg tcagtactgg tgtcggctgc tggcacgaat
     5941 ccgtgcggtg gtcacaatgc ggcatttcgt cgacggtgca gtcctcaagg aattgccgga
     6001 gccgtcgaaa ttcagattgc tgaacattca tcgccccagg gacatgacga tgtcgaaaac
     6061 cattctcatc atcgatgact cgccgtcctt gcgccagatc gtggcctaca cgctgaccca
     6121 ggccggccac gcggtcgtcg aggctgcgag cggcgacgag gccttgcagc ggctcgacgg
     6181 gcgcgtgctg aacctggtca tctgcgacct gcacatgccg ggcatcaacg gcctggagtt
     6241 cgtcgcgaaa ctcaaggccc gccacgaata tcgcttcgtg ccggtgctga tgctgaccac
     6301 cgaggcgagc gacacctgcc gcgaggccgg gcgtgccgcc ggggccaagg cctggatggt
     6361 gaagcccttc cagccggcgg cgctgtgcgc ggcggtcacg cggctgctgg ccgcctgagc
     6421 cacgccatgc tgcacatcga cggcaatggc aggcggctga cgctggccgg gcgattcacg
     6481 atctatcacg tcgcggaact gcttgcgtat ctgcgcggcc tggaactggc gccgatgctg
     6541 gaaatcgacc tgtccggcgt gaccgatttc gacagtgccg gcgtgcaatt gctgctggcc
     6601 ctgcagcggg aacagcagca gcacggccgc gcattacgcc tgctccagca cagtgctgcg
     6661 gtgctcgacg tgttcacgct gtatgagctg tccgcgcatt tcggggatcc ggtcctgctg
     6721 ccggccggcg gtggcgcatg aacctgccgc tgcaggccgt gatgcagacc ttctgcatcg
     6781 aggcccgcga acggattgcg gagctcgaag ccgggctgct gaccctcgaa caggcgccct
     6841 ccgataccga ggccctgagt gcggtgttcc gcgccgcgca cacgatcaag ggctcggccg
     6901 gcatgctcgg gctggatgcg gtttcggcat tcacgcacgg cttcgaggac gtgctcgatg
     6961 cgctgaggac cggcgcgcgc cgcttcgagc acaggctcgg cgaactgctg ctgctcgccg
     7021 ccgatcatct ggcgctgctg ctcgatgccg ccgaggccgg cgaagtgccg gatgcggcgc
     7081 tgcaggccgc cggcacggcg ctgctgcagg cgctgcacga agagccgcag gcatcggcgg
     7141 tccctgcgct cccgccggca ccggtcccga tgccggtc
//
LOCUS       DAOQJH010000028         7146 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_5809_length_7146_cov_4.244958, whole genome shotgun sequence.
ACCESSION   DAOQJH010000028 DAOQJH010000000
VERSION     DAOQJH010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 7146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7146
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5809_length_7146_cov_4.244958"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..510)
                     /locus_tag="PLQ18_01265"
     CDS             complement(<1..510)
                     /locus_tag="PLQ18_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018026141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNE00306.1"
                     /translation="MKLKLNNLALAAAVAIAVAAPAFADPEPAVTDPFGKAVKDPFDK
                     CVLAVGGTVLPECGPMVQKKRSELKLSGDTNFDFNKDTLKPAGKAELDKLVADMKQAK
                     QVDAVIVVGHTDSVGSDAYNQRLSERRAATVKNYLAAKGVPAGIIQASGRGESQPVAD
                     NKTKKGREMN"
     gene            734..1432
                     /locus_tag="PLQ18_01270"
     CDS             734..1432
                     /locus_tag="PLQ18_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018604555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNE00307.1"
                     /translation="MRVALLEDDRPQAEMMRTWLVEAGHDVQVFVQGKALVRELGRDS
                     YDLVVLDWMLPDIDGIQVLKWIRTHVDWPMPVLFVTGKDEEDDIVQALDNGADDYMVK
                     PVKRMEMLARLKAVSRRAIAQDEGQKILEFGVYTVDLTTRAVLLNGQPIELTQKEFDL
                     TVFLFRGAGRVLSRGHILESVWGRSPDLNTRTVDTHVSRIRNKLDLRPEHGWQLKAVY
                     QHGYRLEPVEVPAT"
     gene            1568..2629
                     /gene="mtnA"
                     /locus_tag="PLQ18_01275"
     CDS             1568..2629
                     /gene="mtnA"
                     /locus_tag="PLQ18_01275"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006786243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HNE00308.1"
                     /translation="MSVLSDPAAHDRVRAVAWRDGALELLDQRLLPAREQRLRFDDAA
                     AVARAIRDMVVRGAPAIGIAAAYGVVLAARARFAADGEAWTQTIAADLERLADARPTA
                     VNLFWAIARMRRRIDALPAGVDPLPALLAEADAILAEDIAANRRMGELGAALIEPGRA
                     LLTHCNTGSLATGGYGTALGVIRSAWAGGRLTAVCADETRPWLQGARLTAWELVQDGI
                     PVTLIAEGAAAHRMKQGDIGWVVVGADRITADGSAANKIGTYGLAVLARFHGVRFMVV
                     APSSTIDMSLASGGDIPIEMRAPAELLELGGTRVAAEGAQAWNPSFDVTPAALIDAIV
                     TERGVVLAPDAAKMAALMA"
     gene            complement(2684..3409)
                     /locus_tag="PLQ18_01280"
     CDS             complement(2684..3409)
                     /locus_tag="PLQ18_01280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE00309.1"
                     /translation="MNYRDPELRELLSAQYVLGTLKPRARRRFESLLPGRADLRRSVA
                     GWERRLPELLGPTPAIEAPDSVWPKLRTRLFPETLAAAPATTASPAPGLLARLSFWRG
                     WAAMASVVAFVFALALGLGKQTAIPGYASVVTSMQSHEPMWMLSAAPEMDRIYVKSIQ
                     PMVMSPDIGCLLWIRPEGSERMILVGELPWRGEEATLKIDKATRAQLKGQLVVTVERV
                     DRGMPDKPTAAPKFAGSWMPIRI"
     gene            complement(3406..3963)
                     /locus_tag="PLQ18_01285"
     CDS             complement(3406..3963)
                     /locus_tag="PLQ18_01285"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNE00310.1"
                     /translation="MSVPEVLEDLLARTALADRHAFEKLYRLSSAQLFGIVLRIVRDR
                     DLAADVLQETYVKIWHRAGDFRADLAQPLTWMGSIARHQAIDTLRRSARRLSTAEDID
                     EHYELADEAAESPADAVDRGLQGAALHRCLGELQGLQQRAVQLAWFRGLTHEDIAARL
                     DTPLGTVKSWLRRGLLRLKTCLEGA"
     gene            complement(3960..4430)
                     /locus_tag="PLQ18_01290"
     CDS             complement(3960..4430)
                     /locus_tag="PLQ18_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018719289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1841 family protein"
                     /protein_id="HNE00311.1"
                     /translation="MFGTDRNQLRRYYCETWRRHRAGETLEPLETLIATVIEAHPEYQ
                     ALLADPERALADEWTPQRGESNPFLHMGMHLGIQEQVQTDRPAGIRAAWLALARKLGD
                     PHAAEHAMMEALGEALWEAQRFGAAPDEAKYLARVQALAGIGPGPFPGSAGPRA"
     gene            complement(4431..5069)
                     /gene="nth"
                     /locus_tag="PLQ18_01295"
     CDS             complement(4431..5069)
                     /gene="nth"
                     /locus_tag="PLQ18_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019399277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HNE00312.1"
                     /translation="MTPPQRREFFSRLQAANPHPTTELQYASPFELLVAVILSAQATD
                     KSVNKATARLFPVANTPAQMLALGEDALRAHVQTIGLYRNKAANILKTCRLLLERHDG
                     EVPRTRAALEALPGVGRKTANVILNTAFGEPVIAVDTHIFRVANRTGLAPGRDVLAVE
                     RELERVVPDEYRQDAHHWLILHGRYVCTARRPQCPACVVRDLCACPDKTGDA"
     gene            complement(5144..5851)
                     /locus_tag="PLQ18_01300"
     CDS             complement(5144..5851)
                     /locus_tag="PLQ18_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005965740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HNE00313.1"
                     /translation="MSASAPSKSEILANGLWRNNTGLVALLGLCPLLATSSSAVNGLG
                     LGLATVLVLLLTNTTIALIRSFVPNEVRIPVFIVVIASAVTAVELAMHAFLPELHKVL
                     GIFIPLIVTNCCVLGRAESFASKNAPALAALDGVATGLGFTLVLTALGSLREFVGQGT
                     LFASADIMFGEAGRMLTVQVLHDYRGFLLAILPPGAFIGLALLIAGKNWLDARTNART
                     AATPVEAGCTPEAHARA"
     gene            complement(5848..6489)
                     /gene="rsxG"
                     /locus_tag="PLQ18_01305"
     CDS             complement(5848..6489)
                     /gene="rsxG"
                     /locus_tag="PLQ18_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002707248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HNE00314.1"
                     /translation="MSEVLQQMRRGGGVLGGFALLSVALVAATYFGTRGRIAASEQAA
                     LEAQLNALVPAARYDNHPTEDTIRLSDAAGFGSGEAVTVYRARRAGEPVAALATVIAP
                     DGYAGPIRLLVGVWADGRIAGVRVLAHKETPGLGDRIEIQRSDWIRSFDERSLTDPDA
                     AGWKVRKDGGRFDQFTGATITPRAIVKAVHGFLEYAQAHRAELYAPASGDRAP"
     gene            complement(6495..>7146)
                     /locus_tag="PLQ18_01310"
     CDS             complement(6495..>7146)
                     /locus_tag="PLQ18_01310"
                     /inference="COORDINATES: protein motif:HMM:TIGR01946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HNE00315.1"
                     /translation="VVLLISFPLEMSTWAAPRGVGGHVAGFGEALALIFGGHGDHVLL
                     DAVSSATPLDTLHTQLGLGQTVAAVRAGSPVFGWIGGSGWEWANLAFLGGGLWMCQRR
                     VAAWQIPAGMLGALALISTVFWIAEPARFAPPWFHVMSGAAVFGAFFIATDPVSASTT
                     PRGRLIYGAGIGLLTWLIRSLGGFPDGVAFAVLLMNLAAPTIDQYTLPRAFGAGRA"
BASE COUNT         1031 a         2421 c         2635 g         1059 t
ORIGIN      
        1 gttcatctcc cggccctttt tggtcttgtt gtcggcaacc ggctgcgact cgccgcggcc
       61 cgaggcctgg atgatgccgg ccgggacgcc cttcgcggcc aggtagttct tgacggtcgc
      121 ggcgcgacgc tcggacaggc gctggttgta ggcatcggag ccgacgctgt cggtgtgacc
      181 gacgacgatg acggcatcga cctgcttggc ctgcttcatg tcggcgacga gcttgtcgag
      241 ctcggccttg ccggcgggct tcagcgtgtc cttgttgaag tcgaagttcg tgtcacccga
      301 aagcttcagt tccgagcgct tcttctgcac catcgggccg cattccggca gcacggtgcc
      361 gccgacggcg agcacgcact tgtcgaacgg gtccttgacc gccttgccga acgggtcggt
      421 gacggccggc tcggggtcgg cgaaggccgg ggccgcaact gcgatggcaa cggccgcggc
      481 cagggcaagg ttgttaagct taagtttcat cgtctaacct ccaagtcact gcaagcaaag
      541 tccggaacgg tctaaccagc cggcgttgac acttcttctc gggatggttt cggaaggggc
      601 ggtcggcatc ctttgctacc ggctcccaag tcgggccgag tataacggaa aaatcggttt
      661 tcctagtttc ggatgataca cccctgtgtc agaattgcaa aaagtgttgc gattggaata
      721 aagggagagt gccttgcgcg tcgcactgct tgaggatgac cgcccccagg cggagatgat
      781 gcggacgtgg ctggtcgagg ccggccacga cgtacaggtg ttcgtccagg gcaaggccct
      841 ggtccgcgaa ctcggccgtg attcctacga tctggtcgtg ctcgactgga tgctgccgga
      901 catcgacggc attcaggtgc tcaagtggat ccgcacgcac gtcgactggc cgatgccggt
      961 gctgttcgtg accggcaagg acgaggagga cgacatcgtg caggcgctcg acaacggcgc
     1021 cgacgactac atggtcaagc cggtcaagcg catggagatg ctggcgcggc tgaaggcggt
     1081 gtcgcgccgg gcgatcgcgc aggacgaggg gcagaagatc ctcgagttcg gcgtctacac
     1141 ggtcgacctg accacgcgcg ccgtgctgct gaacggccag ccgatcgagc tgacgcagaa
     1201 ggaattcgac ctgaccgtgt tcctgttccg gggcgccggg cgcgtgttgt cgcgcggcca
     1261 catcctggag agcgtctggg gccgcagccc cgacctcaac acgcgcacgg tcgacacgca
     1321 cgtcagccgc atccgcaaca agctcgacct gcgccccgag cacggctggc agctgaaggc
     1381 cgtctaccag cacggctacc ggctggaacc ggtcgaagtc ccggccacct gagccccggc
     1441 cgcaggaccc ccgctgcccg atgcgggccg cgggggtttt tcgtgcccga cggatcgcta
     1501 cactggcgcc cctccatcga accggccgcc tgccgcggcc cttccggtag tccggggagt
     1561 cctgccgatg tccgtcctgt ccgaccctgc cgcgcatgac cgcgtccgtg ccgtcgcctg
     1621 gcgcgacggt gcgctcgaac tgctcgacca gcgcctgctg ccggcccggg agcagcggct
     1681 gcgcttcgac gatgccgcgg ccgtcgcgcg cgcgatccgc gacatggtcg tgcgcggtgc
     1741 gccggcgatc ggcatcgccg cggcctacgg cgtcgtgctc gcggcccgcg cgcgcttcgc
     1801 ggccgatggc gaggcgtgga cgcagacgat tgcggccgat ctcgaacggc tcgccgatgc
     1861 gcgcccgacc gcggtcaacc tgttctgggc gatcgcgcgc atgcgcaggc gcatcgacgc
     1921 gctgccggcc ggcgtcgatc cgctgccggc gctgctcgcc gaggccgacg ccatcctcgc
     1981 cgaggacatc gccgccaacc ggcgcatggg cgagctgggc gcggcgctga tcgagccggg
     2041 ccgggcgctg ctcacgcact gcaacaccgg ctcgctcgcc accggcggct acggcaccgc
     2101 gctcggcgtg atccgcagcg cctgggccgg cgggcggctc acggccgtct gcgccgacga
     2161 gacgcggccg tggctgcagg gcgcgcggct gacggcctgg gagctggtgc aggacggcat
     2221 cccggtgacg ctgatcgccg agggcgcggc cgcgcaccgc atgaagcagg gcgacatcgg
     2281 ctgggtcgtg gtcggtgccg accgcatcac cgccgacggc tcggccgcca acaagatcgg
     2341 cacctacgga ctcgccgtgc tggcgcgctt ccacggcgtg aggttcatgg tcgtggcgcc
     2401 gagctcgacg atcgacatga gcctcgcgag cggcggggac atcccgatcg agatgcgtgc
     2461 ccccgcggag ctgctcgaac tcggcggcac gcgcgtcgcg gccgaaggcg cgcaggcctg
     2521 gaatccgtcc ttcgacgtga cgccggcggc gctgatcgac gcgatcgtca ccgagcgcgg
     2581 cgtggtgctc gcgccggatg ccgcgaagat ggcggcgctg atggcctgaa gggtcgcgga
     2641 aggcctgcag cgggcctgcc ttccgcgccc tgccggggtg ccttcagatg cggatcggca
     2701 tccagctgcc ggcgaacttc ggtgccgcgg tcggcttgtc gggcatgccg cgatcgacgc
     2761 gctcgacggt gacgacgagc tggcccttga gctgcgcgcg cgtcgccttg tcgatcttca
     2821 gcgtcgcctc ctcgccgcgc cacggcagtt cgccgacgag gatcatgcgc tccgagccct
     2881 cgggccggat ccacagcagg cagccgatgt ccggcgacat gaccatcggc tggatgctct
     2941 tgacgtagat gcggtccatc tccggggccg ccgacagcat ccacatcggt tcgtgggact
     3001 gcatgctggt gacgacgctg gcgtagccgg ggatcgcggt ctgcttgccg aggccgagcg
     3061 ccagcgcgaa cacgaaggcg acgacactgg ccatcgccgc ccagccgcgc cagaagctca
     3121 ggcgcgcgag caggccgggc gcaggcgaag cggtcgtcgc cggggccgcc gcgagcgttt
     3181 ccgggaacag gcgcgtgcgc agcttcggcc agacgctgtc cggtgcctcg atcgccggcg
     3241 tcgggccgag cagttccggc agccggcgtt cccagccggc gacgctgcgg cgcaggtcgg
     3301 cgcggccggg cagcaggctc tcgaagcgcc ggcgcgcgcg cggtttcagt gtgccgagca
     3361 cgtactgcgc cgacagcagt tcacggagtt cggggtcgcg gtagttcatg cgccctccag
     3421 acaggtcttg agccgcagca ggccgcggcg cagccaggac ttgacggtac cgagcggcgt
     3481 gtccagccgc gcggcgatgt cctcgtgcgt gaggccgcga aaccacgcga gctgcacggc
     3541 gcgctgctgc aggccctgca gctcgccgag gcagcggtgc agggccgcac cctgcaggcc
     3601 gcgatcgacg gcgtcggccg gactctcggc cgcctcgtcg gcaagctcgt agtgctcgtc
     3661 gatgtcctcg gccgtgctca ggcgccgggc gctgcggcgc agcgtgtcga tcgcctgatg
     3721 gcgggcgatc gagcccatcc aggtcagcgg ctgcgcgaga tcggcgcgga aatcgccggc
     3781 gcggtgccag atcttcacgt aggtctcctg cagcacgtcg gcggcgaggt cgcggtcacg
     3841 gacgatacgc agcacgatgc cgaacagttg cgccgacgac agtcgataca gcttttcgaa
     3901 tgcatggcga tccgcgagcg ccgtgcgcgc cagcaggtct tcgagcacct cgggcacgct
     3961 catgcacgcg gccccgccga gcccgggaac ggtcccggcc cgatgccggc gagcgcttgc
     4021 acgcgggcga gatacttcgc ctcgtccggc gccgcgccga agcgctgcgc ctcccacagc
     4081 gcctcgccga gggcttccat catcgcgtgc tcggcggcgt gcgggtcgcc gagcttgcgg
     4141 gcgagcgcga gccaggcagc gcggatgccg gccgggcggt cggtctgcac ctgctcctgg
     4201 atgccgaggt gcatgcccat gtgcaggaag ggattgctct cgccgcgctg cggcgtccat
     4261 tcgtcggcga gcgcgcgctc gggatcggcg agcagggcct ggtattccgg atgcgcctcg
     4321 atgacggtgg cgatcagcgt ttcgagcggt tcgagggtct cgccggcgcg gtgccggcgc
     4381 caggtctcgc agtagtagcg gcgcagctgg ttgcggtcgg tgccgaacat tcaggcgtct
     4441 ccggtcttgt cggggcaggc gcagaggtcg cgcaccacgc aggccgggca ttgcggccgg
     4501 cgcgcggtgc agacgtagcg gccgtgcagg atcagccagt ggtgcgcgtc ctgacggtat
     4561 tcatcgggca cgacgcgctc gagctcgcgc tcgacggcga gcacgtcgcg cccgggggcg
     4621 aggccggtgc ggttggcgac gcggaagatg tgcgtgtcga ccgcgatcac cggctcgccg
     4681 aaggcggtgt tgaggatgac gttcgcggtc ttgcgcccga cgccgggcag ggcttcgagc
     4741 gcggcgcgcg tgcgcggcac ctcgccgtcg tgacgttcca gcagcagccg gcaggtcttc
     4801 aggatgttcg cggccttgtt gcggtacagg ccgatggtct gcacgtgggc gcgcagggcg
     4861 tcctcgccga gcgcgagcat ctgcgccggc gtgttcgcga ccgggaacag ccgggccgtg
     4921 gccttgttga cgctcttgtc agtggcctgc gccgacagga tcaccgccac cagcagctcg
     4981 aacgggctcg cgtactgcag ttcggtggtc ggatgcgggt tcgcagcctg caggcgggag
     5041 aagaattcgc ggcgttgcgg cggggtcatc ggcgggcggg cggcgcggaa aggcgcttat
     5101 cgtagcgcaa gccggccacc ggcccatcat gcgccccggg cgttcaggcg cgggcgtggg
     5161 cctcgggcgt gcagccggct tcgaccgggg tcgcggccgt gcgggcgttg gtacgggcgt
     5221 cgagccagtt cttgccggcg atcagcagcg ccagcccgat gaacgcgccg ggcggcagga
     5281 tcgcgagcag gaagccgcgg tagtcgtgca gcacctgcac ggtcagcatg cggccggcct
     5341 cgccgaacat gatgtcggcg ctcgcgaaca gcgtgccctg accgacgaac tcgcgcaggc
     5401 tgccgagcgc ggtcagcacc agcgtgaagc cgagcccggt cgcgacgccg tcgagcgcgg
     5461 cgagcgccgg ggcgttcttg gaggcgaagg attcggcacg cccgagcacg cagcagttgg
     5521 tgacgatcag cgggatgaag atgccgagca ccttgtgcag ttcgggcagg aaggcgtgca
     5581 tcgccagttc gacggcggtg accgccgagg cgatcacgac gatgaacacc gggatgcgca
     5641 cctcgttcgg cacgaagctg cggatcagcg cgatcgtcgt gttggtcagc agcagcacca
     5701 gcaccgtcgc cagccccagg ccgaggccgt tgaccgccga gctggaggtc gcgagcagcg
     5761 gacacagccc gagcagcgcg accagaccgg tgttgttgcg ccagaggccg ttggcgagga
     5821 tctcggactt gctcggggca ctggcgctca tggggctcgg tctccgctgg ccggggcgta
     5881 gagttcggcg cgatgcgcct gcgcgtattc gaggaagccg tgcacggcct tcacgatcgc
     5941 gcgcggcgtg atggtcgcgc cggtgaactg gtcgaagcgc ccgccgtcct tgcgcacctt
     6001 ccagccggcg gcgtcgggat cggtgaggct gcgctcgtcg aagctgcgga tccagtcgct
     6061 gcgctggatc tcgatgcgat cgccgagccc cggcgtttcc ttgtgcgcga gcacgcgcac
     6121 gccggcgatg cggccatcgg cccagacgcc gaccagcagc cggatcggcc cggcgtagcc
     6181 gtcgggcgcg atgacggtcg cgagcgccgc gaccggctcg ccggcgcgcc gcgcccggta
     6241 gacggtcacc gcttcgccgc tgccgaagcc ggcggcgtcg ctgagccgga tcgtgtcttc
     6301 agtgggatgg ttgtcatagc gcgcggcggg caccagcgcg ttcagctgcg cttcgagtgc
     6361 ggcctgttcg gaggcggcga tgcgcccgcg cgtgccgaag taggtggcgg cgaccagcgc
     6421 gacgctgagc agcgcgaaac cgccgagcac gccgcccccg cggcgcatct gctgcaggac
     6481 ctcgctcatg gctttcaggc cctcccggcg ccgaaggcac gcggcagcgt gtactggtcg
     6541 atggtcggtg cggcgaggtt catcagcagc acggcaaacg ccacgccatc cgggaagccg
     6601 ccgagcgagc ggatcagcca ggtcagcagg ccgatgccgg cgccgtagat cagccggccg
     6661 cgcggcgtcg tgctggccga caccggatcg gtggcgatga agaaggcacc gaacaccgcc
     6721 gcgccgctca tcacgtggaa ccacggcggt gcgaagcgcg cgggctccgc gatccagaac
     6781 acggtcgaga tcagcgccag cgcgccgagc atgccggccg ggatctgcca ggcggcgacg
     6841 cgccgctggc acatccacag cccgccgccg aggaaggcga ggttcgccca ttcccagccg
     6901 ctgccgccga tccagccgaa caccggactg ccggcgcgca cggcggcgac ggtctggccg
     6961 aggccgagtt gcgtgtgcag cgtgtcgagc ggggtagccg aactcacggc gtcgagcagc
     7021 acgtgatcgc cgtggccacc gaagatcagg gcgagggcct cgccgaagcc ggcgacgtgt
     7081 ccgccgaccc cgcgcggggc tgcccaggtg ctcatctcca gcgggaagga gatcagcagt
     7141 acgacg
//
LOCUS       DAOQJH010000029         7018 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_5959_length_7018_cov_3.817177, whole genome shotgun sequence.
ACCESSION   DAOQJH010000029 DAOQJH010000000
VERSION     DAOQJH010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 7018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7018
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5959_length_7018_cov_3.817177"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..820
                     /locus_tag="PLQ18_01315"
     CDS             <1..820
                     /locus_tag="PLQ18_01315"
                     /inference="COORDINATES: protein motif:HMM:NF017770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF898 family protein"
                     /protein_id="HNE00316.1"
                     /translation="AWWVMPLFGAALLVGALPFVPALIVSSLRFNYRVTRWRNVAFRF
                     TGSYGGAAVAFLLWPLLGVVSFGLLWPKAIQAQRSYLLNHVHYGNAAFSADIPLWPVY
                     GCSIVASVLLGIVTAVATVLSGLMLARAGLRPQSMADLDHWQLFALVLPTLAYLFGLT
                     LSAAYYRAALRNIVLADVAIGDGHGLRSHVSVLDYIAFATGNLLLTVVTLGLARPWVR
                     VRTARYFATTTTIRFAVPAGAAMAGIAAVTEAAGRRGSTLGDAAGDAFDWDLSL"
     gene            817..1854
                     /locus_tag="PLQ18_01320"
     CDS             817..1854
                     /locus_tag="PLQ18_01320"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HNE00317.1"
                     /translation="MSEAGSLRIAGCHYPAGSAAGQAAELHVSADGALRLEPGGRALG
                     HWRGLRVPERLGRAPRVLGLGDGGGFETGDNAAVDACERRFRGRVAGGRLHALEGRWT
                     IAWLALAGLGFSIFAGFVWGIPALAARVAPWLPASVVVGSSEETLASLDRHVLRPSRL
                     PAARCEALEATRARLAARAGLSGGRLECRRGGAFGANAFALPSGLVVITDEFVELGGD
                     EDELAGVLAHEVAHVEYRHGLRQALQGSLLTLGAALLTGDLANAGSFIAHLPVVLAGL
                     HYSRDFEREADARGAALMRAEGRDPRALARALARLEGAARQKERPAAPAWLSDHPDTQ
                     ERIARIEALAR"
     gene            1983..2426
                     /locus_tag="PLQ18_01325"
     CDS             1983..2426
                     /locus_tag="PLQ18_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017364910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNE00318.1"
                     /translation="MPLPVTPRLTVDAIIELADRPGRPIVLIERRNPPPGWALPGGFV
                     DVGERVEDAAIREAAEETGLDVRLITLLGIYSDPARDARFHTAGVVYVAEATGTPVAA
                     DDARHLDVFDPDGPLPLLAFDHAQILADYRQWRAHGTGAPLRPAH"
     gene            2617..3198
                     /locus_tag="PLQ18_01330"
     CDS             2617..3198
                     /locus_tag="PLQ18_01330"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNE00319.1"
                     /translation="MSAPAELTDLLLASGRGDRPAFERLYRLSSPRLYSLARWLLKRD
                     ALAEEIMQEAYVQIWRDAARFDPARASAMTWMAVIVRHRALDELRRKRPELEPGGGSA
                     GSDEDDAMPEPGVAEADPDAGPLELALADGDRRQLGICLETLSEMQRQSILLAFLRGL
                     THSEVASALAAPLGSVKSWVRRGLEQLKRCLTS"
     gene            3195..3917
                     /locus_tag="PLQ18_01335"
     CDS             3195..3917
                     /locus_tag="PLQ18_01335"
                     /inference="COORDINATES: protein motif:HMM:NF021604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HNE00320.1"
                     /translation="MNYRDPKLGTRLAAEYALGTMGGLARRRFEGLMAADPWLRREVR
                     AWQDDLYGIVELIPERTPPERLWTHIETRLWPVATGRVVPLAPKARPSRLWQVWSGLA
                     TAAALLLAVAVWRAPAPPSPQPLAVPDYVAVVSDRDGRPAWLVRPAPGGGLAVSALAP
                     QALASDRAFELWLLPPGGAPPKSLGLLPADGGARLGVAPERLREIVEGDGLAVSLEPA
                     GGSPTGAPTGPVLYLGKPLPRV"
     gene            complement(3984..4715)
                     /locus_tag="PLQ18_01340"
     CDS             complement(3984..4715)
                     /locus_tag="PLQ18_01340"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008208189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HNE00321.1"
                     /translation="MKATLFVADLHLDAAARPQTLALFEDFIAVRARRAQALYILGDL
                     FEAWIGDDDDGETGERVAAALGSLQRSGVPAHFLHGNRDFLLGPDYAARAGLTLLPET
                     TVIDLYGTPTLLTHGDALCTDDVDYQRFRAQIREPANQARLLALPLAQRRAFAAQLRA
                     DSRSAQADKPAEITDVNPDAVRTLMDAHGVTQLIHGHTHRPALHELVLADGDSGWRVV
                     LGDWYTQGSVLAVSRNGMQLEGLHG"
     gene            complement(4712..5200)
                     /locus_tag="PLQ18_01345"
     CDS             complement(4712..5200)
                     /locus_tag="PLQ18_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005223730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNE00322.1"
                     /translation="MIRIQTSLGVITLELDAEHAPATTANFEQYVRDGFYAGTVFHRV
                     IPNFMVQGGGMLADMSQKSTRAPIRNEAKNGLKNARGSIAMARTSDPHSATAQFFINV
                     KDNGFLDHPGQDGWGYCVFGKVIEGMDVVDAIVAVPTANKRGHQNVPVEPVLIEKMEI
                     VE"
     gene            complement(5251..5751)
                     /locus_tag="PLQ18_01350"
     CDS             complement(5251..5751)
                     /locus_tag="PLQ18_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNE00323.1"
                     /translation="MAVTRVQMVTSHGTIVLELDEEKAPQTVANFVRYADEHFYDGTI
                     FHRVIDNFMIQGGGFTPDLQQKPTHDPIPNEAKNGLKNSRGTIAMARTADPHSATAQF
                     FINVRDNGFLDYPSRDGWGYCVFGKVVDGLEVVERIRQTPTTTVGPYSDVPAEPVVIR
                     SVTVVK"
     gene            5969..>7018
                     /gene="cysS"
                     /locus_tag="PLQ18_01355"
     CDS             5969..>7018
                     /gene="cysS"
                     /locus_tag="PLQ18_01355"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012969673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HNE00324.1"
                     /translation="MSVEHDLQLYNSLSRRKERFVPITPGQVRMYVCGMTVYDYCHVG
                     HARVMVVFDAIVRYLRWLGYDVTYVRNITDIDDKIIRRAQENGETIDALTARFIEAMH
                     EDADALGVLRPDHEPKATETIDGIIAMVGTLVGKDYAYVAANGDVYYRVARFGAYGRL
                     ANKKLDDLRAGERVAVGELKQDPLDFVLWKAAKPGEPAWDSPWGPGRPGWHIECSAMS
                     KCCLGEHFDIHGGGMDLQFPHHENEIAQSEGASGGRFVNVWMHNGFLNVDNEKMSKSL
                     GNFFTIRDVLKAFDGETLRFFMLRTHYRSPFNFSDANLEDARQALRRLYTALDAVLGD
                     ESAAADEAADWSDARA"
BASE COUNT          922 a         2454 c         2510 g         1132 t
ORIGIN      
        1 ggcctggtgg gtgatgccgc tgttcggcgc cgccctgctg gtcggcgcgc tgccgttcgt
       61 gccggcgctg atcgtctcca gcctgcgctt caactaccgg gtgacgcgct ggcgcaacgt
      121 cgccttccgc ttcaccggca gctacggcgg cgcggccgtg gccttcctgc tgtggccgct
      181 gctcggcgtc gtgagtttcg gcctgctgtg gccgaaggcc atccaggcgc agcgcagcta
      241 tctgctgaat cacgtgcact acggcaacgc cgcgttttcg gccgacatcc cgctgtggcc
      301 ggtctacggc tgctcgatcg tcgccagcgt gctgctcggc atcgtcaccg cggtggcgac
      361 ggtgctgagc gggctgatgc tggcgcgcgc cggcctgcgg ccgcagtcca tggcggatct
      421 ggaccactgg cagctttttg cactggtgct gccgacgctc gcctacctgt tcgggctgac
      481 gctgtccgcg gcctattacc gcgcggcact gcgcaacatc gtgctcgccg acgtggcgat
      541 cggcgacggc cacggcctgc gcagccatgt gtcggtgctc gactacatcg ccttcgcgac
      601 cggcaacctg ctgctgaccg tcgtcacgct cgggctggcg cggccgtggg tgcgcgtgcg
      661 caccgcacgc tatttcgcaa ccacgaccac gatccgtttc gccgtgccgg ccggcgcggc
      721 catggccggc atcgccgcgg tgaccgaggc ggccggtcgt cgcggctcga cgctcggcga
      781 tgccgccggc gatgccttcg actgggatct gtcgctgtga gtgaagccgg gagcctgcgc
      841 atcgccggct gccactaccc ggccggcagc gccgccggcc aggccgccga actgcacgtc
      901 agcgccgatg gcgcgctgcg gctcgaaccc ggcggtcgtg cgctcggtca ctggcgcggc
      961 ctgcgcgtgc ccgaacggct cgggcgcgca ccgcgcgtgc tcggactcgg ggatggcggc
     1021 ggcttcgaga ccggcgacaa tgccgccgtc gatgcctgcg aacggcgctt ccgcggccgg
     1081 gtcgccggtg gccggctgca tgcgctcgaa ggccgctgga cgatcgcgtg gctcgcgctc
     1141 gccggactcg gcttctcgat cttcgccggc ttcgtctggg gcatcccggc gctcgcggcg
     1201 cgcgtggcgc cgtggctgcc ggcgtcggtc gtcgtcggca gttccgagga gaccctcgcg
     1261 agtctcgatc gccacgtgct caggccgagc cgcctgccgg cggcgcgctg cgaggcgctc
     1321 gaagccacgc gcgcgcgcct tgccgcgcgt gccggtctga gcggcggccg gctcgaatgc
     1381 cggcgcggcg gtgcgttcgg tgccaatgcc tttgcgctgc cctcggggct ggtcgtgatc
     1441 accgatgagt tcgtcgaact cggcggggac gaggacgagc tggccggcgt gctcgcgcac
     1501 gaggtcgcgc acgtcgagta ccggcacggc ctgcgccagg cgctgcaggg cagcctgctg
     1561 acgctcggtg cggcgctgct gaccggggat cttgccaatg ccggcagctt catcgcgcat
     1621 ctgccggtcg tgctcgccgg cctgcattac tcgcgcgact tcgagcgcga ggccgatgcg
     1681 cgcggtgcgg cgctgatgcg cgccgaaggc cgcgatccgc gcgcgctcgc ccgggcgctg
     1741 gcgcggctcg aaggcgcggc ccggcagaag gaacgtccgg cagcgccggc ctggctgtcc
     1801 gatcacccgg acacgcagga gcgcatcgcg cgcatcgagg cgctggcacg ctgaagcctg
     1861 tgccgcaccg gccgcgaggg cgccggcggg caggctccgg catgagtccg gcttgcccgg
     1921 gcgcgtgcag ccgctacggt ttggatgaat cttcccctgg ccgccccccc ggagcctgcc
     1981 cgatgcccct gcccgtcacg ccgcgcctga ccgtcgatgc catcatcgaa ctcgccgacc
     2041 gccccggccg gccgatcgtg ctgatcgagc ggcgcaaccc gccgccgggc tgggcgctgc
     2101 ccggcggctt cgtcgatgtc ggcgagcgcg tcgaggacgc cgcgatccgc gaggcggccg
     2161 aggagaccgg gctggacgtg cgcctgatca cgctgctcgg catctactcg gacccggcgc
     2221 gcgacgcgcg cttccacacc gccggagtcg tctatgtggc cgaggccacc ggcacgccgg
     2281 tggctgccga tgacgcgcgc cacctcgatg tcttcgatcc ggacggcccg ctgccgctgc
     2341 tcgccttcga tcatgcgcag atcctcgccg attaccgcca gtggcgggca cacggcaccg
     2401 gcgcgccgct gcgcccggcg cactgaattc ccgttctcac ggcagcggat gccatcctga
     2461 tttcatcctg attccgtccg gattccattc tgactgcatc cgttcgccgg tccggcccgc
     2521 atggcttgct gacggcgatg ctgcgcgacc accgctgccg cccgaatgca cgggtgggca
     2581 gcagggcggt ggcgcctgat cacacgggga gtctccatgt ctgcacccgc cgaattgacc
     2641 gatctgctgc tggccagcgg ccgtggcgat cgacctgcct tcgagcgcct gtaccggctc
     2701 agctcgccgc gtctgtactc gctggcgcgc tggctgctca agcgcgatgc gctggccgag
     2761 gagatcatgc aggaggccta cgtgcagatc tggcgcgacg ccgcccgctt cgatccggcg
     2821 cgcgcgtcgg cgatgacctg gatggcggtg atcgtgcgcc accgcgcgct cgacgaactg
     2881 cgccgcaagc gtccggagct cgaacccggc ggtggttccg ccggcagcga cgaggacgat
     2941 gccatgcccg aaccgggagt ggccgaggcc gatccggatg ccggcccgct ggaactcgcg
     3001 ctcgccgacg gcgaccgccg ccagctcggc atctgtctgg agacgctgag cgagatgcag
     3061 cgccagagca tcctgctcgc cttcctgcgc ggcctcacgc actccgaggt cgccagcgcg
     3121 ctggccgcgc cgctcggcag cgtcaagagc tgggtgcgcc gcggactcga acaactgaag
     3181 aggtgcctca cgtcatgaac taccgcgatc cgaaactggg cacccggctc gcggcggagt
     3241 acgcgctggg cacgatggga gggctggccc gccgccgttt cgagggtttg atggccgccg
     3301 atccctggct gcggcgcgag gtgcgcgcct ggcaggacga tctctacggc atcgtcgagc
     3361 tgatcccgga gcgcaccccg cccgagcggc tgtggacgca catcgaaacc cggctgtggc
     3421 cggttgcgac cggcagggtc gtgccgctcg cgccgaaggc ccggccgtcg cggctctggc
     3481 aggtctggag cggactggcg acggcggcgg cattgctgct ggccgtggcc gtgtggcggg
     3541 cgccggcgcc gccctcgccg cagccgctgg cggtgcccga ttacgtggcc gtggtcagtg
     3601 accgcgacgg ccggccggcc tggctggtgc gcccggcgcc gggcggcgga cttgcggtca
     3661 gcgcgctggc gccgcaggcg ctggccagcg accgcgcctt cgaactctgg ctgctgcccc
     3721 ccggcggtgc gccgccgaag tcgctcggcc tgctgccggc cgatggcggc gcgcgcctcg
     3781 gcgtggcgcc ggagcggctg cgcgagatcg tcgagggcga cggcctcgcc gtcagcctgg
     3841 agccggccgg cggctcgccg accggtgccc cgaccggccc ggtgctgtac ctcggcaagc
     3901 cgctgccgcg cgtctgacgc gctcgtgcgc gcctgcgcga cccggcgggg cgcgcaggcg
     3961 gggcagggcg ccgaggccgg atttcagccg tgcaggcctt cgagctgcat gccgttgcgc
     4021 gagaccgcca gcacgctgcc ctgcgtgtac cagtcaccga gcacgacgcg ccagccgctg
     4081 tcgccatcgg cgagcacgag ttcgtgcagg gcggggcggt gggtgtggcc gtggatcagc
     4141 tgggtgacgc cgtgtgcgtc catgagcgtg cgcacggcat cggggtttac gtcggtgatc
     4201 tcggccggct tgtcggcctg ggcgctgcgg ctgtcggcgc gcagctgcgc ggcgaaggcc
     4261 cggcgctgcg cgagcggcag cgccagcagg cgagcctgat tggccggttc gcggatctgt
     4321 gcgcggaagc gctggtaatc gacatcgtcg gtgcagagcg catcgccgtg cgtgagcagc
     4381 gtcggcgtgc cgtacaggtc gatcaccgtg gtctccggca gcagggtcag tccggcgcgt
     4441 gccgcatagt ccgggccgag caggaaatcg cggttgccgt gcaggaagtg cgcgggcacg
     4501 ccgctgcgct gcagactgcc cagcgccgcg gccacccgct cgccggtttc gccgtcgtcg
     4561 tcgtcgccga tccaggcctc gaacaggtcg ccgaggatgt acagcgcctg cgcgcgccgg
     4621 gcgcggacgg cgatgaaatc ctcgaacagc gccagcgtct gcgggcgcgc ggcagcatcg
     4681 aggtgcaggt cggcgacgaa cagcgtcgcc ttcattcgac gatttccatc ttctcgatca
     4741 gcaccggctc gaccggcacg ttctggtgtc cgcgcttgtt cgcggtcggc acggcgacga
     4801 tcgcatcgac cacgtccatg ccctcgatga ccttgccgaa cacgcagtac ccccagccgt
     4861 cctgccccgg gtgatcgagg aagccgttgt ccttgacgtt gatgaagaac tgcgcggtcg
     4921 ccgagtgcgg atccgaggtg cgcgccatcg cgatgctgcc gcgggcgttc ttcaggccgt
     4981 tcttcgcctc gttgcggatc ggcgcgcgcg tgctcttctg gctcatgtcc gccagcatgc
     5041 cgccgccctg gaccatgaag ttcgggatca cgcgatggaa gacggtgccg gcgtagaaac
     5101 cgtcgcgcac gtactgttcg aagttggcgg tggttgccgg cgcatgctcg gcgtcgagtt
     5161 ccagcgtgat gacgccgagt gaggtctgga tgcggatcat cgcgaatctc cgttcggggt
     5221 ttccgggggc gggcctgctt tatctcatga ctacttcacg accgtgaccg agcggatcac
     5281 caccggctcg gccggcacgt cgctgtatgg tccgaccgtg gtggtcggtg tctggcggat
     5341 gcgctcgacg acttcgaggc cgtcgacgac cttgccgaac acgcagtaac cccagccgtc
     5401 acgcgacggg taatcgagga agccgttgtc cctgacgttg atgaagaact gcgcggtcgc
     5461 cgagtgcgga tcggcggtgc gtgccatcgc gatcgtgcca cggctgttct tcaggccgtt
     5521 ctttgcttcg ttcgggatcg ggtcgtgcgt cggcttctgt tgcaggtcgg gcgtgaagcc
     5581 gccgccctgg atcatgaagt tgtcgatgac gcggtggaag atcgtgccgt cgtagaagtg
     5641 ctcgtccgcg tagcgcacga agttggcgac ggtctgcggg gctttttcct cgtcgagttc
     5701 gagcacgatc gtgccgtgac tggtgaccat ctgcacgcgg gtcaccgcca tggccggcag
     5761 ggcggccgaa agcagggcag cgatcaggaa ggctcgggcg caacggatca tcggcagcct
     5821 ccgggggcgg gcgcggaaag gccggcatca tagtgtgccg cccggctttt gtcggcctgc
     5881 acgcgccgga cttccgttac catcccggtc gggccctgct gccggggcgc cgtccgtccg
     5941 ttttccgtcg catcgagtcc agtccgtcat gtccgtcgaa cacgatctgc agctgtacaa
     6001 cagcctgagc cggcgcaagg agcgcttcgt cccgatcacg cccggccagg tgcgcatgta
     6061 tgtgtgcggc atgaccgtct acgactactg ccacgtcggc cacgcgcgcg tgatggtcgt
     6121 gttcgatgcg atcgtgcgct acctgcgctg gctcggctac gacgtgacct acgtccgcaa
     6181 catcaccgac atcgacgaca agatcatccg ccgcgcgcag gaaaacggcg aaaccatcga
     6241 cgcgctgacc gcgcgcttca tcgaggcgat gcacgaggat gccgatgcgc tcggcgtgct
     6301 gcgccccgac cacgagccga aggccaccga aaccatcgac ggcatcatcg cgatggtcgg
     6361 gacgctggtc ggaaaggact acgcctacgt cgctgccaac ggcgatgtct actaccgcgt
     6421 ggcgcgcttc ggggcctacg gccggctcgc caacaagaag ctcgacgacc tgcgcgccgg
     6481 cgagcgcgtg gcggtcggcg aactgaagca ggacccgctc gacttcgtgc tgtggaaggc
     6541 cgccaagccc ggcgagccgg cctgggattc gccgtgggga ccgggccggc cgggctggca
     6601 catcgaatgc tcggccatgt cgaagtgctg cctcggcgag cacttcgaca tccacggcgg
     6661 cggcatggac ctgcagttcc cgcaccacga gaacgagatc gcgcagagcg agggcgccag
     6721 cggcgggcgc ttcgtcaacg tctggatgca caacggcttc ctcaacgtcg acaacgagaa
     6781 gatgtcgaag tcgctcggca acttcttcac catccgcgac gtgctgaagg ccttcgacgg
     6841 cgagacgctg cgcttcttca tgctgcgcac gcactaccgc agtcccttca acttcagcga
     6901 cgccaatctc gaggatgcgc gccaggcgct gcgccggctc tacacggcgc tcgacgccgt
     6961 gctcggtgac gaatctgcag cggcagacga ggcggcggac tggagcgacg cgcgcgcc
//
LOCUS       DAOQJH010000030         6855 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_6160_length_6855_cov_3.848088, whole genome shotgun sequence.
ACCESSION   DAOQJH010000030 DAOQJH010000000
VERSION     DAOQJH010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 6855)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6855)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6855
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6160_length_6855_cov_3.848088"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..993
                     /gene="sufB"
                     /locus_tag="PLQ18_01360"
     CDS             <1..993
                     /gene="sufB"
                     /locus_tag="PLQ18_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006162013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HNE00325.1"
                     /translation="CSFSEAVREHPELVRQYLGTVVPVRDNYYAALNSAVFSDGSFVY
                     IPKGVRCPMELSTYFRINAKNTGQFERTLIVADAGAYVSYLEGCTAPMRDENQLHAAV
                     VELVAIDDASIKYSTVQNWYPGDKEGRGGIYNFVTKRGLCRGARSKISWTQVETGSAI
                     TWKYPSCILRGDDSVGEFYSVALTRDRMQADTGTKMIHMGKRTRSTIVSKGISAGHAQ
                     NAYRGLVKILPTADGARNHSQCDSLLMGDQCGAHTFPYVEVQNPTAQVEHEATTSRVS
                     DDQIFYLNQRGIGTEDAVSLIVNGFCKEVFKELPMEFAVEAQKLLELKLEGSVG"
     gene            1080..1850
                     /gene="sufC"
                     /locus_tag="PLQ18_01365"
     CDS             1080..1850
                     /gene="sufC"
                     /locus_tag="PLQ18_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014249538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HNE00326.1"
                     /translation="MLEIRNLHAALEGREILKGIDLSIRAGEVHAIMGPNGSGKSTLA
                     NVLAGRPGYEASAGEVLFDGQDLLALAAEERAREGLFLAFQYPVEIPGVNNLYFLKAS
                     INAIRKHRGQEELDALEFLERVQEKAGLVKMDEAFLKRPVNEGFSGGEKKRNEILQMA
                     VLEPKFAVLDETDSGLDIDALKVVADGVNALRSPERGFLVITHYQRLLDYIVPDVVHV
                     LVDGRIVRSGGPELAHELEAQGYTLYGREAGASRRAQA"
     gene            1874..3169
                     /gene="sufD"
                     /locus_tag="PLQ18_01370"
     CDS             1874..3169
                     /gene="sufD"
                     /locus_tag="PLQ18_01370"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HNE00327.1"
                     /translation="MNPVAVTAVTDPRAHWLAAATETAAGLPPELALPELRAAARTRF
                     ERLGLPSRTLEAWRTTDIAPLRGLEPRRADTGRAPDAGWLAGLPAGPRVVLVDGRVHA
                     IETGELPAGVSVLGFAEAARRLPQVLAALDTLPGLDAHAFAALNSALFEDGVLVHVAA
                     GARLDALTLAHWSSGDGTAVHPRLLLVLEDGAGAGLLEWQGGQGTYLSTALTEVRLGR
                     GAQLALSTVQDESAEAFQLGGLRAQLAADAKFSLRRWVGGGRIGRMDLALTLEGEGAA
                     AELDGLVLAGGTQLLDHHLELRHAVARCTSRQTFRGVLAGKAKHVFDGLIRVDPDAQQ
                     TDAQQQCRNLLLSKQALTVAIPRLEILADDVKCAHGATVGALDEDALFYLRARGIGLA
                     EARTLLVRAFAGEFLDALTDEAMRAALETRLDAWMEVAA"
     gene            3166..4419
                     /locus_tag="PLQ18_01375"
     CDS             3166..4419
                     /locus_tag="PLQ18_01375"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HNE00328.1"
                     /translation="MSAPIVTPVPAAAFDVEAVRAQFPVLAQQIRGKHLVYLDSGASA
                     QKPEVVVQAVADCYRGYYSNIHRGVHALSERSTTAFERAREQVARFVNAPSPREIVFL
                     RSTTEAINLVANSFLEPQLGPDDEILVTEMEHHSNIVPWQLVADRYGAKVVAAPVTDR
                     GELDLDGLTRLISGRTKLVAVTHVSNVLGTINPIADIVRLAHAQGVPVLVDGAQGVPH
                     LAVDVQALGCDFYAFSAHKVYGPSGVGALWARAELLAAMPPYQGGGGMIRRVSFSGTT
                     FADPPERFEAGTPDVAGVIGFVAAVEWTRALGLDAIAAHEHELTQYAMARLADIPGLR
                     LIGTAADKAGVVSFVMDGVHPHDIGTILDGEGVAVRVGHHCAQPLMERFAVPATARAS
                     FGVYNTSADVDALAAALDKVRHWFG"
     gene            4463..4906
                     /locus_tag="PLQ18_01380"
     CDS             4463..4906
                     /locus_tag="PLQ18_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009770556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HNE00329.1"
                     /translation="MSDLRELYQEVILDHNKRPRNFRVIDPASHHAEGFNPLCGDRLT
                     VFIDVADGVIRDLAFQGQGCAISTASASLMTEALKGRPVAEVEPLFVKFHDLVTTDGP
                     TPPGLGKLGVLAGVREFPSRVKCATLAWHALKAALDHRSAPVATE"
     gene            4924..5274
                     /locus_tag="PLQ18_01385"
     CDS             4924..5274
                     /locus_tag="PLQ18_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system Fe-S cluster assembly protein"
                     /protein_id="HNE00330.1"
                     /translation="MNDAAQPSADVTPEQVEVEQQIVRALKTVYDPEIPVDIWELGLV
                     YGVNVNLGDRSAHIYMTLTAPACPVAGSMPEWVENAVRGVDGIDDVTVELVWDPPWTQ
                     EMISARARLELNLL"
     gene            5422..>6855
                     /locus_tag="PLQ18_01390"
     CDS             5422..>6855
                     /locus_tag="PLQ18_01390"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing methyl-accepting
                     chemotaxis protein"
                     /protein_id="HNE00331.1"
                     /translation="MQPTHLLGDRLRRTGVYAVLQALVLMAVALSAVTFLALQLQAAR
                     TAAQALRNHLEADMMHDALRGDVLMARTPGFTPAEVQEELRDHATRFRDLLAANRSLD
                     VPKVLADAFAAVAPQAERYIASAEQEVAAVLGGQSGDLPGFITQFNHLEDAMERVSEA
                     VSANAADTEAAGNRAALLAAGLLLLVGIGTLSAALLGGRRLARDVSMPLLRLADGMRR
                     LAAGERGLDRADCARPDEIGEMARALLVFEETAVRAEALANARAAEHAAVEAERERVR
                     LADEQARLAAEAEREQRRQVEAEAREQRQRERERRAERVAQLAADFERQSNSSLQAVT
                     NAAQDLTRTAQGLAGGAERTQQRAEAVGKASSGAADSVRRMAEASAQMAVAVSQIAAQ
                     AGQSAQTTRLAVDEAAATQRTVSGLSDAARTIGRIVDLINTIADQTNLLALNATIESA
                     RAGEAGKGFAVVANEVKALAHQTAEATD"
BASE COUNT         1054 a         2437 c         2391 g          973 t
ORIGIN      
        1 tgctcgttct cggaggccgt gcgcgagcat ccggaactcg tgcgccagta cctcggcacg
       61 gtcgtgccgg tgcgtgacaa ctactacgcg gcgctgaact cggcggtgtt ctcggacggc
      121 tccttcgtct acatcccgaa gggcgtgcgc tgcccgatgg agctgtcgac ctacttccgc
      181 atcaacgcca agaacaccgg ccagttcgag cgcacgctga tcgtcgccga tgccggcgcc
      241 tacgtgagct acctggaagg ctgcacggcg ccgatgcgcg acgagaacca gctgcatgcg
      301 gcggtcgtcg agctggtggc gatcgacgac gcgtcgatca agtactcgac cgtgcagaac
      361 tggtatccgg gcgacaagga aggccgcggc ggcatctaca acttcgtgac caagcgcggc
      421 ctgtgcaggg gcgcgcgctc gaagatttcc tggacgcagg tcgagaccgg ctcggcgatc
      481 acctggaagt acccgagctg catcctgcgc ggtgacgatt cggtcggcga gttctactcg
      541 gtggcgctga cgcgcgaccg catgcaggcc gacaccggca ccaagatgat ccacatgggc
      601 aagcgcacgc gctcgaccat cgtctccaag ggcatctcgg ccggtcatgc gcagaacgcc
      661 taccgcggcc tcgtgaagat cctcccgacc gccgacggcg cccgcaacca ttcgcagtgc
      721 gactcgctgc tgatgggcga ccagtgcggc gcgcacacct tcccgtacgt cgaggtgcag
      781 aacccgacgg cccaggtcga gcacgaggcg accacctcgc gcgtctccga cgaccagatc
      841 ttctacctga accagcgcgg catcggcacc gaggacgcgg tcagcctgat cgtcaacggc
      901 ttctgcaagg aagtgttcaa ggagctgccg atggagttcg ccgtcgaggc gcagaagctg
      961 ctcgaactga agctcgaagg cagcgtcggc tgatcgccgt cgcgcgcccg cacccgattc
     1021 ctcctctact gacccgatcg accggccctg cgccggctcc ccggaaaccg gaacgaacca
     1081 tgctggaaat ccgcaatctg cacgccgcgc tggaaggccg cgaaatcctc aagggcatcg
     1141 acctgagcat ccgcgccggc gaagtccacg cgatcatggg cccgaacggc tcgggcaaga
     1201 gcacgctcgc caatgtgctg gccgggcggc ccggctacga ggccagtgcc ggcgaggtgc
     1261 tgttcgacgg tcaggacctg ctggcgctgg ccgccgagga gcgcgcacgc gaggggctgt
     1321 tcctcgcctt ccagtacccg gtcgagattc cgggcgtcaa caacctgtac ttcctgaagg
     1381 cttcgatcaa cgccatccgc aagcaccgcg gccaggagga gctcgacgcg ctcgaattcc
     1441 tcgaacgcgt gcaggagaag gccgggctcg tgaagatgga cgaagccttc ctgaagcgcc
     1501 cggtcaacga aggcttctcg ggcggcgaga agaagcgcaa cgaaatcctg cagatggcgg
     1561 tgctggagcc gaagttcgcg gtgctcgacg agaccgactc cggcctcgac atcgacgcgc
     1621 tgaaggtcgt cgccgatggc gtcaacgcgc tgcgcagccc cgaacgcggc ttcctggtga
     1681 tcacgcacta ccagcgcctg ctcgactaca tcgtgcccga tgtcgtgcac gtgctggtcg
     1741 atggccgcat cgtgcgctcc ggcgggccgg agcttgcgca cgaactcgaa gcccagggct
     1801 acacgctgta cggccgcgaa gccggcgcca gtcgccgggc gcaggcctga gcggggtggg
     1861 cggcgtgagt accgtgaatc ccgtagccgt cactgccgtg accgatccgc gcgcgcactg
     1921 gctggccgcg gcgaccgaga ccgccgccgg cctgccgccg gagctggcgc tgcccgaact
     1981 gcgcgccgcg gcgcgcacgc gcttcgagcg tctcggcctg ccgtcgcgca cgctggaggc
     2041 ctggcgcacg accgacatcg cgccgctgcg cgggctcgaa ccgcggcgtg ccgacaccgg
     2101 gcgtgcgccc gacgccggct ggctggccgg gctgccggcc gggccgcgcg tcgtgctggt
     2161 cgatggccgg gtgcacgcga tcgaaaccgg tgaactgccg gcaggcgtca gcgtgctcgg
     2221 cttcgccgag gccgctcgcc ggctgccgca ggtgctggcc gcactcgaca cgctgccggg
     2281 cctcgatgcg catgccttcg ccgcgctcaa ctcggcgctg ttcgaggacg gcgtgctcgt
     2341 gcacgtcgcc gccggtgcgc ggctcgacgc gctgacgctc gcgcactgga gcagcggtga
     2401 cggcacggcc gtgcatccgc gcctgctgct ggtgctcgaa gacggagccg gggccgggct
     2461 gctggagtgg cagggcgggc aggggaccta tctgtcgacg gcgctgaccg aggtccggct
     2521 cggacgcggc gcgcagctgg cgctgagcac ggtgcaggat gaatccgccg aggccttcca
     2581 gctcggcggc ctgcgcgcgc agctcgccgc cgacgcgaag ttttcgctgc gccgctgggt
     2641 cggcggcggg cgcatcggcc ggatggatct cgcgctcacg ctcgaaggcg agggggccgc
     2701 ggccgaactc gacggtctgg tgctggccgg cggcacgcag ctgctcgatc accacctcga
     2761 actgcgtcat gccgtggcgc gctgcacgag tcgccagacc ttccgcggcg tgctcgccgg
     2821 caaggcgaag cacgtgttcg acggcctgat ccgcgtcgat ccggatgcgc agcagaccga
     2881 cgcgcagcag cagtgccgca acctgctgct gtcgaagcag gcgctgacgg ttgcgatccc
     2941 gcgcctcgaa atcctcgccg acgacgtgaa gtgcgcgcac ggcgcgacgg tcggcgcgct
     3001 cgatgaggat gcgctgttct acctgcgcgc gcgcggcatc ggtctggccg aggcgcgcac
     3061 gctgctggtg cgcgccttcg ccggtgagtt cctcgacgcg ctgaccgacg aggccatgcg
     3121 cgccgcgctc gaaacccgac tcgatgcctg gatggaggtg gccgcatgag cgccccgatc
     3181 gtgaccccag tacccgcagc ggccttcgat gtcgaggccg tgcgcgcgca gttcccggtg
     3241 ctcgcacagc agatccgcgg caagcatctc gtatacctcg acagcggcgc ctcggcgcag
     3301 aagcccgagg tcgtcgtgca ggcggtcgcc gactgctacc gcggctacta ctcgaacatc
     3361 caccgcggcg tgcatgcgct gtccgagcgc tcgaccaccg ccttcgagcg cgcgcgcgag
     3421 caggtcgcac gcttcgtcaa cgcgccgtcg ccgcgcgaga tcgtgttcct gcgcagcacg
     3481 accgaggcga tcaacctggt cgcgaacagt tttctggagc cgcagctcgg gcccgatgac
     3541 gagatcctcg tcaccgagat ggagcaccac tccaacatcg tgccctggca gctggtggcc
     3601 gaccgctatg gcgcgaaggt cgtcgccgct ccggtcaccg accgcggcga gctcgacctc
     3661 gacggactga cgcggctgat cagcggccgc acgaagctcg ttgcggtcac gcacgtctcg
     3721 aacgtgctcg gcacgatcaa cccgatcgcc gacatcgtgc gtctggcgca cgcgcagggc
     3781 gtgccggtgc tggtcgacgg cgcgcagggc gtgccgcatc tggccgtcga cgtgcaggcc
     3841 ctcggctgcg acttctatgc gttctcggcg cacaaggtct acgggccgag cggcgtcggc
     3901 gcgctgtggg cgcgtgccga gctgctggcg gcgatgccac cgtatcaggg cggcggcggc
     3961 atgatccggc gcgtgagctt ctcgggcacg accttcgccg atccgccgga gcgcttcgag
     4021 gccggcacgc cggatgtcgc cggcgtcatc ggcttcgtcg ccgcggtcga gtggacgcgc
     4081 gcgctcgggc tcgatgccat cgccgcgcac gagcacgaac tgacgcagta cgcgatggcg
     4141 cgtcttgccg acatcccggg cctgcgcctg atcggcaccg cagcggacaa ggccggggtc
     4201 gtgtccttcg tgatggacgg cgtgcatccg cacgacatcg gcacgatcct cgacggcgaa
     4261 ggcgtcgccg tgcgcgtcgg tcaccactgc gcgcagccgc tgatggagcg cttcgcggtg
     4321 ccggcgaccg cccgcgcctc gttcggcgtc tacaacacgt ccgccgatgt cgacgcgctc
     4381 gccgccgcgc tcgacaaggt ccgccactgg ttcggctgaa acaccccgga cctcaggaac
     4441 cgaattcctt caaggtgttg cgatgagtga cctgcgcgag ctgtaccagg aagtgatcct
     4501 cgatcacaac aagcgccccc ggaacttccg cgtgatcgac ccggcctcgc atcacgccga
     4561 gggcttcaac ccgctctgcg gcgaccggct gaccgtgttc atcgacgtgg ccgacggcgt
     4621 gatccgcgac ctcgccttcc agggccaggg ctgcgcgatc tcgaccgcgt cggcctcgct
     4681 gatgaccgag gccctgaagg gcaggccggt cgccgaggtc gagccgctgt tcgtgaagtt
     4741 tcatgatctc gtcaccaccg acggcccgac gccacccggc ctcggcaagc tcggcgtgct
     4801 cgccggcgtg cgcgagttcc cgagccgcgt caagtgcgcg acgctcgcct ggcacgcatt
     4861 gaaggccgcg ctcgatcacc gcagcgcgcc ggtggcgacc gaatgaagcc cggagagtcc
     4921 gcgatgaacg atgccgccca gccatccgca gacgtgaccc ccgaacaggt cgaggtcgaa
     4981 cagcagatcg tcagggcgct gaagaccgtc tacgaccccg agatcccggt cgacatctgg
     5041 gagctcggcc tcgtctacgg cgtcaacgtc aacctcggcg atcgcagcgc gcacatctac
     5101 atgacgctga ccgccccggc ctgcccggtg gccggctcga tgcccgagtg ggtcgagaac
     5161 gccgtgcgcg gcgtcgacgg catcgacgac gtgaccgtcg agctggtctg ggacccgccg
     5221 tggacgcagg agatgatctc ggcgcgggca cggctggagc tgaacctgct ctagccgcag
     5281 gccgctgccg gcgccgcgtg accggctcgg cggcggctgc gcaccgtgca gcgccgtcgc
     5341 gtctgccggc ttcagtcttg ccgattcctg ccgatgcctg tcgggcatac gaggctcgtc
     5401 cacggaattg cggagactcc gatgcagccc acccacttgc tcggcgatcg cctgcgcagg
     5461 accggcgtct atgccgtgct gcaggcgctc gtgctgatgg ccgtcgcgtt gtccgcggtc
     5521 accttccttg cgctgcagtt gcaggcggca cgcaccgccg cgcaggcgct gcgcaaccac
     5581 ctcgaagccg acatgatgca cgacgcgctg cgcggcgacg tgctgatggc gcgcacgccg
     5641 gggttcacgc cggccgaggt gcaggaggaa ctgcgcgatc acgccacgcg tttccgggac
     5701 ctgctggccg ccaaccgcag cctcgatgtg ccgaaggtgc tggccgacgc gttcgctgcc
     5761 gtcgcgccgc aggccgagcg ctacatcgcc agcgccgagc aggaggtcgc cgccgtgctc
     5821 ggcggccagt ccggcgatct gccgggattc atcacgcagt tcaaccacct tgaagatgcg
     5881 atggagcgcg tcagcgaggc ggtcagcgcc aatgccgccg acaccgaagc cgccggcaac
     5941 cgggcagccc tgctcgcggc cggcctgctg ctgctggtcg ggatcggcac gctcagtgcc
     6001 gccctgctcg gcgggcggcg gctggcacgt gacgtgagca tgccgctgct gcgcctggcc
     6061 gacggcatgc gccggctcgc tgccggcgag cgcgggcttg atcgcgcgga ctgcgcgcgc
     6121 cccgatgaaa tcggcgaaat ggcgcgggcg ctgctggtgt tcgaggaaac cgcggtgcgc
     6181 gccgaggcgc tggccaatgc ccgggcggcc gaacatgcgg cggtcgaggc cgagcgcgag
     6241 cgcgtgcgcc tcgccgatga gcaggcgcgc ctggctgccg aagccgagcg cgagcagcgc
     6301 cgccaggtcg aggccgaagc ccgcgaacag cgccagcgcg agcgcgagcg gcgggccgag
     6361 cgcgtcgcgc agctggccgc ggacttcgag cgccagtcga acagcagcct gcaggcggtc
     6421 acgaacgcgg cccaggacct gacccgcacc gcgcaggggc tggccggcgg cgccgaacgc
     6481 acgcagcagc gcgccgaagc ggtcggcaag gcttcctcgg gggcggccga cagcgtccgg
     6541 cgcatggccg aggccagtgc gcagatggcg gtcgccgtca gccagatcgc cgcgcaggcc
     6601 ggacagtccg cccagaccac acgcctcgcc gtcgacgagg ccgccgcaac ccagcgtacg
     6661 gtttccgggc tcagtgacgc ggcgcgcacg atcggccgca tcgtcgacct gatcaacacg
     6721 atcgccgacc agaccaatct gctggcgctg aacgcgacga tcgaatcggc ccgtgccggc
     6781 gaggccggca agggctttgc ggtggtcgcc aacgaggtca aggcgctcgc ccaccagacc
     6841 gccgaagcca ccgac
//
LOCUS       DAOQJH010000031         6794 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_6228_length_6794_cov_4.243953, whole genome shotgun sequence.
ACCESSION   DAOQJH010000031 DAOQJH010000000
VERSION     DAOQJH010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 6794)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6794)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6794
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6228_length_6794_cov_4.243953"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..473)
                     /locus_tag="PLQ18_01395"
     CDS             complement(<1..473)
                     /locus_tag="PLQ18_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019997866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1285 domain-containing protein"
                     /protein_id="HNE00332.1"
                     /translation="MPDSDPSRLLAQAGPRRGRPPLERWQPPLSGDIDIRIDRDGRWW
                     HEGRPIEREALVQLFAGILRREADGGHYLVTPIEKWRITVEDAPLLAGPPERLDGPAG
                     PRLRFTTSVGDVFDAGPQYPIEVEYRGDEPRPYLHVHHGLRARLTLGYVDALAALP"
     gene            581..859
                     /locus_tag="PLQ18_01400"
     CDS             581..859
                     /locus_tag="PLQ18_01400"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010628086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNE00333.1"
                     /translation="MAKARARHILVDSEAACLDIKRQIEEGADFAEMARLHSSCPSKR
                     QGGDLGEFGPGMMVREFDEAVFSGEVGKVLGPVRTQFGWHLLEVTQRS"
     gene            865..1263
                     /locus_tag="PLQ18_01405"
     CDS             865..1263
                     /locus_tag="PLQ18_01405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE00334.1"
                     /translation="MRASRLLLSAALAAALAGCTGLSLPAGKSSPDPVGLAKLDGLWR
                     GAGKADGLTVRIARAGSSQPRMMYGFRDEAPTQPQCTVRAPATLSCKHESGLTTTYTQ
                     SGDSTLDFTAQGPKEGDEVLRAQLSRMREP"
     gene            complement(1354..1893)
                     /locus_tag="PLQ18_01410"
     CDS             complement(1354..1893)
                     /locus_tag="PLQ18_01410"
                     /inference="COORDINATES: protein motif:HMM:NF017559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HNE00335.1"
                     /translation="MSAVFNSPPAETRWYRQFWPWVLLGLPASAVTGCLITIWIATSN
                     PDGVVVDDYYKQGLAINTDQGRSVRASALGLHGRLQLDADSGRIGVDLAADAAGAQLP
                     PALTLRLLHATRSGIDVSLTLAGVAPGRYEAALPALAPGRWNLKAEAGDWRLDGSLNA
                     PHENAVALLPLVQMLPAER"
     gene            complement(1913..3322)
                     /gene="ccoG"
                     /locus_tag="PLQ18_01415"
     CDS             complement(1913..3322)
                     /gene="ccoG"
                     /locus_tag="PLQ18_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002689920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HNE00336.1"
                     /translation="MTAETPSRPEGQDSGKSSFYAKTAKIYAREVKGRFATLRIYAVF
                     ALLGLYYGLPWLPWDGRQAVLFDLPARKFYIFGWVFWPQDFFFLALLLIIAALALFFF
                     TALAGRLWCGYACPQTVWTETFMWIEHWVEGDRNARMKLDKAPWSAGKFRKKLLKHSL
                     WALFALWTGFTFVGFFTPMHDLAARLWPFDWGPWETFWVFFYAFATWGNAGFMREQVC
                     LYMCPYARFQSAMFDKDTLVISYDAERGEPRGSRKRGTDPKAAGLGSCVDCTMCVQVC
                     PTGIDIRNGLQYQCIGCAACIDACDDIMDKMNYPRGLIRYTTEHSLMHDVPHVIRPRM
                     MVYGGLLLALTIGLLVAIALRVPLQLDVIRDRNALYRMNDEGLVENVYTLKIINMDER
                     EHRYRLGVSGVPGLKFTQDPGEIRVGSGEVFDLPTRVSADPEELKAASNEIRFDLVSV
                     DAPKLKTVTAGRFLGPARH"
     gene            complement(3493..3768)
                     /locus_tag="PLQ18_01420"
     CDS             complement(3493..3768)
                     /locus_tag="PLQ18_01420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE00337.1"
                     /translation="MITRVQHIASLLWPSFLLAAAGTVILSTIFDPLAIAHCLGVTGI
                     ERTGTYSLGFFALWMMNTCSALLTLYFRQPVHTPVVCPVRDFDPDQN"
     gene            complement(3995..4882)
                     /gene="ccoP"
                     /locus_tag="PLQ18_01425"
     CDS             complement(3995..4882)
                     /gene="ccoP"
                     /locus_tag="PLQ18_01425"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008567806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit III"
                     /protein_id="HNE00338.1"
                     /translation="MSLTVSLYITLIVVGNTLAMWWLIRWTAKPRPNESVQGEVTGHT
                     WDGDLAEFNNPMPRWWLWMFYLTMVFTGVYVALYPGLGSVTGFLGWTQEGQWQSEMKD
                     AADKYDPIFKKYAGMDIAAVAKDPQALKMGGRLFATYCSSCHGSDAHGAVGFPNLADK
                     DWLYGGDPATIKTSIMNGRQGVMPAWSAILKEQGVEQVANYVLSLSGKSVDAAKAEAG
                     KALFLQNCAVCHGAEGKGNPVMGAPNLADNTWLYGGSLKAVEKTIADGRQGRMPAHAD
                     FLGNDKVHLLAAYVYSLSQ"
     gene            complement(4898..5068)
                     /locus_tag="PLQ18_01430"
     CDS             complement(4898..5068)
                     /locus_tag="PLQ18_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008174730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit 3"
                     /protein_id="HNE00339.1"
                     /translation="MTVVRSVFTVLMFVIFIGIVWWAWSRHNRKRFDDAASLPFADED
                     TARRTVEQEDRR"
     gene            complement(5087..5719)
                     /gene="ccoO"
                     /locus_tag="PLQ18_01435"
     CDS             complement(5087..5719)
                     /gene="ccoO"
                     /locus_tag="PLQ18_01435"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010385442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit II"
                     /protein_id="HNE00340.1"
                     /translation="MAHDNAPAVGHEKVERNIGLMVVLILIVVAFGGLAEIVPLFFLK
                     DTTEPVAGLKPLTALQLEGRDIYIREGCYTCHSQMVRPFRAETERYGHYSVAGEFVYD
                     HPFQWGSKRTGPDLARVGGRYSDDWHRTHLINPRDVVPESNMPGFPWLDTGKLDGELT
                     GKKMQTLRLVGVPYTDADIQGAKAAVQGKTEIEALIAYLQNLGTVISNKR"
     gene            complement(5735..>6794)
                     /gene="ccoN"
                     /locus_tag="PLQ18_01440"
     CDS             complement(5735..>6794)
                     /gene="ccoN"
                     /locus_tag="PLQ18_01440"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018230122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit I"
                     /protein_id="HNE00341.1"
                     /translation="EYAELEWPIDILIAVVWIAYAILFFGTIMKRRTPHIYVANWFYG
                     AFILTVALLHVVNSAELPVSLTKSYSVYPGAIDAMVQWWYGHNAVGFFLTAGFLGMMY
                     YFVPKQAGRPVYSYRLSVVHFWALISLYMWAGPHHLHYTALPNWTQSIGMVFSLMLLA
                     PSWGGMINGIMTLSGAWHKLRTDPILRFMIVSLSFYGMSTFEGPMMSIKTVNALSHYT
                     DWTIGHVHSGALGWVALISIGCVYALIPQLFNRRIYSTKLIEVHFWTGTIGIVLYIAS
                     MWIAGVMQGLMWRAVNEDGTLTYSFVEALVRMYPYLTVRFLGGLLFLVGMFVMAWNVY
                     KTLQGDEQPVSAPAPQAA"
BASE COUNT         1141 a         2245 c         2334 g         1074 t
ORIGIN      
        1 ggcagcgcgg ccagcgcgtc gacgtagccc agcgtcagcc gggcgcgcag gccgtgatgc
       61 acgtgcaggt aggggcgcgg ttcgtcgccg cgatattcga cctcgatcgg gtactgcggg
      121 ccggcatcga agacatcgcc gacgctggtc gtgaagcgca gccgcgggcc ggccggaccg
      181 tcgagccgct cgggcgggcc ggcgagcagc ggcgcatcct cgaccgtgat ccgccacttt
      241 tcgatcggcg tgaccaggta atgcccgccg tcggcctcgc gacgcaggat gccggcgaac
      301 agctgcacca gcgcctcgcg ttcgatcggc cggccctcgt gccaccagcg gccgtcgcgg
      361 tcgatgcgga tgtcgatgtc gccgctgagc ggcggctgcc agcgttcgag cggcgggcgg
      421 ccgcggcggg ggccggcctg cgcgagcagg cgggacggat cgctgtccgg catgtcatat
      481 cctccgcggc cgttgcatcg ccggtcgctt gctgttcaag ccgccggacc gccgctaagc
      541 tcccggcccc gtttcccatg agcagatgga gagattcccc atggccaaag cccgtgcccg
      601 acacatcctc gtcgattccg aggcggcctg cctcgacatc aagcgccaga tcgaggaagg
      661 cgccgacttc gccgagatgg cccgcctgca ttcctcgtgt ccgtcgaagc gccagggcgg
      721 cgacctcggc gagttcggcc ccggcatgat ggtgcgcgaa ttcgacgaag ccgtgttcag
      781 cggcgaggtc ggcaaggtgc tcggcccggt ccggacgcag ttcggctggc acctgctcga
      841 agtcacgcag cggagctgat cgtgatgcgt gcgtcccgtc tgctgctgtc cgccgcgctc
      901 gcggccgccc tcgccggctg caccggcctg tcgctgccgg ccggcaagtc gtcacccgac
      961 ccggtcgggc ttgcgaagct cgacggtctc tggcgcggtg ccggcaaggc cgatggcctc
     1021 accgtgcgca tcgcacgcgc cggttcctcg cagccgcgca tgatgtacgg cttccgcgac
     1081 gaggccccga cgcagccgca atgcacggtg cgcgcgccgg cgacgctgag ctgcaagcac
     1141 gagagcgggc tgacgacgac gtatacccag tccggcgaca gcacgctcga tttcacggcg
     1201 cagggcccga aggaaggcga cgaggtgctg cgcgcgcagc tctcgcgcat gcgcgaaccc
     1261 tgatcccgcg gcgcccccgc cgccagccat cgcgataaaa aagcccgggc atccgcccgg
     1321 gctttttttg tggctgcggc ttctgcggga gcttcagcgc tcggccggca gcatctgcac
     1381 cagcggcagc agcgccacgg cgttctcgtg cggggcgttc aggctgccgt cgagccgcca
     1441 gtccccggct tcggccttca ggttccagcg cccgggcgcg agcgccggca gcgccgcctc
     1501 gtagcgtccc ggcgcgacgc cggccagcgt cagcgacacg tcgatgccgc tgcgcgtcgc
     1561 gtgcagcaga cgcagcgtca gcgccggcgg cagctgcgcg ccggcggcat cggcggcgag
     1621 gtcgacgccg atccggccgc tgtcggcatc gagctgcagg cggccgtgca ggccgagcgc
     1681 cgaggcgcgc acgctgcggc cctgatcggt gttgatcgcc agcccctgct tgtaatagtc
     1741 gtcgacgacg acgccatcgg ggttgctcgt cgcgatccag atcgtgatca ggcagccggt
     1801 gacggcactc gccggcaggc cgagcagcac ccaaggccag aactgccggt accagcgcgt
     1861 ttcggcgggc ggagagttga aaacggcgga catcgggggg agcctccggc ggtcagtggc
     1921 gggccgggcc gaggaagcgg ccggcggtta cggtcttgag cttcggcgca tcgaccgaga
     1981 cgaggtcgaa gcggatttca ttcgaggcgg ccttcagttc ctcggggtcg gcactgacgc
     2041 gggtcggcag atcgaagacc tcgccgctgc cgacgcggat ctcgcccggg tcctgcgtga
     2101 acttcaggcc gggcacgccg ctgacgccga ggcggtagcg gtgctcgcgc tcgtccatgt
     2161 tgatgatctt cagcgtgtag acgttttcga ccaggccctc gtcgttcatg cggtacagcg
     2221 cgttgcggtc gcggatcacg tcgagctgca gcggcacgcg cagcgcgatc gccaccagca
     2281 ggccgatggt cagcgccagc agcaggccgc cgtagaccat catgcgcggg cggatcacgt
     2341 gcggcacgtc gtgcatcagg ctgtgctcgg tcgtgtagcg gatcagcccg cgcgggtagt
     2401 tcatcttgtc catgatgtcg tcgcaggcat cgatgcaggc tgcgcagccg atgcactggt
     2461 actgcaggcc gttgcggatg tcgatgccgg tcgggcagac ctgcacgcac atcgtgcagt
     2521 cgacgcagga gccgaggccg gccgccttcg ggtcggtgcc gcgcttgcgc gagccgcgcg
     2581 gctcgccgcg ctcggcgtcg taggagatga ccagcgtgtc cttgtcgaac atcgcgctct
     2641 ggaagcgcgc atacggacac atgtacaggc agacctgctc gcgcatgaag ccggcgttgc
     2701 cccaggtcgc gaacgcgtag aagaacaccc agaaggtttc ccacggcccc cagtcgaacg
     2761 gccagaggcg ggcggcaaga tcgtgcatcg gcgtgaagaa gccgacgaag gtgaagccgg
     2821 tccacagcgc gaacagcgcc cagagcgagt gcttcaggag cttcttgcgg aacttgccgg
     2881 cgctccacgg cgccttgtcg agcttcatgc gcgcattgcg gtcgccttcg acccagtgct
     2941 cgatccacat gaaggtttcg gtccagaccg tctgcgggca ggcgtagccg caccacagcc
     3001 gcccggcgag cgccgtgaag aagaacagcg ccagcgccgc gatgatcagg agcagcgcca
     3061 ggaagaagaa gtcctgcggc cagaacaccc agccgaagat gtagaacttg cgcgccggca
     3121 ggtcgaagag caccgcctga cgaccatccc agggcagcca gggcagcccg taatagaggc
     3181 cgagcagcgc gaacaccgca tagatgcgca gcgtcgcgaa acgacccttg acctcacgcg
     3241 cgtagatctt cgcagtcttg gcgtagaagc tgcttttgcc ggaatcctgg ccctcgggcc
     3301 ggctcggtgt ttccgccgtc atgcaaacgt cctcgacatc ggcgaccggc gccgggggcg
     3361 gcgcggttca tcaggcacgc atgatgcttt cttcagcacg gcgccgcatt gcgaaaaatc
     3421 acgaatcgtc gtgcaaacac aggcttgcgc gcagcctcgt ggaatgccgg cagatgccgg
     3481 accgcagccg gctcagttct ggtccggatc gaagtcgcgt accgggcaga ccaccggcgt
     3541 gtgcaccggc tggcggaagt acagcgtcag cagcgccgag caggtgttca tcatccacag
     3601 cgcgaagaag ccgagcgagt acgtgccggt gcgctcgatg ccggtgacgc cgaggcagtg
     3661 cgcgatcgcc agcggatcga agatcgtcga caggatcacg gtgccggcgg ccgcgagcag
     3721 gaacgacggc cacagtaatg aagcgatgtg ctggactcgg gtgatcacga tgcttgcctc
     3781 cggtgcctga cgggtgcgga cgcgccgatg cgtgcggcgc ggtctcggat gcgggtggat
     3841 tccgccgctg cttaccgtag tcgctggccg cgccggcggc agtgatgaag cgcagtcggc
     3901 cggcggcacc ggcctcgccc gaaaaaaaac gccgctcccc ggggggagcg gcggcatgcg
     3961 gctgcggccc ggcaccgatg gccggaaacc gcgatcactg cgacaggctg tagacgtagg
     4021 cggccagcag gtggaccttg tcgttgccga ggaagtccgc gtgcgccggc atgcggccct
     4081 ggcgaccgtc ggcgatcgtc ttctcgacgg ccttcagcga accgccgtac agccaggtgt
     4141 tgtcggccag gttcggggcg cccatcaccg ggttgccctt gccttccgca ccgtggcaga
     4201 cggcgcagtt ctgcaggaac agcgccttgc cggcctcggc cttggcggcg tcgaccgact
     4261 ttccggacag gctcagcacg tagttggcaa cctgctcgac gccctgctcc ttcaggatcg
     4321 cgctccaggc cggcatcacg ccctggcgac cgttcatgat cgaggtcttg atcgtggccg
     4381 ggtccccgcc atacagccag tccttgtcgg cgaggttcgg gaagccgacc gcaccgtggg
     4441 cgtcggagcc gtggcagctg gagcagtagg tcgcgaacag ccggccaccc atcttcagtg
     4501 cctgcgggtc cttggcgacg gcggcgatgt ccatgccggc gtacttcttg aagatcgggt
     4561 cgtacttgtc ggcggcatcc ttcatctcgg actgccactg gccttcctgc gtccagccga
     4621 ggaagccggt gacgctgccg aggcccggat acagggccac gtagacgccg gtgaagacca
     4681 tggtcaggta gaacatccac agccaccagc gcggcatcgg attgttgaac tcggccaggt
     4741 cgccatccca ggtgtgaccc gtgacctcac cctgcaccga ctcgttcggc cggggcttgg
     4801 cggtccagcg gatcagccac cacatggcca gcgtgttgcc gaccacgatc agggtgatgt
     4861 aaaggctgac ggtcagactc atcttggcat ctctccttca gcgccggtct tcttgctcga
     4921 ccgtcctgcg cgcggtgtcc tcgtcggcga agggcaggct ggccgcgtcg tcgaaacgct
     4981 tgcggttgtg ccggctccac gcccaccaga cgatgccgat gaagatcacg aacatcagca
     5041 ccgtgaacac gctgcgcacc acggtcatga agaagtccac gggcgcttac ctcttgttgc
     5101 tgatcacggt gccgaggttc tgcagatacg cgatcagcgc ctcgatctcg gtcttgccct
     5161 gaaccgccgc cttcgcaccc tggatgtcgg cgtccgtgta cggcacaccg accaggcgca
     5221 gcgtctgcat cttcttgccg gtcagttcgc cgtcgagctt gccggtatcc agccacggga
     5281 agccgggcat gttggattcc ggcaccacgt cgcgcggatt gatcaggtgc gtgcgatgcc
     5341 agtcatcgga gtagcgaccg ccgacacgcg ccagatccgg accggtgcgc ttggagcccc
     5401 actggaacgg gtggtcgtag acgaactcgc cggccaccga gtagtggccg tagcgctcgg
     5461 tctcggcgcg gaacggacgc accatctgcg agtggcaggt gtagcagcct tcgcggatgt
     5521 agatgtcgcg gccttcgagc tgcagcgcgg tcagcggctt gaggccggcg accggctcgg
     5581 tcgtgtcctt caggaagaac agcggcacga tctcggcgag gccgccgaag gcaacgacga
     5641 tgaggatcag cacgaccatc aggccgatgt tgcgctcgac cttctcgtgg ccgaccgccg
     5701 gggcattgtc atgagccatg ttggtatgtc tcccttaagc ggcctgcggc gccggcgcgg
     5761 acaccggctg ttcgtcgccc tgcagcgtct tgtagacgtt ccaggccatc acgaacatgc
     5821 cgacgaggaa cagcagaccg ccgaggaagc ggaccgtcag gtacgggtac atgcgcacga
     5881 gggcttcgac gaagctgtag gtcagcgtgc cgtcctcgtt gaccgcacgc cacatcaggc
     5941 cctgcatgac gccggcgatc cacatcgagg cgatgtagag cacgatgccg atcgtgccgg
     6001 tccagaagtg gacctcgatc agcttggtgc tgtagatgcg gcggttgaac agctgcggga
     6061 tcagcgcata gacgcagccg atcgagatca gcgccaccca gccgagggcg ccggagtgca
     6121 cgtggccgat ggtccagtcg gtgtagtgcg acagcgcgtt gacggtcttg atcgacatca
     6181 tcggaccttc gaaggtcgac atgccgtaga acgacagcga gacgatcatg aagcgcagga
     6241 tcgggtcggt gcgcagctta tgccaggccc ccgagagcgt catgatgccg ttgatcatgc
     6301 cgccccagga cggcgcgagc agcatcagcg agaacaccat gccgatcgac tgcgtccagt
     6361 tcggcagcgc cgtgtagtgc aggtggtgcg ggccggccca catgtacagc gagatcagcg
     6421 cccagaagtg cacgaccgac agccggtacg agtacaccgg acgaccggcc tgcttcggca
     6481 cgaagtagta catcatcccg aggaagccgg cagtcaggaa gaagccgacc gcgttgtggc
     6541 cgtaccacca ctgcaccatc gcatcgatcg cgcccggata caccgagtac gacttggtca
     6601 ggctgaccgg cagctcggcg ctgttgacca cgtgcagcag cgcgacggtc aggatgaagg
     6661 cgccgtagaa ccagttggcg acgtagatgt gcggggtgcg gcgcttcatg atcgtgccga
     6721 agaacaggat cgcgtaggcg atccagacga cggcgatcag gatgtcgatc ggccactcca
     6781 gttcggcgta ctcc
//
LOCUS       DAOQJH010000032         6620 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_6476_length_6620_cov_3.799238, whole genome shotgun sequence.
ACCESSION   DAOQJH010000032 DAOQJH010000000
VERSION     DAOQJH010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 6620)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6620)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6620
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6476_length_6620_cov_3.799238"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1357)
                     /gene="xrtA"
                     /locus_tag="PLQ18_01445"
     CDS             complement(<1..1357)
                     /gene="xrtA"
                     /locus_tag="PLQ18_01445"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase A"
                     /protein_id="HNE00342.1"
                     /translation="MSDPALVSVTAADAQAPLTDAAPVPARGWPVTLAALTVALLATI
                     GLYWPTATGMASIWWRSDTFAHGMLVVPIVLYMLWTRRAEIAPVAPRPAYPGLALLAL
                     LAAGWFIADSAGVGVGSQLCLVAMFPALVWTLCGRAVVAAAAFPLAYLIFAVPIGEGL
                     VPPLQDFTAHFTVRAVRLTGIPVYAEGWYLMLPSGTFQVAEACSGIRYLIASLALGCL
                     YAYLTYRSPWRRFLFIVFAAIVPVLANGLRAFGIVMIAHFTKMEYATGADHLVYGWLF
                     FGLVIGLMFWVGRFWEEDPSVPPVRQTGLTTAAPAPAAAAQPWPALVLSLCVLALAPL
                     ALWRSDAQPVASVPVLTAPAVAGWNGPKPAEWATTYTGQTRTLVAQYDDGRGHDLRLW
                     VAAWAGGHDSSRMISSVHLPYKPGEWVQGADRVRRVPTAAGEREVYEVPVIAGERRLT
                     IW"
     gene            complement(1354..2553)
                     /locus_tag="PLQ18_01450"
     CDS             complement(1354..2553)
                     /locus_tag="PLQ18_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018874162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03087 family PEP-CTERM/XrtA system
                     glycosyltransferase"
                     /protein_id="HNE00343.1"
                     /translation="MEELLLLVHRIPYPPNKGDKIRSYHLLRFLAARYRVHLGAFVDD
                     PADFAHATQLQAMCASLCLRPLDARRAKLRAFSGLLTGEALSLPYYRDRGFARWVDGV
                     LARGVRRVVVFSSAMAQYVLDRPGLELVTDFCDVDSDKWRQYAQVQRWPQSWVYRREA
                     ARLLAFDRRVAAASRAALFVSEPEAALFRQLAPEAAGHTGFFHNGVDTAYFDPATTYE
                     NPYPAGGRPLVFTGAMDYRANVDAVDWYARAVLPIVRAAQPDAQFWIVGSRPAAAVQA
                     LGALPGVSVTGAVADVRPYVAHAAAAVAPLRIARGVQNKVLEAMAMARPVLATTAAMD
                     GIVPNPRFDALVADEAAPLAAHTIRLLADGDRDGLGALGRAWVLAHYDWDTNLRPVAG
                     WLEREAA"
     gene            complement(2566..3630)
                     /locus_tag="PLQ18_01455"
     CDS             complement(2566..3630)
                     /locus_tag="PLQ18_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013034138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FemAB family PEP-CTERM system-associated
                     protein"
                     /protein_id="HNE00344.1"
                     /translation="MSVAATMSTAAGSIAVRRLQPADHARWDAFVAACPEATFFHRAG
                     WAEVLQRAFAHDAHFLYAERDGVIVGVLPLGHLRSRLFGNALISVPFCVYGGIAATDE
                     EVRRPLADAACRLAQELGVDYLELRHRRRQFPDWPCKDGLYVTFRKPILPDEDANLKA
                     IPRKQRAMVRKGIEAGLEGRIEAGVDNLFLAYSESVRNLGTPVLPKRYFQVLREVFGS
                     DCEVLTIRQGETLVASVLSFYFRDEVLPYYGGGTAQAREVKGNDFMYWDLLRRSCARG
                     LRLFDYGRSKNGTGSYSFKKNWGFEPEPLYYEFHLVRATRLPDINPLNPKYRLFIAAW
                     QRLPLWLSRRLGPWLARSLG"
     gene            complement(3627..4484)
                     /locus_tag="PLQ18_01460"
     CDS             complement(3627..4484)
                     /locus_tag="PLQ18_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002809078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3473 domain-containing protein"
                     /protein_id="HNE00345.1"
                     /translation="MQATRPVNAMTVDVEDWFQVSALTPWVRREDWETLPRRVGANTH
                     RVLDLFAGAGVRATFFTLGWVAERHPELVRRIVAEGHELASHGWQHVRVIHQSPEEFR
                     ADVIRTKALLEDLGGVPVRGYRAASYSIGARNLWALEILAETGHVYSSSIFPGSHDLY
                     GMPGAPRFRFRPDNAPALLEVPITTVELGTRRLPAAGGGFFRLFPYALSRALLRRVNR
                     EDRESGLFYFHPWEIDPAQPRPAGVSGRARLRHYLNLGRTESRLRRLLADFSWDRMDR
                     VFLDPESVA"
     gene            complement(4502..5590)
                     /locus_tag="PLQ18_01465"
     CDS             complement(4502..5590)
                     /locus_tag="PLQ18_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XrtA-associated ATPase"
                     /protein_id="HNE00346.1"
                     /translation="MYTQYYGFSAKPFQLSPDPRFFFGSSGHKRALAYLRYGLSQGEG
                     FIVVTGGIGTGKTTLVRNLFAELDPATVVAAQLVTTQLEADDLLRMVVASFGLAHESV
                     SKASLLKYFEDYLRSCAREGRRVLLIVDEAQNLPARSLEELRMLSNFQVGEKPLLQSF
                     LLGQDEFRATLGGPGLEQLRQRVIASCHLHPLGADETRQYIEHRLRVVGWQGRPAFTE
                     AAFRLIHETTQGIPRRINVFCDRVLLYGFLEELTEFGEEAVRATRSEFAQEVFANSAG
                     AARTAEAPAPRPVTDPAAGDALAARLDRIERAVSGLLQVQAGGEESAEAERRLGELEA
                     SVGMLKEGFLRLANVIDRHFETDRSEAS"
     gene            complement(5634..>6620)
                     /locus_tag="PLQ18_01470"
     CDS             complement(5634..>6620)
                     /locus_tag="PLQ18_01470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE00347.1"
                     /translation="SRSVEGYADGNLGNDEREHAEASVRQGISREWALLGRVGLENNS
                     THDISEQEAANGNYWSGGLGWTPSRVLGLDAWYGSRDKELTLNWNPSERTSLVLSWRD
                     RAVGLVTGPSWNLDFRHRTAYSDWTARYSEEVTNSQGVARKDSNFLSFVNPQTGEVVY
                     FDPATGRLLPASSAFSLNNESFLRKRFSLGNSYQRGHSQFNSNVFHESREYEASGRKE
                     TSLGLDASWSWQFAPRTRSVLAVNWERVSGDDSSSGSGSGSDSTGSSGDSDYLTGRIG
                     LSHQLSADADALIEYAHTTSDGGSGGFGSSGGGSSSQSYDENRLTLSINMRF"
BASE COUNT         1063 a         2293 c         2345 g          919 t
ORIGIN      
        1 tccagatcgt cagcctgcgc tcgccggcga tgaccggcac ctcgtagacc tcacgctcgc
       61 cggccgccgt cggcacgcgc cgcacgcgat cggcgccctg cacccattcg ccgggcttgt
      121 acggcaggtg gaccgaggaa atcatccggc tgctgtcgtg gccgccggcc caggccgcca
      181 cccacagccg caggtcgtgg ccgcgcccat cgtcgtattg cgctacgagc gtgcgggtct
      241 ggccggtata ggtcgtcgcc cactcggccg gcttcgggcc gttccagccg gccacggccg
      301 gcgccgtcag caccggcacg gacgccaccg gctgcgcgtc gctgcgccac agcgccagcg
      361 gtgcgagcgc aagcacgcag aggctcagca ccagcgccgg ccacggctgc gcagccgccg
      421 ccggtgccgg cgctgccgtc gtcagaccgg tctgccggac gggcggcacg gacgggtcct
      481 cctcccagaa gcgtccgacc cagaacatca ggccgatcac gaggccgaag aacagccagc
      541 cgtagaccag atggtcggcg ccggtcgcgt actccatctt cgtgaagtgc gcgatcatga
      601 cgatgccgaa ggcccgcagg ccgttggcaa gcaccggcac gatggccgcg aacacgatga
      661 acaggaagcg ccgccacggc gagcggtagg tcaggtaggc gtacaggcag ccgagcgcga
      721 gcgacgcgat cagataacgg atgccgctgc aggcctcggc gacctggaag gtgccgctcg
      781 gcagcatcag ataccagcct tccgcgtaca ccgggatgcc ggtcaggcgc accgcccgca
      841 ccgtgaaatg cgccgtgaag tcctgcagcg gcgggaccag accttcgccg atcggcaccg
      901 caaagatcag gtaggcaagc gggaacgccg ctgccgcgac cacggcccgg ccgcacagcg
      961 tccagaccag cgccggaaac atcgcgacca gacagagctg gctgccgacg ccgacgccgg
     1021 cgctgtcggc gatgaaccag ccggcggcaa gcagcgcgag cagcgcgagc cccggatacg
     1081 ccggccgcgg tgccaccggc gcgatctcgg cacgccgcgt ccagagcatg tacagcacga
     1141 tcggcacgac cagcatgccg tgcgcgaagg tgtccgagcg ccaccagatc gaggccatgc
     1201 cggtcgccgt cggccagtac aggccgatgg tcgcgagcag ggccacggtc agcgccgcca
     1261 gcgtcaccgg ccagccgcgg gccgggaccg gagctgcatc cgtcagcggc gcctgcgcat
     1321 cggcagcggt gacgctgacc agagccggat cgctcatgcg gcctcccgtt cgagccagcc
     1381 ggccaccggc cggaggttgg tgtcccagtc gtagtgcgcg agcacccagg cgcgcccgag
     1441 cgcgccgagg ccgtcgcggt cgccgtcggc gagcagccgg atcgtgtgcg ccgcgagcgg
     1501 cgcggcctca tcggccacca gcgcgtcgaa gcgcggattc ggcacgatgc cgtccattgc
     1561 cgccgtcgtc gccagcaccg gcctggccat cgccatcgct tcgagcacct tgttctgcac
     1621 gccgcgcgcg atgcgcagcg gcgcgaccgc cgccgcagca tgcgcgacat aaggccgcac
     1681 gtcggccacc gcgccggtca cgctcacgcc cggcagcgcg ccgagcgcct gcacggctgc
     1741 ggccggtcgg ctgccgacga tccagaactg cgcgtccggc tgcgcggcgc gcacgatcgg
     1801 cagcaccgcg cgcgcgtacc agtcgacggc atcgacgttg gcgcggtaat ccatcgcgcc
     1861 ggtgaacacc agcggccggc cgccggccgg atacgggttc tcgtatgtcg tggccggatc
     1921 gaagtacgcg gtatcgacgc cgttgtggaa gaagccggtg tggccggccg cctccggggc
     1981 gagctgccgg aacagcgccg cctcgggctc ggacacgaac agcgccgcgc ggctcgccgc
     2041 ggcgacgcgc cggtcgaagg cgagcaggcg cgcggcttcg cgccggtaga cccagctctg
     2101 cggccagcgc tgcacctgcg cgtactggcg ccacttgtcg gagtcgacat cgcagaagtc
     2161 ggtgacgagt tcgagcccgg gcctgtcgag cacgtactgc gccatcgccg acgagaacac
     2221 gacgacacga cgcacgccgc gggcgagcac gccgtcgacc cagcgggcga agccgcgatc
     2281 acggtaatac ggcagcgaca gcgcctcgcc ggtgagcagc ccgctgaatg cacgcagctt
     2341 cgcgcgccgg gcatcgagcg ggcgcaggca gaggctcgcg cacatcgcct gcagctgcgt
     2401 ggcgtgcgcg aagtcggccg gatcgtcgac gaaggcgccg agatgcacgc ggtagcgcgc
     2461 cgccaggaaa cgcagcaggt gataggagcg gatcttgtcg cccttgttcg gcggatacgg
     2521 gatgcggtgc accagcagca ggagctcttc catgcccgtt cccgttcagc cgagactgcg
     2581 cgccagccag ggcccgagcc ggcggctcag ccacagcggc aggcgctgcc aggcggcgat
     2641 gaacagccgg tatttcgggt tcagcggatt gatgtccggc agccgcgtcg cccgcaccag
     2701 atggaattcg tagtacagcg gctcgggctc gaagccccag ttcttcttga agctgtacga
     2761 accggtgccg ttcttgctgc gcccgtagtc gaacaaacgc agaccgcgcg cgcagctgcg
     2821 ccgcagcaga tcccagtaca tgaagtcgtt gcccttgacc tcgcgcgcct gcgcggtgcc
     2881 gccgccgtag tacggcagca cctcgtcgcg gaagtagaag ctcagcacgc tcgcgaccag
     2941 cgtctcgccc tggcggatgg tcagcacctc gcagtcgctg ccgaacacct cgcgcagcac
     3001 ctggaagtag cgcttcggca gcaccggcgt gccgaggttg cgcacgctct cggagtaggc
     3061 gaggaacagg ttgtcgacgc cggcctcgat gcggccttcg agcccggcct cgatgccctt
     3121 gcgcaccatc gcccgctgct tgcgcgggat cgccttcagg ttggcgtcct cgtcgggcag
     3181 gatcggtttg cggaaggtca cgtacaggcc gtccttgcac ggccagtccg ggaactggcg
     3241 gcggcggtgg cgcagttcga ggtaatcgac gccgagctcc tgcgcgagcc ggcaggcggc
     3301 atcggcgagc ggccggcgca cctcctcgtc ggtcgcggcg atgccgccgt agacgcagaa
     3361 cggcaccgag atcagcgcat tgccgaacag gcggctgcgc aggtgcccga gcggcagcac
     3421 gccgacgatg acgccgtcgc gctcggcgta caggaagtgc gcgtcgtgag cgaaggcacg
     3481 ctgcaggacc tcggcccagc cggcccggtg aaagaaggtc gcctccggac aggcggcgac
     3541 gaaggcatcc cagcgggcgt gatcggcggg ctgcaggcgg cgcacggcga tgctgcccgc
     3601 agcggtgctc atggtggcag cgacgctcat gcgacggact ccgggtcgag gaagacgcga
     3661 tccatccggt cccagctgaa atcggcgagc aggcgccgca gccgggactc ggtgcgaccg
     3721 aggttcaggt agtgccgcaa gcgcgcgcgc ccggacacgc cggccggccg cggctgcgcc
     3781 ggatcgattt cccaggggtg gaagtagaac agcccggact cgcgatcctc gcgattgacc
     3841 cgccgcagca gcgcacgcga cagcgcatac gggaacagcc ggaagaaacc gccgccggcc
     3901 gccggcagcc ggcgcgtgcc gagctcgacc gtcgtgatcg gcacctccag cagcgccggc
     3961 gcgttgtcgg gccggaagcg gaagcgcggc gcaccgggca tgccgtagag gtcgtgactg
     4021 cccgggaaga tgctggagct gtagacgtgc ccggtctcgg cgaggatttc gagcgcccac
     4081 aggttgcgcg ccccgatcga gtagctcgcg gcgcggtagc cgcgcaccgg cacgccgccg
     4141 aggtcttcga gtagggcctt cgtgcggatc acgtcggcgc ggaattcctc cgggctctgg
     4201 tggatcacgc gcacgtgctg ccagccgtgg ctcgccagtt catggccttc ggcgacgatg
     4261 cggcgcacga gctcggggtg acgctcggca acccagccga gcgtgaagaa ggtcgcccgc
     4321 acgccggcac cggcgaacag gtcgagcacg cggtgcgtgt tggcgccgac gcgccgcggc
     4381 agggtttccc agtcctcgcg ccgcacccag ggcgtgagcg ccgagacctg gaaccagtcc
     4441 tcgacatcga ccgtcatcgc gttgacggga cgggtggctt gcatcggcgg tctccgcagg
     4501 ctcaggaggc ttcggaccgg tcggtctcga agtgccggtc gatgacgttc gcaagacgca
     4561 ggaagccttc cttgagcatg ccgacgctcg cttcgagctc gccgagccgg cgctcggcct
     4621 cggccgattc ctcgccgccg gcctgcacct gcagcaggcc gctgacagcg cgctcgatgc
     4681 gatcgaggcg tgccgccagc gcatcgccgg ccgccgggtc cgtcaccggt cgcggggccg
     4741 gggcctcggc cgtgcgggca gccccggcgc tgttcgcgaa cacctcctgc gcgaactcgc
     4801 tgcgcgtggc gcgaacggct tcttccccga actcggtgag ttcctccagg aagccgtaga
     4861 gcagcacgcg atcacagaac acgttgatgc gccgcggaat gccctgcgtg gtctcgtgga
     4921 tcagccggaa ggcggcctcg gtgaaggccg gccggccctg ccagccgacc acgcgcaggc
     4981 ggtgctcgat gtactggcgg gtctcgtcgg ccccgagcgg gtgcaggtgg caggaggcga
     5041 tcacgcgctg gcgcagctgt tcgaggccgg ggccgccgag cgtggcgcgg aactcgtcct
     5101 ggccgagcag gaagctctgc agcagcggtt tctcgccgac ctggaagttc gacagcatgc
     5161 gcagctcctc cagcgagcgc gccggcaggt tctgcgcctc gtcgacgatc agcagcacgc
     5221 gccggccctc gcgcgcacac gagcgcaggt agtcctcgaa gtacttcagc agcgaggcct
     5281 tggacaccga ttcgtgcgcg agcccgaagg acgcgaccac catgcgcagc aggtcgtcgg
     5341 cttcgagctg ggtcgtgacc agctgcgcgg cgacgacggt ggccgggtca agctcggcga
     5401 acaggttgcg caccagcgtc gtcttgccgg tgccgatgcc gccggtgacg acgatgaagc
     5461 cctcgccctg cgacagaccg tagcgcagat aggcgagcgc acgcttgtga ccgctgctgc
     5521 cgaagaagaa gcgcggatcg ggactgagct ggaagggttt ggcgctgaag ccgtagtact
     5581 gcgtgtacac gcgtctcgat tcccgtgatg cggcgcaagc cggggcaggc gcttcagaag
     5641 cgcatgttga tgctgagggt cagccggttc tcgtcgtagg actgcgagct gctgccaccg
     5701 ccgctgctgc cgaaaccgcc gctgccgccg tcgctggtgg tgtgggcgta ttcgatcagc
     5761 gcatcggcat cggccgagag ctggtgactg aggccgatgc ggccggtcag atagtcactg
     5821 tcgccggaac tgccggtcga gtcggaaccg gagccggaac cggaactgct gtcgtcgccg
     5881 gacacgcgtt cccagttgac ggcaagcacg ctgcgggtac gcggtgcgaa ctgccagctc
     5941 cagctggcat cgaggccgag ggaggtttcc ttgcgtcccg aggcttcgta ttcgcggctc
     6001 tcgtgaaaga cgttggaatt gaactggctg tggccgcgct ggtagctgtt gccgagcgag
     6061 aagcgcttgc gcaggaagga ttcgttgttc agcgagaacg ccgacgatgc cggcagcagc
     6121 cgcccggtgg ccggatcgaa atagacgacc tcgccggtct gcggattcac gaacgacagg
     6181 aagttgctgt ccttgcgcgc gacgccctgc gagttcgtga cttcctcgga atagcgcgcg
     6241 gtccagtccg aataagccgt gcggtgacgg aaatcgagat tccagctcgg tccggtcacg
     6301 aggccgacgg cacgatcccg ccagctcagc accagtgagg tgcgctcgga cggattccag
     6361 ttcagcgtca gttccttgtc gcgcgagcca taccaggcgt cgaggcccag cacgcgtgac
     6421 ggcgtccagc cgaggccacc gctccagtag ttgccgttgg cggcttcctg ttcgctgatg
     6481 tcatgggtcg agttgttttc cagaccgacc cggccgagca gcgcccattc gcgactgatg
     6541 ccctggcgca cgctcgcttc ggcgtgctcc cgctcgtcgt tgccgagatt gccatcggcg
     6601 taaccttcga ccgagcgcga
//
LOCUS       DAOQJH010000033         6579 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_6536_length_6579_cov_3.487584, whole genome shotgun sequence.
ACCESSION   DAOQJH010000033 DAOQJH010000000
VERSION     DAOQJH010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 6579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6579
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6536_length_6579_cov_3.487584"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            136..912
                     /locus_tag="PLQ18_01475"
     CDS             136..912
                     /locus_tag="PLQ18_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007079918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HNE00348.1"
                     /translation="MRTRRGAGALALGLALLLALPAAQALTWRELDFDGAHYTIAELD
                     LARDPLALYWRDPADGTPYGTLGALAEHERQRGRRLRFATNAGIYAEGQVPLGLTVEE
                     GVEQVPLNRARGGGNFFLQPNGVFAVWGRSHAAVLNSAEYARRRGPLPRLATQSGPML
                     LIDGRIHPRFQRDSDSLKLRSGVCVPAPGKVSFAISEGLVNFHAFARLFRDALGCRDA
                     LYLDGSISQMHLDGRDGLAGADRWRTRPFAALLGVSVPAD"
     gene            975..1475
                     /locus_tag="PLQ18_01480"
     CDS             975..1475
                     /locus_tag="PLQ18_01480"
                     /inference="COORDINATES: protein motif:HMM:NF023802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="papain-like cysteine protease family protein"
                     /protein_id="HNE00349.1"
                     /translation="MALIDVNHILPKEGYGQFTSTTCWYASYRLLHAWKQADESTIRP
                     SLEAGGLNWKELTTRGIYPEEWPVAGSRLGLCGWEGRLVKAWDDEMIVYALKGYGPLY
                     FTWDYGSSGHAVVIGGFDKKLNQFKVWNPYNRFEPGTVEIDWFTPDAFRERLHAGRWA
                     LQAWWR"
     gene            1722..2414
                     /locus_tag="PLQ18_01485"
     CDS             1722..2414
                     /locus_tag="PLQ18_01485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HNE00350.1"
                     /translation="MKHFTYALALAGLLGLNAGAHAAIVSVDVDRFEEGFQNIQDTTL
                     DSAPVTDTVSLSPANGVATSRTVAINVLSYSAGATNQLTVTADPTVSLLDVINEVGED
                     SEVTVSWVIPASLIPNDAFNTFFRIAVLQSDGNDLSVEFLLNGTALGNFAVPGNTFNQ
                     DLLFTASAAQINAGGTLEMIINGQAGWDADFDDIGFAYETPTAAPEPVSLALFGAGLI
                     GMRRLRRRAIPV"
     gene            2458..3165
                     /locus_tag="PLQ18_01490"
     CDS             2458..3165
                     /locus_tag="PLQ18_01490"
                     /inference="COORDINATES: protein motif:HMM:NF019225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HNE00351.1"
                     /translation="MKIKNLLPALAASAALAASAPAAAVLIEVNIDDFNVGSHMASDN
                     TVGNGGVTSALSSLDTAHVIGGTRTLVDNLTAGIIPYQSSTRVDATVGQLIINNGVGE
                     VTGSSVTWALPANLLPTTGSLSNFQFHMALTGKDNNAVSVSVLLNGSTIGTSSLPTAQ
                     VTTLQDLYFSSTIASFNAGGTLQLVFSGNAGWDASFDTFGFQYDYTPPPPVTVPVPAT
                     LALFGIGAVALHRRRRC"
     gene            3304..3810
                     /locus_tag="PLQ18_01495"
     CDS             3304..3810
                     /locus_tag="PLQ18_01495"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNE00352.1"
                     /translation="MRFRLPVFESALVLLAAVTAPAAELDDIRRLADEGQQDAALRRL
                     DERLVRDPSDVPARFLKGSILLQTGKLGEAREAFDEVTRRAPKLPEPYNNLAVIYAAQ
                     GDYEKARQSLYTAAQQAPDYAAAQINLGDLYVKLAVDAYRRALELNPADEASRAKLLA
                     LDAMFRRQ"
     gene            3807..4310
                     /locus_tag="PLQ18_01500"
     CDS             3807..4310
                     /locus_tag="PLQ18_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004364516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidohydrolase family
                     protein"
                     /protein_id="HNE00353.1"
                     /translation="MNATDPDPYARARAVGQALAARGLRLACAESCTGGGIAHALTAV
                     PGSSAWFERGFVTYSNAAKEQLLGVQPATLAAHGAVSAETVIEMALGTLAHAEAEFAV
                     AVSGIAGPDGGSADKPVGTVWIAWARAGREPQARRFVFAGDRAMVRAAAVLAALDGVL
                     ERLDGDP"
     gene            4307..4837
                     /gene="thpR"
                     /locus_tag="PLQ18_01505"
     CDS             4307..4837
                     /gene="thpR"
                     /locus_tag="PLQ18_01505"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HNE00354.1"
                     /translation="MTQAPAPGRRLFLALWPDPAVRAELARLARRLTPEHVPAANLHL
                     TLVFLGFTPPEREAGCRAALEGLRPAPFTLELDRLGCFRGPRVLWLGPSQIPPALTAL
                     VGELQRRLAACAHEPDPRPFAAHVTLARRFSGSAPAYPLPSPIRWPAARIVLAESRSQ
                     PGGVRYEPLADWPVGL"
     gene            4918..5973
                     /gene="recA"
                     /locus_tag="PLQ18_01510"
     CDS             4918..5973
                     /gene="recA"
                     /locus_tag="PLQ18_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HNE00355.1"
                     /translation="MDDNRRKALAAALGQIEKQFGKGSVMRLGDANAARDIDVISTGS
                     LGLDIALGVGGLPRGRVVEIYGPESSGKTTLTLQVIAEAQKLGGTCAFVDAEHALDPI
                     YAAKLGVKVADLLVSQPDTGEQALEITDMLVRSAAVDVVVVDSVAALTPKAEIEGEMG
                     DSHVGLHARLMSQALRKLTGNINRSNTLVIFINQIRLKIGVMFGNPETTTGGNALKFY
                     ASVRLDIRRTGSIKKGDEVLGSETRVKVVKNKVAPPFKQAEFEILYGEGISRLGEIID
                     LGVKAALIDKSGAWYSYGGQRIGQGKENVRQFLKDNPAIAAELDAKVRAHYLSNPDAL
                     PTVPVGDDEAGGTGDEF"
     gene            5963..6454
                     /locus_tag="PLQ18_01515"
     CDS             5963..6454
                     /locus_tag="PLQ18_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012702371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HNE00356.1"
                     /translation="MSSDEPGDPDDPEEAGALLYRTALALLARREHTRLELAAKLARH
                     GAGAGIIAAVLDRLQAEQLLSEQRFAEVYAATRFDRGYGPLRLRAELRERGVSEAIVS
                     DILAGFDAQWTARLAALARRRFGPQPPKDARERAQRTAFLRGRGFTGEQIARLMRRDP
                     GDD"
BASE COUNT         1001 a         2311 c         2251 g         1016 t
ORIGIN      
        1 ggcgctggtc ggctcgatcg ggccggcgct gacgatcgcg atgggcgcgg ccttcctcgg
       61 cgaatcgatc ctgccgctgc agtggctcgg cgcggcgctg gtggtcggcg gcgtgctgct
      121 ggtgggctcg gggcgatgag gacgcgacgc ggcgcaggcg ccctcgcgct cgggctcgcc
      181 ctgctgctgg cgctgccggc ggcgcaggcg ctcacgtggc gcgaactcga cttcgatggt
      241 gcgcattaca cgatcgccga actcgatctc gcccgcgatc cgctggcgct gtactggcgt
      301 gatccggccg acggcacgcc gtacggcacg ctcggggcgc tcgccgagca cgaacgccag
      361 cgcggccggc gcctgcgctt cgcgaccaat gccggcatct acgccgaagg ccaggtgccg
      421 ctcgggctga cggtcgagga gggcgtcgaa caggtgccac tcaaccgcgc ccgcggtggc
      481 ggcaacttct tcctgcagcc gaacggcgtg ttcgcggtct gggggcgcag ccacgcggcc
      541 gtgctgaaca gcgccgagta cgcgcgccgg cgcggcccgc tgccgcggct ggcgacgcag
      601 tccggaccga tgctgctcat cgatggccgc atccatccgc gcttccagcg cgattcggac
      661 agcctgaagc tcaggagcgg cgtctgcgtg ccggcgccgg gcaaggtgag cttcgcgatc
      721 agcgagggac tcgtgaactt ccacgccttc gcgcggctgt ttcgcgatgc gctcggctgt
      781 cgcgatgcgc tgtacctcga cggctcgatc tcgcagatgc acctcgacgg ccgcgacggc
      841 ctcgccgggg ccgatcgctg gcgcacgcgc ccgttcgcgg cgctgctcgg cgtgtcggtg
      901 ccggcggact gagggcgcta cgttggaggc gatgccgtgc gcaccccgcg catggccgtc
      961 tccggagcgc cgtcatggcc ctgatcgacg tgaatcacat cctgccgaag gaaggctacg
     1021 gccagttcac cagcacgacc tgctggtacg cgagctaccg cctgctgcac gcgtggaagc
     1081 aggccgatga gtcgacaatc cgcccgagcc tcgaagccgg cggcctgaac tggaaggagc
     1141 tgacgacgcg cggcatctat cccgaggagt ggccggtcgc cggcagccgg ctcgggctct
     1201 gcggctggga agggcggctg gtgaaggcct gggatgacga gatgatcgtc tacgcgctca
     1261 agggctacgg gccgttgtac ttcacgtggg actacggcag ctcggggcat gccgtggtca
     1321 tcggcggctt cgacaaaaag ctcaaccagt tcaaggtctg gaacccgtac aaccgcttcg
     1381 agcccggcac ggtcgagatc gactggttca cgccggatgc ctttcgcgag cgcctgcacg
     1441 ccggacgctg ggcgctgcag gcctggtggc gctgatccgc cggactcgtc agcttccttt
     1501 acaggaaagc tgcagtaagc atggttgcct taaaacaaat caatatcctg cggacatggc
     1561 tcggaaattg aatatcggcc tcgacggctg ttgcgctgcg tcaagattcc tgacagtcga
     1621 gcccgggtat gcaagaccgg gccgaagcgc aacaccaggt ggataacccc gcttccccgt
     1681 tgctggaggc gggccctgac gcagggacaa cgggaggcga catgaagcac ttcacttacg
     1741 cgctggcgct ggccggcctg ctcggcctga atgccggcgc acatgcggcg atcgtgtcgg
     1801 tcgatgtcga ccgcttcgag gaaggcttcc agaacatcca ggacacgacc ctcgacagtg
     1861 cgccggtgac ggacaccgtg tcgctgagcc ctgcgaacgg ggtggccacc tcgcgcacgg
     1921 tcgcgatcaa cgtgctcagc tacagcgccg gtgcgaccaa ccagttgacg gtcacggccg
     1981 atccgaccgt gagcctgctc gatgtgatca acgaggtcgg cgaggactcc gaggtcaccg
     2041 tgtcgtgggt gatcccggcg agcctgatcc cgaacgacgc cttcaacacc ttcttccgga
     2101 tcgcggtgct gcagtccgac ggcaacgacc tgtccgtcga gttcctgctc aacggcacgg
     2161 cgctcggcaa cttcgcggtg cccggcaaca ccttcaatca ggatctgctg ttcacggcca
     2221 gtgccgcgca gatcaacgcc ggcggcacgc tggaaatgat catcaacggt caggccggct
     2281 gggatgccga cttcgacgac atcggctttg cctacgaaac cccgacggct gcgccggaac
     2341 cggtttcgct ggcgctgttc ggagcaggcc tgatcggcat gcggcgcctg cgacgccgcg
     2401 cgatcccggt ctgagtgcca ttccccccaa ccggcacggg tcttcaggaa gatacgcatg
     2461 aaaatcaaga atctgcttcc tgcgctggcg gcttcggcgg cgctggcggc gagcgcgccg
     2521 gcagcggcgg tgctgatcga ggtcaacatc gatgacttca acgtcggcag ccacatggct
     2581 tccgataaca cggtcggcaa cggcggcgtg accagtgccc tgagcagcct cgacacggcg
     2641 cacgtcatcg gcggtacgcg cacgctcgtc gacaacctga cggccggcat cattccgtat
     2701 cagagctcga cgcgcgtcga tgccacggtc ggccagctga tcatcaacaa cggcgtcggc
     2761 gaggtgaccg gcagcagcgt gacctgggca ttgccggcca acctgctgcc gacgaccggc
     2821 agcctcagca acttccagtt ccacatggcg ctgaccggca aggacaacaa tgcagtttcg
     2881 gtcagcgtgc tgctgaacgg ttcgacgatc ggcacgagtt cgctgccgac tgctcaggtc
     2941 accacgctgc aggatctgta cttctcctcc accatcgcca gcttcaatgc cggtggcacg
     3001 ctgcagctgg tgttcagcgg caacgccggc tgggatgcct ccttcgacac cttcggcttt
     3061 cagtacgact acacgccgcc gccgccggtc accgtgccgg tgccggcaac gctggcgctg
     3121 ttcggaatcg gtgctgtggc gttgcaccgt cgccgccgct gctgaccgcg ctgcgagcgg
     3181 cctgcctgtc cgcgcccccc gaggctccgc ccgcgggggg cgcggcgttt ccggggcggg
     3241 ttgcggccgg gcacgtgttg taggatgcag cgcaccgtcc gaccgctcga aggaacgtcc
     3301 tgcatgcgct tccggcttcc cgtatttgag tctgcgctcg tcctgctcgc tgccgtcacc
     3361 gcgccggcgg ccgaactcga cgacatccgc cgtctggccg acgaaggcca gcaggacgcg
     3421 gcgctgcgcc ggctcgatga acgcctcgtg cgtgatccgt ccgacgtgcc ggcgcgcttc
     3481 ctgaagggtt cgatcctgct gcagaccggc aagctcggcg aagcgcgcga ggccttcgac
     3541 gaagtcacgc gccgcgcgcc gaagctgccg gagccgtaca acaatctggc cgtgatctac
     3601 gccgcgcagg gcgactacga gaaggcccgc cagtcgctgt acacggctgc gcagcaggcg
     3661 ccggactacg cggccgcgca gatcaacctc ggcgacctgt acgtgaagct cgccgtcgat
     3721 gcctaccgcc gggcgctgga actcaacccg gccgatgagg cctcgcgcgc caagctgctg
     3781 gcgctcgacg cgatgttccg gcgccagtga acgcgacgga tcccgatccg tacgcacgcg
     3841 cccgggccgt cggtcaggcg ctcgcggccc gcggcctgcg gctggcctgt gccgaatcct
     3901 gtaccggcgg cggcatcgcc cacgcgctga ccgcagtgcc cggctcctcg gcgtggttcg
     3961 agcgcggctt cgtcacctat tcgaacgccg ccaaggagca gctgctgggc gtgcagccgg
     4021 cgacgctggc cgcgcacggc gccgtcagcg ccgagaccgt catcgagatg gcgctcggca
     4081 cgctcgcgca cgccgaggcc gagttcgccg tcgcggtctc cggcatcgcc ggcccggacg
     4141 gcggcagcgc cgacaagccg gtcggcacgg tctggatcgc ctgggcgcga gccgggcgcg
     4201 agccgcaggc caggcgcttc gtgttcgcgg gcgaccgggc catggtgcgc gcggcggcgg
     4261 tgctggcggc gctcgatggc gtgctcgaac gactggatgg cgatccgtga cgcaggcacc
     4321 tgcgcccggc cggcgcctgt tcctcgcact gtggccggac ccggccgtgc gcgcggagct
     4381 ggcacgactt gcgcgccggc tgacgccgga acacgtgccg gcggcgaacc tgcatctgac
     4441 gctggtcttc ctcggcttca cgccgccgga gcgcgaagcc ggctgccggg ccgcactcga
     4501 gggcctgagg cctgcgccgt tcacgctcga actcgaccgg ctcggctgtt ttcggggccc
     4561 gcgcgtgctg tggctcggtc cgtcgcagat accgccggcg ctgacggcgc tggtcgggga
     4621 actgcagcgc cggctcgcgg cctgcgctca tgagcccgat ccccggccgt ttgccgcgca
     4681 cgtgacgctc gcccgtcgat tttccgggtc tgcacccgca tatccgctgc catcgccgat
     4741 ccgctggccg gctgcgcgca tcgtgctcgc cgaatcccgc agccagcccg gcggcgtgcg
     4801 ctacgaaccg ctggccgact ggccggtcgg gctctaggtc ccccgcaagt ggtgtggaat
     4861 actccacgca ccttgcgccg ttcgacatcc gttcccaccg ttccccggga gtacgcaatg
     4921 gacgataacc gcaggaaggc gctcgccgct gcgcttggcc agatcgagaa gcagttcggc
     4981 aagggctcgg tgatgcgcct cggcgacgcc aatgccgcgc gcgacatcga tgtgatctcc
     5041 accggctcgc tcggcctcga catcgcgctc ggcgtcggcg gcctgccgcg cggtcgtgtc
     5101 gtcgagatct acgggccgga atcctcgggc aagaccacgc tgacgctgca ggtcatcgcc
     5161 gaggcccaga agctcggcgg cacctgcgcc ttcgtcgatg ccgagcacgc gctcgatccg
     5221 atctacgccg ccaagctcgg cgtcaaggtc gccgacctgc tggtctcgca gccggacacc
     5281 ggcgagcagg cgctggagat caccgacatg ctggtgcgct cggcggcggt cgatgtcgtc
     5341 gtcgtcgatt cggtcgcggc gctgacgccg aaggccgaga tcgagggcga gatgggcgac
     5401 agccacgtcg gcctgcacgc gcggctgatg agccaggcgc tgcgcaagct caccggcaac
     5461 atcaaccgct cgaacacgct ggtgatcttc atcaaccaga tccgcctgaa gatcggcgtg
     5521 atgttcggca accccgagac caccaccggc ggcaacgcgc tgaagttcta cgcctcggtg
     5581 cgcctcgaca tccgccgcac cggctcgatc aagaagggcg acgaggtgct cggctccgag
     5641 acccgcgtca aggtggtcaa gaacaaggtc gcgccgccgt tcaagcaggc cgagttcgag
     5701 atcctctacg gcgagggcat ctcgcgcctc ggcgagatca tcgatctcgg cgtcaaggcg
     5761 gcgctgatcg acaagtccgg cgcctggtac agctacggcg gccagcgcat cggccagggc
     5821 aaggagaacg tccgccagtt cctgaaggac aacccggcga tcgccgccga actcgacgcc
     5881 aaggtgcgtg cgcattacct gagcaacccc gacgcgctgc cgaccgtgcc ggtcggtgac
     5941 gacgaggccg gcggcacggg cgatgagttc tgacgaaccc ggcgacccgg acgaccccga
     6001 ggaggccggc gcgctgctgt atcgcaccgc gctggcgctg ctggcgcgac gcgagcacac
     6061 gcggctcgaa ctcgccgcca agcttgcgcg acacggcgcc ggggccggca tcattgcggc
     6121 ggtgctcgac cggctgcagg ccgagcagct gctcagcgag cagcgtttcg ccgaggtcta
     6181 cgccgccacg cgcttcgatc gcggctacgg tccgctgcgg ctgcgcgccg agctgcgcga
     6241 acggggcgtg tcggaagcga ttgtttccga tatcctcgcg ggtttcgacg cgcagtggac
     6301 ggcgcggctg gcagccctcg cgcgccgccg cttcggtccg cagccgccga aggatgcccg
     6361 cgaacgggcg cagcgcaccg cgttcctgcg cggtcgcggt ttcaccggcg agcagatcgc
     6421 ccggctgatg cgccgcgatc ccggagacga ctgagcgccc acctccgcaa ccctgaccca
     6481 gtttgcagcc gaccatgacc agcagcgccg acctacgcct cgccttcctg gagtacttcc
     6541 gcgcccgcgg gcacgaggtc gtgccgtcga gtccgctga
//
LOCUS       DAOQJH010000034         6542 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_6591_length_6542_cov_4.324341, whole genome shotgun sequence.
ACCESSION   DAOQJH010000034 DAOQJH010000000
VERSION     DAOQJH010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 6542)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6542)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6542
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6591_length_6542_cov_4.324341"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..146)
                     /locus_tag="PLQ18_01520"
     CDS             complement(<1..146)
                     /locus_tag="PLQ18_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008452608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNE00357.1"
                     /translation="MKERLVLIGNGMAGMRTIEELQKVAPQMYDITVFGAEPHGNYNR
                     IMLSP"
     gene            complement(247..651)
                     /locus_tag="PLQ18_01525"
     CDS             complement(247..651)
                     /locus_tag="PLQ18_01525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE00358.1"
                     /translation="MNPLEEKRYHQWQFAGQLEKSQSGCGVREFRPGTHIGVMHSGSA
                     GFCASTVHLCQSRAKKRFVTALDRCESGVWRNTTKEVFPANSIWLAVFALNQGVGHGF
                     EPNRCITRMQSHGHRKVSCLHLSKKQLLVSIK"
     gene            654..2120
                     /locus_tag="PLQ18_01530"
     CDS             654..2120
                     /locus_tag="PLQ18_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005958538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015514 - nitrite efflux transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015707 - nitrite transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NarK family nitrate/nitrite MFS transporter"
                     /protein_id="HNE00359.1"
                     /translation="MASSNIKLLSFSGNVRILHLSWIAFFISFVVWFNHAPLLASIRE
                     TFQLTDGQVKALLTLNVALTIPVRILIGMLVDKFGPRIMYSVLLAVCGGLCFFFALAN
                     SYEQLALARLLLGCVGAGFVIGIRLISEWFPAKQVGFAEGIYGGWGNFGSAAAALSLP
                     VLAHAFGGPDAWRWAVGVTGVISIIYSVIFYALASDTPKGSTYFRPKKLGAMEVTSKS
                     DFWLYVFLYVPMTVALLVLTWKLSPAGFKLIDQTTTNVVYVALLALFAWNVWQVIDVN
                     RNILAGEVVPQIHRYKFKQVAILSGAYAIAFGSEIAVVSMLPLYFKDMYNLTLIQAGL
                     VGSSFAIMNLFARPAGGLISDRFGRKRTMTIVMIGITFGYFGMSFMGGMPLWAAVTVT
                     VINSFFVQAGCGAVYGIIPLIKRRLTGQIAGMSGAYGNVGSVVFLTVLSFVTPSTFFM
                     VIAASALVVLGLVQFLDEPSGQMAEIMPDGSVQMIDVT"
     gene            2220..3959
                     /locus_tag="PLQ18_01535"
     CDS             2220..3959
                     /locus_tag="PLQ18_01535"
                     /EC_number="2.7.11.-"
                     /EC_number="3.1.3.-"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008290765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-serine/threonine
                     kinase/phosphatase"
                     /protein_id="HNE00360.1"
                     /translation="MTARTSPAVLSLRIGQHSERGAKPANEDFHGMLVPEGQALQLKG
                     VAIAIADGMSSSEAARLAAEYAVKGFLNDYYATPDSWTVRKSGEKVLSALNRWLYAQS
                     TALRDPARGLVTTFSALVLKSTTAHVFHIGDSRIWQLRDQTLEPLTRDHHAFGSGGRS
                     WLNRALGIDLRLDIDYRAVPVEVGDRYLFTTDGIHEYLQPREIKRLLNEHSEDPDAAC
                     RALAAAAHAAGSPDNLTCQLICIDSLPAQDADSVFRQLAELPFPPELETGKHFEGYRI
                     EREIHASSTSQLYLAVDEQSGERVVLKTPSVNYEDDPAYLERFRHEEWVGRRLNSPHV
                     MRVIEPPRRRRHLYHVLEYLDGQTLRQWMQAHPQPTLAETRALLGQIARGLRAFHRLD
                     MLHLDLKPENIHIDREGRVRIIDFGSVKIGGIAETASPVERTDLVGTRDYTAPELLLG
                     ESATTRCDLFSFGVIAYELLTGHLPYGERLGRETDAKALAKLHYIPARLTRSDLPRWV
                     DGALEKSVRLDPRLRYTVETEFMHDLLHANTEFTGGPVPPLIERNPLGLWRTLALLSL
                     VLNLVLLFLLSRH"
     gene            complement(4814..5608)
                     /locus_tag="PLQ18_01540"
     CDS             complement(4814..5608)
                     /locus_tag="PLQ18_01540"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019021053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNE00361.1"
                     /translation="MNAYLNIDNVDMTFHTQSGTFHALRNINLKIAKGEFVSLIGHSG
                     CGKSTVLNIVAGLLNATRGGVLLENREVNRPGPDRAVVFQNHSLLPWLSVYDNVRIAV
                     DKVFGKTTSSAERDEMTRLNLKRVHMDHAIHKRPGEISGGMKQRVGIARALAMQPKVL
                     LMDEPFGALDALTRAHLQETVMELQVELGNTVIMITHDVDEAVLLSDRIVMMTNGPAA
                     TIGEVLKVDLPRPRNRLELADDVSYNHYRAEVLKFLYERQKRPDAA"
     gene            complement(5634..6455)
                     /gene="ntrB"
                     /locus_tag="PLQ18_01545"
     CDS             complement(5634..6455)
                     /gene="ntrB"
                     /locus_tag="PLQ18_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014745661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015414 - ABC-type nitrate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015706 - nitrate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate ABC transporter permease"
                     /protein_id="HNE00362.1"
                     /translation="MSTASTASASASPSRIRLKWLANVALPLLTFGVVLAVWSFISLK
                     ITSDIPAPTVAWQRAIELFSDPFYQKGPNDMGIGWQVLYSLGRVLGGFALAVLVGIPV
                     GFMMGASETVNRAVYPLVQVLRPVSPLAWLPIGLLLFKAVDPSALFVIFITSIWPVIV
                     NTAAGVKAIPQDYMNVARVLQLGKLEITRRILIPGALPHIITGMRLSLGVAWMVIVAA
                     EMLTGGIGIGFFVWDEWNNLNVASIIVAIGVIGVVGIALEAAMNLVQNRLDYTRR"
BASE COUNT         1093 a         2171 c         2141 g         1137 t
ORIGIN      
        1 ggagacagca tgatgcggtt gtaattgcca tgcggctcgg cgccgaacac cgtgatgtca
       61 tacatctgcg gcgcgacctt ctgcagttcc tcgatcgtgc gcatgcccgc catgccgttg
      121 ccgatcagga ccagtctttc tttcatgaac atttcctcgc ggaagaatct ttgacgcaga
      181 gccgattcgg cgtgcggctg tgaatgcctt agcctgagcc gccgttaagc atatgccgtg
      241 ccattttcat ttaatggata caagtaactg ttttttcgaa agatgaaggc aagaaacctt
      301 tcggtgcccg tgggattgca ttctggtaat gcaccggttc ggttcaaaac cgtgccccac
      361 gccttgattc agtgcaaaaa ccgctaacca tattgaatta gctgggaata cctctttggt
      421 ggtattacgc catacccctg attcgcaccg atcaagtgcg gtcacgaagc gtttttttgc
      481 cctgctttgg cacaaatgca ccgtgctggc gcaaaaacca gcactgccgc tgtgcattac
      541 gccaatatgc gtgccgggcc tgaattcccg aaccccgcag ccggactggc ttttttcaag
      601 ttggcctgcg aattgccatt ggtgataccg cttttcttcg agaggattca ccgatggcat
      661 cgtcgaacat caagctgctg tcgttcagcg gcaacgtgcg catcctgcac ctgagctgga
      721 tcgcgttttt catcagtttc gtggtgtggt tcaaccacgc gccgctgctc gcgtcgatcc
      781 gcgaaacatt ccagctcacg gacggccagg tgaaggcgct gctgacgctg aacgtcgcgc
      841 tgaccatccc ggtgcgcatc ctgatcggca tgctggtcga caagttcggc ccgcgcatca
      901 tgtattcggt gctgctggcg gtctgcggcg gcctgtgctt cttcttcgcg ctcgccaaca
      961 gctatgagca gctggcgctg gcgcgcctgc tgctcggctg cgtcggtgcc ggcttcgtga
     1021 tcggcatccg cctgatctcg gagtggttcc cggccaagca ggtcggcttc gccgaaggca
     1081 tctacggcgg ctggggcaac ttcggttcgg ccgcagcggc actgagcctg ccggtgctcg
     1141 cacacgcgtt cggcggcccg gatgcctggc gctgggcggt cggcgtcacc ggcgtgatct
     1201 cgatcatcta ctcggtgatc ttctacgcgc tggccagcga caccccgaaa ggctcgacct
     1261 atttccgccc gaagaagctc ggcgcgatgg aagtcaccag caagagcgac ttctggctgt
     1321 atgtattcct gtacgtgccg atgaccgtgg cgctgctggt gctgacctgg aagctgtcgc
     1381 cggccggctt caagctgatc gaccagacca cgaccaacgt cgtctacgtc gcgctgctcg
     1441 cgctgttcgc gtggaacgtc tggcaggtga ttgacgtgaa ccgcaacatc ctcgccggtg
     1501 aggtcgtgcc gcagatccac cgctacaagt tcaagcaggt cgcgatcctg tcgggtgcct
     1561 acgcgatcgc cttcggctcc gagatcgccg tggtgtcgat gctgccgctg tacttcaagg
     1621 acatgtacaa cctgacgctg atccaggccg gtctggtcgg ttcgagcttc gcgatcatga
     1681 acctgttcgc gcgcccggcc ggtggcctga tctccgaccg cttcggccgc aagcgcacga
     1741 tgaccatcgt catgatcggc atcaccttcg gctacttcgg catgagcttc atgggcggca
     1801 tgccgctgtg ggcggcggtg acggtgaccg tcatcaactc gttcttcgtg caggccggtt
     1861 gcggcgcggt ctacggcatc atcccgctga tcaagcgtcg cctgaccggc cagatcgccg
     1921 gcatgagcgg tgcctacggc aacgtcggca gcgtcgtgtt cctgaccgtg ctgtccttcg
     1981 tgacgccgtc gaccttcttc atggtcatcg ccgccagcgc gctggtcgtg ctcggcctcg
     2041 tgcagttcct cgacgagccg agcggccaga tggccgagat catgccggac ggcagcgtgc
     2101 agatgatcga cgtgacctga tccgctcacc tgcagccagg acggggtgtg cggcatcgct
     2161 gcacgccccg tcggcttgtc cgaagccgcc cttccagccg agcgcccgaa ccgaccacca
     2221 tgaccgcccg cacctcgcct gccgtcctga gcctgcgtat cggccagcat tccgaacgcg
     2281 gcgccaagcc cgccaacgag gacttccacg gcatgctggt gcccgagggc caggcgctgc
     2341 agctcaaggg cgtggcgatc gcgatcgccg acggcatgag ttcgagcgag gcggcgcggc
     2401 ttgcggccga atacgcggtc aagggcttcc tcaatgatta ctacgcgacg ccggactcct
     2461 ggacggtgcg caagtccggc gagaaggtgc tgtcggcgct gaaccgctgg ctgtatgcgc
     2521 agagcacggc gctgcgcgat ccggcgcgtg gcctggtcac gaccttcagt gcgctggtgc
     2581 tgaaatcgac gacggcgcac gtcttccaca tcggcgacag ccgcatctgg cagctgcgcg
     2641 accagaccct cgaaccgctg acccgggatc accacgcctt cggcagcggc gggcgcagct
     2701 ggctgaaccg cgcgctcggc atcgacctgc gcctcgacat cgattaccgc gccgtgccgg
     2761 tcgaggtcgg cgaccgctac ctgttcacga ccgacggcat ccacgaatac ctgcagccgc
     2821 gcgagatcaa gcgcctgctc aacgaacact ccgaggaccc ggacgctgcc tgccgggcac
     2881 tggctgccgc tgcccacgcc gccggcagcc ccgacaacct gacctgccag ctgatctgca
     2941 tcgactcgct gccggcgcag gacgccgact cggtgttccg ccagctcgcc gagctgccgt
     3001 tcccgcccga actggagacc ggcaagcact tcgagggcta ccggatcgag cgcgagattc
     3061 acgcgagcag caccagccag ctgtatctgg ccgtcgacga gcagagcggc gaacgcgtgg
     3121 tgctgaagac cccgtccgtc aactacgagg atgacccggc ctatctggag cgtttccggc
     3181 acgaggaatg ggtcggccgt cgcctcaaca gcccgcacgt gatgcgcgtg atcgagccgc
     3241 cacgccggcg ccggcacctg tatcacgtgc tcgaatacct ggacggccag acgctgcgcc
     3301 agtggatgca ggcgcacccg cagccgacgc tcgcggaaac ccgcgcgctg ctcgggcaga
     3361 tcgcccgcgg cctgcgcgcc tttcaccggc tggacatgct gcatctggac ctgaagcccg
     3421 agaacatcca catcgaccgc gagggccggg tgcgcatcat cgatttcggc tcggtgaaga
     3481 tcggcggcat cgccgagacc gcatcgccgg tcgagcgcac cgatctggtc ggcaccaggg
     3541 actacactgc gccggagctg ctgctcggcg agtcggcgac gacgcgctgc gacctgttct
     3601 cgttcggcgt catcgcctac gaactgctga ccggccacct gccctacggc gagcggctcg
     3661 gccgcgagac cgacgcgaag gcgctcgcga agctgcacta catcccggcc cggctgacgc
     3721 gcagcgatct gccgcgctgg gtcgatggcg cactcgagaa atccgtgcgc ctcgacccgc
     3781 gcctgcgcta cacggtcgag accgagttca tgcacgacct gctgcacgcg aacaccgagt
     3841 tcaccggcgg cccggtgcca ccgctgatcg agcgcaaccc gctcgggctc tggcggacgc
     3901 tggcactgct gtcgcttgtg ctgaatctgg tgctgctgtt cctgctgagc cggcactgag
     3961 ccacaaaacc cccggcggcc gccgggattc cggcccgtct ctcctgagcc gcggccggac
     4021 ctgcgcagcc aggcgccggg aaaccctgat tcgccgacga tcgaggttgc acttccggtc
     4081 gggctgccgg agccgcgtgc cgtccgcagc gactccggcc agcggcagcg cgctgccggc
     4141 acgccggctc gccgggcatc ccgccctgcc ccgcgcggca ggacggcccg agccgcaggc
     4201 gccggccctg ccggcctgcg caagcccgcg aacccgtctg cggggactgc ttccgggcgg
     4261 gcggcattcc catgcatctg caagccctgc tctcgcggga ccaccagcat cgccatcgag
     4321 cgcacggcag atacagcgcc acgggcccgg aaatgcgctc cgcagccatc cggccgcgcg
     4381 cacaggctgc ggattcgatg cctgcgtccg ggcagcgacg cttgtgccgc ttcgcgccgg
     4441 gtatccgtgc acgcctgacg ccccaggctt ccagagcctg tggttcgtga aagaggcctg
     4501 cgcagggagg gcctgcggac ggactccgtg gtctgggggc ttgtatttga ggagggtccg
     4561 aaacgaggcg tgctgaagct ggaacaaaaa tatgatggta taaaaaaatt acttgaaaaa
     4621 cgtctgtccc ccggagcata cgcaccgcgc agcgcctgcc accggcagcg cgcagggaca
     4681 gcggtgcgac cgcaactgga catgggcgga ctccggacag gcaggggccg gaggcgcccc
     4741 ggcaaggggg cgcccggtca gtaccgaaac ggatgggaac tgcgggggga acggcggcgg
     4801 gaacgcggcg cgatcaggcg gcgtcgggcc gcttctggcg ctcatacagg aacttcagca
     4861 cttcggcgcg atagtggttg tacgagacgt catcggccag ttcgaggcgg ttgcgcggac
     4921 gcggcaggtc gaccttcagc acctcgccga tggtcgcggc cggaccgttg gtcatcatga
     4981 cgatgcggtc ggacagcagc acggcctcgt cgacatcgtg cgtgatcatg atcacggtgt
     5041 tgccgagctc gacctgcagc tccatgacgg tctcctgcag gtgcgcgcgg gtcagcgcgt
     5101 cgagtgcacc gaagggctcg tccatcagca gcaccttcgg ctgcatcgcc agcgcgcggg
     5161 cgatgccgac gcgctgcttc atgccgccgg agatctcgcc cgggcgcttg tggatggcgt
     5221 ggtccatgtg cacgcgcttg aggttcaggc gcgtcatctc gtcgcgctcg gccgacgagg
     5281 tggtcttgcc gaacaccttg tcgacggcga tgcgcacgtt gtcgtagacg ctcagccacg
     5341 gcagcagcga gtggttctgg aacaccacgg cgcggtccgg ccccgggcgg ttgacctcgc
     5401 ggttttcgag cagcacgccg ccgcgggtgg cgttgagcag gccggcgacg atgttcagca
     5461 ccgtcgactt gccgcagccc gagtgaccga tcagcgacac gaactcgccc ttggcgatct
     5521 tcaggttgat gttgcgcagc gcgtggaagg tgccgctctg cgtgtggaag gtcatgtcga
     5581 cgttgtcgat gttcagatag gcgttcatgg catgggctcc gggacgcaac cgatcagcgg
     5641 cgggtgtagt cgaggcggtt ctggacgagg ttcatggcgg cttcgagcgc gatgccgacc
     5701 acaccgatca cgccgatggc gacgatgatc gaggcgacat tcaggttgtt ccactcatcc
     5761 cagacgaaga agccgatgcc gatgccaccg gtcagcatct cggcagcgac gatgaccatc
     5821 caggccacgc cgagcgacag gcgcatgccg gtgatgatgt gcggcagcgc accggggatc
     5881 aggatgcggc gggtgatctc cagcttgccg agctgcagca cgcgcgcgac gttcatgtag
     5941 tcctgcggga tggccttgac gccggccgcg gtgttgacga tcaccggcca gatgctggtg
     6001 atgaagatca cgaacagcgc cgacggatcg acggccttga acagcagcag gccgatcggc
     6061 agccaggcga gcggactgac cgggcgcagc acctgcacca gcgggtacac ggcgcggttg
     6121 acggtctcgc tcgcgcccat catgaagccg accggaatgc ccaccagcac cgccagcgcg
     6181 aagccgccga gcacgcgccc gagcgagtac agcacctgcc agccgatgcc catgtcgttc
     6241 ggacccttct ggtagaacgg gtcgctgaac agctcgatgg cacgctgcca ggccaccgtc
     6301 ggcgccggga tgtcgctggt gatcttcagg ctgatgaagg accacacggc cagcacgacc
     6361 ccgaaggtca gcagcggcag cgcgacgttg gcgagccact tgagccggat gcgcgagggg
     6421 gaagcggagg cggaagcggt actggctgtg ctcatcggac ggttctcggt cgcggcgccg
     6481 gccgtggggg ccggggagcc tgccgcgtca ttcccggact ggaccggcgt tgcggcggat
     6541 tc
//
LOCUS       DAOQJH010000035         6534 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_6602_length_6534_cov_4.341874, whole genome shotgun sequence.
ACCESSION   DAOQJH010000035 DAOQJH010000000
VERSION     DAOQJH010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 6534)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6534)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6534
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6602_length_6534_cov_4.341874"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1800)
                     /locus_tag="PLQ18_01550"
     CDS             complement(<1..1800)
                     /locus_tag="PLQ18_01550"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNE00363.1"
                     /translation="MIRLPLAHKIAASVLPTVLVTVLGLGLWTYRTAYETARERIHVA
                     LGYVADRYLHDRVETIWARSWEAIDASQRARALSEAADIVPATGAGLQIVVLDEQDRV
                     LYLTGTGFAGAHADWARMAREAAAGQGTLGSSDGEWLYARRDFAPWGWRILCLMPEAS
                     VTEMLGGIRRATLTVAAVAGFVVVLLLLWLSGEVLLKPIGQLRAAAARIARSPTRLRI
                     GVPDGDELGELARAMEQMSSALAEQIDARSTAERSLERVKNLYAALSGANRAIVRVHE
                     RTELFRMLCRIAAEHGRFRLAWVGVPDEYGEMCPVASAGPASDILDGIRISIDEHLPE
                     GQGTMGRAWRSGGWAVENDFLGASVLRPWHRAARQHAIYAVAAFALREEGRTIACLSV
                     YAGERDFFRDDLIELLKDLATDVSFALDGIARAGERDRAEVARREAEERWHFALEGSG
                     DGVWDYDIPAGRIFASRRCMELLGHGDGDGVSDGYLEWEHLLHPEDRVPARLAIDAHL
                     RGETERFARDQRLLAADGRWRWFRTSGKVMRRDAEGRPLRMLGTLHDITAHKEAEARI
                     LWLGHRDALTGLLNRSLLADRLEQALVHARRTGG"
     gene            complement(1924..3309)
                     /gene="hemN"
                     /locus_tag="PLQ18_01555"
     CDS             complement(1924..3309)
                     /gene="hemN"
                     /locus_tag="PLQ18_01555"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020562787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HNE00364.1"
                     /translation="MESQIVIDERLIAKYDRAGPRYTSYPTAPQFHERFTAAHYRELA
                     LASNAGGRPLSLYFHIPFCDTVCFYCACNKIITKNRRRAGPYLERLLREIELQGALFD
                     RSRPVVQLHWGGGTPTFLSEDEMRTLMEATRAHFTLLGDERGEYSIEIDPREADAGKI
                     ALLRRLGFNRISMGVQDFDPAVQLAVNRIQPKAVTLAAIDAARAEGFRSISLDLIYGL
                     PLQTVTSFARTLDEILELDPDRLSVFNYAHLPELFKTQKQIDAAALPSPAEKLEILRL
                     VIARLTAAGYVYIGMDHFAKPDDELAVAQRAGTLYRNFQGYSTHADCDLVAMGATSIG
                     MVGDSYSQNVKTLDAWYAHLDAGELPVYRGVHLDADDRLRRAVITQLLCNFALDYAPV
                     EAAHGIVFAEYFATELAELAGMAADGLLTLDRHGIRVAPAGRLLIRNICMVFDQYLRA
                     AGTQRRYSRVI"
     gene            complement(3407..4327)
                     /gene="prmB"
                     /locus_tag="PLQ18_01560"
     CDS             complement(3407..4327)
                     /gene="prmB"
                     /locus_tag="PLQ18_01560"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011628615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HNE00365.1"
                     /translation="MTDLSLQSHLHTVRDFIRWSASRMTAEGVYFGHGTSNAIDEAAA
                     LVLHALHLPPDLHAAYFDANLVPDEKRAILNLLDQRVGQRIPLPYLTQRAWFMGLPFF
                     VDRRVLIPRSPLAELIERQFAPWVQAEKVSRILDLCTGSGCIGIACAHAFPEAEIDLA
                     DISTEALDVAAINVADHGVDDRVELIRSDLFSALAGRRYGLILSNPPYVGHDEMTALP
                     PEYAHEPRLALESGEDGLDAPLAILKQAREFLEDDGVLVVEVGNAQWALRERLPELPF
                     LWLEFDRGGEGVFLLSAAELDEHADAIAAA"
     gene            4469..5110
                     /locus_tag="PLQ18_01565"
     CDS             4469..5110
                     /locus_tag="PLQ18_01565"
                     /inference="COORDINATES: protein motif:HMM:NF014667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HNE00366.1"
                     /translation="MMRTRPLLLAFAGIAALAAGILLGDRLVGPPPDPLPSIHGTVIA
                     PPQPLPAFQLRDEHDTPFGPERFRGRWTLIYFGYTYCPDVCPTELTTLAELEKRLAAQ
                     GRGGDTDYWLITVDPERDTVQRLREYVQFFSKRFHAASGDTANLAALMNPLGVIALRP
                     PGQPDTGYTIDHSSTLTLIDPQGRLHAIFSPPNEVEHLLPELLALQARYAARP"
     gene            5141..6028
                     /gene="asd"
                     /locus_tag="PLQ18_01570"
     CDS             5141..6028
                     /gene="asd"
                     /locus_tag="PLQ18_01570"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229092.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HNE00367.1"
                     /translation="MTDETPTLAERLRNSPQYWLPAAAITALTHRITRVRAPWLRKPL
                     IRWFIDAYRVNMAEAAEPDPEAYEHFNAFFTRALAAGARPIVAGEDRLCCPVDGAVSQ
                     AGALAGERVLQAKGHDYSLTALLGGEPADSAPFAGGCFATLYLSPRDYHRIHMPVDGR
                     LRTMIHVPGRLFSVSPLTTRCIPGLFARNERVVTLWDTAAGPMALVLVGAINVASMET
                     VWAGTITPPLGGAIRRWQYPAEGEGAVHLAKGDELGRFNMGSTVILAFAPGAVGLDAS
                     IRAGAPVRLGQTLAQRLVG"
     gene            6025..>6534
                     /locus_tag="PLQ18_01575"
     CDS             6025..>6534
                     /locus_tag="PLQ18_01575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE00368.1"
                     /translation="MTAARSAAATLLMLALGGCALAPAGEDQASNVGGVTVAPLMPAQ
                     ASPAASLAAFSPKYVTAVPHPRPSLSVPAVAAPLHLGYRSLAGGGEIGFHGRLPAPRF
                     GWFTRSQLELGAAVQGREQRLDARAAARLRLTHEPGNQRWRAGLELGSALRSRPALRA
                     HWTRRHDAGA"
BASE COUNT          935 a         2338 c         2322 g          939 t
ORIGIN      
        1 cccgccggtg cgccgcgcat gcaccagtgc ctgttcgagg cggtcggcga gcaggctgcg
       61 gttgagcagg ccggtcagcg cgtcgcggtg cccgagccac agaatgcggg cctcggcctc
      121 cttgtgcgcg gtgatgtcgt gcagcgtgcc gagcatccgg agcggccggc cttcggcgtc
      181 gcggcgcatg accttgcccg aggtccggaa ccagcgccag cggccgtcgg ccgcgagcag
      241 gcgctggtcg cgcgcgaagc gctcggtctc gccgcgcagg tgcgcatcga tcgccaggcg
      301 cgccggcacg cggtcctcgg ggtgcagcag gtgctcccac tccagatagc cgtcggaaac
      361 gccgtcgccg tcgccgtggc cgagcagttc catgcagcgg cgcgacgcga agatgcgccc
      421 ggccggaatg tcgtagtccc agacgccgtc gccgctgcct tcgagcgcga agtgccagcg
      481 ctcctcggcc tcgcgccgcg ccacctcggc gcggtcgcgc tcgccggcgc gggcgatgcc
      541 gtcgagcgcg aaggacacgt cggtggcgag atccttcagc agctcgatca ggtcgtcgcg
      601 gaagaagtcg cgctcgccgg cgtagacgct caggcaggcg atcgtgcggc cttcctcgcg
      661 cagcgcgaag gccgcgaccg cgtagatcgc gtgctggcgc gcggcgcgat gccagggccg
      721 cagcaccgaa gcgccgagga agtcgttctc gaccgcccag ccgccgctgc gccaggcccg
      781 gcccatcgtg ccctggccct cgggcaggtg ctcgtcgatc gagatgcgga tgccgtcgag
      841 gatgtcgctc gccggaccgg cgctcgcgac cgggcacatc tcgccgtatt cgtcgggcac
      901 accgacccag gccagccgga agcgcccgtg ctcggcggcg atgcggcaga gcatgcggaa
      961 cagctcggtg cgctcatgca cgcgcacgat cgcgcggttg gcgccggaga gcgccgcgta
     1021 caggttcttg acgcgctcca ggctgcgctc ggccgtgctg cgcgcgtcga tctgctcggc
     1081 gagggccgag gacatctgtt ccatcgcgcg cgccagttcg ccgagttcgt cgccgtcggg
     1141 cacgccgatg cgcagccgcg tcgggctgcg ggcgatgcgc gcggccgcgg cgcgcagctg
     1201 gccgatgggc ttgagcagca cctcgccgga gagccacagc agcagcagca cgacgacgaa
     1261 gccggcgacg gcggcgacgg tcagcgtcgc gcggcggatg ccgccgagca tctcggtgac
     1321 gctcgcctcc ggcatcaggc agaggatgcg ccagccccag ggcgcgaagt cgcggcgcgc
     1381 atacagccat tcgccgtcgg acgagccgag cgtgccctga ccggcggcgg cctcgcgcgc
     1441 catgcgtgcc cagtcggcgt gcgccccggc gaagccggtg ccggtcaggt acagcacgcg
     1501 gtcctgctca tcgagcacga cgatctgcag gccggcgccg gtggccggca cgatgtcggc
     1561 ggcctcgctg agcgcccggg cgcgctggct ggcgtcgatc gcctcccagc tgcgggccca
     1621 gatcgtctcg actcggtcat gcaggtagcg atcggcgacg tagccgagcg cgacgtggat
     1681 gcgctcgcgc gcggtctcgt aggcggtgcg gtaggtccag agcccgaggc cgagcaccgt
     1741 gaccagcacg gtcggcagca ccgaggccgc gatcttgtgc gcgagcggca ggcggatcac
     1801 ggcggcgatc cgccgtgcgg ctgcggatgg aatgtcggca aggctccctc tcccctgcgg
     1861 cgctctggcg gcgcctgcac ggccgtcccg agtcaggcgg gtcaaccgga tcagacgacc
     1921 cgatcagatg acgcgagaat accggcgctg cgtacccgct gcacgcaggt attgatcgaa
     1981 aaccatgcag atgttgcgga tcagcaggcg gccggccggc gcgacgcgga tgccgtgccg
     2041 atcgagcgtg agcaggccgt cggcggccat gccggcaagc tcggcgagct cggtcgcgaa
     2101 atactccgcg aagacgatgc cgtgggcggc ctcgaccggc gcgtaatcga gcgcgaagtt
     2161 gcagagcagc tgcgtgatca cggcgcggcg caggcggtca tcggcatcga ggtgcacgcc
     2221 gcggtagacc ggcagctcgc cggcatcgag gtgcgcgtac caggcgtcca gggtcttgac
     2281 gttctggctg taggaatcgc cgaccatgcc gatcgaggtc gcgcccatcg ccaccaggtc
     2341 gcagtcggcg tgcgtgctgt agccctggaa gttgcggtac agcgtgccgg cgcgctgcgc
     2401 gacggcgagt tcgtcgtcgg gcttcgcgaa gtgatccatg ccgatgtaca cgtagccggc
     2461 cgcggtcagg cgggcgatca cgagcctgag gatttcgagc ttctcggccg gcgacggcag
     2521 cgccgcggca tcgatctgct tctgggtctt gaacagctcc ggcaggtgcg cgtagttgaa
     2581 taccgacagc cggtccggat cgagttcgag gatctcgtcg agcgtgcgcg cgaagctcgt
     2641 gaccgtctgc agcggcaggc cgtagatcag atcgaggctg atcgagcgga aaccctcggc
     2701 gcgcgccgcg tcgatcgccg cgagcgtcac cgccttcggc tggatgcggt tgacggcaag
     2761 ctgcacggcc gggtcgaagt cctgcacgcc catgctgatg cggttgaagc cgagccggcg
     2821 cagcagcgcg atcttgccgg cgtcggcctc gcgcgggtcg atctcgatcg agtactcgcc
     2881 gcgctcatca ccgagcagcg tgaagtgtgc gcgcgtcgcc tccatcagcg tgcgcatctc
     2941 gtcttcggat aaaaaggtcg gcgtgccgcc gccccagtgc agttgcacga ccgggcgcga
     3001 gcgatcgaac agcgcgccct gcagctcgat ctcgcgcagc agccgttcga gatacggccc
     3061 ggcgcggcgg cggttcttcg tgatgatctt gttgcaggcg cagtagaagc agaccgtgtc
     3121 gcagaacggg atgtggaagt acagcgacag cggccggccg ccggcattgc tcgcgagcgc
     3181 gagctcccgg tagtgagcgg ccgtgaagcg ctcgtggaac tgcggcgcgg tcgggtagga
     3241 ggtgtagcgc ggcccggcgc ggtcgtactt cgcgatcagg cgctcgtcga tgacgatctg
     3301 ggattccatc ggtcggtccg gaaagaggct cgtgcggggt gaagcggcgc agcctggcgc
     3361 agcgcgcgac cggcggcctt gatccactgc cgggcggcgg gtgttttcag gccgcggcga
     3421 tcgcgtcggc gtgctcgtcg agttcggccg cgctcagcag gaacacgccc tcgccgccac
     3481 gatcgaattc gagccacagg aacggcagct ccggcaggcg ctcgcgcagc gcccactggg
     3541 cgttgccgac ctcgaccacc agcacgccgt cgtcttcgag gaactcgcgc gcctgcttca
     3601 ggatcgcgag cggcgcatcg aggccgtcct cgcccgattc gagcgcaagc cgcggctcgt
     3661 gcgcgtattc gggcggcagc gccgtcatct cgtcgtggcc gacatagggc gggttgctca
     3721 ggatcagccc gtagcgccgc ccggccagcg ccgagaacag atccgagcgg atcagctcga
     3781 cgcgatcgtc gacgccgtga tccgcgacgt tgatcgccgc cacatcgagc gcctcggtcg
     3841 agatgtcggc gagatcgatc tccgcctccg gaaaggcatg cgcgcaggcg atgccgatgc
     3901 agccgctgcc ggtgcagaga tcgaggatgc gcgagacttt ttcagcctgc acccagggcg
     3961 cgaactggcg ctcgatcagc tcggcgagcg gcgagcgcgg aatcagcacg cgccggtcga
     4021 cgaagaacgg caggcccatg aaccaggcgc gctgcgtcag gtacggcagc ggaatgcgct
     4081 ggccgacgcg ctggtcgagc aggttcagga tcgcgcgctt ctcgtcgggc acgaggttgg
     4141 cgtcgaaata cgcggcatgc aggtccggcg gcaggtgcag cgcatgcagc accagcgccg
     4201 ccgcctcgtc gatcgcgttg ctggtcccgt ggccgaagta cacgccttcg gccgtcatgc
     4261 ggctcgcact ccagcggatg aaatcgcgca cggtatgcag gtgggattgc aggctgaggt
     4321 cggtcatcgc tgcgcactcc gcggaaaagg ccgcgactat atcaccgcct cgtggcaccg
     4381 gccggacgcg cacgaccatg cgctacactg cgccgcttcc tgccgacccg gccgccgcgc
     4441 ctgcccgccc tccggagtga tgcccgacat gatgcgcacg cgcccgctgc tccttgcctt
     4501 cgccggcatt gccgccctcg ccgccggcat cctgctcggt gatcgtctcg tcggcccgcc
     4561 gcccgatccg ctgccgagca tccacggcac cgtgatcgcg ccgccgcagc cgctgccggc
     4621 cttccagctg cgcgacgaac acgacacgcc cttcggaccg gaacgcttcc gcggtcgctg
     4681 gacgctgatc tacttcggct acacgtattg ccccgatgtc tgcccgaccg agctgaccac
     4741 gctcgccgag ctcgaaaagc ggctggcggc gcagggcagg ggcggcgaca ccgactactg
     4801 gctgatcacg gtcgatcccg agcgcgacac cgtccagcgc ctgcgcgagt acgtgcagtt
     4861 cttcagcaag cgcttccacg ccgccagcgg cgacaccgcc aacctcgcgg cgctgatgaa
     4921 tccgctcggc gtcatcgcgc tgcgtccgcc gggacagccg gacaccggct acacgatcga
     4981 tcattcctcg acgctgacgc tgatcgaccc gcagggccgg ctgcacgcga tcttctcgcc
     5041 gccgaacgag gtcgagcacc tgctgcccga actgctggcg ctgcaggccc gctacgccgc
     5101 ccgtccctga acgcccgccg cctccggatc cgccgccccg atgaccgacg aaaccccgac
     5161 gctcgccgag cgcctgcgca acagcccgca gtactggctg ccggccgccg cgatcacggc
     5221 gctgacccac cgcatcacgc gcgtgcgcgc gccgtggctg cgcaagccgc tgatccgctg
     5281 gttcatcgac gcctaccgcg tcaacatggc cgaggccgcc gagcccgatc ccgaggccta
     5341 cgagcatttc aacgccttct tcacgcgcgc gctggccgcc ggcgcccgcc cgatcgtcgc
     5401 cggcgaggac cggctctgct gtccggtcga tggagccgtc agccaggccg gtgcgctcgc
     5461 cggcgagcgc gtgctgcagg ccaagggtca tgactattcg ctgacggcgc tgctcggtgg
     5521 cgaacctgcc gacagcgcgc cgttcgccgg cggctgcttc gcgacgctgt acctcagtcc
     5581 gcgcgattac caccgcatcc acatgccggt cgacggccgc ctgcgcacga tgatccacgt
     5641 gccgggccgg ctgttctcgg tgtcgccgct gaccacgcgc tgcatccccg gtctgttcgc
     5701 gcgcaacgag cgcgtcgtga cgctctggga caccgccgcc ggcccgatgg cgctggtgct
     5761 ggtcggggcg atcaacgtcg cgtcgatgga aaccgtctgg gccggcacga tcacgccgcc
     5821 gctcggaggc gcgatccgcc gctggcagta tccggccgaa ggcgaaggcg cagtgcatct
     5881 ggcgaagggc gatgaactcg ggcgcttcaa catgggctcg accgtgatcc tcgccttcgc
     5941 gcccggcgcc gtcggcctgg acgcctcgat ccgtgccggc gcgccggtgc gcctcggtca
     6001 gacactggcg cagcggctgg tcggatgacg gccgcccgaa gcgccgctgc gacgctgctg
     6061 atgctggcgc tcggcggctg cgcactggcc ccggccgggg aagatcaggc gtcgaacgtc
     6121 ggcggcgtca ccgtggcacc gctgatgccg gcgcaggcga gtccggccgc ctccctcgcg
     6181 gcgttcagcc cgaagtacgt caccgccgtg ccgcatcccc gcccgtccct gtccgtgccg
     6241 gccgtggccg cgccgctgca cttggggtat cgcagcctcg ccggcggagg ggagatcggt
     6301 ttccacggcc ggctgccggc gccgcgcttc ggctggttca cccgctcgca actcgaactc
     6361 ggagccgccg tgcagggccg cgagcaacgg ctcgatgccc gtgccgctgc ccggctgcgc
     6421 ctgacgcacg agccgggcaa ccagcgctgg cgcgccggac ttgaactcgg cagcgcgctg
     6481 cgcagccgcc cggcgctgcg ggcacactgg acccggcgcc acgacgccgg tgcg
//
LOCUS       DAOQJH010000036         6473 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_6684_length_6473_cov_3.912745, whole genome shotgun sequence.
ACCESSION   DAOQJH010000036 DAOQJH010000000
VERSION     DAOQJH010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 6473)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6473)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6473
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6684_length_6473_cov_3.912745"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(65..793)
                     /locus_tag="PLQ18_01580"
     CDS             complement(65..793)
                     /locus_tag="PLQ18_01580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE00369.1"
                     /translation="MLHTMKALLASIAKVMPLWLFWLLAGLTALSCLVALVLIFRLLM
                     RRARRGPVMIVQGPETLPDAPGGSLRCYRLDAVEALAGSGILQLLVSPSGVFAISGLA
                     VSGRLSGQARGESWRLAHRGEPRQSLPNPLLACRAQLPKLAQVLGVAPDKLHPCVLVA
                     ATARWSSSSECPVGVYFSAEELIGDVRQRLNVVFSAPERQALLARLDGLLKPSELAEP
                     VTAAPAKTARHWLRRRSSSPVQAG"
     gene            complement(960..1631)
                     /locus_tag="PLQ18_01585"
     CDS             complement(960..1631)
                     /locus_tag="PLQ18_01585"
                     /inference="COORDINATES: protein motif:HMM:NF019976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease-related domain-containing protein"
                     /protein_id="HNE00370.1"
                     /translation="MSPQSPSYLTQLMPLLVSWLTVLLPMLCALLPAVLFFRGRQQGR
                     NGSLGRMLQRKLRPADGFHVYHDLEFENDYGECVRIEHLVLGSSGVYPIISTRLSGEV
                     LADIDASVPWRRMLHGREQPIPNPLARVHGAADLVSRSLGVAPACVHPLLVVTGVTRL
                     AMPPSRVAVFLSPKSACEAVHDRLALSFHDEQLQALRARLDTAQRTERPLSAPLPQAS
                     RPSAL"
     gene            1797..2663
                     /locus_tag="PLQ18_01590"
     CDS             1797..2663
                     /locus_tag="PLQ18_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HNE00371.1"
                     /translation="MPVQLLVPPRPTDLGGFSVRRLLPHRSCRAVGPFVFFDHMGPAD
                     FAPGHGIDVRPHPHIGLATVTWLFDGEILHRDSLGSVQPIRPGALNWMTAGRGIVHSE
                     RTGPDTRIRGGRLHGIQAWVALPEADEECEPGFVHYPAEALPQFTRNGVELILIAGTA
                     FGERSPVEVAMPTFYLEARLAAGARLMLEDEHPERAVYVAEGAARVDVRALQAGDLAV
                     LAPGGTVAIEADGPCRLMLLGGAPLDGPRTIWWNFVSSRPQRIEQAKADWRANRFAHV
                     PGESEFIPLPED"
     gene            2819..3241
                     /gene="trxC"
                     /locus_tag="PLQ18_01595"
     CDS             2819..3241
                     /gene="trxC"
                     /locus_tag="PLQ18_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HNE00372.1"
                     /translation="MELNCPHCQRRNRLPPERLPELPNCGACGAPLLEGGVLELDAGS
                     FDALIAHAPRPVLVDFWAPWCSPCRQFAPTYLATAKILSERYVFAKLDTEAEPEIAAR
                     TGIRSIPTLVLFHEGHELSRRSGALPGPELRRWLLMQD"
     gene            3559..3882
                     /locus_tag="PLQ18_01600"
     CDS             3559..3882
                     /locus_tag="PLQ18_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HNE00373.1"
                     /translation="MPKPTRHVLVCTQMRPPNHPRGACAHRDAGAVMNRFAEAFQARG
                     LWGSYLLSQTSCIGPCDAGTNVLVYPDNVLYSGVGTADVDEIIEAHVLGGQPVERLLA
                     APQNW"
     gene            complement(4019..4489)
                     /locus_tag="PLQ18_01605"
     CDS             complement(4019..4489)
                     /locus_tag="PLQ18_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010628073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase"
                     /protein_id="HNE00374.1"
                     /translation="MQTLTALDARAESTVHGVRWLKLAVVYLLAGVVLGIAMGASHDF
                     TLRPVHAHLNLLGWTTLGLAGLIYAVYPQAATTRCARVHFWLFALALPVMMLALTGLL
                     LGQGAALPALVASEFAILGGVLAFALNVFLNLMPRRAASASCLPADCVPAAGRA"
     gene            complement(4755..6119)
                     /locus_tag="PLQ18_01610"
     CDS             complement(4755..6119)
                     /locus_tag="PLQ18_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019143106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNE00375.1"
                     /translation="MSGVDAIRDCLDGAIPAILATCSAEGEPNVAYLSQVEYVDDAHV
                     ALSYQFFNRTRQNVLAHPVARLIVTHPRTGARHRLLLHYLRTETGGALFERMKAKLAG
                     IASHTGMSGVFRLLGSDVYRVLSIERVPGPALAAPAPRRSPLAVLRACSESLNAYTEL
                     DELLAGALAVFERELDVRHWLLLMLDEAGERLYTLASRGYGASGIGAEIALGEGLIGV
                     AARVGTPVRIGHATAEYGYGRAIRAAAADGGLLDALETEIPLPGLADSRSQIAVPIRA
                     CGRLLGVLYAESTEDLRFGYDDEDLLVACAALLGTAMRLLQLEAHAQEGAAVPAPPAA
                     GPAPIADAAPLTVRHYAANDSVFLDGQYLIKGVAGSILWTLLGDYVALGRTSFSNREL
                     RVDPRIRLPGLSDNLEARLILLSRRLAEHEACVRLARTGRGRLELQVGRPLQLVEIAA
                     AEAG"
     gene            complement(6116..>6473)
                     /locus_tag="PLQ18_01615"
     CDS             complement(6116..>6473)
                     /locus_tag="PLQ18_01615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE00376.1"
                     /translation="WLAASASAALLADVAANATLAMVCSLPATHRTVQLKTRDARIGA
                     LEAGDRDCVARYRAAFVGGLEALGYPAAVMSMLVAATDEDLAAIDFTPHAAFVQTPGP
                     AAGALLVAAGGEGHAP"
BASE COUNT         1013 a         2289 c         2313 g          858 t
ORIGIN      
        1 ccacgatgcc tcgaccgccg ggctgatcgc ccgcatccgc gccttgcgca cgcagtaggc
       61 aggttcagcc ggcctgcacc ggcgaggacg accggcgccg cagccagtgg cgagcggtct
      121 tcgccggcgc ggccgtgacc ggctcggcga gttccgaggg cttcagcagc ccgtcgagcc
      181 gggcgagcag cgcctggcgc tccggagccg agaacaccac attcaggcgc tggcgcacgt
      241 cgccgatcag ttcctcagcc gagaaataca cgccgaccgg gcactcggat gacgacgacc
      301 agcgcgccgt cgccgccacc agcacgcagg gatgcagctt gtcgggcgcg acgccgagca
      361 cctgggcgag cttcggcagc tgcgcgcggc acgcgagcag cggattcggc aaggactggc
      421 gcggctcgcc gcgatgcgcg aggcgccagg actccccgcg tgcctggccg ctgagccggc
      481 cgctgaccgc cagcccgctg atcgcgaaca cgccggacgg cgacaccagc agctgcagga
      541 tgccgctgcc ggccagcgcc tcgacggcat cgagccggta gcagcgcagc gatccgcccg
      601 gggcatccgg cagggtttcg ggcccctgga cgatcatgac cggcccgcgg cgcgcacggc
      661 gcatcagcag ccggaagatc agcaccagcg ccacgagaca gctcagcgca gtcagccccg
      721 cgagcaacca gaacagccag agcggcatca ccttggcgat ggatgccagc aacgccttca
      781 tcgtatgcag catgtacaca gtcctttgcc ggggcggtcg gggaaatcac aacggccgga
      841 gccgggtcag accggcgggg cgaagcagaa tttcagcata ccggcaaacg caaggcgccg
      901 gacgcccgca agcggacgtc cggcgccgga gcagccgggc ggatcagcgg atcagcggat
      961 cagagcgcgg acgggcggct cgcctgcggc agcggcgccg acagcggccg ctcggtgcgc
     1021 tgggcggtgt cgagccgggc gcgcagcgcc tgcagctgtt cgtcgtgaaa gctcagggcc
     1081 agccggtcgt gcacggcttc gcaggcggac ttcggcgaca ggaacacggc aacgcggctc
     1141 ggcggcatcg ccaggcgcgt gacgccggtg acgaccagca gcggatgcac gcaggccggc
     1201 gcgacgccga ggctgcggct gaccagatcg gcggcaccgt gcacgcgcgc gagcggattc
     1261 gggatcggct gctcgcgccc gtgcagcatg cgccgccacg gcacggaggc atcgatgtcg
     1321 gcgagaacct cgccgctcag ccgggtgctg atgatcggat agaccccgct cgatccgagc
     1381 acgaggtgct cgatgcgcac gcactcgccg taatcgttct cgaattcgag atcgtgatag
     1441 acgtggaagc catcagccgg ccggagcttg cgctgcagca tgcgtccgag cgaaccgttg
     1501 cgcccctgct ggcggccccg gaagaagagc acggccggca gcagggcgca gagcatgggc
     1561 agcagcacgg tcagccagga aaccagcagc ggcatcagct gcgtcaggta cgacgggctc
     1621 tgcggggaca tcgagggcaa atcctcagct gcagcggggt tgttccggaa acgcgcggga
     1681 ttgtcatatg ccgcgccttg ccgccgggcc tgtcgactga aattgtcgca atctttaacg
     1741 caggagccgc gcatgtccga tgcttgagca cccttctccc tgcggagttc cccgccatgc
     1801 cagtgcagct gctcgttccg ccccgaccaa ccgatctcgg cggcttcagc gtccgccggc
     1861 tgctgccgca ccgcagctgc cgggcggtcg gccccttcgt gttcttcgat cacatgggac
     1921 cggccgactt cgcccccggt cacggcatcg acgtgcgtcc gcatccgcac atcgggctcg
     1981 cgaccgtcac ctggctgttc gacggcgaga tcctgcaccg tgacagcctc ggctccgtgc
     2041 agccgatccg ccccggggcg ctgaactgga tgaccgccgg gcgcggcatc gtgcattccg
     2101 agcgcaccgg tccggacacc cgcatccgtg gcggccgcct gcacggcatc caggcctggg
     2161 tggcgctgcc ggaggccgac gaggaatgcg aaccgggctt cgtgcattac ccggccgagg
     2221 cgctgccgca gttcacgcgc aacggcgtcg agctgatcct gatcgccggc acggccttcg
     2281 gcgagcgctc gccggtcgag gtcgcgatgc cgaccttcta cctcgaagcc cgcctcgctg
     2341 ccggtgcccg gctgatgctc gaggacgagc acccggagcg cgccgtctac gtcgccgagg
     2401 gcgcggcgcg ggtggatgtc cgcgcactgc aggccggcga tctggcggtg ctcgcccccg
     2461 gcggcacggt cgcgatcgag gccgacgggc cgtgccggct gatgctgctc ggcggggcac
     2521 cgctcgacgg accgcgcacg atctggtgga acttcgtttc cagccgcccg cagcgcatcg
     2581 agcaggccaa ggccgactgg cgcgcgaacc gcttcgccca cgtgcccggc gagtccgagt
     2641 tcatcccgct gcccgaggat tgagccgccc gcaggccggg cagacgcccc ccgcttcgcc
     2701 cccgcccggc gttgtcgccc gccccgcggg gcgtcaggcg atcggcaggc cattgaaatc
     2761 aggccggtcg ccagcagatt catggtctcc gcccacgatc ggcccaggat tcccggacat
     2821 ggaactgaac tgcccgcact gccagagacg caatcgcctg ccccccgaac gcctgcccga
     2881 actgccgaac tgcggtgcct gcggggcacc gctgctcgaa ggcggcgtgc tcgaactcga
     2941 tgccggcagc ttcgacgccc tgatcgccca cgccccgcgc ccggtactgg tggatttctg
     3001 ggcaccgtgg tgcagcccgt gccgccagtt cgcgccgacc tacctggcaa cggcgaagat
     3061 actgtccgag cgttacgtgt tcgcgaagct cgacaccgaa gccgaaccgg aaatcgccgc
     3121 caggaccggc atccgcagca ttccgacgct ggtgctgttt cacgaaggcc acgaactgtc
     3181 gcgccgcagc ggcgcgctgc cgggcccgga gctgcgccgc tggctgctga tgcaggactg
     3241 aagtcccgat ccgccgctcc ggcatcttcg ggccgaccgg gggaatcgag gccgctgacc
     3301 ggggtcggcg ggcggaccgc agtgggggtc cggccagtcc cggcccggtg cttcgcgggt
     3361 ggcggcggac gcccgatccg gacaccgggt ctggccggct gtccggaccc cgacagcacc
     3421 gaccgtttcc ggacgccggt gcgacattga cccgcttgca ccaggccact gacaaataac
     3481 gagtatcaac gaatatcagg gaatggcacc gtttgtgctg cgctgcaata cataccgaca
     3541 acaggagatc gcagcgccat gccgaaaccg acccgccacg tcctcgtctg cacgcagatg
     3601 cgcccgccga atcacccgcg cggtgcctgc gcgcatcgtg atgccggtgc cgtgatgaac
     3661 cgcttcgccg aagccttcca ggcccgcggg ctgtggggca gctacctgct gtcgcagacc
     3721 agctgcatcg gtccctgcga tgccggcacg aacgtgctcg tctacccgga caacgtcctg
     3781 tacagcggcg tcggcaccgc cgatgtcgac gagatcatcg aagcccacgt gctcggtggc
     3841 cagccggtcg agcgcctgct cgccgcgccg cagaactggt aagccccgcc tgcagacgac
     3901 cggccgctcc ccgtgccctg cgctgctgcg acgggctccg ggtgagcggc cggatttttt
     3961 cgggattttc ccgggctcct tcaggggtga gcggccgggc gggaaagcgg ccggacgttc
     4021 aggcgcgacc ggcggccggc acgcagtcgg ccggcaggca gctggcactg gccgcacgac
     4081 gcggcatcag gttcaggaac acgttcagcg caaaggcgag cacgccgccg aggatcgcga
     4141 actccgaggc caccagcgcc ggcagggccg cgccctggcc gagcagcagg ccggtcagcg
     4201 ccagcatcat gaccggcagc gccagcgcga acagccagaa gtgcacgcgc gcacagcgcg
     4261 tcgtcgccgc ctgcggatag accgcgtaga tcagcccggc cagccccagc gtcgtccagc
     4321 cgagcaggtt caggtgcgca tgcaccggac gcagcgtgaa gtcgtggctg gcacccatgg
     4381 cgatgccgag cacgacgccg gcgagcaggt agacgacggc gagcttcagc cagcgcacgc
     4441 cgtggacggt ggattcggcg cgggcatcga gggcggtgag ggtctgcatg atggaactcc
     4501 ggatcggaca ggttcgggaa aggcctggaa aggtctggat caggtttcgg gaggcatccg
     4561 tgcccgcgca ccggctcgcg gaccgggcgg ctgcggatac ggcctggtcg gcaagtcccg
     4621 gtacggagcc ggttttctgc ttcgggccgc agtgcgggac ggattctggc tgcaggggcc
     4681 gggcgataca tccggcccgc gccgtagtgc agcgaatctt cggattcctt taggaaactt
     4741 ttaggatcgg ccgctcagcc ggcctcggcg gcggcaatct cgaccagctg cagcgggcgt
     4801 ccgacctgca gttcgagccg gccgcggccg gtgcgcgcca gccgcacgca ggcctcgtgc
     4861 tcggcgagcc ggcgcgacag caggatcagc cgggcttcga ggttgtcgct caggcccggc
     4921 agccggatgc gcgggtcgac gcgcagttca cggttgctga agctcgtgcg cccgagcgcg
     4981 acgtagtcgc cgagcagcgt ccagaggatc gaaccggcca cgcccttgat caggtactgg
     5041 ccgtcgagga acacgctgtc gttggccgcg taatgacgca cggtcagcgg cgctgcatcc
     5101 gcaatgggcg ccgggccggc agcgggcggg gccgggacgg cggcgccctc ctgggcatgg
     5161 gcttcgagct gcagcaggcg catcgcggtg ccgagcaggg ccgcgcaggc gaccagcagg
     5221 tcctcgtcgt cgtagccgaa gcgcaggtcc tcggtgctct cggcgtacag cacgccgagc
     5281 agccggccgc aggcgcggat cggcacggcg atctggctgc gcgaatcggc gaggcccggc
     5341 agcgggattt cggtttcgag cgcatcgagc aggccgccgt cggcggccgc cgcgcggatc
     5401 gcgcggccgt agccgtattc ggccgtggca tggccgatgc ggaccggcgt gccgacgcgc
     5461 gcggcaacgc cgatcaggcc ctcgccgagc gcgatctcgg cgccgatgcc cgaggcgccg
     5521 tagccgcggc tcgcgagcgt gtacaggcgc tcgccggctt catcgagcat cagcagcagc
     5581 cagtggcgca cgtcgagttc gcgctcgaac acggccagcg cgccggcgag cagctcgtcg
     5641 agttcggtgt acgcattcag gctttccgag caggcgcgca gcaccgccag cggcgaacgc
     5701 cgcggcgcgg gtgcggcgag tgccgggccg ggcacgcgct cgatcgacag cacgcgatag
     5761 acatcggagc cgagcagccg gaacacgccg ctcatgccgg tgtgcgaggc gatgccggcg
     5821 agcttcgcct tcatgcgctc gaacagcgcc ccgccggtct cggtgcgcag gtagtgcagc
     5881 agcaggcggt ggcgcgcgcc ggtgcgcgga tgcgtgacga tcagccgcgc gaccggatgc
     5941 gcgagcacgt tctgccgcgt gcggttgaag aactggtacg acagcgcgac gtgcgcatca
     6001 tcgacgtact cgacctgcga cagatacgcg acgttcggct cgccctcggc cgagcaggtg
     6061 gcgaggatgg ccgggatcgc gccgtccaga cagtcgcgga tggcatcgac gccgctcacg
     6121 gcgcgtgccc ttcgccgccg gcggccacga gcagcgcacc ggctgccggc cccggcgtct
     6181 gcacgaaggc cgcatgcggc gtgaagtcga tcgcggcgag atcctcgtcg gtcgcggcaa
     6241 ccagcatgga catgaccgcg gccgggtaac cgagcgcttc gaggccgccg acgaaggccg
     6301 cccggtagcg cgcaacgcag tcgcgatccc cggcttcgag ggcgccgatg cgggcgtcgc
     6361 gggtcttgag ctgcaccgtg cggtgcgtcg ccggcaggct gcagaccatc gccagcgtcg
     6421 cgttcgccgc cacgtcggcg agcagcgccg ccgaggcgct ggccgcgagc cag
//
LOCUS       DAOQJH010000037         6349 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_6895_length_6349_cov_5.524944, whole genome shotgun sequence.
ACCESSION   DAOQJH010000037 DAOQJH010000000
VERSION     DAOQJH010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 6349)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6349)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6349
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6895_length_6349_cov_5.524944"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1470)
                     /locus_tag="PLQ18_01620"
     CDS             complement(<1..1470)
                     /locus_tag="PLQ18_01620"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNE00377.1"
                     /translation="MPPFRPTAGLTTGVLALALAACTVTPTPVTDGERQARVSADLAQ
                     LTRPVEPVTAPITLDEALRRAVLHNLDQRVKLMETAVAQGQLDVVRWDMLPQLSAQAG
                     YRSRSNDAGSFSKNLSTGVENPDVITSAEDRTRRTAGLVLSWNVLDFGLSYARARQAA
                     DQTLIAAERQRKVLHNIRQDVRQAYWRAWTAQRLLPEVTRLETRTGRALGESREIERQ
                     KLAEPLVALGYQKSLLDTLRELATRRQELELARTELAALMGLPPGTAFRLAPPPANDP
                     VLLALVKTPVEQLIERAFAARPELRQEDYQKRITAMEANKALLGLFPNLNIVGGRSYD
                     SNHYLLNNTWNEVGAGLSLGLLKLFSLPDTLELVERQQQLDELRRLQLGMAIMVQVHL
                     AVQRHALASDDLKLAAAAAGVEQRIVGQTRSAVGAQTAHPLDLARAEADGLLAEVRRA
                     LAEAEVQASLARLAASIGEDPAVPGAGTTTAGTPPGPVTT"
     gene            complement(1823..2662)
                     /locus_tag="PLQ18_01625"
     CDS             complement(1823..2662)
                     /locus_tag="PLQ18_01625"
                     /EC_number="1.13.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol ring-cleavage dioxygenase"
                     /protein_id="HNE00378.1"
                     /translation="MSAETIPAPGSTPGTAPDAAAALPTLFLSHGSPMLSLLEAPARD
                     FLEALGPELPRPRAILVASAHWETAQPSVNAVEHNETIHDFYGFPQALFELRYPAPGS
                     PALAARVADLLHEAGLPCRSDARRGLDHGAWSVLRLMYPDADIPVVQVSLQRGPGPAH
                     HLELGRALAPLRHEGVLVIGSGSFTHDLRRFRGQPIDAPDAPDIAPFCEWMDTALREG
                     RTADLLGYRQRAPYALAQHPSEEHLLPLFVALGAAGPAARARRLHASNTYGMLRMDAY
                     AFA"
     gene            2812..3744
                     /locus_tag="PLQ18_01630"
     CDS             2812..3744
                     /locus_tag="PLQ18_01630"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNE00379.1"
                     /translation="MSPKPTVPDAGPCRLDWDDLRHFLAIARHGTLSGAARALGVRQS
                     TMSRRLAALEARAGAQLLQKTPDGYRPSAAGEAILGPVERIEAEADTVARTITGTDLR
                     LAGSVRITSVETLATLVLMPVLAGFARRYPDIELELLADSRDLDLSRREADVALRLAP
                     YTQPGVVARRLARVGFGVYASADYLDRHGRPDFAAGAPGQYTVLPSDELRQAPEMQWF
                     AARTHAARPGLRCNRRQAALAAIEAGMGIGCLARYLGDGRGLVRLDAGPPPPARALWY
                     GVHEDIRHTARIRALAAALAEGFAAAAMRLDPPD"
     gene            complement(3766..4248)
                     /locus_tag="PLQ18_01635"
     CDS             complement(3766..4248)
                     /locus_tag="PLQ18_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011358732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HNE00380.1"
                     /translation="MSGLHRITPLARTALQHGGRCFAIAALLICAAVPAHAGRVHHTH
                     AGKSASAAGSAQGAGDHDQIGVASYYSSRFSGRRTASGERFDPSAMTAAHMTLPLGTR
                     VRITNLKNGQSVVVRINDRGGFRRHGRILDLSPAAARALSMTGAGVARVGLKRVGGDS
                     "
     gene            4968..5447
                     /locus_tag="PLQ18_01640"
     CDS             4968..5447
                     /locus_tag="PLQ18_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018991431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNE00381.1"
                     /translation="MTDPRPDPTADTLLYTPENYTPTESVGYLLGQAKGRITAAIDAE
                     MAPYDITGAQWITLLHLSCTEVETAGELCKRACVDTGSMTRMLDRLEKKGLVQRVRCC
                     EDRRVVRLRMTEAGEKLAALMPDVASRVLNHHLRGFSRDELELLKSLLRRIIANADG"
     gene            5527..>6349
                     /locus_tag="PLQ18_01645"
     CDS             5527..>6349
                     /locus_tag="PLQ18_01645"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNE00382.1"
                     /translation="MTKTSAVPVSASPSPIRPARRAGLGLLAAALLAACADPSGIAGQ
                     SAYTDASALAAQRTLTEARAATAAWPTEGWWRAWNDPGLDRLIGDALSDNPNLKLAEA
                     RLRNAQALAGLADANRGPRLDAGATLSRERLPEGYIYPAPLGGSTQTDARLGLSFSWE
                     LDLWGRRQAEAAAALDRASAAEVEAQAARLMLAVAIARGWNDLDGRYAQHDVAAAEVE
                     QRRQVLELTRQRVAAGLDSSVELKQAEGAVPAARQQLAATEEGIRLARTQLAALAG"
BASE COUNT          968 a         2184 c         2269 g          928 t
ORIGIN      
        1 cgtcgtgacg gggccgggcg gcgtgcccgc cgtcgtggtg ccggcgcccg gcacggccgg
       61 gtcctcaccg atgctggcgg cgagccgcgc gaggctcgcc tgcacctcgg cctcggcgag
      121 cgcgcggcgc acctcggcga gcaggccgtc ggcctcggcg cgcgccaggt cgagcgggtg
      181 cgcggtctgc gccccgacgg cgctgcgcgt ctggccgacg atgcgctgtt cgacgccggc
      241 ggcggcggcg gcgagcttca ggtcgtcgct ggcgagcgcg tggcgctgca cggccagatg
      301 cacctgcacc atgatcgcca tgccgagctg cagccggcgc agctcgtcga gctgctgctg
      361 gcgctcgacc agctccagcg tgtcgggcag cgagaacagc ttcagcagcc cgagcgacag
      421 gccggcaccg acctcgttcc aggtgttgtt gagcaggtag tggttgctgt cgtagctgcg
      481 cccgccgacg atgttgaggt tcgggaacag cccgagcagg gccttgttcg cctccatcgc
      541 cgtgatgcgc ttctggtagt cctcctggcg cagctccggg cgcgcggcga aggcgcgctc
      601 gatcagctgc tcgaccggcg tcttcaccag cgcgagcagc accggatcgt tcgccggcgg
      661 cggcgcgagc cggaaggcgg tgccgggcgg caggcccatc agcgccgcga gttcggtgcg
      721 cgccagttcg agttcctgac ggcgggtcgc gagttcgcgc agcgtgtcga gcagggattt
      781 ctggtagccg agcgcgacca gcggttcggc gagcttctgg cgctcgatct cgcgcgattc
      841 gccgagcgcg cggccggtgc gcgtttcgag gcgcgtgact tcgggcagca ggcgctgggc
      901 ggtccaggcc cgccagtagg cctggcgcac gtcctgacgg atgttgtgca gcaccttgcg
      961 ctggcgttcg gcagcgatca gcgtctggtc ggcggcctgg cgggcgcgcg cgtacgaaag
     1021 cccgaaatcg agcacgttcc agctcagcac cagtccggcc gtgcgccgcg tgcggtcctc
     1081 ggccgaggtg atcacgtcgg ggttctcgac gccggtcgag aggttcttgc tgaacgagcc
     1141 ggcgtcgttg ctgcgcgagc ggtagccggc ctgcgcgctc agctgcggca gcatgtccca
     1201 gcgcaccacg tcgagctgac cctgcgcgac cgcggtttcc atcagcttca cgcgctggtc
     1261 gaggttgtgc aggaccgccc ggcgcagcgc ctcgtcgagc gtgatcggcg cggtgaccgg
     1321 ctcgaccggc cgggtcagct gcgccagatc ggccgagacg cgcgcctgtc gctcgccatc
     1381 ggtcacgggg gtcggcgtga cggtgcaggc agcgagcgcg agtgccagca cgccggtggt
     1441 caggccggca gtcggtcgaa acggaggcat cggcatggtt tccttacgca gggtgagtcg
     1501 ggtcgcgtca cggcggacgc gtccgggcaa ggggttgcga aggcgcagag atttgccaga
     1561 agccggctgc ggcaactgta aatattcctg acagcttcgc cggcgccagc ctcaccggat
     1621 ccggcaggga tggcgtgtgc cgggatgtcg gcaggaaacg gaaaaacctg aatttccgtt
     1681 catcttcgcg cttttccggc gccggaggcc ggccgaagac aaagcccggg catgcggcac
     1741 cggcgccggc ccgggcttgc agagattgct tcaaggcgac ggcggcggtt gtgcacccgc
     1801 aacaaaccac cgctgccgtg tttcacgcga atgcgtaagc atccatgcgc agcatcccgt
     1861 aggtgttgct ggcgtgcagg cgcctggcgc gcgcagcagg gccggcggcg ccgagggcga
     1921 cgaacagcgg cagcaggtgt tcctcgctcg ggtgctgggc gagcgcgtac ggcgcgcgct
     1981 ggcggtagcc gagcagatcg gccgtgcgcc cctcgcgcag cgccgtgtcc atccactcgc
     2041 agaacggcgc gatgtcgggc gcatccggcg catcgatcgg ctggccgcgg aagcggcgca
     2101 ggtcgtgcgt gaagctgccc gagccgatca ccagcacgcc ttcgtgacgc agcggcgcca
     2161 gcgcgcggcc gagttcgagg tgatgcgccg gcccggggcc gcgctgcagc gagacctgca
     2221 cgaccgggat gtcggcgtcc ggatacatca gccgcagcac cgaccaggca ccgtgatcga
     2281 gcccgcgccg ggcatcgctg cgacacggca ggccggcttc gtgcagcaga tcggcgacgc
     2341 gcgcggcgag cgccggcgag ccgggcgccg gatagcgcag ctcgaacagc gcctgcggga
     2401 agccgtagaa gtcgtgaatc gtctcgttgt gctcgacggc gttgacgctc ggctgcgccg
     2461 tctcccagtg cgcggaggcg accaggatcg cgcgcgggcg cggcagctcc gggccgagcg
     2521 cttcgaggaa atcgcgcgcc ggggcttcga gcagcgacag catcggcgag ccgtggctga
     2581 ggaacagcgt cggcagggcg gcagcggcat cgggggccgt gccaggagtg ctgcccgggg
     2641 cagggatggt ttcggcggac atggcggact ccggatggca tcgggccgca cgcaggcggc
     2701 gatacctgca tcgtgtggtc tgcatcgacg catcaccatt gccgaaaacc gcgcactatg
     2761 tttgcgaaaa cgcatattgc gaaaacgcat gcaccgctgc gggagcgcgc gatgtcaccg
     2821 aagccgactg ttccggatgc cggcccgtgc cggctcgact gggacgacct gcgccatttc
     2881 ctcgcgattg cccggcacgg cacgctgtca ggggcggcgc gcgcgctcgg cgtgcgccag
     2941 tcgacgatgt cccggcgtct ggcggccctc gaagcccgcg ccggcgcaca actgctgcag
     3001 aagacgccgg acggctaccg gccgagcgcc gccggcgagg ccatcctcgg gccggtcgag
     3061 cgcatcgagg ccgaggccga caccgtcgcg cgcacgatca ccggcaccga cctgcgcctg
     3121 gccggcagcg tgcgcatcac cagcgtcgag acgctggcaa cgctggtgct gatgccggtg
     3181 ctcgccggtt tcgcacggcg ctatccggac atcgagctcg aactgctcgc cgacagccgc
     3241 gatctcgacc tgagccggcg cgaggccgac gtggcgctgc gtctggcgcc gtacacgcag
     3301 ccgggcgtgg tcgcgcgccg gctggcgcgc gtcggcttcg gcgtgtacgc gagtgcggac
     3361 tacctcgatc gccacggccg gccggacttc gccgccgggg cgccgggcca gtacacggtg
     3421 ctgccctcgg acgagctgcg ccaggcgccg gaaatgcagt ggttcgcggc gcgcacgcac
     3481 gcggcacgcc cgggcctgcg ctgcaaccgc cggcaggcgg cgctggcggc gatcgaggcc
     3541 ggcatgggca tcggctgtct ggcgcgctat ctcggtgacg gccgcggact cgtgcgtctc
     3601 gacgccggtc cgccaccgcc ggcgcgcgcg ctgtggtacg gcgtgcacga ggacatccgc
     3661 cacaccgcgc gcatccgcgc gctggccgca gccctcgccg aaggcttcgc agccgccgcg
     3721 atgcgcctcg atccgccgga ctgagagccc ggcgcggcct gcggctcagc tgtcgccgcc
     3781 gacccgcttg aggccgacgc gcgcgacgcc ggcgccggtc atcgacagcg cccgcgccgc
     3841 ggccggcgac aggtcgagga tgcggccgtg gcgccggaag ccgccgcggt cattgatgcg
     3901 cacgacgacg ctctggccgt tcttcaggtt ggtgatgcgc accctggtgc cgagcggcag
     3961 cgtcatgtgc gcggcggtca tggccgaggg gtcgaagcgc tcgcccgagg ccgtgcgccg
     4021 gccgctgaag cggctgctgt agtacgaggc cacgccgatc tggtcatggt cccccgcgcc
     4081 ctgcgcgctt ccggcggccg atgcgctctt gccggcgtgg gtgtggtgca cccggccggc
     4141 gtgggcgggg acggcggcgc agatcagcag cgcggcgatg gcgaaacaac ggccgccgtg
     4201 ctgcagggcg gtgcgggcaa gaggagtgat gcgatgtagt ccggacatgg gctgcatccc
     4261 cgagaaggtg gtttggccgt ctcaagtacc cgattccgca gcatggaaaa acacattggt
     4321 caaattttgt ctgaaataca acaatgcttg ctcatcaatg acatagcatg catgtctgaa
     4381 aattaaaaga tacaaatatc gctgtttgca tctgcgtttc agatggcagc cgcgccggca
     4441 cgaaccggca cgcactgcgc gccaccggcc ggccccgaaa ggcaggaatc tgcgcaagtt
     4501 accgtatgga aaggacttcc ggattctgcc tgcagcggcg cagcgcgccg gccacgcaat
     4561 ggccgctttc ccggatcagc ccggtttgag catcggttta cgtgcgttta cggatacgga
     4621 atcatccata cagatatatt tttatctgta ttaaatcggt gacgctgaac gacgatgacg
     4681 cctgaagcgc catccgtgtc aaacacgaca caatcctgaa tcaggaccgg aaccagaccc
     4741 tgaaccggaa acggatgcag gccgcaatgc ctgccggatc ccagggcttc gctgcgggtc
     4801 ggcgcccggt ctgaccagta ttcgtgcaga tatgcattga aagcaggata tcaggcaaac
     4861 atccatacga ttctgcacag tctgacgtgc agtttctgca ttgccattct ctgcataggc
     4921 agttaaattg ccgacccatg tcgataccgt ccacccagaa cgccgccatg accgatccaa
     4981 ggcctgatcc aacagccgac acgctgctgt acacccccga gaactacacg ccgaccgaga
     5041 gcgtcggtta cctgctcggt caggccaagg gccggatcac ggcggcgatc gacgccgaga
     5101 tggcgccgta cgacatcacc ggcgcgcagt ggatcacgct gctgcacctg tcctgcaccg
     5161 aggtcgagac cgccggcgaa ctctgcaagc gcgcctgcgt cgataccggc tcgatgacgc
     5221 gcatgctcga ccggctcgaa aagaagggac tggtgcagcg cgtgcgctgc tgcgaggacc
     5281 gtcgtgtcgt gcgcctgcgc atgaccgaag ccggcgagaa gctcgcggcg ctgatgcccg
     5341 atgtcgcctc gcgcgtgctg aaccatcacc tgcgcggctt ctcgcgcgat gaactcgaac
     5401 tgctgaagtc cctgctgcgc cggatcatcg ccaacgccga cggctgaacc gccggtcccg
     5461 tcaccctgtc gcaccgcgtg cgtcgcaata actgctcagg cagataaaag acaggcagaa
     5521 accgccatga caaagaccag cgccgttccc gtctccgcaa gcccttcccc gatccgccct
     5581 gcccgccgcg ccggcctggg cctgctggct gcggcgctgc tcgccgcctg cgccgatccc
     5641 tccggcatcg ccggccagtc tgcgtatacc gatgcaagcg cgctcgccgc gcagcgcacg
     5701 ctcaccgagg cgcgcgccgc gaccgcggcc tggccgaccg aaggatggtg gcgggcgtgg
     5761 aacgaccccg ggctcgaccg gctgatcggc gatgcgctgt ccgacaatcc gaacctgaag
     5821 ctcgccgagg cgcggctgcg caacgcccag gcgctcgccg ggctcgccga tgccaaccgc
     5881 ggcccgcgcc tcgacgccgg cgccaccctc agccgcgagc gcctgcccga gggctacatc
     5941 tacccggcgc cgctcggcgg cagcacgcag accgacgccc gtctgggcct gagcttctcg
     6001 tgggagctgg atctgtgggg ccgccgtcag gccgaggccg ccgcggcgct cgatcgcgcc
     6061 agcgccgccg aggtcgaggc gcaggccgcg cggctgatgc tggcggtggc gatcgcccgc
     6121 ggctggaatg atctcgatgg ccgctacgcg cagcacgacg tggccgcggc cgaggtcgag
     6181 cagcgccggc aggtgctgga gctgacgcgc cagcgcgtcg ctgccggtct ggattcgtcg
     6241 gtcgagctga agcaggccga aggcgcggtg ccagccgcgc gccagcagct cgcggcgacc
     6301 gaggaaggca tccgtctggc gcgcacgcag ctcgccgcgc tcgccggcc
//
LOCUS       DAOQJH010000038         6332 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_6926_length_6332_cov_4.592003, whole genome shotgun sequence.
ACCESSION   DAOQJH010000038 DAOQJH010000000
VERSION     DAOQJH010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 6332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6332
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6926_length_6332_cov_4.592003"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..470
                     /locus_tag="PLQ18_01650"
     CDS             <1..470
                     /locus_tag="PLQ18_01650"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HNE00383.1"
                     /translation="AAIEARFRSRISTAPVPDGVLALSDDDLRGTGLSRQKAAAIRDI
                     AARAASGFLPDRTEADRLTDDELVSRLTAARGVGRWTVEMFLIFTLGRPDVWPVDDYG
                     VRRGWCVIEANHGLPRARELLHWGEAWRPWRSVLAWYLWRAAEDGSTGTPAAQ"
     gene            complement(658..1044)
                     /locus_tag="PLQ18_01655"
     CDS             complement(658..1044)
                     /locus_tag="PLQ18_01655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE00384.1"
                     /translation="MTPRRARVVESKIIIPICPVNGRFSAVVQTVLLHPTPEGVHGAM
                     PIRADLSHIDLAAMSLPDLHRLVDKIEKAIEIKLFERGAQRALAVFSAHAPTPAAAPP
                     VGRGGNRDVRRTGRERSGRDSGLRRR"
     gene            1288..1707
                     /locus_tag="PLQ18_01660"
     CDS             1288..1707
                     /locus_tag="PLQ18_01660"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNE00385.1"
                     /translation="MSRTTAGTEILQNAVSVPVQRVVSGAAALMMRGMSAALNPEQTA
                     MSMHALHAMPGPQGHGYDSVHVPHAVITQVVVYGDTPLRAWREYLGISRETVAERMGV
                     SARVIRAWEENPLTPPRRSALVPLARALGIDVSRFDL"
     gene            1804..2589
                     /locus_tag="PLQ18_01665"
     CDS             1804..2589
                     /locus_tag="PLQ18_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HNE00386.1"
                     /translation="MSASPLPLIPSIPSSDPERRFGGIARLYGSDALARLQAARVVVV
                     GVGGVGSWAVEALARSAIGTLRIIDLDIVHESNVNRQLPALDGEFGRAKVEVLAERIG
                     RINPACTVEAVEDFLTPENAAGLLAGADAVLDAIDNVRAKAALIACCRSAHLPLVVSG
                     GAGGKQDPGRIRLSDLAQTEQDPMLAKLRQRLRKEYGFPRDPARRFGIDCVWSPEPVL
                     RPRSCAGDAGLNCAGYGASMCMTASFGLRAAARLIERLSASGR"
     gene            complement(2625..3437)
                     /locus_tag="PLQ18_01670"
     CDS             complement(2625..3437)
                     /locus_tag="PLQ18_01670"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNE00387.1"
                     /translation="MKATDLIDDLNRRFADDDYGRRAPAALQRSSDGAVGSFQGLRLS
                     TGVETLFAADGGISGHQAVTVVRGPHGRPLSAWVPYALALDAQAIGWLDRLVRTLHVF
                     NARAVLSLGRLWLAVHPLHLGAISGEFGAVFANLLARMRVHPSQIVLEIADADRMPRS
                     RLMQALSGFRTHGFGTACVLRGDDPGAFEQVLSLHPDAVKLCRSHLYAARASLLGRER
                     LARRIAAIRAAGAHAWLRGVDDLPSTRLARALDADGWQSGLLDARMLGGHGG"
     gene            3704..3937
                     /locus_tag="PLQ18_01675"
     CDS             3704..3937
                     /locus_tag="PLQ18_01675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE00388.1"
                     /translation="MQSCYRRWESRDRWYEASIGTDLFGQLTLTCRWGALRSARGGMQ
                     CRPLTGAAEAGRLLQALARRRERHGYRIVAVLA"
     gene            complement(3949..4467)
                     /locus_tag="PLQ18_01680"
     CDS             complement(3949..4467)
                     /locus_tag="PLQ18_01680"
                     /inference="COORDINATES: protein motif:HMM:NF016874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HNE00389.1"
                     /translation="MKPECPVIYLAGPAVFHPDAAGIGRRLQALCTAAGCKGLFPLDA
                     EVPGDAGRGPAQAAAILQANLALLERADAVLADCTPFRSPSLDPGTAFEIGYAVARGK
                     PVAGWSETAGAYRLRVAQAVRCASGWRDAGGWVIEDFGLPDNLMISASLVAMADDPAG
                     ALAQLLAHLRAR"
     gene            4799..5905
                     /locus_tag="PLQ18_01685"
     CDS             4799..5905
                     /locus_tag="PLQ18_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002555517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium:proton antiporter"
                     /protein_id="HNE00390.1"
                     /translation="MSALLRGEKPLLSAIAVILIAYPLEHTLIDSGRSMALIASGVLI
                     AVILSVALRVAHHAEILADRYGEPYGTMILTISAVLVEVVILAILLSHNPSPTLVRDT
                     IYSALMLDINGILGLAALLGGLRHGVQAYNVDSGNTYLTMILCAVGVSMIVPEFIPHM
                     TWKAYSIFNILVMLLLYALFLRLQTGQYAGFFSYRYAHRAGQSPQELHAESEAAHDPS
                     AVRNSAALLVTGLVLIGALSEVMAKTIDVSLAGSGMPVIVPGMLVALISASPEIMTAL
                     RAALEDRMQPVVNIALGASLSTVILTVPVIEAIALLSGQPIEMALTAPQTVMMVLTLL
                     AATINLHDGETNAIEGTAHFVLFLTFLMLAALGV"
     gene            complement(5921..>6332)
                     /locus_tag="PLQ18_01690"
     CDS             complement(5921..>6332)
                     /locus_tag="PLQ18_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020563567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HNE00391.1"
                     /translation="LELRAWPDAADALRALKAEGLRLAFLSNFTPAMLDRAVTGSGLD
                     GLFDEHLSTDRVRAFKPDPRAYAMAEGFWKLPREAIAFAAFGGWDAAGARAYGYPTFW
                     VNRAGVPAERLDTLADASGATLTELAAWVRMTRG"
BASE COUNT         1029 a         2178 c         2118 g         1007 t
ORIGIN      
        1 cggccgcgat cgaggcgcgt ttccgcagcc ggatcagtac ggcaccggtt ccggacgggg
       61 tgctggccct gagcgatgac gatctgcgcg gcaccggcct gtcgcgccag aaggcggctg
      121 cgatccggga catcgccgca cgtgccgcga gcggctttct gcccgatcgg accgaagcag
      181 acaggctgac ggacgatgaa ctcgtcagcc ggctcacggc cgcgcgcggc gtcgggcgct
      241 ggaccgtcga gatgttcctg atcttcacgc tcggacggcc cgacgtctgg ccggtcgacg
      301 attacggggt gcgccggggc tggtgcgtga tcgaggccaa tcacggattg ccgcgggccc
      361 gggaactgct gcactggggg gaagcctggc ggccgtggcg cagcgtgctc gcctggtatc
      421 tgtggcgggc tgccgaagac ggatcgaccg gcacaccggc agcgcaatga gcgccgggca
      481 ggcagcggtc ggtcgtgacc atggccggat tgtggattgc acgcagccga atgagccggt
      541 gccacggaaa acgccagccc ggtcagcagt gaggaatgct gtcccgcatg atattcggaa
      601 taacagaacc gccggccaat cagcgtcggc tatggccgtc gaatgcgggg gtgtccgtca
      661 gcgccggcgc agtccggaat cccgtccgct gcgctcgcga ccggtcctgc gcacgtcgcg
      721 gttaccgccg cgacccacgg gcggagccgc cgcaggggtc ggggcatgcg cggaaaacac
      781 ggccagagcg cgctgggcgc ctcgctcgaa aagcttgatt tctatggctt tttcaatttt
      841 atccacgagt cggtgcaaat ccggcagtga catcgcggca agatcgatat gggaaaggtc
      901 ggctctgatc ggcatggcgc cgtgtactcc ctccggagtg gggtgcagta ataccgtttg
      961 gacaacggca gaaaaccgcc cgttcaccgg gcagatcgga atgatgatct tcgattcgac
     1021 gacgcgtgcg cgtcgaggcg tcatgacgcc ctgaactggt gcagctgacc ggaatccgga
     1081 cgagtgatgc ctcgaacgat gccgcagcgt caggtgcagg catttcgatc gatcggtcct
     1141 gatcgggtaa tgggccgagt gtatcgcagc tttcagcgaa tgcagtgctt ccggacgagg
     1201 tcgtatgcaa atttccacga cttgccgcct gcgcggcagg cagggattgc cgcagctgaa
     1261 ccgccgcccg gtaacggggt cacacgcatg tcccggacga cggcaggaac ggaaatcctg
     1321 caaaacgctg tgtcagtccc cgtgcagcga gtcgtttccg gcgccgcagc gctcatgatg
     1381 cgcggcatgt ccgcagccct gaatccggag cagaccgcca tgtccatgca cgcactgcac
     1441 gccatgcccg gcccgcaggg gcatggctat gacagcgtcc atgtgcctca tgcggtgatc
     1501 acgcaggtcg tcgtgtacgg agatactccg ctgcgggcct ggcgcgaata tctcggcatc
     1561 agccgcgaga ccgtcgccga gcgcatgggc gtatcggcgc gcgtgatccg ggcctgggag
     1621 gaaaacccgc tgacgccgcc gcggcgctcg gcactcgtac cgctggcccg cgcgctcggc
     1681 atcgatgtga gccgcttcga cctctgagcc ggcacccggc attgcagccg cccacggccg
     1741 ccgctcgacg catctccctg cctcgcctat cgtgccagcc cctgccgccc ggtctgcacg
     1801 ttcgtgtccg cttccccgct gccgctgatc ccgtcgatcc cgtcctctga tcctgaacgc
     1861 cgtttcggcg gcatcgcccg gctgtatggc agcgacgctc tcgcccgcct gcaggcggcg
     1921 cgcgtcgtgg tcgtcggcgt cggcggggtc ggcagctggg ccgtcgaggc actggcgcgc
     1981 agcgcgatcg gcacgctgcg gatcatcgat ctcgacatcg tccatgagtc caacgtcaac
     2041 cggcagctgc cggcactcga cggggaattc ggacgggcga aggtcgaggt gctcgccgaa
     2101 cgcatcggcc ggatcaatcc ggcctgcacg gtggaagccg tcgaggattt cctgaccccc
     2161 gagaacgccg ccggcctgct cgccggtgcc gatgccgtcc tcgatgcgat cgacaacgtc
     2221 cgcgccaagg ccgcgctgat cgcctgctgc cggagcgcgc atctgccgct ggtcgtatcc
     2281 ggcggggccg gcggcaagca ggacccgggg cggatccggc tgtcggacct cgcgcagacc
     2341 gagcaggacc cgatgctggc gaagctgcgc cagcgcctgc gcaaggagta cggctttcca
     2401 cgcgatccgg cgcggcgctt cggcatcgac tgcgtctggt cgccggagcc ggtgctgcga
     2461 ccgcgctcct gtgccggcga tgccggactc aactgcgccg gttatggcgc atcgatgtgc
     2521 atgacggcga gtttcgggct cagggccgcg gcccgcctca tcgagcgcct gagcgcgagc
     2581 ggtcgctgaa agcgcgcggc ccgctcacga agcagggtgt tcgttcagcc gccgtggcca
     2641 ccgagcatgc gggcgtcgag cagaccgctc tgccagccgt cggcatcgag cgcgcgggcc
     2701 agtcgcgtcg agggcagatc gtcgacaccg cgcagccagg catgcgcacc ggccgcccgg
     2761 atcgctgcga tccggcgtgc cagacgctcg cgtccgagca gcgacgcgcg ggccgcatac
     2821 aggtggctgc ggcagagctt gacagcgtcc ggatgcaggg acagcacttg ctcgaacgcg
     2881 cccggatcat cgccgcgcag cacgcaggcc gtgccgaaac cgtgggtccg gaagccggac
     2941 aaggcctgca tcagccgcga gcgcggcatg cgatcggcgt cggcgatctc cagcacgatc
     3001 tgcgagggat gcacacgcat gcgtgccagc agattcgcga acacggcccc gaactcgccg
     3061 ctgatcgccc cgagatgcag cggatgcacg gccagccaca gccgcccgag cgacagcacc
     3121 gcacgggcgt tgaacacatg cagcgtacgc accagccgat ccagccagcc gatcgcctgt
     3181 gcatccagcg ccagcgcata cggcacccac gccgacagcg gccggccatg cgggccgcgc
     3241 acgacggtca cggcctgatg gccgctgatg ccgccatcgg ccgcgaacag ggtttcaacg
     3301 ccggtcgaca ggcgcagtcc ctggaagctg ccaactgcgc cgtcgctgct gcgctgcagg
     3361 gccgccggcg cccgccggcc atagtcatcg tcggcgaacc ggcggttcag gtcatcgatc
     3421 agatcggtgg ctttcatgtc ggaactcccg caaatgacgg ccggccacac cggcctggct
     3481 gctcgaccgg atcgaccggc ctgccgggaa ctttgacagg attcgtgcca tccaaagctt
     3541 ttctgtatgg atcatatact tgccgatatt taatgctgca atgcacgcag gcttctgtcc
     3601 aaaactgtcg ctccatggcg acatgaatgc ctgcattctt ccatgctgat gatttctatg
     3661 gacaatccat gtctttcctc ggcgacagcc cccggagccg gccatgcaat cctgctacag
     3721 acgctgggaa agtcgggatc gctggtatga agccagcatc ggaacggatc tgttcggcca
     3781 actgacgctg acctgccgct ggggggcgct gcgcagtgcc cggggcggca tgcaatgccg
     3841 gccgctcacc ggtgcagccg aagccggacg cctgctgcag gccctggccc ggcggcgcga
     3901 gcgacacggt taccggatcg tcgcagtcct cgcctgagcc cggtggcttc agcgggcacg
     3961 caggtgcgcc agcagttgcg ccagcgcgcc ggccggatca tccgccatcg ccacgaggct
     4021 cgcgctgatc atcaggttgt cgggcagtcc gaaatcctcg atcacccatc ccccggcatc
     4081 ccgccagccc gacgcgcagc ggactgcctg cgccacgcgc agccggtagg ccccggccgt
     4141 ttccgaccat ccggccaccg gcttgccgcg cgcgacggca tagccgatct cgaacgccgt
     4201 gcccgggtcc agcgacgggc tgcgaaacgg tgtgcagtcg gccagcacgg catcggcccg
     4261 ttcgagcagt gcgaggttgg cttgcaggat ggctgccgcc tgcgcgggac cgcgcccggc
     4321 gtcgccgggc acttcggcgt cgagtggaaa cagtcccttg caaccggcgg ccgtgcacag
     4381 tgcctgcaga cgccggccga tgccggcggc gtccggatgg aacacggcgg gtccggcgag
     4441 atagatcacg ggacactctg gcttcatggg gatcggctcc tcaagcgcac acagaccgcc
     4501 cgggtgtagc cggcagcgcg agcgctgtca ggggcccggc gggcagggag gcgggcataa
     4561 aaaaagggcc ggaagaggac cggcccaaaa ggaggaggag aggtaacacc cgacgcagcg
     4621 gatgttgcat tgcaatatgg acgcgatgtt gcagcgcgtc aagggttgtc cgggttgacg
     4681 ggcaaaattt ccggaagcgg ctcccgccag cgggtcggcc ggggccgctc ctatactcgc
     4741 gcgcagttgc ttccctcccc tgcccgaccg aaaggcgctg cgcatgtcgc ccgtatccct
     4801 gtccgctctc ctgcgcggcg aaaagccgct gctcagcgcg atcgccgtca tcctgatcgc
     4861 ctatccgctc gaacacacgc tgatcgacag cggccgcagc atggcgctga tcgcctcggg
     4921 cgtgctgatc gccgtgatcc tcagcgtcgc cctgcgggtc gcacatcacg ccgagatcct
     4981 ggccgatcgc tacggcgaac cctacggcac gatgatcctg accatcagcg ccgtgctggt
     5041 cgaggtcgtg atcctcgcca tcctgctgtc gcacaatccc tcgccgacgc tggtgcgcga
     5101 caccatctat tcggcgctga tgctcgacat caacggcatc ctcggccttg ccgccctgct
     5161 cggcggactg cgccatggcg tgcaggccta caacgtcgat tccggcaaca cctatctgac
     5221 catgatcctg tgcgcggtcg gcgtgtcgat gatcgtgccc gagttcattc cgcacatgac
     5281 ctggaaggct tattcgatct tcaacatcct ggtcatgctg ctgctgtatg cgctgttcct
     5341 gcgcctgcag accggtcagt acgccggttt cttcagctac cgctacgccc accgcgccgg
     5401 tcagtcaccg caggaactgc acgccgaatc cgaagccgca cacgatccgt ccgccgtgcg
     5461 caactcggcg gcgctgctgg tcaccggcct ggtgctgatc ggggcgctgt cggaagtgat
     5521 ggccaagacc atcgacgtga gcctcgccgg cagcggcatg ccggtgatcg tgcccggcat
     5581 gctggtcgcg ctgatctcgg cgagcccgga gatcatgact gcattgcgcg ccgctctcga
     5641 agaccgcatg cagccggtcg tcaacatcgc gctcggtgcc tcgctgtcga cggtgatcct
     5701 gaccgtgccg gtgatcgagg ccatcgcgct gctgagcggc cagccgatcg agatggcact
     5761 gaccgccccg cagacggtga tgatggtgct gacgctgctc gccgcgacca tcaacctgca
     5821 cgacggcgag accaacgcca tcgagggcac ggcgcacttc gtgctgttcc tgaccttcct
     5881 gatgctggcg gcgctcgggg tctgatgccg gctgccggta tcagccgcgc gtcatccgca
     5941 cccaggccgc cagctcggtc agcgttgcac cgctggcatc ggcgagcgtg tcgagccgtt
     6001 cggccggtac gccggcgcgg ttgacccaga aggtcggata gccgtacgca cgcgccccgg
     6061 ctgcgtccca gcctccgaag gccgcgaatg cgatggcctc gcgcggcagc ttccagaaac
     6121 cctcagccat cgcgtacgcg cgcggatcgg gcttgaaggc gcgcacgcga tcggtgctca
     6181 ggtgctcgtc gaacaggccg tcgaggccgc tgccggtgac ggcccgatcg agcatcgccg
     6241 gcgtgaagtt cgacaggaac gcgagccgca ggccctctgc cttcagcgcc cgcagcgcgt
     6301 cagccgcatc cggccaggcg cgcagttcca gc
//
LOCUS       DAOQJH010000039         6321 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_6941_length_6321_cov_4.154485, whole genome shotgun sequence.
ACCESSION   DAOQJH010000039 DAOQJH010000000
VERSION     DAOQJH010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 6321)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6321)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6321
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6941_length_6321_cov_4.154485"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..655)
                     /locus_tag="PLQ18_01695"
     CDS             complement(<1..655)
                     /locus_tag="PLQ18_01695"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HNE00392.1"
                     /translation="MPGKVFVSYSHKDEKWKDRVVEQLGVLGFDGLLEVWEDRQIAAG
                     DDWKPAIQKAIAECDAALLLISASFLTSKFIRGEEVPPLLKRRQEEGIRVVPVILKPC
                     PWTELDWLNPIQARPTDGKPLSKMRPANADEALSRLVSELSGLLKAGKPAGPFDGGGS
                     ATRALPPDPIDLTHLPDATPDFLGRIAELEALDAAWTDDAGTDALVLVAPGGTGKSSL
                     "
     gene            complement(739..1830)
                     /gene="ychF"
                     /locus_tag="PLQ18_01700"
     CDS             complement(739..1830)
                     /gene="ychF"
                     /locus_tag="PLQ18_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005693776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HNE00393.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIQAENYPFCTIDPNVGVV
                     PMPDARLDALAKIVVPQRILPTTMEFVDIAGLVAGASKGEGLGNQFLANIRETDAIAH
                     VVRCFENDDVIHVAGKVDPLSDIEVINTELALADLASVEKAFQRAEKAAKAGGKDAVA
                     RKELLARVQAHLNEAKPVRAMNLSDDELALLYELHLLTVKPTMYIANVHEDGFDHNPL
                     LDKVRALAAAEGAEVVPVCAAIEAEIAQLDDADKLEFLADLGLDEPGLNRVIRAGYKL
                     LGLQTYFTAGVKEVRAWTVPIGATAPQAAGVIHTDFERGFIRAEVIAYDDFIAGNGED
                     GAKKAGKWRLEGKDYVVQDGDVMHFRFNV"
     gene            complement(1871..2458)
                     /gene="pth"
                     /locus_tag="PLQ18_01705"
     CDS             complement(1871..2458)
                     /gene="pth"
                     /locus_tag="PLQ18_01705"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018232590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HNE00394.1"
                     /translation="MAEALKLIAGLGNPGPAYERTRHNAGFWFVDELARSHGGSFRHD
                     PKLNADTCQITVEGRALWLMKPMGFMNRSGQPLARLANFYKIARPSILVVHDELDLPP
                     GVARLKRGGGHGGHNGLRDIISQCGGNDFLRLRLGIGHPGTAAEVVDYVLRKAPSSEQ
                     QLIERALDDALRVLPLVVRDEFERAMQTLHTRAPA"
     gene            complement(2476..3093)
                     /locus_tag="PLQ18_01710"
     CDS             complement(2476..3093)
                     /locus_tag="PLQ18_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020210863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HNE00395.1"
                     /translation="MSVVAFELNAETRSEQGKGASRRLRIAGRVPAVLYGAGKAPELL
                     SVSQRDILLQLENEAFYSHVLDLKIGDRAERVVLRDLQRHPYKPMVLHLDLQRVDATQ
                     QITVHVPLHFVNQEAAIGVKQQGGVVSHHLSEIEVTCLPDQIPEFIEVDLIALELGQA
                     LHVSQLVLPAGCTAYALTHGQHSDEIVVSIHAARAGGDEEAAAAV"
     gene            complement(3197..4135)
                     /locus_tag="PLQ18_01715"
     CDS             complement(3197..4135)
                     /locus_tag="PLQ18_01715"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017925991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HNE00396.1"
                     /translation="MMVFTGRAHPELAQRIVDHLGLPMGKAVTGSFSDGECMVELMEN
                     VRGRDVFIVQPTSAPTNDNLMELLVMVDALRRSSAIRITAVIPYFGYSRQDRRPRSAR
                     VPISAKVVANMITTVGVDRVLTIDLHADQIQGFFDLPVDNIYATPIMLEDLFQLHLPN
                     LVVVSPDVGGVVRARALAKRLHDADLAIIDKRRPKANVSQVMHIIGEVEGRDCILIDD
                     MVDTAGTLCHAAKALKDDGAASVRAYCTHAVLSGKAIDNLSNSALDELVVTDTIPLRP
                     EAKACGRIRQLSVAGLLAETIHRIHLEESVSSLFGE"
     gene            complement(4184..4258)
                     /locus_tag="PLQ18_01720"
     tRNA            complement(4184..4258)
                     /locus_tag="PLQ18_01720"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4224..4226),aa:Gln,seq:ttg)
     gene            complement(4316..5215)
                     /gene="ispE"
                     /locus_tag="PLQ18_01725"
     CDS             complement(4316..5215)
                     /gene="ispE"
                     /locus_tag="PLQ18_01725"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005224661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HNE00397.1"
                     /translation="MSDAGSSPSSPSSSPAFSVGWPAPAKLNRFLHIVGRRADGYHLL
                     QTVFQFIDRADTLAFALRDDGRIERETDLPGVPAEADLVVRAARRLQAETGTRRGVSI
                     RIDKQLPMGGGLGGGSSDAATVLVALNHLWGCGLDDDALAALGLTLGADVPVFVRGRA
                     AWAEGIGERLTPVEPDEPWFLVLVPAVHVATAAIFKAPELTRDSPLITMRAFLGGAGH
                     NDCEAVVHARHPEVAAATAWLSQHTTARLTGTGACVFGAFADEAAARTVMAGRPAGLE
                     AFVARGRNRSPLLDRLARARAGD"
     gene            complement(5227..5841)
                     /gene="lolB"
                     /locus_tag="PLQ18_01730"
     CDS             complement(5227..5841)
                     /gene="lolB"
                     /locus_tag="PLQ18_01730"
                     /inference="COORDINATES: protein motif:HMM:TIGR00548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HNE00398.1"
                     /translation="MVRRSRLWPAAPAALLALALAGCAELPASRGDATQDPRWQARSA
                     QLAKLRDWQLDARIGVNRDRQAWSASLKWQQHGPRYEIDLLGPFGAGRVRVEGDAAGV
                     VLLRGDDEPPVSARDPDELFERATGIAVPAGALGDWLFGRPQPGVAAQVEADAEGRTT
                     RIVQAGWTIEYPAWQPVGALELPAKLRAVRGDVQVRLAVERWGL"
     gene            complement(5835..>6321)
                     /locus_tag="PLQ18_01735"
     CDS             complement(5835..>6321)
                     /locus_tag="PLQ18_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004336283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNE00399.1"
                     /translation="AVLTAALAGHPDDTDLLYARALVAERLDRIGETESDLRRVLELD
                     PEHGQALNALGYTLADRTDRLAEAEDLIRRALEKLPSDAAVLDSMGWVLYRQGKAAEG
                     LPFLERAQGLSLEPEILAHLVEVLWTLGRQDEARALWERGAQRAPGDRFLQQVREKLK
                     W"
BASE COUNT          976 a         2082 c         2264 g          999 t
ORIGIN      
        1 ccagcgagga cttgccggtg ccgccggggg cgacgagcac gagggcgtcg gtgccggcgt
       61 cgtcggtcca ggcggcgtcg agggcttcga gttcggcgat gcggccgagg aagtcggggg
      121 tggcgtcggg gaggtgggtg aggtcgatgg ggtctggcgg caacgcacgg gtggcgctgc
      181 cgccaccgtc aaaagggccg gcaggtttcc cggccttgag caatcccgac aactcggaca
      241 ccagtctgga caaagcctcg tccgcgttgg ctggacgcat cttgctcagt ggcttgccat
      301 cggttggccg tgcctgaatc ggattcagcc agtcaagctc cgtccagggg catggcttga
      361 ggatcacggg gacaacccgg atgccttctt cctgccggcg cttcagcagc ggtggtactt
      421 cttcgccccg gatgaatttc gaagtcagga acgaagcgga aatcagcagc agtgccgcat
      481 cgcattcggc aatggctttc tggatggccg gtttccagtc atcgcctgca gcgatctggc
      541 ggtcctccca gacttccagc agtccatcga aaccgagtac gccgagctgc tcgacgacgc
      601 gatccttcca cttctcgtct ttatggctgt aactgacaaa caccttgccc ggcatcgcgc
      661 accctcccct tccctcgtgc cgcgagcgta gcggccggcg caggggcggg acaatgcggg
      721 cagccggggc agccgcgatc agacgttgaa gcggaagtgc atcacgtcgc cgtcctgcac
      781 gacgtagtcc ttgccttcga ggcgccactt gccggccttc ttggcgccat cctcgccgtt
      841 gccggcgatg aagtcgtcgt aggcgatgac ttcggcgcgg atgaagccgc gttcgaagtc
      901 ggtgtggatg acgccggcgg cctgcggcgc ggtggcgccg atcggcaccg tccaggcgcg
      961 cacttccttg acgccggcgg tgaagtaggt ctgcagcccg agcagcttgt aaccggcgcg
     1021 gatgacgcgg ttcaggccgg gctcgtcgag gccgaggtcg gcgaggaatt cgagcttgtc
     1081 ggcatcgtcg agctgcgcga tctcggcctc gatcgccgcg cacaccggca cgacttcggc
     1141 gccttcggcg gcagcgagcg cgcgcacctt gtcgagcagc gggttgtggt cgaagccgtc
     1201 ctcgtggacg ttggcgatat acatcgtcgg cttgacggtg agcaggtgca gctcatagag
     1261 cagcgccagt tcatcgtcgg aaagattcat cgcccgcacg ggtttggctt cgttcaggtg
     1321 cgcctgcacg cgggcgagca gctccttgcg ggcgacggcg tccttgccgc cggccttggc
     1381 ggccttctcg gcgcgctgga aggctttctc gacgctggcg aggtcggcaa gcgcgagttc
     1441 ggtgttgatg acctcgatgt cggacagcgg atcgaccttg ccggcgacgt ggatcacgtc
     1501 atcgttctcg aagcagcgca cgacgtgggc gatggcgtcg gtctcgcgga tgttggcgag
     1561 gaactggttg ccgaggccct cgcccttcga ggcaccggcc accagcccgg cgatgtccac
     1621 gaactccatc gtcgtcggca ggatgcgctg cggaacgacg atcttcgcga gcgcatcgag
     1681 gcgcgcatcc ggcatcggca ccacgccgac gttcggatcg atggtgcaga acgggtagtt
     1741 ctcggcctgg atgccggctt tcgtcagcgc gttgaacagg gtggatttgc cgacgttggg
     1801 caggccgacg atgccgcact tgaagcccat ggggagggtg tcctcgggat acggggatgg
     1861 ggcggaaggc tcaggccggc gcgcgcgtgt gcagcgtctg catggcccgt tcgaattcgt
     1921 cgcgcacgac cagcggcagc acgcgcaggg cgtcgtcgag ggcgcgctcg atcagctgct
     1981 gttcggacga cggcgccttg cgcagcacgt agtcgacgac ttcggccgcc gtgcccggat
     2041 ggccgatgcc gaggcgcagg cgcaggaaat cgttgccgcc gcactgactg atgatgtcgc
     2101 gcaggccgtt gtggccgccg tggccgccgc cgcgcttgag gcgcgcgacg ccgggcggca
     2161 ggtcgagctc gtcgtgcacg acgaggatcg agggccgcgc gatcttgtag aaattggcca
     2221 gccgggcgag cggctggccg gagcggttca tgaagcccat cggcttcatc agccagagcg
     2281 cacggccttc gaccgtgatc tggcaggtgt cggcgttgag cttcgggtcg tggcggaagc
     2341 tgccgccgtg gctgcgggcg agttcgtcga cgaaccagaa gcccgcgttg tgccgcgtgc
     2401 gctcgtaggc cggcccgggg ttgccgaggc cggcgatgag tttcagcgct tccgccatga
     2461 ccgcacgcgc gccgcttata cggcggccgc ggcttcctcg tcgccaccgg cacgggcggc
     2521 gtggatgctg acgacgatct cgtcgctgtg ctggccgtgg gtcagcgcgt aggccgtgca
     2581 gccggccggc agcacgagct gcgacacgtg cagcgcctga ccgagttcga gcgcgatcag
     2641 gtcgacctcg atgaactccg gaatctggtc aggcaggcag gtgacctcga tttcgctcag
     2701 gtggtggctg acgacaccgc cctgctgctt gacgccgatc gcggcttcct gattcacgaa
     2761 gtgcagcggc acgtgcacgg tgatctgctg cgtcgcatcg acgcgctgca ggtcgaggtg
     2821 caggaccatc ggcttgtacg ggtggcgctg caggtcacgc agcacgacgc gctcggcacg
     2881 atcgccgatc ttcagatcga gcacgtgcga gtagaaggct tcgttttcga gctgcagcag
     2941 gatgtcacgc tgcgagacgg acagcagttc cggggccttg ccggcaccgt agagcacggc
     3001 gggcacccgg cccgcgatgc gcaggcggcg gctcgcaccc ttgccctgct cactgcgggt
     3061 ttcggcattc agttcaaaag caacgacgct catggcgtac tccggttgac ggtgatgaaa
     3121 cgaccggggc gtccgcgacc agacgcccgg ggcgaaaaaa ccttgaaatc tcgggcagga
     3181 agtccgtgcg cgcggatcac tcgccgaaca gcgagctgac ggattcctcc agatggatgc
     3241 ggtggatggt ctcggcgagc aggccggcga cgctcagctg ccggatgcgg ccgcaggcct
     3301 tcgcttcggg gcgcagcgga atggtgtcgg tgacgacgag ctcatcgagc gccgagttcg
     3361 acaggttgtc gatcgccttg cccgagagca cggcgtgcgt gcaataggcg cgcaccgagg
     3421 cggcaccgtc gtccttcagc gccttggcgg cgtggcagag cgtgccggcg gtatcgacca
     3481 tgtcgtcgat caggatgcag tcgcggccct cgacctcgcc gatgatgtgc atgacctgcg
     3541 agacgttggc cttcggccgg cgcttgtcga tgatcgcgag atcggcatcg tgcaggcgct
     3601 tggcgagcgc gcgggcgcgc acgacgccgc cgacatcggg cgagacgacg accaggttcg
     3661 gcaggtgcag ctggaagagg tcctccagca tgatcggcgt cgcgtagatg ttgtcgaccg
     3721 gcaggtcgaa gaagccctgg atctggtcgg cgtgcaggtc gatggtcagc acgcggtcga
     3781 cgccgaccgt cgtgatcatg ttggcaacga ccttcgccga gatcggcacg cgcgccgagc
     3841 gcggccggcg atcctgacgc gagtagccga agtacgggat cacggccgtg atgcggatcg
     3901 ccgaggagcg gcgcagcgca tcgaccatga ccagcagttc catcaggttg tcattggtcg
     3961 gggccgaggt cggctgcacg atgaacacgt cgcgaccgcg gacgttttcc atcagctcga
     4021 ccatgcactc gccgtcactg aagctgccgg tcaccgcctt gcccatcggc aggccgaggt
     4081 gatcgacgat gcgctgggca agctccgggt gggcgcggcc ggtaaagacc atcatacggg
     4141 cgtcggacac gggtcaacct gccgtcggaa caggggggga aactggctgg gccgcctgga
     4201 ttcgaaccag ggaatgccgg aatcaaaatc cggtgcctta ccgcttggcg acggcccaat
     4261 gatcttgtgc tgcatctggt gctgcatctt gtgtatcggc tgctgcatcg gcttgtcagt
     4321 caccggcccg cgcacgggcc agccggtcga gcagcggcga acggttgcgt ccgcgcgcga
     4381 caaaggcttc gagccctgcc ggtcgcccgg ccatgaccgt gcgcgccgca gcttcgtccg
     4441 cgaacgcacc gaacacgcag gcaccggtgc cggtcagccg cgccgtggtg tgctgcgaaa
     4501 gccatgccgt cgctgccgcg acctcgggat gacgggcatg gacgaccgcc tcgcagtcgt
     4561 tgtgccccgc ccccccgaga aaggcgcgca ttgtgatgag gggggaatcc cgtgtcaatt
     4621 caggcgcctt gaagattgcg gcagtcgcaa catgcaccgc cggcaccagc accagaaacc
     4681 agggctcgtc cggctcgacc ggcgtgagcc gttcgccgat gccttcagcc caggccgccc
     4741 ggccgcgcac gaacaccggc acgtcggcgc cgagcgtgag cccgagagcg gccagcgcat
     4801 cgtcatcgag cccgcagccc cacagatgat tgagcgccac cagcacggtc gcggcatcgg
     4861 acgaaccgcc gccgagaccg ccgcccatcg gcaactgctt gtcgatgcgg atgctgacgc
     4921 cgcggcgcgt gccggtctcg gcctgcagcc gccgcgcggc gcgcacgacg agatccgcct
     4981 cggccggcac gccgggcaga tcggtttcgc gctcgatgcg gccgtcgtcg cgcagcgcga
     5041 aggccagcgt gtcggcccgg tcgatgaact ggaacacggt ctgcagcagg tgatagccat
     5101 cggcccgccg gccgacgatg tgcaggaagc ggttgagctt ggccggggcc ggccagccga
     5161 cggagaacgc cggggaagaa gaaggagagg aaggagaaga accggcgtcg gacatcgcgg
     5221 tacggctcac agcccccagc gctcgaccgc gagccgcacc tgcacgtcgc cgcgcaccgc
     5281 gcgcagcttc gccggcagct ccagcgcgcc gaccggctgc caggccggat actcgatggt
     5341 ccagccggcc tgcacgatgc gcgtcgtgcg gccctcggcg tcggcttcga cctgtgcggc
     5401 cacgccgggc tgcggccggc cgaacagcca gtcaccgagc gccccggccg gcacggcgat
     5461 gccggtggcg cgttcgaaca gctcatcggg atcacgcgcg ctgaccggcg gctcgtcgtc
     5521 cccgcgcagc agcacgacgc cggccgcatc gccctcgacg cgcacgcgcc cggcgccgaa
     5581 ggggccgagc aggtcgatct cgtagcgcgg gccgtgctgc tgccacttca ggctcgccga
     5641 ccaggcctgc cggtcgcggt tgacgccgat gcgcgcatcg agctgccagt cacgcagctt
     5701 tgcgagctgc gcgctgcgcg cctgccagcg cgggtcctgc gtggcatcgc cgcggctcgc
     5761 cggcagttcg gcgcagccgg ccagcgcgag tgcgagcagg gcggcgggcg cggcgggcca
     5821 caggcgcgag cgcctcacca tttcagcttc tcgcgcacct gctgcaggaa gcggtcaccg
     5881 ggcgcgcgct gcgcgccgcg ttcccacagc gcgcgcgcct cgtcctgccg gcccagcgtc
     5941 cagagcacct cgaccagatg cgcgagaatc tccggctcca gcgacaggcc ctgcgcacgt
     6001 tcgaggaagg gcaggccctc ggcggccttg ccctggcggt acagcaccca gcccatgctg
     6061 tcgagcacgg cggcatcgct cgggagcttt tcgagcgcgc ggcggatgag atcctcggcc
     6121 tcggcgagac ggtcggtgcg atcggccagc gtgtagccga gcgcgttcag cgcctgcccg
     6181 tgctcggggt cgagttcgag cacgcggcgc aggtcggatt cggtctcgcc gatgcgatcg
     6241 agccgctcgg cgaccagcgc ccgggcgtag agcaggtcgg tgtcgtccgg atgaccggcg
     6301 agcgcggcgg tcagcaccgc c
//
LOCUS       DAOQJH010000040         6305 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_6959_length_6305_cov_4.782720, whole genome shotgun sequence.
ACCESSION   DAOQJH010000040 DAOQJH010000000
VERSION     DAOQJH010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 6305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6305
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6959_length_6305_cov_4.782720"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(56..574)
                     /gene="purE"
                     /locus_tag="PLQ18_01740"
     CDS             complement(56..574)
                     /gene="purE"
                     /locus_tag="PLQ18_01740"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010584299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HNE00400.1"
                     /translation="MNAPSPLVGLIMGSRSDWSTLEHAAATLETLGVPHEVRVVSAHR
                     TPDLLFEYAGSAEARGLEVILAGAGGAAHLPGMVASKTVLPVLGIPVQSAALNGLDSL
                     LSIVQMPGGIPVGTLAIGKAGAINAALLAVAILANSRPELRKKLESFRDEQTRKVVAE
                     SALDAAPGVNSK"
     gene            complement(789..2309)
                     /locus_tag="PLQ18_01745"
     CDS             complement(789..2309)
                     /locus_tag="PLQ18_01745"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017772343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNE00401.1"
                     /translation="MIYAKPGQPGSKVTFKSRYANFIGGEWVPPVEGRYFENVSPVDG
                     KVFCEIPRSSPADIELALDAAHRAKDAWGKASPTERSNTLLKIADRMEANQEMLAVAE
                     TWDNGKPIRESLAADTALAIDHFRYFAGCIRAQEGSIGEIDHDTVAYHFHEPLGVVGQ
                     IIPWNFPLLMAAWKIAPALGAGNCIVLKPAEQTPVSILVLAELIQDILPPGTLNIVNG
                     FGREVGQALATSKRIAKIAFTGSTATGRLVMQYATENIIPVTLELGGKSPNIFFDDVM
                     AKDDSFREKALEGFAMFALNQGEVCTCPSRALVQESIADEFLALAVERVSRIKQGDPL
                     DTDTMVGAQASADQVEKIMSYIDIGRQEGAQCLIGGERNQLGDSLGRGYYIKPTVFKG
                     NNKMRIFQEEIFGPVLSVTTFKDEAEALEIANDTIFGLGSGVWTRDGVRAYRMGRGIQ
                     AGRVWTNCYHHYPAHAAFGGYKQSGIGRETHKMMLDHYQQTKNLLVCYSQNPRGFF"
     gene            complement(2629..3453)
                     /locus_tag="PLQ18_01750"
     CDS             complement(2629..3453)
                     /locus_tag="PLQ18_01750"
                     /inference="COORDINATES: protein motif:HMM:TIGR00637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HNE00402.1"
                     /translation="MSPIDPPSPADIATQLAFFARDGQTLLKPERVALLEHIQHSGSI
                     SAAARAIPMSYKAAWDAIDAMNNLAPQTLVERVAGGQRGGGSRLTDYGARIVARYRAI
                     ERMQRQLIDLIAMDDPQMLGDLPGLGLLFGLRTTARNLFSGRVARVAAGAVSSEVVID
                     IGAQDRLVAIVGRQDVEALRLVPGAAVLALVQATQVMLALAEAGLRTSARNRLDGTVL
                     CIGPGAVNAEGQIALAGGKRLTAMMTMESLQALGLEPGQPCTAWIKASSIVLAAIG"
     gene            3566..4027
                     /locus_tag="PLQ18_01755"
     CDS             3566..4027
                     /locus_tag="PLQ18_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013261056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HNE00403.1"
                     /translation="MSSASSTLPRPPLKVSAGNQIHGHVVTVERGAVNAEVLIDIGGG
                     ASMVAIVTDESIDALTLRPGREVYALVQASSVIFTTERMPFVSARNALWGVVDALRTG
                     AVNDEISMRLDAGQRVTATLTHTSVHELGLAPGRRACALIKASSVLLAVPA"
     gene            4106..4942
                     /locus_tag="PLQ18_01760"
     CDS             4106..4942
                     /locus_tag="PLQ18_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008972962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HNE00404.1"
                     /translation="MSFRPSLPGLRAGLCALLLSVLSLPVQAAERFITVASTTSTEQS
                     GLFGYLLPKFTGKTGIEVRVVAVGTGQAIKIAQQGDADVLFVHHQPSEEKFVAEGYGV
                     QRFPVMYNDFIVVGPGTDPAAIEGGHDVVAAFRAIAGKQAPFASRGDDSGTHKAEQAL
                     WKAAGIDVKAASGSWYRETGSGMGPTLNTAAGLPAYALADRGTWLSFRNRGPLKIVVE
                     GDKRLFNQYGILLVSPAKFPHVRQADAQAFIDWVISPEGQQAIAGYRINGEQLFFPDY
                     GR"
     gene            5043..5837
                     /locus_tag="PLQ18_01765"
     CDS             5043..5837
                     /locus_tag="PLQ18_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019989400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HNE00405.1"
                     /translation="MRLDAGCELEFEILEPTPMLLMLRPRSGEGQWVEAERYALQPRV
                     PASEYADAWGNLCQRIVARPGRLHISTQVRVDTDDTIALAPGAPRTPVDQLPVEVIGF
                     LLASRYAESDALADQARDVTAGATPGYDQVEAIRAWIHRSFEYRYGVSNESTSARDTI
                     RMRKGVCRDFAHVGIALTRALGIPARYVAGYLHELEPMDLHAWFEAYVDGRWYTFDAT
                     QAGPRGGRVIIGYGRDALDVALATPFGDTKLDNMQAWVRAVPEAAG"
     gene            complement(5984..>6305)
                     /locus_tag="PLQ18_01770"
     CDS             complement(5984..>6305)
                     /locus_tag="PLQ18_01770"
                     /inference="COORDINATES: protein motif:HMM:NF024189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE00406.1"
                     /translation="VTVSRHSVGPATTPPPAPTVTEMTPPPPPGAVVAADVPPPPPRV
                     EQPVGQPAPEYRWVPGHWSWENGAWAWTPGTWEQPPQQSAEWVPGRWEQRPDGWLWIP
                     GYWS"
BASE COUNT          968 a         2123 c         2223 g          991 t
ORIGIN      
        1 agcacgccga gtgtggcgcc ggggagaatc ggcgaaccga tcgaatggct gcgggtcact
       61 tcgagttcac tcccggcgcg gcgtcgagcg cgctctcggc gacgactttc cgcgtctgct
      121 cgtcgcggaa cgactcaagc ttcttgcgca gttcgggccg actgttggcg aggatcgcca
      181 cggccaagag cgcggcattg attgcgccgg ccttgccgat cgccagcgtg ccgaccggga
      241 tgccgcccgg catctgcacg atcgacagca gcgaatcgag gccgttgagc gccgccgact
      301 gcaccgggat gccgagcacc ggcagcacgg tcttgctcgc gaccatgccc ggcaggtgcg
      361 ccgcgccgcc ggcgccggcg aggatgactt cgagtccgcg tgcctcggcg ctgccggcgt
      421 attcgaacag caggtcgggc gtgcggtgcg cggagacgac gcgcacctca tgcgggacgc
      481 cgagggtttc gagcgtcgcg gcggcgtgtt cgagcgtgct ccagtcggac ctggagccca
      541 tgatgagacc gacgagggga gagggtgcgt tcatcggcgc attctaccgg ccacccccgg
      601 tccggccaac gcgcgatgcg aacgcggagc attccgcagc ggccggcgcg caggccgtca
      661 tccggaaagc ggcggcggcg ccgatgaaag cagcgccgat gaaaaaggac ggacgggagc
      721 gtgtccccgt ccgtccccgg catgagcagg cgtcgcgccg gcaggcagcg acgctgcgaa
      781 gcgtcggctc agaagaagcc gcgcgggttc tggctgtagc agaccagcag gttcttggtc
      841 tgctggtagt ggtcgagcat catcttgtgc gtctcgcgac cgatgccgga ctgcttgtag
      901 ccaccgaacg cggcatgcgc cgggtagtgg tggtagcagt tggtccagac gcggccggcc
      961 tggatgccgc ggcccatgcg gtaggcgcgc acgccgtcgc gggtccagac gccggagccg
     1021 aggccgaaga tggtgtcgtt ggcgatttcc agcgcttcgg cttcgtcctt gaaggtcgtg
     1081 accgacagca cggggccgaa gatctcctcc tggaagatgc gcatcttgtt gttgcccttg
     1141 aacaccgtcg gcttgatgta gtacccgcgg ccgaggctgt cgccgagctg gttgcgctcg
     1201 ccgccgatca ggcactgcgc gccttcctga cggccgatgt cgatgtacga catgatcttc
     1261 tcgacctgat cggccgaggc ctgcgcgccg accatggtgt cggtgtcgag cggatcaccc
     1321 tgcttgatgc ggctgacgcg ctcgacggcg agcgcgagga actcgtcggc gatcgattcc
     1381 tgcaccagcg cgcgcgacgg gcaggtgcag acctcgccct ggttcagcgc gaacatcgcg
     1441 aagccttcga gcgccttctc gcggaaggag tcgtccttcg ccatcacgtc gtcgaagaag
     1501 atgttcggcg acttgccgcc gagttcgagc gtcaccggga tgatgttctc ggtcgcgtac
     1561 tgcatgacca gacggccggt ggcggtcgag ccggtgaagg cgatcttggc gatgcgcttg
     1621 ctggtggcga gcgcctggcc gacttcgcgg ccgaagccgt tgacgatgtt cagcgtgccg
     1681 gggggcagga tgtcctggat cagctcggcg agcacgagga tcgagaccgg ggtctgctcg
     1741 gcaggcttca gcacgatgca gttgccggca ccgagcgccg gggcgatctt ccacgccgcc
     1801 atcagcagcg ggaagttcca cgggatgatc tggccgacga cgccgagcgg ctcgtggaag
     1861 tgataggcaa ccgtgtcgtg gtcgatctcg ccgatcgaac cttcctgcgc gcggatgcag
     1921 ccggcgaagt agcggaagtg atcgatcgcg agcgcggtgt cggcggcgag cgattcacgg
     1981 atcggcttgc cgttgtccca ggtctcggcg accgcgagca tctcctggtt cgcttccatg
     2041 cggtcggcga tcttcagcag ggtgttcgaa cgctcggtcg gcgaggcctt gccccacgca
     2101 tccttggcgc ggtgtgcggc atcgagcgcc agctcgatgt cggccggcga ggagcgcgga
     2161 atctcgcaga acaccttgcc gtcgaccggc gagacgttct cgaagtagcg gccttcgacc
     2221 ggcggcaccc actcgccgcc gatgaagttg gcgtagcgcg acttgaaggt gaccttcgaa
     2281 ccgggctggc caggctttgc ataaatcatg aggctctcct gcatcttctt tgatgggtgg
     2341 tcgtcacggc cgtctgctgc ggtcgtgcca gtttgggcag gagagccatc gcaatatgga
     2401 tgccattcta taaagtgaca ttcataaaaa aacttcctgt gggaaaacag gagtttgtga
     2461 cattgcggaa aattcattcc catgaaaaag ccaatggaaa tcgtccctgc agcggcacag
     2521 gctgtgacgc cgctccggcg cagacaaggc gctgcgccgg gtgcgacaca tacaactgat
     2581 tgatacgaaa gaggtaggcg aaaccgcggc ggggcgggtg ccggggcttc agccgatggc
     2641 ggccagcacg atgctcgaag ccttgatcca ggccgtgcag ggctgacccg gttcgaggcc
     2701 gagcgcctgc aggctttcca tcgtcatcat cgccgtcagc cgcttgcccc cggcgagcgc
     2761 gatctgcccc tcggcattga cggcgcccgg gccgatgcag agcacggtgc cgtcgagacg
     2821 gttgcgggcg ctggtgcgca gcccggcctc cgccagcgcc agcatcacct gcgtggcctg
     2881 caccagcgcc agcaccgccg cccccggcac caggcgcagg gcctcgacat cctggcgccc
     2941 gacgatggcc accagccggt cctgggcgcc gatgtcgatg accacctcgc tcgacaccgc
     3001 accggccgcg acccgcgcca cccgcccgga aaacaggttg cgcgccgtcg tgcgcagccc
     3061 gaacagcagg ccgagaccgg gcaggtcgcc gagcatctgc ggatcgtcca tggcaatcag
     3121 atcaatcagc tggcgctgca tgcgctcgat cgcgcggtag cgcgcgacga tgcgcgcgcc
     3181 gtagtcggtc agccgtgaac cgccgccgcg ctggccgccg gcgacgcgct cgaccagcgt
     3241 ctgcggcgcg aggttgttca tcgcgtcgat cgcatcccag gccgccttgt agctcatcgg
     3301 gatcgcgcgc gcggcggccg agatcgagcc gctgtgctgg atgtgttcga gcagtgcgac
     3361 gcgctcgggc ttcagcagcg tctgcccgtc gcgcgcgaag aaggcgagct gcgtggcgat
     3421 gtcggccggg gacggaggat cgatcgggct catgggcttg agagtcgcca ctgtacttcg
     3481 ctatatattt tgattcatat aaagtctgcc gcgtccggcc agccgaccgg gccatccctg
     3541 ccgtccgtca ccgaggtccg tcgccgtgtc cagcgcgtcg tccaccctgc cacgcccgcc
     3601 gctgaaggtc agcgccggca accagattca cggtcatgtc gtcacggtcg agcgcggcgc
     3661 ggtcaacgcg gaggtgctga tcgacatcgg cggcggggcc tcgatggtgg cgatcgtcac
     3721 cgacgagagc atcgacgcgc tgacgctgcg tccgggccgg gaagtctatg cgctggtgca
     3781 ggcgtcgagc gtgatcttca cgaccgagcg catgcccttc gtctcggcgc gcaacgcgct
     3841 gtggggcgtc gtcgatgcgc tgcgcaccgg cgccgtcaac gacgagattt ccatgcgcct
     3901 cgacgccggc cagcgcgtca ccgcgacgct gacgcacacc agcgtccacg aactcgggct
     3961 ggcaccgggg cggcgcgcct gcgcgctgat caaggcttcg agcgtgctgc tcgccgtgcc
     4021 ggcctgaggg gccgcgcggc tgcggcgccg tgcatgcccc atgacgaaaa aaacccgaga
     4081 cagaagcccc cgaggagtcg tcgcgatgtc cttccgtcct tcgctgccgg gcctgcgcgc
     4141 cggcctctgt gcgctgctgc tgtcggtgct gtcgctgccg gtgcaggcgg ccgagcgctt
     4201 catcaccgtg gcctcgacca cctcgaccga gcagtccggg ctctttggtt acctgctgcc
     4261 gaagttcacc ggaaagaccg gcatcgaggt gcgcgtggtc gcggtcggca ccggtcaggc
     4321 gatcaagatc gcgcagcagg gcgatgccga cgtgctgttc gtgcaccacc agccctccga
     4381 ggagaagttc gtcgccgagg gctacggcgt gcagcgcttc ccggtcatgt acaacgactt
     4441 catcgtcgtc ggtcccggca ccgacccggc cgccatcgag ggcggacacg acgtggtcgc
     4501 ggccttcagg gccatcgccg gaaagcaggc tccgttcgct tcgcgcggcg acgattccgg
     4561 cacgcacaag gccgaacagg cgctgtggaa ggcggccggc atcgacgtga aggccgcttc
     4621 cggtagctgg tatcgcgaga ccggctcggg catggggccg acgctgaaca ccgctgccgg
     4681 cctgccggcc tacgcgctgg ccgaccgcgg cacctggctg tcgttcagga accgcgggcc
     4741 gctgaagatc gtcgtcgagg gcgacaagag gctgttcaac cagtacggca tcctgctggt
     4801 cagcccggcg aagttcccgc acgtcaggca ggccgacgcc caggccttca tcgactgggt
     4861 gatctcgccg gaaggccagc aggccatcgc cggctaccgg atcaacggcg agcagctgtt
     4921 ctttccggat tacggccgct gaggcgccgg cgcggggcga acttccgcgt ccgcgcgccg
     4981 tctgcctgcg cacgcggccg gcgcgctggg tgccggcgcg ccatacggag agagaatccg
     5041 ccatgcgcct cgatgccggc tgtgagctcg aatttgaaat cctcgaaccg acgccgatgc
     5101 tgctgatgct gcgtccgcgc agcggcgaag gtcagtgggt ggaagcggaa cgctacgcgc
     5161 tgcagccgcg cgtgccggcc agcgagtacg ccgatgcctg gggcaatctc tgccagcgca
     5221 tcgtggcccg gcccgggcgg ctgcacatct cgacgcaggt gcgcgtcgac accgatgaca
     5281 cgatcgcgct cgcgcccggg gcgccgcgca cgccggtcga tcagctgccg gtcgaggtca
     5341 tcggcttcct gctcgccagc cgctacgccg aatccgacgc gctcgccgat caggcgcgcg
     5401 acgtgacggc cggcgccacg cccggctatg accaggtcga ggcgatccgc gcctggatcc
     5461 accgcagctt cgaataccgc tacggcgtca gcaacgaatc gacctcggcg cgcgacacga
     5521 tccgcatgcg caagggcgtg tgccgcgact tcgcgcacgt cggcatcgcg ctgacacgcg
     5581 cgctcggcat cccggcccgc tacgtcgccg gctacctgca cgaactggag ccgatggacc
     5641 tgcacgcctg gttcgaggcg tatgtcgacg ggcgctggta caccttcgac gccacgcagg
     5701 ccgggccgcg cggcgggcgc gtgatcatcg gctacggccg cgatgcgctc gacgtggcgc
     5761 tggcgacccc gttcggcgac acgaagctcg acaacatgca ggcctgggtg cgggcggtgc
     5821 cggaagccgc cggctgagcg ggccgcaaac ggccgcgggc cctgcgccga tccggcgaca
     5881 gggcccgcgg tgtttccggt ggagtgcggg gaagcgcgta tccggcttcc cggtgaagat
     5941 cggcgcggaa aacctcacgc gccggcgccg gccggcgccg ggcttacgac cagtagcccg
     6001 gaatccacag ccagccgtcc ggacgctgct cccagcggcc cggcacccat tcggcactct
     6061 gctgcggcgg ctgctcccag gtgcccggcg tccacgccca ggcgccgttc tcccagctcc
     6121 agtggcccgg cacccagcgg tattccgggg ccggctggcc gaccggctgc tcgacgcgcg
     6181 gcggcggcgg cggcacgtcg gcggcgacca cggcgccggg cggcggcggg ggggtcattt
     6241 cggtgacggt cggcgctggt ggcggggtgg tggccgggcc gaccgagtgg cggctgaccg
     6301 tgact
//
LOCUS       DAOQJH010000041         6297 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_6976_length_6297_cov_4.302627, whole genome shotgun sequence.
ACCESSION   DAOQJH010000041 DAOQJH010000000
VERSION     DAOQJH010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 6297)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6297)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6297
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6976_length_6297_cov_4.302627"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1994)
                     /locus_tag="PLQ18_01775"
     CDS             complement(<1..1994)
                     /locus_tag="PLQ18_01775"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HNE00407.1"
                     /translation="MFGLGKTRPGGGGSAAMAGEVARWDADGALWHLGSRRTGLSETE
                     AATRQRMYGDNRITQAPRTPLWRRLLQEFGNFFARVLWAAAALAFLAESQTPGQGMRE
                     LGFAIVAVILINGVFSFWQVQRAAQALAALQALLPQQVTLLRDGHCERRDSVLLVPGD
                     VIRLGAGERVPADCRLIEACDLRVSLATLSGESAPLTRASAACAQAHPLEADNLLLAG
                     TLLLSGHGTALVYATGMDTEFGRIAGLTQAAEDSASPLQREIAHISRLVTLLATALAV
                     GFFAIGQWLELPLHTSLMFAIGILVANVPEGLLPTVTLALAMATQRMARRRALVRSLP
                     AVEALGSTTVICSDKTGTLTENRMRVCRAATVAGVLTEGEALPGPLAEIARCCHDLQR
                     QADGRMIGDPLEQALHAWAGAGPGCRRIDERAFDAVRRRMSVLVARAGGERWLLCKGA
                     PETVLPRCRQAWTPAGIITLSHSERSALVRLQSAWADAGQRVLVLAWRPHDDTHIGEE
                     DGLILAGLVGLEDPPRPQVPEAIARCRAAGVRVIMVTGDHPQTALAIAREIGLVSSER
                     PVVLQGDGLVRMSAAQRARALAAPEVICARVSAEQKLLVVEALQAGGEVVAVTGDGVN
                     DAPALKRADVGIAMGIAGTDVAREAADIVLLDDHFATIVDA"
     gene            complement(2220..2669)
                     /locus_tag="PLQ18_01780"
     CDS             complement(2220..2669)
                     /locus_tag="PLQ18_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011189107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HNE00408.1"
                     /translation="MNLVKWDPLRDIEDVTTRVIDLLANPLRRRDGGRESLTVADWAP
                     AVDISETAEAFCLKVELPDVSKEDVHVTLDGDLLTIAGERRQTQTGKDTLVHRVECSY
                     GAFWRSFHLPSPIDAGAIDAQFKDGMLTVKLPKTEVKAVKGIEISIH"
     gene            complement(2792..3736)
                     /locus_tag="PLQ18_01785"
     CDS             complement(2792..3736)
                     /locus_tag="PLQ18_01785"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNE00409.1"
                     /translation="MCPEAAPEAAPSDPDGREAWELGWRRLLDSFGREPAEVAFVFER
                     DGRCRLATTGRAFSCLGQDDGRDWRVRLPEPVRAVIEQLAHLARLGVSTSRGVCCNGR
                     QYQFRSLALRPSEPAPGTVLVLGQDVTEANAAMQALHVLGSRLLGLAERDVLLDIANR
                     RCFERSLQREWRRLRREGGHLAVLFVDLDDFKACNDRFGHAWGDVCLLRVATVLQQVA
                     TRPADLVARYGGDEFAVLLPDTDEAGARLLAGQMHALLQGQQDPQHPRLTLTIGAAAA
                     EAGQLAMASELLDLADRALYRGKTRGRNCTVAASELAG"
     gene            complement(3829..4332)
                     /locus_tag="PLQ18_01790"
     CDS             complement(3829..4332)
                     /locus_tag="PLQ18_01790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE00410.1"
                     /translation="MTITPKPWFTYYDLGVDLQRCFEDNCTRLYQTARRSLSDEDGRE
                     PPARWWQAGLGCLGALRPWLLGWQSMADEQGEYLATALLRCQRQLGEQRERLAEEMLS
                     VWLAGLQRLEPPGPFALLDQLLGLPAASAKQQLPVVAKPGENPPPSESGAQASPAGKS
                     RASAVTS"
     gene            complement(4342..5124)
                     /locus_tag="PLQ18_01795"
     CDS             complement(4342..5124)
                     /locus_tag="PLQ18_01795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="PEP-CTERM proteins occur, often in large numbers,
                     in the proteomes of bacteria that also encode an
                     exosortase, a predicted intramembrane cysteine proteinase.
                     The presence of a PEP-CTERM domain at a protein's
                     C-terminus predicts cleavage within the sorting domain,
                     followed by covalent anchoring to some some component of
                     the (usually Gram-negative) cell surface. Many PEP-CTERM
                     proteins exhibit an unusual sequence composition that
                     includes large numbers of potential glycosylation sites.
                     Expression of one such protein has been shown restore the
                     ability of a bacterium to form floc, a type of biofilm;
                     Derived by automated computational analysis using gene
                     prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HNE00411.1"
                     /translation="MLRINRLAAALLAAGLLAGASGASATQIININSGSQWLSTTDAL
                     VATQWFHGNFTVGADGVPATAPYPNLVTTPNDIVPGSSAQLMWACGPGSTLCDNTGTN
                     GPTEVWFGMPLDLKANPIARLGAAQIIADDYFEFIVNGQFVQTGYLNDHQNPVSGQPV
                     PIGIDFTSYLHDGYNWLMIHALDGYFPTLGAACDPGYTDRPVQDARGTFCAGDRVGEY
                     AFVDGVIVSVPVPMTAALMGAGLLGLVRRRNRPATGAMRLMA"
     gene            complement(5814..>6297)
                     /locus_tag="PLQ18_01800"
     CDS             complement(5814..>6297)
                     /locus_tag="PLQ18_01800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE00412.1"
                     /translation="VLLIENWEAFDALDALRLDLSAAGADALVVWRGGGEGCRSDHAL
                     ALLAALGRPVLAFVDYDPAGLLIALTLPHLAGVLAPPGAELAERLRGGLRERYLEQLP
                     QCAATLDAAVQPDVARLWRLIRSAGRALPQEAFIEPEHTAQAGTAQASFPRLPASSDG
                     "
BASE COUNT          967 a         2286 c         2121 g          923 t
ORIGIN      
        1 gcatcgacga tggtcgcgaa gtggtcatcg agcagcacga tgtcggccgc ctcgcgcgcc
       61 acgtcggtgc cggcgatgcc catcgcgatg ccgacatcgg cgcgcttgag ggccggcgca
      121 tcgttgacgc cgtcgccggt gacggcgacg acctcgccgc cggcctgcag cgcctcgacg
      181 accagcagct tctgctcggc gctgacgcgc gcgcagatca cctcgggcgc ggcgagcgcc
      241 cgggcacgct gcgcggcact catgcgcacg agcccgtcgc cctgcagcac gaccggccgc
      301 tcgctgctca ccaggccgat ctcgcgggca atcgcgagcg cggtctgcgg gtggtcgccg
      361 gtgaccatga tcacgcgcac gccggcggcg cggcagcggg cgatggcctc gggcacctgc
      421 gggcgcggcg gatcctccag gccgacgagg ccggcgagga tcaggccgtc ctcctcgccg
      481 atgtgcgtgt cgtcgtgcgg gcgccaggcc agcaccagca cacgctggcc ggcatcggcc
      541 caggccgact gcaggcgcac cagcgccgag cgttcgctgt ggctgagcgt gatgatgccc
      601 gccggcgtcc aggcctgccg gcagcgcggc agcacggttt cgggcgcgcc cttgcagagc
      661 agccagcgct cgccgccggc gcgcgcgacc agcaccgaca tgcgccggcg cacggcgtcg
      721 aaggcccgct cgtcgatgcg ccggcagccc ggtccggccc cggcccaggc gtgcagcgct
      781 tgctccagcg gatcgccgat catgcggcca tcggcctgcc gctgcaggtc gtggcagcag
      841 cgcgcgatct cggccagcgg ccccggcagc gcttcgccct cggtcagcac gccggcgacg
      901 gtggccgcgc ggcacacgcg catgcggttt tcggtcagcg tgccggtctt gtccgaacag
      961 atcaccgtcg tcgagccgag cgcctcgacc gccggcaggc tgcgcaccag cgcccggcgc
     1021 cgcgccatgc gctgcgtggc catggcgagc gccagcgtca ccgtcggcag caggccctcg
     1081 gggacgttgg cgaccaggat gccgatggca aacatcaggc tggtgtgcag cggcagctcc
     1141 agccactggc cgatggcgaa gaagccgacc gcgagcgcgg tggcgagcag cgtgaccagc
     1201 cgcgagatgt gcgcgatctc gcgctgcagc ggcgaggcgc tgtcttcggc cgcctgcgtc
     1261 aggccggcga tgcgcccgaa ctcggtgtcc atgccggtcg cgtacaccag ggccgtgccg
     1321 tggccgctga gcagcagcgt gccggcgagc agcaggttgt cggcctccag cggatgcgcc
     1381 tgcgcgcagg ccgcgctggc gcgggtcagc ggtgccgatt cgccgctcag cgtcgcgagg
     1441 ctcacgcgca ggtcgcaggc ttcgatcagc cggcagtcgg ccggcacccg ctcgccggcc
     1501 ccgaggcgga tcacgtcccc cggcaccagc agcacgctgt cgcgccgctc gcagtgcccg
     1561 tcgcgcagca gcgtcacctg ctgcggcagc agcgcctgca gcgcagccag cgcctgcgcg
     1621 gcgcgctgca cctgccagaa cgagaacacg ccgttgatca ggatgacggc gacgatggcg
     1681 aagccgagtt cgcgcatgcc ctggccgggc gtctggctct ccgccagaaa cgccagcgcc
     1741 gccgccgccc acagcacccg cgcgaagaaa ttgccgaact cctgcagcag ccgccgccag
     1801 agcggcgtgc gcggagcctg cgtgatgcgg ttgtcgccgt acatccgctg ccgcgtcgcg
     1861 gcttccgtct cggaaagccc cgtgcgccgg ctgcccagat gccagagcgc cccgtccgcg
     1921 tcccagcgtg cgacctcgcc ggccattgcc gcgctgccgc cacctcccgg gcgcgtcttt
     1981 cccagcccga acatgtctca tgcccccgcc cgcatgcagg cttcaaaaaa gaaaggccgg
     2041 tgccgccgcg tctccgggca ccggccgaat cagggacctt ttcgtccccc ggataccggt
     2101 cgccgcccgc atggcgaccg gactcactgg cgacacgctg ccgcccggca ctcgggagcc
     2161 cctccccccg aagagcgctc cgccccgagg ccgggtgggc gtgcaccgtt gtcatccagt
     2221 caatggatgc tgatctcgat gcccttgacc gccttcacct ccgtcttcgg cagcttcacc
     2281 gtcagcatgc cgtccttgaa ctgcgcatcg atcgcgccgg catcgatcgg actcggcagg
     2341 tggaagctgc gccagaacgc gccgtagctg cactccacgc gatgcacgag cgtgtccttg
     2401 ccggtctgcg tctggcgccg ctcgccggcg atcgtcagca ggtcgccgtc gagcgtcaca
     2461 tgcacgtctt ccttgctgac gtccggcaac tcgaccttca ggcagaaggc ttcggccgtc
     2521 tcgctgatgt cgacggccgg cgcccagtcg gcgacggtca ggctttcccg tccgccatcg
     2581 cgtcggcgca gcggattggc cagcagatcg atcacccggg tggtgacgtc ctcgatgtcg
     2641 cgcagcggat cccacttgac caggttcatc ggataccccc tcggaaacct catgcggacc
     2701 gcaggtctgc cggcagtccc ccggcccgcc ggactcggga ctgccggcac gcaccggcga
     2761 cggcaggcac tccgtcgccg cttgcatcgg ttcagcccgc aagctccgaa gccgcaaccg
     2821 tgcagttgcg cccccgggtc ttgccgcgat acagcgcccg atcggcgaga tcgagcagtt
     2881 cgctcgccat cgccagctgc cccgcctccg ccgcggccgc gccgatggtc agcgtcagcc
     2941 gggggtgctg cgggtcctgc tggccctgca gcagcgcgtg catctgcccg gcgagcagcc
     3001 gggcgccggc ctcgtcggta tcgggcagca gcacggcaaa ctcgtcgccg ccgtagcggg
     3061 ccaccagatc ggccgggcgg gtcgcgacct gctgcagcac ggtggccaca cgcagcaggc
     3121 agacatcgcc ccaggcatgg ccgaagcggt cgttgcaggc cttgaagtcg tcgagatcga
     3181 cgaacagcac ggccaggtgg ccgccctcgc gccggagccg gcgccattcc cgctgcaggc
     3241 tgcgctcgaa gcagcgccgg ttggcgatgt cgagcagcac gtcgcgctcg gccagcccga
     3301 gcagccggct gccgagcacg tgcagggcct gcatcgcggc gttggcttcg gtcacgtcct
     3361 gccccagcac cagcaccgtt ccgggcgccg gttcgctggg gcgcagcgcc aggctgcgga
     3421 actggtactg ccgtccgttg cagcacacgc cgcggctcgt cgagaccccg agccgggcca
     3481 gatgcgcgag ctgttcgatg actgcgcgca ccggctccgg caaccgcacc cgccagtcgc
     3541 ggccatcatc ctggcccaga caggaaaacg ccctgccggt ggtcgccagc cggcagcggc
     3601 cgtcgcgctc gaacacgaac gccacctccg cgggctcgcg cccgaagctg tcgagcagtc
     3661 gccgccagcc aagctcccag gcttcgcggc cgtcgggatc ggacggtgcc gcctccgggg
     3721 ccgcttcagg gcacaccttc ccggccgcca ccccggtaca accggtgccg ctcccggtac
     3781 cacgcacaca ggttgcgctg ccagcgcccc ggccaggggc gggccatgtc agctggtgac
     3841 cgcgctggcg cggctctttc cggcagggct ggcctgcgct ccggattcgg acggcggcgg
     3901 gttttccccg ggcttcgcca ccacgggcaa ctgctgcttc gcactcgccg ccggcaaacc
     3961 cagcagctga tcgagcagtg cgaacggccc cggcggttcc agtcgctgca gaccagccag
     4021 ccagacgctg agcatctctt cggcaagccg ctcgcgctgc tcgccgagct ggcgctggca
     4081 gcgcagcagc gccgtcgcca gatattcgcc ctgctcatcg gccatcgact gccagccgag
     4141 cagccacggc cgcagcgccc cgaggcagcc gagccccgcc tgccaccacc gggccggcgg
     4201 ctcgcgcccg tcctcgtcgc tcaggctccg gcgggcggtc tgatagaggc gtgtgcagtt
     4261 gtcttcgaag cagcgctgca ggtcgacgcc caggtcgtag tacgtgaacc agggtttcgg
     4321 ggtaatagtc atgggacctc ctcaggccat caggcgcatc gcgcccgtcg ccggccggtt
     4381 gcgccgacgc accagaccga gcagcccggc gcccatcagc gccgcggtca tcggcaccgg
     4441 caccgaaacg atgacgccat cgacgaaggc gtactcgccg acacgatccc cggcgcagaa
     4501 cgtgccgcgg gcatcctgaa ccgggcggtc ggtgtagccg ggatcacagg ccgcacccag
     4561 ggtcgggaag tagccatcga gggcatggat catcagccag ttgtagccat cgtgcaggta
     4621 actggtgaaa tcgatgccga tcggcaccgg ctggccgctg accggattct ggtgatcatt
     4681 cagatagccg gtctgcacga actggccgtt gacgatgaat tcgaagtagt cgtcggcaat
     4741 gatctgcgcc gccccgagcc gggcgattgg attcgccttc aggtcgagcg gcatgccgaa
     4801 ccagacctcg gtcggcccgt tggtgccggt gttgtcacag agcgtgctac ccggcccgca
     4861 ggcccacatc agctgcgccg agctaccggg cacgatgtcg ttcggcgtgg tgaccagatt
     4921 cggatacggc gccgtcgccg gcacaccatc tgctcccacc gtgaagttgc cgtggaacca
     4981 ttgggtggca accagcgcgt cggtggtcga caaccactgg ctgccgctgt tgatgttgat
     5041 gatctgcgtg gccgaggcac cagaggcgcc agcgaggagg ccggcggcga gcagtgccgc
     5101 ggcaagacgg ttgatcctga gcatcggaac cccctgaatc cggattgacc catgccgatt
     5161 cgatatcggc agcttgcttg tgccctccgt gaacaggcgg tcggcccatc attgtttccc
     5221 tgcacccctg tggccggtaa gaccaatcta tacctgtgag acacatattg cattaaacac
     5281 aaaaaaatag tgtggataca tttctgcatt tctgaactgc atattgaaat gccatgcatg
     5341 catcatgccg tccacataat tttcttgctt tttattaact tgaaaatttg agcgtcatat
     5401 tcgtcaccat gaatacagct tgacttatcc tgttttcagg aaaacaagca ggcaggcgct
     5461 gcgggtctat ctgacgcccg gaccgactga agtgaggcga acctccgtgc caggcgcgtt
     5521 tcatccgcaa gcgtgaggca agagaagatg gattcctgct cgcaggtgca acccggaatc
     5581 gccatcgaaa gccatgcgat ttgcgcatgg atgaaccctc ttccgatcac ccggacacgc
     5641 ctctgccggc agtcatcgac gcgatacgca gtgcctgcat gaggggactg ccgggaatcg
     5701 gcgcacagcg cggtccgggc ctgtgcggca ggaggaaagc cggcatcggg agggtttgcc
     5761 ggtccgcggg ccggcctcgt ggctggcggg gatcggccct tggggtcgaa ggctcagccg
     5821 tcggaggaag ccggaagccg ggggaaggag gcttgcgcgg tgccggcttg cgcggtgtgc
     5881 tccggctcga tgaacgcctc ctgcggcagc gcgcgcccgg cgctgcggat cagccgccac
     5941 aaccgcgcca cgtccggctg cacggcggca tcgagcgtcg ccgcgcactg cggcagttgt
     6001 tcgagataac gctcgcgcag gccgccgcgc aggcgctcgg cgagttcggc cccgggcggc
     6061 gcgaggacgc ctgccagatg cggcagcgtc agcgcgatca gcaggccggc cgggtcgtag
     6121 tcgacaaaag cgagcaccgg ccgcccgagc gcggcgagca gcgccagcgc gtgatcgctg
     6181 cgacagccct cgccgccgcc gcgccagacc accagcgcat ccgcaccggc ggcgctcaga
     6241 tcgagccgca gcgcgtcgag cgcatcgaag gcttcccagt tctcgatcag cagcacg
//
LOCUS       DAOQJH010000042         6282 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_6995_length_6282_cov_4.761844, whole genome shotgun sequence.
ACCESSION   DAOQJH010000042 DAOQJH010000000
VERSION     DAOQJH010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 6282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6282
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6995_length_6282_cov_4.761844"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1783)
                     /locus_tag="PLQ18_01805"
     CDS             complement(<1..1783)
                     /locus_tag="PLQ18_01805"
                     /EC_number="6.2.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018991011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030729 - acetoacetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate--CoA ligase"
                     /protein_id="HNE00413.1"
                     /translation="MTDIPLWQPAPKRIARANLTAFRTAVNAAHGLDLPDYPALYDWS
                     IREPEAFWRTLWDWADVIGERGERTLTGAGSMPGAQWFPDAQLNFAENLLRRNDDSTA
                     IVFRGEDKVEKRLSWRELNAAVSRLAQALLNERVQPGDRVAAFLPNLPETVIGMLAAT
                     SIGAVWSSCSPDFGTQGVLDRFGQIEPVVLIGCDGYWYNGKRIDTLHKLGEIRAQLPS
                     VRRLVVVGYLDAIPEVSAIPDAVRLDEFIAHWPAHTPEFRRQPFSHPLYIMFSSGTTG
                     VPKCIVHGAGGTLLQHLKEHRLHTDVRPGERVFYYTTCGWMMWNWLVSALASEATLLL
                     YDGSPFAPDGNVLFDYAQAERMNVFGTSAKYIDACAKAGIAPIDTHALPELRAILSTG
                     SPLVPESFDYVYARVKSDVQLSSISGGTDIVSCFALGNPVLPVWRGELQCRGLGLKVE
                     VWNDDGQPVRGEKGELVCTAPFPCMPIGFWNDPEGVKYRAAYFERFPGIWCHGDYVEL
                     TAHDGLIIYGRSDAVLNPGGVRIGTAEIYRQVEQLDEVVESLVIGQDWHGDVRVVLFV
                     HLRPGVTLDDALQKKIRDHIRARTTPRH"
     gene            complement(1930..2835)
                     /locus_tag="PLQ18_01810"
     CDS             complement(1930..2835)
                     /locus_tag="PLQ18_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004315436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HNE00414.1"
                     /translation="MSLPESVKIVEVGPRDGLQNEAATVPTEIKVELIERLTAAGLPA
                     IEATSFVSPKWVPQMADSAEVLARLRRPPGCSYPVLTPNLKGFEAALAAGAREVAVFA
                     AASETFSQRNINCSIAESLARFEPLLAAAEAAGVRVRGYVSCVAGCPYEGAVAPSAVA
                     RVALELLRMGCYEISLGDTIGIGTPATMLAMLDAVGGAVSPARLAVHCHDTRGMGIAN
                     VYACVQAGITVVDASVAGLGGCPYAPGASGNVATEDVVWLLDGLGVHTGVDLVKLAET
                     GQWISDRLGRPNGSRAGRALLALRH"
     gene            complement(2960..4192)
                     /locus_tag="PLQ18_01815"
     CDS             complement(2960..4192)
                     /locus_tag="PLQ18_01815"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HNE00415.1"
                     /translation="MSTPPLIARQPVYDARHHVLAWDIAHARAQHPAATPPAEPDSEA
                     AEAAHFELLTGALLVAGLERLIGNGFGFLPVSAHTLLSPTLRLLPPLQVVLTLPAAGL
                     QSVDLPARLAPLQRAGYRVALDGCRSLEPGLLPLLPDLDFLRLDAAALGAAALAPLLQ
                     SLRGAGFHGVAIATGIADAGTAQAALDAGVLGLQGPLFRRPQPVAASALDTGREAALT
                     LIAALNRPDAGLPEISNLIRRDATLTYKLLRYVGSAWFGLGEVHSVDHALICLGRLRL
                     RLWATLVAMTGAAGERHDLLELALVRAGMCRDLLLAEGGDAETGTLAGLLSTLDLLFG
                     LPLERVLAGLPLNRELRQALLEHAGPAGRALALALAYERGDWEEVHAAGRDEQVFDAY
                     FAALAQAHETLEQIGTSH"
     gene            4604..5686
                     /locus_tag="PLQ18_01820"
     CDS             4604..5686
                     /locus_tag="PLQ18_01820"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HNE00416.1"
                     /translation="MSASALLTRCLPGLALAAGLALGSATAAAAPYTDFYVFGDSLSD
                     TGNVALVSGGTVPPAPYFNGRFSNGPNWADRFAAEGSLFTSTQTPLLPGSVLGNVGDN
                     YAWGGARTYGHSNPALNSIARLSSQIGAFASQHAGVADPDALYVIWIGANDMQDAVVA
                     AATAYQNALIGGATPAAAQLIALPLINSAVTVAISNINSAISSLLALGADEFLVANLP
                     DLSLTPRLAPAGLFAQYASTSFNAALLALLQTRPGTAHLADIQSAYNELLGNPAAYGF
                     TNTTAPCYTGDDITFTPNTGTVCANPDQYVFWDTFHPTAAASAWIEAEMVSALAATIP
                     EPASYALLGAGLLAFGRARRQAGRRD"
     gene            5870..6223
                     /locus_tag="PLQ18_01825"
     CDS             5870..6223
                     /locus_tag="PLQ18_01825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE00417.1"
                     /translation="MIRTSFRNEFRNNLRKGLRTVLIACPLSAGLAFASGAAFAAGQP
                     AHGHGSPVQLADAHGSRPLPVYGRAPMTADACHGASPRGCRDDAEAGRREAVQPLLCL
                     GASPRGCLLRPASEV"
BASE COUNT          934 a         2217 c         2238 g          893 t
ORIGIN      
        1 cgtggcgcgg cgtggtgcgg gcgcggatgt ggtcgcggat cttcttctgc agggcgtcat
       61 cgagcgtgac gccggggcgc aggtgcacga acagcacgac gcgcacgtcg ccgtgccagt
      121 cctggccgat caccagactc tcgacgacct cgtcgagctg ttcgacctgg cggtagatct
      181 cggccgtgcc gatgcgcacg ccgccggggt tcagcacggc atcggaacgg ccgtagatga
      241 tcaggccgtc gtgcgcggtc agttcgacgt agtcgccgtg acaccagatg ccggggaagc
      301 gctcgaagta cgcggcccgg tacttcacgc cttcggggtc gttccagaag ccgatcggca
      361 tgcacgggaa gggcgcggtg cagaccagct cgcccttctc gccgcgcacc ggctggccgt
      421 cgtcgttcca gacctcgacc ttcagcccga gcccgcggca ctgcagctcg ccgcgccaga
      481 ccggcagtac cggattgccg agcgcgaagc acgagacgat gtcggtgccg ccggagatcg
      541 agctcagctg cacgtcggac ttcacgcgcg cgtagacgta atcgaagctc tccggcacca
      601 gcgggctgcc ggtcgagagg atcgcgcgca gctcgggcag cgcgtgcgtg tcgatcggcg
      661 cgatgccggc cttggcgcag gcgtcgatgt acttcgccga ggtgccgaac acgttcatgc
      721 gctcggcctg cgcgtagtcg aacagcacgt tgccgtcggg cgcgaacggc gagccgtcgt
      781 agagcagcag cgtcgcctcg ctcgccagcg cgctgaccag ccagttccac atcatccagc
      841 cgcaggtcgt gtagtagaac acccgctcgc cggggcgcac gtcggtgtgc agccggtgct
      901 ccttcaggtg ctgcagcagc gtgccgccgg cgccgtgcac gatgcacttc ggcacgccgg
      961 tcgtgccgga gctgaacatg atgtacagcg gatggctgaa gggctggcgc ctgaattccg
     1021 gtgtgtgcgc cggccagtgc gcgatgaatt catcgaggcg cacggcatcg ggaatcgccg
     1081 agacctcggg gatggcatcg aggtagccga ccacgaccag ccggcgcacc gagggcagct
     1141 gcgcgcggat ctcgccgagc ttgtgcaggg tgtcgatgcg cttgccgttg taccagtagc
     1201 cgtcgcagcc gatcagcacg accggttcga tctggccgaa acgatcgagc acgccctgcg
     1261 tgccgaaatc cggcgagcac gaggaccaga ccgcgccgat cgaggttgcc gccagcatgc
     1321 cgatgacggt ttccggcagg ttgggcagga aggccgcgac gcgatcgccc ggctgcacgc
     1381 gctcgttgag cagcgcctgc gccagtctgg acaccgcggc attcagctcg cgccagctca
     1441 ggcgcttttc gaccttgtcc tcgccgcgga acacgatcgc ggtgctgtcg tcgttcctgc
     1501 gcagcaggtt ctcggcgaag ttcagctgcg catccggaaa ccactgcgcg ccgggcatgg
     1561 agccggcgcc ggtcagcgtg cgctcgccgc gctcgccgat cacgtcggcc cagtcccaca
     1621 gcgtgcgcca gaaggcttcg ggctcgcgga tcgaccagtc gtacagcgcc gggtaatccg
     1681 gcaggtcgag gccgtgggcg gcgttcacgg cggtgcggaa ggcggtcagg ttggcgcggg
     1741 cgatgcgctt cggagcgggc tgccacagcg ggatatcggt catgggcggg gactccgggg
     1801 acaggatcgg ggaatcggga gcaggcggcg gccacggggc ggcaggcgcg gcagcgcggc
     1861 gccgggccgg tggcagcgac ttgccacgtg gcaagtccgg cgaccggccg gcggggcggc
     1921 tgcgggcggt cagtggcgca gggcgagcag cgcgcggccg gcgcgcgagc cgttcggccg
     1981 gccgagccgg tcgctgatcc actggccggt ttcggcgagc ttcacgagat cgacgccggt
     2041 atgcacgccg aggccgtcga gcagccagac gacatcctcg gtcgcgacgt tgccgctcgc
     2101 gcccggtgcg tacgggcagc cgccgaggcc ggcgaccgag gcatcgacga ccgtgatgcc
     2161 ggcctgcacg caggcgtaga cgttggcgat gcccatgccg cgcgtgtcgt gacagtgcac
     2221 ggccagccgc gccggcgaca ccgcgccgcc gacggcatcg agcatcgcca gcatcgttgc
     2281 cggcgtgccg atgccgatgg tgtcgccgag cgagatctcg tagcagccca tgcgcagcag
     2341 ttcgagcgcg acgcgcgcga ccgccgaggg cgccaccgcg ccttcgtacg ggcagccggc
     2401 gacgcaggag acgtaaccgc gcacgcgcac gccggcggcc tcggcggcgg cgagcagcgg
     2461 ctcgaagcgc gccagcgact cggcgatcga acagttgatg ttgcgttgcg agaaggtctc
     2521 ggaggcggcc gcgaacaccg cgacctcgcg cgccccggcg gcgagcgcgg cctcgaagcc
     2581 cttgaggttc ggcgtcagca ccggatagct gcagcccggc ggccggcgca ggcgcgccag
     2641 cacctcggcg ctgtcggcca tctgcggcac ccacttcggc gacacgaaac tggtcgcctc
     2701 gatcgccggc aggccggcag cggtcaggcg ctcgatcagc tcgaccttga tctcggtcgg
     2761 caccgtggcc gcctcgttct gcaggccgtc gcgcggaccg acctcgacga tcttcacgga
     2821 ctcgggcagg gacatcggca cacctcggcg attcgcggga ttcacgcagg gacgtgaatc
     2881 tcttgtagcc ggttccgtgc cgggcggccg ccttgcagtg cagcgacgcc gtgtttcagg
     2941 gacgatccgg gatggaggat cagtggctgg tgccgatctg ctccagcgtc tcgtgcgcct
     3001 gtgcgagcgc ggcgaagtag gcatcgaaca cctgctcgtc acggccggca gcgtggacct
     3061 cctcccagtc gccgcgctcg taggcgagcg cgagcgccag cgcccggccg gccgggccgg
     3121 cgtgttcgag cagggcctga cgcagctcgc gattgagcgg caggccggcg agcacacgct
     3181 ccagcggcag tccgaacagc aggtcgagcg tcgacagcag gccggcgagc gtgccggtct
     3241 cggcatcgcc gccctcggcg agcagcaggt cgcggcacat gccggcgcgc accagcgcca
     3301 gttcgagcag gtcgtggcgc tcgccggcgg cgccggtcat cgcgaccagc gtcgcccaca
     3361 gccgcagccg cagccgcccg aggcagatca gcgcgtgatc gaccgagtgc acctcgccga
     3421 gcccgaacca ggccgagccg acgtagcgca gcagcttgta ggtcagcgtc gcgtcgcggc
     3481 ggatcaggtt gctgatctcg ggcaggccgg catcggggcg gttcagcgcc gcgatcagcg
     3541 tcagcgccgc ttcgcgaccg gtatcgagtg cgctggccgc gaccggctgc ggccggcgga
     3601 acagcggtcc ctgcaggccg agcacgccgg catcgagcgc ggcctgcgcg gttccggcat
     3661 cggcgatccc ggtcgcgatc gccacgccgt ggaaaccggc accgcgcagc gactgcagca
     3721 gcggtgccag tgcggcggca ccgagcgcgg cggcatcgag gcgcaggaag tcgagatccg
     3781 gcagcagcgg caggagcccg ggttcgaggc tgcgacagcc atcgagcgcg acgcggtaac
     3841 cggcacgctg cagcggcgcc agccgggccg gcaggtcgac cgactggaga ccggcggccg
     3901 gcagcgtcag caccacctgc agcggcggca gcaggcgcag cgtcggactg agcagcgtgt
     3961 gcgccgaaac cggcaggaag ccgaagccgt tgccgatcag ccgttcgagt ccggcgacca
     4021 gcagcgcacc ggtcagcagc tcgaagtgcg cggcttcggc tgcctcggag tccggctcgg
     4081 cagggggtgt ggccgccggg tgctgtgccc tggcatgggc aatgtcccag gcgagcacat
     4141 ggtgtcgggc gtcatacacc ggttgacggg caatcaacgg gggcgtgctc atggcggcat
     4201 tccagcggct gtttgaacgt cggggggacc gccggcagcg caccggcggc cacggcccga
     4261 tcatggcggg aaataccaca tcgacgaccc gtccattacg actattccgg atggaaacgg
     4321 gatcggaaaa tccagtcgca atgcggcttc ggcgcaatgc agcacgtaat tcccgcgact
     4381 gcagcggggc gggtttgacg attgatgaca ataccgcagg cgcagatgca tgccggcggc
     4441 ctggctgccg ccgaaaccgg gctgcggcac ttcaggacat ccgttgctgt gctagtcctg
     4501 tgaagccccg cgcttccttg cagcccccgc cggccgtgtc gcgtcgcccc gtcgaccgca
     4561 gcggaggggc tgcgtccgaa acccgtccag aggttgccct gatatgtccg catctgccct
     4621 tctcacccgc tgcctgcccg ggcttgcgct ggctgccggt ctcgcactcg gcagcgccac
     4681 ggccgccgcc gcgccgtata cggatttcta cgtgttcggc gacagcctct ccgataccgg
     4741 caacgtcgcg ctggtctcgg gcggcacggt gccgccggcg ccgtacttca acggccgttt
     4801 ctcgaacggt ccgaactggg ccgaccggtt cgcggccgaa ggcagcctgt tcaccagtac
     4861 gcagacaccg ctgttgcccg gctcggtgct cggcaacgtc ggggacaact acgcctgggg
     4921 cggcgcacgc acctacgggc attcgaaccc ggcgctcaat tcgatcgccc ggctcagctc
     4981 gcagatcggc gccttcgcga gccagcacgc cggcgtcgcc gatccggacg cgctgtacgt
     5041 gatctggatc ggcgccaatg acatgcagga tgcggtcgtc gccgccgcga ccgcctacca
     5101 gaacgcgctg atcggcggcg cgacgccggc cgccgcgcag ctgatcgcgc tgccgctgat
     5161 caacagtgcc gtcacggtcg cgatcagcaa catcaattcg gcgatcagtt cgctgcttgc
     5221 cctcggcgcc gatgaattcc tcgtcgccaa cctgccggac ctgtcgctga cgccgcggct
     5281 ggcgccggcc gggctgttcg cgcagtacgc atcgacgagc ttcaacgccg cgctgctcgc
     5341 gctgctgcag acccggcccg gcaccgcgca tctggccgac atccagtcgg cctacaacga
     5401 gctgctcggc aacccggccg cctacggttt caccaacacc acggcaccct gctacaccgg
     5461 cgacgacatc accttcacgc ccaacaccgg cacggtctgc gcgaacccgg accagtacgt
     5521 gttctgggac accttccacc cgaccgctgc cgccagcgcc tggatcgagg ccgagatggt
     5581 ctcggcactg gccgcgacga ttccggagcc ggcctcgtat gcgctgctcg gcgccgggct
     5641 gctggccttc ggcagggcgc gccggcaggc cgggcgccgg gactgatccc cgcgttccgg
     5701 gcgatcgaac cagaacgccg gcgtccgcgt gacgccggcg ttttttattt gcggatggtc
     5761 tgctgcgagc tgtctccgcg gcgcgggcgc cggcgtatcg ctcttcaagg aagctcgatc
     5821 gaagcttccg caacccgacg atacccgcct gtttcacgga gatgccgcca tgatccgcac
     5881 aagcttccgc aatgaattcc gcaataacct ccgcaagggc ctgcgcaccg tcctgatcgc
     5941 ctgcccgctg tcggccgggc tcgcgttcgc cagcggtgct gcgttcgccg ccggccagcc
     6001 ggcccacggc cacggctcgc cggtgcagct cgccgatgcg cacggcagcc ggccgctgcc
     6061 ggtgtacggc cgcgcgccga tgacggccga tgcctgccac ggtgcctcgc cgcgcggctg
     6121 ccgcgacgac gccgaagccg gtcgccgcga ggccgtgcag ccgctgctct gcctcggcgc
     6181 ctcgccgcgc ggctgcctgc tgcgcccggc gagcgaggtg tgaaccggcc gccgcgctgg
     6241 tgccgccgcg accggcggtg gccgggggct cagaaggccg cc
//
LOCUS       DAOQJH010000043         6256 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_7044_length_6256_cov_4.135462, whole genome shotgun sequence.
ACCESSION   DAOQJH010000043 DAOQJH010000000
VERSION     DAOQJH010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 6256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6256
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7044_length_6256_cov_4.135462"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            437..1567
                     /locus_tag="PLQ18_01830"
     CDS             437..1567
                     /locus_tag="PLQ18_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003141690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HNE00418.1"
                     /translation="MKKAIQRAFAVSLLAGAVAFGVGAHAADVIKIGVAGPMTGGNAA
                     FGEQFWRGAVQAAEDINAAGGVNGKKIELVKADDACEPKQAVAVANRLVDQDKVDAII
                     GHFCSSSTIPASEIYAEAGKLQITPASTNPKVTERNIPTIFRTCGRDDQQGAVAADFI
                     LSTLKAKKIAVIHDKDTYGQGLADAMKASAAKKGAKEVLYEGLTRGEKDFNALATKIK
                     AADADAVYFGGLAAEAGPLVRQLREQGSTAAFISGDGIVDTAFVTAAGGPQFSKGVYM
                     TFGADPRNLPGGKSLVEKFRKAGYEPEGYTLYSYATLQAVTEAMKATKSTDGATLAKW
                     LKGHTVNTVMGPKAWDAKGDLKVSDYVMYQWDDKGGYAELKK"
     gene            1697..2614
                     /locus_tag="PLQ18_01835"
     CDS             1697..2614
                     /locus_tag="PLQ18_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013797474.1"
                     /note="LivHMGF is the membrane component of the LIV-I/LS
                     branched-chain amino acid transporter; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease LivH"
                     /protein_id="HNE00419.1"
                     /translation="MDWHILAQQLVNGLTLGSIYGLIAIGYTMVYGIIGMINFAHGDV
                     YMVSAYLTAIAIAVLTGFGIDSAPFAIVITLALTMFITGVYGWSIERVAYRPLRGSTR
                     LAPLISAIGISLVLQNYVQLSQGAKPQGVPTLVSGTVRLGTETNFVQMTYIQILIVVV
                     SFAAMAALTWIIRSTSLGRQCRATQQDRKMAAILGIDTDRIISTVFVIGAAMAAIAGV
                     LVTLNYGSFDFFIGFVAGIKAFTAAVLGGIGSLPGAMLGGILLGLFESLFSGFVNTDY
                     KDVFSFALLVLVLIFRPSGLLGRPEVEKV"
     gene            2619..3950
                     /gene="livM"
                     /locus_tag="PLQ18_01840"
     CDS             2619..3950
                     /gene="livM"
                     /locus_tag="PLQ18_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004946910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivM"
                     /protein_id="HNE00420.1"
                     /translation="MLPAAPLHSRIDVLASLREALLAFVLALVVFGPVSGLMLSGYKV
                     EPHWLRPLLLAAVVAAGRFAIHLLGQTPAGRRMVARNQARVHGGVTVAALGQSPNRAR
                     LLLVFVLLGAFVIPFLPWLPVPDALAALGGKYWLSNLILALIYVLLGLGLNIVVGLAG
                     LLDLGYVAFYAVGAYFLALGAQYLGIGFWTALVCAPLLAACCGAVLGFPVLRMHGDYL
                     AIVTLGFGEIIRLVLNNWLEFTGGPNGAPVPPPTLFGLEFTRTARQGGTPIHEFLGIE
                     YNTNLKLVFTYLVLLVVVILMVRLLTRLRQIPMGRAWEALREDEIACRSLGINHVVVK
                     LTAFMMGAMTGGLAGVFFATYQGFVNPTSFTFFESALILAIVVLGGLGSTVGVIIAAL
                     VLTLLPELLREFADFRVLAFGLLMVLMMIWRPRGLIRPQRPAFAPPQEVAR"
     gene            3947..4786
                     /locus_tag="PLQ18_01845"
     CDS             3947..4786
                     /locus_tag="PLQ18_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013662648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNE00421.1"
                     /translation="MKPSSLLEVRGLTMRFGGIVANNDVSFDVADGSITALIGPNGAG
                     KTTVFNCLTGFYRASGGTIHLATAAGAPVEVGSLLDKPFIGGSYLVARAGIARTFQNI
                     RLFREMSVVENLLVAQHQRLDRNVLAGALGTRKFRQTERDGLARAFHWLEVMGLTADA
                     NRLAGELPYGRQRRLEIARAMCTGPRLVCLDEPAAGLNPRETHELSELIRTLRSEHRV
                     TVLLIEHDMSLVMGISEHIVVLDHGEVIAAGNPETIRSSPAVIAAYLGAEEAEVEGGE
                     GTR"
     gene            4816..5532
                     /locus_tag="PLQ18_01850"
     CDS             4816..5532
                     /locus_tag="PLQ18_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009398629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNE00422.1"
                     /translation="MPQPLLDVRGIDTFYGAIQALRGVSLHVSRGEIVTLIGANGAGK
                     TTLLGSIFGNPRASVGSVSFDGEDITTLATHRIAPRGIALVPEGRHIFPAMTVEENLV
                     MGTIPVGEAHLDEDRAQMFELFPRLQERRNQRAGTLSGGEQQMLAIARALMSRPQLIL
                     LDEPSLGLAPLVVRRIFEVLREIASLGKTIFLVEQNANHALKLADRAYVLVNGEIRLQ
                     GSGRELLADPEVRKAYLGGH"
     gene            complement(5668..5910)
                     /locus_tag="PLQ18_01855"
     CDS             complement(5668..5910)
                     /locus_tag="PLQ18_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HNE00423.1"
                     /translation="MEAFLGQILLLPYTYAPSGWVRCEGQLLSIAQNEALYSLLGNQF
                     GGDGMRTFALPDLRDKEPAPNLHFCIAVQGIYPMRD"
BASE COUNT          930 a         2252 c         2054 g         1020 t
ORIGIN      
        1 gcctgaccac cgggccatgg cgaggcgcgc cggcggctct ctgcaccgtt gcggcgcagg
       61 gccggccgcg ggcgggggcg ctgcagcggc aagtttctga tcagccgcgc ttgcgccggg
      121 ctggcacgga aacggtattc cgcctgcgga atgtgccgct tcggggcgcc cgtgatctgc
      181 gtcagccaag ctgtccggtg tccggcccgc aggcttgaca gtcgcgtgac acggcagcca
      241 taagcactgc gcggaacagt ggcaccccca gaacgtgccg gccggcgcag gacgccggta
      301 ccggattccg cccgcgcccg cgacggccgc ccgaggcggt cgcgacggac gcggaacccg
      361 ccgcagcctc atcttccgat ccagtccatt cgtcctatcc aacatccctt accgctactt
      421 gcgtaggagc agaacgatga agaaggctat ccagcgcgcc tttgccgtgt ccctgctggc
      481 cggtgccgtc gcctttggcg tcggcgcgca cgcggccgac gtgatcaaga tcggtgttgc
      541 cggcccgatg accggcggca acgccgcgtt cggcgagcag ttctggcgcg gggcggtgca
      601 ggccgccgag gacatcaacg ccgccggcgg cgtcaacggc aagaagatcg agctcgtgaa
      661 ggccgacgac gcctgcgagc ccaagcaggc ggtcgcggtc gccaaccgcc tcgtcgacca
      721 ggacaaggtc gatgcgatca tcggtcactt ctgttcctcc agcacgatcc cggcctccga
      781 gatctacgcc gaagccggca agctgcagat cacgccggcc tcgaccaacc cgaaggtcac
      841 cgagcgcaac atcccgacga tcttccgcac ctgcggccgt gacgaccagc agggcgccgt
      901 cgccgccgac ttcatcctca gcacgctgaa ggcgaagaag atcgcggtga tccacgacaa
      961 ggacacctac ggccagggcc tcgccgatgc gatgaaggcc tcggccgcca agaagggcgc
     1021 caaggaagtc ctctacgagg gcctgacccg cggcgagaag gacttcaacg cgctcgccac
     1081 caagatcaag gccgccgatg ccgatgccgt gtacttcggc ggtctcgccg ccgaggccgg
     1141 tccgctggtg cgccagctgc gcgaacaggg ctcgaccgcg gccttcatct ccggcgacgg
     1201 catcgtcgac accgccttcg tcaccgccgc cggcggcccg cagttctcga agggcgtgta
     1261 catgaccttc ggtgccgacc cgcgcaacct cccgggcggc aagtcgctgg tcgagaagtt
     1321 ccgcaaggcc ggctacgagc ccgagggcta cacgctctac agctacgcga ccctgcaggc
     1381 ggtcaccgag gcgatgaagg ccaccaagtc caccgacggc gccacgctcg cgaagtggct
     1441 gaagggccac accgtcaaca ccgtcatggg cccgaaggcc tgggacgcca agggtgacct
     1501 gaaggtctcc gactacgtca tgtaccagtg ggacgacaag ggcggctacg ccgaactgaa
     1561 gaagtaagcc cgcgtccgca acgagactgc gacccccgcc acccgatccg gggcgggggt
     1621 cgacttttgt cggggctgcg cgcgccgtga tccgtgcgaa gccgccgccc gcgaccttga
     1681 ccggggagat ttccggatgg actggcacat cctcgcccag cagctcgtca acgggctgac
     1741 gctgggctcg atctacggcc tgatcgccat cggttacacg atggtgtacg gcatcatcgg
     1801 catgatcaat ttcgcgcacg gcgacgtgta catggtctcg gcctacctga ccgcgatcgc
     1861 gatcgccgtg ctcaccggct tcggcatcga ctcggcgccg ttcgccatcg tcatcacgct
     1921 ggcgctgacg atgttcatca ccggcgtcta cggctggtcg atcgagcgcg tcgcctaccg
     1981 gccgctgcgc ggctcgacgc ggctcgcgcc gctgatctcg gcaatcggca tctcgctggt
     2041 gctgcagaac tatgtgcagc tctcccaggg cgccaagccc cagggcgtgc cgacgctggt
     2101 cagcggcacc gtgcgcctcg gcaccgaaac caatttcgtg cagatgacgt acatccagat
     2161 cctgatcgtc gtcgtcagct tcgcggcgat ggcggcgctg acctggatca tccgcagcac
     2221 ctcgctcggc cgccagtgcc gcgccacgca gcaggaccgc aagatggcgg cgatcctcgg
     2281 catcgacacc gaccgcatca tctccaccgt gttcgtgatc ggcgcggcga tggcggcgat
     2341 cgccggcgtg ctggtgacgc tgaactacgg ctccttcgac ttcttcatcg gcttcgtcgc
     2401 cggcatcaag gcgttcacgg ccgcggtgct cggcggcatc ggctcgctgc ccggcgccat
     2461 gctcggcggc atcctgctcg gcctgttcga atcgctgttc tcgggcttcg tcaacaccga
     2521 ttacaaggac gtgttctcgt tcgcgctgct ggtgctggtg ctgatcttcc gcccgagcgg
     2581 gctgctcggt cgtcccgaag tggaaaaggt gtgagctgat gctgcccgct gcccccctcc
     2641 attcccgtat cgatgtactg gcgagcctgc gcgaggcgct gctcgccttc gtgctcgcgc
     2701 tcgtggtgtt cggcccggtc tccggcctga tgctgagcgg ctacaaggtc gaaccgcact
     2761 ggctgcggcc gctgctgctc gccgcggtcg tcgcggccgg gcgcttcgcg atccacctgc
     2821 tcggccagac cccggccggc cggcgcatgg tcgcgcgcaa tcaggcgcgc gtgcacggcg
     2881 gcgtcacggt cgccgccctc ggccagagcc cgaaccgcgc ccgcctgctg ctggtgttcg
     2941 tgctgctcgg cgccttcgtg attcccttcc tgccctggct gccggtgccg gatgcgctcg
     3001 ccgcgctcgg cggcaagtac tggctgtcga acctgatcct ggcgctgatc tacgtgctgc
     3061 tcggcctcgg cctcaacatc gtcgtcggtc tcgccggcct gctcgatctc ggctacgtcg
     3121 ccttctacgc ggtcggggcc tacttcctcg cgctcggtgc ccagtacctc ggcatcggct
     3181 tctggacggc gctcgtgtgc gcgccgctgc tcgccgcctg ctgcggcgcg gtgctcggct
     3241 tcccggtgct gcgcatgcac ggcgactacc tcgccatcgt cacgctcggc ttcggcgaga
     3301 tcatccggct ggtgctgaac aactggctgg agttcaccgg cggtccgaac ggcgcgccgg
     3361 tgccgccgcc gacgctgttc ggactcgaat tcacgcgcac ggcgcgccag ggcggcacgc
     3421 cgatccacga gttcctcggc atcgaataca acaccaacct gaagctggtg ttcacgtatc
     3481 tggtgctgct cgtggtcgtc atcctcatgg tgcggctgct gacgcgcctg cggcagattc
     3541 cgatgggccg ggcctgggag gcgctgcgcg aggacgagat cgcctgccgc tcgctcggca
     3601 tcaaccacgt cgtcgtcaag ctcaccgcct tcatgatggg tgcgatgacc ggcggcctcg
     3661 ccggcgtgtt cttcgcgacc tatcagggct tcgtgaaccc gacctcgttc acgttcttcg
     3721 aatcggcgct gatcctcgcc atcgtcgtgc tcggcgggct cggctcgacg gtcggcgtca
     3781 tcatcgccgc gctggtgctg acgctgctgc cggagctgct gcgcgagttc gccgacttcc
     3841 gcgtgctcgc cttcggcctg ctgatggtgc tgatgatgat ctggcgtccg cgcggcctga
     3901 tccgcccgca gcgtccggcc ttcgcgcccc cgcaggaggt ggcgcgatga agccgtccag
     3961 cctcctcgaa gtccgcggcc tgaccatgcg cttcggcggc atcgtcgcca acaacgacgt
     4021 gtccttcgat gtcgccgatg gctcgatcac cgcgctgatc ggcccgaacg gcgccggcaa
     4081 gaccaccgtc ttcaactgcc tgaccggctt ctaccgcgcc agcggcggca ccatccacct
     4141 cgccaccgcc gccggcgcgc cggtcgaggt cggcagcctg ctcgacaagc ccttcatcgg
     4201 cggttcgtat ctggtcgcgc gcgccggcat cgcccgcacc ttccagaaca tccggctgtt
     4261 ccgcgagatg agcgtggtcg agaacctgct ggtcgcgcag caccagcggc tcgaccgcaa
     4321 cgtgctcgcc ggcgcgctcg gcacgcggaa gttccgccag accgaacgcg acggcctcgc
     4381 ccgcgccttc cactggctgg aggtcatggg cctgaccgcc gatgccaacc gcctcgccgg
     4441 cgagctgccc tacggccgcc agcgccggct ggagatcgcg cgcgccatgt gcaccggccc
     4501 gcgtctggtc tgcctcgacg aaccggccgc cggcctcaat ccgcgcgaga cgcacgagct
     4561 gtccgaactg atccgcaccc tgcgcagcga gcaccgcgtg acggtgctgc tgatcgagca
     4621 cgacatgagt ctggtcatgg gcatttccga gcacatcgtc gtgctcgatc acggcgaggt
     4681 catcgccgcc ggcaacccgg aaaccatccg cagctccccg gccgtgatcg cggcctatct
     4741 cggcgccgag gaagccgagg tcgagggcgg ggagggcacg cgatgagtca ccagacccgg
     4801 acccctgcgg atgcgatccc gcagccgctg ctcgacgtgc gcggcatcga caccttctac
     4861 ggcgccatcc aggcgctgcg cggcgtgtcg ctgcacgtca gccgcggcga gatcgtcacg
     4921 ctgatcggtg ccaacggtgc cggcaagacc acgctgctcg gctcgatctt cggcaacccg
     4981 cgcgcgagcg tcggcagcgt gtccttcgac ggcgaggaca tcacgacgct ggcgacccac
     5041 agaatcgccc cgcgcggcat cgcgctggtg cccgagggcc ggcacatctt cccggcgatg
     5101 acggtcgagg aaaacctcgt catgggcacg atcccggtcg gcgaggccca cctcgacgag
     5161 gatcgcgcgc agatgttcga gctgttcccg cgcctgcagg aacgccgcaa ccagcgcgcc
     5221 ggcacgctct cgggcggcga gcagcagatg ctcgcgatcg cgcgcgcgct gatgagccgc
     5281 ccgcagctga tcctgctcga cgaaccctcg ctcggcctcg cgccgctggt ggtcaggcgc
     5341 atcttcgagg tgctgcgcga gatcgcgagc ctcggcaaga ccatcttcct cgtcgagcag
     5401 aacgccaacc acgcgctgaa actcgccgat cgcgcctacg tgctggtcaa cggcgagatc
     5461 cgcctgcagg gcagcggccg cgaactgctg gccgatcccg aggtgcgcaa ggcctatctg
     5521 ggcgggcact gacgccggcc ggcccgccgc agtgatcgcg gcgggccggc tggcttgcgg
     5581 ttgcgcgagg gctgcccgaa cagcggtggc ctgagcggga acgcgcacgt gtcgatcgcc
     5641 ccggcccggc caccccggcc gcagccgtca gtcgcgcatc gggtaaatcc cctgcaccgc
     5701 gatgcagaaa tgcaggttgg gcgccggttc cttgtcccgc aggtcgggca gggcgaaggt
     5761 cctcatgcca tcgccgccga actggttacc gagcagcgag tacagcgcct cgttctgcgc
     5821 gatcgacaat agctgcccct cgcagcggac ccagccgctg ggggcgtagg tatacggtaa
     5881 caacaggatt tgtccgagaa acgcttccat cgagactcct tgagcgggct gagtgcaggt
     5941 gccggcgcgg ggtgcgatcc gtgcgtacac ggatgcgcag ccacgctgcc aaccacggtc
     6001 cgtggtattg gccgcgcgca gtctaggtgc cgatgcgctg cgtcaaggta agccggggtt
     6061 gcaccggctg ggcgcgcagg gcgggtcggc atgtgcaggg cgggtcaggc gtgcatggca
     6121 tacctcgaat cacctcaccg gctttccgcg cgccgcaacc cgccttccgc cttgctccgg
     6181 agtcccgttt ccgccccgga ttgggtctat gccgccccag aatgagtcgg cgtcgacact
     6241 tcaggtatcg acgccg
//
LOCUS       DAOQJH010000044         6250 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_7056_length_6250_cov_4.787248, whole genome shotgun sequence.
ACCESSION   DAOQJH010000044 DAOQJH010000000
VERSION     DAOQJH010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 6250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6250
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7056_length_6250_cov_4.787248"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(40..918)
                     /gene="folD"
                     /locus_tag="PLQ18_01860"
     CDS             complement(40..918)
                     /gene="folD"
                     /locus_tag="PLQ18_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HNE00424.1"
                     /translation="MADIIDGKAHAAGLRAHLARRVAQLHADAGFVPGLAVVLVGEDP
                     ASQVYVRNKAAQTRECGMQSIEHRLPASTTQDELLALVATLNADPEVDGILVQLPVPA
                     HIDPERVLLAIDPAKDVDGFHPLNAGLLATGGSGLVPCTPLGSLMLLKDRLGSLAGLH
                     AVVVGRSNIVGKPMAQLLLREDCTVSVVHSRTREPAALCRQADIVVAAVGRPRMIGPD
                     WVKPGATVIDVGINRIALDDGRTKLVGDVDFDAVAALAGAITPVPGGVGPMTIACLLH
                     NTLTAACRRRGVELPD"
     gene            1115..1978
                     /gene="purU"
                     /locus_tag="PLQ18_01865"
     CDS             1115..1978
                     /gene="purU"
                     /locus_tag="PLQ18_01865"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004522850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HNE00425.1"
                     /translation="MPYAAAHFILTLACPDTTGIVAAVSGFLAEYGNNIVQSDQFGDA
                     DTGRFFLRVSFCASERLPDATAMRAAFTPIGERFAMDWEIHDTAIKPRMVILVSKFGH
                     CLNDLLYRYRVGALNVEIPAVISNHREFYQLAASYNIPFHYLPIMNDDKAAQESRILE
                     IIRHEQADLVVLARYMQVLSDAMCRELEGRAINIHHSFLPSFKGARPYHQAYERGVKR
                     IGATAHYVTADLDEGPIIEQEVAPVNHAMTPDDLVAVGRDLENVVLARAVKAHLERRV
                     LLNGRRTVVFP"
     gene            2001..2705
                     /locus_tag="PLQ18_01870"
     CDS             2001..2705
                     /locus_tag="PLQ18_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009540982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNE00426.1"
                     /translation="MNELVAAFWLAGDLLAGGDAQLYGIVVLSLQLSAGAVLVSTLLG
                     LPFGALLGLGRFPGRRVLIVITNAFMGMPPVVVGLFVYLMLSRSGPLGELGWLFRPEA
                     MVVAQVVLITPIVVALSRQIVEDLWAQLGDQLRSLGCSPLRAVPTLLREGRWSLLTVV
                     LAGFGRSVSEVGAIIIVGGNIAHVTRTMTTAIALETSKGDLPLALALGLILILIALLV
                     NVAADVVKTRAQRLEG"
     gene            2683..3423
                     /locus_tag="PLQ18_01875"
     CDS             2683..3423
                     /locus_tag="PLQ18_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007204108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNE00427.1"
                     /translation="MPSDWKAEPAGLLPLGFEAACYAVGKRTLLGPIDCVLDSTPGVH
                     LVLGPNGAGKSLFLRLAHGLLQPTGGQVRWATEAAACRQRQAMVFQHPVVLRRPVRAN
                     VEYALGLRGLARAERHRRSEEALARAGLTALAARMAPVLSGGERQRLALARAWALHPD
                     VLFLDEPTASLDPAAAAQVEALIGDFRARGTHIVMSTHDLAQARRLGGEVLFFHRGRL
                     LERAPAAGFFAQPATREAAAYVRGELFW"
     gene            complement(3528..4787)
                     /gene="rhlB"
                     /locus_tag="PLQ18_01880"
     CDS             complement(3528..4787)
                     /gene="rhlB"
                     /locus_tag="PLQ18_01880"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018719281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HNE00428.1"
                     /translation="MEKSHLTSTPFSALPLTDCLLEALEDAGFTYCTPIQDATLPLAL
                     AGHDIAGQAQTGTGKTAAFLLSTLHYLMTTPVEDGLSGPWAIMLAPTRELALQIHKDA
                     ESLGRYTGLKFAAVYGGTGYDSQRRQLEDGVDVVIGTPGRIIDFYKQGVLKLKHIEVV
                     VLDEADRMFDLGFISDIRYLLRRMPSADQRINLLFSATLSTRVMELAYEHMNNPQQIK
                     IESEQVTADKVRQTLFHVSKEDKIPLLVGLLHREKPNRAIVFVNTKRAAEQVEGWLAG
                     NSWQCAVLSGDIPQNKRERLLAQFQAGELPVLVATDVAARGLHIPDVSHVFNFDLPQD
                     AEDYVHRIGRTARIGASGDAISFACEEYVYSLPDIEDYISQKIPVEPIEPGLLAEVRP
                     ARRSEHHGERRPHGRPPRRRHNGGRPG"
     gene            4919..5245
                     /gene="trxA"
                     /locus_tag="PLQ18_01885"
     CDS             4919..5245
                     /gene="trxA"
                     /locus_tag="PLQ18_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009615069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HNE00429.1"
                     /translation="MSDQIVSVSDSSFESDVLKSELPVLVDYWAEWCGPCRMIAPILD
                     KVAEDYKGRLTVAKLNIDENQVTPTRYGVRGIPTLMIFRGGEIIGTKVGALSASQLSK
                     FIEDTI"
     gene            5496..>6250
                     /gene="rho"
                     /locus_tag="PLQ18_01890"
     CDS             5496..>6250
                     /gene="rho"
                     /locus_tag="PLQ18_01890"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HNE00430.1"
                     /translation="MNLTELKRKPASEIIEIAQELGLEGMARSRKQDVIFAILKAHAK
                     SGEDIWGDGVLEILSDGFGFLRGGDSSYLAGPDDIYVSPSQIRRFNLRTGDTVSGKIR
                     PPKEGERYFALLKVNEINYEPPENSKSKVLFENLTPLHAHRRLKLERGNGSTEDITAR
                     IIDLIAPIGKGQRGLIVSPPKAGKTVLMQNVAQSISVNHPECYLIVLLIDERPEEVTE
                     MQRTVRGEVIASTFDEPAARHVQVAEMVIEKAKR"
BASE COUNT          905 a         2114 c         2220 g         1011 t
ORIGIN      
        1 cggcctgagc gctgcgcggc gcagcgcccg ggcaggctgc tagtccggca gctcgacccc
       61 gcgccgccgg caggcggcgg tcagcgtgtt gtgcagcagg caggcgatgg tcatcgggcc
      121 gacgccgccc ggcaccggtg tgatcgcgcc ggcgagcgct gcgacggcgt cgaagtcgac
      181 atcgccgacc agcttcgtcc tgccgtcgtc gagcgcgatg cggttgatgc cgacatcgat
      241 gacggtggcg ccgggcttca cccagtccgg gccgatcatg cgcgggcggc cgaccgcggc
      301 gacgacgatg tcggcctgcc ggcagagtgc cgccggctcg cgcgtgcgcg aatgcacgac
      361 gctgaccgtg cagtcctcgc gcagcagcag ctgcgccatc ggcttgccga cgatgttcga
      421 gcgcccgacc acgaccgcgt gcaggccggc gaggctgccg aggcgatcct tcagcagcat
      481 cagcgagccg agcggcgtgc acggcaccag gccgctgccg ccggtggcga gcaggccggc
      541 gttgagcggg tggaagccgt cgacatcctt ggccgggtcg atggcgagca gcacgcgctc
      601 cgggtcgata tgcgccggca ccggcagctg cacgaggatg ccgtcgactt cggggtcggc
      661 gttcagtgtc gcgaccagcg ccagcagttc gtcctgcgtg gtgctcgccg gcaggcggtg
      721 ttcgatggac tgcatgccgc attcgcgcgt ctgcgcggcc ttgttgcgca cgtagacctg
      781 gctcgccggg tcctcgccga ccagcacgac ggcgaggccg ggcacgaagc cggcatcggc
      841 gtgcagctgc gcgacacggc gggcgagatg ggcgcgcagg ccggcggcgt gggccttgcc
      901 gtcgatgatg tcggccatgg gcgggcgtct cctggggctg atccgggggc ggggagcgga
      961 aggcgggccg catcatagcg ctgcgcagtg cttgttcgtg cagggatcgc tgcttatgct
     1021 cgcgcgttta cgtcgcgcgg gtggcttccg gtccgtgcgt ccagcccatc cgccccacga
     1081 gccggcgcgc cgcggcgccc ggacagatgc cgccatgccg tacgccgccg cccatttcat
     1141 cctgacgctc gcctgcccgg acaccaccgg catcgtcgcc gccgtgtcgg gtttcctcgc
     1201 cgaatacggc aacaacatcg tgcagagcga ccagttcggc gacgccgaca ccggccgctt
     1261 cttcctgcgc gtgagcttct gcgccagcga gcgcctgccg gatgccacgg cgatgcgcgc
     1321 ggccttcacg ccgatcggcg agcgcttcgc gatggactgg gagatccacg acaccgcgat
     1381 caagccgcgc atggtcatcc tggtgtcgaa gttcggccac tgcctgaacg acctgctgta
     1441 ccgctaccgg gtcggggcgc tgaacgtcga gattccggcc gtgatctcga accaccgcga
     1501 gttctaccag ctcgccgcca gctacaacat tccgttccac tacctgccga tcatgaacga
     1561 cgacaaggcg gcgcaggagt cgcgcatcct ggagatcatc cgccacgagc aggccgatct
     1621 ggtcgtgctc gcgcgctaca tgcaggtgct gtcggacgcc atgtgccgcg agctcgaagg
     1681 ccgtgcgatc aacatccacc acagcttcct gccgagcttc aagggcgcgc ggccctacca
     1741 tcaggcctac gagcgcggcg tcaagcgcat cggcgcgacc gcgcactacg tgacggccga
     1801 cctcgacgaa ggcccgatca tcgagcagga ggtcgcgccg gtgaaccacg cgatgacgcc
     1861 ggacgatctg gtcgcggtcg ggcgcgatct cgagaacgtc gtgctcgcac gcgcggtcaa
     1921 ggcccacctc gaacgccgcg tgctgctcaa cggccggcgc acggtcgtgt tcccgtaagg
     1981 tccggcgggt ggcatcgctc gtgaacgagc tcgtcgccgc attctggctg gccggtgacc
     2041 tgctcgccgg cggcgatgcg cagctctacg gcatcgtcgt gctgtccttg cagctcagtg
     2101 ccggcgcggt gctggtctcg acgctgctcg gcctgccgtt cggggcgctg ctcgggctcg
     2161 ggcgctttcc gggccggcgc gtgctgatcg tcatcaccaa cgccttcatg ggcatgccgc
     2221 cggtggtcgt cggcctgttc gtctatctga tgctgtcgcg cagcggcccg ctcggcgaac
     2281 tcggctggct gttccggccc gaggcgatgg tcgtcgcgca ggtggtgctg atcacgccga
     2341 tcgtcgtcgc gctgagccgc cagatcgtcg aggacctctg ggcgcagctc ggcgatcagc
     2401 tgcgctcgct cggctgcagc ccgctgcgcg cggtgccgac gctgctgcgg gaggggcgct
     2461 ggagcctgct gaccgtcgtg ctcgccggtt tcgggcgctc ggtgtccgag gtcggcgcga
     2521 tcatcatcgt cggcggcaac atcgcgcacg tgacgcgcac gatgaccacg gcgatcgcgc
     2581 tggagaccag caagggcgac ctgccgctgg cgctggcgct cgggctgatc ctgatcctga
     2641 tcgcgctgct cgtgaacgtg gctgccgatg tcgtgaagac ccgtgcccag cgactggaag
     2701 gctgagccgg ccggcctgct gccgctcggc ttcgaggcgg cgtgctacgc ggtcggcaag
     2761 cgcacgctgc tcgggccgat cgactgtgtg ctcgactcaa cacccggcgt gcatctggtg
     2821 ctcggcccca acggcgccgg caagagcctg ttcctgcgcc tcgcgcacgg cctgctgcag
     2881 ccgacgggcg ggcaggtgcg ctgggcgacg gaggccgcgg cctgccggca gcgtcaggcg
     2941 atggtgttcc agcacccggt cgtgctgcgc cgtccggtgc gcgccaacgt cgaatacgcg
     3001 ctcggcctgc gcggcctggc gagggccgaa cgccatcggc gcagcgagga ggccctggcg
     3061 cgtgccggcc tgacggcgct ggccgcgcgc atggcgccgg tgctctccgg cggcgagcgc
     3121 cagcggctgg cgctggcgcg ggcctgggcg ctgcacccgg acgtgctgtt cctcgacgag
     3181 ccgaccgcga gcctcgatcc ggccgctgcc gcgcaggtcg aggcgctgat cggggatttc
     3241 cgcgcgcgcg gcacgcacat cgtcatgagc acgcacgatc tggcgcaggc gcgccggctc
     3301 ggtggcgagg tgctgttctt ccaccgcggc cggctgctgg aacgcgcgcc ggccgccggt
     3361 ttcttcgcgc agccggccac gcgcgaggcg gcagcctacg tgcgtggcga gctgttctgg
     3421 tagttcatgc cgatcaaggc tgaactcccg gtcttttcag cccctgcccc cttgcggggg
     3481 aggggcgtgg agagggagat cagcaacgca ttcacgcgtg atcggtatca gcccgggcgg
     3541 ccgccgttgt gccggcggcg cggcggacgg ccgtgcggac ggcgctcgcc gtggtgctcg
     3601 ctgcgcctgg ccgggcgcac ctcggcgagc aggccgggct cgatcggctc gaccggaatc
     3661 ttctggctga tgtagtcctc gatgtccggc agcgagtaga cgtactcctc gcaggcgaag
     3721 ctgatcgcgt cgcccgaggc gccgatgcgc gccgtgcggc cgatgcggtg cacgtagtcc
     3781 tcggcgtcct gcggcaggtc gaagttgaag acgtggctca cgtccgggat gtgcaggccg
     3841 cgtgcggcga catcggtcgc caccagcacc ggcagctcgc cggcctggaa ctgcgcgagc
     3901 aggcgctcgc gcttgttctg cgggatgtcg ccggagagca ccgcgcactg ccagctgttg
     3961 ccggccagcc agccctcgac ctgctcggca gcgcgcttgg tgttgacgaa cacgatggcg
     4021 cggttcggct tctcgcggtg cagcaggccg accagcagcg ggatcttgtc ttccttcgag
     4081 acgtggaaca gcgtctggcg caccttgtcg gcggtgacct gttcggactc gatcttgatc
     4141 tgctgcgggt tgttcatgtg ctcgtaggcg agctccatga cccgggtcga cagcgtcgcc
     4201 gagaacagca ggttgatgcg ctggtcggcc gagggcatcc ggcgcagcag gtagcggatg
     4261 tcggagatga agccgaggtc gaacatgcgg tcggcctcgt cgagcacgac gacttcgatg
     4321 tgcttgagct tcagcacgcc ctgcttgtag aagtcgatga tccggcccgg cgtgccgatg
     4381 acgacatcga cgccgtcttc cagctggcgg cgctgcgagt catagccggt gccgccgtag
     4441 acggcggcga acttcaggcc ggtgtagcgg ccgagggatt cggcgtcctt gtggatctgc
     4501 agtgcgagtt cgcgggtggg ggcgagcatg atcgcccagg ggccggagag tccgtcctcg
     4561 accggcgtcg tcatcaggta gtgcagcgtg gacagcagga aggcggccgt cttgccggtg
     4621 ccggtctggg cctggccggc aatgtcgtga ccggcgagcg cgagcggcag cgtggcgtcc
     4681 tggatcgggg tgcagtacgt gaatccggcg tcttcgaggg cttccagcag gcagtcggtc
     4741 aggggcaacg ccgagaacgg cgtggaggtc agatggcttt tttccatggg gagcgcaggg
     4801 gataccgcgg gagggcggcc ggtgccgaca cttgccagcg ttccggcgtt tcggctgaaa
     4861 tgagtctttg gtttgctaag gcgaccgtgt gagcggcgcc attctggagg cgaatgccgt
     4921 gagtgatcaa atcgtttctg tgtccgacag cagcttcgaa agcgatgtcc tgaagtcgga
     4981 actgccggtg ctggtcgact actgggccga atggtgcggt ccgtgccgca tgatcgcccc
     5041 gatcctcgac aaggttgccg aggactacaa gggccggctg accgtcgcca agctcaacat
     5101 cgacgagaat caggtgacgc cgacccggta cggcgtgcgc ggcatcccga cgctgatgat
     5161 cttccgcggc ggcgagatca tcggcaccaa ggtgggcgcg ctgtcggcct cgcagctgag
     5221 caagttcatc gaggacacca tctgaaaccc gccgccgggc cgcgccgcgc ccggccgttt
     5281 cgcccgaatc cggccggcgt ccgacttctg gacgccggcc gtgtcgcgtg ttagcttccg
     5341 ccatcatttt ttctgcgggc gcccgtctcc ggtgcttccc ccgcaccctc gacgtccccc
     5401 gtcgaccccg ccagcccccg caccgcgcat ccaggccctt ctccgcaccc cgggccggtg
     5461 ccattccgca gcccccgcgc cggaagcacg cctacatgaa tctgactgaa ctcaagagaa
     5521 aacccgcgtc cgagatcatc gagatcgcgc aggagctggg cctcgaaggc atggcgcgtt
     5581 cgcgcaagca ggacgtgatc ttcgcgatcc tgaaggcgca cgcgaagagc ggggaggaca
     5641 tctggggcga cggcgtgctc gaaatcctct ccgacggttt cggcttcctg cgcggcggag
     5701 acagttcgta tctggccgga cccgacgaca tctacgtgtc gccgtcgcag atccggcgct
     5761 tcaacctgcg caccggcgat accgtcagcg gcaagatccg cccgcccaag gaaggcgagc
     5821 gctacttcgc gctgctgaag gtcaacgaga tcaactacga accgcccgag aactccaaga
     5881 gcaaggtgct gttcgagaac ctgacgccgc tgcacgcgca ccggcgcctg aagctcgaac
     5941 gcggcaacgg ttcgaccgag gacatcacgg cgcggatcat cgacctgatc gcgccgatcg
     6001 gcaaaggcca gcgcggcctg atcgtctcgc cgccgaaggc cggcaagacc gtgctgatgc
     6061 agaacgtcgc gcagtcgatc tccgtgaacc atcccgagtg ctacctgatc gtgctgctga
     6121 tcgacgagcg gcccgaggaa gtcaccgaga tgcagcgcac cgtgcgcggc gaggtcatcg
     6181 cctcgacctt cgatgagccc gccgcccgcc acgtgcaggt cgccgagatg gtcatcgaga
     6241 aggccaagcg
//
LOCUS       DAOQJH010000045         6234 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_7086_length_6234_cov_4.208772, whole genome shotgun sequence.
ACCESSION   DAOQJH010000045 DAOQJH010000000
VERSION     DAOQJH010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 6234)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6234)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6234
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7086_length_6234_cov_4.208772"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..513
                     /locus_tag="PLQ18_01895"
     CDS             <1..513
                     /locus_tag="PLQ18_01895"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNE00431.1"
                     /translation="MAINVSSRQFHDYRFVDRVQTILQNHELPGDAIVLELTERLFLG
                     DDGTSIEVMRELAEQGVRIAIDDFGTGYSSLSYLSRFPVHLVKIDRAFVKGLPEDSES
                     AGLSAAIVAMAGALGLEVICEGVETPEQQTYLAGLGAQHGQGWLFGKPLPSADIRQLL
                     LNRASSAPAA"
     gene            complement(517..1260)
                     /gene="cysZ"
                     /locus_tag="PLQ18_01900"
     CDS             complement(517..1260)
                     /gene="cysZ"
                     /locus_tag="PLQ18_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HNE00432.1"
                     /translation="MISPFLRGARCLLTGLSLLGHERLRSFVLLPLVINLLLFGGGLW
                     WGGSELSAWLDAQIAKLPDWLHWLHWLLVPLLVAAAALVTFYGFSIVANLVGAPFNGW
                     LAERAEALFDPAAQVPERPLWQEVTIAFASALRATLYFLLRAIPLLLLFFIPVVNIAA
                     PFIWAAFGAWMLALQYLDYPLGNHGIDFSEQRRAHRADRARTFGFGCAVLLLTLVPVL
                     NFVAMPAAVIGATALAVERRRTAGVAALR"
     gene            1527..2243
                     /gene="tolQ"
                     /locus_tag="PLQ18_01905"
     CDS             1527..2243
                     /gene="tolQ"
                     /locus_tag="PLQ18_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002812522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HNE00433.1"
                     /translation="MTAEMSFTHLILQASWVVQLVMLLLLLISLASWTVIFRKRIALS
                     HARRQADTFEDDFWSGVDLAALYTRVSRREDEAEGLEAVFLAGFQEFLRLRAQTGVDP
                     ESVVAGSQRAMRAALSRETDELELQLPVLATAASVSPYIGLFGTVWGIMNAFMGLGAA
                     KTATLQQVAPGIAEALIATAIGLFAAIPAVIAYNRYSTEVDRLVNRYDTFAEEFSNIL
                     QRQAHSLRPRQPQAEVHGPA"
     gene            2230..2658
                     /gene="tolR"
                     /locus_tag="PLQ18_01910"
     CDS             2230..2658
                     /gene="tolR"
                     /locus_tag="PLQ18_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006475520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolR"
                     /protein_id="HNE00434.1"
                     /translation="MARRSSRKKSVSEINVVPLIDVMLVLLIIFMVTAPLLNQGVEVD
                     LPKGVAEALPQQEQEPMVLTVDREGHLYLNRADDPKLALSTEDLLIRARAVLQREPKT
                     VVLVKGDKGVEYGKVVAAMHLLTQAGAAKVGLSMDLPDGA"
     gene            2666..4057
                     /gene="tolA"
                     /locus_tag="PLQ18_01915"
     CDS             2666..4057
                     /gene="tolA"
                     /locus_tag="PLQ18_01915"
                     /inference="COORDINATES: protein motif:HMM:TIGR02794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HNE00435.1"
                     /translation="MGPDLVSPKPHHRSPRQAGRLRSLVLSVALHGSLLALALLTLSP
                     HVMPPPPAAPKPPESGEIIQATVVDEAKVAAEVQRLEAAERQKREAEAAHQRQLDQQA
                     RAAEERRAREEQRLKDLQARQEEAARRAEAEQRRQEEATRRAEAEQRRQEDAARRAEA
                     ETKQRLAQLAQQQQEAERKAQEARAKADADARAARVAEERREAEEKRAADAKAKAEAE
                     ARARAEAARKAEAEKKKLAAEAEAREKAEDEAREKAEAAKKAAAAARAKADADAKAKA
                     DADAKARAARAEADAKAKADADAKAKAARAEADAKAKADADAKAKADAKAKAEAEAKA
                     RADAEAKAAAARKDLADLLAREAKERAAQQQVTAAALSAYQSLLVNHVEPRYQPPPAC
                     PEGGVADISIKLSPGGEVVSASVRRSSGNAVCDGAALAALKRASPLPLPRDPVLAERL
                     QQESFVFKFKVAE"
     gene            4069..5361
                     /gene="tolB"
                     /locus_tag="PLQ18_01920"
     CDS             4069..5361
                     /gene="tolB"
                     /locus_tag="PLQ18_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005418511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HNE00436.1"
                     /translation="MLKTLPTRARAFAALLLTLAAVTTARAELTIEIVKGVEGALPIA
                     IAPFGGNAGLPADIAAIITADLKRSGRFSPLASSQYADRPTELSQVNYASFRSIGVPH
                     LAVGQVRAGGAGYAVQFQLADVYQGSQLLGSSFDVRSGDLRRVAHHIADLIYEKLTGE
                     KGAFNTRIAYVTARGTSSYTLVVADSDGYNPQVVLRSPLPVMSPAWSPDGSKLAYVSF
                     ENRAAQIVVQNVFTGARQTISASPGINGAPAWSPDGSQLAFTLSKDGNPEIYRYDLAS
                     RQTQRLTSTSAIDTEATWSPDGGSIVFTSDRGGGPQLYRMPAGGGGAERLTFSGDYNS
                     RPTFSPDGKSIAYVTRSGGRFHIAVMDASGRNTRLLTDGGMDESPSFAPNGGMILYAT
                     QQGGRGVLAAVSTDGRVRQTLLSTDGEVREPAWSPYGR"
     gene            5340..5999
                     /gene="pal"
                     /locus_tag="PLQ18_01925"
     CDS             5340..5999
                     /gene="pal"
                     /locus_tag="PLQ18_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016854894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HNE00437.1"
                     /translation="MVAVRPLNRSPPIRPDPEEPAQESDPNMNAERIPTPKNRSLQTL
                     KTVLAAATLATLAACSSTGSTSGDGSSGSGGSGTGAYGASAGGQYGSGGAYGSGGPAG
                     GYVGGPGATQEQRVVYFDYDSADIRADSRPIIQAHAQYLQQTPGSVAILEGHADERGS
                     REYNIALGERRSVAVRRAMEALGVQPNQMRTVSYGEERPAVGGHDESAYAQNRRVEIA
                     Y"
     gene            6014..>6234
                     /locus_tag="PLQ18_01930"
     CDS             6014..>6234
                     /locus_tag="PLQ18_01930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE00438.1"
                     /translation="MTAAALRRLALATALLAALPAGAQQRDQSSLIYELLDRINQLED
                     EVRQLRGDVEMLRYRQEQGAHAQPAPAAPP"
BASE COUNT         1056 a         2281 c         2131 g          766 t
ORIGIN      
        1 atggccatca acgtctcgtc gcgccagttc cacgactacc gcttcgtcga ccgcgtgcag
       61 acgatcctgc agaaccacga gctgccgggc gatgcgatcg tgctggagct gaccgagcgg
      121 ctgttcctcg gcgacgacgg cacctcgatc gaggtcatgc gcgaactcgc cgagcagggc
      181 gtgcgcatcg cgatcgacga cttcggcacc ggctattcgt cgctgagcta cctgagccgc
      241 ttcccggtgc atctggtcaa gatcgaccgc gccttcgtca agggcctgcc cgaagactcc
      301 gagagcgccg gactcagcgc cgcaatcgtc gcgatggccg gcgcgctcgg cctcgaagtc
      361 atctgcgaag gcgtcgagac ccccgagcag cagacctatc tcgccggcct cggcgcgcag
      421 cacggtcagg gctggctgtt cggcaagccg ctgccttcgg ccgacatccg ccagctgctg
      481 ctcaaccgcg cgtcgagcgc gccggcggcc tgagcttcag cgaagcgcgg cgacgcctgc
      541 cgtgcgccgg cgctcgaccg ccagcgccgt cgccccgatc accgctgccg gcatcgcgac
      601 gaagttcagc accggcacca gcgtcagcag cagcaccgcg cagccgaagc cgaaggtgcg
      661 ggcgcggtcg gcacggtgtg cgcggcgctg ttcgctgaag tcgatgccgt gattgccgag
      721 cgggtagtcg aggtactgca gcgccagcat ccacgcgccg aacgccgccc agatgaaagg
      781 tgcggcgatg ttgacgacag ggatgaagaa cagcagcagc agcgggatcg cgcgcagcag
      841 gaagtacagc gtcgcgcgca gcgcgctggc aaacgcaatc gtcacctcct gccacagcgg
      901 ccgttccggc acctgcgccg cgggatcgaa cagcgcctcg gcgcgctcgg ccagccagcc
      961 gttgaacggt gcgccgacca gattggcgac gatgctgaat ccatagaagg tcaccagcgc
     1021 cgccgcggcg acgagcagcg gcaccagcag ccagtgcagc cagtgcagcc agtcgggcag
     1081 cttcgcgatc tgcgcatcga gccaggccga cagctcgctg ccgccccacc acaggccgcc
     1141 gccgaacagc agcaggttga tcacgagcgg cagcagcacg aagctgcgca accgctcgtg
     1201 gccgagcagg ctcaggccgg tcagcaggca gcgcgcgccg cgcaggaagg gactgatcat
     1261 cgggattcgc tcatgcagcg ggacgggagg tgtgcggctg cgacagcggg ccgcggaact
     1321 ttacgccgcc ggcgccgttc tgccgtcgca tcgtgctgat acaatgctgc accgcattca
     1381 agcgtgccgg tagtccggtg cgccgcgccc ttccccccgt gcatcggatg cccgccccgg
     1441 aacttccccg gcgggccggc cgcattgtcc ggcgcggagc cggtcgcgcc gccgtcccga
     1501 cacgcgccgt tccggagtag gttgcgttga ccgccgagat gtccttcaca cacctgatcc
     1561 tgcaggcgag ctgggtggtg cagctggtga tgctgctgct gctgctgatc tcgctggcct
     1621 catggacggt gatcttccgc aagcgcatcg cgctcagcca cgcccgccgt caggccgaca
     1681 ccttcgagga cgacttctgg tccggcgtcg acctcgccgc gctgtacacg cgcgtcagcc
     1741 gccgcgagga cgaggccgag ggcctcgaag ccgtgttcct cgccggtttc caggagttcc
     1801 tgcgcctgcg cgcgcagacc ggcgtcgatc cggaatccgt ggtcgccggc agccagcgcg
     1861 cgatgcgcgc ggcgctgtcg cgcgagaccg acgagctcga actgcagcta ccggtgctcg
     1921 cgaccgcggc ctcggtcagc ccgtacatcg gcctgttcgg cacggtctgg ggcatcatga
     1981 acgccttcat ggggcttggt gccgccaaga ccgcgacgct gcagcaggtc gcgcccggca
     2041 tcgccgaggc gctgatcgcg accgcgatcg gcctgttcgc ggcgattccg gcggtgatcg
     2101 cgtacaaccg ctactcgacc gaggtcgatc gcctcgtcaa ccgctacgac accttcgccg
     2161 aggagttctc gaacatcctg cagcgtcagg cgcacagcct gcgcccgcgc cagccgcagg
     2221 ccgaagtcca tggcccggcg tagctcgcgc aagaaatcgg tcagcgagat caacgtcgtg
     2281 ccgctgatcg acgtgatgct ggtgctgctg atcatcttca tggtcacggc gccgctgctg
     2341 aaccagggcg tcgaggtcga cctgccgaaa ggcgtcgccg aagccctgcc gcagcaggag
     2401 caggagccga tggtgctgac cgtcgatcgc gaggggcatc tgtacctgaa ccgcgccgac
     2461 gacccgaagc tcgcgctgag caccgaggat ctgctgatcc gtgcccgtgc cgtgctgcag
     2521 cgcgagccga agaccgtcgt gctggtcaag ggcgacaagg gcgtcgaata cggcaaggtg
     2581 gtcgcagcca tgcacctgct gacccaggcc ggggccgcca aggtcggcct gtcgatggac
     2641 ctgcccgacg gtgcctgagc acgacatggg tcctgatctc gtgagcccga aaccgcacca
     2701 ccgctcgccc cgccaggccg gccggctgcg ctcgctcgtg ctgtcggtgg cgctgcacgg
     2761 ctcgctgctg gcactggcgc tgctgacgct gagcccgcat gtgatgccgc cgcccccggc
     2821 tgcgccgaag ccgccggagt cgggcgagat catccaggcc accgtcgtcg atgaggcgaa
     2881 ggtcgctgcc gaggtccagc gcctcgaagc cgccgagcgc cagaagcgcg aggccgaggc
     2941 cgcacaccag cgccagctcg accagcaggc acgcgccgcc gaggagcggc gcgcccgcga
     3001 ggaacagcgg ctgaaggatc tgcaggcccg ccaggaggaa gccgcccgtc gtgccgaggc
     3061 cgagcagcgc cggcaggagg aagccacgcg ccgggccgag gccgagcagc gccggcagga
     3121 ggatgccgcg cgtcgcgccg aagccgagac gaagcagcgc ctcgcgcagc tcgcccagca
     3181 gcagcaggag gccgaacgca aggcgcagga agcccgcgcc aaggccgacg ccgacgccag
     3241 agccgcccgc gtcgccgagg aacgccgcga ggccgaggaa aagcgtgccg ccgatgcgaa
     3301 ggccaaagcc gaagccgaag ccagagcgcg cgccgaggcc gcgagaaagg ccgaggccga
     3361 aaaaaaaaag ctagcggctg aggccgaggc ccgcgagaag gccgaagacg aggcccggga
     3421 gaaagccgaa gcggcgaaga aggcggccgc agccgccagg gcgaaggccg acgccgatgc
     3481 aaaggccaaa gccgatgccg atgcgaaggc cagggccgcc agggcggaag ccgacgccaa
     3541 ggccaaagcc gacgccgatg cgaaggccaa ggccgccagg gcggaagccg atgccaaggc
     3601 caaagccgac gccgatgcga aggccaaggc cgacgcgaag gccaaagcag aggccgaggc
     3661 caaggccagg gcggatgccg aagccaaggc cgcggcagcg cggaaggatc tcgccgacct
     3721 gctcgcacgc gaagccaagg agcgcgccgc gcagcagcag gtcacggccg cagcactcag
     3781 tgcctaccag agcctgctgg tcaaccacgt cgagccgcgc taccagccgc cgccggcctg
     3841 ccccgagggc ggggtcgcgg acatttcgat taagctgtcg ccgggcggcg aggtcgtctc
     3901 ggccagcgtg cggcgcagca gcggcaatgc cgtctgcgac ggcgccgcac tcgccgcact
     3961 gaagcgcgcc tcgccgctgc cgctgccacg cgatcccgtg ctggccgagc ggctgcagca
     4021 ggaaagcttc gtgttcaagt tcaaggttgc cgagtgagga agcgcagggt gctcaagact
     4081 ctcccgacgc gcgcgcgagc ctttgccgcg ctgttgctga cgctggccgc cgtgaccacg
     4141 gcccgtgccg aactgaccat cgagatcgtc aagggtgtcg aaggggccct gcccattgcg
     4201 attgcgccgt tcggcggcaa cgccgggctg ccggccgaca ttgccgcgat catcacggcc
     4261 gatctcaagc gcagcggccg cttctcgccg ctcgcatcga gccagtacgc cgaccgcccg
     4321 accgagctgt cgcaggtcaa ctacgcgagc ttccgctcga tcggcgtgcc gcacctcgcg
     4381 gtcggtcagg tgcgcgccgg cggggccggt tacgccgtgc agttccagct cgccgatgtc
     4441 tatcagggct cgcagctgct cggcagcagc ttcgacgtgc gcagcggcga cctgcggcgc
     4501 gtcgcgcacc acatcgccga cctgatctac gagaagctca ccggcgagaa gggcgcgttc
     4561 aacacgcgca tcgcctacgt caccgcgcgc ggcaccagca gctacacgct ggtggtcgcc
     4621 gattccgacg gctacaaccc gcaggtcgtg ctgcgctcgc cgctgccggt gatgtcgccg
     4681 gcctggtcgc cggacggcag caagctcgcc tacgtcagct tcgagaaccg cgccgcgcag
     4741 atcgtcgtgc agaacgtctt caccggcgct cgccagacca tctcggcctc gcccggcatc
     4801 aacggtgcgc cggcctggtc gccggacggc agccagctcg cgttcacgct gtcgaaggac
     4861 ggcaaccccg agatctatcg ctacgacctc gcctcgcgcc agacgcagcg cctgaccagc
     4921 acctcggcga tcgacaccga ggccacgtgg tcgccggacg gcggctcgat cgtgttcacg
     4981 tccgatcgcg gcggcggtcc gcagctctac cggatgccgg cgggcggcgg cggcgccgag
     5041 cgcctgacct tctccggcga ctacaactcg cggccgacct tctcgccgga cggcaagagc
     5101 atcgcctacg tcacgcgcag cggcggacgc ttccacatcg ccgtcatgga cgcgagcggc
     5161 cgcaacaccc gcctgctgac cgacggcggc atggacgaat caccgagctt cgcgccgaac
     5221 ggcggcatga tcctctacgc cacgcagcag ggcgggcgcg gcgtgctcgc ggccgtgtcc
     5281 accgacggcc gcgtcaggca gacgctgctg tcgaccgacg gcgaagtccg cgaacccgcg
     5341 tggtcgccgt acggccgctg aaccgcagcc ctccgatccg ccccgatccc gaagaacccg
     5401 cacaggaatc cgatccgaac atgaacgccg agcgcatccc gaccccgaag aaccgctccc
     5461 tgcagacgct gaagaccgtg ctcgccgccg ccacgctggc aacgcttgcc gcctgctcct
     5521 cgaccggcag cacctccggc gatggttcca gcggcagcgg cggcagcggc accggagcct
     5581 acggcgccag tgccggcggc cagtacggca gcggcggtgc ctacggcagc ggcggcccgg
     5641 ccggcggcta cgtcggcggc cccggcgcca cgcaggagca gcgcgtcgtg tacttcgact
     5701 acgacagcgc cgacatccgc gccgactcgc ggccgatcat ccaggcgcac gcgcagtacc
     5761 tgcagcagac gccgggctcg gtggcgatcc tcgaaggcca cgccgacgag cgcggcagcc
     5821 gcgaatacaa catcgccctc ggcgagcggc gcagcgtcgc ggtgcgtcgc gccatggaag
     5881 cgctcggcgt gcagccgaac cagatgcgca cggtcagcta cggcgaggag cgcccggccg
     5941 tcggcggcca cgacgagagc gcctacgcgc agaaccgtcg cgtcgagatc gcctactgag
     6001 ccgcgccgcc gccatgaccg ccgccgcgtt gcgccgcctc gccctcgcga ccgccctgct
     6061 cgccgcgctg cccgccggcg cccagcagcg cgaccagtcc tcgctgatct acgagctgct
     6121 cgatcgcatc aaccagctcg aagacgaagt ccgccagctg cgcggcgacg tcgagatgct
     6181 gcgctaccgc caggaacagg gcgcgcacgc ccagccggct ccggcagccc cgcc
//
LOCUS       DAOQJH010000046         6218 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_7117_length_6218_cov_4.506571, whole genome shotgun sequence.
ACCESSION   DAOQJH010000046 DAOQJH010000000
VERSION     DAOQJH010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 6218)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6218)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6218
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7117_length_6218_cov_4.506571"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1020)
                     /locus_tag="PLQ18_01935"
     CDS             complement(<1..1020)
                     /locus_tag="PLQ18_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012981424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HNE00439.1"
                     /translation="MKYKDLRDFIARLEADGQLRRIRVPVSPRLEMTEICDRVLRARG
                     PALLFERPAGHSVPVLANLFGTPERVALGMGAESVAALREVGRLLAFLKEPEPPKGLK
                     DAWNSLPVFRKVLDMAPKEISRPPCQECVLEGEAVDLARLPVQTCWPGDAGPLITWGL
                     VVTKGPHKARQNLGIYRQQVIGRNRVIMRWLSHRGGALDFREWQQAHPGRPFPVAVAL
                     GADPATILGAVTPVPDTLSEYAFAGLLRGAKTELARCLGCDLQVPASAEFVLEGHIAP
                     GDTAPEGPFGDHTGYYNEVDTFPVFTIERITHRREPIYHSTYTGRPPDEPAILGVALN
                     EVFVPI"
     gene            1286..1864
                     /locus_tag="PLQ18_01940"
     CDS             1286..1864
                     /locus_tag="PLQ18_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HNE00440.1"
                     /translation="MDFLLRLSRGIDWLNEHVGKTVYWLILIMVIICTGNALMRKLFN
                     ISSNGFLEIQWYLFAAVFLLGAAYTLQRNEHVRIDVVVGRFSLRTQIIIDILGTLLFL
                     TPLCLLMIVFGWPYFLDSWHTQEYSPNPGGLILWPVKVLIPIGFVLLQLQGLSQLIKQ
                     IGALTGHVDPHTLVKSHHGHAPADVPAPHTPN"
     gene            1880..3343
                     /locus_tag="PLQ18_01945"
     CDS             1880..3343
                     /locus_tag="PLQ18_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006466101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HNE00441.1"
                     /translation="MEAFIVANMAPLMFGALVIFLLLGYPVAFALAANGMVFGLIGIE
                     YGLLKPELLQALPQRVFGIMQNDTLLAIPFFTFMGLILERSGMAEDLLDTIGQLFGPI
                     RGGLAYAVIFVGALLAATTGVVAASVISMGLISLPIMLRYGYDKKLATGVIAASGTLA
                     QIIPPSLVLIIMADQLGRSVGDMYAGALIPGLVLTGMYVGYVFLTATFKPGAAPALPP
                     EARSLRGAALGRRVLVSLVPPLVLIFLVLGTIFIGVATPTEGGAMGASGAIILALINR
                     RLDWKLVRQALDQTAKLSCFVLFILIGSTVFGLVFRAVNGDLWVEHLLTALPGGEVGF
                     LIVVNILMFALAFFLDFFELAFILVPLLGPVAEKLGIDLIWFGVLLGVNMQTSFMHPP
                     FGFALFYLRSVAPPDIRTTHIYWGAVPFVCIQIIMVALLIAFPGLVSVEKHKVDNSAP
                     VQIEMPAVDYGAPAGDGGYAPPSYDYQPAPPPEPAKP"
     gene            complement(3455..4540)
                     /locus_tag="PLQ18_01950"
     CDS             complement(3455..4540)
                     /locus_tag="PLQ18_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017509142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HNE00442.1"
                     /translation="MERRDFLKTAGAGLAAAATVAAPAIVRAEPQIKWRLASSFPKSL
                     DTIYGAAEVLANRVREITEGKFDIRVFAGGEIVPGFQTLDAAQQGTVECGHATMYYFI
                     GKNKAFAFDTALPFGLTCRQQNAWLYHGGGLELYREFLKDYGVISFPGGNTGTQMGGW
                     FRNEIKGLADVKGLKMRIAGLGGEIMSRLGAVPQQIPGGDIYPSLEKGTIDAAEWIGP
                     YDDEKLGFVKVAPHYYFPGWWEGGPNVSFFVNQGEWDKLPKSYQAAFTAAAAEANIDM
                     MAKYDAKNPPALSRLVRDGAKLHPFPRDVMEAAFKTANELYEEEAGKNPQFKKLYDSW
                     KTFRSAEYQWFRVAEATYANFVYSQRL"
     gene            complement(4693..5118)
                     /locus_tag="PLQ18_01955"
     CDS             complement(4693..5118)
                     /locus_tag="PLQ18_01955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE00443.1"
                     /translation="MKIRILFLAAALLSGACGTPATAPSFRTSATVVLTTGLNDRQQP
                     VNAVEALSLDQERFHVFTQWNQLAPGEHTQLVRVLDGERKPVFSDAQRFTADNVSRST
                     VSSFQINRYLQQPGQWQVEVYLDNERFAQKGVKVLPAAN"
     gene            complement(5197..5967)
                     /gene="xth"
                     /locus_tag="PLQ18_01960"
     CDS             complement(5197..5967)
                     /gene="xth"
                     /locus_tag="PLQ18_01960"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008853 - exodeoxyribonuclease III
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HNE00444.1"
                     /translation="MRKIATWNVNSLKVRLPQVLDWLAAESPDVLALQETKLQDADFP
                     AGAIRAAGYEVVFAGQKTYNGVALLSREPADAVVTELPGLADPQRRVLGATVAGVRVL
                     DLYVPNGQAVGSDKYAYKLDWLAKLAAFLETERAAHPELVVLGDFNIAPEDRDVHDPE
                     AWRGQILCSGPERAAFRGLLDAGFRDAFRLFEQPPKTFSWWDYRGGGFRRNHGLRIDH
                     ILLSPALAARCRGCRIDTGPRRLERPSDHAPVLASFDT"
BASE COUNT          921 a         2153 c         2090 g         1054 t
ORIGIN      
        1 gatcggcacg aacacctcgt tcagcgccac gccgaggatc gccggctcgt cgggcgggcg
       61 gccggtgtag gtcgagtgat agatcggctc gcgccggtgc gtgatgcgct cgatcgtgaa
      121 caccggaaag gtgtcgacct cgttgtagta gccggtgtga tcgccgaacg gtccttcggg
      181 cgccgtgtcg ccgggcgcga tgtggccttc gagcacgaac tcggcgctgg ccggcacctg
      241 caggtcgcag ccgaggcagc gcgcgagttc ggtcttcgcc ccgcgcagca ggccggcgaa
      301 cgcgtattcg gacagggtat ccggcaccgg cgtcacggcc ccgaggatgg tggccggatc
      361 ggccccgagc gccaccgcca ccggaaacgg ccggcccggg tgcgcctgct gccattcgcg
      421 gaagtcgagc gcaccgccgc ggtgcgacag ccagcgcatg atcacgcggt tgcggccgat
      481 cacctgctgg cggtagatgc cgagattctg ccgggccttg tgtggcccct tcgtgaccac
      541 cagcccccag gtgatcagcg gtccggcatc gccgggccag caggtctgta ccggcaggcg
      601 cgcgagatcc accgcctcgc cttcgagcac gcattcctgg cacggcgggc gcgagatctc
      661 cttcggtgcc atgtcgagca ccttgcggaa caccggcagg ctgttccagg cgtccttcag
      721 gcccttcggc ggctccggct ccttcaggaa cgcgagcagc ctgccgacct cgcgcagcgc
      781 cgcgaccgac tccgcgccca tgccgagcgc gacgcgctcc ggtgtgccga acaggttcgc
      841 cagcaccggc accgagtgcc cggccggacg ctcgaacagc agcgccggcc cgcgcgcgcg
      901 cagcacgcga tcgcagatct cggtcatctc cagccgcggc gacaccggca cgcggatgcg
      961 ccgcagctgg ccatcggctt cgaggcgggc aatgaagtca cgcaggtctt tgtatttcat
     1021 cgggactcga cgacctgacg gtcatccgga cgacgggggg aagcggcggc gggcgtgccg
     1081 gcagccgcgc ggagccgact ctacactagg ccggtcgccg gcagcgtgat ccgcgaaagc
     1141 gccggtatga ccatttgacc agaacagtgt aagctgcgcg gactccgcga cccggagccc
     1201 atccccataa aagcccgcac ggcacccccg gcccgccccc cacgcgcgcc ggcaagccgg
     1261 cgcacgaccg gaggagaaac aaagggtgga cttcctgctg agactttccc gtggcatcga
     1321 ctggctcaat gagcacgtcg gcaagaccgt gtactggctg atcctgatca tggtgatcat
     1381 ctgcacgggc aacgcgctga tgcgcaagct gttcaacatc agctcgaacg gcttcctcga
     1441 aatccagtgg tacctgttcg cggcggtgtt cctgctcggt gccgcctaca cgctgcagcg
     1501 caacgagcac gtgcgcatcg acgtggtcgt cgggcgcttc agcctgcgca cgcagatcat
     1561 catcgacatc ctcggcacgc tgctcttcct gacgccgctg tgcctgctga tgatcgtgtt
     1621 cggctggccg tacttcctcg attcctggca cacgcaggaa tactcgccga accccggcgg
     1681 cctgatcctc tggccggtga aggtcctgat cccgatcggt ttcgtgctgc tgcagctgca
     1741 gggcctgtcg cagctgatca agcagatcgg cgcgctgacc ggacacgtcg atccgcacac
     1801 gctggtcaag tcgcaccacg gccacgcgcc cgccgacgtg cccgcgccgc acaccccgaa
     1861 ctgacggagc gcgcccccga tggaagcctt cattgttgcc aacatggcgc cgctgatgtt
     1921 cggcgccctc gtgatcttcc tgctgctcgg ctatccggtc gcgttcgcgc tggcggccaa
     1981 cggcatggtc ttcggcctga tcggcatcga atacggactg ctgaagcccg agctgctgca
     2041 ggcgctgccg cagcgcgtgt tcggcatcat gcagaacgac acgctgctcg cgatcccgtt
     2101 cttcacgttc atgggcctga tcctcgaacg ctcgggcatg gccgaggatc tgctcgacac
     2161 catcggccag ctgttcggcc cgatccgcgg cggcctcgcc tacgcggtga tcttcgtcgg
     2221 tgcgctgctc gcggcgacca ccggcgtggt cgcggcctcg gtgatctcga tgggcctgat
     2281 ctcgctgccg atcatgctgc gctacggcta cgacaagaag ctggcgaccg gcgtcatcgc
     2341 cgcttccggc acgctcgcac agatcatccc gccgtcgctg gtgctgatca tcatggccga
     2401 ccagctcggc cgttcggtcg gtgacatgta tgccggtgcg ctgattccgg gcctggtgct
     2461 gaccggcatg tacgtcggct acgtgttcct gaccgcgacc ttcaagccgg gcgcggcacc
     2521 agcgctgccg cccgaggccc gcagcctgcg cggcgcggcg ctcgggcggc gcgtgctggt
     2581 gtcgctggtg ccgccgctgg tgctgatctt cctcgtgctc ggcacgatct tcatcggcgt
     2641 cgcgacaccg accgaaggcg gcgcgatggg tgcctcgggc gcgatcatcc tggcgctgat
     2701 caaccgccgc ctcgactgga agctcgtgcg tcaggcgctc gaccagaccg ccaagctgtc
     2761 gtgcttcgtg ctgttcatcc tgatcggctc gacggtgttc ggtctggtgt tccgcgccgt
     2821 caacggcgat ctgtgggtcg agcacctgct gacggcgctg ccgggcggcg aggtcggctt
     2881 cctgatcgtc gtcaacatcc tgatgttcgc gctggccttc ttcctcgact tcttcgagct
     2941 ggccttcatc ctcgtgccgc tgctcggccc ggtcgccgag aagctcggca tcgacctgat
     3001 ctggttcggc gtgctgctcg gcgtcaacat gcagacgagc ttcatgcacc cgccgttcgg
     3061 cttcgcgctg ttctacctgc gctcggtcgc gccgcccgac atccgcacca cacacatcta
     3121 ctggggtgcg gtgccgttcg tgtgcatcca gatcatcatg gtggcgctgc tgatcgcctt
     3181 cccgggtctg gtcagcgtcg agaagcacaa ggtcgacaac agcgccccgg tgcagatcga
     3241 gatgccggcc gtcgactacg gcgccccggc cggcgacggt ggctacgcgc cgccgagcta
     3301 cgactatcag ccggccccgc cgcccgagcc ggccaaaccc tgagcggccc gctccccgct
     3361 gccgccgatc cgggcggcag cggggaaaac agccgcgaag acccaagaaa aagccccgcc
     3421 ggatggcggg gctttttgct gcctgtgctg cgacttacag gcgctggctg tagacgaagt
     3481 tcgcgtaggt cgcctcggcg acgcggaacc actggtactc ggccgaacgg aaggtcttcc
     3541 aggagtcgta gagcttcttg aactgcgggt tcttgccggc ctcttcctca tacagctcgt
     3601 tggccgtctt gaacgccgct tccatgacat cgcgcgggaa cgggtgcagc ttggcgccgt
     3661 cgcgcaccag ccggctcagc gccggcgggt tcttggcgtc gtacttggcc atcatgtcga
     3721 tgttggcctc ggccgccgcc gccgtgaacg ccgcctggta gctcttcggc agcttgtccc
     3781 attcgccctg gttgacgaag aagctgacgt tcggcccgcc ttcccaccag ccggggaagt
     3841 aataatgcgg cgcgaccttc acgaagccga gcttctcgtc gtcgtacggc ccgatccatt
     3901 cggccgcatc gatcgtgccc ttttccagcg acgggtagat gtcgccaccc ggaatctgct
     3961 gcggcacggc gccgaggcgc gacatgatct cgccgccgag gccggcaatg cgcatcttca
     4021 ggcccttgac gtcggccagt cccttgattt cgttgcggaa ccatccgccc atctgcgtgc
     4081 cggtgttgcc gcccggaaag ctgatgacgc cgtagtcctt caggaactcg cggtacagct
     4141 ccagaccgcc gccgtgatac agccaggcgt tctgctggcg gcaggtcagg ccgaacggca
     4201 gcgcggtgtc gaaggcgaag gctttgttct tgccgatgaa gtagtacatc gtcgcgtggc
     4261 cgcactcgac cgtgccctgc tgggcggcgt cgagcgtctg gaagccgggg acgatctcgc
     4321 cgccggcgaa cacgcggatg tcgaacttgc cttcggtgat ctcgcgcacg cggttggcga
     4381 gcacctcggc ggcgccgtag atcgtgtcga ggctcttcgg gaagctcgat gccagtcgcc
     4441 atttgatctg cggttcggcg cgcacgatcg ccggggcggc aacggtcgcg gcggcggcca
     4501 gaccggcacc ggcggtcttc aggaagtcgc gtctctccat tttcttctcc tcgctttatc
     4561 aggggggccc atggcccggg ccagtatagg cagtgcatcc ggactgccag cgccaagcct
     4621 gacggattcc ggaaacgaaa cggggctgct cacgcagccc cgtcgcccga ctggtccgac
     4681 aataagacca actcagttgg cggccggcaa gaccttgact cccttctgcg cgaagcgctc
     4741 gttgtcgagg tagacctcga cctgccactg cccgggctgc tgcaggtagc ggttgatctg
     4801 gaagctgctg acggtcgagc ggctgacgtt gtcggccgtg aagcgctggg catccgagaa
     4861 caccggcttg cgctcgccgt cgagcacgcg caccagctgc gtgtgctcgc ccggggcgag
     4921 ctggttccac tgcgtgaaca cgtggaagcg ctcctgatcg aggctcaggg cctcgacggc
     4981 gttgaccggc tgctggcgat cgttgaggcc ggtggtcagc acgacggtcg cgctggtgcg
     5041 gaaggacggg gcggtggccg gagtgccgca ggcgcccgac aggagcgcag cggccaggaa
     5101 caggatgcgg attttcatga cggcgaaact cgatggggcg ggcggggcgg tgacggccgg
     5161 gccggcacta taccgcagcg tccgcagccg gccggctcag gtgtcgaagc tcgccagcac
     5221 cggcgcgtgg tcggacggcc gctccagccg ccgcggcccg gtgtcgatcc ggcagccacg
     5281 gcagcgcgcc gcgagcgccg gcgacagcag gatgtgatcg atgcgcagac cgtgattgcg
     5341 gcggaagccg ccgccgcggt aatcccacca cgagaacgtc ttcggcggct gctcgaacag
     5401 ccggaacgca tcgcggaagc ctgcatcgag caggccgcgg aaggcggcac gctcgggccc
     5461 ggagcacagg atctgtccgc gccaggcttc ggggtcatgc acgtcgcgat cctcgggcgc
     5521 gatgttgaag tccccgagca cgacgagttc gggatgcgcg gcacgctcgg tttcgagaaa
     5581 ggccgcgagc ttcgccagcc agtcgagctt gtaggcgtac ttgtcgctgc cgaccgcctg
     5641 accgttcggc acgtacagat cgagcacgcg cacgccagcg accgtggccc cgagcacgcg
     5701 ccgctgcggg tccgcgagcc cgggcagctc ggtcaccacc gcgtctgccg gctcgcgcga
     5761 cagcagcgcg acgccgttgt aggtcttctg gccggcgaag acgacctcgt agccggcggc
     5821 gcggatcgcc ccggccggga aatcggcgtc ctgcagcttg gtttcctgca gcgccagcac
     5881 gtccgggctt tcggcggcca gccagtcgag cacctgcggc agccgcacct tcagcgagtt
     5941 gacgttccag gtggcgatct tgcgcatcgg cggcttccgg tcggggacgg gggcgcagca
     6001 tacaatggcg cccttccccc gccgtccgtc cggatgccgc ccgcatgaga attccccgca
     6061 tccacgtcgc cggtccgatc gtcgccggcg agccggtcgc gctcgatgac agcgccagca
     6121 accacgtcgt gcgcgtgctg cgcctcgcgc cgggcgcgcc gctgcggctg ttcgacggcc
     6181 gcggcggcga gtggcgggcg acgttggcga gctgcggc
//
LOCUS       DAOQJH010000047         6126 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_7271_length_6126_cov_4.540109, whole genome shotgun sequence.
ACCESSION   DAOQJH010000047 DAOQJH010000000
VERSION     DAOQJH010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 6126)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6126)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6126
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7271_length_6126_cov_4.540109"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="PLQ18_01965"
     CDS             complement(<1..219)
                     /locus_tag="PLQ18_01965"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442392.1"
                     /note="catalyzes the formation of 4-aspartyl phosphate
                     from aspartate 4-semialdehyde; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HNE00445.1"
                     /translation="MRRVGLVGWRGMVGSVLMERMRAENDFALIDPVFFTTSNVGGSG
                     PDIGKPIAPLADARSVEALTAMDVIISCQ"
     gene            complement(336..1412)
                     /gene="leuB"
                     /locus_tag="PLQ18_01970"
     CDS             complement(336..1412)
                     /gene="leuB"
                     /locus_tag="PLQ18_01970"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020165438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HNE00446.1"
                     /translation="MTKKILVLAGDGIGPEIVGEAVRVLEVLRSGYGLDVELDTALLG
                     GCAVDATGEPYPDATRAKAQAADAVLLGAVGGPQYDTLPRPQRPERGLLAIRKDLNLF
                     ANLRPAVVYPQLAAASSLKPELVAGLDILIVRELTGDIYFGEPRGIELRDGERVGWNT
                     MIYSESEIRRIVRVAFDAARKRGGRVCSVDKMNVLECTQLWRDVAEEVAKDYPDVQLT
                     HMLVDNAAMQLVRNPKQFDVMVTGNMFGDILSDEASMLTGSIGMLPSASLDEHGKGLY
                     EPIHGSAPDIAGKGVANPLATILSVAMMLRYSLGRADLAERVERAVSRVLDQGLRTAD
                     IRAEGCETVGTLAMGEAVLAALAD"
     gene            complement(1460..2011)
                     /locus_tag="PLQ18_01975"
     CDS             complement(1460..2011)
                     /locus_tag="PLQ18_01975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE00447.1"
                     /translation="MSSIEMAVRNARAIETLLVERLGANGKGLHEKLNSVERQLPPHI
                     VKTARWIATLRNSAVHQHDFEISNPDDFMNAAARVLAHLESMPKTAGAAAAAQAAESS
                     RSGSMSGGLRLETVRASRPQPGAHQGGSAPAAVPARTARRRRQPEARSSGQGWWLAFV
                     LVAGGLLVAALFKSGALRSIVSG"
     gene            complement(2125..2514)
                     /locus_tag="PLQ18_01980"
     CDS             complement(2125..2514)
                     /locus_tag="PLQ18_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006559685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNE00448.1"
                     /translation="MRLLLDTHALLWWFAGDERLPEKVRSFIADRQNAVLVSAASIWE
                     IATKHRLGKLEGVGELLADIDTYVRDQSFDWLDIAAVHALRAGLLPGAHRDPFDRMLI
                     AQALCVEAVLVSNETLFDGFGVQRLWS"
     gene            complement(2514..2750)
                     /locus_tag="PLQ18_01985"
     CDS             complement(2514..2750)
                     /locus_tag="PLQ18_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017926937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HNE00449.1"
                     /translation="METCPVHEAGEHLAVLIERAWRGEDIIITGEAAQAVKLVPVTTR
                     AAGQRQFGRLRGRIRVDDAFFDPLPEEVLAAWER"
     gene            complement(2794..3432)
                     /gene="leuD"
                     /locus_tag="PLQ18_01990"
     CDS             complement(2794..3432)
                     /gene="leuD"
                     /locus_tag="PLQ18_01990"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HNE00450.1"
                     /translation="MQPFTELHGLVVPLDRPNVDTDAIIPKQFLKSIKRSGFGPNLFD
                     EWRYLDVGEPGQDCAGRPRNPDFSLNQPRYAGASVLLARENFGCGSSREHAVWALEDY
                     GIRCVIAPSFADIFFSNCGKNGVLAIRLPAETVDRLFRAVEATPGYQLFVNLPGQTIA
                     TPDGEVIDFDIDAFRKECLVNGWDDIGLTLRHAGDIRAYEAKRRQEAPWLFQ"
     gene            complement(3457..4869)
                     /gene="leuC"
                     /locus_tag="PLQ18_01995"
     CDS             complement(3457..4869)
                     /gene="leuC"
                     /locus_tag="PLQ18_01995"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HNE00451.1"
                     /translation="MSGQTLYDKIWAAHVVRTDADGTALLYIDRHLVHEVTSPQAFEG
                     LKLAGRAPWRRASMLAVPDHNVPTTPRAGGIGDIEDPVSRTQVETLDANARELGLAYF
                     PINDVRQGIVHVIGPEQGATLPGMTVVCGDSHTSTHGAFGALAFGIGTSEVEHVMATQ
                     CLIQKKAKAMLVRVDGEAGPGVTAKDIVLAIIGRIGTAGGTGYAIEFGGSAIRALSME
                     GRMTVCNMAIEAGARAGMVAVDATTLGYVQGRPYAPKGADWDKAVAWWNTLHSDADAR
                     FDAVVELDAAAIAPQVTWGTSPEMVVAVGENVPDPAAETDTVKKSGMEKALAYMGLAA
                     RQPVTDIRLDRVFIGSCTNSRIEDLRAAAAVIRGRKVAASVKQALVVPGSGLVKQQAE
                     AEGLDKLFLDAGFEWREPGCSMCLAMNADRVGPGEHCASTSNRNFEGRQGAGGRTHLV
                     SPAMAAAAAIAGHFVDVRSF"
     gene            4998..5906
                     /locus_tag="PLQ18_02000"
     CDS             4998..5906
                     /locus_tag="PLQ18_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNE00452.1"
                     /translation="MDIASLQAFVAVADSGSFSVAAERLFLTQPAVSKRISALEAELG
                     VRLFDRLGRRVLVTEAGGTLLPRARRILAELDDSRRALADLSGQVAGRLSVATSHHVG
                     LHALPPVLRAYTASYPQVQLDLRFMASEAACLAVAGGDVELAIVTLPLAAEAPLALQT
                     IWTDRLVLVAHPGHPLARLKRVSPFRLVEHAAILPEHGTFTRELIERCFEPLGIRLRV
                     AFTTNFLETIKMMVSIGLGWSLLPRTMLDAQLAVLELEGIAPTRALGLVAHAGHTPSN
                     AARALLGLLAPGEGGGPAEPEPWRGY"
BASE COUNT          917 a         2215 c         2013 g          981 t
ORIGIN      
        1 ctgacaggag atgatcacgt ccatcgccgt cagcgcctcg accgagcgcg catcggcgag
       61 cggcgcgatc ggcttgccga tgtcggggcc gctgccgccg acgttcgagg tcgtgaagaa
      121 caccggatcg atcagcgcga agtcgttctc cgcgcgcatc cgctccatca gcacggaacc
      181 gaccatgccg cgccagccca ccagacccac acgtctcatt gccttgcctc tttcgttcgg
      241 gtgacggcgg accacgcagc cgccgcaaca cattgaatca tcaatcctgt gccggcgccc
      301 gcctccagcg gaggcaggca ccggcgttcc gggcgtcagt ccgcgagcgc ggccagcacg
      361 gcttcgccca tcgccagcgt gccgaccgtc tcgcagcctt cggcgcggat gtcggcggtg
      421 cgcaggccct ggtcgagcac gcgcgacacg gcgcgctcga cgcgctcggc cagatcggca
      481 cggccgagcg agtagcgcag catcatcgcc accgacagga tggtcgcgag cggattggcc
      541 acgcccttgc cggcgatgtc cggagccgag ccgtgaatcg gctcgtacag gcccttgccg
      601 tgctcatcga gcgaggccga cggcagcatg ccgatgctgc cggtcagcat cgaggcctcg
      661 tccgagagga tgtcgccgaa catgttgccg gtgaccatca cgtcgaactg cttggggttg
      721 cgcaccagct gcatcgcggc gttgtcgacg agcatgtgcg tcagttgcac gtccgggtag
      781 tccttcgcga cctcctcggc cacgtcgcgc cagagctgcg tgcattcgag cacgttcatc
      841 ttgtcgaccg agcagacccg gccgccgcgc ttgcgggcgg catcgaaggc gacgcgcacg
      901 atgcggcgga tctcggattc cgagtagatc atcgtgttcc agccgacgcg ctcgccgtcg
      961 cgcagctcga tgccgcgcgg ctcgccgaag tagatgtcgc cggtcagctc gcgcacgatc
     1021 aggatgtcga gcccggccac cagctccggc ttcagcgacg aggccgccgc gagctgcgga
     1081 tagaccacgg ccgggcgcag gttcgcgaac aggttcaggt ccttgcggat cgcgagcagc
     1141 ccgcgctccg ggcgctgcgg gcgcggcagc gtgtcgtact gcggaccgcc gacggccccg
     1201 agcagcacgg catcggcggc ctgcgccttc gcgcgcgttg cgtccgggta cggctcgccg
     1261 gtggcatcga cggcgcagcc gccgagcagc gcggtgtcga gctcgacatc gaggccgtag
     1321 ccgctgcgca gcacttcgag cacccggacg gcctcgccga cgatctccgg accgatgccg
     1381 tcaccggcga ggacgagaat cttcttggtc atgccctgat cacctctgtt gaatcgttgc
     1441 tgcaaccgcc cggggttgct catccgctga cgatcgaccg cagggcgccc gacttgaaca
     1501 gcgcagccac gagcagcccg ccggccacca gcacgaaggc cagccaccag ccctgcccgc
     1561 tgctgcgtgc ctcgggctga cgccggcgcc gcgccgtgcg cgccggcact gctgccggtg
     1621 cggatccgcc ctgatgtgcc cccggctgcg gacgcgacgc ccggacggtt tccagacgca
     1681 gcccgccgga catcgacccg gacctcgacg actccgctgc ctgcgccgcc gctgcagccc
     1741 cggctgtctt cggcatcgac tccagatgcg cgagcacgcg cgcggccgca ttcatgaagt
     1801 cgtccggatt gctgatctcg aaatcgtgct ggtgcacggc cgagttgcgc agcgtcgcga
     1861 tccagcgcgc ggtcttgacg atgtgcggcg gcagctgtct ttcgacgctg ttcagcttct
     1921 catgcaggcc cttgccgttc gccccgagcc gctcgaccag cagggtttcg atcgcccggg
     1981 cgttgcgtac cgccatctcg atgctcgaca tccctcgcct cctgaatccg ccatcggtcg
     2041 gccgcgcatc atgccgcgag cggctgccgg cattcgagtc cgtcgtcacc gctgacaacc
     2101 ggccgcctct cccacgcctt gccctcagct ccacagccgc tgcacgccga agccgtcgaa
     2161 cagggtttca ttgctgacca gcacggcctc cacgcacagg gcctgggcaa tcagcatccg
     2221 gtcgaacgga tcccggtgcg cacccggcag caatccggct cgcagtgcat ggaccgccgc
     2281 gatgtcgagc cagtcgaagg actgatcacg cacgtaagtg tcgatgtccg ccagcaactc
     2341 acccacgccc tccagctttc caagccggtg cttcgtcgcg atctcccaga tcgaggcagc
     2401 actgaccagc acggcattct gtcggtcggc gatgaatgag cggactttct ccggcagacg
     2461 ctcatccccg gcgaaccacc agagcagggc atgtgtatcg agcagcagac gcactagcgc
     2521 tcccaggcgg cgaggacttc ttccggcaaa gggtcgaaga acgcgtcatc gacccggatt
     2581 ctcccgcgca accgaccgaa ttgacgctga ccagccgcac gggttgtcac cggtacgagc
     2641 ttcacggcct gtgccgcctc accggtgatg atgatgtcct caccacgcca ggccctctcg
     2701 atgagaaccg ccagatgctc acccgcttca tgaaccggac aggtttccat cgcagacctc
     2761 cggacgccgg aattcagccg ataccaactt gactcactga aacagccacg gcgcttcctg
     2821 ccgccgcttc gcctcgtagg cgcggatgtc accggcgtgt ctgagcgtca ggccgatgtc
     2881 gtcccagccg ttgaccaggc attccttgcg aaacgcatcg atgtcgaagt cgatgacctc
     2941 gccatcgggc gtggcgatcg tctggcccgg caggttcacg aacagctggt agcccggcgt
     3001 cgcttcgacc gcccggaaca gccggtcgac ggtctcggcc ggcaggcgga tcgccagcac
     3061 gccgttcttg ccgcagttgc tgaagaagat gtcggcgaag ctcggcgcga tcacgcagcg
     3121 gatgccgtag tcctccagcg cccagaccgc gtgctcgcgc gagctgccgc agccgaagtt
     3181 ctcgcgcgcg agcagcacgc tcgcgcccgc gtagcgcggc tggttcaggc tgaagtccgg
     3241 attgcgcggc cggccggcgc agtcctgacc cggttcgccg acatcgaggt agcgccattc
     3301 gtcgaacagg ttcgggccga agcccgagcg cttgatcgac ttcaggaact gcttcgggat
     3361 gatcgcgtcg gtgtcgacgt tcggccggtc gagcgggacg acgaggccgt ggagttcggt
     3421 gaacggctgc atggccatct cctcagaaat cttcgctcag aagctgcgca cgtcgacgaa
     3481 atgaccggcg atcgcggccg ccgcggccat cgccgggctg accagatgcg tgcgtccgcc
     3541 ggcgccctgg cggccctcga agttgcggtt cgaggtgctc gcgcagtgct cgccggggcc
     3601 gacgcgatcg gcgttcatcg ccagacacat cgagcagccc ggctcgcgcc attcgaagcc
     3661 ggcatcgagg aacagcttgt cgagcccctc ggcctcggcc tgctgcttca cgaggcccga
     3721 acccggcacg accagcgcct gcttgacgct cgcggcgacc ttgcggcccc ggatgacggc
     3781 ggcggcggcg cgcaggtcct cgatgcgcga gttcgtgcac gagccgatga acacgcgatc
     3841 aagccggatg tcggtgaccg gctggcgggc ggccagtccc atgtaggcga gcgccttctc
     3901 catgccggac ttcttgacgg tgtcggtttc ggcggccgga tcgggcacgt tctcaccgac
     3961 cgcgaccacc atctccggcg aggtgcccca ggtcacctgc ggcgcgatgg cggcggcgtc
     4021 gagttcgacg acggcatcga agcgcgcatc ggcatcggag tgcagcgtgt tccaccaggc
     4081 gacggccttg tcccagtccg cgcccttcgg cgcgtacggg cggccctgca cgtagccgag
     4141 cgtcgtcgca tcgaccgcga ccatgccggc acgggccccg gcctcgatcg ccatgttgca
     4201 gaccgtcatg cggccttcca tggacagcgc ccggatcgcg ctgccgccga attcgatcgc
     4261 gtaaccggtg ccgccggcgg tgccgatgcg gccgatgatc gcgagcacga tgtccttggc
     4321 ggtgacgccg gggccggcct cgccgtcgac gcgcaccagc atcgccttcg ccttcttctg
     4381 gatcaggcac tgcgtggcca tcacgtgctc gacctcggag gtgccgatgc cgaaggccag
     4441 cgcaccgaag gcgccgtgcg tcgaggtgtg cgaatcgccg cagacgacgg tcatgcccgg
     4501 cagcgtcgcg ccctgctcgg ggccgatcac gtgcacgatg ccctgacgca cgtcgttgat
     4561 cgggaagtac gcgaggccga gttcgcgggc gttggcgtcg agcgtctcga cctgcgtgcg
     4621 cgagaccggg tcctcgatgt cgccgatgcc accggcgcgc ggcgtggtcg gcacgttgtg
     4681 atcgggcacg gccagcatcg acgccctgcg ccagggcgcg cggccggcga gcttcaggcc
     4741 ctcgaaggcc tgcggcgagg tcacctcgtg cacgaggtgg cgatcgatgt acagcagcgc
     4801 ggtgccgtcg gcgtcggtgc gcacgacgtg cgcagcccag atcttgtcgt agagcgtttg
     4861 gccggacatt tcagcgtcct cgttcgggca gtgcgcggac tgtacgccgg gcctgaacat
     4921 gacaccaatt catgtttttc atactcggca tgacgccatg gaatacttgc acgcccccct
     4981 cccgcccggt gccgcgcatg gacatcgcca gcctgcaggc cttcgtcgcc gtcgccgaca
     5041 gcggctcgtt ctcggtcgcg gccgaacggc tgtttttaac gcaaccggcg gtcagcaagc
     5101 gcatctcggc gctcgaagcc gaactcggcg tgcggctgtt cgaccgcctt ggccggcgcg
     5161 tgctggtcac cgaagccggc gggacgctgc tgccgcgggc gcggcgcatc cttgccgagc
     5221 tcgacgacag ccgccgcgcg ctcgccgacc tgtccgggca ggtcgccggg cgcctgagcg
     5281 tcgccaccag ccaccacgtc ggcctgcacg cgctgccgcc ggtgctgcgc gcctacacgg
     5341 cgagctatcc gcaggtgcag ctcgacctgc gcttcatggc ctccgaagcc gcctgcctgg
     5401 cggtcgccgg cggtgatgtg gagctggcga tcgttacgct gccgcttgcc gccgaagccc
     5461 cgctggcgct gcagacgatc tggaccgacc ggctggtgct ggtggcgcac cccggccatc
     5521 cgctcgcccg cctcaagcgc gtgtcgccgt tccggctggt cgagcacgcg gcgatcctgc
     5581 ccgagcacgg caccttcacg cgcgagctga tcgagcgctg cttcgaaccg ctcggcatcc
     5641 ggttgcgcgt ggcgtttacg acgaacttcc tcgaaaccat caagatgatg gtgtcgatcg
     5701 gactcggctg gtcgctgctg ccgcgcacga tgcttgatgc gcagcttgcc gtgctcgaac
     5761 tcgaaggcat cgcaccgacc cgcgcgctcg gcctcgtcgc gcacgccggc cataccccgt
     5821 cgaacgccgc gcgcgcgctg ctcgggctgc tcgcgcccgg cgaaggcggc gggccggccg
     5881 agcccgaacc gtggcgcggc tactgaacgg gcagcggttc acgctgttat gctttccggc
     5941 ttgccgttcg gcacccgccg taccggatcc cgtagctgcc gcctgccgcc catgaacaag
     6001 accgaccgcg aacgcaccat cgcccttgcc gccatcttcc aggcggccgc gctggtgcgc
     6061 gatgtcgcct acgccggcca ggccgatgcc gccgacagcg aagcctgcct gcgcagcatc
     6121 ttcgcg
//
LOCUS       DAOQJH010000048         6106 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_7306_length_6106_cov_4.064287, whole genome shotgun sequence.
ACCESSION   DAOQJH010000048 DAOQJH010000000
VERSION     DAOQJH010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 6106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6106
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7306_length_6106_cov_4.064287"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..583
                     /locus_tag="PLQ18_02005"
     CDS             <1..583
                     /locus_tag="PLQ18_02005"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013395128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HNE00453.1"
                     /translation="DASGNCVRLIERVVEADEGPRADTTAAALAKLKPSFALRGSVTA
                     GNSSQMSDGAAAVLLVSGALLKELRLRPLARFVGYAVSGVAPEIMGIGPIAAVPKVLE
                     QTGIAPASLDWIELNEAFAAQALAVIHELGLDPAKVNPLGGAIALGHPLGATGAIRTA
                     TLVHGLARTGGRYGLVTMCVGTGMGAAGVFERV"
     gene            657..1307
                     /locus_tag="PLQ18_02010"
     CDS             657..1307
                     /locus_tag="PLQ18_02010"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNE00454.1"
                     /translation="MTATPEFLHFLRTPAGRELAGQFTSQRLARHTLLSTPGDARDRV
                     FIVLGGRLRVYLADDERELTLAFLEAGDVYATHTPTFVRTVSACTLLMLDTPRFAELL
                     QRQPLLTPAIMRVLGRLLGNSVSIIENLAFRDVRTRLAHWLAGQARRHGQHIDGMLAV
                     PMPLTFADIAQLLGTTRQSVSAGIGELERAGLIVRRGRRLLLVPDAQALLRGPGGS"
     gene            1404..1772
                     /gene="cowN"
                     /locus_tag="PLQ18_02015"
     CDS             1404..1772
                     /gene="cowN"
                     /locus_tag="PLQ18_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(2)-fixation sustaining protein CowN"
                     /protein_id="HNE00455.1"
                     /translation="MTAPASTILSATTLSATTLATTLAATTGAATTLAADRYVSFRGI
                     DFDGHCRALIETIERYHAGEGAREPFWGWFLDLRRNDPAKRDDLLLLSSLVNPLRERF
                     EAHGDAEALALLERAEEECF"
     gene            1866..3032
                     /locus_tag="PLQ18_02020"
     CDS             1866..3032
                     /locus_tag="PLQ18_02020"
                     /inference="COORDINATES: protein motif:HMM:NF037955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNE00456.1"
                     /translation="MPARLALWYFAYFAAVGVFLPFWPVYLRELGFPAAAIGTLLALY
                     SASRLVAPHLWGWLADRSGRRMAIVRAGGAATVLCFNGVFAVGDSFAGQAAVMAVFCF
                     FWTAALPQFEAVTLAHLGRDAHRYGRIRLWGSIGFIVVAAGGGAVVAACGALVIPWAM
                     LAGLVAIFVAGLLVPEPPVARAQDSATPLGQLLRTKAVWALIATGFLQQAAHGPYYTF
                     FTLYLGEHGIGERTAGLLWALGVVAEVGLFAWLPHGLPRVGLRRLLQLAVALSVLRWL
                     LLGLAVEVWALLLVAQLLHAASFAAFHACTLQLVQQRFGPAHAGQGQALYSSLGTGAG
                     AAFGAWLAGTLWLPWGGALTFVVAAGCSALAWLLVQYGLHTLQTVDTPATNPGP"
     gene            3102..4697
                     /locus_tag="PLQ18_02025"
     CDS             3102..4697
                     /locus_tag="PLQ18_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014746534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNE00457.1"
                     /translation="MSRRQTAVVLALAALLAPPLAQAAKTLVYCSEGSPEGFNAQFYT
                     AGTTFDAASQPIYNRLTEFERGATTLVAGLAESWTISPDGLVYTFNLRKGVKFHTTAK
                     FRPTRDFNADDVLFSFQRQLDPNHPFHKVSGGSYEYFEGMDMPAVIDRIEKLDDHTVR
                     FTLKRPEAPFLANIAMDFASIMSAEYADAMLKAGTPEVIDLEPVGTGPFQRVAYQKDA
                     VIRYKANPDYWRGKAPIDNLVFAITPDASVRYAKLDANECQVMSYPNPADLDRMRQDP
                     KVELLSQPGLNIGYLAFNTKKKPFDDVRVRQALNFAVNKAAIIEAVYQGAGTVAKNPI
                     PPTIWSYNDAVQDYAYDPQRAKQLLQQAGLADGFETDLWAMPVQRPYNPNARRMAEMI
                     QADWAKVGVKAKIVSYEWGEYLKRLKAGDHQTALMGWTGDNGDPDNFLATLLSCEAAD
                     NGSNYAKWCFQPYQDLVTKARQVSDPAERTQLYGQAQVVFKEQAPWITIAHSVVYQPV
                     RKEVQGFRIHPFGLNLFYGVDLK"
     gene            4732..5742
                     /locus_tag="PLQ18_02030"
     CDS             4732..5742
                     /locus_tag="PLQ18_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011390110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNE00458.1"
                     /translation="MIGFLLRRGLLLLPTFLGVTAIAFALIHLIPGDPLEMMAGERGM
                     DPARHARMMAELGFDRPLWQQYLGYLGDLLHGDLGRSINTRQPVLGEFLTLFPATVEL
                     SLAAMLIATLVGLPVGILAAVRRGTLFDHGVMGVSLAGYSMPIFWWALLLILLFSVQL
                     GWTPVSGRISVMFYVEPVTGFLLIDTLLAGDVDAFLSALHHLVLPAIVLGTIPLAVIA
                     RMTRSAMLEVIREDYIRAARARGLSPLRVIGVHALRNALIPVVTVIGLQTGTLLAGAI
                     LTETIFAWPGVGKWLIDSIARRDYPAVQGGILLVAAVVMAVNLAVDLLYGLINPRIRH
                     GR"
     gene            5777..>6106
                     /locus_tag="PLQ18_02035"
     CDS             5777..>6106
                     /locus_tag="PLQ18_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015590836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE00459.1"
                     /translation="MPAGSFAAGSHEALQEIEAVLPAPPAPWRENWRHFRANPGAFAG
                     LIVIVATIVIAIFAPYLAPYPDQGRGNSNMQERLQAPSGEHIMGTDQQGRDVLSRVMF
                     GARIPLII"
BASE COUNT          849 a         2117 c         2125 g         1015 t
ORIGIN      
        1 cgatgcgagc ggcaactgcg tgcgcctgat cgagcgcgtg gtggaggccg acgaaggtcc
       61 gcgggccgac accacggccg cggcgctggc gaagctcaag ccctcgttcg cgctgcgcgg
      121 cagcgtcacg gccggcaatt cctcgcagat gtcggacggc gccgcggccg tgctgctggt
      181 ctccggggcg ctgctgaagg aactccggct gcggccgctg gcgcgtttcg tcggctacgc
      241 ggtcagcggc gttgcccccg agatcatggg catcggcccg atcgcggcgg tgccgaaggt
      301 gctggagcag accggcatcg cgcccgcgtc gctcgactgg atcgagctga acgaagcctt
      361 cgccgctcag gccctggccg tgatccacga actcggcctc gacccggcga aggtcaaccc
      421 gctcggcggc gccatcgccc tcggccaccc gctcggcgcc accggcgcca tccgcaccgc
      481 cacgctcgtg cacgggctcg cgcgcaccgg cggacgctac ggtctcgtga cgatgtgcgt
      541 cggcaccggc atgggggcgg ccggggtgtt cgagcgggtg tgagcacagg gcgcggcagg
      601 gacggacaag gctccggtac gctgcgccgg ttccttgtct gccgccggcc gccgccttga
      661 ccgccactcc cgagttcctg cacttcctgc gcacaccggc cggccgcgag ctggccggac
      721 agttcaccag tcagcgcctc gcgcgccaca cgctgctcag cacgccgggc gatgcccggg
      781 atcgcgtgtt catcgtgctc ggcgggcggc tgcgcgtgta tctggccgat gacgagcgcg
      841 agctgacgct cgccttcctc gaagccggcg acgtatacgc gacgcatacg ccgaccttcg
      901 tgcgcacggt cagcgcctgc acgctgctca tgctcgacac gccgcgcttc gccgaactgc
      961 tgcagcgcca gccgctgctg acgccggcga tcatgcgcgt gctcggacgc ctgctcggca
     1021 actcggtctc gatcatcgag aacctggcat ttcgcgacgt gcgcacgcgg ctcgcgcact
     1081 ggctcgccgg gcaggcccgc cgtcatggcc agcacatcga cggcatgctg gccgtgccga
     1141 tgccgctgac gttcgccgac atcgcgcaac tgctcggcac cacgcgccag agcgtctcgg
     1201 ccggcatcgg cgaactggaa cgcgccgggc tgatcgtgcg ccgcggccgg cgcctgctgc
     1261 tggtgccgga tgcgcaagcc ttgctgcgcg gaccgggcgg aagctgaaat tcaggcccgg
     1321 aaacgtcggc cggccgacgg acggatcgca gccgcacagg ggatgctgct cctccctttc
     1381 ccgccatccc ccggaacccg tccatgaccg cccctgccag cacgatcctg tctgcaacaa
     1441 ccctgtctgc aacgaccctt gcaacgactc tggccgccac gaccggggct gccacgaccc
     1501 tggccgccga tcgctacgtc tcgttccgcg gcatcgactt cgacggccac tgccgcgcgc
     1561 tgatcgaaac catcgagcgc taccacgccg gggaaggcgc acgggaaccg ttctggggct
     1621 ggtttctcga cctgcgccgc aacgatccgg cgaagcgcga cgacctgctg ctgctgtcga
     1681 gcctcgtcaa tccgctgcgc gaacgcttcg aagcgcatgg cgatgccgag gcgctggcct
     1741 tgctggaacg cgcggaggag gagtgcttct gagccggtgc catcggctgg tggcatgagg
     1801 aagccacggc agagcgcgcg cttccgctac gatgcgcgcc cctctgccga ctgcgcactg
     1861 tcgtcatgcc cgcccgcctt gccctctggt acttcgccta tttcgccgcc gtcggcgtgt
     1921 tcctgccgtt ctggccggtg tacctgcgcg agctgggctt cccggcggca gcgatcggca
     1981 cgctgctggc gctgtattcg gcctcgcggc tggtggcgcc gcatctgtgg gggtggctgg
     2041 cggatcgcag cgggcggcgc atggcgatcg tgcgggccgg cggcgcggcg acggtgctgt
     2101 gctttaacgg cgtgttcgcg gtcggcgaca gttttgccgg gcaggcggcg gtgatggcgg
     2161 tgttctgctt cttctggacg gcggcgctgc cgcagttcga ggcggtgacg ctcgcgcatc
     2221 tggggcgtga tgcgcaccgc tacgggcgca tccggctgtg ggggtcgatc ggcttcatcg
     2281 tcgtcgcggc cggcggcggg gcggtggtgg ccgcatgcgg ggcgctcgtg atcccgtggg
     2341 cgatgctggc cgggctggtc gcgatcttcg tggccgggct gctggtgccg gagccgccgg
     2401 tcgcgcgtgc gcaggattcg gcgacaccgc tcgggcagct gctgcgcacg aaggcggtct
     2461 gggcgctgat cgcgaccggc ttcctgcagc aggccgcgca cgggccgtac tacacgttct
     2521 tcacgctgta tctcggcgag cacggcatcg gcgagcgcac ggccgggctg ctgtgggcgc
     2581 tcggtgtggt ggccgaggtc gggctgttcg cgtggctgcc gcacgggctg ccgcgcgtcg
     2641 gcctgcggcg gctgctgcag ctggcggtgg cgctgagcgt gctgcgctgg ctgctgctcg
     2701 ggctggcggt cgaggtgtgg gcgctgctgc tcgtcgcgca gctgctgcat gcggcgagct
     2761 tcgcggcctt tcacgcctgc acgctgcagc tcgtgcagca gcgcttcggg ccggcgcacg
     2821 ccgggcaggg acaggcgctg tattcgagtc tcggcaccgg ggccggcgcg gcgttcggcg
     2881 cgtggctggc cggcacgctg tggctgccct ggggcggggc gctgaccttc gtcgtggcgg
     2941 ccggctgttc ggcgctggcc tggctgctgg tgcagtacgg cctgcacacg ttgcagacag
     3001 ttgacacccc ggcgacgaat ccgggcccct gaaccgcttt gctgcgtgcc actgggctac
     3061 gctgcgcgcc attcaccgtg ctgcggagtc gccagaccat gatgtcccgt cgtcagaccg
     3121 ccgtcgttct tgcccttgcc gccctgctcg cgccgccgct cgcccaggcc gcgaaaacgc
     3181 tggtctactg ctcggaaggc agtcccgagg gcttcaacgc gcagttctac acggccggca
     3241 cgaccttcga tgccgcctcg cagccgatct acaaccgcct gaccgagttc gagcgcggtg
     3301 cgacgacgct ggtcgccggg ctcgccgagt catggacgat ctcgccggat ggcctcgtct
     3361 acacgttcaa cctgcgcaag ggcgtcaaat tccacacgac ggcgaagttc aggccgacgc
     3421 gcgacttcaa cgccgatgac gtgctgttct cgttccagcg ccagcttgat ccgaaccacc
     3481 cgttccacaa ggtctcgggc ggcagctacg aatacttcga aggcatggac atgccggcgg
     3541 tgatcgacag aatcgagaag ctcgacgatc acacggtgcg cttcacgctg aagcgcccgg
     3601 aggcgccgtt tctcgccaac atcgcgatgg atttcgcgtc aatcatgtcg gccgaatacg
     3661 ccgatgcgat gctgaaggcc ggcacgcccg aagtcatcga cctcgaaccg gtcggcaccg
     3721 ggccgttcca gcgcgtcgcg taccagaagg atgcggtgat ccgctacaag gccaatcccg
     3781 attactggcg cggcaaggcg ccgatcgaca atctggtgtt cgcgatcacg cccgatgcct
     3841 cggtgcgcta cgccaagctc gacgccaacg aatgccaggt catgagctat ccgaatccgg
     3901 ccgatctgga tcgcatgcgt caggacccga aggtcgaact gctgagtcag ccgggcctga
     3961 acatcggcta tctggccttc aacaccaaga agaagccctt cgatgacgtg cgcgtgcggc
     4021 aggcgctgaa ttttgccgtc aacaaggctg cgatcatcga ggccgtctat cagggcgccg
     4081 gcacggtcgc gaagaacccg atcccgccga ccatctggtc gtacaacgac gcggtgcagg
     4141 actacgccta cgacccgcag cgcgcgaagc agctgctgca gcaggccggc ctcgccgacg
     4201 gcttcgagac cgacctgtgg gcgatgccgg tgcagcgccc gtacaacccg aacgcgcgac
     4261 gcatggccga gatgatccag gccgactggg cgaaggtcgg cgtgaaggcg aagatcgtca
     4321 gctacgaatg gggcgagtac ctcaagcgcc tgaaagccgg cgatcaccag acggcgctga
     4381 tgggctggac cggcgacaac ggcgacccgg acaacttcct cgcgacgctg ctgtcctgcg
     4441 aggccgccga caacggcagc aactacgcga agtggtgctt ccagccgtat caggacctgg
     4501 tcacgaaagc ccgccaggtc agcgatccgg ccgagcgcac gcagctctac ggacaggcgc
     4561 aggtggtgtt caaggagcag gcgccgtgga tcacgatcgc gcactcggtc gtctaccagc
     4621 cggtgcgcaa ggaagtgcag ggtttcagga ttcacccctt cgggctgaac ctgttctacg
     4681 gcgtggacct gaagtgatgc ggcctgcgca tccgcggccg gggcggccgg cgtgatcggc
     4741 ttcctgctgc gccgcgggct gctgctgctg ccgacctttc ttggcgtcac ggcgatcgcg
     4801 ttcgcgctga tccacctgat ccccggcgat ccgctggaga tgatggccgg cgagcgtggc
     4861 atggacccgg cgcgccacgc gcggatgatg gccgaactcg gtttcgaccg gccgctgtgg
     4921 cagcagtacc tcggctatct cggcgacctg ctgcacggcg acctcggccg ctcgatcaac
     4981 acccgccagc cggtgctcgg cgagttcctg acgctgtttc cggcgaccgt cgagctgtcg
     5041 ctcgcggcga tgctgatcgc gacgctggtc gggctgccgg tcggcatcct cgcggccgtg
     5101 cgccgcggca cgctgttcga tcacggcgtc atgggcgtgt cgctcgccgg ctattcgatg
     5161 ccgatcttct ggtgggcgct gctcttgatc ctgctgttct cggtgcagct cggctggacg
     5221 ccggtgtcgg ggcggatctc ggtgatgttc tacgtcgagc cggtcaccgg cttcctgctg
     5281 atcgacacgc tgctcgccgg cgatgtcgat gccttcctgt cggcgctgca ccatctcgtg
     5341 ctgccggcga tcgtgctcgg cacgattccg ctcgcggtga tcgcgcgcat gacgcgctcg
     5401 gcgatgctcg aagtgatccg cgaggactac atccgtgccg cccgcgcccg cggcctgtcg
     5461 ccgctgcgcg tgatcggcgt gcatgcgctg cgcaacgcgc tgatcccggt cgtgaccgtc
     5521 atcggcctgc agaccggcac gctgctcgcc ggcgcgatcc tgaccgagac catcttcgcg
     5581 tggccgggcg tcggcaagtg gctgatcgac tcgatcgcgc ggcgcgacta cccggcggtg
     5641 cagggcggca tcctgctggt ggcggcggtg gtgatggccg tgaatctcgc cgtcgacctg
     5701 ctctacggcc tcatcaaccc gcgcatccgc catggccgct gagcgtccgg gggagccaca
     5761 ggtcccggcg gctaccgtgc cggccggaag cttcgcggcc ggcagccacg aggcgctgca
     5821 ggaaatcgag gccgtgctgc cggcgccgcc ggcgccgtgg cgcgagaact ggcggcactt
     5881 ccgtgccaat cccggtgcct tcgccgggct gatcgtcatc gtggcgacca tcgtgattgc
     5941 gatcttcgcg ccgtatctgg cgccgtaccc ggaccagggg cgcggcaaca gcaacatgca
     6001 agagcgtctg caggcgccga gcggcgagca catcatgggg acggaccagc agggccgcga
     6061 cgtcctgagc agggtcatgt tcggcgcccg catcccgctc atcatc
//
LOCUS       DAOQJH010000049         6093 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_7327_length_6093_cov_4.385227, whole genome shotgun sequence.
ACCESSION   DAOQJH010000049 DAOQJH010000000
VERSION     DAOQJH010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 6093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6093
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7327_length_6093_cov_4.385227"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1612)
                     /locus_tag="PLQ18_02040"
     CDS             complement(<1..1612)
                     /locus_tag="PLQ18_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002930645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNE00460.1"
                     /translation="MARFFIDRPIFAWVISITIMLAGALAIFTLPIAQYPDIAPPTVA
                     INATYPGASAKTIEDSVTQVIEQNMKGIDGLQYMSSNSSSAGTARVELTFSADTNPDI
                     AQVQVQNKLQLALALLPQAVQSQGVTVTKSANSFLAVYAFVSDRDDVTGTDIADFVSN
                     QVLDPISRIDGVGETTLLGARYAMRIWLDPAKLVKYALTPADVKSAIEAQNTQVAVGQ
                     LGDLPAAPGTGLNATINARSRLQTPEQFRAIVLRSATDGATVRLSDVARVEVGAESYS
                     VLARFNGRPAAGLAIRLASGANALAVAEAAEAKIRELERFFPSGIRAVIANDTSPFVR
                     LSIEEVVKTLIEAVVLVFGVMYLFLQSWRATLIPTIAVPVVLLGTFGVLAALGYSINT
                     LTLFAMVLAIGLLVDDAIVVVENVERVMAEQKLSPLEATRRSMDQISGALIGIALVLS
                     AVFVPMAFIQSSSGVIYRQFSVTIVSAMALSVLVALTLSPALCATLLRPLPAGHHAGG
                     HGRLGRAFAAFNRGFGRTTDRYAAAAGGI"
     gene            complement(1629..2846)
                     /locus_tag="PLQ18_02045"
     CDS             complement(1629..2846)
                     /locus_tag="PLQ18_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006463234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNE00461.1"
                     /translation="MPQSRKSEWPLILGLVLPLSLVAVTLSGCDEPQAAVKPAAPAPV
                     EVGVVEIRPEPLTLTTELPGRTRAHLVAEVRPQVGGIIEQRLFREGAEVGAGDLLYRI
                     DPASFQAAYDSAKAALAKAQADIRSVQLKAERYKDLVRIKAVSDQDYDDARAALAQAQ
                     AEVEAQKAALETARINLDYTQVRAPIAGRIGRSSVTPGALVTADQTTALATIQQLDPV
                     YVDVQRSSTEWLELQQALKSGRLSHPGADGSVAVRLKLEDGSDYPQTGRLLFSEVTVD
                     AGTGAITLRAEFPNPDQRLLPGMYVRAVLEEGIAQQALLVPQRAVSRDARGKASALVV
                     NADGKAERRDLVTERAVGDRWLVTTGIAAGERVIVDNLQKVKAGALVKPLPWTAPKRE
                     TAGLRPLTDLDPI"
     gene            complement(3950..4084)
                     /locus_tag="PLQ18_02050"
     CDS             complement(3950..4084)
                     /locus_tag="PLQ18_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006158366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3149 domain-containing protein"
                     /protein_id="HNE00462.1"
                     /translation="MDTLKLLFSDWTGILSALVILFMLGMGVYFGWLFIGSSGDGPKS
                     "
     gene            complement(4254..5102)
                     /locus_tag="PLQ18_02055"
     CDS             complement(4254..5102)
                     /locus_tag="PLQ18_02055"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016877734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HNE00463.1"
                     /translation="MTLPPEDRSRRQFLSALAAAGFAAAVQPVCADTILTPTDGLDAG
                     MDRVAANDGFLPVYRAVPQGRKNLPLILVVQEIFGLHEHIKDICRRLARLGYCAIAPD
                     LYYRQGDATKVSDIPTLLKTIVAFVPDEQVLRDLDTVVRWAQGSGYYDGHRLGITGFC
                     WGGRITWMYCGHNPQVRAGVAWYGKLEGEETAMTPQHPINIADSLQVPVLGLYGGQDA
                     GIPQDSLGRMRSRLASAHSQSEIVIYPDAPHAFNADYRPSYRKAAAEDGWRRMREWFS
                     VHGVNV"
     gene            complement(5171..5974)
                     /locus_tag="PLQ18_02060"
     CDS             complement(5171..5974)
                     /locus_tag="PLQ18_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNE00464.1"
                     /translation="MAADAGTATAPAPAPLLEARGLSVEVGTHRVCTGLGFALRAGER
                     WGLLGANGSGKTTLLHTLAGLRAPQAGEVRLDGLPLTALAPRRRALRLGLLPQEHEDA
                     FPATVAETALTGRFPHLGRFGHAGAADRAAVEAALAATGLAAHAQRQVATLSGGERRR
                     LGFATLLAQDPAVLLLDEPTNHLDLGYQVRLLDALAARVAAEGRGWIMVSHDATLVAR
                     FCTHVLLVYGDGRTATGGPQLLCPQQLSALYGHPVLRLDGPLGPVFAPG"
     gene            complement(5964..>6093)
                     /locus_tag="PLQ18_02065"
     CDS             complement(5964..>6093)
                     /locus_tag="PLQ18_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011469676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HNE00465.1"
                     /translation="LVLADTLARTAIAPQQLPLGAVTALVGVPAFLWLLNRGRHGG"
BASE COUNT          952 a         2077 c         2180 g          884 t
ORIGIN      
        1 cgatgccgcc ggcggccgcg gcatagcggt cggtcgtgcg gccgaagccg cggttgaagg
       61 ccgcgaacgc gcgcccaagc cggccgtggc cgccggcgtg gtggcctgcg ggcagcgggc
      121 gcagcagcgt cgcacacagc gccggcgaca gcgtcagcgc caccagcacc gacagcgcca
      181 tcgccgagac gatggtcacc gagaactgcc ggtagatgac gccgctggaa ctctggatga
      241 aggccatcgg cacgaacacc gccgacagca ccagcgcgat gccgatcagc gcgccgctga
      301 tctggtccat cgaccggcgc gtggcttcga gcggcgaaag cttttgctcc gccatcacgc
      361 gctcgacgtt ctcgacgacg acgatggcgt catcgaccag caggccgatc gccagcacca
      421 tcgcgaacag cgtcagcgtg ttgatcgagt aaccgagcgc ggcgagcacg ccgaaggtgc
      481 cgagcagcac gaccggcacg gcgatggtcg ggatcagcgt cgcgcgccag ctctgcagga
      541 acaggtacat gacgccgaac accagcacga ccgcctcgat cagcgtcttg accacttcct
      601 cgatcgacag gcgcacgaac ggcgaggtgt cgttggcgat cacggcgcgg atgccggacg
      661 ggaagaagcg ctccagttcg cggatcttcg cctcggcggc ctcggcgacc gcgagtgcgt
      721 tggcgcccga ggcgagccgg atcgcgaggc cggcggccgg gcggccgttg aagcgcgcca
      781 gcaccgaata actttcggcg ccgacctcga cgcgcgcgac atcggacagg cgcacggtgg
      841 cgccgtcggt ggcgctgcgc agcacgatgg cgcggaactg ctccggcgtc tgcaggcggc
      901 tgcgggcgtt gatggtcgcg ttcagaccgg tgccgggcgc ggccggcagg tcgccgagct
      961 ggccgaccgc gacctgcgtg ttctgcgcct cgatcgccga tttcacgtcg gccggcgtca
     1021 gcgcgtactt cacgagcttc gccggatcga gccagatgcg catcgcgtac ctggcgccga
     1081 gcagcgtcgt ctcgccgacg ccgtcgatgc ggctgatcgg gtcgagcacc tgattggaaa
     1141 cgaaatcggc gatgtcggtg ccggtcacgt cgtcgcgatc ggacacgaag gcgtagacgg
     1201 cgaggaagct gttcgcggac ttggtgacgg tcacgccctg cgactgcacg gcctgcggca
     1261 gcagcgccag cgccagctgc agcttgttct gcacctgcac ctgggcgatg tcggggttgg
     1321 tgtcggccga gaaggtcagc tcgacgcgcg cggtgccggc ggacgaactg ttcgagctca
     1381 tgtactgcag cccgtcgatg cccttcatgt tctgctcgat gacctgcgtc accgagtcct
     1441 cgatggtttt ggccgaggcg ccgggatagg tggcgttgat cgcgacggtc ggcggcgcga
     1501 tgtcggggta ctgggcaatc ggcagcgtga agatcgccag cgcgccggcg agcatgatcg
     1561 tgatcgagat cacccacgcg aagatcgggc gatcgatgaa gaatcgggcc atggtcgcgc
     1621 gctccggctc agatcggatc gagatcggtc agcgggcgca ggccggcggt ttcccgcttc
     1681 ggcgccgtcc acggcagcgg cttcaccagc gcaccggcct tgaccttctg caggttgtcg
     1741 acgatcacgc gctcgccggc ggcgatgccg gtcgtcacca gccagcggtc cccgaccgcg
     1801 cgctcggtga ccagatcgcg gcgctcggcc ttgccgtcgg cattgaccac cagcgcgctg
     1861 gccttgccgc gcgcatcgcg gctgacggcg cgctgcggca ccagcagcgc ctgctgcgcg
     1921 atgccctctt cgagcaccgc gcgcacgtac atgcccggca gcaggcgctg gtccgggttc
     1981 gggaattcgg cgcgcagcgt gatcgcgccg gtgccggcat cgaccgtcac ctccgagaac
     2041 agcagcctgc cggtctgcgg gtaatcgctg ccgtcttcga gcttcaggcg cacggcgacg
     2101 ctgccgtcgg caccggggtg gctgagccgg ccgcttttca gcgcctgctg cagctccagc
     2161 cattcggtgc tggagcgctg cacatcgacg tagaccggat cgagctgctg gatggtcgcc
     2221 agcgccgtgg tctggtcggc cgtgaccagt gcgcccggcg tcaccgacga gcggccgatg
     2281 cgcccggcga tcggcgcgcg cacctgcgtg tagtcgaggt tgatgcgcgc ggtctcgagc
     2341 gcggccttct gggcctcgac ctcggcctgc gcctgcgcga gcgccgcacg cgcgtcgtcg
     2401 tagtcctgat cgctgaccgc cttgatgcgc accagatcct tgtaacgctc ggccttcagc
     2461 tgcaccgagc ggatgtcggc ctgcgccttg gcgagcgcgg ccttcgcgct gtcgtacgcc
     2521 gcctggaagc tcgccggatc gatgcggtac agcagatcgc cggcgccgac ctcggcgcct
     2581 tcgcggaaca gccgctgctc gatgatgccg ccgacctgcg ggcgcacctc ggcgaccaga
     2641 tgcgcgcggg tgcgtcccgg cagttcggtg gtcagcgtca gcggctcggg gcggatctcg
     2701 acgacaccga cctcgaccgg cgccggcgcc gccggcttca cggccgcctg cggctcatcg
     2761 cagccgctga gcgtgacggc gaccagcgac aacggcagca ccagcccgag gatcagcggc
     2821 cattcggatt tgcgggattg cggcatgggg ggaagtctcg ggcaggaccg gcggacacag
     2881 gggggaccgg cacggcaggg ttctcgttat ggattttttg atcatgaacc cggaaagccc
     2941 gttcaagttc aggcgattac acgctgttac acggttgcga cagccggcgg acacgtgcac
     3001 gcggccgctc gccgcaccgc taaacctgcg ccggctctgg ctttgtcgat cggcatgagg
     3061 ccggtgaagc ggctaactca cgattaaatc cgtatggctg atactggatg tctgcatagg
     3121 caggcatctg cgcagggctt gaggtccgtg caatgccgtg cggataccgg aagccgccgt
     3181 ggtggacctg cggggaggcc gcaagcggag ccggtggtgc gcatccggtc cgtccgccct
     3241 cggcgcctgc ccgcggaggc gcgcgtcgcg gcgctccggc gcaggcggca ggcaactgct
     3301 gcaaaccctt cagtttcagg tgtttcggcg cacaggcgcc gggccgttcg cggcatggcc
     3361 attgctgacc gcggaatccg gcacggtccg caatgcgcac tgatgtaccg ctcaggccga
     3421 tcccgcgtga atgcgtggtt tatcccccgc tctcccatcc cttcccggcg agggggaagg
     3481 gaatgaaatg accgggcatt caagtcgtga tgggtatcag acggctcatg gcattccgga
     3541 cccgccattc gggacgactc cggccggaca tctcccgcga aacgatatcc gttcttgcgc
     3601 tgcggcaatt gtccattcgc atcggatgac gatccggatg cggacgaccg atggcggcat
     3661 cggcgatttc ctgcccggaa gctcgccgga gattcactgg ggaatccgcc ggatcgcaat
     3721 cactgccaaa tcctgatgcg gaattaccgg aaaccaccgg ggcatggcgg tgaaggatgc
     3781 ggaaaccggc cggcaccgtc cggaaaccgg aagcggcgca ctgcaagcgg gaatgcatca
     3841 ggtccttgca tccggcggac gcgctcgaac gtccgtcgac cggcccgcat cgcgaaagcc
     3901 gggccggtcc cggaggatgc agcccgggca cgacggccgg cagcccggat caggacttcg
     3961 gcccgtcgcc cgaactgccg atgaacagcc agccgaaata cacgcccatg ccgagcatga
     4021 agaggatgac cagcgccgac aggatgccgg tccagtccga aaacagcagc ttcagggtgt
     4081 ccatcgcagg ctccgcgcag gggttggaaa tgcgcgtgag cgtccgcctg ccggctgcgg
     4141 accgccatgc ggattcgcag ccgcagcggc aggacgcgat cctcgcggga gcggatttcg
     4201 ggagcaacgg ccggcggaac cgcgtcctgc cggccggaca ggcagaccgg gcttcagacg
     4261 ttgacgccgt gcaccgagaa ccactcgcgc atgcgccgcc agccgtcctc ggccgcagcc
     4321 ttgcggtagc tcgggcggta gtcggcattg aacgcgtgcg gggcgtccgg atagatgacg
     4381 atctcggact ggctgtgcgc cgaggcgagg cggctgcgca tgcgcccgag cgaatcctgc
     4441 gggatgccgg cgtcctgccc gccgtacagg ccgagcaccg gcacctgcag cgagtcggca
     4501 atattgatcg ggtgctgcgg cgtcatcgcc gtctcctcgc cttcgagctt gccgtaccac
     4561 gccacgccgg cgcgcacctg cgggttgtgc ccgcagtaca tccaggtgat gcgcccgccc
     4621 cagcagaagc cggtgatgcc gagccggtgg ccatcgtaat aaccgctgcc ctgcgcccag
     4681 cgcacgacgg tatcgaggtc gcgcagcacc tgttcgtcgg gcacgaaggc gacgatggtc
     4741 ttcagcagcg tcgggatgtc gctgaccttg gtcgcgtcgc cctggcggta gtacaggtcg
     4801 ggcgcgatcg cgcagtagcc gagccgcgcc agccgccggc agatgtcctt gatgtgctcg
     4861 tgcaggccga agatctcctg cacgaccagg atcagcggca ggttcttcct gccctgcggc
     4921 acggcgcggt agaccggcag gaaaccgtcg ttggcggcga cgcggtccat gccggcatcg
     4981 aggccgtcgg tcggcgtcag gatggtatcg gcacagaccg gctgcaccgc tgcagcgaag
     5041 ccggcggcgg cgagcgcgct caggaactgg cgacgggagc gatcctcggg cggaagcgtc
     5101 atcggcgaaa ctcctgcaag gctggggaaa aagcgtgagg agccgggagc atagacgctg
     5161 cctgctgccc tcagcccggc gcgaacaccg gtccgagcgg tccgtcgagg cgcagcaccg
     5221 ggtgaccgta cagcgccgac agctgctgcg ggcacagcaa ctgcggcccg ccggtggcgg
     5281 tgcgaccatc gccgtagacc agcagtacat gcgtgcagaa gcgcgcgacc agcgtcgcgt
     5341 cgtggctgac catgatccag ccgcgcccct cggcggcgac gcgcgcggcc agcgcatcga
     5401 gcaggcgcac ctgataaccg aggtcgaggt ggttggtcgg ctcatcgagc agcagcacgg
     5461 ccgggtcctg cgccagcagc gtcgcgaagc cgagccgccg gcgctcgccg ccggacagcg
     5521 ttgcgacctg acgctgcgca tgcgcggcca gcccggtcgc ggccagcgcc gcctcgaccg
     5581 ccgcccggtc cgccgctccg gcgtggccga agcgcccgag gtgcggaaag cgcccggtca
     5641 gcgcggtttc ggcgacggtg gccgggaagg cgtcctcgtg ctcctgcggc agcaggccga
     5701 ggcgcagtgc gcgccggcgc ggcgcgagcg ccgtgagcgg cagaccgtcg aggcggacct
     5761 cgccggcctg cggcgcgcgc aggccggcga gcgtgtgcag cagcgtggtc ttgccgctgc
     5821 cgttggcgcc gagcaggccc cagcgctcgc cagcgcgcag cgcgaacccg agcccggtgc
     5881 agacgcggtg cgtgccgacc tcgacgctca gcccgcgcgc ttcgagcagc ggggcaggag
     5941 ccggggcagt tgcagttcct gcgtcagccg ccatgccggc cccggttcag cagccacagg
     6001 aaggccggca cgccgaccag cgccgtgacc gcgccgagcg gcagctgctg cggggcgatc
     6061 gcggtgcgcg ccagcgtgtc ggcgagcacc agc
//
LOCUS       DAOQJH010000050         6069 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_7368_length_6069_cov_3.770535, whole genome shotgun sequence.
ACCESSION   DAOQJH010000050 DAOQJH010000000
VERSION     DAOQJH010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 6069)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6069)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6069
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7368_length_6069_cov_3.770535"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="PLQ18_02070"
     CDS             complement(<1..295)
                     /locus_tag="PLQ18_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020483874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HNE00466.1"
                     /translation="MKITIFGTGYVGLVTGACLAEVGNHVLCVDVDETKIENLKCGII
                     PIYEPGLDEIVKRNLAAGTLEFTTEAAAGVAHGLFQFIAVGTPPDEDGSADLQH"
     gene            complement(498..2129)
                     /locus_tag="PLQ18_02075"
     CDS             complement(498..2129)
                     /locus_tag="PLQ18_02075"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020506388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HNE00467.1"
                     /translation="MDIRTVATTPFEGQKPGTSGLRKKVAVFRQPDYLENFVQSIFDT
                     VPALAGGTLVLGGDGRYHNRAAAQTILKMAAANGVARVLVGRGALLSTPAVSCVIRAR
                     AATGGIVLSASHNPGGPEADFGIKYNTGNGGPAPEAVTEAIHARTGRIGTYRIAEAAD
                     VDLDADGESSLGPMQVVVIDPVAEYAALMARLFDFDAIRVLFANGFRMRFDAMNAVTG
                     PYAHELLERRLGAPAGTVLRGTPLEDFGGEHPDPNLVHAHELVAALSGPDAPDFGAAS
                     DGDGDRNMILGRDFYVTPSDSLAVLAANATLIPGYRQGLAGVARSMPTSMAVDRVAEA
                     LGIECHETPTGWKFFGNLLDAGRITFCGEESFGTGSDHVREKDGLWAVLAWLNVLAVR
                     RESVADIVRAHWARYGRNFYTRHDYEGLDTRAADALMSALRTRLDSLPGTTVDGLAIE
                     AADDFAYTDPVDGSVSRAQGVRVRLAGGARIVYRLSGTGTEGATLRVYIERAEPDRDR
                     HGLDPQVALADLIAAAGRLAGIESHTGRSTADVIT"
     gene            complement(2166..2933)
                     /locus_tag="PLQ18_02080"
     CDS             complement(2166..2933)
                     /locus_tag="PLQ18_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020410593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1223 domain-containing protein"
                     /protein_id="HNE00468.1"
                     /translation="MRRRSMWIRLFAAAALLPAALVQAAQSFESPPQQVALLELFTSE
                     GCSSCPPADAWLSTLVRDPRLWQTLVPVAFHVDYWDGPGWQDRFATPAFSARQRRHVG
                     HGLVRQVYTPGFVVAGHEWRGWFGGETLAPAAPAAGVLHATLDGQRLDVRYTPHGSAD
                     GALQARVAVLGFGLGSEVRAGENAGRRLRHDFVVLGFDGFALHPDGSGHWTGQGTLPA
                     AKLPAPRHALAVWIERNGEPAPLQATGGWLALPAAGP"
     gene            3041..3685
                     /locus_tag="PLQ18_02085"
     CDS             3041..3685
                     /locus_tag="PLQ18_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018935084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF938 domain-containing protein"
                     /protein_id="HNE00469.1"
                     /translation="MNRIEPDSASLPWAPAADENKQAILTVLQRHLRSPASVLELGAG
                     TGQHAVHFATALPWLRWLASELPALLPDLAARLALAGLPNLPPPCPLDVLAGGEWPVP
                     GVDHAYTANTAHILPWRGVIALFAGLARSLRPDGLFFLYGPVLDGPASADSNLRFDAA
                     LRARDPLQGVRELADLLPLAKAHGFELLSIEAMPRDNVTLIWRLRPAPPAALPT"
     gene            complement(3709..4479)
                     /locus_tag="PLQ18_02090"
     CDS             complement(3709..4479)
                     /locus_tag="PLQ18_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006398315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HNE00470.1"
                     /translation="MQGEIRISREQAVLCLRVHRPGRRNALTNELYGALADAILGAQD
                     DPAVRVLLITGGEDCFTAGNDLGEFAAGPPADTDAPVFRFLSALVACEKPLVAAVCGV
                     AVGIGTTLLCHCDLVYAAQDARFQLPFVSLGLCPEAASSLLLPRLAGHARASEWLLLG
                     EPFGAAAAQAAGLVNEVLPAGEVLPRALARAQQLARQPPAAVRLTRQLLKAADRAAVE
                     RTLGIEAQAFLGRLASPEAAEAFAAFAEKRAPDFSRFD"
     gene            4563..5012
                     /locus_tag="PLQ18_02095"
     CDS             4563..5012
                     /locus_tag="PLQ18_02095"
                     /inference="COORDINATES: protein motif:HMM:NF023125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3293 domain-containing protein"
                     /protein_id="HNE00471.1"
                     /translation="MSPPPALALSGALLQAFLATRYTALTPFGPVEIRIGKRHPVLDR
                     ELARCGHDRWVYIGACNPRAQLLDDAHNRRRHLHFGRLLARLGRPGWSGHGLPADAAW
                     PPEPAWLVPGLDAALAHRLALRYGQLAYVCGGRETVAELRCSGLSSP"
     gene            complement(4999..5313)
                     /locus_tag="PLQ18_02100"
     CDS             complement(4999..5313)
                     /locus_tag="PLQ18_02100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE00472.1"
                     /translation="MLDPDVKHAYHPSEISGAGPVSGIHNNIICTGRCAIDFPSISVF
                     WFWPYLMLSLGSSVLLVGCLMLRVAWREHMPAHYRLLGGIAVLAGLAILLCAGWLGIR
                     GC"
     gene            5347..5982
                     /locus_tag="PLQ18_02105"
     CDS             5347..5982
                     /locus_tag="PLQ18_02105"
                     /inference="COORDINATES: protein motif:HMM:NF012920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HNE00473.1"
                     /translation="MGTTLGQRIREAREARGWSQLRLGESVGVSRAAVSQWESGESKG
                     LRPENLVAAARALAVNVEWLVTGVGAMRPDELAAGEGRSDYPLIDWSTAARWPDIGRN
                     AYRVGAEEWLPSPVPLGPAAFVLRVRGCSMAPRLLEGELVYIDPDRPPVHGGIVLVLP
                     GGATEPVLRELIVEGDRRVLRASNPDWPDPLRALSGLDTLIGSVAARLERF"
BASE COUNT          918 a         2133 c         2152 g          866 t
ORIGIN      
        1 cgtgctgcag gtcggccgaa ccgtcctcgt cgggcggcgt gccgacggcg atgaactgga
       61 acaagccgtg ggcgacgccg gctgcggctt cggtggtgaa ttcgagcgtg ccggcggcca
      121 gattgcgctt gacgatttcg tccagcccgg gttcgtaaat cggaatgatg ccgcacttga
      181 ggttttcgat cttggtttcg tcgacatcca cgcacagcac gtggttgccg acctcggcca
      241 gacaggcgcc cgtgacgagt ccgacgtagc cagtcccgaa aatcgtgatc ttcatgacag
      301 gtctcttgaa caggtagatt tcggcggtgg cggatacggg caacggtccg ccgcacgcaa
      361 gtcgcggcac cttacccgcc ggctcacggt actggcaagc cggatgccgc agtgcagcaa
      421 gggatttgcc gatccggcac catcggaaac atttcaaccc ggcggctgcg ggtcgtgatt
      481 cgacgtccgg cggacgctca ggtgatcaca tcggcagtac tgcgaccggt gtggctttcg
      541 atgcccgcca gccgcccggc ggcggcaatc aggtcggcca gtgccacctg cggatccagg
      601 ccgtggcggt cccgatccgg ctctgcacgc tcgatgtaga cgcgcagtgt cgcgccctcg
      661 gtgccggtgc cggaaagccg gtagacgatg cgcgcgcctc cggcgaggcg cacccgcacc
      721 ccctgtgcgc ggctgacgct gccatccacc ggatcggtgt aggcgaagtc gtccgccgct
      781 tcgatcgcaa ggccgtccac cgtcgtgccg ggcagcgagt cgaggcgggt ccgcagtgcg
      841 gacatcaggg catcggcggc ccgggtgtcc aggccttcgt agtcatggcg tgtatagaag
      901 ttgcggccgt agcgggccca gtgcgcccgc acgatgtcgg cgacgctttc ccggcgcacg
      961 gccagcacgt tcagccaggc cagcaccgcc cagagtccgt ccttctcgcg cacgtggtcc
     1021 gagccggtgc cgaaggattc ctcgccgcag aacgtgatgc ggccggcatc gagcaggttg
     1081 ccgaagaact tccagccggt cggggtctcg tggcactcga tgccgagcgc ttcggccacg
     1141 cggtcgacgg ccatcgaggt cggcatcgag cgggcaacgc cggcgagccc ctggcggtag
     1201 ccgggaatca gcgtggcatt ggcggcgagc acggccagcg agtcggatgg cgtcacgtag
     1261 aagtcgcgcc cgaggatcat gttgcgatcg ccgtcgccgt cggaggcggc gccgaaatcc
     1321 ggggcgtccg gaccgctcag ggcggcgacc agctcgtggg cgtgaacgag gttcgggtcc
     1381 gggtgctcgc cgccgaagtc ttcgagcggc gtgccgcgca gcacggtgcc ggccggtgcg
     1441 ccgagccggc gctccagcag ttcatgggcg tacggtccgg tcacggcgtt catcgcgtcg
     1501 aagcgcatcc ggaagccgtt ggcaaacagc acgcggatcg cgtcgaaatc gaacagccgc
     1561 gccatcagcg ccgcgtattc ggcgaccgga tcgatgacca cgacctgcat cgggccgagg
     1621 ctggattcgc cgtcggcgtc gagatccacg tcggcggcct cggcgatgcg gtaggttccg
     1681 atgcggccgg tacgggcatg gatcgcctcg gtgacggcct cgggcgccgg accaccgttg
     1741 ccggtgttgt acttgatgcc gaagtcggcc tcgggtccgc cggggttgtg cgaggccgac
     1801 agcacgatgc cgccggtcgc ggcgcgggcg cggatcacgc aggagacggc cggcgtcgac
     1861 agcagggcgc cgcgcccgac cagcacccgg gccacgccgt tggcggccgc catcttcagg
     1921 atcgtctgcg cggcggcgcg gttgtggtag cggccatcgc caccgagcac cagcgtgccg
     1981 ccggccagcg ccggcacggt atcgaagatc gactgcacga agttttcgag gtaatccggc
     2041 tggcggaaga cggcgacctt cttgcgcagt cccgaggtcc ccggcttctg accttcgaac
     2101 ggggtggtgg caacggttcg gatgtccatg cggcgaccgg tacggctggg gggaaggccg
     2161 acagtctacg gacccgccgc cggcagggcc agccagccgc cggtcgcctg cagcggcgcc
     2221 ggttcgccgt tgcgctcgat ccagaccgcc agtgcatggc gcggagccgg cagcttcgca
     2281 gccggcagcg tcccctgccc cgtccagtgc ccgctgccgt ccgggtgcag tgcgaagcca
     2341 tcgaagccga gcacgacgaa atcgtgcctg agcctgcgac cggcgttctc gccggcccgc
     2401 acctcgctgc cgagaccgaa accgagcacg gcgacgcgcg cctgcagggc accgtctgcc
     2461 gacccgtgcg gggtgtagcg cacatcgaga cgctgaccgt cgagcgtggc gtgcagcacg
     2521 ccggcggccg gagcggccgg ggccagggtc tcgcccccga accagccccg ccactcgtgc
     2581 ccggcgacga cgaagccggg ggtgtagacc tgacgcacca gaccgtgccc gacgtgccgg
     2641 cgctggcggg cgctgaaggc cggtgtcgcg aagcggtcct gccagccggg accatcccag
     2701 taatcaacgt ggaaggcgac cggcaccagg gtctgccaca gccgtggatc ccggaccagc
     2761 gtgctcagcc aggcatcggc cggcgggcag ctgctgcagc cttccgacgt gaacagttcg
     2821 agcagcgcga cctgctgcgg cggactttcg aagctctggg cggcctgcac cagggcggca
     2881 ggcagcaggg cggcagcggc gaacaggcga atccacatcg atcggcggcg catcacggat
     2941 atcctcgaca gcggaaccgc ccgcgggaaa cgggccgtct ccgttacgct gccggacgcc
     3001 ggccggatgc aggcgccgga ctcacctgaa accgtggacc gtgaaccgca tcgaacccga
     3061 ctccgcttcc ctgccctggg ccccggcggc cgatgagaac aagcaggcca tcctcacggt
     3121 gctgcaacgc catctgcgct cgccggcgag cgtgctcgaa ctcggcgccg gcaccggtca
     3181 gcacgccgtg catttcgcca ccgccctgcc ctggctgcgc tggctcgcga gtgagctgcc
     3241 ggccctgctg cccgatcttg ccgcccggct cgcgctcgcc gggctgccga acctgccgcc
     3301 gccctgcccg ctggatgtgc tggccggcgg cgaatggccg gtgcccggtg tcgatcacgc
     3361 ctacacggcc aataccgccc acatcctgcc gtggcgcggc gtcatcgccc tgttcgccgg
     3421 actggcgcgc tcgctgcgcc cggatggcct gttctttctg tacgggccgg tgctcgacgg
     3481 cccggcctcg gcggacagca acctgcgctt cgatgccgcg ctgcgcgccc gcgatccgct
     3541 gcagggcgtg cgcgaactcg ctgatctgct gcccctggcg aaggcgcacg gcttcgagct
     3601 tctgtcgatc gaagcgatgc cgcgcgacaa cgtgacgctg atctggcgct tgcggcccgc
     3661 gccgcctgcc gccctgccga catgaatcgc ctgcaggccg cgagcggatc agtcgaagcg
     3721 cgagaagtcc ggcgcgcgct tctcagcgaa cgccgcgaaa gcctccgcgg cctccgggga
     3781 ggccagccgg cccagaaagg cctgcgcctc gatgcccagc gtgcgctcga ccgccgcccg
     3841 gtcggcggcc ttcagcaact gccgggtcag gcgcacggcg gccggcggct ggcgggcgag
     3901 ctgctgcgcg cgggccagcg cgcggggcag gacttcgccg gcaggcagca cctcgttgac
     3961 cagccccgcc gcctgcgcgg ccgcggcccc gaacggctcg ccgagcagca gccactcgga
     4021 ggcacgcgcg tggccggcaa gccgcggcag cagcaggctg gaagccgctt ccggacagag
     4081 gccgaggctg acgaagggca gctggaagcg ggcatcctgc gcggcgtaga cgagatcaca
     4141 gtggcagagc agcgttgtgc cgatgccgac cgccacgccg cagacggccg caaccagcgg
     4201 cttctcgcag gcgaccaggg cgctcaggaa acggaacacc ggtgcatcgg tgtcggccgg
     4261 cggaccggcc gcgaattcgc cgagatcatt gccggccgtg aagcagtcct cgccgccggt
     4321 gatcagcagc acacgcacgg ccgggtcgtc ctgcgcgccg aggatcgcat cggcgagcgc
     4381 gccatacagt tcgttggtca gggcgttgcg ccgcccgggc cgatgcacgc gcaggcagag
     4441 cacggcctgt tcgcggctga tccggatttc accctgcatg ccgatgcccc cagtgatccg
     4501 ctaccggcac gaagctacgc cgagcccggg tccacggcca gtgccgggcc gggtgatcgg
     4561 gcatgagtcc gcccccggcc cttgcgctgt ccggggcgct gctgcaggcg tttctggcga
     4621 cccgctacac ggcgctgacg ccgttcggtc cggtcgagat ccgcatcggc aagcggcatc
     4681 cggtcctcga tcgcgagctg gcacggtgcg gccacgaccg ctgggtatac atcggtgcct
     4741 gcaatccgcg ggcgcagctg ctcgatgatg cgcacaaccg caggcggcac ctgcacttcg
     4801 gacgactgct cgcgcggctc gggcggccgg gctggagcgg ccacggcctg ccggccgatg
     4861 ccgcctggcc gccggagccg gcctggctgg tgccggggct ggatgcggca ctcgcacacc
     4921 ggcttgcact ccgctacggt cagcttgcat acgtctgcgg cggccgtgaa acggtggccg
     4981 aactgcgctg cagcggactc agcagcccct gatccccagc cagccggcgc aaagcaggat
     5041 cgccaggccg gccaggactg cgatcccgcc gagcaggcgg tagtgcgccg gcatgtgttc
     5101 acgccaggcc acccgcagca tcagacagcc gaccagcagc accgagctgc cgagcgacag
     5161 catcaggtaa ggccagaacc agaagacgga gatggatgga aagtcgatgg cacacctccc
     5221 tgtgcagatg atgttgttgt ggatgccgga gactggtccg gctccggaaa tttcgctagg
     5281 atggtaagca tgcttgacat ccggatcaag catgcctgcc cgggtacggc agtagcatgc
     5341 cgcaccatgg gaacaacact tggacaacgc atccgggaag cacgggaggc caggggatgg
     5401 tcacagctca ggctgggcga atccgtcggc gtcagccgtg cggccgtctc gcaatgggaa
     5461 agcggggaat cgaagggctt gcgccccgaa aacctcgtgg cggccgcacg tgcgctcgcc
     5521 gtcaacgtcg agtggctggt cacgggagtc ggcgccatgc gccccgatga actcgccgcc
     5581 ggcgaaggcc gcagcgatta cccgctgatc gactggagca ccgcagcacg ctggcccgac
     5641 atcggccgca acgcctaccg ggtcggcgcc gaggaatggc tgccgtcgcc ggtgccgctc
     5701 ggacccgccg cgttcgtgct gcgcgtgcgc ggctgcagca tggcgccccg cctgctcgaa
     5761 ggcgaactcg tctacatcga ccctgaccgg ccgccggtgc atggcggcat cgtgctggtg
     5821 ctgccgggcg gcgccaccga accggtgctg cgcgagctga tcgtcgaggg cgaccggcgg
     5881 gtgctgcgcg cctcgaaccc cgactggccc gatccgctgc gtgcgctgtc ggggctggat
     5941 acgctgatcg gcagcgtggc cgcccggctc gaacgcttct gatccctgcc ggtgatccct
     6001 gcctgcccgt ttgcgcgtcc acgtccgggt gttcccgccc tgcgccggcc tgcccccgca
     6061 aaaccggtg
//
LOCUS       DAOQJH010000051         6063 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_7379_length_6063_cov_4.316744, whole genome shotgun sequence.
ACCESSION   DAOQJH010000051 DAOQJH010000000
VERSION     DAOQJH010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 6063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6063
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7379_length_6063_cov_4.316744"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..615
                     /locus_tag="PLQ18_02110"
     CDS             <1..615
                     /locus_tag="PLQ18_02110"
                     /inference="COORDINATES: protein motif:HMM:NF014722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase SelO family protein"
                     /protein_id="HNE00474.1"
                     /translation="DDGRYAFDRQPSIAWWNLSAFGQTLTPLVPVERLRALLDGFSGQ
                     YAQAYAARMRAKLGLREARDEDEALLEDLLGLMAGCGADYTNTLRSLCDFRPGTGPQA
                     LLDTVADPAGFETWLQRYGERLRAEASDDETRAANMRRVNPKIVLRNWLAQRAIEQAE
                     NGDYAGIDRLLRLLQTPFDEHPEAPDCYGEPPDWARSLALSCSS"
     gene            complement(631..1200)
                     /locus_tag="PLQ18_02115"
     CDS             complement(631..1200)
                     /locus_tag="PLQ18_02115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE00475.1"
                     /translation="MPVSPGFTTCTTGLVAFWLSVLPCTPVLAEELALSLEPAQGPAL
                     VIGTVTLEADGDGERLTVAFDESRFEDHFLSMRPFRCLPAGEWLLCRLPYPYEHHRRI
                     RDSDLVDLEYALLFVIKPRSEYGIDLKDGLYWRLAREGRGFVGTPYAVDYTPLGVPPA
                     AGELRPLTHEQLWETEAQRLPFVRLRIGG"
     gene            complement(1283..2101)
                     /locus_tag="PLQ18_02120"
     CDS             complement(1283..2101)
                     /locus_tag="PLQ18_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005419027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNE00476.1"
                     /translation="MTTLAGRTLFVTGASRGIGRAIALRAARDGANIAVIGKTETPDP
                     RLPGTIHDTAAEIEAAGGRALALAVDVRDESAVLAAVAATLRRFGRLDVLVNNASAIS
                     LGGTLDTPMKRFDLMMAVNARATFLCAQACIPQLLRSDNPHILTLAPPPVTAARWYGA
                     HLAYTLAKGGMSWVTLGLAEEFRAQGLAVNALWPRTVIATSAIRLLPGVDPARCRKPE
                     IVADAAHAILTRPARSCTGRFFLDEEALAEAGITDFDAYAVTPGAALLPDIFLD"
     gene            complement(2127..3458)
                     /locus_tag="PLQ18_02125"
     CDS             complement(2127..3458)
                     /locus_tag="PLQ18_02125"
                     /inference="COORDINATES: protein motif:HMM:NF015316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNE00477.1"
                     /translation="MSASALPKPARTAGLSVLAALAATVAHGAGFQITESSVTGLGRA
                     FAGSGLVGDDLSAIAYNPAGLTLLDGTRIQGGATSISSDGEFRGRSTGTPSLRATGAP
                     IAGPVFSQDSSGGSSEDAGASAVVPNNYFVHALSPDLRVGFGLHSPFGLATDYGASWV
                     GRYHAQSSELQTVDLNPTLAWRLSPQLSLGFGLSYQLAKGRLSQAVFTGTPADGFAEF
                     KGDSDAFGWNLGAMYELAPGSRLGLSFRSKVTQDFDGDLRVRTPLGSRRFDAETSITL
                     PETVNLGYVQALDEQLSLSFGARWTNWSRFDELKLKSPGLAGGSKTFIEDWRDTWTLS
                     AGVDWRYSEAWTLRAGVAWDQTPVSSEEHRTARIPDADRWWLGFGASYRPTPKLTIDA
                     GFAHLFFEDADTRNVVTLDTGNPAIVIADSLSGSYSGSATLFGLQLQYRFD"
     gene            3935..5395
                     /locus_tag="PLQ18_02130"
     CDS             3935..5395
                     /locus_tag="PLQ18_02130"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNE00478.1"
                     /translation="MSFSDLSLDASLLRALTEAGYSEPTPIQAQAIPAALEGHDLIAS
                     AQTGTGKTAAFTLPALQRFAVASGDRRRAPRVLVLTPTRELAAQVMDAAHKYGRFLRF
                     TTVSLLGGMPYGPQLRALGGPLDLVVATPGRLIDHLDRDRIDLSKIEVLVLDEADRML
                     DLGFKEDVERIAEATPAGRQTLLFTATLDGAMARLAAELTRDAVRIAIEPTQTEQLNI
                     EQRLHVADDLAHKHRLLDHLLNDETMDRAIIFSATKRDADALAERLRDAGLPAAALHG
                     DMHQTLRSRTLARLRDGRVRLLVATDVAARGIDVQGITHVINFDLPKAAEDYVHRIGR
                     TGRAGRSGIAISLASYADVRQLERIERYTGKSIEEHVIEGLEPSRPLRRGRPGGGSGR
                     GRGGPRREGGGRGYGAPREGYRSSAPRGDRPAGERRSEGDARPAAPWRPREDDAARAP
                     REHTARPFAHRDDAARRRRSEGERRHDGAPRRYRED"
     gene            complement(5449..5676)
                     /locus_tag="PLQ18_02135"
     CDS             complement(5449..5676)
                     /locus_tag="PLQ18_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003233319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HNE00479.1"
                     /translation="MVLILYGTSECHLCEQAQEQVLAAAGRPAIEIDVAEDDGLYQRY
                     GLRIPVLRRQDTGAELDWPFDTGAVIALLGA"
BASE COUNT          922 a         2172 c         2087 g          882 t
ORIGIN      
        1 gacgacggcc gctacgcctt cgaccgccag ccgagcatcg cgtggtggaa tctgtccgcc
       61 ttcggtcaga cgctgacgcc gctggtgccg gtcgagcggc tgcgcgcgct gctcgatggc
      121 ttcagcggcc agtatgcgca ggcctatgcc gcgcgcatgc gcgcgaagct cggcctgcgc
      181 gaggcccgcg acgaggacga ggccctgctc gaagacctgc tcgggctgat ggccggctgc
      241 ggcgccgact acaccaacac cttgcgcagc ctctgcgatt tccggcccgg caccggcccg
      301 caggccctgc tcgataccgt cgccgatccg gccggcttcg agacctggct gcagcgctac
      361 ggcgagcgcc tgcgcgcgga agcctccgac gacgagaccc gcgccgccaa catgcggcgt
      421 gtcaatccga agatcgtgct gcgcaactgg ctcgcgcagc gcgcgatcga acaggccgag
      481 aacggcgact atgccgggat cgaccggctg ctgcgcctgc tgcagacgcc gttcgacgaa
      541 caccccgaag ccccggactg ctacggcgag ccgccggact gggcgcgctc gctggcgctc
      601 agctgttcgt cctgaaaaac accctgccgc tcagccgccg atgcgcaggc gcacgaacgg
      661 caggcgctgc gcctcggtct cccagagctg ctcgtgcgtc agcggtcgca gttcaccggc
      721 cgccggcggc acgccgagcg gcgtgtagtc gaccgcgtac ggcgtgccga caaagcccct
      781 gccctcgcgc gccagccgcc agtacagccc gtccttgagg tcgatcccgt attcgctgcg
      841 cggcttgatc acgaacagca gcgcgtattc gagatcgacc agatcgctgt cgcggatgcg
      901 ccggtggtgc tcgtacggat acggcagccg gcagagcagc cactcgccgg ccggcaggca
      961 gcgaaacggc cgcatcgaca ggaagtggtc ctcgaaccgc gactcgtcga aggcgaccgt
     1021 caggcgctcg ccgtcaccgt cggcttcgag cgtcaccgta ccgatcacga gcgccgggcc
     1081 ctgcgccggt tcgaggctca atgccagctc ttcggccagc accggggtgc agggcagcac
     1141 ggacagccag aacgccacga ggccggtcgt gcacgtggtg aagccggggg aaacgggcat
     1201 gggcggagtt ctccagcagc cggcggatgc agcggatacg ggtccggacg ctcgtgcgga
     1261 tgcccgggcc gttcgtgtcg ggtcagtcga ggaagatatc cggcagcagc gccgcgcccg
     1321 gcgtcacggc gtaggcgtcg aaatcggtga tgccggcctc ggccagggct tcctcgtcga
     1381 ggaagaagcg gccggtgcag ctgcgcgccg gccgggtcag gatcgcgtgc gcggcatcgg
     1441 cgacgatttc gggtttgcgg cagcgggccg gatcgacgcc gggcagcagc cggatcgccg
     1501 aggtcgcgat gacggtgcgc ggccacagcg cgttgaccgc cagcccctgc gcgcggaatt
     1561 cctcggcgag cccgagcgtc acccagctca tgccgccctt ggccagcgtg taggcaaggt
     1621 gcgccccgta ccagcgcgcg gcggtgaccg gcggcggcgc cagcgtcagg atgtgcggat
     1681 tgtccgagcg cagcagctgc gggatgcagg cctgcgcgca caggaaggtg gcgcgggcgt
     1741 tgacggccat catcaggtcg aagcgcttca tcggcgtgtc gagcgtgccg ccgaggctga
     1801 tcgccgaggc attgttgacc agcacgtcga gccggccgaa gcggcgcagc gtcgccgcca
     1861 ccgccgcaag cacggcggat tcgtcgcgca cgtcgacggc gagcgccagc gcccggccgc
     1921 cggcagcctc gatctcggcc gccgtgtcgt ggatggtgcc gggcaggcgc ggatcggggg
     1981 tttcggtctt gccgatcacg gcgatgttcg cgccgtcgcg ggcggcgcgc agggcgatcg
     2041 cgcggccgat gccacggctt gcaccggtca cgaacagcgt gcggcccgcg agcgtcgtca
     2101 tcggggtttc tcctcggtcc ggcccgtcag tcgaagcggt actgcagctg cagtccgaac
     2161 agcgtcgccg agcccgagta actgccgctc agcgaatcgg cgatgacgat ggccgggttg
     2221 ccggtatcca gcgtcacgac gttgcgcgtg tcggcatcct cgaagaacag gtgcgcgaag
     2281 ccggcgtcga tcgtcagctt cggcgtcggc cggtagctgg cgccgaagcc gagccaccag
     2341 cgatcggcgt ccgggatgcg tgcggtgcgg tgttcctcgg aactgaccgg cgtctggtcc
     2401 caggccacgc cggcgcgcag cgtccaggcc tcgctgtaac gccagtcgac gccggccgac
     2461 agcgtccagg tgtcgcgcca gtcctcgatg aaggtcttgc tgccgccggc gaggcccggg
     2521 gacttgagct tcagctcatc gaagcggctc cagttggtcc agcgcgcccc aaaggacagg
     2581 ctcagctgtt catcgagcgc ctgcacgtag ccgaggttga cggtctcggg cagcgtgatc
     2641 gaggtttcgg catcgaagcg acggctgccg agcggtgtgc gcacgcgcag gtcgccgtcg
     2701 aaatcctgcg tcaccttcga gcggaacgac aggccgaggc ggctgcccgg cgccagctcg
     2761 tacatcgcgc cgaggttcca gccgaaggca tcgctgtcgc ccttgaactc ggcaaagccg
     2821 tcagccggcg tgccggtgaa cacggcctgc gacagcctgc ccttggcgag ctggtagctg
     2881 aggccgaagc cgaggctcag ctgcggcgac agccgccagg ccagcgtcgg attcaggtcg
     2941 acggtctgca gttcggagga ctgcgcgtga tagcgcccga cccaggaagc gccgtaatcg
     3001 gttgccagtc cgaacggcga gtgcaggccg aagccgacgc gcaggtccgg cgacagcgca
     3061 tgcacgaagt agttgttcgg cacgacggcc gaggcgccgg catcctcgct gctgccgccg
     3121 ctgctgtcct gcgagaacac cgggccggcg atcggcgcac cggttgcacg cagcgacggc
     3181 gtgcccgtcg agcggccccg gaattcgccg tctgacgaaa tggaggtcgc gccgccctga
     3241 atgcgggtgc cgtcgagcag cgtcaggccg gcagggttgt aggcgatggc cgacaggtca
     3301 tcgcctacca ggccactgcc ggcaaaggcc cggccgagcc cggtgacgct ggattcggtg
     3361 atctggaaac cggcgccgtg ggcgacggtg gctgcaaggg ctgcaagaac ggacagcccg
     3421 gccgtacggg ccggtttcgg caaggcagat gcggacatcc tgatgttctc ccgtgttctc
     3481 ccggatccgg gggtgtcatg caggcggcct tcgagggccg ctctagtgcc tgacgtgccg
     3541 ggtaaaaatc cgtcccggcc gcagccggtc tagcgtagca gcgccggcag cgcgacctgc
     3601 ccctgttgcg atgaagcgac atccgtatcg aatacaaatc tcatcgtgaa catccgggga
     3661 atctgcctgc ggaaggcgcc tgtgtcgcag cgcagcagtg gcgcccggcc ggcatgcgcg
     3721 gtatagtgcg ccccacgtgc cggcgctctt gtccggcacg ccgtgtcatc gctccctgac
     3781 ctccccgacg ccggccttgc tgcgggcgta cgcgaaatcc cggttggcga cgatgtcgac
     3841 cgcgccggcg aaatttcggc cataccacgc attccacgtg acatcttccg gtccgtgccg
     3901 ccgtgcgccc ccgtattgca actccggtag cctaatgtcc ttttccgacc tgtctctcga
     3961 cgcgtccctg ctgcgtgccc tgaccgaagc cggctattcc gagccgaccc cgatccaggc
     4021 ccaggccatt cccgccgccc tcgaagggca tgacctcatc gcctccgcgc agaccggcac
     4081 cggcaagacc gctgccttca cgctgccggc gctgcagcgc tttgccgtgg cctccggtga
     4141 ccgccgccgt gcgccgcgcg tgctggtgct gaccccgacc cgcgagctcg ccgcgcaggt
     4201 catggatgcc gcgcacaagt acggccgctt cctgcgcttc acgaccgtca gcctgctcgg
     4261 cggcatgccc tacggcccgc agctgcgcgc gctgggcggc ccgctcgacc tggtcgtcgc
     4321 cacgcccggg cgcctgatcg atcacctcga ccgcgaccgc atcgacctgt cgaagatcga
     4381 ggtcctcgtg ctcgacgagg ccgaccgcat gctcgatctc ggcttcaagg aagacgtcga
     4441 gcgcatcgcc gaggccacgc cggccgggcg ccagacgctg ctgttcaccg cgaccctcga
     4501 cggtgcgatg gcgcgcctcg cggccgagct gacccgcgac gccgtgcgca tcgcgatcga
     4561 gccgacgcag accgagcagc tcaacatcga acagcgcctg cacgtcgccg atgacctcgc
     4621 gcacaagcac cgcctgctcg atcacctgct caacgacgag acgatggacc gcgcgatcat
     4681 cttctcggcg accaagcgtg acgccgacgc gctggccgag cgcctgcgcg atgccggcct
     4741 gccggccgcg gcgctgcacg gcgacatgca ccagacgctg cgctcgcgca cgctggcccg
     4801 gctgcgcgac ggtcgcgtac gcctgctggt cgccaccgat gtcgccgcgc gcggcatcga
     4861 cgtgcagggc atcacccacg tcatcaactt cgacctgccg aaggccgccg aggactacgt
     4921 gcaccgcatc ggccgcaccg gtcgtgccgg gcgcagcggc atcgcgatct cgctcgcgtc
     4981 gtacgccgac gtgcgccagc tcgaacgcat cgagcgctac accggcaagt cgatcgagga
     5041 gcacgtgatc gaagggctcg aaccgagccg gccgctgcgt cgcggccgtc cgggcggcgg
     5101 cagcggtcgc ggtcgcggcg gtccgcgtcg cgaaggcggc ggtcgtggct acggcgcacc
     5161 gcgcgagggc taccgcagca gcgccccgcg cggcgaccgg ccggccggcg aacgccgcag
     5221 cgaaggtgat gcccgtccgg cggccccctg gcgcccgcgc gaggacgatg ccgcccgtgc
     5281 tccgcgcgag cacacggccc gcccgttcgc gcatcgcgac gatgcggccc gccgccggcg
     5341 cagcgaaggc gaacgtcgtc acgacggcgc cccgcgccgc tatcgcgagg actgaagccc
     5401 ggcgggcacg gtcgtcacgc acacgtgctg atgaccgtgc catcgtgctc atgccccgag
     5461 cagtgcaatg acggcaccgg tgtcgaacgg ccagtcgagt tcggcaccgg tgtcctgtcg
     5521 gcgcagcacc gggatgcgca ggccataacg ctggtacagc ccgtcgtcct cggcgacatc
     5581 gatctcgatg gccggccgcc cggccgctgc cagcacctgc tcctgcgcct gctcgcacag
     5641 atgacattcc gaagttccgt acagaatcag caccatggcc tccccgaccg cgctcatcgc
     5701 cggcggccgt gcgtgaaatt tccgagccga cattgccgtg atccggatcg tgtggagccg
     5761 gatcggcatg ggctcctggc ggtttacggg gtgcgcggcc tgtgccaaca tgcctgccaa
     5821 cgtaaggaaa ccacgcgcag cgtaccgttc aagccattct ggcgcagctc acgggaagcc
     5881 aaaaccatgc aagggatgga cagtgtccgc ttcctgccgc cagccttctc cctgctggcg
     5941 acttccgcat catggctgtc gattcctttc attcccagcc tgcgacggac ccggccgtga
     6001 gccgcgtgca gcctctggtt gccgccgatt ccaccgctgc ggaatccggc ctcgacccgt
     6061 tgc
//
LOCUS       DAOQJH010000052         6059 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_7385_length_6059_cov_4.252332, whole genome shotgun sequence.
ACCESSION   DAOQJH010000052 DAOQJH010000000
VERSION     DAOQJH010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 6059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6059
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7385_length_6059_cov_4.252332"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1003)
                     /locus_tag="PLQ18_02140"
     CDS             complement(<1..1003)
                     /locus_tag="PLQ18_02140"
                     /inference="COORDINATES: protein motif:HMM:NF024750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase family protein"
                     /protein_id="HNE00480.1"
                     /translation="MPTTRIKFTKRALEAVPAAAAGKRETLLDTECPALAIRVTDKGV
                     KSFILQRRVNGKPTFVTLGRFPEMTVEQARKAATGAHVQINDGTDPNAVKRADRARKL
                     TLADVLDDYLKGRDLKPRTVAGYRRQVEVNLAEWKDKPLLSISRDMVERKHASLSEPR
                     SYVVNGRSVRRGSEAEANYAMRVLRALMTYAAGKYEDEDGAPILSDIPTRRLSATRAW
                     HRIERRQTVIKPTQLADLHAALWRVQPVRGPRYDDDARAYLELLLLTGLRKNEAAQLR
                     VADVDLAARTLTVPDTKNRRPHTLPLSDRLETLLRERIGAAGTDPATLLFPHVRDVRR
                     "
     gene            complement(1010..1402)
                     /locus_tag="PLQ18_02145"
     CDS             complement(1010..1402)
                     /locus_tag="PLQ18_02145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE00481.1"
                     /translation="MSDPRDCSGRDMMLSGCMTPMAAVIARLTIPQPEPMPEVVMVKP
                     ESEKHEARTGRASWIIGSGAEDGIRTRAACYRPSDFESDFRPVTHHHPSSLIVDISVT
                     WAHASITGHHSKPPKPYARRMPGVCPTW"
     gene            1803..1961
                     /locus_tag="PLQ18_02150"
     CDS             1803..1961
                     /locus_tag="PLQ18_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3309 family protein"
                     /protein_id="HNE00482.1"
                     /translation="MSLGTILLIVLILLLIGAIPAWPHSRDWGYYPSGGLGLVVVVVI
                     ILVLMGRI"
     gene            2421..5219
                     /gene="ppc"
                     /locus_tag="PLQ18_02155"
     CDS             2421..5219
                     /gene="ppc"
                     /locus_tag="PLQ18_02155"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019626396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HNE00483.1"
                     /translation="MPVTDDKGLRARVKLFGNLLGEVLRTQESGQVFDAVEALRKGFI
                     ELRTSEDPARRAQLMALIEALDPQTLTHVLRAFSSYFLLANIVEEDWAHQERRHRVAS
                     GEPLWRGSFDDTVRELVAAGVSAGQMQGLLDSLLFQPVFTAHPTEAKRRALLEAQRRI
                     FVMAKKLNDAGLGGYAREAVERQLLNQIQILWKTDEVRVHKPQVRDEIVNGLYYFRET
                     LFHALPHIYRNLERALATNFGEAAAAIRVPSFIRYGSWIGGDRDGNPFVTHVVTALAV
                     RLQARTVLREYVQRLEDLSHLLTHSSSLVRPSLAFAERLAADAPLVPEVYADEPHRFS
                     QEPYRRKLFLMRARLLRNLDRLNAAIDGHEAPVAAGYASERDFLEDLYAIRDSLHSHG
                     DGNLADAELKDLIRLAESFGFYLAQLDVRQESTRHTDAVTELFARAPNLPDYAALSED
                     ERLQVLGELLAHPGTPLLYAEDLSENTREILAVLRTVASLRDEISPNAFGAYVISMTH
                     AASHVMEVLFLASFAGLAGRRGDGSWFCDLRVSPLFETIADLAHIEPVLERLLDNPTY
                     RELLAVSGNVQEVMLGYSDSCKDGGILASAWNLYRAQKKIVTITARRDIRCKLFHGRG
                     GTVGRGGGPTHDAILAQPAGTVQGQIKFTEQGEVLSSKYSNPETAVYELTMAITGLIK
                     ASRCAVTECIPDEPQFLTVMEQLAKDGEQAYRQLTDHTPRFIDYFYEATPVTEIGLLN
                     IGSRPSHRKKADRSKYSVRAIPWVFGWGLSRQIIPGWYGLGAALSAYRSGDSGRLDTL
                     RRMYAEWPFFRSLLANAQMSLGKSEMGIAHEYAQLCLESGTADDIYGTIRSEHDETVA
                     QVLAVAGIEEILDENPRLKLSLARRDPYLDPINHIQICLLRRYRQAEGGGEAERALWL
                     NPLLRSINALAAGMRNTG"
     gene            complement(5268..6035)
                     /locus_tag="PLQ18_02160"
     CDS             complement(5268..6035)
                     /locus_tag="PLQ18_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018077560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent peptidase"
                     /protein_id="HNE00484.1"
                     /translation="MGWLRDWRRRRVLRQSEADAPTWQAACDAQAVLAGLPQADQQRL
                     RRLATLFLHEKTFEAAAGLTLTPPMRWRIAAVACLPVLNLGLDWYEGWSAIVVYPGEF
                     MARQDFTDEAGVVHQLRRPLIGEAWERGPVILSWADVLDSETATDGFNVVIHELAHKL
                     DLLNGEANGLPPLHRGMSVRAWADIFTRAYDDFCAHVDAGEDTAIDPYASDSPAEFFA
                     VLSEAFFETPAVLNATYPPVYAQLRAFYRQDPLLRLA"
BASE COUNT          957 a         1952 c         2050 g         1100 t
ORIGIN      
        1 gccggcgcac gtcgcgcaca tgcgggaaca gcagcgttgc cggatcggtg ccggcagccc
       61 cgatgcgttc gcgcagcagg gtttcgagcc ggtcggacag cggcaaggta tgcgggcggc
      121 ggttcttggt gtccggcacc gtgagggtgc gggcggcgag gtccacgtcg gcgacacgaa
      181 gctgcgcggc ctcgttcttg cgcaggccgg tgagcagcag cagttcgagg taggcgcggg
      241 catcgtcgtc gtaacgcgga ccgcgcaccg gctgcacgcg ccagagcgcc gcatgcaggt
      301 cggcaagctg cgtcggcttg atgacggtct ggcgccgctc gatgcggtgc catgcgcgcg
      361 tcgctgacag gcgccgggtc gggatgtcgg acaggatcgg tgcgccgtcc tcgtcctcgt
      421 acttgccggc ggcgtaggtc atcagcgccc gcagcacgcg catcgcatag ttggcttcgg
      481 cctcgctgcc gcggcgcacg ctgcgcccgt tcacgacgta gctgcgcggc tcgctcaggc
      541 tggcgtgctt gcgttcgacc atgtcgcggc tgatcgacag cagcggcttg tccttccact
      601 cggcgaggtt cacttcgact tgtcggcggt agccggcgac ggtgcgcggc ttcaggtcgc
      661 gccccttcag gtagtcgtcg agcacgtcgg ccagcgtcag cttgcgcgcc cggtcggcgc
      721 gcttgacggc gttcgggtcg gtgccgtcgt tgatctgcac atgcgcgccg gttgctgcct
      781 tgcgcgcctg ttcgacggtc atttccggaa agcggcccag cgttacgaaa gtcggcttcc
      841 cattcacccg ccgttgcagg atgaacgact tcaccccttt gtccgtaacg cgaattgcca
      901 gcgccgggca ttcggtgtcg agcagggttt cgcgcttgcc ggcagccgct gcaggcacgg
      961 cctccagagc acgcttcgtg aacttgattc gggtcgtcgg catcgggctt caccatgtcg
     1021 ggcatacgcc gggcatacgc cgggcatacg gtttgggtgg tttcgagtga tgcccggtga
     1081 tcgaagcatg cgcccaagtg actgaaatat caacgatcag tgatgacggg tgatgatggg
     1141 tgacggggcg aaaatcagac tcgaaatcgg atgggcggta acacgccgcc cgggttcgaa
     1201 tcccatcctc tgcgccagaa ccgatgatcc acgaagcccg cccggtgcgg gcttcgtgct
     1261 tttcggattc cggtttcacc atcacgactt caggcatcgg ttccggttgc gggattgtca
     1321 gtcgggcgat gacggccgcc atgggcgtca tgcatccgga aagcatcatg tcacgaccac
     1381 tgcagtcacg cggatcgctc attcattgcg ccagagtccg gcagggcagt ggatttcagt
     1441 ggatgccgga tcacgccgga agcctttcct gtaaggagtc gtggtcaccg tcctgatggt
     1501 cctgaggcaa cgtccggtgt ctggctgatc tgcttccggt tcctgctcag gcttgtggct
     1561 gtgcaaagac ttgcctgttc tgcgcaggaa cgcgcatcgg caccggtcgt actccggcgt
     1621 gtaagcatcg gagcgtgatc gacgattttt cgcctcgccg gaaccaagcc ggcgagcatg
     1681 ggtccatgtg ttcgttgcga tgattcaggc cgtcattcag atgcgtcgac gctttgcgga
     1741 tcggcgggag tgtctgtctg accgcgtaat tgccaacaac aagaccaaca ggagaacctc
     1801 ccatgtcgct cggtacgatt ctgctgatcg tgttgatcct gctgctgatc ggtgccattc
     1861 cagcctggcc gcacagtcgt gactggggct attacccgag tggcggtctg gggctggtcg
     1921 tggtggttgt gatcattctg gtattgatgg gcagaatatg acggacgtgc attcgccgaa
     1981 tttggcgtga tgcacgcaaa ccgcatggtc tgatccatct ccattcgttc aaccgcggcc
     2041 tgtccggtca ttgccggaca ggccgcggtt tttgtgtatg ccaatttttt gtccggaaat
     2101 cctgcgggtg tgcagacggg gctgcagggc ctgcaaatgc cttccctggc actgcagctc
     2161 gccgtgcacc gcaaaccggt ttcacgcgga atgtcaccgg gatcggggct cgccgtctgc
     2221 cgcccggtct ccaggcccgg aggcccggtt tgcagcgccg ccgcacacga ctccctggta
     2281 tttctgccga gcgcagttgc gcccgcccgc caggcccccg tcgtggacgt gccgcaccgc
     2341 accagactac gatcccccga cccgcaccgg ccgttcgcgc cgaccttccc cgcaagcgct
     2401 gctcccagca aggcccgtcc atgcccgtga ccgatgacaa ggggcttcgt gcccgcgtga
     2461 agctgttcgg caatctgctc ggcgaggtgc tgcgcacgca ggagtccggg caggtgttcg
     2521 atgccgtcga ggccttgcgc aagggcttca tcgagctgcg caccagtgag gacccggcgc
     2581 ggcgcgcgca gctgatggcg ctgatcgagg cgctcgaccc gcagacgctg acgcacgtgc
     2641 tgcgcgcgtt cagcagttac ttcctgctcg ccaacatcgt cgaggaggac tgggcgcatc
     2701 aggagcgtcg ccaccgcgtc gccagcggtg agccgctgtg gcgcggcagt ttcgatgaca
     2761 ccgtgcgcga actcgtcgcc gccggcgtca gcgccgggca gatgcagggc ctgctcgaca
     2821 gcctgctgtt ccagccggtg ttcaccgcac atccgaccga agccaagcgc cgggcgctgc
     2881 tggaagcgca gcggcgcatc ttcgtgatgg ccaagaagct caacgatgcc gggctcggcg
     2941 gctatgcgcg tgaggcggtc gaacgccagt tgctgaacca gatccagatc ctgtggaaaa
     3001 ccgacgaggt gcgcgtgcac aagccgcagg tgcgcgacga gatcgtcaac ggcctgtact
     3061 acttccgcga gacgctgttc cacgcgctgc cgcacattta ccgcaacctc gaacgggcgc
     3121 tggccacgaa tttcggcgaa gccgcggcgg cgatccgcgt gccgagtttc atccgctatg
     3181 gctcgtggat cggcggtgat cgcgacggaa atccctttgt cacgcacgtg gtcaccgcgc
     3241 tcgccgtgcg tttgcaggcg cgtacggtgc tgcgcgaata cgtgcagcgg ctcgaagacc
     3301 tttcacacct gctgacgcat tcgagttcgc tggtgcgccc gtcactggca tttgccgagc
     3361 ggctggcggc cgatgcgccg ctggtgccgg aggtctatgc cgacgagccg catcgcttca
     3421 gccaggagcc gtaccggcgc aagctgttcc tgatgcgcgc gcggctgctg cgcaatctcg
     3481 accggctgaa tgcagcgatc gacgggcatg aggcaccggt tgctgccggt tatgccagcg
     3541 agcgcgattt tctcgaagac ctgtacgcga tccgcgattc gctgcattcg cacggcgacg
     3601 gcaatctcgc cgatgcggag ctgaaggatc tgatccggct cgccgagagt ttcgggtttt
     3661 atctcgcgca actcgacgtg cgtcaggaat cgacgcgcca taccgatgcc gtgaccgaac
     3721 tgttcgcgcg cgcgccgaac ctgcccgatt acgcggcgct gtccgaggac gagcgcctgc
     3781 aggtgctcgg tgaactgctc gcgcatccgg gcacgccgct gctgtatgcc gaggatctct
     3841 ccgagaacac gcgcgagatt ctcgcggtat tgcggaccgt tgccagcctg cgcgacgaga
     3901 tcagcccgaa tgccttcggc gcctacgtga tctcgatgac gcacgcggcg agtcacgtca
     3961 tggaggtgct gttcctcgcg agcttcgccg gactcgccgg ccggcgcggc gatggcagct
     4021 ggttctgcga tctgcgcgtt tcgccgctgt tcgaaaccat cgccgatctc gcgcacatcg
     4081 agccggtgct cgaacgtctg ctcgacaatc cgacctaccg cgaactgctg gcggtgtcgg
     4141 gcaacgtgca ggaggtcatg ctcgggtatt cggattcgtg caaggacggc ggcatcctgg
     4201 cctcggcctg gaacctgtac cgcgcgcaga agaagatcgt cacgatcacg gcgcgccgcg
     4261 acatccgctg caagctgttc cacggccgcg gcggcacggt cggtcgcggc ggcggcccga
     4321 cgcacgatgc gatcctcgcg cagccggccg gcaccgtgca gggacagatc aagttcaccg
     4381 agcagggcga ggtgctgtcg tcgaagtatt cgaatccgga aaccgcggtc tacgagctga
     4441 cgatggcgat caccggcctg atcaaggcca gtcgctgcgc ggtgaccgaa tgcattccgg
     4501 acgaaccgca gttcctgacg gtcatggaac agctcgcaaa ggacggcgag caggcctacc
     4561 ggcagctgac cgaccacacg ccgcgtttca tcgattactt ctacgaggcg acaccggtca
     4621 ccgagatcgg gctgctgaac atcggttcgc ggccctcgca ccgcaagaag gccgatcgtt
     4681 ccaagtattc ggtgcgtgcg attccgtggg tgttcggctg ggggctgtcg cggcagatca
     4741 ttccgggctg gtacgggctc ggtgcggcgc tgtcggcgta tcgcagcggt gacagcggcc
     4801 ggctcgatac gctgaggcgc atgtatgccg aatggccgtt cttccgctcg ctgctcgcga
     4861 atgcgcagat gtcgctcggc aagtcggaaa tgggcatcgc gcacgagtac gcgcagctct
     4921 gtctggagtc cggcaccgcg gatgacattt acggcacgat ccgcagcgag catgacgaaa
     4981 ccgtcgcgca ggtgctggcg gttgccggca tcgaggaaat cctcgacgag aatccgcgtc
     5041 tgaagctgtc gctggcgcgg cgcgatccgt atctggaccc gatcaaccac atccagatct
     5101 gcctgctgcg ccgctaccgt caggccgaag gcggcggcga ggccgagcgg gcgctgtggc
     5161 tcaatccgct gctgcgctcg atcaacgcgc tggcggcggg catgcgcaac accggctgag
     5221 gcccggcagc gggcggcgtc cgtgtcgtcg cccgcggacc tctgcgctca cgccagccgc
     5281 agcagcggat cctgccggta gaaggcccgc agctgcgcgt agaccggcgg atacgtggcg
     5341 ttcagcaccg ccggcgtctc gaagaaggct tcgctcagca ccgcgaagaa ctcggcgggg
     5401 ctgtcgctgg cgtagggatc gatcgcggtg tcctcgccgg catcgacgtg cgcgcagaag
     5461 tcgtcgtagg cgcgcgtgaa gatgtcggcc cacgcgcgta ccgacatgcc gcgatgcagc
     5521 ggcggcaggc cgttggcctc gccgttgagc aggtcgagct tgtgcgcgag ttcgtggatc
     5581 acgacgttga agccgtcggt ggcggtttcg ctgtcgagca cgtcggccca ggacaggatc
     5641 accggcccgc gttcccaggc ctcgccgatc agcggccggc gcagctgatg cacgacgccg
     5701 gcctcgtcgg tgaagtcctg ccgggccatg aattcgccgg gatagacgac gatcgccgac
     5761 cagccctcgt accagtcgag gccgaggttc agcaccggca ggcaggcgac cgcggcgatg
     5821 cgccagcgca tcggcggcgt cagcgtcagg ccggcagcgg cctcgaaggt tttctcgtgc
     5881 aggaacagcg tcgcgagccg gcgcaggcgc tgctgatccg cctgcggcag gccggcgagg
     5941 actgcctgcg cgtcgcaggc cgcctgccag gtcggcgcat cggcttcgct ctggcggagc
     6001 acgcgccggc gacgccagtc ccgcagccag cccatcgtgc gtcagtcgcg tcgctgctg
//
LOCUS       DAOQJH010000053         6047 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_7405_length_6047_cov_3.772196, whole genome shotgun sequence.
ACCESSION   DAOQJH010000053 DAOQJH010000000
VERSION     DAOQJH010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 6047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6047
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7405_length_6047_cov_3.772196"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..1147
                     /locus_tag="PLQ18_02165"
     CDS             2..1147
                     /locus_tag="PLQ18_02165"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003138941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNE00485.1"
                     /translation="MTVRQFLRDYWQQKPLLVRKAIPGFKSPISPEELAGLACDEEVV
                     SRLVMERGGRTPWELRRGPFTEEDFLELPETHWTLLVSDVEKQAPDMAGLIEPFRFVP
                     DWRIDDLMISYAPEHGTVGPHVDDYDVFLLQGLGRRRWQISTQPVDPDNFLPDVELRI
                     LDEFEAEQEWVLEPGDLLYLPPKVAHHGIALGPCMTYSIGFRAPLHSDMIGRYVDFLM
                     EEADHELRYRDEGLAPQDNPGRIGADALDRIKDLIRSSLATGDDLIESWFGRYITEPK
                     PGYVAEPEDDPYTLAELRRHLKDGGQLERNPGSRFAYIDHGQDSVTLFVDGQEFALGP
                     ALATLARVLCRERILDSRRLQAALSGDDAWALLLDLLNEGFLVIYEG"
     gene            complement(1135..2022)
                     /locus_tag="PLQ18_02170"
     CDS             complement(1135..2022)
                     /locus_tag="PLQ18_02170"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HNE00486.1"
                     /translation="MPAVAAALGAGALWGLAFLAPLALADWSPLAISGGRYLAYALVA
                     LVALLAGPRRPTSAREWLAAWLLSLLGNVVYYLLFAYAVRAAGSYLPTLVIGALPVTL
                     AIVGQWREHRPWRVRHLPPLALIVAGLACVNLGESGTVRGEDYPAGVLAAVAALACWT
                     LFGAANSDYLKRHPALPAGHWNNLLGAGLLPVALLLLAVAPDTAARSPADWQHFWWIA
                     AGTGLTAGWAASGFWNLASRGLPVALAGQLIVCETLFASAYAALWHGALPPAATLAGA
                     ALLVAGVLLAIRAFAAQPS"
     gene            complement(2052..2900)
                     /gene="panC"
                     /locus_tag="PLQ18_02175"
     CDS             complement(2052..2900)
                     /gene="panC"
                     /locus_tag="PLQ18_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018717202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HNE00487.1"
                     /translation="MNRVNGIAELRAQVAAWKRAAERVALVPTMGNLHAGHIRLVEEA
                     RRHADRTVASIFVNPMQFGPNEDFASYPRTLEADADKLAAAGLDLLFAPPVEAIYPNG
                     HLGMTTVSVPTITGHLCGSARPGHFDGVSTVVTKLFNLVQPDLALFGEKDWQQLQVIR
                     RMVRDLDVPIGIIGVPTVREADGLAMSSRNGYLSAAERALAPTLYTVLDAAAARIRAG
                     ERNYAAITEAANATLLDAGFTPDYFEVRRADDLLPAGPADHDLRLLAAARLGRARLID
                     NLGVTL"
     gene            complement(2979..3800)
                     /gene="panB"
                     /locus_tag="PLQ18_02180"
     CDS             complement(2979..3800)
                     /gene="panB"
                     /locus_tag="PLQ18_02180"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011628313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HNE00488.1"
                     /translation="MYLGEPRTRPVTVSTLRKMKASGEKIAMLTAYDASFAAMMERAG
                     VDVILVGDSLGMVVQGRESTVPVTVDDIAYHTSLVTRGCRLPLVIADMPFMSYATVDS
                     ALANAGRLMQEGGAQMIKLEGGQNVVEIVRLLSANGVPVCAHLGLQPQSVHKLGGYKV
                     QGRDEATAQRLLDDALVLQEAGADLLVLECVPAELGAKLTAELAIPTIGIGAGLGCDG
                     QVLVCYDMLDLTAGMRPKFSKNFMIGQERGVHGALEAYVRAVKDGSFPAAEHTFT"
     gene            complement(3847..4395)
                     /gene="folK"
                     /locus_tag="PLQ18_02185"
     CDS             complement(3847..4395)
                     /gene="folK"
                     /locus_tag="PLQ18_02185"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012486069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HNE00489.1"
                     /translation="MNPDNTVTVTRAWIGLGSNLEAPRRQVERAFDELARLPRTHLAG
                     RSRLYRSDPVGPAGQPDYVNAVAALDTALEPLALLDALQAIERTHGRVRTLRWGPRTL
                     DLDLLLYGERVQACERLTLPHPRLHERAFVLYPLADLAPGLVLPGLGALSELLRQCPY
                     TGLEVLPEHVRPGGFAPTARSL"
     gene            complement(4392..5879)
                     /gene="pcnB"
                     /locus_tag="PLQ18_02190"
     CDS             complement(4392..5879)
                     /gene="pcnB"
                     /locus_tag="PLQ18_02190"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018953087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004652 - polynucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HNE00490.1"
                     /translation="MGAWPELALPASGAYYTNLYAPHKRLVQGSPPLEDHRPDHPLII
                     PRSEHVISRSKISPNALKVLYRLRAAGFRGCLVGGGVRDLLLGREPKDFDVATDAHPE
                     EVYRLFRNCRLIGRRFRLAHVQYGSEIIEVATFRAQTPADDEDGAGGSGGVVTDAEGR
                     ILSDNVYGTIEDDAWRRDFTVNALYYDVADFSVLDYVGGMEDLKAGVIRLIGDPEQRY
                     REDPVRMLRAIRFAAKLGMRLHADTEAPIHTLGHLLRDIPPARLFDEVLKLFMAGSGV
                     QTFELLRLHGLFRHLFPATDRCLSRELNGFPRTLLVRALASTDERVAADKSVTPAFLY
                     AALLWEPVRSRYRLLHDGAASPLEAMQRAADECIERQLGHTALPRRYALPMREIWQLQ
                     PRFEDLHPRRAARLAAHPRFRAAYDFLLLRAESGEPVSDLAGWWTRWIDGPEATVAAP
                     ADGDTDTDTDADADAPEAAAETAGHGPRRRRRRRTGRRSSPAPEA"
     gene            5872..5947
                     /locus_tag="PLQ18_02195"
     tRNA            5872..5947
                     /locus_tag="PLQ18_02195"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5906..5908,aa:Glu,seq:ctc)
BASE COUNT          923 a         2081 c         2197 g          846 t
ORIGIN      
        1 cctgacggtc cgccagttcc tgcgcgacta ctggcagcag aagccgctgc tggtgcgcaa
       61 ggccataccg ggattcaagt cgccgatctc gcccgaggaa ctcgccggac tcgcctgcga
      121 cgaggaagtc gtctcgcgcc tggtcatgga gcgtggcgga cgcacgccgt gggagctgcg
      181 ccgcggcccg ttcacggaag aagacttcct cgaactgccc gagacgcact ggacgctgct
      241 ggtgtccgac gtcgagaagc aggccccgga catggccggg ctgatcgagc cgtttcgctt
      301 cgtgccggac tggcgcatcg atgacctgat gatcagctac gcgcccgagc acggcacggt
      361 cggcccgcac gtcgatgact acgacgtgtt cctgctgcag ggcctcggca ggcggcgctg
      421 gcagatcagc acgcagccgg tcgatccgga caacttcctg cccgatgtcg agctgcggat
      481 cctggacgag ttcgaggccg agcaggaatg ggtgctcgaa cccggcgacc tgctgtatct
      541 gccgccgaag gtcgcgcacc acggcatcgc gctcgggccg tgcatgacct actcgatcgg
      601 tttccgcgcg ccgctgcact ccgacatgat cgggcgctac gtcgacttcc tgatggagga
      661 agccgaccac gaactgcgct accgcgacga gggcctcgcg ccgcaggaca acccggggcg
      721 catcggcgcg gacgcgctcg atcgcatcaa ggacctgatc cgcagctcgc tcgcgaccgg
      781 cgatgacctg atcgagagct ggttcgggcg ctacatcacc gaaccgaagc cgggctacgt
      841 cgccgagccc gaggacgatc cgtacacact cgccgaactg cgccgccacc tgaaggacgg
      901 cggccagctc gaacgcaatc cgggctcgcg cttcgcgtac atcgaccacg gccaggacag
      961 cgtcacgctg ttcgtcgacg gtcaggagtt cgcgctcggg ccggcgctcg cgacgctcgc
     1021 gcgcgtgctc tgccgcgaac gcatcctcga cagccgccgg ctgcaggcgg cgctaagcgg
     1081 tgacgatgcc tgggcgctgc tgctcgacct gctgaacgaa ggcttcctcg tcatctacga
     1141 gggctgagcg gcgaaggcgc ggatcgcgag cagcacgccg gccaccagca gcgcggcacc
     1201 ggcgagcgtc gctgccggcg gcagcgcgcc gtgccagagc gcggcatagg cactcgcgaa
     1261 caaggtctcg cagacgatca gctggccggc cagcgccacc ggcaggccgc ggctggcgag
     1321 gttccagaac ccgctcgccg cccagccggc ggtgagcccg gtgccggcgg cgatccacca
     1381 gaagtgctgc cagtccgcgg ggctgcgcgc ggccgtgtcc ggcgcgacgg ccagcagcag
     1441 cagcgcaacc ggcagcaggc cggcaccgag caggttgttc cagtgcccgg ccggcagcgc
     1501 cggatggcgc ttcaggtagt cgctgttggc ggcaccgaac agcgtccagc acgcaagcgc
     1561 cgcgacggct gcgagcacgc cggccggata gtcctcaccg cgcaccgtgc cgctctcgcc
     1621 gaggttcacg caggcgagcc cggcgacgat cagcgcgagc ggcggcaggt gccgcacccg
     1681 ccacggccgg tgctcgcgcc actggccgac gatcgcgagc gtcaccggca gggcgccgat
     1741 caccagcgtc ggcagatagc tgccggcggc acgcaccgca tacgcgaaca gcaggtagta
     1801 gacgacgttg ccgagcagcg acagcagcca ggctgccagc cactcgcgcg ccgacgtggg
     1861 ccggcgcgga ccggcgagca gcgccacgag cgcgaccagc gcataggcga gatagcgccc
     1921 gccgctgatc gcgagcggcg accagtcggc gagtgcaagc ggagcgagga aggccagccc
     1981 ccacagcgcg ccggccccga gtgccgcggc gacggccggc agcggcaggg ccggtgccgc
     2041 tgcgcccggg ctcagagcgt gacgccgagg ttgtcgatca gccgcgcacg gccgagccgg
     2101 gcggcggcga gcaggcgcag gtcatggtcc gcggggccgg ccggcagcag gtcatcggcg
     2161 cggcgcacct cgaagtagtc cggcgtgaag ccggcatcga gcagcgtcgc gttcgcagcc
     2221 tcggtgatcg ccgcgtagtt gcgctcgccg gcgcggatgc gcgcggcggc ggcatcgagc
     2281 accgtgtaca gcgtcggggc cagcgcccgc tcggcggctg acagatagcc attgcgcgaa
     2341 ctcatcgcca gcccgtcggc ctcgcgcacg gtcggcacgc cgatgatccc gatcggcacg
     2401 tcgaggtcgc ggaccatgcg ccggatcacc tgcagctgct gccagtcctt ctcgccgaac
     2461 agcgcgaggt cgggctgcac gaggttgaag agcttggtga cgacggtcga gacgccgtcg
     2521 aagtgcccgg gccgggcgct gccgcacagg tgcccggtga tcgtcggcac gctgaccgtg
     2581 gtcatgccga ggtggccgtt cgggtagatg gcctcgaccg gcggtgcaaa cagcagatcg
     2641 agcccggcgg cggcgagctt gtcggcgtcg gcctccagcg tgcgcggata gctcgcgaaa
     2701 tcctcgttcg gcccgaactg catcgggttg acgaagatgc tggcgaccgt gcgatcggcg
     2761 tggcggcggg cttcctcgac caggcggatg tggccggcgt gcaggttgcc catggtcggc
     2821 accagcgcga cgcgctctgc ggcgcgcttc cacgcggcga cctgcgcgcg cagctcggcg
     2881 atcccgttga cgcggttcat ggcggcgggg tcctcggcgg atgagattct gacaggagcc
     2941 cggccggcgg cggatgcacc ggccgggtga cggggggctc aggtgaaggt gtgttcggcg
     3001 gccgggaagc tgccgtcctt gaccgcgcgc acgtaggctt cgagcgcgcc gtgcacgccg
     3061 cgctcctgac cgatcatgaa gttcttcgag aacttcggac gcatgccggc ggtgaggtcg
     3121 agcatgtcgt agcagaccag cacctgaccg tcgcagccga gtccggcgcc gatgccgatg
     3181 gtcgggatcg cgagttcggc ggtcagcttc gcaccgagtt cggccggcac gcattcgagc
     3241 accagcagat cagccccggc ctcctgcagc accagcgcat cgtcgagcag gcgctgcgcg
     3301 gtggcctcgt cgcggccctg caccttgtag ccgccgagct tgtgcaccga ctgcggctgc
     3361 agcccgaggt gcgcacagac cggcacgccg ttggccgaga gcaggcgcac gatctcgacg
     3421 acgttctggc cgccttcgag cttgatcatc tgcgcgccgc cttcctgcat cagccggccg
     3481 gcgttggcga gcgcgctgtc gacagtcgcg tagctcatga acggcatgtc ggcgatcacc
     3541 agcggcagcc ggcagccgcg cgtgaccaga ctcgtgtgat aggcgatgtc atcgaccgtc
     3601 accggcacgg tgctctcgcg gccctgcacg accatgccga gcgagtcgcc gaccaggatc
     3661 acgtcgacgc cggcgcgttc catcatggcc gcgaagctcg cgtcatacgc ggtcagcatc
     3721 gcgatcttct cgccggaagc cttcatcttg cgcagcgtcg agaccgtgac cggccgcgtg
     3781 cgcggttcac cgaggtacat ggtttctggc cctcctgccg cgatccgggc agggcgatgg
     3841 ggtcggctac aggctgcggg cggtcggggc gaagccgccg gggcggacgt gttccggcag
     3901 cacttccaga ccggtgtacg gacactgccg cagcagctcg gacaaggccc cgagtcctgg
     3961 taagaccagt ccgggcgcca gatcggcgag aggatacagg acgaaggcgc gctcgtgcag
     4021 acgcgggtgc ggcagcgtca gccgttcgca ggcctgcacg cgctcgccgt agagcagcag
     4081 gtcgagatcg agcgtgcgcg ggccccagcg cagcgtgcgc acgcgcccgt gcgtgcgctc
     4141 gatcgcctgc agcgcatcga gcagcgccag cggttcgagc gcggtgtcga gcgcggcgac
     4201 ggcgttgacg tagtccggct gcccggccgg gccgaccgga tcggagcggt acagccgcga
     4261 gcggccggcc agatgcgtgc gcggcagccg cgcgagctcg tcgaaggcgc gctcgacctg
     4321 ccggcgcggc gcttcgaggt tgctgccgag gccgatccag gcgcgggtga cggtgacggt
     4381 gttgtcgggg ttcacgcttc gggtgccggg ctgctgcgtc tgccggtgcg ccggcggcgc
     4441 cggcgccgcg gcccgtgccc tgcggtttcc gcggcggcct ccggggcgtc ggcatcggcg
     4501 tcggtgtcgg tgtcggtgtc gccgtcggcg ggcgccgcga ccgtggcctc ggggccgtcg
     4561 atccagcgtg tccaccagcc ggcgagatcg ctgaccggct cgccggattc ggcccgcagc
     4621 agcaggaagt cgtaggcggc gcgaaagcgc gggtgcgcgg ccagccgcgc ggcgcggcgc
     4681 ggatggagat cctcgaagcg cggctgcagc tgccagatct cgcgcatcgg cagcgcgtag
     4741 cggcgcggca gcgcggtgtg gccgagctgg cgctcgatgc attcgtcggc ggcgcgctgc
     4801 atggcttcga gcggcgatgc ggcaccatcg tgcagcaggc ggtagcggct gcgcaccggc
     4861 tcccacagca gcgcggcgta caggaaggcc ggcgtcaccg acttgtcggc ggcgacgcgc
     4921 tcgtcggtgc tcgccagcgc ccgcaccagc agcgtgcgcg gaaagccgtt cagctcgcgc
     4981 gacaggcagc ggtcggtggc cgggaacagg tgacggaaca ggccgtgcag gcgcagcagc
     5041 tcgaaggtct gcacgccgct gccggccatg aacagcttca ggacttcatc gaagaggcgc
     5101 gccggcggga tgtcgcgcag caggtgaccg agcgtgtgga tcggcgcttc ggtgtcggca
     5161 tgcaggcgca tgccgagctt cgccgcgaag cggatcgcgc gcagcatgcg caccgggtcc
     5221 tcgcggtagc gctgctccgg gtcaccgatc agccggatca cgccggcctt caggtcctcc
     5281 atgccgccga cgtaatcgag caccgagaaa tcggcaacgt cgtagtacag cgcgttgacc
     5341 gtgaaatcac gccgccaggc gtcgtcctcg atcgtgccgt agacgttgtc gctgaggatg
     5401 cggccctcgg catcggtgac gaccccgccg ctgccgccgg cgccgtcctc gtcatcggcc
     5461 ggggtctgcg cgcggaaggt cgcgacctcg atgatctcgg agccgtactg cacgtgcgcg
     5521 agtcggaagc gccgcccgat cagccggcag ttgcggaaca gccggtagac ctcctcggga
     5581 tgggcatcgg tggcaacgtc gaaatccttg ggctcgcgtc cgagcagcag atcgcgcacg
     5641 cccccgccga ccagacagcc ccggaatccc gccgcgcgca ggcggtagag caccttcagc
     5701 gcgttcggac tgatcttcga gcgggatatg acgtgttcgg agcggggaat gatgagggga
     5761 tggtcggggc ggtgatcttc caaaggcggc gatccctgaa caagtcgctt gtgcggcgca
     5821 taaaggttcg tataatacgc cccgcttgcc ggcaacgcca gttcaggcca cgctcccatc
     5881 gtctagaggc ctaggacaca gccctctcaa ggctgcgacc ggggttcgaa tccccgtggg
     5941 agcgccagat tcaccttccg ccggcagtcc aagcaggtct ttttctgtcc cggctcccat
     6001 cgtctagagg cctaggacac agccctctca aggctgcgac cggggtt
//
LOCUS       DAOQJH010000054         5978 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_7534_length_5978_cov_3.430187, whole genome shotgun sequence.
ACCESSION   DAOQJH010000054 DAOQJH010000000
VERSION     DAOQJH010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 5978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5978
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7534_length_5978_cov_3.430187"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..559
                     /locus_tag="PLQ18_02200"
     CDS             <1..559
                     /locus_tag="PLQ18_02200"
                     /inference="COORDINATES: protein motif:HMM:NF012861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HNE00491.1"
                     /translation="VAFVQALRTVAGRSQAAGPALRLTLGGLACGVIGAGWPEVMGLG
                     YGTARAAIDGTLTALTLAPLLAAKFAATVLGLGLGLPGGLIGPSLVLGALAGGLSGHW
                     LPGADPGLCALLGMGAMMGATLNAPLAALVALLELTQDPRLILPAMLTIAAADLVSHR
                     VFGLPSVFVAQLQMRGIDWPPRERP"
     gene            621..1295
                     /locus_tag="PLQ18_02205"
     CDS             621..1295
                     /locus_tag="PLQ18_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018716420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HNE00492.1"
                     /translation="MTAVDQLALALGAGWASGLNLYAAVLALGYFNATGDLLLPPELQ
                     ILSDPLVMLAAGLMYCVEFFADKTPGVDSGWDALHTFLRIPAGAVLAAGAVGDVSPAL
                     QIAAGLVGGTLAAGTHFTKAGARVLINTSPEPFSNWAASVTEDVAVFAAIWTAVHHPA
                     LWLALLALSVALIVWLLPRLWRGIRRVLSSLARWFGFGSGRPPASPIPPAGPPPAPPR
                     LPPPAP"
     gene            1307..1810
                     /locus_tag="PLQ18_02210"
     CDS             1307..1810
                     /locus_tag="PLQ18_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020159980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNE00493.1"
                     /translation="MKLTDKLLPLAALAVVLGLVAFWLLPGGVNAAPAATFRTLDGRT
                     LTTADLKGKPAIVTFWATSCPGCMEEMPHLIELYRELQPKGLQIVGVAMSYDPPSNVM
                     ELVRQKQVPYPIALDTDEAIARAFGTIRVTPTSFLIAPDGRIVQQKIGAFDLPALKTQ
                     LKSMLGA"
     gene            1938..2375
                     /locus_tag="PLQ18_02215"
     CDS             1938..2375
                     /locus_tag="PLQ18_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018719319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HNE00494.1"
                     /translation="MSYLAIKAWHLIWMVCWFAGLFYLPRLYVYHAMLPPEDGAGHER
                     FVLMERKLFWGITTPAAVLTIGFGLWLLAAGGWALYSKMGWLHLKLALVALLVVYHVW
                     CYRYLRAFRELTNTRTHRWFRWFNEAPVIALIAVVLLASLKPF"
     gene            complement(2385..3212)
                     /locus_tag="PLQ18_02220"
     CDS             complement(2385..3212)
                     /locus_tag="PLQ18_02220"
                     /inference="COORDINATES: protein motif:HMM:NF015100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HNE00495.1"
                     /translation="MSVPVAPGPVPAPGRPGWRDPRLLQMVFQATLLAVGVGLRDFSL
                     AGAQAALAFAGGLGAQLFWLQVLGLRGVGLRSALITCFGIVLLLRADGLWQHPLAAAL
                     AISAKFVLRVRGRHLWNPANFGVVLALVLFPGAWASPGQWGQDLALALWFVALGGLVS
                     LRARSSDLAWTFLIAFLGLVALRVAWLGQSWAIWWHQLGNGALLLFAFFMISDPMTAP
                     DHPRARRLYACLVALVAALWQYGLFRPSGPLWALFLCAPLVPLFDRIWPGSRYRWGD"
     gene            complement(3209..4567)
                     /locus_tag="PLQ18_02225"
     CDS             complement(3209..4567)
                     /locus_tag="PLQ18_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009625061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2330 domain-containing protein"
                     /protein_id="HNE00496.1"
                     /translation="MIRPIAAALAGAALLACTQGAAAFCGFYVGKADASLFNEASQVI
                     VVRDGPRTVISMLNDYRGEPTDFALVVPVPQVLQKGQVHVGDKKIFERLDAYSAPRLV
                     EYFDPDPCRPPVVYAPAPTMAVQEGSAVRRRGADALGVTVESSFTVGEYDIVILSAKQ
                     SDGLETWLHDNGYRIPAGAAKALAPYIKQDMKFFVAKVNLKEQAKTGFTYLRPLQFAF
                     ESEKFMLPLRLGMINASGPQDLVAFMLTRKGRVETSNYRTVKLPANVDVPEFVKGEFP
                     AFYKALFGEQATREGLKAVFTEYFWDMAWCDPCAADPLSPAELRQAGVFWVGEGAQPP
                     AVNPRIAPMPPGGGAQPVMLTRLHLRYTPDTFPEDLMFQETADRQNFQARYVLRHAAE
                     VAPDACPQAKTYLEQLGQRREQEAQQLASLTGWNINDIRRKAKLDGPAPTAPKWWENL
                     WK"
     gene            complement(4635..5267)
                     /locus_tag="PLQ18_02230"
     CDS             complement(4635..5267)
                     /locus_tag="PLQ18_02230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE00497.1"
                     /translation="MSRFPRRASARLAVLLSAGLLPLSALHAQAGAGSADLPPDPLDA
                     RIEEARNAYGAGDYDTSIAALETVLAEVRLRKSNAYERWLPAPPKGWQASRPEAGAAP
                     QPAAGGIRAERRYTHGDEQLQLTITGESALLQGLGAVFDNPAALGSGPRIERLDGRRA
                     LYDPAGNALRVLVGNDAILVEARGNAAVSEATLQAFLRRVDFDTIEKSLR"
     gene            complement(5332..>5978)
                     /locus_tag="PLQ18_02235"
     CDS             complement(5332..>5978)
                     /locus_tag="PLQ18_02235"
                     /inference="COORDINATES: protein motif:HMM:NF024798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HNE00498.1"
                     /translation="TQFMPGTWLRHAVDGNGDGRIDLYGSLADAFASTAAYLAASGWP
                     AGQPWGWEVRLPAGFDWALAGEDRAQPLAAWAAAGVTRADGRPLAGGLNATLLVPTGR
                     QGPVFLLTDGFRAIMKYNASTSYALAVALLGDQLAGRPGLAADWPRSQPLLSVAETRE
                     LQERLAARGYDSGTPDGMLGTRTRTAVRGYQQRAGMVPDGWPTAELLARLRAEG"
BASE COUNT          815 a         2222 c         2093 g          848 t
ORIGIN      
        1 cgtggccttc gtgcaggcgc tgcgcacggt cgccggccgc tcgcaagcgg ccgggccggc
       61 gctgcgcctg acgctcggcg ggctggcctg cggtgtgatc ggcgccggct ggccggaggt
      121 catgggtctc ggctacggca cggcgcgcgc ggcgatcgac ggcacgctaa cggcgctgac
      181 gctggcgccg ctgctcgccg ccaagttcgc ggcgaccgtg ctcggtctcg gactcggcct
      241 gcccggcggg ctgatcggcc cgtcgctggt gctcggtgcg ctcgccggcg gcctgagcgg
      301 ccattggctg ccgggcgccg atccggggct gtgtgcgctg ctcggcatgg gcgcgatgat
      361 gggcgcgacg ctgaacgcac cgctggcagc cctggtcgca ctgctcgaac tgacgcagga
      421 cccgcggctg atcctgccgg cgatgctgac catcgccgcg gccgatctgg tctcgcaccg
      481 cgtgttcgga ctgccgtcgg tgttcgtcgc ccagctgcag atgcgcggca tcgactggcc
      541 gccgcgcgag cggccctgag gccgcacggc cggccgcccg ctttccccac cctccgcctg
      601 ccccgcagga ggtacgtcac atgaccgccg tcgatcagct cgccctcgcc ctcggcgccg
      661 gctgggcttc cggcctcaac ctgtatgccg cggtgctcgc gctcggctac ttcaacgcca
      721 ccggtgacct gctgctgccg ccggaactgc agatcctcag cgacccgctg gtgatgctcg
      781 cggccgggct catgtactgc gtcgagttct tcgccgacaa gacgcccggc gtcgacagcg
      841 gctgggacgc gctgcacacc ttcctccgca tcccggccgg tgccgtgctc gccgccggcg
      901 ccgtcggcga cgtgtcgccg gccctgcaga tcgccgccgg cctggtcggc ggcacgctcg
      961 ccgccggcac gcacttcacg aaggccggcg cgcgcgtgct gatcaacacc tcgccggagc
     1021 cgttcagcaa ctgggcggcc tcggtgaccg aggatgtcgc cgtgttcgcg gcgatctgga
     1081 ccgccgtgca tcacccggcg ctgtggcttg cgctgctcgc gctgtcggtg gcgctgatcg
     1141 tctggctgct gccgcggctc tggaggggta tccggcgcgt gctatcctcg ctcgcccgct
     1201 ggttcggctt cggcagcggc cggccaccgg catcgccgat cccgcccgcc ggcccgccac
     1261 cggctccccc gcggctgccg ccgcctgctc cctgatcgcc cgcgccatga aactcaccga
     1321 caagctgctg ccgctcgccg ccctcgccgt cgtcctcggc ctcgtggcgt tctggctgct
     1381 gccgggcggc gtcaacgccg ccccggccgc gaccttccgc acgctcgacg gccgcacgct
     1441 gaccacggcc gacctcaagg gcaagccggc gatcgtgacc ttctgggcca cgagctgccc
     1501 cggctgcatg gaggaaatgc cgcacctgat cgagctgtac cgcgaactgc agccgaaggg
     1561 cctgcagatc gtcggcgtcg cgatgagcta cgacccgccg agcaacgtca tggaactggt
     1621 gcgccagaag caggtgccct atccgatcgc gctcgacacc gacgaggcca tcgcccgcgc
     1681 cttcggcacg atccgcgtga cgccgacctc gttcctgatc gcgccggacg gacgcatcgt
     1741 gcagcagaag atcggtgcct tcgacctgcc ggcgctgaag acgcagctga agtcgatgct
     1801 cggcgcctga accccgcggc cctgcggccg gtccatccgc tgcgacacgg gcacccggtg
     1861 gtgcccgttt tgctttgtcc tgccgccacg gcaccccacg ctttcccgcc ccgccttcct
     1921 gcacggagac ccgaccgatg tcctatctcg cgatcaaggc ctggcacctg atctggatgg
     1981 tctgctggtt cgccggcctg ttctacctgc cgcggctgta cgtctatcac gcgatgctgc
     2041 cgcccgagga cggcgccggc cacgagcgct tcgtgctcat ggagcgcaag ctgttctggg
     2101 gcatcacgac accggcagcg gtgctgacga tcggcttcgg cctgtggctg ctcgcggccg
     2161 gcggctgggc gctgtattcg aagatgggct ggctgcacct gaagctcgcg ctggtcgcgc
     2221 tgctcgtcgt ctaccacgtc tggtgctacc gctacctgcg cgccttccgc gaactgacca
     2281 acacgcgcac gcaccggtgg ttccgctggt tcaacgaggc accggtcatc gcgctgatcg
     2341 cggtcgtgct gctcgcgagc ctgaagccgt tctgacgcga gacttcagtc gccccagcgg
     2401 tagcgcgacc ccggccagat ccggtcgaac agcggcacca gcggcgcgca caggaacagc
     2461 gcccacagcg ggccgctcgg ccggaacagc ccgtactgcc agagcgccgc caccagcgcg
     2521 accagacagg cgtacagccg gcgcgcgcgc ggatgatccg gcgccgtcat cgggtccgag
     2581 atcatgaaga acgcgaacag cagcagggcg ccgttgccga gctgatgcca ccagatcgcc
     2641 caggactggc cgagccacgc gacgcgcagc gccacgaggc cgaggaaggc gatcaggaag
     2701 gtccaggcca gatcgctgct gcgcgcgcgc aggctgacca gcccgccgag cgcgacgaac
     2761 cacagcgcca gcgccagatc ctgcccccac tgccccggcg aggcccaggc gcccgggaac
     2821 agcacgagcg cgagcacgac gccgaagttg gccgggttcc agaggtgtct gccgcgcacg
     2881 cgcagcacga acttggcgct gatggccagc gccgcggcca gcgggtgctg ccagaggccg
     2941 tcggcgcgca gcagcagcac gatgccgaag caggtgatca gcgccgagcg caggccgacc
     3001 ccgcgcagcc cgagcacctg cagccagaag agctgcgcgc cgagtccgcc ggcgaaggcg
     3061 agcgcggcct gcgccccggc cagcgagaaa tcgcgcaggc cgacgccgac cgccagcagc
     3121 gtcgcctgga aaaccatctg cagcagccgc ggatcgcgcc agccgggccg gccgggcgcg
     3181 ggcaccggcc ccggcgcgac cggcacgctc acttccacag gttctcccac cacttcgggg
     3241 ccgtcggggc cggcccatcg agcttcgcct tgcggcggat gtcattgatg ttccagccgg
     3301 tcaggctcgc gagctgctgc gcttcctgct cgcggcgctg gccgagctgt tcgaggtagg
     3361 tcttcgcctg cggacaggca tcgggcgcga cctcggcggc gtggcgcagc acgtagcgcg
     3421 cctggaagtt ctggcggtcg gcggtttcct ggaacatcag gtcttccggg aaggtgtccg
     3481 gcgtatagcg caggtgcagg cgcgtcagca tcaccggctg cgcgccgccg cccggcggca
     3541 tcggcgcgat gcgcggattc accgccggcg gctgggcgcc ctcgccgacc cagaacacgc
     3601 cggcctggcg cagctcggcc ggcgacagcg gatcggccgc acagggatcg caccaggcca
     3661 tgtcccagaa gtattcggtg aacaccgcct tcaggccctc gcgcgtcgcc tgctcgccga
     3721 acagcgcctt gtagaaggcc gggaactcgc ccttcacgaa ctccggcaca tcgacgttgg
     3781 ccggcagctt cacggtgcgg tagttgctgg tctcgacgcg gcccttgcgc gtcagcatga
     3841 acgcgaccag atcctgcggg ccgctggcgt tgatcatgcc gaggcgcagc ggcagcatga
     3901 atttttccga ttcgaacgcg aactgcagcg gccgcagata cgtgaagccg gtcttcgcct
     3961 gctccttcag gttcaccttg gcaacgaaga acttcatgtc ctgcttgata tacggcgcca
     4021 gcgccttcgc ggccccggcc ggaatccggt agccgttgtc gtgcagccag gtttcgaggc
     4081 cgtcggactg cttcgccgac aggatgacga tgtcgtactc gccgaccgtg aagctcgatt
     4141 cgaccgtgac gccgagcgca tcggccccgc gccggcggac cgccgagccc tcctgcacgg
     4201 ccatggtcgg ggccggcgcg tagaccaccg gcggccggca gggatcggga tcgaagtact
     4261 cgaccagcct cggcgccgaa tacgcatcga gccgctcgaa gatcttcttg tcgccgacgt
     4321 gcacctgccc cttctgcagc acctgcggca ccggcacgac cagcgcgaag tcggtcggct
     4381 cgcccctgta gtcgttcagc atcgagatca cggtgcgcgg accgtcgcgc acgacgatga
     4441 cctgcgaggc ctcgttgaac aggctggcgt cggccttgcc gacgtagaag ccgcagaagg
     4501 ccgcggcccc ctgcgtgcac gcgagcagcg ccgcaccggc gagtgcggcg gcgatcggac
     4561 ggatcatggg cgggactccg tgaaaatccg gggcggatgg cgcagcagcg tagagcccgc
     4621 gcgcgcgccc ggcctcagcg cagggatttt tcgatcgtgt cgaagtcgac gcggcgcagg
     4681 aaggcctgca gcgtcgcctc gctgacagcg gcattgccgc gcgcctcgac gaggatcgcg
     4741 tcattgccga ccagcacgcg cagcgcgttg ccggccgggt cgtacagcgc gcggcggcca
     4801 tcgagccttt cgatgcgcgg accgctgccg agcgcggccg ggttgtcgaa caccgcgccg
     4861 agcccctgca gcagcgccga ctcgccggtg atcgtcagct gcagctgctc gtcgccgtgc
     4921 gtgtagcggc gctcggcgcg gatgccaccg gcggccggct gcggcgcggc cccggcctcg
     4981 gggcggctcg cctgccagcc cttcggcggc gccggcagcc agcgctcgta ggcgttgctc
     5041 ttgcgcaggc gcacctcggc gagcacggtt tcgagcgccg cgatcgaggt gtcgtagtcc
     5101 ccggcgccgt aggcgttgcg cgcctcctcg atgcgcgcat cgagcgggtc cggcggcagg
     5161 tcggcgctac ccgccccggc ctgcgcatgc agcgccgaca gcggcagcag gccggcagac
     5221 agcagtacgg cgagccgggc ggaggcccgg cgcggaaatc tggacatgat cggctccggt
     5281 ggcggatgga aagcgccccc gccccggccc ggcatgggcg gcggacggct gtcagccttc
     5341 ggcgcgcagc cgcgccagca gttccgcggt cggccagccg tccggcacca tgccggcccg
     5401 ctgctggtag ccgcgcaccg ccgtgcgcgt gcgcgtgccg agcatgccgt cgggcgtgcc
     5461 gctgtcgtag ccgcgcgcgg ccagacgctc ctgcagctcg cgcgtctcgg ccaccgacag
     5521 cagcggctgc gagcgcggcc agtcggcggc gagccccggg cggccggcga gctggtcacc
     5581 gagcagcgcc acggccagcg cgtacgaggt cgaggcgttg tacttcatga tcgcgcggaa
     5641 gccgtcggtc agcaggaaca ccggcccctg ccggccggtc ggcaccagca gcgtcgcgtt
     5701 caggccgccg gcgagcggcc tgccgtcggc gcgcgtgacc ccggctgccg cccaggccgc
     5761 gagcggctgc gcacggtcct cgccggccag cgcccagtcg aagccggccg gcaggcgcac
     5821 ctcccagccc cagggctgac cggccggcca gccgctggcg gcgaggtagg ccgcggtcga
     5881 ggcgaaggca tcggcgaggc tgccgtagag atcgatgcgg ccgtcaccgt tgccatcgac
     5941 cgcgtgacgc agccaggtgc cgggcatgaa ctgcgtct
//
LOCUS       DAOQJH010000055         5914 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_7657_length_5914_cov_3.430961, whole genome shotgun sequence.
ACCESSION   DAOQJH010000055 DAOQJH010000000
VERSION     DAOQJH010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 5914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5914
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7657_length_5914_cov_3.430961"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(112..639)
                     /locus_tag="PLQ18_02240"
     CDS             complement(112..639)
                     /locus_tag="PLQ18_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017235165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HNE00499.1"
                     /translation="MHTSLVLTVIGPDRPGLVEAIAAVIARHGANWEESRMAQLGGQF
                     AGMLRVTAPEETQDALEDALAGLGSVGLKVTVARAAGAQAQVPKLLHLNLVGQDRPGI
                     VREIAEALARRGVSIESLETRCVSASMSGEMLFEAEAVLAVPPALAADELRTALEALA
                     NELMVDLDLAEPAGA"
     gene            complement(797..1171)
                     /locus_tag="PLQ18_02245"
     CDS             complement(797..1171)
                     /locus_tag="PLQ18_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017321925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2237 domain-containing protein"
                     /protein_id="HNE00500.1"
                     /translation="MTQRQHNVLGEPLAVCSVLPMTGFTRNGCCDAHPGDTGSHTVCV
                     RVTAEFLRFSRACGNDLSTPLPAWGFPGLRPGDRWCLCAARWQEALEAGVAPPVLLAA
                     THARALEHVRLDDLRRHALDPA"
     gene            complement(1232..2119)
                     /locus_tag="PLQ18_02250"
     CDS             complement(1232..2119)
                     /locus_tag="PLQ18_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HNE00501.1"
                     /translation="MIELLLRIGGIVFPVFAIAAVGALWGRRHRPDMSVVNRLNVEVF
                     APALVFWALADKPLDFDLYRDLAIGGIAVVLGSGLLLWPFVRLAGIDARTFIPPMMFN
                     NSGNMGIPLALFAFGETALQAAVVLFIIEMVLHFTVGLYILDHRTRPWRLLKMPMIQA
                     TIAGLACSAFGLTLPAPLANTIKLMGQVSIPLLLFALGVRLLDVNLRDWKLGTAGAFA
                     GPLAGVASLWLVLPWLALDEAQFPQLLIFAALPPAVLNVLVAEQYRQEPERVASIVLI
                     GNLGSLVVLPAALWFALPH"
     gene            complement(2160..3014)
                     /locus_tag="PLQ18_02255"
     CDS             complement(2160..3014)
                     /locus_tag="PLQ18_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNE00502.1"
                     /translation="MRTGSIRALSPRGFHTVRYVEWGEADNPEVLFCVHGLTRNGRDF
                     DRLARALAADYRVICPDVVGRGASDWLANKLDYGYPQYLTDLVALIARSGAEQVDWLG
                     TSMGGLIGLMLAAQPNHPIRRLVLNDVGPLIPKESLERIAAYVGAPQVFDTREDFELY
                     ARAIWAPFGLTSDGEWQDLLAHSGRRTGDGRWAFNYDPGIAEPFRQMAMADIDLWPLW
                     DRIRCPTLLLRGETSDVLPRSVAREMTTRGPKARLVEFPNCGHAPALMNAEQIATVRD
                     WLTTAETA"
     gene            3097..3834
                     /locus_tag="PLQ18_02260"
     CDS             3097..3834
                     /locus_tag="PLQ18_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012030916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNE00503.1"
                     /translation="MPALHHIEAGSGEALVLLHSGGMCHEEWKPQLPAFAKHHRTIAP
                     DLPGHGASPMRAAHLSIGDCGRGVLDTLDALGIERAHLVGSSMGGATALWLAVHHPAR
                     VAKLVLYRVSYHKNEHTHGGTREMADAAYWRGVGLAGWLSRIHQPQGGPDAWEQVITR
                     VSEALDPATSDHAHTIETLRELRCPTLVVCGDRDPLVPMDDVLAMYDHIPDCGLWLLP
                     HATHVTATNTWRAEAFATEFLRFLQRR"
     gene            3887..>4208
                     /locus_tag="PLQ18_02265"
     CDS             3887..>4208
                     /locus_tag="PLQ18_02265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE00504.1"
                     /translation="MSYRSRHCLALLVLVSGTALPAFAGPWLALCAKCLNPTVFRTQG
                     LDTAHAVAEARITREAAAGWCENWEPGRPVAACVAEQMAGDDAKRIWRAEADCTAGRI
                     TPVDG"
     assembly_gap    4209..4308
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4309..4830
                     /locus_tag="PLQ18_02270"
     CDS             <4309..4830
                     /locus_tag="PLQ18_02270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE00505.1"
                     /translation="IWRAEADCTAGRITPVDGETYTFAGVWRSDVGRGRTRWRDAQGR
                     IVGQDNASGGLGIAQQWEVLCPRTGTRPGSAGGRAPVPAPQPGAGTGEPPPANGGGHG
                     SGGFDAFFPAPAPAPEYRVGQAVEAKYLSDWLPARIEAVRPARRGGFEYDVRLANGKR
                     GAGVPPRLLRPAP"
     gene            complement(4834..>5914)
                     /locus_tag="PLQ18_02275"
     CDS             complement(4834..>5914)
                     /locus_tag="PLQ18_02275"
                     /inference="COORDINATES: protein motif:HMM:NF024257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding/OmpA
                     family protein"
                     /protein_id="HNE00506.1"
                     /translation="IELAAHGSGTAFTDLADGRADVGAASRPVRDEEIRKLAALGDFS
                     SPAAEHVIGLDGVAIIVHPANPLRSLSREQVAKLFAGEIRDWAELGGRPGPVHIYARD
                     ERSGTWDTFQSLVLGKAYPLSPEAQRFESSPELSDRVATDAGGIGFIGLPYVRMARAL
                     AIGDGNAVPSAPSPFTVATEDYPLSRRLFFYLAPTGDNRMARELVEFALENDGQQVVE
                     AHGFISQRVAPQRVALSPNAPKEYESLTRDALRLSVNFRFRPGSGLLDGKAQRDLRRV
                     TDYLAQAENRSRQVLLFGFTDARGSPEQNLRLSQQRAQEIARELQARGIQTRVVRGFG
                     PANPVAADDSPSGQERNRRVEIWVM"
BASE COUNT          890 a         2050 c         2082 g          792 t
ORIGIN      
        1 ccggcggtga gcgcacaccg gcaatctcct gatcttcagc cctgccctcc agagcggcct
       61 gacatgaatc gatcggcgat tcagaaactt gcgcagtccg gcaatccggg ctcaggcacc
      121 ggccggttcg gcgagatcga gatcgaccat cagttcgttg gccagggctt cgagtgccgt
      181 gcgcagttcg tcggcggcga gcgccggcgg cacggcgagc acggcctcgg cctcgaacag
      241 catctcgccg gacatcgagg cactgacgca gcgggtttcg agactctcga tcgacacgcc
      301 gcgccgggcc agcgcctcgg cgatctcgcg cacgatgccg gggcggtcct gaccgaccag
      361 gttcaggtgc agcagcttcg gcacctgcgc ctgggcgccg gcggcgcgcg cgacggtgac
      421 cttgaggccg acgctgccga gtccggcgag cgcatcctcg agggcatcct gcgtttcctc
      481 gggcgcggtc acgcgcagca tgccggcgaa ctggccgccg agctgcgcca tgcggctttc
      541 ttcccagttc gcgccgtggc gggcgatgac ggcggcgatc gcctcgacca gaccgggccg
      601 gtccgggccg atcaccgtga gcaccaggga cgtgtgcatg cgctgcgact ccgtgttggc
      661 aaaattggca atgttgtgag aactggccgg gctggcaaaa agaaccggaa ggatcttctg
      721 cgggatcggc agaacgccgg acgaggcccc gcacggggtg gcgcgctgac cgtacaggat
      781 gatgcctgcc ggcggctcag gccggatcga gtgcgtgccg gcgcaggtcg tcgaggcgca
      841 cgtgttcgag cgcgcgcgca tgcgtggcgg cgagcagcac cggcggcgcg acgccggctt
      901 cgagcgcctc ctgccagcgg gcggcgcaca gacaccagcg gtcgccgggc ctgaggcccg
      961 gaaaacccca ggccggcagc ggcgtcgaca ggtcgttgcc gcatgcgcgc gagaagcgca
     1021 ggaactcggc ggtcacgcgc acgcagacgg tgtggctgcc ggtgtcaccg ggatgggcat
     1081 cgcagcagcc gttgcgcgtg aagccggtca tcggcagcac cgaacagacg gccagtggtt
     1141 cgccgagcac gttgtgctgt cgctgggtca tggcggaagc ctcctgcggg gggacggggc
     1201 cggacggcag gactcgccgc cggactgcgt ttcagtgtgg caatgcaaac catagcgcgg
     1261 ccggcagcac cacgaggctg ccgaggttgc cgatcagcac gatcgaggcg acgcgctcgg
     1321 gctcctggcg gtactgctcg gccaccagca cgttcagcac cgccggcggc agcgccgcga
     1381 agatcagcag ctgcgggaac tgcgcctcgt cgagcgccag ccacggcagc accagccaca
     1441 ggctcgcgac cccggccagc ggtccggcga aggccccggc cgtgccgagc ttccagtcgc
     1501 gcaggttcac gtcgagcagg cgcacgccga gcgcgaacag cagcagcgga atcgacacct
     1561 ggcccatcag cttgatggtg ttggcgagcg gcgccggcag cgtcaggccg aaggcgctgc
     1621 aggcgagccc ggcgatcgtc gcctggatca tcggcatctt cagcagccgc cacggtcgcg
     1681 tgcggtgatc gaggatgtac aggccgaccg tgaagtgcag caccatttcg atgatgaaca
     1741 gcacgaccgc cgcctgcagc gccgtctcgc cgaacgcgaa cagcgccagc gggatgccca
     1801 tgttgccgct gttgttgaac atcatcgggg gaatgaaggt acgcgcgtcg atgccggcca
     1861 ggcgcacgaa cggccacagc agcagcccgg aaccgagcac gaccgcgatg ccgccgatgg
     1921 cgaggtcgcg atacagatca aagtcgagcg gcttgtcggc cagcgcccag aacaccagcg
     1981 ccggtgcgaa cacctcgaca ttgagccggt tgacgacgct catgtccggc cggtgccggc
     2041 gcccccacag cgcgccgacc gccgcgatcg cgaacaccgg aaagacgatg ccgccgatgc
     2101 gcagcagcaa ctcgatcaag cgcgcccctt cgcggacccc ggggccgggc tgccgggcat
     2161 caggccgtct ccgccgtcgt gagccagtcg cgcacggtgg cgatctgctc ggcattcatc
     2221 agcgccggcg cgtggccgca gttcgggaac tcgaccagcc gcgccttcgg gccgcgcgtc
     2281 gtcatctcgc gcgcgaccga gcgcggcagc acgtcggagg tctcgccgcg cagcagcagc
     2341 gtcgggcagc ggatgcggtc ccacagcggc cagaggtcga tgtcggccat cgccatctgc
     2401 cggaagggct cggcgatgcc ggggtcgtag ttgaaggccc agcgcccgtc cccggtgcgc
     2461 cggccggagt gcgcgagcag gtcctgccat tcgccgtcgc tggtcagtcc gaacggtgcc
     2521 cagatcgcgc gcgcgtacag ttcgaagtcc tcgcgcgtgt cgaacacctg cggcgcgccg
     2581 acgtaggcgg cgatgcgttc gagcgattcc ttcgggatca gcggaccgac gtcgttcagc
     2641 accagccggc ggatcgggtg attcggctgc gcggccagca tcaggccgat cagcccgccc
     2701 atcgaggtgc cgagccagtc gacctgttcg gcgccgctgc gcgcgatcag cgcgaccaga
     2761 tcggtcaggt actgcgggta gccgtagtcg agcttgttgg cgagccagtc gctggcaccg
     2821 cgcccgacca cgtccggaca gatcacgcgg taatcggccg cgagcgcacg cgccagacgg
     2881 tcgaaatcgc ggccgttgcg cgtcaggccg tgcacgcaga acagcacttc ggggttgtcg
     2941 gcctcgcccc actcgacgta acgcacggta tggaagccgc gcgggctcag ggcccggatc
     3001 gatccggtac gcatggcggg tgatcctcag gatggaaagt cgtctgcctg cagagcatac
     3061 aatgcccgtc cttgcggcga cgcggagacg ctaacgatgc cagccctgca tcacatcgaa
     3121 gccggcagcg gcgaggcact cgtgctgctg cacagcggcg gcatgtgcca cgaggaatgg
     3181 aagccgcagc tgccggcctt cgcgaagcac caccgcacca tcgcccccga cctgcccggt
     3241 cacggcgcga gcccgatgcg cgccgcgcac ctgtcgatcg gcgactgcgg ccgcggcgtg
     3301 ctcgacacgc tcgatgcgct cggcatcgag cgcgcgcatc tggtcggctc gtcgatgggc
     3361 ggtgcgaccg cgctctggct ggccgtgcat cacccggcgc gggtcgcgaa gctggtgctg
     3421 taccgcgtca gctaccacaa gaacgagcac acgcacggcg gcacgcgcga gatggccgat
     3481 gccgcctact ggcgcggcgt cggcctcgcc ggctggctgt cgcgcatcca ccagccgcag
     3541 ggcgggccgg atgcctggga gcaggtgatc acgcgcgtct cggaggccct cgacccggcc
     3601 acctccgatc acgcccacac gatcgagacg ctgcgcgagc tgcgctgccc gacgctggtg
     3661 gtctgcggcg accgcgatcc gctggtgccg atggatgacg tgctggcgat gtacgaccac
     3721 atcccggact gcgggctgtg gctgctgccg cacgcgacgc acgtgaccgc gaccaacacc
     3781 tggcgcgccg aggccttcgc gaccgagttc ctgcgcttcc tgcagcggcg ctgatcgtcc
     3841 gatccggcag ggatgcgcca ccgctccccg ggagggccac tgccgcatgt cataccgaag
     3901 ccgccattgc ctcgccctgc tcgtcctcgt ttccggcacg gccctgccgg ccttcgccgg
     3961 accatggctg gcgctctgcg cgaagtgcct gaatccgacc gtgttccgca cgcaggggct
     4021 cgataccgca cacgccgtcg ccgaggcgcg catcacgcgc gaggccgccg ccggctggtg
     4081 cgaaaactgg gagcccggcc ggccggtcgc cgcctgcgtg gccgaacaga tggccggcga
     4141 cgatgcgaag cgcatctggc gtgccgaggc cgactgcacg gccgggcgca tcacgccggt
     4201 cgatggcgnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnat ctggcgtgcc
     4321 gaggccgact gcacggccgg gcgcatcacg ccggtcgatg gcgagaccta caccttcgcc
     4381 ggcgtctggc gcagcgatgt cgggcgcggc cgcacgcgct ggcgcgacgc gcagggccgc
     4441 atcgtcggtc aggacaacgc cagcggcgga ctcggcattg cccagcagtg ggaggtgctc
     4501 tgcccgcgca ccggcaccag gccgggttcg gccggcggcc gtgcgccggt tccggcaccg
     4561 cagcccgggg ccggaaccgg cgaaccgccg ccggccaacg gcggcggcca cggaagcggc
     4621 ggcttcgatg cgtttttccc ggcgccggca ccggcgcccg agtaccgcgt cggtcaggcg
     4681 gtcgaagcga aatacctcag cgactggctg ccggcgcgca tcgaggccgt gcgtccggcc
     4741 cggcgcggcg gcttcgaata cgacgtgcgc ctcgccaacg gcaagcgcgg cgccggtgta
     4801 ccgccgcgcc tgctgcgccc ggcgccctga gcactacatc acccagatct cgacccggcg
     4861 gttgcgctcc tgtccgctcg gcgaatcgtc ggccgcgacc gggttggccg gtccgaagcc
     4921 gcgcacgacc cgggtctgga tgccgcgggc ctgcagttcg cgggcaatct cctgcgcgcg
     4981 ctgctgcgac agcctgaggt tctgctccgg actgccacgg gcatcggtga agccgaacag
     5041 cagcacctgg cggctgcggt tctcggcctg cgcgaggtag tccgtgaccc ggcgcaggtc
     5101 gcgctgcgcc ttgccgtcga gcagtccgct gccggggcgg aagcggaagt tcaccgacag
     5161 gcgcagcgca tcgcgggtca gtgactcgta ctccttcggg gcgttcggcg acagcgcgac
     5221 gcgctgcggg gcgacgcgct gcgagatgaa gccgtgggcc tcgaccacct gctgaccgtc
     5281 gttctcgagc gcgaactcga ccagttcgcg ggccatgcgg ttgtcgccgg tcggcgccag
     5341 atagaagaac aggcggcggg acagcgggta atcctcggtc gcgaccgtga acggactcgg
     5401 cgcgctcggc acggcattgc catcgccgat cgccagcgcc cgcgccatgc gcacgtaggg
     5461 cagaccgatg aagccgatgc cgccggcgtc ggtcgcgacc cgatccgaca gctcggggct
     5521 ggattcgaag cgctgtgcct cgggactgag cgggtaggcc ttgccgagca ccagcgactg
     5581 gaaggtgtcc caggtgccgg aacgctcgtc ccgcgcatag atgtgcaccg gacccggcct
     5641 gccgccgagt tcggcccagt cgcggatctc gccggcgaag agcttcgcga cctgctcgcg
     5701 gctcagcgag cgcagcggat tggccggatg gacgatgatc gcgacgccgt cgaggccgat
     5761 gacgtgctcg gccgccggcg acgagaaatc cccgagcgcg gcgagcttcc ggatttcctc
     5821 gtcccggacc ggccgcgatg cggcaccgac gtcggcccgg ccatcggcga ggtcggtgaa
     5881 ggccgtgccg ctgccgtgcg cggccagttc gatc
//
LOCUS       DAOQJH010000056         5872 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_7735_length_5872_cov_3.575382, whole genome shotgun sequence.
ACCESSION   DAOQJH010000056 DAOQJH010000000
VERSION     DAOQJH010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 5872)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5872)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5872
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7735_length_5872_cov_3.575382"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..996)
                     /locus_tag="PLQ18_02280"
     CDS             complement(<1..996)
                     /locus_tag="PLQ18_02280"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNE00507.1"
                     /translation="MNERFLMTRRELLMAGGALALGALLPAGRARAEAGKLVAATYPG
                     TWSEAHRNVLKPAFQKRVDAGVTQSIILGTDQVARLAAAKGQTPPFDVAIFDPPQVLD
                     AVRQGLIAEYPAAQSPHYAELLPVYQDRWGPKVSMQVIGLAYNPERIRTPPKRWDDLW
                     DPQYRGRVGMTALNSQLGLAFLAELNRLRGGSSSDFEPAFRALRELLPALGAVAANLG
                     AFATLWQQEQIDIAPYNFNFVQTLKAKGVPVEFVVPETGAVAWHTSLHVVAGTTVPEL
                     AVQYIDTHLDPLVQAAMMQPPYDVIPTNGKVALAGAVTQSVAKTQDALAALRGVDW"
     gene            complement(1146..2237)
                     /locus_tag="PLQ18_02285"
     CDS             complement(1146..2237)
                     /locus_tag="PLQ18_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020094700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNE00508.1"
                     /translation="MSGQRGHELQLEAITQRFHDFVAVRDINLRIGAGELVALLGPSG
                     CGKSTLLRIISGFMRQTEGRVLFDGEPVDHLSPSRRGVGIVFQNYALFPHLSIRQNIA
                     YGLEVQGVAKAKRDARVDEMLALVHMSEFAGRFPRQLSGGQQQRVALARCLAVDPKIL
                     LLDEPFGALDKNLRLDMQIEVKRLQREYGITTVLVTHDQEEALSMADRVAVMSRGRVE
                     QIASPTDIYDRPATLFVNQFVGSTNVLDGTVSTADADGSPVVALAGGRRIRVASDAPL
                     TAGSAVRVSIRPEHLHLHAEAPGADALPGAVQTALPLGALVVYDLALEGGAALKVSVP
                     RQRGEALLPAGAQVWAAPQPSAGYRAFPA"
     gene            complement(2243..3130)
                     /gene="uraD"
                     /locus_tag="PLQ18_02290"
     CDS             complement(2243..3130)
                     /gene="uraD"
                     /locus_tag="PLQ18_02290"
                     /EC_number="4.1.1.97"
                     /inference="COORDINATES: protein motif:HMM:NF020907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline
                     decarboxylase"
                     /protein_id="HNE00509.1"
                     /translation="MDIPIPDRDAAAQLEALNACPEDAFVAALGEVVEHSPWVVRRVA
                     ALRPFASVWALHAALAGVLRASDADTRTALLQRHPELAGREAGEGTLTADSNREQSRL
                     GLDRLSAAEHARLRALNTAYRERFGFPFITAVRLHADLASIFAEGERRLANAAATEAG
                     VALTQVAEVVRGRLAQRFGIAPGWLSTHVLDAVAGLPAAGVALTLSVQHAGGWRRLGG
                     FETDAEGRCPLLLCDDAMRAGTYQIEFRIGDYFRSRGHDGGGFLDTVPVRFVVSDAAL
                     HYHVPLLCTPASYTTYRGS"
     gene            3244..4191
                     /locus_tag="PLQ18_02295"
     CDS             3244..4191
                     /locus_tag="PLQ18_02295"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNE00510.1"
                     /translation="MPQSLLPKMLRYAEVVARAGSVQAAARVLNLSASAIDRQILLLE
                     DLLGAPLFERQPDGMRLSAAGELVVTLAKRWQHDASRLLTDIRQLQGINQGSLRLAAM
                     DSHANGLMPVFLERLHREHPRIRLEIDIVSPDQAEAALIAGTTDLAIAFNLKAGRDLH
                     LVMQAELPLGCVVAPGHPLAGQPDTSLKAVSGYPIVSQNRSLTIRRYLESRHGWLFAE
                     REAPVQTNSLQLVKHLAAAGSHVALTSELDAAPEILAGRLCFIPLTDRNVQPQTVAIA
                     ISARRPLPRIGRLVADLLAEELRGCLEAVRQRRAAGSGG"
     gene            complement(4269..4359)
                     /locus_tag="PLQ18_02300"
     tRNA            complement(4269..4359)
                     /locus_tag="PLQ18_02300"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4323..4325),aa:Ser,seq:gga)
     gene            complement(4466..>5872)
                     /gene="pnp"
                     /locus_tag="PLQ18_02305"
     CDS             complement(4466..>5872)
                     /gene="pnp"
                     /locus_tag="PLQ18_02305"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018138260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HNE00511.1"
                     /translation="GKPAWDWSAPAADTALDAAVRSAAFGSLVEAYQIAVKQDRQNRV
                     REIRGSVVEQLAGEGAAYTPEAVKTAYSNLEYRVVRDRILDGNPRIDGRDTKTVRPIT
                     VRAGVLPRTHGSALFTRGETQALVVATLGTTRDSQIIDALEGEYRELFMLHYNFPPFS
                     VGETGRVGSPKRREIGHGRLAKRGVAAVMPDMEKFPYVVRVVSEITESNGSSSMASVC
                     GSSLALMDAGVPLKAPVAGIAMGLIKEGERFAVLSDILGDEDHLGDMDFKVAGTTQGV
                     TALQMDIKIDGITKEIMQVALAQAREGRLHILGCMNAVLDRPRDDISSFAPRIVTIKI
                     NPDKIRDVIGKGGAVIRQLTEETGTTIDIDDDGTVKIAAVDKSASDEARRRIEMITAD
                     IEVGRIYEGKVVRIMDFGAFVSLLPGRDGMVHISQICEERIQSVTDKLAVGDSVRVKV
                     MEIDKQGRVRLTMRQVDA"
BASE COUNT          862 a         2060 c         2062 g          888 t
ORIGIN      
        1 ccagtcgacg ccgcgcagcg ccgcgagcgc gtcctgcgtc ttcgcgaccg actgcgtcac
       61 ggcgccggcg agcgccacct tgccgttggt cgggatcacg tcgtacggcg gctgcatcat
      121 cgccgcctgc acgagcggat cgaggtgggt gtcgatgtac tgcacggcca gctccggcac
      181 cgtcgtgccg gcgaccacgt gcaggctcgt gtgccaggcg accgcgccgg tctcgggcac
      241 gacgaactcc accggcacgc ccttggcctt cagcgtctgc acgaagttga agttgtacgg
      301 cgcgatgtcg atctgctcct gctgccacag cgtcgcgaac gcgccgaggt tcgccgccac
      361 cgcgccgagc gcgggcagca gctcgcgcaa tgccctgaac gccggttcga agtcgctgct
      421 gctgccaccg cgcagccggt tcagctcggc gaggaaggcc agccccagct ggctgttgag
      481 cgccgtcatg ccgacgcggc ccctgtactg cggatcccag agatcgtccc agcgcttcgg
      541 cggggttctg atccgctccg ggttgtaggc aaggccgatg acctgcatcg agaccttcgg
      601 cccccagcgg tcctgataca ccggcagcag ctcggcgtag tgcggcgact gcgcggccgg
      661 gtattcggcg atcaggccct ggcgcacggc atcgagcacc tgcggcggat cgaagatcgc
      721 cacgtcgaac ggcggcgtct ggcccttcgc ggccgcgagg cgcgcgacct ggtcggtgcc
      781 gaggatgatc gactgcgtga cgcccgcatc gacgcgcttc tggaaggccg gcttcagcac
      841 attgcggtgc gcctcgctcc aggtgccggg ataggtcgcc gccaccagct tgccggcctc
      901 ggcacgcgcc cgccccgccg gcagcagcgc gcccagcgcc agggcaccgc cggccatcag
      961 cagttcgcgt cgggtcatca ggaatcgctc attcatcgtc tgctgcctcg gtatcaggaa
     1021 gggaaagcgg gcggaaaccg cccgtcgcgg cacgtcgtca gggagggttc atgcccgctt
     1081 ccggcgcggg cgatgccgcc gggccggggg agccggaaat ggctgcggac ggcagggcgg
     1141 cgccctcagg ccggaaacgc ccggtagccg gccgatggct gcggggcggc ccagacctgc
     1201 gccccggccg gaagcagtgc ctcgccgcgc tgacgcggca cgctgacctt cagcgccgcg
     1261 ccgccctcga gcgcgaggtc gtagacgacg agcgcaccga gcggcagcgc cgtctgcacc
     1321 gcgccgggca gcgcatcggc gcccggtgcc tcggcgtgca ggtgcaggtg ctcggggcgg
     1381 atgctgacgc gcaccgcgct gccggccgtc agcggcgcat cgctggcgac ccggatgcgc
     1441 cggccgccgg ccagcgccac gaccgggctg ccgtcggcat cggccgtgct gaccgtgccg
     1501 tcgagcacgt tggtcgagcc gacgaactgg ttcacgaaca gcgtcgcggg ccggtcgtag
     1561 atatcggtcg gcgaggcgat ctgctcgacg cggccgcggc tcatgaccgc gacgcgatcg
     1621 gccatcgaca gcgcctcctc ctggtcgtgg gtcacgagca cggtggtgat gccgtactcg
     1681 cgctgcaggc gcttgacctc gatctgcatg tcaaggcgca ggttcttgtc gagcgccccg
     1741 aagggctcgt cgagcagcag gatcttcgga tcgacggcaa ggcagcgcgc gagcgccacg
     1801 cgctgctgct ggccgcccga aagctggcgc gggaagcgcc cggcgaactc gctcatgtgc
     1861 acgagcgcga gcatctcgtc gacgcgcgca tcgcgcttcg ccttcgccac gccctgcact
     1921 tcgaggccgt aggcgatgtt ctggcggatg ctcaggtgcg ggaacagcgc gtagttctgg
     1981 aacacgatgc cgacgccgcg ccggctcggc gacaggtgat cgaccggctc gccgtcgaac
     2041 agcacccggc cctcggtctg gcgcatgaag ccggagatga tccgcagcag cgtcgacttg
     2101 ccgcagcccg aaggcccgag cagcgccacc agttcgccgg caccgatgcg caggttgatg
     2161 tcacgcaccg ccacgaagtc gtggaagcgc tgggtgatgg cttcgagctg cagctcgtgt
     2221 ccgcgttgtc cgctcatggc gatcagctcc cccggtaggt ggtgtaactg gcaggcgtgc
     2281 acagcagcgg cacgtggtag tgcagggccg catcggagac gacgaaacgc accggcaccg
     2341 tgtcgaggaa accgccgccg tcgtgaccgc ggctgcggaa gtagtcgccg atgcggaatt
     2401 cgatctggta ggtgccggcc cgcatcgcgt cgtcacagag cagcagcggg cagcggccct
     2461 cggcgtcggt ctcgaagccg ccgagccggc gccagcctcc ggcgtgctgc accgacagcg
     2521 tcagcgccac gccggcggcc ggcaggccgg cgaccgcgtc gagcacatgc gtgctcagcc
     2581 agcccggcgc gatcccgaag cgctgcgcca gccgcccgcg cacgacctcg gccacctgcg
     2641 tgagcgcgac cccggcctcg gtggccgcgg cgttcgcgag ccggcgttcg ccctcggcga
     2701 agatcgaggc gagatccgcg tgcaggcgca cggccgtgat gaacggaaag ccgaaacgct
     2761 cgcggtaggc ggtgttcagc gccctcaggc gggcgtgctc ggccgcactg aggcggtcga
     2821 ggccgaggcg cgactgctcg cggttcgaat ccgcggtcag cgtgccctcg cccgcctcgc
     2881 gcccggccag ttccggatga cgctgcagca gcgcggtgcg cgtgtcggca tcgctggcgc
     2941 gcagcacgcc ggcgagcgcc gcatgcagcg cccacaccga cgcgaacggc cgcagcgcgg
     3001 cgacgcggcg cacgacccac ggggagtgtt cgacgacctc gccgagggcc gcgacgaagg
     3061 cgtcctccgg gcaggcattc agggcttcga gctgggcggc cgcgtcgcgg tccggaatcg
     3121 gaatgtccac cgtgcgcctc gtctgttgca gtgcgacagg cgcaactctg gcgctcggcg
     3181 ggcattgccg tccatctgat aataagcaag gctgcgttcg ataaattcga acgggaggac
     3241 gcgatgcccc agtccctgct gccgaagatg ctgcgctatg ccgaggtcgt cgcccgtgcc
     3301 ggttcggtgc aggccgccgc gcgcgtgctg aacctgtcgg cctcggcgat cgaccgccag
     3361 atcctgctgc tggaggatct gctcggcgcc ccgctgttcg agcgccagcc cgacggcatg
     3421 cgcctcagtg ccgccggcga gctggtggtg acgctggcga agcgctggca gcacgatgcc
     3481 agccggctgc tgaccgacat ccgccagctg cagggcatca atcagggcag cctgcggctc
     3541 gcggcgatgg acagccacgc caacggcctg atgccggtgt tcctcgaacg cctgcaccgc
     3601 gagcacccgc gcatccggct ggagatcgac atcgtcagcc ccgatcaggc cgaggccgcg
     3661 ctgatcgccg gcacgaccga cctcgcgatc gcgttcaacc tgaaggccgg tcgcgacctg
     3721 cacctggtca tgcaggccga actgccgctc ggctgcgtcg tcgcgcccgg ccacccgctc
     3781 gccggccagc ccgacaccag cctgaaggcg gtctccggat atccgatcgt ctcgcagaac
     3841 cgctcgctga cgatccgccg ctacctggag agccgccacg gctggctgtt cgccgaacgc
     3901 gaggccccgg tgcagaccaa ctcgctgcag ctggtcaagc acctcgccgc cgccggcagc
     3961 cacgtcgccc tgacctcgga actcgacgcc gctcccgaaa tcctcgccgg gcgtctgtgc
     4021 ttcatcccgc tgaccgaccg caacgtgcag ccgcagacgg tcgcgatcgc aatcagcgcc
     4081 cgccggccct tgccgcgcat cggacggctg gtggcggatt tgctggcgga ggaattgcgg
     4141 ggctgtctgg aggccgtgcg gcaacggcgt gcggccggca gcggcggctg agcggggccg
     4201 gcgctgattt gcggcccatg aaaaagccgc cggttccctg cggggaaccg gcggctcttg
     4261 ccagtcaatg gcggagaagg agggattcga accctcgata cgcggttagc gtatacacga
     4321 tttccagtcg tgcgccttcg accactcggc cacttctccg gaattccgta ctcgccgcaa
     4381 ggtggcggcg agcgcgcgag agtacggcat catccgcacc gatcgcaagc gattcagcga
     4441 cgatcggtac agatgtccgc cggcctcagg cgtcgacctg acgcatggtc aggcgcacgc
     4501 ggccctgctt gtcgatttcc atgaccttga cgcgcacgct gtcgccgacg gcgagcttgt
     4561 cggtgacgct ctggatgcgc tcctcgcaga tctgcgagat gtgcaccatg ccgtcacgcc
     4621 cgggcagcag cgacacgaag gcgccgaagt ccatgatgcg cacgaccttg ccttcgtaga
     4681 tgcgaccgac ctcgatgtcg gcggtgatca tctcgatgcg ccggcgcgcc tcgtcgctgg
     4741 cgctcttgtc gaccgcggcg atcttgaccg tgccgtcgtc gtcgatgtcg atcgtcgtgc
     4801 cggtttcctc ggtgagctgg cggatgaccg cgccgccctt gccgatcacg tcacggatct
     4861 tgtcgggatt gatcttgatc gtgacgatgc gcggtgcgaa cgacgagatg tcgtcgcgcg
     4921 gcctgtcgag cacggcgttc atgcagccga ggatgtgcag gcggccttcg cgggcctggg
     4981 ccagcgcgac ctgcatgatc tccttggtga tgccgtcgat cttgatgtcc atctgcagcg
     5041 cggtgacgcc ctgcgtggtg ccggcaacct tgaagtccat gtcgccgagg tggtcttcgt
     5101 caccgaggat gtcggagagc accgcgaagc gctcgccttc cttgatcaga cccatcgcga
     5161 tgccggccac cggtgccttc agcggcacgc cggcgtccat cagcgccagg ctggagccgc
     5221 agaccgaggc catcgagctg gagccgttcg actcggtgat ctcggagacc acgcgcacga
     5281 cgtacgggaa cttctccatg tccggcatca cggccgcgac gccgcgcttg gccagacgcc
     5341 cgtggccgat ctcgcggcgc ttcgggctgc cgacgcggcc ggtctcgccg accgagaacg
     5401 gcgggaagtt gtaatgcagc atgaagagct cgcggtactc gccttcgagc gcgtcgatga
     5461 tctgcgagtc gcgggtggtg ccgagcgtgg cgacgaccag cgcctgcgtc tcgccgcgcg
     5521 tgaacagcgc cgagccgtgc gtgcgcggca gcacgccggc gcgcacggtg atcgggcgga
     5581 ccgtcttggt gtcgcggccg tcgatgcgcg gattgccgtc gaggatgcgg tcgcgcacga
     5641 cgcggtattc gaggttcgaa taagccgtct tgacggcttc cggcgtgtag gcggcacctt
     5701 caccggcgag ctgctcgacc accgagccgc ggatttcgcg cacgcggttc tggcggtcct
     5761 gcttgacggc gatctggtag gcctcgacca gcgatccgaa ggcggcgctg cgcacggcgg
     5821 catcgagcgc ggtatcggcg gccggggccg accagtccca ggccggcttg cc
//
LOCUS       DAOQJH010000057         5839 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_7813_length_5839_cov_4.107538, whole genome shotgun sequence.
ACCESSION   DAOQJH010000057 DAOQJH010000000
VERSION     DAOQJH010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 5839)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5839)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5839
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7813_length_5839_cov_4.107538"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..944)
                     /locus_tag="PLQ18_02310"
     CDS             complement(<1..944)
                     /locus_tag="PLQ18_02310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE00512.1"
                     /translation="MLTHERERVLAGHRWRFQRAGGYDHVCLDTGADLVALPTLDQKL
                     WAALACPTRGVEFDARTLDLIDTDADGRIRAPEVLAAVDWALARLRDPDTLLAGGEAL
                     ALGAIDTRHEDGPDSGRLIHACACEVLRQLGRAEAGTIELDDVADTHRLYATIPFNGD
                     GVITAAAAGDDEALRAALLDVLQVCGGVPDRSGVDGIDRERLQGCFAALDALAAWRDD
                     GGSGPVRPLGADTAAAAEALAAVQAKIDDYFLRCRLAAWDAALLDALQQDAGERTLAE
                     ALKPVRDAEALAALPLDRAAPGRPLDFEAALNPAWLGRL"
     gene            complement(988..1470)
                     /locus_tag="PLQ18_02315"
     CDS             complement(988..1470)
                     /locus_tag="PLQ18_02315"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016488639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HNE00513.1"
                     /translation="MERTGIGQTVAPFTAAATGGETVDSAALRGRWLLLYFYPKDATP
                     GCTSEGRDFRELHPEFLAAGAVLYGVSRDSLASHEKFRAKEDFPFALISDPDETLCRL
                     FDVIRPKKLYGKDVIGIERSSFLIDPDGVLRREWRKLRVEGHARAVLAALREELAATG
                     "
     gene            complement(1476..2006)
                     /locus_tag="PLQ18_02320"
     CDS             complement(1476..2006)
                     /locus_tag="PLQ18_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015281789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein R"
                     /protein_id="HNE00514.1"
                     /translation="MKNYLVINVLGEDRPGLLLDLSRIILDCGCSIQDSYITVFGLEC
                     VAMLLVHGNWNTLAKLETQLKRAEEEHGLTITIKRTGPRKISQDALPYSVEVIALEAP
                     GIVNHLAAFFASRSINIEELSTRSYSAPHTGSPMYSVNMAIGIPASLHVALLREEFLD
                     YCDELNLDAVMEPVKG"
     gene            2169..3044
                     /gene="dapA"
                     /locus_tag="PLQ18_02325"
     CDS             2169..3044
                     /gene="dapA"
                     /locus_tag="PLQ18_02325"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017364904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HNE00515.1"
                     /translation="MFHGSMVALVTPMSADGALDFDALARLVEFHIENGTDALVAVGT
                     TGESATLDVPEHIEVVRRVVKQVAGRVPVIAGTGANSTREAIELTRLAMEAGADACLL
                     VTPYYNKPTQEGLYRHYRAIAEAVPIPQILYNVPGRTACDMKPETVARLADIPNIVGL
                     KEASGSLERNRELIARCAGRIDLYSGDDDLGCASILAGFRGVISVTANVAPRAMHEMC
                     KAALAGDAASAEAINADLTGLHKQLFIESNPIPAKWALQRLGLIPSGIRLPLTPLSES
                     CHEAVLDAMRQAHVL"
     gene            3078..4277
                     /gene="bamC"
                     /locus_tag="PLQ18_02330"
     CDS             3078..4277
                     /gene="bamC"
                     /locus_tag="PLQ18_02330"
                     /inference="COORDINATES: protein motif:HMM:NF018509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HNE00516.1"
                     /translation="MPKLHTPLRVAAAGTTLALLAACSSPSGSLIPDRRPDYRQAREA
                     PTLELPPDLTSSTIDDTLKVPELNPGRQANLSDYNRERGVAGGAVASTAAGTAPRLVK
                     TESVLLQPEGMRIERQGEQRWLVVDAPADKVWPKVREFWTSNGFLLKRDDPRIGLLET
                     DWAENRADIPQDAIRALISKALDFAYSAPTRDKFRVRLERTGDGSGTAVYLTHYGAKE
                     VSRGRDNESTVWEPRPEDPELEAELLSRLMVFLGASEERARAQAGVGQKAAPVQKALT
                     RPGELGGVPGLFVTQDYSRAWRSVGAALDGGDYGIEEQDRARGLYIVEYVDRAGENRK
                     ADDNKGWFSGWFGSKSAPQPRGTRYQVRLADQGGETFVIVQNASGTPDNSAGARTLLE
                     TIARGVR"
     gene            4360..>5839
                     /gene="mutL"
                     /locus_tag="PLQ18_02335"
     CDS             4360..>5839
                     /gene="mutL"
                     /locus_tag="PLQ18_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019465491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HNE00517.1"
                     /translation="MPIRRLPPELVNQIAAGEVVERPASVVKELLENSLDAGATRIDI
                     DIEQGGVRLIRVRDDGCGITREELAAALASHSTSKISTAADLDAIASLGFRGEALPSI
                     AAVSRLTLASRTDGAASGWQLAGDGREQPGEPLPAALAARGTAIEVRDLFHNVPARRK
                     FLKAERTEFGHIDELVRRLAASRPAVAFTLSHNGRALLRLPAAADRAGEERRIAELAG
                     SDFLGAALHVDHTAAGLRLHGWVGLPTAARAQADRQFFYVNGRMVRDRLVAHALRQAY
                     ADVLFHGRHPAFVLQLELDPQLVDVNAHPQKFEVRFREARLVHDFLFRTLHEVLADTR
                     AGAGAGAGAPAAVGVAAGALPDPEMPVPPDAGRWPPPAAPAPQQNRLALGAHESFAVY
                     ARLLDGLAPAPAQAGLAAVEPDATAAPPAAVPPLGYALAQLHGIWILAENAAGLVLVD
                     MHAAHERITYERLKRAHAAEGLRTQPLLVPLGFAVGAREAAAI"
BASE COUNT          834 a         2087 c         2096 g          822 t
ORIGIN      
        1 agccgtccga gccaggccgg gttcagcgcc gcctcgaaat cgagcgggcg tccgggggcg
       61 gcgcgatcga gcggcagcgc cgcgagcgcc tcggcatcac gcaccggctt cagcgcctcg
      121 gcgagcgtgc gctcgccggc atcctgctgc agcgcgtcga gcagcgcggc atcccaggcg
      181 gcgagccggc agcgcaggaa gtagtcatcg atcttggcct gcacggcggc gagcgcctcg
      241 gccgcggcgg cggtgtcggc cccgagcggg cgtaccggtc cgctgccgcc gtcgtcgcgc
      301 caggcggcga gcgcatcgag ggcggcgaag cagccctgca ggcgctcgcg gtcgatgccg
      361 tcgacgccgc tgcgatccgg gacgccgccg cagacctgca gcacgtcgag cagcgcggcg
      421 cgcagggctt cgtcatcgcc ggcggcggcc gcggtgatca cgccgtcgcc gttgaacggg
      481 atcgtcgcgt acagccggtg cgtgtcggcg acatcgtcga gctcgatcgt gccggcttcg
      541 gcgcgcccga gctggcgcag cacctcgcac gcgcaggcgt ggatcagccg gccgctgtcg
      601 gggccgtcct cgtggcgcgt gtcgatcgcg cccagcgcca gtgcctcgcc gccggcgagc
      661 agggtgtcgg ggtcgcgcag ccgtgcgagc gcccagtcga cggcggcgag cacttccggc
      721 gcgcggatgc ggccgtcggc gtcggtgtcg atcaggtcga gcgtgcgcgc gtcgaactcg
      781 acgccgcgcg tcgggcaggc gagcgcggcc cagagcttct ggtccagcgt cggcagcgcg
      841 acgaggtcgg cgccggtatc gagacagaca tggtcgtaac cgccggctcg ctggaaacgc
      901 cagcgatgac cggccagcac gcgctcgcgc tcgtgcgtca gcatgggcag gactccgcga
      961 ccggatgaag gatcgcgcgg gcagacctca gccggtggcg gcgagttctt cgcgcaaggc
     1021 agcgagcacg gcgcgggcgt ggccctcgac cctgagcttg cgccattcgc ggcgcagcac
     1081 gccgtcggga tcgatcagga agctgctgcg ctcgatgccg atcacgtcct tgccgtagag
     1141 cttcttcggc cggatcacgt cgaacagccg gcagagggtt tcgtcggggt ccgagatcag
     1201 cgcgaacggg aagtcttcct tcgcgcggaa tttctcgtga ctcgccagcg aatcgcgcga
     1261 gacgccatag aggacggcgc cggcggcaag gaattccggg tgcagctcgc ggaagtcgcg
     1321 gccctcgctg gtgcagccgg gggtcgcgtc cttcggatag aagtacagca gcagccagcg
     1381 tccgcgcagc gccgccgagt cgacggtttc gccgccggtc gcggcggcgg tgaacggtgc
     1441 cacggtctgg ccgatgccgg tgcgttccat cgccttcagc ccttgaccgg ttccatgacc
     1501 gcgtcgaggt tcagctcgtc gcagtagtcg aggaattcct cgcgcagcag cgccacgtgc
     1561 aggctggccg ggatgccgat cgccatgttc accgagtaca tcgggctgcc ggtgtgcgga
     1621 gccgagtagc tgcgcgtcga gagctcctcg atgttgatgc tgcgcgaggc gaagaaggcc
     1681 gcgaggtgat tgacgatgcc cggcgcctcc agcgcgatga cctcgacgct gtacggcagg
     1741 gcgtcctgcg agatcttgcg cgggccggtg cgcttgatcg tgatcgtcag gccatgttcc
     1801 tcctcggcgc gcttgagctg ggtttcgagc ttcgcgagcg tgttccagtt gccgtgcacc
     1861 agcagcatcg ccacgcattc gaggccgaac accgtgatgt agctgtcctg gatgctgcag
     1921 ccgcagtcga ggatgatgcg cgacaggtcg agcagcagac ccgggcgatc ctcaccgagc
     1981 acgttgatga cgagatagtt cttcacgggg gcgcgcatgc gtcgggggca gtggactggg
     2041 gtgcgatgag gcggacgggg cagtgtagcg gctcgccggg ggtgtgcaag ccgcgttccg
     2101 gccggcgctt gtgaccggca ccggcggcac gtaccatgcc gcacattttc cttgcggggt
     2161 tcccggacat gttccacggc agcatggtcg cgctggtcac gccgatgtcg gcggacggcg
     2221 ccctcgactt cgatgcgctc gcacgcctcg tcgagttcca catcgagaac ggcaccgacg
     2281 cgctggtcgc ggtcggcacc accggtgaat cggcgacact cgacgtgccc gagcacatcg
     2341 aggtcgtgcg ccgcgtcgtg aagcaggtgg ccgggcgcgt gccggtgatc gccggcaccg
     2401 gcgccaattc gacgcgcgag gcgatcgagc tgacccggct ggccatggag gccggtgccg
     2461 acgcctgcct gctggtgacc ccgtactaca acaagccgac ccaggaaggc ctgtaccggc
     2521 actaccgcgc gatcgccgag gccgtgccga tcccgcagat cctctacaac gtgcccgggc
     2581 gcaccgcctg cgacatgaag cccgagaccg tcgcgcgtct ggccgacatc ccgaacatcg
     2641 tcggcctcaa ggaggcgagc ggctcgctgg agcgcaaccg cgagctgatc gcgcgctgcg
     2701 ccgggcgcat cgacctctac agcggtgacg atgacctcgg ttgcgcgagc atcctcgccg
     2761 gtttccgcgg cgtgatctcg gtgaccgcca acgtcgcacc gcgcgcgatg cacgagatgt
     2821 gcaaggctgc gctcgccggc gacgccgcca gcgccgaggc gatcaacgcc gacctgaccg
     2881 gcctgcacaa gcagttgttc atcgaatcca atccgattcc ggccaagtgg gcgctgcagc
     2941 gcctggggct gatcccgtcc ggcatccgcc tgccgctgac gccgctgtcg gagtcctgtc
     3001 acgaagccgt gctcgacgcg atgcgtcagg cccacgtgct ctgaacatcc cgtccgccgc
     3061 cgacgaaccc tcgccccatg cccaagctcc ataccccgct gcgcgtcgct gccgccggca
     3121 cgacgctggc cctgctcgcc gcctgctcct ccccgagcgg cagcctgatt cccgaccgtc
     3181 gccccgatta ccgccaggcc cgcgaggcgc cgacgctgga actgccgccg gacctgacca
     3241 gctcgacgat cgatgacacg ctgaaggtgc cggagctgaa cccgggccgg caggcgaacc
     3301 tctccgacta caaccgcgaa cgcggcgtgg ccggcggagc cgttgcgagc actgccgccg
     3361 gcacggcgcc gcggctggtc aagaccgaat cggtgctgct gcagcccgag ggcatgcgca
     3421 tcgagcgtca gggcgagcag cgctggctgg tggtcgacgc gccggccgac aaggtctggc
     3481 cgaaggtgcg cgagttctgg accagcaacg gcttcctgct caagcgtgac gatccgcgca
     3541 tcggcctgct ggagaccgac tgggccgaga accgcgccga catcccgcag gatgccatcc
     3601 gcgcgctgat ctcgaaggcg ctcgacttcg cgtactcggc tccgacccgc gacaagttcc
     3661 gcgtgcgcct cgaacgcacc ggcgacggca gcggcacggc cgtctacctg acccactacg
     3721 gtgccaagga agtcagccgc ggtcgcgaca acgaatcgac ggtctgggaa ccgcgcccgg
     3781 aagacccgga actcgaagcc gaactgctgt cgcggctgat ggtgttcctc ggggcttccg
     3841 aggagcgcgc ccgcgcccag gccggcgtcg gccagaaggc cgcgccggtg cagaaggcac
     3901 tgacccgtcc cggcgagctc ggcggcgtgc cgggcctgtt cgtcacgcag gactactcgc
     3961 gcgcctggcg cagcgtcggt gcggcgctcg acggcggtga ctacggcatc gaggagcagg
     4021 atcgcgcccg cggcctgtac atcgtcgagt acgtcgatcg cgccggcgag aaccgcaagg
     4081 ccgacgacaa caagggctgg ttctcgggct ggttcggctc gaagtcggcg ccgcagccgc
     4141 gcggcacgcg ctatcaggtg cgcctcgccg atcagggtgg cgagaccttc gtcatcgtgc
     4201 agaacgccag cggcacgccg gacaactccg ccggggcccg cacgctgctc gaaaccatcg
     4261 ccaggggtgt gcgctgagcc gccgcctgcc cgcatcgtcc cctgcacggg ccggccatcc
     4321 gccggcccgt ggcatttccg gagctgcccg gagaccgcca tgccgatccg tcgcctgccg
     4381 cccgaactcg tcaaccagat cgccgccggc gaggtcgtcg agcgcccggc ctcggtggtc
     4441 aaggagctgc tggagaacag cctcgatgcc ggcgccacgc gcatcgacat cgacatcgaa
     4501 cagggcggcg tgcggctgat ccgcgtgcgc gacgacggct gcggcatcac ccgcgaggag
     4561 ctcgccgcgg cgctggcgag ccactcgacc agcaagatca gcacggcggc cgatctcgac
     4621 gcgatcgcga gcctcggctt tcgcggcgag gcgctgccga gcatcgctgc ggtatcgagg
     4681 ctgacgctcg cctcgcgcac cgacggtgcc gccagcggct ggcagctcgc cggcgacggc
     4741 cgcgagcagc ccggcgaacc gctgccggcg gcgctggcgg cgcgcggcac ggcgatcgag
     4801 gtgcgcgacc tgttccacaa cgtgccggcg cggcgcaagt tcctgaaggc cgaacgcacc
     4861 gagttcggtc atatcgacga gctggtgcgc cggctcgccg cctcgcggcc cgcggtcgcg
     4921 ttcacgctga gccacaacgg ccgggcgctg ctgcgcctgc cggcggcggc ggatcgcgcc
     4981 ggcgaggagc ggcgcatcgc cgagctggcc ggcagcgatt tcctcggcgc ggcgctgcac
     5041 gtcgatcaca cggccgccgg cctgcgcctg cacggctggg tcgggctgcc gacggcggcg
     5101 cgcgcgcagg ccgaccggca gttcttctat gtcaacggcc gcatggtccg cgaccggctg
     5161 gtcgcgcacg cgctgcgcca ggcctacgcc gacgtgctgt tccacggccg gcatccggcc
     5221 ttcgtgctgc agctcgaact cgacccgcag ctggtcgatg tcaacgccca tccgcagaag
     5281 ttcgaggtgc gcttccgcga ggcgcggctg gtgcatgact tcctgttccg cacgctgcac
     5341 gaggtgctgg ccgacacgcg cgccggcgca ggcgccggcg caggcgctcc ggctgctgtg
     5401 ggtgttgcgg ccggcgctct gccggatcct gaaatgccgg tcccgcctga tgcgggtcgc
     5461 tggccgccgc cggccgcgcc cgcgccgcag cagaaccgcc tcgccctcgg tgcgcacgag
     5521 tcgttcgcgg tctatgcgcg cctgctcgac gggcttgcac cggcaccggc gcaagccggg
     5581 cttgccgcgg tggagccgga cgcgacagcc gcgccgccgg ctgcggtgcc gccgctcggc
     5641 tatgcgctcg cgcagctgca cggcatctgg atcctcgccg agaatgccgc cgggctggtg
     5701 ctggtcgaca tgcatgccgc gcacgagcgc atcacctacg agcgcctgaa gcgcgcgcac
     5761 gcggccgaag gcctgcgcac gcagccgctg ctggtgccgc tcggcttcgc ggtcggcgcg
     5821 cgcgaagcgg cggcgatcg
//
LOCUS       DAOQJH010000058         5808 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_7897_length_5808_cov_4.764992, whole genome shotgun sequence.
ACCESSION   DAOQJH010000058 DAOQJH010000000
VERSION     DAOQJH010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 5808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5808
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7897_length_5808_cov_4.764992"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1404)
                     /locus_tag="PLQ18_02340"
     CDS             complement(<1..1404)
                     /locus_tag="PLQ18_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019485271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNE00518.1"
                     /translation="MVPPPAGAPASAWSPLRHRTFRWLWLAAIASNIGTWMHEVGAGW
                     LMTTLSPSPLLVSLVQAAGAAPMFVLALPAGALSDIVDRRRFLIGVQIWMALVAAALA
                     AATLTGVMTPGLLLGLTAALGIGSALMAPAWAALTPELVPKPELQSAIALSSVGINVA
                     RAIGPALAGLIVSSVGPWATFALNAASFAGVIIVLAGWRRPQAVATLPSERFFGALRA
                     GWRYARSAPRLQAVIVRALAFFVFASAGLGLLPLLVRRLPDGSAGVYGALLACVGVGA
                     VGGAFLLPKLRERVSADQLVAGASALYAGVLLALALLPSAPWLAPVMLASGAAWIAVL
                     SSFQVAAQTAAPPWVRARALAVYMLAFNGAMAAGAALWGTIAAHQAPAVALVAAAAGL
                     LLLILPVRRFRLACAAADDLSPSLHWPMPLAEGAAIGHDRGPVLVTVEYEIPPAHAAA
                     FVQAMQAMARVRRRNGAL"
     gene            complement(1483..3369)
                     /locus_tag="PLQ18_02345"
     CDS             complement(1483..3369)
                     /locus_tag="PLQ18_02345"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017677264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HNE00519.1"
                     /translation="MSADASAAPSADSGSGAELILLNGRFHTVDRERPDASAVAIRAG
                     RFVAVGDAAEVMRWQGPASVVVSLDGHCAIPGLIDSHLHLIRGGLNYNLELRWEGLPS
                     LADALALLRRQAQRTPPGQWLRVVGGWTEFQFAEKRLPTLAELNAAAPETPVFVLHLY
                     DRALLNRAALRAVGYTRDTPEPPGGAIERDAAGEPTGLLLAKPNAMILYATLAKGPKL
                     PLEQQANSTRHFMRELNRLGVTSAIDAGGGFQNYPDDYAVIDRLAADGELTVRIAYNL
                     FTQRPGQELDDFTHWTETVPLRQGDDWYRHNGAGEMLAFSAADFEDFVMPRPELPVRM
                     ETELEAVVRRLVAGRWPFRLHATYDESITRMLDVFERVDRDLPFAGLPWFFDHAETVS
                     PRNLERIRALGGGIAIQHRMAFQGEYFVERYGAEAARATPPVARMLELGIPVGAGTDA
                     TRVASYNPWTALYWLVSGRTVGGLALAPHGLARDTALELWTAGSAWFSSDQGNKGRIR
                     VGELADLAVLSADYFSVPEEDIRAIESLLTVVGGRIVHAAGGFAPLAPPPLPVLPEWS
                     PVAIAPGHHRPAPPAAALPHQCLGACAVHGHAHDRARRAAVPVAAGGLQGFWGAFGCS
                     CFAF"
     gene            complement(3430..4050)
                     /locus_tag="PLQ18_02350"
     CDS             complement(3430..4050)
                     /locus_tag="PLQ18_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017904486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HNE00520.1"
                     /translation="MNAAAPAAAAPEPPEPSEPPAAVFGGAHAGHVSFVIRHRVEPRR
                     RADYERWLTRIMPVAATYPGHLGVSVIRPDAPGTPYTIVLRFERLEQLRRWMESADRR
                     ELIEQIRPLLAAGEDLDIRPGAAFWFTPAGAQPPRLWKQVLMTFAVIWPLTLIVPQLW
                     QPAFTRWPWLGGWLAGNFVVALSIVLIVVCAVMPWLTRRLAGWLTR"
     gene            complement(4047..4718)
                     /locus_tag="PLQ18_02355"
     CDS             complement(4047..4718)
                     /locus_tag="PLQ18_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009923512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HNE00521.1"
                     /translation="MTDRTALEQLTPDNSVLLFIDHQPQMAFGVANIDRQTLKNNVVA
                     LAKSAKVFGVPTFITTVETESFSGYTWPELLDVYPDAALLERTSMNSWDDAKVRDAIN
                     GSGRRKLILSGLWTEVCINSAANSAMLDGYEVYVVADACGGTSKDAHDYSMLRMIQAG
                     IRPLTWQQTLLEWQRDWARREHYDEVMAIVREHSGAYGMGVDYAYTRVHGAQQRGQWR
                     GSVRK"
     gene            4957..>5808
                     /locus_tag="PLQ18_02360"
     CDS             4957..>5808
                     /locus_tag="PLQ18_02360"
                     /inference="COORDINATES: protein motif:HMM:NF018239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XylR N-terminal domain-containing protein"
                     /protein_id="HNE00522.1"
                     /translation="MTDPSPPNGPAFAALRADIEHLLQRFTLGEHGGRMLLQGRRMVV
                     QSARSLGQLREELIRMLGADLARGVLKRYGYQSGFQDGHALRLRHPELPVELQMHLGT
                     VLHEREGHARIETEPEGTHVDLARGVLDIRSRWHDSVEAEQHHLLLGRSSEPVCWTLS
                     GYASGHLSYLLQREVVIVETACRAQGHPYCRFRVGFREDMEKHFPGCADDYRRLDLGR
                     LFRELEQTVEAQSRTIRELREQLGAAPAAGAAASAAFSTLTGRSPALARAIDIARAVA
                     GVSSTVLV"
BASE COUNT          841 a         2058 c         2156 g          753 t
ORIGIN      
        1 cagcgcgccg ttgcgccggc gcacgcgcgc catcgcctgc atcgcctgca cgaaagcggc
       61 cgcgtgcgcg ggcggaatct cgtattcgac cgtcaccagc accggcccgc ggtcgtgccc
      121 gatggccgcg ccttcggcca gcggcatcgg ccagtgcagc gacggcgaca ggtcatcggc
      181 cgccgcgcag gcgagccgga agcggcgcac cggcaggatc agcagcagca gcccggccgc
      241 ggcggcgacg agggccacgg ccggcgcctg gtgcgcggcg atcgtgcccc agagcgccgc
      301 gccggcggcc atcgcgccgt tgaacgcgag catgtagacg gcgagcgcgc gtgcccgcac
      361 ccacggcggc gcggcggtct gcgcggcgac ctggaacgag gacagcacgg cgatccaggc
      421 cgcaccgctg gccagcatca ccggcgccag ccacggtgcc gacggcagca gcgccagcgc
      481 gagcagcacg ccggcgtaca gcgcgctcgc cccggccacg agctggtcgg ccgacacgcg
      541 ctcgcgcagc ttcggcagca ggaaggcgcc gccgaccgcg ccgacgccga cgcaggcgag
      601 cagcgcgccg tagacgccgg cgctgccgtc cggcagccgc cgcaccagca gcggcagcag
      661 gccgagcccg gcgctcgcga acacgaagaa ggccagcgcg cgcacgatca ccgcctgcag
      721 ccgcggggcg ctgcgcgcgt agcgccagcc ggcacgcagc gccccgaaga agcgctcgga
      781 cggcagcgtc gccaccgctt gcggccggcg ccagccggcg agcacgatga tgacgccggc
      841 aaacgacgcg gcgttcagcg cgaaggtcgc ccacggcccg acgctgctga cgatcagccc
      901 ggccagcgcc ggcccgatcg cgcgcgcgac gttgatgccg acgctggaga gcgcgatcgc
      961 cgattgcagc tcgggcttcg gcaccagctc cggcgtcagc gccgcccagg ccggcgccat
     1021 cagcgccgag ccgatgccga gtgccgcggt cagcccgagc agcagccccg gtgtcatcac
     1081 gccggtgagc gtcgccgccg cgagtgcggc ggcgaccagc gccatccaga tctgcacgcc
     1141 gatcaggaag cgccggcgat cgacgatgtc ggacagcgcg ccggccggca gcgccagcac
     1201 gaacatcggc gccgccccgg cggcctgcac cagcgacacc agcagcgggc tcggcgacag
     1261 cgtcgtcatc agccagccgg cgccgacctc gtgcatccag gtgccgatgt tcgacgcgat
     1321 cgccgccagc cacagccagc ggaaggtgcg atggcgcagc ggactccagg cgctggccgg
     1381 cgccccggcc ggcggcggga ccatggcatc cgcggtcgtg gcgggagctt cggagcgcat
     1441 cggatcgttt ccttcaggcc ggcggcggcg gtgcgcgccg cttcagaacg cgaagcacga
     1501 gcagccgaac gcgccccaga agccctgcag tccgccggcg gcgaccggga ctgcggcgcg
     1561 gcgggcgcgg tcgtgggcgt ggccgtgcac ggcgcaggcc cccaggcact ggtgcggcag
     1621 cgcggcggcg ggcggagcgg ggcggtgatg gccgggcgcg atcgcgaccg gtgaccattc
     1681 cggcagcacc ggcagcggcg gcggcgccag cggggcgaag ccgcccgcgg cgtgcacgat
     1741 gcggccgccg acgacggtca gcagcgattc gatcgcgcgg atgtcctctt ccggcaccga
     1801 gaagtaatcg gccgacagca cggccaggtc ggccagctcg ccgacgcgga tgcggccctt
     1861 gttgccctga tccgacgaga accaggcgct gccggccgtc cagagttcga gcgcggtgtc
     1921 gcgcgcgagg ccgtgcgggg ccagcgccag gccgccgacc gtgcggccgc tgaccagcca
     1981 gtacagcgcc gtccacgggt tgtagctcgc cacgcgcgtg gcatcggtgc cggcgccgac
     2041 cgggatgccg agttcgagca tgcgcgccac cggcggcgtc gcgcgcgcgg cctcggcgcc
     2101 gtagcgctcg acgaagtact cgccctggaa ggccatgcgg tgctggatgg cgatgccgcc
     2161 gccgagtgcg cggatgcgtt cgaggttgcg cgggctgacc gtctcggcgt ggtcgaagaa
     2221 ccacggcagg ccggcgaacg gcaggtcgcg atcgacgcgc tcgaacacgt cgagcatgcg
     2281 cgtgatcgat tcgtcgtagg tcgcgtgcag ccggaacggc cagcgtccgg cgacgagccg
     2341 gcgcacgacg gcttcgagct cggtctccat gcgcaccggc agctccgggc gcggcatcac
     2401 gaagtcctcg aagtcggcgg ccgagaacgc cagcatctcg ccggcgccgt tgtgccggta
     2461 ccagtcatcg ccctggcgca gcggcacggt ttcggtccag tgcgtgaagt cgtcgagttc
     2521 ctgcccgggg cgctgcgtga acaggttgta ggcgatgcgc acggtcagct cgccgtcggc
     2581 ggcgaggcgg tcgatcaccg cgtagtcgtc cgggtagttc tggaagccgc cgccggcgtc
     2641 gatggcgctg gtgacgccga ggcggttcag ctcgcgcatg aagtggcgcg tcgagttggc
     2701 ctgctgctcc agcggcagct tcgggccttt cgccagcgtc gcgtacagga tcatcgcgtt
     2761 cggcttcgcg agcagcaggc cggtcggctc gccggcggcg tcgcgctcga tcgcgccgcc
     2821 gggcggctcg ggcgtgtccc tggtgtagcc gacggcgcgc agcgcggcgc ggttgagcag
     2881 tgcgcggtcg tacaggtgca gcacgaacac cggcgtttcc ggtgccgcgg cgttcagctc
     2941 ggcgagcgtc ggcaggcgct tctcggcgaa ctggaattcg gtccagccgc cgaccacgcg
     3001 cagccactgc ccgggcggcg tgcgctgcgc ctggcggcgc agcagcgcca gcgcatcggc
     3061 gagcgagggc aggccctccc agcgcagttc gaggttgtag ttcaggccgc cgcggatcag
     3121 gtgcaggtgc gagtcgatca ggccgggaat cgcgcagtgg ccatcgaggc tgacgacgac
     3181 gctggccggc ccctgccagc gcatcacctc ggcagcatcg ccgaccgcga cgaagcgccc
     3241 ggcgcggatg gcgacggcgc tcgcgtccgg gcgctcgcga tcgacggtat ggaagcggcc
     3301 gttcagcagg atcagttcgg cgccggagcc ggagtcggcg gagggagcag cggatgcgtc
     3361 ggcggacatg cgcggatacc tcggggggag cggggggaga gccgccgcgc acggtgcggc
     3421 ggcggggatt cagcgcgtca gccagccggc gagccggcgc gtcagccacg gcatcaccgc
     3481 gcagacgacg atcagcacga tgctgagcgc gacgacgaaa ttgccggcca gccagccgcc
     3541 gagccacggc cagcgcgtga acgccggctg ccagagctgc ggcacgatca gcgtcagcgg
     3601 ccagatcacc gcgaaggtca tcagcacctg cttccagagc cgcggcggct gcgcgccggc
     3661 cggcgtgaac cagaaggccg caccggggcg gatgtccagg tcctcaccgg cggcgagcag
     3721 cggccggatc tgctcgatca gctcgcgccg gtcggccgat tccatccagc ggcgcagctg
     3781 ttccaggcgc tcgaagcgca gcacgatcgt gtacggcgtg ccgggcgcat ccgggcggat
     3841 gacgctgacg ccgagatggc ccggatacgt ggccgccacc ggcatgatcc gcgtcagcca
     3901 gcgctcgtag tccgcgcgcc ggcggggttc gacgcggtgg cggatcacga agctgacgtg
     3961 gccggcgtgg gcaccgccga acaccgcggc gggcggttcg gaaggctcgg gcggctcggg
     4021 cgctgcggct gccggtgcgg cggcgttcac ttgcgcacgc tgccgcgcca ctggccgcgc
     4081 tgctgtgcgc cgtgcacgcg cgtgtaggcg tagtcgacgc ccatgccgta ggcgccgctg
     4141 tgctcgcgca cgatggccat gacctcgtcg tagtgctcgc gccgcgccca gtcgcgctgc
     4201 cattcgagca gggtctgctg ccaggtcagc ggccggatgc cggcctggat catgcgcagc
     4261 atcgagtagt cgtgcgcgtc cttgctggtg ccgccgcagg catcggcgac gacgtagacc
     4321 tcgtagccgt cgagcatcgc cgagttggcg gccgagttga tgcagacctc ggtccagagg
     4381 ccggagagga tcagcttccg gcggccgctg ccattgatcg cgtcgcgcac cttggcatcg
     4441 tcccaggaat tcatgctggt gcgctccagc agggctgcgt ccggatagac gtcgagcagc
     4501 tccggccacg tgtagccgct gaaggattcg gtctcgaccg tggtgatgaa ggtcggcacg
     4561 ccgaacacct tcgccgactt ggcgagggcg acgacgttgt tcttcagcgt ctggcgatcg
     4621 atgttggcga cgccgaaggc catctgcggc tggtgatcga tgaacagcag cacgctgttg
     4681 tcgggcgtca gctgttcgag cgcggtacgg tcggtcatgg gtcggctcct gcggagggga
     4741 tgagctttct ttcgaagacc cggccgggcg agccagcctg cgcaggtttc gggccagttc
     4801 gcacaaggtg gaaaaaaccg agtgttacac tcggaatgtg aacaggcgtt catgcatggt
     4861 tgcagcctgc gttgcagaca cgtttcatgg ttgcgctgtc atgaaacatc cgctgcgaca
     4921 tccccctgat tttcctgcgg ctgaaaccct tccccgatga ccgatccctc tcccccgaac
     4981 ggcccggcct tcgccgcgct gcgtgccgac atcgagcatc tgctgcagcg cttcacgctc
     5041 ggcgagcacg gcggacgcat gctgctgcag ggccggcgca tggtcgtgca gtcggcgcgc
     5101 tcgctcgggc agctgcgcga ggagctgatc cgcatgctcg gcgccgatct ggcgcgcggc
     5161 gtgctgaaac gctacggcta ccagtccggc ttccaggacg gtcacgcgct gcgcctgcgc
     5221 catccggagc tgccggtcga gctgcagatg cacctcggca ccgtgctgca cgagcgcgag
     5281 ggccacgcgc gcatcgagac cgagcccgag ggcacgcacg tcgatctggc gcgcggcgtg
     5341 ctcgacatcc gctcgcgctg gcacgactcg gtcgaggccg aacagcacca cctgctgctc
     5401 gggcgcagca gcgaaccggt gtgctggacg ctctccggct atgccagcgg acacctgagc
     5461 tacctgctgc agcgcgaggt cgtgatcgtc gagaccgcct gccgcgccca gggccatccg
     5521 tactgccgct ttcgcgtcgg ctttcgcgag gacatggaaa aacacttccc cggctgcgcg
     5581 gatgactatc gccgactcga cctcggccgg ctgttccgcg agctggagca gaccgtcgag
     5641 gcgcagagcc gcacgatccg cgagctgcgc gagcagctcg gcgccgcgcc ggccgccggc
     5701 gcggcggcct cggcggcgtt ctcgacgctg accggccgca gccccgcgct cgcgcgcgcc
     5761 atcgacatcg cgcgcgcggt cgccggcgtc agctcgacgg tgctggtg
//
LOCUS       DAOQJH010000059         5797 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_7920_length_5797_cov_3.680947, whole genome shotgun sequence.
ACCESSION   DAOQJH010000059 DAOQJH010000000
VERSION     DAOQJH010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 5797)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5797)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5797
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7920_length_5797_cov_3.680947"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="PLQ18_02365"
     CDS             <1..228
                     /locus_tag="PLQ18_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011398648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNE00523.1"
                     /translation="AGFDLLLAGHTHGGQVCLPGGLPLMLNADCPRAYCRGPWRYRDM
                     LGYTSTGAGSSIVGVRYNCPPEIVVHTLRRG"
     gene            complement(241..723)
                     /locus_tag="PLQ18_02370"
     CDS             complement(241..723)
                     /locus_tag="PLQ18_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015686139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-archaeol synthase"
                     /protein_id="HNE00524.1"
                     /translation="MIEALQLLFLLFVANGAPVLATRVLDTAGDHPIDGGRRFLDGRP
                     LFGPSKTWRGLGVALLASTLAALLLGWPAKIGLLIGAAAMLGDLLSSFLKRRLGLASS
                     AQALGIDQVPEALLPLLAVRETLALDGWVIAALVAAFVVLELLLSRLLYRLHIRRQPY
                     "
     gene            955..1030
                     /locus_tag="PLQ18_02375"
     tRNA            955..1030
                     /locus_tag="PLQ18_02375"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:988..990,aa:Gly,seq:gcc)
     gene            1176..1958
                     /locus_tag="PLQ18_02380"
     CDS             1176..1958
                     /locus_tag="PLQ18_02380"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNE00525.1"
                     /translation="MTDPSATPVLPAAVVHGCAAGPAVPDVLLIHGAGGDHRHFPPAL
                     TDLPGVRVHALDLPGHGAAEGRFADSVEVYADAVAAYVESRGLARVVLAGHSMGGAVV
                     MTLALRAPAWLRAQVLIGTGSRLKVLPLLFDLIDDDWPGAVATIVSGLFGPDADPAQV
                     EAVRGRYLDGPPARLRADLRACTRFDIGERLGTLRLPTLVVSGALDVMTPPKYGRALA
                     EAIPGAHHVEIAGAGHMLALERPQAVRAALAGFLTTLDHAQE"
     gene            1966..2493
                     /locus_tag="PLQ18_02385"
     CDS             1966..2493
                     /locus_tag="PLQ18_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004370831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HNE00526.1"
                     /translation="MRYRLGDRRVVCPADGDWFIADDALVIGSVTLGVEASIWYRAVV
                     RGDSEQISIGARSNVQDGAVLHADPGFPLTIGADVTVGHLAMLHGCTIGDGTLVGIQS
                     TILNGAVIGEGCLIGAHTLIPERKVIPPRSLVMGVPGRVIRELDEAAVAGLLRSAAHY
                     VANFRRHRLDLQVQD"
     gene            2601..3521
                     /locus_tag="PLQ18_02390"
     CDS             2601..3521
                     /locus_tag="PLQ18_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008931608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNE00527.1"
                     /translation="MRERLDAIIRQLGSIILGKEPALRLALACLLARGHLLIEDLPGM
                     GKTTLAQALARTLGLEYRRIQFTSDMLPADILGASVYDREQGAFVFHPGPVFAQLVLA
                     DEINRASPKTQSALLEAMEEGQVTSEGHTRRLPQPFFVIATQNPATQLGTFPLPESQL
                     DRFLLRVRLGYPDPKAERALLAGEDRRRLLDRLPVLIDPPGLLLLQARVERVHAADVL
                     LDYVQALISATRHGGQFETGLSPRAGLALLRVARAWALLAGRDHVLPEDVQVTLPALA
                     GHRLRPGAQNAGARADELLQRLVNSAPLPV"
     gene            3525..4490
                     /locus_tag="PLQ18_02395"
     CDS             3525..4490
                     /locus_tag="PLQ18_02395"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HNE00528.1"
                     /translation="MSVEPRILRWHRRRHPLLRTAHPLTRRSIYILPTRTGLVFAAVL
                     LAMLGWSINYNASLGFALVFLLGTIALEAMRRTHDNLLGLTVHPPLAEPVFAGDTAFI
                     RLRLEAPAGEIRYGVVVRTAAGVETGGDLDGPLAALDLPVPAQRRGRLASGALRVSTC
                     FPLGLFTAWTWVEFQPSGLVWPQPAGVAQLPAGTHGKPQQQPARTESAGDEDFAGLRP
                     WRRGDPATHVAWKPSARSGQLQVKQFSAPPGGDELMLDLASAPGDGIEARLSQLTLWT
                     IEADRADVRFGLRLGATELAPARGSAHRFAVLERLALYGEGADAA"
     gene            4480..>5797
                     /locus_tag="PLQ18_02400"
     CDS             4480..>5797
                     /locus_tag="PLQ18_02400"
                     /inference="COORDINATES: protein motif:HMM:NF023418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3488 and transglutaminase-like
                     domain-containing protein"
                     /protein_id="HNE00529.1"
                     /translation="MRPEAWTRPAPGARLYGLPGLALLCAMAPHVTVVPLWLAAVFIA
                     TGLWRLGAEARDWALPNRWLLAAATVALCGAIGWQYRTLFGREAGVALLYAMTSLKLL
                     EWRVHGGQRDALVLMLLGYLLVMARMLYDQGPVTALWALASVALTLAAQVRVASDGGG
                     PPVAAVARLVGGLLARAVPLALVLFVLFPRVPGPLWSLPQDAAARTGVGEDMSPGTIA
                     ELSRSDAVAFRVRFTGGLPAADRRYWRGPVLDRYDGRTWRMSEELPGQHFPQTEASQA
                     LSYVVTLEPSGRRWLYALDLPDSLPAQTGVTASGMLMRRSAVTEVVRYEQRSVLEYRT
                     GPLTNWERSRALRLPSTAAPRAQALGREWRTRFADPAERVQAALTMFRREPFRYTLSP
                     PLTPTDAVDEFLFDTRAGFCEHYAGAFVALMRAAEVPARVVTGYLGG"
BASE COUNT          736 a         2104 c         2103 g          854 t
ORIGIN      
        1 gccggcttcg acctgctgct cgccggccac acgcacggcg gccaggtctg cctgcccggc
       61 ggcctgccgc tgatgctgaa cgccgactgc ccgcgcgcct actgccgcgg tccgtggcgc
      121 taccgcgaca tgctcggcta cacctcgacc ggggccggtt cgtcgatcgt cggcgtgcgc
      181 tacaactgcc cgccggagat cgtcgtgcac acgctgcgcc ggggctgagg ccgctgcgct
      241 tcagtagggc tggcggcgga tgtgcagccg gtacagcagg cgcgacagca gcagttccag
      301 caccacgaac gccgccacca gcgcggcgat gacccagcca tcgagcgcca gggtctcgcg
      361 caccgccagc agcggcagca gggcctccgg gacctgatcg atgccgagag cctgcgcgct
      421 cgatgcaagg ccgagccggc gcttcagaaa acttgaaagg agatcgccga gcatggccgc
      481 tgcgccgatc agcaggccga tcttcgccgg ccagccgagc agcagcgcag cgagcgtgct
      541 ggccagcagc gccacgccga ggccgcgcca ggtcttgctc ggaccgaaca gcggccggcc
      601 gtcgaggaag cggcggccgc cgtcgatcgg gtgatcgccg gcggtatcga gcacgcgcgt
      661 cgccagcacc ggcgcgccgt tggcgacgaa cagcaggaac agcagctgca gggcttcgat
      721 cacgcaggct ccggtgcaca cggggacggt cgggcgatgc gccggaatgc gagtgtccgc
      781 gagcttcgct gccgatgcca gtcgtgcgct tgccatcgtt caaataaatg caacgaaacc
      841 ttcatccggg gcttgcctgc ccgggagtgg ctccgtaaga tgcgcgccgt cgtcaccgac
      901 gacacctgga cgacccctgg acgaccccgg aagggacgct ccacaacgat tcatgcggga
      961 atagctcagt tggtagagcg caaccttgcc aaggttgagg tcgcgagttc gagcctcgtt
     1021 tcccgctcca gatttcacga aaaaccccgg tcccgcgacc ggggtttttt gttgcgcacc
     1081 ggtaacgatc gcgagcgcga tcacggcccc ggcgcaggcg ggccgggctg tgctttctgc
     1141 tagcatccgg cgttccggta ccacggagcg cgctcatgac cgatccgtcc gccacaccgg
     1201 tgctgccggc cgccgtcgtc cacggttgcg ccgccggtcc agcggtcccc gacgtgctgc
     1261 tgatccacgg cgccggcggt gatcatcgcc acttcccgcc ggccctgacc gatctgcccg
     1321 gtgtccgggt gcatgcactc gacctgcccg gtcacggcgc ggccgagggg cgctttgccg
     1381 acagcgtcga ggtctacgcc gatgccgtcg ccgcctacgt cgaatcccgc gggctcgcgc
     1441 gtgtcgtgct cgcgggacac tcgatgggcg gggcggtggt gatgacgctg gcgctgcgcg
     1501 cgccggcctg gctgcgggcg caggtgctga tcggcaccgg ctcgcggctg aaggtgctgc
     1561 cgctgctgtt cgacctgatc gacgacgact ggccgggcgc ggtcgcgacc atcgtcagcg
     1621 gcctgttcgg accggatgcc gatccggcgc aggtcgaggc cgtgcgcggg cgctatctcg
     1681 acggcccgcc ggcgcggctg cgcgccgacc tgcgggcctg cacccgcttc gatatcggcg
     1741 agcggctcgg cacgctgcgc ctgccgacgc tggtggtctc gggcgcgctc gatgtcatga
     1801 cgccgccgaa gtacggccgc gcgctcgccg aggcgatccc gggcgcgcac cacgtcgaga
     1861 tcgccggggc cgggcacatg ctcgcgctcg aacggccgca ggcggtgcgc gccgccctcg
     1921 ccggattcct caccacgctc gatcacgctc aggagtgatg ccgccatgcg ctaccggctc
     1981 ggggatcgtc gcgtcgtctg tcccgcggac ggcgactggt tcatcgccga tgacgcactg
     2041 gtcatcggct cggtgacgct cggcgtcgag gcgagcatct ggtatcgcgc cgtcgtgcgc
     2101 ggcgacagcg agcagatcag catcggcgcg cgctcgaacg tgcaggatgg cgccgtgctg
     2161 cacgccgatc cgggctttcc gctgacgatc ggggccgacg tgacggtcgg gcatctggcg
     2221 atgctgcacg gctgcacgat cggcgacggc acgctggtcg gcatccagag cacgatcctc
     2281 aacggcgccg tgatcggcga gggctgcctg atcggcgcgc acacgctgat ccccgagcgc
     2341 aaggtgatcc cgccgcgctc gctggtcatg ggcgtgccgg ggcgcgtgat ccgggaactg
     2401 gacgaggccg ccgtggccgg attgctgcgc tccgccgcgc attacgtcgc caatttccgc
     2461 cgccaccgcc tggacctgca ggtccaggac tgaaagcgcc cgccccacgg gcgcacgcga
     2521 tcaggagaac catggaaaga accttggaag ccgtgcaggg aaccgctgcc gcgccggcaa
     2581 ctgcgatcag tacggccgcg ctgcgcgagc ggctcgatgc catcatccgc cagctcggca
     2641 gcatcatcct cggcaaggag ccggcgctgc gcctggcgct cgcctgcctg ctcgcgcgcg
     2701 gccacctgct gatcgaggac ctgcccggca tgggcaagac cacgctggcg caggcgctgg
     2761 cgcgcacgct cgggctcgaa taccggcgca tccagttcac cagcgacatg ctgccggccg
     2821 acatcctcgg cgcttcggtc tacgaccgcg agcagggcgc cttcgtgttc catccggggc
     2881 cggtgttcgc gcagctcgtg ctcgccgacg agatcaaccg cgcctcgccg aagacgcaga
     2941 gcgcgctgct cgaagcgatg gaggaaggtc aggtcaccag cgagggtcac acgcggcggc
     3001 tgccgcagcc gttcttcgtg atcgcgacgc agaacccggc gacccagctc ggcaccttcc
     3061 cgctgccgga atcgcagctc gatcgcttcc tgctgcgcgt gcgcctcggc tacccggatc
     3121 cgaaggccga gcgcgcgctg ctcgccggcg aggaccgccg ccgcctgctc gaccggctgc
     3181 cggtgctgat cgatccgccc gggctgctgc tgctgcaggc gcgcgtcgag cgcgtgcatg
     3241 ccgccgacgt gctgctcgat tacgtgcagg cgctgatctc ggcgacccgc cacggcggcc
     3301 agttcgagac cggcctgtcg ccgcgcgccg ggctggcgct gctgcgggtc gcgcgtgcct
     3361 gggcgctgct cgccgggcgg gatcacgtgc tgcccgagga cgtgcaggtg acgctgccgg
     3421 cgctcgccgg gcatcgcctg cgccccggcg cgcagaacgc cggcgcgcgg gccgacgagc
     3481 tgctgcagcg cctggtcaac tcggcgccgc tgccggtctg atcgatgagc gtcgaaccgc
     3541 gcatcctgcg ctggcaccgt cgccggcatc cgctgctgcg caccgcgcat ccgctgacgc
     3601 ggcgttcgat ctacatcctg ccgacgcgca ccggccttgt gttcgccgcg gtgctgctgg
     3661 cgatgctcgg ctggtcgatc aactacaacg ccagcctcgg cttcgcgctg gtgttcctgc
     3721 tcggcacgat cgcgctcgag gcgatgcgcc gcacgcacga caacctgctc gggctgaccg
     3781 tgcatccgcc gctggccgag ccggtgttcg ccggcgacac cgcgttcatc cgcctgcgtc
     3841 tggaggcccc ggccggcgaa atccgctacg gcgtggtcgt gcgcaccgcc gccggcgtcg
     3901 agaccggcgg cgatctcgac gggccgctgg ccgcgctcga cctgccggtg ccggcgcagc
     3961 gccgcgggcg gctcgcgagc ggggcgctgc gcgtgtcgac ctgctttccg ctcggactgt
     4021 tcacggcctg gacctgggtc gagttccagc cttccgggct ggtctggccg cagccggccg
     4081 gcgtggcgca gctgccggcc ggcacgcacg gcaagccgca gcagcagccg gcgcgcaccg
     4141 agagcgccgg tgacgaggac ttcgccggcc tgcggccctg gcggcgcggt gatccggcca
     4201 cgcacgtcgc gtggaagccg tcggcgcgca gcggccagct gcaggtcaag cagttcagcg
     4261 cgccgcccgg cggcgacgag ctgatgctcg atctggcgag cgcgcccggc gacggcatcg
     4321 aggcgcggct gtcgcagctg acgctgtgga cgatcgaggc cgaccgggcc gatgtgcgct
     4381 tcggcctgcg cctcggcgcc acggaactcg cgccggcgcg cggcagcgcg caccgcttcg
     4441 cggtgctgga gcgcctggcg ctgtacgggg agggtgccga tgcggcctga agcctggacc
     4501 cgcccggcac ccggtgcccg gctctacggc ctgccggggc tcgcgctgct ctgcgcgatg
     4561 gcgccgcatg tgacggtcgt gccgctgtgg ctggcggcgg tgttcatcgc caccggcctc
     4621 tggcggctcg gcgccgaggc acgcgactgg gcgctgccga accgctggct gctggcggcg
     4681 gcgaccgtcg cgctgtgcgg cgcgatcggc tggcagtacc gcacgctgtt cgggcgcgag
     4741 gccggcgtcg cgctgctgta cgcgatgacc tcgctgaagc tgctcgaatg gcgcgtgcac
     4801 ggcgggcagc gcgatgcgct ggtgctgatg ctgctcggct atctgctggt catggcgcgc
     4861 atgctctacg accagggccc ggtgacggcg ctgtgggcgc tggcgagcgt cgcgctgacg
     4921 ctggctgcgc aggtgcgcgt cgcgagcgac ggcggcggac cgccggtggc ggccgtcgcg
     4981 cggctggtcg gcggtctgct cgcgcgcgcc gtaccgctgg cgctggtgct gttcgtgctg
     5041 ttcccgcgcg tgccggggcc gctgtggagc ctgccgcagg atgctgccgc ccgcaccggc
     5101 gtcggcgagg acatgtcacc gggcacgatc gccgagctga gccgctccga tgccgtcgcc
     5161 ttccgcgtgc gcttcaccgg cggcctgccg gctgcggatc gtcgctactg gcgcgggccg
     5221 gtgctcgatc gctacgacgg tcgcacctgg cgcatgagcg aggaactgcc gggccagcat
     5281 tttccgcaga ccgaagccag ccaggcgctg agctacgtgg tcacgctcga accgagcggc
     5341 cggcgctggc tgtacgcgct cgacctgccg gactcgctgc cggcgcagac cggcgtcacc
     5401 gcctccggca tgctgatgcg gcgcagcgcc gtgaccgagg tcgtgcgcta cgagcagcgc
     5461 tcggtgctcg aataccgcac cgggccgctg acgaactggg agcgcagccg cgcgctgcgc
     5521 ctgccgtcga cggccgcgcc gcgcgcgcag gccctggggc gcgagtggcg cacgcgtttc
     5581 gccgatccgg ccgagcgcgt gcaggcggcg ctgacgatgt tccggcgcga gcccttccgc
     5641 tacacgctgt cgccgccgct gacgccgacc gatgcagtcg atgaattcct gttcgatacc
     5701 cgcgccggct tctgcgaaca ctacgccggc gccttcgtgg cgctgatgcg tgccgccgag
     5761 gtgccggcgc gcgtggtcac cggctatctc ggcggtg
//
LOCUS       DAOQJH010000060         5772 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_7973_length_5772_cov_4.593318, whole genome shotgun sequence.
ACCESSION   DAOQJH010000060 DAOQJH010000000
VERSION     DAOQJH010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 5772)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5772)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5772
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7973_length_5772_cov_4.593318"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            13..150
                     /locus_tag="PLQ18_02405"
     CDS             13..150
                     /locus_tag="PLQ18_02405"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNE00530.1"
                     /translation="MVCCQHGNRSAPLAAKLKKAGFTSVMNLAGGVAAWTGAGLPLVR
                     K"
     gene            147..431
                     /gene="grxC"
                     /locus_tag="PLQ18_02410"
     CDS             147..431
                     /gene="grxC"
                     /locus_tag="PLQ18_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HNE00531.1"
                     /translation="MNAPARRPEIIVYASRWCPYCRAAFALLDRKGVAYEVLDVDEQP
                     ALWNEIDARTGRDTVPQTFIDGQHVGGYDDMAALDRAGGLDPLLFPKAPG"
     gene            578..1069
                     /gene="secB"
                     /locus_tag="PLQ18_02415"
     CDS             578..1069
                     /gene="secB"
                     /locus_tag="PLQ18_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011867598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051262 - protein tetramerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HNE00532.1"
                     /translation="MSEQNSAPQQPQQHFEIQKLYTKDISFETPNSPMIFTEQWTGES
                     QLQLHNESAQLADNIHEVVLTVTVTTKQGDKTAYLCEVRQAGIFTIAGFAPDQLAAML
                     GAYCPNVLFPYAREAVSNLIQRGGFPPLQLAPVNFDALYMQHLQAKQAQQMQAGQPSS
                     GTA"
     gene            1113..2108
                     /locus_tag="PLQ18_02420"
     CDS             1113..2108
                     /locus_tag="PLQ18_02420"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014779142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HNE00533.1"
                     /translation="MATLAVLGAGSWGTALALLLARNGHAVRLWGHDPAQVARLATER
                     ENAAFLPGVTLPGRITPGADLAAACAGAAMVLVVVPSHAFVATLDALAALAQPPAALA
                     WATKGLEPRTGRFLHEVARERLGSRPLAVVSGPNFAREVARGLPTATTVASLDAGWAA
                     TVAGFLHGRDFRAYTVTDVSGVELGGAVKNVLAIACGVADGLGYGANARAALITRGLA
                     EMVRLGLACGGRRETFMGLAGVGDLVLTCTDDQSRNRRFGLALGRGAGVGEALAGIGQ
                     VVEGAAAVAEVLKLARSHGVEMPISEQVEAVVHGGRPPAAAVEALLAREPRDDGV"
     gene            2118..2561
                     /locus_tag="PLQ18_02425"
     CDS             2118..2561
                     /locus_tag="PLQ18_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004749113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HNE00534.1"
                     /translation="MYGWLLLLHILGASVWTGGHLVLSLVILPGVLRERSAAKLLAFE
                     SGFEKIGMPALVVQVVTGLLLAWRQLPQLTAWFDPANPLAHAILAKLGLLLATFLLAL
                     DAKLRVLPHFDDSRLTDMAWHIVPVTVLSVLFVAAGVSFRTGWLY"
     gene            complement(2628..3512)
                     /locus_tag="PLQ18_02430"
     CDS             complement(2628..3512)
                     /locus_tag="PLQ18_02430"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HNE00535.1"
                     /translation="MRPDTLRKHTHSEPSARSHALPRNSRKARHLALAGLGGLLAAMV
                     LLAPKPVRAESGKLSIDRRCVLPAQMVRLDGALERTRARIDRREPLTIVAIGSSSTEG
                     YGASSPANTYPAQLGSLLRTRYVGEPIEVINKGRGGEVATQTLARFGKDDYPNDPTLV
                     IWQAGANDVVRNVPLEQFAATLKEGLAALRSAGIDVILMPPQYAPQTAAAKQIEAYVE
                     LIEQLGDEFDVPVVQRYELMQQLAAKDAPLLAAMLTSDGLHQNDLGYHCLAAQVAMGI
                     DTLDAGSTGGTLATTLAP"
     gene            complement(3553..4353)
                     /locus_tag="PLQ18_02435"
     CDS             complement(3553..4353)
                     /locus_tag="PLQ18_02435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE00536.1"
                     /translation="MTPPRCRRPVLFLLAALLGAGVHAEVVPPVPSADEDDDSPVAPE
                     VSECGDPSLIRGTPFPLSHVAAARLENRDVTILVLGTASTAGRAGPTSPTLSYPVRME
                     HWLEARWGDRRVHVVNLARPGQTAAQQIEKLPEALATYHPELLVWQTGTVDAIRGVDI
                     NEFGDAIEHGLQYAENAAADTLLIDAQYGSEAFVLYDAGPYLGYLRQIAMGRRAALFP
                     RFEIMRRWVEQGLLDLDPADAESQRRVADRIHDCLGRYLADMIDRASR"
     gene            complement(4350..5489)
                     /locus_tag="PLQ18_02440"
     CDS             complement(4350..5489)
                     /locus_tag="PLQ18_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013504589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OpgC domain-containing protein"
                     /protein_id="HNE00537.1"
                     /translation="MPALPRPAGRDARLDFFRGLALIYIFLDHIPSNVVAWITVRNYG
                     FCDATEIFVFIAGYSATLAYGSQMRRHGFLYGSASLLQRAWQLYIAHIFLFVLFTAQI
                     GWVETRFRNPMYSEEMNLLGFLSEPHVNLIQALLLRFRPTNMDVLPLYIALLLGFAPL
                     LWLLLHRQWLVLGLSGLLYLGAGANNWNLAGYPPGRTWFFNPLCWQFLFVIGACCALS
                     PAAMAVLTRQRRWLQPLCIAYLLFALAVVGTWYWPALETWLPAWLARLIYPIDKTNLD
                     ILRLLHFLALAYLVTQRVGPDAAFLHGRLARPFVICGRHSLEIFCLGTLLAFTGHFYL
                     TEFHGALPAQIAVSVAGTLLMILVAWIMEWYRNTGTQGRPSGASA"
BASE COUNT          864 a         2042 c         2072 g          794 t
ORIGIN      
        1 gaccgcgaac tcatcgtctg ctgccagcac ggcaaccgct cggcaccgct ggcggcgaag
       61 ctgaagaagg ccggcttcac gtcggtcatg aacctcgccg gcggcgtcgc cgcgtggacc
      121 ggcgccggcc tgccgctggt gcgcaagtga acgcgccggc gcgccggccc gagatcatcg
      181 tctacgcctc gcgctggtgc ccgtactgcc gcgcggcctt cgcgctgctc gatcgcaagg
      241 gcgtcgccta cgaagtgctc gatgtcgacg agcagccggc gctgtggaac gagatcgacg
      301 cgcgcaccgg ccgcgacacc gtgccgcaga ccttcatcga cggccagcac gtcggcggct
      361 acgacgacat ggccgcactc gatcgcgccg gcggcctcga cccgctgctg ttcccgaagg
      421 cccccggctg aaagcaagcc cccgcccgca cgccggcgca cgtgcttggc cgtaccgcag
      481 cgcgcgcgta taaagcctga cccgcccggg cgcacgccgt ccgcgccgcc agcaaccctc
      541 aagaccctcc aagaccacgg tccggaagcc gacacccatg agcgagcaga acagcgcccc
      601 gcagcagccg cagcagcact tcgagatcca gaagctgtac acgaaggaca tctccttcga
      661 gaccccgaac tcgccgatga tcttcaccga gcagtggacc ggcgagagcc agctgcagct
      721 gcacaacgaa tccgcgcagc tcgccgacaa catccacgag gtcgtgctga ccgtcaccgt
      781 caccaccaag cagggcgaca agaccgccta cctgtgcgaa gtccgccagg ccggcatctt
      841 cacgatcgcc ggcttcgcac ccgaccagct cgcggcgatg ctcggtgcct actgcccgaa
      901 cgtgctgttc ccgtacgcgc gcgaggcggt ctcgaacctg atccagcgcg gcggcttccc
      961 gccgctgcag ctcgcgccgg tgaacttcga cgcgctgtac atgcagcacc tgcaggccaa
     1021 gcaggcgcag cagatgcagg ccggccagcc gagcagcggc acggcctgac cggcctgctc
     1081 cggctgaccc tgccccgccc gcccggcacc tgatggccac gctcgcggta ctcggcgccg
     1141 gctcctgggg cacggcgctc gcgctgctgc tggcgcgcaa cggtcatgcc gtgcgcctgt
     1201 ggggccacga tccggcgcag gtcgcacggc tcgccaccga acgcgagaac gccgccttcc
     1261 tgcccggcgt gacgctgccc gggcgcatca cgccgggcgc cgatctcgcc gccgcctgcg
     1321 ccggcgcggc gatggtgctg gtggtcgtgc cgagccacgc cttcgtcgcg acgctcgatg
     1381 cgctcgccgc gctggcgcag ccgccggcgg cgctcgcctg ggccaccaag gggctggagc
     1441 cgcgcaccgg acgcttcctg cacgaggtcg cgcgcgagcg cctcggcagc cggccgctgg
     1501 cggtggtctc cggcccgaac ttcgcgcgcg aggtcgcgcg cggcctgccg accgcgacca
     1561 cggtcgcgag cctcgacgcc ggctgggcgg cgacggtcgc cggcttcctg cacgggcgcg
     1621 acttccgcgc ctacaccgtc accgacgtga gcggcgtcga actcggcggc gcggtcaaga
     1681 acgtgctcgc gatcgcctgc ggcgtcgccg acggcctcgg ctacggcgcc aacgcccgcg
     1741 cggcgctgat cacgcgcggt ctggccgaga tggtgcgcct cggcctcgcc tgcggcggcc
     1801 ggcgcgagac cttcatgggg ctcgccggcg tcggcgatct ggtgctcacc tgcaccgatg
     1861 accagtcgcg caaccgccgc ttcggcctcg cgctcgggcg tggggccggc gtcggggagg
     1921 cactcgccgg catcggtcag gtcgtcgagg gcgcggccgc ggtcgccgaa gtcctgaagc
     1981 tcgcgcgcag ccacggcgtc gagatgccga tctccgagca ggtcgaggcc gtcgtccacg
     2041 gcggccggcc accggcggcg gcggtcgagg ccctgctggc gcgcgagccg cgcgatgacg
     2101 gggtgtgagg acggcccatg tacggatggc tgctgctgct gcacatcctc ggtgccagcg
     2161 tctggaccgg cgggcacctc gtgctgtcgc tcgtgatcct gcccggtgtg ctgcgcgagc
     2221 gctcggcagc gaagctgctc gcgttcgaat cgggcttcga gaagatcggc atgccggcgc
     2281 tggtcgtgca ggtcgtcacc ggcctgctgc tcgcctggcg gcagctgccg cagctgacgg
     2341 cgtggttcga tccggccaat ccgctcgcgc acgcgatcct tgccaagctc ggactgctgc
     2401 tcgcgacctt cctgctcgcg ctcgatgcca aactgcgcgt gctgccgcac ttcgatgatt
     2461 cgcggctcac cgacatggcc tggcacatcg tgccggtgac ggtgctgtcg gtgctgttcg
     2521 tcgcggccgg cgtgtcgttt cgcaccggct ggctgtactg aaacgcctgc accggcaatg
     2581 cgcccgcccc tgccgcgcac cgcctcgcgg cagggacgcg cgccggctta cggcgccagc
     2641 gtcgtcgcca gcgtgccgcc ggtgctgccg gcatcgagcg tgtcgatgcc catcgccacc
     2701 tgcgcggcga ggcagtggta gccgaggtca ttctgatgca ggccgtcgga ggtcagcatc
     2761 gccgccaaca gcggggcatc cttcgcggcg agctgctgca tcagttcgta gcgctgcacg
     2821 accggcacgt cgaactcgtc gccgagctgc tcgatcagct cgacataggc ctcgatctgc
     2881 ttcgcggctg cggtctgcgg cgcgtactgc ggcggcatca ggatcacgtc gatgccggca
     2941 ctgcgcagcg ccgccagacc ttccttgagc gtcgcggcaa actgttcgag cgggacgttg
     3001 cgcaccacgt cattggcgcc ggcctgccag atcaccagcg tcgggtcgtt cggatagtca
     3061 tccttgccga agcgcgccag cgtctgcgtg gccacctcgc cgccgcggcc cttgttgatg
     3121 acctcgatcg gctcgccgac gtagcgcgtg cgcagcaggc tgccgagctg ggccggatag
     3181 gtgttggccg gcgagctggc gccgtagcct tcggtcgacg aggagccgat cgcgacgatg
     3241 gtcagcggct cgcgccggtc gatccgggcg cgcgtgcgct ccagcgcgcc gtcgaggcgc
     3301 accatctgcg ccggcagcac gcagcggcgg tcgatcgaca gcttgccgct ttcggcacgc
     3361 accggcttcg gggcgagcag caccatcgcc gccagcaggc cgccgaggcc ggcgagcgcc
     3421 agatgccggg ccttgcggct gttgcgcggc agcgcgtggc tgcgcgcgct cggctcggag
     3481 tgggtgtgct tgcgcagggt atcgggtcgc atcgggtgtc tccctgttat ggcgctgcgc
     3541 cttcaccggc gctcagcgtg aagcccggtc gatcatgtcg gccaggtacc ggccgagaca
     3601 gtcgtggatg cgatcggcaa cccggcgctg tgactcggcg tccgccggat cgaggtcgag
     3661 caagccctgc tcgacccacc ggcgcatgat ttcgaaacgc gggaacagcg cggcgcgccg
     3721 tcccatcgcg atctggcgaa ggtaacccag ataagggccg gcatcataga gaacaaaggc
     3781 ttcgctgccg tactgcgcgt cgatcagcag cgtgtcggcc gcggcgttct cggcgtactg
     3841 caggccgtgc tcgatggcat cgccgaactc gttgatgtcg acgccgcgga tcgcatcgac
     3901 ggtgccggtc tgccagacca gcagttcggg gtgataggtc gcgagcgcct cgggcagctt
     3961 ctcgatctgc tgggcggcgg tctggccggg gcgggcgagg ttgacgacgt gcacgcgcct
     4021 gtcgccccag cgcgcctcca gccagtgctc catgcgcacc ggatagctca gcgtcggcga
     4081 ggtcgggccg gcacgcccgg cggtcgaggc cgtgccgagc acgaggatgg tcacgtcgcg
     4141 gttctcaagc cgcgccgcgg cgacgtgcga cagcgggaac ggcgtgccgc ggatcagtga
     4201 cggatcgccg cattcgctga cttcgggcgc gaccggggaa tcatcgtcct cgtccgccga
     4261 cgggacgggc ggcacgacct cggcatgcac tccggcaccg agcagcgcgg cgagcaggaa
     4321 cagcacggga cggcggcagc gggggggagt catgccgaag ctccggaggg acggccctgg
     4381 gtgccggtgt tgcggtacca ctccatgatc caggcgacga ggatcatcag cagcgtgccg
     4441 gcgacgctga cggcgatctg cgccggcagc gcaccgtgga attcggtcag gtagaagtgg
     4501 ccggtgaacg ccagcagcgt gccgaggcag aagatctcca gcgaatgccg gccgcagatc
     4561 acgaacggcc gcgccagccg gccgtgcagg aacgccgcat ccgggccgac gcgctgggtc
     4621 acgaggtatg cgagcgccag aaagtgcagc aggcgcagga tgtcgaggtt ggtcttgtcg
     4681 atcgggtaga tcagccgcgc cagccaggcc ggcagccagg tctccagcgc cggccagtac
     4741 caggtgccga ccaccgccag cgcgaacagc agataggcga tgcacagcgg ctgcagccag
     4801 cggcgctgtc gcgtcagcac cgccatcgcc gccggactca gcgcgcagca ggccccgatc
     4861 acgaacagga actgccagca gagcggattg aagaaccagg tccgcccggg cggatagccg
     4921 gcgaggttcc agttgttggc accggcgccg aggtacagca gccccgacag tccgagcacc
     4981 agccactggc ggtgcagcag cagccacagc agcggcgcga agccgagcag cagcgcgatg
     5041 tacagcggca gcacgtccat gttggtcggc cggaagcgca gcagcagcgc ctggatcagg
     5101 ttcacgtgcg gttcgctgag gaagccgagc aggttcattt cctccgagta catcggattg
     5161 cggaagcgcg tttccaccca gccgatctgc gccgtgaaca gcacgaacag gaagatgtgc
     5221 gcgatgtaca gctgccaggc gcgctgcagc aggctggcgc tgccgtacag gaagccgtgg
     5281 cggcgcatct ggctgccgta ggcgagcgtc gcgctgtagc cggcgatgaa cacgaagatc
     5341 tcggtcgcat cgcagaagcc gtagttgcgg accgtgatcc acgcgaccac gttcgacggg
     5401 atgtgatcga ggaagatgta gatcagcgcc agtccgcgga agaaatccag ccgggcgtcg
     5461 cgaccggcgg ggcggggcaa ggccggcacc ggcgggacgg ccggcagcac cgcttccggg
     5521 ggcgcggccg gctccgtcac cggcgccggg ggcggcgctt ccgcgggctc ggctcgcgac
     5581 ggcaccggag caggcccggc ggtgacgggg gggcgcttca ggacacgcag agcgctgggc
     5641 aaggcggcgg tctccggccg ggcgccgaca ggcagcgcgg tgcgcgcaga gtaggccggg
     5701 ccgccggcag ccgacagggc tgcgccggga gtggatcagg aaaggcggcc ggagcgcgac
     5761 gcagccggcg gg
//
LOCUS       DAOQJH010000061         5713 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_8099_length_5713_cov_4.065217, whole genome shotgun sequence.
ACCESSION   DAOQJH010000061 DAOQJH010000000
VERSION     DAOQJH010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 5713)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5713)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5713
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8099_length_5713_cov_4.065217"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1559)
                     /gene="rapA"
                     /locus_tag="PLQ18_02445"
     CDS             complement(<1..1559)
                     /gene="rapA"
                     /locus_tag="PLQ18_02445"
                     /inference="COORDINATES: protein motif:HMM:NF003426.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HNE00538.1"
                     /translation="MNDFVGGSRWISETEPELGLGMVLSGDARRVTLLFHGSGETRVY
                     ARQGAPLTRVRFQPGDTVQSHEGWTLQVEEVLEERGLFAYVGRDGRVLPETGLSNFIS
                     FSKPQERLFANQTDAQRLFTLRCDALAWAAQARASELRGLAGARIDLLAHQLWIAHEV
                     AGRYAPRVLLADEVGLGKTVEAGLILHRQLVTGRAGRALIVVPEPLIHQWFLELRRRF
                     QLGCAVYDEERCLELEREGLDNPFLAEQLVLCGRDWLVGRERRQTQALEAGWDLLIVD
                     EAHHLAWEPGAPSPEYTAIERLAAHAQGLLLLTATPEQLGPNGHYARLRLLDPERFPD
                     LDRFRHEALAYRKVARIVDDLLAYRPLDQADLDELGACLGPETGDLMLERLSLPGTDG
                     DAARARLIVELLDRHGTGRVLFRNTRAAMPNFPRRVPLPLALDCPPAYRDGGGDGVGL
                     APERAYRPGADAPAWWRFDPRVDALIELLAAGDKVLLICAHKATVLELDEALRVKSGV
                     PAALFHEDMPLL"
     gene            1709..2617
                     /locus_tag="PLQ18_02450"
     CDS             1709..2617
                     /locus_tag="PLQ18_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HNE00539.1"
                     /translation="MRLTLDALAVLDAIARRGSFAAAADELHRVPSAITYTVHKLEQD
                     LDVQVFDRSGHRARLTPVGETLLAEGRHLLRAAGELECLVRRVATGWEAELSIALSAL
                     LPLTPLLPLVEAFDRETPGTRLRLGTEVLGGVWDALVTGRADLAIGASGDGPVGGGYA
                     SRALGTIGFVFVCAPQHPLARSEVPLGEETILASRAIAVADSSRVLAPRTAALLSGQP
                     VLTVPSMEAKIDAHRAGLGVGYVPRAWVREDLAAGRLCECAVEHPLPPAPLYLAWRTG
                     NGGRGLKWFLRALEELAPWRDWLDAE"
     gene            2705..4141
                     /locus_tag="PLQ18_02455"
     CDS             2705..4141
                     /locus_tag="PLQ18_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010090145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNE00540.1"
                     /translation="MASVQRLPAFFDHAERIRREAARYLFDYFEACCEGAMAVDREAR
                     IVWINEPYAQALGLADAAAALGKPVEDVIPHSLMREVVQSGRPILLDIMQHGARSLVV
                     TRLPLTDEHGAVIGAVGFVLYDDPRSLQPLMTKFSRLRAELDAARRELAASRRARYRL
                     DDYVGSSPAALEVKRQAARAAASDSTVLLLGETGTGKELIAQAIHAASARAARPFVGI
                     NVAAVPDGLLEAEFFGSAPGAYTGADRRGRDGKFKLADGGTLFLDEIGDMPAHLQAKL
                     LRVLQEQEFEPLGSNRVERIDVRVIAATSRDLPAQVAGGRFRADLWFRLNVLPIRLPP
                     LRERRDDLPLLAGALLDRLSARAGGVRRVLGASALAELAGYDWPGNTRELGNVLERAV
                     MLSDRRCYEAADLAGLLRPPAAAAASAPAAPAVQRLADAVAEAERTAIRSALAVSGGR
                     KVDAAKLLGISRATLYEKLALLGLQGTG"
     gene            4402..5142
                     /gene="phbB"
                     /locus_tag="PLQ18_02460"
     CDS             4402..5142
                     /gene="phbB"
                     /locus_tag="PLQ18_02460"
                     /EC_number="1.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007038941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018454 - acetoacetyl-CoA reductase
                     activity [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetyl-CoA reductase"
                     /protein_id="HNE00541.1"
                     /translation="MGKVAIVTGGIGGLGTAMCKALCAQGRTVVAAYYPAEADKAAEW
                     QKARKEEGLDIAVYPVDVSDLESCAQLVAKVEADLGPVEILVNNAGITRDSVFKRMTD
                     AQWLAVINTNLNSLFNMTKPVFEKMTSRGWGRIINISSVNGQKGQFGQANYSAAKAGA
                     HGFTMALAQEGAKAGVTVNTISPGYIGTDMVMAIAEEVRNKIIATIPVGRLGTPEDIA
                     RTVAFLADDEAGFITGADMYVNGGLYIH"
     gene            5481..>5713
                     /locus_tag="PLQ18_02465"
     CDS             5481..>5713
                     /locus_tag="PLQ18_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012700027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HNE00542.1"
                     /translation="MLGILGIVLSLALLIFLAYRGWNVIFIAPFCATVALLFNWGDAP
                     LLATYTQTFMPALGNYFAKFFPLFLLGAVFGKL"
BASE COUNT          825 a         2031 c         1996 g          861 t
ORIGIN      
        1 agcagcggca tgtcctcgtg aaacagcgcc gccggcacgc cgctcttcac gcgcagcgcc
       61 tcgtcgagtt cgagcacggt cgccttgtgc gcgcagatca gcagcacctt gtcgccggcc
      121 gcgagcagct cgatcagcgc atcgacgcgc ggatcgaaac gccaccaggc cggcgcatcg
      181 gccccgggtc ggtacgcccg ctcgggggca agcccgactc cgtcgccgcc accgtcgcgg
      241 taggcgggcg ggcagtcgag cgcgagcggc agcggcacgc ggcgcgggaa gttcggcatc
      301 gccgcgcgcg tgttgcgaaa cagcacgcgg ccggtgccgt ggcgatcgag cagctcgacg
      361 atcagccgcg cgcgtgcggc gtcgccgtcg gtcccgggca gcgacaggcg ttcgagcatc
      421 agatcaccgg tctcgggccc gagacaggcg ccgagttcat cgagatcggc ctggtcgagc
      481 ggccggtagg cgagcaggtc atcgacgatg cgcgcgacct tgcggtaggc gagtgcctcg
      541 tgccggaagc ggtcgaggtc cgggaagcgc tcggggtcga gcaggcgcag gcgcgcgtag
      601 tggccgttcg gaccgagctg ctcgggggtg gcggtcagca gcagcaggcc ttgtgcatgt
      661 gcggcgaggc gttcgatcgc cgtgtactcg gggctcgggg cgcccggttc ccaggcgagg
      721 tgatgcgcct cgtcgacgat cagcaggtcc cagccggctt cgagtgcctg cgtctgacgg
      781 cgctcgcgcc cgaccagcca gtcgcggccg cagagcacca gctgttcggc caggaaggga
      841 ttgtcgaggc cctcgcgttc gagttcgagg cagcgttcct cgtcgtagac ggcgcagccg
      901 agctggaagc gccggcgcag ttcgaggaac cactggtgga tcagcggttc gggcacgacg
      961 atcagcgccc ggccggcgcg cccggtcacg agctggcggt gcaggatcag accggcctcg
     1021 acggtcttgc cgaggccgac ctcgtcggcg agcagcacgc gcggtgcgta gcggccggcg
     1081 acctcgtgcg cgatccagag ctggtgcgcg agcaggtcga tgcgcgcacc ggcgaggccg
     1141 cgcagctccg aggcgcgcgc ctgcgcggcc caggccagcg cgtcgcagcg cagcgtgaac
     1201 aggcgctggg cgtcggtctg gttggcgaac agccgctcct gcggcttgct gaagctgatg
     1261 aagttcgaca gcccggtctc gggcagcacg cggccgtcgc gcccgacgta ggcgaacagc
     1321 ccgcgctctt cgagcacctc ctcgacctgc agcgtccagc cctcgtggct ctgcaccgta
     1381 tccccgggct ggaagcgcac gcgcgtcagc ggcgcgccct ggcgcgcgta gacgcgggtt
     1441 tcgccgctgc cgtggaacag cagcgtcacg cgccgggcat cgcccgacag caccatgccc
     1501 aggcccagtt ccggttcggt ttcgctgatc cagcggctgc cgccgacgaa gtcgttcaag
     1561 tccgctcact cctggcgcat gaaaaggcgg cggatgataa cgccgtgctg cccgtgactc
     1621 gaccgccggc cggttagcga ctacatcaga gggcttgccc agccgcagca ttgcgccacc
     1681 ccgcctgccg catgccggag tccgtccgat gcgcctgacc ctcgatgccc tcgccgtgct
     1741 cgatgccatc gcccgccgcg gcagcttcgc ggccgctgcc gatgagctgc accgcgtgcc
     1801 ctcggcgatc acctacaccg tgcacaagct cgaacaggat ctcgacgtgc aggtgttcga
     1861 ccgcagcggc caccgcgcgc gcctgacccc ggtcggcgaa accctgctcg ccgaggggcg
     1921 tcacctgctg cgcgccgccg gcgagctcga atgcctggtg cggcgcgtcg ccaccggctg
     1981 ggaggcggag ctgtcgatcg cgctcagcgc gctgctgccg ctcacgccgc tgctgccgct
     2041 ggtcgaggcc ttcgatcgcg agacccccgg cacgcgcctg cgcctgggca ccgaggtgct
     2101 cggcggcgtc tgggatgcgc tggtcaccgg ccgcgccgat ctggcgatcg gcgcgagcgg
     2161 cgacggtccg gtcggcggcg gctacgcgag ccgtgccctc ggcacgatcg gcttcgtgtt
     2221 cgtgtgcgcg ccgcagcatc cgctggcccg cagcgaggtg ccgctcggcg aggagaccat
     2281 cctggccagc cgcgcgatcg ccgtcgccga cagctcgcgg gtgctggccc cgcgcacggc
     2341 ggcgctgctc agcggccagc cggtgctgac cgtgccgagc atggaggcca agatcgacgc
     2401 gcatcgcgcc gggctcggcg tcggctacgt gccgcgcgcc tgggtgcgcg aggacctcgc
     2461 cgccggccgg ctgtgcgagt gtgcggtcga gcatccgctg ccgccggcac cgctgtatct
     2521 cgcctggcgc accggtaacg gcggacgcgg cctgaagtgg ttcctgcgcg cgctggagga
     2581 actcgcgccg tggcgcgact ggctcgatgc cgaatgatgg ccgtggccgg acgcgctaac
     2641 ctcaagctcc cgtccgcccc ttcatgctgt ggtggaatct gccgtcctgc cgggagccac
     2701 ggtcttggcc agcgtccagc gcctgcccgc cttcttcgat cacgccgagc gcatccgccg
     2761 cgaggccgcg cgttacctgt tcgattactt cgaggcctgc tgcgaaggcg cgatggcggt
     2821 cgatcgcgag gcgcgcatcg tctggatcaa tgagccctac gcgcaggcgc tcgggctcgc
     2881 ggacgcggcc gccgcgctcg gcaagccggt cgaggatgtc atcccgcaca gcctgatgcg
     2941 cgaggtcgtg cagagcggcc ggccgatcct gctcgacatc atgcagcacg gtgcgcgctc
     3001 gctggtggtc acgcgcctgc cgctgaccga cgagcacggc gccgtcatcg gcgcggtcgg
     3061 cttcgtgctc tacgacgatc cgcgctcgct gcagccgctg atgaccaagt tctcgcgcct
     3121 gcgcgccgag ctcgacgccg cccggcgcga gctcgccgcc agccgccgcg cccgctaccg
     3181 gctcgatgac tacgtcggca gcagcccggc ggcgctggag gtcaagcgcc aggcggcgcg
     3241 cgccgccgcc agcgattcga ccgtgctgct gctcggcgag accggcaccg gcaaggaact
     3301 gatcgcgcag gcgatccatg ccgcctccgc gcgcgccgcg cggccgttcg tcggcatcaa
     3361 cgtcgcggcc gtgcccgatg gcctgctcga agccgagttc ttcggcagcg cgcccggcgc
     3421 ctataccggc gccgaccgcc gcgggcggga cggcaagttc aagctcgccg atggcggcac
     3481 gctgttcctc gacgagatcg gcgacatgcc tgcgcacctg caggcgaagc tgctgcgcgt
     3541 gctgcaggag caggagttcg agccgctcgg ctcgaaccgc gtcgagcgca tcgacgtgcg
     3601 cgtgatcgcc gccaccagcc gtgatctgcc ggcgcaggtc gccggggggc gctttcgcgc
     3661 cgatctgtgg ttccggctga acgtgctgcc gatccggctg ccgccgctgc gcgagcgccg
     3721 tgacgatctg ccgctgctcg ccggggcgct gctcgaccgg ctctcggccc gcgccggcgg
     3781 cgtgcgccgc gtgctcggcg cctcggcgct cgcggagctg gcgggctacg actggcccgg
     3841 caacacgcgc gagctcggca acgtgctcga acgcgcggtg atgctgagcg accggcgctg
     3901 ctacgaggcc gcggatctgg ccggcctgct gcgtccgccc gcggctgcgg ccgcatcggc
     3961 gccggccgcc cccgccgtgc agcgcctcgc cgatgccgtc gccgaggccg agcgcacggc
     4021 gatccgttcg gcgctcgccg tcagcggcgg acgcaaggtc gatgcggcga agctgctcgg
     4081 catctcgcgc gcgacgctgt acgaaaagct cgcgctgctc ggcctgcagg gcacgggctg
     4141 aatccgcggc ggcggctgcg ccaaagcggg gcgacgcaag gaatttgcac gttaccgttc
     4201 caatcaccgt tccaatggcg tgcgtcttgc tccagcatac gcgccttccc gccggtggcc
     4261 gcgcatctgc ttgtaaatgc ggccctgacg ggggatcggg cgccacgatg gtgcgacgca
     4321 gcaccattgt ggtgacactt cgtcccgcgg tgaccggccc ccgctggtac accgcatcat
     4381 gaacagtcta cgggggatgc aatgggcaag gtggcaatcg ttacgggcgg catcggtggc
     4441 ctgggaaccg cgatgtgcaa ggcgctgtgc gcccagggcc gtaccgtcgt ggcggcctac
     4501 taccccgccg aggccgacaa ggctgcggaa tggcagaagg cccgcaagga agaaggtctg
     4561 gacatcgccg tctatccggt ggacgtgagc gacctcgaaa gctgcgcgca gctcgtcgcc
     4621 aaggtcgagg ccgacctcgg tccggtcgag atcctggtca acaacgccgg catcacgcgc
     4681 gattccgtgt tcaagcgcat gaccgacgcc cagtggctgg ccgtcatcaa cacgaacctg
     4741 aacagcctgt tcaacatgac caagccggtg ttcgagaaga tgacctcgcg cggctggggc
     4801 cggatcatca acatctcgtc ggtcaacggc cagaagggcc agttcggcca ggccaactac
     4861 tcggccgcca aggccggtgc gcacggcttc acgatggcgc tggcgcagga aggcgccaag
     4921 gccggcgtga ccgtcaacac catctcgccg ggctacatcg gcaccgacat ggtcatggcc
     4981 atcgccgagg aagtccgcaa caagatcatc gcgacgatcc cggtcggccg tctcggcacg
     5041 ccggaagaca tcgcgcgcac ggtcgccttc ctggccgatg acgaagccgg cttcatcacc
     5101 ggcgccgaca tgtacgtcaa cggcggtctg tacatccact gatcgtccga ccggccggcg
     5161 ccgcagcgcc gcaagcgtca gaccccgaca ccgtccggat tcccggacgg tgtctttttt
     5221 tccggacggc gccggcacgc ccgcggaaat tcaaaaaaat tcagccgcgc ccgcacaaat
     5281 ccacgccgat acggccgcgg tcgtatcgga cgtgtccgga aatccggacg gagggctggc
     5341 ggcaagcatg caggtctgtt ctttaattca ttaaaaaaca ataagttgta ttttaattgc
     5401 cggctggcac ggtgcttggc tcatggaagt cagcccccgg gtgcaaaggg ggcattcgtc
     5461 caccaccgaa ggagcccacc gtgctcggca tacttggcat cgtgctgtcc ctggccttgc
     5521 tgatctttct cgcgtaccgc ggctggaacg tcatcttcat cgcgccgttc tgtgcgaccg
     5581 tggcgctgct gttcaactgg ggcgacgcgc cgctgctcgc gacctacacc cagaccttca
     5641 tgccggcgct cggcaactac ttcgccaagt tcttcccgct gttcctgctc ggcgcggtgt
     5701 tcggcaagct gat
//
LOCUS       DAOQJH010000062         5700 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_8132_length_5700_cov_4.310895, whole genome shotgun sequence.
ACCESSION   DAOQJH010000062 DAOQJH010000000
VERSION     DAOQJH010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 5700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5700
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8132_length_5700_cov_4.310895"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     regulatory      complement(33..127)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            218..733
                     /locus_tag="PLQ18_02470"
     CDS             218..733
                     /locus_tag="PLQ18_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017642635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HNE00543.1"
                     /translation="MQQQIRDAGTADLDGILAIYNDAVQHTTAIWNDTPVDRTNRAAW
                     LAARTAAGYPVLVAAAADGAVLGYASFGDWRAFDGYRHTVEHSVYVHRDARGRGTGHA
                     LLAALTGRARALGKHVMVAAIDADNLASLALHRRLGFTETGRMPQVGCKFGRWLDLAW
                     MQLRLDERQQP"
     gene            complement(740..1444)
                     /locus_tag="PLQ18_02475"
     CDS             complement(740..1444)
                     /locus_tag="PLQ18_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009046126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HNE00544.1"
                     /translation="MNSHRGISQQAAEAIRTLILEGGYAEGALLPSQRDLAERLGVSR
                     TSLREALSGLASRGEVRVQAGKGVFVGKGQDARGAVPAAAVDWAFGAQASPGEIFQMR
                     YGLEGYAAGLAVARTTPAVLDALAANVEALRVELRAGDFEAANRLDFEFHRRLIELSG
                     NRVLLDLLGRSAEPFIESQKLPFVRRERVMETHAEHVRILRALERQSASAATRTMQEH
                     IRRAAARIGVQFATPA"
     gene            1751..2530
                     /locus_tag="PLQ18_02480"
     CDS             1751..2530
                     /locus_tag="PLQ18_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019845236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HNE00545.1"
                     /translation="MKLLKTLLAAVCLSLTAATAHADALTDIQGAGVLKVAVPQDFPP
                     FGSVGLDLQPQGYDIDVARLLAKGLGVKVELVPVTSANRIPYLQTKKVDLVISSLGKN
                     PEREKTIDFSIAYAPFFLAVFGPQDAKITSADALAGKTVGVTRGSVEDLELSKIAPPT
                     ADLKRFEDNNTTISAYLSGQVGLIATGNVVIAAIAAKNPPRLPVEQFKLKNSPCFIGL
                     NKNEPALQAKVNELIEAAKKDGSLDAISKKWLKMPLPDLGA"
     gene            2544..3206
                     /locus_tag="PLQ18_02485"
     CDS             2544..3206
                     /locus_tag="PLQ18_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018004372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HNE00546.1"
                     /translation="MSYQFQFQELLPYTDQFVRGVWLTVQLTAVSTVLGLALGTAGAA
                     ARRYGNRLLRTLVGAYVEIVRNTPFIVQLFFIFFGLPNLGVRLTAGEAAVIAMVVNLG
                     AYSTEIIRAGLQAIPKGQVEAGQSLGMSGVQIFRYVIIRPALGKIWPALSSQCVIVML
                     GSSVVSQISAEELTFAANFIQSRNFRSFEVYAVTTLLYLALALGLRQVFGLIGRQLFA
                     RR"
     gene            3219..3875
                     /locus_tag="PLQ18_02490"
     CDS             3219..3875
                     /locus_tag="PLQ18_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003572120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HNE00547.1"
                     /translation="MPEFTLWDIARNLLLAARWTVLLSLIAFAGGAVTGLLLTLARVS
                     KYPLLRLLARGYIELFQGTPLLMQLFLAFFGLSLAGIEVSPWVAASLALTLWTSAFLA
                     EIWRGCVEAIPKGQGEASASLGLSWLEAMRYVILPQALRIAIPPTVGFSVQVIKGTAL
                     ASIIGFIELTKAGTMLNNATFQPFTVFSLVALMYFALCYPLSLYAHSLEAKLHAARRT
                     "
     gene            3856..4590
                     /locus_tag="PLQ18_02495"
     CDS             3856..4590
                     /locus_tag="PLQ18_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009051420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HNE00548.1"
                     /translation="MPLVELDHIEKWYGQNHVLKGVTLNVERGAVVSLIGRSGSGKST
                     LLRCINGLETFDAGRITVDGEVLDPKKTDLRRFRQHVGMVFQQFNLFPHLTVGRNIML
                     APTVVKKLPAKEAEAIAREMLGRVGLSDKFDAYPEQLSGGQQQRVAIARSLAMQPSVL
                     LCDEITSALDPELVGEVLKVLERLAADGMTLILVTHEMRFARDVGSHLVFMHQGRVWE
                     EGDPKTLMANPHTPELAQFIGAVAAA"
     gene            complement(4925..5293)
                     /locus_tag="PLQ18_02500"
     CDS             complement(4925..5293)
                     /locus_tag="PLQ18_02500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE00549.1"
                     /translation="MSLISEEYVAAYARATGTDHNHAREKLRRIKEPLRSRIVRAAMT
                     QASLGSQELHDPIEDEPLLRQVLEQAEQEAKMSLADQGIEMHMGYCHLFWEKKAEILA
                     NRYGITWFSPADMNPYVLYD"
     gene            complement(5472..>5700)
                     /locus_tag="PLQ18_02505"
     CDS             complement(5472..>5700)
                     /locus_tag="PLQ18_02505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE00550.1"
                     /translation="SRWRSGGRRSGLVRFGERTQELASASSHVAAPAPMNCASSGVCG
                     FAISVFEAPSTRLVGWVSFVNPAYELICPAS"
BASE COUNT          958 a         1899 c         1813 g         1030 t
ORIGIN      
        1 caggtgcaga ggacgaaacg gggggcggac ggcgaccggg cgccgaagct ccctccgccg
       61 gtgctaaccg gttcaggttc caagggtatt tctcagctcc gaatccggag cacccccgct
      121 tcgatccggc cgcgattaca gcacagtgtc acgcggattg cgcgcgccgt ccttccggtg
      181 tgccgccgca cggacccatt cgggagaaga accgcacatg cagcagcaga ttcgcgatgc
      241 cggcacggcc gacctcgacg gcatcctcgc catctacaac gacgccgtgc agcacacgac
      301 ggcgatctgg aacgacacgc ccgtcgatcg caccaaccgc gccgcctggc tcgcggcgcg
      361 cacggcggcc ggctatccgg tgctggtcgc ggccgctgcc gacggcgccg tgctcggtta
      421 cgcatccttc ggcgactggc gcgccttcga cggctaccgg cacacggtcg agcattcggt
      481 ctacgtgcac cgcgacgccc gcggccgcgg caccgggcac gccctgctcg cagcgctgac
      541 cggacgcgcc cgggcgctcg gcaagcacgt gatggtcgcc gccatcgacg ccgacaacct
      601 cgcctcgctg gcgctgcacc ggcggctcgg cttcaccgag accggccgca tgccgcaggt
      661 cggctgcaag ttcgggcgct ggctggatct ggcgtggatg cagttgcggc tggatgagcg
      721 gcagcagccc tgaatccgct cacgccggcg tcgcgaactg cacgccgatg cgtgcggcgg
      781 cgcggcggat gtgttcctgc atcgtgcgcg ttgccgcgct cgccgactgg cgctccagcg
      841 cgcgcaggat gcgcacatgc tcggcatggg tttccatgac ccgctcgcga cgcacgaacg
      901 gcagcttctg gctttcgatg aacggctcgg ccgagcgtcc gagcaggtcg agcagcacac
      961 ggttgccgct cagctcgatc agccggcggt ggaactcgaa gtcgaggcgg ttggcggcct
     1021 cgaagtcgcc ggcgcgcagt tcgacgcgca gcgcctcgac gttcgccgcc agcgcatcga
     1081 gcacggccgg cgtcgtgcgc gcgacggcga gcccggcggc gtagccctcc agcccgtagc
     1141 gcatctggaa gatttccccg gggcttgcct gcgccccgaa ggcccagtcc acggcggccg
     1201 ccggcaccgc gccgcgcgca tcctgcccct tgccgacgaa cacgcccttg ccggcctgca
     1261 cgcgcacctc gccgcgcgag gccagccccg agagcgcctc gcgcagcgag gtgcggctga
     1321 cgccgaggcg ctcggccagg tcgcgctgtg aaggcagcag cgcaccttcg gcgtagccgc
     1381 cttcgaggat cagcgtgcgg atcgcttcgg cagcctgctg ggaaatgccg cgatgactgt
     1441 tcatacctgt gtgatcgttc cgataccgga ttcctgagcc ggaccgagcg tactgccgac
     1501 gtggaaaccg tgcaaatcct gaattcatgg cgattttccg cttgcaccat tgcacggttt
     1561 tcgggcacac atctggtgca gaaatttcgg ttttttgact ctgcagcacc gctcaactgt
     1621 tcagactggt atgaacagtt gaggcgccat gcggctttgc cgccgatgca ctcatgctgg
     1681 cacggcggtt gcgatacgca gatgccgaac ccgtttcgcc ccttcccgtc cacctgccgg
     1741 agtccgtccg atgaagctgc tgaaaaccct gctcgccgcc gtctgcctgt cgctgaccgc
     1801 cgccaccgcg catgccgatg ccctcaccga catccagggc gccggcgtgc tgaaggtcgc
     1861 cgtgccgcag gacttcccgc ccttcggctc ggtcggcctc gacctgcagc cgcagggcta
     1921 cgacatcgat gtcgcgcggc tgctggcgaa gggcctcggc gtcaaggtcg agctggtgcc
     1981 ggtcaccagc gccaaccgca tcccgtacct gcagacgaag aaggtcgatc tggtcatctc
     2041 cagcctcggc aagaacccgg agcgcgagaa gaccatcgac ttctcgatcg cctacgcgcc
     2101 gttcttcctc gccgtgttcg gcccgcagga cgcgaagatc acctcggccg acgccctcgc
     2161 cggcaagacg gtcggcgtca cccgcggctc ggtcgaggac ctggaactga gcaagatcgc
     2221 gccgccgacg gccgacctga agcgcttcga ggacaacaac acgacgatct cggcctacct
     2281 gtccggtcag gtcgggctga tcgcgaccgg caacgtcgtc atcgccgcga tcgccgcgaa
     2341 gaacccgccg cgcctgccgg tcgagcagtt caagctcaag aactcgccgt gcttcatcgg
     2401 cctgaacaag aacgagccgg cgctgcaggc gaaggtcaac gagctgatcg aggccgcgaa
     2461 gaaggacggc tcgctcgatg caatctcgaa gaagtggctg aagatgccgc tgccggatct
     2521 cggtgcctga ggcggacgcc gcgatgagct accagttcca gttccaggag ctgctgccct
     2581 acaccgacca gttcgtgcgc ggggtctggc tgaccgtgca gctgacggcg gtctcgacgg
     2641 tgctcggcct cgcgctcggc accgccggcg ccgcggcccg gcgctacggc aaccggctgc
     2701 tgcgcacact cgtcggcgcc tacgtcgaga tcgtgcgcaa cacgccgttc atcgtgcagc
     2761 tgttcttcat cttcttcggg ctgccgaacc tcggcgtgcg cctgacggcc ggtgaagcgg
     2821 cggtgatcgc gatggtcgtg aatctcgggg cgtactcgac cgagatcatc cgcgccggcc
     2881 tgcaggcgat cccgaagggc caggtggagg ccggacagag cctcggcatg agcggcgtgc
     2941 agatcttccg ctacgtcatc atccgcccgg cgctcgggaa gatctggccg gcgctgtcga
     3001 gccagtgcgt gatcgtgatg ctcggctcgt cggtggtgtc gcagatctcg gccgaggaac
     3061 tgaccttcgc ggccaacttc atccagtcgc gcaatttccg cagcttcgag gtgtacgcgg
     3121 tgaccacgct gctgtacctc gcgctcgcgc tcggcctgcg tcaggtgttc ggcctgatcg
     3181 gccggcaact gttcgcgcgt cgctgacgga ggcccgccat gccggaattc acgctgtggg
     3241 acatcgcccg caacctgctg ctcgcggccc gctggaccgt gctgctgtcg ctgatcgcct
     3301 ttgccggtgg cgccgtcacc ggcctgctgc tgacgctggc gcgggtatcg aaatacccgc
     3361 tgctgcggct gctggcgcgc ggctacatcg agcttttcca gggcacgccg ctgctgatgc
     3421 agctctttct cgccttcttc ggcctgtcgc tcgccggcat cgaggtatcg ccgtgggtcg
     3481 ccgcgtcgct ggcgctgacg ctctggacca gcgccttcct cgccgagatc tggcgcggct
     3541 gcgtcgaggc gatcccgaag ggccagggcg aggcctcggc cagtctcggc ctgtcgtggc
     3601 tggaggcgat gcgctacgtg atcctgccgc aggcgctgcg catcgcgatc ccgccgaccg
     3661 tcggcttctc ggtgcaggtg atcaagggca cggcgctcgc atcgatcatc ggcttcatcg
     3721 agctgaccaa ggccggcacg atgctgaaca acgccacctt ccagcccttc accgtgttct
     3781 cgctcgtggc gctgatgtac ttcgcgctgt gctacccgct gtcgctgtac gcgcactctc
     3841 tggaggcaaa gctccatgcc gctcgtagaa cttgaccaca tcgaaaaatg gtacggccag
     3901 aaccacgtgc tgaagggcgt gacgctgaac gtcgagcgcg gcgcggtcgt gtcgctgatc
     3961 gggcgcagcg gttcgggcaa gagcacgctg ctccggtgca tcaacgggct ggagaccttc
     4021 gatgccgggc gcatcaccgt cgatggtgaa gtgctggacc cgaagaagac cgacctgcgc
     4081 cgcttccgcc agcacgtcgg catggtgttc cagcagttca acctgttccc gcacctgacg
     4141 gtcgggcgca acatcatgct ggcgccgacg gtcgtgaaga agctgccggc gaaggaagcc
     4201 gaagccatcg cccgcgagat gctcggccgc gtcggcctgt cggacaagtt cgacgcctac
     4261 cccgagcagc tctccggcgg ccagcagcag cgcgtcgcga tcgcccgctc gctggcgatg
     4321 cagccctcgg tgctgctctg cgacgagatc acctcggcgc tcgacccgga actggtcggc
     4381 gaggtgctga aggtgctcga acgcctggcc gccgacggca tgacgctgat cctggtcacg
     4441 cacgagatgc gctttgcacg cgatgtcggc agccatctgg tgttcatgca tcagggccgc
     4501 gtctgggagg aaggcgaccc gaagacgctg atggcgaatc cgcatacacc ggaactggcg
     4561 cagttcatcg gggcggtggc ggcggcgtaa agggttgatc ctgccaatct tcgagtcctt
     4621 ttcccggcgc gggagaggac ttcgggagag tgtgagcatc agcgtattga tcgtgtgaaa
     4681 cacctaaaac cgtcattccc cctgaaacgg gaatgacggc aatggaagca ttggtaatcg
     4741 caagcatgtg aagtgtgttc tgttcggcac gccagtctgt gtgtctcggt atacattttc
     4801 aggcgtcagc ttatttgcat gccgcgaaag gcgaacaaca gcgcgcagca agcatgatgc
     4861 gctttgatac gccacacact gcgcgcctga tctgccctac aaactaaaat ttggagtcat
     4921 tacctcaatc atacagaaca tacggattca tatctgcagg cgagaaccat gtgatcccgt
     4981 accgattggc cagaatctct gccttctttt cccagaaaag gtggcaatag cccatatgca
     5041 tttcaatacc ttggtcagca aggctcatct ttgcctcctg ctccgcctgt tccagaactt
     5101 gtcgcagcag cggttcgtcc tcgatagggt catggagttc ttgcgaccca agcgatgcct
     5161 gcgtcatggc agcgcgaact atacgactgc gaagcggctc ctttattcga cgcagttttt
     5221 ctcgtgcatg attgtgatcg gttcccgttg ctcgagcgta cgctgcgacg tactcttcgc
     5281 tgatcaatga cataccaaga ctccttaagt gaagtagact gcgtttccgt tgtcgaattg
     5341 cttctttgac aaaaatggat ggtgataaaa ccgtttgtcc tggcggattt ggcgcttggg
     5401 ttgtctaaac cgtcaccgta gatgcgtagt tacaagccgc aagacacaag gcttggttat
     5461 gcgggcatca ctcagctcgc tgggcagatc agctcgtagg ctgggttgac gaaggaaacc
     5521 cagcctacga gccgggtgga aggcgcctcg aagacgctga tggcgaatcc gcacacgccg
     5581 gaactggcgc agttcatcgg ggcaggggcg gcgacgtgag agcttgcgct tgccaattcc
     5641 tgagtccttt caccgaaccg caccaggccg gaccgccgcc cgccgctacg ccaccttgat
//
LOCUS       DAOQJH010000063         5680 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_8174_length_5680_cov_3.937956, whole genome shotgun sequence.
ACCESSION   DAOQJH010000063 DAOQJH010000000
VERSION     DAOQJH010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 5680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5680
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8174_length_5680_cov_3.937956"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1734)
                     /locus_tag="PLQ18_02510"
     CDS             complement(<1..1734)
                     /locus_tag="PLQ18_02510"
                     /inference="COORDINATES: protein motif:HMM:NF013974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNE00551.1"
                     /translation="MSALLDGCTAIARGAWEGGASFAAGYPGSPVTGVLDATAGYPEI
                     RRQWAANEKIALETALGAAWSGARALVVMKHVGLNVAADPLYNAPYTGVRGALVIVVG
                     DDPGASSSQNEQDTRLVAKAAGVPVLEPSSVAEALVFTRLAFEISARHDLPVIVRVTT
                     PIAYGSTGAVAGARWPVPAAAGHARPIQKYLLLPAFVPARHTALIERLSALGASPAAR
                     WFCETSLPPAAAAGTRHPVGIVCAGFPAAAVRELCADTVPVLTLGLVHPLPEAPIRAF
                     AQHCERLLVVEESSHYLEDAIRALGLPVARRSHYDGVGSFGPKQLLSDEAPVVNALVQ
                     PLIDAAEQRRRHIPILAPRTPGFCAGCSHRGIFSLLAKRDLYVVGDIGCNTLGAIQPY
                     GALHANLCMGASISVLQGYLAALGPDAAQRTIAVLGDSTFFHSGLPAILSAVHNGLPG
                     TVLVLDNAGSAMTGHQQTAHARDAEGWRTLLGALGVPDVRVVDALDLGELDAALDAAL
                     ATDALSVVVLKGDCVQSLPRKGPTNYRYTVRTDACSGCGDCLKVDCPAIVQLAASAAG
                     GPRKVAITHECI"
     gene            complement(1731..2639)
                     /locus_tag="PLQ18_02515"
     CDS             complement(1731..2639)
                     /locus_tag="PLQ18_02515"
                     /inference="COORDINATES: protein motif:HMM:NF012901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HNE00552.1"
                     /translation="MSVVLMFPGLGSEYPELLARQCAEDPQAAERLADWRERLGVPLT
                     DTPPAEPRARELARQMAIHAVNLLAWERHGAAYPQAAVCGHSLGFYAAAVAAGALDAD
                     ASFAWIGAVFERAWARFEHDRDCVYVLTARDTLDGPALAAAWGVELLAVNGPCQIVVY
                     GAPAVLEALCAALAGRLVRHGPLGSCCPFHSVRMAPVCVEVAHWLAASGLQPRAPQRP
                     LWSHLDATRLMDAEAVRATLITQPQLRVRWDALIGRLAADGHDTFVELGPNRILAQAA
                     RWIVPGSLTVLSTDSARRIRRAGSPA"
     gene            complement(2657..3421)
                     /locus_tag="PLQ18_02520"
     CDS             complement(2657..3421)
                     /locus_tag="PLQ18_02520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE00553.1"
                     /translation="MHAPAARTEALHAGRQRPHADLPAGPVLHQPSAHGPRSSLSVCV
                     AGLGGLGATTLNARLAELLRTSHGEVCAIDQLGVARRRASVTNLLRAGRGVRCAGWCG
                     SPYDVLIALEESESLRQLDRIGPQTLVLLADCRVETIAGYSGAPLPSHAVVRSTLDQH
                     AGYVLQLPVTRELGRHQLNGAHGTALFFGAFAALLGLTVAEAEHHVSTGHARGAATLL
                     ANTGHAAAVRAGLLGWLNGGHRQHAATAVVTPAVAA"
     gene            3949..4821
                     /locus_tag="PLQ18_02525"
     CDS             3949..4821
                     /locus_tag="PLQ18_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNE00554.1"
                     /translation="MSADHRRRSGLLLVLAAAAGFGLMAVFARSAYAAGVNPPTLLAL
                     RFTLAALLLVAIVQARARPWPRGRALIGLIAMGGGFYTLIAQCYFVALTQASAALVAL
                     VLYTFPVLVMAINVACFGERLTVAKCGAALLAMAGLALTIGGELHGSAGGIALALVAA
                     AGYAAYILIGDRVARGVDPLCGGAVVIAASAVTSALLALAEGVTLPQTGGGWLAVTGI
                     ALVSTVFAMTAFLEGLSRVGSTTAAMASTFEPVVTVASAAWLLGETVSPARLAGGGLI
                     LAAVVLLARAPHRP"
     gene            4863..>5680
                     /locus_tag="PLQ18_02530"
     CDS             4863..>5680
                     /locus_tag="PLQ18_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008165827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HNE00555.1"
                     /translation="MTMRPRRSVLYMPGSNARALDKARSLDVDALILDLEDSVAPEAK
                     GLGREQIAAALTEGGYGHREVVVRVNALSTPWGHDDVRALAKLGADAFLFPKIDSPAQ
                     VIEAVTALDAAGAPSGTGVWIMAETPLGILNIAAIAACSPRLRCIVLGTSDLAKDLRA
                     RHSPGRIAFVTSLGLCVLAARASGLDALDGVHLDLDDEAGLAAVCEQGRDMGFDGKTL
                     IHPRQIAAANRAFAPSAAEIGEARAIIHGWTQAAAEGKGVCRVNAYQIGRESVGS"
BASE COUNT          776 a         2017 c         2108 g          779 t
ORIGIN      
        1 gatgcattca tgggtgatgg cgaccttgcg cgggccgccg gcggccgagg ccgcgagctg
       61 cacgatcgcc gggcagtcga ccttcaggca gtcgccgcag ccgctgcagg cgtcggtgcg
      121 caccgtgtag cggtagttgg tcgggccctt gcgcggcagg ctctgcacgc agtcgccctt
      181 gagcacgacc accgacagcg catcggtggc gagcgcggca tcgagtgcgg catcgagttc
      241 gccgagatcg agcgcatcga cgacgcgcac gtccggcacg ccgagcgcgc cgagcagcgt
      301 gcgccagccc tcggcgtcgc gggcatgggc ggtctgctgg tggccggtca tcgccgagcc
      361 ggcgttgtcg agcaccagca ccgtgcccgg caggccgttg tgcaccgcgc tcaggatcgc
      421 cggcaggccg gagtggaaga aggtcgagtc gccgagcacc gcgatcgtgc gctgcgcggc
      481 gtcggggccg agcgcggcca gatagccctg cagcacgctg atgctcgcgc ccatgcagag
      541 attcgcgtgc agcgcgccgt agggctggat cgcgccgagc gtgttgcagc cgatgtcgcc
      601 gaccacgtac aggtcgcgct tcgcgagcag cgagaagatg ccgcggtgcg agcagccggc
      661 gcagaagccg ggcgtgcgcg gcgcgaggat cgggatgtgc cggcggcgct gctcggcggc
      721 atcgatcagc ggctgcacca gcgcgttgac gaccggcgcc tcgtcgctga gcagctgctt
      781 cggaccgaag ctgccgacgc cgtcgtaatg actgcggcgg gcgaccggca ggccgagcgc
      841 gcggatcgca tcctccaggt agtgcgagct ttcctcgacc accagcaggc gctcgcagtg
      901 ctgcgcgaag gcgcggatcg gtgcttccgg cagcggatgc acgaggccga gcgtcagcac
      961 cggcacggtg tcggcacaga gttcccgcac cgccgcggcc gggaagccgg cgcagacgat
     1021 gccgaccggg tggcgcgtgc cggccgcagc cgccggcggc agcgaggtct cgcagaacca
     1081 gcgcgcggcg ggactggcgc cgagcgccga cagccgctcg atcagcgccg tgtgccgcgc
     1141 cggtacgaag gccggcagca gcaggtactt ctggatcggc cgcgcatggc cggcggcggc
     1201 cggcaccggc cagcgcgccc cggcgaccgc gccggttgag ccgtaggcga tcggcgtggt
     1261 cacgcgcacg atcaccggca ggtcgtggcg ggcgctgatc tcgaaggcga ggcgcgtgaa
     1321 caccagcgcc tcggccaccg acgagggctc cagcaccggc acgccggcgg ccttggcgac
     1381 caggcgcgta tcctgctcgt tctgcgagga cgaggcgccc ggatcgtcgc cgacgacgat
     1441 gaccagcgca ccgcgcacgc cggtgtacgg cgcgttgtag agcgggtcgg cggcgacgtt
     1501 gaggccgacg tgcttcatga ccaccagcgc acgcgcaccg gaccaggcgg caccgagggc
     1561 ggtttccagc gcgatcttct cgttcgccgc ccactggcgg cggatctccg ggtagccggc
     1621 cgtggcatcg agcacgccgg tcaccggcga acccggatag ccggccgcga agctggcgcc
     1681 gccttcccag gcgccgcggg cgatggccgt gcagccgtcg agcagggcac tcatgcggga
     1741 ctcccggcgc gccggatgcg gcgcgcgctg tcggtggaaa gcacggtgag cgaacccggc
     1801 acgatccagc gcgcggcctg cgcgaggatg cggttcgggc cgagttcgac gaaggtgtcg
     1861 tgaccgtcgg cggcgagccg cccgatcagc gcgtcccagc gcacgcgcag ctgcggctgc
     1921 gtgatcagcg tcgcgcgcac ggcctcggca tccatgagcc gcgtggcgtc gaggtgcgac
     1981 cagagcgggc gctgcggcgc acgcggctgc aggccgctcg cggcgagcca gtgcgcgacc
     2041 tcgacacaca ccggcgccat gcgcaccgag tggaaggggc agcacgaacc gagcgggccg
     2101 tggcgcacga ggcgcccggc gagggccgcg cacagggctt cgagcaccgc cggcgcgccg
     2161 tagacgacga tctggcaggg gccgttgacg gcgagcagct ccactcccca ggccgcggcg
     2221 agcgccggac cgtcgagcgt gtcgcgggcg gtcagcacgt acacgcagtc gcggtcatgc
     2281 tcgaagcgcg cccaggcccg ctcgaacacc gcgccgatcc acgcgaacga ggcgtcggca
     2341 tcgagcgcac cggcggcgac cgccgcggcg tagaagccga ggctgtggcc gcagaccgcg
     2401 gcctgcggat acgccgcgcc gtggcgctcc caggcgagca ggttcacggc gtggatcgcc
     2461 atctgccggg ccagctcgcg tgcgcgcggc tcggccggcg gcgtgtcggt cagcggcacg
     2521 ccgaggcgct cgcgccagtc ggcgaggcgt tcggcggcct gcgggtcctc ggcgcactgg
     2581 cgcgcgagca gttccggata ttcggaaccg aggccgggaa acatcagcac gacggacatc
     2641 gcgggcgctc cggggctcag gccgcaacgg ccggcgtgac gacggccgtc gccgcgtgct
     2701 ggcggtgacc gccgttcagc cagccgagca ggccggcgcg cacggcggcc gcgtggccgg
     2761 tgttggcgag cagcgttgcg gcaccgcggg cgtggccggt cgagacatgg tgttcggctt
     2821 cggcgaccgt gagcccgagc agcgccgcga aggctccgaa gaacagcgcc gtgccgtgcg
     2881 cgccgttgag ctggtggcgg ccgagttcgc gcgtcaccgg cagctgcagc acgtagccgg
     2941 cgtgctggtc gagggtgctg cgcaccacgg cgtggctcgg cagcggcgcg ccgctgtaac
     3001 cggcgatggt ctcgacgcgg cagtcggcga gcagcaccag cgtctgcggg ccgatgcgat
     3061 cgagctggcg caggctctcg gattcctcca gcgcgatcag cacgtcgtac ggcgaaccgc
     3121 accagccggc gcagcgcacg ccgcgcccgg cgcgcagcag gttggtcacc gaggcgcggc
     3181 ggcgggcgac gccgagctgg tcgatcgcgc agacctcgcc gtggctcgtg cgcagcagtt
     3241 ccgcgagccg cgcgttcagc gtcgtcgcgc cgaggccgcc gaggccggca acgcatacgg
     3301 acagggagct gcgcgggcca tgcgcggacg gctggtgcag gaccggtccg gcgggcaggt
     3361 cggcgtgggg acgctgacgg ccggcgtgca gggcttcggt gcgggcggcg ggtgcgtgca
     3421 tgcgatcaac ctgcgtctgg aggagtgaga agataaattt ccggtaattc cggaaaaggc
     3481 attccggaac aacttgatgg aacggaaatc tacggacctg ctggttatcc gtcattacga
     3541 cgggcatcgt attttggaga cgcaagcagt gcttaccacc gcaaattttt agctttttcc
     3601 ccgtacggcg gatgctgtac tcaaatcaat ggattcaagc gcttgcgttt cttgctgtcc
     3661 gattgcccgg ctgtgagtcc ggttttcatt caaacttcgt catctgcgcg tcgggttgca
     3721 tgactgccga aagtccgccc cacgtggggc gacgggcgct gcatcgatgc gccgccggcg
     3781 gtcccggggc cggttccggc ctccgcgacg ccgcgccggt catgcaggcc atgcagatat
     3841 ccgatgagac ggacaggcgc cgtgatctct gcagatgcac gctgcgtctg cagccggcag
     3901 aatccgcggc cccgggcgcc cggcgcccgc agccggtcct gcccgtccat gtctgccgat
     3961 caccgccgcc gcagcggcct tctgctcgtg cttgccgctg ctgccggctt cggactgatg
     4021 gcggtgttcg cgcgcagcgc ctacgccgcc ggcgtgaatc cgccgacgct gctggcgctg
     4081 cgcttcacgc tggcggcgct gctgctggtc gcgatcgtgc aggcgcgcgc ccggccgtgg
     4141 ccgcgcggcc gcgcgctcat cgggctgatc gcgatgggcg ggggcttcta cacgctgatc
     4201 gcgcagtgct acttcgtggc gctgacgcag gcgagtgccg cgctggtggc gctggtgctg
     4261 tacacgtttc cggtgctggt catggcgatc aacgtcgcct gcttcggcga gcgcctgacg
     4321 gtggcgaagt gcggcgcggc gctgctcgcg atggcggggc tcgcgctgac catcggcggc
     4381 gagctgcacg gcagcgccgg cggcatcgcg ctggcgctgg tggccgcagc cggctacgcc
     4441 gcctacatcc tgatcggtga ccgcgtcgcg cgcggcgtcg atccgctctg cggcggcgcg
     4501 gtcgtgatcg cggccagcgc cgtcaccagc gcactgctgg cgctggccga gggcgtgact
     4561 ctgccgcaga ctggcggcgg ctggctggcc gtgaccggca tcgcgctggt ctcgaccgtg
     4621 ttcgcgatga ccgcgtttct cgaagggctg tcgcgcgtcg gctcgaccac tgcggcgatg
     4681 gcctcgacct tcgagcccgt ggtcacggtc gccagcgccg cgtggctgct cggggaaacc
     4741 gtcagccccg cccggctcgc cggcggcggg ctgatcctcg ccgccgtcgt gctgctcgcg
     4801 cgtgccccgc atcgcccatg acgggcagca cgccggctca catccatata aaggagtcac
     4861 gcatgacgat gcgcccccga cgatccgtgc tctacatgcc cggttccaat gcccgcgccc
     4921 tcgacaaggc gcgcagcctc gatgtcgacg ccctgatcct cgatctggag gattcggtcg
     4981 cccccgaggc caagggactc ggccgcgagc agatcgccgc cgccctgacc gaggggggct
     5041 acgggcaccg cgaggtcgtc gtgcgcgtca acgcgctgtc cacgccctgg ggccacgatg
     5101 acgtgcgcgc cctcgcgaag ctcggcgctg acgccttcct gttcccgaag atcgattcgc
     5161 cggcgcaggt gatcgaggcc gtcaccgcgc tcgatgccgc cggcgccccg tccgggaccg
     5221 gcgtctggat catggccgag acgccgctcg gcatcctcaa catcgccgcg atcgccgcct
     5281 gctcgccgcg gctgcggtgc atcgtcctcg gcacctcgga cctcgccaag gacctgcgcg
     5341 cccgccacag ccccgggcgc atcgccttcg tgacctcgct cggcctctgc gtgctcgccg
     5401 cccgcgccag cggcctcgac gcgctcgacg gcgtgcacct cgacctcgac gacgaggccg
     5461 gcctcgccgc agtctgcgag cagggccgcg acatgggctt cgacggcaag acgctgatcc
     5521 accccaggca gatcgccgcc gccaaccgcg ccttcgcgcc gtccgcggcc gaaatcggcg
     5581 aagcccgcgc catcatccac ggctggaccc aggccgctgc cgagggcaag ggcgtctgcc
     5641 gcgtcaacgc ctaccagatc ggccgcgagt cggtcggctc
//
LOCUS       DAOQJH010000064         5679 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_8177_length_5679_cov_4.701991, whole genome shotgun sequence.
ACCESSION   DAOQJH010000064 DAOQJH010000000
VERSION     DAOQJH010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 5679)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5679)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5679
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8177_length_5679_cov_4.701991"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..430
                     /gene="mtnB"
                     /locus_tag="PLQ18_02535"
     CDS             <1..430
                     /gene="mtnB"
                     /locus_tag="PLQ18_02535"
                     /EC_number="4.2.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002613658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methylthioribulose 1-phosphate dehydratase"
                     /protein_id="HNE00556.1"
                     /translation="HGRPARAGQRSSAETGLHLQLYRRDPAIGAVLHTHSVNATVLSM
                     LAGGALVLRDLEVLKAFPGIATHESAVCVPVLANDQDIARLAARVAEVLDRDPGCCGY
                     LIAGHGFYTWGATVADAVRHVEAFEFLFECESLMRRMGRP"
     gene            427..972
                     /locus_tag="PLQ18_02540"
     CDS             427..972
                     /locus_tag="PLQ18_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020393908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin"
                     /protein_id="HNE00557.1"
                     /translation="MSKLRVYRDDAPDTPIVEHTLGADIALELEGPEVLFSRWEADRE
                     LPADASNDDIIGAYRAQIDVLMTRYGFHAVDVVNVTPTHPDKDALRQKFLSEHTHSDY
                     EVRFFVEGSGQFYIHHADRVYMIFCQAGDLLSIPANMTHWFDMGPNPSFKCIRLFTTP
                     EGWVANFTGSDIAEHFPRLVN"
     gene            989..1684
                     /gene="mtnC"
                     /locus_tag="PLQ18_02545"
     CDS             989..1684
                     /gene="mtnC"
                     /locus_tag="PLQ18_02545"
                     /EC_number="3.1.3.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020158388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acireductone synthase"
                     /protein_id="HNE00558.1"
                     /translation="MSVAIRAILTDIEGTTSSIAFVHEVLFPYARARLPAWLAAHAGE
                     PEVAGQLAATRAEAGLPPEAPLAQVIEVLLGWIDADRKATPLKALQGRLWADGYAAGD
                     FHGHVYADVPPCLRAWQAAGLVLAVYSSGSVQAQQQLFAHTACGDLTPLFSAYFDTHI
                     GHKREPASYTAICAALGLAPAGVLFLSDITEELDAARSAGLATVQLVRDGTPDPQAAH
                     RQVTAFTAIDPYA"
     gene            1800..2591
                     /locus_tag="PLQ18_02550"
     CDS             1800..2591
                     /locus_tag="PLQ18_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011808145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNE00559.1"
                     /translation="MTPETPEPVPALEIVDLHKCFGSLEVLKGISLSARDGDVISVLG
                     SSGSGKSTCLRCINLLEQPTSGTIRVKGETLKLARDRGGQLASTDPKQLARIRSRLAM
                     VFQSFNLWAHMTVLDNLIEAPVHVLKLGRAEAIERAERLLHKVGLYERRNYYPAHLSG
                     GQQQRAAIARALAMEPDAILFDEPTSALDPELVGEVLKVMRVLAEEGRTMIVVTHEMG
                     FAREVSSRVVFLHQGRVEEDGPPAEVFGHPRSERCRQFLSGKLKG"
     gene            2667..3458
                     /locus_tag="PLQ18_02555"
     CDS             2667..3458
                     /locus_tag="PLQ18_02555"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008987709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNE00560.1"
                     /translation="MKKIALAALGCVLALGIGFGSVADAKEWKKVRIATEGAYPPFNF
                     VDADGKLAGFEIDLANALCAEMKVECTLVKQDWDGMIPALKARKYDLIMASMSITDER
                     KQQVDFSDWYYRMPARFVAKKGAGLTVTPEGLKGKTIGVQRETVSDKFLTDNYGSSVT
                     IKRYATQDEVYLDLTAGRLDAYVADIIVSRDAFLSKPEGKDYEFTGPEFSDPKWFGDG
                     IGAAMRKQDNDLTALFNKALKALIANGTYQKVAAKYPDFDSYLRK"
     gene            3795..4484
                     /locus_tag="PLQ18_02560"
     CDS             3795..4484
                     /locus_tag="PLQ18_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002686047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNE00561.1"
                     /translation="MLDFHGYGASVFEGAWLTLEVALMSLVVAVALGLAGALAKLSRS
                     LVLRTIAGLYTTIIRGMPDLVLMLLIYFGGQTLVNAISDALGWEYIDINPFVAGVLTI
                     GLIFGAYMTETFRGALMAVPKGQLEAGYAYGMSATQVFVRIHLPQMLRYALPGFGNNW
                     LVLLKTTALVSVIGLDDIVRKAQLAAGATRLPFTFYGAVLLGFLAFTTVSVLLLRWAE
                     RRYSIGYERME"
     gene            4491..5183
                     /locus_tag="PLQ18_02565"
     CDS             4491..5183
                     /locus_tag="PLQ18_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019933499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNE00562.1"
                     /translation="MEFGVILDNWPLYLEGLVTTVELTGLCLILGLLLAIPLGTVQAV
                     GPLWARGLVFAYSYVFRGTPLLVQTFLIYYGLGQFEAVQQSVFWPLLKEAYWCALIAF
                     TLNTGAYTTEIIRGSIVTMPRGEIEAARACGMDTLLMLRRIVLPSAFRRALPAYGNEV
                     IFMLQGSSIASTITLVDLTGAARVINSRYYLPYEAFLTAGLLYMALSFLLFAGFRKLE
                     QRWFAHLRPREH"
     gene            complement(5243..>5679)
                     /locus_tag="PLQ18_02570"
     CDS             complement(5243..>5679)
                     /locus_tag="PLQ18_02570"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HNE00563.1"
                     /translation="VVVLGDAADGFHYENLNHALQLLLGGAQLVAMADNRYFRDGERL
                     ALDIGPFRAALEYASGVSATIIGKPAQAMFETGCARLGLPPQRVAMIGDDAEADVAAA
                     LAAGLGAAVLVRTGKYRDGDERKCPRAGLADDFAAAVEMLLD"
BASE COUNT          880 a         2003 c         1877 g          919 t
ORIGIN      
        1 gcacggccgc ccggcccggg ccggtcagcg ttcctcggcc gaaaccggcc tgcacctgca
       61 gctgtaccgg cgcgatccgg ccatcggcgc tgtgctgcac acgcactcgg tcaacgcgac
      121 ggtgctgtcg atgctcgccg gcggggcact ggtgctgcgc gatctcgaag tgctgaaggc
      181 gttcccgggc atcgccacgc acgaaagcgc cgtgtgcgtg ccggtgctcg ccaacgatca
      241 ggacatcgcg cgcctcgccg cgcgcgtcgc cgaagtgctc gatcgtgatc cgggctgctg
      301 cggctatctg atcgccggcc acggcttcta cacctggggc gcgacggtcg ccgacgcggt
      361 acgccacgtc gaggccttcg agttcctgtt cgaatgcgaa tccctgatgc ggaggatggg
      421 cagaccatga gcaagctgcg tgtttaccgg gacgacgccc cggacacccc gatcgtcgaa
      481 cacacgctcg gcgccgacat cgcgctggag ctcgaaggcc cggaggtgct gttctcgcgc
      541 tgggaggccg accgtgaact gccggccgat gccagcaacg acgacatcat cggtgcctac
      601 cgcgcgcaga tcgacgtgct gatgacgcgc tacggcttcc acgccgtcga tgtcgtcaac
      661 gtcacgccga cgcacccgga caaggacgcg ctgcgccaga agttcctgag cgagcacacg
      721 cactcggact acgaggtgcg cttcttcgtc gagggcagcg gccagttcta catccaccac
      781 gccgatcgcg tgtacatgat cttctgccag gccggcgatc tgctgagcat cccggcgaac
      841 atgacgcact ggttcgacat ggggccgaac ccgagcttca agtgcatccg gctgttcacg
      901 acgcccgagg gctgggtcgc gaacttcacc ggcagcgaca tcgccgagca cttcccgcgg
      961 ctggtcaact gagccggcac ggcagcgcat gtccgtggcg atccgcgcga tcctgaccga
     1021 catcgaaggc acgaccagct cgatcgcctt cgtgcacgag gtgctgttcc cgtatgcgcg
     1081 cgcgcggctg cccgcctggc tggccgcgca cgccggcgag ccggaagtcg ccggacagct
     1141 cgccgcgacc cgtgccgagg ccggtctgcc gccggaggcg ccgcttgcgc aggtcatcga
     1201 agtgctgctc ggctggatcg acgccgatcg caaggccacg ccgctgaagg cactgcaggg
     1261 ccggctgtgg gccgacggct acgcggccgg cgatttccac ggccacgtct atgccgacgt
     1321 gccgccctgc ctgcgcgcct ggcaggcggc cggtctcgtg ctcgcggtgt attcgtccgg
     1381 ctcggtgcag gcgcagcagc agctgttcgc gcataccgcc tgcggtgacc tgacgccgct
     1441 tttttcggcg tacttcgaca cgcacatcgg ccacaagcgc gagccggcgt cctacacggc
     1501 gatctgcgcc gcgctcggcc tcgcgccggc cggggtgctg ttcctgtccg acatcaccga
     1561 agaactcgac gccgcgcgca gcgcagggct tgcgaccgtg cagctcgtgc gcgacggcac
     1621 acccgatccg caggccgcgc accggcaggt caccgccttc accgccatcg acccgtacgc
     1681 ctgagccgcc gccggcgctt gtcgtgcgcc ggcgaccggc tgctatgctc gggcgcaacg
     1741 cgatcgtcac cgatccgtcg tccgcacgtt cacgctcacg ccccgccaga cgagacctga
     1801 tgactcccga gactcccgaa ccggtcccgg cgctggaaat cgtcgacctg cacaaatgct
     1861 tcggctcgct cgaagtgctg aagggcatct cgctgagcgc ccgcgacggt gacgtgatct
     1921 cggtgctcgg gtcctccggc tccggcaaga gcacctgcct gcgctgcatc aacctgctcg
     1981 aacagccgac ctccggcacg atccgggtca agggcgagac gctgaagctc gcgcgcgacc
     2041 gcggcggcca gctcgccagc accgacccga agcagctcgc gcgcatccgc tcgcggctgg
     2101 cgatggtgtt ccagagcttc aacctgtggg cgcacatgac cgtgctcgac aacctgatcg
     2161 aggcgccggt gcacgtgctc aaactcgggc gcgccgaggc gatcgagcgc gccgagcgcc
     2221 tgctgcacaa ggtcggcctg tacgagcggc gcaactacta tccggcgcac ctctccggcg
     2281 gccagcagca gcgcgcggcg atcgcgcggg cgctggcgat ggagcccgat gccatcctgt
     2341 tcgacgaacc gacctcggcg ctcgatcccg agctggtcgg cgaggtgctg aaggtcatgc
     2401 gcgtgctcgc cgaggaaggc cgcacgatga tcgtcgtcac gcacgagatg ggcttcgcgc
     2461 gcgaggtctc cagccgcgtc gtgttcctgc accagggccg ggtcgaggaa gacggcccgc
     2521 ccgccgaggt gttcggccac ccccgttccg agcgctgccg gcagttcctg tccggcaagc
     2581 tcaagggctg acgccggtcc ggccgccaga gcgtccgccc gcccgtccgc atcgtcacgt
     2641 tcatccccgg aggcttgcgt accaacatga agaagatcgc cctcgctgcg cttggctgcg
     2701 tgctcgcact cggtatcggt ttcggcagcg tcgccgacgc gaaggaatgg aagaaggtgc
     2761 gcatcgccac cgagggcgcc tatcccccgt tcaacttcgt cgatgccgac ggcaagctcg
     2821 ccggcttcga gatcgacctc gccaacgcgc tgtgcgccga gatgaaggtc gaatgcacgc
     2881 tggtcaagca ggactgggac ggcatgatcc cggcgctgaa ggcgcgcaag tacgacctga
     2941 tcatggcctc gatgtcgatc accgacgagc gcaagcagca ggtcgatttc tccgactggt
     3001 actaccggat gccggcgcgc ttcgtcgcca agaagggcgc cggcctgacc gtcacgcccg
     3061 aagggctcaa gggcaagacc atcggcgtgc agcgcgagac ggtctccgac aagttcctga
     3121 ccgacaacta cggatcgagc gtgacgatca agcgctacgc gacccaggac gaggtctacc
     3181 tcgacctgac cgccggccgg ctcgatgcct acgtcgccga catcatcgtc agccgcgatg
     3241 ccttcctgtc caagcccgaa ggcaaggact acgagttcac cggccccgag ttcagcgatc
     3301 cgaagtggtt cggcgacggc atcggcgcgg cgatgcgcaa gcaggacaac gacctgacgg
     3361 cgctgttcaa caaggcgctg aaggcgctga tcgccaacgg cacctaccag aaggtcgcgg
     3421 cgaagtaccc ggacttcgat tcgtatctgc gcaagtgagg atgtgatcga gccgggtcca
     3481 cactgcgatc ggaacgaaac agcccctccc cccttgcggg ggaggggttg gggagagggg
     3541 tgaacgcccg ggcgcagtgc cggcgtctcc ccctctcccc cggcccctct cccgcgaggg
     3601 gagaggggag atcgaagccg gaccgcaggt ggccggttcc tccggtccac ggtatcgctt
     3661 ggctgacatg gatatcgatc ccgcacggag cggggctgaa aagcgaagcg ctccggtggg
     3721 aatggcatcg atcccggtgc cgatgtccgc tgcccatccc tgattcatcc gccattccac
     3781 ccggacgctt gccgatgctc gatttccacg gatacggcgc ctcggtgttc gaaggcgcat
     3841 ggctgacgct cgaagtggcg ctgatgtcgc tggtggtcgc cgtcgcgctc ggccttgccg
     3901 gtgcgctggc gaagctgtcg cgctcgctgg tgctgcgcac gatcgccggg ctgtatacga
     3961 ccatcatccg cggcatgccc gatctggtgc tgatgctgct gatctacttc ggcggccaga
     4021 cgctggtgaa cgccatcagc gatgcgctcg gctgggagta catcgacatc aatcccttcg
     4081 tcgccggcgt gctgaccatc ggcctgatct tcggcgccta catgaccgag accttccgcg
     4141 gcgcgctgat ggcggtgccg aagggccagc tcgaagccgg ctacgcctac ggcatgagcg
     4201 ccacgcaggt gttcgtgcgc atccacctgc cgcagatgct gcgctacgcg ctgccgggct
     4261 tcggcaacaa ctggctggtg ctgctgaaga ccacggcgct ggtgtcggtg atcggtctcg
     4321 atgacatcgt gcgcaaggcc cagctcgccg ccggcgccac gcgcctgccg ttcacgttct
     4381 acggcgccgt gctgctcggt tttctggcgt tcacgaccgt ctcggtgctg ctgctgcgct
     4441 gggccgaacg ccgctacagc atcggctacg agcggatgga gtgagcggcg atggaattcg
     4501 gcgtcatcct cgacaactgg ccgctgtacc tcgaaggcct ggtcacgacg gtcgagctga
     4561 ccggtctgtg cctgatcctc ggcctgctgc tcgcgatccc gctcggcacc gtgcaggcgg
     4621 tcgggccgct gtgggcgcgc ggcctggtgt tcgcgtacag ctacgtgttc cgcggcacgc
     4681 cgctgctggt gcagaccttc ctgatctact acggcctcgg ccagttcgag gccgtgcagc
     4741 agagcgtgtt ctggccgctg ctgaaggaag cctactggtg tgcgctgatc gcgttcacgc
     4801 tcaacaccgg cgcctacacc accgagatca tccgcggctc gatcgtgacc atgccgcgcg
     4861 gcgagatcga ggccgcgcgc gcctgcggca tggacacctt gctgatgctg cggcgcatcg
     4921 tgctgccgag cgccttccgc cgcgcgctgc cggcctacgg caacgaggtc atcttcatgc
     4981 tgcagggctc gtcgatcgcc tcgacgatca cgctggtgga cctgaccggg gccgcgcgcg
     5041 tgatcaactc gcgctactac ctgccgtatg aggctttcct gaccgccggc ctgctgtaca
     5101 tggcgctgtc cttcctgctg ttcgcgggct tccgcaagct cgaacagcgc tggttcgcgc
     5161 acctgcggcc gcgggagcat tgaggccccg caaccgtgaa caccgggtct ggcgcccgct
     5221 gcgcgcgccg gccggccccg cttcagtcca gcagcatctc cactgcggcc gcaaagtcat
     5281 cggcgagccc cgcccgcgga cacttgcgct cgtcgccatc ccggtacttg ccggtgcgca
     5341 ccagcaccgc ggcgccgaga ccggcggcga gcgcggcggc gacatcggcc tcggcatcgt
     5401 cgccgatcat cgccacgcgc tgcggcggca ggccgagccg cgcgcagccg gtctcgaaca
     5461 tcgcctgcgc cggcttgccg atgatggtcg cgctgacgcc gctcgcgtat tcgagcgcgg
     5521 cgcggaacgg gccgatgtcg agcgcgagcc gctcgccgtc gcggaagtag cgattgtcgg
     5581 ccatcgccac cagttgcgcg ccgccgagca gcagctgcag cgcatggttc agattctcgt
     5641 agtggaagcc gtcggcggca tcgccgagca cgacgacat
//
LOCUS       DAOQJH010000065         5657 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_8228_length_5657_cov_4.438950, whole genome shotgun sequence.
ACCESSION   DAOQJH010000065 DAOQJH010000000
VERSION     DAOQJH010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 5657)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5657)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5657
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8228_length_5657_cov_4.438950"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            303..1001
                     /locus_tag="PLQ18_02575"
     CDS             303..1001
                     /locus_tag="PLQ18_02575"
                     /inference="COORDINATES: protein motif:HMM:NF014068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HNE00564.1"
                     /translation="MNKRVLIIVTSTPLIDGTGHPTGTWADDIAIPYYALREARCLVD
                     FASPLGGTAPIDPRSLCEVEGALPPSVVQFVADRELQQQLAGTRRVAEIDPLRFDALF
                     VPGGHGALWDLPGDAGVAQVVSSMLGYGQIVATLCHGAAALLGATGYGGRPLVAGRRV
                     TSITDAEESAVGLTAVLPVSLEQRLRELGAEFVHDELWRAYVTRDHALITGQNPQSAP
                     LIARHLVDALRLAG"
     gene            complement(1121..2671)
                     /gene="hutH"
                     /locus_tag="PLQ18_02580"
     CDS             complement(1121..2671)
                     /gene="hutH"
                     /locus_tag="PLQ18_02580"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017413010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HNE00565.1"
                     /translation="MNASPILLRPGALDLALLRAVQEGEPPLCLEPGCRTAIDAAQAT
                     VTRVLAEGRTVYGVNTGFGLLAQTRIAAADLDTLQRSLVRSHAAGVGAPLPESTVRLV
                     LVLKIASLARGHSGVRAELVEALLALYTHGIRPLIPSRGSVGASGDLAPLAHLAAVLI
                     GEGEATYRGVRMSGAAALAAAGLAPLALAPKEGLALLNGTQVSTALALEGLFAAENLL
                     VAALVTGSLSVEAALGSRRPFDARIHAVRGHAAQIDIAAACRALLDHSEIERSHEHCG
                     KVQDPYSLRCQPQVMGACLEQIRHAAAVLLTEANAVSDNPLVFADDDDILSGGNFHAE
                     PVAFAADGLALALAETGALAERRIAMLIDANLSNGLPAFLVAHGGLNSGFMAAQVTAA
                     ALASENKSLAHPASVDSLPTSANQEDHVSMATFAARRLAELAANTAGIVAIEWLAAAQ
                     GVDLRAPHRSSAALRTALALLRAAVPFYERDRCFAPDIARATALIEGGQLRAAVRDAL
                     PAGLLPSG"
     gene            2861..4561
                     /locus_tag="PLQ18_02585"
     CDS             2861..4561
                     /locus_tag="PLQ18_02585"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNE00566.1"
                     /translation="MNEPPVDGARGISALGQRLALTGASDGLWEWNPLTKALYVSPRL
                     LAILGYEQDLLPDTHAWLALIHPEDRDGYNHAVATHLQGRTPYFEHEYRVRAADGAWR
                     WVSARGIAARDAGGRAYCMAGSVADITARKVMEAGMRHLAHHDALTGLPNRTLLEDRF
                     AQALSGARRSGGGLALLFLDLDRFKDVNDAWGHPVGDALLRAVAERLKTSVRAVDTVA
                     RHGGDEFIVVLPATGTPAAAALVARKILAALDAPVEFDAYRLYVRASLGIALYPRDGT
                     DFATLLKCADTALYRAKSAGRGCLRFYTAQMDAELRGRLAIEQGLRTVLSEGGLVLHY
                     QPVFAAGTPGRPLAAEALLRWQRPDGVCVPPAEFIPIAEETGLIVPIGDWVLAEACRQ
                     AQRWHAWIAAGAAIAVNLAVPQLRQPRLPERIAQLLADHGLPPAALEIELTESMLMQR
                     GSASEHVLHRLHAMGLRIAIDDFGTGYSSLAYLRRLPIDTLKIDRSFVAELDTAGGGA
                     IPGAVIALAGQLGMQTTAEGVETLAQAQWLHARGCDRLQGYLHGRPVPADEFERCHLR
                     "
     gene            complement(4577..5230)
                     /locus_tag="PLQ18_02590"
     CDS             complement(4577..5230)
                     /locus_tag="PLQ18_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HNE00567.1"
                     /translation="MPLCTSARCEETVNAVTHGAGFAASLVGLPFLILAAHAREPGGA
                     AAVVGASVFGAAMLLLYGISTVYHAWPETRGKRIWRVLDHAAIYLLIAGTYTPFTLGV
                     LAGPWGWSLFGTVWGLALLGIVLRSFGGLRDCRRCQRLGIALYVAMGWMIVIAAGPMI
                     ERMAVAGLVWLALGGVLYTGGLWFFARDHRRWHHAAWHVFVLGGSVCHYFAVLWHAG"
     gene            complement(5500..>5657)
                     /locus_tag="PLQ18_02595"
     CDS             complement(5500..>5657)
                     /locus_tag="PLQ18_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009156031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HNE00568.1"
                     /translation="EVRKCENPACVLWFRDTSKRGNRRWCSMAACGNRAKAAAHRRRL
                     RAGDGGG"
BASE COUNT          783 a         2049 c         2076 g          749 t
ORIGIN      
        1 tcgcgctcga agccttgcac gcgacgcgaa aagcgtcgcg accggcgccc gcagcctcag
       61 cgccgccgcc ggcagccggt cccggcccgc ggtctggcgc gcgcccgctc ctgcgggcgc
      121 cgggttccgc acggtctccg gagggtgtcc ccgtgcaggc cgctcccggt ggtccctgaa
      181 aaacaaggat cgtcagacat ttgcgtgccg tgatcttgcc ggatgctcat gtgcgcggct
      241 gaaccgctcc cccccggcag ggtcggtccg cacacgggct gagccgtgga caggagcgga
      301 tcatgaacaa gcgtgtgctg atcatcgtca cgtccacgcc gctgatcgac ggcaccggcc
      361 acccgaccgg cacctgggcc gatgacatcg cgattccgta ttacgcgctg cgcgaagccc
      421 gctgcctcgt ggatttcgcc tcgccgctcg gcggaacggc gccgatcgat ccgcgcagcc
      481 tgtgcgaggt cgagggcgcg ctgccgccct cggtcgtgca gttcgtcgcg gaccgcgagc
      541 tgcagcagca gcttgccggg acccggcgcg tcgccgagat cgatccgctg cgcttcgatg
      601 cgctgttcgt gcccggcggc cacggcgcgc tgtgggatct gccgggcgat gccggcgtcg
      661 cgcaggtggt cagctcgatg ctcggctacg gccagatcgt cgcgacgctc tgccacggcg
      721 ccgcggcgct gctcggcgcc accggctacg gcggccggcc gctggtcgcc ggccggcgcg
      781 tgacctcgat caccgatgcc gaggaaagcg cggtcggcct gaccgcggtg ctgccggtgt
      841 cgctggaaca gcgcctgcgc gagctcggcg ccgaattcgt gcacgacgag ctgtggcgcg
      901 cgtacgtgac gcgcgaccac gcgctgatca ccggccagaa cccgcagtcg gcaccgctga
      961 tcgcgcgcca tctggtcgat gcgctgcgcc tcgccggctg aacgcccctg ctggcctcat
     1021 gccagtcaca gctgaacacc cgattgtttc agcccctccc ccctcgcggg ggagggattg
     1081 gggagagggg gatcaacagc gcatgcacgt gtgatcggta tcagccgctc ggcagcagcc
     1141 cggccggcag cgcgtcgcgc accgcggcgc gcagctgccc gccctcgatc agcgccgtgg
     1201 cgcgggcgat gtcgggcgcg aagcagcggt cgcgctcgta gaacggcacc gcggcgcgca
     1261 gcagcgccag cgccgtgcgc agcgctgccg agctgcggtg cggcgcgcgc aagtccacgc
     1321 cctgcgccgc cgccagccat tcgatcgcga cgatgccggc ggtgttggcc gccagctccg
     1381 cgagccggcg cgccgcgaag gtcgccatcg acacgtgatc ctcctgattg gcgctggtcg
     1441 gcaggctgtc gaccgaggcc ggatgcgcga gcgacttgtt ctcggaggcg agtgccgcgg
     1501 ccgtgacctg cgcggccatg aagccggagt tgaggccgcc gtgcgcgacc aggaacgccg
     1561 gcaggccgtt gctcaggttg gcgtcgatca gcatcgcgat gcgccgctcg gcgagcgcgc
     1621 cggtttcggc gagcgcgagc gccagaccat cggccgcgaa cgccaccggc tcggcgtgga
     1681 agttgccgcc ggagaggatg tcgtcgtcgt cggcgaacac cagcgggttg tcggagacgg
     1741 cgttggcctc ggtcagcagc accgcggcgg cgtggcggat ctgttcgagg caggcgccca
     1801 tgacctgcgg ctggcagcgc agcgagtacg ggtcctgcac cttgccgcag tgctcgtgcg
     1861 agcgttcgat ctcgctgtga tcgagcagcg cgcggcaggc ggcggcgatg tcgatctgcg
     1921 ccgcatggcc gcgcacggcg tggatgcgcg catcgaaggg ccggcggctg ccgagcgcgg
     1981 cctcgaccga caggctgccg gtgacgaggg ccgcgacgag caggttctcg gccgcgaaca
     2041 ggccctcgag cgcgagcgcc gtcgatacct gcgtgccgtt gagcagcgcc agcccctcct
     2101 tcggcgccag cgcgagcggc gccagccccg ccgccgccag cgccgcggca ccgctcatgc
     2161 gcacgccccg gtacgtcgcc tcgccctcgc cgatcagcac cgccgccaga tgcgcgagcg
     2221 gcgccagatc gccggacgcg ccgaccgagc cgcgcgacgg gatcagcggc cggatgccgt
     2281 gcgtgtacag cgcgagcagc gcctcgacga gttcggcgcg cacgccggaa tggccgcgcg
     2341 cgaggctcgc gatcttcagc accagcacca ggcgcacggt cgattccggc agcggcgccc
     2401 cgacgccggc ggcgtgcgag cgcaccaggc tgcgctgcag cgtgtcgaga tcggccgctg
     2461 cgatgcgcgt ctgcgcgagc aggccgaagc cggtgttgac gccgtagacc gtgcggcctt
     2521 cggcgagcac gcgcgtgacc gtcgcctggg cagcgtcgat cgccgtccgg cagccgggct
     2581 caagacagag cggcggctcg ccttcctgca cggcgcgcag cagcgccagg tcgagcgcgc
     2641 cggggcgcag caggatcggg gaagcgttca tcgcgcaagg ctccggccgc ggccgacgcg
     2701 actgcccgat ggcgcagccc gcgtgccgcg gcaccgcgaa atagtggcag atgccggtgc
     2761 cgggcacgaa cccgtgccgg acggcgccgg cgatgcggcc tcgggcagat gcgggggctg
     2821 cacccgtgag gctgccgcgg cccggcgata tgctgcggcg atgaacgaac caccggtgga
     2881 cggcgcgcgc ggcatcagcg ccctgggaca gcgtctggcg ctgacgggcg cgagcgacgg
     2941 gctgtgggaa tggaatccgc tgaccaaggc cttgtacgtg tcgccgcgcc tgctcgcgat
     3001 cctcggctac gagcaggatc tgctgcccga cacgcatgcg tggctggccc tgatccaccc
     3061 cgaggatcgc gacggctaca accatgccgt cgccacgcat ctgcaggggc gcacgccgta
     3121 tttcgagcac gagtaccgtg tgcgcgccgc cgacggtgcc tggcgctggg tgtcggcgcg
     3181 cggcatcgcg gcgcgcgacg ccggcggtcg cgcgtactgc atggccggct cggtcgccga
     3241 catcaccgcg cgcaaggtga tggaggcggg gatgcgccac ctcgcgcatc acgatgcact
     3301 gaccggcctg cccaaccgca cgctgctcga agaccgcttc gcgcaggcgc tgtccggtgc
     3361 ccggcgctcg gggggcgggc tggcgctgct gtttctcgac ctcgaccgct tcaaggatgt
     3421 caacgatgcc tggggccacc cggtcggcga tgcgctgctg cgcgcggtgg ccgagcgtct
     3481 gaagacgagc gtgcgcgccg tcgatacggt cgcgcgtcac ggcggcgacg agttcatcgt
     3541 cgtgctgcca gcaaccggaa ccccggccgc tgcggccctc gtcgcgcgca agatcctcgc
     3601 ggcgctcgat gccccggtcg agttcgacgc gtaccggctg tacgtgcgcg caagcctcgg
     3661 catcgcgctg tacccgcgcg atggcacgga cttcgccacg ctgctcaagt gcgccgacac
     3721 ggcgctgtac cgcgccaaga gcgccggccg cggctgtctg cgcttctaca cggcgcagat
     3781 ggatgccgag ctgcgcggcc ggctggcgat cgagcagggc ctgcgcacgg tgctgagcga
     3841 gggcgggctg gtgctgcact accagccggt cttcgccgcc ggcacgcccg ggcggccgct
     3901 cgccgccgag gcgctgctgc gctggcagcg cccggacggt gtctgcgtgc cgccggccga
     3961 attcatcccg atcgccgagg aaaccgggct gatcgtgccg atcggcgact gggtgctcgc
     4021 cgaggcctgc cgtcaggcgc agcgctggca tgcgtggatc gccgccggcg ctgcgatcgc
     4081 cgtcaatctg gccgtgccgc agctgcgtca gccgcggctg cccgagcgca tcgcccagct
     4141 gctggcagac cacgggctgc cgccggcagc gctggagatc gagctgaccg aatcgatgct
     4201 gatgcagcgt ggcagcgcgt ccgagcacgt gctgcaccgc ctgcatgcaa tgggcctgcg
     4261 catcgccatc gacgacttcg gcaccggcta ttcctcgctc gcctacctgc gccggctgcc
     4321 gatcgacacc ctgaagatcg accgcagttt cgtcgccgaa ctcgacacgg ccggcggcgg
     4381 cgccatcccc ggcgcggtca ttgcgctcgc cggccagctc ggcatgcaga ccacggccga
     4441 aggcgtggaa accctcgcgc aggcgcagtg gctgcatgcg cgcggctgcg accggctgca
     4501 gggttacctg cacggccgcc cggtgccggc ggacgaattc gaacgctgcc atctccgctg
     4561 agcgcgggcg gcggcttcag cccgcgtgcc acagcaccgc gaaatagtgg cagacgctgc
     4621 cgccgagcac gaacacgtgc caggcggcgt gatgccagcg ccggtgatcg cgcgcgaaga
     4681 accacaggcc gccggtgtag agcacaccgc cgagcgcgag ccagacgagg ccggcgacgg
     4741 ccatgcgctc gatcatcggc ccggcggcga tgacgatcat ccagcccatc gccacgtaca
     4801 gcgcgatgcc gaggcgctgg cagcggcggc agtcgcgcag gccgccgaag ctgcgcagca
     4861 cgatgccgag cagcgccagc ccccagaccg tgccgaacag cgaccagccc cagggtccgg
     4921 ccagcacgcc gagtgtgaac ggcgtgtagg tgccggcgat cagcaggtag atcgccgcat
     4981 ggtcgagcac gcgccagatg cgcttgccgc gggtttccgg ccaggcgtga tagacggtcg
     5041 agatgccgta gagcagcagc atcgcggcgc cgaagacgct ggcaccaacc acggccgcgg
     5101 cgccgccggg ctcgcgcgcg tgtgcggcaa ggatcaggaa cggcagcccg accaggctgg
     5161 cggcaaagcc ggcgccgtgc gtgacggcgt tgacggtttc ctcgcagcgg gcggaggtac
     5221 agagaggcat ggcggcggcc cccggcgggc cggccggatg ccggcagccc gatgatcggg
     5281 aaggcgcgcc gatgcggcgg ttccgtcacc gtcgcggaag cggcccgggt gcgccggaca
     5341 ggccgattcc cgcagatggt tccgcggcct gctccgcagt ccgcgtgtcc gcaggccgcg
     5401 tccgcggctt gcgggtcagg gcatgggtct gacgtgagtc agacatcggc aacgcgcatg
     5461 tttcctgcag gcccggagcg ccgtcgccgg ccgtcgtcct cacccgccgc cgtcgcccgc
     5521 ccgcaggcgc cggcggtgcg cagcggcctt cgcgcggttg ccgcaggcgg ccatgctgca
     5581 ccagcgccgg ttgccgcgct tgctggtatc gcgaaaccac agcacgcagg ccggattctc
     5641 gcacttgcgc acctcgg
//
LOCUS       DAOQJH010000066         5614 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_8322_length_5614_cov_3.741320, whole genome shotgun sequence.
ACCESSION   DAOQJH010000066 DAOQJH010000000
VERSION     DAOQJH010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 5614)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5614)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5614
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8322_length_5614_cov_3.741320"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..402)
                     /locus_tag="PLQ18_02600"
     CDS             complement(<1..402)
                     /locus_tag="PLQ18_02600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE00569.1"
                     /translation="MSVTDPTPPPAASSREARLAELIELHARDFRGHPYLPEGCAPFT
                     LEAGRVVGHYHGLRLQTTLLALLDAGGRPVARLAQTAAFGAGGQPVSAWAPYAIALDD
                     AAITYLDRLIRTVHVLNAGSRPGGGPLWLAVH"
     gene            763..2691
                     /locus_tag="PLQ18_02605"
     CDS             763..2691
                     /locus_tag="PLQ18_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNE00570.1"
                     /translation="MTDTDRWTEANPDALIGLTAAGVVQHWSAGAQRQYGYPAHEALG
                     RPLEALIVPADMTGQQQWMLAETLALELCAFEAVRRRRDGSLVCVDITARRIAGEDGE
                     SAVLFVEKDVTRLKILRDARLMEARFRDLLESTPDAVVIAGTTGHIVIANAQAERLFG
                     YSPGELRGQPVEVLLPARYRAAHVSHRAGYFQQPRRRSMGAGLELYGLRRDGTEFPVE
                     ISLNQLRTEAGQMVMSAIRDISERQRAERKFRGLLEAAPDAIVIVDRDGRIVLANAQT
                     ATLFGFERRELLGERVERLLPPRFRDRHPAHRNAYFTDPKLRPMGTGFELYGLRRDGT
                     EFPVEISLSPLETEDGLLVSAAIRDITERKRFERALQDKNSELEAANRAKSRFLASMS
                     HELRTPLNAVLGFTGILLMKLPGPLNPEQESQLRTVKSSAQHLLALINELLDLARIEA
                     GRVVLKPEPVALATLLDEVAATLRTQADDKGLALGVELEAVPEPVTDRRLLLQILINL
                     AGNAVKFTQQGSVRLRAGPGRPDSRGHATVCIAVADTGPGISPEEQKRLFEPFAQGEA
                     ARSSGIESSGLGLHLSRELAGVLGGWLQCRSIPGEGSTFTLTLPLHAELPAAADLPGA
                     TAPAELRQPASSGGDRPA"
     gene            2688..3077
                     /locus_tag="PLQ18_02610"
     CDS             2688..3077
                     /locus_tag="PLQ18_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE00571.1"
                     /translation="MNHPNILIVEDNQANFAVLDYLLRAHGFHVLAAGNGREALDVLA
                     REPVDLVLCDMQMPVMDGYEFARCTRANPRYARLPLLAVTASSMAGDRTRVLAAGFDD
                     YFSKPIDPETFVDAMEHHLPPALRRAR"
     gene            3098..>5614
                     /locus_tag="PLQ18_02615"
     CDS             3098..>5614
                     /locus_tag="PLQ18_02615"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNE00572.1"
                     /translation="MARIAVVDDLDFNRDLVAALLRPAGHDIVEAADGAAALEMVRRE
                     RPELIVCDVLMPVMDGYEFVRRLRSDPVCGEYGARVPVIFWSAYYNEDEALHLAAECG
                     VSLLLPKPCPPERLQAVIRQALSEQPPPPLPLPDETGFEGQHRRLLTDKLSEQTTALL
                     RANARLTALLGMVLALGSEHDPQRLLAAACRGARELLGASDAWLVYREDDSAPVRSVS
                     DGPHSALPAPAGFGGSSSQPQDPRFGAVLVAPLSSMRTCRGWLMIARLPGEAPFEDED
                     EHLLGLLAAQTLHAWEIERLNGQLSAHAAALEDEVAERERITRQLDVQYAIARQLAGA
                     QGIDAVLPVLLRTLGERLGFEIAALWLPDARHGGLRCEQVWAAPGSAHLGCAAARIHR
                     RLAPEKGQPGRSRLQRRPLWSLDLHRDEDSGRDPDCPDLHACATLPIAAGERIVAVFE
                     LLAGERRPPDPGLLDLMWAIGEQVGQFWARIDQQHRNARLARVHAVLSGINGALVRIH
                     ERAALLRATCRIAVEQGEFGIAWIGDYDAAAARVTPVAWAGIDDGFGAQALALASSDD
                     DYAALIVREAIHRREAVWCNDLAAEAAPVHSRFAEAQRRGFGSLIVLPLVVDHRIGGV
                     LALYTQAPDYFTREERELLEQVASDIAFGLADIDRRDRLDYLNHYDPLTGLPNRTLWR
                     ERLGHALIESARHHERTAVLVLDIARFRHVNNGFGRAAGDALLRALAERAAAVWPEPA
                     HVGRLGADCFAGFLPRLADVQAITQLLEGRLGAALSAPFPVDGHELRPGFVAGIAIAP
                     DDGDEAETLLHHAEAALAQAKASDRPYVYYRPELTSGIAGT"
BASE COUNT          819 a         2064 c         1957 g          774 t
ORIGIN      
        1 atgcaccgcc agccacagcg gcccgccgcc ggggcggctg ccggcgttca gcacgtgcac
       61 ggtgcggatc agccggtcga gataggtgat cgcggcatcg tcgagcgcga tcgcatacgg
      121 cgcccaggcc gacaccggct ggccgccggc accgaaggcg gcggtctgcg cgaggcgcgc
      181 gaccgggcgc ccgccggcat cgagcagcgc cagcagcgtc gtctgcaggc gcaggccgtg
      241 gtaatgcccg accacgcgcc cggcttcgag cgtgaacggc gcgcagcctt ccggcaggta
      301 gggatggccg cggaagtcac gggcatgcag ttcgatcagc tcggcgagcc gtgcctcgcg
      361 gctggaggct gcggggggcg gggtcggatc ggtgacggac atctgggcgg ctccgctcgg
      421 gatgacaaac atgaaaaatt ggagattcgg agtcagctta cggtcggttt ggcaataata
      481 tatagtaata aaaaattatc tttttattac gcatggttat ttatctgcga catttgactt
      541 atcactgctg cgctgcaacc gaaggatttt ccatcccggc atgccgcctg caaatggcac
      601 tagactcgaa gcgccagacg caggcacgag gcatgacgcg agccggaacc cgcaaccggc
      661 cggaacaggg attccggagt cgggtctgcg ggtgttgcgg attggccggt ggacgggatc
      721 accggcggca tcatgtaccg agacaggccc cccgagatgc agatgaccga taccgatcgc
      781 tggaccgaag ccaaccccga tgcactgatc gggctgacgg cggccggagt ggtgcagcac
      841 tggagcgccg gcgcgcagcg ccagtacgga tatcccgccc acgaggcgct cgggcgcccg
      901 ctggaggcgc tgatcgtgcc ggccgacatg accggccagc agcagtggat gctggccgag
      961 acgctggccc tcgagctctg cgccttcgag gccgtgcgcc ggcgccgcga cggctcgctg
     1021 gtctgcgtcg acatcaccgc gcggcgcatc gccggcgagg acggcgaatc ggcggtgctg
     1081 ttcgtcgaga aggacgtgac gcgcctgaag atcctgcgcg atgcgcggct gatggaggcg
     1141 cgctttcgcg acctgctcga atcgaccccg gatgccgtgg tcatcgccgg caccaccggc
     1201 cacatcgtca tcgccaatgc ccaggccgag cgcctgttcg gctactcgcc cggcgagctg
     1261 cgcggccagc cggtcgaggt gctgctgccg gcacgctacc gcgctgcgca cgtcagccac
     1321 cgcgccggct acttccagca gccgcgccgg cgctcgatgg gcgccgggct cgagctctac
     1381 ggcctgcgcc gcgacggcac cgagttcccg gtcgagatca gcctgaacca gctgcgcacc
     1441 gaggccgggc agatggtcat gagcgcgatc cgcgacatca gcgagcgcca gcgcgccgag
     1501 cgcaagttcc gcggcctgct cgaagccgcg ccggatgcga ttgtcatcgt cgatcgcgac
     1561 ggccgcatcg tgctcgccaa cgcccagacc gcgacgctgt tcggcttcga gcggcgcgag
     1621 ctgctcggcg agcgcgtcga gcgcctgctg ccgccgcgct tccgcgatcg ccacccggcg
     1681 caccgcaacg cctacttcac cgatccgaag ctgcgcccga tgggcaccgg cttcgagctc
     1741 tacgggctgc gccgcgacgg caccgaattc ccggtcgaga tcagcctcag tccgctcgag
     1801 accgaggacg gcctgctggt gtcggcggcg atccgcgaca tcaccgagcg caagcgcttc
     1861 gaacgcgcgc tgcaggacaa gaacagcgag ctcgaagccg ccaaccgtgc caagagccgc
     1921 ttcctcgcca gcatgagcca tgagctgcgc acgccgctca atgccgtgct cggcttcacc
     1981 ggcatcctgc tgatgaagct gcccggcccg ctgaatccgg agcaggaatc gcagctgcgc
     2041 acggtcaagt ccagcgccca gcacctgctc gcgctgatca acgagctgct cgacctggca
     2101 cgcatcgagg ccgggcgcgt ggtgctcaag cccgagccgg tggcgctcgc gacgctgctc
     2161 gacgaggtcg cggcgacgct gcgcacgcag gctgacgaca aggggctcgc cctgggcgtc
     2221 gagctcgaag cggtgcccga acccgtcacc gatcgccggc tgctgctgca gatcctgatc
     2281 aacctcgccg gcaatgccgt caagttcacg cagcagggct cggtgcgcct gcgcgccggc
     2341 cccggccggc ccgattccag ggggcacgcc accgtctgca tcgccgtcgc cgacaccggt
     2401 cccggcatct cgcccgaaga acagaagcgg ctgttcgaac ccttcgcgca gggcgaggcc
     2461 gcccgcagca gcggcatcga gagctccggc ctcggcctgc acctgagccg ggaactggcc
     2521 ggcgtgctcg gcggctggct gcagtgccgc agcatccccg gcgagggcag caccttcacg
     2581 ctgacgctgc cgctgcacgc cgagctgccg gccgccgccg acctgcccgg cgccacggcc
     2641 ccggccgagc ttcgccagcc cgcatcatca ggtggagacc ggcccgcatg aaccatccga
     2701 acatcctgat cgtcgaggac aaccaggcca atttcgccgt gctcgactac ctgctgcggg
     2761 cgcacggctt tcacgtgctc gccgccggca acggccgcga ggcgctcgac gtgctggcgc
     2821 gcgagccggt cgatctggtg ctctgcgaca tgcagatgcc ggtcatggac ggctacgagt
     2881 tcgcgcgctg cacgcgcgcc aacccgcgct acgcgcgcct gccgctgctg gccgtgaccg
     2941 cctcgtcgat ggccggcgat cgcacgcgcg tgctcgcggc cggcttcgat gattacttct
     3001 cgaagccgat cgatccggaa accttcgtcg acgcgatgga gcaccacctg ccgccggcac
     3061 tgcgacgggc gcgttgacgg gcgcgggcgg gcggccgatg gcgcgcattg cagtcgtcga
     3121 tgatctcgac ttcaaccgcg acctggtcgc ggcgctgctg cgcccggccg ggcacgacat
     3181 cgtcgaggcc gccgatggtg cggcagccct ggagatggtc cggcgcgagc gcccggaact
     3241 gatcgtctgc gacgtgctga tgccggtcat ggacggctac gagttcgtgc gccggctgcg
     3301 ctccgatccg gtctgcggcg aatacggcgc gcgcgtgccg gtgatcttct ggagcgcgta
     3361 ttacaacgag gacgaggccc tgcacctggc cgccgaatgc ggcgtcagcc tgctgctgcc
     3421 caagccctgc ccgcccgagc gcctgcaggc ggtgatccgg caggcgctca gcgagcagcc
     3481 gccgccgccg ctgccgctgc ccgatgaaac cggcttcgag gggcagcacc ggcggctgct
     3541 gaccgacaag ctctccgaac agaccacggc gctgctgcgc gccaatgccc gcctgaccgc
     3601 gctgctcggc atggtgctcg cgctcggcag cgaacacgat ccgcagcgcc tgctcgccgc
     3661 cgcctgccgg ggcgcccgcg aactgctcgg cgcgagcgac gcctggctgg tgtaccgcga
     3721 ggacgacagc gccccggtgc gctcggtcag cgacggtccg cactcggcgc tgccggcacc
     3781 ggccggtttc ggcggcagca gcagccagcc gcaggatccg cgcttcggcg cggtgctggt
     3841 cgccccgctc agctcgatgc gcacctgccg cggctggctg atgatcgccc gcctgccggg
     3901 cgaggcgccg ttcgaggacg aggacgaaca cctgctcggc ctgctcgcgg cgcagacgct
     3961 gcatgcctgg gagatcgagc gcctgaacgg ccagctctcg gcgcatgccg ccgcgctcga
     4021 ggacgaggtc gccgagcgcg agcgcatcac ccgccagctc gacgtgcagt acgcgatcgc
     4081 ccgtcagctc gccggtgcgc agggcatcga tgccgtgctg ccggtgctgc tgcgcacgct
     4141 cggcgagcgc ctcggtttcg agatcgccgc gctgtggctg cccgatgccc gccacggcgg
     4201 cctgcgctgc gagcaggtct gggccgcgcc cggctcggcg cacctcggct gcgccgcggc
     4261 ccgcatccac cggcggctcg cgccggagaa aggccagccg gggcgcagcc gcctgcagcg
     4321 ccggccgctc tggagcctcg acctgcaccg cgacgaggac agcggccgcg atccggactg
     4381 ccctgacctg cacgcctgcg cgacgctgcc gatcgccgcc ggcgagcgca tcgtcgcggt
     4441 gttcgagctg ctggccggcg agcgccggcc gccggacccg ggtctgctcg acctgatgtg
     4501 ggcgatcggc gagcaggtcg gccagttctg ggcccgcatc gaccagcagc atcgcaacgc
     4561 ccgcctcgcg cgcgtgcacg cggtgctgag cggcatcaac ggcgcgctgg tgcgcatcca
     4621 cgagcgcgcg gcgctgctgc gcgccacctg ccgcatcgcc gtcgaacagg gcgagttcgg
     4681 catcgcctgg atcggtgact acgacgccgc tgccgcacgg gtgacgccgg tggcctgggc
     4741 cggcatcgat gacggtttcg gcgcgcaggc gctcgcgctc gccagcagcg acgatgacta
     4801 cgcggcgctg atcgtgcgcg aggccatcca ccggcgcgag gcggtctggt gcaatgatct
     4861 cgccgccgag gccgcaccgg tgcacagccg cttcgccgag gcgcagcgcc gcggcttcgg
     4921 ctcgctgatc gtgctgccgc tggtcgtcga tcaccgcatc ggcggcgtgc tggcgctgta
     4981 cacgcaggcg ccggactact tcacgcgcga ggaacgcgag ctgctcgaac aggtcgcgag
     5041 cgacatcgcc ttcggcctcg ccgacatcga ccgccgcgac cggctcgact acctgaacca
     5101 ctacgatccg ctgaccggcc tgccgaaccg cacgctgtgg cgcgagcggc tcgggcacgc
     5161 gctgatcgag agcgcgcgcc accacgagcg cacggccgtg ctcgtgctcg acatcgcccg
     5221 cttccggcac gtcaacaacg gcttcggccg cgccgccggc gatgcgctgc tgcgcgcgct
     5281 cgccgagcgc gccgcggcgg tctggcccga gcccgcgcac gtcgggcggc tcggcgccga
     5341 ctgcttcgcc ggcttcctgc cgcggctcgc cgatgtgcag gcgatcacgc agctgctcga
     5401 aggccggctc ggtgccgcgc tgtcggcacc gttcccggtc gacggtcacg agctgcgccc
     5461 gggcttcgtc gccggcatcg ccatcgcccc ggacgacggc gacgaggccg aaaccctgct
     5521 gcaccacgcc gaggcggcgc tggcgcaggc caaggccagc gaccggccct acgtgtacta
     5581 ccgccccgag ctgaccagcg gcatcgccgg cacg
//
LOCUS       DAOQJH010000067         5588 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_8386_length_5588_cov_4.214531, whole genome shotgun sequence.
ACCESSION   DAOQJH010000067 DAOQJH010000000
VERSION     DAOQJH010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 5588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5588
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8386_length_5588_cov_4.214531"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..156)
                     /locus_tag="PLQ18_02620"
     CDS             complement(<1..156)
                     /locus_tag="PLQ18_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004208059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNE00573.1"
                     /translation="MRTVTLGCTDLVVSRACLGTMTWGEQNTEAEARAQLDFATARGV
                     NFIDTAEM"
     gene            complement(278..967)
                     /gene="trmB"
                     /locus_tag="PLQ18_02625"
     CDS             complement(278..967)
                     /gene="trmB"
                     /locus_tag="PLQ18_02625"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008283891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HNE00574.1"
                     /translation="MNDVENPRRIRSFVRREGRLTLAQQRALDELWPRFGIDFSPAAL
                     DLQSLFGRTALRVLEIGFGNGESLAAMAAAAPYTDFIGIEVHRPGVGHLLLAIERLAL
                     QNLRVCCHDAVEVLERQIPDASLDRVHIFFPDPWHKKRHHKRRLIQPPFVALLARKLK
                     PGGHLHLATDWEDYAVHMLAVLGQSADFANSAPAGEMFCPRPDYRPLTKFERRGERLG
                     HGVRDLIFERT"
     gene            complement(1037..1846)
                     /locus_tag="PLQ18_02630"
     CDS             complement(1037..1846)
                     /locus_tag="PLQ18_02630"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008933072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HNE00575.1"
                     /translation="MSASTDQTASTPAPLVIAGREYRSRLLVGTGKYRDLDETRRATE
                     ASGAEIVTVAIRRTNIGQTPGEPNLLDVLPPDRYTILPNTAGCFDADSAVRTCRLARE
                     LLDGHDLVKLEVLGDQKTLFPDIVETLKAAEILIRDGFKVMVYTNDDPLIAKRLETMG
                     CVAVMPLAAPIGSGLGVRNPYNILTIVENAQVPVLVDAGVGTASDAAIAMELGCDGVL
                     MNTAIAGARNPVLMASAMKKAVEAGREAWLAGRIPKKRFASASSPLDGLFF"
     gene            complement(1921..2121)
                     /gene="thiS"
                     /locus_tag="PLQ18_02635"
     CDS             complement(1921..2121)
                     /gene="thiS"
                     /locus_tag="PLQ18_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003297182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HNE00576.1"
                     /translation="MQIKVNGQPYEAEDSLTAAALVERLGYAGRRLALEINREIVPRS
                     RWSQTPLQADDEVELVHAIGGG"
     gene            2608..2961
                     /locus_tag="PLQ18_02640"
     CDS             2608..2961
                     /locus_tag="PLQ18_02640"
                     /inference="COORDINATES: protein motif:HMM:NF013014.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H-NS histone family protein"
                     /protein_id="HNE00577.1"
                     /translation="MSVRFEDIDVSTFNLEQLQALMEMARKEIEVKEQTRLLEVAAQI
                     QSLASGVGRSVEEILSMSSDGKRKRTSKSDTGEKIVKFRNTVNPDETWSGRGKRPRWL
                     QEALLNGADIQSFAV"
     gene            3014..3087
                     /locus_tag="PLQ18_02645"
     tRNA            3014..3087
                     /locus_tag="PLQ18_02645"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3046..3048,aa:Gly,seq:ccc)
     gene            3246..4049
                     /gene="dapB"
                     /locus_tag="PLQ18_02650"
     CDS             3246..4049
                     /gene="dapB"
                     /locus_tag="PLQ18_02650"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016489177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HNE00578.1"
                     /translation="MIRIAIAGVAGRMGRQLVEACAQAADVTLAVAVERPGSSMIGCD
                     AGELAGIGRNGVVVGDDLAARLDDFDVLIDFTRPEPTLAHLALCRAAGRCAVIGTTGF
                     TAAQKAEIQAAAADAGIVFAPNFSVGVNLCFRLLDLAARVLGDEVDIEVLEAHHRHKV
                     DAPSGTALRMGEVVAQALGRELSEVAVYGREGQTGERDRRTIGFATVRAGDIVGEHTV
                     LFADIGERVEITHKASSRMTFARGAVRAAGWLAGRAPGLYDMQDVLGLR"
     gene            4207..5343
                     /gene="carA"
                     /locus_tag="PLQ18_02655"
     CDS             4207..5343
                     /gene="carA"
                     /locus_tag="PLQ18_02655"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015279318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HNE00579.1"
                     /translation="MLRKPALLALADGSLFRGESIGADGSTTGEVVFNTSITGYQEIL
                     TDPSYCRQIVTLTYPHIGNVGANPGDEEAAKIWSSGLVVRDCPPRPSSWRSRQSLDAY
                     LSERGVVAIAGIDTRRLTRHLREQGAQNGCIVAGDRPDAEAAIAAARAAPKMVGLDLA
                     QVVTTESRYEWTEGCWRLDGDDFPQQAEARFHVVAYDYGVKRNILRMLAARGCRVSVV
                     PARTPAAEVLALRPDGVFLSNGPGDPEPCDYAITAIRELLDAGVPIFGICLGHQLLGL
                     ASGARTVKMKFGHHGGNHPVQDLDSGRVMISSQNHGFAVDEATLPATLRATHRSLFDG
                     TLQGIERTDRPAFSFQGHPEASPGPHDVAGLFDRFIGLMQRHGR"
BASE COUNT          944 a         1825 c         1911 g          908 t
ORIGIN      
        1 catctcggcg gtgtcgatga agttcacgcc gcgggccgtc gcgaaatcga gctgcgcgcg
       61 ggcttcggcc tcggtgttct gctcgcccca ggtcatcgtg ccgagacagg cgcggctgac
      121 gacgagatcg gtgcaaccca gggtcacggt gcgcatgcgc ggaggctccg gcggtcggga
      181 tcggaagccg gggaacgtaa cccgcaggag gtatcgatcc ccatgcctgc atgcgtggca
      241 tgcacggagc gacgggccgg gccgcggccg gtgctgctca ggtccgctcg aagatcagat
      301 cccgaacccc gtggccgagc cgctcgccgc ggcgctcgaa tttggtcagc ggccggtaat
      361 ccgggcgtgg acagaacatt tcacctgcgg gcgcggaatt ggcgaaatcc gccgactggc
      421 cgagcacggc gagcatgtgg acggcgtaat cctcccagtc ggtggccagg tgcagatggc
      481 cgccgggctt gagtttgcgg gccagcagcg ccacgaacgg cggctggatc agccggcgtt
      541 tgtggtggcg cttcttgtgc cagggatcgg gaaagaaaat gtggacgcga tcgagacttg
      601 catccggaat ctgtcgttcc agcacctcga cggcgtcatg gcagcaaacc cgcaggttct
      661 gcagggccag gcgttcgatc gcgagcagca ggtgtccgac cccgggccgg tggacctcga
      721 tgccgatgaa gtcggtatag ggcgccgccg ccgccatcgc cgccagcgat tccccgttgc
      781 cgaaaccgat ttccagcacg cgcagcgccg tgcggccgaa cagggattgc aggtcgagtg
      841 ccgcaggact gaagtcgatg ccgaatcgcg gccagagttc atcgagggca cgttgctgcg
      901 cgagagtcag ccggccttcc cggcgcacga aactgcggat gcgccgggga ttttcgacgt
      961 cgttcatgat cggttgggag ggtagaaaac ttttttgcag gcgccgccgc cgcgatgggc
     1021 gcagcggcgg cggggatcag aaaaacaggc catcgagcgg cgaggatgcg ctcgcgaaac
     1081 gctttttcgg aatgcgtccg gccagccagg cctcgcgacc ggcttcgacg gcttttttca
     1141 tcgcactcgc catcagcacc ggattgcggg ctccggcgat cgcggtgttc atcaatacgc
     1201 cgtcgcagcc gagttccatc gcgatcgccg catccgaggc cgtgccgacg ccggcatcga
     1261 ccagcaccgg cacctgtgca ttctcgacga tggtcaggat gttatacgga ttgcgcacgc
     1321 cgaggcccga accgatcggc gcggccagcg gcatcaccgc cacgcagccc atggtttcga
     1381 ggcgcttcgc gatcagcggg tcgtcattcg tgtagaccat gaccttgaag ccgtcgcgaa
     1441 tcaggatttc cgcggccttc agggtttcga cgatgtccgg aaacagcgtc ttctgatcgc
     1501 cgagcacttc gagcttgacc agatcgtggc catcgagcag ctcgcgcgcg agccggcagg
     1561 tgcgcacggc gctgtcggcg tcgaagcagc cggcggtgtt cggcaggatc gtgtagcgat
     1621 ccggcggcag cacgtcgagc aggttcggct cgccgggcgt ctggccgatg ttggtgcgcc
     1681 ggatcgcgac cgtgacgatt tcggcgccgc tcgcctcggt tgcccgccgg gtctcgtcca
     1741 gatcgcggta tttgccggtg ccgaccagca ggcgcgagcg gtattcgcga ccggcaatca
     1801 ccagcggggc aggggtggaa gcggtttgat cggtacttgc ggacatgggc ggactcgggc
     1861 gcagtgagga cgcggtgagg aagggacgga ttgcggccgc aggacgggac gggcgtcgat
     1921 tcagccgcca ccgatcgcgt ggacgagttc gacctcgtca tccgcctgca gcggcgtctg
     1981 cgaccagcgg ctgcgtggca cgatttcgcg attgatctcc agtgccagac gcctgccggc
     2041 gtatccgagg cgttcgacga gcgcggccgc cgtcagggaa tcttcggctt cgtaaggctg
     2101 gccgttgacc ttgatctgca tgatgaggaa tgcatccggt cgggcaggga cggggcggcg
     2161 atcatacacg atcgcgcgtg cctgacagta caagtgcggg ccgctgccgg caatcgcggc
     2221 tgccaagcaa ggggccatgg acggtaacga cctgatgcat cgaagtgaaa tgcagaccgt
     2281 gacaacggct tgcggttccc ggcacccggc gactgcgaca atgccggtgt cgccgaagta
     2341 ccggatcaca aagccgatgg ctgcacttac gcatgacagc cgccccggca ggtgcccgaa
     2401 tgtgtcggcg gtcagccgtg gcgttacacg gacacgctaa tgcagcatga aatttagcag
     2461 ggtcgatact ggcaagtccg atattcatga attaccatgc ctgacatccg cagtgtcgga
     2521 ttgatccgga agcagccgct acgtgacccg ccaggacgag cctgctcccg caacatttac
     2581 gatgcaccca gccgagagat cattcccatg agcgtacgct tcgaagacat cgatgtttcg
     2641 accttcaacc tcgaacaact gcaggcgctg atggagatgg cgcgcaagga aattgaagtc
     2701 aaggagcaga cccggctgct ggaagtcgct gcgcagattc agagtctggc cagcggcgtc
     2761 ggccgcagcg tcgaggaaat cctgtcgatg tccagcgatg gcaagcgcaa gcgcaccagc
     2821 aagtcggata ccggtgaaaa aatcgtcaag ttccgcaaca ccgtgaatcc cgacgaaacc
     2881 tggagcggtc gcggcaagcg cccgcgctgg ctgcaggaag ccctgctgaa cggtgccgac
     2941 atccagagtt ttgccgtctg agcaggcccg gggcttgccg aatgccgtac aggcggcata
     3001 ataggcgggc ccagcgggtg tagttcaatg gcagaacctc agcttcccaa gctgatggcg
     3061 tgggttcgat tcccatcacc cgctccagaa ccgcaagggc cgcgccagtc gcggcccttt
     3121 tcatttccga gcatcgcgcg cgccgccgtc cgtgaattcg gggcacccgg ggcagccagg
     3181 ggcaagcttc tgaatcatcg cgaatccgga ccggctgcgg gatgccagat cgaggccgcc
     3241 agcccatgat cagaattgcg attgccggcg tcgccgggcg catggggcgc cagctcgtcg
     3301 aggcctgtgc gcaggctgcg gacgtgacgc tcgccgtcgc cgtcgagcgg cccggcagca
     3361 gcatgatcgg ctgcgatgcc ggggaactcg ccggcatcgg gcgcaacggc gtcgtcgtcg
     3421 gtgacgacct tgcggcccgg ctcgatgact tcgacgtgct gatcgacttc acgcgcccgg
     3481 agccgacgct cgcgcatctg gcgctctgtc gcgcggccgg gcgctgcgcc gtgatcggca
     3541 cgaccggctt cacggcggcg cagaaggcgg agatccaggc cgccgccgcc gatgccggca
     3601 tcgtgttcgc gccgaacttc agcgtcggtg tgaatctatg tttcaggctg ctcgacctcg
     3661 ctgcccgcgt gctcggcgat gaggtcgata tcgaagtgct cgaagcccat caccggcaca
     3721 aggtcgatgc gccctcgggc acggcgctgc gcatgggcga ggtggtcgcg caggcgctcg
     3781 ggcgcgagct gtccgaagtc gccgtatacg gccgcgaggg ccagaccggc gagcgcgacc
     3841 ggcgcacgat cggcttcgcg accgtgcgcg ccggcgacat cgtcggcgag cacacggtgc
     3901 tgttcgccga catcggcgag cgtgtcgaga tcacgcacaa ggcgtcgagc cgcatgacct
     3961 tcgcgcgcgg tgcggtgcgc gccgccggct ggctggccgg gcgcgctccg gggctctacg
     4021 acatgcagga cgtgctcggc ctgcgctgag ccgggcaccg tcgtcatgcg tcgtcatgga
     4081 cggaattgct gccctgcggt agatttccgg cactttgact gagattgacg aaaacgtaat
     4141 cctcggacac cgggcgggag ggaaggcaac gtttccctcc cgccttcgca cgtctgaatg
     4201 gaggaaattt tgaggaaacc cgcactgctg gccctggcag acggcagcct gttccggggt
     4261 gagtccatcg gtgccgatgg ttcgaccacc ggcgaagtcg tgttcaacac ctcgatcacc
     4321 ggctatcagg agatcctcac cgacccctcg tactgccggc agatcgtgac gctgacctat
     4381 ccgcacatcg gcaacgtcgg tgccaatccc ggcgacgagg aggcggcgaa gatctggtcc
     4441 tcggggctgg tcgtgcgcga ctgcccgccg cgtccgagca gctggcgcag ccgccagtcg
     4501 ctcgatgcct atctgagcga gcgcggcgtg gtcgcgatcg ccggcatcga cacgcgccgg
     4561 ctgacgcgcc acctgcgcga gcagggcgcg cagaacggct gcatcgtcgc cggcgaccgg
     4621 ccggatgccg aggccgcgat cgccgcggcg cgcgccgcgc cgaagatggt cgggctcgac
     4681 ctcgcgcagg tggtgacgac cgaaagccgc tacgaatgga ccgaaggctg ctggcggctc
     4741 gacggcgatg acttcccgca gcaggccgag gcgcgcttcc acgtcgtcgc ctacgactac
     4801 ggcgtcaagc gcaacatcct gcgcatgctc gccgcgcgcg gctgtcgcgt cagcgtcgtg
     4861 ccggcgcgca cgccggcggc cgaggtgctg gccctgcgcc ccgatggcgt gttcctgtcc
     4921 aacggtccgg gcgatcccga gccctgcgac tacgcgatca cggcgatccg cgagctgctc
     4981 gacgccggcg tgccgatctt cggcatctgt ctcggccacc agctgctcgg actggcctcc
     5041 ggcgcgcgca cggtcaagat gaagttcggc caccacggcg gcaatcatcc ggtgcaggat
     5101 ctcgattccg gccgcgtgat gatctccagc cagaaccacg gcttcgcggt cgatgaggcc
     5161 acgctgccgg ccacgctgcg cgccacgcac cgttcgctgt tcgacggcac gctgcagggc
     5221 atcgagcgca ccgaccggcc ggcgttcagc ttccagggcc atccggaagc gagtcccggg
     5281 ccgcacgacg ttgccggcct gttcgaccgc ttcatcggcc tgatgcaacg ccacggccgc
     5341 tgagcctgcc tgaggccggc gcccgtgcgt gcgcacgggg cgccggcacg cccgaatctc
     5401 ccgaacgcct cgctgcgtct gctgcccacg gtttccgtcc atgccgaaac gtaccgacat
     5461 ccagagcatc ctgatcatcg gcgccggtcc catcgtgatc ggccaggcct gcgagttcga
     5521 ctactcgggc gcccaggcct gcaaggcgct gcgcgaggag ggctaccgcg tcatcctcgt
     5581 caacagca
//
LOCUS       DAOQJH010000068         5534 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_8502_length_5534_cov_5.244570, whole genome shotgun sequence.
ACCESSION   DAOQJH010000068 DAOQJH010000000
VERSION     DAOQJH010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 5534)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5534)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5534
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8502_length_5534_cov_5.244570"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            22..1602
                     /gene="ahpF"
                     /locus_tag="PLQ18_02660"
     CDS             22..1602
                     /gene="ahpF"
                     /locus_tag="PLQ18_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012052903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HNE00580.1"
                     /translation="MLDNTLKTQLKAYLERIVQPVTLSASLDDGDKSREMRGLLEDIA
                     ALSGGRVTLETASAAAAGVHVPSFTVGRTAAAGSAGIRFAGIPLGHEFTSLVLALLQT
                     GGHPPKVAPELIEQIRALPGEYRFQTYISLSCHNCPDVVQALNLMAVLNPRVTHEMID
                     GALFQDEVERLSIMAVPTVLLNGETFGQGRMSLEEIVAKLDSGAGARAADRLNAKEPF
                     DVLVVGGGPAGAAAAIYAARKGIRTGVAAERFGGQVLDTLGIENFISVKETEGPKLAA
                     ALEEHVRSYEVDIMNLQRATKLVPGTDGVPAEVELASGARLKAKTVILATGARWRDMN
                     VPGEQEYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVAHVTLLEFAP
                     ELKADAVLQRKLHSLPNVTVIVQAQTTEVTGDGQKVSGLRYTDRASGEAKRIELEGIF
                     VQIGLLPNTDWLRGTVELSRFGEIIVDAKGQTSVPGVYGAGDCTTVPYKQIVIAMGQG
                     STAALAAFDHLIRTSAPA"
     gene            complement(1747..2220)
                     /locus_tag="PLQ18_02665"
     CDS             complement(1747..2220)
                     /locus_tag="PLQ18_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009505793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HNE00581.1"
                     /translation="MPHAALDRYDLKILGVLQREGRIPNNELADRVGLTPSPCLRRVR
                     ALEEAGVIRRYVGLVEPGAVGLPISVFVHISLTSQERASLETFETAVRAFPEVMECYL
                     MTGVADYLVRVVVPDLDAYERFLLDRLTRVPGVTNIRSSFALKQVIYKTELPLGD"
     gene            complement(2307..2708)
                     /locus_tag="PLQ18_02670"
     CDS             complement(2307..2708)
                     /locus_tag="PLQ18_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNE00582.1"
                     /translation="MRYLLDTNICIYISKCRPLLVRQRFENIESAAVGMSVITWGELC
                     FGARKSQSSQRAMEVLEQLQSMIRVVPITTEVGEHYGDIRASLERGGRPIGNNDLWIA
                     AHARALACTLVTNNEREFVRVPDLSVENWLN"
     gene            complement(2714..2956)
                     /locus_tag="PLQ18_02675"
     CDS             complement(2714..2956)
                     /locus_tag="PLQ18_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HNE00583.1"
                     /translation="MAIARIFKSGNSQALRLPKEFRFAGDRVEILRRGEEIVLRELPV
                     SAAAIFDALAALPDDLFAEGRPDGPDDMPQDREPLL"
     gene            3176..>5534
                     /gene="aceE"
                     /locus_tag="PLQ18_02680"
     CDS             3176..>5534
                     /gene="aceE"
                     /locus_tag="PLQ18_02680"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007228974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HNE00584.1"
                     /translation="MHASAQPQPSAAAAEIPGSASDRAALEADRRDWIDSLAGLHAAH
                     GPAGVREILQSLQQYALAHELAPVEAVLNTPYRNTIAVSAQAPYPGDAELEARIANIN
                     RWNAMAMVLQAADSGSGVGGHIATYLSAATLLEVGFNHAFRARSADYGGDLLICQPHA
                     APGVYARAFLEGRLNAAQLGNFRRELGAGGGLPSYPHPRRLPDFWQAPCASMGLSTPT
                     AIHQARFRKYLENRGLAPAGGGGRVWCFIGDGEADEPEVLGTIGIAARERLDNLVLVV
                     NCNLQRLDGPVRGNGKIIQELERSFRGAGWNVLKVIWGSGWDPLFARDRDGSLIRRME
                     SAVDGDYQRYTVLPGEAVREHWVLDNPALAELMASLSDEEIRSIKRGGQDPLKIWAAY
                     AQAMQPNGRPTVILAKTVKGDGMGPGGEGRNTAHQKKQLSADERRDCARRWGIPLADE
                     DIVRAAFYRPAADSPELRYLHARRERLGGYLPARRVDCPPLPAPAAATLAESFEGSGE
                     RALSTTTAAVRLLGRLLRDPALGRLIVPIVPDEARTFGMEGLFRSAGIYSPEGQRYVP
                     VDGDTLLPYREARDGQILQEGICEAGAMASFLAAGTAYAEHGVPSIPFYFFYSMFGSQ
                     RVGDLVWACGDMLARGFLLGGTAGRTTLNGEGLQHQDGHSPLLAATVPNLKVYDPAWG
                     WELATIVEDGIERMYGRGEAVFYYLTVYNEAYPMPARPADPAAREGVLRGLYRLREPA
                     LPGAGPVRVNLFGSGSLLPQALAAAELLEARGFATAVWSATSYVEL"
BASE COUNT          906 a         1950 c         1857 g          821 t
ORIGIN      
        1 cgatcgacgg aagccgcagc catgctcgac aacaccctca agacgcagtt gaaagcctac
       61 ctcgaacgca tcgtgcagcc ggtgacgctg agcgccagcc tcgatgacgg cgacaaatcc
      121 cgcgagatgc gcggtctgct cgaagacatc gccgcgctgt ccggcggccg cgtgacgctc
      181 gaaaccgcga gtgcggcagc cgccggcgtg cacgtgccgt cgttcacggt cgggcgcacc
      241 gccgccgccg gcagcgccgg catccgtttc gccggcatcc cgctcggcca cgaattcacg
      301 tcgctggtgc tcgcgctgct gcagaccggc ggccacccgc cgaaggtcgc gcccgagctg
      361 atcgagcaga tccgcgcgct gcccggcgaa taccgcttcc agacctacat ctcgctgtcc
      421 tgccacaact gcccggatgt cgtgcaggcg ctgaacctga tggcggtgct gaacccgcgc
      481 gtcacgcacg agatgatcga cggtgcgctg ttccaggacg aggtcgaacg cctctcgatc
      541 atggccgtgc cgaccgtact gctgaacggc gagaccttcg gccagggccg catgagcctc
      601 gaagagatcg tcgcgaagct cgacagcggc gccggtgccc gtgcggccga tcgcctgaac
      661 gccaaggagc ccttcgatgt gctcgtcgtc ggcggcggcc cggccggggc ggcggcggcg
      721 atctacgccg cgcgcaaggg catccgcacc ggggtcgcgg ccgagcgctt cggcggtcag
      781 gtgctcgaca cgctcggcat cgagaacttc atctcggtca aggaaaccga aggcccgaag
      841 ctcgccgccg cgctcgagga gcacgtgcgc agctacgagg tcgacatcat gaacctgcag
      901 cgcgcgacga agctcgtgcc cggcactgac ggcgtcccgg ccgaggtcga gcttgcgagc
      961 ggggcgcggc tgaaggcgaa gaccgtgatc ctcgccaccg gcgcgcgctg gcgcgacatg
     1021 aacgtgccgg gcgagcagga ataccgcacg aagggcgtga cctactgccc gcactgcgac
     1081 ggcccgctgt tcaagggcaa gcgcgtggcg gtgatcggcg gcggcaactc cggcgtcgag
     1141 gcggcgatcg atctcgccgg catcgtcgcg cacgtgacgc tgctcgaatt cgcgccggag
     1201 ctgaaggccg atgccgtgct gcagcgcaag ctccacagcc tgccgaacgt cacggtgatc
     1261 gtgcaggcgc agaccaccga ggtcaccggc gacggccaga aggtcagcgg cctccggtac
     1321 accgaccgcg ccagcggcga ggcgaagcgc atcgagctcg aaggcatctt cgtgcagatc
     1381 ggcctgctgc cgaacaccga ctggctgcgc ggcacggtgg agctgtcgcg cttcggcgag
     1441 atcatcgtcg atgcgaaggg ccagacctcg gtgcccggcg tctacggtgc cggcgactgc
     1501 acgacggtgc cgtacaagca gatcgtcatc gccatgggtc agggttcgac cgccgcgctc
     1561 gcggccttcg accacctgat ccgcacctcg gccccggcct gaaccttccc gcccccgccg
     1621 ccgccgcgcg gcggggagcg atccggtttc cgccccccgc ggccggtgca ggtgttccac
     1681 gtgaatcctg tgccggtgcg ggggtttttt tccgtcgcgg cttcgtttgc gccgtcgttt
     1741 gcgcgttcag tcgccgagcg gcagttcggt cttgtagatg acctgcttca gcgcaaagct
     1801 cgaacggatg ttggtcacgc ccggcacgcg cgtcagccgg tcgagcagga agcgttcgta
     1861 ggcatcgaga tcgggcacga ccacgcgcac caggtaatcg gcgacgccgg tcatcagata
     1921 gcactccatg acctccggaa atgcgcgcac ggcggtctcg aaggtttcga ggctggcgcg
     1981 ctcctggctg gtcagcgaga tgtgcacgaa cacgctgatc ggcagcccga ccgcccccgg
     2041 ctcgaccagc ccgacgtagc ggcggatcac cccggcctcc tccagcgcac gcacgcggcg
     2101 caggcacggc gacggcgtca ggccgacgcg atcggccagc tcgttgttcg ggatgcggcc
     2161 ttcgcgctgc agcacgccga ggatcttcag gtcgtagcga tcgagtgcgg catgcggcat
     2221 gttctgtctc cccggtatcg ttatccggca gagtgttgcg catcgacgca gcaaaatccg
     2281 catccgcttg caaaaagccg taccggtcag ttcaaccagt tttcgaccga aagatcagga
     2341 acacgcacga actcgcgctc attattggta acgagcgtgc atgccagtgc ccgcgcatgt
     2401 gcggcaatcc atagatcgtt gttaccgatc ggccggcccc cacgctccag cgaggcgcgg
     2461 atgtcgccgt agtgttcgcc aacctctgtc gtgatcggca cgacccggat catggactga
     2521 agctgttcga gcacttccat ggcacgttgg gatgactggc tcttgcgagc cccgaagcaa
     2581 agctcccccc aggtgatgac cgacatgccc actgcggcag attcgatgtt ctcgaatcgc
     2641 tgccgaacaa gcagaggtct gcatttgctg atgtagatac agatgttggt atcgagcaga
     2701 taacgcagat cgttcacagc aggggttccc ggtcctgcgg catatcgtcc ggaccatccg
     2761 ggcgaccttc tgcaaacaga tcgtcaggca gcgccgcgag cgcatcaaaa atggctgcgg
     2821 cactgaccgg caattcacgc agcacgattt cttcaccccg gcgaaggatt tcgacccgat
     2881 cgccggcaaa cctgaactcc ttgggcagac gcaaggcctg gctgttgccg gatttgaaaa
     2941 tgcgtgcgat ggccatcgtg gtcccctcga aggatatacg caaagtatat acatcctgtg
     3001 caagcctcct tcacacgccc ggaagcaaga acaacaaatc ccgcaatttc ccatcgaaac
     3061 agcatctttg ttgcgcagga caaggtcaca tcacccacat acgcaatcgc ccgccctgcc
     3121 cgcctcccta gactggccgc catcccgcca ccattgccca ggagaggccg ccgccatgca
     3181 tgcgtccgcc cagccccagc cgtccgcggc tgcagcagaa atccccggca gcgcgtccga
     3241 tcgcgccgcc ctcgaagccg atcgccgtga ctggatcgat tcgctcgccg gcctgcacgc
     3301 cgcacacggg ccggccggcg tgcgcgagat cctgcagagc ctgcagcagt acgcgctcgc
     3361 gcacgagctg gcgccggtcg aggcggtgct gaacacgccg taccgcaaca cgattgccgt
     3421 gagcgcacag gcgccgtatc cgggcgatgc cgagctcgaa gcgcgcatcg ccaacatcaa
     3481 ccgctggaac gcgatggcga tggtgctgca ggcggccgac agcggcagcg gcgtcggcgg
     3541 ccacatcgcc acctacctgt cggcggcgac gctgctcgaa gtcggcttca accacgcttt
     3601 ccgcgcgcgc agcgccgact acggcggtga cctgctgatc tgccagccgc atgccgcgcc
     3661 cggcgtctac gcgcgcgcct tcctcgaagg ccggctgaac gccgcgcagc tcggcaactt
     3721 ccgccgcgaa ctcggcgcgg gcggcggcct gccctcgtat ccgcatccgc gccggctgcc
     3781 ggacttctgg caggcgccgt gcgcatcgat gggcctgtcg acgccgaccg cgatccatca
     3841 ggcgcgcttt cgcaagtatc tggaaaaccg cggcctggct ccggccggcg gcggcggccg
     3901 ggtctggtgc ttcatcggcg acggcgaggc cgacgagccc gaggtgctcg gcacgatcgg
     3961 catcgccgcg cgcgagcggc tcgacaatct ggtgctggtc gtcaactgca acctgcagcg
     4021 cctcgacggc ccggtgcgcg gcaacggcaa gatcatccag gagctcgaac gcagcttccg
     4081 cggcgccggc tggaacgtgc tgaaggtgat ctggggcagc ggctgggatc cgctgttcgc
     4141 gcgcgatcgc gacggcagcc tgatccggcg catggagtcg gccgtcgacg gcgactacca
     4201 gcgctacacg gtgctgcccg gcgaggcggt gcgcgagcac tgggtgctcg acaacccggc
     4261 gctcgccgag ctgatggcct cgctgtcgga cgaggagatc cgctcgatca agcgcggcgg
     4321 tcaggacccg ctgaagatct gggccgccta cgcgcaggcg atgcagccga acggccgccc
     4381 gaccgtgatc ctcgccaaga ccgtcaaggg cgacggcatg ggtccgggcg gcgagggccg
     4441 caacaccgcg caccagaaga agcagctgtc ggccgacgag cggcgcgact gcgcgcggcg
     4501 ctggggcatc ccgctcgccg atgaggacat cgtgcgcgcc gccttctacc gcccggccgc
     4561 cgacagcccg gagctgcgct acctgcacgc gcggcgcgag cgcctcggcg gctacctgcc
     4621 ggcgcggcgc gtcgactgcc cgccgctgcc ggcaccggcc gcggcgacgc tcgccgagag
     4681 cttcgaggga agcggcgaac gcgcgctgtc gacgacgacc gccgccgtgc gcctgctcgg
     4741 gcggctgctg cgcgacccgg cgctcgggcg gctgatcgtg ccgatcgtgc ccgacgaggc
     4801 gcgcaccttc ggcatggaag ggctgttccg cagtgccggc atctactcgc ccgagggcca
     4861 gcgctacgtg ccggtcgacg gcgacacgct gctgccgtat cgcgaggcgc gcgacgggca
     4921 gatcctgcag gaaggcatct gcgaggccgg cgcgatggcc tccttcctcg ccgccggcac
     4981 cgcctacgcc gagcacggcg tgccgagcat cccgttctac ttcttctatt cgatgttcgg
     5041 cagccagcgc gtcggcgatc tggtctgggc ctgcggcgac atgctggcgc gcggcttcct
     5101 gcttggcggc acggccgggc gcacgacgct caacggcgag ggcctgcagc accaggacgg
     5161 ccactcgccg ctgctcgccg cgaccgtgcc gaacctgaag gtctacgacc cggcctgggg
     5221 ctgggagctc gcgaccatcg tcgaggacgg catcgagcgc atgtacggac gcggcgaggc
     5281 ggtcttctac tacctgaccg tctacaacga ggcctacccg atgccggcgc gcccggccga
     5341 tccggcggcg cgcgaaggcg tgctgcgcgg cctgtaccgg ctgcgcgaac cggcgctgcc
     5401 gggggccgga cccgtgcgcg tcaacctgtt cggcagcggc agcctgctgc cgcaggcgct
     5461 ggcggcggcg gaactgctcg aagcgcgcgg gtttgcgacc gcggtctgga gcgcgaccag
     5521 ctacgtcgag ctgc
//
LOCUS       DAOQJH010000069         5491 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_8570_length_5491_cov_3.749080, whole genome shotgun sequence.
ACCESSION   DAOQJH010000069 DAOQJH010000000
VERSION     DAOQJH010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 5491)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5491)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5491
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8570_length_5491_cov_3.749080"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(242..1012)
                     /locus_tag="PLQ18_02685"
     CDS             complement(242..1012)
                     /locus_tag="PLQ18_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005408509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HNE00585.1"
                     /translation="MSTPPSPRSPRPRKVAMYEDVAERVRQKIYDHELAPGDWIDEPA
                     LAAEFGISRTPLRECLKLLAAEGLVQIDPGRGASVTKLTLEDLNELFPVMAMLEGRCA
                     FECARKLDAERLRTLETLHEALETAAAAGDVAAYYASNYVFHRTVQDFAGNPWLTRVT
                     NDLRRILKMHRGRQLLLPGRLQESLSEHRALIECFRGHDAGRAERVMNQHLLHQRVAL
                     VRYIESGGSLNGPARLPDTPGAASAPNPPYPNPQDAIA"
     gene            complement(1314..3023)
                     /locus_tag="PLQ18_02690"
     CDS             complement(1314..3023)
                     /locus_tag="PLQ18_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome D1 domain-containing protein"
                     /protein_id="HNE00586.1"
                     /translation="MLAVTSIALGLGLAPGSTHAEDASHPGVGQGEVRYQGAPSGIDA
                     AQAKDQNAGAPQMTAEEFAQAKKIFFERCAGCHGVLRKGATGKPLTPDVTRARGTDYL
                     KVFINYGSPAGMPNWGSSGELTEQQVDMMARYIQIEPPLPPEFGMKEMKDTWKVIVPP
                     AQRPTKKMNAFNIQNIFSVTLRDSGEVALVDGDTKQIINIVKTGYAVHISRLSASGRY
                     LYVIGRDARINLIDLWMEKPDNVAEIKIGLEARSVDTSKFKGYEDRFAIAGSYWPPQF
                     VIMNGDTLEPKKIVSTRGMTVDTQDYHPEPRVAAIVASHEHPEFIVNVKETGKILLVN
                     YADIDNLQVTEIGAARFLHDGGWDATHRYFLTAANKSNKVAVVDSRERKLAALVDVGA
                     IPHPGRGANFVDPQFGPVWATSHLGDDSISLIGTDPTRHKAQAWKVVRTLKGQGGGSL
                     FIKTHPQSSHLYVDTALNPDPKISQSTLVYDLKDLARAPVVLPIADWAELGEGPKRVV
                     QPEYNKDGNEVWFSVWNGKDQKSALVVVDDRTLKLKTVIKDAKLITPTGKFNVYNTQH
                     DVY"
     gene            complement(3223..>5491)
                     /locus_tag="PLQ18_02695"
     CDS             complement(3223..>5491)
                     /locus_tag="PLQ18_02695"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNE00587.1"
                     /translation="AALGLDVHARRISRPALEQSRRSGSVAVTPGLRLVQETGEQTGV
                     VFYQAVPPGRPGSEPNGFAGGVLRLGDTLAAAIAPLPRSALIVRLTDETQGAPLPLQV
                     DPDWPAHLSAGQHWSQPLVVGDRQWRLEIAPRQALPTGQHGPVAHAVQAGALLFNGVL
                     AAFLLVTSGRTTLIENIIATRTQRLQREIEDRRRAEAGLRQAAIVYEYTREGILVLDA
                     NGRVQAVNPAFERISGFTLAQWQGRRPQKLFVPRQRRRLLADIRRTLASGGHWQGETW
                     NLDHEGSPYPSWLTVSAVPGENSQPGGYIAVFSDISTIKQSQAELEHLAHHDPLTGLP
                     NRLLLGARLSHAILRARREGERMALMFLDLDRFKIVNDSLGHRIGDRLLVAAAARLAE
                     GLRASDTLARLGGDEFVLLVDRIADPEVVGRLATRLVEACNAPFTIDEHELYLGASVG
                     IALYPDDGLDAETLLRNADAAMYEAKAQGRNTYRYYLPSLTDSAEARVRIEGELRRAL
                     AHGELRLHYQPQVELADARVIGCEALVRWQHPTRGLVPPNDFLPLAEDTGLILPIGAW
                     VLDEACRQARAWLDAGHELRVAVNIAVPQVMRQDLPALVSEILARHRLPARYLELELT
                     ESLLLRDPERGAHVMSQLAELGVGLAIDDFGTGYSSLAYLKRLPIDRLKIDRLFVREL
                     PEDSDDCAIVRATLDMASHLRLSVVAEGVETAAQAVFLLAHGCGHAQGWRYGKAVPPA
                     EFPLVPVLVVPPDTP"
BASE COUNT          739 a         1833 c         2038 g          881 t
ORIGIN      
        1 ggtgcggggc acctcctcgg tcgggcggtt cggggccgga gcgcggccgg cagggaccgg
       61 gcgcacgcag cctgcgcgcg ccggtcggac ggcgttccgg gatcggtgaa gcgggggaat
      121 cagccgcggg gcggcggtgt ggcgggaggt cgcgacatga cggggctcct cggtggtctg
      181 ccgcccggtc ctgccggaga ctccggcggg cgctctgtcg gcgcgggcgg cgggcagcgc
      241 atcaggcgat ggcgtcctgc gggttcggat acggcggatt cggcgcactg gcggcgccgg
      301 gcgtgtccgg cagccgcgcc gggccgttga ggctgccgcc ggattcgatg tagcggacca
      361 gcgcgacgcg ctggtgcagc aggtgctggt tcatcacgcg ctcggcgcgc ccggcgtcgt
      421 ggccccggaa gcactcgatc agcgcgcggt gttcggacag cgattcctgc agccgccccg
      481 gcagcagcag ctggcggccg cggtgcatct tcaggatgcg gcgcaggtcg ttggtcacgc
      541 gcgtcagcca cgggttgccg gcgaagtcct gcaccgtgcg gtgaaagacg tagttgctcg
      601 cgtagtacgc ggccacgtcg ccggcggcgg cggcggtctc gagcgcctcg tgcagggttt
      661 cgagcgtccg gaggcgctcg gcatcgagct tgcgcgcgca ttcgaaggcg cagcggcctt
      721 cgagcatcgc catgaccggg aacagctcgt tgaggtcttc gagcgtgagc ttggtgacgc
      781 tggcaccgcg ccccgggtcg atctgcacca gcccctcggc ggcgagcagc ttcaggcact
      841 cgcgcagcgg cgtgcggctg atgccgaact cggcggcgag cgccggttcg tcgatccagt
      901 cgccgggggc gagttcgtgg tcgtagatct tctggcgcac gcgctcggcg acatcctcgt
      961 acatcgcgac cttgcgcggg cgcgggctgc ggggggaagg cggggtgctc atgatccggg
     1021 gactccgcgg ttcggtgatg atggtgtgat tacatcataa ttatgaaaaa atacaagccg
     1081 cggcttagat tttttcaggg catgcgcgcg caggcgcgcc ggggaggatc ggcagccgga
     1141 gccgggaacg atcggcgcgc cggggcggct gcggagccgg aagaaccgca gccggccggg
     1201 acggtcgccg cgaaaaaaag cgggggcgga ccgcaggacc ggtccgcccc cgccgtgaga
     1261 ctggcgcgac gcttcccccc ccccgatccg ccggggagcg gctcgttgct ggctcagtac
     1321 acgtcgtgct gcgtgttgta gacgttgaac ttgccggtcg gcgtgatcag cttcgcgtcc
     1381 ttgatgacgg tcttgagctt cagcgtccgg tcgtcgacga cgacgagcgc cgacttctgg
     1441 tccttgccgt tccagaccga gaaccagacc tcgttgccgt ccttgttgta ctcgggctgc
     1501 acgacgcgct tcggaccctc gccgagctcg gcccagtcgg cgatcggcag caccaccggc
     1561 gctctggcga gatccttcag gtcgtagacc agcgtcgact ggctgatctt cggatccggg
     1621 ttcagcgcgg tgtcgacgta caggtggctc gactgcgggt gggtcttgat gaacagcgaa
     1681 ccgcccccct gacccttcag cgtgcgcacg accttccagg cctgggcctt gtgtctggtt
     1741 gggtcggtgc cgatcagcga gatgctgtca tcgccgaggt ggctggtggc ccagaccgga
     1801 ccgaactgcg gatcgacgaa gttcgcgccg cgccccgggt gcgggatcgc gccgacatcg
     1861 acgagggcgg cgagcttgcg ctccctggag tcgaccacgg cgaccttgtt ggacttgttc
     1921 gcggcggtca ggaagtagcg gtgcgtcgcg tcccagccac cgtcgtgcag gaagcgcgcg
     1981 gcgccgatct cggtgacctg caggttgtcg atgtcggcgt agttgaccag caggatcttg
     2041 ccggtctcct tgacgttgac gatgaactcc gggtgctcgt gcgaggcgac gatcgcggcg
     2101 acgcgcggct cggggtggta gtcctgcgtg tcgacggtca tgccgcgcgt cgagacgatc
     2161 ttcttcggct ccagcgtgtc gccgttcatg atcacgaact gcggcggcca gtaggagccg
     2221 gcgatcgcga accggtcctc gtagcccttg aacttcgagg tgtcgaccga gcgcgcttcg
     2281 aggccgatct tgatctcggc gacgttgtcg ggcttctcca tccacaggtc gatcaggttg
     2341 atgcgcgcat cgcggccgat cacgtacagg tagcggcccg aggccgacag ccgcgagatg
     2401 tgcacggcgt agccggtctt gacgatgttg atgatctgct tggtgtcgcc gtcgaccagc
     2461 gccacctcgc cggagtcgcg cagcgtcacc gagaagatgt tctggatgtt gaaggcattc
     2521 atcttcttcg tcgggcgctg cgcgggcggc acgatgacct tccaggtgtc cttcatttcc
     2581 ttcatgccga attcgggcgg cagcggcggc tcgatctgga tgtagcgcgc catcatgtcg
     2641 acctgctgct cggtcagctc gcccgagctg ccccagttcg gcatgccggc cggcgagccg
     2701 tagttgatga acaccttcag gtaatcggtg ccgcgtgcgc gcgtgacatc gggcgtcagc
     2761 ggcttgccgg tcgcgccctt gcgcagcacg ccgtggcagc cggcgcagcg ctcgaagaag
     2821 atcttcttgg cctgcgcgaa ctcctcggcc gtcatctgcg gcgcgccggc gttctggtcc
     2881 ttcgcctgcg cggcgtcgat gccgctcggc gcgccctgat agcggacctc gccctggccg
     2941 acgcccgggt ggctggcgtc ctcggcgtgc gtgctgccgg gcgcgaggcc gaggccgagg
     3001 gcgatcgacg tgacggccag cagcggggcc gtgctgcggt gtttcggctg attcatgttc
     3061 tggtgctccc ccgtgcgact tgcggatggc tgcggaactc gtgcgctgcg gtcgagcgga
     3121 tgcgaccgtt gcgcctgctt gccgcggcta tctgacgccc ggggggcggg gccgaccctt
     3181 gacgctcatc aagccgcgcc cgggcacaca ggagactgat ccctaagggg tatccggggg
     3241 taccaccagc accggcacca gcgggaactc cgcgggcggc acggccttgc cgtagcgcca
     3301 gccctgggcg tgaccgcagc cgtgcgcgag caggaatacc gcctgcgcgg cggtctcgac
     3361 gccttcggcg accaccgaca ggcgcaggtg actggccatg tccagcgtcg cgcgcacgat
     3421 cgcgcagtcg tcgctgtcct cgggcagctc gcgcacgaac agccggtcga tcttcagccg
     3481 gtcgatcggc aggcgcttga ggtaggcgag cgaggaatag ccggtgccga agtcatcgat
     3541 cgccagcccg acgccgagct cggcgagctg cgacatgacg tgtgcgccgc gctccggatc
     3601 gcgcagcagc agcgattcgg tcagctccag ctccagatag cgcgccggca ggcggtggcg
     3661 cgcgaggatt tccgagacca gcgccggcag gtcctgacgc atgacctgcg gcacggcgat
     3721 gttgaccgcc acgcgcagtt cgtggccggc atccagccag gcgcgcgcct ggcggcaggc
     3781 ctcgtcgagc acccaggcgc cgatcggcag gatcaggccg gtgtcctcgg cgagcggcag
     3841 gaagtcgttc ggcggcacca gcccgcgggt cggatgctgc cagcgcacca gcgcctcgca
     3901 gccgatcacg cgggcatcgg cgagttcgac ctgcggctgg tagtgcaggc gcagttcgcc
     3961 gtgggcgagc gcgcggcgca gttccccctc gatgcgcacg cgcgcctcgg cactgtcggt
     4021 caggctcggc aggtagtagc ggtaggtgtt gcggccctgg gccttggcct cgtacatcgc
     4081 ggcgtcggcg ttgcgcagca gcgtttcggc gtcgagcccg tcatcgggat acagcgcgat
     4141 gccgacgctg gccccgaggt agagctcgtg ctcgtcgatc gtgaacgggg cgttgcaggc
     4201 ctcgaccagg cgcgtcgcca gccggccgac cacctccgga tcggcgatgc gatcgaccag
     4261 cagcacgaac tcgtcgccgc cgaggcgcgc cagcgtgtcg ctggcgcgca ggccctcggc
     4321 gagccgtgcc gccgccgcca ccagcagccg gtcgccgatg cggtggccga ggctgtcgtt
     4381 gacgatcttg aagcggtcga gatcgaggaa catcagcgcc atgcgctcgc cctcgcgccg
     4441 ggcgcgcagg atcgcgtggc tcaggcgcgc cccgagcagc agccggttcg gcagcccggt
     4501 cagcgggtcg tggtgggcga ggtgttcgag ttccgcctgc gactgcttga tcgtcgagat
     4561 gtccgagaac accgcgatgt agccgccggg ctggctgttc tcgcccggta cggcgctgac
     4621 cgtcagccag gacggatacg gcgagccttc gtgatcgagg ttccaggtct cgccctgcca
     4681 gtggccgccg gaggcgagcg tgcggcggat gtcggcgagc agccgccggc gctggcgcgg
     4741 cacgaacagc ttctgcgggc gccggccctg ccactgcgcg agcgtgaaac cgctgatgcg
     4801 ctcgaaggcc gggttcaccg cctgcacgcg gccgttggca tcgagcacca ggatgccctc
     4861 gcgcgtgtac tcgtagacga tcgccgcctg ccgcagcccg gcctcggcgc ggcggcggtc
     4921 ctcgatctcg cgctgcaggc gctgcgtgcg cgtggcgatg atgttctcga tcagcgtcgt
     4981 gcgccccgag gtcacgagca ggaaggccgc gagcacgccg ttgaacagca gcgcaccggc
     5041 ctgcacggcg tgcgcgaccg gtccgtgctg gccggtcggc aacgcctggc gcggcgcgat
     5101 ctccagccgc cactggcgat cgccgaccac cagcggctgg ctccagtgct ggccggcgga
     5161 caggtgcgcc ggccagtcgg ggtcgacctg cagcggcagc ggcgcgccct gggtctcgtc
     5221 ggtcaggcgc acgatcaggg cgctgcgcgg cagcggcgcg atcgccgcgg ccagcgtgtc
     5281 gccgagcctg agcacgccgc cggcgaagcc gttcggttcg gaaccggggc ggcccggcgg
     5341 caccgcctga tagaacacga cgccggtctg ctcgccggtc tcctgcacca ggcgcaggcc
     5401 gggcgtgacc gcgacgctgc cgctgcggcg cgactgttcc agcgccgggc gcgagatgcg
     5461 gcgggcgtgg acatcgagac cgagcgccgc c
//
LOCUS       DAOQJH010000070         5480 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_8597_length_5480_cov_3.871336, whole genome shotgun sequence.
ACCESSION   DAOQJH010000070 DAOQJH010000000
VERSION     DAOQJH010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 5480)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5480)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5480
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8597_length_5480_cov_3.871336"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..444)
                     /locus_tag="PLQ18_02700"
     CDS             complement(<1..444)
                     /locus_tag="PLQ18_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNE00588.1"
                     /translation="MDFDHGISAIDTGFERPLFDASHLIIDHGRAAFVDAGVNASVPM
                     LIGVLEHKGLTPAAVDWLLLTHVHLDHAGGAGELLRRLPNAKLVVHPRGVRHMVDPTA
                     LMAGASAVYGEDVVRRTYGTLTPVPPGRIVEATEGLELVLGSRRLT"
     gene            790..1377
                     /locus_tag="PLQ18_02705"
     CDS             790..1377
                     /locus_tag="PLQ18_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013234823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE00589.1"
                     /translation="MVTIQETPFAGLEADGRLLSPVHKGPTKKPGRFGFRGVIALEFA
                     EKMSDEARPPSIKADQVMAVGDAGASSLPFVAAMTLSAKHLPLLVEVLGERLVPEGKY
                     FLFATDVDFSERWQLSYNGIPFWVLPLDEATVYNEVLELVGVEKSDLKKLDTAGKLDA
                     VADAAAGFSDSFPAISYEDLVARLGSVRVNEYRPV"
     gene            complement(1402..1578)
                     /locus_tag="PLQ18_02710"
     CDS             complement(1402..1578)
                     /locus_tag="PLQ18_02710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE00590.1"
                     /translation="MDTGRRRYPFIWFERGGWWFKIFGEVFGPFANATRAHDSFVLAR
                     RLDSGWYGEGRDAR"
     gene            complement(1669..2667)
                     /locus_tag="PLQ18_02715"
     CDS             complement(1669..2667)
                     /locus_tag="PLQ18_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018181325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HNE00591.1"
                     /translation="MPAVPATMNAVGIRTPGGPEVLQPCTRPVPQPRAGEVLIEVAAA
                     GVNRPDCLQRAGAYPPPPGASDLPGLEVAGCVVAVGAGVDWPAAGEHVCALCNGGGYA
                     GYVAVPAGQCLPVPAGWSDIEAAALPETFFTVWTNLFERGRLAAGESVLVHGGAGGIG
                     STAIRLATAFGATVYASAGSAEKCAACLAAGAARAIDHTREDFVTVVRSETGGRGVDV
                     ILDIVGAGYLARHLDLLAPEGRLVLIAGLQGYKAELNLWPLLAKRLSITGSTLRARSD
                     ADKAAIATALHERVWPLLDAGRLRPPPPATLALAQAAEAHRRMEANSLIGKLVLLP"
     gene            2811..4004
                     /locus_tag="PLQ18_02720"
     CDS             2811..4004
                     /locus_tag="PLQ18_02720"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE00592.1"
                     /translation="MAELRRILFVDNENTVLRELETGLAAMRGQWQMEFISDARAALQ
                     RLDEERFDALIADPCLSGKDGASLLAEAAQRHPPLVRIVLAGPSERSAALDALAVAHQ
                     VLAKPCDPAELRATIGRALALRKFLGGDTPLKALVSRLDSLPSMPALYRRIVEELQSA
                     HASFESVAAIIGQDPPMAAKILQLVNSAYFTRAQHVSDLSQALRMLGLDTIKALVLSL
                     QVFSQFDAETLAGLSVERIWRHSVRTGALARAIARAERAPREVENDAFTAALLHDVGK
                     LILAANLPNIYAKIGPLCDAEALEPCAAERRLFGAAHAETGAYLLGLWGLPESIVEAV
                     AYHHHPRDREPAGICALTWVHAANALAQLPDAPVGVLDAEYLGALDLLERLPAWAALG
                     EAMPD"
     gene            4147..4548
                     /locus_tag="PLQ18_02725"
     CDS             4147..4548
                     /locus_tag="PLQ18_02725"
                     /inference="COORDINATES: protein motif:HMM:NF024503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HNE00593.1"
                     /translation="MTSTLRAARLLAAACLIAGLLTGCAGSGPSGPDSYEAAQTQFRS
                     RVQQAWVAPKSFAKTPRALSTTVRISSDATGAVKEVKMIRRSGRRDFDASVVKAVLDA
                     SPLPVAQDKALATRLQSLELEIGTDAQGRMR"
     gene            4663..>5480
                     /locus_tag="PLQ18_02730"
     CDS             4663..>5480
                     /locus_tag="PLQ18_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011813271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HNE00594.1"
                     /translation="MTAGIPRVADWHAQTPDSVRTTLATPDTGLTAADAAGRLEHHGP
                     NRLPPPKTVGPWKRFLRQFDNVLIYVLLASAATTAALGHPVDTAVILGVVLINAIIGF
                     VQEGRAESALAAIRDLLSLRACVLRDGERREIAAEELVPGDRVLLASGDKVPADLRLV
                     SVRSLRVQEAALTGESEPVEKDTAAVAADAALGDRRCMAYSGTLVTYGQAQGLVVATG
                     ADTEIGRISALLAQVEPLTTPLLEQINRFGRWLTGVILILAAATFLFGWLVRGLP"
BASE COUNT          761 a         1993 c         1943 g          783 t
ORIGIN      
        1 cgtcagccgg cggctgccga gcacgagttc gaggccctcg gtggcctcga cgatgcgccc
       61 gggcggcacc ggcgtcagcg tgccgtaggt gcgccgcacc acgtcctcgc cgtagacggc
      121 cgaggcgccg gccatcagcg ccgtcggatc gaccatgtgg cgcacgccgc gtggatgcac
      181 gaccagcttc gcgttcggca gccggcgcag cagctcgccg gcgccgccgg cgtggtcgag
      241 gtggacgtgc gtcagcagca gccagtcgac cgcggcgggc gtgaggccct tgtgttcgag
      301 cacgccgatc agcatcggca ccgaggcgtt gacgccggca tcgacgaagg cggcccggcc
      361 gtggtcgatg atcaggtggc tcgcgtcgaa cagcgggcgt tcgaagccgg tgtcgatcgc
      421 gctgatgccg tggtcgaaat ccagaaagct cagcgtcccg tggtcgagac gctcgatggc
      481 gacggcggtc ggtggattca cgcggggccc cgggcggtcg gcagatctgc aggagtgccg
      541 agtgtaaccg catcctccgc ggcccggacc gcccgccggc ggctgtcgca aacccgacaa
      601 gtcgcggagc ttgtcggcaa gccccccggg cgatgccggg tcgctcagga gggcggagtt
      661 ttcatctgat tgatgaagat ggataaaacc ggtttttcac agaatggtat ggaccttgct
      721 caaccccgat ctgcggcgcg tgcgccccag ttttcgtacc tcgtccatac catttccggg
      781 agaccctcca tggtcacgat tcaggaaacc cccttcgccg gcctggaggc cgacgggcga
      841 ctgctgtcgc cggtgcacaa gggcccgacc aagaagccgg ggcgcttcgg cttccgcggc
      901 gtgatcgcgc tcgaattcgc cgagaagatg tccgacgaag cccgcccgcc gtccatcaag
      961 gccgatcagg tcatggcggt cggtgatgcc ggggcgagca gcctgccgtt cgtcgcggcg
     1021 atgacgctgt cggcgaagca tctgccgctg ctggtcgagg tgctcggcga gcggctggtg
     1081 cccgagggca agtacttcct gttcgcgacc gacgtcgatt tctccgagcg ctggcagctc
     1141 agctacaacg gcatcccgtt ctgggtgctg ccgctcgacg aggccaccgt ctacaacgaa
     1201 gtgctcgaac tcgtcggcgt cgagaagagc gacctgaaga agctcgacac cgccggcaag
     1261 ctcgatgcgg tcgccgacgc cgccgccggc ttctcggaca gcttcccggc gatcagctac
     1321 gaggacctcg tcgcccgcct cggcagcgtg cgcgtcaacg agtaccgccc ggtctgatcc
     1381 ggcgcacggg acgcgcagcc ttcagcgcgc gtcccggcct tcgccgtacc agccgctgtc
     1441 gagccggcgt gccagcacga agctgtcgtg cgcgcgcgtc gcgttcgcga acggcccgaa
     1501 cacttccccg aagatcttga accaccagcc cccccgttcg aaccagatga aggggtagcg
     1561 cctgcgtccc gtgtccactg ccgcctccgc cgcctgtgcg gcccgctgtg catggcaagt
     1621 accatgtctg cggataacgg ccgggccggc ggctacttga gcccggcttc agggcagcag
     1681 caccagcttg ccgatcaggc tgttggcctc catgcgccgg tgcgcctcgg cggcctgcgc
     1741 gagcgcgagc gtcgccggtg gcggcgggcg cagccggccg gcgtcgagca gcggccagac
     1801 ccgctcgtgc agcgccgtgg cgatcgcggc cttgtcggcg tcgctgcgcg cgcgcagcgt
     1861 cgagccggtg atgctcaggc gcttcgcgag cagcggccag aggttcagct cggccttgta
     1921 gccctgcagg ccggcgatca gcaccagccg gccctcgggc gccagcaggt cgagatgacg
     1981 cgccagatag ccggcaccga cgatgtcgag gatcacgtcg acaccgcgcc cgccggtttc
     2041 gctgcgcacg acggtgacga agtcctcgcg cgtgtgatcg atcgcgcgcg cagcaccggc
     2101 ggcgaggcag gccgcgcatt tttccgcgct gccggcgctc gcgtagaccg tggcgccgaa
     2161 ggccgtcgcc agccggatcg cggtcgagcc gatgccgccg gccccgccgt gcacgagcac
     2221 gctttcgccg gcggcgagcc ggccgcgctc gaacagattg gtccagaccg tgaagaaggt
     2281 ctcgggcagc gccgcggcct cgatgtcgct ccagcccgcc ggcaccggca ggcactggcc
     2341 ggccggcacc gcgacgtagc cggcgtagcc gccgccgttg cagagcgcgc agacgtgctc
     2401 gccggccgcc ggccagtcga cgccggcgcc gacggcgacg acgcagccgg cgacttccag
     2461 tcccggcagg tccgaggcgc cgggcggcgg cgggtaggcg ccggcgcgct gcaggcagtc
     2521 cgggcggttg acgccggccg cggcgacctc gatcagcacc tcgccggcgc gcggctgcgg
     2581 caccggtcgc gtgcacggct gcagcacctc ggggccgccg ggcgtgcgga tcccgacggc
     2641 gttcatcgtg gccggcacgg caggcatggg cggcatccgg gtgagggtgg gcggccgcga
     2701 gtattgaggg tcgccggggg ctgcgacaag cgcggtgggc gtgacacttt gcgttacgat
     2761 gcccgccgcc gcggcatccg gcccggctgg cagcggagcg gaatctgacg atggcggagt
     2821 tgcggcgcat cctgttcgtc gacaacgaga acacggtcct gcgtgagctg gagaccgggc
     2881 tcgcggccat gcgcgggcag tggcagatgg aattcatcag cgatgcgcgc gccgcgctgc
     2941 agcgcctcga cgaggagcgc ttcgatgcgc tgatcgccga tccctgcctg agcggcaagg
     3001 acggcgcaag cctgcttgcc gaggccgcgc agcgccatcc gccgctggtg cgcatcgtgc
     3061 tcgccgggcc gtcggagcgc agcgccgccc tcgatgcact cgccgtcgcg catcaggtgc
     3121 tcgcgaagcc ctgcgatccg gccgagctgc gcgcgaccat cggccgggcg ctggcgctgc
     3181 gcaagttcct cggtggcgac acgccgctga aggcgctggt gtcgcggctc gactcgctgc
     3241 cgagcatgcc ggcgctgtac cggcgcatcg tcgaggagct gcagtcggcg catgcctcgt
     3301 tcgagtcggt cgcggcgatc atcggccagg acccgccgat ggccgcgaag atcctgcagc
     3361 tcgtgaactc ggcctacttc acgcgcgccc agcacgtcag cgacctgtcg caggcactgc
     3421 gcatgctcgg cctcgacacg atcaaggcgc tggtgctgtc gctgcaggtg ttctcgcagt
     3481 tcgacgccga gacgctggcc gggctgtcgg tcgagcgcat ctggcgccac agcgtgcgca
     3541 ccggcgcgct ggcccgcgcc atcgcccgcg ccgagcgcgc gccgcgcgag gtcgagaacg
     3601 acgccttcac cgccgcgctg ctgcacgatg tcggcaagct gatcctcgcc gccaacctgc
     3661 cgaacatcta cgcgaagatc gggccgctct gcgatgccga ggccctggaa ccctgcgccg
     3721 ccgaacggcg gctgttcggt gccgcgcacg ccgagaccgg cgcctacctg ctcgggctgt
     3781 gggggctgcc ggagagcatc gtcgaggcgg tcgcctacca ccatcacccg cgcgaccgcg
     3841 aaccggccgg catctgcgcg ctgacctggg tgcatgccgc caacgcgctg gcgcagctgc
     3901 cggatgcccc ggtcggcgtg ctcgatgccg agtacctcgg cgcgctcgac ctgctcgaac
     3961 gcctgccggc ctgggctgcg ctcggcgagg ccatgcccga ctgatcgccg gggcggggcg
     4021 gcggcggcct tcgcttgtcg cccgtcgatg gctgccgcta ggctgtgccg ctttcctgcc
     4081 cggcgccgtg ccccggtcgt gcggccggcg ccctcgatgg atcgtccacc cggaacctgc
     4141 aagcccatga ccagcaccct gcgcgcggcc cgcctgctcg ccgccgcctg tctcatcgcc
     4201 ggactcctca ccggctgcgc cggcagcggc ccgagcggcc ccgacagcta cgaagccgcc
     4261 cagacccagt tccgcagccg cgtgcagcag gcctgggtcg caccgaagtc gttcgcgaag
     4321 acgccgcggg cactgtcgac gaccgtgcgc atcagcagcg atgccaccgg cgcggtcaag
     4381 gaagtgaaga tgatccggcg cagcggccgg cgcgacttcg acgcctcggt ggtcaaggcc
     4441 gtgctcgatg cctcgccgct gccggtggcg caggacaagg cgctggctac gcgcctgcag
     4501 agcctggagc tggagatcgg caccgacgcg cagggccgga tgcgctgatc ccagcccatc
     4561 cctgccggaa attccggcgc cggcgtatcc ggcagcggtg aaccgggcga tgatcgtcca
     4621 ctcttacagg tgggatttct ccacccacct gcagggggca ccatgactgc cggcatcccg
     4681 cgcgtcgccg actggcacgc gcaaacgccc gattccgtcc gcacgaccct cgccacgccc
     4741 gacaccgggc tcaccgccgc cgacgcggcc gggcggctcg aacatcacgg cccgaaccgc
     4801 ctgccgccgc cgaagacggt cgggccgtgg aagcgcttcc tgcgccagtt cgacaacgtg
     4861 ctgatctacg tgctgctcgc ctcggcggcg accacggccg cgctcggaca tccggtcgac
     4921 accgccgtga tcctcggcgt cgtgctgatc aacgccatca tcggtttcgt gcaggaaggc
     4981 cgcgccgaat cggcgctggc ggcgatccgc gacctgctgt ccctgcgcgc ctgcgtgctg
     5041 cgtgacggcg agcggcgcga gatcgccgcc gaggaactcg tgcccggcga ccgggtgctg
     5101 ctcgccagcg gtgacaaggt gccggccgac ctgcgcctgg tgtcggtccg gagcctgcgc
     5161 gtgcaggagg cggcgctgac cggcgagtcc gagccggtcg agaaggacac ggccgccgtc
     5221 gcggccgacg ccgcactcgg cgaccgccgc tgcatggcct attccggcac gctggtcacc
     5281 tacggtcagg cgcaggggct ggtggtcgcc accggtgccg ataccgagat cggccgcatc
     5341 agcgcactgc tcgcccaggt cgagccgctg accacgccgc tgctcgaaca gatcaaccgc
     5401 ttcggccgct ggctgaccgg cgtgatcctg atcctcgccg ccgcgacctt cctgttcggc
     5461 tggctggtgc gcggcctgcc
//
LOCUS       DAOQJH010000071         5442 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_8685_length_5442_cov_4.092445, whole genome shotgun sequence.
ACCESSION   DAOQJH010000071 DAOQJH010000000
VERSION     DAOQJH010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 5442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5442
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8685_length_5442_cov_4.092445"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(238..2184)
                     /gene="recD"
                     /locus_tag="PLQ18_02735"
     CDS             complement(238..2184)
                     /gene="recD"
                     /locus_tag="PLQ18_02735"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003286786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HNE00595.1"
                     /translation="MTESAAPDTDPRARVDDELDRLLAAGAIGPLDREFARLLQRLVP
                     KPAPRAGLLAAALASRQIGLGHVCLPLTGFEGFRRRLLAEDEQGPAIWAPAAAEWIER
                     LRRWPAVGSPGEFRPLVLDADGRLYLYRYWRYETGLAARLVELAQAPVRVDEPRLRQG
                     LDRLFPQAAADAAGPDLQRLACANAALSRLALICGGPGTGKTTTLAKLLALLLMHDGD
                     SLRIRLAAPTGKAAARMLEALRGARDRLAGQLEPALLAALPTDASTLHRLLGVRPDST
                     ARFRHDAGHPLALDVLVIDEVSMVDLPLLAKTVAALPAHARLILLGDQDQLASVEAGA
                     VLGELGAQAGAPSPQRAAQLAAISGCTIEADPEPLLPPLRDCVALLRRSYRFSGASGI
                     GALARAVNAGDAGAALAQLEAGAEDLQWSQATAIEAVIERARSGYRSYLQLLGTAAAA
                     QDPQALHAAFARFRVLGGLRSGALGVETLNARLEREARRAAGRPDTALWYPGRPVMVL
                     RNDYGLNLYNGDIGIATVDAEGTLAVQFPRPEGGFRALPVARLPEHEPVFAMTVHKSQ
                     GSEYDEVLLVAPPADSPLATRELLYTGLTRARAQLGIVASAEAVQLACSRRVARDSGL
                     AGRLAEAAAGTLVQPPGVSPRTPG"
     gene            complement(2272..2817)
                     /locus_tag="PLQ18_02740"
     CDS             complement(2272..2817)
                     /locus_tag="PLQ18_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008176836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HNE00596.1"
                     /translation="MSIARLLSAAALAALLSACMPLSPLPAEAPADATAVSGRLPKAD
                     LILVKKSDRRLTLLRNGREIKSYKVSLGRNPGGQKVAAGDGRTPEGDYLIDWRKPDSK
                     FYKALHISYPRVSDRRTAEEAGRNPGGAIMIHGLPNDRPRAHHYLPGIDWTEGCIAVT
                     NREMDEIWHSVRDGTPIRILP"
     gene            2965..4164
                     /gene="dapC"
                     /locus_tag="PLQ18_02745"
     CDS             2965..4164
                     /gene="dapC"
                     /locus_tag="PLQ18_02745"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017639059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009016 - succinyldiaminopimelate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019877 - diaminopimelate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyldiaminopimelate transaminase"
                     /protein_id="HNE00597.1"
                     /translation="MNPDLDRLQPYPFEKLARLKSGLHAPHGLAHIPLYIGEPQHPAP
                     QLALDALVANLGALSNYPLTRGTPALRAAIADWAVRRYRLTGLDADRQVLPVAGTREA
                     LFAFAQAVVARSDTAAGRPLVLMPNPFYQIYEGAALLAGAEPYFLNTPAGTGFLPDYD
                     AVPEEVWARCQLLYLCSPGNPTGAVTPAATLAKLIDKADEFGFVIAADECYSEIYFDE
                     AAPPVGLLEACARLGRHDYRRCVVFNSLSKRSNLPGLRSGFVAGDADVLAAFLQYRTY
                     HGCALPGSTQAASVAAWGDEAHVQANRALYRAKLDAVLDVLGPVLDVSRPEAGFFLWP
                     RVPGDDERWAQQLFAGQHVTVLPGRYLARDTALGNPGAGRVRIALVAPLADCTEAAIR
                     IRRCLGA"
     gene            4207..5025
                     /gene="dapD"
                     /locus_tag="PLQ18_02750"
     CDS             4207..5025
                     /gene="dapD"
                     /locus_tag="PLQ18_02750"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020484620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HNE00598.1"
                     /translation="MNKLQTLIEDAFERRAEYSPASHPLEVRQAVEEALALLEAGQAR
                     VAEKQGTGWHVNQWLKKAVLLSFRLNDNRVLGDGPLRAFDKVPTRYGDWDEARFREGG
                     ARVVPGAVVRGGSFVAKNVVLMPSFVNIGAYVDEGTMVDTWATVGSCAQIGRNVHLSG
                     GVGIGGVLEPLQAGPTIIEDNCFIGARSEVVEGVVVEEGSVISMGVFIGQSTKIYNRL
                     TGEVSYGRVPAGSVVVSGSLPAPDGSHSLYCAVIIKQVDERTRGKVGLNELLRD"
     gene            complement(5226..>5442)
                     /locus_tag="PLQ18_02755"
     CDS             complement(5226..>5442)
                     /locus_tag="PLQ18_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020157639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNE00599.1"
                     /translation="GAGRPMPAAVATAPEEASINLEAVERRHIEQVLTHCSGVIEGPK
                     GAAAALGLHPSTLRSRMQKLGIRTRRT"
BASE COUNT          763 a         1973 c         1925 g          781 t
ORIGIN      
        1 cgccaactgg taagttggta tgaccagaaa aagaattggt ctggcttatg atccatcatg
       61 caagccgaca tcgacctccg ccctgacgcc ggtcaggcag tcgcgatggg atccccgccg
      121 gcaaccgcgc ccggtcatgg cagcgaccgg ccgctttcgc cacacggcgc ctgccgcctc
      181 accgccgaag gcagcgcgcg ccggtacggc gcctcctccg gcgcttgcca tccggaatca
      241 gccgggcgtg cgcggcgaca cgcccggggg ctgcaccagc gtgccggccg cagcctcggc
      301 cagacgcccg gcaagccccg agtcccgcgc gacgcgccgg ctgcaggcga gctggaccgc
      361 ctctgcggat gcgacgatgc cgagctgcgc gcgcgcgcgc gtcaggcccg tgtacagcag
      421 ttcgcgcgtc gccagcggcg agtcggccgg cggcgcgacc agcagcacct cgtcgtactc
      481 cgagccctgc gacttgtgca cggtcatcgc gaacaccggc tcgtgctcgg gcaggcgcgc
      541 gaccggcagt gcgcggaagc cgccctcggg ccgcgggaac tgcacggcga gcgtgccttc
      601 ggcgtcgacg gtggcgatgc cgatatcgcc gttgtagagg ttcaggccgt agtcgttgcg
      661 cagcaccatc accggccggc ccggatacca cagcgccgtg tccggccggc cggcggcccg
      721 gcgcgcctcg cgttccagcc gcgcgttcag cgtctcgacc ccgagtgccc cgctgcgcag
      781 gccaccgagc acgcgaaagc gcgcgaaggc tgcgtgcagt gcctgcgggt cctgcgccgc
      841 cgcggcggtg ccgagcagct gcagatagct tcgataaccg ctgcgcgccc gctcgatcac
      901 cgcctcgatc gccgtcgcct gcgaccactg caggtcttcg gcgccggctt cgagctgcgc
      961 gagcgcggcc ccggcatcgc cggcattgac ggcgcgcgcc agcgccccga tgccgctggc
     1021 gccggagaag cggtagctgc gccgcagcag cgccacgcag tcgcgcagcg gcggcagcag
     1081 cggttcggga tcggcctcga tggtgcagcc gctgatcgcc gcgagctgcg cggcccgctg
     1141 cggcgacggg gcaccggcct gcgcgccgag ctcgccgagc acggccccgg cctcgaccga
     1201 ggcgagctga tcctggtcac cgagcaggat cagccgcgcg tgcgccggca gcgcggcgac
     1261 ggtcttggcg agcagcggca ggtcgaccat cgacacctcg tcgatcacca gcacgtcgag
     1321 cgcgagcgga tgtccggcgt cgtggcgaaa gcgcgccgtc gaatccggac gcacgccgag
     1381 cagccggtgc agcgtcgagg catcggtcgg cagcgccgcg agcagcgcgg gttcgagctg
     1441 cccggccagc cggtcgcggg caccgcgcag cgcttcgagc atgcgcgccg ccgccttgcc
     1501 ggtcggcgcc gccagccgga tgcgcaggct gtcgccatcg tgcatcagca gcagcgccag
     1561 cagcttcgcg agcgtcgtgg tcttgccggt gccgggtccg ccgcagatca gcgcgagccg
     1621 ggagagcgcc gcattggcgc aggccagccg ctgcaggtcc gggccggcgg cgtccgcggc
     1681 cgcctgcgga aacagccggt ccagcccctg ccggagccgt ggctcatcga cgcgcaccgg
     1741 cgcctgcgca agctccacca gccgcgccgc cagcccggtc tcgtagcgcc agtagcggta
     1801 caggtacagc cggccgtccg catcgagcac cagcggccgg aactcgccgg gactgccgac
     1861 cgccggccac cggcgcagcc gctcgatcca ctcggccgca gccggagccc agatcgccgg
     1921 gccctgttca tcctcggcga gcaggcggcg gcggaagcct tcgaagccgg tcagcggcag
     1981 gcagacatgg ccgagaccga tctggcggct ggcgagcgcg gcggcgagca ggccggcgcg
     2041 tggcgccggc ttcggaacga ggcgctggag caggcgcgca aactcgcgat cgagcggacc
     2101 gatggcgccg gcggcgagca ggcggtcgag ttcgtcgtcg acccgcgcgc gcggatcggt
     2161 atcaggggca gcggactcgg tcatcggcgg ttgcggcggc agtctaagga ctgcctcagc
     2221 ctcgaccggc caccggggac cgtcaagccg ccgccggtcc gggatgcggc ctcagggcag
     2281 gatgcggatc ggcgtgccgt cgcgcaccga gtgccagatc tcgtccatct cgcggttggt
     2341 gaccgcgatg cagccctcgg tccagtcgat gcccggcaga tagtgatgcg cgcgcggccg
     2401 gtcattcggc aggccgtgga tcatgatcgc gccaccgggg ttgcggccgg cttcctcggc
     2461 ggtgcgccgg tcgctgacgc gcggatacga gatgtgcagc gccttgtaga acttgctgtc
     2521 cggcttgcgc cagtcgatca ggtaatcgcc ttcgggcgtg cgcccgtcgc cggcagcgac
     2581 cttctggccg cccggattgc ggccgagcga aaccttgtag cttttgattt ccctgccgtt
     2641 tctcagcagc gtcaaccggc ggtcggactt cttcacgagg atgagatcgg ccttcggcaa
     2701 gcggccgctg acggccgttg catcggcggg cgcttcggcc ggcagcgggg acagcggcat
     2761 gcaggccgac agcagtgcgg ccagcgccgc agcagaaagc agacgtgcaa tcgacatgat
     2821 gtatcagtct ggataatcga caacaaagca acggattgtt gcgtgcgcgc acaataccat
     2881 ggccggccgc ggctatggtg cccgcgcccg cgcggcttat actccgcgcc ccttgccgga
     2941 ccgatccgta ccgatacttc gcgcatgaat cccgatctcg accgcctgca accctatccg
     3001 ttcgagaagc tggcccggct gaagtccggc ctgcacgcgc cgcacgggct cgcgcacatc
     3061 ccgctgtaca tcggcgagcc gcagcatccg gcgccgcagc tcgcgctcga tgcgctggtt
     3121 gcgaacctcg gtgcgctgtc gaactacccg ctgacccggg gcacgccggc gctgcgcgcc
     3181 gcgatcgctg actgggcggt gcgccgctac cggctgacgg gactcgatgc ggaccggcag
     3241 gtgctgccgg tggccggcac gcgcgaggcg ctgttcgcgt tcgcgcaggc ggtggtggcg
     3301 cgcagcgaca cggccgcggg ccggccgctg gtgctgatgc cgaacccgtt ctatcagatc
     3361 tatgaaggcg cggcgctgct cgccggggcc gagccgtact tcctgaacac accggccgga
     3421 accggcttcc tgcccgatta cgacgccgtg ccagaggagg tctgggcgcg ctgccagttg
     3481 ctgtatctct gctcgcccgg caacccgacc ggcgccgtga cgccggccgc gacgctcgcg
     3541 aagctgatcg acaaggccga cgagttcggt ttcgtgatcg ccgccgacga gtgttactcc
     3601 gagatctact tcgacgaagc cgcgccgccg gtcggcctgc tcgaagcctg tgcccggctc
     3661 ggccgccacg actaccgccg ctgcgtggtg ttcaactcgc tgtcgaaacg ctcgaacctg
     3721 cccggcctgc gttcgggctt cgtcgccggc gatgccgacg tgctcgccgc cttcctgcag
     3781 taccgcacct atcacggctg cgcgctgccc ggcagcacgc aggcggcgag cgtcgcggcc
     3841 tggggcgacg aggcgcacgt gcaggccaac cgtgcgctgt accgcgccaa gctcgacgcc
     3901 gtgctcgacg tgctcggccc ggtgctcgac gtgtcacgac ccgaggccgg ctttttcctg
     3961 tggccgcgcg tgcccggcga cgacgagcgc tgggcgcagc agctgttcgc cggacagcac
     4021 gtgacggtgc tgcccgggcg ttatctcgca cgcgacaccg ccctcggcaa tcccggcgcc
     4081 gggcgcgtgc gcatcgcgct ggtcgcgccg ctcgccgact gcaccgaagc cgccatccgc
     4141 atccgccgct gcctcggcgc ctgaggccgc tttcgagaac cccagcccct ttccgcggag
     4201 ctttccatga acaagctgca gaccctgatc gaagacgcct tcgagcgccg cgccgaatac
     4261 tcgccggcca gccacccgct cgaagtccgc caggccgtcg aggaggcgct ggcgctgctc
     4321 gaagccggac aggcgcgtgt cgccgagaaa cagggcaccg gctggcacgt caaccagtgg
     4381 ctgaagaagg ccgtgctgct gtcgttccgc ctcaacgaca accgcgtgct cggcgacggt
     4441 ccgctgcgcg ccttcgacaa ggtgccgacc cgctacggcg actgggacga agcgcgcttt
     4501 cgcgaaggcg gcgcgcgcgt ggtgccgggc gcggtcgtgc gcggcggcag cttcgtcgcg
     4561 aagaacgtcg tgctgatgcc gtcgttcgtg aacatcggcg cttacgtcga cgaaggcacg
     4621 atggtcgata cctgggcgac ggtcggctcc tgcgcgcaga tcggcaggaa cgtgcatctg
     4681 tccggcggcg tcggcatcgg cggcgtgctg gagccgctgc aggccggccc gaccatcatc
     4741 gaggacaact gcttcatcgg cgcgcgctcc gaggtcgtcg agggcgtggt ggtcgaggaa
     4801 ggctcggtga tctcgatggg cgtgttcatc ggccagagca ccaagatcta caaccgcctg
     4861 accggcgagg tcagctacgg ccgcgtgccg gccggctcgg tcgtcgtctc cggctcgctg
     4921 cccgccccgg acggctcgca cagcctgtac tgcgcggtga tcatcaagca ggtcgacgag
     4981 cgcacgcgcg gcaaggtcgg gctgaacgaa ctgctgcgcg actgaggatc gtctgactgc
     5041 tccgccgcct ctgctgcatg ggccggaggg gcggagccgg cgcttcggcg catgtctccg
     5101 tcctgcctgc cgaatcaggg cgatccggac tgactccgcc cggagtagcg caccagacct
     5161 gggacgggcg cctgcgcaag cggtgccgtt gaccggagcg catgttcctc cggctccttc
     5221 gctactcagg tgcgtcgtgt ccggatgccg agcttctgca tccgcgaccg caaggtgctc
     5281 ggatgcagcc cgagggccgc cgccgcgccc ttcggcccct cgatgacgcc gctgcagtgc
     5341 gtcagcacct gctcgatgtg ccggcgctcg acggcttcaa ggttgatgct ggcttcttcc
     5401 ggggcggtcg cgaccgccgc cggcatgggg cgccctgccc cg
//
LOCUS       DAOQJH010000072         5435 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_8700_length_5435_cov_4.773606, whole genome shotgun sequence.
ACCESSION   DAOQJH010000072 DAOQJH010000000
VERSION     DAOQJH010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 5435)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5435)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5435
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8700_length_5435_cov_4.773606"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            56..409
                     /gene="rplR"
                     /locus_tag="PLQ18_02760"
     CDS             56..409
                     /gene="rplR"
                     /locus_tag="PLQ18_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HNE00600.1"
                     /translation="MDKKAARIRRGLRARMKIRELGAVRLVVHRTPKHIYAQIIAPNG
                     SQTLVAASTLEKGVIEGLGFTGNIEAARAVGKAIAEKAKAAGISEVAFDRSGFKYHGR
                     VQALADAAREHGLQF"
     gene            421..930
                     /gene="rpsE"
                     /locus_tag="PLQ18_02765"
     CDS             421..930
                     /gene="rpsE"
                     /locus_tag="PLQ18_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006747239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HNE00601.1"
                     /translation="MATNVESSQNTDGLNEKLIAVNRVAKVVKGGRQFGFTALTVVGD
                     GQGRIGFGYGKAREVPVAIQKAMEKARKNMSTIALAGTTLQHPLNGEHGAARVFMQPA
                     SEGTGIIAGGAMRAVFEVLGVKDVLAKCVGSRNPINVVRATINGLKGMASPDYIAAKR
                     GKKVEEILG"
     gene            934..1119
                     /gene="rpmD"
                     /locus_tag="PLQ18_02770"
     CDS             934..1119
                     /gene="rpmD"
                     /locus_tag="PLQ18_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019557089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HNE00602.1"
                     /translation="MSASKVKVTLVKSTSGRLAAHKACVRGLGLRRMHHSTVVIDTPE
                     NRGMINKVSYLLKVEEA"
     gene            1122..1556
                     /gene="rplO"
                     /locus_tag="PLQ18_02775"
     CDS             1122..1556
                     /gene="rplO"
                     /locus_tag="PLQ18_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HNE00603.1"
                     /translation="MRLNTLKPGEGARQEGKRVGRGIGSGLGKTCGRGHKGQHSRAGG
                     FHKVGFEGGQMPLQRRLPKVGFRSLTARDTAEVRLHELNGIDAEVIDIHALKAAGIVA
                     QPALFAKVVLSGELSRAVTLKGVAVTRGARAAIEAAGGKVEE"
     gene            1562..2887
                     /gene="secY"
                     /locus_tag="PLQ18_02780"
     CDS             1562..2887
                     /gene="secY"
                     /locus_tag="PLQ18_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007040471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HNE00604.1"
                     /translation="MSAPLPSIGKMTELRARLLFLLGALIVFRIGTHIPVPGIDPHAM
                     AQLFQQQRGTILDMFNMFSGGALQRLSIFALGIMPYISASIIVQLMTVIVPTLERLKK
                     EGNAGRRKITQYTRYLTVVLATFQSVGVSIALQGQSAGGLAVVPHPGIGFVAISAITL
                     VSGTLFLMWLGEQVTERGIGNGISMLIFAGIVAGLPAALGGTLELARTGELNALLVIA
                     LLVVALVVTALVIFVERGQRRITVNYAKRQEGRKVYAAQSSHLPLKLNMAGVIPPIFA
                     SSLILFPATIGSWFGNTPGMGWLRDLSSTLAPGHPLYVALYATLIVFFCFFYTALVFN
                     SRETADQLKKSGAFIPGIRPGAQTATYIDRVLTRLTLAGAIYITGVCLLPEFLILYWK
                     VPFYFGGTSLLIIVVVVMDFMAQVQAHLMSHQYESLMKKANFGAGGALR"
     gene            2968..3081
                     /gene="rpmJ"
                     /locus_tag="PLQ18_02785"
     CDS             2968..3081
                     /gene="rpmJ"
                     /locus_tag="PLQ18_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011330914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HNE00605.1"
                     /translation="MKVRASVKKLCRNCKIIRREGVVRVICKDARHKQRQG"
     gene            3174..3530
                     /gene="rpsM"
                     /locus_tag="PLQ18_02790"
     CDS             3174..3530
                     /gene="rpsM"
                     /locus_tag="PLQ18_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HNE00606.1"
                     /translation="MARIAGINIPVNKHAVIALQSIYGIGQTRAKAICAAADVAPETK
                     VKDLVEAQVEALRAEVAKFVVEGDLRREVSMNIKRLMDLGCYRGLRHRRGLPLRGQRT
                     RTNARTRKGPRRAVKK"
     gene            3578..3967
                     /gene="rpsK"
                     /locus_tag="PLQ18_02795"
     CDS             3578..3967
                     /gene="rpsK"
                     /locus_tag="PLQ18_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010869057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HNE00607.1"
                     /translation="MAKPAVKTRKRVKKNVVDGIAHVHASFNNTIITITDRQGNTLAW
                     ATAGGSGFRGSRKSTPFAAQVAAERAGAVVKEFGLKNLEVNVNGPGPGRESAVRALNA
                     QGFKITNIMDVTPIPHNGCRPPKKRRV"
     gene            3982..4602
                     /gene="rpsD"
                     /locus_tag="PLQ18_02800"
     CDS             3982..4602
                     /gene="rpsD"
                     /locus_tag="PLQ18_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HNE00608.1"
                     /translation="MARYIGPKCKLSRREGVDLQLKSAARALESKCNLERAPGQHGAR
                     RGRLSDYGLQLREKQKLRRIYGVLERQFRRYYQEAARRKGSTGENLLRLLETRLDNVV
                     FRMGFGATRSEARQLVSHKAIVVNGEAINVPSYAVKASDVVAVREKSRNQARIQQSLE
                     LAKSRGFADWIDVDTGKMSGVFKRVPDRADLPADINESLVVELYSK"
     gene            4622..>5435
                     /gene="rpoA"
                     /locus_tag="PLQ18_02805"
     CDS             4622..>5435
                     /gene="rpoA"
                     /locus_tag="PLQ18_02805"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017901544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HNE00609.1"
                     /translation="MSGSFDFLKPRIVDVERIDDKRARVTLEPLERGFGHTLGNALRR
                     ILLSSIPGAAVVECEIEGVLHEYSAIEGVQEDVIDLLLNLKGLAIVMHGDTEATLSLR
                     KRGPGPVLAADVQTTHAVEIVNPNHVIATLNKEIELGMKLRVAKGRGYEPATQRLVAE
                     DESRPIGRLQLDASFSPINRVSYKVEAARVEQRTDLDKLVIELETNGTVDPEEAIRAA
                     ARILLDQLSVFVDLKGKEEAEPAPQQVDIDPILLRPVDELELTVRSANCLKAE"
BASE COUNT          976 a         1750 c         1810 g          899 t
ORIGIN      
        1 acgccgacga aaccatcgtc ctcaaagagg cgaagaagaa gtaaggggcc cggaaatgga
       61 caagaaagct gctcgcatcc gccggggcct gcgtgcccgg atgaagattc gcgaactggg
      121 cgccgtgcgc ctggtcgtgc accgtacgcc gaagcacatc tacgcgcaga tcatcgcccc
      181 gaacggcagc cagacgctgg ttgccgcctc gacgctcgaa aagggcgtga tcgagggact
      241 cgggttcacc ggcaacatcg aggccgcccg cgccgtgggc aaggcgatcg ccgagaaggc
      301 caaggccgcc ggcatctccg aggtcgcctt cgaccgctcc ggtttcaagt atcacggccg
      361 tgtgcaggcg ctcgccgatg cggcgcgtga gcacggactg cagttctaag ggttgggtcc
      421 atggctacga atgtcgagtc ttcgcagaat accgatggcc tgaacgagaa gctgatcgcc
      481 gtcaaccgtg tcgccaaggt cgtcaagggc ggccggcagt tcggcttcac ggcgctgacc
      541 gtggtcggcg atggccaggg ccgcatcggc ttcggctacg gcaaggcccg tgaagtgccg
      601 gtcgcgatcc agaaggcgat ggagaaggcg cgcaagaaca tgagcacgat cgcgctcgcc
      661 ggcaccacgc tgcagcaccc gctgaacggc gagcacggcg cggcccgcgt gttcatgcag
      721 ccggcctcgg aaggtaccgg catcatcgcc ggcggtgcga tgcgcgccgt gttcgaggtg
      781 ctcggggtca aggacgtgct ggccaagtgc gtcggttccc gcaacccgat caacgtcgtg
      841 cgcgcgacga tcaacgggct caagggcatg gcctcgccgg actacatcgc cgccaagcgc
      901 ggcaagaaag tcgaagagat cctggggtga gccatgtccg ctagcaaggt gaaggtcacg
      961 ctcgtcaaga gcaccagcgg tcggctggcc gcacacaagg cttgtgtccg cggtctcgga
     1021 ctgcgccgca tgcatcacag caccgtcgtg atcgataccc cggaaaaccg gggcatgatc
     1081 aacaaggtgt cgtatctgct gaaggtcgag gaggcctgag catgcgcctg aatacgctga
     1141 agccgggcga aggcgcccgg caggaaggca agcgcgtcgg ccgtggcatc ggttccggtc
     1201 tgggcaagac ctgcggccgc ggccacaaag gtcagcactc gcgtgccggt ggcttccaca
     1261 aggtcggttt cgaaggcggt cagatgccgc tgcagcgccg gctgccgaag gtcggcttcc
     1321 gttcgctgac ggcgcgcgat accgccgagg tgcgcctgca cgagctcaac ggcatcgatg
     1381 ccgaagtcat cgacatccac gccctgaagg cggccggcat cgttgcgcag ccggctctct
     1441 tcgcgaaggt cgtgctgtcc ggtgaactga gccgtgccgt cacgctcaag ggcgtggccg
     1501 tcaccagggg tgcgcgcgcc gcgatcgaag cggcgggcgg caaggtcgaa gagtaagtcc
     1561 catgtcggca ccgctgccct cgatcggcaa gatgaccgaa ctgcgcgccc gcctgctgtt
     1621 cctgctcggc gcgctgatcg tgttccgcat cggtacccac atcccggtgc ccggcatcga
     1681 cccgcacgcg atggcgcagc tgttccagca gcagcgcggc acgatcctgg acatgttcaa
     1741 catgttctcg ggcggcgcgc tgcagcggct gtcgatcttt gcgctcggca tcatgccgta
     1801 catctccgct tccatcatcg tgcagctgat gacggtgatc gtgccgacgc tggagcggct
     1861 gaagaaggaa ggcaatgccg ggcggcgcaa gatcacgcag tacacgcgct acctgacggt
     1921 cgtgctggcg accttccagt cggtcggcgt cagcatcgcg ctgcaggggc agagcgccgg
     1981 cggtctggcg gtggtgccgc atccgggcat cggcttcgtg gcgatctcgg cgatcacgct
     2041 ggtgtcgggc acgctgttcc tgatgtggct gggcgaacag gtcaccgagc gcggcatcgg
     2101 caacggcatc tcgatgctga tcttcgccgg catcgtcgcc ggcctgccgg cagccctcgg
     2161 cggcacgctc gaactcgccc gcaccggcga gctgaacgcg ctgctggtga tcgcgctgct
     2221 ggtcgtggcg ctggtggtga ctgcgctggt gatcttcgtc gagcgcggcc agcggcgcat
     2281 caccgtcaac tacgccaagc gccaggaagg ccgcaaggtc tatgcggcgc agtcgagcca
     2341 tctgccgctg aagctcaaca tggccggcgt gatcccgccg atcttcgcgt cgagcctgat
     2401 cctgttcccg gccacgatcg gctcgtggtt cggcaatacg ccgggcatgg gctggctgcg
     2461 tgatctcagt tcgacgctgg ccccgggtca tccgctgtac gtggcgctgt atgcgacgct
     2521 gatcgtgttc ttctgcttct tctacaccgc gctggtgttc aactcgcggg aaaccgcaga
     2581 ccagctgaag aagtcgggcg cgttcattcc gggcatccgt ccgggggcgc agacggccac
     2641 ctacatcgac cgggtgctga cgcggctgac gctggccgga gcgatctaca tcaccggcgt
     2701 gtgcctgctg cccgagttcc tgatcctgta ctggaaggtg ccgttctact tcggtggcac
     2761 ctcgctgctg atcatcgtgg tcgtggtcat ggacttcatg gcccaggtcc aggcccacct
     2821 gatgtcgcac cagtacgaaa gtctgatgaa gaaggccaat ttcggcgccg gtggcgccct
     2881 gcgctgaccg cgccggcatc ttcaggccag tcaaccaatg atttggcgac cgcagcacag
     2941 atgatgccgg ccgccgggag aatccagatg aaagttcgtg cctccgtcaa gaagctctgc
     3001 cgcaactgca agatcatccg tcgtgaaggc gtggtgcgcg tgatctgcaa ggacgcccgc
     3061 cacaagcagc gtcagggctg atcgctacgc cgttgcccga attcgggaaa cggtttattc
     3121 ttcggcgttt cacgcgccag cctttgcgcg tacgaaattt tgggagtttc ggcatggccc
     3181 gtattgcagg catcaacatt ccggtgaaca agcacgcggt gatcgcgttg cagtccatct
     3241 acggcatcgg ccagacgcgc gcgaaggcga tctgtgcggc tgccgatgtg gcgccggaga
     3301 ccaaggtcaa ggatctggtc gaggcacagg tcgaggcgct gcgcgccgag gtcgcgaagt
     3361 tcgtcgtcga aggcgatctg cggcgcgaag tgtccatgaa catcaagcgt ctcatggacc
     3421 tcggctgcta tcgcggcctg cgtcaccgtc gcggcctgcc gctgcgcgga cagcgcacgc
     3481 gcaccaacgc ccgcacccgc aagggcccgc gccgcgccgt caagaagtaa gccgccgcga
     3541 ccggcgaccc accgtatcag gctgacaggt atccttcatg gccaagcccg ctgtgaagac
     3601 gcgcaagcgc gtcaagaaga acgtcgtcga cggcattgcg cacgtccacg cgtcgttcaa
     3661 caacacgatc atcaccatca ccgaccgcca gggcaacacg ctggcatggg ccaccgccgg
     3721 tggttccggt ttccgcggct cgcgcaagag cacgccgttc gctgcgcagg tcgcggccga
     3781 gcgcgccggt gcggtcgtca aggaattcgg cctcaagaat ctcgaggtca acgtcaacgg
     3841 gcccggtccc ggccgcgagt cggccgtgcg tgcgctgaac gcgcagggct tcaagatcac
     3901 gaacatcatg gatgtcacgc cgattccgca taacggctgc cgtcccccga agaagcgccg
     3961 ggtctaacga ggaatatcgt cgtggcgaga tacatcggtc cgaagtgtaa gctgagccgt
     4021 cgcgaaggcg tcgacctgca gctcaagtcc gctgcccgcg cgctcgaatc caagtgcaac
     4081 ctggagcgtg cccccggtca gcatggcgcc cgtcgcggtc gcctgtcgga ttacggcctg
     4141 cagctgcgcg agaagcagaa gctgcgccgc atctacggcg tgctggagcg gcagttccgt
     4201 cgctactatc aggaagccgc gcgccgcaag ggctcgaccg gcgaaaacct gctgcgcctg
     4261 ctggagacgc gtctcgacaa cgtcgtcttc cgcatgggct tcggtgcgac ccgctccgaa
     4321 gcgcgccagc tggtgtcgca caaggccatc gttgtcaatg gcgaggcgat caacgtgccg
     4381 tcctacgccg tcaaggcctc cgacgtggtc gcggtgcgcg agaagtcgcg caatcaggcg
     4441 cgcatccagc agtcgctgga actcgccaag agccgtggct tcgccgactg gatcgacgtg
     4501 gacaccggca agatgagcgg cgtgttcaag cgcgtgccgg atcgtgccga tctgcctgcc
     4561 gacatcaacg agtcgctggt cgtcgagctc tactcgaagt aatcccgggg gaagatcgca
     4621 gatgtcgggc tcctttgatt ttctgaagcc gcgcatcgtc gatgtcgaac gcatcgatga
     4681 caagcgcgcg cgggtgacgc tcgaaccgct ggaacgcggt ttcggccaca ccctcggcaa
     4741 tgccctgcgt cggatcctgc tgtcgtcgat tccgggtgcc gcggtcgtgg agtgcgagat
     4801 cgaaggcgtg ctgcatgagt actcggcgat cgagggcgtg caggaagacg tcatcgacct
     4861 gctgctgaac ctcaaggggc tcgccatcgt catgcatggc gacaccgagg cgacgctgag
     4921 cctgcgcaag cgcgggccgg gtccggtgct cgccgccgac gtgcagacca cgcacgcggt
     4981 cgagatcgtc aatccgaatc acgtcatcgc gacgctgaac aaggaaatcg agctcggcat
     5041 gaagctgcgg gtcgccaagg gccgcggcta cgagccggcg acgcagcgcc tggttgccga
     5101 ggacgagagc cgtccgatcg gccggctgca gctcgatgcc tcgttcagcc cgatcaaccg
     5161 cgtcagctac aaggtcgagg cggcgcgcgt cgaacagcgc accgacctcg acaagctggt
     5221 catcgaactc gagaccaacg gcacggtgga tccggaagag gcgatccgtg ctgcggcccg
     5281 catcctgctc gatcagctgt cggtgttcgt cgatctcaag ggcaaggaag aagccgagcc
     5341 ggcgccgcag caggtcgaca tcgacccgat cctgctgcgc ccggtcgacg agctcgagct
     5401 gaccgtgcgt tcggccaact gcctgaaggc cgaaa
//
LOCUS       DAOQJH010000073         5413 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_8752_length_5413_cov_4.994401, whole genome shotgun sequence.
ACCESSION   DAOQJH010000073 DAOQJH010000000
VERSION     DAOQJH010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 5413)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5413)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5413
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8752_length_5413_cov_4.994401"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1626)
                     /locus_tag="PLQ18_02810"
     CDS             complement(<1..1626)
                     /locus_tag="PLQ18_02810"
                     /inference="COORDINATES: protein motif:HMM:NF015410.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF294 nucleotidyltransferase-like
                     domain-containing protein"
                     /protein_id="HNE00610.1"
                     /translation="MEPRPQLSRIPPFDRLPAETLARVAEAVDVLFFRRDQTVLTEQE
                     APDSLYVLTQGSVQVLAGGEVIARYGPEDCFDLEALLRRRSPGRIVAREDTVCLALPR
                     AEFLYLLAECPEFADPLFAELAGRTAGSRPVDTHGELAGFMIAKIGDAYVHPPLQVTA
                     ASSIHDAARAMRDNRATSLLVVREDGEIGICSGTDLRNAVVIERRSPDAPVGDIASYG
                     LVSLEEDDFLFNALLTMTRHSISRVVIRHDGAIRGVLEQVDLLSYLSNHSHLIAVQIE
                     RAGSREELAKASHELTRVIEALHGKGVKLRYIGQLLSELNKKLLGKLFTLIAPPELID
                     NACLVVMGSEGREEQILKTDQDNALLLRDGYTHPGLDAITQAFTEALVAFGYPPCPGN
                     IMVSNPYWTKPLGAFKDEIFNWIKRPSEDGFMHFAIFFDAMPVAGDTALLDEAKRYLH
                     QQLQSNSGFFAHFARATVSFETPLGFFQNFVTGKDGQHRDELDLKKGGIFPLTHGVRS
                     LALEHRLEPTNTLERIEALEAKGVLTPARAAGLT"
     gene            complement(1730..3427)
                     /locus_tag="PLQ18_02815"
     CDS             complement(1730..3427)
                     /locus_tag="PLQ18_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005220420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006847 - plasma membrane acetate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation acetate symporter"
                     /protein_id="HNE00611.1"
                     /translation="MKKLLPASLFASLFLALPAFADAVKGPVEKQAINVTAIVMFLAF
                     VAATMGITYWAASRTKTAKDFYTGGGGITGFQNGLAIAGDYMSAASFLGISGLVFSSG
                     YDGLIYSIGFLVGWPIIMFVMAEQLRNLGKFTFADVASYRFGQTEIRTMAAFSSLVVV
                     AFYLIAQMVGAGKLIQILFGLDYNLAVVGVGVLMLIYVVFGGMTATTWVQIIKAVLLL
                     SGATFMALAALSHFGFSPEEMFKQAVAGHPKGDKIMGPGGLVTDPINAISLGLALMFG
                     TAGLPHILMRFFTVPNAKEARKSVFYATGFIGYFYILTFIIGFSAIVLLVQHPEFYKA
                     DALVNGAVDKAKLISGLNGGTNMVAIHLANAVGGNIFLGFISAVAFATILAVVSGLTL
                     AGASAISHDLYASVIAKGRSDEVTEMKVSRGATITLGVIAVVLGIIFEKQNVAFMVGL
                     AFAIAASANFPVLIMSVFWSKMTTRGAMIGGWIGLTVAVVLTVISKAIWVDVIGNAAP
                     LITLNNPAIISIPSAFIAIWLFSVTDKSKRAEQDLLGFEAQDVRCQTGIGAEGASGH"
     gene            complement(3427..3735)
                     /locus_tag="PLQ18_02820"
     CDS             complement(3427..3735)
                     /locus_tag="PLQ18_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011388394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HNE00612.1"
                     /translation="MQPDVVRQVKSNPKYHDLVAKRSAFGWTLAVVMLVIYYGFILVI
                     AYSPTSLAAKVGAGATMSIGMPLGVAIIVSAFLLTGIYVARANSEFDRLTHEIVEAVK
                     "
     gene            complement(3954..>5413)
                     /locus_tag="PLQ18_02825"
     CDS             complement(3954..>5413)
                     /locus_tag="PLQ18_02825"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNE00613.1"
                     /translation="VIGMTDLALDAVAEAEVRDYLVTARGAAGNLLAIIDDLLDFSKL
                     EAGRLVTRTEPFLLRDTLRTTITNHARAAAGRGLELLWEVDADVPDAVTGDGERLAQI
                     VSHLLSNAVKFTEQGEIVVRVRAQEVSEQSVVLHCSVRDTGIGIAPDKIARIFQPFVQ
                     GDTSSTRRFGGTGLGLSISQRLAEMMGGRLWVESSPERGSDFQFTLRLERQPARPDVR
                     RGPEWTGMRALVLAASQSARAVLERQLRYWGFQVAAAGVADEAERLLQERAGFGQHFE
                     LALFDETQPGTVELLRRIAPAERRPRVLLLRSPLTPPSASLPAGADATLLKPVDPARL
                     AEALTRALSPAAERTPLVRFGDDTAVTPAPRLDREAIDGRLAPFDPAALLAQCHGNAA
                     LAGRLLDLLRRQIPEHLATLRRAVDSEDLVLIRREVRTLAGPLGSVGAQVALSRLRSL
                     EAADAAHAPACFQLLETELQRVLEFAALPLPAEAG"
BASE COUNT          918 a         1707 c         1926 g          862 t
ORIGIN      
        1 ggtcagcccg gccgcccgcg ccggcgtcag cacgcctttg gcttccagcg cctcgatgcg
       61 ctccagcgtg ttggtcggct ccagccggtg ttcgagcgcg aggctgcgca cgccgtgcgt
      121 cagcgggaag atgccgccct tcttgaggtc gagttcgtcg cggtgctggc cgtccttgcc
      181 ggtcacgaag ttctggaaga agccgagcgg ggtctcgaag ctgacggtcg cgcgcgcgaa
      241 gtgcgcgaag aagccggagt tgctctgcag ctgctggtgc aggtagcgct tggcctcgtc
      301 gagcagggcg gtgtcgccgg cgaccggcat cgcgtcgaag aagatcgcga agtgcatgaa
      361 gccgtcttcg ctcgggcgct tgatccagtt gaagatctcg tccttgaacg cgccgagcgg
      421 cttcgtccag tacgggttcg agaccatgat gttgccgggg cagggcggat agccgaaggc
      481 cacgagggcc tcggtgaagg cctgcgtgat cgcatcgagc ccgggatgcg tgtagccgtc
      541 gcgcagcagc agcgcgttgt cctgatcggt cttgaggatc tgctcctcgc ggccctcgct
      601 gcccatgacc accagacagg cgttgtcgat cagctcgggc ggcgcgatca gcgtgaacag
      661 cttgccgagc agcttcttgt tcagctccga cagcagctgg ccgatgtagc gcagcttcac
      721 gcccttgccg tgcagcgcct cgatcacgcg cgtcagctcg tggctggcct tggccagttc
      781 ctcgcggctg ccggcgcgct cgatctgcac ggcgatcagg tgcgagtggt tcgagagata
      841 agacagcagg tcgacctgtt cgagcacgcc gcggatcgcg ccgtcgtggc ggatcacgac
      901 gcggctgatg ctgtggcggg tcatggtcag cagcgcgttg aacaggaagt cgtcttcttc
      961 cagcgacacc aggccgtagg aggcgatgtc gccgaccggc gcatccggcg agcggcgttc
     1021 gatgacgacg gcgttgcgca ggtccgtgcc cgagcagatg ccgatctcgc cgtcctcgcg
     1081 cacgaccagc agcgaggtcg cgcggttgtc gcgcatcgcg cgcgcagcgt cgtggatcga
     1141 gcttgcggcc gtcacctgca gcggcgggtg gacgtaggca tcgccgatct tggcgatcat
     1201 gaagccggcc agctcgccgt gcgtgtcgac cgggcggctg ccggcggtgc gtccggcgag
     1261 ttccgcgaac agcggatcgg cgaattccgg gcattcggcg agcaggtaca ggaattcggc
     1321 gcgcggcagc gccaggcaga ccgtgtcctc gcgcgcgacg atgcgcccgg ggctgcgccg
     1381 gcgcagcagc gcttcgagat cgaagcagtc ctcggggccg tagcgcgcga tgacctcgcc
     1441 accggcgagc acctgcaccg agccctgggt cagcacatac agcgaatcgg gcgcttcctg
     1501 ctcggtcagc accgtctggt cgcggcggaa gaacagcaca tcgaccgctt cggcgacgcg
     1561 ggcgagcgtc tcggcgggca gacgatcgaa gggcgggatg cggctcagct gcggacgcgg
     1621 ttccatgaca tttccgtaat gaaaaacccc gccagtctaa aagagccggc ggggtcctgg
     1681 ggagcgttcc ggagcctttc ggccccggat ggcttgactt ggatcaggct cagtggccgg
     1741 aagcgccttc ggcaccgatg ccggtctggc agcgcacgtc ctgggcctcg aagccgagca
     1801 ggtcctgttc ggcgcgcttg ctcttgtcgg tgaccgagaa cagccagatc gcgatgaacg
     1861 ccgacgggat cgagatgatc gccgggttgt tcagcgtgat cagcggtgcg gcattgccga
     1921 tgacatcgac ccagatggcc ttggagatga cggtcagcac caccgcgacg gtcaggccga
     1981 tccagccgcc gatcatcgcg ccgcgcgtcg tcatcttcga ccagaacacc gacatgatca
     2041 gcaccgggaa gttggcgctg gccgcgatcg cgaaggccag accgaccatg aacgcgacgt
     2101 tctgcttctc gaagatgatg ccgaggacga cggcgatgac gccgagcgtg atggtcgcgc
     2161 cacgcgagac cttcatttcg gtgacttcgt cggagcggcc cttggcgatg acgctcgcgt
     2221 acaggtcgtg cgagatcgcc gaggcaccgg ccagcgtcag gcccgagacc accgcgagga
     2281 tggtcgcgaa cgcgacggcc gagatgaagc cgaggaagat gttgccgccg acggcgttgg
     2341 cgaggtggat cgcgaccatg ttggtgccgc cgttgaggcc gctgatcagc ttggccttgt
     2401 cgacggcgcc gttgaccagc gcgtcagcct tgtagaactc ggggtgctgg acgagcagca
     2461 cgatcgccga gaagccgatg atgaaggtca ggatgtagaa gtagccgatg aagccggtcg
     2521 cgtagaacac cgacttgcgg gcttccttgg cgttcggcac cgtgaagaag cgcatcagga
     2581 tgtgcggcag gccggcggtg ccgaacatca gcgcgagacc cagcgagatc gcgttgatcg
     2641 gatcggtgac cagaccgccc gggcccatga tcttgtcgcc cttcgggtga ccggcgaccg
     2701 cctgcttgaa catttcttcc ggcgagaagc cgaagtgcga cagggctgcc agcgccatga
     2761 acgtggcacc ggagagcagc agcacggcct tgatgatctg cacccaggtg gttgcggtca
     2821 tgccgccgaa caccacgtag atcagcatca gcacgccgac gccgacgacg gcgaggttgt
     2881 aatcgagacc gaacaggatc tggatcagct tgccggcacc gaccatctgc gcgatcaggt
     2941 agaacgcgac gacgaccagc gacgagaaag cggccatcgt gcggatttcg gtctggccga
     3001 aacggtacga ggccacgtcc gcgaacgtga acttgccgag attgcgcagc tgttcggcca
     3061 tcacgaacat gatgatcggc cagccgacga ggaagccgat cgaatagatc aggccgtcat
     3121 agccggacga gaacaccaga cccgaaatac cgaggaacga ggccgccgac atgtaatcgc
     3181 cggcaatcgc gaggccgttc tggaaaccgg tgatgccgcc gccgccggtg tagaagtcct
     3241 tggcggtctt ggtgcgcgag gccgcccagt aggtgatgcc catcgtcgcg gccacgaagg
     3301 ccaggaacat cacgatggcg gtgacgttga tcgcctgttt ctcgaccggg cccttgacgg
     3361 catcggcaaa ggcgggcagt gcgaggaaca gcgaggcgaa caggctggca gggaggagct
     3421 tcttcatcac ttgaccgcct cgacgatttc gtgggtcaga cggtcgaact cggagttcgc
     3481 gcgcgcgacg tagataccgg tcagcaggaa cgccgagacg atgatcgcaa cgccgagcgg
     3541 catgccgatg ctcatcgtgg caccggcacc gactttcgca gcgagcgagg tcggtgaata
     3601 ggcgatcacg agaatgaagc cgtagtagat gaccagcatc acgaccgcaa gcgtccagcc
     3661 aaatgcggag cgcttggcga cgagatcgtg atatttcgga ttgctcttaa cctgtcggac
     3721 gacatcgggt tgcatggcgc attccttatt tatggctctg attcagaaag tcgctctccg
     3781 gcccgtccgc accgtttcgc atccgacctc ctggatgcgg cggcactcat cttcctgatt
     3841 ttcgttgcag atcaggatag tgagatgacg gtctgggccg ccgcggagtt tgaccgaacg
     3901 caggggggga agtaaaggcc ggtgacgttt acgtcaactt gcggtcgggc cgcctaaccg
     3961 gcctcggccg gcagcggcag cgcggcgaat tcgagcacgc gctgcagttc ggtctccagc
     4021 agctggaaac aggccggtgc atgcgcggca tcggcggctt cgaggctgcg caggcgcgag
     4081 agcgcgacct gcgccccgac cgagccgagc ggtccggcca gcgtgcgcac ctcgcgccgg
     4141 atcagcacca gatcctcgct gtcgacggcg cggcgcagcg tcgccagatg ctcgggaatc
     4201 tggcggcgca gcaggtcgag cagccgcccg gcgagggcgg cgttgccgtg gcactgcgcg
     4261 agcagtgctg cgggatcgaa gggggcgagc cgcccgtcga tcgcctcgcg gtccaggcgc
     4321 ggagccggcg tgaccgctgt gtcgtcgccg aagcgcacca gcggcgtgcg ctcggccgcc
     4381 gggctgagcg cccgggtgag ggcttcggcg aggcgcgcgg ggtcgaccgg cttcagcagc
     4441 gtcgcatcgg ctccggccgg caggctggcg gagggcggtg tcagcggcga gcgcagcagc
     4501 agcacgcgcg gccggcgttc ggcgggcgcg atccggcgca gcagctcgac ggtgccgggc
     4561 tgggtttcgt cgaacagcgc gagttcgaaa tgctggccga agccggcgcg ctcctgcagc
     4621 aaccgctcgg cctcgtcggc aacgccggcc gctgccacct ggaagcccca gtagcgcagc
     4681 tggcgttcga gcacggcgcg tgcgctctgg ctcgcggcca gcaccagcgc gcgcatgccg
     4741 gtccattcgg ggccgcggcg cacgtcgggc cgggcgggct ggcgttcgag ccgcagcgtg
     4801 aactggaagt cgctgccgcg ctcggggctg ctctcgaccc agagccggcc gcccatcatc
     4861 tcggcgagcc gctgcgagat gctcaggccg agcccggtgc cgccgaagcg gcgcgtgctg
     4921 gaggtgtcgc cctgcacgaa gggctggaag atgcgcgcga tcttgtccgg cgcgatgccg
     4981 atgccggtgt cccgcaccga gcagtgcagc acgaccgact gttcggagac ttcctgcgcg
     5041 cgcacgcgca cgacgatctc gccctgttcg gtgaacttga ccgcattcga cagcaggtgc
     5101 gaaacgatct gtgccagccg ttcgccgtcg ccggtgacgg catccggcac gtcggcatcg
     5161 acctcccaca gcagttcgag gccgcgcccg gcggcggcgc gcgcgtggtt ggtgatggtc
     5221 gtgcgcagcg tgtcgcgcag caggaagggt tcggtgcgcg tgacgaggcg cccggcttcg
     5281 agcttcgaga agtccagcag gtcgtcgatg atcgcgagca ggttgccggc cgctccgcgg
     5341 gcggtgacca gataatcgcg cacctcggct tcggcgacgg catcgagcgc cagatcggtc
     5401 atgccgatca cgg
//
LOCUS       DAOQJH010000074         5413 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_8753_length_5413_cov_3.883165, whole genome shotgun sequence.
ACCESSION   DAOQJH010000074 DAOQJH010000000
VERSION     DAOQJH010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 5413)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5413)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5413
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8753_length_5413_cov_3.883165"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..684
                     /locus_tag="PLQ18_02830"
     CDS             <1..684
                     /locus_tag="PLQ18_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018150464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HNE00614.1"
                     /translation="VVHSLTKYLGGHGTSIGGVLVDSGKFPWAEHKERFRRLNEPDVS
                     YHGVTYTEALGPAAYIGRARVVPLRNMGAAISPFNSFLVLQGIETLALRMDRHCANAL
                     AVAGFLRDHAAVEWVGYPGLPDSPFRPLVDRYMGGKGSGLVTFGVKGGREAGARFQDA
                     LQLVTRLVNIGDAKSLACHPASTTHRQLNAAELAKAGVSEDMVRLSIGIEHIDDILAD
                     LDQALAATR"
     gene            738..1331
                     /locus_tag="PLQ18_02835"
     CDS             738..1331
                     /locus_tag="PLQ18_02835"
                     /inference="COORDINATES: protein motif:HMM:NF012774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HNE00615.1"
                     /translation="MLHRHLVAWLRSFFVSDFGRPARGPAPLFAALAFVLVVIGVLVL
                     QPWGMSSSAGPAPVGATEDCTVRSVQDGDTVTLACNAQERRVRLWGIDAPELGQGDWG
                     RLARSGLTARLRKENVRLEVLDTDQYGRTVGRIWLGKTDIGLEQVHAGRAAVYREFNN
                     SSEYTTAEAEARRAGRGIWEAPGAQQTPWLWRRLNPR"
     gene            1496..2572
                     /locus_tag="PLQ18_02840"
     CDS             1496..2572
                     /locus_tag="PLQ18_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013031550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNE00616.1"
                     /translation="MLPTCTADPSGGVRLDGSWTLAGFACGLPLLPEKPVPRIDAGGL
                     TALDTAGALQILALEAGSDTHWDGLTAGHATLLDLVRAQAARERPPPPPVPGCLEALG
                     RRATAQLCEVRDFLGFFGEMSLAFWRALLLPHRFRWTPFLANIQSAGLAAMPIVGLLS
                     FLLGVVIAYQGGSQLGRYGANIFIVELVSLTMLREMSVIMTAIIVAGRTGSAWTAQIG
                     TMRVTEEIDALRTIGIRPMDMLVIPKVFALMVALPLLTVYADAMGVLGGMVMADLQLG
                     VSFQEFLQRFPQAVSVRSFLIGVGKAPVFALLIALVGCYQGFKVSGGAEAVGRQTTVS
                     VVQSIFLVIVVDAAFSVLFSWLKI"
     gene            2557..3372
                     /locus_tag="PLQ18_02845"
     CDS             2557..3372
                     /locus_tag="PLQ18_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNE00617.1"
                     /translation="MAEDLTPPPSPPPVGDAPPVIEIRGLGTRFGAQVVHEDLELTVR
                     RGEIVGLVGGSGSGKTTLLREVALLDAPSAGSVRLFGAETVGLDESEAFALRRRFGMM
                     FQHGALFSSLTVRENVAVPLHEHTTLPEAEIDRLAELKVVLAGLPPGSGDKYPSQLSG
                     GMIKRAGVARALALDPDLLFLDEPTAGLDPVGAAALDELVLELRAALGLTVLIVTHDL
                     DTLWRITDRVAFLGERRVLACEPIESLSRRPHPLIRQYFCGPRARAAEAAAED"
     gene            3394..4317
                     /locus_tag="PLQ18_02850"
     CDS             3394..4317
                     /locus_tag="PLQ18_02850"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HNE00618.1"
                     /translation="MENKVNYTLVGVFVLALSAALIGTLLWLTVGTEEKQYDIYRLFF
                     RDSVAGLNPKAAVRLRGVAVGEVAGIALDRKQPDRVIVDLKIERGTPILESTKAVLAT
                     QGLTGIAAIELSVDNPNAPPLKRREDEPFPVIPTGPSLVQRLDNAVTQVLGDFGKLSN
                     RLQALLDQDNLDAVHDTLGNLRTLSAALAKQTATLDQVLRATADTAEAASGAAKEIRT
                     LGARLKTTVGLVDDTVRSFGQTARTLDTAVAGTRRDLQQFAGSAGPELAGLLAELRRM
                     TEVVERLGGELERNPRMLLFGRKNDNPGPGE"
     gene            4353..4988
                     /locus_tag="PLQ18_02855"
     CDS             4353..4988
                     /locus_tag="PLQ18_02855"
                     /inference="COORDINATES: protein motif:HMM:NF015823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-type transport auxiliary lipoprotein family
                     protein"
                     /protein_id="HNE00619.1"
                     /translation="MTRVRLTLLALLLGGCSAINLGSSPDAPRTIWLLSAVPAAAPAG
                     AARPAGVLLVDKPEARAGFDTPRMAYSRQPLAIDYYTRSEWADVPARMLQPLIVQTLA
                     QGGTWTAVLPATNPGRADVRLTTNVLELVHDLSAGEPGVVRLRLRAVFTDVVDRRVLA
                     TREFAAVEPAASANAEGYAAAADRADARVLAGLAAFTAETFAPAPARVGRR"
     gene            5172..>5413
                     /locus_tag="PLQ18_02860"
     CDS             5172..>5413
                     /locus_tag="PLQ18_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006788022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE00620.1"
                     /translation="MPSIRMFHCRFSCRTAIRPWWPAALLGLLGLLAGGLPPAQAGTV
                     EPIRIGSVLTLSGPGAALGEPELKTLQLYVERLNAAG"
BASE COUNT          759 a         1920 c         1926 g          808 t
ORIGIN      
        1 gtcgtgcact cgctgaccaa gtacctcggc ggccacggca ccagcatcgg cggcgtgctg
       61 gtcgattcgg ggaaatttcc ctgggccgag cacaaggaac gcttccgccg cctgaacgag
      121 ccggatgtct cctatcacgg cgtgacctac accgaggcgc tcggcccggc ggcctacatc
      181 ggccgcgcgc gcgtcgtgcc gctgcgcaac atgggcgcgg cgatctcgcc gttcaacagc
      241 ttcctggtgc tgcagggcat cgagacgctg gcgctgcgca tggaccggca ctgcgcgaat
      301 gcgctcgcgg tcgccggatt cctgcgcgat cacgcggcgg tcgagtgggt cggctatccg
      361 ggcctgcccg acagcccgtt ccggccgctg gtcgatcgct acatgggcgg caagggctcg
      421 ggcctcgtga ccttcggcgt caagggcgga cgcgaggccg gtgcgcgctt ccaggatgcc
      481 ttgcagcttg tgacgcggct ggtcaacatc ggcgacgcca agagcctggc ctgccatccg
      541 gcgagcacca cgcaccgcca gctcaacgcc gcggaactgg cgaaggccgg cgtcagcgag
      601 gacatggtgc ggctgtcgat cggcatcgag cacatcgacg acatccttgc cgacctcgac
      661 caggcgctgg ccgcgacgcg ctgaagcgcc gggggcgggc cgccggacac aggccttcca
      721 gggagattcc gaacacgatg ctgcaccgtc acctcgtcgc ctggctgcgc agtttcttcg
      781 tcagcgattt cggccggccg gcgcgcggtc cggcgccgct gttcgcggcg ctcgccttcg
      841 tgctcgtcgt catcggcgtg ctggtgctgc agccctgggg catgagcagc agcgccggac
      901 cggcgccggt cggggcgacc gaggactgca cggtgcgctc ggtgcaggac ggcgacaccg
      961 tcacgctcgc ctgcaacgct caggagcggc gcgtgcggct ctggggcatc gacgccccgg
     1021 aactcggcca gggcgactgg gggcggctcg cgcgcagcgg cctgacggcg cggctgcgca
     1081 aggagaacgt gcgcctcgaa gtgctggaca ccgaccagta cgggcgcacg gtcgggcgca
     1141 tctggctcgg gaagaccgac atcggtctcg aacaggtcca cgccgggcgc gccgccgtct
     1201 atcgcgagtt caacaacagc tccgaataca cgaccgccga agccgaggcc cggcgcgccg
     1261 ggcgcggcat ctgggaagcc cccggggcgc agcagacgcc gtggctgtgg cggcggctga
     1321 atccgcgatg aatccgcgag gagggtccgg gcggaatccg ggcggagtcc atgccgggtc
     1381 cgtgctgatg cggtgctgaa tccgtgccga tgcgcggccc ggcagccgcc gtcgcgtggc
     1441 ttggcgcccg tgcggcgccc gtgaaacact cgaagccatg acgactgccg agctcgtgct
     1501 tcccacctgc accgccgacc cgtccggggg cgtgcgtctg gacggcagct ggacgctcgc
     1561 cggctttgcc tgcggcctgc cgctgctgcc ggaaaagccg gtgccgcgca tcgacgccgg
     1621 cggcctgacc gcgctcgaca ccgccggggc gctgcagatc ctcgcgctcg aagccggcag
     1681 cgacacgcac tgggacggcc tgaccgcagg gcacgcgacg ctgctcgatc tggtgcgcgc
     1741 ccaggccgcg cgcgagcgcc cgccgccgcc gccggtgccg ggctgcctcg aagcactcgg
     1801 ccggcgcgcg acggcgcagc tctgcgaggt gcgggatttc ctcggtttct tcggcgagat
     1861 gagcctcgcg ttctggcgcg cgctgctcct gccgcaccgg ttccgctgga cgccgtttct
     1921 cgccaacatc cagagcgcgg ggctcgcggc gatgccgatc gtcgggctgc tgtccttcct
     1981 gctcggcgtc gtcatcgcct atcagggcgg ctcgcagctc gggcgctacg gcgccaacat
     2041 cttcatcgtc gagctggtca gcctgacgat gctgcgcgag atgtcggtga tcatgaccgc
     2101 gatcatcgtc gccgggcgca ccggctcggc gtggaccgcg cagatcggca cgatgcgcgt
     2161 gaccgaggag atcgacgcgc tgcgcaccat cggcatccgg ccgatggaca tgctggtgat
     2221 cccgaaggtg ttcgcgctga tggtcgcgct gccgctgctg accgtgtacg ccgatgcgat
     2281 gggcgtgctc ggcggcatgg tgatggccga cctgcagctc ggcgtgagct tccaggagtt
     2341 cctgcagcgc tttccgcagg cggtgtcggt gcgctccttc ctgatcggcg tcggcaaggc
     2401 gccggtgttc gcgctgctga tcgcgctggt cggctgctat cagggcttca aggtctcggg
     2461 cggcgccgag gcggtcggcc ggcagacgac cgtctcggtc gtgcagtcga tctttctggt
     2521 catcgtcgtc gatgcggcgt tctcggtgct gttctcgtgg ctgaagatct gacgccgccg
     2581 ccatcaccac caccggttgg cgacgcgccg ccggtcatcg agatccgcgg cctcggcacg
     2641 cgcttcggtg cgcaggtcgt gcatgaagac ctcgaactga ccgtgcgtcg cggcgagatc
     2701 gtcgggctgg tcggcggcag cggcagcggc aagaccacgc tgctgcgcga ggtcgcgctg
     2761 ctcgatgccc cctcggccgg cagcgtgcgg ctgttcggcg ccgagaccgt cggtctcgac
     2821 gagagcgaag ccttcgcgct gcgccggcgc ttcggcatga tgttccagca cggcgcgctg
     2881 ttctcgtcgc tgaccgtgcg cgagaacgtc gccgtgccgc tgcacgagca cacgacgctg
     2941 cccgaggccg agatcgaccg gctcgcggag ctgaaggtcg tgctcgccgg cctgccgccg
     3001 ggcagcggcg acaagtatcc gtcgcagctc tcgggcggca tgatcaagcg cgccggcgtg
     3061 gcgcgcgcac ttgcgctcga tccggacctg ctgttcctcg acgagccgac cgccggcctc
     3121 gacccggtcg gcgccgcggc gctcgatgaa ctggtgctgg aactgcgtgc cgcgctcgga
     3181 ctcacggtac tgatcgtcac gcacgatctc gacacgctct ggcgcatcac cgaccgcgtt
     3241 gccttcctcg gcgagcggcg cgtgctcgcc tgcgagccga tcgaatcgct gtcgcggcgc
     3301 ccgcatccgc tgatccgcca gtacttctgc ggtccgcgcg cgcgggccgc cgaggcagcc
     3361 gccgaagact gagccgcgcg caggaggcag ccgttggaga acaaggtcaa ctacacgctg
     3421 gtcggggttt tcgtgctggc cctgtcggcg gcgctgatcg gcacgctgct gtggctgacg
     3481 gtcggcaccg aggaaaagca gtacgacatc taccggctgt tcttccgcga ctcggtcgcc
     3541 ggcctgaacc cgaaggcggc ggtgcgcctg cgcggcgtgg cggtcggcga ggtcgcgggc
     3601 atcgcgctcg atcgcaagca gcccgatcgc gtcatcgtcg atctgaagat cgagcgcggc
     3661 acgccgatcc tcgaaagcac gaaggccgtg ctcgcgaccc agggcctgac cggcatcgcc
     3721 gcgatcgagc tgtcggtcga caacccgaac gcaccgccgc tgaagcgacg cgaagacgag
     3781 ccgtttccgg tgatcccgac cgggccgtcg ctggtgcagc gcctcgacaa cgccgtgacc
     3841 caggtgctcg gggacttcgg caagctgtca aaccggctgc aggcgctgct cgatcaggac
     3901 aacctcgatg ccgtccacga cacgctcggc aacctgcgca cgctcagtgc ggcgctcgcg
     3961 aagcagaccg ccacgctcga tcaggtgctg cgcgccaccg ccgataccgc cgaggccgcg
     4021 agcggcgcgg cgaaggagat ccgcaccctc ggcgcgcgcc tgaagaccac ggtcggcctc
     4081 gtcgatgaca ccgtgcgcag tttcggccag accgcacgca cgctcgacac ggcggtcgcc
     4141 ggcacccggc gcgacctgca gcagttcgcc ggcagcgccg gccccgaact cgccggcctg
     4201 ctcgccgagc ttcggcgcat gaccgaggtc gtcgagcgcc tcggcggcga actcgaacgc
     4261 aatccgcgca tgctgctgtt cgggcgcaag aacgacaatc cggggccggg cgaatgaatg
     4321 ccgccggggc ctgcatgaag cgaggactgc cgatgacccg tgtccgcctc acgctgctcg
     4381 cgctgctgct cggcggctgc tcggcgatca atctcggcag ctcgccggat gcaccgcgca
     4441 cgatctggtt gctgtcggcc gtgccggcgg ccgcaccggc cggcgcggcg cgcccggccg
     4501 gcgtgctgct ggtcgacaag cccgaggccc gcgccggctt cgacacgccg cgcatggcgt
     4561 attcgcgcca gccgctggcg atcgactact acacccgcag cgaatgggcc gacgtaccgg
     4621 cgcgcatgct gcagccgctg atcgtgcaga cgctcgcgca gggcggcacc tggacggcgg
     4681 tgctgccggc cacgaatccg ggccgtgccg acgtgcgcct gacgacgaac gtgctcgaac
     4741 tcgtgcacga tctgtcggcc ggcgagccgg gcgtcgtgcg gctgcgcctg cgggccgtgt
     4801 tcaccgatgt cgtcgaccgg cgcgtgctgg cgacgcgcga attcgccgcc gtggagccgg
     4861 cggcgagtgc gaatgccgag ggctacgcgg cggcggccga tcgcgccgat gcccgcgtgc
     4921 tcgccgggct cgcggcgttc acggccgaaa ccttcgcgcc ggcaccggca cgtgttggcc
     4981 ggcgctgagc gccatccggc atccggcatc cggcatccgc aagcgcgcag taccgcgatt
     5041 ccgggcagta ccgtagtacc gcaatgcctt ttcagccgta gcgaagacca gacccgaaac
     5101 acgggcaaat ggcggcgtgc cagtgacggg cgccaaccat ccagggatac cagggaaaga
     5161 ggagaaaacc gatgcccagc attcgcatgt tccactgtcg gttcagctgc cgcacagcga
     5221 tccggccgtg gtggccggca gcgctgctcg gtctgctcgg tctgctcgct ggcggcctgc
     5281 cgccggcgca ggccggcacc gtcgagccga tccgcatcgg ttcggtgctg acgctgagcg
     5341 gccccggcgc cgcacttggc gagccggagc tgaagacgct gcagctctac gtcgagcgcc
     5401 tgaacgccgc cgg
//
LOCUS       DAOQJH010000075         5393 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_8809_length_5393_cov_3.776508, whole genome shotgun sequence.
ACCESSION   DAOQJH010000075 DAOQJH010000000
VERSION     DAOQJH010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 5393)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5393)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5393
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8809_length_5393_cov_3.776508"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            422..886
                     /locus_tag="PLQ18_02865"
     CDS             422..886
                     /locus_tag="PLQ18_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013714502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNE00621.1"
                     /translation="MNTQTQNTQTQNTQTHSEVLAVLETWLGAIRAGDLDAIGRHYAA
                     DVSAYDAVGPLRFQGRPAYAEHWRRCLEFCEGETIYEPQPPAVFADSQLAVVHFLLRC
                     GKRKADGSEESSWMRTTLGLRREAGSHWLIFHEHSSIPFDMESGQTQFGLMP"
     gene            996..1406
                     /locus_tag="PLQ18_02870"
     CDS             996..1406
                     /locus_tag="PLQ18_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018388369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNE00622.1"
                     /translation="MKLQPYVFFNGDAEAAAHFYRDALGAGITMLMRYRDCPETPDPA
                     HVPPGNADKVMHMSLSIGDTVLMGADDCSGSGRGFAGFSLSLSVTEVAEAEKAFAALA
                     DGGSVCVPLGPTFFSPAFGMLNDRFGVSWMIVVM"
     gene            complement(1513..2145)
                     /locus_tag="PLQ18_02875"
     CDS             complement(1513..2145)
                     /locus_tag="PLQ18_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015861052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HNE00623.1"
                     /translation="MFENIQHWIAEYGYLTILIWTFIEGETVVIVAGVIAKLGHLELP
                     LVMLSAFVGSLCGDQFYFFIGRKQGRKLLDKHPKWAGRVAWINRHLERHRDFLVLTFR
                     FYYGVRNITPFVIGASDISTVRFIVLNMIGAAVWAISFAGAGYLFGEAMETFLGRMHN
                     AQLYILGTIIGLAVLAWLFMRLRRRAKDRQIEADAVVQAPEAKSPPAPDA"
     gene            complement(2279..3706)
                     /locus_tag="PLQ18_02880"
     CDS             complement(2279..3706)
                     /locus_tag="PLQ18_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012970928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HNE00624.1"
                     /translation="MPGSNPLLRVLLTCLVLLAAGRAPAVELPDFTTLVEQYAPAVVS
                     VSARTAAKDAADEPDEDKPGKPPVQNPFEGTPFEEPFRRFFEQMPHGNSPRPRELPAS
                     IGSGFVISADGYVLTNAHVVEGADTVTVGLRDRTELIAKVIGADKRSDVALLKIDGAA
                     PLPVVRLGDAEQLKVGQWVLAIGSPFGFESSATAGIVSALGRSLPSDSYVPFIQTDAA
                     VNPGNSGGPLFNFNGEVVGINSQIYSRSGGYQGLSFAIPINVAMDVAEQLKTKGRVAR
                     GWLGVLIQEVTPDLAKSFGLDRARGALIGQVLPEGPALKAGLKPGDIVLAFNGRPLLR
                     SSELPPLVGSYLPGKDATLSVFRDGRETTITVKIEELPDDARQAGKAGSGTRRGLGLT
                     LGELPPEAARKGETGVVVREVAPGPAARAGIRTGDVIVRLDNQPVTGVAQFEELAAKL
                     GKGKPVPVLVRRGESPLFLALTPPE"
     gene            complement(3741..4910)
                     /locus_tag="PLQ18_02885"
     CDS             complement(3741..4910)
                     /locus_tag="PLQ18_02885"
                     /inference="COORDINATES: protein motif:HMM:NF026257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HNE00625.1"
                     /translation="MPPTSRPPPERRPTTLLLALFCGSIPDPAHLQGADWEQLLPYAR
                     RHEALGRLATVLAARGVLDRVPARVRFHLDSARVRAQSRMRRLRWEALNLRLVLPGVP
                     VLVLKGVACALCGLPSVQGRLSADLDVLVPKARIAEAEAGLLAGGWEHAAHSDYDDAY
                     YREWMHELPAFHHAHRGTQLDLHHGILPPTARLHPDPALLLAAAEPVPGVPDLYVPCA
                     SDLLLHAAVHACYDGAFAQPLRDLLDLHGLITDLGDETFWQALPARAVALDLARPLWY
                     ALTLVQRELGTPVPAAVFTALAAHAPPPQLAGVLPALVHAVLHERPMAPLAALALYIR
                     SHWLRMPPRLLLPHLARKAVQRLRTRLGVAAPGAPAAAVEVATPAEPPRPPPGQP"
     gene            5154..>5393
                     /locus_tag="PLQ18_02890"
     CDS             5154..>5393
                     /locus_tag="PLQ18_02890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE00626.1"
                     /translation="MQDHEHSGTDGQDAAAPVSLRRRRLLKSAAVVAVPAVLTLHAGY
                     ARAANSSAVRCALNTVNGTGIRITNENTPGLYKEMG"
BASE COUNT          844 a         1825 c         1960 g          764 t
ORIGIN      
        1 gtcgccgaca gcccggcgtt caggcgccgc agcgccaggc cgatcgtcac gccgaacagg
       61 ccggcggcga gcggggccgg atcgctgccg tgcaggccga gcgtgcgcca gccgtaatcg
      121 acgccgagca ccagcgtgat gccggcagcg atcgtcagcc agtaggtgcg gtaggacatg
      181 ggaagcggct gcaggtcgtg ggaagcgtcg aaaggtcgtg ccggggccat ccgtccgcag
      241 ccggtgccgc agaagaccct gcacgaagtg tcgtgcatcc ggcggcggga cggcgcggtt
      301 tcggtgaggt ccgggggccg gcggacgggg cgccaggatt tctgcggaac cggagcgctg
      361 cgcaggccgt cggcatccat gcccgtgcaa gccccgcaac cacgatccgg gagataccga
      421 gatgaacacg cagacccaga acacgcagac ccagaacaca cagacccatt ccgaagtgct
      481 ggccgtgctc gaaacctggc tcggcgcgat ccgcgccggc gacctcgacg cgatcggccg
      541 ccactatgcc gcggacgtga gcgcctacga tgccgtcggt ccgctgcgct tccagggccg
      601 cccggcttac gccgaacact ggcgtcgctg cctggagttc tgcgagggcg agacgatcta
      661 cgagccgcag ccgccggccg tgttcgccga cagccagctc gccgtcgtgc acttcctgct
      721 gcgctgcggc aagcgcaagg ccgacggcag cgaggaaagc tcgtggatgc gcacgacgct
      781 cggcctgcgc cgcgaggccg gcagccactg gctgatcttc cacgagcaca gttcgattcc
      841 gttcgacatg gaatccggcc agacccagtt cgggttgatg ccttgaagcg gcacggtcac
      901 ggggccctgc catgctggcg ggcaccgggc cgggccagcg ggttcaacgc gctggagcgg
      961 ctccgtcatg cccggacgcc agcgaggaga gaacgatgaa actgcagccc tatgtgttct
     1021 tcaacggcga tgccgaggcg gccgcccact tctatcgcga tgcgctcggc gccggaatca
     1081 cgatgctgat gcgctatcgc gactgcccgg aaacgcccga tccggctcat gtgccgccgg
     1141 gcaacgccga caaggtcatg cacatgagcc tgagcatcgg tgacaccgtg ctgatggggg
     1201 ccgatgactg cagcggcagc ggccggggtt tcgccggctt ctcgctgtcg ctgagcgtca
     1261 ccgaggtcgc cgaggccgaa aaggccttcg cggcgctggc ggacggcggc agcgtgtgcg
     1321 tgccgctcgg cccgaccttc ttctcgccgg ccttcggcat gctgaacgac cgcttcggcg
     1381 tgtcgtggat gatcgtcgtg atgtgaggcg ccggggctgc agcgcaggcg cccgcgtcag
     1441 gctgcgcttg cccggaacga agccgcccgc cgctcgtggg gcgggcggtg cggcgatgcc
     1501 ggcgggcgcg gttcaggcgt ccggggccgg aggggacttg gcttcggggg cctgcacgac
     1561 ggcatcggct tcgatctgcc ggtccttcgc gcgccggcgc agccgcatga acagccacgc
     1621 gagcaccgcg agcccgatga tcgtgccgag gatgtacagc tgcgcgttgt gcatgcggcc
     1681 gaggaaggtt tccatcgcct cgccgaacag gtagccggcg ccggcgaagc tgatcgccca
     1741 gaccgcggca ccgatcatgt tcagcacgat gaagcgcacc gtggagatgt cgctcgcgcc
     1801 gatcacgaac ggcgtgatgt tgcgcacgcc gtaatagaac cggaaggtca gcaccaggaa
     1861 gtcgcggtgg cgttcgaggt ggcggttgat ccaggccacg cgcccggccc acttcggatg
     1921 cttgtcgagc agcttgcggc cctgcttgcg gccgatgaag aagtagaact ggtcgccgca
     1981 gaggctgccg acgaaggccg acagcatcac cagcggcagt tcgaggtgtc cgagcttggc
     2041 gatgacgccg gcgacgatga cgacggtttc tccttcgatg aacgtccaga tcagaatcgt
     2101 cagatagccg tattcggcta tccagtgctg gatgttttca aacatgcgca cgcactcggg
     2161 ctgtcggtag gagcaggggc ggcggaccgc gcgggggcgc cattgtgtcg gagcctccgt
     2221 gacgcgtcca tgacgcggcg ccgcagtgcg gtcctgcgct gcggcgccgt gctgcggctc
     2281 actcgggcgg cgtcagtgcc aggaacagcg gggattcgcc gcgcctgacc agcaccggca
     2341 ccggcttgcc cttgccgagc tttgcggcga gttcctcgaa ctgcgcgact ccggtgaccg
     2401 gctggttgtc gaggcgcacg atcacgtcgc cggtgcggat gccggcgcga gcggccggcc
     2461 ccggggcgac ctcgcgcacg accacgccgg tctcgccctt gcgcgcggcc tcgggcggca
     2521 gctcgccgag cgtcaggccg aggccgcgcc gggtgccgct gccggccttg ccggcctgac
     2581 gcgcatcgtc gggcagttcc tcgatcttca cggtgatcgt ggtttcccgg ccgtcgcgga
     2641 acaccgacag cgtcgcgtcc ttgcccggca ggtagctgcc gaccagcggc ggcagctcgc
     2701 tggagcgcag cagcggccgg ccgttgaacg cgagcacgat gtcgccgggc ttcaggccgg
     2761 ccttcagcgc cgggccttcg ggcagcacct ggccgatcag cgcaccgcgc gcccggtcga
     2821 ggccgaagga cttcgccaga tccggcgtga cctcctggat cagcacgccg agccagccgc
     2881 gcgcgacgcg gcccttggtc ttgagctgtt cggcgacatc catcgcgacg ttgatcggga
     2941 tcgcgaacga caggccctga tagccgccgg agcgcgagta gatctgcgag ttgatgccga
     3001 ccacctcgcc gttgaagttg aacagcgggc cgccggagtt gccggggttg accgcggcgt
     3061 cggtctggat gaacggcacg taggagtcac tcggcaggct gcgcccgagc gcgctgacga
     3121 tgccggcggt cgcgctcgat tcgaagccga acggcgagcc gatcgccagc acccactggc
     3181 cgaccttgag ctgctcggca tcgccgaggc gcacgaccgg cagcggcgcg gcaccgtcga
     3241 tcttcagcag cgccacgtcg ctgcgcttgt cggcaccgat caccttcgcg atcagctcgg
     3301 tgcggtcgcg caacccgacc gtcaccgtgt cggcgccctc gacgacgtgg gcgttggtca
     3361 gcacgtagcc gtcggccgag atcacgaagc ccgagccgat cgaggccggc agctcgcgcg
     3421 ggcgcggcga attgccatgc ggcatctgct cgaagaagcg ccggaacggc tcctcgaacg
     3481 gcgtgccctc gaacggattc tggaccggcg gcttgccggg tttgtcctcg tcgggctcgt
     3541 cggcggcgtc cttcgcggcg gtgcgggcgc tgacgctgac cacggccggc gcgtactgct
     3601 cgaccagcgt cgtgaagtcg ggcagctcga cggccggcgc gcggccggcg gcgagcagca
     3661 cgaggcaggt cagcagcacc cgcaggagcg gattcgatcc gggcatctgg gcctccgtag
     3721 ccgtcagtag gcagaaatca tcagggttgt ccggggggcg gccggggcgg ttcggccggg
     3781 gtggcgacct cgacggccgc cgccggtgca ccgggcgcgg ccacgccgag gcgcgtgcgc
     3841 agccgctgca cggccttgcg tgcgaggtgc ggcagcagca ggcgtggcgg catgcgcagc
     3901 cagtgcgaac gaatgtagag cgccagcgcc gccagaggtg ccatcggtcg ttcatgcagc
     3961 accgcatgca ccagcgccgg cagcacgccg gccagctgcg gcggcggggc gtgtgcggcc
     4021 agcgccgtga acacggccgc cggcaccggc gtgccgagtt cgcgctgcac cagcgtcagc
     4081 gcgtaccaga gcggccgcgc cagatcgagc gccacggccc gcgccggcag cgcctgccag
     4141 aaggtctcgt cgccgagatc ggtgatcagc ccgtgcaggt cgagcagatc gcgcagcggc
     4201 tgcgcgaagg cgccgtcgta acaggcatgc acggccgcgt gcagcagcag gtcgctcgcg
     4261 cacggtacat ataaatccgg cacgcccggc accggctcgg ccgctgccag cagcagggcc
     4321 ggatcgggat gcaggcgcgc agtcgggggc aggatgccgt ggtgcagatc gagctgcgtg
     4381 ccgcgatgcg cgtggtggaa ggccggcagt tcgtgcatcc actcgcggta gtaggcgtcg
     4441 tcgtaatcgc tgtgcgcggc gtgctcccag ccgccggcga gcagcccggc ctcggcttcg
     4501 gcgatccgcg ccttcggcac cagcacgtcg agatcggctg acagccgtcc ctgcaccgag
     4561 ggcagcccgc acagcgcaca ggccacgccc ttgagcacca gcaccggcac gccgggcagc
     4621 acgaggcgca ggttcagcgc ctcccagcgc agccggcgca tccgcgactg cgcgcgcacc
     4681 cgcgcgctgt cgagatggaa gcgcacgcgc gccggcacgc gatcgagcac gccccgcgcc
     4741 gcgagcaccg tggccagccg cccgagcgcc tcgtgccggc gcgcgtacgg cagcagttgc
     4801 tcccagtcgg cgccctgcag gtgagccgga tcgggaatcg aaccgcagaa cagcgccagc
     4861 agcagggtgg tcgggcggcg ctcgggcggc gggcgggacg tgggcggcat cggggacagg
     4921 tgggtggcag ttgggaaatt tttacagtgc ccggacggca gcggcagggg tgaagcagac
     4981 ctggttttga attcagtgac ttggggtttt gtgtctgatt ggcttgaaat ctgcataaag
     5041 ccgcacgaga cacagggaaa ctcggctgca agccttgcgg atggcccggt ggagtggcat
     5101 ccgggtgccg attcgtcaga ctataaggca aggccttttg aggacagcaa gcgatgcaag
     5161 accacgaaca ctccggcacg gatggtcagg acgccgcagc cccggtatcc ctgcgccgcc
     5221 gccggctgct gaagtcggct gccgtcgtgg ccgttcccgc ggtgctgacc ctgcacgccg
     5281 gctatgcgcg tgccgcgaac agcagcgccg tgcgctgcgc gctcaacacc gtcaacggta
     5341 ccggcatccg gatcaccaac gagaacaccc ccggcctgta caaggaaatg ggg
//
LOCUS       DAOQJH010000076         5383 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_8832_length_5383_cov_3.559497, whole genome shotgun sequence.
ACCESSION   DAOQJH010000076 DAOQJH010000000
VERSION     DAOQJH010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 5383)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5383)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5383
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8832_length_5383_cov_3.559497"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..898)
                     /gene="lptF"
                     /locus_tag="PLQ18_02895"
     CDS             complement(<1..898)
                     /gene="lptF"
                     /locus_tag="PLQ18_02895"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HNE00627.1"
                     /translation="MLRTFDRFLLRETAAVTAATLLVLLAIVLSSRLATYLTQVASGL
                     LIKEVILQMLVLQAIRFLPVLVPIALMIGVLLVLGRLHQESEMAALTGCGYGPAELYR
                     PLLALALPLAALAALVVLGVAPWAASLQFELQARARQQAQMSLFVPGTFREAAGGQHV
                     VYVESLDPIDGELVKVFIHSTEPDSGRIAVTTGNRGHQETGADGVRYMVLERGFRYEG
                     EPGLTDYRQVHYEHLRVRIDEPASGDVSRKRESVPTAQLLRGDRRDKAELHARIGAIV
                     SIPLIAFVAPLLAKAQPRESRYA"
     gene            1056..2564
                     /locus_tag="PLQ18_02900"
     CDS             1056..2564
                     /locus_tag="PLQ18_02900"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007419258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HNE00628.1"
                     /translation="MEFSVKSGNPEKQRTACLVLGVYESRRLTPPAEQFDTAAEGFLG
                     NVLRRGDLEGKPGQTLLLVNTPATLCDRVLLVGCGRERDLDDRRYKQIIAAAVHTLND
                     TGATEAVSYLTDLNVRGRDVAWKVRLAVEVSEDVLYRYDETRSRKDAPRRPLKRIVLA
                     VPSRRELPLGEQAVREGAAVAEGMKLARDLGNLPPNICTPEYLAQRAALLAGSSPKFA
                     LNVLERADMETLGMGCLVAVARGSRRPPKLIALEWHGAADARQKPVVLVGKGITFDTG
                     GISLKPGPDMDLMKFDMCGAAGVLGTMSAIAALDLPLNVVAVVPAVENMPGGEASRPG
                     DIVTSLSGQTVEILNTDAEGRLVLCDAITWAEQRYEPVTLVDVATLTGACVIALGAHP
                     HGLFSNHGPLANELLDAGRETHDRAWELPLWEDYQDGLDSNFADMANVGPREGGAITA
                     ACFLARYTKKLHWAHLDIAGTAWKTGKQKGATGRPVPLLTRFLINRAAAARD"
     gene            2578..3003
                     /locus_tag="PLQ18_02905"
     CDS             2578..3003
                     /locus_tag="PLQ18_02905"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF016267.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HNE00629.1"
                     /translation="MATRIEFYVLATAGEAACLATACRLVDKARSQDLSVWIRTTSAA
                     QSAAIDRELWTWRRGSFIAHRIHAPGRRPWPVLIGTGAPPESHRALLLNLADDVPDGF
                     EACSRLIEIVDAREDILAASRARYKVYRDRGFAPSAVRL"
     gene            3140..4534
                     /locus_tag="PLQ18_02910"
     CDS             3140..4534
                     /locus_tag="PLQ18_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017775953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNE00630.1"
                     /translation="MSESAPDYRDIIRQVAEHAGHLAIAITDITGHIGEVSERAMREA
                     EAFGTLQGLATHMASQNTAVAEAADSTQRVVGTARSEVDRTRQTIESSLADIRALTES
                     VATIEVQLAELGGAMERVSAVAKGIDAIAKQTNLLALNATIEAVRAGEAGKGFAVVAS
                     EVKELAKQTRSATAEIDVTLRELGERTRGLLAQGAQSKTRSEEVRRGTQAIQQVMDTM
                     GAAMQDVDDQTRRIGDAVHEIAGSCQQTVTALNQVAESVHSSSANLRQARDQLTSVQA
                     VSEALVGTTAVDGIETVDAKFIAMAKDTAKRIGELFEQALTRGDLNESDLFDRQYRPI
                     PGTDPQQHLTRFTDFTDRVLPSLQEALLDRDRRIAFAAAVDDNGYLPTHNRKYSHPQG
                     RDPIWNAANCRNRRIFNDRTGLASGRNQKPFLLQTYRRDLGGGKFVLMKEVAAPIRVR
                     GRHWGGFRLGYIPD"
     gene            4652..>5383
                     /locus_tag="PLQ18_02915"
     CDS             4652..>5383
                     /locus_tag="PLQ18_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012981897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HNE00631.1"
                     /translation="MDKTYEPHAIEQCRYETWESQGRFAPQGSGAPYCIMLPPPNVTG
                     TLHMGHAFQHTIMDALIRYHRMNGDRTLWQPGTDHAGIATQMVVERQLNAEGKSRHDL
                     GREAFLARVWEWKAYSGGTIGRQMRRLGTSPDWSRERFTMDEGLSAAVQEVFVKLYED
                     GLIYRGQRLVNWDPVLHTAVSDLEVVSEEESGSMWLIRYLLADGEDALVVATTRPETL
                     LGDTAVAVNPQDERYRHLIGRSVRLP"
BASE COUNT          935 a         1953 c         1743 g          752 t
ORIGIN      
        1 gtgcatagcg gctctcgcgc ggctgggcct tcgcgagcag cggcgcgacg aaggcgatca
       61 gcgggatcga gacgatcgcg ccgatgcggg catgcagttc ggccttgtcg cgacggtcgc
      121 cgcgcagcaa ctgcgccgtc ggcaccgatt cacgcttgcg gctgacatcc ccggacgccg
      181 gctcgtcgat ccgcacccgc aggtgttcgt aatgcacctg ccggtaatcg gtgagtcccg
      241 gctcgccttc gtagcggaag ccccgctcca gcaccatgta gcgcacgccg tcggcaccgg
      301 tttcctgatg gccgcggttg ccggtcgtga ccgcgatgcg ccctgaatcc ggctcggtgc
      361 tgtggatgaa caccttgacc agttcgccat cgatcggatc gagtgattcg acgtagacga
      421 catgctggcc gccggctgcc tcgcggaagg tgccgggcac gaacaaagac atctgcgcct
      481 gctgacgtgc ccgggcctgc agctcgaact gcagcgaggc cgcccacggc gccacgccga
      541 gcacgaccag cgcggccagc gccgccagcg gcagcgccag tgcgagcagc ggccggtaca
      601 gctcggccgg gccgtagccg cagccggtca gggccgccat ctcgctttcc tgatgcaggc
      661 gccccagcac cagcagcacg ccgatcatca gcgcgatcgg caccagcacc ggcaggaagc
      721 ggatcgcctg cagcaccagc atctgcagga tgacttcctt gatcagcaaa ccgctcgcaa
      781 cctgggtcag gtaggtcgcg agccggctcg acagcacgat cgccagcagc acgagcagcg
      841 tcgcggcggt gactgcggcg gtctcgcgca gcaggaatcg gtcgaatgtg cgcagcaagt
      901 tcggggattc ctcagccgcc ggagcggcgt tgtcggttca aaaaccttcc ggctacactc
      961 ggtcgcccgt ccggccaccg ggccggcgac gggcagcgca gcggaagcac aagcacggcc
     1021 ggcacggccg cccccagcag agtcggagcg caaagatgga attctcggtc aagagcggca
     1081 atcccgaaaa gcagagaacc gcctgtctcg tcctcggcgt ctacgaatcc cgccgcctga
     1141 caccgcccgc cgaacagttc gacaccgctg ccgaaggctt tctcggcaac gtgctgcgcc
     1201 gcggcgacct cgaaggcaag ccgggccaga cgctgctgct cgtgaacacg ccggcaacgc
     1261 tctgcgaccg cgtgctgctg gtcggctgcg ggcgtgagcg cgacctcgat gatcgccgct
     1321 acaagcagat catcgccgcc gccgtgcata cgctcaacga caccggcgcg accgaagccg
     1381 tcagctatct gaccgacctg aacgtacgcg ggcgcgatgt cgcctggaag gtgcgcctcg
     1441 ccgtcgaggt cagcgaggac gtgctctacc gctacgacga gacccgcagc cgcaaggatg
     1501 ccccacgccg gccgctcaag cgcatcgtgc tcgccgtgcc gagccgccgc gagctgccgc
     1561 tcggcgagca ggcggtgcgc gaaggcgcgg ccgtcgccga gggcatgaag cttgcgcgcg
     1621 atctcggcaa cctgccgccg aacatctgca cgcccgaata tctcgcccag cgcgccgcgc
     1681 tgctcgccgg cagctctccg aagttcgcgc tgaacgtgct ggagcgcgcc gacatggaaa
     1741 ccctgggcat gggctgcctg gtcgcggtcg cccgcggcag ccgccggccg cccaagctga
     1801 tcgcgctcga atggcatggc gcagccgatg cccggcagaa gccggtcgtg ctggtcggca
     1861 agggcatcac cttcgatacc ggtggcatct cgctgaaacc cggcccggac atggatctga
     1921 tgaagttcga catgtgcggt gccgccggcg tgctcggcac gatgtcggcc atcgccgcgc
     1981 tcgacctgcc gctgaacgtc gtggccgtgg tgccggccgt cgagaacatg ccgggcggcg
     2041 aggcctcgcg ccccggcgac atcgtcacca gcctgtcggg ccagaccgtc gaaatcctca
     2101 acaccgacgc cgaaggccgg ctggtgctct gcgatgcgat cacctgggcc gaacagcgct
     2161 acgagccggt gacgctggtc gatgtcgcga cgctgaccgg tgcctgcgtg atcgccctcg
     2221 gcgcgcatcc gcacgggctg ttctcgaacc acggcccgct cgccaacgag ctgctcgacg
     2281 ccggccgcga aacccatgac cgcgcctggg agctgccgct gtgggaggac taccaggacg
     2341 gcctcgacag caatttcgcc gacatggcca acgtcggtcc gcgcgagggc ggcgcgatca
     2401 cggctgcctg cttcctcgcg cgctacacga agaagctgca ctgggcgcat ctcgacatcg
     2461 ccggcaccgc ctggaagacc ggcaagcaga agggcgcgac cggccggccg gtgccgctgc
     2521 tgacccgctt cctgatcaac cgcgctgccg ccgcgcgcga ctgaacctgc cgccgccgtg
     2581 gcgacgcgca tcgagttcta cgtgctcgcc accgccggcg aggcggcctg cctcgccacc
     2641 gcctgccggc tggtcgacaa ggcccggtcg caggacctgt ccgtgtggat ccgcaccacc
     2701 tccgcagccc agtcagcggc gatcgaccgc gaactctgga cctggcgccg cggcagcttc
     2761 atcgcccatc gcatccacgc ccccggacgc cggccctggc cggtgctgat cggcaccggc
     2821 gcaccgccgg aaagccatcg cgcgctgctg ctgaatctcg ccgatgacgt gccggacggt
     2881 ttcgaggcct gttcacggct gatcgaaatc gtcgatgccc gcgaagacat cctcgccgcc
     2941 tcgcgtgcgc gctacaaagt ttatcgggac cggggctttg cgccctcggc cgtacggctg
     3001 tagaagcctg ccggtaccgc gtgcctgcca ttgctcaagc tggccggacc cgcggccgat
     3061 atggcatcca ccgacataac cagacggcga cgccgccccc ccggcagtgg ccgcaggcat
     3121 ccaccgagga gcttcattca tgagcgagag cgcgccggac tatcgcgaca tcatccgcca
     3181 ggtcgccgaa catgccggcc acctcgcgat cgcgatcact gacatcacgg gccacatcgg
     3241 tgaggtcagc gaacgcgcga tgcgcgaggc cgaagccttc ggcacgctgc aggggctggc
     3301 gacgcacatg gccagccaga acaccgccgt cgccgaagcg gccgacagca cgcagcgcgt
     3361 ggtcggcacg gcgcgctccg aggtcgaccg cacccgccag accatcgaat cctcgctcgc
     3421 cgacatccgg gcgctgaccg aatcggtcgc gaccatcgag gtccagctcg ccgaactcgg
     3481 cggcgcgatg gagcgcgtca gcgccgttgc caagggcatc gatgcgatcg ccaagcagac
     3541 caacctgctg gcgctgaacg ccacgatcga agccgtgcgc gccggcgagg ccggcaaggg
     3601 tttcgcggtg gtcgcaagcg aggtcaagga actcgccaag cagacccgct cggccaccgc
     3661 cgagatcgac gtgacactgc gcgagctcgg cgaacgcacg cgcggcctgc tcgcccaggg
     3721 cgcgcagagc aagacgcgct ccgaggaagt ccgccgcggc acccaggcga tccagcaggt
     3781 catggacacc atgggcgcgg caatgcagga cgtcgacgac cagacccgcc gcatcggcga
     3841 tgccgtccac gagattgccg gcagctgcca gcagaccgtc acggcgctga accaggtcgc
     3901 cgaatccgtg cattcgtcga gcgcgaacct gcgccaggcg cgcgatcagc tgaccagcgt
     3961 gcaggcggtc agcgaggcgc tggtcggcac gacggccgtc gacggcatcg agaccgtcga
     4021 tgcgaaattc atcgcaatgg ccaaggacac cgcgaaacgc atcggcgaac tgttcgagca
     4081 ggcgctgacc cgcggggacc tgaacgaaag cgacctgttc gaccgccagt accggccgat
     4141 tcccggcacc gatccgcagc agcacctgac ccgcttcacg gacttcaccg accgggtgct
     4201 gccgagcctg caggaagcgc tgctcgatcg tgaccgtcgc atcgccttcg ccgccgccgt
     4261 cgacgacaac ggctatctgc cgacccacaa ccgcaagtat tcgcacccgc aggggcgcga
     4321 tccgatctgg aacgccgcca actgccgcaa ccgtcgcatc ttcaacgacc gtaccggtct
     4381 cgcttcaggt cgaaaccaga agccgttcct gctgcagacc taccggcgtg atctcggcgg
     4441 cggcaagttc gtgctgatga aggaagtcgc cgcaccgatc cgcgtgcgcg gccggcactg
     4501 gggcggattc cggctcggtt acatcccgga ctgaacctgt ccctgccttc gaagctccga
     4561 cgaagccggc cctgcgccgg cttcgtcgtc cctgcccctt cgctatactg cgccgctttc
     4621 ccgacgccgc gccgccagaa ccgaccccgc catggacaag acctacgaac cccacgcgat
     4681 cgaacaatgc cggtacgaga cctgggaatc ccagggccgg ttcgcaccgc agggcagcgg
     4741 cgcaccgtac tgcatcatgc tgccgccgcc gaacgtcacc ggcacgctgc acatgggcca
     4801 cgcgttccag cacaccatca tggacgcgct gatccgctat caccgcatga acggtgaccg
     4861 cacgctgtgg cagccgggca ccgatcatgc cggcatcgcc acgcagatgg tcgtcgaacg
     4921 ccagctcaat gccgaaggca aaagccgcca cgatctcggc cgcgaggcct tcctggcccg
     4981 cgtctgggaa tggaaggcct actcgggcgg tacgatcggc cggcagatgc gccggctcgg
     5041 cacctcgccg gactggagcc gtgaacgctt cacgatggat gaaggcctgt cggccgccgt
     5101 gcaggaagtc ttcgtcaagc tgtacgagga cggcctgatc taccgcggcc agcggctcgt
     5161 gaactgggat ccggtgctgc acaccgccgt ctccgacctc gaagtcgtct ccgaggaaga
     5221 aagcggttcg atgtggctga tccgctatct gctcgccgat ggcgaagatg cgctggtggt
     5281 cgccaccacc cgccccgaga ccctgcttgg cgataccgcg gtcgcagtca acccgcagga
     5341 cgagcgttac cgtcatctga tcggccgctc ggtgcggtta ccg
//
LOCUS       DAOQJH010000077         5332 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_8947_length_5332_cov_3.817510, whole genome shotgun sequence.
ACCESSION   DAOQJH010000077 DAOQJH010000000
VERSION     DAOQJH010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 5332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5332
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8947_length_5332_cov_3.817510"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            228..740
                     /locus_tag="PLQ18_02920"
     CDS             228..740
                     /locus_tag="PLQ18_02920"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HNE00632.1"
                     /translation="MATNIVIMGVCGAGKSLIAGRLAAALGLPHIEGDAFHTCANIAR
                     MSAGVPLTDAERVEWLDQLAGQLGAAALAGSGAVLSCSALKQCYRERLRRGCEGYGLQ
                     FVYLRGSREQLAERLAHRHGHFMPVTLLDSQLADLEEPLPDERAIVCDITATPAQIVA
                     GIVDRLSAQA"
     gene            776..1342
                     /locus_tag="PLQ18_02925"
     CDS             776..1342
                     /locus_tag="PLQ18_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014197390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HNE00633.1"
                     /translation="MKPLCSQRPGGATEALMVVALALMVALVFGNVVARHVFSTGFAA
                     AEELARLLFVWLVFLGAIVAQREQRHLGLDLLQARLPGPLRRLCAVISHALMLWALAL
                     FAEGSWQQSVIGLRTYSTVLGFPQLLVAGSGLVSALAMAGLVARNLWWILAHDPRACV
                     PGQPAVADDSDSDSDSDSDAHGRTGTGQ"
     gene            1339..2616
                     /locus_tag="PLQ18_02930"
     CDS             1339..2616
                     /locus_tag="PLQ18_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014820165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HNE00634.1"
                     /translation="MTLLLFLAVLFGALALGMPIAFALILGGAALMLQLEVFDAQLVA
                     QNLIAGVDSYPLMAVPFFILAGELMNAGGLSRRIVALAIAGVGHVRGGLGYVVIATAM
                     LLASLSGSAIADTAALTTLLLPMMRAHGYPLAGSAGLIAAGGIIAPIIPPSLPFILYG
                     VSTSTSISSLFLAGIVPGLLMGVVLVLVWRRRLAGFGCIPQPPAPARERRRALLEAGW
                     ALLLPVLILGGLRGGWFTPTEAAVVAAVYALVAGCVLYRELDRGALYSAFVRSARTTG
                     VVMFLCAAATVSSYMITLAELPGQLAGPLAPLLAHPRLLMAVIVGLLLLVGMVMDLTP
                     SILILAPVLLPLARSAGIDPVYFGVMFVLVGSIGLITPPVGTVLNVVGGVGGVPMLQT
                     VRGVLPYVGAYCALLGLFVLFPGLITAPLQWLR"
     gene            2789..3790
                     /locus_tag="PLQ18_02935"
     CDS             2789..3790
                     /locus_tag="PLQ18_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006387969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HNE00635.1"
                     /translation="MPVLPFTASADVKPRLIRFGYGLAEDSNQGRAARFFAEEVGRLS
                     GGRLRVRTFGGASLGTDIQMQNALIGGAQEMMVGSTATLVGIVRDFGVFDLPFLFDDE
                     RIADAVLDGPFGTRLLDTLPAQGLIGLVYWENGFRSVTNTRRPIERLEDLHGLKLRVM
                     QNPVYLDFFGRTGANPVPLPFSELFTALETRAVDGQENPVNTIRSSRFYEVQKYLSLT
                     RHVYSPWILLVSRLWWDKLSGDERALLREAAVAARDFERRDSRAEAGQALAQLQAQGM
                     LVNTPSTAELERLRAAVAPVVEAWAAQASPGLVAQLRQAIDAARAAQEPQAAQGTRP"
     gene            complement(3772..5040)
                     /gene="glgC"
                     /locus_tag="PLQ18_02940"
     CDS             complement(3772..5040)
                     /gene="glgC"
                     /locus_tag="PLQ18_02940"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005965381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HNE00636.1"
                     /translation="MENQTATPRYVSRLTRETMALVLAGGRGTRLYELTQWRAKPAVY
                     FGGKFRIIDFPLSNCVNSGIQRIGVLTQYKAHSLIRHLIAGWGSVLSRELGGGIEILP
                     ASQRTSDDWYAGTADAIYQNLDILRVRDPSYILVLSGDHIYKMDYGPMIAAHVESGAA
                     MTVSCIEIPLAEASSFGIISVDAASRVTAFNEKPQQADPMPGRTDVALGSMGNYVFNT
                     EFLFEKLIEDAKDPNSSHDFGKDVIPAIIRDHHVHAYLFRDPATGRLGYWRDVGTVDA
                     FWEANIELAAIQPELNLYDHHWPILTYQSQLPPAKFVFEEPGRTGSAPNSLVSGGCLI
                     SGATVRHSVLFSNVRVMDHALVEDAVILPDVVISPGARIRRAVIDRGCTIPEGMVIGE
                     DLTQDAKQFRVTQRGIVLVTKEMLGQGRVP"
     gene            complement(5075..>5332)
                     /locus_tag="PLQ18_02945"
     CDS             complement(5075..>5332)
                     /locus_tag="PLQ18_02945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE00637.1"
                     /translation="HGTDGRELIVLLNAGPAMDFRLPANAGDGWQVAFDSSAFEAAPG
                     PAAVVHALPARSLRVLSRVPAGTAPASSDSPPVPGRARKPG"
BASE COUNT          699 a         1834 c         1921 g          878 t
ORIGIN      
        1 gccggcgtgc acgggacgga agctggcagg gcggccccgt ggccctgcga agcattttgc
       61 agtccgcaac catccgggcc ttgaccggcc acgggatgat agcgctaaca tagcggcaag
      121 cgtcgtgata tccgttcccc ctccccccga cggcgcctgc ggaccgcccg tttcccccag
      181 aggggcggtc cgtttttttt tgcctttctg aaggtggcgt gaccggcatg gcgaccaaca
      241 tcgtgatcat gggcgtctgt ggcgccggca agagcctgat cgccggacgt ctggccgcag
      301 ccctcggtct gccccatatc gaaggcgatg ccttccatac ctgcgccaac atcgcgcgca
      361 tgtccgccgg cgtgccgctg accgatgccg agcgggtcga gtggctcgac cagctcgccg
      421 gccagctcgg ggccgcggca ctcgccggca gcggggccgt gctcagctgc tcggcgctga
      481 agcagtgcta ccgggagcgc ctgcgccgcg gctgcgaagg ctacggcctg cagttcgtgt
      541 acctgcgcgg cagccgcgag cagctcgccg agcgcctcgc gcaccggcac gggcacttca
      601 tgccggtcac gctgctcgac agccagctcg ccgatctgga ggaacccctg ccggacgagc
      661 gggcgatcgt ctgcgacatc accgccacgc ccgcgcagat cgtcgccggg atcgtcgacc
      721 ggctctcggc gcaggcctga tgcgacggcc ctgccggacg gattcttccc gactcatgaa
      781 acccctgtgc agccagcgcc ccggtggcgc caccgaggcg ctgatggtcg tggcgctggc
      841 gctgatggtg gcgctggtat tcggcaacgt cgtcgcacgc cacgtgttca gcaccggctt
      901 cgcggccgcc gaggaactgg cacggctgct gttcgtgtgg ctcgtgttcc tcggcgcgat
      961 cgtcgcgcag cgcgagcagc gccacctcgg cctcgacctg ctgcaggcgc ggctgccggg
     1021 gccgctgcgg cggctctgtg cggtgatcag ccatgcgctg atgctctggg cgctggcgct
     1081 gttcgccgaa ggcagctggc agcagtcggt gatcggcctg cgcacgtact cgaccgtgct
     1141 cggctttccg cagctgctgg tcgccgggtc cgggctggtg tcggcgctgg cgatggccgg
     1201 gctggtcgcg cgcaatctgt ggtggatcct cgcgcacgat ccgcgcgcct gcgtgccggg
     1261 tcagccggcg gtagccgatg acagcgacag cgacagcgac agcgacagcg acgcacacgg
     1321 acgcaccggg accgggcaat gacgctgctg ctgttccttg ccgtgctgtt cggcgcgctg
     1381 gccctcggca tgccgatcgc gttcgcgctg atcctcggcg gtgccgcgct gatgctgcag
     1441 ctcgaagtct tcgatgcgca gctcgtcgcg cagaacctga ttgccggcgt cgacagctat
     1501 ccgctgatgg ccgtgccgtt cttcatcctc gccggcgagc tgatgaatgc cgggggcctg
     1561 tcgcggcgca tcgtcgcgct ggcgatcgcc ggcgtcggcc acgtgcgcgg cggcctcggc
     1621 tatgtcgtga tcgccaccgc catgctgctc gcgagcctgt cgggctcggc gatcgccgat
     1681 accgcggcgc tcacgacgct gctgctgccg atgatgcgcg cgcacggtta tccgctcgcc
     1741 ggctcggccg ggctgatcgc agccggcggc atcatcgcgc cgatcattcc gccgagcctg
     1801 ccgttcatcc tgtacggcgt cagcaccagc acctcgatca gcagcctgtt cctcgccggc
     1861 atcgtgccgg ggctgctgat gggcgtggtg ctggtgctgg tctggcgccg gcggctcgcc
     1921 ggcttcgggt gtattccgca gccgccggcg ccggcgcgcg agcggcgccg ggcgctgctg
     1981 gaggccggct gggcgctgct gctgccggtg ctgatcctcg gcggcctgcg cggcggctgg
     2041 ttcacgccga ccgaagcggc ggtggtcgcg gcggtgtacg cgctcgtggc cggctgcgtg
     2101 ctgtaccgcg agctcgaccg gggggcgctg tactcggcct tcgtgcgcag cgcccgcacg
     2161 accggcgtcg tgatgttcct gtgcgcggcg gcgaccgtgt cctcgtacat gatcacgctc
     2221 gccgaactgc ccgggcagct cgccgggccg ctggcgccgc tgctcgcgca tccgcggctg
     2281 ctgatggcgg tgatcgtcgg gctgctgctg ctggtcggca tggtcatgga cctgacgccg
     2341 agcatcctga tcctcgcgcc ggtgctgctg ccgctggcgc gcagcgccgg catcgacccg
     2401 gtgtacttcg gcgtgatgtt cgtgctggtc ggctcgatcg gcctgatcac gccgccggtc
     2461 ggcacggtgc tgaacgtcgt cggcggtgtc ggcggcgtgc cgatgctgca gaccgtgcgc
     2521 ggcgtgctgc cctacgtcgg cgcctactgc gcgctgctcg ggctgttcgt gctgtttccg
     2581 gggctgatca cggcaccgct gcagtggctg cgctgaacgc gcacggctgg acaagccgta
     2641 acactcgcga catcggctct gtggcctgat tcgtcctccc gacagagccg gcggcatgca
     2701 tcgccggcca tcccgctgga gacgagaggc gcagcacatg aacattcccc ggattgcgcg
     2761 actgctggcg gtcgcctggc tcgcggccat gccggtcctg ccgttcacgg cctcggccga
     2821 cgtgaagccg cggctgatcc gcttcggcta cggtctggcc gaggacagca accagggccg
     2881 tgcggcccgc ttcttcgctg aggaagtcgg ccggctgtcc ggcggccggc tgcgggtgcg
     2941 gaccttcggt ggcgcgagcc tcggcaccga catccagatg cagaacgcgc tgatcggcgg
     3001 cgcacaggag atgatggtcg gctcgaccgc gacgctggtc ggcatcgtgc gcgacttcgg
     3061 cgtcttcgac ctgcccttcc tgttcgacga cgagcgcatc gccgatgccg tgctcgacgg
     3121 gccgttcggc acgcgcctgc tcgacacgct gccggcgcag gggctgatcg ggctcgtgta
     3181 ctgggagaac ggctttcgca gcgtcaccaa cacccgccgg ccgatcgagc gcctcgaaga
     3241 cctgcacggc ctgaagctgc gcgtgatgca gaaccccgtc tacctcgact tcttcggccg
     3301 caccggcgcc aaccccgtgc cgctgccgtt ctcggagctg ttcacggcgc ttgagacgcg
     3361 cgccgtcgac gggcaggaga acccggtcaa caccatccgc tcctcgcgct tctacgaggt
     3421 gcagaagtac ctgagcctga cccggcacgt gtacagcccg tggatcctgc tggtcagccg
     3481 cctgtggtgg gacaagctgt ccggggacga acgggcgctg ctgcgcgagg cggcggtggc
     3541 ggcgcgcgac ttcgagcggc gcgacagccg cgccgaggcc gggcaggcgc tcgcgcagct
     3601 gcaggcgcag ggcatgctcg tcaacacgcc atcgaccgcc gagcttgaac gcctgcgcgc
     3661 ggcggtcgcg ccggtcgtcg aggcctgggc ggcgcaggcg agtccggggc tggtcgcgca
     3721 gcttcgccag gcgatcgacg cggcccgcgc ggcgcaggag ccgcaggccg ctcagggcac
     3781 ccggccctga ccgagcatct ccttggtcac cagcacgatg ccgcgctgcg tgacgcggaa
     3841 ctgtttcgcg tcctgcgtca ggtcctcgcc gatcaccatg ccttccggaa tcgtgcagcc
     3901 gcggtcgatg acggcgcggc ggatgcgcgc gcccggcgag atcacgacat cgggcaggat
     3961 caccgcgtcc tcgaccagcg cgtgatccat gacgcgcacg ttcgagaaca gcaccgaatg
     4021 gcgcacggtg gccccggaga tcaggcagcc gcccgagacc agcgagttcg gcgcgctgcc
     4081 ggtgcgtccg ggctcctcga acacgaactt cgccggcggc agctgcgact ggtaggtcag
     4141 gatcggccag tgatggtcgt agaggttcag ctccggctgg atcgcggcaa gctcgatgtt
     4201 cgcctcccag aacgcatcga cggtgccgac atcgcgccag tagccgaggc ggccggtggc
     4261 cgggtcgcgg aacaggtagg cgtgcacgtg gtggtcgcgg atgatcgccg ggatcacgtc
     4321 cttgccgaag tcgtgcgagg agttcgggtc cttggcgtcc tcgatcagct tctcgaacag
     4381 gaactcggtg ttgaacacgt agttgcccat cgagccgagc gcgacatcgg tgcgtcccgg
     4441 catcgggtcg gcctgttgcg gcttctcgtt gaaggcggtg acgcggctcg cggcatcgac
     4501 gctgatgatg ccgaagctcg aggcttccgc cagcggaatc tcgatgcacg agacggtcat
     4561 cgccgcgccg gattcgacgt gcgcggcgat catcgggccg tagtccatct tgtagatgtg
     4621 atcgcccgag agcacgagga tgtagctcgg atcgcgcacg cgcaggatgt cgaggttctg
     4681 gtagatcgca tcggcggtgc cggcgtacca gtcgtccgag gtgcgctgcg aggccggcag
     4741 gatctcgatg ccgccgccga gctcgcgcga cagcacgctg ccccagccgg cgatcaggtg
     4801 gcggatcagc gaatgcgcct tgtactgggt cagcacgccg atgcgctgga tgccggagtt
     4861 cacgcagttc gacagcggga agtcgatgat ccggaacttg ccgccgaagt acacggccgg
     4921 cttggcgcgc cactgggtca gctcgtacag gcgtgtcccg cggccgccgg cgagcacgag
     4981 cgccatggtt tcgcgcgtga gccggctgac gtagcgcggg gtggcagtct ggttttccat
     5041 gacgggccct cgattgcgtg gaagcggatg acggtcagcc gggcttgcgg gcacggcccg
     5101 gtaccggcgg ggaatcgctg gaggcaggtg cggtgcccgc cggcacgcgg ctcagcacgc
     5161 gcagcgagcg cgccggcagg gcatgcacga cggcggccgg ccccggcgcc gcctcgaagg
     5221 cactgctgtc gaatgcgacc tgccagccgt cgccggcgtt cgccggcagc cggaaatcca
     5281 tggccgggcc ggcgttgagc agcacgatca gctcgcggcc gtcggtgccg tg
//
LOCUS       DAOQJH010000078         5318 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_8981_length_5318_cov_3.699221, whole genome shotgun sequence.
ACCESSION   DAOQJH010000078 DAOQJH010000000
VERSION     DAOQJH010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 5318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5318
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8981_length_5318_cov_3.699221"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..290)
                     /locus_tag="PLQ18_02950"
     CDS             complement(<1..290)
                     /locus_tag="PLQ18_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019923667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE00638.1"
                     /translation="MFPTDALFPPGFLAEVLADLPSGLAHLSAGADAVSLGSALALLA
                     MSLLSWTLILAKLAGRLARMPARRRAQRVWAETETVAEAEQRLQRTPGAEAWL"
     gene            complement(296..1081)
                     /locus_tag="PLQ18_02955"
     CDS             complement(296..1081)
                     /locus_tag="PLQ18_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HNE00639.1"
                     /translation="MIARPAPLRPAPAALPAAPLSSRPPALRRGLRTGGTFAAALALH
                     VGALGHWTTPETLPQPLPMAPLVVDLIEAVPPPDPLAAAPAETAPPPEPEAPPPEPPP
                     PPEPLPQAITAPPKPPLPAPPKPKPKPVVQPPKPKPTPRPPAPNAARSETPPSPLPPA
                     PVAAAPSTTAANFSAAYLRNPPPRYPPDSRSAGEAGRVLLRVRVTAEGRAESVEIAAS
                     SGHARLDDAALAAVRRWRFVPAQQAGAPVAATVTVPILFKLES"
     gene            1513..1992
                     /locus_tag="PLQ18_02960"
     CDS             1513..1992
                     /locus_tag="PLQ18_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020832193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HNE00640.1"
                     /translation="MSSPVRARLRDWGPVALEQLLTVVWLGSLWTVGYLVAPVLFATL
                     PERALAGQVAGRLFTLVAWLGLGCGAALLALALWRWRRAQASLTLVLLPALMLLLVLT
                     GEFGLQPRMAELKALGESGGAMFARLHGIAALLYLAESLLGLGWLVLRVRETLRRAA"
     gene            complement(1976..2269)
                     /gene="yhbY"
                     /locus_tag="PLQ18_02965"
     CDS             complement(1976..2269)
                     /gene="yhbY"
                     /locus_tag="PLQ18_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013034056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HNE00641.1"
                     /translation="MLKQSQKRHLKALAHPLKPVVIVGANGVTPAVLDEVRLALEHHE
                     LIKVRVNAGDREQREALIADIVEATGCELVQRIGHIAAFFRRNPDKPRITLPA"
     gene            2287..2553
                     /locus_tag="PLQ18_02970"
     CDS             2287..2553
                     /locus_tag="PLQ18_02970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE00642.1"
                     /translation="MSEEKGARSVARGSLSVAASPLNGVKFELGVILLVGLLLVPVVG
                     RVFAEPLWQLLALAAYGVAGALWLVLRARRVLGEQARRSAGDGA"
     gene            2543..3163
                     /gene="rlmE"
                     /locus_tag="PLQ18_02975"
     CDS             2543..3163
                     /gene="rlmE"
                     /locus_tag="PLQ18_02975"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019530768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HNE00643.1"
                     /translation="MARSRSSHRWLKEHFDDEFVRRAQAEGWRSRAVYKLQEINERDR
                     LLKPGTTLVDLGAAPGGWSQYAARALGERGRVLALDILPMDALAGVDFIQGDFREETV
                     FAQLLATLAGTPVDLVISDMAPNTSGLKAVDQPKQMYLVELALDFARQTLRPGGDFLV
                     KVFQGEGFDALLKDLRADFGSVATRKPKASRARSAELYLLARNFRS"
     gene            3237..5153
                     /gene="ftsH"
                     /locus_tag="PLQ18_02980"
     CDS             3237..5153
                     /gene="ftsH"
                     /locus_tag="PLQ18_02980"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018953514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HNE00644.1"
                     /translation="MVKNILLWVVIAVVLMSVFNSFGPRTSPSQQLTYSQFLEEVKQG
                     RVAHVSIDGRSISGRTSTGEKFMTYSPETDNKAMIGTLLDNRVDVEGRPPEQQSVWMQ
                     IFISWFPMLLLIGVWVFFLRQMQGGAGGRGAMSFGKSRARMMSEDQIKVTFADVAGCD
                     EAKEEVGELVEFLRDPGKFQRLGGKIPRGVLMVGSPGTGKTLLAKAIAGEAKVPFFSI
                     SGSDFVEMFVGVGASRVRDMFEQAKKHAPCIIFIDEIDAVGRHRGAGLGGGHDEREQT
                     LNQLLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVPLPDVRGREQILK
                     VHMRKVPVGGDVRPSLIARGTPGFSGADLANLVNEAALFAARANKRDVDMDDFEKAKD
                     KIMMGAERKSMVMSEDEKKLTAYHEAGHAIVGRLVPSHDPVYKVSIIPRGRALGVTMF
                     LPEGDRYSFSKQRLESQISSLFGGRIAESIIFGADFVTTGAQNDIERSTDIARNMVTK
                     WGLSERLGPLTYSEDDGEVFLGRSVTRHKNVSDETAHVIDEEIRTIINRNYLRSEQIL
                     RENLDKLHAMADALIKFETIDSEQIDDIMAGRPPREPAEADADQEPPASGSPVSLDKD
                     RAGDGKIGGPAPQH"
     gene            5157..>5318
                     /locus_tag="PLQ18_02985"
     CDS             5157..>5318
                     /locus_tag="PLQ18_02985"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009726785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HNE00645.1"
                     /translation="MRLDCAGKALDLSRPVVMGVLNVTPDSLSDGGYFLRFSDALRRA
                     EAMVAEGAAI"
BASE COUNT          783 a         1707 c         1965 g          863 t
ORIGIN      
        1 agccaggctt cggcaccggg cgtgcgctgc aggcgctgct cggcctcggc gaccgtttcg
       61 gtctcggccc agacgcgctg cgcgcgccgg cgtgccggca tgcgcgccag ccgcccggcg
      121 agcttggcga ggatcagcgt ccacgacagc agcgacatcg ccagcagcgc cagcgccgag
      181 ccgaggctga cggcgtcggc accggcggac aggtgtgcca gacccgaagg cagatcggcg
      241 aggacctcgg cgaggaagcc gggcggaaac agcgcgtcgg tcggaaacat cgggcttaag
      301 actccagctt gaacaggatc ggaacggtga cggtggcggc gaccggcgcg ccggcctgct
      361 gcgccggcac gaaacgccag cggcgcacgg ccgcgagcgc cgcgtcatca aggcgcgcat
      421 ggccgctgct cgcggcgatc tcgaccgact cggcgcggcc ctcggccgtg acgcgcacgc
      481 gcagcagcac gcgcccggcc tcgccggcac tgcgtgagtc gggcgggtag cgcggcggcg
      541 gattgcgcag gtaggcggcg ctgaagttcg cagccgtcgt cgagggcgca gcggcgaccg
      601 gcgccggtgg cagcggcgag ggcggggttt cgctgcgcgc cgcattcggg gccggcggac
      661 ggggggtcgg cttcggtttc ggcggctgca cgaccggctt cggctttggc ttcggcggtg
      721 cgggcagggg aggcttcggc ggcgcagtga tcgcctgcgg cagcggttcg ggcgggggcg
      781 gcggttcggg tggcggcgct tcgggttcgg gtgggggagc cgtctcggcc ggggcagcgg
      841 cgagcgggtc cgggggcggt acggcttcga tcaggtcgac gaccagcggc gccatcggca
      901 gcggctgcgg cagggtttca ggcgtcgtcc agtgaccgag tgcgccgacg tgcagggcca
      961 gggccgcagc gaaggtgccg ccggtgcgca gcccgcggcg cagcgccggt ggtcgcgacg
     1021 acagcggggc ggccggcagg gcggcgggag cggggcgcag tggcgcaggg cgggcgatca
     1081 cggcttcatc cgggcgtcat gaacaggaat gactgcggat gttaatgaga atgcttactg
     1141 tttgtaaatt gtggaatccg gctgcaacga tcgcagaacc ggcggacgag acccggcacc
     1201 cgccagtgtt ccgggggtat caaaacccgg gtgcaacccc cggcgccgga tacggcagcg
     1261 ccgtcgtgcc gtaaccgggc agcaggcatg gcgggtgcgg cgcaggcagc cggccgtcct
     1321 gcccgtcatc cgtgcatacc gcttggctgc gcatcggcgt tgccggcggc gagcccgcaa
     1381 agcacaatcg cccgcgatgt cttccttccg ccccctgcct gccgcccgcc tctgtcgtgc
     1441 cgatgccgtt gacggtcacc ggccgtttgc ctgtcccaag gtcgcgatcg tgtgttccgc
     1501 ggggcgatgc ccatgagcag ccctgtccgg gcgcggctgc gtgactgggg gccggttgcg
     1561 ctggagcagt tgctgaccgt cgtctggctc ggttcgctgt ggacggtcgg ctatctggtc
     1621 gcgccggtcc tgttcgcgac gctgccggag cgcgcgctgg caggtcaggt tgccggccgg
     1681 ctgttcacgc tcgtggcctg gctggggctc ggttgcggtg cggcactgct cgccctcgca
     1741 ctgtggcgct ggcggcgggc gcaggcatcg ctgacgctgg tgctgctgcc ggcgctgatg
     1801 ctgctgctcg tgctgaccgg cgaattcggc ctgcagccgc gcatggcgga gctgaaggcg
     1861 ctcggggaga gcgggggggc gatgtttgcg cgcctgcacg gcatcgcggc gctgctgtat
     1921 ctcgccgaga gcctgctcgg tctgggctgg ctggtcctgc gcgtgcgcga gacgctcagg
     1981 cgggcagcgt gatgcgcggc ttgtccggat tgcgtctgaa gaaggccgcg atgtggccga
     2041 tgcgctggac cagttcgcag ccggtggcct cgacgatgtc ggcgatcagt gcctcgcgct
     2101 gttcgcggtc gccggcgttg acgcgcacct tgatcagctc gtgatgctcc agcgcgaggc
     2161 ggacttcgtc gaggacggca ggcgtcacgc cgttggcgcc gacgatcacc acgggtttca
     2221 gcgggtgggc gagggccttc aggtgacgct tctggctttg cttgagcatg tctgaccgga
     2281 gtggaaatgt ctgaggaaaa aggggcgcgg agtgtagcac gcggctccct gtcggttgcc
     2341 gcgagcccgc tgaacggcgt caagttcgag ctgggcgtga tcctgctggt cggactactg
     2401 ctggtgccgg tggtcgggcg cgtgtttgcc gagccgctgt ggcagctgct ggcgctggcc
     2461 gcttacggcg tcgccggcgc gctgtggctg gtgctgcgcg cgcggcgcgt gctcggcgag
     2521 caggcccgca ggagtgccgg cgatggcgcg tagccgcagc agccaccgct ggctgaagga
     2581 gcacttcgac gatgagttcg tccggcgcgc ccaggccgag ggctggcgct cgcgcgccgt
     2641 ctacaagctg caggaaatca acgagcgcga ccggctgctg aagcccggca cgacgctcgt
     2701 cgatctcggc gccgcgccgg ggggctggtc gcagtacgcg gcgcgcgcgc tcggcgagcg
     2761 cggccgggtg ctcgcgctcg acatcctgcc gatggatgcg ctggccggcg tcgacttcat
     2821 ccagggggat ttccgcgagg agaccgtgtt cgcgcagctg ctcgcgacgc tggccggcac
     2881 gccggtggac cttgtgattt ccgacatggc ccccaatacc agcggtctga aggccgtcga
     2941 ccagcccaag cagatgtatc tggtcgaact cgcgctcgac ttcgcccgtc agacgctcag
     3001 gcccggtggt gacttcctgg tcaaggtgtt ccagggcgag ggcttcgatg cgctgctgaa
     3061 ggatctgcgt gcggatttcg gctcggtggc cacgcgcaag ccgaaggcct cgcgggcgcg
     3121 cagcgccgaa ctgtatctgc tggcccggaa tttccggtcg tagtaacgtc attcttcatc
     3181 cgggcgccgg ggtcggtgtc cggtctttcg cacggcgagg tgccaccttg aacgacatgg
     3241 tcaaaaacat cctgctctgg gtggtgattg ccgtcgtcct gatgtctgtg ttcaacagct
     3301 tcggaccgcg cacgtcgccg tctcagcagc tgacgtattc gcagttcctc gaagaggtca
     3361 agcaggggcg cgtcgcgcac gtgtcgatcg acgggcgctc gatctccggt cgcaccagca
     3421 ccggcgagaa gttcatgacc tacagccccg agaccgacaa caaggcgatg atcggcacgc
     3481 tgctcgacaa ccgcgtcgat gtcgaaggcc ggccgccgga gcagcagtcg gtctggatgc
     3541 agatcttcat cagctggttc ccgatgctgc tgctgatcgg cgtctgggtg ttcttcctgc
     3601 gccagatgca gggcggggcc ggcggacgcg gtgcgatgag cttcggcaag tcgcgggcgc
     3661 gcatgatgag cgaggaccag atcaaggtca ccttcgccga tgtcgccggc tgcgacgagg
     3721 ccaaggagga agtcggcgaa ctcgtcgagt tcctgcgcga tcccggcaag ttccagcgcc
     3781 ttggcggcaa gatcccgcgc ggcgtgctga tggtcggctc gcccggcacc ggcaagacgc
     3841 tgctcgccaa ggcgatcgcc ggcgaggcca aggtgccgtt cttctcgatc tccggttccg
     3901 acttcgtcga gatgttcgtc ggcgtcggtg cctcgcgcgt gcgcgacatg ttcgagcagg
     3961 ccaagaagca cgcgccgtgc atcatcttca tcgacgagat cgacgcggtc ggccgtcacc
     4021 gcggcgccgg tctcggcggc gggcatgacg agcgcgagca gacgctgaac cagctgctgg
     4081 tcgagatgga cggcttcgag ggcaacgagg gcatcatcgt catcgccgcg accaaccgcc
     4141 cggacgtgct cgatccggcg ctgctgcgcc ccggccgctt cgaccggcag gtggtcgtgc
     4201 cgctgccgga cgtgcgcggt cgcgagcaga tcctgaaggt gcacatgcgc aaggtgccgg
     4261 tcggcggcga cgtgcgtccg agcctgatcg cccgcggcac gccgggcttc tccggtgccg
     4321 atctcgccaa tctggtcaac gaggccgcgc tgttcgccgc ccgcgccaac aagcgcgacg
     4381 tggacatgga cgatttcgag aaggccaagg acaagatcat gatgggcgcc gagcgcaagt
     4441 cgatggtgat gagcgaggac gaaaagaagc tgaccgccta tcacgaggcc ggccacgcca
     4501 tcgtcggccg gctggtgccg tcgcacgatc cggtttacaa ggtcagcatc atcccgcgcg
     4561 gccgggcgct gggcgtgacc atgttcctgc cggaaggcga ccgctacagc ttcagcaagc
     4621 agcgcttgga gagccagatt tccagcctgt tcggcggccg gatcgccgag tcgatcatct
     4681 ttggggcgga ttttgtcacc accggcgccc agaacgacat cgagcggtcc accgacatcg
     4741 cccgcaacat ggtgaccaag tggggcctgt ccgagcgctt ggggccgttg acttacagcg
     4801 aggacgacgg cgaagtgttc ctgggccgca gcgtgacccg gcacaagaac gtctcggacg
     4861 agaccgctca cgtcatcgac gaggaaattc gcacgatcat caaccgcaat tacctgcgct
     4921 ccgagcagat cctgcgcgaa aacctcgaca agctgcacgc catggccgat gcgctgatca
     4981 agttcgagac catcgactcc gagcagatcg acgacatcat ggccgggcga ccgccgcgcg
     5041 aaccggccga agcggacgcc gaccaagagc ctcccgcgag cggttcgccg gtcagcttgg
     5101 ataaggaccg ggcgggtgac gggaaaattg gcggtcccgc cccgcagcat tgaacgatgc
     5161 ggttggactg cgcgggcaag gctctcgatc tgagccggcc cgtcgtgatg ggggtgctga
     5221 atgtcacccc cgactccctt tccgacggcg gatattttct acgcttttcc gacgctctgc
     5281 gccgcgccga agccatggtg gccgaaggtg ctgccatc
//
LOCUS       DAOQJH010000079         5316 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_8985_length_5316_cov_4.428626, whole genome shotgun sequence.
ACCESSION   DAOQJH010000079 DAOQJH010000000
VERSION     DAOQJH010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 5316)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5316)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5316
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8985_length_5316_cov_4.428626"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..4220)
                     /locus_tag="PLQ18_02990"
     CDS             complement(<1..4220)
                     /locus_tag="PLQ18_02990"
                     /inference="COORDINATES: protein motif:HMM:NF013502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HNE00646.1"
                     /translation="MQESEDDPKAGADDPAAHVERDAERDETEDGADSGPRPPLIVGI
                     AASAGGLEALGGLLRPLPATGRLAYVVVQHLSPSHPSLLVQLLQRECALRVVDVSDGQ
                     EIVADTVYVTPPRTNVSIHDGRLVLTEASDPGVPKPSADHFLHALAEDQGENAIAVIL
                     SGTGTGGAEGVRAVKAAGGFTFAQDDASAKYSGMPAAAIATGCVDLVLPPERIAEELM
                     RLAQLGVQGGLPVGSPHVPASVFERMLRLVQRHTGTDFLNYKPNTLRRRVARRMLATH
                     TATLEDYEMLLATQSGEIERLYQDILISVTAFFRDVPAFEALAGVLREMVAEKSPDDD
                     LRVWVPGCATGEEAYTIAILLAEALGTRLGERKVHIFATDLDTNALAIARRGVYHHSV
                     ASVVPPPLLERYFTTAGESYQVGKLLRDLVIFARHDLAQDPPFLRLDLISCRNLLIYF
                     KSPLQERVLDMFHYALRPGGWLLLGKSENPHTADLFAPSAGKVKLFRRRDAARARSLQ
                     MPRVPVRENDRATRPPARAGMTLEERVRLVALAAYAPAGIVVDEAMEVRYTLGDVSPY
                     IRLAEGKANLSITTLIRRELHVDLRGLILKCRSSNALTTSRRITMDGGPAVRLAVHPL
                     AGDFPEDPLFLVCFEHPRAEIETGRAGAESEQMQRTLELEQELAASREHLQTVIEELE
                     TSNEELQSLNEEMLSANEELQSTNEELETANEELQSTNEELITVNEELQSRSAQLMAA
                     NADLENLKNTLPCPVLVVDARLRLMFANPAADRLFGLDPDDSVGPLHALRANLDFSGL
                     REVVERVIDAGNEMTHQFAGDRDHLLRARPYRDAQGRIRGAVLVFWDNTDIRRAEERI
                     REQVRQMALQSRALAAADNGIVICDARAPDRPISYVNAGFTRITGYSAEEVMGRNCRF
                     LQGRETDRGIITEIARALADQRQISVLLRNYRKDGEPFWNELNISPMRDEDGVCTHFI
                     GVQNDVTERVQAAERARLALAVFENTSEGIMITDAHLHLVAVNPAFTAITGYRSEDAI
                     GQRPTLIASGRHGQDFYRQMWRELQAHGEWHGELWNRRRSGEVYPELLHIHVVQDDAG
                     HVTHYVGIFSDITELKAAERRMHEIAYFDSLTGLPNRSLFSDRLARALLRSRRDNTRC
                     AVLFLDVDRFKNVNDTWGHETGDKLLQEIGTRISGCIRGSDTLARYGGDEFVLLAEDV
                     GDGRRLSELAERIHDALTGPVSIDGQTIDTGVSIGIAICPQDGTEAEVLLASADAAMY
                     QAKHAGRHTHRFHDPQMNALWIERMALEHELRAALGGSGLELHFLPLVRLADRSIVGC
                     EALLRWRHGQRGLLAPAEFLVSVADVKLLGDIDRWVLRSAAAQHHAWAGTPAGALPMA
                     VNLSGALLRDGERLPGLLAEIAGSWLSF"
     gene            4356..4991
                     /locus_tag="PLQ18_02995"
     CDS             4356..4991
                     /locus_tag="PLQ18_02995"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007192901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HNE00647.1"
                     /translation="MGERIEIHPVNPQPRLIRRAVEVLREGGVIAYPTDSCYALGCRI
                     GDKDAQTRIQRIRQIDDRRHNFTLICRDLSELSLYARVDNAQFRLLKALTPGPFTFIL
                     RATHEVPRRLQNEKRKTIGLRVPQHVIVAALLAELGEPIMSATLIPPGEDRAVSDPED
                     IETGLGRELDLIVDGGNCGIEPTTVLDLVEGLPPVLIRQGRGDASAFLAEA"
     gene            5069..>5316
                     /locus_tag="PLQ18_03000"
     CDS             5069..>5316
                     /locus_tag="PLQ18_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011330546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HNE00648.1"
                     /translation="MPELNDIQRLAIMALPILFAITLHEVAHGWAALRLGDTTAWQQG
                     RLTLNPLAHIDPIGTIIVPLVLYFTAGFIFGWAKPVPV"
BASE COUNT          749 a         1831 c         1890 g          846 t
ORIGIN      
        1 tcgaaggaca gccacgaacc ggcgatctcg gcgagcaggc ccggcagccg ctcgccgtcg
       61 cgcagcagcg cccccgagag gttcacggcc atcggcagcg cgccggccgg cgtgccggcc
      121 caggcgtggt gctgcgcggc ggcgctgcgc agcacccagc ggtcgatgtc gccgagcagc
      181 ttcacgtcgg cgaccgagac gaggaactcc gccggcgcca gcaggccgcg ctgcccgtgg
      241 cgccagcgca gcagcgcctc gcagccgacg atgctgcgat cggccaggcg caccagcggc
      301 aggaagtgca gctccagtcc gctgccgccg agtgccgcgc gcagctcgtg ttcgagcgcc
      361 atgcgctcga tccacagcgc gttcatctgc ggatcgtgga agcggtgcgt gtgccggccg
      421 gcgtgcttgg cctgatacat cgcggcatcg gcgctggcga gcaggacctc ggcttccgtg
      481 ccgtcctgcg gacagatggc gatgccgatc gacacgccgg tgtcgatcgt ctggccgtcg
      541 atcgacaccg ggccggtgag cgcatcgtgg atgcgctcgg ccagttcgct gagccggcgg
      601 ccgtcgccga catcctcggc gagcagcacg aactcgtcgc cgccgtagcg cgccagcgtg
      661 tcgctgccgc ggatgcagcc ggagatgcgc gtgccgatct cttgcagcag cttgtcgccg
      721 gtctcgtggc cccaggtgtc gttgacgttc ttgaagcggt cgacatcgag gaacagcacc
      781 gcgcagcgcg tgttgtcccg ccggctgcgc agcagcgccc gcgccagccg gtcgctgaac
      841 aggctgcggt tcggcagccc ggtcagcgag tcgaagtagg cgatctcgtg catgcgccgc
      901 tcggcggcct tcagctcggt gatgtcgctg aagatgccga cgtaatgcgt gacgtggccg
      961 gcatcgtcct gcacgacgtg gatgtgcagc agctccgggt acacctcgcc gctgcgccgg
     1021 cggttccaga gctcgccgtg ccactcgccg tgcgcctgca gctcgcgcca catctgccgg
     1081 tagaagtcct ggccgtgacg gccggaggcg atcagcgtcg ggcgctggcc gatggcgtcc
     1141 tcgctgcggt agccggtgat cgccgtgaac gccgggttga ccgccacgag gtgcaggtgc
     1201 gcgtcggtga tcatgatgcc ttcgctggtg ttctcgaaca ccgccagcgc cagacgcgcg
     1261 cgctcggccg cctgcacgcg ctcggtcacg tcgttctgca cgccgatgaa gtgcgtgcag
     1321 acgccgtcct cgtcgcgcat cggcgagatg ttcagctcgt tccagaacgg ctcgccgtcc
     1381 ttgcggtagt tgcgcagcag cacgctgatc tggcgctggt cggcgagcgc gcgcgcgatc
     1441 tcggtgatga tgccgcgatc ggtctcgcgc ccctgcagga agcggcagtt gcggcccatg
     1501 acctcttcgg ccgagtagcc ggtgatgcgc gtgaagccgg cgttgacgta gctgatcggg
     1561 cggtcgggtg cgcgcgcatc acagatgacg atgccgttgt cggccgccgc cagcgcccgc
     1621 gactgcagcg ccatctgccg cacctgctcg cggatgcgtt cctcggcgcg gcggatgtcg
     1681 gtgttgtccc agaacaccag caccgcgccg cggatgcggc cctgcgcgtc gcggtagggg
     1741 cgggcgcgca gcaggtgatc gcggtcgccg gcgaactggt gcgtcatctc gttgccggca
     1801 tcgatgacgc gctcgaccac ctcgcgcagg cccgagaagt cgaggttcgc gcgcagtgcg
     1861 tgcaacggcc cgaccgagtc gtcggggtcg aggccgaaca gccggtcggc ggcggggttg
     1921 gcgaacatca gccgcaggcg cgcatcgacg accagcaccg ggcagggcag cgtgttcttg
     1981 aggttttcga gatcggcgtt ggccgccatc agctgggcgc tgcggctctg cagctcctcg
     2041 ttgacggtga tcagctcctc gttggtcgac tgcagttcct cgttggcggt ctccagttcc
     2101 tcgttggtcg actgcagttc ctcgttggcc gagagcatct cctcgttgag cgactgcagc
     2161 tcctcgttcg aggtttcgag ctcctcgatg acggtctgca ggtgctcgcg cgaggcggcc
     2221 agctcctgtt cgagttcgag cgtgcgctgc atctgctcgg actcggcgcc ggcccggccg
     2281 gtctcgatct cggcgcgcgg atgctcgaag cagaccagga acagcgggtc ctcggggaag
     2341 tcgccggcca gcggatgcac ggccagacgc acggccgggc cgccgtccat cgtgatgcgg
     2401 cggctggtgg tcagcgcgtt gctgctgcgg cacttcagga tcaggccgcg cagatcgacg
     2461 tgcagctcgc gccggatcag cgtcgtgatc gacaggttgg ccttgccttc ggcaagacgg
     2521 atgtacggcg acacgtcgcc gagcgtgtag cgcacctcca tggcctcgtc gacgacgatg
     2581 ccggccggcg cgtaggcggc gagcgcgacc agccgcacgc gctcttcgag cgtcatgccg
     2641 gcgcgtgccg gcgggcgtgt ggcgcggtcg ttctcgcgca ccggcacgcg cggcatctgc
     2701 aggctgcggg cgcgggcggc gtcgcggcgg cggaacagct tgaccttgcc ggccgagggc
     2761 gcgaacagat cggcggtatg cgggttctcg gacttgccga gcagcagcca gccgccgggg
     2821 cgcagcgcgt agtggaacat gtcgagcacg cgctcctgca gcgggctctt gaagtagatc
     2881 agcaggttgc ggcagctgat caggtcgaga cgcaggaacg gcgggtcctg cgcgaggtcg
     2941 tggcgcgcga agatcaccag atcgcgcagc agcttgccga cctggtagct ctcgccggcg
     3001 gtcgtgaagt agcgctccag cagcggcggc ggcacgacgc tcgcgacgct gtggtggtag
     3061 acgccgcggc gcgcgatcgc gagcgcattg gtgtcgaggt cggtcgcgaa gatgtgcacc
     3121 ttgcgctcgc cgaggcgcgt gccgagggcc tcggcgagca ggatggcgat cgtgtacgcc
     3181 tcctcgccgg tcgcgcagcc cggcacccag acgcgcaggt catcgtcggg ggacttctcg
     3241 gcgaccatct cgcgcagcac gccggccagc gcctcgaagg ccggcacatc gcggaagaac
     3301 gccgtcaccg agatcaggat gtcctgatac agccgctcga tctcgcccga ctgggtggca
     3361 agcagcatct cgtagtcttc gagcgtcgcc gtgtgcgtcg cgagcatgcg tcgcgccacg
     3421 cgccggcgca gcgtgttcgg cttgtagttc aggaaatcgg tgccggtgtg gcgctgcacc
     3481 agcctgagca tgcgctcgaa caccgaggcc ggcacgtgcg gcgagccgac cggcagaccg
     3541 ccctggacgc cgagctgcgc caggcgcatc agctcctcgg cgatgcgctc gggcggcagc
     3601 accagatcga cgcagccggt ggcgatcgcc gccgccggca tgcccgagta cttggcgctg
     3661 gcatcgtcct gcgcgaacgt gaagccgccg gcggccttga ccgcgcgcac gccctcggcg
     3721 ccgccggtgc cggtgccgga gaggatcacg gcgatggcgt tctcgccctg gtcctcggcg
     3781 agcgcgtgca ggaagtgatc ggccgagggc ttgggcacgc ccgggtcgct cgcctcggtc
     3841 agcaccagcc ggccgtcatg gatcgagacg ttcgtgcgcg gcggcgtcac gtagacggta
     3901 tcggcgacga tctcctgccc gtcgctgaca tcgacgacgc gcagcgcgca ttcgcgctgc
     3961 agcagctgca ccagcaggct cgggtgcgac ggtgacaggt gctgcacgac cacatacgcc
     4021 agccgcccgg tcgccggcag cggccgcagc agcccgccga gggcttcgag cccgccggcc
     4081 gaggccgcga tgccgacgat cagcggcgga cgcggcccgg aatcggcgcc gtcttcggtc
     4141 tcgtcccgtt cggcgtcccg ttccacgtgc gcagccggat catcggcccc ggccttcggg
     4201 tcgtcttcgg attcctgcat ggctgaattc cggttgttct tcgctggatt ccgggcgctg
     4261 acgggctgct gacagaaacc ctgcggccgg tcatttgacc gcccgcgcgt ggcggtggca
     4321 aggtgcgcgc cgcttcttac agggtcggtc cgtccatggg cgagcgcatc gagatacatc
     4381 cggtcaatcc gcagccgcgc ctgatccggc gggcggtcga ggtgttgcgc gagggcggcg
     4441 tcatcgccta cccgaccgat tcctgctatg cgctcggctg tcgcatcggt gacaaggatg
     4501 cgcagacccg catccagcgt atccgccaga tcgatgatcg tcgtcataat ttcacgctga
     4561 tctgccggga cctgtcggag ctgagcctgt acgcgcgcgt cgacaacgcc cagttccggc
     4621 tgctgaaggc gctgacgccg gggccgttca cgttcatcct gcgcgccacg cacgaggtgc
     4681 cgcggcgcct gcagaacgag aagcgcaaga ccatcggcct gcgcgtgccg cagcacgtga
     4741 tcgtcgcggc gctgctcgcc gaactcggcg aaccgatcat gagcgcgacg ctgatcccgc
     4801 ccggcgagga ccgcgcggtc agcgatcccg aggacatcga gaccggcctc gggcgcgagc
     4861 tcgacctgat cgtcgatggc ggcaactgcg gcatcgaacc gacgacggtg ctcgatctgg
     4921 tcgagggcct gccgccggtg ctgatccgtc agggccgcgg cgacgccagc gccttcctcg
     4981 ccgaagcctg aagcggctgc acgggacggc atctgctgca gcgcaacgcc gttgtcagcg
     5041 tccggcctgc gccccgtata ctcgacccat gcccgaactc aacgacatcc agcgcctggc
     5101 catcatggcc ctgccgatcc tgttcgcgat caccctgcac gaggtcgccc acggctgggc
     5161 agcgctcagg ctcggtgaca ccaccgcctg gcagcagggc cggctgacgc tgaacccgct
     5221 cgcgcacatc gatccgatcg gcaccatcat cgtgccgctg gtgctgtact tcacggccgg
     5281 attcatcttc ggctgggcca aacccgtgcc ggtcag
//
LOCUS       DAOQJH010000080         5298 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_9033_length_5298_cov_3.750906, whole genome shotgun sequence.
ACCESSION   DAOQJH010000080 DAOQJH010000000
VERSION     DAOQJH010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 5298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5298
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9033_length_5298_cov_3.750906"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            175..1353
                     /locus_tag="PLQ18_03005"
     CDS             175..1353
                     /locus_tag="PLQ18_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009632925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HNE00649.1"
                     /translation="MDTPVPSGRAGRYMRQPTGYRAFIPAPLPPDPPLALHGELQSLL
                     SQADRALGRLDGSVLTLPNPDLFVFMYVRKEAVLSSQIEGTQSSLQDLLAAEAELFNE
                     HMPRDVDEVVNYVRAMRLGLTRLAELPVSVRLIREIHAELMQGVRGGRLTPGELRRSQ
                     NWIGPAGCTLATASFVPPPHEEVPRILGELEKFLHQEDELPPLVKIALAHVQFETIHP
                     FLDGNGRVGRLLITFLLTERGVLHKPVLYLSHYFRRHRQAYYEHLQAVRDRGEWERWL
                     AFFLRGVSEVAGEATETARRIQLLREQHRSAITDRLGRAAGNGHRVLESLFNRPIVTV
                     ADVQTLTGTTYAAANTLVARLAGLGVLAEMTGYARNRRFRYEPYVRLFTDEPLPEHPT
                     "
     gene            1701..3668
                     /locus_tag="PLQ18_03010"
     CDS             1701..3668
                     /locus_tag="PLQ18_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoX family phosphatase"
                     /protein_id="HNE00650.1"
                     /translation="MSADDEIVNPSHGPRFTDLLALAASRRQLLKLGAAGAALALFGR
                     SGAAADAPAAAAAPAAALAGLPGFTAIATSTEDTVRVPPGYEWAVLTRWGDPVGAQAG
                     QPRWRSDAGNDAAEQALQFGMHHDGMALFPLGTDGTRALLALNHEYVDHGLLFADGMD
                     GWSAAKVAKSMAAHGVSVVELVQADGRWSVVSPSQYARRITAATPMRLAGPAAGHALL
                     ATAADPQAQTVLGTFNNCACGQTPWGTYLSCEENFNGYFARTAGEPTALEKRYGIREK
                     SDYRWHEHEPRFDSALHPNEAHRFGWVVEIDPHDPAAVPVKRTALGRFKHENAAVTLA
                     ADGRVVVYLGDDQANEYLYRYVSKGRYTPGMARAAAGALLDDGILFVAHFGADGHGEW
                     RALVHGRDGLDAGNGFADQAEVLVKTRLAADVRGATKLDRTEWVAIHPGRAEVAVTLT
                     NNSGRKSADAADPRAPNPHGHILRWRPDGADHAADGFAWEVFVLAGDPQAEDASARGN
                     QPGLNAANTDAAFSSPDGLAFDARGLLWIETDVSTGKLWHPQLAPKQGAYQVFGNNQL
                     LVANPDTGEIRRFLTGPVGCELTGIAFSPDLRTLFVNIQHPGEPLDANTEFNDPTAPQ
                     RWSQWPDGPGGGRPRSATLAIRRTDGGPVGS"
     gene            complement(4027..4854)
                     /locus_tag="PLQ18_03015"
     CDS             complement(4027..4854)
                     /locus_tag="PLQ18_03015"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HNE00651.1"
                     /translation="MRERSFRSVWLSDLHLGNADCRALWLLDFLDACTCEQLYLVGDV
                     LDLWEMDRRGRWPATHGRVLAKLIALAAAGTRVVWLPGNHDEALRAHHGLRFGGVELL
                     PEAIHETADGRKLWVVHGDGFDDAARGGRLTTLIGDHAYDLALFLNRWSRRLRRLCGL
                     PYRSLALDLKTRIGPAARAIGRYERAAAHEAARREVDGIVCGHIHKPELRTIDGTLYA
                     NCGDWVESCTALVEHADGRLQVLHWADEARVTHVDVLDFPRAAAAVPAAAAMAGRLG"
BASE COUNT          867 a         1835 c         1865 g          731 t
ORIGIN      
        1 gcgcagaagg aaacgctgat ccgcctgatc atgaacatgc tggcgccggt ggaggccggg
       61 aaatgagttt gccgagcgca agccagcctt gatcaaggca cttcctcgtc gccatgctaa
      121 acgcagacta tcacatgcag tcatctgacc cgcgtgggca agcgaggaat gcacatggac
      181 accccggtgc cgtcgggccg cgcgggccgc tacatgcgtc agccgaccgg ttaccgtgcg
      241 ttcatccccg ccccgctgcc acccgaccca ccgcttgcgc tgcacggaga actgcaaagc
      301 ctgctgtccc aggccgatcg cgcgctcgga cggctggacg gctcggtgct cacgctgccg
      361 aaccccgatc tgttcgtctt catgtacgtg cgcaaggagg ccgtactttc gagtcagatc
      421 gaaggcacgc aaagctcgct gcaagacctg ctggcggccg aagccgaact gttcaacgag
      481 cacatgccgc gtgacgtcga cgaagtcgtg aactacgtac gcgccatgcg gctcggactg
      541 acacgactgg ccgagctgcc cgtatcggta cgactgattc gtgaaatcca tgccgaactc
      601 atgcagggcg tgcgcggcgg ccggctgaca cccggtgagc tgcgccgcag ccagaactgg
      661 atcggtccgg caggttgcac tctggcgacc gccagtttcg tgccgcctcc gcacgaggaa
      721 gtgccgcgaa tcctcgggga gctggaaaag ttcctgcatc aggaggatga gctgccgccg
      781 ctggtgaaga tcgcactggc gcatgtgcag tttgaaacca ttcacccttt cctcgacggc
      841 aacggccgcg tgggccggct gctgatcacc ttcctgctga ccgaacgcgg cgtgctgcac
      901 aaacccgtgc tctacctgtc gcactatttc cgccggcatc gacaggctta ctacgaacac
      961 ctgcaggcgg tgcgcgaccg tggcgaatgg gaaaggtggc tggctttctt cctgcgaggc
     1021 gtgagcgagg tggcagggga agcgaccgag acggcacgcc gcatccagct gttgcgggag
     1081 cagcatcgca gcgcgatcac tgaccggctt ggccgtgccg caggcaatgg acaccgggta
     1141 ctggaaagcc tgttcaaccg tcccatcgtg acggtggcag acgtgcagac actgactgga
     1201 accacgtatg cggcggccaa caccctggtg gcgcgtctgg ccggactggg cgtgctggcg
     1261 gaaatgacgg gttatgcacg caaccgcaga ttccgctacg agccctatgt ccggctgttc
     1321 acggatgagc cgcttccaga acacccgaca tgaacctgca ccgcgaacac ctcttcgcag
     1381 ccggaagctg acctcctcgt ccacggccgg cagtacgtcg aaggcgaggc ggcccgaaaa
     1441 gcgttcgcgc acttcgcaag aaccaccacg acccaggccc gagcctgcac cggcgcactt
     1501 cgcagtccgc cagcacggag aggctgcccg gatcattgcg gccgggaagt cctgctcgcc
     1561 cccgggacga tgatgtcgcg gctgcacgca cctgagcgcc gtggagtgaa gtgcactgca
     1621 aaccccccgt cacacagccc gcgcacactc gcacgccttc cgcggcccga gccggccgtt
     1681 tcgcgatcga ggtagtgccg atgtccgccg acgacgaaat cgtcaatccc agccacggcc
     1741 cgcggttcac cgatctgctg gcgctggccg ccagccgccg gcagctgctg aagctcggtg
     1801 ccgccggtgc ggcgctggcg ctgttcgggc gcagcggtgc cgctgccgat gcgccggcgg
     1861 ccgctgcggc cccggctgcc gcgctcgccg gcctgcccgg cttcacggcc attgccacca
     1921 gcaccgagga cacggtgcgc gtgccgccgg gttacgaatg ggcggtgctg acgcgctggg
     1981 gcgatccggt cggcgcgcag gccgggcagc cgcgctggcg cagcgatgcc ggcaacgatg
     2041 ccgccgagca ggcgctgcag ttcggcatgc atcacgacgg catggcgctg ttcccgctcg
     2101 gcacggacgg cacgcgcgcg ctgctggcgc tgaatcacga atacgtcgat cacggcctgc
     2161 tgttcgccga cggcatggac ggctggagcg ccgccaaggt ggcgaaatcg atggctgcgc
     2221 acggcgtcag cgtcgtcgag ctggtgcagg ccgatggccg ctggagcgtg gtctcgccgt
     2281 cgcagtacgc gcggcgcatc acggccgcga cgccgatgcg cctcgccggg ccggccgccg
     2341 ggcatgcgct gctggcgacc gccgccgatc cgcaggcgca gacggtgctc ggcaccttca
     2401 acaactgcgc ctgcggccag acgccgtggg gcacgtacct gagctgcgaa gagaacttca
     2461 acggctactt cgcgcgcacg gccggtgaac cgaccgcgct cgaaaagcgc tacggcatcc
     2521 gggagaagtc cgactaccgc tggcacgagc acgagccgcg cttcgactcc gcgctgcacc
     2581 cgaacgaggc gcaccgcttc ggctgggtgg tcgagatcga tccgcacgat ccggcggcgg
     2641 tgccggtcaa gcgcaccgcg ctcgggcgct tcaagcacga gaacgccgcg gtgacgctgg
     2701 ccgccgatgg ccgcgtcgtc gtctatctcg gcgacgatca ggccaatgaa tacctgtacc
     2761 gctacgtcag caagggccgt tacacgccgg gcatggcgcg cgccgcggcc ggggcgctgc
     2821 tcgatgacgg catcttgttc gtcgcgcact tcggcgccga tggtcacggc gagtggcggg
     2881 cgctggtgca tggacgcgac ggcctcgatg ccggcaacgg cttcgccgat caggccgagg
     2941 tgctggtgaa gacccggctc gcggccgatg tgcgcggcgc gacgaagctc gatcgcaccg
     3001 agtgggtcgc catccacccg ggccgcgccg aggtcgccgt gacgctgacc aacaacagcg
     3061 gccgcaagag cgccgatgcc gccgatccgc gcgcgccgaa cccgcacggc cacatcctgc
     3121 gctggcgccc ggatggcgcc gatcacgccg ccgacggctt cgcgtgggag gtgttcgtgc
     3181 tcgccggcga tccgcaggcc gaggatgcca gtgcccgcgg caatcagccc ggactcaacg
     3241 ccgccaacac cgatgccgcc ttcagctcgc cggacggcct cgccttcgat gcgcgcggcc
     3301 tgctgtggat cgagaccgat gtctcgaccg gcaagctctg gcatccgcag ctcgcaccga
     3361 agcagggcgc gtatcaggtc ttcggcaaca accagctgct ggtcgccaac cccgacaccg
     3421 gcgagatccg ccgcttcctg accggcccgg tcggctgcga gctgaccggc atcgccttca
     3481 gcccggattt gcgcacgctg ttcgtcaaca tccagcaccc cggcgagccg ctggacgcga
     3541 acaccgagtt caacgacccg accgcgccgc agcgctggag ccagtggccg gacggccccg
     3601 gcggcggccg gccgcgctcg gcgacgctgg cgatccggcg cacggatggc gggccggtcg
     3661 gcagctgagc gcgtgaagcg gacgtgtgcg cgaccggatg cgtcgcgcac acggttgcgg
     3721 acacgcagcc gcaaggcccg aagccgcacg tgcaaggctt tgcgggcgat ccgcaaggga
     3781 aaaagccttg cgcgcaaggc tggaagcgcg aagcgcaagg cccgaagcag cacgcgcaag
     3841 gctttgcgcg cgatccgcaa gggaaaaagc cttgcgcaca aggctggaag cgcgaagcgc
     3901 aaggctcgaa gccgtgcgtg caaggcttcg gacccgaagt gcaaggctcg aagccttgca
     3961 cctgcggcgg ccaggctttc ggctgcggga aaaagccgtg caggccagac gccgggccgg
     4021 ccggtttcag cccaggcggc ccgccatcgc cgcggctgcc gggacggcag cggccgcgcg
     4081 cgggaagtcg aggacatcga cgtgcgtgac gcgcgcctcg tcggcccagt gcagcacctg
     4141 cagccggcca tcggcgtgct cgaccagcgc cgtgcagctc tcgacccagt cgccgcagtt
     4201 ggcgtacagc gtgccgtcga tcgtgcgcag ctcgggcttg tggatgtggc cgcagacgat
     4261 gccgtcgacc tcgcggcgcg cggcctcgtg cgcggcggcg cgctcgtagc ggccgatggc
     4321 gcgggcggcc gggccgatgc gcgtcttcag atccagtgcc agcgagcggt acggcaggcc
     4381 gcagagccgg cgcagccgcc ggctccagcg gttcaggaac agcgcgagat cgtaggcgtg
     4441 atcgccgatc agcgtcgtca gccggccgcc gcgcgcggca tcgtcgaagc cgtcgccgtg
     4501 cacgacccat agctttcggc cgtcggcggt ctcgtggatc gcctccggca gcagctcgac
     4561 gccgccgaag cgcaggccgt gatgcgcccg cagggcctcg tcgtgattgc cgggcagcca
     4621 gaccacgcgc gtgccggcgg cagcgagcgc gatcagcttc gcgagcacgc gcccgtgcgt
     4681 ggccggccag cgcccgcggc ggtccatctc ccacagatcg agcacgtcgc cgaccagata
     4741 gagctgctcg caggtgcagg catcgaggaa atcgagcagc cagagcgcac ggcagtcggc
     4801 attgccgagg tgcaggtccg aaagccacac ggaccggaag gatcgctcgc gcatcatcgt
     4861 cattcgttca cccgctcgct ccggcccgga aggtcggctg agtctgggca gcggcgatga
     4921 agcggccgtg acgttcgcac ggcgaagcgg tggcgggatc gtgatgggcc ggtgacggcg
     4981 ggcgtggcgc tcagcggcag accgttggcc gggcgcgcga agggacacgc cgcccggccg
     5041 ggaccggagt tgccggtccg gccggacggc gcgggcaggg tcgggacggg gtggatgaca
     5101 tcggttcggg tgccgggcgg gagcggacag gccgcgcagg gttcagacgc cgtgcgcggc
     5161 gccgtgggca ccgaacaccg gcagggcgct gccgcggccg gtcgcggagt aaccaaccgg
     5221 acgctggccg tgcagggcct cgtggcgcag cgcgtcggcg cgggcgatgt accagtcgta
     5281 gtcgatcgtg ccgttggc
//
LOCUS       DAOQJH010000081         5222 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_9234_length_5222_cov_4.011806, whole genome shotgun sequence.
ACCESSION   DAOQJH010000081 DAOQJH010000000
VERSION     DAOQJH010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 5222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5222
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9234_length_5222_cov_4.011806"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..518)
                     /locus_tag="PLQ18_03020"
     CDS             complement(<1..518)
                     /locus_tag="PLQ18_03020"
                     /inference="COORDINATES: protein motif:HMM:NF018436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1206 domain-containing protein"
                     /protein_id="HNE00652.1"
                     /translation="MNEALHALETGARLGYLARAVVYFMIGTLALMAGLGYGGETEDP
                     KDALRDLFRQPFGEVLVIAAAAGLAGYGLWRLLMAVRDPEHALYADEGRAQRIGRRLG
                     YVGSGLANLSLAAFALSLQFPRLVPFGHGGSGDGTRDWTARLLRVPAGEWLVARVGVV
                     FLITAAVFAVRA"
     gene            752..2542
                     /locus_tag="PLQ18_03025"
     CDS             752..2542
                     /locus_tag="PLQ18_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019916312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HNE00653.1"
                     /translation="MHRILVLLSAFGLAAPALAGAVDDARLRDAAQESGNWLSYGRDY
                     GQQRFSPLTQINRETIGRLVPKWIYQTGTTASFQATPIVVDGVLYLSMPFDHVAAIDA
                     ASGRELWRYEHQRRTPKMCCGPASRGVAVAYGKVFIGTVDARLIALDAASGKPVWDVA
                     VATDEGPTESVGQLDAGKAATRPSATGSTGIGVNMAPLVVDGKVIVGITGVGYGLHLE
                     AETPNAPVGTVVGFAGRYGRPGFYAAFDAQTGRRVWQFDTVPASGWEGSFRSTTPDGV
                     ALPRDPAREQAELGKYPDAARYGGGSAWTTPAYDAVSGLLYVGIGNPSPQMDDRSRPG
                     DNLYTVSLAALDAGTGTLKWHFQQVPHDLWGYDVASPPLLFELPTDKGPVAAVGQASK
                     LGWFYAHERRSGALLFKSEAFVPQENLFAAPTAEGTRIAPGAVGGASWSPVAFDPASG
                     LAYVAAAHIPIRYTLREIAGTKGGEALRYTELKPVDEPNWGTLTAIATREGGRIRWQQ
                     RTDKPLIGGVLATAGGLVFTGEGDGFFDAFDAATGERLWRFQCGAGVNAPPVTYEVDG
                     VQYVAVAAGGNSLFGYKSGGALIVFALQRD"
     gene            complement(2688..3206)
                     /locus_tag="PLQ18_03030"
     CDS             complement(2688..3206)
                     /locus_tag="PLQ18_03030"
                     /inference="COORDINATES: protein motif:HMM:NF023696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3617 family protein"
                     /protein_id="HNE00654.1"
                     /translation="MPAVHRPALAVLLALVASGATAAPDLPKRKPGLWETTVSMPAMP
                     GGAMPAIKMCIDEKTDDLVGGPNGVGRENCEQVDIRREGARYVIESKCRFGETKTHTT
                     GWFEGSFDSDYKGELNTEYSPPMQGMKNGKVLIAGRWLGACPADMQPGDLLMPNGMKV
                     NPSKMPQGQPKP"
     gene            3487..4011
                     /locus_tag="PLQ18_03035"
     CDS             3487..4011
                     /locus_tag="PLQ18_03035"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HNE00655.1"
                     /translation="MKTTGITLLIGIGSEARGDDAAGLEVARLLSANPPPGWRIEQAT
                     GEATDLIMRWVGAERVIVVDAAQADWPAGTIVRLDGLRAVLPAQTRQTSTHATGLATA
                     VRLARALGLLPSELVLLAVCGECWCLGTGLSPAVAAATRHLAARLTGGEFPAAAHAWP
                     DRPADGPRPTGSGL"
     gene            4040..5137
                     /locus_tag="PLQ18_03040"
     CDS             4040..5137
                     /locus_tag="PLQ18_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018936502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNE00656.1"
                     /translation="MQRLSLAGLAVLLHVLWADRREIIGPRLRDGAITLGILHRLEDL
                     PVGWSDTAQEAAQYRVVPRADSARFGHVTGPLSWKRWLFLPEEVLFRAVRDDQDGGFT
                     LSATPTAPLRAFLGVRGCDLAAIAIQDRIFAHDPAYCARREATLIIAINCLEPGGTCF
                     CASMETGPQARGAFDLRLTEWLDAGTPSYLVESGSAAGRKLLARLPIEACTPEDQARV
                     EQALAQAATRMGRQLDTGGLAERLAARPGHRHWQQVAARCLGCANCTLVCPTCFCSTI
                     EDRTDLSGTTAERVRRWDSCFDLEHSYIVGGNVRADPAARYRQWLLHKLAYWHTQFGT
                     SGCTGCGRCITWCPVGIDLTVEAAAVAGSEA"
BASE COUNT          742 a         1801 c         1911 g          768 t
ORIGIN      
        1 caggcgcgca cggcgaagac cgccgcggtg atcaggaaca ccacgccgac ccgggccacc
       61 agccattcgc cggccggaac gcgcaacagc cgcgccgtcc agtcccgggt gccgtcgccg
      121 ctgccgccgt ggccaaacgg cacgaggcgc gggaactgca ggctcagcgc gaatgcggcc
      181 agcgacaggt tggcgaggcc gctgccgaca tagccgagcc ggcggccgat gcgctgcgcg
      241 cggccttcat cggcgtacag cgcatgttcg ggatcacgca ccgccatcag cagccgccag
      301 agtccgtaac ccgccagccc ggcggcggcg gcgatgacca gcacctcgcc gaacggctgg
      361 cggaacaggt cacgcagcgc atccttcgga tcctcggtct cgccgccgta gccgagcccg
      421 gccatcagcg ccagcgtgcc gatcatgaag tagacgacgg cacgggccag atagccgagg
      481 cgggcgccgg tttcgagggc atgcagggct tcgttcatcg ccggcggact ccgggcagtc
      541 ttgtcgttgt cgtcgtcggg ccgcagcggg gagcgcagcc ggtgcgcagc agcgcagtgc
      601 gctgcgggca ccggctgttt cgagccatgc cccggcacgc ggttcagtcg gaccggccgg
      661 ggtgcaaggg ctggcggcgc gtgccggcgc ctgcggctac agtgcccggc acgttccggg
      721 atgccccgat cccgccgcag agggcagtgc catgcatcga atccttgtgc tgctgtcggc
      781 attcggcctt gccgcgccgg cgctggccgg tgccgtcgac gatgcgcgcc tgcgcgatgc
      841 ggcgcaggag tccggcaact ggctctcgta tggccgcgac tacggtcagc agcgcttttc
      901 gccgctcacg cagatcaacc gcgagaccat cggccggctg gtgccgaagt ggatctacca
      961 gaccggcacg acggcctcgt tccaggccac gccgatcgtc gtcgacggcg tgctgtacct
     1021 gtcgatgccc ttcgatcacg ttgcggcgat cgacgccgcc agcggccgcg agctgtggcg
     1081 ctacgaacac cagcggcgca cgccgaagat gtgctgcggc ccggcgagtc gcggcgtggc
     1141 ggtggcctac ggcaaggtgt tcatcggcac cgtcgatgcg cggctgatcg cgctcgatgc
     1201 cgccagcggc aagccggtat gggatgtggc cgtcgccacc gacgagggac cgaccgagag
     1261 cgtcgggcag ctcgatgccg gcaaggctgc gacgcggccg tcggcaaccg gcagcaccgg
     1321 catcggcgtc aacatggcgc cgctggtggt ggacggcaag gtcatcgtcg gcatcaccgg
     1381 cgtcggctac ggcctgcacc tcgaagccga aaccccgaac gcgccggtcg gcaccgtcgt
     1441 cggcttcgcc ggccgctatg gccggccggg cttctatgcc gccttcgacg cgcagacggg
     1501 caggcgcgtc tggcagttcg acacggtgcc ggcgagcggc tgggaaggca gcttccgcag
     1561 cacgacgccg gacggggtgg cgctgccgcg cgatcccgcc cgcgagcagg ccgaactcgg
     1621 gaaatatccc gatgccgccc gctacggcgg cggttcggcg tggacgacgc cggcctacga
     1681 tgccgtgagc ggcctgctgt acgtcggcat cggcaacccg tcgccgcaga tggacgaccg
     1741 ctcccgcccc ggagacaacc tctatacggt atcgctcgcc gcactcgatg ccggcaccgg
     1801 cacgctgaag tggcacttcc agcaggtgcc gcatgatctc tggggctatg acgtggccag
     1861 cccgccgctg ctgttcgagc tgccgacgga caagggcccg gtggcggcgg tcgggcaggc
     1921 gtcgaagctc ggctggttct atgcgcatga gcgccgcagc ggggcgctgc tgttcaagtc
     1981 cgaggccttc gtgccgcagg agaatctctt tgccgcaccg acggcggaag gcacgcgcat
     2041 cgcgcccggt gcggtcggcg gtgcgagctg gtcgccggtg gctttcgatc cggcgagcgg
     2101 tctggcctac gtcgcggccg cgcacatccc gatccgctac acgctgcgcg agatcgccgg
     2161 caccaaaggc ggcgaggcgc tgcgctacac cgagctgaaa ccggtcgatg agccgaactg
     2221 gggcacgctg acggcgatcg cgacgcgcga gggcggccgc atccgctggc agcagcgcac
     2281 cgacaagccg ctgatcggcg gcgtgctcgc caccgccggc gggctggtgt tcaccggcga
     2341 gggcgacggg tttttcgatg ccttcgatgc cgccaccggc gagcggctgt ggcgcttcca
     2401 gtgcggggcc ggtgtcaacg cgccgccggt cacgtatgag gtcgacggcg tgcagtacgt
     2461 cgccgtcgcc gccggcggca attcgctgtt cggctacaag agcggcggcg cgctgatcgt
     2521 gttcgcgctc cagcgcgact gaaagtccgt cactcctcca tgtcgggtct gtctcctgct
     2581 ccctcgcgtt ttgccgcgtg aggaggcagg cgaggcccga cctgggcgct tgccggaccg
     2641 gggcctggcc ggcgctgcgg cacccgggcc ccgggcgtgc gccgggttca gggcttcggc
     2701 tggccctgcg gcattttcga cggattgacc ttcatgccgt tcggcatcag caggtcgccg
     2761 ggctgcatgt cggccggaca ggcgccgagc cagcgcccgg cgatcagcac cttgccgttc
     2821 ttcatgccct gcatcggcgg gctgtattcg gtgttcagct cgcccttgta gtcgctgtcg
     2881 aagctgccct cgaaccagcc ggtcgtgtgc gtcttcgtct cgccgaagcg gcacttcgac
     2941 tcgatcacgt agcgcgcgcc ttcgcggcgg atgtcgacct gctcgcagtt ctcgcggccg
     3001 acgccgttcg ggccgccgac cagatcgtcg gtcttctcgt cgatgcacat cttgatggcc
     3061 ggcatcgcgc cgccgggcat ggccggcatc gacacggtgg tctcccacag gccgggcttg
     3121 cgtttgggca gatcgggggc tgccgtggcg ccggaggcga cgagggcgag cagcacggcg
     3181 agggcagggc ggtgaacggc aggcatggcg gactccggag gacggggaca cgtgaggccg
     3241 cgctggcggc ggcagatacg accacgcagc agcgtagccg tcacgcggca cggacgaatc
     3301 ggacgcgcag cagcgctcgt gccctccgcc gtgcaatgcg caggcggcct cattgcggct
     3361 gccggcagcg cgaacccggg gagcatcttc catattcatc aggtagttgc gctgcatcaa
     3421 tgaagcggct ttcatgatcc gcagtgcatg gcgaggcgca tcggtcggcg ctagggttca
     3481 ggtgccatga agacgacggg aatcacgctc ctgatcggca tcggcagcga ggcccgcggc
     3541 gatgatgccg ccggtctcga agtcgcccgg ttgctcagcg cgaacccgcc gcccggatgg
     3601 cggatcgagc aggcgacggg agaagcgacc gacctgatca tgcgctgggt gggggccgag
     3661 cgggtgatcg tggtcgatgc cgcgcaggct gactggcctg cggggacaat cgtccggctc
     3721 gacgggctgc gtgcggtgct gccggcgcag acgcggcaga cctcgacaca cgcgacgggg
     3781 cttgccaccg ccgtgagact ggcccgggcg ctcggcctgc tgccgagcga actggtgctg
     3841 ctcgcggtgt gcggcgagtg ctggtgcctc ggcacgggtt tgagcccggc cgtggccgcg
     3901 gcgacgaggc atctggctgc gcgactcacc gggggggagt ttccggcggc agcgcacgca
     3961 tggcccgacc ggcccgcaga cgggccgcgg ccgacaggat ccggtctctg atccatccgc
     4021 aagcggaggg cgatccacca tgcagcgcct gagtctggcc ggccttgccg tgctgctgca
     4081 tgtgctgtgg gcggaccggc gcgagatcat cggtccgcgg ctgcgcgacg gtgcgatcac
     4141 gctcggcatc ctccatcgtc tcgaagacct gccggtcggc tggagcgaca ccgcccagga
     4201 agccgcgcag taccgcgtcg tgcccagggc cgacagcgcg cgcttcggtc atgtgaccgg
     4261 gccgctgtcg tggaagcgct ggctgttcct gcccgaggaa gtcctgttcc gggccgtgcg
     4321 cgacgaccag gacggcggct tcacgctgtc ggcgacgccg acggcgccgc tgcgggcgtt
     4381 tctcggcgtg cgcggctgcg atctggcggc gatcgcgatc caggaccgca tcttcgcgca
     4441 cgaccccgcg tactgcgcgc gccgggaggc gacgctgatc atcgccatca actgcctgga
     4501 gccgggcggc acctgcttct gtgcgtcgat ggagaccgga ccgcaggcgc gcggcgcctt
     4561 cgacctgagg ctgaccgaat ggctcgatgc cggcacgccc tcgtatctgg tcgagtccgg
     4621 cagtgcggcc ggtcgcaagc tgctcgcgcg gctgccgatc gaagcctgca cgccggaaga
     4681 tcaggcccgc gtcgagcagg cgctggcgca ggcagcgacg cgcatggggc ggcagctcga
     4741 taccggcggg ctcgccgaac gcctggccgc acggcccgga caccggcact ggcagcaggt
     4801 ggccgcgcgc tgcctgggct gcgcgaactg cacgctggtc tgtccgacct gcttctgctc
     4861 gacgatcgag gatcgcaccg acctgagcgg cacgacggcc gagcgcgtgc ggcgctggga
     4921 ttcgtgcttc gatctcgaac actcgtacat cgtcggcggc aacgtgcgcg ccgatcccgc
     4981 ggcccgctac cgccagtggc tgctgcacaa gctcgcgtac tggcacacgc agttcggcac
     5041 ttcgggctgc accggctgcg ggcgctgcat cacctggtgc ccggtcggca tcgacctgac
     5101 cgtggaagcg gcggcggtgg ccggcagtga agcatgaggc aggagacagg acagaccggc
     5161 gcgaatgatt ccccggcagg acgacggcag acgggcggga cgacccgtac cggctgccgc
     5221 tc
//
LOCUS       DAOQJH010000082         5206 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_9292_length_5206_cov_3.640846, whole genome shotgun sequence.
ACCESSION   DAOQJH010000082 DAOQJH010000000
VERSION     DAOQJH010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 5206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5206
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9292_length_5206_cov_3.640846"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="PLQ18_03045"
     CDS             complement(<1..204)
                     /locus_tag="PLQ18_03045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE00657.1"
                     /translation="MALEPRLVFDGAGAATAHAAGRDHADGADTGGSDAVGAADAHDT
                     NPSPDTQAAAGSPAANSSAPSIAL"
     gene            619..1533
                     /locus_tag="PLQ18_03050"
     CDS             619..1533
                     /locus_tag="PLQ18_03050"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNE00658.1"
                     /translation="MHTPDWDDYRYFLATAQTGSLSGAARELRTNQPTVGRRIAALEE
                     VLGVRLFHRHARGMSLTEAGARMLHSVQTMEEAALAAGRAVTGEDASLAGTVRISAPE
                     SIGSLFVAPRLGRLNGRYPAIQIVLQLTAHTADLLRNEADIAVRLFRPTAADLVARRV
                     GQVPFALYASPKYLNAAPALVAPADLAQHPQIGYDDEYVHLPEAEWQAGYARGARWSL
                     RSNSAFARAGGAAAGMGVALLPSVVGEIWPGLVRVLPNDAPEPREAWLVVHKDLRYVA
                     RIRVVLDFLAEIFEETRSPAAPQPARAV"
     gene            complement(1550..2293)
                     /locus_tag="PLQ18_03055"
     CDS             complement(1550..2293)
                     /locus_tag="PLQ18_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008216442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HNE00659.1"
                     /translation="MVYRWINLVQNRLLPAVCLLCGAPGEAPGRDLCAGCRRDLPLNA
                     HACPRCAEPLPASAPAGTVCGRCRRLPPPFAAAFAPYRYDWPLDRLIVELKFRRRLAV
                     ARVLGTLFAEALAVRLAAGAPRPQALVPVPLHPKRLAARGYNQAAELARPVARMLGLP
                     LLDAGRRVRDTAAQSALAFRERRRNVAGAFVLAQGVPHAHIALIDDVLTTGHTAGECA
                     RALQAGGVERVEVWTLARTPAPGKPGRTA"
     gene            2425..2823
                     /locus_tag="PLQ18_03060"
     CDS             2425..2823
                     /locus_tag="PLQ18_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018955112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE00660.1"
                     /translation="MKTENTPVGKVLRASESRRAAGMLNIGNIVAILLPVPLLIFWFG
                     ASMLVYAMNRHHPDKRVGHYTQQAAYRLYGVTGFFTAAATFIPGGGWTWYIVAWVAAA
                     LVLIPWSVLDLRRIRREDWADIILPEPRTA"
     gene            2820..3863
                     /gene="bioB"
                     /locus_tag="PLQ18_03065"
     CDS             2820..3863
                     /gene="bioB"
                     /locus_tag="PLQ18_03065"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HNE00661.1"
                     /translation="MNPTAAPLRHDWTVAEALELFALPFADLLYRAQGVHRQHFDPNA
                     VQVSTLLSIKTGACPEDCAYCPQSARHEGEVEREKLLAVEEVLSAARTAKASGASRFC
                     MGAAWRNPKDRDLDAVVAMVEGVKALGLETCVTLGMLRADQAQRLKDAGLDYYNHNLD
                     TSPEFYGSIITTRTYQDRLDTLGHVRSAGIKVCSGGILGMGESLRDRAGMLVQLANLP
                     EHPESVPINLLVQVEGTPLYGRDRLDGIDFVRTIAVARLLMPQSFVRLSAGRTDMSDE
                     LQALCFSAGANSIFYGEKLLTTANPDTDRDRALFAKLGVRALEIQETLPEAGDFVQEA
                     PCGCGAAKLRAGA"
     gene            3880..5043
                     /gene="bioF"
                     /locus_tag="PLQ18_03070"
     CDS             3880..5043
                     /gene="bioF"
                     /locus_tag="PLQ18_03070"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017365113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HNE00662.1"
                     /translation="MRDLAAELAALRAAGLYRAPRTVDSAQGPVIASGGREWLNFCSN
                     DYLGLAADARIAQALATAAARRGCGSGAAHLVCGHSAEHAALEEELAAFTGRPRALLF
                     STGYLANLGVLAALAGRGDSVLEDRLNHASLLDGGRLSGARLSRYAHADAEALEAKLA
                     VAGGERIVVTDGVFSMDGDVAPLPALADACRRHAAWLVVDDAHGFGALGPQGRGSLAL
                     HGLDAEAVPVVIGTLGKAFGTSGAFVAGSAELIEYLIQKARPYIYTTASSPALAAATR
                     ASLRIVQAEDWRRERLTALIARFRAGAAQLGFGARLLPSTTPIQPIVIGAAEDALAAA
                     QRLADRGICVTAIRPPTVPAGSARLRVTLSAAHTDAQLDRLLDALAAALPAPA"
     gene            5063..>5206
                     /locus_tag="PLQ18_03075"
     CDS             5063..>5206
                     /locus_tag="PLQ18_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004728468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-[acyl-carrier protein] methyl ester
                     esterase"
                     /protein_id="HNE00663.1"
                     /translation="MLYCQSWGSGPDLVLLHGWGMHGGVWDDTVEAFIDDWRVTVIDL
                     PGHG"
BASE COUNT          693 a         1917 c         1869 g          727 t
ORIGIN      
        1 cagcgcgatc gacggcgcgc tgctgttggc ggccggactg ccggccgcgg cctgggtgtc
       61 cggggagggg ttcgtgtcgt gtgcgtccgc cgcgccgacg gcatcgctgc cgccggtgtc
      121 tgcgccatcg gcgtgatcgc gcccggcggc gtgggccgtg gcggcgccgg cgccgtcgaa
      181 caccagccgc ggctccaggg ccatgcgcag cgaatgcggg cgcccgatgg ccgattcccg
      241 ggatcggaga ccttcctgtt ctggcgacac caaatcccga ggtccacgca tggctgtgcc
      301 tcccgcaccg gctgttccgc ctccagggat gcaggcacgc aaagcctggc catccatcga
      361 atccgtgagt ccatcggatc ggagggatgc cgcacgtgca tggtgggcgg taaggagcgg
      421 agtttgcttt ttcgtcactt gacgcgcaac cacgccttgc ctagtcgctt gcgtcaagaa
      481 atcgtcgcgt cggccgcggt cgcccgccgc gccggcgctg actacagtgg cgcgcatgag
      541 cggtccggat caccggtctc cagcaccaga agaacgccgc accggccccg gggggccgga
      601 atattccggg agagccccat gcatacgccc gactgggacg attaccgcta tttcctcgcc
      661 accgcgcaga ccggcagcct gtccggtgcc gcacgcgagc tgcgcaccaa ccagccgacc
      721 gtcgggcggc gcatcgcggc gctggaggaa gtgctcggcg tgcggctgtt ccatcgccac
      781 gcgcgcggca tgagcctgac cgaggccggc gcgcgcatgc tgcattcggt gcagacgatg
      841 gaagaagccg cgctcgccgc cggtcgcgcc gtgaccggcg aggatgcgag cctcgccggc
      901 accgtgcgca tctcggcgcc cgagagcatc ggcagcctgt tcgtcgcgcc gcgcctcggg
      961 cggctgaacg gccgctatcc ggcgatccag atcgtgctgc agctcacggc gcacaccgcc
     1021 gacctgctgc gcaacgaggc cgacatcgcc gtgcgcctgt tccgtccgac cgcggccgat
     1081 ctggtcgcac gccgcgtcgg tcaggtgccg ttcgcgctgt acgcctcgcc gaagtacctg
     1141 aacgccgcgc cggcgctggt ggcaccggcc gatctggccc agcacccgca gatcggctac
     1201 gacgacgaat acgtgcatct gcccgaagcc gagtggcagg ccggctacgc gcgcggcgcg
     1261 cgctggtcgc tgcgctcgaa cagcgccttc gcgcgcgccg gcggcgcggc ggccggcatg
     1321 ggcgtggcgc tgctgccgag cgtggtcggc gagatctggc cggggctggt gcgcgtgctg
     1381 ccgaacgacg cgcccgagcc gcgcgaggcc tggctggtcg tgcacaagga cctgcgctac
     1441 gtcgcgcgca tccgcgtcgt gctcgacttc ctcgccgaga tcttcgagga aacccgcagt
     1501 ccggccgcgc cgcagccggc acgggcggtc tgagccgggc gccctccgtt cacgccgtgc
     1561 gcccgggctt ccccggcgcc ggcgtgcgcg ccagcgtcca gacctcgacc cgctccacgc
     1621 cgccggcctg cagcgcccgt gcgcattcgc cggcggtgtg gccggtggtc agcacgtcgt
     1681 cgatcagcgc gatgtgcgcg tgcggcacgc cctgggccag cacgaaggcg ccggcgacgt
     1741 tgcgccggcg ttcgcgaaac gccagcgcgc tctgcgcggc ggtatcgcgc acgcgccggc
     1801 cggcatcgag gagcggcagg ccgagcatgc gcgccaccgg ccgcgccagt tcggccgcct
     1861 ggttgtagcc gcgcgccgcc agccgcttcg ggtgcagtgg caccggcacc agcgcctgcg
     1921 gacgcggcgc accggcggcg aggcgcacgg ccagcgcctc ggcgaacagc gtgccgagca
     1981 cgcgtgccac cgccagccgc cggcggaact tcagctcgac gatcagccgg tccagcggcc
     2041 agtcgtagcg gtacggggcg aaggcggccg cgaacggcgg cggcagccgg cggcagcggc
     2101 cgcagaccgt gccggccggc gcggacgccg gcagcggttc ggcgcagcgc ggacaggcat
     2161 gggcgttgag cggcagatcg cgccggcagc cggcgcagag atcgcgcccc ggcgcctcgc
     2221 ccggcgctcc gcagagcaga cagaccgctg gcagcagccg gttctgtaca agattgatcc
     2281 acctgtaaac catccggccg gctccgtggt tgacagccgg gcgccggctg ttagcatgcg
     2341 gcgcattccc gtccccgcgc gccgcgcacc ggtccggctg cgcccgaccg gtatccggct
     2401 gccgccccgc caggccgaaa ccggatgaag accgaaaaca cccccgtcgg aaaggtgctg
     2461 cgcgccagcg aatcgcggcg cgcggccggc atgctcaaca tcggcaacat cgtcgcgatc
     2521 ctgctgccgg tgccgctgct gatcttctgg ttcggcgcct cgatgctggt ctacgcgatg
     2581 aaccgccacc accccgacaa gcgcgtcggc cactacacgc agcaggccgc gtaccggctc
     2641 tacggcgtca ccggcttctt caccgccgcc gcgaccttca tcccgggtgg cggctggacc
     2701 tggtacatcg tcgcgtgggt ggccgcggcg ctggtgctga tcccgtggtc cgtgctcgat
     2761 ctgcgccgca tccgccgcga ggactgggcc gacatcatcc tgccggagcc ccgcactgca
     2821 tgaatccgac cgccgccccg ctgcgccacg actggaccgt cgccgaagcc ctcgaactgt
     2881 tcgcgctgcc gttcgcggat ctgctgtacc gcgcgcaggg cgtgcatcgc cagcacttcg
     2941 acccgaacgc cgtgcaggtc tcgacgctgc tgtcgatcaa gaccggtgcc tgccccgagg
     3001 actgcgccta ctgcccgcag agtgcgcgcc acgagggcga ggtcgagcgc gagaagctgc
     3061 tggcggtcga ggaagtcctg tcggcggcgc gcaccgcgaa ggccagcggt gccagccgct
     3121 tctgcatggg cgcggcctgg cgcaatccga aggaccgcga tctcgatgcg gtcgtggcga
     3181 tggtcgaggg cgtcaaggcg ctcgggctcg aaacctgcgt gacgctcggc atgctgcgcg
     3241 ccgatcaggc gcagcgcctg aaggacgccg gcctcgacta ctacaaccac aacctcgaca
     3301 cctcgccgga gttctacggc tcgatcatca cgacgcgcac ctatcaggac cgcctcgaca
     3361 cgctcgggca cgtgcgctcg gccggcatca aggtctgctc gggcggcatc ctcggcatgg
     3421 gcgaatcgct gcgcgatcgc gccggcatgc tggtgcagct tgccaacctc cccgagcacc
     3481 cggagagcgt gccgatcaac ctgctcgtgc aggtcgaggg cacgccgctg tacggccgcg
     3541 acaggctcga cggcatcgac ttcgtgcgca cgatcgcggt cgcgcggctg ctgatgccgc
     3601 agtccttcgt gcgcctgtcg gccggtcgca ccgacatgag cgacgaactg caggcgctgt
     3661 gcttctcggc cggcgccaac tcgatcttct acggcgagaa gctgctgacc accgccaacc
     3721 cggacaccga ccgcgaccgc gcgctgttcg cgaagctcgg cgtgcgcgcg ctcgaaatcc
     3781 aggaaaccct gcccgaggcc ggcgacttcg tgcaggaagc gccgtgcggc tgcggcgcgg
     3841 cgaagctgcg cgccggggcc tgagccctgc cgccggccga tgcgtgacct cgccgccgaa
     3901 ctcgccgcgc tgcgcgccgc cgggctgtac cgggcgccgc gcacggtcga ttccgcgcag
     3961 gggccggtga tcgcctccgg cgggcgcgag tggctgaact tctgcagcaa cgattatctc
     4021 ggcctcgccg ccgatgcccg catcgcgcag gcactggcca cggccgcggc gcgccggggc
     4081 tgcggcagcg gcgcggcgca tctggtctgc gggcattcgg ccgagcatgc ggcgctggag
     4141 gaggaactcg ccgccttcac cggccgcccg cgcgcgctgc tgttctcgac cggctatctc
     4201 gccaacctcg gcgtgctcgc ggcgctggcc gggcgtggcg acagcgtgct cgaagaccgg
     4261 ctgaaccacg cctcgctgct cgacggcggg cggctctccg gtgcgcggct gagccgctac
     4321 gcgcacgccg acgcagaggc gctcgaagcg aagctcgccg tggccggcgg cgagcgcatc
     4381 gtcgtcaccg acggtgtgtt cagcatggat ggcgatgtcg cgccgctgcc ggcgctcgcc
     4441 gatgcctgcc gccggcatgc ggcctggctg gtggtcgatg atgcgcacgg cttcggcgcg
     4501 ctcgggccgc aggggcgcgg cagtctggcg ctgcacgggc tggatgccga ggcggtgccg
     4561 gtcgtgatcg gcacgctcgg caaggctttc ggcaccagtg gtgccttcgt tgccggcagc
     4621 gccgagctga tcgaatacct gatccagaag gcgcgaccct acatttatac gacggcgtcc
     4681 tcgccggcgc tggcggcggc gacgcgcgcc agtctcagga tcgtgcaggc cgaggactgg
     4741 cggcgcgaac ggctgacggc gctgatcgcg cgcttccggg ccggcgccgc gcagctcggt
     4801 ttcggcgcgc ggctgctgcc ctcgacgacc ccgatccagc cgatcgtcat cggggccgcc
     4861 gaagatgcgc tcgccgctgc gcagcgcctc gccgatcgcg gcatctgtgt cacggcgatc
     4921 cggccgccga ccgtgccggc cggctcggcg cggctgcgcg tgacgctgag cgccgcgcac
     4981 accgatgcgc agctcgatcg cctgctcgac gcgctggccg cggcgctgcc ggcgccggcc
     5041 tgagtcgcgg aggccgcagg cgatgctgta ctgccaaagc tggggttcgg ggccggatct
     5101 ggtgctgctg cacggctggg gcatgcacgg cggcgtctgg gacgacacgg tcgaggcctt
     5161 catcgatgac tggcgcgtga ccgtcatcga cctgcccggt cacggc
//
LOCUS       DAOQJH010000083         5133 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_9496_length_5133_cov_4.508665, whole genome shotgun sequence.
ACCESSION   DAOQJH010000083 DAOQJH010000000
VERSION     DAOQJH010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 5133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5133
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9496_length_5133_cov_4.508665"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..365)
                     /locus_tag="PLQ18_03080"
     CDS             complement(<1..365)
                     /locus_tag="PLQ18_03080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE00664.1"
                     /translation="MNPDIHEFIHPHHLAVFMAAAREFNCHILIRKTGRASIEWVGKS
                     GYTGKRGDLKAKTANLEVAGHAVAGLVCSPLLQPLAFTEDRLASARKEWMKCSHLITE
                     PANGFDDDRPPQGCPTPYIL"
     gene            complement(436..3714)
                     /locus_tag="PLQ18_03085"
     CDS             complement(436..3714)
                     /locus_tag="PLQ18_03085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE00665.1"
                     /translation="MSDPVTSAAVAAAKFVGEKAISYLAMKVYEGSPCASPEQLTAIP
                     KLFCMAVRGAAGVDEKEYRQHLTQQIDLIAERLGRVEQAVSSINQNVTRLLQEMNLVH
                     LGIAEALVGQSAFSALQQIRSLYRNYSGRIASAGSGADELTVFFKDILQTSRLHLQTA
                     VIVDALVNPFAGKPGLLQNVLGQIAIKGSGKGLAACYELYETYTRALVLDLLKGQQLV
                     EAAIGYLQILAEERKLPELKAGLPYSAAGWQAECERQRQSVADAFHLQLDGFVLKRDL
                     DKASGLNPYFLSSEARGVFMQADLFSARLCQRYGIHGRIFSMGDRFDGTLKLDADTFP
                     ARARATIPLDEPLDYWSATTPGVYDCVAFANAFVVHRYRIPLTQAGTRKITSALPYSP
                     ADIETVDLGQAATGWFEETPAAGSAPAIFGSFIEMARAGGSFAFLSGEWEQGRRDQDS
                     SNPRTDGNDSYPQGVAQVAELHRMEVTSLREDRRMLGSVRQPEVGLSKGGKVIHALSA
                     RGDPDFHPELPEGRDQSHWRGAYATWWQTRKTITLPMPPEGQTLELHAALTPQLQADS
                     ALADAATLNGWLKGSWGRPLQPSSSISDAELLGKPVIVGVNYDPSLAFPDQGCSLTAG
                     ISLYPVFAEGPASHKDSPHTGTDDHAIALGNASCLPKYRGVFREQASTQPRQLRAFLS
                     DLYQPYGTRLKFRFEAYYRFAIETSGLDDTPFAMYARGRLENACFRLAPAGNALPPGI
                     YFDLKDPADLGFAYDYDGTGRPDHLVLYRPGSGRILISRHLGSNEFRPVGPRGPEGIG
                     IGGFDLKVAGDRIFPFDHAGSGHADHLLAYRPGNGAVSILKRNGGVFEAVFSEPVGGK
                     GIGGFNLDNPADQGFAFDYDSSGKLDHLVFYRPGQGALFIIRHDDKGFSPVYAQGAPG
                     KGIGGFDLSGAQDKVFAYDHDGRGRMDHLVLYRPGAGRISILKRQGSSFVAVYQRDSG
                     GIGGYDLANPADLGFAFDFDGKGSNDHLVFYRPGKGAIFILKPSDGDFKAVYRQGDGG
                     GGGGGGGGIGQYDLRHPADRVFPFDYRGNGLADHLVLYRPGTGVLQIIRNNSGAFVRI
                     YAKP"
     gene            complement(4205..4624)
                     /locus_tag="PLQ18_03090"
     CDS             complement(4205..4624)
                     /locus_tag="PLQ18_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020564579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HNE00666.1"
                     /translation="MADDVVLNKAASIERCVARAREEYAANPAGFAHDYTRQDAAILN
                     IQRACEAALDMGQHVIRRERLGIPQSARDVFTLLARAGWIDAGLAESLRRMVGFRNIA
                     VHEYQTLQMPVTIAIITQHLDDFLDYSRRMLQRDANG"
     gene            complement(4614..5009)
                     /locus_tag="PLQ18_03095"
     CDS             complement(4614..5009)
                     /locus_tag="PLQ18_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013820785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HNE00667.1"
                     /translation="MDPDTLLHRVRERLPALLAFYAFGSRIDGTAGPDSDLDLAVLVA
                     GYADPLELWTLSGELADLAGCPVDLLDLRAASTVMQYRILTTGRCCWARDASVAIYEA
                     GILSDKTELDTARAGLLADIVERGSIHGR"
BASE COUNT          879 a         1609 c         1782 g          863 t
ORIGIN      
        1 aggatgtaag gggtcgggca gccctgcggc ggtcgatcgt catcgaagcc gttggcgggt
       61 tcggtgatca ggtgactgca cttcatccat tccttgcgcg ccgacgccag acgatcttcg
      121 gtgaacgcca ggggttgcag caggggggaa cacacgagcc cggccactgc atgaccggcg
      181 acctccagat tggccgtctt ggccttcaga tcgccgcgct tgccggtgta gccggacttg
      241 ccgacccatt cgatgctggc ccgcccggtc ttgcggatca ggatgtggca attgaactcc
      301 cgcgcggcgg ccatgaacac cgcgaggtga tgcggatgga tgaattcatg gatgtcggga
      361 ttcatgaaag gctccgcgat aagggttcgt aaccggaatc cgccgccggc gtcctccctg
      421 acgccggcgg catgttcagg gcttggcgta aatccgcacg aaggcaccgc tgttgttgcg
      481 gatgatctgc agcacgccgg tgccggggcg gtacagcacc agatgatcgg ccagaccgtt
      541 gccgcggtag tcgaacggaa acacccggtc ggccggatgg cggagatcgt attgcccgat
      601 gccgccaccg ccgccgccgc cgccaccacc atcgccctgc cggtagaccg ccttgaagtc
      661 accgtccgac ggcttcagga tgaagatggc gccttttccg gggcgataga acaccaggtg
      721 atcgttgctg cccttgccgt cgaaatcgaa ggcaaagccc agatcggccg ggttggcaag
      781 atcgtaaccg ccgatgccgc cgctgtcgcg ctggtagacg gccacgaaac tgctgccctg
      841 acgtttcagg atgctgatcc tgccggcccc cgggcgatac agcaccagat gatccatgcg
      901 gcccctgccg tcgtggtcat acgcaaacac cttgtcctgc gcgccggaaa gatcaaagcc
      961 gccgatgcct ttcccgggtg caccctgcgc atataccggg ctgaaaccct tgtcatcgtg
     1021 cctgatgatg aacagcgcac cttggccggg ccggtagaac accagatggt cgagtttgcc
     1081 gctgctgtcg taatcgaagg caaaaccctg atcggcggga ttgtcgagat tgaagccgcc
     1141 gatgcccttg ccgccgacgg gttcggaaaa caccgcctcg aacacgccgc cgttgcgctt
     1201 gaggatgctg acggcgccat tgccgggccg gtaggcgagc aggtgatcgg catggccgct
     1261 gccggcatga tcgaacggga agatccggtc gcccgccacc ttcagatcga agccgccgat
     1321 gccgatccct tcggggccgc gcggaccgac gggccggaat tcgtttgaac cgagatggcg
     1381 gctgatcagg atcctgccgc tgccggggcg gtacaggacc agatgatccg gccggccggt
     1441 gccgtcgtag tcgtaggcaa agccgagatc ggcagggtct ttcaggtcga aataaatgcc
     1501 gggcggcagc gcattgccgg cgggggccag ccggaagcag gcattttcca gccggccgcg
     1561 cgcatacatc gcaaacggcg tgtcatcgag accggaggtt tcgatcgcga aacggtaata
     1621 agcttcgaag cggaatttca gccgcgtgcc gtatggctgg tacagatcgc tcaggaaggc
     1681 acgcaactgc cggggctgcg tcgaggcctg ctcgcggaac acgccccggt acttcggcag
     1741 gcaactggca ttgccgagcg caatcgcatg gtcgtccgtg ccggtgtgcg gggaatcctt
     1801 gtggctggcc gggccttcgg cgaataccgg atacaggctg atgccggcgg tcagcgagca
     1861 gccctgatcc ggaaacgcca gcgacggatc gtaattgacg ccgacgatga ccggcttgcc
     1921 gagcagttcc gcatcgctga tcgaagagga aggctgcagc ggccggcccc agctgccctt
     1981 gagccagccg ttgagcgtcg cggcatcggc gagggcgctg tcggcctgca gttgcggggt
     2041 cagcgcggcg tgcagttcga gggtctggcc ctcgggcggc atcggcagcg tgatggtctt
     2101 gcgcgtctgc caccaggtcg catacgcccc gcgccagtgg ctctgatcgc ggccctcggg
     2161 cagctccggg tggaaatccg gatcgccgcg ggcactgagg gcgtgaatca ccttgccgcc
     2221 cttggaaagc cccacttccg gctgacgcac gctgccgagc atgcgccggt cctcgcgcag
     2281 gctcgtgact tccatgcggt gcagctcggc cacctgggcg acgccctgcg gatagctgtc
     2341 attgccgtcg gtgcgcgggt tgctgctgtc ctgatcgcgg cggccctgct cccactcgcc
     2401 gctcaggaac gcgaagctgc cgccggcgcg ggccatctcg atgaagctgc cgaagatcgc
     2461 cggcgcggaa ccggccgcgg gggtttcctc gaaccagccg gtggcggcct gcccgagatc
     2521 gacggtttcg atgtcggccg gggaataggg cagcgcgctg gtgatcttgc gcgtgccggc
     2581 ctgcgtgagc gggatgcggt agcggtggac gacgaaggca ttggcaaacg ccacgcagtc
     2641 gtagacgccg ggcgtcgtgg cgctccagta atccagcggt tcatcgagcg ggatcgtggc
     2701 gcgggcgcgg gcggggaagg tatcggcgtc gagcttcagc gtgccgtcga accgatcgcc
     2761 catgctgaag atgcgcccgt gaatgccgta gcgctggcag aggcgggcgc tgaagagatc
     2821 ggcctgcatg aacacgccgc gggcttcgga cgacaggaag tacggattga gcccgctggc
     2881 cttgtccaga tcgcgtttca gcacgaaacc gtcgagttgc aggtgaaagg catcggctac
     2941 ggactggcgc tggcgctcgc attccgcctg ccagccggcg gcgctgtacg gcaggccggc
     3001 cttgagttcg ggcagctttc tttcctcggc gaggatctgc agataaccga tggccgcctc
     3061 caccagttgc tgacccttga gcagatcgag caccagtgcg cgggtatagg tttcgtacag
     3121 ctcataacag gccgccagtc ccttgccgct gcccttgatg gcaatctgcc cgagcacgtt
     3181 ctgcagcagc ccgggcttgc cggcgaacgg attgaccagc gcatcgacga tgacggcggt
     3241 ctgcagatgc aggcggctgg tctgcaggat gtccttgaag aacaccgtca gttcgtccgc
     3301 gccgctgccg gcactggcga tgcgtccgct gtaattgcgg tacaggctgc gaatctgctg
     3361 cagcgccgaa aaggccgatt gcccgaccag cgcctcggca atccccagat gcacgaggtt
     3421 catttcctgc agcagccggg tgacattctg gttgatgctg ctcacggcct gttcgacgcg
     3481 cccgaggcgc tcggcaatca ggtcgatctg ctgcgtcagg tgctggcggt attccttctc
     3541 gtcgacgccg gcggcgccgc ggaccgccat gcagaacagt ttgggaatcg cagtcagttg
     3601 ttcggggctg gcgcagggcg agccttcgta gaccttcatg gccaggtaac tgatggcttt
     3661 ctcgccaacg aacttggcag cggcaacggc cgccgaagtg accggatcgg acatgggcgg
     3721 atccctctgg gcagccgtgg aaggatgggt ctggacagtc atgagcgggc gactcggttc
     3781 cggatcggaa aggaacgtga ccagccggat cgccggggcg gcagaccgca ccggaatccg
     3841 caaagccgtt cgcccggctg cccgtcgcag acgcacacct gcgcctgtcc atgcaccgcg
     3901 ggcgctggcg gtgcaccgga atccggtggg catgaaaaag gctggggaga aacccgggtg
     3961 tcatccggaa tcggcagggc cgatggctgg ggaccggcac ggggggattg tccggtcggc
     4021 agcgaactgc ggtgtgcagg aggaagggga ggaaggcagg ggatgacggt ctgccctgac
     4081 aaaccgggca atcacggacc gcccggttca tgccgcagca agctagtaga cggggactga
     4141 ctgtgcaatg cagcgggcgg gcgcgagagg gcaatcaggt ggcatcggtc agtccgtgca
     4201 ccttttatcc attcgcatcg cgttgcagca tccggcggct gtaatcgaga aagtcatcga
     4261 gatgctgcgt gatgatggca atcgtcaccg gcatctgcag cgtctggtat tcatgcacgg
     4321 cgatgttgcg aaagccgacc atgcgcctga gcgattccgc cagccccgca tcgatccagc
     4381 cggcacgggc cagcagcgtg aacacgtcgc gggcgctttg cggaatgccg agtcgttcgc
     4441 ggcggatcac atgctggccc atgtccagcg ccgcctcgca ggctcgctgg atgttcagga
     4501 tcgcagcgtc ctggcgcgtg tagtcatggg cgaagccggc cgggttggcg gcgtattcct
     4561 cgcgggcgcg cgccacgcag cgctcgatgc tggcggcctt gttcagaacc acgtcatcgg
     4621 ccatggatgc tgccccgttc gacgatatcg gccagcagcc cggcgcgggc cgtgtcgagt
     4681 tcggtcttgt cactgaggat gccggcctcg tagatcgcca ccgaggcgtc gcgcgcccag
     4741 cagcagcggc cggtggtgag gatgcggtac tgcatcaccg tcgaggcggc gcgcaggtcg
     4801 agcagatcga ccgggcagcc ggccaggtcg gccagctcgc cggacaaggt ccacagttcg
     4861 agcggatcgg cgtaaccggc gaccagcacg gccagatcca gatcgctgtc cgggccggcc
     4921 gtgccgtcga tgcggctgcc gaaggcatag aacgccagca gcgccggcag acgctcgcgc
     4981 acgcggtgca gcagcgtgtc cgggtccatg cgcaggaggt ccatgcgggg gccggcgccg
     5041 cttcagcccc agaccgtcgc cagcgcccgg cgcacgaact ccggctgcgc cagcgccgcg
     5101 cggtgcatgt cggcgttgta gtagtcgcag gcg
//
LOCUS       DAOQJH010000084         5115 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_9549_length_5115_cov_4.150395, whole genome shotgun sequence.
ACCESSION   DAOQJH010000084 DAOQJH010000000
VERSION     DAOQJH010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 5115)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5115)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5115
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9549_length_5115_cov_4.150395"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..885
                     /locus_tag="PLQ18_03100"
     CDS             <1..885
                     /locus_tag="PLQ18_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002685594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNE00668.1"
                     /translation="VIFGQDQAIEALGSAIKMARAGLGTERKPVGSFLFAGPTGVGKT
                     EVTRQLALALGVEFLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTEAVTKHPHSV
                     LLLDEIEKAHPDVFNLLLQVMDHGTLTDNNGRKADFRNVILVMTTNAGADQIERSGIG
                     FTTEDHSTDSLEVIKRMFSPEFRNRLDAIIQFKALESDTIGHVVDKFIIELEAQLVAK
                     HVTLEVSGAARAWLAVRGYDRKMGARPMARVIQEHIKKPLAEELLFGRLASGGHVLID
                     ADGDGLVIDFAEATAEVS"
     gene            complement(952..1170)
                     /gene="infA"
                     /locus_tag="PLQ18_03105"
     CDS             complement(952..1170)
                     /gene="infA"
                     /locus_tag="PLQ18_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002553999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HNE00669.1"
                     /translation="MSKEDHIEMEGTVIETLPNTMFRVQLENGHVVTAHISGRMRKHY
                     IRILTGDKVRVELTPYDLSKGRITYRGR"
     gene            complement(1335..2069)
                     /locus_tag="PLQ18_03110"
     CDS             complement(1335..2069)
                     /locus_tag="PLQ18_03110"
                     /EC_number="2.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008737659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004057 - arginyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0016598 - protein arginylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginyltransferase"
                     /protein_id="HNE00670.1"
                     /translation="MAAPRSELGSLRLFLTDAHPCSYLAGHTARNLVADPLAVCDTLY
                     SALVLHGFRRSGEHVYRPHCQGCHACQSLRIPVRQFQPNRSQRRTQARNLDLQVSLVE
                     PVLREEHFQLFARYLALRHPDGGMDDATPDSYLGFIRSSWSRTFLAEFRDADGRLLAV
                     AVVDVLGDGLSAVYTYYDPAAEARAPGTAAILWQIEEARRRGLAYVYLGYWIAGCRKM
                     NYKTRFRPCEVFINGDWRSPPETPVR"
     gene            complement(2072..2788)
                     /gene="aat"
                     /locus_tag="PLQ18_03115"
     CDS             complement(2072..2788)
                     /gene="aat"
                     /locus_tag="PLQ18_03115"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013332814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HNE00671.1"
                     /translation="MIAWLDARNPNQPFPPAEAALVEPNGLLAAGGSLAPARLLNGYR
                     QGIFPWYGKGQPILWWSPDPRTIFLPDRIRVSHSLRKVLRSGRYTVTVDHAFARVIAE
                     CAAPRPEAPESWITREMREAYCRLHVLGYAHSIETWHGERLVGGLYGVALGGVFFGES
                     MFSRMNDASKVALVHLGRLLVQRGFGFIDCQLETGHLLSLGAQSLPRAEFLKLLHEHV
                     HRLLPATPWTLPAEAAPGAG"
     gene            complement(2785..3933)
                     /locus_tag="PLQ18_03120"
     CDS             complement(2785..3933)
                     /locus_tag="PLQ18_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009148347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNE00672.1"
                     /translation="MHLRCHESFRELPAAEWNTLVRDEDPFLQHAFLVALENHGVTGP
                     GTHWQPRPLSVRDDDGRLLGGCPLFLKTDSWGEFVFDWGWADAYHRNGLRYYPKLVAA
                     IPHTPATGQRLLVAAGADAGAVMALLAEGARELAAGLGCSTLHVLFPQAGELESLTAA
                     GLTHRLGCQFHWQDRGYRDFDDFLDAFSAKKRKNLRQERRRVREAGLELERLPGNAVS
                     EAQWRVFHRFYRNTFEQRGCPAPMSLPFFLEIGRTLGERVLLVLARRGHDIVAAAISY
                     AGARTLYGRHWGCAESFDALHFEACYYQGIEHCIEHGLTRFEPGAQGEHKVARGFEPV
                     LTHSAHWVAHPGFRQAIDAFLHRERPAIVEYARELTARLPYRSGEAAA"
     gene            4208..>5115
                     /locus_tag="PLQ18_03125"
     CDS             4208..>5115
                     /locus_tag="PLQ18_03125"
                     /inference="COORDINATES: protein motif:HMM:NF024882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HNE00673.1"
                     /translation="MTPAGVKIVRHISHGLREIGFLLLATVAAFLFIALASYVPADPA
                     WTHTGTSAPMNLGGVAGAWFADIALYFFGYPAYLFPLGLVFAGWRLFKRNGLAEIDAE
                     IIVFRTVGFLVTVGTGCGLAALHLQPGALRGSAGGIVGDLCGHGLINAFGPGGGTLFL
                     AALFLAGVTLASGLSWLSLVDLIGKSIWDVAALFARAGSWVAERIAPRRDEAGDDEPL
                     QQGRAPELSLAGGRQEPVLVAGLPPQAPPVPVSKPVTEPEKPATGSDGPAEPATAGKR
                     GRIEPSFDATTAPWDLGEDDEPKPPLP"
BASE COUNT          787 a         1689 c         1846 g          793 t
ORIGIN      
        1 gtgatcttcg gccaggacca ggcgatcgag gccctgggca gcgcgatcaa gatggcgcgc
       61 gcgggtctcg gtaccgagcg caagcccgtg ggatccttcc tgttcgcggg tccgaccggc
      121 gtgggcaaga ccgaggtcac gcgccagctc gcgctcgccc tcggcgtcga gttcctgcgt
      181 ttcgacatgt ccgagtacat ggagcgtcac acggtctcgc gcctcatcgg cgcgccgccc
      241 gggtacgtcg gcttcgacca gggcggactg ctgaccgagg ccgtgaccaa gcacccgcac
      301 agcgtgctgc tgctcgacga gatcgagaag gcgcacccgg acgtgttcaa cctgctgctg
      361 caggtcatgg atcacggcac gctgaccgac aacaacggcc gcaaggcgga tttccgcaac
      421 gtcatcctgg tcatgaccac caacgccggt gcagaccaga tcgagcgctc cggcatcggc
      481 ttcacgaccg aggatcactc gaccgacagc ctcgaagtca tcaagcgcat gttctcgccg
      541 gagttccgca accgcctcga tgcgatcatc cagttcaagg cgctggagag cgacacgatc
      601 ggccacgtcg tcgacaagtt catcatcgag ctcgaagcgc agctggttgc caagcacgtg
      661 acgctggaag tctccggcgc cgcccgcgcc tggctcgccg tgcgcggcta cgaccgcaag
      721 atgggcgccc ggccgatggc gcgcgtgatc caggagcaca tcaagaagcc gctggccgag
      781 gaactgctgt tcggacgcct cgcctcgggc gggcacgtgc tgatcgatgc cgacggtgac
      841 gggctggtga tcgatttcgc cgaggcgacg gcggaagtga gctgaccgga ccggcgctga
      901 cgccgcgtgc tgcaccggac cggcgggggc gaccctgccg gttcttgttt ttcagcggcc
      961 gcgataggtg atgcggcctt tcgacaggtc gtacggcgtc agttcgacgc ggaccttgtc
     1021 gccggtcagg atgcggatgt agtgcttgcg catgcggccg gaaatgtggg cggtgacgac
     1081 gtggccgttt tcgagctgta cgcggaacat cgtgttcgga agggtctcga tgaccgttcc
     1141 ttccatttcg atatggtctt ctttcgacat caggactctc tggcaggggg aaaagcggcg
     1201 gtgagggcga tcggtcggtg cggtcggtct ggtgcggcgc gccaaaatag ccaaacggcc
     1261 cttcagcatc aaggaccggc agcacgggac agggtgaatt cgtacgcgag ggtggacggt
     1321 cgcggccggg cggttcagcg gacaggcgtt tcgggcgggg agcgccagtc gccgttgatg
     1381 aagacctcgc agggccggaa gcgcgtcttg tagttcatct tgcggcagcc ggcaatccag
     1441 tatccgaggt agacgtaggc cagaccgcgg cggcgcgcct cctcgatctg ccagaggatc
     1501 gccgcggtgc cgggtgcccg cgcttcggcc gcgggatcgt aatacgtgta gaccgccgac
     1561 agtccgtccc cgagcacgtc gacgaccgcg accgccagca gccggccgtc ggcgtcgcgg
     1621 aattcggcga ggaaggtccg gctccagctg gaacggatga agccgaggta gctgtcgggg
     1681 gtggcatcgt ccatgccgcc gtccgggtgg cgcagcgcca ggtagcgggc gaacagctgg
     1741 aagtgctcct cgcgcagcac cggttcgacc aggctgacct gcaggtcgag gttgcgggcc
     1801 tgcgtgcgtc gctgcgagcg gttcggctgg aactgccgga ccgggatgcg cagcgactgg
     1861 caggcgtggc agccctggca gtgcggccgg tagacgtgct cgccgctgcg ccggaagccg
     1921 tgcagcacca gcgccgagta cagcgtgtcg cagacggcga gcgggtcggc gacgaggttg
     1981 cgcgccgtgt ggccggccag atagctgcac ggatgcgcat cggtcaggaa cagccgcagg
     2041 ctgccgagtt cggagcgcgg agcggccatc gtcagccggc tccgggcgct gcttcggccg
     2101 gcagcgtcca cggcgtggcc ggcagcaggc ggtggacgtg ctcgtgcagc agcttcagga
     2161 actcggcgcg tggcaggctc tgcgcgccga ggctcagcag atggccggtt tcgagctggc
     2221 agtcgatgaa gccgaagccg cgctgcacca gcagccggcc gagatgcacg agcgcgacct
     2281 tggacgcatc gttcatgcgg ctgaacatcg attcgccgaa gaacacgccg ccgagcgcga
     2341 cgccatacag cccgccgacc agacgctcgc cgtgccaggt ctcgatcgaa tgcgcgtagc
     2401 cgagcacgtg cagccggcag taggcctcgc gcatctcgcg ggtgatccag gattccgggg
     2461 cctccggtcg cggtgcggcg cattcggcga tcacgcgcgc gaaggcgtga tcgaccgtga
     2521 ccgtgtagcg accgctgcgc agcaccttgc gcaggctgtg cgagacccgg atgcggtcgg
     2581 gcaggaagat cgtgcgcggg tccggtgacc accacaggat cggctgtccc ttgccgtacc
     2641 acgggaagat gccctgccgg tagccgttca gcagccgggc cggcgccagg ctgccgccgg
     2701 cggcgagcag gccgttcggt tcgaccagcg ccgcctcggc gggcggaaac ggctggttcg
     2761 gattgcgggc gtcgagccag gcgatcatgc cgcggcctcg ccgctgcgat acggcagccg
     2821 tgcggtgagt tcgcgtgcgt actcgacgat ggccgggcgc tcgcgatgca ggaaggcgtc
     2881 gatcgcctga cggaagcccg gatgtgcgac ccagtgcgcc gagtgcgtca gcaccggttc
     2941 gaagccgcgc gccaccttgt gctcgccctg cgcgccgggt tcgaagcgtg tcagcccgtg
     3001 ctcgatgcag tgctcgatac cctggtagta acaggcttcg aagtgcagcg cgtcgaagga
     3061 ctcggcgcag ccccagtggc ggccatacag cgtgcgtgcg ccggcgtagc tgatcgcggc
     3121 ggcgacgatg tcatgcccgc gccgggcaag caccagcagc acgcgttcgc cgagcgtgcg
     3181 gccgatctcc aggaagaacg gcaggctcat cggggccgga cagccgcgct gctcgaaggt
     3241 gttgcggtag aagcggtgga agacccgcca ctgcgcctcg ctgacggcat tcccgggcag
     3301 gcgttcgagt tcgaggccgg cctcacgcac gcgccggcgt tcctggcgca ggttcttgcg
     3361 cttcttggcc gagaaggcgt cgaggaaatc gtcgaaatcg cggtagccgc gatcctgcca
     3421 gtggaactgg cagccgagcc ggtgcgtgag cccggccgcc gtcagggatt cgagttcacc
     3481 ggcctgcggg aacagcacgt gcagcgtcga gcagccgagt ccggcggcga gctcgcgcgc
     3541 cccttcggca agcagcgcca tgaccgcgcc ggcatcggca ccggcggcga ccagcagccg
     3601 ctggccggtc gccggcgtgt gcgggatcgc cgccaccagc ttcgggtaat agcgcaggcc
     3661 gttgcggtgg taggcatcgg cccagcccca gtcgaacacg aactcgcccc aggagtcggt
     3721 cttcaggaac agcggacacc ccccgagcag gcggccgtca tcgtcgcgca cgctcagcgg
     3781 tcgtggttgc cagtgcgtgc cggggccggt gacgccgtgg ttttcgagcg cgacgagaaa
     3841 cgcatgctga agaaaggggt cttcgtcgcg gaccagggtg ttccactcgg cagcgggcag
     3901 ctcgcggaag gattcgtggc agcgcaggtg catgctcttg gggggctcgt gtgaccggtg
     3961 ccgcttcgac gcgcggggcg cggcgaagtt tgacacccga ttccaagctt aggggcaagt
     4021 cgacggccgc gcacgtggtg cgcgctttgc cagcaccggg gcgggatggg acaatgcatc
     4081 ccctcatcac ccctccggct gctccgccgc cctgccgtcc gcttcgacgc gccttgcgcg
     4141 gcacggaccc atccccagat cgcttcacac aggtggcagc ttggctcagc aggcacgcag
     4201 aaaaaacgtg acgcccgcgg gcgtcaagat cgtccgtcac atcagccatg gcctgcgcga
     4261 gatcggcttc ctgctgctcg cgaccgtggc ggccttcctg ttcatcgcac tggcctcgta
     4321 cgtgccggcc gatccggcct ggacgcatac cggcaccagt gcaccgatga acctcggcgg
     4381 cgtggccggc gcgtggtttg ccgacatcgc gctgtacttc ttcggctacc ccgcctacct
     4441 gttcccgctc ggcctggtgt tcgcgggctg gcggctgttc aagcgcaacg gccttgccga
     4501 gatcgacgcc gagatcatcg tgttccgtac cgtcggcttt ctggtcacgg tcggcaccgg
     4561 ctgcggactg gccgccctgc acctgcagcc cggagccctg cgcggcagcg ccggcggcat
     4621 cgtcggcgat ctgtgcgggc acgggctgat caacgccttc ggacccggtg gcggcacgct
     4681 gttcctggcc gcgctgttcc tcgccggcgt gacgctggcc tcggggctgt cgtggctgtc
     4741 gctggtcgat ctgatcggca agtcgatctg ggatgtcgcg gcgctgttcg cccgggccgg
     4801 cagctgggtt gccgagcgca tcgctccgcg ccgggacgaa gccggcgacg atgagccact
     4861 gcagcagggc agggcgcccg agctgtcgct ggccggcggc cggcaggagc cggtgctggt
     4921 tgcagggctg ccgccgcagg cgccgcctgt gccggtgtcg aagccggtca cggaaccgga
     4981 aaaacctgcc accggcagcg acggcccggc cgagccggct accgccggca agcgcggccg
     5041 gatcgaaccg tccttcgatg cgacgacggc tccgtgggac ctcggggagg acgacgagcc
     5101 gaagccgccg ctgcc
//
LOCUS       DAOQJH010000085         5113 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_9556_length_5113_cov_5.728549, whole genome shotgun sequence.
ACCESSION   DAOQJH010000085 DAOQJH010000000
VERSION     DAOQJH010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 5113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5113
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9556_length_5113_cov_5.728549"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1043)
                     /locus_tag="PLQ18_03130"
     CDS             complement(<1..1043)
                     /locus_tag="PLQ18_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017454238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter ATP-binding protein"
                     /protein_id="HNE00674.1"
                     /translation="MSIRIHNVSKRFGPYTALDDISLDIPSGELVALLGPSGCGKTTL
                     LRIIAGMESPDAGQVWFDGTDTTHKHVRERRVGFVFQHYALFQHLSVFENVAFGLRVR
                     PRATRPAEAEIRAKVMELLQLVQLEGFAARYPAQLSGGQRQRVALARALAVEPQVLLL
                     DEPFGALDTQVRKELRRWLRRLHDELHVTSVFVTHDQEEALEVADRIVVMNRGHIEQI
                     GSPDAVYETPATPFVYQFLGNVNLFHSRVHDGIASIAGLRVPAPEHDDAEHAPALAYV
                     RPHDIDLSRTAGEAALAATVIEVRPLGPVVRLELKLDGDGEPLEVELTRERFQLEGYA
                     TGEPVWVRPRRMKV"
     gene            complement(1100..1981)
                     /gene="cysW"
                     /locus_tag="PLQ18_03135"
     CDS             complement(1100..1981)
                     /gene="cysW"
                     /locus_tag="PLQ18_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HNE00675.1"
                     /translation="MTPSASPVPVTIPAPRPARIEAATTEPRLVRWLLCALAFGFLTL
                     FLFVPLLAVFVEALGKGFALYWKALSDAEALAALRLSLLATVIAVPLNLVFGVAAAWA
                     IARFEFRGKAALVTLIDLPFAVSPVIAGLIYVLVFGAHGWFGPWLAGHDLKVIFAVPG
                     IVLATVFVTFPFVARELIPLMQALGSEQEEAAIVLGASGWQTFRRVTLPCIRWGLLYG
                     TVLSTARALGEFGAVSVVSGHIRGLTTTLPLHVEILYNEYNFTAAFACASVLTLSALL
                     MLAAKSAIEWRTRRHGE"
     gene            complement(2020..2883)
                     /gene="cysT"
                     /locus_tag="PLQ18_03140"
     CDS             complement(2020..2883)
                     /gene="cysT"
                     /locus_tag="PLQ18_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012353040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HNE00676.1"
                     /translation="MAALSLLSPTTARTPRVLPGFGLALGITLSYLALIVLIPLSATF
                     LKAAGLSWDGFVNAVTAPRVLASYRLTFGAALIAALINAVFGLIVAWVLVRYRFAGRR
                     FVDALVDLPFALPTAVAGIALTALYSTNGWIGQWLAGFGIKVAFTPLGVIVALTFIGL
                     PFVVRTVQPVLEEFDRGVEEAAAVLGASRWQTFTRVILPALLPALVTGFTMAFARAVG
                     EYGSVIFIAGNIPLVSEITPLIIITKLEQYDYAGATAVACVMLVISFVLLLAINGLQA
                     WNARRRSAAAA"
     gene            complement(2885..3907)
                     /locus_tag="PLQ18_03145"
     CDS             complement(2885..3907)
                     /locus_tag="PLQ18_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HNE00677.1"
                     /translation="MTLLPLRAALFGWLSACCLAGSGNVNAADLELLNVSYDPTRELY
                     QAYNAAFVREWQAGHPGDTLTIRQSHGGSGKQARSVIDGLAADVVTLALAGDIDAVAR
                     SGLIAGDWQKRLPHASTPYYSTIVFLVRKGNPKGLKDWGDLARPGVAVITPNPKTSGG
                     ARWNYLALWGWALRQPGGSEDSARALLEKVFRNVPVLDTGARGSTTTFVQRGIGDVLL
                     AWENEALLALKEYGSDAFEIVVPKTSIRAEPPVAVVDKVVERRGTQTLADAYLRGLYE
                     PAAQRIAAEHGYRPTDAAVAAEFRTRFLQIELFGIDDFGGWSAVQSKHFADGGEFDRL
                     YQPGSR"
     gene            3946..>5113
                     /locus_tag="PLQ18_03150"
     CDS             3946..>5113
                     /locus_tag="PLQ18_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 factor interaction domain-containing
                     protein"
                     /protein_id="HNE00678.1"
                     /translation="MSKRRADFAIFNNSMMGFPKQNIPYEYSCCFIRHIFWYLSYLLI
                     YPSNMLEHHSSDEPTQALLLALLDLWQFVARPAAAGRLDAVLPDITRILGRALPVTAL
                     ELLIPDAGNQQLRGWRCEAADETRSSRCPLPAAELSALTSWCAASTIETTNGRSRPRW
                     LKALPTLSASRADLVSGAGPMAALGLAGSAGEHSALALLHLRAGAGIGPGLRGCLARI
                     AAGLRTLLDWRDVRSELSGLHELVSQLRDREPVRSTETIVGADGGLRPVLERVRLVAA
                     TDVPVLILGETGSGKEVIARAIHQGSPRTGQAFVRVNCGALPPDLIDTELFGHERGAF
                     TGAAAARRGWFEQADRGTLFLDEIGELPLAAQVRLLRVLQDGTLNRIGSETPLNV"
BASE COUNT          845 a         1708 c         1818 g          742 t
ORIGIN      
        1 accttcatgc gccgcggccg cacccagacc ggttcgccgg tggcgtagcc ttcgagctgg
       61 aagcgctcgc gggtcagttc gacttcgagc ggctcgccat cgccgtcaag cttcagctcc
      121 aggcgcacga ccggcccgag cgggcgcacc tcgatgaccg tcgccgccag cgcggcctca
      181 ccggcagtgc ggctcaggtc gatgtcgtgc gggcgcacgt aggcgagtgc cggcgcgtgc
      241 tcggcgtcat cgtgctccgg cgccggcacg cgcaggccgg cgatgctggc gatgccgtcg
      301 tgcacgcgcg aatggaacag gttgacgttg ccgaggaact ggtacacgaa cggcgtggcc
      361 ggggtctcgt agacggcatc gggcgagccg atctgctcga tgtggccgcg gttcatgacc
      421 acgatgcgat cggcgacctc cagggcttcc tcctgatcgt gggtcacgaa cacgctggtc
      481 acgtgcagct cgtcgtgcag ccggcgcagc cagcggcgca gctccttgcg cacctgggtg
      541 tcgagggcgc cgaagggctc atcgagcagc agcacctgcg gctcgaccgc gagcgcccgc
      601 gccagtgcca cgcgctggcg ctggccgccg gagagctgcg cggggtagcg cgcggcgaag
      661 ccctcgagct gcacgagctg cagcagttcc atgaccttgg cgcggatctc ggcttcggcc
      721 gggcgcgtcg cccgcgggcg cacgcgcagg ccgaaggcga cgttctcgaa cacgctcagg
      781 tgctggaaca gcgcgtagtg ctggaacacg aagccgacgc ggcgctcgcg cacgtgcttg
      841 tgcgtggtgt cggtgccgtc gaaccagacc tggccggcgt ccggactttc catgccggcg
      901 atgatgcgca gcagcgtcgt cttgccgcag cccgaaggtc cgagcagcgc gaccagctcg
      961 ccggaaggga tgtcgaggct gatgtcatcg agcgcggtgt acgggccgaa gcgcttggag
     1021 acgttgtgga tgcggatgct catggcagcg gaatctccgg cgtctggcga aagaggcgag
     1081 ggcagcgggc ggtgcggctt cattcgccgt ggcggcgcgt gcgccattcg atggcgctct
     1141 tggctgcgag catgagcagc gccgacagcg tcagcaccga ggcgcaggcg aaggcggccg
     1201 tgaagttgta ttcgttgtag agaatctcga cgtgcagcgg cagcgtcgtg gtcaggccgc
     1261 ggatgtggcc ggagacgacg ctgacggcgc cgaactcgcc gagcgcgcgt gcggtgctga
     1321 gcacggtgcc gtagagcagg ccccagcgga tgcacggcag cgtcacgcgc cggaaggtct
     1381 gccagccgct cgccccgagc acgatcgccg cctcttcctg ctcgctgccg agcgcctgca
     1441 tcagcgggat cagctcgcgg gcgacgaacg ggaaggtcac gaacaccgtt gccagcacga
     1501 tgccgggcac ggcgaagatc accttcaggt cgtgtccggc cagccacggc ccgaaccagc
     1561 cgtgcgcacc gaacaccagc acgtagatca ggccggcgat caccgggctg accgcaaacg
     1621 gcagatcgat cagcgtcacc agcgccgcct tgccgcggaa ctcgaagcgc gcgatcgccc
     1681 aggcggcggc gaccccgaac accaggttca gcggcacggc gatcacggtc gccagcagcg
     1741 acaggcgcaa tgccgccagc gcctcggcat cgctcagcgc cttccagtac agcgcgaagc
     1801 ccttgccgag cgcctcgacg aacacggcga gcagcggcac gaacaggaac agcgtcagga
     1861 agccgaaggc cagcgcacaa agcagccagc gcaccaggcg cggttcggtg gtggccgcct
     1921 cgatgcgggc ggggcggggc gccgggatcg tgaccggcac gggcgaagcg gaaggggtca
     1981 tgacggcact catggcggtc gtcgctccgg agtcggggat caggcggccg cagccgagcg
     2041 gcggcgggcg ttccaggcct gcaggccgtt gatcgcgagc agcagcacga aggagatcac
     2101 cagcatcacg caggcaacgg cggtggcacc ggcgtagtcg tactgttcga gcttggtgat
     2161 gatgatcagc ggcgtgatct ccgagaccag cggaatgttg ccggcgatga agatcaccga
     2221 gccgtactcg ccgacggcgc gcgcgaaggc catcgtgaag ccggtgacca gcgccggcag
     2281 cagcgccggc aggatcacgc gcgtgaaggt ctgccagcgg ctggccccga gcacggcggc
     2341 cgcctcctcg acgccgcggt cgaactcctc cagcaccggc tgcaccgtgc gcacgacgaa
     2401 gggcaggccg atgaaggtca gcgccacgat cacgccaagc ggcgtgaacg ccaccttgat
     2461 gccgaagccc gcgagccact ggccgatcca gccgttggtc gaatacagcg ccgtcagcgc
     2521 gatgccggcg accgccgtcg gcagcgcgaa cggcagatcg accagcgcat cgacgaaccg
     2581 ccggcccgca aagcggtagc gcaccagcac ccaggcgacg atcagcccga acacggcatt
     2641 gatcagggcg gcgatcagcg ccgcaccgaa ggtcagccgg tacgaggcca ggacacgcgg
     2701 cgccgtgacc gcgttgacga agccgtccca gctcaggccg gcagccttca ggaaggtcgc
     2761 cgacagcggg atcagcacga tcagcgcgag gtagctcagc gtgatgccga gcgccagccc
     2821 gaagccgggc agcacgcgcg gcgtgcgcgc ggtcgtcggt gacagcagtg acagcgccgc
     2881 catgtcagcg gctgccgggc tggtagagac ggtcgaactc gccgccatcg gcgaagtgct
     2941 tgctctgcac ggcgctccag ccgccgaagt cgtcgatgcc gaacagctcg atctgcagga
     3001 agcgggtgcg gaattcggcg gcgaccgccg catcggtggg ccggtagcca tgctcggccg
     3061 cgatgcgctg ggcggccggc tcgtagagcc cgcgcaggta ggcgtcggcg agcgtctgcg
     3121 tgccgcgtcg ttcgacgacc ttgtcgacga ccgccaccgg cggctcggcc cggatgctgg
     3181 tcttcggcac aacgatctcg aaggcgtcgc tgccgtattc cttcagcgcc agcagggctt
     3241 cgttctccca ggcgagcagc acgtcgccga tgccacgctg cacgaaggtc gtcgtcgagc
     3301 cgcgggcgcc ggtgtcgagc acgggcacgt tgcggaagac cttttcgagc agggcccggg
     3361 ccgagtcctc gctgccgccg ggctgccgga gtgcccagcc ccacaatgcc aggtagttcc
     3421 agcgggcacc gccggaggtc ttcggattcg gcgtgatcac cgccaccccg ggccgtgcca
     3481 gatcgcccca gtccttcagg cccttcgggt tgcccttgcg caccaggaac acgatcgtcg
     3541 agtagtacgg cgtgctggcg tgcggcaggc gcttctgcca gtcgccggcg atcaggcccg
     3601 atctggccac ggcgtcgatg tcaccggcca gggccagcgt cacgacatcg gcggcgagcc
     3661 cgtcgatgac cgagcgcgcc tgcttgccgg aaccgccatg ggactggcgg atcgtcagcg
     3721 tgtcgcccgg atgcccggcc tgccactcgc gcacgaaggc ggcgttatag gcctgataca
     3781 gctcgcgcgt cggatcgtag gaaacattca gcagttcaag atcggcggca ttcacgttgc
     3841 cgctgccggc gaggcagcag gccgacagcc agccgaacag ggcggcgcgc aagggcagga
     3901 gagtcatgga tcaccgtacg aagcggaaag agcgggtacg gtgggatgag caaacggcgt
     3961 gccgacttcg cgatttttaa taactcgatg atgggttttc caaaacaaaa tattccatat
     4021 gaatattcat gctgttttat ccgtcatata ttctggtatc tatcttattt actgatatat
     4081 ccgtcaaata tgctcgaaca tcattcttcc gatgaaccga cgcaagccct cttgctggcc
     4141 ttgctcgacc tctggcagtt cgtcgcccgc ccggcggcgg cgggacggct ggatgccgtg
     4201 ctgccggaca tcacgcgcat cctgggccgg gccctgccgg tcaccgcgct ggagctgctg
     4261 attcccgatg ccggcaatca gcagctgcgt ggctggcgct gtgaggctgc ggacgaaacc
     4321 cgttcctcgc gctgcccgct gccggctgcc gagctcagtg cactgacgag ctggtgtgcc
     4381 gcctcgacga tcgaaacgac taacggccgc agccggccgc gctggctcaa ggccctgccg
     4441 acgctgtcgg catcgcgcgc cgatctcgtg tccggcgcgg ggccgatggc tgcactggga
     4501 ctggccggct ctgccggaga acacagtgcg ctggcgctgc tgcatctgcg ggcgggagcc
     4561 gggatcggac ccggcctgcg gggctgtctg gcgcgcatcg cggcgggttt gcgcaccttg
     4621 ctcgactggc gggacgtgcg cagcgaactg agcgggctgc acgaactcgt cagccagctg
     4681 cgcgaccgcg agccggtgcg cagcacggaa accatcgtcg gcgctgatgg cgggctgcgg
     4741 ccggtgctgg agcgcgtgcg cctggtggcg gcgaccgatg tgccggtgct gatcctcggc
     4801 gagaccggct cgggcaagga ggtcatcgcc cgtgcgatcc accagggctc gccgcgcacc
     4861 ggccaggcct tcgtgcgcgt caactgcggg gcactgccgc cggatctgat cgataccgag
     4921 ctgttcggac acgaacgtgg cgccttcacc ggtgctgctg cagcccggcg tggctggttc
     4981 gagcaggcgg atcgcggcac gctgttcctg gacgagatcg gcgaactgcc gctcgccgcg
     5041 caggtgcgcc tgctgcgcgt gctgcaggac ggcacgctga accgcatcgg tagtgaaacc
     5101 ccgctgaacg tca
//
LOCUS       DAOQJH010000086         5107 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_9573_length_5107_cov_4.763064, whole genome shotgun sequence.
ACCESSION   DAOQJH010000086 DAOQJH010000000
VERSION     DAOQJH010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 5107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5107
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9573_length_5107_cov_4.763064"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="PLQ18_03155"
     CDS             <1..297
                     /locus_tag="PLQ18_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020484792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KOW motif-containing protein"
                     /protein_id="HNE00679.1"
                     /translation="VPKVLGFIGGTSDKPAPISEKEAQAILQRVQEGADKPKPKVLYE
                     PGEMVRVTDGPFNDFEGVVEEVNYEKSRLRVGVMIFGRTTPVELEFGQVEKVSR"
     gene            391..822
                     /gene="rplK"
                     /locus_tag="PLQ18_03160"
     CDS             391..822
                     /gene="rplK"
                     /locus_tag="PLQ18_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015819278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HNE00680.1"
                     /translation="MAKKVTAYVKLQVAAAKANPSPPVGPALGQHGVNIMEFCKTFNA
                     QTQGMEVGMPIPVVITVYSDRSFTFIMKTPPATYLIKKAAGITSGSSNPNTKKVGTIS
                     RAQLEEIARVKQPDLTAASIDAAVRTLAGSARSMGLNVEGV"
     gene            826..1527
                     /gene="rplA"
                     /locus_tag="PLQ18_03165"
     CDS             826..1527
                     /gene="rplA"
                     /locus_tag="PLQ18_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HNE00681.1"
                     /translation="MAKLSKRQQAIRAKLVPGKVYAAEEAFSFLKELASAKFRESIDV
                     SVNLGVDPRKSDQVVRGSTVLPNGTGKTVRVAVFTQGANAEAARAAGADIVGFEDLAE
                     SVKGGNLDFDVVIASPDAMRIVGQLGQILGPRGLMPNPKVGTVTPDVAGAVRNAKAGQ
                     VRYRTDKAGIIHCTLGKVDFEPAKLRENLAALLADLNKMKPSAAKGVYMKKVTVSTTM
                     GPGLAVDVASVTAAA"
     gene            1849..2391
                     /gene="rplJ"
                     /locus_tag="PLQ18_03170"
     CDS             1849..2391
                     /gene="rplJ"
                     /locus_tag="PLQ18_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012983541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HNE00682.1"
                     /translation="MSLNLAEKQAVVAEVAEVAARSHSLIAAEYRGITVARLTNLRVK
                     ARENGVYLRVVKNTLARRAVQGTDFECISPSLVGPLVLAFSEEDPGAAARVFKEFLKE
                     KDNDKLIVKALAFGGQLLGPQDLDALASMPTRDQAISLLMAVMKAPLDKFARTLNEIP
                     GKLVRTVEAIRVQKAESEAA"
     gene            2452..2829
                     /gene="rplL"
                     /locus_tag="PLQ18_03175"
     CDS             2452..2829
                     /gene="rplL"
                     /locus_tag="PLQ18_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010960365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HNE00683.1"
                     /translation="MAVSKEEMLDTIANMTVIEIVDLIKAMEEKFGVSAAAAVAAAPA
                     AAAAAAAPVEEQTEFNVVMTSFGENKVNVIKVIRTITGLGLKEAKDLVEGVPSTVKEA
                     IAKADAEKIKKDLEEAGAKVEIK"
     gene            3053..>5107
                     /gene="rpoB"
                     /locus_tag="PLQ18_03180"
     CDS             3053..>5107
                     /gene="rpoB"
                     /locus_tag="PLQ18_03180"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005224648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HNE00684.1"
                     /translation="MAYTFTEKKRIRKDFGKRPSILEVPYLLEIQIDSYREFLQADLP
                     PEARREVGLHAAFRSVFPITSYSGNAQLEYVSYELGEPVFDVRECQLRGLTYAQPLRV
                     KLRLVIFDKESDNKAIKKVIEPESVYMGEVPIMTENGTFVINGTERVIVSQLHRSPGV
                     FFDHDRGKTHSSGKLLFSARVIPYRGSWLDFEFDAKDIVYARIDRRRKLPVTILLRAL
                     GYDNQQILDLFFETNTFRLQDGRVLLDLVPERLKGETASFDIRVGDQVIVEAGRRITV
                     RHIRELTKAGVTTLEVPADYLLGRILAHDVIDTRTGELVLLANEELSVDHLEKLGAAG
                     ISEFRTLFVNDLDRGAFISQTLRVDPTRSELEAQVEIYRMMRPGEPPTREAAQNLFHN
                     LFFSEDRYDLSAVGRMKFNRRVGREAVTGPGVLYDHQYCAKRSEELLKKLVAEYGVTS
                     DIIDVLRVLIGIRNGSGQVDDIDHLGNRRIRCVGEMAENVFRVGLVRVERAVKERLSV
                     AESENLGPRDLINAKPVAASIKEFFGSSQLSQFMDQNNPLSEVTHKRRVSALGPGGLT
                     RERAGFEVRDVHATHYGRVCPIETPEGPNIGLINSLAVYARTNEYDFLETPYRKVDNG
                     CVTDRIDYLSAIEEGKYVIGQANAALDPDGRLIDSLVSCRVQGEFTLRSPDQVEYMDV
                     SPK"
BASE COUNT          929 a         1689 c         1678 g          811 t
ORIGIN      
        1 gtgcccaagg tgctcggttt catcggtggg accagtgaca agccggcgcc gatttccgag
       61 aaggaagccc aagccatcct gcagcgcgtc caggaaggtg ccgacaagcc caagccgaag
      121 gtcctttacg agcccggcga gatggtgcgg gtgacggacg gtcccttcaa tgattttgaa
      181 ggcgtggtcg aggaagtcaa ttacgagaag agccggctgc gcgtaggtgt gatgattttc
      241 ggtcgcacta ctccggtcga actcgaattc ggccaggtcg aaaaagtatc gcgttgaccg
      301 gcatccgccc ggtcacgccc ttgaccgcac gcgcaaccgc gcggcgtatt ccggggagcc
      361 gcaaggcgtt cgcacccgac aggagcactc atggcaaaga aagttaccgc atacgtcaag
      421 ctacaggtag cggccgccaa ggccaacccc agcccgccgg tcggtccggc gctcggccag
      481 cacggcgtca atatcatgga attctgcaag accttcaatg cccagaccca gggcatggaa
      541 gtcggcatgc cgattccggt cgtgatcacg gtctacagcg accgcagctt cacgttcatc
      601 atgaagacgc cgccggcgac ctatctgatc aagaaggccg cgggcatcac ctcgggcagt
      661 tcgaatccga acaccaagaa ggtcggcacg atcagccggg cgcagctcga agagatcgcc
      721 agggtcaagc agcccgacct gaccgccgcc agcatcgacg ccgcggtgcg cacgctcgcc
      781 ggcagcgccc ggtcgatggg cctcaacgtg gagggggtct gagtcatggc caagctcagc
      841 aagcgtcagc aggcgatccg cgccaagctc gttccgggca aggtctatgc ggccgaggaa
      901 gccttctcgt tcctgaagga actcgcgagc gccaagttcc gtgagtccat cgacgtctcg
      961 gtgaacctcg gcgtcgatcc gcgcaagtcc gaccaggtgg tgcgcggctc gaccgtgctg
     1021 ccgaacggca ccggcaagac cgtgcgcgtc gcggtgttca cgcagggtgc gaacgccgag
     1081 gcggcgcgtg cggccggcgc tgacatcgtc ggcttcgagg accttgccga gagcgtcaag
     1141 ggcggcaacc tcgacttcga cgtcgtcatt gcctcgccgg atgcgatgcg catcgtcggc
     1201 cagctcggcc agatcctcgg cccgcgcggc ctgatgccga acccgaaggt cggcaccgtg
     1261 accccggacg tcgccggcgc cgtgcgcaac gccaaggccg gtcaggtgcg ctaccgcacc
     1321 gacaaggccg gcatcatcca ctgcacgctc ggcaaggtcg acttcgagcc ggcgaagctg
     1381 cgcgagaacc tcgccgccct gctcgccgac ctcaacaaga tgaagccgtc cgccgccaag
     1441 ggcgtgtaca tgaagaaggt cacggtctcg accacgatgg gccccggcct ggccgtggac
     1501 gtggcttcgg tgactgccgc ggcctgatcg ggccgcctgc gtgccggtgc gccagtcgcg
     1561 cgccggcgcg cccgaacttt gggcggccgg cagcttgctg ccggccggcg tcaaagaccg
     1621 ctggtgccgc aaggcttaat gaccgtcgga tgacggcgcc cgcgcagacg gtctcgccca
     1681 tccggccatg cgccggagca ggcttgccgt tgggagggcc ggcatcgcac cccgtgcggg
     1741 gtgaggccgg ttttgggttc taccgttgcc gtgaacggcg cggaggctgt cgcagggcag
     1801 ccctgcagtc agtcgctgca gacgtgccgt tccagagggt agttgttcat gagcttgaat
     1861 ctggcagaga agcaggccgt cgtcgccgaa gtcgccgagg ttgccgccag gtcgcactcg
     1921 ctgatcgctg cggaataccg cggcatcacc gtggcgcgcc tgaccaacct gcgcgtcaag
     1981 gcgcgtgaga acggcgtcta tctgcgggtc gtcaagaaca cgctggcgcg ccgcgccgtg
     2041 cagggcaccg atttcgagtg catctcgccg tcgctggtcg ggccgctggt gctcgcgttc
     2101 tcggaagagg acccgggtgc cgccgcgcgc gtcttcaagg agttcctgaa ggagaaggac
     2161 aacgacaagc tgatcgtcaa ggcactcgcc ttcggcggcc agctgctcgg tccgcaggat
     2221 ctcgacgcgc tcgccagcat gccgacccgt gaccaggcca tcagcctgct gatggcggtc
     2281 atgaaggcgc cgctcgacaa gtttgcgcgc accctcaacg agattccggg caagctggtc
     2341 cggaccgtcg aggcgatccg cgtgcagaag gccgagtccg aggccgcctg aggcggtttc
     2401 gtacccgcac cccgtatacc gaacacgaat tcctgatctg gagtattcga aatggccgtt
     2461 tcgaaggaag aaatgctgga taccatcgcc aacatgaccg tgatcgagat cgtcgatctg
     2521 atcaaggcga tggaagagaa gttcggcgtc tccgccgctg ccgccgttgc cgccgccccg
     2581 gccgctgccg ctgccgctgc cgcaccggtc gaggagcaga ccgagttcaa cgtcgtgatg
     2641 acctcgttcg gcgagaacaa ggtcaacgtc atcaaggtga tccgcacgat caccggtctg
     2701 ggcctgaagg aagcgaagga cctggtcgaa ggcgtgccct cgaccgtcaa ggaagccatc
     2761 gccaaggccg atgccgagaa gatcaagaag gatctggaag aggccggtgc caaggtcgag
     2821 atcaagtaag tactcgaccg agcccgcgca ggccgatggc ggcttgcgcg gggctgaccg
     2881 ggacgggctg gtggcgccac tgcgccgccg gcctttccct gtttaggcgc cgctgcaacg
     2941 gtgcctgcgc ggatactccg acaccggaca agtccggctc cgcgataggt cggaaaggtg
     3001 catgggcccg cgcgtcaggc cgtgcccgaa cccgttttga cgaggaaccc cgatggccta
     3061 caccttcacc gaaaagaagc gcattcgtaa ggacttcggc aagcgcccga gcatccttga
     3121 ggtgccgtac ctgctcgaga tccagatcga ctcgtatcgc gagttccttc aggccgatct
     3181 cccgccggaa gcgcgtaggg aggtggggct gcatgcggcg ttccggtcgg tgttcccgat
     3241 caccagctat tccggcaacg cgcagctcga atacgtcagt tacgagctcg gcgagccggt
     3301 gttcgacgtg cgcgaatgcc agctgcgcgg cctgacctat gcgcagccgc tgcgcgtgaa
     3361 gctgcgcctg gtgatcttcg acaaggagtc cgataacaag gccatcaaga aggtcatcga
     3421 gcccgaaagc gtgtacatgg gcgaagtgcc gatcatgacc gagaacggca ccttcgtgat
     3481 caacggcacc gagcgcgtca tcgtctcgca gctgcaccgt tcgcccggcg tgttcttcga
     3541 tcacgaccgc ggcaagacgc actcctcggg caagctgctg ttctcggcgc gggtgattcc
     3601 gtaccgcggc tcctggctcg acttcgaatt cgacgcgaag gacatcgtct acgcgcgcat
     3661 cgaccgccgg cgcaagctgc cggtgacgat cctgctgcgc gcgctcggtt acgacaacca
     3721 gcagatcctc gacctgttct tcgagaccaa caccttccgg ctgcaggacg gccgcgtgct
     3781 gctcgatctc gtgcccgagc gcctgaaggg cgagacggcg agcttcgaca tccgggtcgg
     3841 tgaccaggtc atcgtcgagg ccggccggcg catcaccgtg cgccacatcc gcgagctgac
     3901 caaggccggc gtgacgacgc tggaagtccc ggccgactac ctgctcgggc gcatcctcgc
     3961 gcacgacgtg atcgacacgc gcaccggcga actcgtgctg ctcgccaacg aggagctgag
     4021 cgtcgatcac ctcgaaaagc tcggcgccgc cggcatcagc gagttccgca cgctgttcgt
     4081 caacgacctc gaccgcggtg cgttcatctc gcagacgctg cgcgtcgatc cgacccgctc
     4141 cgagctggag gcgcaggtcg agatctaccg catgatgcgt ccgggcgaac cgccgacccg
     4201 cgaggcggcg cagaacctgt tccacaacct gttcttctcg gaagaccgct acgacctgtc
     4261 ggcggtcggg cgcatgaagt tcaaccgccg cgtcgggcgc gaggcggtga ccggtccggg
     4321 cgtgctctat gaccaccagt actgcgcgaa gcgctccgag gagctgctga agaagctggt
     4381 cgccgaatac ggcgtgacct ccgacatcat cgacgtgctg cgcgtgctga tcggcatccg
     4441 caacggcagc ggccaggtcg acgacatcga ccatctcggc aaccgccgca tccgctgcgt
     4501 cggcgagatg gccgagaacg tgttccgcgt cggtctggtg cgcgtcgagc gcgcggtcaa
     4561 ggagcgcctg tcggtggccg agagcgagaa cctcggcccg cgcgatctca tcaacgccaa
     4621 gccggtggcc gcgtcgatca aggagttctt cggctcctcg cagctcagcc agttcatgga
     4681 ccagaacaac ccgctgtccg aggtcacgca caagcgccgc gtctcggcgc tcggccccgg
     4741 cggcctgacc cgcgagcgcg ccggcttcga ggtgcgcgac gtgcatgcga cgcactacgg
     4801 ccgcgtctgc ccgatcgaga ccccggaagg tccgaacatc ggcctgatca actcgctggc
     4861 cgtgtacgcg cgcaccaatg agtacgactt cctggagacc ccgtaccgca aggtcgacaa
     4921 cggctgcgtc accgaccgca tcgactacct gtcggcgatc gaggagggca agtacgtgat
     4981 cgggcaggcc aacgccgcgc tcgatccgga cggccgcctg atcgattcgc tggtgtcgtg
     5041 ccgcgtgcag ggtgagttca cgctgcgctc gccggatcag gtcgagtaca tggacgtgtc
     5101 gccgaag
//
LOCUS       DAOQJH010000087         5076 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_9653_length_5076_cov_3.990440, whole genome shotgun sequence.
ACCESSION   DAOQJH010000087 DAOQJH010000000
VERSION     DAOQJH010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 5076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5076
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9653_length_5076_cov_3.990440"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..581)
                     /locus_tag="PLQ18_03185"
     CDS             complement(<1..581)
                     /locus_tag="PLQ18_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE00685.1"
                     /translation="MDAVELPAADESPVRADVAPAAAHAVADPAAGPDTGAEAPDRSA
                     LVSRLRQRRERLVHVPAPVVEGRLTRLVGLTLEAVGCVAPIGAHCRVVSPGHGEPVEA
                     EVVGFAEDRVFLMPAGDIHGLAPYARVVPSGGGSRVPLGDGLLGRVLDGNGRMLDGWP
                     APLDVRPQPLAGRRLHPLERRPIREPLDVGVRA"
     gene            complement(581..1462)
                     /locus_tag="PLQ18_03190"
     CDS             complement(581..1462)
                     /locus_tag="PLQ18_03190"
                     /inference="COORDINATES: protein motif:HMM:NF014196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HNE00686.1"
                     /translation="MSDRIIPRQAGRDLPRWQFPGFDKAQPEAAPEPVAAPPGKSAGA
                     PLPEPEPSIPMPRLPTLEEIEAIERAAREEGYAAGRAEGRAKGYAEGHAEGRDAGFVE
                     GRAAGYADGSVAGRETGYAGGYESGHREGLAAGREEGREHGHREGLEAGAADIAARRA
                     LLETAMNELAAPLAALDAALEQELLALVVALARQLIRRELKTAPGEIVATIRETIPLL
                     PVTERQVLLHLHPEDAALIRELGTVAGGWQIVEDGTLSRGGCRLESGHSRIDATVEAR
                     LNTAIVALLGGEREEDH"
     gene            complement(1455..2444)
                     /gene="fliG"
                     /locus_tag="PLQ18_03195"
     CDS             complement(1455..2444)
                     /gene="fliG"
                     /locus_tag="PLQ18_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002808955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HNE00687.1"
                     /translation="MSGPDRVAVLMMALGEEYAAELLKQFDPRELHRIGASMASLSSV
                     GRDAVSTVVAEFNNELVNQNALVIDSRDFMKNVLTRALGRDKAKNVMDRILNEAGATG
                     VEALKWMEAQAVAAGLRREHPQIIALVLSYLESAQAAEVLALLPETLRHDVLIRVATL
                     DSVPTAAIGELNEVIEKQVMRNINAASSFKVGGPKRAAEILNAIDTGISTPLMDKIKG
                     MDESLALRIEDLMVVFEHLLNADDRGIQNLLREVSSETLIVAMRGADETIREKILRNM
                     SKRAAEMMRDDLESMGPVRLADVEAAQKDIMTIAKRMAEAGDLNLGSGGGGYV"
     gene            complement(2472..4502)
                     /gene="fliF"
                     /locus_tag="PLQ18_03200"
     CDS             complement(2472..4502)
                     /gene="fliF"
                     /locus_tag="PLQ18_03200"
                     /inference="COORDINATES: protein motif:HMM:TIGR00206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HNE00688.1"
                     /translation="MTEDTSLATATDTADAAPARAGWRDWLVRVRTPGPWMYGAIALG
                     VLLLATIVAVALSAGRPDFQPLYHGLAEADAGQIMEALNKMQVPVRVDRQTGTLNVPA
                     DRVAELRLKLAAQGLPRGTTGGFDTFEQQSGFGTSQFMENARYQHALEGELARSIMSI
                     GAVANARVHLALPKDTVFVRNKQQPTASVLVQLQPGRAIDAGQVAAIVHLVSSSVPRL
                     TPSQVSVVDQAGNLLTRPAGGTPGLNLDQMEYQQRLESNYTRRIEDLLTPILGANKMR
                     AQVALDLDFSQLEETSESFDPKRERGMVRSEELIEENNNRPQPMGIPGALSNQPPGVA
                     TAPEQAAGAPAAQADPNNPNAPQPGGGQTGNGQIGPQGQAAAAAGAQQSDAQRAADGS
                     RPMRTRSQSTRNYELDKRVSHITAAPGRVRRVSVAVVLDDRITVRPEGSVERVSWPQE
                     DIDRITALVREAVGFDAQRGDTVNVMNATFATTPFDPNLLPFWRQDWFIELVKWSLVA
                     IVAIVFLLVVVRPVVRRLLPPPPALPAPEPAAAAALAAPAATETGPGTALATTDAGGK
                     ALVPAEGGEASGDEAAAGGDEDADAVQLSAAGQALAQADGHHGNGNGKAPVPLPQPDD
                     AEAIERLEERLDIARRLVAEDPRRAVSVLRTWMNRQPGEGMALLASQEEEEA"
     gene            complement(4555..4872)
                     /gene="fliE"
                     /locus_tag="PLQ18_03205"
     CDS             complement(4555..4872)
                     /gene="fliE"
                     /locus_tag="PLQ18_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HNE00689.1"
                     /translation="MTLSPLNASIAALRAAATDTGAAAGPEAGAGDADFASLLKGALD
                     RVDARQQQASRMARAFELGEEGADLPDVMIAMQQSRLSFQSALQVRNKLVAAYQDILN
                     MPL"
BASE COUNT          640 a         1823 c         1842 g          771 t
ORIGIN      
        1 atcgcgcgca cgccgacatc gagcggctcg cggatcggcc ggcgttcgag cggatgcagg
       61 cgacggccgg cgagcggctg cggacgcacg tcgagcggcg ccggccagcc gtcgagcatg
      121 cggccgttgc catcgagcac gcgcccgagc agcccgtcgc cgagcggcac gcggctgccg
      181 ccgccgctcg gcacgacgcg cgcatacggc gccagaccgt ggatgtcgcc ggccggcatc
      241 aggaacacgc gatcctcggc gaagccgacc acctcggcct cgaccggctc gccgtggccg
      301 gggctgacga cgcggcagtg cgcaccgatc ggcgcgacgc agccgacggc ttcgagcgtg
      361 agccccacca gccgcgtcag ccttccttcg accaccggtg ccggcacgtg cacgagccgc
      421 tcgcgacgct ggcgcaggcg cgagaccagc gccgaacgat ccggcgcctc ggccccggtg
      481 tcggggccgg ctgcgggatc ggccacggcg tgcgcggcgg ctggcgccac gtccgccctg
      541 accggggatt cgtccgctgc cggcagctcg acggcgtcca tcagtgatcc tcctcgcgct
      601 cgccgccgag cagcgcgacg atcgcggtgt tcagccgggc ctcgacggtc gcatcgatgc
      661 gcgaatgccc ggattcgagc cggcagccgc cgcggctcag cgtgccgtcc tcgacgatct
      721 gccagccgcc ggcgacggtg ccgagctcgc ggatcagcgc cgcgtcttcc ggatgcaggt
      781 gcagcagcac ctggcgttcg gtgaccggca gcagcggaat ggtttcgcgg atggtcgcga
      841 cgatctcgcc gggcgcggtc ttcagttcgc ggcggatcag ctggcgcgcc agcgccacga
      901 ccagcgccag cagctcctgt tcgagcgcgg catcgagcgc ggcgagcggc gcggcgagtt
      961 cgttcatcgc ggtttcgagc agcgcgcggc gcgcggcgat gtcggccgcc ccggcttcca
     1021 gcccctcgcg atgcccgtgc tcgcggcctt cctcgcgccc ggcggcgagc ccctcgcggt
     1081 gaccggactc gtagccgccg gcgtagccgg tctcgcgtcc ggcgaccgag ccgtcggcgt
     1141 acccggcggc gcggccctcg acgaagccgg catcgcggcc ctcggcgtgg ccttcggcgt
     1201 aacccttggc gcggccctcg gcgcggccgg cggcatagcc ttcctcgcgc gcggcacgct
     1261 cgatcgcctc gatctcctcc agcgtcggca gccgcggcat cgggatcgac ggctccggct
     1321 ccggcagcgg ggcgccggcc gacttgccgg gcggtgcggc caccggttcg ggcgcggcct
     1381 cgggctgggc cttgtcgaag ccggggaact gccagcgcgg caggtcacgt ccggcctgcc
     1441 ggggaatgat ccggtcagac atagccacca ccgccactgc cgaggttgag gtcgccggct
     1501 tcggccatgc gcttggcgat cgtcatgatg tccttctgcg cagcctcgac atcggcgagg
     1561 cgcaccggcc ccatcgattc gaggtcgtcg cgcatcatct cggcggcgcg cttggacatg
     1621 ttgcgcagga tcttctcgcg gatcgtctcg tcggcgccgc gcatcgcgac gatcagggtt
     1681 tccgaggaca cctcgcgcag caggttctgg atgccgcggt cgtcggcgtt gagcaggtgc
     1741 tcgaacacga ccatcagatc ctcgatgcgc agcgccagcg actcgtccat gcccttgatc
     1801 ttgtccatca gcggcgtgct gatgccggtg tcgatggcgt tgaggatttc ggcggcgcgc
     1861 ttcggaccgc cgaccttgaa gctgctggcg gcgttgatgt tgcgcatcac ctgcttctcg
     1921 atgacctcgt tcagctcgcc gatcgccgcg gtcggcaccg agtcgagcgt cgcgacgcgg
     1981 atcagcacgt cgtggcgcag cgtctcgggc agcagcgcca gcacctcggc ggcctgcgcc
     2041 gattcgaggt aggacagcac cagcgcgatg atctgcggat gctcgcggcg caggccggcc
     2101 gcaaccgcct gcgcctccat ccacttcagc gcctcgacgc cggtggcgcc ggcctcgttg
     2161 aggatgcggt ccatgacgtt cttggccttg tcacggccga gggcgcgcgt cagcacgttc
     2221 ttcatgaagt cgcggctgtc gatgacgagg gcgttctggt tgacgagttc gttgttgaac
     2281 tcggcgacga ccgtcgacac cgcatcgcgc ccgaccgagg acaggctcgc catcgaggcg
     2341 ccgatgcggt gcagctcgcg cggatcgaac tgcttgagca gctcggcggc gtactcctcg
     2401 ccgagcgcca tcatcagcac cgcgacgcga tccgggccgc tcagggcttc accggccatt
     2461 tcactcatgg ttcaggcctc ctcctcttcc tgcgacgcca gcagtgccat gccctcgccg
     2521 ggctgacggt tcatccacgt gcgcagcacg ctgacggcgc ggcgcgggtc ctcggccacc
     2581 agccggcggg cgatgtcgag tcgttcttcg agccgctcga tggcctcggc gtcatcgggc
     2641 tgcggcagcg gcaccggcgc cttgccgttg ccgttgccgt gatgcccgtc ggcctgcgcc
     2701 agcgcctggc cggcggccga cagctgcacg gcatcggcat cctcgtcgcc gccggcagcg
     2761 gcctcatcgc cgctcgcctc gccgccttcg gcaggcacca gtgccttgcc gccggcatcg
     2821 gtggtcgcga gcgccgtgcc cggtccggtt tcggtggcgg ccggagccgc gagcgcagcg
     2881 gcggccgccg gttccggtgc cggcagcgcc ggcggcggag gcagcaggcg gcgcacgacc
     2941 ggtcgcacga cgacgagcag gaacacgatc gcgacgatgg cgaccaggct ccacttgacg
     3001 agttcgatga accagtcctg acgccagaac ggcagcaggt tcggatcgaa gggagtggtc
     3061 gcgaaggtcg cgttcatgac gttcaccgtg tcgccccgct gggcgtcgaa tcccaccgct
     3121 tcgcgcacga gcgcggtgat gcggtcgatg tcctcctgcg gccacgaaac ccgctcgacc
     3181 gagccttcgg gccgcaccgt gatgcggtca tcgagcacga ccgcgaccga cacgcgccgc
     3241 acgcgccccg gtgcagcggt gatgtgactg acgcgcttgt cgagctcgta gttgcgcgtg
     3301 ctctggctgc gcgtgcgcat cgggcggctg ccgtcggcgg cgcgctgcgc gtcgctctgc
     3361 tgggcgccgg ccgcggcagc ggcctggccc tgcggcccga tctggccgtt gccggtctgg
     3421 ccaccgccgg gctgcggcgc attcggattg ttcggatcgg cctgcgcggc cggcgcgccg
     3481 gcggcctgct cgggcgcggt cgcgaccccg ggcggctggt tcgacagcgc accgggaatg
     3541 cccatcggct gcgggcggtt gttgttctcc tcgatcagct cctcgctgcg caccatgccg
     3601 cgctcgcgct tcggatcgaa ggattcggag gtctcctcca gctgcgagaa gtccagatcg
     3661 agcgccacct gcgcgcgcat cttgttggcg ccgaggatcg gcgtcagcag gtcctcgatg
     3721 cggcgcgtgt agttcgattc cagccgctgc tggtattcca tctggtcgag gttcaggccc
     3781 ggcgtgccgc cggccgggcg cgtcagcagg ttgccggcct gatcgacgac cgagacctgc
     3841 gagggcgtca gccgcggcac gctcgaggac acgaggtgca cgatcgccgc gacctggccg
     3901 gcgtcgatcg cccgccccgg ctgcagctgc accagcaccg aggcggtcgg ctgctgcttg
     3961 ttgcgcacga acaccgtgtc cttcggcagc gccagatgca cgcgggcgtt ggcgacggcg
     4021 ccgatgctca tgatcgagcg cgccagctcg ccctcgagcg cgtgctggta gcgggcgttc
     4081 tccatgaact ggctggtgcc gaagcccgac tgctgctcga aggtgtcgaa gccgccggtc
     4141 gtgccgcgcg gcaggccctg cgcggcgagc ttcaggcgca gctcggcgac gcgatcggcc
     4201 ggcacgttca gcgtgccggt ctggcgatcg acgcgcaccg gcacctgcat cttgttcagg
     4261 gcttccatga tctggccggc gtcggcctcg gccagaccgt ggtacagcgg ctggaaatcg
     4321 gggcggccgg ccgacagcgc cacggccacg atggtcgcga gcagcagcac gccgagcgcg
     4381 atcgcgccgt acatccacgg ccccggcgtg cgcacgcgca ccagccagtc gcgccagccg
     4441 gcgcgcgccg gcgcggcatc ggcggtgtcc gtggcagtgg cgagcgaggt gtcttcggtc
     4501 acgacggtgt tcctcggtat tcctcaaggc gctcctgaca gggaatccgc cggctcagag
     4561 cggcatgttc aggatgtcct gataggcggc gaccagcttg ttgcgcacct gcagcgccga
     4621 ctggaagctc agccgcgact gctgcatcgc gatcatcacg tcgggcaggt cggcgccctc
     4681 ctcgccgagc tcgaaggccc gcgccatgcg cgaggcctgc tgctggcggg cgtcgacgcg
     4741 atcgagcgcg cccttgagca ggctggcgaa atcggcatcg ccggcaccgg cttcagggcc
     4801 ggcagcggct ccggtgtcgg tggcggcagc gcgcagggcg gcgatcgagg cgttcagcgg
     4861 ggacagggtc atgagcttcg gtctcctgga ttcggtatca ggccggatca ggccgggtcg
     4921 tgtcgggcgt acgcgggcgg ggcgcggccg gttcaggcca cgcgctggcc gccggcaggc
     4981 gggccgttgc cggcggcagc ggcggggatc ccggcctccg cggccgggtc acggggaaga
     5041 tcgagcgccg gcagacgcag cgtcagccgg ccatcg
//
LOCUS       DAOQJH010000088         5054 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_9726_length_5054_cov_4.666933, whole genome shotgun sequence.
ACCESSION   DAOQJH010000088 DAOQJH010000000
VERSION     DAOQJH010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 5054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5054
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9726_length_5054_cov_4.666933"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1213)
                     /gene="ccsB"
                     /locus_tag="PLQ18_03210"
     CDS             complement(<1..1213)
                     /gene="ccsB"
                     /locus_tag="PLQ18_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018747146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HNE00690.1"
                     /translation="MAVTRERMLDVLGNGPESAPRRPATAEWIWIALVLTGAGWAGWT
                     YGAVMDGYELAILAGSALALIAWGWHWRPARAYSAAVAALSLFAIGRYGDSLGAAQTD
                     FFLKYLFASQQAFMWMSALYVLATVTYAAALVSRSDFVGRTASALTWTASVMGLAGLM
                     VRWRESYLMGADIGHIPVSNLYEVFILFSVITALLYLYYEGRTRTRAMGAFVLLVISA
                     AVGFLLWYTFDREAQHIQPLVPALQSYWMKIHVPANFIGYGAFSLAAMLGVVYLLAQP
                     QQAAPALRRVLWPAGALLVATGAAMVGWTFTHEEAAKVLRAWALLPVTAGLLVWVVAG
                     QGRMADRLPAPAVVDNILYKSIALGFAAFTVATILGALWAAEAWGGYWSWDPKETWAL
                     IVWLNYAAWLHL"
     gene            complement(1224..3263)
                     /locus_tag="PLQ18_03215"
     CDS             complement(1224..3263)
                     /locus_tag="PLQ18_03215"
                     /inference="COORDINATES: protein motif:HMM:NF016995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HNE00691.1"
                     /translation="MTAVRSARAARPALLSEFLGSMNLAITLLVVVAVASVIGTVLKQ
                     NEPYTNYVIKFGPFWFEVFRKLGLYDVYGAPWFLAILGFLVLSTGVCLWRHVPGMLAE
                     INHFRLTVQAKSLRAFHSHAEWTLAADPATVAAQAAGALRAQGYRVRSSTHEDGHTVL
                     AALRGSLNRFGYLFTHLAIVVICVGGLLDGNLPLKWKEWRGEVKLETRDVVARDVPPE
                     SRLAPGDSLSFRGNVRLPEDSSANFVFLRLRDGFFVQELPFAIELKQFRVEFYPTGQP
                     KNFESDVLIHDADRLKTPLASTIKVNHPLVYRGYAIYQSDFGDGGSLMRMTAQPLLGQ
                     SAAPPKLEGRIGEKLSLDTPQGPLTLELDDFRLYNILPVPASEQQAASDAVGEPGRKV
                     RNFGPNFTYRLRNAAGQALEFQNFMQPFRLDGRSFFLSGVRGTPSEEFSYLHIPADAQ
                     GTPGRFLRFTALLHDRARVQAVLARAGTTAPGMSAAELAAVRLRLVDLFLKQGIDGVI
                     EQTRQRVPAERAEEATRFFLEVLRMSLGELYVELLRGEGLAADAEPGPADEQFFNDAL
                     NALSAIPNYGAPFWLQLDGFEQVQASGLQITKAGGQNVVYLGFALLTAGVFVMFYTSH
                     RRVWVWLAPEDGGTRLILAGSGNRHQEAFVQEFERLEAAVAVQTGAATLPPNPPS"
     gene            complement(3392..4000)
                     /locus_tag="PLQ18_03220"
     CDS             complement(3392..4000)
                     /locus_tag="PLQ18_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018233361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNE00692.1"
                     /translation="MKKLFLIAAASALIGSAPLALAAGDAAAGQAKSAMCGACHGADG
                     NSASADFPKLAGQNAGYLYKQLAEFKSGARVNPTMQGMVAALSDEDMQNLAAYFASQT
                     PGKGEADPALVKAGEALFRGGNLDTGVSACSACHGVSGKGNEGAKFPALAGQHAKYTE
                     VQLLAFRSMARANDPGQMMRGVAARMSDAEIKAIASYLQGLQ"
     gene            4218..4832
                     /gene="yihA"
                     /locus_tag="PLQ18_03225"
     CDS             4218..4832
                     /gene="yihA"
                     /locus_tag="PLQ18_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011630590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HNE00693.1"
                     /translation="MNERYRSAHFLKSANELHQLPPDEGIEVALAGRSNAGKSSALNT
                     LTGQSRLARVSKTPGRTQLINLFELDAGRRLVDLPGYGYAQVPEAVKRHWQAVLARYL
                     EQRQALAGLLLVMDVRHPFNPLDRQMLDWTCHRGLATHVLLTKSDKLSRGAAASTLAQ
                     VRRELAAQYPNAGAQLFSSLDRSGLDAAHARLDAWFGFGDAAGS"
BASE COUNT          805 a         1779 c         1755 g          715 t
ORIGIN      
        1 gcaggtgcag ccaggcggcg tagttcagcc agacgatcag cgcccaggtt tccttcggat
       61 cccaggacca gtagccgccc caggcctcgg ccgcccacag cgcgccgagg atggtcgcga
      121 ccgtgaacgc cgcgaagccg agcgcgatcg acttgtagag gatgttgtcg accacggccg
      181 gtgccggcag ccggtcggcc atgcgcccct gaccggcgac cacccagacc agcagcccgg
      241 ccgtgaccgg cagcagcgcc caggcgcgca gcaccttcgc ggcttcctca tgcgtgaagg
      301 tccagccgac catcgccgcg ccggtggcga cgagcagcgc gcccgccggc cacagcacgc
      361 ggcgcagcgc cggtgcggcc tgctgcggct gcgcgagcag atagacgacg ccgagcatcg
      421 ccgccagcga gaaggcgccg tagccgatga agttggccgg cacgtggatc ttcatccagt
      481 agctctgcag cgccggcacc agcggctgga tgtgctgggc ctcgcgatcg aaggtgtacc
      541 agagcaggaa gccgaccgcg gccgagatca ccagcagcac gaaggccccc atcgcccggg
      601 tgcgcgtgcg gccctcgtaa tagaggtaca gcagcgccgt gatcaccgag aacaggatga
      661 agacttcgta gaggttgctg accgggatgt ggccgatgtc ggcgcccatc aggtaggact
      721 cgcgccagcg caccatcagc ccggccagcc ccatgacgct cgccgtccag gtcagcgcgc
      781 tggcggtgcg cccgacgaag tcggagcgcg acaccagcgc cgccgcgtag gtgacggttg
      841 ccagcacgta cagcgcgctc atccacatga acgcctgctg gctcgcgaac aggtacttca
      901 ggaagaagtc ggtctgtgcg gccccgaggc tgtcgccgta gcggccgatc gcgaacagcg
      961 acagcgccgc gaccgcggcc gagtacgcgc gcgccggccg ccagtgccag ccccacgcga
     1021 tcagcgccag cgccgagccg gcgaggatcg cgagttcgta gccgtccatg accgcgccgt
     1081 aggtccagcc ggcccagccg gcgccggtca gcaccagcgc gatccagatc cattccgccg
     1141 tggccggccg gcgcggggcg ctttccgggc cattgccgag cacgtcgagc atgcgctcgc
     1201 gggtcaccgc catgatcgtc acctcaggat ggaggattcg gggggagcgt cgcggcaccg
     1261 gtctgcacgg cgaccgcggc ttcgagccgt tcgaactcct gtacgaaggc ttcctgatgg
     1321 cggttgccgc tgccggcgag gatcagccgg gtgccgccat cctcgggcgc gagccagacc
     1381 cagacgcgcc ggtgcgaggt gtagaacatc acgaacacgc cggccgtcag cagcgcgaag
     1441 ccgaggtaaa ccacattctg gccaccggct ttcgtgatct gcaagccgga agcctgcacc
     1501 tgttcgaagc catcgagctg cagccagaac ggcgccccgt agttcgggat cgccgacagc
     1561 gcgttcagcg catcgttgaa gaactgctcg tccgcggggc cgggctcggc atcggcggcc
     1621 agaccctcgc cgcgcagcag ctcgacgtac agctcgccga ggctcatgcg cagcacttcg
     1681 aggaagaagc gcgtcgcctc ctcggcacgt tcggccggca cgcgctggcg ggtctgctcg
     1741 atgacgccgt cgatgccctg cttcaggaac agatcgacga ggcgcaggcg caccgccgcc
     1801 agttcggccg ccgacatgcc aggcgccgtc gtgccggcgc gcgcgagcac ggcctgcacg
     1861 cgggcgcggt cgtgcagcag cgcggtgaag cgcaggaagc gccctggcgt gccctgcgca
     1921 tcggccggga tgtgcaggta gctgaactcc tcggacggcg tgccgcgcac gccggacagg
     1981 aagaacgagc gcccgtcgag gcggaacggc tgcatgaagt tctggaactc cagcgcctga
     2041 ccggcggcgt tgcgcagccg gtacgtgaaa ttcggcccga agttgcgcac cttgcgcccc
     2101 ggctcgccga ccgcgtcgct cgcggcctgc tgctcggagg ccggcaccgg caggatgttg
     2161 tagagccgga agtcgtcgag ttcgagcgtc agcggcccct gcggcgtgtc gagcgagagc
     2221 ttctcgccga tgcggccttc gagcttcggc ggcgcggcac tctggccgag cagcggctgc
     2281 gcggtcatgc gcatcagcga gccgccgtcg ccgaagtcgg actggtagat cgcgtagccg
     2341 cggtagacca gcgggtggtt gaccttgatc gtcgaggcga gcggtgtctt cagccggtcg
     2401 gcatcgtgga tcagcacgtc gctctcgaag ttcttcggct ggccggtcgg atagaactcg
     2461 acgcggaact gcttcagctc gatcgcaaac ggcagctcct gcacgaagaa gccgtcgcgc
     2521 aggcgcagga acacgaagtt cgccgagctg tcctcgggca ggcgcacgtt gccgcgaaac
     2581 gacaggctgt cgccgggcgc gaggcggctc tccggcggca cgtcgcgcgc gaccacgtcg
     2641 cgggtttcga gcttcacctc gccgcgccat tccttccatt tcagcggcag gttgccgtcg
     2701 agcaggccgc cgacgcagat cacgacgatc gccagatgcg tgaacaggta gccgaagcgg
     2761 ttgaggctgc cgcgcagcgc cgcgagcacg gtgtggccgt cctcgtgcgt gctgctgcgc
     2821 acgcgatagc cctgggcgcg caacgcgcca gccgcctgcg cggcgaccgt cgccggatcg
     2881 gcggcgagcg tccattcggc gtggctgtgg aaggcgcgca gcgacttcgc ctgcaccgtc
     2941 agccggaagt ggttgatctc ggcgagcatg cccggcacgt gccgccacag gcagacgccg
     3001 gtcgacagca ccaggaagcc gaggatcgcc agaaaccacg gtgcaccgta gacatcgtac
     3061 aggccgagct tgcggaacac ctcgaaccag aacggtccga acttgatcac gtagttcgtg
     3121 tacggctcgt tctgcttcag taccgtgccg atcaccgaag ccaccgcgac caccaccagc
     3181 agcgtgatcg cgaggttcat cgagccgagg aattcggaca gcagcgccgg acgggcggcg
     3241 cgggcggaac ggacggcagt catcgggcgc tcgatgcggg cggtccgtca ccggaccggg
     3301 agaaaagcga aagggtggcg ccgggccacc cttgttgcac gtatcgcacg taactgcgtc
     3361 ggcggactcc cggcaggcgg gagagccggg cttactgcag gccctgcagg tacgaggcga
     3421 tggccttgat ctcggcgtcg ctcatgcgcg ccgcaacgcc gcgcatcatc tggcccggat
     3481 cgttggcgcg ggccatcgag cggaaggcga gcagctggac ctcggtgtac ttggcgtgct
     3541 ggccggccag cgccgggaac ttcgcacctt cgttgccctt gccgctgacg ccgtggcagg
     3601 cggagcaggc cgagacgccg gtgtcgaggt tgccgccgcg gaacagcgcc tcgccggcct
     3661 tcaccagcgc cggatccgcc tcgcccttgc ccggagtctg gctggcgaag taggccgcga
     3721 ggttctgcat gtcctcgtcg ctcagcgcgg caaccatgcc ctgcatggtc gggttgacac
     3781 gcgcgccgga cttgaactcg gccagctgct tgtacaggta gccggcattc tggcccgcga
     3841 gcttcggaaa gtccgcgctc gcgctgttgc cgtcagcacc gtggcaggca ccgcacatcg
     3901 cggacttcgc ctgaccggca gcggcgtcgc ccgcggccag ggccagcggg gccgagccga
     3961 tgagggcgct cgcagcggca atcaagaaca gcttcttcat gttgaaacga ctcctgtacg
     4021 atgcgggatt cggtgttctg aaacccggcg gcccgcccct ggaaacaggc ttcgcgccgg
     4081 gctctctccc ctcccccggc gggcacgcaa ggggcgaaaa cagccgcgct atataccagt
     4141 tccggcaggg tcattcaatc gcaagccccg tcacagcggt gctttgccgg tcgatcgccg
     4201 ccggcggatt ccggaccatg aacgagcgtt accgcagtgc gcacttcctc aagagcgcca
     4261 atgaactgca ccagctgccg cccgacgagg gcatcgaggt ggcgctggcc gggcgttcca
     4321 acgccggcaa gtcgagcgcg ctgaacacgc tgaccggcca gtcgcggctc gcgcgcgtca
     4381 gcaagacgcc cgggcgcacg cagctgatca acctgttcga gctcgatgcc gggcgccggc
     4441 tggtcgatct gcccggctac ggctacgcgc aggtgcccga ggccgtgaag cgccactggc
     4501 aggcggtgct cgcgcgctat ctggagcagc gccaggcgct ggccgggctg ctgctggtca
     4561 tggacgtgcg ccacccgttc aacccgctcg accggcagat gctcgactgg acttgccacc
     4621 gcggccttgc gacgcacgtg ctgctgacga agtccgacaa gctctcgcgc ggggccgcag
     4681 cctcgacgct cgcgcaggtg cgccgtgagc tcgccgcgca gtacccgaac gccggcgcgc
     4741 agctgttctc gtcgctcgat cgcagcggcc tcgatgccgc gcacgcccgg ctcgacgcct
     4801 ggttcggctt cggcgacgcg gccgggagct gaaagccggg cgcgggctgc gccagcctcc
     4861 ggccctcctt cgccctgccc tgcgcgggct gcggcccggc ctcagccgcg cgccttcagc
     4921 caggcctcga agcgcccggc gtccatcggc cgcgccagca ggccgccctg gtattcctcg
     4981 cagccggcgg ccttcagcac gtcgagctgg ccgcgctgtt cgacgccctc ggccagcgtg
     5041 ccgagcccga gttc
//
LOCUS       DAOQJH010000089         5051 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_9737_length_5051_cov_3.575661, whole genome shotgun sequence.
ACCESSION   DAOQJH010000089 DAOQJH010000000
VERSION     DAOQJH010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 5051)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5051)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5051
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9737_length_5051_cov_3.575661"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..577
                     /locus_tag="PLQ18_03230"
     CDS             <1..577
                     /locus_tag="PLQ18_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - glucose binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HNE00694.1"
                     /translation="AGTAIGLIGPGTGLGVSGLLPDGHGGWRAIEGEGGHVSFSPADA
                     LELEILRWALARHPHVSVERLVSGALGLPNLHQALAEIDGLPAEAMAAPEIVQRGTSG
                     DCALCRRVLTVFCAMLGTAAGNLALTLGARGGVYIGGGIVPRFPEFFAASPFRARFEH
                     RGRFTTYLQPIPCFVIHAPDAALRGAAAALD"
     gene            complement(587..1357)
                     /locus_tag="PLQ18_03235"
     CDS             complement(587..1357)
                     /locus_tag="PLQ18_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006579247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNE00695.1"
                     /translation="MPILHTDFLLPALALGVALGWLGGLFGIGGGLIAIPVLGLACGL
                     DQLHAQGTALVMVLPNVVIGFWRYRARHGIAWRPALLLGLTAIAGSEFAAHLATGLPA
                     VTLRLMFAGFLIVLAGWLLRGLRRAPPATAAPAPAVLSARWLPAVGLAGGLMSGLFSV
                     GGGIAAVPLLTGLFGTRQAVAQGLALALVTPGSAAALFAYARAGAVDWPLGLALAFGG
                     VLSVSAGVAFAHRLPERRLRALFALGIVATAGAMLASA"
     gene            1591..2496
                     /locus_tag="PLQ18_03240"
     CDS             1591..2496
                     /locus_tag="PLQ18_03240"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNE00696.1"
                     /translation="MELAWLEDFLAVIDCGSFSRAADSRHVTQPAFSRRIRSLEDWIG
                     TPLFERSSHGAALTAAGERFRPSAEAALRKLLHGREDAIAAADSGLPLLRFASTHALA
                     LDFFPQWLRKVEARSPVDASIRLIADTMHACEQLLLRGEAQFLLCHGHDGVSGRLDNG
                     DYRSITVGNDVLLPVSAPRAANDPRPRFELPGSPDEPLPLLAPTEASGLSRALAAAQA
                     QLAPAQVRPAFTAHLATVLAAMARDGRGLTWVPQRLVADDLAAGRLVHAGDASWTIPM
                     SIRLYRPRNLTGAAATRLWAAAGGD"
     gene            2560..2979
                     /locus_tag="PLQ18_03245"
     CDS             2560..2979
                     /locus_tag="PLQ18_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNE00697.1"
                     /translation="MTETASTSAGDRAAVVTVRPEAEVLTRQRLPYFVGICAATAGAR
                     GLAMHLVVIPPGAASEPHLHRGYETAIYILEGRVETRYGRRLEHRVVNVAGDFVYIPA
                     DLPHQAVNLSATDPARALVARNDAADREHVDPWQAPV"
     gene            3232..>5051
                     /locus_tag="PLQ18_03250"
     CDS             3232..>5051
                     /locus_tag="PLQ18_03250"
                     /inference="COORDINATES: protein motif:HMM:NF017081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNE00698.1"
                     /translation="MLLTGLGCFAAAVICILLSRGSSPLATIWLPNAILLAVALRAPY
                     RQIWPVVITGAAAGLCANLATDVSPGLAAGYAFANLVSVLVPLLALRPADSQPFLLTE
                     RPTSAVRLILVVATGTMLSALVGGALLATVHARPLPASIGNWLTSDLTGMVAVLPLGL
                     RASLRESHYWFAAGRRTEILLLYGLTIAGTFASLQLSPFPFVTIELPLLLASFRLGLG
                     GTALLCACESLLFAALLSVLPPVPMQMALQTYGPGINALAQFAPLAVAWLLECRSADM
                     GRLRASEERLRSAMRDSAIGMALIDLEGRFVAVNPALCALGGHEAQALLGHHFADFIH
                     GPDRYVAQELARQLAEGTIGSTRYELRGLRADGSLHWLRVVASCMRDGQQRPTGFIAQ
                     IEDIDAQRQAERARAEQAALIERAHDAIIGRDAEGRVTHWNRGAERLYGWSAAEMTGQ
                     PLHERLDTHNAVSGAADDPERTALDYWEGTLLQRHRDGHALTIESRRVLLRGPDGRLS
                     ATLEINRDVSTLREAVHLHAQFVALVARELRAPLTTIGGTLDLIASGALGTVSGEAQD
                     LIDIARRHDARLMRIVADIQDAERIAAGDVPLAPRTIALAA"
BASE COUNT          690 a         1928 c         1735 g          698 t
ORIGIN      
        1 cgcgggcacg gcgatcgggc tgatcggccc cggcaccggg ctcggggtgt ccggactgct
       61 gccggacggg cacggcggct ggcgcgcgat cgagggcgag ggcgggcacg tgagcttcag
      121 cccggccgat gcgctggagc ttgaaatcct gcgctgggcg ctcgcccgcc acccgcacgt
      181 gtcggtcgag cggctggtct ccggcgcgct cggcctgccg aacctgcatc aggccctcgc
      241 cgagatcgac ggcctgccgg ccgaggcgat ggcggcgccc gagatcgtgc agcgcggcac
      301 cagcggcgac tgcgcgctgt gccgacgggt gctgacggtg ttctgcgcca tgctcggcac
      361 cgcggccggc aatctggcgc tgacgctcgg cgcgcgcggc ggcgtctaca tcggcggcgg
      421 catcgtgccg cgcttcccgg agttcttcgc cgcctcgccg ttccgggcgc gcttcgagca
      481 tcgcggccgg ttcacgacct atctgcagcc gatcccgtgc ttcgtgatcc atgcccccga
      541 tgcggcgctg cgcggcgcag cggcagcact cgactgaacg accgcttcag gccgatgcga
      601 gcatcgcgcc ggcggtcgcg acgatgccga gcgcgaacag cgcacgcagc cgccgctccg
      661 gcagccggtg cgcgaatgca accccggccg acacgctcag cacaccgccg aaggccagcg
      721 ccagcccgag cggccagtcg acggcgccgg cacgggcata ggcgaacagc gccgcggccg
      781 agccgggcgt gaccagcgcc agcgccaggc cttgcgcgac cgcctgacgc gtgccgaaca
      841 gcccggtcag cagcggcacc gcggcgatgc cgccgccgac gctgaacagt cccgacatca
      901 gcccgccggc cagtccgacc gccggcagcc agcgcgccga cagcacggcc ggtgccggtg
      961 ccgccgtggc cggcggcgcg cggcgcagcc cgcgcagcag ccagccggcc agcacgatca
     1021 ggaagcctgc gaacatcagg cgcagcgtca ccgccggcag gccggtggcg aggtgtgcgg
     1081 cgaactcgct gccggcgatc gccgtcagcc cgagcagcag cgccggtcgc caggcgatgc
     1141 cgtgtcgcgc ccggtagcgc cagaagccga tcacgacatt cggcagcacc atcaccagcg
     1201 ccgtgccctg cgcgtgcaac tggtcgagcc cgcaggcgag tccgagcacc gggatcgcga
     1261 tcaggccgcc gccgataccg aacagaccgc cgagccagcc gagtgcgaca ccgagtgcga
     1321 gcgcgggcag cagaaagtcc gtatgcagaa tcggcatgtt cgtctccgga cgggcaagcg
     1381 aggagtgcaa gccagcgcaa gcagcgctac cgggcctgac ccctgaattc tcgctggata
     1441 cgcggatttg cagccaatgc cgatcgcgca ggtggccatg ccgaaaaaac atggccttgt
     1501 cactacgacg tttgccgtat gccaatgtcg cactgcaata aggcaggctg cacgcaggaa
     1561 ttcactggtg cgtcaggagt acataagccc atggaactcg cctggcttga ggattttctc
     1621 gccgtcatcg actgcggcag cttctcgcgt gccgccgaca gccggcacgt cacgcagccg
     1681 gccttcagcc gccgcatccg ctcgctggag gactggatcg gcacgccgct gttcgagcgc
     1741 agcagccacg gcgcggcgct gaccgcagcc ggcgaacgct tccggccctc ggccgaggct
     1801 gcgctgcgca agctcctgca cgggcgcgag gatgccatcg ctgccgccga cagcggcctg
     1861 ccgctgctgc gcttcgcgag cacgcatgcg ctggcgctgg acttcttccc gcagtggctg
     1921 cgcaaggtcg aagcccgcag ccccgtcgat gcctcgatcc gcctgatcgc cgacacgatg
     1981 cacgcctgcg agcagttgct gctgcgcggc gaggcgcagt tcctgctctg ccacggccac
     2041 gacggggtca gcggccgcct cgacaacggc gattaccgct cgatcacggt cggtaacgat
     2101 gtgctgctgc cggtgagcgc tccgcgtgca gccaacgatc cgcgcccgcg cttcgagctg
     2161 cccggctcgc cggacgaacc gctgccgctg ctggcaccga ccgaagcctc gggcctgagc
     2221 cgggcgctcg ccgccgcgca ggcgcagctc gcgccggcgc aggtgcgccc ggccttcacc
     2281 gcgcatctgg cgacggtgct ggcggcaatg gcgcgcgacg ggcgcggcct gacctgggtg
     2341 ccgcagcgcc tggtcgccga cgatctcgcg gccggccggc tggtgcacgc cggcgatgcg
     2401 tcctggacca tcccgatgtc gatccggctc taccggccgc gcaacctcac cggtgccgcc
     2461 gccacgcggc tgtgggccgc cgccggcggc gactgaggca gccgcgcgcg ccggcagcgg
     2521 ctacgctcgc ggtcgatgtc cgcgccggag gccgccacca tgaccgaaac cgcatccacg
     2581 agcgccggcg accgtgccgc cgtcgtcacc gtccggcccg aggccgaagt cctgacccgc
     2641 cagcgcctgc cgtatttcgt cggcatctgc gcggccaccg ccggcgcccg cggcctggcg
     2701 atgcatctgg tggtgatccc gccgggcgcg gcttccgagc cgcacctgca tcgcggctac
     2761 gagacggcga tctacattct cgaaggccgc gtcgagaccc gctacggccg gcggctcgaa
     2821 caccgggtcg tcaacgtcgc cggcgacttc gtctacatcc ccgccgacct gccgcaccag
     2881 gccgtcaatc tgagcgccac cgaccccgcc cgggcactgg tggcccgcaa cgacgcagcc
     2941 gaccgcgaac acgtggatcc ctggcaagcg cctgtctgac caaggcgtca tgttcacggc
     3001 gttccctcgc tgcgccggac tgtggtaaac gggcttcccg ccctggctgc gtcgcctcat
     3061 gccagctccg cattccggcc cggtccgctg ctgcagacgc ttccgcaccc cgaccgtgcc
     3121 agctcccgac cggcctcgcc gccgccccga gaatccgtgg ccctcatgac cgatcccctc
     3181 acgtccgtac cggctgacgc tcccccgtca ccgatgccga cggggttgcg gatgctgctg
     3241 accggtctcg gctgtttcgc ggcggccgtg atctgcatcc tgctgtcgcg cggcagcagc
     3301 ccgctcgcca cgatctggct gccgaacgcc atcctgctcg ccgtcgcgct gcgggcaccg
     3361 taccggcaga tctggccggt ggtgatcacc ggagccgccg ccggcctgtg tgcgaacctg
     3421 gccaccgacg tctcgcccgg tctggccgcc ggctatgcgt tcgcgaatct ggtcagcgtg
     3481 ctggtgccgc tgctcgcgct gcgcccggcc gattcgcagc ccttcctgct gaccgagcgc
     3541 ccgacaagcg ccgtgcggct gatcctcgtg gtcgccaccg gcacgatgct gtcggcgctc
     3601 gtcggcggcg cgctgctggc gaccgtgcac gcacggccgc tgccggcctc gatcggcaac
     3661 tggctgacca gtgacctgac cggcatggtc gcggtgctgc cgctcgggct gcgcgccagc
     3721 ctgcgcgagt cgcactactg gttcgcggcc gggcggcgca ccgagatcct cctgctgtac
     3781 gggctgacca tcgccggcac cttcgccagc ctgcagctca gtccgttccc gttcgtgacc
     3841 atcgaacttc cgctgctgct cgcctcgttc cggctcggcc tcggcggcac ggcgctgctg
     3901 tgtgcctgcg agagcctgct gttcgccgcc ctgctgtcgg tactgccgcc ggtcccgatg
     3961 cagatggccc tgcagaccta cgggccgggc atcaatgcgc tggcgcagtt tgcaccgctc
     4021 gccgtcgcct ggctgctgga atgccgctct gccgacatgg gccggctgcg cgccagcgag
     4081 gagcgcctgc gcagcgcgat gcgcgattcg gcgatcggca tggcgctgat cgatctcgaa
     4141 ggccgcttcg tcgccgtgaa cccggcgctc tgcgcgctcg gcggccacga ggcgcaggcc
     4201 ctgctcggcc atcacttcgc cgacttcatc cacggcccgg accgctacgt ggcgcaggag
     4261 ctcgcgcggc agctcgccga gggcacgatc ggttcgaccc gctacgaact gcgcggcctg
     4321 cgcgccgatg gctcgctgca ctggctgcgc gtggtcgcct cgtgcatgcg cgacggccag
     4381 cagcgcccga ccggcttcat cgcccagatc gaggacatcg acgcccagcg tcaggccgaa
     4441 cgcgcgcgcg ccgagcaggc ggccctgatc gagcgcgcgc acgacgcgat catcggccgc
     4501 gatgccgagg gccgggtcac gcactggaac cgcggcgccg agcgcctgta cggctggagc
     4561 gcggccgaga tgaccggcca gccgctgcac gagcgcctgg acacgcataa cgccgtcagt
     4621 ggcgcggccg atgatccgga gcgcaccgcg ctcgattact gggaaggcac gctgctgcag
     4681 cgtcaccgtg acggtcacgc gctgacgatc gagagccgcc gcgtgctgct gcgcggcccc
     4741 gacggccggc tgtcggcgac gctggagatc aaccgcgacg tgagcacgct gcgcgaggcc
     4801 gtgcatctgc acgcgcagtt cgtggcgctg gtggcgcgcg agctgcgcgc gccgctgacc
     4861 acgatcggcg gcacgctcga cctgatcgcc agcggcgcgc tcggcacggt ctccggcgaa
     4921 gcgcaggatc tcatcgacat cgcccgccgc cacgatgccc ggctgatgcg catcgtcgcc
     4981 gacatccagg acgccgaacg catcgccgcc ggcgacgtgc cgctggcccc gcggacgatc
     5041 gcgctcgctg c
//
LOCUS       DAOQJH010000090         5040 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_9765_length_5040_cov_3.894885, whole genome shotgun sequence.
ACCESSION   DAOQJH010000090 DAOQJH010000000
VERSION     DAOQJH010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 5040)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5040)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5040
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9765_length_5040_cov_3.894885"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..971)
                     /locus_tag="PLQ18_03255"
     CDS             complement(<1..971)
                     /locus_tag="PLQ18_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007640625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HNE00699.1"
                     /translation="MTHPAFEHLRSRAVPALKLELQEYRHRATGARHVHLASDDPHKA
                     FLVAFLTVPQDSTGVAHILEHTALCGSRRYPVRDPFFMMTRRSLNTFMNAFTGADWTA
                     YPFASLNRKDFENLLDVYLDAAFFPLLDPLDFAQEGHRVEFADDSADAPLVFKGVVYN
                     EMKGAMSSPVQALAQRLQSALFPTTTYHHNSGGEPGVIPDLTHERLVAFHGAHYHPSN
                     AVFMSYGDLPAAELQQHFEERALSAFTARPLDLAIADERRYDAPVAVEAPYAADAEAL
                     ADGTHVVLGWLLGRTTDPLETARARVLSDVLLDNSSSPLRHALETSEL"
     gene            complement(1360..1584)
                     /locus_tag="PLQ18_03260"
     CDS             complement(1360..1584)
                     /locus_tag="PLQ18_03260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE00700.1"
                     /translation="MWEFVDWETAMQATCTCGGQICSCPKCYRRIQSLHARRWEAAQL
                     GLPNGVVLLGWNAYDGAQFTDLRREPLKHD"
     gene            complement(1832..2146)
                     /locus_tag="PLQ18_03265"
     CDS             complement(1832..2146)
                     /locus_tag="PLQ18_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004368410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNE00701.1"
                     /translation="MKAIHGIAGALLLGAIVAAPAQADGLALAQKNNCLACHAVDQKL
                     VGPSYKEVAAKYKGDATAPDKLAAKVKAGGAGSFGQIPMPPNPQVSDADMKEIIAWIL
                     AL"
     gene            complement(2458..3783)
                     /gene="argA"
                     /locus_tag="PLQ18_03270"
     CDS             complement(2458..3783)
                     /gene="argA"
                     /locus_tag="PLQ18_03270"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007192071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HNE00702.1"
                     /translation="MNDPKKTLVQGLRQAVPYINALEHKTLVILFGGEAVVDVERFAS
                     LIHDIALLDSLGLKLVLVHGARPQVEAELAEMGHEPRFADGVRITDTVALDAVKEAVG
                     ATRILIEGLLSMGLANSPMHGARLRVGSGNAVVARPLGVREGVDFQYTGEVRRVDGEL
                     IRAWHQADTIVLLSPLGYSPTGEVFNVSAREVAVMAARTLGAEKLVMLTEEGGIVDHD
                     GRLLSDLSVPQAERLLAADTAMLDADQSEALDNAIAACRGGVRRVHLVPRKLDGALLL
                     ELFTRDGVGTMVFADQYEGARPATIDDVGGLIELIRPLEEDGTLVRRSRERLENEIDR
                     FTLLERDGAIIGCAALYAFPDEAIGELACVAVHADYRRGGRADALLGAIEARARGMGL
                     TRLFVLTTRTAHWFRERGFEPADLDALPVGKREMYNFQRRSKVFVKPLL"
     gene            complement(3807..4976)
                     /gene="argE"
                     /locus_tag="PLQ18_03275"
     CDS             complement(3807..4976)
                     /gene="argE"
                     /locus_tag="PLQ18_03275"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011628115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HNE00703.1"
                     /translation="MPVAAPDLLSMIRGLVALPSVSAVQPTWDQGNLAVVERLADWLE
                     ALGFRCELLALDDPRKANLVATLGSGPGGLVLAGHTDTVPYDSSGWRHDPFGGIEADG
                     RIYGLGTADMKSFLALAAEAARGLRAQDLRRPLVILATADEESSMDGARALVAAGRPR
                     GRHAVIGEPTGLKPIRLHKGVMMEAIRVQGRAGHASDPSLGNSALEGMHRVIGELLAM
                     RAGWATRYRNPQFAVEVPTLNLGHIHGGDNPNRICAHCELHIDIRPLPGMCLHALREE
                     IDAHLARALEGTGLGWSSEALIEGIEPFETPADSRLVAAAEQLTGHTAGSVAFGTEGP
                     YLNKLGMDTIILGPGDIAQAHQPDEYLALDRIPPTLELLRALIRRFCIDVPDPAA"
BASE COUNT          777 a         1678 c         1811 g          774 t
ORIGIN      
        1 agttcgctgg tctccagcgc gtggcgcagc ggcgagctgg agttgtcgag cagcacatcg
       61 gaaagcaccc gcgcgcgcgc ggtttcgagc ggatcggtgg tgcgcccgag cagccagccg
      121 agcacgacgt gcgtgccgtc ggcaagcgcc tcggcatcgg ccgcatacgg ggcttcgacc
      181 gcgaccggcg cgtcgtagcg gcgctcgtcc gcaatcgcca ggtcgagcgg ccgggccgtg
      241 aacgccgaca gcgcgcgttc ctcgaagtgc tgctgcagct cggcggccgg caggtcgccg
      301 tagctcatga acaccgcgtt cgacgggtgg taatgcgcgc cgtggaaggc gacgagtcgt
      361 tcatgcgtga gatcgggaat gaccccgggc tcgccgccgc tgttgtggtg ataggtcgtg
      421 gtcgggaaca gcgcgctctg caggcgctgc gccagcgcct gcaccgggct cgacatcgcg
      481 cccttcatct cgttgtagac gacgcccttg aacaccagcg gcgcgtcggc ggaatcatcg
      541 gcgaactcga cgcgatggcc ttcctgcgcg aagtcgagcg gatcgagcag cgggaagaag
      601 gcggcgtcga ggtagacatc gagcaggttc tcgaagtcct tgcggttgag gctcgcgaac
      661 ggatacgccg tccagtcggc gccggtgaag gcgttcatga aggtgttcag cgagcggcgc
      721 gtcatcatga agaacggatc gcgcaccggg tagcggcggc tgccgcagag cgccgtgtgt
      781 tcgaggatgt gcgcgacgcc ggtcgagtcc tgcggcacgg tcaggaacgc gaccagaaag
      841 gccttgtgcg gatcgtcgct cgccagatgc acgtgccggg cgccggtggc gcggtggcgg
      901 tactcctgca gttccagctt cagcgccggc acggcacggc tgcgcaggtg ttcgaaggcg
      961 gggtgcgtca tcgggcgtct cgggaaggca gtcggagcga tacgggccgg ccccctcggg
     1021 ccggatggaa aaagcgtatc agctttcgac cggaagcccc gctgacggtg ccgcaggatg
     1081 cagcctgaac gtcggcccgg aggtgggtgg taacctgttc agatcattac ttataccatc
     1141 atgtattcag caatcccgga acacgacagg catcgttccg tggcatggca gcaatgcatg
     1201 acgcgcagca gcagcaggcc gtgcgcagcg cagcggatgc acggctgcac cggcgcaggc
     1261 tccgggcagc ggggcagcgc gccgttcctg tcacgcccgc cgggcactgg cgctgcgacc
     1321 ggccgcgtgc ggcgcagccg cccggccgtg tcctgcggat cagtcgtgct tcagcggttc
     1381 gcgtcgcagg tcggtgaact gcgcgccgtc gtaggcgttc cagccgagca gcacgacgcc
     1441 gttcggcagg ccgagctgcg cggcctccca gcgcctagcg tgcagggact ggatgcggcg
     1501 gtagcacttc gggcaggaac agatctggcc gccgcaggta cacgtggcct gcatcgcggt
     1561 ttcccagtcg acgaattccc acacgcgcag acaatcctga caaagggcag ggaccatgta
     1621 gcactgtcct ccgggctgtt gatcccgatc gatcaaggtc ttgacggaag ctgtcgcgca
     1681 cacgggccgt gtcggctttg tcgcatgcag gcggcatcgc cggagaatgc gcaagaatcg
     1741 ggcctgttcc ggagggcgtg cggctgcggt gcccgccaat gaaaacggcc gcccgggggc
     1801 ggccgtttta gtttctgcgc gggcgcggag cttacagcgc caggatccag gcaatgattt
     1861 ccttcatgtc ggcgtccgac acctgcggat tcggcggcat cgggatctgg ccgaaggaac
     1921 cggcaccgcc ggccttgacc ttggcggcca gcttgtcggg ggccgtggcg tcacccttgt
     1981 acttcgcggc gacttccttg taggacgggc cgaccagctt ctggtcgacg gcgtggcagg
     2041 cgaggcagtt gttcttctgc gccagcgcga ggccgtcggc ctgcgccgga gcggcgacga
     2101 tcgcgccgag cagcagggcg cccgcgatgc cgtgaatggc tttcatgtgg ctctctcctc
     2161 gaagggggta caggggttca gtaccggcgg tccatgccgg gctgtgccgg cgggcgacag
     2221 ccgggcgggc gtccggggcg gggtggcaca ccggggccgc catcgccggc gcgcagtttt
     2281 gaccagaaat ccggctccgt cgagccgccg cagtcgctat tgcgtggtat gccgctgact
     2341 gccggccgga ttgccgtcat ccgaaaccgc gcgcccggat gttgccggtt gctgtgggct
     2401 gcggcgttgc gattgctcaa tgacacgcga agacagacga aaaaacctcg caccggctca
     2461 cagcagcggc ttcacgaaca ccttcgagcg gcgctggaag ttgtacatct cgcgcttccc
     2521 gaccggcagc gcatcgagat cggccggttc gaagccgcgt tcgcgaaacc agtgcgcggt
     2581 gcgcgtcgtc agcacgaaca ggcgcgtgag acccatgccg cgcgcgcgcg cctcgatcgc
     2641 cccgagcagc gcatcggcgc ggccgccgcg gcggtaatcg gcgtgcacgg ccacgcaggc
     2701 gagttcgccg atcgcctcgt ccgggaaggc gtacagcgcc gcgcagccga tgatcgcacc
     2761 gtcgcgttcg agcagcgtga agcggtcgat ctcgttctcc agccgctcgc gcgagcggcg
     2821 caccagcgtg ccgtcctctt cgagcgggcg gatcagctcg atcaggccgc cgacatcgtc
     2881 gatcgttgcc gggcgcgcgc cctcgtactg gtcggcgaac accatggtgc cgacgccatc
     2941 gcgcgtgaac agctccagca gcagcgcgcc gtcgagcttg cgcggcacca gatgcacgcg
     3001 gcgcacgccg ccgcggcagg cggcgatggc gttgtcgagc gcctccgact gatcggcgtc
     3061 gagcatcgcc gtatcggccg cgagcaggcg ctcggcctgc ggcacgctca ggtccgacag
     3121 cagccggccg tcgtgatcga cgatgccgcc ttcctcggtc agcatcacca gcttctcggc
     3181 cccgagcgtg cgcgccgcca tcaccgcgac ctcgcgcgcc gagacattga acacctcgcc
     3241 ggtcggcgaa tagccgagcg gcgacagcag cacgatggtg tcggcctgat gccaggcgcg
     3301 gatcagttcg ccgtcgacgc ggcgcacctc gccggtgtac tggaaatcga caccctcgcg
     3361 cacgccgagc ggccgggcga cgacggcgtt gccggagccg acgcgcagcc gcgcgccgtg
     3421 catcggcgag ttcgcaagcc ccatcgacag caggccctca atcaggatgc gcgtcgcgcc
     3481 gaccgcctcc ttgaccgcat cgagcgcaac cgtgtcggtg atgcgcacgc catcggcgaa
     3541 gcgcggttcg tggcccatct cggcgagttc ggcctcgacc tgcggccggg cgccgtgcac
     3601 gagcacgagc ttcaggccga gcgagtcgag cagcgcgatg tcgtggatca gcgaggcgaa
     3661 gcgctcgaca tcgacgacgg cctcgccgcc gaacaggatc accagcgtct tgtgttcgag
     3721 ggcattgatg tacggcacgg cctggcgcag gccctgcacc agtgttttct tgggatcgtt
     3781 catcgcgcgt tcggtcaggg agggcgtcat gcagcgggat cgggcacgtc gatgcagaag
     3841 cgacggatca gcgcgcgcaa cagttccagc gtcggcggaa tgcgatcgag cgccagatac
     3901 tcgtcgggct ggtgggcctg ggcgatgtcg ccggggccga ggatgatcgt gtccatgccg
     3961 agcttgttca ggtacggacc ttcggtgccg aaggccaccg aaccggcggt gtggccggtg
     4021 agctgctcgg ccgccgccac cagccgcgaa tcggccggtg tctcgaacgg ttcgatgccc
     4081 tcgatcagcg cctcgctgct ccagccgagg ccggtgcctt cgagcgcacg cgcaagatgc
     4141 gcgtcgatct cctcgcgcag cgcgtgcaga cacatgcccg gcagcggccg gatgtcgatg
     4201 tgcagctcgc agtgcgcgca gatgcggttc ggattgtcgc cgccgtggat gtggccgagg
     4261 ttcagcgtcg gcacctcgac cgcgaactgc ggattgcggt agcgcgtcgc ccagccggcg
     4321 cgcatcgcca gcagctcgcc gatcacgcgg tgcatgcctt cgagcgcgct gttgccgagg
     4381 ctcgggtccg aggcatgacc ggcgcggccc tgcacgcgga tcgcctccat catcacgccc
     4441 ttgtgcaggc ggatcggctt cagcccggtc ggttcgccga tcaccgcgtg gcggccgcgc
     4501 ggccggccgg cggcgaccag cgcacgcgcc ccgtccatgc tcgattcctc gtcggccgtc
     4561 gcgaggatca ccagcggccg gcgcaggtcc tgcgcacgca acccgcgcgc cgcctcggcc
     4621 gcgagcgcga ggaaggattt catgtcggca gtgccgagtc cgtagatgcg cccgtcggcc
     4681 tcgatgccgc cgaacgggtc gtggcgccag cccgaggaat cgtagggcac ggtgtcggtg
     4741 tgtccggcga gcaccagccc gcccgggccg ctgccgagcg tggcgacgag attggccttg
     4801 cgcggatcgt cgagcgcgag cagctcgcag cggaagccga gcgcttcgag ccagtcggcc
     4861 agccgttcga cgacggcgag attgccctga tcccaggtcg gctgcacggc actgaccgaa
     4921 ggcagcgcca cgagcccgcg gatcatgctg aggagatcgg gagcggcaac aggcatgttc
     4981 gggactcccg gggtgacggc agggacgggg attctagacc ggcggatcgc gcatccgttg
//
LOCUS       DAOQJH010000091         5005 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_9863_length_5005_cov_3.650101, whole genome shotgun sequence.
ACCESSION   DAOQJH010000091 DAOQJH010000000
VERSION     DAOQJH010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 5005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5005
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9863_length_5005_cov_3.650101"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..639)
                     /locus_tag="PLQ18_03280"
     CDS             complement(<1..639)
                     /locus_tag="PLQ18_03280"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNE00704.1"
                     /translation="MSTTPARALPDRSVADAAAAAHDLSALHLYAVLVEAGSFTRAAD
                     RLGCTKGHVSKQLAALERSLGMQLLHRSTRRLAPTAAGSALLPLARRLTAVDDEVRQV
                     LDGLRDDIAGTVRVSVPVSLGETCFDDIVVGFCRQYPRVHIALDLSNGIRDLVADGID
                     FALRSGSEPAPDLIARPLYSMEEYACASPAYLAAAGTPLAPADLAAHACLQNL"
     gene            752..1861
                     /locus_tag="PLQ18_03285"
     CDS             752..1861
                     /locus_tag="PLQ18_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkene reductase"
                     /protein_id="HNE00705.1"
                     /translation="MKTPANSAAALLSPYSLGPLTLRNRVVMAPLTRSRADAQTLAPR
                     ALNAEYYAQRAGAGLIISEGSQVSQQGQGYAWTPGIYSEAQIAGWRQVTDAVHALGGT
                     IFIQLWHVGRISHPELQPDGALPVAPSAIRPEGQAFTNDGFKPMVEPRALTTDEIPGI
                     VEQFRQAARNARAAGFDGAEIHSANGYLLEQFLRSHTNRRSDAWGGSLENRTRLLLEV
                     VAAVGSVFGLERTGVRLSPATTVNDIGPGEPELAAYVLKALDASGVGFVHMVEGVTGG
                     ARTVEGFDFRALRKLFPRTWIANNGYDGALGAEAVASGHAELVAFGKPWISNPDLVER
                     LATGLPLAQPDKATLYGGDAHGYTDYPRATPAAIA"
     gene            2083..2292
                     /locus_tag="PLQ18_03290"
     CDS             2083..2292
                     /locus_tag="PLQ18_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011813355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HNE00706.1"
                     /translation="MSEHVYKKIELVGSSPVSIEAAIENAVARAAQTLHSMRWLEVVE
                     TRGHIENGKVAHWQVTIRIGFTLDG"
     gene            2504..3136
                     /gene="ribA"
                     /locus_tag="PLQ18_03295"
     CDS             2504..3136
                     /gene="ribA"
                     /locus_tag="PLQ18_03295"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007071405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009231 - riboflavin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HNE00707.1"
                     /translation="MPAQPHPPLPPALVRRKVTVPLADGTPALFHSFDGLVDGREHLA
                     LGLGPQPADGIPLVRLHSECLTGDVFGSQRCDCGPQLEEALQRIGTAGGYVLYLRQEG
                     RGIGLYAKLDAYALQDRGLDTYAANRHLHQPEDARDYRCAAQMLVALGVTRIRLLSNN
                     PDKAEQLAQHGIEVIERVPTGVFLTAHNHAYLSAKVRFGSHDLDIDDTAP"
     gene            complement(3203..4468)
                     /locus_tag="PLQ18_03300"
     CDS             complement(3203..4468)
                     /locus_tag="PLQ18_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017878738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNE00708.1"
                     /translation="MSAPALAASRLPAMHHAGFTDPAGPQAGIRVGTPEYRQMRRALF
                     LGGFATFSLLYCVQPLLPLYSHEWQVAPAQASTVLSAATGGLALTLILASAVSDRLGR
                     RGLMAGAVLLASLLGLACSMAAGLAQLVWLRALEGIVLAGLPAVAMAYVSEEMEPRGL
                     ATAMGLYIAGSAFGGMSGRIATAWLAELWDWRSAMAAQALLSLGIGLVFWKSLPASRA
                     FRPRPLRLDALRAGLRLHLSDAGLPWLYALAFLLMGCFVSLYNYLGYRLAGAPFGFGA
                     GWIGTLSVFYVSGMLGSGWTGRLTARYGVQRVIMVMTLLMLAGLLATLSEALPLLLAG
                     VALATFGYFGAHAGASAWVGRRAREARGLASALYLSAYYAGSSLLGSVSGLVWHLDGW
                     SAVVALLATLLAGCLLIAWRLRRLAASEA"
     gene            4609..>5005
                     /locus_tag="PLQ18_03305"
     CDS             4609..>5005
                     /locus_tag="PLQ18_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008954324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNE00709.1"
                     /translation="MDLRRLRYFVAVAEERHFTRAAERLHIAQPPLSQQIQALEAELG
                     VALFERSRRHVTLTAAGERLLERARALLREAGAAAEEVRRIGRGELGELRIAFTSSLP
                     FSELIPRLLYDYRRRYPDVRLELRDTGTQR"
BASE COUNT          787 a         1844 c         1740 g          634 t
ORIGIN      
        1 caggttctgc aggcaggcgt gcgcggcgag atcggccggt gccagcggcg tgccggccgc
       61 ggccagatac gccggactcg cgcaggcgta ttcctccatg ctgtacagcg ggcgcgcgat
      121 caggtccggg gccggctcgc tgcccgagcg cagcgcgaag tcgatgccgt cggcgaccag
      181 atcgcggatg ccgttcgaca ggtcgagcgc gatgtgcacg cgcgggtact gccggcagaa
      241 gccgacgacg atgtcgtcga agcaggtctc gccgagcgac accggcaccg acacgcgcac
      301 ggtgccggcg atgtcatcgc gcaggccgtc gagcacctga cgcacctcgt catcgaccgc
      361 ggtcaggcgc cgcgcgagcg gcagcagcgc gctgccggcg gcggtcggcg ccagccggcg
      421 cgtcgagcgg tgcagcagct gcatgccgag gctgcgctcc agcgccgcca gctgcttgct
      481 gacgtggccc ttggtgcagc cgagccggtc ggcggcgcgc gtgaagctgc cggcctcgac
      541 cagcaccgca tacagatgca gcgccgacag atcgtgcgcg gcggccgcgg catcggcgac
      601 cgagcgatcc ggcagcgcac gggcaggggt cgtactcaaa ggttcgtttc ctctggtaaa
      661 cgatgcgttg ccggaatccc ggcttatcgg cggacaaagg ctttgtatag tgcgaatcgt
      721 ttcctgttcg caaccccatg aggccttgat catgaagacg cccgcgaatt ccgccgccgc
      781 cctgctgagc ccgtattccc tcggcccgct gaccctgcgc aaccgcgtgg tcatggcccc
      841 gctgacgcgc tcgcgtgccg atgcgcagac gctggcgccg cgcgcgctga acgccgagta
      901 ctacgcccag cgcgccggtg ccggcctgat catcagcgag ggctcgcagg tctcgcagca
      961 gggccagggc tatgcctgga cccccggcat ctacagcgag gcgcagattg ccggctggcg
     1021 gcaggtcacc gacgccgtgc acgcgctcgg cggcacgatc ttcatccagc tctggcacgt
     1081 cgggcgcatc tcgcacccgg agctgcagcc ggacggcgcg ctgccggtgg caccgtcggc
     1141 gatccgcccc gaaggccagg cgttcacgaa cgatggcttc aagccgatgg tcgagccgcg
     1201 cgcgctgacg acggacgaaa tccccggcat cgtcgaacag ttccggcagg cggcacggaa
     1261 cgcccgggcc gccggcttcg acggcgccga gatccacagc gccaacggct acctgctgga
     1321 gcagttcctg cgcagccaca ccaaccggcg cagcgacgcc tggggcggca gcctggagaa
     1381 ccgcacgcgc ctgctgctcg aagtcgtcgc cgcggtcggc agcgtgttcg ggctggagcg
     1441 caccggcgtg cggctgtcgc cggcgaccac ggtcaacgac atcggccccg gcgagccgga
     1501 actcgccgcg tacgtgctga aggcgctcga tgccagcggg gtcggcttcg tgcacatggt
     1561 cgagggcgtc accggcggtg cgcgcacggt cgagggcttc gatttccggg cgctgcgcaa
     1621 gctgttcccg cgcacctgga tcgccaacaa cggctacgac ggtgccctcg gtgccgaagc
     1681 cgtggccagc ggtcatgccg agctggtcgc cttcggcaag ccgtggatct ccaaccccga
     1741 cctggtcgaa cgcctcgcga ccggcctgcc gctcgcgcag ccggacaagg cgaccctcta
     1801 cggcggcgat gcgcacggct ataccgacta tccgcgcgcc acgccggcgg cgattgcctg
     1861 aacgcgggca tgcgcgggcg ctcgtcgtgc gcgacgccgc ccaccgccgc cgggcccgcg
     1921 ctgccgcgtg cttccggcga ccagtcgctg cggattgccg cgctgcggga ttgctttctg
     1981 tgcgtccggg gtctggagtt cggagcgcgc cgcggcatgg acccgcccgc ggcgtgcgac
     2041 gtttccgggc gcgggtccgc accgaaggag caggcacatc ccatgagcga gcacgtctac
     2101 aagaagatcg aactcgtcgg ctcgtcgccg gtatcgatcg aggcggccat cgagaacgcc
     2161 gttgcccgcg ccgcgcagac cctgcacagc atgcgctggc tggaagtcgt cgagacgcgc
     2221 ggccacatcg agaacggcaa ggtcgcgcac tggcaggtga cgatccgcat cggtttcacg
     2281 ctcgacggct gagcctgcga gccacagagc gggacgggct ggatgccggg cgcacaagct
     2341 gcgaaagtgg cgggcttcgg tccgcccgct gcggcggaca ccggccgcac cggcaccggc
     2401 cgggttccgg acgccggcgg cgcacggcgc gcatcgaggt ctgccgaccc gcgtgcgcca
     2461 gcgctgccgg cgtgcagccc gacccaccac ggacacctgc aacatgccag cccagcctca
     2521 cccgccgctt ccgcccgcgc tcgtgcgccg caaggtcacg gtaccgctcg cggacggcac
     2581 gcccgcgctg ttccacagct tcgacggcct cgtcgacggg cgcgagcacc tcgcgctcgg
     2641 cctcggcccg cagccggccg acggcatccc gctggtgcgc ctgcattccg aatgcctgac
     2701 cggcgacgtg ttcggctcgc agcgctgtga ctgcggtccg cagcttgagg aagccctgca
     2761 gcgcatcggc actgccggcg gctacgtgct gtacctgcgc caggagggcc gcggcatcgg
     2821 cctgtacgcc aagctcgatg cctacgcgct gcaggatcgc ggcctcgaca cctacgccgc
     2881 caaccgccat ctgcaccagc ccgaggacgc gcgcgattac cgctgcgccg cgcagatgct
     2941 cgtcgccctc ggcgtcacgc gcatccgcct gctcagcaac aacccggaca aggccgaaca
     3001 gctcgcgcag cacggcatcg aggtcatcga gcgcgtgccg accggcgtgt tcctgaccgc
     3061 acacaaccat gcgtacctca gcgccaaggt gcgcttcggc agccacgacc tcgacatcga
     3121 cgacacggcg ccgtgatccg gcgcccgctg cggcgacagg cgcggcttct ttgaagaggc
     3181 ccgccgggcc ggggcgaggc gttcaggctt cgctcgccgc cagccggcgc agccgccagg
     3241 cgatcagcag gcagccggcg agcagcgtcg cgagcagcgc gacgacggcg ctccagccat
     3301 cgaggtgcca gaccagcccc gagacgctgc cgagcaggct ggaaccggcg tagtacgcgc
     3361 tgagatacag cgccgaagcc agtccgcgtg cctcgcgcgc ccgccgcccg acccaggcgc
     3421 tcgcgccggc gtgggcaccg aagtagccga aggtcgcgag ggcgacgccg gcgagcagca
     3481 gcggcagcgc ctccgacagc gtcgcgagca gcccggcgag catcagcagc gtcatcacca
     3541 tgatcacgcg ctgcacgccg tagcgcgccg tgagccgccc ggtccagccc gagccgagca
     3601 ttccggacac gtagaacacc gacagcgtgc cgatccagcc ggcaccgaag ccgaacgggg
     3661 cgccggccag ccggtagccg aggtagttgt acaggctgac gaaacagccc atcagcagga
     3721 aggcgagcgc gtacagccac ggcaggccgg catcggacag atgcaggcgc agcccggcgc
     3781 gcagcgcatc gaggcgcagc ggccgcggcc ggaacgcccg cgaagccggc aggctcttcc
     3841 agaacaccag cccgatcccc agcgacagca gggcctgcgc ggccatcgcg ctgcgccagt
     3901 cccacagctc cgcgagccag gccgtggcga tgcgcccgct catgccgccg aaggcgctgc
     3961 cggcgatgta gaggcccatc gccgtcgcca gcccgcgcgg ctccatttcc tcggacacat
     4021 aggccatcgc caccgccggc aggccggcga gcacgatgcc ttcgagcgca cgcagccaca
     4081 cgagctgcgc cagtcccgcg gccatcgagc aggccagtcc gagcagcgac gcgagcagca
     4141 cggcgccggc catcagcccg cgccggccga ggcgatcgga cacggcgctc gcaaggatca
     4201 gcgtcagggc cagcccgccg gttgccgccg acagcaccgt gctcgcctgc gccggtgcca
     4261 cctgccattc gtgcgagtac agcggcagca gcggctgcac gcagtacagc agcgagaagg
     4321 tcgcgaagcc gccgaggaac agcgcccggc gcatctgccg gtattcgggc gtaccgacgc
     4381 ggatgccggc ctgcggccct gcaggatcgg tgaaaccggc atggtgcatg gcgggcaggc
     4441 gggaagcagc aagggcaggg gcggacatcg gacatcaccg agcggggatc acggatgcac
     4501 aaggctaggg cgctgcagtc tgataggtcc aatatggttt tcaggcagta ttgatatcgt
     4561 ttcgatatca gtcctgcccc cgtaccgtcc ttgtccggag ccgccgtcat ggacctgcgc
     4621 cgcctgcgct atttcgtcgc cgtcgcggag gagcgccact tcacgcgcgc cgccgaacgc
     4681 ctgcacatcg cgcagccgcc gctgagccag cagattcagg cgctcgaagc cgaactcggc
     4741 gtcgcgctgt tcgagcgctc gcgccggcac gtgacgctca ccgccgccgg cgagcgtctg
     4801 ctggagcggg cgcgcgcgct gctgcgcgag gccggggcgg ccgccgagga ggtccggcgc
     4861 atcggccgcg gcgaactcgg cgagctgcgc atcgccttca cgagctcgct gccgttctcg
     4921 gaactgattc cgcgcctgct gtatgactac cgccgccgct atccggacgt gcggctggaa
     4981 ctgcgcgaca ccggcacgca gcgcc
//
LOCUS       DAOQJH010000092         4973 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_9968_length_4973_cov_4.068727, whole genome shotgun sequence.
ACCESSION   DAOQJH010000092 DAOQJH010000000
VERSION     DAOQJH010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 4973)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4973)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4973
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9968_length_4973_cov_4.068727"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="PLQ18_03310"
     CDS             <1..255
                     /locus_tag="PLQ18_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008059325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNE00710.1"
                     /translation="LIGTAPDKASVASFVLAGYRTDEVGTALNREGIAVRSGHHCAQP
                     ILRRFGLETSVRPSLAFYNTREEIDRMIRVLQQLARERRV"
     gene            complement(499..1218)
                     /locus_tag="PLQ18_03315"
     CDS             complement(499..1218)
                     /locus_tag="PLQ18_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008738495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HNE00711.1"
                     /translation="MPDDLLRALFADPDLPELLIRLGCAWGVGALIGLERTFRGRPAG
                     FRTHALVCLASALLMLLGVQPQAWLEDADAALRTDPTRVAQGIMTGVGFIGAGVIFRE
                     GLTVRGLTTAASLWITAVLGLLYGAGYFEPAVIVSLLTLATLSAFHWIEKRVPAWHYA
                     QLTVRYDTAAVPDEATLRSRFAACRVGITQIGYRLTENGRRFEYRLVVRTRSSAGFEP
                     LCRQLRAETAVPAFRLAPMGD"
     gene            complement(1485..2240)
                     /locus_tag="PLQ18_03320"
     CDS             complement(1485..2240)
                     /locus_tag="PLQ18_03320"
                     /inference="COORDINATES: protein motif:HMM:NF016154.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HNE00712.1"
                     /translation="MEAPDWAKLWLGNESPDFLIEIALRSVVMYVALMLWLRLLGRRT
                     LGQLSILEFGMVIILGSAAGDPTFYDDVPLLHGIAVIAVITLLQLGYTQLLARSEKVE
                     TLMEGKPVELVRDGVLQVRAMAGVPMNPEEAFEALRLRDIEQLGQVRRAYIEQGGQIS
                     VLRWRPEETPPGLPLIPPWDLEQPPKWHADALPQAGGLRFCCANCGLCPDTTPDGPCP
                     HCDCDEWTVAVQRALEPLPVALTRIATPIPEHA"
     gene            complement(2313..2801)
                     /gene="folA"
                     /locus_tag="PLQ18_03325"
     CDS             complement(2313..2801)
                     /gene="folA"
                     /locus_tag="PLQ18_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012639297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HNE00713.1"
                     /translation="MLSQIVAIGRNRVIGAGNALPWRLPDDLAHFKRLTLGKPVLMGR
                     KTWESLGRPLPGRDNLVITRNPGYHAAGARVFASLDTALAACSDAPEIMLIGGAELYA
                     QTLPICDRLYLTEIDAAPDGDAFFPALDPADWRETAAEPHPADARHAHAFTWRTLERV
                     RR"
     gene            complement(2812..3093)
                     /locus_tag="PLQ18_03330"
     CDS             complement(2812..3093)
                     /locus_tag="PLQ18_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018837650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HNE00714.1"
                     /translation="MVTALVLLNTEKGRINEVAETLAGIPGITEVYSLAGVHDLAAII
                     RVDSNDALADVVTHRMLKVEGIRDSQTHVAFRVHSRHDLDGMFAIGGEL"
     gene            complement(3204..4052)
                     /locus_tag="PLQ18_03335"
     CDS             complement(3204..4052)
                     /locus_tag="PLQ18_03335"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018233662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HNE00715.1"
                     /translation="MAVYAIGDVQGCYEPLLRLLDKLHFDPDTDRIWLTGDLVNRGPH
                     SVETLRHISALGPAATTVLGNHDLTLLAVAEGHVKPGKKDTFYDVLDAPDAAELLAWV
                     RGLPMLHHDDALGFTMVHGGLSPHWDLALAQACAHEVEAVLRGPGYPEFLAHMFGSEP
                     RAWRDDLEGLPRLRHIVNVMTRMRFVFADGSLCYDQKGAPGSQPPDLMPWFEVPWRRN
                     ADLRIITGHWAALGYFRARGIYALDSGCIWGQHLTAFRLDAHEVPCCVDCWEMRKVKN
                     REDQGI"
     gene            complement(4209..>4973)
                     /locus_tag="PLQ18_03340"
     CDS             complement(4209..>4973)
                     /locus_tag="PLQ18_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012970748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III restriction endonuclease subunit R"
                     /protein_id="HNE00716.1"
                     /translation="FRLQRLLFVAARKAFEQLRPGYEGSREWLVQQLIRLVERFFASD
                     RLEIPSRFHQDPLRRRILIALSIDEIVQHVIGQIEQRNVERLEPVFDPQWPIGSTRQM
                     RTWYTTRPCLETAKSQISHAVADSTWEQYTVGRLEACAGVAAFARNDHLGFQVHYLWH
                     GSRRRYVPDFLIRFANGKQMVLEIKGEDSEQDRCKRRALDAWVQAVNSRGGFGVWCRD
                     VVCGEPARIDDVLDHHARTGGASMPVGAAQFSLGTG"
BASE COUNT          784 a         1681 c         1779 g          729 t
ORIGIN      
        1 ctgatcggca cggcgcccga caaggcgagc gtggcgtcct tcgtgctcgc cggctaccgc
       61 accgacgagg tcggcacggc gctgaaccgc gaaggcatcg ccgtgcgctc cgggcatcac
      121 tgcgcgcagc cgatcctgcg ccgcttcggt ctggagacct cggtgcgccc gtcgctggcc
      181 ttctacaaca cgcgcgagga aatcgaccgg atgatccgcg tgctgcagca actggcgcgc
      241 gagcgccggg tctgacgccg ctcactgcga ttcgcgctgc cggttccgcc cggccctccc
      301 gtccttatgg gcgagggccg gaaggaacta aggaagaagc gcgcgggcgc gcgtgagggt
      361 tccggacagc ctccgccggc ccgaccgtca ctgcgcggcc gggccggttc cggttttcag
      421 gccgggatca gcgccagata cagcacggcc tcgccctgac cgggattgcg ctatgcgggc
      481 gccagcgaag ggcgggcgtc agtcgcccat cggtgccagc cggaaggccg gcacggcggt
      541 ctcggcgcgc agttgccggc acaggggttc gaagccggcg gaactgcgcg tgcgcacgac
      601 cagccggtat tcgaagcgcc ggccgttttc ggtgagccgg tagccgatct gcgtgatgcc
      661 gacgcggcag gcggcgaagc ggctgcgcag cgtggcctca tcgggtacgg ccgccgtgtc
      721 gtagcgcacg gtcagttgcg cgtagtgcca cgccggcacg cgcttctcga tccagtggaa
      781 tgccgacagg gtcgccagcg tcagcagcga cacgatcacg gccggttcga agtagccggc
      841 cccgtacagc aggccgagca cggcggtgat ccacagcgac gcggccgtgg tcagcccgcg
      901 taccgtcagc ccctcgcgga agatcacgcc ggcgccgatg aagccgacgc cggtcatgat
      961 gccctgcgcg acgcgcgtcg gatcggtgcg cagcgcggca tcggcatcct caagccaggc
     1021 ctgcggctgc acgccgagca gcatcagcag cgccgaagcc agacagacca gcgcgtgcgt
     1081 gcggaagccg gccggacggc cgcggaaggt gcgttcgaga ccgatcagcg caccgacccc
     1141 ccaggcacag ccgagccgga tcagcagctc gggcaggtcg ggatcggcga acagggcgcg
     1201 gaggagatcg tcgggcatcg gggtgtgccg tgaacgggca acgccagccg gcagcgcggg
     1261 aacgacagaa aaggcggcac cggttcgtcc ggatgaaagc tggacgccgc agggccagtg
     1321 ggacggccga ggcgaacgaa ccggtacctg gagtccccgc cggtcgttcg gcccaagctg
     1381 acggaagccc ggtcgtccgg tgcggagacc ttgcaggtat agcggcagcg gcggcggcag
     1441 ggcgttaacc ttatttcata tttgaacgcc ccggcgcgcc cgcctcaggc gtgttcgggg
     1501 atcggtgtgg cgatgcgggt cagggccacc ggcagcggct ccagcgcccg ctgcacggca
     1561 accgtccatt catcgcagtc gcagtgcggg caggggccgt ccggcgtggt gtccgggcac
     1621 aggccgcagt tcgcgcagca gaagcgcagg ccgccggcct gcggcagcgc atcggcgtgc
     1681 catttcggcg gctgttcgag atcccagggc gggatcagcg gcaggccggg cggggtttcc
     1741 tcgggccgcc agcgcagcac gctgatctgt ccgccctgct cgatgtaggc gcggcgcacc
     1801 tgaccgagct gttcgatgtc gcgcaggcgc agcgcctcga aggcttcctc ggggttcatc
     1861 ggcacgccgg ccatcgcgcg cacctgcagc acgccgtcgc gcacgagttc gaccggcttg
     1921 ccttccatca gcgtctcgac cttctcggag cgcgccagca gctgcgtgta gccgagctgc
     1981 agcagcgtga tcaccgcgat cacggcgatt ccgtgcagga ggggcacgtc gtcgtagaag
     2041 gtcggatcgc cggccgccga gccgaggatg atgaccatgc cgaattcgag gatcgacaac
     2101 tgcccgagcg tgcgccgccc gagcagacgc agccacagca tcagcgccac gtacatgacg
     2161 acgctgcgca gcgcgatctc gatcaggaaa tccggtgact cgttgccgag ccagagcttg
     2221 gcccagtccg gcgcttccat cgtcgcatcc ccccggtggt cgttctcgcg ctccgaccgg
     2281 gatgcggggc cggcggttcc ggcggcaggt tttcagcggc gcacgcgctc cagcgtccgc
     2341 cacgtgaagg cgtgggcgtg gcgcgcatcg gccggatgcg gctcggcagc ggtctcccgc
     2401 cagtcggccg gatcgagcgc cgggaagaac gcatcgccgt ccggcgcggc gtcgatctcg
     2461 gtcaggtaga ggcggtcgca gatcggcagc gtctgcgcgt acagctcggc accgccgatc
     2521 agcatgatct cgggtgcatc gctgcaggcg gcgagtgcgg tatcgagcga cgcaaacaca
     2581 cgcgcaccgg cggcgtggta gccgggattg cgtgtgatga cgaggttgtc gcgccccggc
     2641 agcggccggc ccagcgactc ccaggtcttg cgccccatca gcaccggctt gccgagcgtc
     2701 aggcgcttga agtgcgcaag gtcatcgggc aggcgccacg gcagcgcgtt gccggcgccg
     2761 atgacgcggt tgcggccgat ggcgacgatc tggctcagca tgcgcgcagc atcacagctc
     2821 gccgccgatc gcgaacatgc cgtcgaggtc gtggcgcgag tgcacgcgga aggcgacgtg
     2881 cgtctgcgag tcacggatgc cctcgacctt cagcatccgg tgcgtgacca cgtcggcgag
     2941 cgcgtcattg ctgtcgacgc ggatgatcgc ggccagatcg tgcacgccgg ccagcgaata
     3001 gacctcggtg atgccgggga tgccggcgag cgtctcggcg acctcgttga tgcggccctt
     3061 ctcggtgttc agcaatacca gtgcggtgac catgtccgta tctctcgtgc cggtggggcg
     3121 ttcgtcgtca tcgtcgagcg ctgcgctgcc gccgcccgct ggcgccgcgt ggcggaagcc
     3181 gctcgcatgc ggtgggaccg ggctcagatg ccctgatcct cacggttctt gaccttgcgc
     3241 atctcccagc agtcgacgca gcacggcacc tcgtgggcat cgaggcggaa cgccgtcagg
     3301 tgctggcccc agatgcagcc ggagtcgagc gcgtagatgc cgcgggcccg gaagtaaccg
     3361 agcgccgccc agtggccggt gatgatgcgc agatcggcgt tgcgccgcca cggcacctcg
     3421 aaccacggca tcaggtccgg cggctggctg ccgggcgcgc ctttctggtc atagcacagc
     3481 gagccgtcgg caaacacgaa gcgcatgcgc gtcatgacgt tgacgatgtg ccgcagccgc
     3541 ggcagtcctt cgaggtcatc gcgccaggcg cgcggctcgg agccgaacat gtgcgcgagg
     3601 aactccggat agcccgggcc gcgcagcacg gcctcgacct cgtgcgcgca ggcctgcgcc
     3661 agcgccagat cccagtgcgg cgacagcccg ccgtggacca tcgtgaagcc gagcgcgtca
     3721 tcgtgatgca gcatcggcag cccgcgcacc caggcaagca gctcggcggc gtcgggcgcg
     3781 tcgagcacgt catagaaggt gtccttcttg cccggcttga cgtggccttc ggcgaccgcg
     3841 agcagcgtca ggtcgtgatt gccgagcacg gtggtcgcgg ccgggccgag ggcgctgatg
     3901 tggcgcagcg tctcgaccga atgcggaccg cggttgacca gatcgccggt cagccagatg
     3961 cggtcggtgt cggggtcgaa atgcagcttg tcgagcaggc gcagcagggg ctcgtagcag
     4021 ccctgcacgt cgccgattgc gtaaacggcc atgcggtctc cggaaggcac ggcacgctga
     4081 ctggcgcaac aagccggaca gcgcgggggg agggcgggat gttgtgccgc agcatgccgc
     4141 agaaatacgc cggctttcca gtgcggatgc aatggttttc aggatacgga atatggcagc
     4201 cggctgcctc atccggtccc gaggctgaac tgcgccgcac cgaccggcat cgacgccccg
     4261 cccgtacggg cgtgatggtc gagcacgtcg tcgatgcgcg ccggctcgcc gcagaccaca
     4321 tcccggcacc agacgccgaa accgccgcgg ctgttgaccg cctgcaccca ggcatcgagc
     4381 gcacggcgtt tgcagcggtc ctgctccgag tcctcgccct tgatctccag caccatctgc
     4441 ttgccgtttg cgaagcggat caggaagtcc ggcacgtagc gccggcggct gccgtgccag
     4501 aggtagtgca cctgaaagcc gagatggtca ttgcgcgcga aggcggcgac tccggcgcag
     4561 gcttccagcc gcccgaccgt gtactgctcc caggtgctgt cggcgaccgc gtggctgatc
     4621 tgcgacttcg cggtttcgag acacggccgg gtcgtgtacc aggtgcgcat ctgccgggtc
     4681 gagccgatcg gccattgcgg atcgaacacc ggctccagcc gctcgacgtt gcgctgttcg
     4741 atctgcccga tgacgtgctg aacgatctcg tcgatcgaca gcgcgatcag gatgcgccgg
     4801 cgcagcgggt cctgatggaa gcgcgacgga atctccagcc ggtccgacgc gaagaagcgc
     4861 tcgaccagcc ggatcagttg ctgcaccagc cactcgcggc tgccttcgta gcccggacgc
     4921 aactgctcga atgccttgcg cgcggcgacg aacagcaggc gttgcaggcg gaa
//
LOCUS       DAOQJH010000093         4968 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_9982_length_4968_cov_4.023407, whole genome shotgun sequence.
ACCESSION   DAOQJH010000093 DAOQJH010000000
VERSION     DAOQJH010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 4968)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4968)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4968
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9982_length_4968_cov_4.023407"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            250..2082
                     /gene="glmS"
                     /locus_tag="PLQ18_03345"
     CDS             250..2082
                     /gene="glmS"
                     /locus_tag="PLQ18_03345"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012639831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HNE00717.1"
                     /translation="MCGIVGAIAERNVVPILLEGLRRLEYRGYDSAGIATLDDERRLQ
                     RVRAVGKVQALTDSLAANPAPGRLGIAHTRWATHGVPSERNAHPHVSGSELALVHNGI
                     IENYKDLKAELEGLGYRFESETDTEVVAHLVARYLAEERDLLAAVQRAVSRLTGAYSL
                     GVISRHEPGRLIAARQGSPLVIGIGIGEHFIASDVFALLPVTQEFVFLEEGDVADLSR
                     SAFTIYARDGRLVERARATSAQSAGVADKGPYRHYMLKEIHEQPAVVADTLEGRLHDG
                     RVLESCFGHEAGALFDQVRQVHIVACGTSYHAGLVARYWLEAWGVPATVEVASEYRYR
                     DPVIPPGTLFVSISQSGETADTLAALRGAKARGCLASLAICNVPESSLVRESALVLMT
                     RAGREIGVASTKAFTTQLVALRLLAIVLGRRHSLDAAAETQAVAELMQLPARLEQALA
                     LDPVVEHLAETFGAKAHALLLGRGAQFPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALVDPAMPVICVLPDNALHEKVISNLQEVRARGGELFLFADEAVDVHLDGDAVHVLRI
                     APTPDSIAPMVFTVPLQLLAYHVAVLRGTDVDQPRNLAKSVTVE"
     gene            2138..3415
                     /gene="tviB"
                     /locus_tag="PLQ18_03350"
     CDS             2138..3415
                     /gene="tviB"
                     /locus_tag="PLQ18_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011396568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Vi polysaccharide biosynthesis
                     UDP-N-acetylglucosamine C-6 dehydrogenase TviB"
                     /protein_id="HNE00718.1"
                     /translation="MMDLSNVRIGLIGLGYVGLPLAVEFGKHYPTVGFDIKADRVAEL
                     QAGRDSTLEVEAEELALADQLSYTSALEDIRACNVYIVTVPTPIDAAKRPDLTPLVKA
                     SETLGKVLKRGDVVVYESTVYPGATEEVCVPILEAVSGLKFNLDFFAGYSPERINPGD
                     KEHRVTTIRKVTSGSTPEVAEFVDRLYGSIIVAGTHRASSIKVAEAAKVIENTQRDVN
                     IALINELALIFGRLGIDTEEVLLAAGTKWNFLPFRPGLVGGHCIGVDPYYLTHKAQEI
                     GYHPEVILAGRRINDGMGVHVAECVVKLMTQKRMHVVGSRVLVMGLTFKENCPDLRNT
                     RVTDIIAELKTYHAHVDVYDPWVSVAEAEHEYGIHPVSELIDGQYDAIILAVAHNEFR
                     KLGIERIRALGKPGAVLYDIKYVLPRSAVDGRL"
     gene            3495..4616
                     /gene="wecB"
                     /locus_tag="PLQ18_03355"
     CDS             3495..4616
                     /gene="wecB"
                     /locus_tag="PLQ18_03355"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HNE00719.1"
                     /translation="MSAKILCVVGARPNFMKIAPIMAAFRARAPQLVVKLVHTGQHYD
                     VAMNDRFFEQLGIPTPDISLEVGSASHAVQTAEIMKRFEPVLLEERPAAVLVVGDVNS
                     TIACALVAAKLGVRVVHVEAGLRSRDRTMPEEINRILTDQLSDRLYTTEAEALDNLRA
                     EGIDAARVCFAGNVMIDTLRASEAQAVPAAQTLAAAADPAVFRQAGHALLTLHRPSNV
                     DDPVVLARLLGVIDEVSRELPVVFPVHPRTQGKIREAGLGAVLDGTRWLVLPPVGYFE
                     MLGLMREARLVLTDSGGLQEETTALGVPCLTLRENTERPITIAQGTNTLVGTDADAIR
                     AAVREVLATGGKAGRTPERWDGHAAGRIVADLAEWLAPA"
     gene            4660..>4968
                     /locus_tag="PLQ18_03360"
     CDS             4660..>4968
                     /locus_tag="PLQ18_03360"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HNE00720.1"
                     /translation="MKILVTGAAGFIGFHLTQRLLARGDEVIGLDNLNDYYDVALKQA
                     RLAQLEAQPGFRFVGLDLADRPGIAALFEAERFARVVNLAAQAGVRYSLKNPHAYVDS
                     N"
BASE COUNT          798 a         1741 c         1654 g          775 t
ORIGIN      
        1 agccgcgtga cggtcgcggt cgtgccgacc gacgaggaag gcgtcatcgc ccggcacgca
       61 cgccggctga cgctggggtg aggaagcccg gggcgtgagc gggcggtgcc ctgctcacgc
      121 ctcatggccg gacttcccgt ttatccacgt gaacaatcat cgaccgtccc gccggccggc
      181 ttgtcggtcg gcggcgcggg tcggtacgct ggcgccccgt atcctcccgc tgcaacagga
      241 atccttgcca tgtgcggaat cgtcggcgcc atcgccgagc gcaacgtcgt cccgatcctg
      301 ctggagggac tgcgccggct cgaataccgc ggctacgact ccgccgggat tgcgaccctg
      361 gatgacgagc gtcgtctcca gcgcgtgcgc gcggtcggca aggtccaggc gctcaccgac
      421 agcctggcgg cgaatcccgc tccgggccgg ctcggcatcg cacacacgcg ctgggccacg
      481 cacggcgtgc cgagcgagcg caacgctcat ccgcacgtca gcggcagcga gctcgcgctg
      541 gtgcacaacg gcatcatcga gaactacaag gatctgaagg ccgaactcga aggactcggc
      601 tatcgcttcg aatccgagac cgacaccgag gtcgtcgcgc atctggtcgc gcgctacctg
      661 gccgaggagc gcgacctgct cgccgcggtg cagcgcgcgg tgtcgcgcct gaccggcgcc
      721 tacagcctcg gcgtgatctc gcgccacgag ccgggccggc tgatcgccgc acgtcagggc
      781 tcgccgctgg tgatcggcat cggcatcggc gagcacttca tcgcgtccga cgtgttcgcg
      841 ctgctgccgg tcacgcagga gttcgtgttc ctggaggaag gcgatgtcgc cgacctgtcg
      901 cgcagcgcgt tcacgatcta cgcgcgcgac ggccggctgg tcgagcgtgc acgcgcgacc
      961 tcggcgcaga gtgctggtgt cgccgacaag gggccgtacc ggcactacat gctcaaggaa
     1021 atccacgagc agccggccgt ggtcgccgac acgctcgaag gccggctgca cgacggccgc
     1081 gtgctggaaa gctgcttcgg tcacgaggcc ggcgcgctgt tcgatcaggt gcgccaggtc
     1141 cacatcgtcg cctgcggcac cagctatcac gccggtctgg tcgcgcgtta ctggctcgaa
     1201 gcctggggcg tgccggccac ggtcgaggtc gcgagcgaat accgctaccg cgacccggtg
     1261 atcccgcccg gcacgctgtt cgtcagcatc tcgcagtccg gcgagaccgc cgacacgctt
     1321 gcggcgctgc gcggtgcgaa ggcacgcggg tgtctggcga gcctcgcgat ctgcaacgtg
     1381 ccggaaagct cgctggtgcg cgaatcggcg ctggtgctga tgacccgcgc cggccgcgag
     1441 atcggtgtgg cctcgaccaa ggcgttcacg acccaactgg tggcgctgcg cctgcttgcg
     1501 atcgtgctcg gtcgccgcca cagcctcgac gcggcagccg agacacaggc cgtcgccgag
     1561 ctcatgcaac tgccggcacg cctggagcag gcgctggcgc tcgatccggt cgtcgagcat
     1621 ctggccgaga ccttcggggc caaggcgcac gcgctgttgc tcgggcgcgg cgcgcagttc
     1681 ccgatcgccc ttgaaggggc gttgaagctc aaagaaatca gctacatcca tgcagaggcc
     1741 tacgcggccg gcgaactcaa gcacggtccg ctggcgctgg tcgatccggc gatgccggtg
     1801 atctgcgtgc tgccggacaa cgcgctgcac gagaaggtga tctcgaacct gcaggaagtg
     1861 cgcgcccgtg gcggcgagct gttcctgttc gccgacgagg cggtcgatgt gcatctggat
     1921 ggcgacgccg tgcacgtgct gcgtatcgca ccgaccccgg attcgatcgc cccgatggtg
     1981 ttcacggtgc cgctgcagct gctggcctac cacgtcgcag tgctgcgcgg caccgatgtc
     2041 gaccagccgc gcaacctcgc gaaatcggtg acggtggagt agggcgccgc cgcccgccag
     2101 gccggaattc ctcccccttt cccctccaga gagaccaatg atggatctca gcaacgtccg
     2161 catcggcctg atcggcctcg gctacgtcgg cctgccgctg gccgtcgagt tcggcaagca
     2221 ctatccgacc gtcggtttcg acatcaaggc cgatcgcgtc gccgagctgc aggccggccg
     2281 cgacagcacg ctcgaagtcg aagccgagga actcgcgctt gccgatcaac tgagctacac
     2341 gagcgcgctc gaagacatcc gtgcctgcaa tgtctacatc gtcacggtgc cgacgccgat
     2401 cgacgccgcc aagcgtccgg atctcacgcc gctcgtgaag gccagcgaga cgctcggcaa
     2461 ggtgctcaag cgcggcgacg tggtcgttta tgaatcgacg gtgtatccgg gcgcgaccga
     2521 ggaggtctgc gtgccgatcc tcgaagcggt gtcgggcctg aagttcaacc tcgacttctt
     2581 cgccggctac agccccgagc gcatcaatcc cggcgacaag gaacaccgcg tcacgaccat
     2641 caggaaggtc acctccggct cgacgcccga ggtcgccgag ttcgtcgacc ggctgtacgg
     2701 cagcatcatc gtcgccggca cgcaccgcgc gagcagcatc aaggtcgccg aggccgccaa
     2761 ggtcatcgag aacacgcagc gcgacgtgaa catcgcgctg atcaatgagc tggcgctgat
     2821 cttcggtcgt ctcggcatcg acaccgagga agtcctgctc gccgccggca ccaagtggaa
     2881 cttcctgccg ttccggcccg ggctggtcgg cgggcactgc atcggcgtcg atccgtacta
     2941 cctgacgcac aaggcgcagg agatcggcta tcaccccgag gtcatcctcg ccggccggcg
     3001 catcaacgac ggcatgggcg tgcacgtggc tgaatgcgtg gtcaagctga tgacgcagaa
     3061 gcgcatgcac gtggtcggct cgcgcgtgct ggtcatgggc ctgaccttca aggaaaactg
     3121 cccggacctg cgcaacaccc gcgtcaccga catcatcgcc gaactgaaga cctatcacgc
     3181 gcacgtcgat gtctacgatc catgggtctc ggtggccgag gccgaacacg agtacggcat
     3241 ccatccggtc tcggagctga tcgacggcca gtacgacgcg atcatccttg ccgtcgcgca
     3301 caacgagttc cgcaagctcg gcatcgagcg catccgcgcc ctcggcaagc ccggcgccgt
     3361 gctctacgac atcaagtacg tgctgccgcg cagcgccgtc gacggccggc tctgagcttc
     3421 ggccttcgcc tgccaccgaa acgccgcccg cccccgaagc gggcggtttc gcatccgtca
     3481 gctccggtaa gtccatgtca gcgaagattc tctgcgtcgt cggcgcccgg ccgaacttca
     3541 tgaagatcgc cccgatcatg gccgcgttcc gtgcccgggc cccgcaactg gtcgtcaagc
     3601 tcgtgcacac cggccagcac tacgatgtgg cgatgaatga ccgcttcttc gagcagctcg
     3661 gcatcccgac gccggacatc tcgctcgagg tcggctcggc ctcgcacgcg gtgcagaccg
     3721 ccgaaatcat gaagcgcttc gagccggtac tgcttgagga acggccggcg gcggtgctgg
     3781 tggtcgggga tgtcaactcg acgatcgcct gcgcgctggt cgccgcgaag ctcggcgtca
     3841 gggtcgtgca cgtcgaggcc gggttgcgct cgcgcgaccg cacgatgccc gaggagatca
     3901 accgcatcct gaccgaccag ctctcggacc gcctgtacac gaccgaggcc gaggcgctgg
     3961 acaacctcag ggccgagggc atcgatgccg cgcgcgtctg cttcgccggc aacgtcatga
     4021 tcgacacgct gcgcgccagc gaggcgcaag ccgtaccggc cgcgcagacg ctggctgcgg
     4081 ccgcggatcc ggctgtgttc cggcaggctg gccatgcgct gctgacgctg caccgaccat
     4141 cgaacgtcga tgatccggtc gtgctcgcgc gcctgctcgg cgtgatcgac gaggtgagcc
     4201 gcgagctgcc ggtcgtgttt ccggtgcatc cgcgcacgca gggcaagatt cgcgaggccg
     4261 gcctcggtgc cgtgctcgac ggcacgcgct ggctggtgct gccgccggtc ggctacttcg
     4321 agatgctcgg cctgatgcgc gaggcgcggc tggtgctgac cgactcgggc ggcctgcagg
     4381 aggaaaccac ggcgctcggc gtgccctgcc tgacgctgcg cgagaacacc gagcgcccga
     4441 tcacgatcgc gcagggcacc aacacgctgg tcggcactga cgccgacgcg atccgtgccg
     4501 cggtgcgtga ggtgctcgcg accggcggca aggccgggcg cacgcccgag cgctgggacg
     4561 gccatgccgc cgggcgcatc gtcgctgatc tcgccgaatg gctcgctccg gcctgacggc
     4621 cgcgccgcct cccatcgccc ttttgcggaa ccgcagatca tgaagatcct cgtcaccggc
     4681 gccgccggct tcatcggctt ccatctgacg cagcgcctgc tcgcgcgcgg cgatgaagtc
     4741 atcgggctcg acaacctcaa tgattactac gacgtggcgc tcaagcaggc gcggctcgcg
     4801 cagctcgaag cgcagccggg cttccgcttc gtcggactcg atctggctga ccgcccgggc
     4861 atcgccgcat tgttcgaagc cgaacgcttc gcgcgcgtcg tcaacctcgc cgcgcaggcc
     4921 ggcgtgcgct attcgctgaa gaacccgcac gcctacgtcg acagcaac
//
LOCUS       DAOQJH010000094         4965 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_9995_length_4965_cov_3.803870, whole genome shotgun sequence.
ACCESSION   DAOQJH010000094 DAOQJH010000000
VERSION     DAOQJH010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 4965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4965
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9995_length_4965_cov_3.803870"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..359
                     /locus_tag="PLQ18_03365"
     CDS             <1..359
                     /locus_tag="PLQ18_03365"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017941755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNE00721.1"
                     /translation="LKRINDGSGHAAGDELICRAAGALRKAARTVDVVARLGGDEFGV
                     LCAECDGPHGRRLAGRLRTALREAGVDASIGIACRRPDAGIQAAWEQADQQMYEEKKT
                     RTGGGRDQPAGGRFAS"
     gene            complement(329..955)
                     /gene="mobA"
                     /locus_tag="PLQ18_03370"
     CDS             complement(329..955)
                     /gene="mobA"
                     /locus_tag="PLQ18_03370"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007193173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HNE00722.1"
                     /translation="MSEGIRDGVACSAGISAVILAGGRGSRMGGVDKGLIEVAGTALI
                     DRTLTALRPQVSTVLINANRNREAYARRGCPVLADATPDYDGPLAGMAAALAVIETPW
                     LLAVPCDCPQLAPDLAARLLAAAEAAGAELAVAHDGERLQPVFVLLHRDLRVPLAQYL
                     GGGGRKLEHWSRSRRMTEADFSDRPQTFVNLNSPDEKSAYEANRPPAG"
     gene            1150..2556
                     /locus_tag="PLQ18_03375"
     CDS             1150..2556
                     /locus_tag="PLQ18_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011758248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNE00723.1"
                     /translation="MSFCPVPAVPEPLPRLDALAVLVVDDEPGMCNFLCRALARHVAR
                     VEAAGSLEAARAARLAAHYDLLLVDQRLPDGSGVDWLAELRAAGAHEQLIVITAYADL
                     DTAIRALRAGAADFLLKPARLDQVLESVRRAAALLPQPGRPQPPAPVAELIGDSTAIR
                     DLSALIRRIAPHPSTLLIEGESGTGKECVARAVHRLSGRSGPYVPLNCGALPRELLES
                     ELFGHVRGAFSGALAGREGLFAHADGGTLFLDEISELPLGMQTALLRVLEDRRVRPLG
                     SERERAIDVRIITASNRDLAAEVAAGRFRADLYYRLNVLALRLPPLRERPADLAPLAQ
                     HFAQQLAGELGLPAPLLDAATLAALAAHDWPGNVRELRNLIERALLLGCPPAECLPQT
                     PAIAAAAASAASASAVVTAATGVPEPAPLALDAVEKRHILQVLDACGGNKSVAARRLG
                     IARKTLDRKLAAWQGRPS"
     gene            2597..4666
                     /locus_tag="PLQ18_03380"
     CDS             2597..4666
                     /locus_tag="PLQ18_03380"
                     /inference="COORDINATES: protein motif:HMM:NF028510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HNE00724.1"
                     /translation="MPVRLFGRYGHGIRHRLLLLVLLPSVLLLPMLLLTTLAWGERFS
                     QRQSLARVATDLSVAENVFARMQRDTLDALHRLADSWALRTALAGGTPVPMALLERDV
                     GGDGFGYVRLLDAGGRPRDGGPPPPPSKLLDAARAGQAASGIERFEAAALDAARDGLA
                     ALARVEHAGTQEPADERGLVVRAAVPVLDEQGAVLALLDGGVLLNRNYRPVDEIRDLV
                     YGPGTLLPGAIGTVTLFLDDLRVSTNVPLHAGERALGTRVSPEVRRQVLEHGERWLGR
                     AFVVNAWYLSAYAPLLDVHGERIGMLYTGFLEAPYRQAWDDAAGALIGACLLADALLG
                     ALALWLARSLFRPLERLLAVTRAVRSGATGARALPGPGRDELAELAREFDAMLDRLAQ
                     HEHELETAAQALERTVAERTEDLRRQNARLAETVAELAEARQRLLTTEQLAALGALAA
                     GVAHEINNPLAVLLGNLDVMVAELGEAAAPVQVEIDLAVAQVERIRRIVRGLLDYARP
                     QRQPLGGCVADLPALFEETLLLAGHSVQGGGVMIQRDWQARQAVRIGAPELQQVLLNL
                     IVNALQALAGRGRLWLTTADKQGRVVLVVADDGPGIEARDLPRVFDPFFTTRTSGTGL
                     GLAVSFAIVRRHGGEITIGTRPGGGCLVTVCLPALEAAGCKSGADSPSCALASPSPSL
                     SCRESDR"
     gene            4663..>4965
                     /locus_tag="PLQ18_03385"
     CDS             4663..>4965
                     /locus_tag="PLQ18_03385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE00725.1"
                     /translation="MKPLSLSPFALALVWQTLHARLSLRLALLAVAVFLTGTLADAPG
                     SASPSMAVWCLDAGLLALALRARRRHVPAYLLVSFLAAVAGRLAAGDGVWFALGFAV"
BASE COUNT          596 a         1812 c         1817 g          740 t
ORIGIN      
        1 gcctcaagcg catcaacgac ggttcggggc acgccgccgg cgacgagctg atctgccggg
       61 ccgccggggc gctgcgcaag gccgcgcgca cggtcgatgt ggtcgcgcgc ctcggcggcg
      121 acgaattcgg cgtgctgtgc gccgagtgcg atgggccgca cggccggcgg ctcgccggac
      181 ggctgcgcac ggcgctgcgc gaagccgggg tcgatgcctc gatcggcatc gcctgccgcc
      241 ggcccgacgc cggcatccag gccgcctggg aacaggccga tcagcagatg tacgaggaga
      301 agaagacccg cacgggcggc ggacgcgatc agccggccgg cggccggttc gcctcgtagg
      361 cggatttctc gtcgggtgag ttgaggttca cgaaggtctg cggccggtcg ctgaaatcgg
      421 cttcggtcat gcgccggctc cgggaccagt gctcaagctt gcgcccgccg ccgcccaggt
      481 attgcgcgag cggcacgcgc aggtcgcggt gcagcagcac gaacaccggc tgcaggcgct
      541 cgccgtcgtg cgcgaccgcg agttcggcac cggcggcttc ggcggcggcg agcaggcggg
      601 cggccagatc cggagcaagc tgcgggcagt cacagggcac ggccagcagc cacggcgttt
      661 cgatcaccgc cagcgccgcg gccatgccgg cgagcgggcc gtcgtagtcc ggcgtggcat
      721 cggcgagcac cgggcagccg cgccgcgcat aggcttcccg gttgcggttg gcgttgatca
      781 gcaccgtgga cacctgcggg cgcagcgcgg tgagcgtgcg gtcgatcagc gccgttccgg
      841 ccacctcgat caggcccttg tcgaccccgc ccatgcggct gccgcggccg ccggcgagga
      901 tcaccgcgct gatgccggca ctgcaggcga cgccatcacg gatgccctca ctcatgtctg
      961 cctctcatgc tcggtcccgc gcatgtctgc ggattccggc tgccggtgaa ggagggggca
     1021 gcatagcgac tgctgcgcgc tgcgcacgga cgctacttgg tgcggtgcag cgtgaattcg
     1081 ggcctgcatg gcccatacat tgccggcagg taatgcatgt ttcattcacg tccactgccg
     1141 gtgacgctca tgtccttctg ccccgtgccc gccgttccgg agccgttgcc gcgcctcgat
     1201 gcactggcgg tgctcgtcgt cgatgacgag cccggcatgt gcaacttcct ctgccgggcg
     1261 ctggcacgcc atgtcgcgcg ggtcgaagcg gccggcagcc tggaggcggc gcgtgcggcg
     1321 cgcctggcgg cgcactacga cctgctgctg gtcgaccagc gcctgccgga cggttcgggc
     1381 gtcgactggc tggcagaact gcgcgccgcc ggcgcgcacg agcaactgat cgtgatcacg
     1441 gcctatgccg atctcgacac cgcgatccgg gcgctgcgcg ccggtgccgc cgacttcctg
     1501 ctgaagccgg cgcggctcga tcaggtgctc gaatccgtgc gtcgcgccgc ggcgctgctg
     1561 ccgcagccgg gccggccgca gccgccggca ccggtcgcgg agctgatcgg cgacagcacg
     1621 gcgatccgtg atctgagcgc gctgatccgg cgcatcgcgc cgcatccctc gacgctgctg
     1681 atcgagggcg agagcggcac cggcaaggaa tgcgtcgcgc gcgccgtgca tcgcctgagc
     1741 gggcgcagcg gcccctacgt accgctcaac tgcggcgcgc tgccgcgcga gctgctggag
     1801 agcgagctgt tcggacacgt gcgcggtgcc ttcagcggcg cgctggccgg gcgcgagggc
     1861 ctgttcgcgc acgccgacgg cggcacgctg ttcctcgacg agatcagcga gctgccgctc
     1921 ggcatgcaga cggcgctgct gcgcgtgctg gaggaccggc gcgtgcgccc gctcggcagc
     1981 gagcgcgagc gcgcgatcga cgtgcgcatc atcaccgcca gcaaccgcga tctggccgcc
     2041 gaggtcgccg ccgggcgttt ccgcgccgat ctgtactacc gtctgaacgt gctggcgctg
     2101 cgtctgccgc cgctgcgcga gcgcccggcc gacctcgcgc cgcttgcgca gcatttcgcg
     2161 cagcagctcg ccggcgaact cggcctgccg gcgccgctgc tcgatgccgc gacgctggcg
     2221 gcgctggccg cgcacgactg gcccggcaac gtccgtgagc tgcgcaacct catcgaacgc
     2281 gcgctgctgc tcggctgccc gccggccgag tgcctgccgc agacgccggc catcgccgcc
     2341 gccgctgcaa gcgcggccag cgcgagcgcg gtcgtgacgg ccgcgacagg cgtgccggag
     2401 ccggcgccgc ttgcgctcga tgccgtcgag aagcgccaca tcctgcaggt gctcgatgcc
     2461 tgcggcggca acaagtccgt ggccgcgcgc cgcctcggca tcgcccgcaa gaccctggat
     2521 cgcaagctcg ccgcctggca ggggcgtcca tcctgagcgc gggtgtgccg ctgccgatgc
     2581 cccggccatc cgcctgatgc cggtacgcct gttcggccgc tacggccacg gcatccgcca
     2641 tcgcctgctg ctgctggtgc tgctgccgag cgtgctgctg ctgccgatgc tgctgctgac
     2701 gacgctcgcc tggggcgagc gcttcagcca gcgtcagtcg ctggcgcgcg tcgcgaccga
     2761 cctgtcggtg gccgagaacg tcttcgcgcg catgcagcgc gacacgctcg atgcgctgca
     2821 ccggctcgcg gattcctggg cgctgcgcac ggcgctcgcc ggcggcacgc cggtgcccat
     2881 ggcgctgctg gagcgcgatg tcggcggtga tggcttcggc tacgtgcgcc tgctcgatgc
     2941 cggcggcagg ccgcgcgacg gcggtccgcc gccgccgccg tcgaaactgc tcgatgccgc
     3001 gcgcgccgga caggcggcaa gcggcatcga gcgcttcgag gccgcggccc tcgatgccgc
     3061 gcgcgacggt ctggccgcgc tcgcgcgcgt cgagcacgcc ggcacccagg agccggccga
     3121 tgagcgcggg ctggtcgtgc gcgccgcggt gccggtgctc gatgagcagg gcgcggtgct
     3181 ggcgctgctc gatggcggcg tgctgctgaa ccgcaactac cggccggtcg acgagatccg
     3241 cgatctcgtc tacggccccg gcacgctgct gccgggcgcg atcggcacgg tgacgctgtt
     3301 tctcgatgac ctgcgcgtgt cgaccaacgt gccgctgcac gccggcgagc gggcgctcgg
     3361 cacccgcgtc tcgcccgagg tgcgccgcca ggtgctggag cacggcgagc gctggctcgg
     3421 gcgcgccttc gtcgtcaacg cctggtatct gtcggcctac gcgccgctgc tcgacgtcca
     3481 cggcgagcgc atcggcatgc tctacaccgg ctttctcgaa gctccgtacc ggcaggcctg
     3541 ggacgatgcc gccggcgcgc tgatcggcgc ctgcctgctc gccgatgcgc tgctcggggc
     3601 gctggcgctg tggctggcgc gttcgctgtt ccggccactg gagcgcctgc tcgccgtgac
     3661 ccgcgccgtg cgcagcggtg cgaccggtgc ccgtgccctg cccggacccg ggcgcgatga
     3721 actcgccgag ctggcccgcg agttcgatgc gatgctcgat cggctcgcgc agcacgagca
     3781 cgaactcgaa accgcggcgc aggcgctgga gcgcacggtc gccgaacgca ccgaggacct
     3841 gcgccgccag aacgcccgac tggccgagac cgtcgccgag ctggccgagg cgcgccagcg
     3901 cctgctgacc accgagcagc tcgccgcgct cggcgcgctc gccgccggcg tcgcgcacga
     3961 gatcaacaat ccgctggccg tgctgctcgg caacctcgat gtcatggtcg ccgaactcgg
     4021 cgaggccgcg gccccggtgc aggtcgagat cgacctcgcc gtcgcgcagg tcgagcgcat
     4081 ccgccgcatc gtgcgcggcc tgctcgatta cgcccggccg cagcgccagc cgctcggcgg
     4141 ctgtgttgcc gatctgccgg cgctgttcga ggaaaccctg ctgctcgccg gccacagcgt
     4201 gcagggcggc ggggtcatga tccagcgtga ctggcaggcc cgtcaggccg tgcgcatcgg
     4261 cgcacccgag ctgcagcagg tgctgctgaa cctgatcgtc aacgcgctgc aggcactcgc
     4321 cggccgcggc cggctgtggc tgacgacggc cgacaagcag ggccgcgtcg tgctggtcgt
     4381 ggccgacgac ggaccgggca tcgaggcccg ggatctgccg cgggtgttcg atccgttctt
     4441 cacgacccgc accagcggca ccgggctcgg actggccgtc agtttcgcga tcgtgcgtcg
     4501 ccatggcggc gagatcacga tcggcacgcg accgggcggg ggctgtcttg tgacagtctg
     4561 tttgccggca ctcgaggctg ccggctgcaa gagcggcgcc gattcgccat catgcgccct
     4621 ggcgtcgcca tcgccgtccc tgtcctgccg cgaatcagac cgatgaagcc gttgtccctg
     4681 tcgcccttcg cgctcgccct tgtctggcag acgctgcatg cgcggctgtc cctgcggctg
     4741 gcgctgctgg cagtcgcggt gttcctgacc ggcactcttg ccgatgcgcc cggcagtgca
     4801 agtccgtcga tggcggtctg gtgcctggat gcagggctgc tggcgctggc gctgcgcgcc
     4861 cggcgcaggc acgtgcctgc gtatctgctc gtcagcttcc tggccgcggt ggcgggccgg
     4921 ctcgccgccg gtgatggcgt ctggttcgcg ctcggctttg cggtg
//
LOCUS       DAOQJH010000095         4949 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_10048_length_4949_cov_3.933183, whole genome shotgun sequence.
ACCESSION   DAOQJH010000095 DAOQJH010000000
VERSION     DAOQJH010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 4949)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4949)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4949
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10048_length_4949_cov_3.933183"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..710)
                     /gene="phbB"
                     /locus_tag="PLQ18_03390"
     CDS             complement(<1..710)
                     /gene="phbB"
                     /locus_tag="PLQ18_03390"
                     /EC_number="1.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018454 - acetoacetyl-CoA reductase
                     activity [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetyl-CoA reductase"
                     /protein_id="HNE00726.1"
                     /translation="MARVAVVTGGMGGLGEAICIKLAALGDKVVATYSPGNKTAEEWL
                     AGMKAQGFDFSAVPVDVTDFDSCAACVKQVEADIGPIDVLVNNAGITRDMTFKKMTKA
                     DWDAVIKTNLDSVFNMTKQVMDGMMARNWGRVVNISSVNGIKGAFGQTNYAAAKAGMH
                     GFTKALALEVCKKGVTVNTISPGYIGTKMVRAIPQEVLDTKILPLIPMGRLGEPNEVA
                     GLVAYLCSEEAAFLTGAN"
     gene            1097..2299
                     /gene="ilvA"
                     /locus_tag="PLQ18_03395"
     CDS             1097..2299
                     /gene="ilvA"
                     /locus_tag="PLQ18_03395"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012527511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006549 - isoleucine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HNE00727.1"
                     /translation="MISLDDIIKARRRIRDSLPPTPCARAAQLSRQLGCELFIKCENL
                     QMTGSFKVRGALARILQLTEAERAAGVIAASAGNHAQGVAWAAQKFGVPATIVMPETA
                     PLAKIRGTAAFGADIVLAGTGYDAAYARAVELQRDSGATFIHAFDDPAVMAGQGSAGL
                     EIIEQLGDFDLVLVPVGGGGLAGGIATVIKQTRPGVQVIGVQSARVPSMQASLAAGAP
                     TPVPPAATLADGIAVARVGVHTYPQAAAWLDGIVTVDEESIARAILTLLEQEKLLAEG
                     AGAVGIAALLDGVVGDPAGKRIVAVVSGGNIDPIELAKIVERGLERDGRLAHLRVVVA
                     DRPGSIAEVAGLVARERANILQISQRRDAAEVGLREAELDLTLETRGLDHVTGIVQAL
                     RDGGFRVR"
     gene            2364..3872
                     /locus_tag="PLQ18_03400"
     CDS             2364..3872
                     /locus_tag="PLQ18_03400"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNE00728.1"
                     /translation="MSDSVPAPLTAAEVRAVVLGSVLALFLAALDQSIVATALPAIAA
                     ELGRVELMSWVVTAYLLASTCATPVLGKASDVLGRRRVLAGCLIVFLGGSVLCSLATS
                     MPMLIAARALQGLGGGGLITLAQATVADVVPPRERGRYVAYFASVWACAGLLGPTLGG
                     LLTQYLGWPWIFWINLPLGLAALVITDRVLRRLPVRGGTRGRIDLPGIVLLSTATTAL
                     LLALSFGGHRWPWASAPILGLLALAVVPALLFAHRQRHTAEPLLPPAFFRDPVVAPTG
                     AALFLVFGGFLAVAVLVPAWLQIGLGRSAAAAGLLMMPMSLAAPLTATLTGHYLRATG
                     RYRRPPQLGLPLAAVALLALAGVALAGPQAPAPGWLAALLALIGLGIGPIFSASLTAA
                     QNAVEPRDVGAVTGALTFARALGGAVWVAAGSALVLGLALAWLPQLGTLGGLEDLVRR
                     PLAAAERAALGEAFAAVFVAAAGLLLLGLLLWSRVAERPLRGADAAPVSAET"
     gene            complement(3891..4523)
                     /locus_tag="PLQ18_03405"
     CDS             complement(3891..4523)
                     /locus_tag="PLQ18_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009560708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HNE00729.1"
                     /translation="MNRLPLTLYMTPGSCSTGIHILLEELGVPFAAHIVNLPKGEHRQ
                     PAYLAINPRGTIPALRLEDGSALAGFTAIAYWLARRFPKAGLLPAEETAVARVLEALD
                     YVTGTIHGQGFTRIFTTDAYTTDPALQPAVQARGREIVAEGFALLAVQLGGRDWLVGE
                     TFSIADAALFYVEFWADHLGLPLPAHCRAHFERLRARPAVQQVLREEGYR"
     gene            complement(4615..>4949)
                     /locus_tag="PLQ18_03410"
     CDS             complement(4615..>4949)
                     /locus_tag="PLQ18_03410"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018233492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HNE00730.1"
                     /translation="RAHDDALVWPGLQDLTAHVDFTAVADAALAAGFEVAGYCAQAWF
                     LFGCGLEALLAEVDPEDLPRHVEAVRQVKLLTLPGEMGERFQAIGLTKGIGLRLPAFA
                     LRDERGRL"
BASE COUNT          644 a         1770 c         1752 g          783 t
ORIGIN      
        1 atgttggcgc cggtcaggaa cgccgcttcc tcggagcaca gatacgcgac cagacccgcg
       61 acctcgttcg gctcgccgag gcggcccatc gggatcagcg gcaggatctt ggtgtcgagc
      121 acttcctgcg ggatggcgcg gaccatcttg gtgccgatgt agcccggcga gatggtgttg
      181 acggtcacgc ccttcttgca gacctcgagc gccagtgcct tcgtgaagcc atgcatgccg
      241 gccttggccg ctgcgtagtt ggtctggccg aaggcgccct tgatgccgtt gaccgacgag
      301 atgttgacga cgcgccccca gttgcgagcc atcatgccgt ccatgacctg cttggtcatg
      361 ttgaacacgg aatccaggtt ggtcttgatg accgcgtccc agtcggcctt ggtcatcttc
      421 ttgaaggtca tgtcgcgggt gatgccggcg ttgttgacca gcacgtcgat cggaccgatg
      481 tcggcctcga cctgcttgac gcaggccgcg caggagtcga agtcggtgac atcgaccggc
      541 actgccgaga agtcgaagcc ctgcgccttc atgcccgcga gccattcctc ggcggtcttg
      601 ttgcccggcg aataggtcgc cacgaccttg tcccccagcg ccgcgagctt gatgcagatc
      661 gcctcgccga gtccccccat gccgccggtc actacagcca ctcgtgccat tgcaaaacct
      721 cctcgttcga attcagcaca tgaacccggc cgctcctttg cgggcacgaa cgtgacctgt
      781 gaggcaggcg aacgccccgt gcggatcggc gggccggttt ttgtggtgct tctttaaaag
      841 tgtttcctgt cgcgtttcgg gcatgcggca gcggttcctg ccggcatgac tgctttgcac
      901 gcgtgcaccg ctcgtgacgg taccgccccc ctgctgttat gcgacccggc tagtatgttc
      961 tcgcccggac gggatgccaa gccacgcggc gcacccgatt gaccggaatc aagcgcttga
     1021 tgagaaaagg atttctactc atgcgccacg tgaaatgaac tattcttcat ttcttataac
     1081 gggatttgaa atcatcatga tttcacttga tgacatcatc aaggctcgcc ggcgcatccg
     1141 cgacagcctg ccgccgacgc cgtgcgcccg cgccgcgcag ttgtcgcgcc aactcggctg
     1201 cgagctgttc atcaagtgcg aaaacctgca gatgaccggc tcgttcaagg tgcgcggcgc
     1261 gctcgcgcgc atcctgcagc tgaccgaggc cgagcgcgcc gccggcgtca tcgccgcctc
     1321 ggccggcaac cacgcgcagg gcgtggcctg ggcggcgcag aaattcggcg tgccggcgac
     1381 catcgtcatg ccggaaaccg cgccgcttgc gaagatccgc ggcactgccg ccttcggggc
     1441 cgacatcgtg ctcgccggca ccggctacga cgccgcctat gcgcgcgccg tcgaactgca
     1501 gcgcgacagc ggcgcgacct tcatccacgc cttcgacgac ccggcggtga tggccggcca
     1561 gggctcggcg gggctggaga tcatcgagca gctcggcgat ttcgatctgg tgctggtgcc
     1621 ggtcggcggc ggcggcctcg ccggcggcat cgccaccgtc atcaagcaga cccggcccgg
     1681 cgtgcaggtg atcggcgtgc agagcgcgcg cgtgccgtcg atgcaggcga gtctcgccgc
     1741 cggcgcgccg acgccggtgc cgccggcggc gacgctcgcc gacggcatcg cggtcgcgcg
     1801 tgtcggcgtg cacacctatc cgcaggccgc tgcctggctc gacggcatcg tcaccgtcga
     1861 cgaggaatcg atcgcgcgcg cgatcctgac cctgctcgaa caggaaaagc tgctggccga
     1921 aggcgccggc gcggtcggca tcgcggcgct gctcgatggc gtcgtcggcg acccggccgg
     1981 caagcgcatc gtcgccgtgg tctcgggcgg caacatcgac ccgatcgaac tcgcgaagat
     2041 cgtcgagcgc gggctggagc gcgacgggcg gctcgcgcac ctgcgcgtcg tcgtcgccga
     2101 ccggcccggc tcgatcgccg aggtcgccgg cctggtcgcg cgcgagcgcg ccaacatcct
     2161 gcagatcagc cagcgccgtg acgccgccga ggtcggcctg cgcgaggccg aactcgacct
     2221 gacgctggaa acccgcgggc tcgatcacgt caccggcatc gtgcaggcgc tgcgcgacgg
     2281 cggattccgc gtacgctgac gaaccgccgc cgttcgcgcg ggtccagatt ccgtcgcccc
     2341 gcctcatccg ccggtatccc gagatgtccg attccgtccc cgcgccgctg accgctgccg
     2401 aggtccgcgc cgtcgtgctc ggctcggtgc tggcgctgtt cctcgccgcg cttgaccaga
     2461 gcatcgtcgc cacggcgctg ccggcgatcg ccgccgagct cggccgggtc gagctgatgt
     2521 cctgggtggt caccgcctac ctgctcgcct cgacctgcgc caccccggtg ctcggcaagg
     2581 cgagcgacgt gctcgggcgc cggcgcgtgc tcgccggctg cctgatcgtg ttcctcggcg
     2641 gctcggtgct ctgctcgctc gcgacttcga tgccgatgct gatcgcggcg cgcgcgctgc
     2701 agggcctcgg cggcggcggg ctgatcacgc tggcgcaggc gacggtcgcc gacgtggtgc
     2761 cgccgcgcga gcgcgggcgc tacgtcgcct acttcgcgtc ggtctgggcc tgcgccggcc
     2821 tgctcggacc gacgctcggc gggctgctga cgcaatacct gggctggccg tggatcttct
     2881 ggatcaacct gccgctcgga ctcgcggcgc tcgtgatcac cgaccgcgtg ctgcgccggc
     2941 tgccggtgcg cggcggcacg cgcgggcgca tcgacctgcc cggcatcgtg ctgctgtcga
     3001 cggcgacgac ggcgctgctg ctggcgctga gcttcggcgg ccaccgctgg ccgtgggcct
     3061 cggcgccgat cctcggcctg ctggcgctcg ccgtcgttcc ggcgctgctg ttcgcgcacc
     3121 gccagcggca cacggccgag ccgctgctgc cgccggcctt cttccgcgat ccggtggtcg
     3181 caccgaccgg cgcggcgctg ttcctggtgt tcggcggctt cctcgcggtc gcggtgctgg
     3241 tgccggcctg gctgcagatc ggcctcggca ggagcgccgc cgcggccggg ctgctgatga
     3301 tgccgatgtc actggccgcg ccgctgaccg cgacgctgac cggccactac ctgcgcgcga
     3361 ccggccgcta ccgccggccg ccgcagctcg gcctgccgct ggcggcggtg gcgctgctgg
     3421 cgctggccgg ggtcgcgctg gccgggccgc aggcaccggc tccgggctgg ctcgcggcgc
     3481 tgctggcgct gatcgggctc ggcatcggcc cgatcttctc ggcgagcctg accgccgcgc
     3541 agaacgcggt cgagccgcgc gatgtcggcg ccgtcaccgg cgcgctgacc ttcgcgcgcg
     3601 ccctcggcgg cgcggtctgg gtcgcggccg gatcggcgct ggtgctcggg ctggcgctcg
     3661 cgtggctgcc gcagctcggc acgctcggcg gccttgaaga cctcgtgcgc cggccgctgg
     3721 ccgctgccga gcgcgccgcg ctcggcgagg ccttcgccgc ggtgttcgtg gctgcggccg
     3781 ggctgctgct gctcggcctg ctgctgtggt cgcgcgtagc cgaacggccg ctgcgcgggg
     3841 cggacgctgc gccggtcagc gccgaaacct gaaccgggca gcgcaccggc tcagcgatag
     3901 ccttcctcgc gcagcacctg ctgcacggcc ggccgcgcgc gcaggcgctc gaagtgcgcg
     3961 cggcagtgcg ccggcagcgg caggccgagg tgatcggccc agaactcgac gtagaacagc
     4021 gcggcatcgg cgatcgagaa ggtctcgccg accagccagt cgcggccgcc gagctgtacc
     4081 gcgagcagcg cgaagccttc ggcgacgatc tcgcgcccgc gcgcctgcac ggccggctgc
     4141 agcgccggat cggtcgtgta ggcatcggtc gtgaagatgc gcgtgaagcc ctgaccgtgg
     4201 atcgtgccgg tcacgtagtc gagcgcttcc agcacccgcg ccacggcggt ttcttcggcc
     4261 ggcagcagcc cggccttcgg gaagcgccgc gccagccagt acgcgattgc ggtgaagccg
     4321 gcgagcgcgc tgccatcttc gaggcgcagc gccggaatcg tgccgcgcgg gttgatcgcc
     4381 agatacgccg gctggcgatg ctcgcccttc ggcaggttga cgatgtgcgc ggcgaagggc
     4441 acgccgagct cttcgagcag gatgtggatg ccggtcgagc acgaaccggg cgtcatgtac
     4501 agcgtcagcg gcaaacggtt catggcggtc accggtagcg ggagtcaggg ggacgatcgg
     4561 aggcggcggc agcgacggcg agcgccccga gggacggcag gcatgcgccc ggcctcacag
     4621 ccgcccccgc tcgtcgcgca gcgcgaacgc cggcagacgc aggccgatac ccttcgtcag
     4681 cccgatcgcc tggaagcgct cgcccatctc gccgggcagc gtcagcagct tgacctgacg
     4741 cacggcctcg acgtggcgcg gcaggtcttc gggatcgacc tcggcgagca gcgcttccag
     4801 gccgcagccg aacaggaacc aggcctgcgc gcagtagccg gcgacctcga agccggcggc
     4861 gagcgcggca tcggcgacgg cggtgaaatc gacgtgcgcg gtcaggtcct gaagccccgg
     4921 ccagaccagc gcgtcgtcgt gggcgcggt
//
LOCUS       DAOQJH010000096         4924 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_10121_length_4924_cov_4.470322, whole genome shotgun sequence.
ACCESSION   DAOQJH010000096 DAOQJH010000000
VERSION     DAOQJH010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 4924)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4924)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4924
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10121_length_4924_cov_4.470322"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..491)
                     /locus_tag="PLQ18_03415"
     CDS             complement(<1..491)
                     /locus_tag="PLQ18_03415"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HNE00731.1"
                     /translation="MQHRILFLCLGSLCALPAAADICSYRDAQGVLHISNVCDGDSRY
                     RVTMATPSYANRPDAPSDYRPATAQLPPGVSLGHLPTTRVASSARRKLYSPAIHAVAA
                     QYRLEPALLHAVITAESAYDPLAVSRAGAMGLMQLMPATARRFGVSDAFDPTENLHGG
                     ARYL"
     gene            704..2254
                     /gene="pap"
                     /locus_tag="PLQ18_03420"
     CDS             704..2254
                     /gene="pap"
                     /locus_tag="PLQ18_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0043751 - polyphosphate:AMP
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006797 - polyphosphate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate:AMP phosphotransferase"
                     /protein_id="HNE00732.1"
                     /translation="MFRTAELDHSLDKTEYLRREAELRQELLTLQGELREADFPVLLV
                     FAGVDGAGKGETVNQLNEWLDTRLLLTRAFDQPSDEERERPLYWRYWRELPARGRIGM
                     YLSSWYSAPLLDRVYERIGVEIFDERLDRIRNFEEAIAHDGALILKFWMHLSAPAQEK
                     RLKKLERDPLLKWRVTARDWEHWRLYPRFIAAAERALMRTGTGDAPWKIVEGFDERYR
                     IVTVATILRDAIRRRLAERRLLHEVVQQVNDGGGETLQALVEGSAVLPGQPTVLDSLD
                     LGKALPKAEYRERLLKLQGRLNRLQRRARERGVSTVLVFEGWDAAGKGGAIRRITSAI
                     DARDYQVVQVAKPTDEEAARHYLWRFWRYMSRAGRMTIFDRSWYGRVLVERVEGFARP
                     AEWRRAYAEINDFEDHLLEHGIVLVKFWLHLSRGEQQRRFETRAATPWKSWKLTDEDW
                     RNRERWQDYEHAVNDMVEHTSTRQAPWVLVEAEDKYHARVCVLEHVCEHLERGLKRAA
                     RRNGTSAG"
     gene            complement(2345..3022)
                     /locus_tag="PLQ18_03425"
     CDS             complement(2345..3022)
                     /locus_tag="PLQ18_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002686191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HNE00733.1"
                     /translation="MNSYQPLPVGARTGAPSTVQGHRVLRNTYLLLSMTLLFSAATAG
                     FAVVTQAPPMHWLLMLVGMFGLLFLTMKLRNSAWGLASVFAFTGFMGYTLGPVLSRYL
                     ALPGGGELVMGAMGLTGLIFLSLSAYVLVSRRDFSFMGGFLFVGLIVALVASLAAMFF
                     SVPGLHVAVSAMVVMLMSGFILYDTSSILHGGETNYIMATVALYLDIFNLFTSLLQLL
                     GFLGSDD"
     gene            3256..3525
                     /locus_tag="PLQ18_03430"
     CDS             3256..3525
                     /locus_tag="PLQ18_03430"
                     /inference="COORDINATES: protein motif:HMM:NF024901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HNE00734.1"
                     /translation="MSIHGRMQALLAGLLLIASNGAWAQVFKCVDAAGLTSYSQTPCT
                     ADAREVGRWQGRVGAVMIPAGAAAVTMRPVDVNRTKLPVQAPSQP"
     gene            complement(3536..4660)
                     /gene="zapE"
                     /locus_tag="PLQ18_03435"
     CDS             complement(3536..4660)
                     /gene="zapE"
                     /locus_tag="PLQ18_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009721992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HNE00735.1"
                     /translation="MPYSRYQQDLKRPGFVHDPAQEHAVRLLQKLHDALIARPQPAAV
                     PVRSGGGLFGKLLGKSRETDAPTAPAIRGLYFWGGVGRGKTYLVDTFYDTLPFREKQR
                     LHFHRFMQMVHARLKDVKHQQDPLKIVAAQLAENLRVLCLDEFFVADITDAMLLHGLL
                     AALFERGVVLVTTTNIPPDLLYRDGLQRARFLPAIELLKTHCEVVNVDGGTDYRLRYL
                     ERAEIYHHPLDARAETVLAETFRSLAPEPGQHNARLEIEGRFLRTRELADGVVWFDFA
                     EICDGPRSQNDYIEIARQFHTVLIGRVPVLDWTMENAARRFLNLVDEFYDRNVKLILA
                     AAAPCEAIYTGEKLKFEYQRTVSRLQEMQSKEFLARPHLP"
     gene            complement(4729..>4924)
                     /locus_tag="PLQ18_03440"
     CDS             complement(4729..>4924)
                     /locus_tag="PLQ18_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008211333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HNE00736.1"
                     /translation="GPTSEWDTAASQILLEEAGGGLCDGARRPLRYNARHTLINPDFL
                     AFGDAGHDWTRYLPRRSPPA"
BASE COUNT          846 a         1567 c         1765 g          746 t
ORIGIN      
        1 aggtaacggg ctccgccatg caggttctcg gtcggatcga aggcatcgct gacgccgaaa
       61 cgcctggcgg tcgccggcat cagctgcatc aggcccatgg cgccggcacg cgacacggcc
      121 agcggatcgt aggcggattc ggcggtgatc accgcatgca gcagcgccgg ttcgagccgg
      181 tactgggctg cgacggcatg gatggccggg ctgtagagct tgcggcgggc cgagcttgcg
      241 acgcgcgtgg tcggcagatg accgaggctg acgccgggcg gcagctgtgc ggtcgccggc
      301 cgatagtcgg acggtgcgtc ggggcgattg gcgtagcttg gcgtcgccat cgtcacgcgg
      361 tagcgcgagt cgccgtcaca gacgttgctg atgtgcagca cgccctgggc gtcgcgatag
      421 gagcagatgt cggccgctgc cggcagggca cacagactgc cgagacacag gaaaagtatc
      481 cggtgctgca tggtgcgagg ttcatccggc tgcgacgttt gccgcggacg ggtgtccggg
      541 gcgccgtcta ttctggtcgg ggcgcccggc ggggggccgg ggctacggca gcagcaaagt
      601 actggatggc ggcagcggac acaaccgtct gtcgccattg aggttcgggc gccgttcatt
      661 tcctctgagg gtccgcgtcc ccggcgggcc atggcggaca gcgatgttca gaaccgcgga
      721 actcgatcat tccctggaca agaccgaata cctgcgccgt gaggccgagc tgcgtcagga
      781 gctgctgacg ctgcagggcg aactgcgcga ggcggatttt ccggtcctgc tggtcttcgc
      841 cggcgtcgac ggtgccggca agggggagac cgtcaaccag ctcaacgaat ggctggacac
      901 gcgcctgctg ctgacgcgcg ccttcgacca gccttcggac gaggagcgcg aacggccgct
      961 gtactggcgc tactggcgcg agctgccggc gcgcgggcgc atcggcatgt acctcagttc
     1021 gtggtactcg gcgccgctgc tcgatcgggt ctacgagcgc atcggtgtcg agatcttcga
     1081 cgagcgcctc gaccgcatcc gcaacttcga ggaagccatc gcgcacgacg gggcgctgat
     1141 cctgaagttc tggatgcatc tttcggcacc ggcgcaggag aagcggctga agaagctcga
     1201 aagggacccg ctgctgaaat ggcgggtcac ggcccgcgac tgggagcact ggcgcctgta
     1261 cccgcgcttc atcgccgctg ccgagcgggc gctgatgcgc accggcaccg gcgatgcgcc
     1321 gtggaagatc gtcgaggggt tcgacgagcg ctaccgcatc gtcacggtcg cgaccatcct
     1381 gcgggatgcg atccgccgcc ggctcgccga gcggcgcctg ctgcacgaag tcgtgcagca
     1441 ggtgaacgat ggcggcggcg agacgctgca ggcgctggtc gagggcagcg ccgtgctgcc
     1501 cggacagccg accgtgctcg attccctcga tctcggcaag gccttgccga aagccgagta
     1561 ccgcgaacgc ctgctgaagc tgcagggacg cctcaaccgc ctgcagcgcc gggcgcgcga
     1621 gcgcggcgta tcgacggtgc tggtgttcga gggctgggat gccgccggca agggcggtgc
     1681 gatccggcgc atcaccagcg ccatcgatgc gcgcgactat caggtcgtgc aggtcgccaa
     1741 gccgaccgac gaggaggccg cgcggcatta tctgtggcgg ttctggcggt acatgagccg
     1801 cgccgggcgc atgaccatct tcgaccgctc ctggtacggc cgcgtgctgg tcgagcgcgt
     1861 cgagggcttc gcccgaccgg cggagtggcg gcgcgcctac gccgagatca acgacttcga
     1921 ggatcacctg ctcgaacacg gcatcgtgct ggtgaaattc tggctgcatc tgagccgcgg
     1981 ggagcagcag cggcgcttcg agacacgggc cgcgacgccg tggaagtcct ggaagctcac
     2041 cgacgaggac tggcgcaacc gcgagcgctg gcaggactac gagcatgccg tcaacgacat
     2101 ggtcgagcac accagtaccc gccaggcgcc gtgggtgctg gtcgaggccg aggacaagta
     2161 ccacgcgcgc gtctgtgtgc tcgaacacgt ctgcgagcat ctcgaacgcg gtctcaagcg
     2221 cgccgcccgg cgcaacggga cttcggccgg ctgaggaggc cggccgggct ccggcgcggg
     2281 ccggccagaa aaaagccccg ctgcagcggg gcttgactgg ctacgacccg tgccgcagca
     2341 gcgatcagtc gtcgctgccg agaaagccga gcagctgcag caggctcgtg aacaggttga
     2401 agatgtccag atacagcgcg acggtcgcca tgatgtagtt ggtctcgccg ccgtgcagga
     2461 tcgaactggt gtcgtacagg atgaagcccg acatcagcat cacgaccatc gccgaaaccg
     2521 caacgtgcag gcccggcacg gagaagaaca tcgccgcgag gctcgcgacc agcgcgacga
     2581 tcaggccgac gaacaggaat ccgcccatga agctgaagtc tcggcgcgac accagcacgt
     2641 aggccgacag cgacaggaag atcaggccgg tcaggcccat cgcccccatg accagttcgc
     2701 cgccacccgg cagcgccagg taacgcgaca gcaccgggcc gagcgtgtag cccatgaagc
     2761 cggtgaacgc gaacaccgag gccaggcccc aggcgctgtt gcgcagcttc atcgtcagga
     2821 acagcaggcc gaacatcccg accagcatca gcagccagtg catcggcggc gcctgcgtga
     2881 cgacggcgaa accggcggtg gcggcgctga acagcagcgt catcgacagc agcaggtagg
     2941 tgttgcgcag gacgcgatgg ccctgcaccg tggacggcgc accggtgcgg gcgccgaccg
     3001 gcagcggttg atacgagttc atcgtcggtg acctccgggg atgatcgacg tggcaatgga
     3061 ccggccggac agcgaccggt tccagcgaca cattgatatt acctgtaaga aaaaggtctg
     3121 caagtccgga aaccttacga ttcttaatca aggttaagaa ttgtctgagc tgcgagtccg
     3181 aagcgcggct gcagaccgat gccctgccag gagccagcat agacggctgg tccacgtacc
     3241 tgatcggaag ctgccatgtc gattcacgga agaatgcagg ccctgctggc cggcctgctg
     3301 ctgattgcca gcaacggggc atgggcgcag gtgttcaagt gcgtcgatgc cgccggcctg
     3361 acgagctaca gccagacgcc ctgtaccgcc gatgcccggg aagtcggccg ctggcagggc
     3421 cgggtcggcg cggtgatgat tccggcggga gccgcggccg tcacgatgcg cccggtcgac
     3481 gtgaaccgca cgaagctgcc ggtgcaggct ccttcgcagc cgtgaggcgc tgccctcagg
     3541 gcaggtgcgg gcgggccaga aattccttgg actgcatctc ctgcagccgg ctgaccgtgc
     3601 gctggtactc gaacttcagc ttctcgccgg tgtagatcgc ttcgcacgga gcggccgcgg
     3661 cgaggatcag cttgacgttg cggtcgtaga actcgtcgac gaggttcagg aagcgccgcg
     3721 cggcgttttc catggtccag tccagcaccg gcacccggcc gatcagcacg gtgtggaact
     3781 gccgggcgat ctcgatgtag tcgttctgcg agcgcggccc gtcgcagatt tccgcgaagt
     3841 cgaaccagac cacgccgtcg gccagttcgc gcgtgcgcag gaagcgaccc tcgatctcca
     3901 gccgggcatt gtgctggccc ggctccgggg ccagggagcg gaaggtctcg gccagcacgg
     3961 tctcggcgcg cgcatcgagc gggtggtggt agatctcggc gcgctccaga tagcgcagcc
     4021 ggtaatcggt gccgccatcg acgttgacga cctcgcagtg cgtcttcagc agctcgatcg
     4081 ccggcaggaa gcgcgcgcgc tgcaggccgt ccctgtacag caggtcgggc gggatgttgg
     4141 tggtcgtgac cagcaccacg ccgcgctcga acagcgccgc gagcaggccg tgcagcagca
     4201 tcgcgtcggt gatgtcggcg acgaagaact cgtcgagaca gagcacgcgc aggttctcgg
     4261 caagctgcgc ggcgacgatc ttcagcggat cctgctggtg tttcacgtcc ttcagccgcg
     4321 catgcaccat ctgcatgaag cgatggaagt gcaggcgctg cttctcgcga aacggcagcg
     4381 tgtcgtagaa ggtgtcgacc agataggtct tgccgcgccc gactccgccc cagaagtaca
     4441 ggccgcggat cgccggcgcg gtcggcgcat cggtttcccg gctcttgccg agcagcttgc
     4501 cgaacaggcc gccgccgctg cgcaccggca cggccgcagg ctgcggccgg gcgatcaggg
     4561 catcgtgcag cttctgcagc aggcgcacgg cgtgttcctg ggccggatcg tgcacgaagc
     4621 ccgggcgctt caggtcctgc tggtaacggg agtacggcat ggcgatggct cggacggcag
     4681 ggatgcggca gtgcggcatt gtgcgcagcc gccaggggca gcggcaagtc aggccggggg
     4741 gctgcgacgc ggcagatagc gggtccagtc gtggccggcg tcgccgaagg ccaggaaatc
     4801 cgggttgatc agcgtgtgcc gggcgttgta gcgcagtggt cggcgtgcgc cgtcgcagag
     4861 cccgccaccg gcttcctcca gcaggatctg cgaggctgcc gtgtcccatt cgctggtcgg
     4921 gccg
//
LOCUS       DAOQJH010000097         4920 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_10133_length_4920_cov_4.287359, whole genome shotgun sequence.
ACCESSION   DAOQJH010000097 DAOQJH010000000
VERSION     DAOQJH010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 4920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4920
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10133_length_4920_cov_4.287359"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1611)
                     /locus_tag="PLQ18_03445"
     CDS             complement(<1..1611)
                     /locus_tag="PLQ18_03445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNE00737.1"
                     /translation="MTALRPPGPAAPFARLAEHLPDPVFACNSDGSVLAYNPAAAGLA
                     GTVGLDAPEQLLPARHVEYVHECLYHDEIAELADTRVGPFTLCWTYVPSGGGTAVHVL
                     GRDVTDSRRTEAALRISEARHRLLAEQSTDIISRHSPGTWTFLYASPAAQTLLGYAPE
                     ELVGRSSLDFFHPEDRRAYQERGPSVAYERGIYVATYRYRHKDGHYVYLESTSRTLRD
                     PVSGELLEIISVSREVSRRVLAERATKRLARIVEATTDLVAFFEPNGRVTYLNEAARR
                     VLGIPYPENKCLKLAELMPAAAYAQIEREALPTARLGGVWSGETAFVDAMGREIPVSQ
                     VVLAHAGEHDEIDYFSTIARDISERRQAEEREQRHQAEMTHVARLITMGEMASGLAHE
                     LNQPLAAIVNYARGAIRRIHGEPPAELPVLAGVLERIAEQASRAGEIIKRLRSFARKS
                     EFRRAPVDLNKVVGDMIRFCRPEARRHALELRAELTGTLPAVLADRVQIEQVLLNLLR
                     NAMEATVQAQGSGHGPSTHDASLRTVTLS"
     gene            1919..2236
                     /locus_tag="PLQ18_03450"
     CDS             1919..2236
                     /locus_tag="PLQ18_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HNE00738.1"
                     /translation="MSLTPSPSERIMACPRFARLVAARSRFAWTLTAVVLGIYYGYMM
                     VVSFAPQLLAAPLSDGGTLPVGVPVGAAIIVLSWVLTGVYIRRANSEFDALTEQVLEE
                     HAP"
     gene            2290..3912
                     /locus_tag="PLQ18_03455"
     CDS             2290..3912
                     /locus_tag="PLQ18_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003448686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006847 - plasma membrane acetate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation acetate symporter"
                     /protein_id="HNE00739.1"
                     /translation="MLPLALLAAPAGPVEVQKQPVNVTAIVMFMIFVAMTMGITYWAA
                     SRTRSAKDFYTAGGGITGFQNGLAIAGDYMSAATLLGLSGLVFARGHDGFIYAIGFFV
                     GWPVIMFLMAERLRNLGKYTFADIVSYRLEQTPIRTLAASGTLAVVAFYLIVQMVGAG
                     KLIQLLFGLEYWMAVVLVGVIMVVYVTFGGMLATTWVQIIKACLLLGAGTFMFVLAMS
                     QFGFSFETLAQKAVAAHKSGIKLMAPGALLADPVSSVSLSLGLVFGLAGLPHIMMRFF
                     TVTNAREARKSVFYANGFIGYFFLIVCAMGLTAIVIVGTNPEYLDAAGKLKGGENMAA
                     MHLAKAVGGDLFLGFISAVAFATILAVVAGLALAGASAVSHDLYASVFKRGQADEAAE
                     MRVSKIATVVIGIVAIVLGILFENQNVAFMTALTFGVAASANFPVLILSMYWKGLTTR
                     GALIGGFTGLLSAVGFVVMGPTVWVKVLGNAAPLFPYDNPALFSMPLAFATIIIISKL
                     DGSARAARERAAYAAQFVRAQTGLGAEAAAEH"
     gene            4252..>4920
                     /locus_tag="PLQ18_03460"
     CDS             4252..>4920
                     /locus_tag="PLQ18_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019621643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNE00740.1"
                     /translation="MHHNPYELGLDQNPANFTALSPLSFIERTASIYPERTATVHGDV
                     RRTWGETFARCRRLASALKKRGIGAGDTVAVMLPNIPEMLEVHFGVPASGAVLNTLNV
                     RLDAEAIAFMLQHGEAKVLITDREFTDTIRKALHMLQTRRPLVIDVDDLQYSGGALLG
                     TLTYEDLLAEGDEDAPWVLPTDEWSAITLNYTSGTTGNPKGVVYHHRGAYLNAMCNIV
                     AWDMG"
BASE COUNT          731 a         1687 c         1680 g          822 t
ORIGIN      
        1 cgacagcgtc accgtgcgca gcgacgcatc gtgcgtgctc gggccgtggc cgctgccctg
       61 ggcctgcacg gtggcttcca tggcgttgcg cagcaggttc agcagcacct gctcgatctg
      121 tacgcgatcg gcgagcaccg ccggcagcgt tccggtgagt tcggcgcgca gttcgagcgc
      181 gtggcggcgc gcctcgggcc ggcagaagcg gatcatgtcg ccgacgacct tgttcagatc
      241 gaccggggcg cgccggaact cgctcttgcg cgcgaagctc ctgagccgct tgatgatctc
      301 gccggcccgg ctcgcctgct cggcgatgcg ctccagcacg ccggcgagca ccggcagctc
      361 ggccggcggc tcgccgtgga tgcggcggat cgcgccgcgc gcgtagttga cgatcgccgc
      421 cagcggctgg ttcagctcat gcgcgaggcc cgaggccatc tcgcccatcg tgatcaggcg
      481 cgcgacgtgc gtcatctcgg cctgatggcg ctgctcgcgc tcctcggcct gacgccgttc
      541 gctgatgtcg cgggcgatgg tcgagaagta gtcgatctcg tcgtgctcgc cggcgtgcgc
      601 gagcacgacc tgcgagaccg ggatctcgcg ccccatcgca tcgacgaagg ccgtctcgcc
      661 gctccagaca ccgccgaggc gtgccgtcgg cagcgcctcg cgctcgatct gcgcgtaggc
      721 ggcggccggc atcagctcgg cgagcttcag gcacttgttc tcgggatacg gaatgccgag
      781 cacgcgccgg gcggcctcgt tgaggtaggt gacgcggccg ttcggctcga agaaggcgac
      841 gagatcggtg gtcgcctcga cgatgcgcgc cagacgcttg gtggcgcgct cggcgagcac
      901 gcgccggctg acctcgcgcg ataccgagat gatctccagc agctcgccgc tgaccggatc
      961 gcgcagcgtg cggctggtgc tctccaggta cacgtagtga ccgtccttgt gccggtagcg
     1021 gtaggtcgcg acgtagatgc cgcgctcgta ggcgacgctc gggccgcgct cctgataggc
     1081 gcgccggtcc tcggggtgga agaaatccag cgaggacctg ccgacgagtt cctcgggcgc
     1141 gtagccgagc agcgtctgcg cggccggcga ggcgtacagg aaggtccagg tgcccggcga
     1201 gtggcgcgag atgatgtcgg tcgactgctc ggcgagcagg cggtggcgtg cctcgctgat
     1261 gcgcagcgcc gcctcggtgc ggcggctgtc ggtcacgtcg cgcccgagca cgtgcacggc
     1321 cgtgccgccg ccggagggca cgtaggtcca gcacagcgtg aacgggccga cgcgggtgtc
     1381 ggcgagttcg gcgatctcgt cgtggtacag gcattcgtgc acgtactcga cgtggcgtgc
     1441 cggcagcagc tgctccggtg catcgagccc gaccgtcccg gcgaggccgg cagcggccgg
     1501 gttgtacgcg agcacgctgc cgtcactgtt gcaggcgaac accgggtccg gcaggtgctc
     1561 ggcgagccgt gcaaacggcg ccgccgggcc gggggggcga agcgcagtca tcaggtcttg
     1621 ctccacgctc gaagcgatat aggtgaaaac cctagtatgc ctagctagtg tccgaaggtt
     1681 gttttcagta ttcttccgaa aatacctaga catcttcgca tgcaggagat gcacgcatga
     1741 cgggcggacg gttcacgaga ccgttcgtga ccggcatgac gaagcgcgga ccgcgcgtcg
     1801 cccaccggcg cgcacggagc gcgcacaacg acagaaaaca caccacaagc acacaacccc
     1861 agaacgactg gagcagcggt ccggctgcgg gccgcaaaga cctggagagc atcgaaacat
     1921 gtccctgacc ccgtctccgt ccgaacggat catggcctgt ccgcgctttg cgcggctggt
     1981 cgcggcccgt tcgcgctttg cctggacgct gaccgccgtc gtgctcggca tctattacgg
     2041 ctacatgatg gtcgtgtcct tcgcgccgca gctgctggcc gcaccgctga gcgacggcgg
     2101 cacgctgccg gtcggcgtgc cggtcggtgc cgcgatcatc gtgctgtcct gggtgctgac
     2161 cggcgtctac atccgccgcg ccaacagcga gttcgacgcg ctgaccgagc aggtgctcga
     2221 ggagcacgcg ccatgatcca cagtggcaac cgcttcattc cgcgcctgtt cctgctcgcc
     2281 ctcggcctga tgctgccgct ggccctgctc gccgccccgg ccggtccggt cgaagtgcag
     2341 aagcagccgg tcaacgtcac cgcgatcgtg atgttcatga tcttcgtcgc gatgacgatg
     2401 ggcatcacct actgggccgc ctcgcgcacg cgctcggcca aggacttcta caccgccggc
     2461 ggcggcatca ccggcttcca gaacggtctg gcgatcgccg gcgactacat gtcggcggca
     2521 acgctgctcg ggctgtcggg cctggtgttc gcacgcggcc atgacggctt catctacgcg
     2581 atcggcttct tcgtcggctg gccggtgatc atgttcctga tggccgagcg cctgcggaac
     2641 ctcggcaagt acacctttgc cgacatcgtc tcgtaccggc tcgaacagac gccgatccgc
     2701 acgctggccg cctccggcac gctggcggtg gtggccttct acctgatcgt gcagatggtc
     2761 ggtgccggca agctgatcca gctgctgttc gggctcgaat actggatggc cgtcgtgctg
     2821 gtcggcgtga tcatggtcgt ctacgtgacc ttcggcggca tgctcgcgac cacctgggtg
     2881 cagatcatca aggcctgcct gctgctcggg gccggcacct tcatgttcgt gctggcgatg
     2941 tcgcagttcg gcttcagctt cgagacgctg gcacagaagg ccgtcgccgc gcacaagtcc
     3001 ggcatcaagc tgatggcccc cggtgcgctg ctcgccgatc cggtgtcctc ggtgtcgctg
     3061 tcgctcggcc tggtgttcgg cctcgccggc ctgccgcaca tcatgatgcg cttcttcacg
     3121 gtcacgaacg cccgcgaggc gcgcaagtcc gtgttctacg ccaacggctt catcggctac
     3181 ttcttcctga tcgtctgcgc gatgggcctg accgccatcg tcatcgtcgg caccaatccc
     3241 gagtacctgg acgcggccgg caagctcaag ggcggcgaga acatggcggc gatgcatctg
     3301 gccaaggccg tcggtggcga cctgttcctc ggcttcatct cggccgtggc cttcgcgacc
     3361 atcctcgccg tggtcgccgg cctcgcgctc gccggtgcct cggccgtgtc gcacgacctg
     3421 tacgcgagcg tgttcaagcg cggccaggcc gacgaggccg cggagatgcg cgtctcgaag
     3481 atcgccaccg tcgtcatcgg catcgttgcg atcgtgctcg gcatcctgtt cgagaaccag
     3541 aacgtcgcgt tcatgaccgc gctgaccttc ggcgtcgcgg cctcggccaa tttcccggtg
     3601 ctgatcctgt cgatgtactg gaaggggctg accacgcgcg gcgcgctgat cggcggcttc
     3661 accggcttgc tcagcgccgt cggcttcgtg gtcatggggc cgaccgtgtg ggtcaaggtg
     3721 ctcggcaacg ccgcgccgct gttcccgtac gacaacccgg cgctgttctc gatgccgctg
     3781 gccttcgcca ccatcatcat catctcgaag ctcgacggca gcgcccgcgc cgcacgcgag
     3841 cgcgccgcct atgccgcgca gttcgtgcgt gcgcagaccg gtctcggtgc cgaggcggcc
     3901 gccgagcact gagccagagc ggctgctacg gtaggcgacc ggtcaccgtg cacaaggcgg
     3961 gtggccggtc gatgaccacg gtcggtcacg cagcggcacc ggatgcaggc cagccgccgg
     4021 ggggaggctc gcatgattcg tccggttgcc gctcgcctgc cagggcagcg gcaggggctg
     4081 cgccggcctg cccgcgcagg ccggcccgca gaagaaagca agcacgcgca ggctttgatg
     4141 cctgccccga cccgcgccaa ccccggtggc agggccgctg atcgcggcag cgccaccgga
     4201 ttcaccgggc aagcgcaacg acccgtcccg ggtcagcagg agtgaggaac gatgcaccac
     4261 aacccgtatg aactggggct cgaccagaac cccgcgaact tcacggcgct gtcgccgctg
     4321 agcttcatcg agcgcacggc gtcgatctat ccggagcgca cggcaaccgt gcacggcgac
     4381 gtgcgccgca cctggggcga gaccttcgcg cgctgccggc gcctcgcctc ggcgctgaag
     4441 aagcgcggca tcggcgccgg cgacacggtc gcggtgatgc tgccgaacat tcccgaaatg
     4501 ctcgaagtgc acttcggcgt gccggcctcg ggggcggtgc tgaacacgct gaacgtgcgt
     4561 ctcgacgccg aggccatcgc cttcatgctg cagcatggcg aggcgaaggt gctgatcacc
     4621 gaccgcgagt tcaccgacac gatccgcaag gccctgcaca tgctgcagac gcgccggccg
     4681 ctggtcatcg atgtcgatga cctgcagtac agcggcggcg cgctgctcgg cacgctgacc
     4741 tacgaggatc tgctcgccga aggcgacgag gatgcgccgt gggtgctgcc gaccgacgaa
     4801 tggtcggcga tcacgctcaa ctacacctcc ggcaccaccg gcaatccgaa gggcgtggtc
     4861 tatcaccacc gcggtgctta tctgaacgcg atgtgcaaca tcgtcgcctg ggacatgggc
//
LOCUS       DAOQJH010000098         4915 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_10146_length_4915_cov_4.022016, whole genome shotgun sequence.
ACCESSION   DAOQJH010000098 DAOQJH010000000
VERSION     DAOQJH010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 4915)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4915)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4915
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10146_length_4915_cov_4.022016"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3444)
                     /gene="uvrA"
                     /locus_tag="PLQ18_03465"
     CDS             complement(<1..3444)
                     /gene="uvrA"
                     /locus_tag="PLQ18_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011661121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HNE00741.1"
                     /translation="MTDPRTPVVPATGAIRLRDAHQNNLKHLDLDFPTGRLTVVTGVS
                     GSGKSSLVFDTLYAEGQRRYVETFSPYARQFLDRQDRPQVGSVAGIPPAIAIDQTNPV
                     RTSRSTVGTMTELNDHLKLLYARMSTPCCRGCGRPVRRDGPDSILDDLHARAAAAGDP
                     RLAVSFPVAVRDQAGADVEALLAQQGYTRIQAREPGWLHVVQDRFRLGTAERARVIEA
                     LETALRQGRGRVDVHVLDPAPAPDGDSPEAPAPRWRYSADLHCPDCDIHYAEPVPAAF
                     SFNSPLGACPICRGFGRVIDVDFGLVVPDASKTLAQGAVKPWQTESFRECQQDLEKYA
                     RLRGVALDVPFRDLPPEHRHWVLEGEDGWQSWERSWPKLWYGVRHFFDWLESKAYKMH
                     IRVLLSKYRAYTPCTACAGARLKPESLDFRLGTRAEADAVLAPARRFLPTQVRYRRAV
                     LETLPGLTLHDLMLLPLTRLASFFAQLRLPAPLDEATGLVLGEIRTRLRFLGEVGLGY
                     LTLDRQSRTLSGGEVQRINLTTALGTSLVNTLFVLDEPSIGLHPRDMHRVIAVMQRLR
                     AQGNTLVVVEHDPQIMFAADRLLDLGPGPGARGGEVMFFDAPARLAGARGSLTADYLL
                     GRRHVAAGRVPRPVDDNTPRLRLCGAAEHNLKALDVAIPLGRLVCVTGVSGSGKSTLV
                     QEVLVPALARRLGRPTEAPGAFRALEGGLEIGEVVVVDQSPIGKTARSNPASYIGAFD
                     AIRKLFAGTDAARERGYTPGTFSFNAGNGRCPGCQGSGFEHVEMQFLSDVYLRCPDCD
                     GTRFRAEVREVELAGKSIADVLDLSVAEAIDFFGPLRGGREVVRLLAPLAEVGLDYLR
                     LGQPVPTLSGGEAQRLKLAGHLVESAGRQRRRRGDEPEPQTLFVFDEPTTGLHFDDIA
                     RLLQAFGRLLDAGHTLLVIEHNLDVIDAADWLLDLGPEGGDDGGELLCAGPPDAVIAC
                     AASHTGRALAQYRADMQRLAEGRADAVAEAPASYAAAPPDAIRIHHAREHNLKNVSVE
                     IAHRGFTVITGPSGSGKSTLAFDIVFGEGQRRYLESLNAYARQFVQPAARPEVDAVHG
                     IPPTVAIEQRVSRGGRKSTVATLTETYHFLRLLWMKLGTQYCPDCARPIEPQSEDAIA
                     AK"
     gene            complement(3453..3869)
                     /locus_tag="PLQ18_03470"
     CDS             complement(3453..3869)
                     /locus_tag="PLQ18_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009625644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HNE00742.1"
                     /translation="MIIADTGFWIALAMPDDRFHERACRQLADLKERLIVTWPVITET
                     CHLLGKRVSRHAQQLFLQHLATGVLDIFDLRDIHLPRVIELMQRYANLPMDLADASLV
                     VLAEHLGDGRILSTDMRDFGTYRWKNTAPFRNLLAD"
     gene            complement(3856..4119)
                     /locus_tag="PLQ18_03475"
     CDS             complement(3856..4119)
                     /locus_tag="PLQ18_03475"
                     /inference="COORDINATES: protein motif:HMM:NF013561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HNE00743.1"
                     /translation="MRIQSRLDEAHARKLDEIRRMTGASISHVIEQALDLYHDRLVTG
                     QPSTYQALLDSGFIGCGPQGQTELSTEYKKAFAAIVEDGHDHR"
     gene            4440..>4915
                     /locus_tag="PLQ18_03480"
     CDS             4440..>4915
                     /locus_tag="PLQ18_03480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE00744.1"
                     /translation="MSHVLSRIPSFNALRGLCALLLLAASVFAHAFGLGLGGPSDTEP
                     AARAGWWYAAPGVETRFELQAGETRMLPLGELVVGAFDSARVLVPGVPADEDRYRLEF
                     ELQPEGSAPVHISASAVVGRAGDAHGERLTFDFGAEPVQIGFGRGGVLSVHFRPLDL"
BASE COUNT          710 a         1648 c         1805 g          752 t
ORIGIN      
        1 cttcgcggcg atcgcatcct cgctctgcgg ctcgatcggg cgcgcgcagt ccggacagta
       61 ctgcgtgccg agcttcatcc acagcaggcg caggaagtga taggtctcgg tcagcgtcgc
      121 caccgtcgac ttgcgcccgc cgcgcgacac gcgctgttcg atcgcgaccg tcggcgggat
      181 gccgtgcacg gcatcgacct ccgggcgcgc cgccggctgc acgaactggc gcgcgtaggc
      241 gttcagcgat tcgagatagc gccgctggcc ctcgccgaac acgatgtcga aggcgagcgt
      301 cgacttgccg ctgccggacg ggccggtgat caccgtgaag ccgcggtgcg cgatctcgac
      361 gctgacgttc ttcaggttgt gctcgcgggc gtgatggatg cggatggcgt cgggcggggc
      421 ggccgcgtag ctcgccggtg cctcggcgac ggcgtcggcg cggccttcgg cgagccgctg
      481 catgtcggcg cggtactgcg ccagcgcccg tccggtatgc gaggcggcgc aggcgatcac
      541 ggcatccggc gggccggcgc acagcagctc gccgccgtcg tcgccgcctt cggggccgag
      601 gtcgagcagc cagtcggcgg cgtcgatgac atcgaggttg tgctcgatga ccagcagcgt
      661 gtggccggca tcgagcaggc gtccgaaggc ctggaggagc cgggcgatgt cgtcgaagtg
      721 caggccggtg gtcggttcgt cgaacacgaa cagcgtctgc ggctcgggct cgtcgccgcg
      781 gcgccggcgc tgccggccgg cggattcgac gaggtggccg gcgagcttca ggcgctgcgc
      841 ctcgccgccc gagagcgtcg gcaccggctg gccgaggcgc aggtaatcga gcccgacctc
      901 ggcgagcggt gcaagcaggc gcacgacctc gcggccgccg cgcagcgggc cgaagaagtc
      961 gatggcctcg gcgacgctca ggtcgagcac gtcggcgatg gattttccgg ccagttcgac
     1021 ctcgcgcacc tcggcgcgaa agcgcgtgcc gtcgcagtcc gggcagcgca gatagacatc
     1081 ggacaggaac tgcatctcga cgtgctcgaa gcccgagccc tgacagcccg ggcagcggcc
     1141 gttgccggcg ttgaagctga aggtgccggg cgtgtagccg cgctcgcgcg ccgcgtcggt
     1201 gccggcgaac agcttgcgga tcgcgtcgaa ggcgccgatg tagctcgccg ggttcgagcg
     1261 cgcggtcttg ccgatcggtg actggtcgac gacgacgacc tcgccgatct ccagcccgcc
     1321 ttcgagcgcc cggaacgcgc cgggcgcctc ggtcggcctg ccgagccggc gggccagcgc
     1381 cggcaccagg acttcctgca ccagcgtcga cttgccggag ccggagacgc cggtcacgca
     1441 caccagccgt ccgagcggga tcgcgacatc gagggccttc aggttgtgct cggccgcgcc
     1501 gcacagccgc agccgcggcg tgttgtcatc gaccggccgc ggcacgcgcc cggcggcgac
     1561 gtggcggcgg ccgagcaggt agtcggcggt cagtgaaccg cgcgctccgg ccagccgcgc
     1621 cggggcatcg aagaacatga cctcgccgcc gcgtgcgccg gggccggggc cgaggtcgag
     1681 cagccggtcg gccgcaaaca tgatctgcgg atcgtgctcg accaccacca gcgtgttgcc
     1741 ctgcgcgcgc aggcgctgca tcaccgcgat cacgcggtgc atgtcgcgcg ggtgcaggcc
     1801 gatgctcggc tcatcgagca cgaacagcgt gttgaccagc gaggtgccga gcgccgtggt
     1861 caggttgatg cgctgcacct cgccgccgga gagcgtgcgc gactggcgat cgagcgtcag
     1921 gtagccgagc ccgacctcgc cgaggaagcg caggcgcgtg cggatctcgc cgagcaccag
     1981 tccggtggcc tcgtcgagcg gggccggcag ccgcagctgc gcaaagaagc tcgcgagccg
     2041 cgtcagcggc agcagcatca ggtcgtgcag cgtcaggccg ggcagggttt cgagcacggc
     2101 gcgccggtag cgcacctgcg tcggcaggaa gcgccgcgcc ggtgccagca ccgcgtcggc
     2161 ttcggcgcgc gtgccgagac ggaaatccag cgattcgggc ttcagccgcg cgccggcgca
     2221 ggccgtgcag ggcgtgtagg cgcggtactt tgagagcagc acacggatgt gcatcttgta
     2281 cgccttgctc tccagccagt cgaagaagtg gcgcacgccg taccagagct ttggccacga
     2341 gcgctcccag ctctgccagc cgtcctcgcc ttcgagcacc cagtgccggt gctcgggcgg
     2401 caggtcgcga aacggcacgt cgagcgcgac cccgcgcagc cgcgcgtact tttcgagatc
     2461 ctgctggcac tcgcggaagc tctcggtctg ccacggcttc acggcgccct gcgcgagcgt
     2521 cttcgatgcg tcgggcacga ccagcccgaa atcgacatcg atgacccgcc cgaagccgcg
     2581 gcagatcggg caggccccga gcggcgagtt gaacgagaac gccgccggca ccggctcggc
     2641 gtaatggatg tcgcagtccg ggcagtgcag gtcggccgaa tagcgccagc gcggggcggg
     2701 cgcttcgggc gagtcgccgt ccggagcggg tgcagggtcg agcacatgca cgtcgacccg
     2761 cccgcgcccc tgacgcagcg cggtttccag cgcctcgatc acgcgcgccc gttcggccgt
     2821 gccgagccgg aagcggtcct gcacgacgtg cagccagccg ggttcgcggg cctggatgcg
     2881 cgtatagccc tgctgcgcga gcagcgcctc gacatcggcc ccggcctgat cgcggacggc
     2941 gaccgggaag ctcacggcca gacgcggatc gccggccgcg gcggcgcggg cgtgcaggtc
     3001 atcgaggatg ctgtccggcc cgtcgcggcg caccggccgg ccgcagccgc ggcagcacgg
     3061 cgtgctcatg cgcgcgtaca gcagcttcag atggtcgttc agctcggtca tcgtgccgac
     3121 cgtcgagcgc gaggtgcgca ccggattggt ctggtcgatc gcgatcgccg gcggaatccc
     3181 cgccaccgag ccgacctgcg gccggtcctg ccggtcgagg aactggcgcg cgtaggggct
     3241 gaaggtctcg acgtagcgac gctggccttc ggcgtacagc gtgtcgaaca ccagcgagga
     3301 cttgcccgaa cccgagacgc cggtgacgac ggtcagccgg ccggtcggga agtcgaggtc
     3361 gaggtgcttg aggttgttct ggtgcgcatc ccgcagccgg atcgcgccgg tggcgggcac
     3421 gacgggcgtg cggggatcgg tcatcggggg gatcagtcgg ccagcaggtt gcggaacggg
     3481 gcggtgttct tccagcgata ggtgccgaaa tcgcgcatgt cggtggacag gatgcggccg
     3541 tcgcccagat gttccgcgag cacgaccagg gaggcgtcgg ccaggtccat cggcaggttg
     3601 gcgtagcgct gcatgagttc gatgacacgc ggcaaatgga tgtcgcgcag gtcgaagata
     3661 tcgaggactc cggtggcgag gtgctggagg aacaactgct gcgcgtgccg gctgacgcgc
     3721 ttgcccagca gatggcaggt ttccgtgatc accggccagg tcacgatcag ccgctccttc
     3781 aggtccgcga gctggcggca cgcccgttca tgaaaacggt cgtccggcat ggccagcgcg
     3841 atccagaaac cggtgtcagc gatgatcatg tccgtcttcg acgatggcgg cgaatgcctt
     3901 cttgtattcc gtggaaagct ccgtctggcc ttgcgggccg cagccgatga agcctgagtc
     3961 cagcagggcc tgatacgtcg acggctgccc ggtcacgagc cggtcgtgat acaggtcgag
     4021 cgcctgctcg attacgtggc tgatgctggc gccggtcatt cgccggattt cgtcgagctt
     4081 gcgggcgtgc gcttcatcga gtctggactg gatgcgcatg gcatgactcc gggggcaggc
     4141 gcgaatggac ggccagccta ggcccggccg cttgcgtccg gcaatgccgg ttccggtatc
     4201 ccggccatgg cctgccggca ggcgtgaatg tgtccggttg cgtcaggcga ttttacaaac
     4261 catgtcgggt tcaggatgcg gtggttgaga taattgcttt catggcaatg acttgattaa
     4321 ttggctcggg tattgcttag attcactgtg tctgtccaaa agttcactgg accctgcccg
     4381 gcgtcattga ggaacccgga tcgttcacgc cgttcccccc accgatcccg gcccgaacca
     4441 tgtcgcacgt cctgtcccga atcccgagct tcaatgccct gcgcggcctg tgcgcgctgc
     4501 tgctgctggc ggcgagcgtg ttcgcccatg ccttcgggct tggtctgggc ggcccgtccg
     4561 acaccgaacc ggctgcccgc gccggctggt ggtatgccgc accgggggtc gagacccgct
     4621 tcgagctgca ggccggcgag acgcgcatgc tgccgctcgg cgagctggtc gtcggggcct
     4681 tcgacagcgc ccgcgtgctg gtgccgggcg tgccggccga cgaggaccgc taccggctcg
     4741 aattcgagct gcagcccgag ggcagtgcac cggtgcacat cagcgccagc gccgtggtcg
     4801 ggcgcgccgg cgatgcgcac ggcgagcggc tgaccttcga cttcggcgcc gagccggtgc
     4861 agatcggctt cggccgcggc ggcgtgctca gcgtgcattt ccggccgctt gacct
//
LOCUS       DAOQJH010000099         4894 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_10203_length_4894_cov_3.158091, whole genome shotgun sequence.
ACCESSION   DAOQJH010000099 DAOQJH010000000
VERSION     DAOQJH010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 4894)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4894)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4894
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10203_length_4894_cov_3.158091"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..162
                     /locus_tag="PLQ18_03485"
     CDS             1..162
                     /locus_tag="PLQ18_03485"
                     /inference="COORDINATES: protein motif:HMM:NF015424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HNE00745.1"
                     /translation="MKTGAVSSEVTLTTTGGLSIAATTTQEATSELGLVVGAPAFALV
                     KASAVIVGV"
     gene            complement(256..735)
                     /locus_tag="PLQ18_03490"
     CDS             complement(256..735)
                     /locus_tag="PLQ18_03490"
                     /inference="COORDINATES: protein motif:HMM:TIGR00638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HNE00746.1"
                     /translation="MYAIQTQGEFRHTEELSIGNRLPGRIVEIVRGPVMSEIELDTQA
                     GMVTAAITTRSLQRMRLAEGQDVLALIKATELAIEKPAAAEALLASSFRNQLEARVVA
                     LEAGTVLTAVELDTVVGPLTAVITARSARRLKLAPGDQVIGVVKATEVAIAREGRRR"
     gene            complement(750..1277)
                     /locus_tag="PLQ18_03495"
     CDS             complement(750..1277)
                     /locus_tag="PLQ18_03495"
                     /inference="COORDINATES: protein motif:HMM:NF012401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNE00747.1"
                     /translation="MTSVMPAAADVRVPALRLDGRVLRPLRLDAGALQGLVQHTVVRR
                     ALSCAARSLDPAVGLRCWQGVRLTALLELAGLQLDRQRERGGVCVHAHGRDGYVASFS
                     WSELFNGPAGGDVLVALGVDGMPLPAGDSPRLIAAHDWQSSARLVRGLCRIEVAGPVA
                     LRPDPGTAMTMTSRR"
     gene            1514..2332
                     /locus_tag="PLQ18_03500"
     CDS             1514..2332
                     /locus_tag="PLQ18_03500"
                     /inference="COORDINATES: protein motif:HMM:TIGR00638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HNE00748.1"
                     /translation="MSRSPADSMQLEGRFWLDRGGKPFLGPGRVALLERIDACRSIAE
                     AARAMGMGYKTAWDLVDAMNQLAEQPLVVRVKGGRQGGGTELTDYGRSVVATFRALER
                     DYVAMLETLGARYPGFGAFDAHPRPPRLRTSARNQWSGHVTGIERHGFGALVTVDIGA
                     GAPVQAALSMNAVEELGLTDGSEVHVLVKATAIGIGVVAGWGGSGGNRLHGVIEHLQL
                     DAGGERAELTLAIAASRHVLAIIDPAMIAALGLQAGVAAWAQFEPAAVILAVGD"
     gene            2426..3547
                     /gene="thiO"
                     /locus_tag="PLQ18_03505"
     CDS             2426..3547
                     /gene="thiO"
                     /locus_tag="PLQ18_03505"
                     /EC_number="1.4.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005959909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016647 - oxidoreductase activity, acting
                     on the CH-NH group of donors, oxygen as acceptor [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine oxidase ThiO"
                     /protein_id="HNE00749.1"
                     /translation="MSTACTLPVTQCDCIVIGAGASGLLVARLLARAGLKLVVLERGA
                     IGRESSWAGGGILSPLYPWRYDPAVNALAGWSQRAFAALAAGLHAQTGIDPEWTPNGM
                     LYLGGLETEAARAWAVAQAASLEALTPPDARGIEPRLARQQQCALWMAQIAQVRNPRL
                     MQALAREAQQLGIDVREHVEVHGPVIRGGRVAGVRTAAGRFEAPRVVVATGAWTGALL
                     AGLPQPPAVAPVRGQMIVFRAAPGWLTRIVMRDAHYLIPRRDGRILAGSTVEFVGFDK
                     STTDEAFKTLHAAALAIAPGLADIPVEHHWAGLRPGAPAGIPYIGEHPGIGGLYVNAG
                     HYRNGLVTGPASAQLLANLILGETPILDPAPYGFGAGRL"
     gene            3704..>4894
                     /locus_tag="PLQ18_03510"
     CDS             3704..>4894
                     /locus_tag="PLQ18_03510"
                     /inference="COORDINATES: protein motif:HMM:NF017727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HNE00750.1"
                     /translation="MNPAAAPAAPPDRLAVLTAAVHRPMFLAGAVQLLATVLFWLGEL
                     WLRRSGQTLATVLPATWAHVLLMLYGSFTFFIFGFLMTVYPRWMDGPELVRAQFLPTV
                     GWLAGGQALVWIGLFSSKTVLGLGIAAMLAGWGLGAARLYGVHFDTGRGKLQERVLNG
                     ALAGGWLGLASFLLWLLSGDAVFLMFALKAGLWFWLLPVFVTVCHRMIPFFGSCVLPG
                     YAIVRPDALLKLWAGGSALHGLLELANLPTWTWIIDLPMAASAAWIAWQWQPARALKI
                     RLLGVLHIAFWWFGFAMFLYGLNALLLAFGGNGLGRAPLHAIGIGLCAALVIAMATRV
                     TLGHSGGRLMADDYTWYCFLAINAAALARVAADVHGWPTLNLVAAALWIAAFGAWVWK
                     YAPIY"
BASE COUNT          619 a         1854 c         1658 g          763 t
ORIGIN      
        1 gtgaagaccg gcgcggtcag cagcgaggtg acgctgacca cgaccggtgg cctgagcatc
       61 gccgcgacca ccacgcagga agccacgtcc gagctcggcc tcgtggtcgg cgcgccggcg
      121 tttgcgctgg tcaaggccag cgcggtgatc gtcggcgtct gatccaccgc ctgccggcct
      181 gtttccggac ctgcgccgcc gcgccggcgc aggtccggcg cagcagggcc tgcgaagcga
      241 ctgcgaagcc ggcgactagc gccgccggcc ctcgcgggcg atcgccacct cggtcgcctt
      301 gaccacgccg atcacctgat cccccggggc gagcttcagc cgccgggcgc tgcgcgccgt
      361 gatcaccgcg gtcagcggac cgacgacggt gtcgagttcg accgccgtca gcacggtgcc
      421 ggcctcgagc gcgacgacgc gcgcttcgag ctggttgcgg aagctgctcg cgagcagcgc
      481 ctcggccgcc gcgggcttct cgatcgccag ctccgtggcc ttgatcagcg ccagcacgtc
      541 ctggccctcg gccagacgca tgcgctgcag gctgcgggtc gtgatcgccg ccgtgaccat
      601 gccggcctgg gtgtcgagct cgatctccga catcaccggg ccgcgcacga tctcgacgat
      661 gcgcccgggc aggcggttgc cgatgctgag ttcctcggtg tgccggaact ctccctgtgt
      721 ctgaatcgcg tacatgtccc ctgctcccct catcttctgc ttgtcatggt catcgcggtg
      781 cccggatccg gtcgcagcgc gaccggtccg gcaacctcga tgcggcagag tccgcgcacc
      841 agccgggcgc tgctctgcca gtcgtgtgcg gcaatgagcc gcggtgaatc cccggccggc
      901 agcggcatgc cgtcgacgcc gagcgccacc agcacgtccc cgccggccgg gccgttgaac
      961 agttcgctcc aggaaaagct cgcgacgtag ccatcccgcc cgtgcgcatg cacgcagacc
     1021 ccgccgcgct cgcgctggcg atcgagctgc aggccggcga gttcgagcag tgccgtcagc
     1081 cgcacgccct gccagcagcg caggccgacg gccgggtcga ggctgcgggc tgcgcagctc
     1141 aatgcccggc gcactaccgt atgctgcacc agcccctgca aggccccggc atcgaggcgc
     1201 agcggtcgca gcacccggcc gtcgagccgc agtgcgggta cgcggacatc cgctgctgcc
     1261 ggcatcaccg acgtcatgaa aggcctcccg gtccggtttt ctatatccgt accaatacaa
     1321 taaatctgcc atcgctcatg ctgcatcgca tcaggctgaa accgttgact ttccccgtca
     1381 caggcccggc gccggccctg ccgttgcggc cgccggcagc gatgtcgcga ctgcgaccgc
     1441 ccgggtcgcg tttgtcggct ttgcgacgcg aaaaccccgg ttcgcatgcg gtttgcagcg
     1501 cgcggagccg gccgtgagcc gcagccctgc tgactcaatg caactcgaag gccgtttctg
     1561 gctcgatcgc ggtggcaaac cgtttctcgg gcccgggcgc gttgcgctgc tggagcgcat
     1621 cgatgcctgc cgctcgatcg ccgaagccgc gcgggcgatg ggcatgggtt acaagacggc
     1681 ctgggatctg gtcgatgcga tgaaccagct cgccgaacag ccgctggtcg tgcgcgtgaa
     1741 gggcggccgt cagggcggcg gcaccgagct gaccgattac ggccgcagcg tggtcgcgac
     1801 cttccgcgcg ctggagcgcg attacgtggc gatgctcgaa accctcggag cccgttaccc
     1861 cggcttcggt gccttcgacg cccatccgcg cccgccgcgc ctgcgcacca gcgcccgcaa
     1921 ccagtggagc ggccacgtca ccggcatcga gcgccacggc ttcggcgcgc tggtcacggt
     1981 cgacatcggt gccggcgcgc cggtgcaggc ggcgctcagc atgaacgccg tcgaggaact
     2041 cggcctcacc gacggcagcg aggtgcacgt gctggtcaag gcgaccgcga tcggcatcgg
     2101 cgtggtcgcc ggctggggcg gcagcggcgg caaccgcctg cacggcgtca tcgagcatct
     2161 gcagctcgat gccggcggcg agcgcgcgga gctgacgctc gcaatcgccg ccagccgcca
     2221 cgtgctcgcc atcatcgatc cggccatgat cgccgcgctc ggcctgcagg ccggcgttgc
     2281 cgcctgggcg cagttcgagc cggccgcggt gatcctcgcc gtcggcgact gaaccccggc
     2341 agcccttttc ccggacggcg atgggtgcaa agctgcgcgc cccgccgaat ccatccgccg
     2401 cgatccgccg cgaggagaat ccgccttgag cacggcctgc actctcccgg tcacgcagtg
     2461 cgactgcatc gtcatcggcg ccggcgccag cggcctgctg gtcgcccggc tgctggcgcg
     2521 cgccggcctg aaactcgtcg tgctggagcg cggcgccatc ggtcgcgaat cctcgtgggc
     2581 cggcggcggc atcctgtcgc cgctgtaccc gtggcgctac gacccggccg tcaacgcgct
     2641 cgccggctgg agccagcgcg cgttcgcagc cctcgccgcc ggcctgcatg cgcagaccgg
     2701 catcgacccg gaatggacgc cgaacggcat gctgtatctg ggcggcctcg aaaccgaagc
     2761 cgcgcgcgcc tgggccgtcg cgcaggccgc gagcctcgaa gccctgaccc cgcccgatgc
     2821 ccgcggcatc gagccacgcc tcgcccgcca gcagcagtgc gcgctgtgga tggcgcagat
     2881 cgcccaggtg cgcaacccgc ggctgatgca ggcgctcgcg cgcgaggcgc agcagctcgg
     2941 catcgacgtg cgcgagcacg tcgaggtgca cgggccggtg atccgcggcg ggcgcgtggc
     3001 cggcgtgcgc actgcggccg gacgcttcga ggcgccgcgc gtcgtggtcg ccaccggcgc
     3061 ctggaccggc gccctgctcg ccggcctgcc gcagccgccg gcggtcgccc cggtgcgcgg
     3121 gcagatgatc gtgttccgcg ccgcaccggg gtggctcacg cgcatcgtca tgcgcgatgc
     3181 gcactacctg atcccgcgcc gcgacggccg catcctcgcc ggctcgacgg tcgagttcgt
     3241 cggcttcgac aaatcgacca ccgacgaagc cttcaagacc ctgcacgccg ccgcgctcgc
     3301 catcgcgccg gggcttgccg acatcccggt cgagcaccac tgggccggcc tgcgccccgg
     3361 cgccccggcc ggcatcccgt acatcggcga gcaccccggc atcggcggcc tgtacgtcaa
     3421 cgccggtcac taccgcaacg gtctggtcac cggcccggcc agcgcgcagc tgctcgccaa
     3481 cctgatcctc ggcgaaaccc cgatcctcga tccggcgccc tacggcttcg gcgccgggcg
     3541 gctgtagccg gcccgacccc gcctccccgg caccggcccg gctgccggcc ggtgccgacc
     3601 gctcctgcgc cacggccccg tgcttgatgt tcatcaaggc accgccgcac tgcgccccct
     3661 gcaatgcggc ctccccctct gccgaatcgc cggaaccgcc gtcatgaacc ccgctgccgc
     3721 ccccgccgca ccgcccgatc gcctcgccgt cctcaccgcc gccgtgcacc gcccgatgtt
     3781 cctcgccgga gccgtgcaac tgctcgccac cgtgctgttc tggctcggcg aactctggct
     3841 gcgacgctcg ggccagacgc ttgcgaccgt gctgccggcg acctgggcgc acgtgctgct
     3901 gatgctctac ggcagcttca cgttcttcat cttcggcttc ctgatgaccg tctatccgcg
     3961 ctggatggat ggccccgaac tggtgcgcgc gcagttcctg ccgaccgtcg gctggctcgc
     4021 cggcgggcag gcgctggtgt ggatcggcct gttcagcagc aagaccgtgc tcgggcttgg
     4081 catcgccgcg atgctggccg gctggggcct cggtgcggcg cggctctacg gcgtccactt
     4141 cgataccggc cgcggcaagc tgcaggagcg cgtgctcaat ggcgcgctcg ccggcggctg
     4201 gctcgggctg gcgagcttcc tgctctggct gctgtcgggc gatgcagtct tcctgatgtt
     4261 cgcgctgaag gccggcctgt ggttctggct gctgccggtg ttcgtcaccg tctgccaccg
     4321 catgatcccg ttcttcggct cctgcgtgct gcccggctac gccatcgtgc gtcccgacgc
     4381 gctgctgaag ctctgggccg gcggctcggc cctgcacggc ctgctcgaac tggcaaacct
     4441 gccaacctgg acctggatca tcgacctgcc gatggccgcg agcgcggcgt ggatcgcctg
     4501 gcagtggcag ccggcgcgcg cgctgaagat ccgcctgctc ggcgtgctgc acatcgcctt
     4561 ctggtggttc ggcttcgcga tgtttttgta tggcctgaac gccttgctgc tcgccttcgg
     4621 cggcaacggt ctggggcggg cgccgctgca cgccatcggc atcggtcttt gtgcggcgct
     4681 ggtgatcgcg atggcgaccc gcgtcacgct cggccactcg ggcgggcggc tgatggccga
     4741 tgactacacc tggtactgct tcctcgccat caacgccgcc gccctggcgc gcgttgccgc
     4801 cgatgtgcac ggctggccga cgctgaacct cgtcgccgcc gccctctgga tcgccgcctt
     4861 cggtgcctgg gtgtggaaat acgcaccgat ctac
//
LOCUS       DAOQJH010000100         4875 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_10244_length_4875_cov_4.812033, whole genome shotgun sequence.
ACCESSION   DAOQJH010000100 DAOQJH010000000
VERSION     DAOQJH010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 4875)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4875)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4875
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10244_length_4875_cov_4.812033"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..993)
                     /locus_tag="PLQ18_03515"
     CDS             complement(<1..993)
                     /locus_tag="PLQ18_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNE00751.1"
                     /translation="MGTQAHAGSRHHLELVTIYEISKILSRSLDLRRSLHDVLNLLAT
                     HLHVRRAMVCLSAGDGHDLQVVAETGLSPDEAKRAQFRTGEGIIGRIFKSGMPIVVPD
                     INNEPLYLHRTAERPQPGEIVAFLGVPLREGTNVVGVLSVDRRTEGEPASRFDTDLRF
                     LMMIGNLIGQTIRLHRMVEHAQENLRVENRRLATELKGKYTIENVIGRSKRMQQVFAE
                     VHQAAPGRSTVLLRGESGTGKEVIARAIHTLSNRHDGPFVKVNCAALPESLLESELFG
                     HEKGAFTGATQERKGRFEMAHGGTLFLDEIGDISPGFQAKLLRVLQEREVSRLGS"
     gene            1307..1675
                     /locus_tag="PLQ18_03520"
     CDS             1307..1675
                     /locus_tag="PLQ18_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019865067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HNE00752.1"
                     /translation="MVMLTDKAVDAVRGVLTAQTETVAGLRVLVKTGGCAGYEYALGL
                     EVEPAADDAVLAYGDDVKVFVDPVSLPLLDGVTVDFVEDITGVGFRFDNPNAVKTCGC
                     GSSFSTDANAPGGTCSSKRA"
     gene            1681..2604
                     /gene="nifU"
                     /locus_tag="PLQ18_03525"
     CDS             1681..2604
                     /gene="nifU"
                     /locus_tag="PLQ18_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012973280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein NifU"
                     /protein_id="HNE00753.1"
                     /translation="MWDYSDKVKDYFFNPVNAGVLEEANAVGDVGSISCGDALRLMLK
                     VDPETDVILDARFQTFGCGSAIASSSALTEMIKGMTLDQALKVTNQDIAAYLDGLPPE
                     KMHCSVMGAEALHAAIANYRGEEWKDDHEEGELVCKCFGVDAALIERTVRDNGLVTVE
                     QVANYTKAGGGCCSCHEKIEDILIAVNGELAATAKPAIVQQPKPRTLVQIAPLKPEPA
                     PAPAKPAMTMVRKIQLIEKVLEDIRPSLKMDGGNAELIDVDGDTVYLHLTGACMGCSS
                     SGLTIAGIQERLMLAFGKPMRVVPSKPTAEV"
     gene            2609..3817
                     /gene="nifS"
                     /locus_tag="PLQ18_03530"
     CDS             2609..3817
                     /gene="nifS"
                     /locus_tag="PLQ18_03530"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019604922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HNE00754.1"
                     /translation="MNAPVYLDNNATTRVDPAVLAEMLPFFTELFGNPSSMHHYAAGV
                     AEAVQLARRQVQSLLGAKHDSEIIFTSCGTEANNTALYSALAANPGRREIITSVVEHP
                     AILAVCEHLEKNEGVTVHRIPVDAKGRLEIGAYQAALSDKVAIVSFMSANNETGTLFP
                     VEELAVMAKEVGALFHTDAVQAVGKLPMNLADSAIDMLSLSGHKLHAPKGVGALYLKR
                     GTRFRPLLRGGHQERGRRAGTENVTGIVGLGKAAELAQAAMEHENTVVRTLRDRLQTG
                     ILQSISHCFVTGDIEHRLPNTVNIAFEYIEGEAILLLLSRAGIAASSGSACTSGSLEP
                     SHVMRAMNIPYTAAHGSTRFSLSRDTTDADVDRVLAVMPEIIARLRQLSPYWGKDGPK
                     ATPDFAPAYA"
     gene            4029..4433
                     /locus_tag="PLQ18_03535"
     CDS             4029..4433
                     /locus_tag="PLQ18_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNE00755.1"
                     /translation="MTRQIFVNLPVRDLARTRSFFESLGFEFNEKFSNDEAACLIVGD
                     GRYVMLLAENFFSTFTHKPVCDARSATEVLVCLGCDSRAEVDDLVARARAAGGSVPRD
                     AQDHGFMYAHAFEDPDGHIWELVYMAENAEAA"
BASE COUNT          808 a         1674 c         1581 g          812 t
ORIGIN      
        1 cgagcccagg cgcgagacct cacgctcctg cagcacgcgc agcagcttgg cctgaaaccc
       61 cggcgagatg tcgccgatct cgtcgaggaa cagcgtgccg ccgtgggcca tctcgaagcg
      121 gcccttgcgc tcctgcgtgg cgccggtaaa ggcgcccttc tcgtggccga acagttcgga
      181 ttcgagcagc gattcgggca gggccgcgca gttgaccttc acgaacggcc cgtcgtggcg
      241 gttgctgagc gtgtggatgg cgcgggcgat gacttccttg ccggtgccgc tttcgccgcg
      301 cagcagcacg gtcgagcgcc cgggtgcggc ctggtggacc tcggcgaaca cctgctgcat
      361 gcgcttgctg cggccgatga cgttctcgat cgtgtacttg cccttcagct cggtggcgag
      421 gcgccggttc tcgacacgca ggttctcctg ggcgtgctcg accatgcggt gcagccggat
      481 ggtctgaccg atcaggttgc cgatcatcat caggaagcgc aggtcggtgt cgaagcggct
      541 ggccggttcg ccttcggtgc gccggtcgac gctcagcacg ccgacgacgt tggtgccttc
      601 gcgcagcggc acgccgagaa aggcgacgat ctcgccgggc tgcggccgtt cggcggtgcg
      661 gtgcaggtac agcggttcat tgttgatgtc gggaacgacg atcggcatgc cgctcttgaa
      721 gatgcggccg atgatgcctt cgccggtgcg gaactgcgcg cgcttcgcct cgtccggcga
      781 caggccggtt tcggcgacga cctgcaggtc atgaccgtcg ccggcactga ggcagaccat
      841 cgcgcggcgc acgtgcaggt gtgttgccag caggttcagc acgtcgtgca gcgagcggcg
      901 cagatccagc gagcgggaaa ggatcttgct gatctcgtag atcgtcacca gttccagatg
      961 gtgacggctg ccggcgtgtg cttgggttcc catgtcatcg gcctccgggg ggaatcctgt
     1021 gaaaaccatg cacaggatca atgcaatacc ggagccagac accacacctg tctgatttgt
     1081 aagtagttcg accgtaatcg tcgggtagcc gacacccgac actgcacctg catggcgcat
     1141 gtcgcatgta gggaatccga caattgcacc agaccggacg ttctgtgggt atttcgccgc
     1201 ttcttctgcc gcatcaatcc cttggaagct gacgcagatc atggcacggc ggttgctcga
     1261 agccccgcaa ccctgcccga acccgagtac tgcgaggtac cgaacaatgg tgatgctgac
     1321 ggacaaagcg gtggacgccg tgcgtggcgt gctgaccgcc cagacggaaa cggtggccgg
     1381 cctgcgggtg ctcgtgaaga ccggcggctg cgccggctac gaatacgcgc tcggcctcga
     1441 agtcgagccg gccgccgatg atgcggtgct cgcctatggc gatgacgtga aggtgttcgt
     1501 cgatccggtc agcctgccgc tgctcgatgg cgtgacggtc gacttcgtcg aggacatcac
     1561 cggcgtcggc ttccgcttcg acaaccccaa tgccgtgaag acctgcggct gcggctcttc
     1621 cttctcgacg gacgccaacg cgcccggcgg cacctgctcc agcaagaggg cgtgacggcc
     1681 atgtgggact actcggacaa ggtcaaggat tacttcttca acccggtcaa cgccggcgtg
     1741 ctcgaagaag ccaatgccgt cggcgacgtc ggttcgatct cctgcggcga tgcgctgcgc
     1801 ctgatgctga aggtcgatcc cgagaccgac gtcatcctcg atgcgcgctt ccagactttc
     1861 ggctgcggtt cggcgatcgc ctccagctcg gcgctgaccg agatgatcaa gggcatgacg
     1921 ctcgatcagg cgctgaaggt cacgaaccag gacatcgccg cctacctcga cggcctgcca
     1981 cccgagaaga tgcactgctc ggtcatgggc gccgaggccc tgcacgccgc gatcgcgaat
     2041 taccgcggcg aggagtggaa ggatgaccac gaggaaggcg agctggtctg caagtgcttc
     2101 ggcgtcgatg cggcgctgat cgagcgcacg gtgcgcgaca acggtctcgt caccgtcgag
     2161 caggtcgcga actacaccaa ggccggcggc ggctgctgct cctgccacga gaagatcgag
     2221 gacatcctga tcgccgtcaa cggcgagctg gccgcgaccg cgaagccggc gatcgtgcag
     2281 cagccgaagc cgcgcacgct ggtgcagatc gcgccgctga agcccgaacc ggccccggcc
     2341 ccggccaagc cggcgatgac gatggtcagg aagatccagc tcatcgagaa ggtgctggag
     2401 gacatccgcc cgtcgctgaa gatggacggc ggcaacgccg agctgatcga tgtcgacggc
     2461 gacaccgtct atctgcacct gaccggcgcc tgcatgggct gcagttcctc gggcctgacc
     2521 atcgccggca tccaggagcg tctgatgctt gccttcggca agccgatgcg cgtggtgccg
     2581 agcaagccga ccgcggaggt ctgaagccat gaacgccccg gtctacctcg acaacaacgc
     2641 cacgacccgc gtcgatccgg ccgtgctggc cgagatgctg ccgttcttca ccgagctgtt
     2701 cggcaacccg tcgtcgatgc accactacgc cgccggcgtc gccgaggccg tgcagctcgc
     2761 gcgccgtcag gtgcagtcgc tgctcggtgc gaagcacgac tcggagatca tcttcacgtc
     2821 ctgcggcacc gaggccaaca acaccgcgct gtactcggcg ctcgccgcca accccggccg
     2881 gcgcgagatc atcacctcgg tcgtcgagca tccggccatc ctcgccgtct gcgagcacct
     2941 cgaaaagaac gagggcgtga ccgtgcatcg catcccggtc gacgcgaagg gccggctgga
     3001 gatcggggcg taccaggcgg cgctgtcgga caaggtcgcg atcgtgtcgt tcatgagcgc
     3061 gaacaacgag accggaacac tgttcccggt cgaggaactc gcggtcatgg ccaaggaagt
     3121 cggtgcgctg ttccacaccg acgccgtgca ggcggtcggc aagctgccga tgaacctcgc
     3181 cgattcggcg atcgacatgc tgtcgctgtc cggtcacaag ctgcacgcgc cgaagggcgt
     3241 cggcgcgctg tacctcaagc gcggcacgcg cttccggccg ctgctgcgcg gcgggcatca
     3301 ggagcgcggc cgccgggccg gcaccgagaa cgtcaccggc atcgtcggcc tcggcaaggc
     3361 ggcagagctc gcacaggcgg cgatggagca cgagaacacc gtcgtgcgca cgctgcgtga
     3421 ccggctgcag accggcatcc tgcagtcgat ctcgcactgc ttcgtgaccg gcgacatcga
     3481 gcaccggctg ccgaacacgg tcaacatcgc cttcgagtac atcgagggtg aggcgatcct
     3541 gctgctgctg tcgcgtgccg gcatcgctgc atcgagcggc tcggcctgca cctcgggttc
     3601 gctggagccc tcgcacgtga tgcgggcgat gaacatcccg tacacggccg cgcacggttc
     3661 gacgcgcttc tcgctgtcgc gcgacaccac cgacgccgat gtcgaccgcg tgctggcggt
     3721 gatgcccgag atcatcgccc ggctgcgcca gctctcgccg tactggggca aggacggtcc
     3781 gaaggccacg ccggacttcg cacccgccta cgcctgagca tcggcccggg actcccacac
     3841 cggccccctg cgggcccgat accgactccc atcctgatgg ccccgcacgg gctcggccgt
     3901 accgatctgg tacggccgtc ttttttggcg cggcggcacc tgtccgcacc gtcacccggg
     3961 gccggaacgc gccccggctt cctccgtcta cgctgtcgga cacccccaca gtgcaggagg
     4021 aaacaggcat gacgcgccag atcttcgtca acctgccggt gcgcgatctc gcgcgcaccc
     4081 gcagcttctt cgaatcgctc ggcttcgaat tcaacgaaaa attctccaac gacgaggccg
     4141 cctgcctgat cgtcggcgat ggccgctacg tgatgctgct cgccgagaac ttcttctcga
     4201 ccttcacgca caagccggtc tgcgatgccc gcagcgccac cgaggtgctg gtctgcctgg
     4261 gctgcgacag ccgcgccgag gtcgacgatc tggttgcccg cgcccgcgcc gccggcggca
     4321 gcgtgccgcg cgacgcccag gaccacggtt tcatgtacgc gcacgccttc gaggacccgg
     4381 acggccacat ctgggagctg gtgtacatgg ccgagaacgc cgaggccgcc tgacccggtg
     4441 ggcggaccgg cctcctcgga ccctgcagat cgccgccggc aatgccatgc gcaaaccgcc
     4501 gtatccgtgc atacccgagc gtattgctgc catgcagcat gatcaactta cccaagcaaa
     4561 atactcggct atgctgcaac gcgacatctt cgtggatgtc gcttctcctc ctcctctgtt
     4621 tacgcggtcg ttcgcccccc gcccgaccgc gtttttttat gcccgtcttc cggactccga
     4681 ctgcgctgcc gggcgcatga ccgcccgccg cgcccggatc gcctgctgat ccggcccgcc
     4741 ggcatcgccg gcaggctgag ccatgcttgc agcggcatgc gcaagcgccg gtgaaacccc
     4801 acagccgatg cgcttttccg tcgtgcatcc gccctgctgg cgcagccatg ccgtcctcgc
     4861 ccaccggaga agccc
//
LOCUS       DAOQJH010000101         4874 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_10245_length_4874_cov_4.848931, whole genome shotgun sequence.
ACCESSION   DAOQJH010000101 DAOQJH010000000
VERSION     DAOQJH010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 4874)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4874)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4874
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10245_length_4874_cov_4.848931"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(31..1224)
                     /gene="prpF"
                     /locus_tag="PLQ18_03540"
     CDS             complement(31..1224)
                     /gene="prpF"
                     /locus_tag="PLQ18_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019447399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylaconitate cis-trans isomerase PrpF"
                     /protein_id="HNE00756.1"
                     /translation="MAFAPQIKIPATYIRGGTSKGVFFRLLDLPEACQQPGEARDKLL
                     LRVIGSPDPYGKQIDGMGGATSSTSKTVILAKSSRPDHDVDYLFGQVSIDKAFVDWSG
                     NCGNLSAAVGAFAISGGLVDAARVPKDGMATVRVWQVNIGKTIIAHVPMSNGEVQETG
                     DFELDGVTFPAAEVQLEFLDPADEGEGDAGGSMFPTGNLVDDLEVPGVGTLKATMINA
                     GIPTIFVNAEAIGYTGTELQEAINGDSKALAMFETIRAYGALRMGLIKDVDEATKRQH
                     TPKIAFVAKPVDYVSSSGKTVAAGDVDLLVRALSMGKLHHAMMGTAAVAIGTAAAVPG
                     TLVNLAAGGGERNAVRFGHPSGTLRVGAEAKQVDGQWTVTKAIMSRSARVLMEGWVRV
                     PGDTF"
     gene            complement(1321..3936)
                     /gene="acnD"
                     /locus_tag="PLQ18_03545"
     CDS             complement(1321..3936)
                     /gene="acnD"
                     /locus_tag="PLQ18_03545"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014086758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047456 - 2-methylisocitrate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe/S-dependent 2-methylisocitrate dehydratase
                     AcnD"
                     /protein_id="HNE00757.1"
                     /translation="MNTAYRKLLPGTKLDYFDARAAVDEIKAGAWATLPYTSRVLAEN
                     LVRRCEPAMLRDSLSQLIERKRDLDFPWFPARVVCHDILGQTALVDLAGLRDAIADQG
                     GDPAKVNPVVPTQLIVDHSLAVEYGGFDPEAFDKNRAVEERRNEDRFHFIDWTRTAFQ
                     NVDVIPAGNGIMHQINLEKMSPVIQVRDGVAFPDTCVGTDSHTPHVDALGVIAIGVGG
                     LEAETVMLGRASMMRLPDIVGVRLTGKRQPGITATDIVLALTEFLRKERVVGAYVEFF
                     GEGAESLSIGDRATISNMCPEYGATAAMFYIDGQTIDYLKLTGREPEQVALVEQYAKT
                     LGLWGDALASAEYERVLTFDLSTVVRNMAGPSNPHKRLPTSALAERGIADEAKLAQAR
                     AEEADGLLPDGAVIIAAITSCTNTSNPRNVVAAGLLARKANQLGLTRKPWVKSSFAPG
                     SKVAKLYLEESGLLPELEKLGFGIVAYACTTCNGMSGALDPKIQQEIIDRDLYATAVL
                     SGNRNFDGRIHPYAKQAFLASPPLVVAYALAGTVRFDIEQDALGFDADGRAITLKDLW
                     PSDEEIDAIVASSVKPEQFKQVYIPMFDLGTVEAAKSPLYDWRPQSTYIRRPPYWEGA
                     LAGERTLKGMRPLAVLPDNITTDHLSPSNAILLDSAAGEYLAKMGLPEEDFNSYATHR
                     GDHLTAQRATFANPKLVNEMVVVDGKVKQGSLARVEPDGTVMRMWEAIETYMDRRQPL
                     IIIAGADYGQGSSRDWAAKGVRLAGVEAIVAEGFERIHRTNLIGMGVLPLEFQPGTDR
                     KTLGLDGTETYDVTGERKPRTTLTLVIHRRNGETVEVPVTCRLDTAEEVSIYEAGGVL
                     QRFAQDFLASSSQAA"
     gene            complement(3939..>4874)
                     /gene="prpC"
                     /locus_tag="PLQ18_03550"
     CDS             complement(3939..>4874)
                     /gene="prpC"
                     /locus_tag="PLQ18_03550"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014007509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050440 - 2-methylcitrate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HNE00758.1"
                     /translation="YKAKLKALRGLPLPVKAVLEQIPAAAHPMDVMRTAVSTLGCVLP
                     EKDDHNSAGARDIADRLIASLGSMLLYWYHYSHNGKRIEVETDDESIGGHFLRLLHGE
                     RPSELHVRSMHTSLVLYAEHEFNASTFTGRVIAGTSSDLYSAITGAIGALRGPKHGGA
                     NEVAFEIQQRYDNPEEAEADIRERVERKEVVIGFGHPVYTISDPRNKVIKEVARTLAA
                     DAENFKLFDIAERLETVMWDAKKMFPNLDWFSAVSYHLMGVPTEMFTPLFVIARTTGW
                     AAHVIEQREDGKIIRPSANYIGPEDRPFVAIEDRK"
BASE COUNT          744 a         1582 c         1683 g          865 t
ORIGIN      
        1 ccctgtttta tgtaaaacgt cagacgccga tcagaatgta tcccccggca cccgcaccca
       61 tccttccatc agcacccgcg cactgcggct catgatcgcc ttggtcacgg tccactgacc
      121 gtcgacctgc tttgcctcgg cgccgacgcg cagcgtgccg gacgggtggc cgaagcgtac
      181 ggcgttgcgt tcgccgccac cggccgcgag gttgaccagc gtgccgggaa ccgcggcggc
      241 ggtgccgatg gcgacggcgg ccgtgcccat catcgcgtgg tgcagtttgc ccatcgacag
      301 cgcgcgcacc agcagatcga catcgccggc tgcgaccgtc ttgccgctcg atgacacgta
      361 atcaaccggc ttcgcgacaa aggcgatctt cggcgtgtgc tggcgcttcg tggcttcatc
      421 gacatccttg atcaggccca tgcgcagcgc cccgtaggcg cggatggtct cgaacatcgc
      481 cagtgccttc gaatcgccgt tgatggcctc ctgcaattcg gtgccggtgt aaccgatggc
      541 ttcggcattg acgaagatcg tcggaatgcc ggcattgatc atcgtcgcct tcagcgtgcc
      601 gacgccgggc acttcaaggt catcgacgag attgccggtc gggaacatcg aaccgccggc
      661 atcgccttcg ccttcatcgg ccggatcgag gaattcgagc tgcacttcgg cggccgggaa
      721 ggtcacgccg tcgagttcga aatcgccggt ttcctgcact tcgccgttgc tcatcggcac
      781 gtgggcgatg atggtcttgc cgatgttgac ctgccagacg cgcacggtgg ccatgccgtc
      841 cttcggcacg cgcgccgcat cgacgaggcc gccgctgatt gcaaacgcgc cgaccgcggc
      901 cgacagattg ccgcagttgc cgctccagtc gacaaaggcc ttgtcgatcg acacctgacc
      961 gaacaggtaa tcgacatcgt gatccgggcg gctgctcttt gcgaggatca cggtcttgct
     1021 ggtgctggag gtcgccccgc ccatgccgtc gatctgcttg ccgtaggggt cggggctgcc
     1081 gatcacgcgc agcagcagct tgtcgcgcgc ctcgccgggc tgctggcagg cttcgggcaa
     1141 atccagcaga cggaagaaca cgcccttgct ggtgccgccg cggatgtagg tggcgggaat
     1201 cttgatctgg ggtgcgaagg ccatgatggc tccctgaaag caatgacggg gcggcacgtc
     1261 gccgcgccgc cccgtcggga agaggatgcc gggcctgatg cacaggcccg gacgcccggc
     1321 tcaggctgcc tgcgaagacg acgccaggaa gtcctgcgca aagcgctgca gcacgccgcc
     1381 ggcctcgtag atcgagactt cctcggcggt gtcgagccgg caggtgaccg ggacttcgac
     1441 ggtttcgccg ttgcggcggt ggatcaccag cgtcagcgtc gtgcgcggct tgcgttcgcc
     1501 ggtcacgtcg taggtctcgg tgccgtcgag gccgagcgtc ttgcggtcgg tgccgggctg
     1561 gaattccagc ggcagcacgc ccatgccgat caggttcgtg cggtggatgc gctcgaagcc
     1621 ctcggcgacg atggcctcga cgccggccag acgcacgccc ttggccgccc agtcacgcga
     1681 actgccctga ccgtaatcgg caccggcgat gatgatcagc ggctggcggc gatccatgta
     1741 ggtctcgatc gcctcccaca tgcgcatgac cgtgccgtcc ggctcgacgc gcgcgagcga
     1801 tccctgcttg accttgccat cgacgacgac catctcgttg acgagcttcg ggttcgcgaa
     1861 ggtcgcgcgc tgggcggtca ggtgatcgcc gcggtgcgtc gcgtaggaat tgaagtcctc
     1921 ctccggcagg cccatcttcg ccaggtattc accggcggcg ctgtcgagca ggatcgcgtt
     1981 cgacggcgac aggtgatcgg tcgtgatgtt gtccggcagc accgcgagcg gacgcatgcc
     2041 cttgagcgtg cgctcgccgg ccagcgcgcc ttcccagtac ggcggacggc ggatgtaggt
     2101 cgactgcgga cgccagtcgt acagcgggct cttggcggcc tcgacggtgc cgaggtcgaa
     2161 catcgggatg tagacctgct tgaactgctc gggcttgacc gagctggcca cgatggcgtc
     2221 gatctcctcg tcgctcggcc agagatcctt cagcgtgatc gccctgccat cggcatcgaa
     2281 accgagcgcg tcctgctcga tgtcgaaacg cacggtgccg gcgagcgcgt aggcgacgac
     2341 cagcggcggc gaggcgagga aggcctgctt ggcgtacggg tggatgcggc cgtcgaagtt
     2401 gcggttgccg gagagcacgg cggtcgcgta caggtcgcgg tcgatgatct cctgctggat
     2461 cttcggatcg agcgcgccgg acatgccgtt acacgtcgtg caggcatagg cgacgatgcc
     2521 gaagccgagc ttttcgagtt ccggcagcag accggactct tcgagataca gcttcgcgac
     2581 cttcgagccc ggcgcgaacg aggacttcac ccagggcttg cgcgtcaggc cgagctgatt
     2641 ggccttgcgc gccagcaggc cggcggcaac gacgttgcgc gggttgctgg tgttggtgca
     2701 gctcgtgatc gccgcgatga tgaccgcgcc atcgggcagc aggccgtcgg cctcctcggc
     2761 cctcgcctgc gcgagcttgg cttcgtcggc gatgccgcgc tcggcgagcg ccgaggtcgg
     2821 caggcgcttg tgcgggttcg agggccccgc catgttgcgc acgaccgtcg acaggtcaaa
     2881 cgtcagcacg cgctcgtact cggcgcttgc cagcgcatcg ccccagaggc cgagcgtctt
     2941 cgcgtactgc tcgaccagcg ccacctgctc cggctcgcgg ccggtcagct tcaggtaatc
     3001 gatggtctgg ccgtcgatgt agaacatcgc ggcggtcgca ccgtactcgg ggcacatgtt
     3061 cgagatggtc gcgcgatcgc cgatcgacag gctctcggcg ccttcgccga agaactcgac
     3121 gtaggccccg accacgcgct ccttgcgcag gaactcggtc agcgccagca cgatgtcggt
     3181 ggcggtgatg ccgggctggc gcttgccggt gagcctgacg ccgacgatgt cgggcaggcg
     3241 catcatcgac gcgcgcccga gcatgacggt ctcggcttcg agtccgccga cgccgatggc
     3301 gatgacgccg agcgcatcga cgtgcggcgt gtggctgtcg gtgccgacgc aggtgtccgg
     3361 gaaggcgacg ccgtcacgga cctggatcac cggcgacatc ttctccagat tgatctggtg
     3421 catgatgccg ttgccggccg ggatcacgtc gacgttctgg aaggccgtgc gcgtccagtc
     3481 gatgaagtgg aagcggtcct cgttgcgacg ctcctcgacc gcgcggttct tgtcgaaggc
     3541 ctcggggtcg aagccgccgt actcgacggc gagcgagtga tcgacgatca gctgcgtcgg
     3601 caccaccgga ttgaccttgg ccggatcgcc gccctgatcg gcgatcgcgt cgcgcaggcc
     3661 ggcgaggtcg acgagcgcgg tctggccgag gatgtcgtgg cagacgacgc gcgccgggaa
     3721 ccacgggaag tcgaggtcgc gtttgcgctc gatcagctgg ctgagcgaat cgcgcagcat
     3781 cgccggttcg cagcggcgca cgaggttctc ggccagcacg cgcgaggtgt agggcagcgt
     3841 cgcccaggcc ccggccttga tctcatcgac ggcggcacgc gcatcgaagt aatccagctt
     3901 cgtgcccggc agcagtttgc gataggcagt gttcatggtc acttgcggtc ctcgatcgcg
     3961 acgaacggcc ggtcttccgg gccgatgtag ttggcgctcg ggcggatgat cttgccgtcc
     4021 tcgcgctgct cgatgacgtg tgcggcccag ccggtggtgc gcgcgatcac gaacagcggc
     4081 gtgaacatct ccgtcggcac gcccatcagg tggtacgaga cggccgagaa ccagtcgagg
     4141 ttcgggaaca tcttcttggc gtcccacatg acggtttcga gccgctcggc gatgtcgaac
     4201 agcttgaagt tctcggcatc ggcggcgagc gtgcgcgcga cttccttgat caccttgttg
     4261 cgcggatcgg agatcgtgta gaccgggtga ccgaagccga tgacgacttc cttgcgctcg
     4321 acgcgctcgc ggatgtcggc ctcggcctct tccgggttgt cgtagcgctg ctggatctcg
     4381 aaggcgacct cgttggcacc gccgtgcttc ggaccgcgca gcgcgccgat cgcgccggtg
     4441 atcgccgagt acaggtcgga cgaggtgccg gcgatgacgc ggccggtgaa ggtcgaggcg
     4501 ttgaactcgt gctcggcgta cagcaccagc gaagtgtgca tcgagcgcac gtgcagctcg
     4561 ctcgggcgct cgccgtgcag caggcgcagg aagtggccgc cgatcgactc gtcgtcggtc
     4621 tcgacctcga tgcgcttgcc gttgtggctg tagtggtacc agtacagcag catcgagccg
     4681 agcgaggcga tcaggcggtc ggcgatgtcg cgcgcaccgg cggagttgtg atcgtccttc
     4741 tccggcagca cgcagccgag cgtcgagacg gccgtgcgca tgacatccat cgggtgcgcg
     4801 gcggccggaa tctgctccag caccgccttg accggcagcg gcaggccgcg cagcgccttg
     4861 agcttggcct tgta
//
LOCUS       DAOQJH010000102         4851 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_10327_length_4851_cov_4.141993, whole genome shotgun sequence.
ACCESSION   DAOQJH010000102 DAOQJH010000000
VERSION     DAOQJH010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 4851)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4851)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4851
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10327_length_4851_cov_4.141993"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..989
                     /locus_tag="PLQ18_03555"
     CDS             <1..989
                     /locus_tag="PLQ18_03555"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing response regulator"
                     /protein_id="HNE00759.1"
                     /translation="LARAGCRSRLILVSGCERRVLQAALRLAQGFGLDVLGELQKPVS
                     VAALTALLLQPGMPPARVQPLQPVTLDELHAALAENRFTVHYQPQVALHDGRWCGVEG
                     LVRWCHPQHGLLLPDSFVPLVERGGLGLELTRCVLECALADLAHLHGLLPGGRLPGLS
                     VNLPGEALFDVSLPEQLLALVDAAAWQHDAITFEITETSLAREPVRALDILTRLRLKG
                     VQLSIDDFGTGYSSFGQLHHLPFSELKIDLGFVRVADRDPVARAIVENSIRLGRQLGL
                     TVVAEGVESATLWHWLREQGCDLAQGYLVSRPMPAEQLPAWYAAWGAPACPA"
     gene            1384..1605
                     /locus_tag="PLQ18_03560"
     CDS             1384..1605
                     /locus_tag="PLQ18_03560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE00760.1"
                     /translation="MFPADMPDAVCACSRPFLQGHASAAEPVFPVDRDLEHLYVQAAH
                     RARLLGQAQFEAIARHAHPDPLRSHRHAD"
     gene            complement(1635..2213)
                     /locus_tag="PLQ18_03565"
     CDS             complement(1635..2213)
                     /locus_tag="PLQ18_03565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE00761.1"
                     /translation="MTEPRPDTPDCASPPCLAHELDPLWSGRAGYAVPLRAPVRAEHS
                     YVQRLCATPAQVLPLLCPVRETDWIADWRPQYVISASGLAEPDCVFATPAPAGGSALW
                     YVTRHDAVQGYVEMVRFVPEHTACRLEIWLQADGDSACLATVRYRLTSLGSVAGDAAV
                     AAYTAEHYLAFMRDWERRLNHWLRTGRCLPAD"
     gene            complement(2242..2622)
                     /locus_tag="PLQ18_03570"
     CDS             complement(2242..2622)
                     /locus_tag="PLQ18_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004359696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding protein"
                     /protein_id="HNE00762.1"
                     /translation="MSRTDRTLPLVYSCSGCSSAAQLANHVALTLDRRGLAEMSCIAG
                     VGGGVPALVRVAQGTRPVIALDGCALACVRHCLARAGVSAGTYHLLSDHGVRKRHGAD
                     FDAADAARVLEIVIAGLPQAATAA"
     gene            2995..>4851
                     /locus_tag="PLQ18_03575"
     CDS             2995..>4851
                     /locus_tag="PLQ18_03575"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HNE00763.1"
                     /translation="MSELSQTPAGARRLPLARYLWQSYLRAAIIPLLVIELGFIGVYW
                     VSSFITYERNAEALGAISREDLARTAQREADAIAEKLGAVGDLTRVFAAETARALRTP
                     APAAATDDDARHAYGADGVYYTTREGVSAGFYSGIVPVGPAERDKVRRTAAIDPLMQA
                     IKAANPLVAQLYLNTHDSYNRIHPYFEVLTQYPAKMDIPSYNFYYEADAAHDPERRAV
                     WTDAYVDPAGSGWMVSSIAPVYGGADGNFLEGVVGIDVTVDTIVRRVLNLQLPWQGYA
                     LLVGRDGTILALPPRGERDFGLKELTAHSYDEAIRADTFKPDDFNVHKRADLAVLGRA
                     LKAGGDGVVELALGSGRTLAGWSTVAGPGWQLVVLAPEHEILAGATALRERLSLVGWL
                     MIASLMLFYVGFLIYLRSRARAMTARVAAPLGDIEALIRRIGGGDYEQSEPDYRIAEL
                     QTVGTHLVDMGRRLGAAYAAMVEAERSVRAALDKERELGEVQRQFINVVSHEFRTPLT
                     IIDGVAQTFERRADRLGPDKIIERARSLRASVRRLVDVLESALSFSRLESSQLTHAFG
                     PVAFAAVLQEVVQTETVAYPQHRFEIDGALPATVHGDAGLLRVMLSAVLDNAA"
BASE COUNT          696 a         1722 c         1700 g          733 t
ORIGIN      
        1 aactcgcacg tgccggctgt cgctccaggc tgatcctggt cagcggctgc gagcgcaggg
       61 tgctgcaggc ggcgctgcgg ctcgcgcagg ggttcggact cgatgtgctc ggcgaactgc
      121 agaagccggt gtccgtcgcc gcgctgaccg cgctgctgct gcagccgggc atgccgccgg
      181 cccgggtgca gccgctgcag ccggtgacgc tggacgagct gcacgcggcg ctcgccgaga
      241 accgcttcac cgtgcactac cagccgcagg tggcgctgca cgacgggcgc tggtgcgggg
      301 tcgagggact ggtgcgctgg tgccacccgc agcacggcct gctgctgccc gacagcttcg
      361 tgccgctcgt cgaacggggc ggactcgggc tggaactcac ccgctgcgtg ctcgaatgcg
      421 ccctcgccga tcttgcgcac ctgcacggcc tgctgccggg cggccgcctg ccgggactgt
      481 cggtcaacct gccgggcgag gccttgttcg acgtgtcgct gcccgagcag ctgctcgcgc
      541 tggtcgatgc ggcggcctgg cagcacgacg cgatcacctt cgagatcacc gagacctcac
      601 tggcccgcga gccggtccgg gccctcgaca tcctgacgcg cctgcggctg aagggcgtgc
      661 agctgtcgat cgacgacttc ggcaccggtt actcgtcgtt cgggcagctg catcacctgc
      721 cgttctcgga gctgaagatc gatctcggct tcgtgcgcgt cgccgatcgc gatccggtgg
      781 cgcgggcgat cgtggaaaac tcgatccggc tcgggcgcca gctcgggctg accgtggtcg
      841 ccgaaggcgt cgagagcgcg acgctgtggc actggctgcg cgagcagggc tgcgatctcg
      901 cgcagggcta cctggtctcg cggccgatgc cggccgagca gctgccggcc tggtacgcgg
      961 cctggggggc accggcctgt ccggcctgat cagcggcggc gcaacaagcc gattttgttg
     1021 ttgtaaatac aaaacggctt ttctttcttg caacaatgcc gttgcctgct acaacaggga
     1081 ccgtccgcat gcgtcggcaa gaacgcggac ggccgaagcc tcgccgtgcg tcagcccctg
     1141 cacgtcccgc gcttcctgca atcttgcatt tgttgtgcat tcatccgcat ttgccctgca
     1201 tctgccggat tgctgcacgc gcaagtcggg aacaggatcg gccgatatcc ccgtcagcag
     1261 cgcccgccgg ctggtccgtc gcagcgtctg catgccggtt cgtggcggcc gaacgagcgc
     1321 caccggccgg tgtccgttct tacggagtga cccctggagg cttgcacgat ggatgctcag
     1381 ccgatgttcc ccgccgacat gcccgatgcg gtctgcgcct gttcccgccc gttcctgcag
     1441 gggcacgcca gcgctgccga gccggtgttc ccggtcgatc gcgacctcga acacctgtac
     1501 gtgcaggccg cacatcgcgc ccgcctgctc ggtcaggcgc agttcgaggc gatcgcgcgt
     1561 catgcgcatc ccgatccgct gcgcagccac cggcatgcgg actgagccgg ctgccggcag
     1621 cggggcgcgg ggcgtcagtc cgcgggcagg cagcggccgg tccgcagcca gtggttcagc
     1681 cgtcgctccc agtcgcgcat gaacgccagg tagtgctccg cggtataggc cgcgaccgca
     1741 gcatcgccgg caacgctgcc gaggctggtc agccggtagc ggaccgtcgc gagacaggct
     1801 gaatcgccat cggcctgcag ccagatctcc agccggcagg ccgtgtgctc cggcacgaag
     1861 cgcaccatct ccacgtaacc ctgcactgca tcgtggcggg tcacgtacca cagcgcgctg
     1921 cccccggccg gggccggtgt cgcgaacacg cagtcgggct cggcgaggcc cgaggcactg
     1981 atcacgtact gcggacgcca gtccgcgatc cagtccgtct cccgtaccgg acagagcagc
     2041 ggcagcacct gcgccggtgt ggcgcagagc cgctgcacgt agctgtgctc ggcgcgcacc
     2101 ggcgcgcgca ggggcacggc atagccggcc cggccgctcc agagcgggtc gagttcgtgc
     2161 gccaggcacg gcggcgaggc gcagtcgggg gtgtcggggc gcggttcggt catgggcagg
     2221 cacctggctg gcacggccgg ttcaggctgc ggtggcagcc tgcggcaggc cggcaatgac
     2281 gatttcgagc acgcgtgcgg cgtcggcggc atcgaagtct gcaccgtggc gcttgcgcac
     2341 gccgtgatcg gacagcagat gataggtgcc ggcgctgacg ccggcccggg cgaggcaatg
     2401 gcgcacgcag gcgagcgcgc agccatcgag cgcgatgacc ggtcgcgtgc cctgcgcgac
     2461 gcgcaccagc gccggcacgc cgccgccgac tccggcgatg cacgacatct cggcgagccc
     2521 gcggcgatcg agcgtcagcg cgacgtggtt ggcgagttgc gcagcactcg aacagcccga
     2581 gcagctgtag accaggggca gggtgcggtc ggtacgggac atcggtaacg cctcatcgtg
     2641 atgtgaaata tccgcaaggc tattttttgc atcgcgttcc gcccttgaga tgcatcaagc
     2701 cggtaccgct gcctgtccgc ccccggcacc cgcttcaccc gccggatgcg cccggccggc
     2761 cgcggacggg gcggtcaatc gtcacaagtt gcgggaatcc ggccggattc ccggggttcg
     2821 ccttgtccgt cgtccggtca cgtggcacct tgccgcgcct gaaataccgg ccggtccgca
     2881 gccggcgctg ccggcagcgg cagcgcggcc ccaggccgga ggaaccatcc ggcgctgcgc
     2941 accaggcgca gcgccggatt catacccgca cgggcaggac gggctgtacc gaccatgagt
     3001 gaactttccc agacccccgc cggtgcccgg cgtctgccgc tggcgcgcta cctgtggcag
     3061 tcctatctgc gcgccgcgat catcccgctg ctggtgatcg aactcggctt catcggcgtg
     3121 tactgggtgt cgagcttcat cacctacgag cgcaacgccg aggcactcgg ggcgatctcg
     3181 cgcgaggatc tcgcccgcac cgcgcagcgc gaggccgatg cgatcgccga aaagctcggc
     3241 gcggtcggtg acctgacgcg cgtgttcgcg gccgaaaccg cgcgcgccct gcgcacgccg
     3301 gccccggcgg cggcgaccga tgacgatgcc cgccacgcct acggcgcgga tggcgtctac
     3361 tacacgacgc gcgagggcgt ctcggccggc ttctacagcg gcatcgtgcc ggtcggcccg
     3421 gccgagcgcg acaaggtacg gcgcacggcg gcgatcgatc cgctgatgca ggcgatcaag
     3481 gcggccaacc cgctggtcgc gcagctctac ctgaacacgc acgacagcta caaccgcatc
     3541 catccgtact tcgaggtgct gacgcagtac ccggcgaaga tggacatccc cagttacaac
     3601 ttctactacg aggccgacgc cgcccacgac cccgaacggc gcgcggtctg gaccgacgcc
     3661 tacgtcgatc cggccggcag cggctggatg gtgtcgtcga tcgcaccggt ctacggcggc
     3721 gccgacggca acttcctcga aggcgtggtc ggcatcgacg tgaccgtcga caccatcgtc
     3781 aggcgcgtgc tgaacctgca gctgccctgg cagggctacg cgctgctggt cgggcgcgac
     3841 ggcacgatcc tggcgctgcc gccgcgcggc gaacgggatt tcggcctgaa ggagctgacc
     3901 gcgcacagct acgacgaggc gatccgcgcc gacaccttca agcccgatga cttcaacgtc
     3961 cacaagcgcg ccgacctcgc cgtgctcggc cgggcgctga aggccggcgg ggacggtgtc
     4021 gtcgaactcg cgctcggcag cggccgcacg ctggccggct ggtcgacggt ggccgggccc
     4081 ggctggcagc tggtggtgct cgcaccggag cacgagatcc tcgccggggc cacggcgctg
     4141 cgcgagcggc tgtcgctggt cggctggctg atgatcgcga gcctgatgct gttctacgtc
     4201 ggcttcctga tctacctgcg cagccgcgcc cgggcgatga ccgcgcgcgt ggccgcgccg
     4261 ctcggcgaca tcgaggcgct gatccggcgc atcggcggcg gcgactacga gcagtccgag
     4321 cccgactacc gcattgccga gctgcagacc gtcggcacgc acctggtcga catgggccgg
     4381 cgtctcggtg ccgcctacgc ggcgatggtc gaggccgaac gcagcgtgcg cgccgcgctc
     4441 gacaaggagc gcgaactcgg cgaggtgcag cgtcagttca tcaacgtcgt ctcgcacgag
     4501 ttccgcacgc cgctgaccat catcgacggc gtcgcgcaga ccttcgagcg gcgtgccgac
     4561 cgcctcggcc ccgacaagat catcgaacgc gcgcgcagcc tgcgcgcctc ggtgcgccgg
     4621 ctggtcgacg tgctcgaaag cgcgctgtcg ttctcgcgcc tggagagcag ccagctcacg
     4681 catgccttcg ggccggtggc cttcgcggcc gtgctgcagg aggtcgtgca gaccgagacc
     4741 gtcgcctatc cgcagcaccg cttcgagatc gacggcgcgc tgccggcgac ggtgcacggc
     4801 gatgccgggc tgctgcgcgt catgctgtcg gccgtgctcg acaacgccgc g
//
LOCUS       DAOQJH010000103         4850 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_10332_length_4850_cov_3.578311, whole genome shotgun sequence.
ACCESSION   DAOQJH010000103 DAOQJH010000000
VERSION     DAOQJH010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 4850)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4850)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4850
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10332_length_4850_cov_3.578311"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..624)
                     /locus_tag="PLQ18_03580"
     CDS             complement(<1..624)
                     /locus_tag="PLQ18_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006786400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HNE00764.1"
                     /translation="MAAKDVKFGDDARSRMVRGINILANAVKVTLGPKGRNVVLDKAF
                     GAPTVTKDGVSVAKEIELSDKFENMGAQMVKEVSSKTSDVAGDGTTTATVLAQAIVRE
                     GMKSVSAGMNPMDLKRGIDKAVQAAVGELAKLSKPCADDKAIAQVGAISANGDEQIGR
                     IIADAMAKVGKEGVITVEDGSGLENQLDVVEGMQFDRGYLSPYFINNQ"
     gene            complement(727..1017)
                     /gene="groES"
                     /locus_tag="PLQ18_03585"
     CDS             complement(727..1017)
                     /gene="groES"
                     /locus_tag="PLQ18_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008316614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HNE00765.1"
                     /translation="MSLRPLHDRVIVKRVEEETKSAGGIVLPGNAAEKPSRGTVLAIG
                     RGKILENGDVRPLDVKVGDHVLFGKYSGSEVKIDGDEVLVMREDEIMAVIEK"
     gene            complement(1224..1673)
                     /locus_tag="PLQ18_03590"
     CDS             complement(1224..1673)
                     /locus_tag="PLQ18_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018954630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HNE00766.1"
                     /translation="MKLPAVLFVLFVALPIVEIYVLVRVGSVIGALPTIALVIGLSAF
                     GAWLVRHEGLGVLQRVRTALDAGQLPARELLDGALILIGGVLLLTPGFVTDVFGLCCL
                     LPPVRAQLIRLLARHAIVVAGRRGPPGGGAPGARGPQTLEGEYRRED"
     gene            1831..2166
                     /gene="cutA"
                     /locus_tag="PLQ18_03595"
     CDS             1831..2166
                     /gene="cutA"
                     /locus_tag="PLQ18_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020506412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HNE00767.1"
                     /translation="MDTPCQLVLCTCPDADSAGHIATVLVGEGLAACANLVPGVVSVY
                     RWQGAVQRDAEVLMLLKTRADAFEALAARLRELHPYTVPEIIAVGIEAGSPAYIGWLL
                     AETRAGGPA"
     gene            2166..4043
                     /gene="dsbD"
                     /locus_tag="PLQ18_03600"
     CDS             2166..4043
                     /gene="dsbD"
                     /locus_tag="PLQ18_03600"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF001419.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047134 - protein-disulfide reductase
                     (NAD(P)) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HNE00768.1"
                     /translation="MSRFAVLLRRWLPVACMLLVAGLPPAMAIDLFGKKPSADDLLDA
                     EQAFFVEAPAVEPGAVRLRWTIADGYYLYRARLKVEPEGALPPGITIAGVDTPAGEIK
                     DDPSFGRTEVWHEAAIATVRLTRSGAGLGAQPLTLRAEWQGCNEPVGVCYPPQSKTWT
                     LELPAATATPMSATAAAAPAGPGATPAATAPAPEPEQDRMARMLRNERFLALPAFFGF
                     GLLLSFTPCVFPMVPILSGLIVGRGRHTTRGHAFGLSVVYVLAMASTDALAGVVAASF
                     GENLQAAFQHPGVLIAFALLFVALALSMFGLYQLQLPGALQARLSEISGHQRGGDYAG
                     VAIMGLLSALIVGPCVAPPLIGALAVIASTGDRLLGGAALFAMSLGMGVPLLAIGASA
                     GHFLPRAGHWMDKVKAVFGVALLAVALVMLDRVLPAALIMLAWAVLLVVSATYMGALQ
                     PIPQGAPWWRTLIKGLGLVLLMQGALLLIGVAAGARDPLRPLAGIVAAGGNSEAAHGL
                     AFRRIVSLAELDAEIAAAGGRPVLLDFYADWCVACKELERDTFPDPQVRTRLAGFVRL
                     QADVTANSADDRALLRRYGLVGPPAILIFGADGRERTELRTVGFVGPEPFAELLGRVG
                     S"
     gene            4246..4698
                     /gene="aroQ"
                     /locus_tag="PLQ18_03605"
     CDS             4246..4698
                     /gene="aroQ"
                     /locus_tag="PLQ18_03605"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010221695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HNE00769.1"
                     /translation="MAQLLVLNGPNLNLLGTREPATYGAQTLADIRQNCEALAAAQGH
                     ALDFFQSNAEHALIERIHAARTDGTDFIVINPAAYTHTSVALRDALLAVAIPFIEVHL
                     SNVHAREPFRHHSYFSDVALGVICGLGAQGYELALAAADRHLAARKPA"
     gene            4706..>4850
                     /locus_tag="PLQ18_03610"
     CDS             4706..>4850
                     /locus_tag="PLQ18_03610"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253534.1"
                     /note="composes the biotin carboxyl carrier protein
                     subunit of the acetyl-CoA carboxylase complex, the enzyme
                     that catalyzes the carboxylation of acetyl-CoA to
                     malonyl-CoA, which in turn controls the rate of fatty acid
                     metabolism; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HNE00770.1"
                     /translation="MDIRKIKKLIELLEESGIAEIEIKEGEEAVRISRHPAASAATPV
                     LMQA"
BASE COUNT          691 a         1728 c         1666 g          765 t
ORIGIN      
        1 ctggttgttg atgaagtacg gcgacaggta gccgcgatcg aactgcatgc cctcgacgac
       61 atcgagctgg ttctccaggc ccgaaccgtc ctcgacggtg atcacgcctt ccttgccgac
      121 cttggccatc gcgtcggcga tgatgcggcc gatctgctcg tcgccgttgg cggagatcgc
      181 gccgacctgg gcgatcgcct tgtcatcggc gcagggcttg gacagcttgg cgagttcgcc
      241 gacggcggcc tgcacggcct tgtcgatgcc gcgcttgagg tccatcgggt tcatgccggc
      301 cgagaccgac ttcatgcctt cgcggacgat ggcctgcgcc agcacggtcg cggtcgtcgt
      361 gccgtcgccg gcgacatcgg aagtcttcga ggagacttcc ttgaccatct gcgcgcccat
      421 gttctcgaac ttgtcggaca gttcgatttc cttggcgacg gagacgccgt ccttggtcac
      481 ggtcggggcg ccgaaggcct tgtcgagcac gacgttgcgg cccttcggac cgagggtgac
      541 cttgaccgcg ttggccagga tattgatgcc gcgcaccatg cgcgaacggg cgtcatcacc
      601 gaactttacg tctttggcag ccattgcttc ggaaacctcg ggaattctgg atggaattca
      661 ggaaatgaat tctggaacgg acgaacgggt gatcaggcag tcaggcgatc ggcggcgggc
      721 gccggatcac ttctcgatga cggccatgat ctcgtcctcg cgcatcacga ggacttcgtc
      781 gccgtcgatc ttgacttcgc tgcccgaata cttgccgaac aggacgtggt caccgacctt
      841 cacgtcgagc gggcgcacgt cgccgttctc gagaatcttg ccgcgaccga tcgcgagcac
      901 ggtgccgcgc gacggcttct cggctgcgtt gccgggcagg acgatgccac cggcggactt
      961 ggtctcttcc tcgacacgct tgacgatgac gcggtcatgc agggggcgca ggctcatgcg
     1021 aatctcctca gaaatacgag ggttgggttg tggaggacgg gcggtgccgg acttgttagc
     1081 actcaccttg agcgagtgct gatattaggc agcgactgtc ccctgtcaag ggacgcagcc
     1141 ggaaatgggg ccgcacaggc ggctttcaag agcgccggga atgggctgcg gaccccggac
     1201 gccgccgtcc ccgggccggc gcgtcagtcc tcgcgccggt actcgccttc cagggtctgc
     1261 ggtccgcgcg cgcccggtgc gccgccgccg ggcgggccgc ggcggccggc gacgacgatg
     1321 gcgtggcgcg cgagcagccg gatcagctgc gcgcgcaccg gcggcagcag gcagcagagc
     1381 ccgaagacat cggtcacgaa gcccggcgtc agcagcagca cgccgccgat caggatcagc
     1441 gcgccgtcga gcagttcgcg cgccggcagc tggccggcat cgagggccgt gcgcacgcgc
     1501 tgcagcacgc cgaggccctc atggcgcacc agccaggcac cgaaggccga caggccgatg
     1561 accagcgcga tggtcggcag cgcaccgatc accgagccga cgcgcaccag cacgtagatc
     1621 tcgacgatcg gcagcgcgac gaacagcacg aacaacacgg cgggcagctt catgacgggg
     1681 ggctcctgcg cgcgatggcc ggatgcgcgc gctgtgcagg ggtgagcgtg gagggacgag
     1741 ccggccggta cgcggcccat ggccgcaacg gcaccttata cttgccggct gcgcctgccg
     1801 accctctgcc gccgtgaagg tgcccatccc gtggatacgc cctgccagct cgtgctctgc
     1861 acctgccccg acgccgacag cgccggacac atcgccaccg tgctcgtcgg ggaaggcctc
     1921 gccgcctgcg cgaacctcgt gccgggcgtc gtgtccgtat accgctggca gggggccgtg
     1981 cagcgcgatg ccgaagtgct gatgctgctg aagacgcgcg ccgacgcctt cgaggccctt
     2041 gccgcccgcc tgcgcgaact gcatccgtac acggtgccgg aaatcatcgc ggtcggcatc
     2101 gaagccggct cgcctgcgta catcgggtgg ctgctcgccg aaacccgcgc cggaggtcca
     2161 gcctgatgtc ccgtttcgcc gtcctgctgc gccgctggct gccggtggcc tgcatgctgc
     2221 tcgtggccgg cctgccgccc gccatggcca tcgacctgtt cggcaagaag ccgtcggccg
     2281 acgacctgct cgacgccgag caggccttct tcgtcgaagc tccggcggtc gagcccggcg
     2341 ccgtgcgcct gcgctggacc atcgccgatg gctactacct gtaccgcgcg cggctgaagg
     2401 tcgagcccga aggcgcgctg ccgcccggca tcacgatcgc cggcgtcgac acgccggccg
     2461 gcgagatcaa ggacgatccg agcttcgggc gcaccgaggt ctggcacgaa gcggcgatcg
     2521 ccaccgtgcg cctgacgcgc agcggcgccg gcctcggcgc gcagccgctc acgctgcgcg
     2581 ccgaatggca gggctgcaac gagccggtcg gcgtctgcta cccgccgcag tcgaagacct
     2641 ggacgctcga actgccggcg gcaacggcca cgccgatgtc cgccacggca gcggcggcgc
     2701 ctgcaggccc gggcgccacg ccggcggcca cggcgccggc gcccgaaccc gaacaggacc
     2761 gcatggcgcg catgctgcgc aacgagcgct tcctggcgct gccggcgttc ttcggcttcg
     2821 ggctgctgct gtcgttcacg ccctgcgtgt tcccgatggt gccgatcctg tcgggcctga
     2881 tcgtcggtcg cggccggcac acgacgcgcg gccacgcctt cggactgtcg gtggtctacg
     2941 tgctggcgat ggcctcgacc gatgcgctcg ccggcgtcgt cgccgcgagc ttcggcgaga
     3001 acctgcaggc ggccttccag cacccgggcg tactgatcgc cttcgcgctg ctgttcgtgg
     3061 cgctcgccct gtcgatgttc gggctgtacc agctgcagct gccgggcgcg ctgcaggcgc
     3121 ggctctcgga gatttccggc caccagcgcg gcggcgacta cgccggcgtc gcgatcatgg
     3181 gcctgctgtc ggcgctgatc gtcggtccct gcgtggcgcc gccgctgatc ggcgcgctgg
     3241 cggtgatcgc gagcaccggc gaccggctgc tcggcggcgc ggcgctgttc gcgatgagcc
     3301 tcggcatggg tgtgccgctg ctcgcgatcg gcgccagcgc cggacacttc ctgccgcgcg
     3361 ccgggcactg gatggacaag gtcaaggcgg tgttcggcgt cgcgctgctc gccgtggcgc
     3421 tggtgatgct cgatcgcgtg ctgccggcgg cgctgatcat gctggcctgg gcggtgctgc
     3481 tggtggtcag cgccacctac atgggcgcgc tgcagccgat cccgcagggc gcgccgtggt
     3541 ggcgcacgct gatcaagggc ctcggcctcg tgctgctgat gcagggcgcg ctgctgctga
     3601 tcggcgtggc ggccggtgcg cgtgatccgc tgcggccgct ggccggcatc gtcgcggccg
     3661 gcggcaattc cgaagccgcg cacggcctgg ccttccggcg catcgtcagc ctcgccgaac
     3721 tcgatgccga gatcgccgcc gccggcggcc ggccggtgct gctcgatttc tatgccgact
     3781 ggtgcgtggc ctgcaaggaa ctggagcgcg acaccttccc ggacccgcag gtgcgcacgc
     3841 ggctcgccgg gttcgtgcgc ctgcaggccg atgtcaccgc caactccgcc gatgaccgcg
     3901 cgctgctgcg ccgctatggt ctggtcgggc cgccggcgat cctgatcttc ggcgccgacg
     3961 gccgcgagcg caccgaactg cgcacggtcg gtttcgtcgg cccggagccg tttgccgaac
     4021 tgctcgggcg cgtcggcagc tgagtccgcg gccgcccgcc gcaccggctc catgaaggaa
     4081 ttgtcacgcg cgattcccgc gcgttcggcg acgttgctcg cgaagtcatg cgcaaagccc
     4141 ggcatccgcc cgatggacag tgccggatcg ttacggcaga atcgcgcgcc ttccccgttc
     4201 cgggcgcccg agcgcgccgc cctccctggc gagagtccct tcccgatggc gcagctgctg
     4261 gtgctgaacg gccccaacct gaacctgctc ggcacccgcg aacccgcgac ctacggcgcg
     4321 cagacgctgg ccgacatccg ccagaactgc gaagccctcg ccgccgcgca ggggcatgcg
     4381 ctcgacttct tccagagcaa cgccgaacac gcgctgatcg agcgcatcca cgccgcgcgc
     4441 accgacggca ccgacttcat cgtcatcaat cccgccgcgt acacgcacac cagcgtggcg
     4501 ctgcgcgacg cgctgctggc cgtcgcgatt ccgttcatcg aggtccacct ctccaacgtc
     4561 cacgcccgcg aacccttccg ccaccattcg tatttctccg acgtggcgct cggcgtgatc
     4621 tgcggactgg gcgcccaggg ctacgaactc gccctggcag cggcggaccg tcacctcgca
     4681 gcccgcaagc cggcatagga catccatgga catccgcaag atcaagaagc tcatcgaact
     4741 gctggaggaa tccggcatcg ccgaaatcga gatcaaggaa ggcgaggagg ccgtgcgcat
     4801 ctcgcgccat ccggccgcga gcgccgccac gccggtgctg atgcaggcac
//
LOCUS       DAOQJH010000104         4845 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_10348_length_4845_cov_5.929019, whole genome shotgun sequence.
ACCESSION   DAOQJH010000104 DAOQJH010000000
VERSION     DAOQJH010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 4845)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4845)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4845
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10348_length_4845_cov_5.929019"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..890
                     /gene="trfA"
                     /locus_tag="PLQ18_03615"
     CDS             <1..890
                     /gene="trfA"
                     /locus_tag="PLQ18_03615"
                     /inference="COORDINATES: protein motif:HMM:NF018714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="plasmid replication initiator TrfA"
                     /protein_id="HNE00771.1"
                     /translation="RISATIKIYKQVATVVDPVQRINALQERAAVTKPPALPRWEDWQ
                     RSVPNEVVRSALFTVGNRRDRREFLVETELAAIGETQVTYQGVELRQDDEDVWLQALH
                     LAREQPLDAPIEFVPHAFLRAIGWPTTGHYYALLRDAFGRMQRTTVTFRNQRLHKGLS
                     ISLIRRFEWSDRDAGGSDDRCKVWIEPEMRLLFGNTQYTRIEWEQRRHLRPLAKWLHG
                     YFASHRTPLPVRVVTLRDACGSDTRELRNFRPKLKAALTELVEACFLSDWQIDETDLV
                     HVCRAPAPGVDACGQTRLI"
     gene            1145..2359
                     /locus_tag="PLQ18_03620"
     CDS             1145..2359
                     /locus_tag="PLQ18_03620"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNE00772.1"
                     /translation="MSKPTGPAPDYSLTTDRLRALGEVSSKLIHELRAYSTSPEQRKL
                     LQKQWRIGEVAKLLGISESSLRNHDEALRGGEGNTGGAHRRYSLSDIHRLRELTHRQA
                     HRDPLRDRCARLAVCNFKGGSGKSTTATNLATHLVLHGYRTLFIDMDPQGSATALLYP
                     DNLMDIQRENTLYEVLFTQPERIRDLVIQTAWQGLDMIPAALSLFSVEAEILRYGFSN
                     PDFLFWERLDRALGHLQDDYDVILFDTPPSLSQTTVIAAWSATALVIPVQASMMDLQA
                     MASFFFGMWQSLSAIEQITGETKHYDFGLIVISNYRGNPKTMQDEYDVATWMRKMYQH
                     YVAEDAILNSSVIRSAANSIQIFYELPRRSDPSYARALGAFEGLGDQIIGLIRRQWPS
                     TQTAATRTASAS"
     gene            2373..3410
                     /locus_tag="PLQ18_03625"
     CDS             2373..3410
                     /locus_tag="PLQ18_03625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HNE00773.1"
                     /translation="MTSKPTRRKPRFEGLTLPSILDATPAAPGTAPAPAVPVADYVRE
                     AGQRLTEDYRRLRQLEQSGRVELELPLASIRPSRFANRSEHYFHSARYAEIRSSIAQH
                     GVKIPIKVRPVADSTPPAYEIVYGHTRHRASLDLGLPSIRAVVEALDDETLLAEMELE
                     NRDREDLSAYERARFYALALADTFAGNRERLCARFGRSNAWLSQQRVIAGLPQRLFDA
                     MPLLYQAGVEPLLELARLRPTDEQVQTLIEMHPDASSATTLVQVLIRRLHSASRGSPK
                     RTTASAEAVHHHRNGRLLMHARPGTRGRVQFEFESGQRGFADFLRTRLAELYDEYEKS
                     QAGEKPRIKGL"
     gene            complement(3684..>4845)
                     /gene="eno"
                     /locus_tag="PLQ18_03630"
     CDS             complement(3684..>4845)
                     /gene="eno"
                     /locus_tag="PLQ18_03630"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007852662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HNE00774.1"
                     /translation="ELRDGDARRYLGRGVTRAVGHVNGEISRALGGRDAGDQTGIDRC
                     LIELDGTPDKSRLGANALLAVSLASARAAAAAADLPPYRCLNGAQTQFHLPVPMMNIV
                     NGGVHAANSIDLQEFMILPVGAASFAEALRWGTEVYHALKSRLARLGLATAVGDEGGF
                     APDLPSSEAALEQLMAAIEQAGFRPGTDIGLGLDLASTEFHHDGRYVLASEQRSFDAA
                     GFVDHLAYLADSYPILSIEDGMAEDDHEGWRLLTARLGDRVQLVGDDLFVTNTRLLRR
                     GIDAGLANAILIKPNQIGTLSETLAAIAMAEAAGYGAVISHRSGETEDTTIADLAVCT
                     TATQIKTGAPCRSDRVAKYNRLLLIEAELGAEARYRGRSAFCGMPRYPLAVD"
BASE COUNT          905 a         1670 c         1516 g          754 t
ORIGIN      
        1 ttagaatctc ggcaaccata aaaatctata aacaggtagc caccgtggtc gatccggttc
       61 aacgcatcaa cgcgctgcag gagcgcgctg cggtcacgaa acccccggcg ctgccgcgct
      121 gggaggactg gcagcgcagc gtgccgaacg aggtcgtgcg ctcggcgctg ttcacggtcg
      181 gcaaccggcg cgaccggcgc gagtttctgg tcgagaccga actggccgcc atcggcgaga
      241 cgcaagtcac ctaccagggc gtcgaactgc gccaggacga tgaggacgtc tggctgcagg
      301 cgctgcacct ggcgcgcgaa caaccgctcg atgcgccgat cgagttcgtc ccgcatgcct
      361 tcctgcgcgc gatcggctgg cccaccaccg ggcactacta cgcgctgctg cgcgatgcgt
      421 tcggccgcat gcagcgcacg acggtcacct tccgcaacca gcgcctgcac aagggcctgt
      481 cgatctcgct gatccggcgc ttcgagtgga gcgaccgcga cgccggtggc agtgacgatc
      541 gctgcaaggt ctggatcgag ccggagatgc ggctgctgtt cggcaacacg cagtacacgc
      601 gcatcgaatg ggaacagcgc cggcacctgc gaccgctcgc gaagtggctg catggctact
      661 tcgcgagcca tcgcacgccg ctgccggtgc gggtcgtgac gctgcgtgat gcctgcggtt
      721 cggacacccg cgagctgcgc aacttccgcc cgaagctcaa ggccgcgctg accgagctgg
      781 tcgaagcctg cttcctgagc gactggcaga tcgacgagac cgacctcgtg cacgtgtgcc
      841 gggcaccggc acccggcgtg gacgcctgtg gacaaacgcg cctcatctga cgaaccggca
      901 ttcgtaacat gaggcgcaga gattcgtaac atgaggcgca cccacacgta atatgaggcg
      961 caggcacgta acatgaggcg caggaggcac gtaacatgag gcgcaaaaca cacgtaacat
     1021 gaggcgcggc agctcaaaaa atgcgctcgc aagcaattga ataaaaaggc gaatttcgtt
     1081 tgtatcaaaa acactaacta ttcttttaac cattctttta accaaaagaa aaagagcgcc
     1141 tccgatgtcg aaaccgaccg gccccgcccc cgactactcg ctgacgaccg atcggctgcg
     1201 tgcgctcggc gaggtcagca gcaagctgat ccacgaactg cgcgcctact cgaccagccc
     1261 ggaacagcgc aagctgctgc agaagcagtg gcgcatcggg gaagtcgcca agctgctcgg
     1321 catcagtgaa tccagcctgc gcaaccacga cgaagccctg cgcggtggcg aaggcaacac
     1381 cggaggcgca caccggcgct actcactgag cgacatccac agactgcgcg aactgaccca
     1441 ccgccaggca caccgggacc cgctgcgcga tcgctgtgcc cgactcgccg tctgcaactt
     1501 caagggcggc agcggcaaga gcacgacggc aaccaacctc gccacgcatc tggtcctgca
     1561 cggttaccgc acgctgttca tcgacatgga cccgcagggc tcggcgaccg ccctgctgta
     1621 tccggacaac ctgatggaca tccagcgcga gaacaccctc tacgaggtgc tgttcacgca
     1681 gcccgaacgc atccgcgatc tcgtgatcca gaccgcctgg caaggactgg acatgattcc
     1741 ggccgccctg tcgctgttct cggtcgaagc cgagatcctg cgctacggct tcagcaaccc
     1801 ggacttcctg ttctgggaac gccttgatcg ggccctggga cacctgcagg acgactacga
     1861 cgtaatcctg ttcgacaccc cgccgtcgct gtcgcagacc accgtgatcg ccgcctggag
     1921 cgccacggcc ctggtgatcc ccgtgcaggc ctcgatgatg gacctgcagg cgatggcctc
     1981 gttcttcttc ggcatgtggc agtcgctgtc ggccatcgaa cagatcaccg gcgagaccaa
     2041 gcactacgac ttcggactga tcgtgatcag caactaccgc ggcaatccga agacgatgca
     2101 ggacgagtac gacgtcgcca cctggatgcg aaaaatgtat cagcactacg ttgccgagga
     2161 cgcgatcctg aactccagcg tcatccgttc agccgccaac agcatccaga tcttctacga
     2221 gctgccgagg cgctcggatc cctcctatgc ccgcgcactc ggcgccttcg aaggtcttgg
     2281 cgaccagatc atcggcctga tccgccggca gtggccatcg acgcagacgg cagccacgcg
     2341 cacggccagc gcttcctgac ccggaacccg tcatgaccag caagccgacc cgcaggaaac
     2401 cgcgcttcga aggcctgacc ctgccaagca tcctcgacgc gactccagcc gcgccgggaa
     2461 ctgcaccggc cccggccgtg ccggttgccg actacgtgcg cgaggccggc cagcggctga
     2521 ccgaggacta ccgtcgcctg cgccagctcg aacagagcgg acgagtcgaa ctcgaactgc
     2581 cgctggcgag catccggcca tcgcgcttcg ccaaccgcag cgagcactac ttccacagcg
     2641 cgcgctatgc cgaaatccgc agcagcatcg cgcagcacgg cgtgaagatt ccgatcaagg
     2701 tccggccggt cgctgacagc acaccgccgg cctacgaaat cgtttacggc cacacgcgcc
     2761 atcgcgccag cctcgatctc ggcctgccga gcatccgtgc cgtcgtcgaa gcgctcgacg
     2821 acgaaaccct gctcgccgag atggaactcg agaaccgcga ccgcgaggat ctctctgcct
     2881 acgagcgtgc ccgcttctac gccctcgccc ttgccgacac ctttgccggc aaccgcgaac
     2941 ggctgtgtgc ccgcttcggg cgcagcaatg cctggctgtc gcagcagcgc gtgatcgccg
     3001 gcctgccgca gcgtctgttc gacgcgatgc cgctgctgta ccaggccggt gtcgagccgc
     3061 tgcttgagct cgcacggctg cgcccgaccg atgaacaggt gcagacgctg atcgagatgc
     3121 acccggatgc gtccagtgcc accaccctcg tgcaggtcct gatacgccgg ctgcactccg
     3181 caagccgcgg cagcccgaag cgcacgacgg ccagcgctga agccgtgcat caccatcgca
     3241 acggccgcct gctgatgcac gcacggcccg gcacacgtgg tcgcgtgcag ttcgagttcg
     3301 aatccggcca gcgtggtttc gccgatttcc tgcgcacccg gctcgctgag ctgtatgacg
     3361 agtacgaaaa atcgcaagcg ggtgaaaaac caaggataaa aggcctttaa cgcctgccgt
     3421 cgccgccgca ggcacgagtt ccggcaagct tccggacagc ttctccggcc cggtccggac
     3481 caggttttga atcattaccc atatgattct gaaagaataa aaatcgtcgg cgagcgggtg
     3541 aatgatcgtc cggactcgta agatgaggcg cagtgccggc ctgcccgccc ctgcgcccgc
     3601 tgccggcacc gcaggcatgc tccgatgcct gcggcccgct accgggaatg ctgcgcacgg
     3661 ctcgcagccg cagcgtcagc ggctcagtcg acggccagcg gataccgggg catgccgcag
     3721 aacgcgctgc ggccccggta acgcgcctca gcaccgagtt cggcctcgat cagcagcagg
     3781 cggttgtatt tggccacgcg gtcgctgcga cacggcgcgc cggtcttgat ctgcgtggcg
     3841 gtggtgcaga ctgcgagatc ggcgatcgtc gtgtcctcgg tttcgccgga gcgatgcgag
     3901 atcacggcgc cgtagccggc cgcctcggcc atcgcgatgg cggccagggt ttcgctcagc
     3961 gtgccgatct ggttcggctt gatcaggatg gcgttggcaa ggcctgcgtc gatgccacgg
     4021 cgcagcagcc gtgtgttggt cacgaacaga tcatcaccga ccagctgcac gcgatcgccg
     4081 aggcgcgcgg tcagcaatcg ccagccctcg tggtcgtcct cggccatgcc gtcctcgatg
     4141 ctgaggatcg gatagctgtc ggccagatag gccagatgat cgacgaagcc ggccgcatcg
     4201 aacgagcgct gctccgaggc gagcacgtag cgtccgtcgt ggtggaactc ggtgctcgcg
     4261 aggtcaagac caaggccgat gtcggtaccg ggccggaagc cggcctgttc gatcgcggcc
     4321 atcagctgct ccagcgcggc ctcgctcgaa ggcaggtccg gcgcgaagcc gccctcgtcg
     4381 ccgaccgccg tcgccaggcc gaggcgcgca agccggctct tcagcgcgtg gtacacctcc
     4441 gtgccccagc gcagcgcctc ggcgaagctc gcggctccga ccggcaggat catgaattcc
     4501 tgcaggtcga tgctgttggc ggcatgcacg ccgccgttga cgatgttcat catcggcacc
     4561 ggcagatgga actgcgtctg tgcgccgttc aggcagcgat acggtggcag atccgcagcg
     4621 gcggccgcgg cgcgggcgct cgccagcgac acggccagca gcgcgttggc accgaggcgt
     4681 gacttgtcgg gcgtgccgtc gagctcgatc aggcagcggt cgatgccggt ctggtcgccg
     4741 gcatcgcggc cgcccagggc gcggctgatc tcgccgttga cgtgaccgac cgcgcgggtc
     4801 actccgcggc cgaggtagcg gcgggcatcg ccgtcgcgca gctcc
//
LOCUS       DAOQJH010000105         4814 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_10433_length_4814_cov_3.444841, whole genome shotgun sequence.
ACCESSION   DAOQJH010000105 DAOQJH010000000
VERSION     DAOQJH010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 4814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4814
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10433_length_4814_cov_3.444841"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="PLQ18_03635"
     CDS             complement(<1..230)
                     /locus_tag="PLQ18_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HNE00775.1"
                     /translation="MELIGHLQLGFAAAAGAQNLLACFTGALLGTLIGVLPGLGPVAT
                     IALLLPLTYGLPPLTALIMLAGIYYGAQYGGST"
     gene            complement(221..676)
                     /locus_tag="PLQ18_03640"
     CDS             complement(221..676)
                     /locus_tag="PLQ18_03640"
                     /inference="COORDINATES: protein motif:HMM:NF018979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HNE00776.1"
                     /translation="MFHDPRDGLVGLALAATGAGALWIARRYPAGTAADLGPGFLPGI
                     LGALLLLLGLLMAAGACRGPRTPFPVLQLRPLLAVLGGVVLFGLLLRSAGLLVASSAL
                     VLLASAGLRGAGWRVALIEAVVLGVLAVAVFVHGLGLRLPVWPAGLPWN"
     gene            complement(712..1689)
                     /locus_tag="PLQ18_03645"
     CDS             complement(712..1689)
                     /locus_tag="PLQ18_03645"
                     /inference="COORDINATES: protein motif:HMM:NF015366.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HNE00777.1"
                     /translation="MFRRLTALSMMLMMLTTLVPVPAGAQAGEYPQRPVTLIVTLPAG
                     GPTDAAARTLATALSQALGQPFVVENRPGADGAIGARAVVEAAPDGYTLLFGVGSLVA
                     LPHLQSPPPFDALRDLAPVAAIGSYPFALFVHPSVPAANVAEFIEYARSRPEPLFYSA
                     STPGEALAATAFMQATGVPLTRVPYKGSSQALPDLLAGRVQLTFGPVATGLPHVREGL
                     LNALAVLTPKRLPALPDTATLEEAGIRGVTVPSWQAMLAPAGTPPAILDQLASATAAA
                     LETAPVRERLTKLLLQIELLPPAALAQRIEADDALWQRHFAGTAAARQP"
     gene            1875..2900
                     /locus_tag="PLQ18_03650"
     CDS             1875..2900
                     /locus_tag="PLQ18_03650"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNE00778.1"
                     /translation="MDKLRAIQYFVAAVREGSLSAAARRLEVSVPAVAKLVAALEREL
                     GASLLDRSPRGLALTADGERYLAVCEGVLQQLAGIERQLGRQGAGPRGSVVVELPPLL
                     ARFWILPALPELHRQYPEVQIELRLIDRSTITDADAGGCDVCIVLGWPEALDRVVRQV
                     ARMRLLVCATPAYWARHGQPQRPRDLARHVACLVRNPKGVLLDYWRYRRGEEEEAVKL
                     SGWLVSDHRDAVLDAVLAGEGIGRFADLSVQHLLRTGELVPVLLDWETPDDPPVNLLH
                     RPLAQGPSALRVVVEFLDARIRQLEAGRGAPHADAEPDARPLWYRRAYGRASAVGGAG
                     GSDAERM"
     gene            3315..3617
                     /locus_tag="PLQ18_03655"
     CDS             3315..3617
                     /locus_tag="PLQ18_03655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE00779.1"
                     /translation="MQTFAVDGLNLDGGISASQGRTGRTGAGSQGSDGTPSGIDADTW
                     GIHGDAPAIHGNPQGIHGDASGIAPGRSGIGADVAGIAAGTDGSLAGGRTMRLQPS"
     gene            complement(3612..4070)
                     /locus_tag="PLQ18_03660"
     CDS             complement(3612..4070)
                     /locus_tag="PLQ18_03660"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HNE00780.1"
                     /translation="MRISIREATVADIPAVAGLFDLYRQFYEQPPDLPLAKEFIAKRI
                     ANRESAILVAESTADGLLGFCQLYPSFCSVEAQPIYVLYDLFVAPAARKAGVGRMLLC
                     AAEATAVTNGKARMDLTTARTNTRAQALYESLGWVRDDIFFAYNRRIPRS"
BASE COUNT          764 a         1604 c         1707 g          739 t
ORIGIN      
        1 gtcgagccgc cgtactgcgc gccgtaatag atgccggcca gcatgatcag cgccgtcagc
       61 ggcggcaggc cgtaggtcag cggcagcagc agcgcgatgg tcgcgaccgg gccgaggccc
      121 ggcagcacgc cgatcagcgt gccgagcaaa gcaccggtga agcaggcgag caggttctgc
      181 gccccggcgg cggcggcgaa gccgagctgc aggtgcccga tcagttccac ggcagccccg
      241 caggccagac cggcagccgc aggccgaggc cgtgcacgaa cacggcgacg gccagcaccc
      301 cgagcacgac ggcctcgatc agtgccaccc gccagccggc gccgcgcagg ccggcgctcg
      361 cgagcagcac cagcgcgctg ctcgccacca gcagcccggc actgcgcagc agcagcccga
      421 acagcacgac gccgccgagc acggcgagca gcggccgcag ctgcagcacc gggaacggag
      481 tgcgcgggcc gcggcaggcg ccggcggcca tcagcaggcc gagcagcagc agcaaggccc
      541 cgagtatgcc cggcaggaag ccgggtccga ggtcggcggc cgtgccggcc ggatagcgcc
      601 gggcgatcca cagcgccccg gcgccggtgg cggcgagggc cagtccgacc aatccgtcgc
      661 gagggtcatg aaacacgggg gaggggctcc gcgggccgcc ggggtgtttg ctcacggctg
      721 gcgggccgcg gcggtgccgg cgaaatgacg ctgccagagc gcgtcatcgg cctcgatgcg
      781 ctgcgccagc gccgccggcg gcagcagctc gatctgcagc agcagcttcg tcaggcgctc
      841 gcgcaccggg gcggtttcca gcgccgcggc agtcgccgag gccagctgat cgaggatcgc
      901 cggcggcgtg ccggccgggg ccagcatcgc ctgccacgaa ggcaccgtca cgccccggat
      961 gccggcttcc tccagcgtcg cggtgtcggg cagcgccggc aggcgcttcg gcgtcagcac
     1021 cgcgagcgcg ttcagcagcc cctcgcgcac gtgtggcagg ccggtggcca ccgggccgaa
     1081 ggtcagctgc acgcggccgg ccagcaggtc gggcagggct tgcgacgagc ccttgtacgg
     1141 cacgcgcgtc agcggcacgc cggtggcctg catgaaggcg gtcgcggcca gcgcctcgcc
     1201 gggcgtgctc gcggagtaga acagcggttc gggacggctg cgcgcgtatt cgatgaattc
     1261 cgcgacgttc gccgccggca ccgagggatg cacgaacagc gcgaacggat agctgccgat
     1321 cgcggcgacc ggcgccagat cgcgcagggc atcgaagggc ggcggcgact gcaggtgcgg
     1381 cagcgcgacc agcgagccga cgccgaacag cagcgtgtag ccgtcggggg ccgcctcgac
     1441 gaccgcgcgc gcgccgatcg cgccatcggc gcccgggcgg ttctcgacga cgaagggctg
     1501 gccgagcgcc tgcgacagcg ccgtcgccag cgtgcgcgcc gcggcatcgg tcggtccgcc
     1561 ggccggcagc gtcacgatca gcgtcaccgg ccgctgcggg tactcgcccg cctgcgcgcc
     1621 ggcgggcacc ggcacgagcg tcgtcaacat catcagcatc atcgacaagg ctgtcagcct
     1681 gcggaacatg gcgcctcccg tggaatggtc gtcagggcgg gcaccggccg ggccggcggc
     1741 ggaccggcac tgcggcgagc gccgtcgaca caggctaggc agcgaccggc caggtccgtg
     1801 agagccgctt tggcatgcat ggccttaacc gctggtttag gctgagcccc ggtttccggc
     1861 cggaaagatg cttcgtggac aagctgcgcg ccatccagta tttcgtcgcc gccgtgcgcg
     1921 agggcagcct gagtgctgct gcgcgccggc ttgaagtcag cgtgccggcg gtcgccaagc
     1981 tggtcgcggc gctggagcgc gagcttggtg cgagcctgct ggatcgcagt ccgcgcggac
     2041 tggcgctgac cgccgacggc gagcgctatc tcgcggtctg cgaaggcgtg ctgcagcaac
     2101 tcgccggcat cgagcgccag ctcggccgcc agggggcagg tccccgcggc agcgtcgtcg
     2161 tcgaactgcc gccgctgctc gcacgcttct ggatcctgcc ggcgctgccc gaactgcacc
     2221 ggcagtaccc ggaggtgcag atcgagctgc gcctgatcga ccgctcgacg atcaccgatg
     2281 ccgatgccgg cggctgcgat gtctgcatcg tgctcggctg gccggaggcg ctcgatcgcg
     2341 tcgtgcgcca ggtcgcgcgc atgcgcctgc tggtgtgcgc gacgcctgcc tactgggcgc
     2401 ggcacggcca gccgcagcgc ccgcgcgatc tggcgcgcca cgtcgcctgt ctggtgcgca
     2461 atccgaaggg cgtgctgctc gattactggc gctaccgccg gggcgaggag gaggaggccg
     2521 tgaagctctc cggctggctg gtcagcgatc accgcgacgc cgtgctcgat gccgtgctcg
     2581 ccggcgaggg catcgggcgt ttcgccgacc tgtcggtcca gcacctgctg cgcaccgggg
     2641 aactcgtgcc ggtgctgctc gactgggaga cgcccgacga tccgccggtc aacctgctgc
     2701 accggccgct ggcgcagggc ccgagcgcgc tgcgcgtcgt cgtcgaattc ctcgatgccc
     2761 gcatccgcca gctcgaagcc ggccgcggcg ctccgcatgc ggacgcggaa cccgacgccc
     2821 ggccgctctg gtaccgccgc gcctacgggc gggcgtcagc ggtcggcggg gcggggggca
     2881 gtgatgccga gcgcatgtga tggtccggcc atgttgattc gttgaatcgc gcgtgtgccg
     2941 gtcgctccgg gcgcgaccgg gtcgcacggc cgggcgcgac gatcgggatc gcgccagcgc
     3001 tcgcccgcac ccgcttgccc cttgccgaag tcccggcccc gccccgcgcc ggggcgccgt
     3061 tgccccgaaa agcgtcggcg tcgatacctg aactgccggc gtcgatgccc cggcatcgct
     3121 gccggtggtt cacaccccgg cgccggcctc ctgaacgccg gcttcgactc ctgaagtgtc
     3181 ggcggcggca cttcgaacac ccccgggact gcgcaggtat cggcgtcgat acctgaaacc
     3241 ccgacgccgc tgttacgtgg ggcggcggcg ccccagaaag gggcggccgg gggctttgga
     3301 accccgaacg ggatgtgcag acattcgccg tcgatgggct gaacctcgac ggcgggattt
     3361 cagccagcca gggcaggact ggcaggaccg gcgccggtag tcagggtagt gacggcactc
     3421 cctcgggtat cgacgccgat acctggggca tccacggcga tgctccggct atccacggca
     3481 acccccaggg tatccacggc gacgcttccg gaatcgctcc cggccgttca ggcatcggcg
     3541 ctgatgttgc gggtatcgct gccggtacgg acggcagcct tgcaggcggc cgcaccatgc
     3601 ggctgcagcc ctcatgaccg ggggatgcgg cggttgtagg cgaagaaaat gtcatcgcgc
     3661 acccatccaa gagactcgta aagcgcctgg gcgcgcgtgt tggtccgtgc ggtggtgagg
     3721 tccatgcgcg ctttgccgtt ggtaacggcg gttgcctcgg ctgcgcacaa cagcatgcga
     3781 ccgacaccgg ccttgcgggc ggcaggcgcg acaaagagat cgtaaagcac atagatgggc
     3841 tgtgcctcga ccgaacagaa gctcgggtag agctggcaga agccgagcag cccgtcggct
     3901 gtggactcag ccacgagaat ggccgattcc cggttggcga tgcgtttggc gatgaactcc
     3961 tttgcaagcg gcaggtccgg cggctgttca tagaactgtc gatacaggtc gaacagcccg
     4021 gcgacggccg ggatgtcggc gacagtggct tccctgatgg aaatgcgcat aatctgggtt
     4081 ttccgtgaac ctgcgttgga aatcagtcga ttgccaaaag ccatcggaga cggatgttga
     4141 cggtcgtccg gatcaccggc tcaggctgcc cgacgccagt cggtcgtaag cgattgttat
     4201 atttcgttct tttagggtgc gcagctttgc tgcgcaccct gcggcgcatg aacctgaacc
     4261 cggcactgag ggtgcgcaac gagctgcact tgtgcgggtc ttgattatta atcgtcgcct
     4321 atatttagtt caatcaagcc gtcgtcgatt tctgtttggg aaaaacaaaa ctttaaaatg
     4381 ccgcctagtg ttccttcgta atatattctt gccttgggga ctggttcgcg ctttccatct
     4441 ggattgattt ctatttcgcc atgaaaaaca catcctgctc taagtggtag tgcgccattg
     4501 ctgatttgtg tatcgaactc actgtcgcct tcaaagcaga tgaaaggtag atctgacgat
     4561 tggtgttgtc gggggatgca tgaagatttt gttgtccaaa aaagtgtcat atgatttgca
     4621 ggtccgtaat gaatgaggcg ccctcatcgt ggcgcctcag gttccggcgg catgcgcatg
     4681 ttaaaagcga agaacgtaac ggccgtcctg gaatgctatt taagcgcctg cgccgtcaga
     4741 tccagacact tccacgcctt ctcgaccagt tcgtcgatgc cgtcgcgatc gaggatcagc
     4801 ggcggggcga cgat
//
LOCUS       DAOQJH010000106         4769 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_10591_length_4769_cov_3.403479, whole genome shotgun sequence.
ACCESSION   DAOQJH010000106 DAOQJH010000000
VERSION     DAOQJH010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 4769)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4769)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4769
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10591_length_4769_cov_3.403479"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            115..3618
                     /locus_tag="PLQ18_03665"
     CDS             115..3618
                     /locus_tag="PLQ18_03665"
                     /inference="COORDINATES: protein motif:HMM:NF019313.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase fold protein"
                     /protein_id="HNE00781.1"
                     /translation="MEEFSGGIRRALAEAAGLALTPVAEHTACFAAWLNALRDDDYHV
                     SFSSLMLGVRLLGYDDWFEQQCEAALGPPPRMLATLFGGLLGERTLDTARACAASGHW
                     PDEDWPNAAEPAPVAGVPWPRRSGRGELSASAASLLRFAGNRARTRQATALDAIDLIA
                     AFVLDTHGHERQMESQGFELQRLRAAFAAREDEAALHGTGSGSGAPSSVAGAGAGADH
                     PLCRLRCDDETRYVLRHAVNLALGARRHAPPILTLRQILYALLEAGSGDGPVVTARGL
                     RASLDDFYRPGLGDEVAATGTQPDWPPPPFAAGEPLPEVWVEVTGVLDRAVRWCAGDP
                     LELPALALALVAEASDAATRAFAPLKPLTQAGIGERWLAALHAGAVTAGRDPSRYEQW
                     LYGEHRAAPSGVLPDLGRPRDAGDDCLDVRRYALALAATAVARDTAPPLAIGVFGDWG
                     AGKSHFMGLMELEMRTLAAGGDARFHRHVLPVWFNAWHYADANLWASLMQALLEAVAG
                     HFTAGTEPGEDADFTRLLRRLDAASSGAALARTELAAAERSADEARKQFETARDQAQI
                     QECAVQARLADLFQELLAGQSGASIGKDLADELTALGFDELAKTAAAAGNRYDAILAT
                     VAQAETLTADARAFWLALRQWQWIDAGLLLAIAAGGGALIWALHAEATGQMLGAALTA
                     SGAAAAALGWLRQRFGALGRLRERLAGIRQKQAEAAARAALSEPARAAETARRDLEAA
                     RQRLDAALAARQQATAAVSESFVSARIARFVEQRLKSGLYREQLSLVSTIRRDLDTIA
                     RFLGQQSRERAQQEAQARERLAGLGLGHKATPALDRIVLYIDDLDRCPPERVVEVLEA
                     VHLLLAMPLFVVVVGVDVRWVSAALCRRYRGLLVGERGAAAGAHPTATPTDYLEKIFQ
                     IPFRLPAIDAVPARRLLGTLTGVSLLASGLRIPGPAVRVRPDASDAASRAAVPAGPDT
                     PAVVTERPHEPADAGAGAAGADAPQAAEAQHEAGAADEARHTMIAVALSFSADEAGFM
                     ARLLATLGGSPRRVRRFVNSYRVYKAALRDAELRALTEGGGYREVLTALALQIGLNPH
                     LGRLREALLHRPGDDGQVQAQAPARVPEIGLDPAHRARLENALDALRADGDTPERERR
                     RLLDLLDRPDIARFSFAPAQR"
     gene            4299..>4769
                     /locus_tag="PLQ18_03670"
     CDS             4299..>4769
                     /locus_tag="PLQ18_03670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE00782.1"
                     /translation="MSQIVSAVYATHADAEAARQALIVDGFEAAEVHITSLATGDDTT
                     PSAQGGGVRGFFRSLFGLEPEHPEVERYVAAVDSGRTVVSIEVDDDEALAARTVLLRY
                     GPVDLPPGEGYVTDADRLRNGDAESEYRTTMPFGESICAGAAGAAGEPGARPGTT"
BASE COUNT          616 a         1775 c         1735 g          643 t
ORIGIN      
        1 cgtgccgtct cgtaggatgg gctgacgcag gaagcccatc tttaccgccc ccgccggatc
       61 cgccgcgccg ctcctacact cggccatcgc ccataagcga ccgcggaggc agcgatggag
      121 gagttcagtg gtggcatccg ccgggcgctc gccgaggccg ccgggctggc gctgacgccg
      181 gtcgccgaac atacggcctg cttcgccgcc tggctgaacg cgctgcgcga cgacgactat
      241 cacgtctcgt tcagcagcct gatgctcggc gtgcgcctgc tcggctacga cgactggttc
      301 gagcagcagt gcgaagccgc cctcggcccg ccgccgcgca tgctggcgac gctgttcggg
      361 ggcctgctcg gcgagcgcac gctcgatacc gcgcgtgcct gcgcggccag cggtcactgg
      421 ccggacgagg actggccgaa cgccgccgag ccggcgccgg tcgccggcgt gccgtggccg
      481 cggcgcagcg gccgtggcga gctgagcgcc tcggcggcga gcctgctgcg cttcgccggc
      541 aaccgcgccc gcacgcgtca ggccacggcg ctcgatgcca tcgacctgat cgctgccttc
      601 gtgctcgaca cccacggcca cgaacggcag atggagagcc agggcttcga gctgcagcgg
      661 ctgcgcgccg ccttcgcggc gcgtgaggac gaagcggcgc tgcacggcac cggcagcggc
      721 agcggggcgc cttcgtctgt cgccggcgcc ggcgccggcg ctgaccatcc gctgtgccgg
      781 ctgcgctgcg acgacgaaac ccgctacgtg ctgcgccacg cggtgaatct cgcgctcggc
      841 gcccgccggc acgcaccgcc gatcctgacg ctgcgccaga tcctgtacgc gctgctcgaa
      901 gccggcagcg gcgacgggcc ggtggtgacg gcacggggtc tgcgcgccag tctcgatgac
      961 ttctatcgcc ccggcctcgg cgacgaggtc gccgccaccg gcacgcagcc ggactggccg
     1021 ccgccgccgt tcgcggccgg cgagccgctg cccgaggtct gggtcgaggt cacgggcgtg
     1081 ctcgatcgcg ccgtgcgctg gtgcgctggc gatccgctcg aactgccggc gctggcactg
     1141 gcgctggtcg cggaagcctc cgatgcggcc acccgcgcct tcgcgccgct caaaccgctg
     1201 acgcaggccg gcatcggcga gcgctggctg gcggcgctgc atgccggggc ggtcacggcc
     1261 ggtcgcgatc cgtcgcgcta cgagcagtgg ctgtacggcg agcaccgcgc cgcgccctcc
     1321 ggcgtgctgc ccgatctcgg ccgcccgcgc gatgccggcg acgactgcct cgacgtgcgc
     1381 cgctacgcgc tggcgctggc ggcgaccgcg gtggcgcgcg acaccgcacc gccgctggcg
     1441 atcggcgtgt tcggcgactg gggcgccggc aagagccact tcatggggct gatggagctg
     1501 gagatgcgca cgctcgctgc cggcggcgat gcgcgcttcc accgtcatgt gctgccggtg
     1561 tggttcaacg cctggcatta cgccgatgcc aatctgtggg cgagcctgat gcaggcgctg
     1621 ctggaggcgg tcgccgggca tttcacggcg ggcacggagc cgggcgagga cgccgatttc
     1681 acccggctgc tgcgccggct cgatgccgcc agcagcgggg cggcgctggc ccgcaccgaa
     1741 ctcgccgcgg ccgagcgcag cgccgatgag gccagaaagc agttcgagac ggcccgcgat
     1801 caggcgcaga ttcaggaatg cgccgtgcag gcccggctgg ccgacctgtt tcaggaactg
     1861 ctcgccggcc agagcggtgc cagcatcggc aaggacctcg ccgatgagct gacggcgctc
     1921 gggttcgacg aactggcgaa gacggccgcg gccgccggca atcgctatga cgcgatcctc
     1981 gccacggtgg cgcaggccga aaccctgacc gccgatgccc gcgcgttctg gctggcgctg
     2041 cgccagtggc aatggatcga tgccggcctg ttgctggcga tcgcagccgg cggcggcgcg
     2101 ctgatctggg cactgcatgc cgaggccacg gggcaaatgc tcggtgccgc gctgacggcg
     2161 agcggtgcgg cggcggccgc gctcggctgg ctgcgccagc gcttcggtgc gctgggccga
     2221 ttgcgcgaac gtctggccgg catccggcag aagcaggccg aagccgctgc cagggccgcg
     2281 ctcagcgagc cggcgagggc ggccgaaacc gcccggcgcg atctggaagc cgcgcgccag
     2341 cgcctcgatg cagccctcgc cgcccgccag caggccacgg ctgccgtgtc ggaatccttt
     2401 gtttcggccc gcatcgcccg cttcgtcgag cagcggctga agtccgggct gtatcgcgag
     2461 cagctcagtc tggtctcgac gatccgccgc gacctcgaca ccatcgcgcg cttcctcggc
     2521 cagcagtccc gggagcgggc gcagcaggag gcgcaggcgc gcgaacgtct ggcaggtctg
     2581 ggtcttggcc acaaggccac gccggcgctc gatcgcatcg tgctctacat cgacgacctc
     2641 gaccgctgcc cgcccgagcg cgtcgtcgag gtgctcgaag ccgtgcatct gctgctggcg
     2701 atgccgctgt tcgtggtcgt ggtcggcgtc gatgtgcgct gggtatcggc ggcgctgtgc
     2761 cggcgctacc gcggcctgct ggtcggcgag cgcggcgctg cggcgggcgc gcacccgacc
     2821 gcgacgccga ccgattacct cgaaaagatc ttccagatcc cgttccggct gccggccatc
     2881 gacgccgtgc cggcccgccg cctgctcggc acgctgaccg gcgtgtcgct gctcgccagc
     2941 ggcctgcgca tccccggccc ggccgtgcgc gtgcggccgg atgcgagcga tgcagcgtcc
     3001 cgtgctgcgg tcccggccgg tcccgacacg ccggcggtcg tcacggagcg tccgcacgag
     3061 ccggcggatg cgggcgccgg ggcggccggg gccgacgccc cgcaggcagc ggaggcgcag
     3121 cacgaggccg gggccgcgga cgaggcgcgt cacacgatga tcgccgtcgc gctgagcttc
     3181 agtgccgacg aagccggctt catggcccgg ctgctggcca cgctcggcgg ctcgccgcgg
     3241 cgcgtgcgcc gcttcgtcaa cagctaccgc gtctacaagg cggcgctgcg cgatgccgag
     3301 ctgcgcgcgc tgaccgaggg cggcggctat cgcgaggtgc tgacggcgct ggcgctgcag
     3361 atcggcctca acccgcatct cggccgcctg cgcgaagccc tgctgcaccg gcccggggac
     3421 gacgggcagg ttcaggctca ggcgccggcg cgcgtgccgg agatcgggct ggaccctgcc
     3481 caccgcgccc ggctcgaaaa cgccctcgac gcgctgagag ccgacggcga cacgcccgag
     3541 cgcgaacgcc ggcgcctgct cgacctgctc gatcgccccg acatcgcccg cttcagcttc
     3601 gcaccggcgc agcgctgata ccgatcaccg cttcgctttt cagcccatcg ccccgcgtgg
     3661 gcatgggtat ctacacaaac cggcaattcc cgcgggcaac gggtcccggc accaaggctt
     3721 cggtctcccc tctccccttg cgggagaggg ggcgggggag agggggacgc tggcagcagc
     3781 ccagccgctg gccccctctc cccaacccct cccccgcgag ggggggaggg gctgaaaagc
     3841 aaagcgctct ggtgtgatcg gtatgaatcg cgctgaagca cgctgatccg cgccggccgg
     3901 ccgtggccgg caagccgttg aaccttctgc ccggcgcagc gttcggattg cgcatgacca
     3961 cacatccggc aggaatcgcg gtcgtcatgg cctgcacgcg ccgagcgcgt gctccggccc
     4021 gcagcgtccg cccgcctgcc cttgtccacg gtctgccggg gccgatgacc ggacccgtcg
     4081 cctgtgctgc cgcctcgatg cggcggcgca ccgtcagcga agcgccgcgc acgctgcccg
     4141 tcccggggcc ggccggaacg cgcagcggca ttcgcgtccg cagggtcacg gccgagggcc
     4201 accgacccgc caccatgact gccgcgcacc cgttgccgtg gcccttcgcc acgatccgtc
     4261 agccggggca gccgcagtcg aaggagtgag gattcgtcat gagccagatc gtttctgccg
     4321 tgtatgccac ccacgccgat gccgaggccg cgcgtcaggc gctgatcgtc gacggcttcg
     4381 aggccgccga ggtccacatc acctcgctcg ccaccggcga cgacaccacc ccgtccgcac
     4441 aaggcggcgg cgtgcggggt ttcttccgtt cgctgttcgg actcgaaccc gagcatccgg
     4501 aggtcgagcg ctacgtcgcg gccgtcgaca gcggccgtac cgtcgtcagc atcgaggtcg
     4561 atgacgacga ggcgctcgcg gcgcgcacgg tgctgctgcg ctacgggccg gtcgacctgc
     4621 cgcccggcga gggctacgtg accgatgccg accggctgcg caacggcgat gccgagtccg
     4681 agtaccgcac gacgatgccg ttcggcgaga gcatctgcgc cggcgcggcc ggggcggccg
     4741 gcgagccggg cgcgcgcccg ggcacgacg
//
LOCUS       DAOQJH010000107         4749 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_10652_length_4749_cov_4.455262, whole genome shotgun sequence.
ACCESSION   DAOQJH010000107 DAOQJH010000000
VERSION     DAOQJH010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 4749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4749
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10652_length_4749_cov_4.455262"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(46..819)
                     /locus_tag="PLQ18_03675"
     CDS             complement(46..819)
                     /locus_tag="PLQ18_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HNE00783.1"
                     /translation="MRATVLRLPSLLLLCAATLAMPAARAQEVDLEALAMLEAADQVR
                     FPRSDFEVNVRISSTTPGGETEVRDYQILSKGNENTLVITTAPASERGQVLLMRGRDL
                     WVYSPRLSQPVRLPLSQRLTGQVANGDLARANFSGDYNARVLRDETIQKRKYAVLELT
                     AIDGSVTYSRVLLWVERSGNQPLKAEFYALSGRLLKTAQYLNYAKLDNTVRPTQLVME
                     DAVKRGDRSVLDYSAMKPRELPNKLFNKDYMKRVTQGID"
     gene            914..1600
                     /locus_tag="PLQ18_03680"
     CDS             914..1600
                     /locus_tag="PLQ18_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNE00784.1"
                     /translation="MSIVRIENVSKRYRLGEQDVTALHGVSLRVEPGEFVALAGPSGS
                     GKTTLLNIIGCIDTPSSGRVIVDGHDVTGRDPDQLAELRARTLGFIFQTFNLLPVLSA
                     EENVEYPLINIQHIPRAERRQRVAYFLDMVGLARFARHRPNQLSGGQRQRVAIARALA
                     TKPKIVLADEPTANLDHQSGDGILQLMKHINEQSGTTFIFSTHDARVMAMASRLVRIS
                     DGAIVGEETR"
     gene            1597..2898
                     /locus_tag="PLQ18_03685"
     CDS             1597..2898
                     /locus_tag="PLQ18_03685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE00785.1"
                     /translation="MTNHPNRSGRIALGIALLAVTPAAAAQQDVKLSGYLSNETAYTF
                     GGENHWSKSRNTLNLAATGSFSESISWKASGWLRWDPVVQHSDFYPDKVQDDRGFEGL
                     VRETFLDVSLGDWDLRLGRQNIVWGEMVGLFFADVVSARDMREFVLADFEQLRIPQWA
                     ARAEYFKDDFHAELVWIPYLTYDNIGEPGDDFYPYPPAFPGLGYRILDEKKPRGGDSD
                     DAAGVRLGWIVDGWDINGYYYRSTDVQPAFQRSFGLVGGVPTLTYTPTHNRIQQWGLT
                     VGKDIGDGLVFKAEAIYTMGRRYDVLRLSDADGLVRKDTLDYVLGLDWTPDASNRLNL
                     QFFQRFYQDHDPDMVPESVESGVSLFYSTKVWGDRLEPEITLIESLNRGDWLLRPRLT
                     WNVSADLKASFGADILNGPPDALFGRFTNGDRVYAEVRYDF"
     gene            complement(2886..>4749)
                     /locus_tag="PLQ18_03690"
     CDS             complement(2886..>4749)
                     /locus_tag="PLQ18_03690"
                     /inference="COORDINATES: protein motif:HMM:NF006077.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HNE00786.1"
                     /translation="GAFHLADFDRFELHNFNRQAGATMATLDRPKLDVMAEAALAINP
                     ELDIRRFADGVTDDNLDAFFDGVDLYVDALDFFVLPVRRKVFAAAHARGIPAITAAPL
                     GMGTAVLTFLPGRMSFEDYFQVEGQPEEEQYARFLVGLSPTMLQQGYLVDRSTINFVE
                     KRGPSTPMACELAAGVLVTESLKLLLGRGPVRAAPWGLHFDAYRNRLSRTWRPGGNRH
                     PLNRLTLAIMRTLLRRARSLPAPARAAPAAPAGLDPRVARVLELASWAPSGDNTQPWR
                     FEAVDPLTVIVHGCDTRDHVIYDLRGRPSQLALGALLETARIAAPGAGLALDIETLPG
                     VGDTTPAFAPMFRLQFTPAAAAVRHPLETAIRARCTQRRPLSRRPLTDAVKAAFLQAL
                     GPGYSLRWLEGDQRLDVAKLLFRNAHIRLTTPEAYPTHRDIIEFGVQYSEDRLPGAAV
                     GLDPVGLALMRWAMKSWERVDFLNRWFAGTWLPRLQLDFAPALGCAAHFLIIAEQAPQ
                     TLADYLAAGAAMQRFWLTATRLGLQFQPEMTPLIFSGYIRDGIEFTTKQASVDNARTL
                     ARQLDALVGADTAARAVYFGRLGYGPVPVSRSTRLPLARLMTGGGSPDASPQKS"
BASE COUNT          760 a         1606 c         1672 g          711 t
ORIGIN      
        1 ggcgcaatga gcccggtgcg gccgcagggt ccggacccgc gggcttcagt cgatgccctg
       61 cgtgacgcgc ttcatgtagt ccttgttgaa cagcttgttc ggcagctcgc gcggcttcat
      121 cgcggagtaa tcgagcacgg agcggtcacc gcgcttgacc gcatcctcca tgaccagctg
      181 ggtcggacgc acggtgttgt cgagctttgc gtagttcagg tactgggcgg tcttcagcag
      241 ccggccggac agcgcataga actcggcctt cagcggctgg ttgccgctgc gctcgaccca
      301 cagcagcacg cggctgtagg tgacgctgcc gtcgatggcg gtcagctcca gcaccgcgta
      361 cttgcgcttc tggatggtct cgtcgcgcag cacgcgggcg ttgtagtcgc ccgagaagtt
      421 cgcgcgtgcc agatcgccgt tggcgacctg gccggtcagg cgctgcgaca gcggcaggcg
      481 caccggctgc gacaggcgcg gcgagtacac ccacaggtcg cgcccgcgca tcagcagcac
      541 ctggccgcgc tccgaggccg gggcggtagt gatgacgagg gtgttttcgt tacccttcga
      601 caggatctgg taatcgcgga cctcggtttc gcctccgggc gtggtcgagc tgatccgcac
      661 gttcacctcg aagtcgctgc gcggaaaccg gacctgatcg gcggcctcca gcatcgcgag
      721 ggcttcgagg tccacttcct gtgcccgcgc cgccggcatc gccagggtgg cggcgcacag
      781 cagcagcagg gaaggcagac gcagtacggt cgcacgcatc ggcatactct ccaggcatcg
      841 ggtacacaac gggtacgccc ggtcgcaggg ccgggcaggg acacggcgcg agccgcggga
      901 tgaagaacgg gtcatgagca tagtacgcat cgaaaacgtc tcgaaacgct accgcctcgg
      961 cgagcaggac gtgacggcgc tgcacggcgt cagcctgcgc gtcgagccgg gcgagttcgt
     1021 ggcgctggcc ggaccctccg gcagcggcaa gacgacgctg ctgaacatca tcggctgcat
     1081 cgacacgccg agttccgggc gcgtcatcgt cgatggccat gacgtgaccg gccgcgatcc
     1141 ggaccagctc gccgaactgc gcgcccgcac gctcggcttc atcttccaga ccttcaacct
     1201 gctgccggtg ctgtcggccg aggaaaacgt cgagtacccg ctgatcaaca tccagcacat
     1261 cccccgggcc gagcgccgcc agcgcgtcgc gtacttcctc gacatggtcg gactcgcgcg
     1321 gttcgcacgc catcgcccga accagctttc gggcggccag cgccagcgcg tcgcgatcgc
     1381 ccgggcgctg gcgaccaagc cgaagatcgt gcttgccgac gagccgaccg cgaacctcga
     1441 tcaccagagc ggcgacggta tcctgcagct gatgaagcac atcaacgagc agtccggcac
     1501 caccttcatc ttttcgacgc acgacgcccg cgtgatggcg atggccagcc ggctcgtgcg
     1561 catcagtgac ggcgccatcg tcggcgagga gacccgatga cgaaccatcc gaaccggtcc
     1621 ggccgcatcg ccctgggcat cgcactgctg gcggtcacgc cggcggctgc cgcgcagcag
     1681 gacgtgaagc tcagcggcta cctgtcgaac gagaccgcgt acaccttcgg cggcgagaat
     1741 cactggtcga agtcccgcaa cacgctgaac ctggccgcca ccggcagctt cagcgagtcg
     1801 atctcgtgga aggcgagcgg ctggctgcgc tgggacccgg ttgtccagca ctcggatttc
     1861 tatccggaca aggtgcagga cgaccgcggt ttcgaggggc tggtgcgcga gaccttcctc
     1921 gatgtgagcc tcggggactg ggacctgcgc ctcgggcgcc agaacatcgt ctggggcgag
     1981 atggtcggcc tgttcttcgc cgacgtggtc tcggcgcgcg acatgcgcga gttcgtgctc
     2041 gccgacttcg agcagctgcg catcccgcag tgggcggcgc gcgccgagta cttcaaggac
     2101 gacttccacg ccgagctcgt gtggatcccg tacctgacct acgacaacat cggcgagccc
     2161 ggcgacgact tctacccgta tccgccggcc ttcccggggc tgggctaccg catcctcgat
     2221 gaaaagaaac cgcgcggcgg tgacagcgac gatgccgccg gcgtgcgcct cggctggatc
     2281 gtcgatggct gggacatcaa cggttactac taccgcagca ccgacgtgca gccggccttc
     2341 cagcgcagct tcggcctggt cggcggcgtg ccgacgctga cctacacgcc gacgcacaac
     2401 cgcatccagc agtggggcct gaccgtcggc aaggacatcg gcgacgggct ggtgttcaag
     2461 gccgaggcga tctacacgat gggccggcgc tacgacgtgc tgcgcctgag cgatgccgac
     2521 ggtctggtgc gcaaggacac gctcgattac gtgctcggtc tcgactggac gccggacgcc
     2581 agcaaccgcc tgaacctgca gttcttccag cgcttctatc aggaccacga cccggacatg
     2641 gtgccggaga gcgtcgagag cggtgtcagc ctgttctatt cgaccaaggt ctggggcgac
     2701 cggctggagc cggaaatcac cttgatcgag agcctcaacc gcggcgactg gctgctgcgg
     2761 ccgcggctga cctggaacgt cagcgccgac ctgaaggcca gcttcggcgc cgacatcctg
     2821 aacggcccgc cggatgcgct gttcggccgc ttcaccaacg gcgaccgcgt ctacgccgag
     2881 gtccgctacg acttctgagg gctggcgtcc gggctgccgc cgccggtcat cagccgcgcg
     2941 agcggcaggc gcgtcgagcg cgataccggc accgggccgt agccgaggcg gccgaagtac
     3001 acggcgcgcg ccgcggtgtc ggccccgacc agtgcatcga gctggcgggc gagcgtgcgg
     3061 gcgttgtcga ccgaagcctg ctttgtcgtg aattcgatgc cgtcgcggat gtagccggag
     3121 aagatcagcg gcgtcatctc cggctggaac tgcaggccga ggcgggtcgc ggtcagccag
     3181 aagcgctgca ttgcggcgcc ggcggcgaga tagtcggcga gcgtctgcgg tgcctgttcg
     3241 gcaatgatca ggaaatgcgc ggcgcagccg agcgccggtg cgaagtcgag ctgcagccgc
     3301 ggcagccagg tgccggcgaa ccagcggttc aggaagtcga cgcgctccca gctcttcatc
     3361 gcccagcgca tcagcgcgag cccgaccgga tcgagcccga ccgcggcccc gggcagccgg
     3421 tcctcgctgt actgcacgcc gaactcgatg atgtcgcggt gcgtcgggta ggcctcgggc
     3481 gtcgtcagcc ggatgtgggc gttgcgaaac agcagcttcg cgacatcgag gcgctgatcc
     3541 ccttccagcc agcgcagcga gtagccggga ccgagcgcct gcaggaaggc cgccttcacg
     3601 gcgtccgtga gcggccggcg cgacagcggc cggcgctgcg tgcagcgtgc gcggatcgcg
     3661 gtttccagcg gatggcgcac ggccgccgct gccggcgtga actgcaggcg gaacatcggc
     3721 gcaaacgccg gcgtcgtgtc cccgacgccg ggcagcgttt cgatgtcgag cgcaagcccc
     3781 gcgcccgggg cggcgatgcg cgcggtttcc agcagcgcgc caagggcgag ctggctcggc
     3841 cggccgcgca ggtcgtagat cacgtgatcg cgcgtgtcgc agccgtgcac gatcacggtc
     3901 agcgggtcga cggcctcgaa gcgccacggc tgggtgttgt cgccgctcgg ggcccagctc
     3961 gccagttcga gcacgcgtgc gacgcgcggg tccagcccgg ccggtgccgc aggcgcggca
     4021 cgcgccgggg ccggcaggct gcgggcgcgg cgcagcagcg tgcgcatgat cgccagcgtc
     4081 aggcggttca gcgggtggcg gttgccgccc gggcgccagg tgcgcgacag ccggttgcgg
     4141 taggcatcga agtgcaggcc ccacggcgcg gcgcgcaccg ggccgcggcc gagcagcagc
     4201 ttcagcgatt cggtcacgag cacgccggcg gcgagttcgc aggccatcgg cgtcgacggt
     4261 ccgcgcttct cgacgaagtt gatcgtcgag cgatcgacca gatagccctg ctgcagcatc
     4321 gtcggcgaca gcccgaccag gaagcgcgcg tactgttcct cctcgggttg gccctcgacc
     4381 tggaagtagt cctcgaagct catgcgcccg ggcaggaagg tcagcaccgc cgtgcccatg
     4441 ccgagcggcg cggccgtgat cgccgggatg ccgcgggcgt gggcggccgc gaacaccttg
     4501 cgccgcaccg gcagcacgaa gaaatccagc gcatcgacgt acaggtcgac gccgtcgaag
     4561 aaggcatcga ggttgtcgtc ggtgacgccg tccgcaaagc gccggatatc gagttccgga
     4621 ttgatcgcga gggcggcctc ggccatcacg tcgagcttcg gccgatcgag cgtcgccatc
     4681 gtcgcgccgg cctgacggtt gaagttgtgc agctcgaagc ggtcgaagtc ggcgaggtgg
     4741 aaggcgccg
//
LOCUS       DAOQJH010000108         4726 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_10723_length_4726_cov_3.980090, whole genome shotgun sequence.
ACCESSION   DAOQJH010000108 DAOQJH010000000
VERSION     DAOQJH010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 4726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4726
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10723_length_4726_cov_3.980090"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..409)
                     /locus_tag="PLQ18_03695"
     CDS             complement(<1..409)
                     /locus_tag="PLQ18_03695"
                     /inference="COORDINATES: protein motif:HMM:NF019095.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HNE00787.1"
                     /translation="MPRLPRLLSALAFAAVAHGAQAIPINYVATLSGAAEAPPNASAG
                     TGSAHVVIDTDAHTYAIHFDFSGLTGTTTAAHIHGPTASPGTGTAGVMTTTPAFPGFV
                     TGVSSGSYDQLFDMTVASTFNPAFVTAQGGLANA"
     gene            complement(540..1805)
                     /locus_tag="PLQ18_03700"
     CDS             complement(540..1805)
                     /locus_tag="PLQ18_03700"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HNE00788.1"
                     /translation="MSVAAREACSVRRFRVRSCATLLLATLPPVAAADVVGIDASIFW
                     LLVSVMAITGLAALFVQQRMQRRLDRAERGLQRAVSEQREAERRYRLLAAHGSELIWT
                     MSLGTPQFTYLSPALERLCQFAPSSMQNRPPEELCTLESAALLSAEHARLSRQAGDAS
                     IPEVRFELELRCADGTAIWTEARLGVLRDESGTATAIYGVVSDMTEQRKLRARLDFLA
                     HHDALTGLPNRMLFFDRLERAVAAARRDGTRLALLYLDLDGFKEINDSLGHHAGDTLL
                     MQVALRLSASVRDSDTVARMGGDEFTVILRTVRDAPSVARITEKILTALSEPIAIDAQ
                     RVRIGVSIGASQFPEHGDQVGTLVALADAAMARSKRGGRNRWSMAIPDTPVLKSVPVE
                     APPAETPPPEGAGSVRPVLAAVRNPLSES"
     gene            complement(1970..2392)
                     /locus_tag="PLQ18_03705"
     CDS             complement(1970..2392)
                     /locus_tag="PLQ18_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNE00789.1"
                     /translation="MHQAIRIRELDHVVLRVRDPARMQHFYETVLGCPVERRLDIGLI
                     QLRAGASIIDLVPFDSELGRQGGHAPDHDGPNLDHFCVRVEPWDEAAIRAHLAAHGLA
                     PGPTERRYGAEGMGPSIYLHDPEGNVVELKGPPDGQSA"
     gene            complement(2410..3804)
                     /gene="fumC"
                     /locus_tag="PLQ18_03710"
     CDS             complement(2410..3804)
                     /gene="fumC"
                     /locus_tag="PLQ18_03710"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005346492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HNE00790.1"
                     /translation="MNMRIEHDSLGDVEVPDERLWGAQTQRSLEHFRISTEKMPPELI
                     RALARLKRAAALANAGLGVLPEPVAQAIVQAAGEIIDGGHADEFPLAVWQTGSGTQTN
                     MNLNEVIANRASELLGGPRGQGRKVHPNDAVNAGQSSNDAFPSAMHLAAVEAVHRQVL
                     PALATLRETLADKAVACAGVIKIGRTHLQDATPLTLGQEMSGWVAQLAHAERHLTDAL
                     PHLCELALGGTAVGTGLNAHPQFAERAIAELARSTGLPFVPAPNRFEALAAHDALVHA
                     HGVLKSLAAALVKIANDVRWLASGPRSGLGELTIPENEPGSSIMPGKVNPTQCEALTM
                     ACAQVFGNDVAIGFAGSQGNFELNVYKPLIIHAFLQSTRLLADGMDSFRRHCALGIEP
                     NLPRIRELLDRSLMLVTALNRHIGYDRAAAIARKAHTGGLTLKEAALALGFVSAEQFD
                     EWVRPEQMLGPGTD"
     gene            complement(3854..>4726)
                     /gene="acnA"
                     /locus_tag="PLQ18_03715"
     CDS             complement(3854..>4726)
                     /gene="acnA"
                     /locus_tag="PLQ18_03715"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011134676.1"
                     /note="catalyzes the conversion of citrate to isocitrate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HNE00791.1"
                     /translation="SYRRRYGAIDGGPLWQDIPVPNGARYHWPDSAYIARPPFFDGFT
                     RTPSPPADIRDARALLILGDSVTTDHISPAGSFRADSPAGRYLQSLGVPPAEFNSYGS
                     RRGNHEVMIRGTFANVRIRNRMLAPGADGKPVEGGWTLLDGERVTVYDAAMEYGRRGV
                     PTLVFAGEEYGTGSSRDWAAKGTLLLGVRAVVARSYERIHRSNLVGMGVLPLQFIGED
                     SAHTLNLGPDDRFDLPPLASLKPQQTLELTIRSPDGSVRQVPVLCRIDTPIELTCFRH
                     GGILPWVLRQLLDD"
BASE COUNT          660 a         1585 c         1767 g          714 t
ORIGIN      
        1 cggcattggc gaggccgccc tgcgcggtca cgaaggcagg gttgaaggtc gaggccaccg
       61 tcatgtcgaa gagctggtcg tagctgcccg agctgacgcc ggtcacgaag cccggaaacg
      121 ccggggtcgt cgtcatcacg ccggccgtgc cggtaccggg cgaggcggtc gggccgtgga
      181 tgtgggcggc ggtggtggtg ccggtcaggc cgctgaagtc gaagtggatc gcgtacgtat
      241 gcgcatcggt atcgatgacc acgtgcgcgc tgccggtgcc ggccgaggca ttcggcggtg
      301 cttcggcggc accgctcagc gtcgcgacgt agttgatcgg gatggcctgc gcaccatggg
      361 cgacggcggc gaaggcgagg gcggataaca ggcggggaag gcggggcatg ggcaaggcac
      421 tccctgatgg cttttgttga ctgggttcca cgggcatgtg ccggcagggt gtgatccggc
      481 acagcgccag cgtagtcgcc ggcagtcgca gcggaagcag ggcagccggg gcggccggct
      541 tacgattcgg acaggggatt gcgtaccgcg gccagcaccg gacgcacgct gccggcgccc
      601 tccggtggcg gtgtttcggc aggcggggct tccaccggca ccgacttcag caccggcgta
      661 tccgggatcg ccatgctcca gcggttgcga ccgccgcgct tgctgcgcgc catcgctgca
      721 tcggccagtg ccaccagcgt gccgacctga tcgccgtgct ccggaaactg gctggcgccg
      781 atgctgacgc cgatgcgcac gcgctgggcg tcgatcgcga tcggttcgga cagcgcggtg
      841 aggatcttct cggtgatgcg cgcgaccgag ggggcgtcgc gcaccgtgcg caggatcacc
      901 gtgaactcgt ccccgcccat gcgcgcgacc gtgtcggaat cgcggacgct ggccgagagc
      961 ctgagcgcca cctgcatcag cagcgtgtcg ccggcatggt ggccgaggct gtcgttgatc
     1021 tccttgaaac cgtcgaggtc gaggtacagc agcgccagcc gggtgccgtc gcggcgggcg
     1081 gcggcgaccg cgcgctccag ccggtcgaag aacagcatgc ggttcggcag cccggtcagc
     1141 gcgtcgtggt gggcgaggaa atcgagccgg gcacgcagct tgcgctgctc ggtcatgtcg
     1201 ctgacgacgc cgtagatcgc agtcgcggtc ccgctctcgt cgcgcagcac gccgaggcgc
     1261 gcctcggtcc agatcgccgt gccgtcggcg cagcgcagtt cgagttcgaa gcggacttcc
     1321 ggaatcgacg catcgccggc ctgacggctc agccgggcgt gttcggcgct cagcagcgca
     1381 gccgattcga gcgtgcagag ttcctccggc ggccggttct gcatgctcga tggcgcgaac
     1441 tggcacagcc gttcgagggc cgggctcagg tacgtgaact gcggcgtccc gagcgacatc
     1501 gtccagatca gctcgctgcc gtgcgcggcc agcagccggt aacggcgttc ggcctcgcgc
     1561 tgctcgctga cggcgcgctg cagcccgcgt tcggcacggt cgagccggcg ctgcatgcgc
     1621 tgctgcacga acagcgccgc cagaccggtg atcgccatga ccgagaccag cagccagaag
     1681 atcgaggcat cgatgccgac cacatcggcc gccgccaccg gcggcagggt ggcgagcagc
     1741 agtgtcgcgc agctgcggac ccggaatcgg cggacggagc aggcttccct ggcggcaacg
     1801 gacatcgggc ggcggcctcg gcgaacacgg tggtctggac cccgtggcgg accggcacgg
     1861 gacgggtgtc gctaccatgc cagaagcgta cgaacatccg cagccggtaa cgggaaattt
     1921 cgcatggccg gccggccggt acgccgggtg gcaccggcct gcgcaggact caggcgctct
     1981 gcccgtccgg cggacccttc agctcgacga cgttgccttc cgggtcgtgc aggtagatcg
     2041 acggccccat gccttcggcg ccgtagcggc gttcggtcgg gccgggcgcg aggccgtggg
     2101 cggccagatg cgcgcggatc gcggcctcgt cccagggctc gacgcgcacg cagaagtgat
     2161 cgaggttcgg gccgtcgtga tccggcgcgt gcccgccctg acggccgagt tcgctgtcga
     2221 atggcaccag atcgatgatg gaggcaccgg cgcgcagctg gatcaggccg atgtcgagcc
     2281 gccgctcgac cgggcagccg agcacggttt cgtagaagtg ctgcatgcgt gccggatcgc
     2341 gcacgcgcag cacgacgtga tcgagttcac ggatgcggat ggcctgatgc atcgtgcggc
     2401 tcccgttcct cagtcagtac cggggccgag catctgctcc gggcgcaccc attcgtcgaa
     2461 ctgttcggcg ctgacgaagc cgagtgccag cgcggcctcc ttcagcgtca ggcccccggt
     2521 gtgcgccttg cgcgcgatcg cagcggcgcg gtcgtagccg atgtggcggt tcagcgccgt
     2581 caccagcatc agcgaacggt cgagcagctc gcggatgcgc ggcaggttcg gttcgatgcc
     2641 gagcgcgcag tggcggcgga agctgtccat gccgtcggcg agcagccgcg tgctctgcag
     2701 gaaggcatgg atgatcagcg gcttgtagac gttcagctcg aagttgccct gcgagccggc
     2761 gaagccgatc gccacgtcgt tgccgaacac ctgcgcgcag gccatcgtca gcgcctcgca
     2821 ctgcgtcggg ttgaccttgc cgggcatgat cgagctgccc ggctcgttct ccgggatcgt
     2881 gagctcgccg aggcccgagc gcggccccga ggcgagccag cgcacgtcgt tggcgatctt
     2941 gaccagcgcc gcggccagcg acttcagcac gccgtgcgcg tgcaccagcg catcgtgcgc
     3001 ggccagcgcc tcgaagcggt tcggtgccgg cacgaagggc aggccggtgc tgcgcgccag
     3061 ttccgcgatc gcgcgttcgg cgaactgcgg atgggcgttg aggccggtgc cgaccgcggt
     3121 gccgccgagc gcgagttcgc agaggtgcgg cagggcatcg gtcagatggc gttcggcgtg
     3181 cgcaagctgc gcgacccagc ccgacatctc ctgcccgagc gtcagcggcg tggcgtcctg
     3241 caggtgcgtg cggccgatct tgatgacgcc ggcgcaggcg acggccttgt cggcgagcgt
     3301 ctcgcgcagc gtcgccagcg ccggcagcac ctgccggtgc acggcctcga ccgcagcgag
     3361 gtgcatcgcg ctcgggaagg cgtcgttcga ggactggccg gcgttgaccg catcgttcgg
     3421 gtgcaccttg cggccctggc cgcgcgggcc gccgagcagc tcgctggcgc ggttggcgat
     3481 cacctcgttg aggttcatgt tggtctgcgt gccggagccg gtctgccaga ccgcgagcgg
     3541 aaattcatcg gcatggccgc cgtcgatgat ctcgccggcc gcctgcacga tcgcctgcgc
     3601 gacgggctcc gggagcacgc cgagcccggc gttggcgagc gcggcggcgc gcttgaggcg
     3661 cgccagcgcc cggatcagct ccggcggcat cttctcggtc gagatgcgga agtgctccag
     3721 cgagcgctgc gtctgtgcgc cccagagccg ttcgtcgggg acttccacgt cgccgaggct
     3781 gtcgtgttcg atgcgcatgt tcatcgggat cactccgggg aagcagccgt ccggcctctg
     3841 ctcgtgactg ccctcagtcg tcgagcagct ggcgcagcac ccagggcagg atgccgccgt
     3901 ggcggaagca ggtgagttcg atcggcgtgt cgatgcgaca gagcaccggc acctggcgca
     3961 cgctgccatc ggggctgcgg atcgtcagtt ccagcgtctg ctgcggcttg aggctcgcga
     4021 gcggcggcag gtcgaagcgg tcgtccgggc cgaggttcag cgtgtgggcg ctgtcctcgc
     4081 cgatgaactg cagcggcagc acgcccatgc cgaccaggtt cgagcggtgg atgcgctcgt
     4141 agctcctcgc gaccaccgca cgcacgccga gcagcagcgt gcccttggcc gcccagtcgc
     4201 ggctcgaacc ggtgccgtat tcctcgccgg cgaacaccag cgtcggtacg ccgcgccggc
     4261 cgtattccat cgccgcgtcg tagaccgtca cccgctcgcc gtcgagcagc gtccagccgc
     4321 cctcgaccgg cttgccgtcc gcaccggggg ccagcatccg gttgcggatg cgcacgttcg
     4381 cgaaggtgcc gcggatcatg acctcgtggt tgccgcgccg ggagccgtag ctgttgaact
     4441 cggccggcgg cacgccgagc gactgcaggt agcgcccggc cggcgagtcg gcgcggaagc
     4501 tcccggccgg cgagatgtgg tcggtggtca ccgagtcgcc gaggatcagc agcgcgcgcg
     4561 catcgcggat gtcggccggc ggcgagggcg tgcgcgtgaa gccgtcgaag aacggcggcc
     4621 gggcgatgta ggccgagtcc ggccagtggt agcgcgcgcc gttcggcacc gggatgtcct
     4681 gccacagcgg cccgccgtcg atcgcgccgt agcggcggcg gtagct
//
LOCUS       DAOQJH010000109         4708 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_10775_length_4708_cov_3.974640, whole genome shotgun sequence.
ACCESSION   DAOQJH010000109 DAOQJH010000000
VERSION     DAOQJH010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 4708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4708
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10775_length_4708_cov_3.974640"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..375)
                     /locus_tag="PLQ18_03720"
     CDS             complement(<1..375)
                     /locus_tag="PLQ18_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNE00792.1"
                     /translation="MLRMFQPLEVFIGLRYVRAKRSNHFISFISASSMLGIALGITAL
                     ITVLSVMNGFQQEVRERILAMTAHATISGVAGRLDDWREVRKAANANAEVVASAPYIR
                     AESMLSAGSATAGTIVQGVLPDE"
     gene            complement(469..834)
                     /locus_tag="PLQ18_03725"
     CDS             complement(469..834)
                     /locus_tag="PLQ18_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019398137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HNE00793.1"
                     /translation="MKLFHHLLKPLLGCLLGLWFTISVAAPLDINTATAAELATVMQN
                     VGPKKAEAIVAWREQNGPFKSVDELEKVPGIGPKIVEANRDRLTVDTPAAAATPAPAP
                     AMPAPAAAPAAPAAPVKKP"
     gene            complement(983..1711)
                     /gene="pyrF"
                     /locus_tag="PLQ18_03730"
     CDS             complement(983..1711)
                     /gene="pyrF"
                     /locus_tag="PLQ18_03730"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HNE00794.1"
                     /translation="MTTPSPQARDPRLIVALDYPQAAPALALAARLDPALCRLKVGKE
                     LFTAAGPALVEALQQRGHEVFLDLKFHDIPNTVAGAVRAAAGLGVWMVNVHASGGRRM
                     LEAARAAVAAPVRPPLLIAVTVLTSLERADLAETGLDIEPAAQVDRLAALSRDCGLDG
                     VVCSAQEAARLRALCGPAFRLVTPGIRPASSEAGDQRRTMTPADALAAGADYLVIGRP
                     VTGAPDPAAALADIAAGLANIGRT"
     gene            complement(1752..2909)
                     /gene="lapB"
                     /locus_tag="PLQ18_03735"
     CDS             complement(1752..2909)
                     /gene="lapB"
                     /locus_tag="PLQ18_03735"
                     /inference="COORDINATES: protein motif:HMM:NF008757.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HNE00795.1"
                     /translation="MLELLWLLLPVAAASGWWAAARSLERKETPPNDVFRGLNYLLDE
                     KPDRAIEVFGRMVEADPDAVDIQLTLGSLFRRRGEVDRALHIHSELLRRAQLSAGQRG
                     RARLELGEDYLRAGLFDRAEQLFSELLELPDYRSLALVRLLSIYEQEREWQRALDACD
                     EVERATGTPMRIVAAHYCCELAEEAVRASDRATARSWLDRALVHDERCARAAILRGRL
                     ELSDGNPAGALDAYTRVVTRAPEYLPEVLGPLQRCYAQLHRQADWVTLLRGLVNQNHV
                     GSVTGALAQALREREGPEAALVFLEAEVAQHPSTEGLRTLIDLRLAAEQPARAGDLEL
                     LLRIGRQVTDLGPRYRCDNCGFVGRTLHWSCPGCKRWNTVKPIPDRLRRNP"
     gene            complement(2920..3207)
                     /locus_tag="PLQ18_03740"
     CDS             complement(2920..3207)
                     /locus_tag="PLQ18_03740"
                     /inference="COORDINATES: protein motif:HMM:NF018058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HNE00796.1"
                     /translation="MFRIVGILLFLLVLVVGIEFSAVNSAPVTVNYLLGTVSLPLALV
                     VVAAFTVGILVAVAVGTLAMVPLRLRAASLRREVEARQFEIERLRARQPGT"
     gene            complement(3331..3660)
                     /locus_tag="PLQ18_03745"
     CDS             complement(3331..3660)
                     /locus_tag="PLQ18_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008247153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HNE00797.1"
                     /translation="MTKSELIELLARKQSHLSYKDVENAAKVMIEQMSQSLSGGDRIE
                     IRGFGSFSLHFRPPRVGRNPKTGDAVTLPGKFVPHFKPGKELRERVNASIENDDPRPT
                     ARIAEPA"
     gene            complement(3787..>4708)
                     /locus_tag="PLQ18_03750"
     CDS             complement(3787..>4708)
                     /locus_tag="PLQ18_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018174337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HNE00798.1"
                     /translation="ERNRVSLGLKQLGDDPWVALARRYPVGTRLFGKVTNIADYGCFV
                     EIEEGVEGLVHVSEMDWTNKNVNPGKVVSIGDEVEIMVLDIDEDRRRISLGMKQCQPN
                     PWDEFCATHNKGDRVSGKIKSITDFGIFIGLDGGIDGLVHLSDISWNVPGEEAVRDYK
                     KGQEVEAVVLAVDPERERISLGIKQLEKDPFSNFVADNPKGSIVKGRVASVDARGAVI
                     DLGNGVEGTLRVSELARERIEDARTVLKDGEEIEAKFIGVDRKNRTITLSIKAKEYAE
                     EAEVLQDYSRKSATGATLGDLLKEQMGRED"
BASE COUNT          714 a         1551 c         1701 g          742 t
ORIGIN      
        1 ctcgtcgggc agcacgccct ggacgatggt cccggccgtg gcgctgccgg cgctcagcat
       61 cgattcggcg cggatgtacg gggcgctggc gacgacctcg gcgttggcgt tggcggcctt
      121 gcgcacctcg cgccagtcat cgagccggcc ggcgacaccg gagatcgtcg cgtgcgcggt
      181 catcgccagg atgcgttcgc gcacctcctg ctgaaagccg ttcatgaccg acagcaccgt
      241 gatcagcgcc gtgatgccga gcgcgatgcc gagcatcgac gacgcggaga tgaacgagat
      301 gaagtgattg gagcgtttgg cccgcacgta acgcaggccg atgaacactt ccagcggctg
      361 gaacatcctg agcatgcgag gggataccgg gtgacgggga cggcccgccg cccggcgaga
      421 gggcggcggg gccggcggag tataccggcc ggccccgcgg ccgatggctc aaggcttctt
      481 gactggagct gccggtgcag ccggagcggc cgcaggcgca ggcatcgccg gggccggggc
      541 cggggtggca gcggcggccg gcgtgtcgac ggtcaggcgg tcgcggttgg cctcgacgat
      601 cttcggcccg atgcccggga ccttctccag ctcatcgacc gacttgaacg ggccgttctg
      661 ttcgcgccag gcgacgatgg cctcggcctt cttcggaccg acgttctgca tgacggtcgc
      721 gagttccgcg gcggtggcgg tgttgatgtc gagcggggct gcaacggaaa tggtgaacca
      781 caggccgagc aggcagccca gcagcggctt gagcaggtga tggaacagct tcatgacgga
      841 ttcctcccga tggatgtccg gatggccggg aggcggggcc ggtgcgatcc ggaccctgag
      901 gcttccctgc catgtcagcc ggagggctga cggggttcag gctagatcgc cgtcctgaaa
      961 cttcggttgc atggatgaaa cttcatgtcc tgccgatatt tgcgagcccg gcagcgatgt
     1021 cggcgagcgc agcggccggg tcgggcgcgc cggtgaccgg ccggccgatg accagataat
     1081 cggcaccggc cgcgagcgcg tcggccgggg tcatcgtgcg ccgctggtcg ccggcctcgc
     1141 tgctcgccgg ccggatgccg ggcgtcacca gccggaaggc cgggccgcac agcgcccgca
     1201 gccgggcggc ttcctgcgcc gagcagacca cgccatcgag gccgcagtcg cgcgacaacg
     1261 cagccagccg gtcgacctgc gcggccggct cgatgtcgag tccggtttcg gcgagatcgg
     1321 cacgctccag actggtcagc accgtcaccg cgatcagcag cggcggacgc accggggcag
     1381 cgacggccgc gcgcgcggct tcgagcatgc gccgtccgcc cgaggcatgg acgttgacca
     1441 tccagacccc gagcccggcg gccgcgcgca cggctccggc gacggtgttc gggatgtcgt
     1501 ggaacttgag gtcgaggaac acctcatggc cgcgctgctg cagggcctcg accagcgccg
     1561 gtccggcggc cgtgaacagt tccttgccga ccttgagccg gcacagcgcc gggtccagcc
     1621 gtgccgccag cgccagcgcc ggcgcggcct gcgggtaatc gagcgcgacg atcaggcgcg
     1681 ggtcgcgcgc ctgcggggac ggggtggtca tcggggcaga atctccggcg ggggacgggc
     1741 aggcgggggc ttcagggatt gcggcgcagg cgatcgggga tcggcttgac ggtgttccag
     1801 cgcttgcagc ccggacagga ccagtgcagc gtgcgtccga cgaagccgca gttgtcgcag
     1861 cggtaacgcg ggccgagatc ggtcacctgc cggccgatgc gcagcagcag ttcgaggtcc
     1921 ccggcgcgcg ccggctgctc ggcggcgagt cgcagatcga tcagcgtgcg cagcccctcg
     1981 gtcgaggggt gctgcgcgac ctcggcttcg aggaagacaa gcgcagcctc cggaccttcc
     2041 cgttcgcgca gcgcctgcgc gagggctccg gtgacactgc cgacgtgatt ctggttgacc
     2101 aggccgcgca gcagtgtcac ccagtcggcc tgccggtgga gctgcgcgta gcagcgctgc
     2161 agggggccga gcacttcggg caggtattcc ggggctcgcg tgaccacgcg cgtataggcg
     2221 tcgagcgcac ccgccggatt gccgtccgaa agctccagcc ggccgcgcag gatcgcggcc
     2281 cgcgcacagc gctcgtcatg gaccagggcg cggtcgagcc agctgcgggc cgtggcgcga
     2341 tcactggcac gcacggcctc ttcggccagt tcgcagcagt aatgtgcggc cacgatgcgc
     2401 atcggcgtgc cggtggcccg ctcgacctcg tcgcaggcgt cgagtgcccg ctgccactcg
     2461 cgctcctgtt cgtagatcga cagcagtcgc accagtgcca gcgagcggta atcgggaagt
     2521 tcgagcagtt cgctgaacag ttgctcggcg cggtcgaaca ggcctgcgcg cagatagtcc
     2581 tcaccgagtt cgaggcgcgc acggccgcgc tggccggcac tcagctgcgc acggcgcagc
     2641 agctcgctgt ggatgtgcag cgcgcggtcg acctcgccgc gccggcggaa caggctgccg
     2701 agcgtcagct ggatatcgac ggcatcgggg tcggcctcga ccatgcggcc gaagacctcg
     2761 attgcgcgat cgggcttctc atcgagcaga tagttgaggc cgcggaatac gtcgttcggc
     2821 ggcgtctcct tgcgctcgag actgcgtgcc gcggcccacc agcccgaggc tgccgccacc
     2881 ggcagcagca gccagagcag ttcgagcatg cggtccggct caggtgcccg gctgcctggc
     2941 gcgcaggcgc tcgatctcga actgccgggc ctcgacctca cggcgcagcg acgcggcgcg
     3001 caggcgcagc ggcaccatcg ccagcgtgcc gaccgcgacc gcgaccagga tgccgaccgt
     3061 gaaggcagcg accacgacca gcgccagcgg cagtgacacc gtgccgagca ggtaattgac
     3121 ggtcaccggc gcggagttga ccgccgagaa ttcgatgccg acgacgagca cgagcaggaa
     3181 cagcaggatg ccgacgatgc gaaacatgga tcagggtgct ccacgggcag caggacggac
     3241 gccatcccgg cgtcggggac agacggaaat tggctcagga cagcacggac ggcaccgcta
     3301 ccggcatgcc gtccgcacga ggacgggtgt tcaggccggt tcggcgatgc gggcggtcgg
     3361 gcgcggatcg tcgttttcga tcgaggcgtt gacgcgctcg cgcagttcct tgccgggctt
     3421 gaagtgcggc acgaacttgc cgggcagcgt cacggcgtcg ccggtcttgg gattgcgacc
     3481 gacgcgcggc ggccggaaat gcagcgagaa actgccgaag ccgcggattt cgatgcggtc
     3541 accgcccgac agcgactggc tcatctgttc gatcatcacc ttggcggcgt tttcgacgtc
     3601 cttgtaactg aggtggctct gcttgcgggc cagcagttcg atcagttccg atttggtcat
     3661 ggatgatgtt ccggcagtac tcgaaaaatg gggttccggc ggttctgcgg ggcagcggcg
     3721 gatcgccgcc atggagtggt ccgggacggt gccgatgccg tcccggacca cgggctcgcg
     3781 cggggatcag tcctcgcgac ccatctgctc cttcagcagg tcgccgagcg tcgcgccggt
     3841 ggcggacttg cggctgtagt cctgcagcac ctcggcttcc tcggcgtatt ccttggcctt
     3901 gatcgacagc gtgatcgtgc ggttcttgcg atcgacgccg atgaacttgg cctcgatctc
     3961 ctcgccgtcc ttcagcaccg tgcgggcatc ctcgatgcgc tcgcgcgcca gttcggagac
     4021 acgcagcgtg ccttcgacgc cgttgccgag atcgatcacc gcgccgcggg catcgaccga
     4081 ggcgacacgg cccttgacga tgctgccctt cgggttgtcg gcgacgaagt tcgagaacgg
     4141 atccttctcg agctgcttga tgccgagcga gatgcgctcg cgctccggat ccacggcgag
     4201 cacgaccgcc tcgacttcct ggcccttctt gtagtcacgc acggcctctt cgccggggac
     4261 gttccaggag atgtcggaca ggtgcacgag gccgtcgatg ccgccgtcga gaccgatgaa
     4321 gatgccgaag tcggtgatcg acttgatctt gccgctgacg cgatcgccct tgttgtgggt
     4381 cgcgcagaac tcgtcccacg ggttcggctg gcactgcttc atgccgagcg agatgcggcg
     4441 gcggtcctcg tcgatgtcga gcaccatgat ctcgacttcg tcgccgatgc tgacgacctt
     4501 gccggggttg acgttcttgt tggtccagtc catttcggag acgtggacca gaccctcgac
     4561 gccttcctcg atctcgacga agcagccgta gtcggcgatg ttcgtgacct tgccgaacag
     4621 gcgggtgccg accgggtagc ggcgggcgag cgcgacccac ggatcgtcgc cgagctgctt
     4681 caggccgagc gagacgcggt tgcgctcg
//
LOCUS       DAOQJH010000110         4693 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_10834_length_4693_cov_3.364812, whole genome shotgun sequence.
ACCESSION   DAOQJH010000110 DAOQJH010000000
VERSION     DAOQJH010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 4693)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4693)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4693
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10834_length_4693_cov_3.364812"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..859
                     /locus_tag="PLQ18_03755"
     CDS             <1..859
                     /locus_tag="PLQ18_03755"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HNE00799.1"
                     /translation="DLTGLHRDGSEFPVEVGLSPITLHGETCMLAAITDITERKKMEL
                     DLRKTNANLEEFTYVASHDLRSPLRGISDLLSWVREDLGEAPAPAVVKNLDRITLRVQ
                     RMEQLIDNLLSYARAGRAATDFRELELDPLLDRVCELVQLPPGFELRRELVEIRLFSP
                     PTPLETVLRNLIANAIKHHDREHGLVRIGCTAEGSYCHLTVTDDGPGIPKPAQERIFK
                     LFQTLTAAERGGSGIGLAVARRLAETHGGRLELTSDPELRQTTFHVWWPRFQRRDLHD
                     DKNESAARR"
     gene            828..1250
                     /locus_tag="PLQ18_03760"
     CDS             828..1250
                     /locus_tag="PLQ18_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007641595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE00800.1"
                     /translation="MTRMNLLLVDDDDVATEAVLRSLAKCDLKPVVVTAEDGLEALHV
                     LRGTHAGKRIDRPYLVLLDLNMPRMNGFEFLENLRADERLRDTVVFVLTTSDADTDRT
                     RAYHENIAGYMVKSCIGPQFRHLVSLLEVYQQTVRLPG"
     gene            1257..3020
                     /locus_tag="PLQ18_03765"
     CDS             1257..3020
                     /locus_tag="PLQ18_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012070477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing response regulator"
                     /protein_id="HNE00801.1"
                     /translation="MPDFRIPLPWPETTDKAQLRLLVVDDDDVDRERVRRMLLRTAPD
                     ATVVEACDPEETLALLQEQAFDCMIVDYRLGRLTGLDLMDLIREVVPQRCATIMVTGL
                     GDEEIAGAAVRSGINDYLTKNQLDPVGLMRSVTGAMRRTALERRLHELAYYDALTGLA
                     SRTLLTDRLQQLIARRAREPGMLAALAYIDLDRFKPINDRHGHHAGDQVLGVIAQRLK
                     GAIRASDTAARLGGDEFALLLTEVESAEDCEAVLERISRLLREPIRLDGRSDGQTVQV
                     TASIGVVLIQDDSLEADSVLRHADHMMYEVKNAGREGVRFFDPQVERRLKAAHDELEA
                     VAAGLRNGEFELFYQPQIDLATRAPTGLEALIRWRHPQRGLLAPDAFWNALQHKKLSA
                     TLGEWVIASACRQIDAWLAAGLRLKVSVNVSVHHLLHPSFAARLGAILAATPSVPPGY
                     LELEILETVAIDDFESTVAVIEACKALGVSVALDDFGTGYSSLTHLRSLPLDALKIDR
                     SFIASMLDSPSDQAIVLGILGLCRAFNRRAIAEGVESEAHIRRLVELGCHCGQGYGIA
                     RPMPAAEVAGWIERQRACVAA"
     gene            complement(3036..3707)
                     /locus_tag="PLQ18_03770"
     CDS             complement(3036..3707)
                     /locus_tag="PLQ18_03770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE00802.1"
                     /translation="MHRRPPVASLLAPLAATLLAALSAAPAHAVPVTDPAGDILSTYA
                     GVPAQGDVDVLFAEVIFNGSGFHLSATLADTLGQTPGALYVWGFDRGAGTERFATLPG
                     GVGVLFDSVVVIDALGTATVRTLAPGVAVTATLTASLVGDTLSVDVPLSALPSAGFAP
                     GAYTWNLWPRVGSGNNNQITDFAPDGNADPALANALVTVPEPASLALAAGGLGLIGRR
                     RLRHR"
     gene            3887..>4693
                     /locus_tag="PLQ18_03775"
     CDS             3887..>4693
                     /locus_tag="PLQ18_03775"
                     /inference="COORDINATES: protein motif:HMM:NF026220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HNE00803.1"
                     /translation="MAEAASAPSASAAGARRAGWLRQSRFWLLLAAALLLAGSTGLAL
                     HQPPHPDAWRPPPGWLDPAFWRWPFERNAFMRLPLLAAPINALHVADDGTTWLLTGAG
                     EILHMSGDGLRWQRQWPVATQAPAARAAGIAVPGIAVAHAQAMPPLDGAIGKGSEVPN
                     QAAPQSPAAPAYPKPGPTAPVQQTPMTGEAPQPRAPALNALHFLSDGRHGWAVGDAGT
                     LLVTDDGGEHWQRIELAEAGEKVLRSIWFVDAQYGWIVGDDGLRLRTIDGG"
BASE COUNT          706 a         1714 c         1621 g          652 t
ORIGIN      
        1 ggacctgacc ggcctgcacc gcgacggcag cgagttcccg gtcgaggtcg gcctgagccc
       61 gatcacgctg cacggcgaga cctgcatgct ggcggcgatc accgacatca ccgagcgcaa
      121 gaagatggaa ctcgacctgc gcaagaccaa cgccaatctg gaggaattca cctacgtcgc
      181 ctcgcacgac ctgcgctcgc cgctgcgcgg catcagcgac ctgctgtcct gggtgcgcga
      241 ggacctcggc gaggccccgg cgccggcggt cgtgaagaac ctcgatcgca tcacgctgcg
      301 cgtgcagcgc atggagcagc tgatcgacaa cctgctgagc tatgcccgcg ccggccgcgc
      361 cgccaccgat ttccgcgagc ttgaactcga cccgctgctc gatcgcgtct gcgagctcgt
      421 gcagctgccg ccggggttcg agctgcgccg cgagcttgtc gagatccggc tgttcagccc
      481 gccgacgccg ctggagacgg tgctgcgcaa cctcatcgcc aacgccatca agcaccacga
      541 ccgcgagcac ggcctggtcc gcatcggctg cacggccgag ggcagctact gccacctgac
      601 ggtcacggac gacgggccgg gcatcccgaa gccggcgcag gagcgcatct tcaagctgtt
      661 ccagacgctg accgccgccg agcgcggcgg cagcggcatc gggctggccg ttgcgcggcg
      721 actggccgag acccacgggg gccggctgga actgacttcc gaccccgaac tgcgccagac
      781 cacgttccac gtctggtggc cccgcttcca gcgccgagac ctgcacgatg acaagaatga
      841 atctgctgct cgtcgatgac gacgatgtcg ccaccgaggc cgtgctgcgc agcctcgcga
      901 agtgcgacct gaaaccggtc gtggtcaccg ccgaggatgg cctcgaagcc ctgcacgtgc
      961 tgcgcggcac gcacgcgggc aagcgcatcg accggccgta tctggtgctg ctcgacctga
     1021 acatgccgcg catgaacggt ttcgagttcc tcgaaaacct gcgcgccgac gagcggttgc
     1081 gcgacaccgt cgtgttcgtg ctgacgacct ccgatgccga caccgaccgc acgcgcgcct
     1141 accacgagaa catcgccggc tacatggtga agtcctgcat cggcccgcag ttccggcatc
     1201 tggtcagcct gctcgaggtc taccagcaga ccgtgcgcct gccgggataa gccgccatgc
     1261 ccgatttccg gattccgctc ccctggccgg agaccactga caaggcgcag ctgcggctgc
     1321 tggtggtcga tgacgacgat gtcgatcgcg agcgcgtgcg ccgcatgctg ctgcgcaccg
     1381 cacccgatgc caccgtcgtc gaggcctgcg atccggagga gacgctggcg ctgctgcagg
     1441 agcaggcgtt cgactgcatg atcgtcgact accggctcgg ccggctcacc gggctcgacc
     1501 tgatggacct gatccgcgag gtcgtgccgc agcgctgcgc gacgatcatg gtcaccggcc
     1561 tcggcgacga ggagatcgcc ggcgccgccg tgcgctcggg catcaacgat tacctgacca
     1621 agaaccagct cgatccggtc gggctgatgc gctcggtcac cggggcgatg cgccgcaccg
     1681 cgctcgaacg ccgcctgcac gagctcgcgt actacgacgc gctgaccggg cttgccagcc
     1741 gcacgctgct gaccgaccgc ctgcagcagc tgatcgcgcg gcgcgcgcgc gagccgggca
     1801 tgctggccgc gctcgcctac atcgatctcg atcgcttcaa gccgatcaac gaccgccacg
     1861 gccaccacgc cggtgatcag gtgctcggcg tcatcgccca gcgcctgaag ggcgcgatcc
     1921 gcgccagcga caccgctgcc cggctcggcg gcgacgagtt cgcgctgctg ctgaccgagg
     1981 tcgagtcggc cgaggactgc gaggccgtgc tcgagcgcat cagccggctg ctgcgcgagc
     2041 cgatccggct cgatggccgc agcgatggcc agaccgtgca ggtcaccgcc agcatcgggg
     2101 tcgtgctgat ccaggacgac agcctcgagg ccgacagcgt gctgcgccac gccgatcaca
     2161 tgatgtacga ggtcaagaac gccgggcgcg agggcgtgcg cttcttcgat ccgcaggtcg
     2221 aacggcgcct gaaggccgcg cacgacgagc tcgaagcggt cgccgccggc ctgcgcaacg
     2281 gcgagttcga gctgttctac cagccgcaga tcgacctcgc cacgcgcgca ccgaccgggc
     2341 tcgaagccct gatccgctgg cgccatccgc agcgcggcct gctcgcgccc gatgccttct
     2401 ggaacgcgct gcagcacaag aagctgtcgg cgacgctcgg cgaatgggtg atcgccagcg
     2461 cctgccggca gatcgacgcc tggctggccg ccggcctgcg gctgaaggtc agcgtcaacg
     2521 tctcggtgca tcacctgctg cacccgtcgt tcgcggcccg gctcggcgcg atcctcgcgg
     2581 cgacgccatc ggtgccgccc ggctatctgg agctcgaaat cctcgagacc gtcgcgatcg
     2641 atgacttcga atcgacggtc gccgtgatcg aggcctgcaa ggcgctcggc gtcagcgtcg
     2701 cgctcgatga cttcggcacc ggctactcgt cgctgacgca cctgcgcagc ctgccgctcg
     2761 acgcgctgaa gatcgaccgc agcttcatcg cctcgatgct cgacagtccg agcgatcagg
     2821 ccatcgtgct cggcatcctc ggtctctgcc gcgccttcaa ccggcgcgcg atcgccgagg
     2881 gcgtcgaatc cgaggcgcac atccgccgcc tcgtcgaact cggctgccac tgcggccagg
     2941 gctacggcat cgcccggccg atgccggcgg ccgaggtcgc cggctggatc gagcgccagc
     3001 gcgcctgcgt cgcggcctga gcgtacgcgg ccgcttcagc ggtgccggag gcgccggcgg
     3061 ccgatcaggc cgagcccgcc ggccgccagc gccagactgg ccggctccgg caccgtcacc
     3121 agcgcgttgg caagcgccgg atcggcgttg ccgtcgggcg cgaagtcggt gatctggttg
     3181 ttgttgccgc tgccgacgcg cggccacagg ttccacgtgt aggcgccggg cgcgaagccg
     3241 gccgagggca gcgccgacag cggcacgtcg acgctcagcg tgtcgccgac gaggctcgcc
     3301 gtcagcgttg cggtgacggc cacacccggc gccagcgtgc gcacggtggc ggtgccgagc
     3361 gcatcgatga ccacgaccga gtcgaacagc acgccgacac cgcccggcag cgtcgcgaag
     3421 cgctcggtgc cggcgccgcg atcgaagccc cagacgtaca gcgcgcccgg cgtctgcccg
     3481 agcgtgtcgg ccagcgtcgc gctcaggtga aaaccgctgc cgttgaagat gacttcggcg
     3541 aacagcacat cgacatcgcc ctgcgccggc acgccggcat aggtcgacag gatgtcgccg
     3601 gccgggtcgg tgaccggcac ggcatgggcg ggagcggcgg acagcgcggc gagcagcgtg
     3661 gcggcgagcg gcgcgagcag cgaggcgacg ggcggacggc ggtgcatggg gtgttctccc
     3721 tttggtcagg aaccgcgagg ctgcggttcg ccgggggcta caccggcacc ggtgccgggc
     3781 ttccattgcc attcgttcag cttgcccggt cgcgcggatg gcagcgacca gactgcagcg
     3841 accggccggc ccgatccggg gccggccgca gtacggagtc ccgccggtgg ccgaagccgc
     3901 gtccgccccg tccgcatctg ccgccggtgc ccgccgcgcc ggctggttgc gccagtcgcg
     3961 cttctggctg ctgctggccg ctgcgctgct cctcgccggc tcgaccgggc ttgcgctgca
     4021 tcagccgccg cacccggatg cctggcgccc gccaccgggc tggctcgatc cggcgttctg
     4081 gcgctggccg ttcgagcgca acgccttcat gcgtctgccg ctgctcgcgg ccccgatcaa
     4141 cgcgctgcat gtcgccgacg atggcacgac ctggctgctg accggggctg gcgagatcct
     4201 gcacatgtcc ggcgacggcc tgcgctggca gcggcagtgg ccggtggcga cgcaagcgcc
     4261 ggccgcccgc gcggcaggca tcgccgtccc gggcatcgcc gttgcgcacg cccaggccat
     4321 gccaccgctg gacggcgcga tcggcaaggg cagcgaggtg ccgaaccagg ctgcgccgca
     4381 atccccggcc gctccggcct atccgaagcc agggccgacc gcgccggtgc agcagacacc
     4441 gatgacgggc gaagccccgc agccgcgcgc accggcattg aacgccctgc acttcctgag
     4501 cgacgggcgc cacggctggg cggtcggtga cgccggcacg ctgctcgtga ccgacgatgg
     4561 cggcgaacac tggcagcgga tcgaactcgc cgaggccggc gaaaaggtgc tgcgcagcat
     4621 ctggttcgtc gatgcgcaat acggctggat cgtcggcgac gacggcctgc gcctgcgcac
     4681 gatcgacggc ggc
//
LOCUS       DAOQJH010000111         4680 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_10866_length_4680_cov_3.415784, whole genome shotgun sequence.
ACCESSION   DAOQJH010000111 DAOQJH010000000
VERSION     DAOQJH010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 4680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4680
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10866_length_4680_cov_3.415784"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(54..1112)
                     /locus_tag="PLQ18_03780"
     CDS             complement(54..1112)
                     /locus_tag="PLQ18_03780"
                     /inference="COORDINATES: protein motif:HMM:NF014235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HNE00804.1"
                     /translation="MPNRDLLSQDELDALMEGVGNGRVVVGRGQSAGEVLAYDLASPE
                     NVIACIQPALNTVQMRFVHQLGLGFYELLRRDLPLTPVEVHAEPYQDFVARLHPPCSV
                     NVIGLSPMAGPGLILFDHPLVFSLVGAWYGGQGTQYSETDRLEFTAAELRMVQRLNAL
                     CAKALVSAWQPYLPVRWTQHASEISPHLVTAIHPGELLIVFGIDLMLDGTLGTLRVAL
                     PSSMFEPVRGTLLAALAREHPLRDERLQRLLEEGVRDAQVQVSCVLAELEVSVNDLLN
                     LQVGDTIPVDLPDQARLEVDGVRLLAGHFGDTQGWRALQIDRWLVSLPETFELPSAER
                     ALKPVGRDKAPAKAERKP"
     gene            complement(1155..1832)
                     /locus_tag="PLQ18_03785"
     CDS             complement(1155..1832)
                     /locus_tag="PLQ18_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012019019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HNE00805.1"
                     /translation="MPADKLLIGKATAKPCRVGFQIALAGLTFEPSWTAPMAKKEEAK
                     PAEAAAAGGCGGGKGKLIIIILLVLILLILAVGATLFFTGALNKFLGHGGDDGSAVAA
                     GGHGAPMAVPVAVPKSAGPAKYQPLDPPFVVNFDDQGVLRYVQISVSIMARDQKALDK
                     VKENDPQIRYRLILLFSGQTFAELGTPAGKEKLQQKTLEEIQKILIAETGTPGIEAVY
                     FTNFVMQ"
     gene            2069..3121
                     /locus_tag="PLQ18_03790"
     CDS             2069..3121
                     /locus_tag="PLQ18_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005965296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNE00806.1"
                     /translation="MSSTEIAAAAPGRGWGRARRVAAAFVARNIEFVRDRTALGWNLV
                     MPVLIVLGFAFGYSGEPAPQYKIGLLNDGAAHPAFLDTRHVQFVPYAEADTEAAITRV
                     ERHQLDLLIDLRTQHYWVNEESAKGYLAERLLAGSDGGSGFRREAVRGRVIRYVDWLI
                     PGVLSMNMMWSALYGVGYVIVRYRKNGVLKRLKATPLSAVEFLAAQIGSRLTLVLLVS
                     TLVFTGTHLLIGYPMYGSYLHLLLVFALGASSLIGIGLVIAARLRTEEMADGLLNLIS
                     WPMMFLSGIWFSLDGMHPWVQKLALIFPLTHVTTAARAVMIDGAGLATIAPQLGVLAG
                     FTLVCFGIGAALFRWE"
     gene            complement(3129..3479)
                     /locus_tag="PLQ18_03795"
     CDS             complement(3129..3479)
                     /locus_tag="PLQ18_03795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE00807.1"
                     /translation="MPCHWHNTYPPTQQDQAWILPLFQGQTPVAVAEGHHLWVSLRTV
                     VASTQAVDTVTIGAPHVGAFGDQIHVTATLNGVNHHVYYQLATLPVTAPPPHPNLPPM
                     LLGYVQGLLLRVTT"
     gene            3655..4041
                     /locus_tag="PLQ18_03800"
     CDS             3655..4041
                     /locus_tag="PLQ18_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011389789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE00808.1"
                     /translation="MKIAIATKDFRQIAGHAGQSRHWLLYEGTEPGTQPRRIELAPEQ
                     VFHVHRDGVHPLDGIGVVIAGSAGDGFLRRMGKRGVTVALTAESDPARAQRDWLAGCL
                     APPAPPGLRGLICKLRDRLFSAHLRG"
     gene            complement(4052..>4680)
                     /locus_tag="PLQ18_03805"
     CDS             complement(4052..>4680)
                     /locus_tag="PLQ18_03805"
                     /inference="COORDINATES: protein motif:HMM:NF014626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HNE00809.1"
                     /translation="PRYNLSPSQSLTVITEGRGHDRQCHAMRWGLVPGWSKTPSVPYS
                     TINARAETVAEKPVFRSAFRRRRCLIPASGWYEWQPAADGRQPWFMHPRRGQGLLAFG
                     GVWEHWAGDGAEPFDSVAIIVTAAAPSLQPIHDRMPVLIEAPDYEAWLDHENHDAARL
                     QTLMQPADAASVQALRVSTHVNSPKHDDPRCIEPLDAGDRRPAALDAD"
BASE COUNT          673 a         1604 c         1646 g          757 t
ORIGIN      
        1 ggcggcgggg gatgcgctgt cttcggcgcc ttcgggccgg tcctcgggtt cgctcatggc
       61 ttcctctccg ctttggccgg ggccttgtcc cggccgacgg gtttcagggc gcgttcggcg
      121 gatggcagtt cgaatgtttc gggcagcgac accagccagc ggtcgatctg cagcgcccgc
      181 cagccctggg tgtcgccgaa atggccggca agcaggcgca cgccgtcgac ttcgagccgt
      241 gcctgatcgg gcaggtcgac cgggatcgtg tcgccgacct gcaggttcag caggtcgttg
      301 acgctgacct ccagctcggc cagcacgcag ctcacctgca cctgcgcatc gcgcacgccc
      361 tcctccagca gccgctgcag acgctcgtcg cgcagcggat gctcgcgtgc gagcgccgcg
      421 agcagcgtgc cacgcaccgg ctcgaacatc gacgacggca gcgcgacgcg caaggtgccg
      481 agcgtgccgt cgagcatcag gtcgatgccg aacacgatca gcagctcgcc gggatggatg
      541 gccgtgacca gatgcgggct gatctcggag gcgtgctgcg tccagcgcac cggcaggtag
      601 ggctgccagg ccgagaccag cgcctttgcg cacagcgcgt tcaggcgctg caccatgcgc
      661 agctccgcgg ccgtgaactc caggcggtcg gtctcgctgt actgcgtgcc ctgaccgccg
      721 taccaggccc cgaccagcga gaacaccagc gggtgatcga acaggatcag cccgggaccg
      781 gccatcggcg acaggccgat gacgttgacg ctgcacggcg gatgcaggcg cgcgacgaag
      841 tcctggtagg gctcggcgtg cacctcgacc ggcgtcagcg gcaggtcgcg acgcagcagc
      901 tcgtagaagc cgagaccgag ctgatgcacg aagcgcatct gcacggtgtt cagcgccggc
      961 tggatgcagg cgatgacgtt ctcggggctg gcgaggtcgt aggcgagcac ttcgccggcg
     1021 gactggccgc ggccgaccac gacgcggccg ttgccgacgc cttccatcag cgcgtcgagt
     1081 tcgtcctgcg aaagcagatc gcggttcggc acggcggcgg actctccgga acggggcggc
     1141 gatgcaggcg gggcctactg catcacgaag ttggtgaagt acacggcttc gatgccgggc
     1201 gtgccggttt cggcgatcag gatcttctgg atttcctcca gcgtcttctg ctgcagcttt
     1261 tccttgccgg ccggtgtgcc gagttcggcg aaggtctggc cggagaacag caggatcagc
     1321 cggtagcgga tctgcgggtc attctccttg accttgtcga gcgccttctg gtcgcgggcc
     1381 atgatcgaga cgctgatctg cacgtagcgc agcacgccct gatcgtcgaa gttgacgacg
     1441 aaaggcggat cgagcggctg gtacttggcc ggcccggccg acttcggcac cgcgaccggc
     1501 acggccatcg gcgcgccatg gccgccggcc gccaccgcgg aaccatcatc gccgccgtgc
     1561 ccgagaaact tgttgagggc gccggtgaag aacagcgtgg cgcccaccgc caggatcagc
     1621 aggatgagca cgagcaggat gatgatgatc agcttgccct tgccgcctcc gcaaccaccg
     1681 gcggctgccg cttccgcggg cttggcttct tctttctttg ccatgggtgc agtccatgag
     1741 ggttcgaacg tgaggcctgc cagagcaatt tggaagccaa ctcgacaagg tttcgccgtg
     1801 gcctttccaa tcagtagctt gtccgccggc atcacgtcag cgggattccc ggacgcggca
     1861 ccgggccggc agggtgacgg aaatccgtcg ccggatgagc atcagccgcc agcaaccata
     1921 gccgctgtcc ccggcccgcg gcgccccggc gccggctgca atgccgcgca atgcggatgc
     1981 catccgcagc cggcaccgcc gccccggtgg ccgctgtacc cgccgccggc ttacgatccc
     2041 cgcccctgtc ccccgccgga gtcctgacat gtcctcgacc gaaatcgccg ctgccgcgcc
     2101 ggggcgcggc tggggacgcg cgcggcgggt cgcggcggcc ttcgtcgcgc gcaacatcga
     2161 gttcgtgcgc gatcgcaccg cgctcggctg gaatctggtg atgccggtgc tgatcgtgct
     2221 cggcttcgcg ttcggctact ccggcgagcc ggcgccgcag tacaagatcg gcctgctcaa
     2281 tgacggcgcc gcgcacccgg ccttcctcga tacccggcac gtgcagttcg tgccctacgc
     2341 cgaggccgac accgaagccg cgatcacgcg cgtcgagcgc caccagctcg acctgctcat
     2401 cgacctgcgc acgcagcact actgggtcaa cgaggaatcg gcgaagggct atctcgccga
     2461 acggctgctg gccggcagcg acggcggttc cggcttccgg cgcgaggcgg tgcgcggccg
     2521 ggtgatccgc tacgtcgact ggctgatccc cggcgtgctg tcgatgaaca tgatgtggag
     2581 cgcgctgtac ggcgtcggct acgtgatcgt gcgctaccgc aagaacggcg tgctcaagcg
     2641 cctgaaggcg acgccgctga gtgccgtcga attcctcgcc gcgcagatcg gctcgcggct
     2701 gacgctggtg ctgctggtca gcacgctggt gttcaccggc acgcatctgc tcatcggcta
     2761 cccgatgtac ggcagttatc tgcacctgct gctggtgttc gcgctcggcg cgagcagcct
     2821 gatcggcatc ggtctggtga tcgccgcgcg cctgcgcacc gaggagatgg ccgacggcct
     2881 gctgaacctg atctcgtggc cgatgatgtt tttatccggc atctggttct cgctcgacgg
     2941 catgcacccg tgggtgcaga agctcgcgct gatcttcccg ctgacgcacg tcacgaccgc
     3001 ggcgcgcgcg gtgatgatcg acggcgccgg cctcgcgacc atcgcgccgc aactcggcgt
     3061 gctcgccggc ttcacgctgg tctgcttcgg catcggcgcg gcgctgttcc gctgggagtg
     3121 aggcgcgctc aggtggtcac gcgcagcagc aggccctgca cgtagccgag cagcatcggc
     3181 ggcaggttcg ggtgcggcgg cggcgcggtg accggcaggg tcgcaagctg gtagtagacg
     3241 tggtggttga ccccgttgag ggtcgcggtg acgtgtatct ggtcgccgaa ggcgccgacg
     3301 tgcggcgcgc cgatggtcac ggtatcgacc gcctgggtcg atgcgaccac agtgcgcagg
     3361 ctgacccaga gatgatgccc ctcggccacc gcgaccgggg tctgcccctg gaacagcggc
     3421 aggatccagg cctgatcctg ctgggtcggt ggataagtgt tgtgccagtg gcacggcatg
     3481 gtgacagccc cgtgaccggc cccagtgccg gatcatgcga gcttgggtcg ccttcgccga
     3541 gccgccggca agcccggcct tcagggtcct ggcatcgttc ctgctcgaat ccggcaagcg
     3601 ggtcgctcgc ggcccgtccc tgtccgcttg ccgacaagac ctccgcgcat gcccatgaag
     3661 atcgccatcg ccacgaagga tttccgacag atcgccggcc acgccggcca gtcgcgtcac
     3721 tggctgctgt acgaaggcac cgagccgggc acgcagccga ggcgcatcga gctggcgccg
     3781 gagcaggtgt tccatgtgca tcgcgacggc gtgcatccgc tcgacggcat cggcgtcgtg
     3841 attgccggca gtgccggcga cggcttcctg cggcgcatgg gcaagcgcgg cgtcacggtg
     3901 gcgctgaccg ccgaatccga tccggcgcgc gcgcagcgcg actggctggc cggctgcctg
     3961 gcgccgccgg cgccgcccgg cctgcgcggg ctgatctgca agctgcgcga ccggctgttc
     4021 tcggcacacc tgcgcggctg agccgggcct ttcagtcggc gtcgagcgct gccggccggc
     4081 ggtcgccggc gtcgagcggc tcgatgcagc gcggatcgtc gtgcttcggg ctgttgacgt
     4141 gggtgctgac ccgcagcgcc tgcacgctgg cggcatcggc cggctgcatc agcgtctgca
     4201 ggcgcgcggc gtcgtggttc tcgtgatcga gccaggcttc gtaatccggc gcttcgatca
     4261 gcaccggcat gcggtcgtgg atcggctgca gcgacggcgc cgcggccgtg acgatgatcg
     4321 ccacgctgtc gaagggctcg gcaccgtcgc cggcccagtg ctcccagacg ccaccgaagg
     4381 cgagcagccc ctgaccgcga cgcgggtgca tgaaccacgg ctggcgacca tcggcggccg
     4441 gctgccattc gtaccagccg ctggccggga tcaggcagcg gcggcgacgg aaggcgctgc
     4501 ggaacaccgg cttctcggcg accgtctcgg cgcgggcgtt gatcgtgctg tacggcacgc
     4561 tcggcgtctt cgaccagccc ggcaccagcc cccagcgcat cgcgtgacac tggcggtcat
     4621 ggccgcggcc ctcggtgatc acggtcagcg actggctcgg cgacaggttg taacgcggcg
//
LOCUS       DAOQJH010000112         4675 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_10883_length_4675_cov_3.494805, whole genome shotgun sequence.
ACCESSION   DAOQJH010000112 DAOQJH010000000
VERSION     DAOQJH010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 4675)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4675)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4675
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10883_length_4675_cov_3.494805"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            111..416
                     /locus_tag="PLQ18_03810"
     CDS             111..416
                     /locus_tag="PLQ18_03810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE00810.1"
                     /translation="MNSYKGNLSETDAIMKGFHGCVNGNLISRYYEKLAWKATGSLTA
                     DFRKPFAECIGTQGVIRGTSFATNSRTYEQHLKGVMDSCQYIVGIDAFLRILSVLAR"
     gene            1051..1485
                     /locus_tag="PLQ18_03815"
     CDS             1051..1485
                     /locus_tag="PLQ18_03815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE00811.1"
                     /translation="MKKIQALKLTLALLAAGMSMLAPVKLVHAQQYYTRYIYANSVCR
                     YPIRMMVYHKDSENNYHPHGWYNFRPYEESRLKAHNVVLRQVVGYDLYFYAETDNRYN
                     VPAMSWARGDAAVNFEGIYYNLTRANLFVNTKGELEFKLSCA"
     gene            complement(1634..2578)
                     /locus_tag="PLQ18_03820"
     CDS             complement(1634..2578)
                     /locus_tag="PLQ18_03820"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009725807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HNE00812.1"
                     /translation="MSVYTPVDRAQLETFLRDYDIGDYVAHRGISEGISNTNYFLTTT
                     RTEVVLTLFELIGFEDVPYYLDLMAWLAARGVPGANPVADRQGHYMKPLAGKPAALVH
                     KLAGGGLHGTPSLAQCREIGRALAQLHLAGEGFAQRRDNDRGPRWCQSAAERVLPLLD
                     RVEAHLLNDELAALTEHPRSRLPQGTIHADLFRDNALFDGDRLCGLIDFYYACTDALA
                     FDVAVTVNDWCTQPDGTLDRERAAALYGAYAALRPFSEAERAEWPRVLRAAALRFWLS
                     RLVDKLFPTPGELTFIKDPDAFKHILVAHRGAPWRLAD"
     gene            complement(2661..2996)
                     /locus_tag="PLQ18_03825"
     CDS             complement(2661..2996)
                     /locus_tag="PLQ18_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002933162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2782 domain-containing protein"
                     /protein_id="HNE00813.1"
                     /translation="MRSLLLSVLLLAAAGPVALAADEPAPKPPELSPLPESGVDEQPV
                     PDVTITRRAEDTVAEYRINGRLYMIKVTPKKGLPYFLIDSDGDGNLETRHNDLDPGIA
                     IPSWVLLRW"
     gene            complement(3039..3764)
                     /locus_tag="PLQ18_03830"
     CDS             complement(3039..3764)
                     /locus_tag="PLQ18_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018947224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HNE00814.1"
                     /translation="MSPENKQSHPGMAPVNDALLTRESWKIFQIMAEFVEGFERLARI
                     RPSVSIFGSARTQPGHPYYVLAEQIGLLLSDAGFSVVTGGGPGIMEAGNKGAYAGRSP
                     SIGLNIQLPHEQLGNPYQNIALSFRHFFSRKVMFVKYASAYVVLPGGFGTLDEMAEIM
                     TLVQTGKTRRNPIILVHSPFWSPLLAWFRDTLVPEGMIDGGDLELLQLVDDPQQVVDV
                     IFSHYESRGFEPSAEEQEILLDL"
     gene            3906..>4675
                     /locus_tag="PLQ18_03835"
     CDS             3906..>4675
                     /locus_tag="PLQ18_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015281449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HNE00815.1"
                     /translation="MTVLLVDGSSYLYRAFHALPPLSTARGEPTGAVRGVIAMLRNLV
                     TRLAPSRIAVVFDASGPTFRDAIYPAYKAHRPPMPDELRAQIEPLHALVRALGLPLLC
                     EPGVEADDVIGTLARAATAAGESVVIATGDKDLAQLVDARITLLDTMKRDGQETVLDH
                     DGVIAKFGVPPAGIVDLLALMGDSVDNIPGVPGVGPKTAAKWLAEHGSLAALVAAADG
                     IKGKAGEALRAHLAQLDLARQLATIRCDVPLPFGLEALA"
BASE COUNT          780 a         1496 c         1565 g          834 t
ORIGIN      
        1 ggcggctgtg agggcgcgac actttgcact actaggggag cgttgcacag ccctgtgtgc
       61 cgtcccggga cctgggaaag acttgctcat gtgtcggcgt cgtcgcgaac atgaattctt
      121 ataagggtaa cctgtcggaa accgatgcca tcatgaaagg ctttcatggc tgtgtgaatg
      181 gcaatctgat ttcccgttac tatgaaaagc tggcatggaa agccacgggc agtctcacgg
      241 ccgattttcg caaaccgttt gcggagtgca tcgggactca gggggtgatc cgtggtactt
      301 catttgcgac caacagccgg acctatgagc agcatctgaa aggcgtcatg gacagttgtc
      361 agtatattgt cggtatcgac gccttcctgc ggatcctgtc ggttctcgcg cgttgactgc
      421 aggggcaggc cgtaccgtgg ccgggccgcg ctgcccggcc tttggaaaac cgtgaaaatc
      481 cagcagggat cacagatgcc cgagaaatcg tccggctccg gccggacgat ccgtttgcgc
      541 tcggagcagg tctttcggtc atcggcagtc ctgtccggag gcatgaccgg ctgatcgagc
      601 agcccgatcc ctgccaacgg atggtcaggc actggcagag gtacctgtct gccctgcctg
      661 cagcggcttc ggccggcatc gccggtgccc gtgccccgtc cgctgccggt atcccgcctt
      721 ttgtccgtcc gagggtccgt tcccggctgt ttctgccgcc tgatttttgc gcaggtggtc
      781 agggagaggt ccgcagggac gggcaagaaa ggctctgttc agaatgatct gcgcatcctg
      841 gtcaaatccg gaatgcctgt ccgtccaggg aatcagtgct gaacgtcgct gacatgtgac
      901 tccggaactt gatgccggta tgcgacatgg attcatccgc accattggag caggggacac
      961 ggacagggtt gcaggtccgg caggtactac tgcccaagtc taccgaaacg taacagaatt
     1021 tccgcattca tcacgaaagg aatacgcacc atgaagaaga ttcaggctct gaagttgacc
     1081 ctggcactgc tggcagcggg catgagcatg ctggcaccgg tcaagcttgt gcatgctcag
     1141 cagtattaca cccgctacat ctatgcgaac agtgtctgcc ggtatccgat ccgcatgatg
     1201 gtctaccaca aggacagcga aaataactac cacccgcatg gctggtataa tttccgcccc
     1261 tacgaggaaa gccggctgaa agcccataat gtcgtgctgc gtcaggttgt cggctatgat
     1321 ctgtatttct atgctgaaac cgacaatcgt tacaatgtgc cggccatgtc ctgggcacgt
     1381 ggcgatgcgg ccgtcaactt cgaaggtatt tattacaacc tgacgcgtgc caatctgttc
     1441 gtgaacacca aaggcgaact cgaattcaaa ctcagttgcg cctgatgagt gaagcgtata
     1501 ggaacggcag caaccggagt taccggactg agccgcccgc gacgaccggg cggctttttc
     1561 atgccggccc cggcgccgtg tatccggacg cgggccgttt gccgtggact gcggagggac
     1621 ccgggccttg tcttcagtcc gccagccgcc acggcgcgcc gcggtgcgcg acgaggatgt
     1681 gcttgaaggc gtccgggtcc ttgatgaacg tgagttcacc gggcgtcggg aacagcttgt
     1741 cgaccagccg cgacagccag aagcgcagcg ccgcggcgcg cagcacgcgc ggccattcgg
     1801 cccgttcggc ttcgctgaac ggccgaagcg ccgcataggc gccgtacagc gccgcggcgc
     1861 gttcgcgatc cagcgtgccg tcgggttgcg tgcaccagtc gttgacggtg acggccacgt
     1921 cgaaggccag cgcgtcggtg caggcgtagt agaagtcgat cagcccgcag agccggtcgc
     1981 cgtcgaacag tgcgttgtcg cggaacagat cggcgtggat cgtgccctgc ggcaggcgcg
     2041 agcgcgggtg ttcggtcagc gccgcgagtt cgtcgttcag cagatgcgcc tcgacgcgat
     2101 cgagcagcgg cagcacgcgc tcggcggcgc tctggcacca gcgcgggccg cggtcgttgt
     2161 cgcgccgttg cgcgaagccc tcgccggcca gatgcagctg cgcgagcgcg cggccgatct
     2221 cgcggcactg cgcgaggctc ggcgtgccgt gcaggccgcc gccggcgagc ttgtgcacca
     2281 gcgccgccgg cttgccggcc agcggcttca tgtaatgccc ctgacggtcg gcgaccgggt
     2341 tcgcgcccgg cacgccgcgg gccgcgagcc aggccatcag gtcgaggtaa tacggcacat
     2401 cctcgaagcc gatcagctcg aacagcgtca gcacgacctc ggtgcgggtc gtggtcagga
     2461 agtagttggt gttcgagatg ccttcgctga tgccgcgatg cgcgacgtag tcgccgatgt
     2521 cgtagtcgcg gaggaaggtt tcgagctggg cgcgatcgac cggggtgtag acggacatgg
     2581 caggtacttt gcgggcggct ggacagcaag aggccccgta cgcggacggg gcctcgggat
     2641 cggctctcag ggtagcgcgc tcaccagcgc agcagcaccc agctcgggat ggcgatgccg
     2701 ggatcgaggt cgttgtggcg ggtttcgagg ttgccgtcgc cgtcgctgtc gatcaggaag
     2761 tagggcaggc ccttcttcgg cgtcaccttg atcatgtaca gccggccgtt gatgcggtac
     2821 tcggcgacgg tgtcctcggc acggcgcgtg atggtcacgt ccggcaccgg ctgttcgtcg
     2881 acgccgcttt ccggcagcgg gctcagctcg ggcggcttcg gggcgggttc atcggcggcc
     2941 agcgcgaccg ggccggcggc ggcgagcagc agaacggaca gcagcaggct gcgcatcatc
     3001 tctgatcctt ggcgatcggg aaggaagggc gtgggattct acaggtcgag caggatttcc
     3061 tgctcctcgg ccgaaggttc gaagccccgg ctctcgtagt gggaaaatat aacatcgacg
     3121 acctgctgcg gatcgtccac aagttgcagc agttccagat caccgccatc gatcatgccc
     3181 tcgggcacca gcgtgtcgcg aaaccacgcg agcagcggcg accagaacgg gctgtgcacg
     3241 aggatgatcg ggttgcgccg ggtcttgccg gtctgcacca gcgtcatgat ctcggccatc
     3301 tcgtcgagcg tgccgaagcc gcccggcagc acgacgtagg ccgaggcgta cttcacgaac
     3361 atcaccttgc gcgagaagaa gtgccggaag ctcagcgcga tgttctggta cgggttgccg
     3421 agctgctcgt gcggcagctg gatgttcagg ccgatcgacg gcgaacgccc ggcataggcg
     3481 cccttgttgc cggcctccat gatccccggc ccgccgccgg tgacgaccga gaagccggcg
     3541 tccgacagca gcaggccgat ctgctcggcg agcacgtagt acgggtggcc gggctgcgtg
     3601 cgcgccgagc cgaagatgct gaccgagggc cggatgcgtg ccagacgctc gaagccttcc
     3661 acgaactcgg ccatgatctg gaagatcttc cacgactcgc gcgtcagcag cgcgtcgttg
     3721 accggcgcca tgccgggatg ggactgcttg ttctcggggg acatcgcgct gctccggggt
     3781 gattttcgtt tttgatctcg tcgggcgtca ggcttcgccg tgcggccgga ccggccgccg
     3841 tgcgcgcagt ccgcgcccgc ttcctccaac tccagcttat gccgctgcgc gcgagcctcc
     3901 tgcccatgac cgtactgctc gtcgatggct cctcgtatct gtaccgcgcc ttccatgcgc
     3961 tgccgccgct gtcgacggcg cgcggcgagc cgaccggcgc cgtgcgcggc gtcatcgcga
     4021 tgctgcgcaa cctcgtcacg cgcctcgcgc cctcgcgcat cgccgtggtg ttcgacgcca
     4081 gcggcccgac cttccgcgac gcgatctatc ccgcctacaa ggcccaccgt ccgccgatgc
     4141 ccgacgagct gcgcgcgcag atcgaaccgc tgcacgcgct ggtacgcgca ctcggcctgc
     4201 cgctgctctg cgagcccggc gtcgaggccg atgacgtgat cggcacgctg gcgcgcgccg
     4261 ccaccgcggc cggtgagtcc gtcgtcatcg ccaccggcga caaggacctc gcgcagctcg
     4321 tcgatgcgcg catcacgctg ctcgacacga tgaagcgcga cggccaggaa accgtgctcg
     4381 atcacgacgg cgtcatcgcc aagttcggcg tgccgccggc gggcatcgtc gatctgctgg
     4441 cgctgatggg cgattcggtc gacaacatcc ccggcgtgcc cggcgtcggc ccgaagacgg
     4501 ccgcgaagtg gctcgccgag cacggctcgc tcgcggcgct cgtcgccgcg gccgacggca
     4561 tcaagggcaa ggccggcgag gcgctgcgcg cgcatctggc gcagctcgac ctggcccggc
     4621 agctcgcgac catccgctgc gacgtgccgc tgccgttcgg ccttgaagcc ctcgc
//
LOCUS       DAOQJH010000113         4668 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_10910_length_4668_cov_3.222848, whole genome shotgun sequence.
ACCESSION   DAOQJH010000113 DAOQJH010000000
VERSION     DAOQJH010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 4668)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4668)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4668
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10910_length_4668_cov_3.222848"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     repeat_region   1..312
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=1..24
                     /rpt_unit_seq="ccgcggcattacaccgcggaaaac"
     gene            complement(515..814)
                     /gene="cas2"
                     /locus_tag="PLQ18_03840"
     CDS             complement(515..814)
                     /gene="cas2"
                     /locus_tag="PLQ18_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005373083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HNE00816.1"
                     /translation="MSRFQEHLYVVVYDIRDDRRWRRVFRTMKGFGDWVQLSVFQCRL
                     TAVRHAELVARLDGLILHGHDHVLVMDMGPATGIAPRVTSLGLPYEPPEKGPVVL"
     gene            complement(820..2508)
                     /gene="cas1"
                     /locus_tag="PLQ18_03845"
     CDS             complement(820..2508)
                     /gene="cas1"
                     /locus_tag="PLQ18_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004361669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas1"
                     /protein_id="HNE00817.1"
                     /translation="MATDADPTLAAVPPLPARMVNEYVYCPRLAYLMWVQQEWTDSAE
                     TVDGQYVHRQVDRPGGVLPDEPLPEGERIHARSVTLESATLGVIAKLDLVEAEGLRAT
                     PVDYKRGRRPHAARGPHAPERVQLALQGLLLREHGFECREGVIWYAGSRERVPVTLDE
                     DLVTEARSAVAGLRALATAGRLPPPLDDSPKCPRCALVGICLPDETARLRGGDLAPRP
                     LAVADDPAQLLFVQAPYARIGRDGETLTVEIEREPAKPVRLIDVSQLVLHGNVSLSAP
                     ALHELMARDIPVSWHSHGGWFLGHTIGTGHRNVELRSAQYRASFDEAACLRLARGLVE
                     AKIANARTLLRRNWKQGSAPPALLAALKDDIATARTAPSLASLLGVEGAAAARYFGVF
                     AELLNPAPAADGGYSFDFRTRNRRPPADPVNALLSFAYALLTRTLTVTLTAVGFDAYR
                     GFYHQPRYGRPALALDVMEPFRPLIADSAVLQVINNGEVRPRDFVRAGGGVALKPEAR
                     KALVLAFERRLAHEVTHPLFGYRVAYRRLLEIQARLLGRHLLGELPDYPNFTTR"
     gene            complement(2511..3545)
                     /locus_tag="PLQ18_03850"
     CDS             complement(2511..3545)
                     /locus_tag="PLQ18_03850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE00818.1"
                     /translation="MSDTNCRHAETPCPPLALTGLVGSHPLGALAAFGLLRWSAEIPA
                     LRGARLHWELQDDWIAVLTPPAPLDADALCRLLLDAHVANQWPHLDSDELEMPAEDFR
                     ERCLTHLETSNIQDRFTIDFDAAIGCDAIIGKNGNIKTTLLNMTSGNQSFLDKLKSLK
                     KSLIPINRKKHRQEPSRLICSLKEAMFGPWTYSDDEHPLGWDPTMERDHAFRAQAPTN
                     DDKYRSVRFAIWLAHLSLPVLPCIPSGRHLHTMSFAPERYKPPYRFRWPIWQTPIALD
                     SLSALLSSPELTGEDKDVSNLYQRGVSAVYESSRQWRNKGLAIFKHPLLISPAAPQVR
                     RSAPAGPLVF"
     gene            complement(3538..>4668)
                     /locus_tag="PLQ18_03855"
     CDS             complement(3538..>4668)
                     /locus_tag="PLQ18_03855"
                     /inference="COORDINATES: protein motif:HMM:TIGR01596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas3''"
                     /protein_id="HNE00819.1"
                     /translation="TLIVPAAWGGLDVAAGGAWNPALREAVPDLAEAAARRTHGRTVL
                     RLGPACVAQWRLMLRTGADAEAQAAAVAEAAQQLRKLFAEADDNPPDARTVLDELRGI
                     AALPVPVCAALDALAAPGRPPRLLRYGARTQDAELSGWLLAEARPERVAADDGDDASL
                     TVRVGLEAHCRGVGDQASRFAETLGLPPALVDDLGLAGDLHDAGKADPRFQAWLYGGT
                     VPPAAPLLAKSGALDRLDRQAVASARDLAGYPRGGRHECASVQLLRAYPGLLATAHDP
                     ELVEHLVGSHHGRGRPFLPVIDDPGAATLDASFELFDRRLRLRGPHGLERLDSGWPER
                     FARLQRRYGWWGLAWLEALLRLADHRRSEDERRMRQEETDHE"
BASE COUNT          768 a         1547 c         1602 g          751 t
ORIGIN      
        1 ccgcggcatt acaccgcgga aaaccacgat cgtgatgttg cctctgcggg ccacttgcac
       61 gcttcaatga ggccgcggca ttacaccgcg gaaaacgcgg cgaaggtcac gggatcacgc
      121 cccagaaccg caggcttcaa tgaggccgcg gcattacacc gcggaaaacg tgcagtcgat
      181 gccgtcgagg gtcgccggca cgccgcttca atgaggccgc ggcattacac cgcggaaaac
      241 tcgcagcaca aatcgcagca cggatttccc aataaagctt caatgaggcc gcggcattac
      301 accgcggaaa acagaccact cgaaaatccc tttccggaca agtgcttgcg atggggtttg
      361 cgagcgctgt cgggatgggg cgggagggat gttgacttac gtgcggacat ggcggcaagc
      421 gcccttctaa gttgctgatt gacaaagagc ttccggtttg cgagcgctgc ccgggatttt
      481 cgtggcaccg gagcgctcgc gagcactgcg gcagttacag cacgaccggg cctttttccg
      541 gaggttcgta gggcaggccg aggctggtga cgcgcggggc gatgccggtg gccgggccca
      601 tgtccatgac caggacgtga tcatggccat gcaggatcag cccgtccagc cgcgccacca
      661 gctcggcgtg gcgcaccgcc gtcagccggc actggaacac cgacaactgc acccagtcgc
      721 cgaagccctt catggtccgg aagacccgcc gccagcgccg gtcatcgcgg atgtcgtaga
      781 cgaccacata cagatgctcc tggaaccggc tcatcgcgct caccgcgtcg tgaagttcgg
      841 gtaatccggc agttcgccga gcagatgccg gccgagcagc cgtgcctgga tttccagcag
      901 ccggcggtag gcgacgcgat agccgaacag cggatgcgtg acctcgtgcg ccagccgccg
      961 ctcgaaggcc agcaccagcg ccttgcgcgc ctccggcttc agcgccacgc cgccgccggc
     1021 gcgcacgaaa tcgcgcgggc gcacctcgcc gttgttgatg acctgcagca ccgccgaatc
     1081 ggcgatcagc ggccggaacg gctccatcac gtcgagcgcc agcgccgggc ggccgtagcg
     1141 cggctggtga taaaagccgc gataggcatc gaaaccgacc gccgtcagcg tcaccgtcag
     1201 cgtgcgtgtc agcagcgcgt aggcgaacga cagcagcgcg ttgaccggat cggccggcgg
     1261 gcggcggttg cgggtgcgga agtcgaagct gtatccgcca tcggccgccg gtgccggatt
     1321 cagcagttcg gcaaagacgc cgaaatagcg cgccgccgcc gcgccctcga cgccgagcag
     1381 gctggcgagc gacggtgccg tgcgggcggt ggcgatgtcg tccttcagcg ccgcgagcag
     1441 cgccggtggc gcgctgccct gcttccagtt gcgccgcagc agcgtgcggg cattggcaat
     1501 cttggcctcg accagaccgc gcgccagccg caggcaggcg gcctcgtcga agctcgcgcg
     1561 gtactgggcg ctgcgcagtt cgacgttgcg gtgcccggtg ccgatggtgt ggccgaggaa
     1621 ccagccgccg tggctgtgcc agctcaccgg aatgtcgcgc gccatcagct cgtgcagcgc
     1681 cggggcgctg aggctgacgt tgccgtgcag cacgagctgc gagacatcga tcaggcgcac
     1741 cggctttgcc ggctcgcgct cgatctcgac ggtcagcgtc tcgccgtcgc ggccgatgcg
     1801 cgcatacggc gcctgcacga acagcagctg cgccgggtca tcggccaccg ccagcggtcg
     1861 cggcgccaga tcgccgccgc gcagtcgcgc ggtttcgtcg ggcaggcaga tgccgaccag
     1921 cgcgcagcgc gggcacttcg gactgtcgtc gagcggcggc ggcagccggc cggccgtggc
     1981 cagcgcgcgc aggccggcga ccgcgctgcg cgcttcggtg acgaggtcct catcgagcgt
     2041 caccggcacg cgctcgcggc tgccggcgta ccagatcacg ccttcccggc actcgaagcc
     2101 gtgctcgcgc agcagcaggc cctgcagcgc gagctgcacg cgctcgggtg catgcgggcc
     2161 gcgcgccgcg tgcgggcgcc tgccgcgctt gtagtcgacc ggtgtggcgc gcagaccttc
     2221 ggcctcgacc agatcgagct tcgcgatgac gccgagcgtg gcggattcga gcgtcaccga
     2281 gcgcgcatgg atgcgctcgc cttcgggcag gggctcatcg ggcagcacgc cgccgggtcg
     2341 atcgacctgc cggtgcacgt actggccgtc gacggtctcg gcgctgtcgg tccattcctg
     2401 ctgcacccac atcaggtacg cgagccgcgg gcagtacacg tactcgttga ccatgcgcgc
     2461 cggcagcggc ggcacggcgg cgagcgtggg gtcggcgtcg gtcgccatgt tcagaacacc
     2521 agcggaccgg ccggtgcgct gcgacggacc tgcggagcgg cgggagaaat gagcaaagga
     2581 tgtttgaata tggcaagtcc tttattacgc cattgacgtg acgattcata aacagcagat
     2641 acaccgcgct gatacaggtt gctgacatcc ttgtcttctc cggtcaattc tggtgaagat
     2701 agcaatgcgc ttaatgaatc cagtgctatt ggggtttgcc agattggcca tctgaatcgg
     2761 tacggtggct tgtatctttc tggtgcgaaa gacattgtgt gcaaatggcg acctgatggg
     2821 atgcatggaa gcaccggaag tgacaagtgt gccaaccaaa tggcaaacct gactgatcga
     2881 tacttgtcgt cattggttgg cgcttgtgcg cggaatgcat ggtcccgctc catagtggga
     2941 tcccagccaa gcgggtgctc atcgtcagaa tatgtccatg gcccgaacat ggcttctttt
     3001 aatgagcaga tcagacgcga aggttcttgg cgatgttttt ttctatttat agggatcaat
     3061 gattttttta gagattttaa tttatccaaa aaactctgat tgccggatgt catgttgagc
     3121 agggttgttt tgatattgcc gtttttgccg attatggcat cgcagccaat agcggcatca
     3181 aaatcgattg tgaatctatc ttgaatgttt gatgtttcca aatgggtcaa gcatcgctct
     3241 ctgaaatctt cggcaggcat ttccagttcg tcgctgtcca aatgaggcca ttgattggcg
     3301 acatgcgcat cgagcagcag ccggcacagc gcatcggcat cgagcggcgc cggcggtgtc
     3361 agcaccgcga tccagtcatc ctgcagctcc cagtgcagcc gcgccccgcg cagcgccgga
     3421 atctccgcgc tccagcgcag cagcccgaag gctgccagcg caccgagcgg atgactgccg
     3481 acgaggccgg tcagcgcgag cggcgggcag ggggtttctg cgtgccggca gttcgtatca
     3541 ctcatgatcc gtttcctcct gccgcatccg gcgctcgtcc tcggagcgcc ggtggtcggc
     3601 cagccgcagc agggcttcga gccaggcgag tccccaccag ccgtaacgcc gctgcaggcg
     3661 cgcgaagcgt tccggccagc cgctgtcgag ccgctccagc ccgtgcggtc cgcgcagccg
     3721 caagcgccgg tcgaacagct cgaagctggc gtcgagcgtt gcggcgccgg ggtcgtcgat
     3781 gaccggcagg aacggccggc cgcggccgtg gtgactgccg accagatgct cgaccagctc
     3841 cgggtcgtgg gcggtggcga gcaggccggg gtaggcgcgc agcagctgca ccgacgcgca
     3901 ttcgtgccgg ccgccgcgcg gatagccggc cagatcgcgg gcgctggcga cggcctgacg
     3961 gtcgagccgg tcgagtgcgc cggacttggc cagcagcggc gcggcgggcg gcaccgtgcc
     4021 gccgtacagc caggcctgga agcgcggatc ggccttgccg gcgtcgtgca ggtcaccggc
     4081 cagaccgagg tcatcgacca gcgccggcgg caggccgagc gtttcggcga agcggctggc
     4141 ctgatcgccg acgccgcggc agtgcgcctc cagcccgacg cgcacggtca gcgaggcgtc
     4201 gtcgccatcg tcggccgcga cccgctccgg ccgcgcctcg gccagcagcc agccgctcag
     4261 ctcggcgtct tgggtgcggg cgccgtagcg cagcagccgc ggcggcctgc ccggcgcggc
     4321 cagcgcatcg agtgccgcgc acaccggcac cggcagggcg gcgatcccgc gcagttcatc
     4381 gagcaccgtg cgggcgtccg gcgggttgtc gtcggcctcg gcgaagagct tgcgcaactg
     4441 ctgcgcggct tcggccaccg cggcggcctg tgcctcggca tccgccccgg tccgcagcat
     4501 cagccgccac tgcgcgacgc aggccggacc gaggcgcagc acggtgcggc cgtgtgtccg
     4561 gcgcgctgcc gcttcggcca gatccggcac cgcctcgcgc agcgccgggt tccaggcgcc
     4621 gccggcggcg acatcgagcc cgccccaggc tgccggcacg atcagcgt
//
LOCUS       DAOQJH010000114         4650 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_10975_length_4650_cov_3.856148, whole genome shotgun sequence.
ACCESSION   DAOQJH010000114 DAOQJH010000000
VERSION     DAOQJH010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 4650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4650
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10975_length_4650_cov_3.856148"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..522)
                     /gene="rfbC"
                     /locus_tag="PLQ18_03860"
     CDS             complement(<1..522)
                     /gene="rfbC"
                     /locus_tag="PLQ18_03860"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015004309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HNE00820.1"
                     /translation="MKLIETALPGVLILEPQVHGDARGFFVETYRASWFEAHGLTAPV
                     FVQDNQSRSRRGVLRGLHYQTVNPQGKLVRCARGAVFDVAVDVRCGSPHFGQSVGVVL
                     DDESHRQIWIPPGFAHGFCVLSDVADFAYKCTSYYDPASDVGIRWNDPALDIGWPLED
                     IEPLLSAKDQLLPL"
     gene            complement(519..1403)
                     /gene="rfbA"
                     /locus_tag="PLQ18_03865"
     CDS             complement(519..1403)
                     /gene="rfbA"
                     /locus_tag="PLQ18_03865"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HNE00821.1"
                     /translation="MDRKGLILAGGSGTRLHPLTLSVSKQLMPVYDKPMIYYPLTTLM
                     LAGIREILIITTPRDQEAFQALLGDGSQWGVRLEYAVQPSPDGLAQAFLIGEEFLAGS
                     PAALVLGDNIYHGHGLPELLRRADAREQGATVFGYYVRDPERYGVVEFDAAGRAVSVE
                     EKPAQPKSNYAITGLYFYDADVVEVAKVVRPSARGELEITDVNRIYLERGQLNVELMG
                     RGYAWLDTGTHDSLLDAANFIQVMQARQGLQIACPEEIAWRLGWIDAEHLERMAKPLA
                     KNGYGRYLLDLLRQGVRP"
     gene            complement(1486..3198)
                     /locus_tag="PLQ18_03870"
     CDS             complement(1486..3198)
                     /locus_tag="PLQ18_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018718525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional sulfate
                     adenylyltransferase/adenylylsulfate kinase"
                     /protein_id="HNE00822.1"
                     /translation="MTTLIAPHGGTLKNLYLTPELAAAEKQKAKELLSWDLDHRQLCD
                     IELLLNGAFSPLEGFLGRADYDRVVSEMRLADGTLWPMPIMLDVSEAFAAQVQVGATI
                     ALRDPEGVLIATMEIADLWTPDKALEAQNVFGTTDEKHPGVAYLMRKAGPVYIGGKLK
                     GVEEPMHYDFRSLRNSPAEMRALFNKLGWRKIVAFQTRNPMHRAHQELTFRAAKLAQA
                     NLLIHPVVGMTKPGDIDHYSRVRCYEHLIKQYPEQSTMLSLLHLAMRMGGPREAVWHA
                     IIRKNHGCTHFIVGRDHAGPGKDSNGKDFYGPYDAQEMVAQYAEELGIEMVPFQEMVY
                     VEELAEYVPQDEVKPGQTVLNISGTEFRRRLMEGLEIPAWFSYPEVVAELRRTHPPKH
                     LQGFTVFFTGLSGSGKSTIANALMVKLMELGGRRVTMLDGDLVRKNLSSELGFSKEHR
                     NINILRIGYVASEITKNGGIAICAPIAPYTQTRKDVRDMIAPLGGFIEIHVATTVETC
                     EQRDRKGLYAKARAGIIKEFTGISDPYEAPENPEMKIQTEECTPDEAAQRILLKLESL
                     GYIR"
     gene            complement(3342..>4650)
                     /locus_tag="PLQ18_03875"
     CDS             complement(3342..>4650)
                     /locus_tag="PLQ18_03875"
                     /inference="COORDINATES: protein motif:HMM:NF015557.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HNE00823.1"
                     /translation="STNLVVNGLIARQGLAPLGMFDPMWVGLPCVLVGGLYLMTVGRW
                     LLPARRSAVEQVANAREYTLEMIVEEQGPLVGLSVEEAGLRALPQLYLIEIVREGRVV
                     QAIAPQEILRADDRLVFVGIVDSVLDLRRMRGLRPATDQIFKLAVRHNQRCLIEAVVS
                     PVSPCVGRTIRESRFRNRYNGAVIAVSRRGERVHRKLGDIVLQAGDTLLIEAGAQFAR
                     HHRYARDFLLVSAVDESTATDHSRAPIALGILAALVIGTTVFDVDTLYGALLAAAAMI
                     ATRCVNQGQARRSIDYQLIVIIACSFALGLAIEKTQVAKLVAQLMMLSVSNDPWGTLA
                     VIYVLTVLATELLTNNAAAVLMFPIGVAAARQLGVSELPFVMSVMVGASAGFISPIGY
                     QTHLMVMGPGGYRFSDFFRIGTPLSVLVGLTTIVVAPLAWPFR"
BASE COUNT          899 a         1491 c         1430 g          830 t
ORIGIN      
        1 cagcggcagc agttgatcct tggccgacag caacggttcg atgtcctcaa gcggccagcc
       61 gatgtcgagc gccgggtcgt tccagcggat gccgacatcg gaggccgggt cgtagtaact
      121 ggtgcatttg tacgcgaagt cagccacgtc gctgagcacg cagaaaccgt gcgcaaagcc
      181 gggcggaatc caaatctggc gatgcgattc atcatcgagc acgacgccca cggactggcc
      241 gaaatgcggc gaaccgcagc gcacgtccac ggcgacatcg aacaccgcgc cgcgcgcgca
      301 gcgcaccagc ttgccctgcg gattgacggt ctggtaatgc aggccgcgca acacgccgcg
      361 gcgcgagcgg gactggttgt cctgcacaaa cacgggtgcc gtcaggccat gcgcctcgaa
      421 ccagctggcg cggtaggtct cgacgaaaaa gccgcgggca tcaccatgca cctgcggttc
      481 gaggatcagt acgcccggca gtgcggtctc gatcagtttc atgggcgcac accctgtcgc
      541 agcagatcga gcaggtagcg gccgtagccg ttcttggcga gcggttttgc catgcgctcg
      601 aggtgttcgg cgtcgatcca gccgaggcgc caggcgattt cttcggggca ggcgatctgc
      661 agtccctgac gggcctgcat gacctgaatg aagttcgccg catcgagcag cgaatcatgc
      721 gtgccggtgt cgagccacgc ataaccgcgc cccatcagct cgacgttgag ttgcccgcgc
      781 tccaggtaga tacggttgac atcggtgatc tccagttcac ctcgggccga aggtctgacg
      841 acttttgcca cctcgaccac atcagcatcg tagaagtaca ggccggtgat cgcgtaattc
      901 gacttcggct gcgccggttt ttcctcgacc gacaccgcac gcccggctgc atcgaactcg
      961 accacgccgt agcgctcggg atcgcgcacg tagtagccga acaccgtcgc gccctgctcc
     1021 cgtgcatcgg cccggcgcag cagttccggc aggccgtggc cgtgatagat gttgtcgccc
     1081 agcaccagcg ccgccggact gccggcgagg aattcctcgc cgatcaggaa ggcctgcgcg
     1141 aggccatccg gactcggctg cacggcgtat tccagccgca cgccccactg gctgccgtcg
     1201 ccgagcagcg cctggaatgc ctcctggtcg cgcggcgtcg tgatgatcag gatctcccgg
     1261 atgccggcga gcatcagcgt cgtcagcggg tagtagatca tcggcttgtc gtacaccggc
     1321 atcagctgct tgctcaccga caacgtcagc ggatgcagcc gcgtgccgga gccgccagcg
     1381 aggatgaggc ctttgcgatc catgaggaat ctctcgaata aactcggact tcaggaagga
     1441 agaaagaact tctccttccc gtgcggcatt gccctcaatg cgaggtcagc ggatgtagcc
     1501 gaggctttcc agcttcagca ggatgcgctg cgcagcctcg tcgggcgtgc actcctcggt
     1561 ctgaatcttc atctcgggat tctccggcgc ttcgtacgga tcggaaatac cggtgaattc
     1621 cttgatgatg ccggcgcgag ccttggcata cagacccttg cgatcacgct gctcgcaggt
     1681 ttcgaccgtg gtcgcaacgt ggatttcgat gaagccgccg agcggcgcga tcatgtcgcg
     1741 cacatccttg cgggtctgcg tatagggtgc gatcggcgca cagatcgcga tgccaccgtt
     1801 cttggtgatc tcgctggcga catagccgat gcgcaggata ttgatgttgc ggtgctcctt
     1861 gctgaacccg agttccgaag acaggttctt gcgcaccaga tcgccgtcca gcatcgtcac
     1921 ccgccggccg ccgagttcca tcagcttgac catcagggcg ttggcaatgg tcgacttgcc
     1981 ggaaccggac agccccgtga agaacaccgt gaagccctgc aggtgcttcg gcggatgcgt
     2041 acggcgcagt tcggcgacca cttccgggta cgagaaccag gccggaattt ccagcccttc
     2101 catcagacgg cgacggaact cggtgccgga aatgttcagc acggtttgac ccggcttgac
     2161 ctcgtcctgc ggaacatact cggccagttc ctcgacgtag accatctcct ggaacggcac
     2221 catctcgata ccgagttctt cggcgtactg cgccaccatt tcctgcgcgt cgtacggacc
     2281 gtagaaatcc ttgccgttcg aatccttgcc cggaccggca tggtcgcgac cgacgatgaa
     2341 atgcgtgcag ccgtgattct tgcggatgat cgcgtgccag acggcctccc gcggtccgcc
     2401 catgcgcatc gccagatgca gcagcgacag catcgtgctc tgctccggat actgcttgat
     2461 caggtgctcg tagcagcgca cgcgcgaata atggtcgata tcaccgggct tggtcatgcc
     2521 gacgacgggg tgtatcagca ggttggcctg ggcaagcttg gccgcgcgga aggtcagttc
     2581 ctgatgcgcg cgatgcatcg gattgcgcgt ctggaacgca acgatcttgc gccagccgag
     2641 cttgttgaac agcgcccgca tttcggccgg cgagttgcgc aggctgcgga agtcgtagtg
     2701 catcggctct tcgacgccct tgagcttgcc accgatgtag accgggccgg ccttgcgcat
     2761 caggtaggcg acacccgggt gtttctcgtc ggtcgtgccg aacacgttct gcgcctcaag
     2821 cgccttgtcc ggcgtccaca gatcggcaat ctccatcgtc gcgatcagca cgccctcggg
     2881 atcgcgcaat gcgatcgtcg cgccgacctg aacctgggcc gcaaacgcct cgctgacatc
     2941 gagcatgatc ggcatcggcc acagcgtacc gtcggccagc cgcatttcgc tgaccacgcg
     3001 gtcatagtcg gcacgcccga gaaagccctc aagcggcgaa aacgcaccgt taagcagcag
     3061 ttcgatgtca cagagctggc ggtggtccag atcccaggac agcagttcct tcgccttctg
     3121 cttctcggct gcggccagtt cgggcgtcag atacaggttc ttgagggtgc cgccatgggg
     3181 ggcaatcagc gtcgtcatgc gatcaggctt ccatcaggtc gttgaatcgg gagcgccgca
     3241 cagcggcgct actgcaatgc agcatacccc atgagccccg gcggacagaa caacaggggc
     3301 agcggtatgc acgggtcggc cacacatgtt gtgcatgtcg ttcagcggaa cggccatgcg
     3361 agcggcgcga cgacgattgt ggtcagacca accagcacgc tgagcggcgt gccgatccgg
     3421 aagaaatccg agaagcgata accgcccggc cccatgacca tcagatgtgt ctggtagccg
     3481 atcggcgaga tgaaaccagc cgaggcgccg accatcacgc tcatgacgaa cggcagttcg
     3541 ctgacaccga gctggcgtgc tgcagccacg ccgatcggaa acatcagcac ggctgcggca
     3601 ttgttggtca gcagctcggt cgccagcacc gtcagcacgt agatcaccgc gagcgtgccc
     3661 cagggatcgt tgctgaccga caacatcatc agctgtgcaa ccagctttgc aacctgggtc
     3721 ttctcgatcg ccagtccgag cgcgaacgag caggcaatga tgacgatgag ctggtaatcg
     3781 atcgaacgac gtgcctggcc ctgattgacg caacgcgtcg cgatcatcgc tgcggctgcc
     3841 agcagggcac catagagggt atcgacgtca aatacggtcg tgccgatcac cagagccgcg
     3901 agaatgccga gtgcaattgg tgcccgtgaa tgatcggtgg cggtcgattc atcgacagcg
     3961 ctgaccagca gaaagtcgcg tgcatagcga tggtgccgcg caaactgggc acctgcctcg
     4021 atcagcagcg tatcgccggc ctgcagcacg atgtcgccga gtttgcgatg cacgcgttcg
     4081 ccgcgccgtg aaaccgcgat gaccgcgccg ttgtagcgat tgcgaaaacg ggattcccgg
     4141 atcgtgcgtc cgacacaggg tgaaaccggc gagaccacgg cttcgatcag gcaacgctga
     4201 ttatggcgga cggccagttt gaagatctgg tcggttgccg ggcgcaagcc acgcatgcgg
     4261 cgcagatcaa gcaccgaatc cacgatgccg acgaaaacca gccggtcatc cgcgcgcagg
     4321 atttcctgcg gggcaatcgc ctgcaccacc ctgccctcac gcacgatctc gatcagatag
     4381 agctgcggca gcgcgcgcag gccggcctct tcgacgctca gcccgaccag cggtccctgc
     4441 tcctcgacga tcatttcgag cgtgtactca cgcgcattgg caacctgctc gactgccgag
     4501 cgtcgggccg gcagcagcca gcgaccgacc gtcatcagat aaaggccgcc gaccagcaca
     4561 cacggcaggc ccacccacat cggatcgaac atgcccagag gtgcaagtcc ttgccgtgca
     4621 atcaaaccat tgacaacgag attcgtactc
//
LOCUS       DAOQJH010000115         4633 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_11030_length_4633_cov_3.737221, whole genome shotgun sequence.
ACCESSION   DAOQJH010000115 DAOQJH010000000
VERSION     DAOQJH010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 4633)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4633)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4633
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11030_length_4633_cov_3.737221"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            87..740
                     /locus_tag="PLQ18_03880"
     CDS             87..740
                     /locus_tag="PLQ18_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide amidohydrolase"
                     /protein_id="HNE00824.1"
                     /translation="MDQAVGLDVSCEPPWLQVDLGAPHRCLGWAVHRPGRVVARHVVW
                     LQVDEAELAPGVDPAALLGARLAARGLARAVGLMTARRVSAWEAGRAVVGDVGVDTVL
                     TLGLSNAVHVAGEAGPQPWRPGTINLLARLSVPLTDAALVEAVSIAAEARTAALLETA
                     GPAGGPLTGTGTDCIVIAVPPGPGRPYAGLHTATGQALGRAVYDTTRRAALAWQAQR"
     gene            complement(749..961)
                     /locus_tag="PLQ18_03885"
     CDS             complement(749..961)
                     /locus_tag="PLQ18_03885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE00825.1"
                     /translation="MHTLSTEQLLTWIRWYRRHRRTALARIPEDVPGTSGPDAAPTCE
                     PACEPACEPADATPAVTRRQAGALKR"
     gene            complement(1157..3328)
                     /gene="recQ"
                     /locus_tag="PLQ18_03890"
     CDS             complement(1157..3328)
                     /gene="recQ"
                     /locus_tag="PLQ18_03890"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020484957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HNE00826.1"
                     /translation="MSDPALRILRTVFGYDAFRGAQAEIVAHVAGGGDALVLMPTGGG
                     KSLCYQIPALLRAGTGLVVSPLIALMHDQVDALTQLGVRAAYLNSSLDAASVRETERA
                     FAAGELDLLYVAPERLVTERCLGLLARTPLALFAIDEAHCVSQWGHDFRPEYRALTVL
                     HERFPDVPRVALTATADALTRDDIREHLGLLDARVFVSSFDRPNIRYRIVAKDRAREQ
                     VLAFIRAEHAGEAGIIYCLSRRSVDETAAWLAGRGIHALPYHAGLEAGVRRDHQQRFL
                     REEGVVMVATVAFGMGIDKPNVRFVAHLDLPKSVEGYYQETGRAGRDGLPADAWLSYG
                     LNDAIQLRRMLAESGADERQKRVESLKLDSLLAYCETPRCRRQVLLAYFGEQYPGPCG
                     NCDTCLSPVETWDATVAAQKALSCVHRTGARFGVAHLIDVLLGKATDKVAQHGHDKVS
                     TFAIGSELGEAQWRSVYRQLVAAGLLAVDLEGYGALKLTNAARPVLRGETALHLRKDP
                     PPNRRTREREPATGGPRRESPAQTLSAEQRVLWDALRQKRLELAREQGVPPYVIFHDA
                     TLLDFIVRAPRTLAELATVSGVGRSKLERYGETFLAVLLGHEAGEAPAADAEAGDPAA
                     QARSLLLRGAGVDEIAARLDVPRSQVLGWLAPLVRRGEAELDEVLPQLSVAELETIEA
                     AFRSVDPAGWHDLKPIHAALGERHDFGTLACIRAALRRPDA"
     gene            complement(3393..4037)
                     /locus_tag="PLQ18_03895"
     CDS             complement(3393..4037)
                     /locus_tag="PLQ18_03895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE00827.1"
                     /translation="MAAVHDFQHDPGFAEQQDALSTLLESLLSPEGVRAALTVWSRDY
                     AHRPGALRAYANCLVREFGVPLGLREMHRQLVQHLLLPATRRGPVLLAAAAQTPDRAV
                     PALTRAFVLLTGALLARCGQQDPKAATALRSTLVGAAMTAKLSRADRELLVAWLDGSG
                     PVPARAYDEALLRELVHAAWAGLCTLWGPPVADRQLGAAVHAAEEQCAEVRRLL"
     gene            complement(4039..>4633)
                     /locus_tag="PLQ18_03900"
     CDS             complement(4039..>4633)
                     /locus_tag="PLQ18_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018953643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HNE00828.1"
                     /translation="GWFGFNGGSTAVAEAGIGRIVLNTHFGAAAGAIGTIAMQRLLQA
                     PVLLTATVNGSIAGLVSVTAGCATMEPLHAMFTGLIAGMLMVASERAMLHLRLDDVVG
                     AVPVHGVCGAWGTLAAGLFYAPHLFDPTRVTVQVIGICTAFIWAFPSAWLMFKAIDLT
                     IGLRARGIAEQRGLDYAEHHEQGYPEFQGIALHEEIR"
BASE COUNT          620 a         1597 c         1792 g          624 t
ORIGIN      
        1 gtgccgggag tgtgtgcggt cggccgttcc gtgacaatgg cgcggccgca ataccgcgga
       61 gcgcaggcgc agggggcgag gccggcatgg atcaggccgt cggactggac gtgagctgcg
      121 agccgccgtg gctgcaggtc gatctcgggg cgccgcaccg ctgtctgggc tgggccgtgc
      181 accggccggg ccgcgtcgtc gcccggcacg tggtctggct gcaggtcgac gaggcggaac
      241 tcgcccccgg cgtcgatccg gcggcgctgc tgggtgcgcg gctggccgcg cgcgggctgg
      301 cgcgggccgt cggcctgatg actgcgcgcc gggtcagcgc ctgggaggcc gggcgggcgg
      361 tggtcgggga tgtgggggtc gatacggtgc tgacgctcgg tctgtcgaac gcggtccacg
      421 tcgccggcga ggccggcccg cagccgtggc ggccgggcac gatcaatctg ctggcgcggc
      481 tgtcggtgcc gctcaccgac gcggcgctgg tcgaggcggt gtcgatcgcc gccgaggcgc
      541 gcaccgcggc gctgctcgaa accgccggtc ccgccggcgg cccgctgacc ggcaccggca
      601 ccgactgcat cgtcatcgcc gtgccgccgg gccccggccg gccgtacgcc ggcctgcaca
      661 ccgcgaccgg tcaggcgctg ggccgggcgg tctacgacac cacccggcgc gcggcgctgg
      721 cctggcaggc gcagcgctga ggtggcgctc agcgcttcaa agcgccggcc tggcggcggg
      781 tcacggcggg ggttgcgtcg gccggttcgc aggccggttc gcaggccggt tcgcaggtcg
      841 gcgccgcgtc gggtccggac gtgccgggca cgtcctcggg aatgcgcgcc aaggccgtgc
      901 ggcgatgacg ccgataccag cggatccagg tcagcagctg ttccgtgctc agggtgtgca
      961 tccgttgctc cttgcgggaa atggaatctt cggggtggga ggcgggcggg ggacgagagg
     1021 gatcgggcac ggcggaccgg gacgcacagg gtagccgatg gcagtcgcgg cgggcgatgc
     1081 aggaggtgca cggctgctga tgaatcagat cacggtgcga tcctgcgttc tcgggctgcg
     1141 ggccttgccg gatgattcag gcgtccgggc ggcgcagggc ggcgcggatg caggcgagcg
     1201 tgccgaaatc gtggcgttcg ccgagcgcgg cgtggatcgg cttcaggtcg tgccagccgg
     1261 ccggatcgac gctgcggaag gcggcctcga tggtttcgag ctcggcgacc gacagctgcg
     1321 gcagcacctc gtcgagttcg gcctcgccgc ggcgcaccag cggggccagc cagccgagca
     1381 cctggctgcg cggcacatcg aggcgcgcgg cgatctcgtc gacgccggca ccgcgcagca
     1441 gcaggctgcg ggcctgggcg gccggatcgc cggcctcggc gtcggcagcc ggggcctcgc
     1501 cggcctcgtg tccgagcagc acggccagga aggtctcgcc gtagcgttcg agcttgctgc
     1561 gcccgacgcc ggagacggtc gccagttcgg cgagcgtgcg cggtgcgcgg acgatgaaat
     1621 cgagcagcgt cgcatcgtgg aagatcacgt acggcggcac gccctgctcg cgcgccagtt
     1681 cgaggcgctt ctggcgcagc gcgtcccaca gcacgcgctg ctctgctgac agcgtctgcg
     1741 ccgggctttc gcggcgcggt ccgccggtgg ccggttcgcg ttcgcgcgtg cgccggttcg
     1801 gcggcgggtc cttgcgcagg tgcagcgccg tctcgccgcg cagcaccggt cgcgcggcgt
     1861 tcgtcagctt cagcgcaccg tagccttcga gatcgaccgc gagcaggccg gcggcgacca
     1921 gctgccggta gaccgagcgc cactgcgcct cgcccagttc gctgccgatg gcgaaggtgc
     1981 tgaccttgtc gtggccgtgc tgcgcgacct tgtcggtggc tttgccgagc agcacgtcga
     2041 tcaggtgcgc gacgccgaag cgcgcgccgg tgcggtgcac gcaggacagc gccttctgcg
     2101 cggcgacggt cgcatcccag gtctcgaccg gcgacaggca ggtgtcgcag ttgccgcagg
     2161 gtccggggta ttgctcgccg aagtacgcga gcagcacctg ccggcggcag cgcggggtct
     2221 cgcagtaggc gagcaggctg tcgagcttca gcgattcgac gcgcttctgg cgctcgtcgg
     2281 cgccggattc ggcgagcatg cgccgcagct ggatcgcgtc attgagcccg tagctgagcc
     2341 aggcgtcggc cggcaggccg tcgcggccgg cgcggccggt ctcctggtaa tagccctcga
     2401 ccgacttcgg caggtcgagg tgcgcgacga agcgcacgtt cggcttgtcg atgcccatgc
     2461 cgaaggcgac ggtcgcgacc atgaccacgc cttcctcccg taaaaaacgc tgctggtgat
     2521 cgcggcgcac cccggcctcc agcccggcgt ggtagggcag ggcgtggatg ccgcgtccgg
     2581 ccagccaggc ggcggtttcg tcgacgctcc tgcgcgacag gcagtaaatg atgccggcct
     2641 cgccggcatg ctcggcccgg atgaaggcga gcacctgctc gcgggcgcgg tccttggcga
     2701 cgatgcggta gcggatgttc ggccggtcga agctcgacac gaacacgcgc gcatcgagca
     2761 ggccgaggtg ctcgcggatg tcgtcgcgcg tcagggcgtc ggcggtcgcg gtcagcgcca
     2821 cgcgcggcac gtccgggaag cgctcgtgca gcaccgtcag cgcccggtac tcgggccgga
     2881 agtcgtggcc ccactgcgac acgcagtgcg cctcgtcgat cgcgaacagc gcgagcggcg
     2941 tgcgcgcgag caggccgagg cagcgctcgg tgaccagccg ctcgggcgcg acgtagagca
     3001 ggtcgagttc gccggcggcg aatgcgcgct cggtttcgcg cacgctggcg gcatcgaggc
     3061 tggagttcag gtacgccgcg cgcacgccga gctgggtcag cgcatcgacc tgatcgtgca
     3121 tcagcgcgat cagcggcgag acgacgaggc cggtgccggc gcgcagcagc gccgggatct
     3181 ggtagcacag cgacttgccg ccgccggtcg gcatcagcac cagcgcatcc ccgccgccgg
     3241 cgacgtgcgc gacgatctcg gcctgcgcgc cgcggaaggc gtcgtagccg aagacggtgc
     3301 gcaggattct cagggcggga tcggacatgg gggcgcaagg atacgcggac ccgccacggc
     3361 tgccacgtgc gccggctcgc gcccgccggc gttcagagca ggcgccggac ctcggcgcac
     3421 tgctcctcgg cggcatgcac ggccgcgccg agctggcggt cggcgaccgg cggaccccag
     3481 agcgtgcaga gcccggccca ggccgcatgc acgagttcgc gcagcagggc ttcgtcatag
     3541 gcccgcgccg gcaccgggcc gctgccgtcc agccacgcga ccagcagctc gcgatcggcg
     3601 cgcgagagct tcgccgtcat cgccgcgccg accagcgtac tgcgcagtgc cgtcgcggcc
     3661 ttcgggtcct gctgcccgca gcgcgcgagc agtgcaccgg tcagcagcac gaaggcgcgc
     3721 gtcagcgccg gcaccgcccg gtccggggtt tgcgccgccg cggccagcag caccggaccg
     3781 cggcgcgttg ccggcagcag caggtgctgc acgagctggc ggtgcatctc gcgcaggccg
     3841 agcggcacgc cgaactcgcg caccaggcag ttcgcgtagg cgcgcagcgc cccgggccgg
     3901 tgcgcgtagt cgcgcgacca gacggtgagc gcggcgcgca cgccttcggg ggacagcagg
     3961 ctttcgagca gcgtcgacag ggcgtcctgc tgctcggcga agcccgggtc atgctggaaa
     4021 tcgtgtacgg cagccatgtc agcggatctc ctcgtgcagg gcgatgccct ggaattccgg
     4081 atagccctgt tcgtgatgtt cggcgtaatc gagaccgcgc tgctcggcga tgccgcgcgc
     4141 gcgcaggccg atcgtcaggt cgatggcctt gaacatcagc caggcgctcg ggaaggccca
     4201 gatgaaggcc gtgcagatgc cgatgacctg cacggtgacg cgggtcggat cgaacaggtg
     4261 cggggcatag aacagaccgg cggcgagcgt gccccaggcg ccgcagacgc cgtgtaccgg
     4321 cacggcaccg accacgtcgt cgagccgcag gtgcagcatc gcccgttcgg aggcgaccat
     4381 cagcatcccg gcgatcagac cggtgaacat cgcatgcagc ggctccatgg tcgcgcagcc
     4441 ggccgtcacc gacaccaggc cggcgatcga gccgttgacg gtcgccgtca gcagcaccgg
     4501 ggcctgcagc aggcgctgca tcgcgatcgt gccgatcgcg ccggcggcgg ccccgaagtg
     4561 cgtgttcagc acgatgcggc cgatgccggc ctcggccacg gcggtcgagc cgccgttgaa
     4621 gccgaaccag ccg
//
LOCUS       DAOQJH010000116         4629 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_11043_length_4629_cov_3.627022, whole genome shotgun sequence.
ACCESSION   DAOQJH010000116 DAOQJH010000000
VERSION     DAOQJH010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 4629)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4629)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4629
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11043_length_4629_cov_3.627022"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            266..472
                     /locus_tag="PLQ18_03905"
     CDS             266..472
                     /locus_tag="PLQ18_03905"
                     /inference="COORDINATES: protein motif:HMM:NF016028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SlyX family protein"
                     /protein_id="HNE00829.1"
                     /translation="MDEQRLIDLEIRYAHQDAALNELSDLVYRQGRELDALRRLVGDL
                     RRQLQAMAPSNIATAAEETPPPHY"
     gene            complement(501..989)
                     /locus_tag="PLQ18_03910"
     CDS             complement(501..989)
                     /locus_tag="PLQ18_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018748242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HNE00830.1"
                     /translation="MSKAKTPVTAAIRLLRQHKVGFSDHLYEYEERGGTAHSAHALGL
                     PEHAVVKTLIMEDDRHDPLIVLMHGDLQVSTKELARQIGARSIAPCAPEVANRHSGYL
                     VGGTSPFGTRHAMPVYVEATILELPRIWINGGKRGYLIGIEPSVLSTVLKPLPVRVGI
                     PG"
     gene            1153..2178
                     /locus_tag="PLQ18_03915"
     CDS             1153..2178
                     /locus_tag="PLQ18_03915"
                     /inference="COORDINATES: protein motif:HMM:NF017736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HNE00831.1"
                     /translation="MTTSKSVSLPLIEIDQPLLDAAEPCPQRRGFLRTAGLLVSSLIA
                     LPVLARSAGNGKHTVSTARSTAKSASASTARTSARAAKPAAGASVAGRAATGKRMPAK
                     PVIALRQAPAGKSNLKIRVDARGKPVGGRVVAQIRGGSTYKPQLRSTGRGYVDRGEQR
                     IGRGSIVADTRGLEAPGIITESKTASTESLFRNGDRVLSLYAPGTGESVREVFWTPNT
                     GYLPQSIKEISWALRDHHNDEFRLFDPRALDILYAMQLMLQRPETHIISGYRSPETNA
                     LLREESRGVAKNSFHMQARALDVRMPGLGVGQMRMAALSLRAGGVGYYPRSNFVHIDS
                     GPVRSWG"
     gene            2362..3984
                     /locus_tag="PLQ18_03920"
     CDS             2362..3984
                     /locus_tag="PLQ18_03920"
                     /inference="COORDINATES: protein motif:HMM:NF018332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNE00832.1"
                     /translation="MSAVPLAGTEPVPAAGTAAPAGLYGAQALRAMLGIALVAMLVAL
                     DQTVVGTAMPTIVAELRGFEQFAWVATAYLLSSTTLLPVLGRLGDLFGRKPFVIAAII
                     VFTGASALCGQADSMPALIAARALQGVGGAMIIATAFTSIADLFPDTAHRLRWQALMS
                     SSFALASGIGPVLGGLLTEHFGWRSVFYVNLPLGLLALVLVVAYLPRSRGTRAEGAGF
                     DLPGALWLSGGLAALLLWVELGQRAGAWFAGALPLWLAGGVLALLLFVRRQLHARHPL
                     LPLRLLAIPRVRRVVLCALAAGAVMFAALFYAPLLLQGAYGHSPHEAGLIVTPLVLAI
                     ALGSLINGRLYGRLADPESLLLPGWLLLGAGVLGVQAVGAQTSAWLAAGAFLLCGLGL
                     GFLFPNFTLLVQTSVPRAELGVATALVQTVRALGSMAGTVVLGVLVTGTWRTAMDAAG
                     SGAGAQALRPLFADPQLLLDPAGLHQATTLAARLPDGPAQLAQLLDAAHRALAAGIHL
                     GLLLAAGLAALVLIALLAGRWRRRGRVAEAGT"
     gene            3981..4415
                     /locus_tag="PLQ18_03925"
     CDS             3981..4415
                     /locus_tag="PLQ18_03925"
                     /inference="COORDINATES: protein motif:HMM:NF024212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNE00833.1"
                     /translation="MSQSASALHHMVRLAQRLSTAFEAAVGMHLARWRILNHLHEHGA
                     STQKHMKTLIGVDAAALTRQLQLLEAEGLVRRRSHPDDQRLTEVALTAAGTDRLAAGR
                     VARDAFVARLLAGLDAEALAAFEATLAHIGRNLDARTQEPDA"
BASE COUNT          640 a         1626 c         1639 g          724 t
ORIGIN      
        1 gtgcgcgacc acaggcagac gcggtaagca gtttcgagcg ggccgccgaa gctgacgccg
       61 ccgcggccct cgcgcaccgg cgcggcgccg agcgcggcga gttcggcggc gagcagcggt
      121 tcgacgccgc gcggggcggt cgcgaaaaac ggaagatcgg cagacatcgg cgcgggtacc
      181 gggcaggcgc gggccggcat tctagcgtcc ggccgcgcgg cggctagaat tcgcgccccc
      241 cgacccgtac ccggagcccg ccgccgtgga cgaacagcgc ctgatcgatc tggagattcg
      301 ctacgcgcat caggacgccg cgctcaatga actcagcgac cttgtctacc gccaggggcg
      361 cgagctcgac gcgctgcgcc ggctggtcgg cgatctgcgc cggcagctgc aggcgatggc
      421 accgtcgaac atcgcgaccg cggccgagga aacgccgccg ccgcactact gatgcggact
      481 gatgcggact gatgcgcacc tcagccgggg atgccgaccc gcaccggcag cggtttcagc
      541 accgtgctca agaccgacgg ctcgatgccg atcaggtagc cacgcttgcc gccgttgatc
      601 cagatgcgcg gcagttcgag gatcgtcgcc tcgacgtaga ccggcatcgc gtggcgcgtg
      661 ccgaacggcg aggtgccgcc gaccagatag ccgctgtggc ggttggcgac ctcgggtgcg
      721 cagggggcga tcgagcgggc gccgatctga cgcgcgagtt ccttcgtcga cacctgcagg
      781 tcgccgtgca tcagcacgat cagggggtca tgccggtcgt cttccatgat cagcgtcttc
      841 acgacggcgt gctcgggcaa ccccagcgca tgggcgctgt gggcggtgcc gccgcgctct
      901 tcatattcgt ataaatgatc cgaaaacccg actttgtgct gtcgcaacaa gcggatggcg
      961 gcagtgacgg gggttttggc cttactcatg aacatgatct caatcaagta tttgatggat
     1021 tacgattttg taaaaaagca gcggtattgt tgcaaacctg atacgagtgt cgttttgaga
     1081 atttaagttt gcgtctgtgt gtaaatttgt tttaataatc tcataaagtt tgtcgattcg
     1141 aggttccgac ggatgaccac ttccaagagc gtgtcgctgc cgctgatcga aatcgatcaa
     1201 ccgctgctgg atgcggccga gccctgcccg cagcggcgcg gcttcctgcg caccgccggc
     1261 ctgctggtca gcagcctgat cgcgctgccg gtgctggccc gcagcgccgg caacggcaag
     1321 catacggtca gcacggcccg cagcaccgca aagtccgcat ctgcgtccac ggcccgcacg
     1381 tccgcccgcg ccgcaaagcc ggctgccggg gccagcgtgg ccggtcgtgc cgcgaccggc
     1441 aagcgcatgc cggcgaagcc ggtgatcgcg ctgcgtcagg cgccggccgg caagagcaac
     1501 ctgaagatcc gcgtcgatgc ccggggcaag ccggtcggcg ggcgcgtggt cgcgcagatc
     1561 cgcggcggca gtacctacaa gccgcagctg cgcagcaccg gccgcggcta tgtcgatcgc
     1621 ggcgagcagc gcatcggccg tggcagcatc gtggccgaca cccgcggcct cgaagcgccg
     1681 ggcatcatca ccgagtcgaa aaccgcgtcg accgagagcc tgttccgcaa cggcgaccgc
     1741 gtgctgtcgc tgtacgcgcc gggcaccggc gagagcgtgc gcgaggtgtt ctggacgccg
     1801 aacaccggct acctgccgca gtcgatcaag gaaatctcct gggcgctgcg cgatcaccac
     1861 aacgacgagt tccggctgtt cgatccgcgg gcgctcgaca tcctgtacgc gatgcagctg
     1921 atgctgcagc ggcccgagac ccacatcatc tccggctacc gctcgcccga gaccaacgcg
     1981 ctgctgcgcg aggaatcgcg cggcgtcgcc aagaacagct tccacatgca ggcgcgcgcg
     2041 ctcgacgtgc gcatgcccgg actcggcgtc ggccagatgc gcatggcggc gctgagcctg
     2101 cgcgccggtg gcgtcggcta ttacccgcgc agcaacttcg tgcacatcga ctccggcccg
     2161 gtgcgcagct ggggctgatc cccgcgccgc cttgccgtgc gctgccgacg ccgccccccg
     2221 gggcggcgtc gtcgtttccg gggcaggaca agcacacacc ggtgcatggc agccatgagc
     2281 ctgatttgat cgatcaatga ttgatcggtc aatcatccgg cgcgatgctg gcggcctttc
     2341 ccccgccgga gtccgtcgcc gatgtccgcc gtccccctcg ccggaaccga gcccgtgccg
     2401 gccgccggca ccgccgcgcc tgccggcctg tacggcgcgc aggccctgcg cgcgatgctc
     2461 ggcatcgcgc tggtcgcgat gctggttgcg ctcgatcaga ccgtggtcgg cacggcgatg
     2521 ccgaccatcg tcgccgagct gcgcggcttc gagcagttcg cgtgggtcgc gaccgcctat
     2581 ctgctgagct cgacgacgct gctgccggtg ctcggccggc tcggcgacct gttcgggcgc
     2641 aagcccttcg tgatcgcggc gatcatcgtc ttcaccggcg cctcggcgct gtgcggacag
     2701 gccgactcga tgccggcgct gatcgcggcg cgtgcgctgc agggcgtcgg cggcgcgatg
     2761 atcatcgcca ccgccttcac gagcatcgcc gatctgttcc cggacaccgc gcaccggctg
     2821 cgctggcagg cgctgatgtc gagcagcttc gcgctcgcga gcggcatcgg gccggtgctc
     2881 ggcgggctgc tgaccgagca cttcggctgg cgctcggtgt tctacgtcaa cctgccgctc
     2941 gggctgctgg cgctggtgct ggtcgtcgcc tatctgccgc gcagccgcgg cacgcgcgcc
     3001 gagggcgccg gcttcgacct gcccggtgcg ctgtggctga gcggcgggct cgcggcgctg
     3061 ctgctgtggg tcgaactcgg ccagcgcgcc ggcgcgtggt tcgccggtgc gctgccgctg
     3121 tggctggccg gcggcgtgct ggcgctgctg ctgttcgtgc gccggcagct gcacgcccgc
     3181 catccgctgc tgccgctgcg gctgctcgcg atcccgcgcg tgcgccgcgt cgtactctgc
     3241 gcgctcgcgg ccggggccgt gatgttcgcg gcgctgttct acgcaccgct gctgctgcag
     3301 ggcgcttacg gccattcgcc gcacgaggcc gggctgatcg tgacgccgct ggtgctggcg
     3361 atcgcgctcg gcagcctgat caacggccgg ctgtacgggc ggctcgccga tccggaatcc
     3421 ctgctgctgc ccggctggct gctgctcggg gccggcgtgc tcggcgtgca ggcggtcggc
     3481 gcgcagacct cggcgtggct tgcggccggc gccttcctgc tatgcgggct cggactcggc
     3541 ttcctgttcc cgaacttcac gctgctggtg cagaccagcg tgccgcgcgc tgaactcggc
     3601 gtggcgacgg cgctggtgca gaccgtgcgc gcgctcggca gcatggccgg cacggtcgtg
     3661 ctcggcgtgc tggtcaccgg cacctggcgt acggcgatgg acgccgccgg cagcggtgcc
     3721 ggcgcgcagg cgctgcggcc gctgttcgcc gatccgcagc tgctgctcga tccggccggt
     3781 ctgcatcagg ccacgacgct ggcggcgcgc ctgccggacg gcccggcgca gctcgcgcag
     3841 ctgctcgatg ccgcgcaccg ggcgctcgcg gccggcatcc accttggcct gctgctcgcg
     3901 gccgggctcg ctgcgctggt gctgatcgcg ctgctcgccg gccgctggcg gcggcgcggc
     3961 cgggtggcgg aggcggggac atgagccagt ctgcttccgc gctgcatcac atggtgcggc
     4021 tcgcgcagcg gctgtcgacg gccttcgagg cggcggtcgg catgcatctg gcgcgctggc
     4081 gcatcctgaa ccacctgcac gagcacgggg ccagcacgca gaagcacatg aagacgctga
     4141 tcggcgtcga tgccgcggca ttgacccggc agctgcagct gctcgaagcc gaaggtctgg
     4201 tgcgccggcg cagccatccg gacgaccagc gcctgaccga ggtggcgctg accgcggccg
     4261 ggaccgatcg cctcgccgcc gggcgcgtgg cccgcgatgc cttcgtcgcg cgcctgctgg
     4321 ccggcctcga tgccgaagcg ctcgcggcct tcgaggcgac gcttgcgcac atcgggcgca
     4381 acctcgatgc ccggacgcag gaacccgacg cctgacccgc cgcagccggg ccggcggcgg
     4441 atggtgcagc gtgaaaaacc gaaggcccgg cttcgcctgc cgggccttcg cagaggggcc
     4501 gcgtcgatca gccgtgcatc acatgacgcc gagcagcttc ggcagcgtca gcgagatcgc
     4561 cggcacgtag gtcacgatca cgaggaagac cagcatcgtc agcagccacg gccacacggc
     4621 gatggtcag
//
LOCUS       DAOQJH010000117         4628 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_11046_length_4628_cov_6.770391, whole genome shotgun sequence.
ACCESSION   DAOQJH010000117 DAOQJH010000000
VERSION     DAOQJH010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 4628)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4628)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4628
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11046_length_4628_cov_6.770391"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3538
                     /gene="hrpA"
                     /locus_tag="PLQ18_03930"
     CDS             <1..3538
                     /gene="hrpA"
                     /locus_tag="PLQ18_03930"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HNE00834.1"
                     /translation="TQPRRIAARSTAARIAQELKTELGSLVGYKIRFTERTIPETFVK
                     LMTDGILLAETQGDPSLSQYDTLIIDEAHERSLNIDFLLGYLKTLLPKRPDLKVIITS
                     ATIDAERFSRHFHDAPVIEVSGRLFPVEVRYRPVETEEAEREDQRDLYDALVDACDEL
                     HREGPGDVLVFLPGEREIRDAAEALRKHHPPHTEILPLFARLSAEEQSRIFKPHGGRR
                     IVLATNVAETSLTVPGIRYVVDTGLARVKRYSYRNKVEQLQVEKISQAAANQRAGRCG
                     RVAAGICIRLYDEMGFKARSAFTDPEILRSSLAGVILRMKSLHLGDIEEFPFLEAPSS
                     RAVADGYQLLQELNALDGNNVLTGIGHGLAKLPLDPRIARMIVAARDGGCLKEVLVIA
                     AALSVQDPRERPQEKQGTADAAHAKWADEKSEFLSWLKLWNWYENEIDHKKSQRKLVQ
                     VCHASFLSANRMREWRDIHSQLHALIGEQGWKENEIPATYDAIHKALLTGLLGNLGCR
                     SEDSMYFLGAHGIKFLVHPSSPLQKKVGRWLMASEITETSKLYARCIARVDPLWLEEV
                     GAHLIRRSYFDPHWEQKAMQVAAWERSTLYGIVVNPKKRVNFGPMNPAEAREIFIRQG
                     LVGGEINEDFARRWPFWQHNKKLMAEIESLEHKSRRPDVLVDDELIYAFYDHVIPEGI
                     HNGADFDKWRKEAERENAKLLFLKREDLMRHEAAGVTTDAFPSQIKLGGVEYALTYHF
                     EPGSPKDGVTLTVPLPHLNQVPALRCEWLVPGLLKEKVLQLVKTLPQKIRAKLVPVPE
                     FAAEFAEKVVPPDRGLVPALIAFIMETRGLNARGWAVTPDAFRPGELPAHLSMNFKLV
                     DEHGRQLDMRRNLTELRAEWGGQAKTEFAEKHEAPSEFSGLTDWTFGELPELMEVEVG
                     GQMLLGYPGLTDDGESVSLKVFDSPEEARAAHEKGLVRLFKIQFKEQLKYFEKNLPGL
                     PQMGIQAMGLMTVDELKQQLIDIVFARACLTAPLPQTEAQFRMRCTEAKSRLGLLSQE
                     ICRLVSLVLTEWQALMKKLPGFKAHAAAVKDIEVQVARLMGKRFVVATPFERLQHYPR
                     YMKAAALRLDKLKADPARDSRLMAEYAPLWTNYERRAGQLAKQGVVDENLEQFRWLLE
                     ELRVSLFAQELKTPTPVSTKRLQKTWEGLARG"
     gene            3531..4271
                     /locus_tag="PLQ18_03935"
     CDS             3531..4271
                     /locus_tag="PLQ18_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EI24 domain-containing protein"
                     /protein_id="HNE00835.1"
                     /translation="MVEIFNALMRSLNNLQDRRIWLRLAGPALASLLVWLLLAFFALE
                     WFVAQLLDFPPLTWIAGWGALWLAKVLGWVGGWLAVFALAYMTAMLLAAIFIVPLLVN
                     EIGASEYPDVARAGEDSLVAATANSLVAAAGFLIGWIGTLPLWLIPGMGLVLPIYWLA
                     WLNRRTFAYDALAAHATKDEWKALFDKHRGKLLLLGVLLGLLAHVPIIGLLAPAFGAL
                     AYVHYGLEALRRERDAAAVIIEGEARRV"
     gene            4268..>4628
                     /locus_tag="PLQ18_03940"
     CDS             4268..>4628
                     /locus_tag="PLQ18_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004369733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HNE00836.1"
                     /translation="MNFGAIIIGDEILSGKRQDKHFAKIAEMLGARGLRLSWVEYLGD
                     DRERLTETFRRTLASGDVVFSCGGIGNTPDDHTRQAVAAALGVGLEISPEGVEEARIR
                     FGDDMTPERLQLVTFPAG"
BASE COUNT          800 a         1497 c         1556 g          775 t
ORIGIN      
        1 tacccagccg cgccgcatcg ccgcgcgctc gaccgccgcg cgcatcgcgc aggagctgaa
       61 gaccgaactc ggcagcctgg tcggctacaa gatccgtttc accgaacgga cgatcccgga
      121 aactttcgtc aagctgatga ccgacggcat cctgctcgcc gagacgcagg gcgatccttc
      181 gctgtcgcag tacgacacgc tgatcatcga cgaggcgcat gagcgcagcc tgaacatcga
      241 tttcctgctc ggctacctga agacgctgct gccgaagcgg ccggacctga aggtaatcat
      301 cacttccgcg acgatcgatg ccgagcgctt ctccaggcat ttccacgatg cgccggtgat
      361 cgaggtctcc ggccgcctgt tcccggtcga ggtgcgctac cgcccggtgg aaaccgaaga
      421 agccgaacgc gaggatcagc gcgacctcta cgacgcactg gtcgacgcct gcgacgagct
      481 gcaccgcgag gggccgggcg acgtgctggt cttcctgccc ggcgagcgcg aaatccgcga
      541 cgcagcagaa gcgctgagaa agcatcaccc gccgcacaca gagatattgc cgctgttcgc
      601 ccgcctctcc gccgaggagc agtcgcgcat cttcaagccg cacggcggcc gccgcatcgt
      661 gctggcgacc aacgtcgccg aaacctcgct gacggtgccg ggcatccgct acgtcgtcga
      721 taccgggctg gcgcgcgtca agcgctactc gtatcgcaac aaggtcgagc agctgcaggt
      781 cgaaaagatc tcgcaggccg ccgccaacca gcgtgccggc cgctgcggcc gcgtcgccgc
      841 cggcatctgc atccggcttt acgacgagat ggggttcaag gcgcggtcgg ccttcaccga
      901 tccggaaatc ctgcgctcct cgctggcggg ggtgatcctg cgcatgaagt cgctgcatct
      961 cggcgacatc gaggagtttc ccttcctcga ggcgccgtcg tcgcgtgccg tcgccgacgg
     1021 ctaccagctg ctgcaggaac tgaacgcgct cgacggcaac aacgtcctga ccggcatcgg
     1081 ccacgggctg gccaagctgc cgctcgaccc gcgcatcgcc cgcatgatcg tggccgcccg
     1141 cgacggcggc tgcctgaagg aagtcctggt catcgccgcc gccctctccg tgcaggaccc
     1201 gcgcgaacgg ccgcaggaga agcagggcac ggccgatgcc gcgcacgcca agtgggccga
     1261 cgagaagtcg gaattcctct cctggctcaa gctgtggaac tggtacgaga acgagatcga
     1321 tcacaagaag tcgcaaagga agctggtgca ggtctgccat gccagcttcc tctcggccaa
     1381 tcgcatgcgc gagtggcgcg acatccattc gcagctgcac gccttgatcg gcgagcaggg
     1441 ttggaaggaa aacgagattc ccgcgaccta cgacgcgatc cacaaggcgc tgctcaccgg
     1501 ccttctgggc aacctcggct gccgttccga ggattcgatg tacttcctcg gcgcgcacgg
     1561 catcaagttc ctcgtccatc cctcgtcgcc gctgcagaag aaggtcggcc gctggctgat
     1621 ggcgagcgag atcaccgaga cgagcaagct ctacgcacgc tgcatcgccc gcgtcgatcc
     1681 gctgtggctg gaggaagtcg gcgctcacct gatccgccgc agctacttcg atccgcactg
     1741 ggagcagaag gcgatgcagg ttgccgcctg ggagcgctcg acgctctacg gcatcgtggt
     1801 gaacccgaag aagcgggtga atttcggccc gatgaacccg gccgaggcgc gcgagatctt
     1861 catccgccag ggtctggtcg gcggcgagat caacgaggac ttcgcgcggc gctggccgtt
     1921 ctggcagcac aacaagaagc tgatggccga gatcgagtcg ctcgagcaca agtcgcgtcg
     1981 cccggacgtg ctggtcgacg atgagctcat ctacgccttc tacgaccatg tgatcccgga
     2041 gggcatccac aacggcgccg acttcgacaa gtggcgcaag gaagccgagc gcgaaaacgc
     2101 caagctcttg ttcctcaagc gcgaggacct gatgcgccat gaggcggccg gcgtcacgac
     2161 cgacgccttc ccctcgcaga tcaagctcgg cggtgtcgag tacgcactca cctatcactt
     2221 cgagccgggc tcgccgaagg acggcgtgac gctgaccgtg ccgctgccgc acctcaacca
     2281 ggtgccggcg ctgcgctgcg agtggctggt gccgggcctg ctcaaggaaa aggtgctgca
     2341 gctggtgaag acgctgccgc agaaaatccg cgccaagctg gtgccggtgc ccgagttcgc
     2401 ggccgagttt gccgagaagg tggttcctcc cgaccggggg ctggtgccgg cactgatcgc
     2461 cttcatcatg gaaacgcgcg ggctgaatgc acggggatgg gcggtgacgc cggacgcctt
     2521 ccgccccggc gagttgccgg cgcacctgtc gatgaacttc aagctggtcg acgagcacgg
     2581 ccgccagctc gacatgcgcc gcaacctgac cgagctgcgt gccgagtggg gcgggcaggc
     2641 gaagaccgag ttcgccgaga agcatgaggc gccctccgaa ttctccggcc tcaccgactg
     2701 gaccttcggc gaactgccgg aactgatgga ggtcgaggtc ggcggccaga tgctgctcgg
     2761 ctacccgggg ctgaccgatg atggtgaatc cgtttcgctg aaggtcttcg attcacccga
     2821 ggaggcgcgc gccgcgcacg agaaggggct ggtgcggctg ttcaagatcc agttcaagga
     2881 gcagctcaag tacttcgaga agaacctgcc cggcctgccg cagatgggca ttcaggcgat
     2941 gggtctgatg acggtcgacg aactgaagca gcaactgatc gacatcgtct tcgcccgcgc
     3001 gtgcctgacg gcgccgctgc cgcaaacgga agcgcagttc cgtatgcgct gcaccgaggc
     3061 gaagtcgcgg ctcggtctgc tctcgcagga aatctgtcgc ctcgtcagtc tggtgctcac
     3121 cgaatggcag gcgctgatga agaagctgcc ggggttcaag gcccatgcag cagcggtgaa
     3181 ggacatcgag gtgcaggtgg cgcggctgat gggcaagcgc ttcgtcgtcg cgaccccgtt
     3241 cgagcggctg cagcactacc ctcgttatat gaaggcggcg gccttgcgcc tcgacaagct
     3301 gaaggccgat ccggcccggg actctcgcct gatggccgaa tacgcgccgc tgtggacgaa
     3361 ctacgagcgc cgtgccgggc aactcgccaa gcagggcgtg gtcgacgaga atctggagca
     3421 gttccgctgg cttcttgagg agctgcgggt gtcgctgttc gcgcaggagc tgaagacgcc
     3481 gacaccggtg tcgaccaagc gcctgcagaa aacctgggag ggcctggccc gtggttgaaa
     3541 tcttcaatgc gttgatgcgc agtctgaaca acctgcagga ccgccgcatc tggttgcggc
     3601 tggcggggcc ggcgctggcc tcgctgctgg tgtggctgct gctcgctttc ttcgcgctcg
     3661 aatggttcgt ggcgcagctg ctggactttc cgccactgac ctggatcgcc ggctgggggg
     3721 cgctgtggct ggccaaggtg ctgggctggg tcggcggctg gctggccgtg tttgcgctgg
     3781 cctacatgac ggcgatgctg ctggcggcga tcttcatcgt gcccttgctg gtcaacgaga
     3841 tcggcgcgag cgagtatccg gatgtcgcac gggccggcga ggacagcctc gtcgccgcca
     3901 cggccaacag cctcgtcgct gccgccggct tcctcatcgg ctggattggc accctgccgc
     3961 tgtggctgat tcccggcatg gggctggtcc tgccgatcta ctggctggcc tggctgaacc
     4021 ggcgcacctt tgcctacgac gcgctggcgg cgcatgcgac gaaggatgaa tggaaggcgc
     4081 tgttcgacaa gcatcgcggg aagctgctgc tgctcggtgt cctgctcggt ctgctcgcgc
     4141 atgtgccgat catcggcttg ctggccccgg cgttcggtgc gctggcgtat gtgcattacg
     4201 gactggaggc gctgcgtcgc gaacgtgacg cggcggcggt catcatcgaa ggggaggcaa
     4261 gaagggtatg aactttggcg cgatcatcat cggtgacgag attctttccg gcaagcggca
     4321 ggacaagcac ttcgcgaaga tcgcggagat gctcggtgcc cgcggcttgc gcctctcctg
     4381 ggtcgagtat ctcggcgacg accgcgagcg cctgaccgaa accttccggc gtacgctggc
     4441 ctcgggcgat gtggtgttct cctgtggcgg catcggcaac acgcccgatg accacacgcg
     4501 ccaggccgtg gcggcggcgc tcggcgtcgg cctcgagatc agcccggagg gcgtcgagga
     4561 agcgcggatc cgtttcggcg acgacatgac gccggagcgc ctgcaactgg tgaccttccc
     4621 ggccggca
//
LOCUS       DAOQJH010000118         4609 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_11120_length_4609_cov_3.296882, whole genome shotgun sequence.
ACCESSION   DAOQJH010000118 DAOQJH010000000
VERSION     DAOQJH010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 4609)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4609)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4609
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11120_length_4609_cov_3.296882"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1816
                     /locus_tag="PLQ18_03945"
     CDS             <1..1816
                     /locus_tag="PLQ18_03945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE00837.1"
                     /translation="WADGNGVYVLDGGTLDVATDAVVGNRGNGRFEQTGGTFTVANAL
                     IIGNRDATHGEYEQSGGTTQVDGELIVGRPAGSTGSYTLSAGRLNVSADAWIGRAGSG
                     TFTQTGGLAIIGGDLLIGAEAGGTGRYRLDSGTLAVGGSIVLGGTTTADGGSGELTLS
                     GSANLYAHGLDLRPGGTLGGLGGGITLGDSEQGNAAAGTIMVNRGGTLAGNGTIAGSI
                     TVNDGGTLSPGNSIGTLSVGDVAFASGSTLRIEVDGAGNADLLHSTGNITISGATLRI
                     DSADGTYSADANYTLLTADGRINGTFTNVVTSLPLLAQTATYTGNVVTLVVPTPDENA
                     SRSPLDLIGGSPVSLLGRMTLADVEAGFLLDPLVTGIDEGQAGIGARIGRYHADGYRQ
                     TYEEMPLRYAWKLDSGLTVLAELPIANISTSGRGVDRRDRSYGFGGGLRVPVAAGWDL
                     KPMFRYGTVVDEDWDHVGSLASASLTSHFRTSFGNGYEFGADNLIGYFQSVGGRVEGL
                     NAGYRLHEVALHNAVRVGGPLEGTFLGSGARWTAWISDTRFLGSELAADNFQRIGIAA
                     STRVRVGERFSENLNLGLHYTQSAGGDHGVELNFGYRF"
     gene            1836..2489
                     /locus_tag="PLQ18_03950"
     CDS             1836..2489
                     /locus_tag="PLQ18_03950"
                     /inference="COORDINATES: protein motif:HMM:NF024790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HNE00838.1"
                     /translation="MSALRFWLLLLVAAALSALPLIGLPLRWAGTFFHELSHGLMAAA
                     TGGDIVRIVLRTDGSGLCVFTGGWGVPVAFAGYAGAPLWGLGIFALASGDSQRDDAPI
                     LAGLLGGTVAITALLYARDAVTIVISALLVALLLLPLRRRWRNGRALQAGLEVLGLAV
                     MLDAVQAPLALFGLSGRHDGADLAAHTWLPAPLWAGLWCVLAGACLWLAWRRVRALR"
     gene            complement(2571..2885)
                     /locus_tag="PLQ18_03955"
     CDS             complement(2571..2885)
                     /locus_tag="PLQ18_03955"
                     /inference="COORDINATES: protein motif:HMM:NF020918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HNE00839.1"
                     /translation="MTQFSVPSLESLSAPVVKAGKLNVASLEKLTAFNFSLLQSYSDL
                     SLARLKAATDVTDLASLKAYGESSVEVAKELGAKLQADAKAYADLLAGVGAEYKDLVK
                     AA"
     gene            complement(3017..>4609)
                     /locus_tag="PLQ18_03960"
     CDS             complement(3017..>4609)
                     /locus_tag="PLQ18_03960"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNE00840.1"
                     /translation="VELRPESADAQMRHGLAQLALGEHDAALQSLGAAAGRAPGAQEQ
                     ARALLLARLASGRHAEALAQAQALSAAHPEAVEPLLYAGAAHEGLQDLPAARADYERA
                     LALVPGQAQAAAALARLDLLDGRPDSARARYQAVLARSPRHVPTLMALARLEWLHGRP
                     EAAIAALETVAGIAPEAVAPQLLLARLQAQHGRLADAVTRLDSLHFAHPDDEAVLAHL
                     AAARLQAGDIAETERLHRDWLARRPDSVAALYGLAVGAAARGDAEALRQALIPALQAD
                     REAAPAPALLTRLDALAADPARARAWLAQLQQAVPDHPALLDHAGERLGREQGAAKAA
                     AYYALLRGRQPDEARWPQREAVWREAAGEGAAAARMLETWLARHPQAVGLRLQLATLY
                     ERLDRLEDARAAFVLVLSQRPDEVVALNDLAWLLRDRDPQQALVHARRAALLAPRPEV
                     LETLGEVLIAAGQPEAAIGVLDTASRGEPSASSIRLNLARAQLASGRVEAARTTLRAL
                     LAGAQSFEGRDAALALLGRAGG"
BASE COUNT          602 a         1654 c         1692 g          661 t
ORIGIN      
        1 ctgggccgac ggcaacggcg tctacgtgct cgacggcggc acgctcgacg tggcgaccga
       61 cgccgtggtc ggcaaccgcg gcaacggccg cttcgagcag accggcggca cgttcaccgt
      121 cgccaatgcc ctgatcatcg gcaatcggga cgccacgcac ggcgagtacg aacagtccgg
      181 cggcacgacg caggtcgacg gcgagctgat cgtcggccgc cccgcgggct cgaccggcag
      241 ctacaccctg tccgccggca ggctcaacgt cagcgcggat gcctggatcg gccgggccgg
      301 cagcggcacc ttcacgcaga ccggcgggct cgccatcatc ggcggcgacc tgctgatcgg
      361 tgccgaagcc ggcggcaccg gccggtaccg gctcgacagc ggcacgctgg ccgtcggcgg
      421 cagcatcgtg ctcggcggca cgaccacggc cgatggcggc agcggcgaac tgacgctctc
      481 gggcagcgca aacctgtacg cgcacggcct cgacctgcgt ccgggcggca cgctgggcgg
      541 gctgggcggc ggcatcacgc tcggcgactc tgaacagggc aacgccgccg ccggcacgat
      601 catggtcaat cgcggcggca cgctcgccgg taacggcacg attgcgggca gtatcaccgt
      661 caacgatggc ggcaccctca gccccggcaa ctcgatcggc acgctgagcg tcggcgacgt
      721 tgccttcgcc agcggctcga cgctgcgcat cgaggtcgac ggggccggca acgccgatct
      781 gctgcactcg accggcaaca tcacgatcag cggtgccacg ctgcgcatcg actcggccga
      841 cggcacgtac tcggccgacg cgaactacac gctgctgacc gccgacggcc gtatcaacgg
      901 caccttcacg aatgtcgtga cctcgctgcc gctgctcgca cagaccgcca cctataccgg
      961 caacgtcgtg acgctggtcg tgccgacccc ggacgagaac gcctcgcgct cgccgctcga
     1021 cctgatcggc ggcagccccg tgagcctgct cggacgcatg acgctcgccg atgtcgaggc
     1081 cggcttcctg ctcgacccgc tggtcaccgg catcgacgag ggtcaggccg gcatcggcgc
     1141 gcgcatcggg cgctaccacg ccgacggcta ccgccagacc tacgaggaaa tgccgctgcg
     1201 ctatgcatgg aagctcgaca gcggcctgac cgtgctcgcc gagctgccga tcgcgaacat
     1261 ctcgaccagc ggccggggcg tcgatcgccg tgaccgcagc tacggcttcg gcggtggcct
     1321 gcgcgtaccg gtcgccgccg gctgggacct gaaaccgatg ttccgttacg gcaccgtcgt
     1381 cgacgaggac tgggaccacg tcggctcgct ggcctcggcc tcgctgacca gccacttccg
     1441 gacgtccttc ggcaatggtt acgagttcgg cgccgacaac ctgatcggct acttccagtc
     1501 ggtcggcggt cgtgtcgaag gactgaacgc cggctaccgc ctgcacgaag tcgcgctgca
     1561 caacgccgtg cgcgtcggcg ggccgctgga gggcaccttc ctcggcagcg gcgcgcgctg
     1621 gacggcgtgg atctcggaca cgcgcttcct cggttcggag ctcgcggccg acaacttcca
     1681 gcgcatcggc atcgccgcga gcacgcgcgt gcgcgtcggt gaacgcttct ccgagaacct
     1741 gaacctcggc ctgcattaca cgcagtcggc cggcggcgat cacggcgtcg agctgaactt
     1801 cggctaccgc ttctgagacc cgggaggctg tgccgatgag cgcactgcgg ttctggctgc
     1861 tgctgctcgt ggcggcagcc ctgtcggcgc tgccgctgat cgggctgccg ctgcgctggg
     1921 ccggcacctt cttccatgag ctcagccacg gactgatggc cgcggcgacc ggtggcgaca
     1981 tcgtgcgcat cgtgctgcgc accgacggct ccggactgtg cgtgttcacc ggcggctggg
     2041 gcgtgccggt ggctttcgca ggctacgcag gggcgccgct gtgggggctc ggcatcttcg
     2101 cgctggcgag cggggattcg cagcgcgacg acgcgccgat cctcgccggc ctgctcggtg
     2161 gcacggtcgc gatcacggcg ctgctgtatg cgcgcgatgc ggtgacgatc gtgatctcgg
     2221 cgctgctggt cgcgctgttg ctgctgccgc tgcgccggcg ctggcgcaac gggcgggcgc
     2281 tgcaggccgg gctcgaagtc ctggggctgg ccgtgatgct cgatgccgtg caggcgccgc
     2341 tcgcgctgtt cgggctgtcc ggccggcatg acggtgccga tctggccgcg cacacctggc
     2401 tgccggcgcc gctgtgggcc gggctctggt gcgtactggc gggggcctgc ctgtggctcg
     2461 cctggcggcg ggtgcgcgcg ctgcgctgaa cgatggccgg gccggcggac cggaaaacaa
     2521 aaaccgcccg acggtgcggg cggttttcag gaaggtcggg gtgccggcga tcaggcggcc
     2581 ttgaccagat ccttgtactc ggcgccgacg ccggcgagca ggtcggcata ggccttggcg
     2641 tcggcctgca gcttggcacc cagctccttc gcgacttcga ccgaggactc gccgtaggcc
     2701 ttcaggctgg ccagatcggt cacgtcggtg gcggccttca ggcgcgccag gctcaggtcg
     2761 ctgtagctct gcagcaggct gaagttgaag gcggtcagct tttcgaggct ggcgacgttc
     2821 agcttgccgg ccttgacgac cggggcggac agggactcga gcgacggaac ggagaactgg
     2881 gtcatggctg catcctcgtt tgggtggcgg ggcggtcggg ttccggtgaa cccatgggtg
     2941 ccattatatc catgcgatgc tgcagtgcaa catccttttg agccttctgt cccgaccctg
     3001 ttcctttccc cgtgattcag ccgccggccc tgccgagcag cgcgagcgcg gcatcgcgtc
     3061 cttcgaagga ctgcgcaccg gcgagcagcg cgcgcagcgt cgtgcgggcc gcctcgacgc
     3121 ggccgctggc gagctgcgcc cgggcgaggt tcagccggat cgagctggcc gacggctcgc
     3181 cgcggctcgc cgtgtccagt acgccgatcg ccgcttccgg ctggcccgcg gcgatcagca
     3241 cttcgccgag tgtctccagc acctccggcc gcggggccag cagcgcggcc ctgcgggcgt
     3301 ggacgagcgc ctgctgcggg tcgcgatccc gcagcagcca ggcgaggtcg ttgagggcca
     3361 cgacctcatc gggccgctgg ctcagcacga gcacgaaagc ggcgcgcgcg tcttccagcc
     3421 ggtcgagccg ttcgtacagg gtcgcgagct gcaggcgcag cccgacggcc tgcggatgcc
     3481 gggcgagcca ggtctccagc atgcgggccg cggccgcccc ctccccggcc gcctcgcgcc
     3541 agactgcctc gcgctgcggc cagcgggcct cgtcgggctg ccggccgcgc agcagcgcgt
     3601 aataggccgc cgccttcgca gctccctgct cgcggccgag ccgttcgccg gcgtgatcga
     3661 gcagggccgg atgatcgggc acggcctgct gcagctgcgc gagccaggcc cgggcccggg
     3721 ccggatcggc cgcgagcgcg tcgagccgtg tgagcagggc tggcgccggc gcggcctcgc
     3781 gatcggcctg cagggccgga atcagggcct ggcgcagcgc ttcggcgtcg ccgcgggccg
     3841 ccgcgcccac ggccagtccg tacagcgccg ccaccgagtc cggccggcgc gcgagccagt
     3901 cgcgatgcag tcgttcagtc tcggcgatgt cgccggcctg caggcgcgcc gccgccagat
     3961 gcgccagcac ggcttcatcg tcgggatggg cgaagtgcag gctgtcgagg cgggtgacgg
     4021 cgtcggcgag ccggccgtgc tgcgcctgca ggcgggcgag cagcagctgc ggcgccacgg
     4081 cttcgggggc gattccggcc acggtctcca gcgccgcgat cgcggcttcg ggccggccgt
     4141 gcagccattc gagccgggcg agcgccatca gcgtcggcac atgccggggg ctgcgggcga
     4201 gcacggcctg atagcgtgca cgggctgaat cgggccggcc gtcgagcagg tcgagccgcg
     4261 ccagcgcggc agcggcctgc gcctggccgg gcacgagggc aagcgcgcgc tcgtaatcgg
     4321 cccgggccgc cggcaggtcc tgcagcccct catgcgccgc gccggcgtag agcagcggct
     4381 cgaccgcctc cggatgcgcg gccgacagcg cctgcgcctg cgccagggct tcggcgtgac
     4441 gaccgctggc gagccgggcg agcagcagcg cccgggcctg ctcctgcgca cccggcgcgc
     4501 gcccggcggc ggcaccgagt gactgcagcg ctgcgtcgtg ctcgccgagg gcgagctgcg
     4561 ccaggccgtg gcgcatctgc gcatcggccg attcgggcct gagttcgac
//
LOCUS       DAOQJH010000119         4592 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_11182_length_4592_cov_3.486665, whole genome shotgun sequence.
ACCESSION   DAOQJH010000119 DAOQJH010000000
VERSION     DAOQJH010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 4592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4592
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11182_length_4592_cov_3.486665"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..326
                     /locus_tag="PLQ18_03965"
     CDS             <1..326
                     /locus_tag="PLQ18_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007152465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Holliday junction DNA helicase RuvB C-terminal
                     domain-containing protein"
                     /protein_id="HNE00841.1"
                     /translation="RAGGHITQDIARAALAMLKVDEQGFDDMDRRLLGLLLDKFGGGP
                     VGLDTLAAALGEERGTIEDVLEPFLIQQGFLMRTPRGRTATAHAYRHFGLLPPGGADL
                     FDAPV"
     gene            335..1774
                     /locus_tag="PLQ18_03970"
     CDS             335..1774
                     /locus_tag="PLQ18_03970"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNE00842.1"
                     /translation="MAVTRHGGQRWPGSLRSLIIAGFLLALTPPLAALGWAAYTLNSL
                     VAEVDAGLETAVAAARYGRGLVADVTELQRSARHWNVLQSDTLLDTYRERRKRVDETL
                     GQLRALALDPERVQRLERLAAALPVGDGPPVGDDDVLTARLAPLVAQAVDVLELSDDA
                     VEGESARLREVTAEANRRFASLAALGLIAGSGCALLAGGLIARPTRQLAQAIRRLGDE
                     DFTTPVSLAGPRDIQLIGARLDWLRSRLGEIDAQKQRFLRHVSHELKTPLTALREGSE
                     LLRDGTLGPLAEDQREVAGILVDNARRLSTLIEDLLNFNRLLARTQQLEAGTVALAAL
                     VEEVAGAQRLAAQARCVTLELDVPEGLIVQADHDKLRTILDNLLSNALKFARRDGRIA
                     LRAGRYPGGVAVEVEDDGPGIPAEERERVFELFFQGSHQPDTPVRGSGLGLALAREFA
                     RLHGGDLIVADGRGSGACLCLTLPATAPP"
     gene            1890..2231
                     /locus_tag="PLQ18_03975"
     CDS             1890..2231
                     /locus_tag="PLQ18_03975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE00843.1"
                     /translation="MRRHSPPLLLCLLAVLGTGCDTLNGMTDKPPSGDEAILRKQLVD
                     ARSVADRARASARKAEARATDLGKDKADLETTVAELRAQNERLTQENLDLKRRLQALG
                     RIESTLSDQGK"
     gene            2233..3594
                     /locus_tag="PLQ18_03980"
     CDS             2233..3594
                     /locus_tag="PLQ18_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013331376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNE00844.1"
                     /translation="MTDKRCDILAVDDDPDLLRLLVLRLKAAGYVVETATNGQEALTQ
                     LTLCEPRLLITDLRMPGMDGLALFDAVRSQRPGLPVIMLTAHGTIPDAIQATRDGVFG
                     FLPKPFEPAELLAEVQRALATTAPARGNPAGWRAAIVTRSPRLESVLDEAQLVAKSDV
                     SVFVHGESGTGKELLAQAIHKASPRATRPFVPVNCGAIPSELLESELFGHVKGAFTGA
                     NTAHDGLFVAATGGTLFLDEIGDLPLAMQAKILRALQERQVRPVGSTRNVEVDVRIIS
                     ATHRDLDQLVADGSFREDLYYRLNVVELRLPPLRERREDVPLLVEQFLARLAEKAGEA
                     SKRFAPEAMEMLISSDWPGNVRQLANVVEHCVALSPSAVIPVALVRRALRDRNTPFPP
                     LEEARDAFEHDYLVRVLRLTRGGVTQAARLAGRNRTEFYRLLARHGLEPALFKAEAED
                     SED"
     gene            3821..4462
                     /locus_tag="PLQ18_03985"
                     /pseudo
     CDS             3821..4462
                     /locus_tag="PLQ18_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006451583.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
BASE COUNT          668 a         1725 c         1561 g          638 t
ORIGIN      
        1 tcagggccgg cggccacatc acgcaggaca tcgcccgcgc ggcgctggcg atgctgaagg
       61 tcgacgaaca gggcttcgac gacatggacc gccggctgct cggcctgctg ctcgacaagt
      121 tcggcggcgg cccggtcggg ctcgacacgc tggccgccgc gctcggcgag gagcgcggca
      181 cgatcgagga cgtgctcgaa cccttcctga tccagcaggg cttcctgatg cgcacgccgc
      241 gcgggcgcac cgcgaccgcg cacgcctacc ggcacttcgg cctgctgccc cctggcggcg
      301 ccgacctgtt cgatgcaccg gtctgagcac gcgcatggcc gtgacccgac atggcggcca
      361 gcgctggccg ggatccctgc gcagcctgat catcgccggc ttcctgctgg cgctgacgcc
      421 gccgctcgcc gcgctcggct gggcggcgta cacgctcaat tccctggtcg ccgaggtcga
      481 tgccgggctg gaaacggcag tcgccgcagc ccgctacggc cgcggtctgg tcgccgatgt
      541 caccgagctg cagcgttcgg cgcggcactg gaacgtgctg cagtccgaca cgctgctcga
      601 cacctaccgc gagcggcgca agcgcgtcga cgagacgctc ggccagctgc gggcgctcgc
      661 gctcgatccc gaacgcgtgc agcgcctcga acggctggcg gcggcgctgc cggtcggtga
      721 cggtccgccg gtcggcgatg acgacgtgct gaccgcgcgg ctcgcgccgc tggtcgcgca
      781 ggcggtcgac gtgctggagc tctccgacga cgcggtcgag ggcgagagcg cgcggctgcg
      841 cgaggtcacg gccgaggcca accggcgctt cgcgtcgctg gccgcgctcg ggctgatcgc
      901 cggcagcggc tgtgcgctgc tcgccggcgg cctgatcgca cggccgacgc gccagctcgc
      961 gcaggcgatc cggcgcctcg gcgacgagga tttcacgacg ccggtcagcc tcgccggacc
     1021 gcgcgacatc cagctgatcg gcgcgcggct cgactggctg cgcagccgcc tcggcgagat
     1081 cgacgcgcag aagcagcgct tcctgcgcca tgtctcgcac gagctgaaga cgccgctgac
     1141 cgcgctgcgc gagggctcgg agctgctgcg cgacggtacg ctcgggccgc tggccgagga
     1201 ccagcgcgag gtcgccggca tcctcgtcga caacgcccgg cggctgtcga cgctgatcga
     1261 ggacctgctg aacttcaacc gcctgctggc gcgcacgcag cagctcgaag cgggcacggt
     1321 ggcactcgcc gcgctggtcg aggaagtcgc cggcgcgcag cgcctggctg cgcaggcgcg
     1381 ctgcgtcacg ctcgagctcg acgtgccgga ggggctcatc gtgcaggccg atcacgacaa
     1441 gctgcgcacg atcctcgaca acctgctctc caacgccctg aagttcgccc ggcgcgacgg
     1501 ccgcatcgca ctgcgcgccg gccgctatcc cggcggcgtc gccgtcgagg tcgaggacga
     1561 cggtcccggc atcccggccg aggagcgcga gcgggtgttc gagctgttct tccagggcag
     1621 ccaccagccg gataccccgg tgcgtggctc cggtctcggc ctcgccctgg cccgtgaatt
     1681 cgcccgcctg catgggggcg acctgatcgt cgccgatggc cgcggcagtg gtgcctgcct
     1741 gtgcctgacg ctgccggcga ctgcaccacc gtagcgcgca aaaaaatccg gcgggcaccg
     1801 ggacaggact cttgaacccc gggggatcgc ctacaagtcg cgccacgcca aggctttttt
     1861 tcttccacca cccgctcggt gaccgcaaca tgcgccgcca ctctccgccc ctcctgctct
     1921 gtctgctggc cgtcctcggc accggctgcg acaccctcaa cggcatgacc gacaagccgc
     1981 cctcgggcga cgaggcgatc ctgcgcaagc aactggtcga tgcccgcagc gtcgccgacc
     2041 gcgcccgcgc cagcgcccgc aaggccgagg cgcgcgcgac cgatctcggc aaggacaagg
     2101 ccgatctcga aaccaccgtc gcggaactgc gcgcgcagaa cgagcgactg actcaggaga
     2161 atctcgatct gaagcgccgt ctgcaggcgc tcggccggat cgaatccacg ctttccgacc
     2221 agggcaaatg acgtgaccga caaacgctgc gacatcctcg cggtcgacga tgatcccgat
     2281 ctgctgcgtc tgctggtcct gcgcctgaag gccgccggct acgtcgtcga aaccgccacc
     2341 aacggtcagg aagccctgac ccagctcacg ctgtgcgagc cgcgcctgct gatcaccgac
     2401 ctgcgcatgc cgggcatgga cggcctggcg ctgttcgacg ccgtgcgcag ccagcgtccg
     2461 gggctgccgg tgatcatgct gactgcgcac ggcacgattc ccgatgccat ccaggccacg
     2521 cgcgacggcg tgttcggttt cctgccgaag cccttcgagc cggccgaact gctcgccgag
     2581 gtgcagcgcg cgctcgcgac cacggcgccg gcgcgcggca accccgccgg ctggcgcgcg
     2641 gcgatcgtca cgcgcagccc gcggctcgaa tccgtgctcg acgaggcgca gctcgtcgcc
     2701 aagtccgacg tctcggtgtt cgtgcacggc gagagcggca ccggcaagga actgctggcg
     2761 caggccatcc acaaggccag cccgcgcgcc acgcggccgt tcgtgccggt caactgcggc
     2821 gcgatcccgt ccgagctgct cgaatccgag cttttcggcc acgtgaaggg tgcgttcacc
     2881 ggcgccaaca ccgcgcacga cggcctgttc gtcgccgcaa ccggcggcac gctgttcctc
     2941 gacgagatcg gtgacctgcc gctggcgatg caggcgaaga tcctgcgcgc gctgcaggag
     3001 cgtcaggtgc gcccggtcgg ctcgacccgc aacgtcgagg tcgacgtgcg catcatctcg
     3061 gccacgcacc gcgacctcga ccagctcgtc gccgacggca gctttcgcga ggacctctac
     3121 taccgcctga acgtcgtcga gctgcgcctg ccgccgctgc gcgagcggcg cgaggatgtg
     3181 ccgctgctgg tcgagcagtt cctcgcgcgg ttggccgaga aggccggcga ggcgtcgaag
     3241 cgcttcgcgc ccgaggcgat ggagatgctg atttcctccg actggcccgg caacgtccgc
     3301 cagctcgcca acgtcgtcga gcactgcgtg gcgctgtcgc cgtcggcggt gatcccggtg
     3361 gcgctggtgc gccgcgcgct gcgcgaccgc aacacgccgt tcccgccact ggaggaggca
     3421 cgcgacgcct tcgagcacga ctatctggtg cgcgtgctgc gcctgacccg cggcggcgtc
     3481 acgcaggccg cgcggctggc cgggcgcaac cgcaccgagt tctaccggct gctcgcccgc
     3541 cacgggctgg agccggcgct gttcaaggcc gaggccgagg acagcgagga ctgaccgcca
     3601 tcccggcagc ggcctgccgg cgcagatcgc aacgacggcg cacacgtcgg gggacggggc
     3661 ccctccgcac gcagcaccgc tcgggatccg cagcgcacct gtccgcctgc atcaccgtct
     3721 gcacccttag cttccgccgg ccattccggc cgtgccgtgc gtgctgcatc cgcacaaccg
     3781 caatcccgcc cgtccggcgc ctcgggtatc cttgcccgcc atgcgcctgt cccggatacg
     3841 cctcgccgga ttcaagtcct tcgtcgatcc cgtcacggtc cacctgcgtt cgaacctcgt
     3901 cggcatcctc ggcccgaacg gctgcggcaa gtccaacgtc atcgacgccg tgcgctgggt
     3961 gatgggcgaa tcggcggcct cgcgcctgcg cggcgaggcg atgagcgacg tgatcttcag
     4021 cggttcggcg acgcgcaagc ccgtcggcca ggccagcgtc gaactggtgt tcgacaacgc
     4081 cgaaggccgc ctcggcggcg cctgggcgag ctacgccgag atctcggtgc gccgcctcgt
     4141 caaccgcgac ggcaccagcc agtacttcct gaacggcacg cgctgccggc ggcgcgacgt
     4201 gaccgagctg ttcctcggca ccggcctcgg gccgcgcagc tacgcgatcg tcgagcaggg
     4261 catgatctcg cgcgtcatcg acgcccggcc cgaggaactg cgtgccttcc tcgaagaggc
     4321 cgccggcatc tcgaagtaca aggaacgccg gcgcgagacc gaaacccgca tccgccacac
     4381 gcgcgagaac ctcgaccgcc tcgccgacgt gcgcgaggaa ctcgagcgcc gcctgcaggt
     4441 gctggctcgg caggcgcgca aggagagcgc ccggatccgg cgcaagcgca agaagcactc
     4501 cggctgaagt caagcgggcg gcccgacgct gcccgatcgc gcctccccgg ctcgatttga
     4561 caggccaccg catctgtact atactcccct ac
//
LOCUS       DAOQJH010000120         4584 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_11211_length_4584_cov_3.755575, whole genome shotgun sequence.
ACCESSION   DAOQJH010000120 DAOQJH010000000
VERSION     DAOQJH010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 4584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4584
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11211_length_4584_cov_3.755575"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..589)
                     /locus_tag="PLQ18_03990"
     CDS             complement(<1..589)
                     /locus_tag="PLQ18_03990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE00845.1"
                     /translation="MNMPTPTSEAAAAAALPGSLAPPGLPDVAGLTQLASAFFAMLPG
                     ETMAAALPGLAQRAPSLTAAQPPASLPGPVPLPTSLPVGHPAPPPAAVPPVTVPVAAT
                     GAGVPLVGSAPVAASAAPFYFLDEAGAGPRAAGGRDGLDDLDGLAAAASAPAVLPAQS
                     FGLPGESELQALLRSLTAPGPTHADGPYYFVDAPAP"
     gene            complement(564..1493)
                     /locus_tag="PLQ18_03995"
     CDS             complement(564..1493)
                     /locus_tag="PLQ18_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004631502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0140737 - encapsulin nanocompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 2A encapsulin nanocompartment shell
                     protein"
                     /protein_id="HNE00846.1"
                     /translation="MSDSSHVHSALGDHAARQLANATKTVPQLSTITPRWLVHLLQWV
                     PVEAGIFRLNKVSNPTSVEVACSQRDEAALPKTFVDYEEQPREYFLNAVTTVLDVHTR
                     VSDLYSSPHDQIREQLRLTIETIKERQESELINNPDYGLLANVADAQRLSTLNGAPTP
                     DDLDELITRVWKAPGFFLAHPLAIAAFGRECTRRGVPPATVSLFGCQFITWRGLPLIP
                     SDKIPVVDGRTQILLLRTGESRQGVIGLYQPGLTGEQSPGLSVRFMGIDRAAIASYLI
                     SLYCSLAILTDDAVAVLEDVEVGKFHEYAHTYQ"
     gene            complement(1918..2835)
                     /locus_tag="PLQ18_04000"
     CDS             complement(1918..2835)
                     /locus_tag="PLQ18_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020164530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HNE00847.1"
                     /translation="MNRRDDEVPVAPEAAQSWDIGPIVRELRAQRERWRTARQRRLTA
                     GGREFPARAAIGRILADLCGALFPMRLGPDDLQRESEDYYVGHTLDRALRALLGQVCL
                     ELGVASAEAVHHVRRFAEALPGIRALLDADVEAAFAGDPAARSVDEVLLCYPGVLAII
                     HHRLAHQLHGQGLALLARIVAEIAHAQTGIDIHPGARIGRSFFIDHGTGVVIGETAVI
                     GDHVRLYQAVTLGARSFQTAIDGSLEKGLARHPVVEDDVVIYAGATILGRITIGRGSV
                     IGGNVWLTHSVPPGSHVTQASTRQEQVNH"
     gene            3225..3356
                     /locus_tag="PLQ18_04005"
     CDS             3225..3356
                     /locus_tag="PLQ18_04005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE00848.1"
                     /translation="MNAILMNAILMNVIFMNPESSRINPDQAVRATGTGTLTPSPRL"
     gene            complement(3431..3907)
                     /locus_tag="PLQ18_04010"
     CDS             complement(3431..3907)
                     /locus_tag="PLQ18_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006749619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNE00849.1"
                     /translation="MTRALPELSAALIDAEAVLASARPAEPAGYAGGLSPVDAWTLFA
                     TGHAVLVDVRTVEELAYVGRVPGSLHVPWATGTAQTRNPRFLREFEKRVPQKDDVVLL
                     LCRSGKRSVAAAAALAAAGYTRAYNVLEGFEGERDADGHRGGQGGWRHHGLPWEQD"
     gene            complement(4305..>4584)
                     /locus_tag="PLQ18_04015"
     CDS             complement(4305..>4584)
                     /locus_tag="PLQ18_04015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE00850.1"
                     /translation="ASRQLTLHYALKPVLDALGASGVLSSLQIGARQARLHDDGLLVL
                     DPGVETRIEAALLRLLAGLPLAASLPTDALPPPPRALRRLPSAGRPAG"
BASE COUNT          744 a         1558 c         1582 g          700 t
ORIGIN      
        1 ccggcgccgg cgcatcgacg aagtagtacg gcccgtccgc atgcgtcggc cccggcgccg
       61 tcagcgagcg cagcagcgcc tgcagctcgg actcaccggg cagcccgaag ctctgcgccg
      121 gcagcacggc cggcgcactc gccgcggcgg cgagaccatc cagatcatcc agaccatccc
      181 gcccgccggc cgcacgcgga ccggccccgg cctcgtcgag gaagtagaac ggcgccgccg
      241 atgccgccac cggcgcggaa ccgaccagcg gcactccggc cccggtcgcc gcgaccggca
      301 ccgtcaccgg cggcacggca gccggcggcg gcgccggatg accgaccggc aacgaagtcg
      361 gcagcggcac cggcccgggc aaactggccg gcggctgcgc ggccgtcagc gacggcgcgc
      421 gctgcgcgag ccccggcagc gccgcggcca tggtctcgcc cggcagcatc gcgaagaagg
      481 cgctggcgag ctgcgtcagc ccggccacgt ccggcaggcc cggcggcgcg aggctgccgg
      541 gcagcgccgc cgcggcggcg gcctcactgg taggtgtggg catattcatg gaacttgccg
      601 acctccacgt cttcgagcac ggccaccgca tcgtcggtca ggatcgccag cgagcagtac
      661 agcgagatca ggtaggacgc gatcgcggcg cggtcgatgc ccatgaagcg caccgacaga
      721 cccgggctct gctcgccggt caggccgggc tgatacaggc cgatgacgcc ctgacggctc
      781 tcgccggtgc gcagcaggag gatctgcgtc ctgccgtcga cgaccggaat cttgtcggac
      841 gggatcagcg gcaggccgcg ccaggtgatg aactggcagc cgaacaggct gacggtcgcc
      901 ggcggcacgc cgcggcgcgt gcattcgcgg ccgaacgcgg cgatcgcgag cgggtgcgcg
      961 aggaagaagc ccggcgcctt ccagaccctg gtgatcagct cgtcgagatc gtccggagtc
     1021 ggcgcgccat tcagcgtcga gaggcgctgc gcatcggcga cgttggcgag caggccgtag
     1081 tcggggttgt tgatcagctc cgattcctga cgctccttga tcgtctcgat cgtcaggcgc
     1141 agctgctccc tgatctggtc gtgcggactg ctgtagagat ccgacacgcg cgtgtgcacg
     1201 tcgagcaccg tcgtcaccgc attcaggaaa tactcgcgcg gctgttcctc gtaatcgacg
     1261 aaggtcttcg gcagcgccgc ttcgtcgcgc tgcgagcagg cgacttcgac gctggtcgga
     1321 ttgctgacct tgttcagccg gaagatgccg gcctcgaccg gcacccactg cagcagatgc
     1381 accagccagc gcggcgtgat cgtcgaaagc tgcggtacgg tcttggtcgc attcgccagc
     1441 tggcgcgcgg catggtcgcc gagggcgctg tgcacgtggg agctgtcact catcttctga
     1501 atctccgctg ttgaaaaggg ccgctcatgc ctgaacggcg attcggacgg cgaccgctca
     1561 tgggtcgccg gaaaatgcag ggccggttgc cgatcacaat gctgctgtga accggccgga
     1621 ttttttcttc ctctcccctg cctttctcct gcttcgggag caaggcgctg taatgcggat
     1681 gccggatcag cggatcgcac cggccgaaaa cggcgggcac gaccgggccg gcgcgcgcag
     1741 ccatctgctg cgcacgtgaa tatgggggtg aaggctccgg cggcggatca ggcggccggg
     1801 tgctgccggt gtgtcatgcc gatcacacgt gaatgcggtg ttgatctccc tctcccccag
     1861 cccttccccg cgaaggggaa tgggctgaaa ggaccgggtg ttcagctgtg atggatatca
     1921 gtggttcacc tgttcctggc gcgtgctcgc ctgcgtgacg tggctgccgg gcggcacgct
     1981 gtgcgtgagc cagacgttgc cgccgatcac cgagccacgg ccgatcgtga tgcgcccgag
     2041 gatggtcgcg ccggcgtaga tgaccacgtc gtcctcgacc accgggtggc gggccagacc
     2101 tttttcgagg ctgccgtcga tcgcggtctg gaaactgcgg gcaccgagcg tgaccgcctg
     2161 atacagccgc acgtggtcgc cgatcaccgc ggtttcgccg atgacgacgc cggtgccgtg
     2221 gtcgatgaag aagctgcggc cgatgcgcgc ccccggatgg atgtcgatgc cggtctgcgc
     2281 gtgcgcgatc tcggcgacga tgcgcgcaag cagcgccaga ccctggccgt gcagctggtg
     2341 ggcgagccgg tggtggatga tcgccagcac gcccggatag cagagcagca cctcatcgac
     2401 gctgcgcgcc gccggatcgc cggcaaaggc ggcctcgaca tcggcatcga gcagcgcgcg
     2461 gatgccgggc agggcttcgg cgaagcggcg cacgtgatgc acggcctcgg ccgaggccac
     2521 gccgagttcg aggcagacct ggccgagcag cgcgcgcagg gcgcgatcca gcgtgtggcc
     2581 gacgtagtaa tcctcgctct cgcgctgcag gtcgtccggc ccgagccgca tcggaaacag
     2641 cgcgccgcag agatcggcga gaatgcgccc gatggcggcg cgggccggaa attcgcgacc
     2701 gccagccgtc agccggcgct ggcgcgccgt gcgccagcgt tcgcgctgcg cacgtaattc
     2761 gcgcacgatc gggccgatat cccaggactg cgcagcctcc ggcgcaacag gcacctcatc
     2821 gtcccggcgg ttcatgaaaa ctctccgtgc tgcccgaatg cagcgcatgg cgtttgaaac
     2881 aggccggagc ggtttccggc gctttccggc atccattcgg ccagacggac atgccgtccg
     2941 tatggcaatg attcgtctgc ctgcccaccg gcagacccgt gcggccgaaa cgccggtgct
     3001 tcacgaaaaa ccggatataa ccgcagacag cgtccagact ttctcacttg cccatgcgcc
     3061 tgccattcgt cctctttaca ggcggggcgg cagtataagg acaagctgtg cacagacaag
     3121 ccttccggca gtcatccggt tcaggccagt tccatgatcg cagacaggca tccgcaataa
     3181 cggacgcaat ccggatttac gctgccgaat taccggcatg tgaaatgaat gcgatcttga
     3241 tgaatgcgat cttgatgaat gtgatcttca tgaatccgga atccagccgg atcaatccgg
     3301 atcaggccgt ccgcgcgacc ggcaccggaa ctctgacgcc gtctccccgg ctgtaaatcg
     3361 gacgtgccac tcacggctgc attccgccac cgctcgcctc aggcgggtgc ggatgcagcc
     3421 tcgcgagcgt tcagtcctgc tcccagggca ggccatggtg ccgccagccg ccctgcccgc
     3481 cccgatggcc atcggcgtcg cgctcgccct cgaagccttc gagcacgttg taggcgcgcg
     3541 tgtagccggc cgcggccagc gcagccgcgg ccgccaccga gcgcttgccg ctgcgacaga
     3601 gcagcagcac gacatcgtcc ttctgcggca cgcgcttttc gaactcgcgc aggaagcgcg
     3661 ggttgcgcgt ctgcgccgtg ccggtcgccc acggcacgtg gaggctgccg ggcacgcgcc
     3721 cgacatacgc gagttcctcg accgtgcgca cgtcgacgag cacggcgtga ccggtggcga
     3781 acagcgtcca ggcatcgacc ggcgacaggc cgccggcgta gcccgcgggc tcggcgggac
     3841 gggcgctggc cagcacggct tcggcatcga tcagcgcagc gctcagttcg ggcagggcac
     3901 gggtcatgag gggcatctcc tcggcactcg ccgcgcctcg accgggcggg cgggtgtcca
     3961 tcacgggtcg gttgccggtc ggacgggtgc gtccgggcgg gtgatcgatg ccggaacgat
     4021 aagccgggtt ttatatttcc ataaataatg atttatgtat ttttaatttc ttttaggtat
     4081 aaaaagtctc gtctgcagcc atgcggaccg aaccggccag ccgctttccg gaatccttgt
     4141 ccatcggcat ctgcacgcgc ggcagccgcc ggccccggcc tcgcccggac agcgcggatg
     4201 ggtcaggacg acggcgaggc atggatgaac aagcccctcc cccgcgagca ggaaaaagcg
     4261 gaaaagccgg tgtgttgcgc cgggatcggc aggacagcgg tctttcagcc ggccggacgg
     4321 cccgcggacg gcaggcgccg cagcgcgcgc ggcggagggg gcagggcatc ggtcggcagc
     4381 gacgctgcca gcggcagacc ggcgagcagg cgcagcagcg cggcctcgat gcgggtctcg
     4441 acgcccggat cgagcaccag caggccgtca tcgtgcaggc gtgcctgccg cgctccgatc
     4501 tgcaggctgc tcagcacgcc gctggccccg agcgcgtcga gcaccggttt cagcgcgtaa
     4561 tgcagcgtca actgccggct ggcc
//
LOCUS       DAOQJH010000121         4555 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_11318_length_4555_cov_3.779333, whole genome shotgun sequence.
ACCESSION   DAOQJH010000121 DAOQJH010000000
VERSION     DAOQJH010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 4555)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4555)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4555
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11318_length_4555_cov_3.779333"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..787)
                     /locus_tag="PLQ18_04020"
     CDS             complement(<1..787)
                     /locus_tag="PLQ18_04020"
                     /inference="COORDINATES: protein motif:HMM:NF019236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NosD domain-containing protein"
                     /protein_id="HNE00851.1"
                     /translation="MKRSADPLWLLAVLVCAGVLLAIGDALAATATATGRRVSPADGP
                     LQAAIDAAAPGEVLRLAPGVYAGGVKIDRPLTLAGEPGAVIDGGGQGDVVRVTAPDVT
                     LSGLGLRNSGRELGDMNSGVFLQKGADRARVEGCTIEAVAFGIWLDALDGARILNNRI
                     TGDAALRSQDRGNGIHFFSTRHSLAAGNEVGEARDGIYIDTSDDNELRDNFLHDLRYG
                     IHYMYANRNALLGNRTTRTRTGYALMSSKNLKVIGNRSERDENY"
     gene            complement(857..2785)
                     /gene="nosZ"
                     /locus_tag="PLQ18_04025"
     CDS             complement(857..2785)
                     /gene="nosZ"
                     /locus_tag="PLQ18_04025"
                     /EC_number="1.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007349527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050304 - nitrous-oxide reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019333 - denitrification pathway [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAT-dependent nitrous-oxide reductase"
                     /protein_id="HNE00852.1"
                     /translation="MSEQHDPQGHGETPAGSSAAHGLTRRGFLGAAAAVGAAGATGVA
                     GALLTAQSRPAGAATAAEDPSLKAHVAPGELDEYYGFWSGGHSGEVRVLGIPSMRELM
                     RIPVFNTCSATGWGLTNESKHVLGDGARFHNGDAHHPHVSMTDGRYDGKYLFINDKAN
                     TRVARIRLDIFKCDRITSIPNVSAIHGLRLQKVPHTKYVFANAEMPIPQPNDGRDLDN
                     AQNYWTMFNAIDAESMEVAWQVIVDGNLDNTDADYTGRYAASTCYNSERGVHLGDMMR
                     NERDWVVVYNIPAIEAAIQAGKATTIGDSKVPVLDGRKAANLGITVYIPVPKNPHGLN
                     TSPDGKYFIANGKLSPTVSIIDIGKLDDLFAGKIEPRKAIAAEVELGLGPLHTTFDGR
                     GNAYTTLFLDSQVCKWNIADAIRAWNGEKVDYIRQKLDVSYQPGHNHASLTESREADG
                     KWLAVLCKFSKDRFLNTGPLKPENDQLIDISGEEMKLVHDGPTFAEPHDCIMVRRDQL
                     RPVDVWKRDDPFFAITRERAKKDGITLETDNKVIRDGRQVRVYMTSAAPTYGIQEFTV
                     KQGDEVTVTITNLDTVSDLTHGFCVVNHGVSMEIGPQQTASISFVADKPGVHWYYCNW
                     FCHAMHMEMVGRMLVEKA"
     gene            complement(2987..>4555)
                     /locus_tag="PLQ18_04030"
     CDS             complement(2987..>4555)
                     /locus_tag="PLQ18_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003124291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNE00853.1"
                     /translation="DRAVDHLDQVRQLDEPFVELAWAYLNPPTVGRSLLGDAAYARLM
                     AGLAPGEHAIAVLGNGYSFKGSGYVRGGIFDRVQLRQNDQAISFRDSDFQRLSDVYAP
                     GMPRFREMVIFFIREHYHFDPGTPWQLELLVRRQTGPLDSVFTSFSGGYETPARYLEL
                     PAAPLPAPAAPGSSTTGAPQAAVTAAADATALAPMEPIWKAVWRERGFRIAVLGAGLT
                     LLTLILLFQDVLVTRPRLLPWLRNGFLLWTLLFIGWYGLAQLSVVNVLTFTNALIAGF
                     RWDTFLIDPMMFILWGFVAATLLLWGRGVYCGWLCPFGALQELVNELALKLGIRQWRL
                     PFAVHERLWAVKYLILLGLFAVSLQSLGEAEQLAEIEPFKTAITLRFVREWGFVAWAL
                     GLVLVSAVNRKFYCRYLCPLGAALAIPARLRLFDWLKRRRECGKPCQICAEECEVQAI
                     HPTGQINANECHYCLDCQVTYFDDGKCPPLVDKRRKRDKAAALAAGREVGREAGAPAA
                     AFDGPRRFVRPPAP"
BASE COUNT          686 a         1543 c         1594 g          732 t
ORIGIN      
        1 cgtagttctc gtcgcgctcg gagcggttgc cgatgacctt caggttcttc gaagacatca
       61 gcgcgtagcc ggtgcgcgtg cgcgtcgtgc ggttgccgag cagcgcgttg cggttggcgt
      121 acatgtaatg aatgccgtag cgcaggtcgt gcaggaagtt gtcgcgcagc tcgttgtcgt
      181 cgctggtgtc gatgtagatg ccgtcgcgcg cctcgccgac ctcgttgccg gcggcgagcg
      241 aatggcgggt gctgaagaag tggatgccgt tgccgcgatc ctgcgagcgc agcgcggcat
      301 cgccggtgat gcggttgttc aggatccgcg cgccgtcgag cgcatcgagc cagatgccga
      361 aggcgaccgc ctcgatcgtg cagccctcga ctcgcgcccg gtcggcgccc ttctgcagga
      421 acacgcccga gttcatgtcg ccgagttcgc gcccggagtt gcgcagcccg aggccgctca
      481 gcgtcacatc gggcgccgtg acccgcacga catcgccctg cccgccgccg tcgatgacgg
      541 cgccgggctc gccggcgagc gtcagcggcc ggtcgatctt cacgccgccg gcgtagacgc
      601 cgggcgcaag ccgcagcacc tcaccgggcg ccgccgcgtc gatcgccgcc tgcagcggcc
      661 cgtcggccgg cgacacgcgt cgtccggtgg cggtggcggt ggcggcgagt gcatcgccga
      721 tcgccagcag cacgccggca cagaccagca cggccagcag ccacaggggg tccgcagatc
      781 gcttcatgtc catccctccc gccggctgcc gcagccgcac ggcgtgcggc tgcggaccgg
      841 ctgcggcgcg cctgcctcag gccttctcga ccagcatccg cccgaccatc tccatgtgca
      901 tcgcgtggca gaaccagttg cagtagtacc agtgcacgcc gggcttgtcg gcgacgaagc
      961 tgatcgacgc ggtctgctgc gggccgatct ccatcgacac gccgtggttg acgacgcaga
     1021 agccgtgcgt gagatccgag acggtgtcga ggttggtgat cgtcaccgtc acctcgtcgc
     1081 cctgcttcac cgtgaactcc tggatgccgt aggtcggcgc cgccgaggtc atgtagacgc
     1141 gcacctgccg gccgtcgcgg atgaccttgt tgtcggtttc gagcgtgatg ccgtccttct
     1201 tcgcacgctc gcgcgtgatg gcgaagaacg gatcgtcgcg cttccagaca tcgacgggcc
     1261 ggagctggtc gcggcgcacc atgatgcagt cgtgcggctc ggcgaaggtc gggccgtcgt
     1321 gcacgagctt catctcctcg ccggagatgt cgatcagctg gtcgttctcg ggcttcagcg
     1381 gcccggtgtt caggaagcga tccttcgaga acttgcagag caccgccagc cacttgccgt
     1441 cggcctcgcg cgattcggtc aggctggcgt ggttgtggcc gggctgatag ctcacgtcga
     1501 gcttctggcg gatgtagtcg accttctcgc cgttccaggc gcggatcgcg tcggcgatgt
     1561 tccacttgca gacctggctg tcgaggaaca gcgtcgtgta ggcgttgccg cggccgtcga
     1621 aggtcgtgtg cagcgggccg agtccgagtt cgacctcggc ggcaatggcc ttgcgcggct
     1681 cgatcttgcc ggcgaacaga tcatcgagct tgccgatgtc gatgatcgac acggtcggcg
     1741 acagcttgcc gttggcgatg aagtacttgc cgtccggcga ggtgttgagg ccgtgcgggt
     1801 tcttcggcac cgggatgtaa accgtgatgc cgaggttcgc ggccttgcgc ccgtcgagca
     1861 ccggcacctt cgaatcgccg atcgtcgttg ccttgccggc ctggatcgcc gcctcgatcg
     1921 ccgggatgtt gtagacgacc acccagtcgc gctcgttgcg catcatgtcg ccgagatgca
     1981 cgccgcgctc ggagttgtaa caggtcgagg ctgcgtacct gccggtgtag tcggcatcgg
     2041 tgttgtcgag gttgccatcg acgatgacct gccaggcgac ctccatgctc tcggcgtcga
     2101 tggcgttgaa catggtccag tagttctgcg cgttgtcgag atcacgcccg tcgttcggct
     2161 gcgggatcgg catctcggcg ttcgcgaaca cgtacttggt gtgcggcacc ttctgcaggc
     2221 gcaggccgtg gatcgccgag acgttcggga tcgacgtgat cctgtcgcac ttgaagatgt
     2281 cgaggcggat gcgcgcgacg cgggtgttgg ccttgtcgtt gatgaacagg tacttgccgt
     2341 cgtagcggcc gtcggtcatc gacacgtgcg gatgatgcgc gtcgccgttg tggaagcgcg
     2401 cgccgtcgcc gagcacgtgt ttgctctcgt tggtcaggcc ccagcccgtg gccgagcagg
     2461 tgttgaacac cgggatgcgc atcagctcgc gcatcgacgg gatgccgagc acgcgcacct
     2521 cgccggaatg cccgccggac cagaagccgt agtactcgtc gagctcgccc ggcgcgacgt
     2581 gcgccttcag cgacgggtct tcggcagcgg tcgccgcccc ggccgggcgc gactgcgccg
     2641 tcagcagcgc cccggcaacg ccggtcgcgc cggcggcgcc gacggccgcc gcggcgccga
     2701 gaaagccgcg gcgcgtcagc ccgtgcgcag cactgctgcc ggccggcgtt tccccgtggc
     2761 cctgcgggtc gtgttgctcg ctcatggtga tccgtcctca gaagattgca ctgtgaatgc
     2821 cgccggcgac ccgcgggcgc agaccgcccc cgggttcgtt ccgaacggcg gattcggcgc
     2881 cgcgtccgcc ggaactccgg cagcgatgcg acggcgaggg aaaaaagcgc gggtgctgac
     2941 ccgccggcga agggcgtgaa gcaggcatgg cggcggaccc ggtggctcag ggcgccggcg
     3001 ggcgcacgaa gcggcgcggc ccgtcgaagg ccgcggccgg ggcgccggcc tcgcgaccga
     3061 cctcacggcc ggcggcgagt gccgcggcct tgtcgcgctt cctgcgcttg tcgacgagcg
     3121 gcggacactt gccgtcgtcg aagtacgtga cctggcagtc gaggcagtaa tggcactcgt
     3181 tggcgttgat ctgcccggtc gggtggatcg cctgcacctc gcattcctcg gcgcagatct
     3241 ggcacggctt gccgcactcg cgccggcgct tcagccagtc gaacagccgc agccgcgccg
     3301 ggatcgccag cgccgcgccg agcgggcaca ggtagcggca gtagaacttg cggttgacgg
     3361 ccgagacgag gaccagaccc agcgcccagg cgacgaagcc ccactcgcgc acgaagcgca
     3421 gcgtgatcgc ggtcttgaac ggctcgatct cggcgagctg ctcggcctcg ccgagcgact
     3481 gcagcgacac cgcgaacagc ccgagcagga tcaggtactt caccgcccac agccgctcgt
     3541 gcaccgcaaa cggcagccgc cactggcgga tgccgagctt cagcgccagc tcgttgacga
     3601 gttcctgcag cgcgccgaag gggcagagcc agccgcagta gacgccgcgt ccccacagca
     3661 gcagcgtcgc ggcgacgaag ccccagagga tgaacatcat cggatcgatg agaaacgtat
     3721 cccaccggaa gccggcgatc agcgcgttcg tgaaggtcag cacgttgacg accgacagct
     3781 gcgcgagccc gtaccagccg atgaacagca gcgtccagag caggaagccg ttgcgaagcc
     3841 acggcagcag ccgcggccgc gtcaccagca cgtcctggaa cagcaggatc agcgtcagca
     3901 gcgtcaggcc cgcgccgagc accgcgatcc ggaaaccgcg ctcgcgccac accgccttcc
     3961 agatcggctc catcggcgcc agcgcggtcg catccgccgc ggccgtcacc gcggcctgcg
     4021 gcgcacctgt ggtgctgctg ccgggggcgg ccggcgccgg cagcggcgcg gcgggcagct
     4081 ccagatagcg cgccggcgtc tcgtagccgc cgctgaagct cgtgaacacc gaatcgagcg
     4141 gcccggtctg gcggcgcacc agcagttcga gctgccacgg cgtgcccgga tcgaagtggt
     4201 aatgctcgcg gatgaagaag atcaccatct cgcgaaagcg cggcatgccg ggcgcgtaga
     4261 catcgctcag gcgctggaag tcgctgtcgc ggaagctgat cgcctggtcg ttctggcgca
     4321 gctgcacgcg atcgaagatg ccgccgcgca cgtagcccga gcccttgaac gaatagccgt
     4381 tgcccagcac cgcgatcgcg tgctcgccgg gcgcaagccc cgccatcagc cgtgcatacg
     4441 cggcatcgcc gagcaggctg cgcccgacgg tcggcgggtt caggtaggcc caggccagtt
     4501 cgacgaatgg ctcgtcgagc tggcggacct ggtcgaggtg gtctacagcc cgatc
//
LOCUS       DAOQJH010000122         4550 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_11337_length_4550_cov_3.994661, whole genome shotgun sequence.
ACCESSION   DAOQJH010000122 DAOQJH010000000
VERSION     DAOQJH010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 4550)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4550)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4550
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11337_length_4550_cov_3.994661"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1398)
                     /locus_tag="PLQ18_04035"
     CDS             complement(<1..1398)
                     /locus_tag="PLQ18_04035"
                     /inference="COORDINATES: protein motif:HMM:NF003715.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HNE00854.1"
                     /translation="MQPSDRLILLAGLLMLVSVITTRLSRRIGMPILLVFLGFGMLAG
                     EDGPGGLAWSDVPTSHFIGSLALAVILFDGGMRTQLKSFRVGLRPALGLATLGVLFSA
                     AITGLFAAWALGFDYVEGLLIGAIVGSTDASAVFGVLHAHGLRLKERVGATLEIESGL
                     NDPMAIFLTVAVLEWLLAGRPALDAAPLLLFAQQMGVGAVLGLAGGYLVWLLLDRLDL
                     GAVFTPLFAFAAGLALYGTTAFFGGSGFLAVYLGGLVIGNRPSVGLSLVLRLHEGLAW
                     LAQIAMFLILGLLVTPSQIVPVAWPALLVGLGLMFAARPLAVWLCLLPFRFSPGERLF
                     IAWVGLRGAVPIVLAMFPLLADLPHAGTMFNVAFVVVLLSLSVQGWTVAPLARRLGLE
                     LPPAVTPQRHVVSELPGTPGAVFASYVVAVNAPVAGGVLGTLGLPPASRPVAVARGGR
                     LLAEPASVRLVAGDAV"
     gene            complement(1589..2206)
                     /locus_tag="PLQ18_04040"
     CDS             complement(1589..2206)
                     /locus_tag="PLQ18_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017840781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HNE00855.1"
                     /translation="MNAVVCPRLTVADYLAGERDAELRHEYENGFVRAMTGTSRAHNS
                     IAMNVAAVLHSHLRGSPCRVHVSDVKVHVDLADDQRFYYPDVLVDCGSGGDAYVASAP
                     KLVVEVLSASTERRDRAEKAQGYRRLPTLAEYVLIAQDVRRVEVYRRSEGWEFERTID
                     SAATPVRFESLGLELTLGAIYEGLELPADPGDSPKPVHGSPGSAG"
     gene            complement(2378..2803)
                     /locus_tag="PLQ18_04045"
     CDS             complement(2378..2803)
                     /locus_tag="PLQ18_04045"
                     /inference="COORDINATES: protein motif:HMM:NF019460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1636 domain-containing protein"
                     /protein_id="HNE00856.1"
                     /translation="MPSTLFVCTTCRRADNVDDATTESCGSKLKRELEAAAESLPEAA
                     RPKIEGYECLMNCERACNVAIRAQGKSGFLLGGFAPVLADARALLDYTWLYNASPGGV
                     VPYRDWPDGVRGRFCGRIPAASADPLPQARSEPALEGEK"
     gene            complement(3085..3615)
                     /locus_tag="PLQ18_04050"
     CDS             complement(3085..3615)
                     /locus_tag="PLQ18_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HNE00857.1"
                     /translation="MPAPESVGVSEVLRLRAEPLTQAAFAPFGDVIEPAAASRHYPIN
                     AGSAERFHDVMRIDTGTADGHTVLSLVFGQPVTLPARLRLLERHPLGSQSWLPLDGQR
                     FLIVVAPAGEQWALADVRVFVSDGRQGVNYARGVWHHPLLSLGETPGRYVVVDRDGPG
                     DNCEERPLPRVVELVI"
     gene            complement(3615..>4550)
                     /gene="alc"
                     /locus_tag="PLQ18_04055"
     CDS             complement(3615..>4550)
                     /gene="alc"
                     /locus_tag="PLQ18_04055"
                     /EC_number="3.5.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007743818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004037 - allantoicase activity [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoicase"
                     /protein_id="HNE00858.1"
                     /translation="RALTVSDEWFAPRERLLDDAEAVFIPGKYDDHGKWMDGWETRRK
                     RVTGHDWCIVRLAVPGTVYGVDIDTSHFTGNFPPAASIDGCCLAEGGDPDADTVWQPL
                     LAAVSLGGDAHHFHVLDARLPVTHVRLNIFPDGGVARLRVHGRALAGDTAPGAELELS
                     SMLLGGRIVACNNSHYGPPHALLAPGRGVNMGDGWETRRRREPGNDWVIVELARPGRI
                     TRIEVDTAHFKGNYPDACSLQAACAGAGTDQSMITQSMFWPELLPAQKLSADAIHHFD
                     AEALAALGPVTHVRFNIHPDGGVSRLRVFGRVESV"
BASE COUNT          761 a         1629 c         1595 g          565 t
ORIGIN      
        1 cacggcatcg ccggcgacga ggcgcacgct cgccggttcg gccagcagcc ggccgccgcg
       61 ggccaccgcc accggccggc tggccggcgg caggccgagc gtgccgagca caccaccggc
      121 gaccggcgcg ttgacggcga cgacgtagct cgcgaacacc gcgcccggcg tgcccggcag
      181 ctccgagacc acatggcgct gcggcgtcac cgccggcggc agctccagtc cgagccggcg
      241 cgccagcggt gcaaccgtcc agccctgcac cgacagcgac agcagcacga cgacgaaggc
      301 gacgttgaac atcgtgccgg catgcggcag gtcggcgagc agcgggaaca tcgccagcac
      361 gatcggcacc gccccgcgca gccccaccca ggcgatgaac agccgctcgc ccggcgagaa
      421 gcggaagggc agcaggcaga gccataccgc cagcggccgg gcggcgaaca tcagcccgag
      481 gccgacgagg agcgccggcc acgccaccgg cacgatctgc gacggcgtca ccagcaggcc
      541 gaggatcagg aacatcgcga tctgcgccag ccacgcgagc ccctcgtgca ggcgcagcac
      601 cagcgacagg ccgaccgaag gccggttgcc gatcaccagc ccgccgagat agacggcgag
      661 gaagccgctg ccgccaaaga acgccgtcgt gccgtacagc gccagcccgg ccgcgaacgc
      721 aaagagcggc gtgaacaccg cgccgagatc gagccggtcg agcagcagcc agaccagata
      781 accgccggcg agcccgagca cggcgccgac gcccatctgc tgcgcgaaca gcagcagcgg
      841 cgcggcatcg agggccggcc ggccggcgag cagccattcg agcacggcga cggtcaggaa
      901 gatcgccatc gggtcgttga ggccggattc gatctccagc gtcgcgccga cgcgctcctt
      961 cagacgcagg ccgtgcgcgt gcagcacgcc gaacaccgcc gaggcgtcgg tcgagccgac
     1021 gatcgcgccg atcagcaggc cctcgacata gtcgaagccg agcgcccagg cggcgaacag
     1081 gccggtgatc gcggcactga acagcacgcc gagcgtcgcc agcccgagcg ccgggcgcag
     1141 gccgacgcgg aagctcttga gctgcgtgcg catgccgccg tcgaacagga tcacggcgag
     1201 cgccagactg ccgatgaagt ggctggtcgg cacgtcgctc caggccaggc cgccggggcc
     1261 gtcctcgccg gcgagcatgc cgaagccgag gaacaccagc aggatcggca tgccgatcct
     1321 ccggctcagc cgggtggtga tgacgctgac cagcatcagc aggccagcga gcaggatcag
     1381 gcgatcgctc ggttgcacgg gtcgattccg cagaaggagg gaaacgggcg ccgacccccg
     1441 cgttcaggcg ccggccatct gcgggtgcag catacggaag accgtgcggc gcgccggtga
     1501 cgcgccactc attaaattat ggacagaacc ccggacaggc cctgcaacgg cccgccggca
     1561 tcctcgccgg cgccggcgcg cgccgcactc agccggcgga ccccgggctg ccgtgcacgg
     1621 gcttcggcga gtctcccgga tcggccggca gctccaggcc ctcgtagatc gcaccgagcg
     1681 tcagttcgag accgaggctt tcgaagcgca ccggcgtggc ggcgctgtcg atcgtgcgct
     1741 cgaactccca gccttcgctg cgccggtaga cctcgacgcg gcgcacgtcc tgcgcgatca
     1801 gcacgtactc ggcgagcgtc ggcagccgcc ggtagccctg ggccttctcg gcacggtcac
     1861 gccgctcggt cgaggccgac agcacctcga cgaccagctt cggtgccgac gccacataag
     1921 catcaccgcc gctgccgcag tcgacgagca cgtccgggta atagaagcgc tggtcgtcgg
     1981 cgagatcgac gtggaccttg acgtcggaga catgcacacg acacggcgag ccgcgcagat
     2041 gactgtgcag taccgccgcg acattcatgg cgatgctgtt gtgcgcccgg ctggtgccgg
     2101 tcatcgcccg cacgaagccg ttctcgtact catgccggag ttcggcatcg cgctcgccgg
     2161 cgaggtaatc ggcgaccgtc aggcggggac agacgactgc gttcatggcg atggacctct
     2221 gctgccggac gcgactggcc tgcagtctcg acgccgatgc ccgcagaggc aagcctgcag
     2281 atcggaaaga aaaaacccgc tgcacggaca gcgggttgcg atcaggagcg tgaggccact
     2341 cccggattca cggacgccgg ccggcgccgg ccgctgccta cttctcgcct tccagcgccg
     2401 gctcggagcg ggcctgcggc agcggatcgg cgctcgcggc cgggatgcga ccgcagaagc
     2461 ggccgcgcac gccatccggc cagtcgcgat agggcacgac accgcccggc gaggcgttgt
     2521 acagccacgt gtagtcgagc agcgcgcgcg catcggcgag caccggcgcg aagccgccga
     2581 gcaggaagcc gctcttgccc tgcgcgcgga tcgcgacgtt gcaggcgcgc tcgcagttca
     2641 tcaggcattc atagccttcg atcttcggac gggcggcctc gggcaggctc tcggctgcgg
     2701 cttccagctc gcgcttgagc ttgctgccgc aggactcggt ggtcgcatca tcgacgttgt
     2761 cggcgcgacg gcaggtggtg cagacgaaca gcgtggacgg catcagcaca cctccgcaca
     2821 acggcgtgcc ggacgcaaac agaccctgag cttgcgcatc gctggaaccc tcttcccgtg
     2881 cttgacgttt atcgttgttg gaatgcagcc ggattcgctg agaccggcgc agccgcactg
     2941 gacggacgca tcagccgcgg cggccgggtg gcgtccgtgg ccgacggaag catgccgggc
     3001 tggtgcgtgc cggagtatcc acgtccggcg cgccgcagca agcccccgcg ctgcgctgcc
     3061 gcagcgcgcg cagggtgaaa tgcctcagat cacgagttcc accactcgcg gcagcgggcg
     3121 ttcctcgcag ttgtcgccgg gaccgtcgcg atcgacgacg acgtagcggc ccggcgtctc
     3181 accgagcgac agcagcgggt gatgccagac gccgcgcgcg tagttgacgc cctggcggcc
     3241 gtcgctgacg aacacgcgca cgtccgcgag cgcccactgc tcgccggccg gcgcgaccac
     3301 gatcaggaag cgctggccat cgagcggcag ccagctctgg ctgccgagcg gatggcgttc
     3361 gagcagcctg agcctggccg gcagcgtcac cggctggccg aagaccaggc tcagcaccgt
     3421 gtgcccgtct gcggtgccgg tgtcgatgcg catgacatcg tggaagcgct cggcgctgcc
     3481 ggcgttgatc ggatagtgcc ggctggcagc ggccggctcg atcacgtcgc cgaacggcgc
     3541 gaacgccgcc tgcgtcagcg gctcggcgcg caggcgcagc acttcggaaa ccccgacgga
     3601 ctccggagcc ggcatcacac cgactccacg cgcccgaaca cgcgcaggcg gctgacgccg
     3661 ccgtccggat ggatgttgaa gcgcacgtgc gtgaccgggc cgagcgcggc cagggcttcg
     3721 gcatcgaagt gatggatcgc gtcggccgac agcttctgtg ccggcagcag ctcgggccag
     3781 aacatcgact gcgtgatcat cgactggtcg gtgcctgccc ctgcgcaggc ggcctgcagc
     3841 gagcaggcgt ccgggtaatt gcccttgaag tgcgcggtat cgacctcgat gcgcgtgatc
     3901 cgcccgggcc gcgccagctc gacgatgacc cagtcgttgc cgggctcgcg ccgccggcgc
     3961 gtttcccagc catcgcccat gttgacgccg cgccccggcg cgagcagcgc atgcggcggg
     4021 ccgtagtggc tgttgttgca ggcgacgatg cgcccgccga gcagcatcga cgacagctcc
     4081 agctccgccc ccggcgccgt gtcgccggca agcgcgcggc cgtgcacgcg cagccgcgcc
     4141 acgccgccat ccgggaagat gttcaggcgc acatgcgtga ccggcaggcg ggcgtcgagc
     4201 acgtggaagt gatgcgcatc gccaccgagc gacacggccg cgagcagtgg ctgccagacg
     4261 gtatcggcat ccggatcgcc gccctcggcg aggcagcagc cgtcgatcga cgccgccggc
     4321 gggaagttgc cggtgaagtg cgaggtgtcg atgtcgacgc cgtacaccgt gcccggcacg
     4381 gccagacgca cgatgcacca gtcatggccg gtgacgcgct tgcggcgcgt ctcccagccg
     4441 tccatccatt tgccgtgatc gtcgtacttg ccgggaatga acacggcctc ggcatcgtcg
     4501 agcaggcgct cgcgcggtgc gaaccattca tcggacacgg tcagggctct
//
LOCUS       DAOQJH010000123         4534 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_11402_length_4534_cov_3.947979, whole genome shotgun sequence.
ACCESSION   DAOQJH010000123 DAOQJH010000000
VERSION     DAOQJH010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 4534)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4534)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4534
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11402_length_4534_cov_3.947979"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(63..1118)
                     /locus_tag="PLQ18_04060"
     CDS             complement(63..1118)
                     /locus_tag="PLQ18_04060"
                     /inference="COORDINATES: protein motif:HMM:NF027400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilW family protein"
                     /protein_id="HNE00859.1"
                     /translation="MGELPDNHRLLNARAPRCAAGGFTLIEMMLALALGLLIVAGALA
                     VYAANRAVYQHQEAWARLQENARLAFELMARDGRQAGFMACLPVAAPLTNVLNSATTY
                     EWLFEQPVDGFEASGVSWSPTLPSGFPAPYRGTDVLTLRGAQSSPQTRVSAQASTSAD
                     VTLDNGSGFASGDFVLLAAADCSEGAIFQVSGVTAGAAPVASLAHAAGAGSPGNASAD
                     LGARYVGGLALKLTTYSYYVAAGSDGRPALYRRQGSAMPVELIPGVDDLQVLYGLADG
                     SNGDYASRYATAAVISADADNWQKIVGLRVTMTLESPDDGITDSAQAVTINGVSGSDR
                     RLRRTFTATIAIRNRLP"
     gene            complement(1085..1540)
                     /gene="pilV"
                     /locus_tag="PLQ18_04065"
     CDS             complement(1085..1540)
                     /gene="pilV"
                     /locus_tag="PLQ18_04065"
                     /inference="COORDINATES: protein motif:HMM:TIGR02523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus modification protein PilV"
                     /protein_id="HNE00860.1"
                     /translation="MDGRRQVQGFTLIEVLVALLIAAIGLLGMAALHLSGLRSVETAY
                     QRSLATLAANDLLERMRANADAVRAGNYASAYGAAATASTDCEATSCSGAQMAAFDLA
                     RWKASLGALPAGDGKVEMSGTRVTVTVRWDHSRSGDGTQWASFLITTDF"
     gene            complement(1622..2191)
                     /locus_tag="PLQ18_04070"
     CDS             complement(1622..2191)
                     /locus_tag="PLQ18_04070"
                     /inference="COORDINATES: protein motif:HMM:NF023444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HNE00861.1"
                     /translation="MNATHCPRIRQDGFTLVELMVVLSVAAILTTIGVPSFLSTIRDN
                     GVSTRTNELITDLALARTEAIRRGQTVTVCPSTDGTSCAGSGGWEQGWISFRDNDASG
                     VLDSSDCSTPADCVLRRSPALASGYTLRANNVTLAAHLSFDGIGMATASFGQLRLCDE
                     RGISHGRIIDVAAAGRAVAKQPSGSDSCE"
     gene            complement(3240..3908)
                     /locus_tag="PLQ18_04075"
     CDS             complement(3240..3908)
                     /locus_tag="PLQ18_04075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE00862.1"
                     /translation="MRRPGLWIGAAALVLGVLTVPLFAPGSKPSVPGTDLPWQVETDA
                     HGVPAVFGIRLGETRLQDVLPRLGRSVELGLFRQADGRSVLEAYYREIELGGLTTKLI
                     LRLGAGQEALDAMIARAGKGDPTPSGSMQYIPASTDDHAIIALPIIGLTYSPLVRLDE
                     DVIRSRFGEPAEKLAEKDGVHWLYPARSLDLFRQDNGKSILTYLLPADYAQWRAQYRP
                     PSGQ"
     gene            complement(3908..>4534)
                     /gene="hemB"
                     /locus_tag="PLQ18_04080"
     CDS             complement(3908..>4534)
                     /gene="hemB"
                     /locus_tag="PLQ18_04080"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018954831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HNE00863.1"
                     /translation="LDPYTSHGQDGLVDADGYVMNDETVEVLVKQALSHAEAGADVVA
                     PSDMMDGRIGAIRNALEAAGHIHTRILAYSAKYASSFYGPFRDAVGSAGALGKGNKYT
                     YQMDPANSDEALREVALDLDEGADMVMVKPGMPYLDIVRRVKSEFGVPTFVYQVSGEY
                     AMQMAAIQNGWLAEAVIAESLLCIKRAGADAVLTYFAKRVAADLRERG"
BASE COUNT          724 a         1579 c         1509 g          722 t
ORIGIN      
        1 ggcaccgcgc tcgcggcgcg gcgcgcgcag cgaagccggg gagcggaaaa acggagtggc
       61 gttcatggca ggcggttgcg gatcgcgatc gtggccgtga aggtccggcg caggcggcgg
      121 tcgctgccgc tgacaccgtt gatggtcacg gcctgcgcgc tgtcggtgat gccgtcgtcc
      181 gggctttcca gcgtcatcgt cacgcgcagg ccgacgatct tctgccagtt atcggcatcg
      241 gcgctgatca ctgcagccgt cgcgtagcgg ctggcgtagt cgccgttgct gccgtcggcg
      301 aggccgtaga gcacctgcag gtcatcgacg cccgggatca gctcgaccgg catcgcgctg
      361 ccctggcggc ggtacagcgc cgggcggccg tcgctgccgg cggcgacgta gtacgaatac
      421 gtggtcagct tcagcgccag tccgccgacg tagcgcgcgc cgagatcggc actggcgttg
      481 ccgggcgaac cggcgccggc ggcgtgcgcg agcgaggcga ccggcgcggc cccggccgtg
      541 acgccgctca cctggaagat cgcgccttcc gagcagtcgg ccgccgccag cagcacgaag
      601 tcgccgctcg cgaagccgct gccgttgtcg agtgtcacgt cggcggaggt gctggcctgg
      661 gcgctgacgc gcgtctgcgg cgaggactgc gcaccgcgca gcgtcagcac gtcggtgccg
      721 cggtacggcg ccggaaagcc tgaaggcagc gtcggtgacc acgacacgcc gctcgcctcg
      781 aagccgtcga ccggctgttc gaacagccat tcgtaggtcg tcgcgctgtt cagcacgttg
      841 gtgagcgggg cggccaccgg caggcaggcc atgaaaccgg cctgacggcc gtcgcgggcc
      901 atcagctcga aggccagccg cgcgttctcc tgcagccgcg cccaggcttc ctgatgctgg
      961 tagacggcgc ggttggcagc gtagacggcg agcgccccgg cgacgatcag cagcccgagc
     1021 gccagcgcga gcatcatctc gatcagcgtg aaaccgccgg cggcgcagcg tggcgcgcgc
     1081 gcgttcagaa gtcggtggtt atcaggaagc tcgcccattg cgtcccgtcc cccgatcgcg
     1141 aatggtccca gcgcaccgtg acggtgacgc gcgtgccgct catctcgacc ttgccgtcgc
     1201 cggccggcag cgcgccgagc gaagccttcc agcgcgccag gtcgaaggcc gccatctgtg
     1261 cgccggagca gctcgtcgcc tcgcagtcgg tcgaggcggt ggcggccgcg ccgtaagcgc
     1321 ttgcgtagtt gccggcgcgc accgcatcgg cgttggcgcg catgcgctcc agcaggtcgt
     1381 tggcggcgag cgtcgccagg ctgcgctggt aggcggtctc gacgctgcgc agccccgaaa
     1441 gatgcagcgc cgccatgccg agcaggccga tcgccgcgat cagcagcgcg accagcacct
     1501 cgatcagcgt gaatccctgc acctgccgcc tgccgtccac gcccgcgtcc cccgtccccg
     1561 atgcggacgc cggacctgcc ggcccggcga cgcgcctgcc gtcatcgccg gcaccggctc
     1621 atcactcgca gctgtcgctg ccggaaggct gcttcgccac cgcccggccg gcggcagcca
     1681 cgtcgatgat gcggccgtgg ctgatgccgc gctcgtcgca gagccgcagc tggccgaagc
     1741 tcgcggtcgc catgccgatg ccgtcgaagc tcaggtgcgc agcgagcgtg acgttgttcg
     1801 cgcgcagcgt gtagccgctc gcgagcgccg ggctcctgcg cagcacgcag tcggccggcg
     1861 tcgagcagtc gctgctgtcg agcacgccgg aagcgtcgtt gtcccggaag ctgatccagc
     1921 cctgctccca gccgccgctg ccggcgcagc tcgtgccgtc ggtgctcggg cagacggtga
     1981 cggtctggcc gcggcggatc gcctcggtgc gtgccagcgc cagatcggtg atcagctcgt
     2041 tggtccgggt gctgacgccg ttgtcgcgga tcgtcgacag gaaggaagga acaccgatcg
     2101 tggtcaggat cgccgccacg gacagcacga ccatcagttc gaccagcgtg aagccgtcct
     2161 ggcggatgcg ggggcagtgc gtggcgttca tttcattgtt tcgggcgggt ttgccgccgc
     2221 gatcctcgtt atgggtcagg tgccgttccg gttcgtcgaa atcttgacac ccgatactga
     2281 gcatcaaatc cgggcgtcga atcgtcgaaa gtccgtcacc cgaaacatta actaaaggat
     2341 aatctgcctg atctccctgg cggaatccag tgtcacagcg ttttacagcc gctgcagccg
     2401 atgcgcgctt cccgctggca tccatcggct ttccgaccct gtcagcatgc gtaacgaaac
     2461 gatcttcagc caatcaccaa tcgtcaactc ggtaacggaa tgtggcgcac gggcttccag
     2521 gaatgcatgg caggtcgtgc ccggcccgca gacccttgct ccccgcacgg cacccatggc
     2581 agaccacccg caacccggac aagcggccgg cacatccaga cattcccata aaaaacagtg
     2641 ttttacaaaa caggccgcca tcctccgaaa gcagccgcca tcgggtccgg ccaaagccgt
     2701 gacggccggc agcagtgcgc cggccaccgg tcaccgccct gtcgccccgg cccgctcccg
     2761 gcaccggatt cctccgtcac ggacggcgca cacggacgga atccatccat ggccgcaagc
     2821 cggatggcat tcgatcgcat tgcgatccgg cgcggattcc tgcaatcccc tgcctttcac
     2881 caggaccgga aaattccgca tccgcaccgt aatgcaatcc tctacctttc cggcgatgcc
     2941 ggacttccag catgcagcgc acggaatgcc ggccacagat cagcacgtcg ttttttcaaa
     3001 accttcccgc tcgcacggag aaatcgggac agaggaatga tgccgatcac acgtgaatgc
     3061 gcggttgatc cccctctccc caagcccttt cccgcgagcg ggaggagctg aaaagaccgg
     3121 acgttcagcc gggatcggcg tgatcgcacc ggcaaacgaa accggcagcc ggatggctgc
     3181 cggtcgcagg tcgaggcagg ccggacggat gctgccggcc ttttgtgccg gcagcagagc
     3241 tattgcccgg acggcggccg gtactgcgcg cgccattgcg cgtaatcggc cggcagcagg
     3301 taggtcagga tcgacttgcc attgtcctgc cggaacagat cgagcgaacg cgccggatac
     3361 agccagtgca cgccgtcctt ctcggccagc ttttcggccg gctcgccgaa acggctccgg
     3421 atcacgtcct cgtcgagccg caccagcggc gaataggtga gaccgatgat cggcagcgcg
     3481 atgatggcgt ggtcgtcggt gctcgccggg atgtactgca tcgagcccga cggcgtcgga
     3541 tcgcccttgc cggcacgcgc gatcatcgca tcgagcgcct cctgtccggc gccgagacgc
     3601 aggatcagct tggtcgtcag gccgccgagc tcgatctcgc ggtaataggc ttcgagcacg
     3661 ctgcggccgt cggcctgccg gaacaggccg agttcgacgc tgcgcccgag ccgcggcagc
     3721 acgtcctgca ggcgcgtctc gccgagccgg atgccgaaca ccgccggcac gccgtgcgcg
     3781 tcggtttcga cctgccaggg caggtcggtg ccgggcaccg acggcttgct gcccggtgcg
     3841 aacagcggca cggtcagcac gccgagcacg agtgccgccg cgccgatcca cagccccggc
     3901 cggcgcatca gccgcgttcc cgcagatcgg cggcgacgcg tttcgcgaaa tacgtcaata
     3961 cggcatcggc cccggcgcgc ttgatgcaaa gcagggattc ggcgatcacc gcttcggcga
     4021 gccagccgtt ctggatcgcc gccatctgca tcgcgtattc gccgctgacc tgatacacga
     4081 aggtcggcac gccgaattcg ctcttcacgc gccgcacgat atcgagatac ggcatgcccg
     4141 gcttgaccat gaccatgtcg gcgccctcgt cgagatcgag cgcgacttcg cgcagcgcct
     4201 cgtcgctgtt cgccgggtcc atctggtagg tgtatttgtt gcctttgccg agcgcgccgg
     4261 ccgaaccgac cgcatcgcga aacggcccgt agaaactcga tgcgtacttg gccgaatacg
     4321 cgaggatgcg cgtatggatg tggccggcgg cttcgagcgc attgcgtatt gcgccgatgc
     4381 gcccgtccat catgtcgctc ggtgccacga catcggcacc ggcctcggcg tgcgagagcg
     4441 cctgcttcac cagcacctcg acggtttcgt cgttcatcac gtagccatcg gcatcgacca
     4501 gaccgtcctg gccgtggctg gtgtacggat cgag
//
LOCUS       DAOQJH010000124         4523 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_11442_length_4523_cov_4.156893, whole genome shotgun sequence.
ACCESSION   DAOQJH010000124 DAOQJH010000000
VERSION     DAOQJH010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 4523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4523
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11442_length_4523_cov_4.156893"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1625)
                     /locus_tag="PLQ18_04085"
     CDS             complement(<1..1625)
                     /locus_tag="PLQ18_04085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE00864.1"
                     /translation="MGLFILSRRERPDPLQPAWLAAHRRHGFREPLRLTAGDWRLDLF
                     GALNGLGPQLVQRDNGDFAACTGVFFYRGARGTAGLEPLLDDFDGERFPWSGSRGHFA
                     VIVCKAGRMFLATDALGGYKVYHDGGRTLLSSSFTALRANLAQATPDAQGAWEYAWNG
                     ATYGTRTFIREIRQLRRGSLLTLGPQAEISAEYRLPAADAPQHASIDEAAEDYAARLR
                     ALFRVYAAGGGRFRTALSGGYDSRLMLALLLDAGLDPQVYVYGRAGDDDVRVAKAIAA
                     GEGLALEHVDKSQTAPPPSAGHTERAWVRFDGWKNDGLFDSGVDAEDRLLRVPGDVSV
                     LNGSAGEVFRNFFYLPDRRYRARDVVWSFYSRHDPQAMTGAFDAEAYDTAMSADLLAA
                     IGTTAPDDQALTRADIEALYPLHRARYWTARDVGLNQRFGPLLFPYLEPAVFSGTERE
                     PIAWKDYGRLEAKIIERVHPALARYPSAYGFVPADGPDLRYRLKMQLTFLRPTWLRRY
                     SYRLQQRGPLPPRPVWLGDAALSAAGLDPALPH"
     gene            complement(1728..1997)
                     /locus_tag="PLQ18_04090"
     CDS             complement(1728..1997)
                     /locus_tag="PLQ18_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018952709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator, Rof"
                     /protein_id="HNE00865.1"
                     /translation="MHIDIDDADPAADPYVPIDCELYSRYELAIMHRRTLRLVWRDEA
                     GGTHLETVLPCDLQTRMHQEFLLAETLDGEPRCLRLDRIRHVDFL"
     gene            complement(1999..2187)
                     /locus_tag="PLQ18_04095"
     CDS             complement(1999..2187)
                     /locus_tag="PLQ18_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015277958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPXCG motif-containing cysteine-rich protein"
                     /protein_id="HNE00866.1"
                     /translation="MLESWFVDCPWCGEGFEADIDTSAGDAEYVEDCRVCCAPVVFRI
                     RVAADGSLLAVETLREGD"
     gene            complement(2201..3364)
                     /gene="hemW"
                     /locus_tag="PLQ18_04100"
     CDS             complement(2201..3364)
                     /gene="hemW"
                     /locus_tag="PLQ18_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019592363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HNE00867.1"
                     /translation="MPNFTAPIPLAVYVHLPWCVRKCPYCDFNSHAATGPLPEAAYVD
                     ALLADLEHELPGVWGRRVDTVFIGGGTPSLFSPEAIERLLAGLRARLPLRPDAEITLE
                     ANPGSVEAGRYAELRAAGITRLSIGVQSFDDDQLGRLGRIHGRREAMRAAEAAHAAGY
                     PDGFNLDLMYGLEQQTPATALADLAIAIDLEPAHLSHYQLTLEPNTLFHARPPVLPDD
                     DTVADIEAACRARLEARGYERYEISAYAQPGRRCRHNLNYWEFGDYLGLGAGAHGKRT
                     DPHAGEIRRTRKARNPRDYLETAPRGTAGHEETVPEAELPFEFLLNALRLIDGVPAPL
                     YAERTGLALEALEAPLATARARGLLEADPQRLRASAHGLQFVNDLLQLFMTPR"
     gene            3566..4168
                     /locus_tag="PLQ18_04105"
     CDS             3566..4168
                     /locus_tag="PLQ18_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020505161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE00868.1"
                     /translation="MSMDKSMVLLKTVKGLDEIQNKVHGLAPKRRRVLIMVDGRMTVA
                     EMLERLSALGSDLEAQLESLLSEGFLAPAGAASARAAPAPGAAPALKPAALDASPLPG
                     QVEAVIPQFAEFNLDKAKGFARYVVLGALGPVGGKRAARIEQTRSAGELRAELDELRD
                     VLPKLMPKRQAEQVWKQLEPLMLSITEAPSVSAMPQTLPP"
     gene            complement(4165..>4523)
                     /locus_tag="PLQ18_04110"
     CDS             complement(4165..>4523)
                     /locus_tag="PLQ18_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011470117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HNE00869.1"
                     /translation="GIYSARFAGPGADDATNNARLLAELAGVPPARRSARYRCVLVYL
                     RHARDPMPLICAGTWEGTVLSAARGSGGFGYDPLFQPADCTVTAAELALADKNRLSHR
                     GRALAALHAALAAGAR"
BASE COUNT          664 a         1534 c         1692 g          633 t
ORIGIN      
        1 atgtgcggca gcgccggatc gaggccggcg gcgctgagcg cggcatcccc gagccagacc
       61 gggcgtggcg gcagcgggcc gcgctgctgc agccggtagc tgtagcggcg cagccaggtc
      121 gggcgcagga aggtcagctg catcttcagc cggtagcgga gatccgggcc gtcggccggc
      181 acgaagccgt aggcgctcgg atagcgcgcg agcgccggat gcacgcgctc gatgatcttc
      241 gcctccagcc gtccgtagtc cttccacgcg atcggctcgc gctcggtgcc gctgaacacg
      301 gccggctcca gatacgggaa cagcagcggc ccgaagcgct ggttcaggcc gacatcacgc
      361 gcggtccagt agcgcgcccg gtgcagcgga tacagcgcct cgatgtcggc gcgggtgagc
      421 gcctggtcgt cgggcgccgt cgtcccgatc gccgccagca ggtcggcgct catcgccgtg
      481 tcgtaggctt cggcatcgaa ggcgccggtc atcgcctgcg ggtcgtggcg cgagtagaac
      541 gaccagacca cgtcgcgcgc ccggtagcgc cggtcgggca ggtagaagaa gttgcggaac
      601 acctcgccgg ccgagccgtt cagcaccgag acatcgcccg gcacgcgcag caggcggtcc
      661 tcggcatcga cgccgctgtc gaacaggccg tcgttcttcc agccgtcgaa gcgcacccag
      721 gcgcgctcgg tgtggccggc cgagggcggc ggtgcggtct gcgacttgtc gacgtgttcg
      781 agcgcgaggc cttcgccggc ggcgatggcc ttggcgacgc gcacgtcgtc gtccccggcg
      841 cggccgtaga cgtagacctg cggatccagc ccggcatcga gcagcagcgc cagcatcagc
      901 cgcgagtcat agcccccgga gagcgctgtg cggaagcgcc cgccgccggc cgcatagacg
      961 cgaaacagcg cgcgcagccg cgcggcgtag tcctcggcgg cctcgtcgat cgaggcgtgc
     1021 tgcggtgcat cagcggccgg cagccggtat tcggcgctga tttccgcctg cggtccgagg
     1081 gtgagcaggc tgccgcggcg cagctggcgg atctcgcgga tgaaggtccg ggtgccgtag
     1141 gtcgcgccgt tccaggcgta ttcccaggcg ccctgggcat cgggcgtcgc ctgcgcgaga
     1201 ttcgcgcgca gggccgtgaa gctgctcgac agcagcgtgc gtccgccgtc gtggtagacc
     1261 ttgtagccgc cgagcgcgtc ggtggcgagg aacatccgcc cggccttgca gacgatcacc
     1321 gcgaaatggc cgcggctgcc ggaccacgga aagcgctcgc catcgaagtc atcgagcagg
     1381 ggttcgaggc cggccgtgcc gcgcgccccc cggtaaaaga acaccccggt gcaggcggcg
     1441 aaatcaccgt tgtcgcgctg cacgagttgc gggccgaggc cgttcagcgc cccgaacagg
     1501 tcgagccgcc agtcgccggc cgtgaggcgc agcggctcgc ggaagccgtg gcgccggtgc
     1561 gcggccagcc atgccggctg cagcggatcg gggcgctcgc gccgcgacag gatgaacaga
     1621 cccacggacg atcccccggc aggccccgga gcggggcggg gcggcactat ccgggaagcc
     1681 gccggccgcg tgcaagcgca ccgccggcgt ccgggccggc cgcggggcta caggaagtcg
     1741 acgtggcgga tgcggtccag ccgcaggcag cgcggttcgc cgtccagggt ttcggcgagc
     1801 aggaattcct gatgcatgcg cgtctgcagg tcgcagggca gcacggtttc gaggtgcgtg
     1861 ccgccggcct cgtcgcgcca gaccagccgc agcgtgcgcc ggtgcatgat cgccagctcg
     1921 tagcggctgt agagctcgca gtcgatcggc acatacgggt cggcggccgg gtcggcgtca
     1981 tcgatgtcga tgtgcatgtc agtcgccctc gcgcagggtt tcgacggcga gcagcgagcc
     2041 gtcggcagcg acgcggatgc ggaacacgac cggtgcgcag cagacccggc agtcctcgac
     2101 gtattcggca tcgccggcgg aggtgtcgat gtccgcctcg aagccttccc cgcaccacgg
     2161 acagtcgacg aaccaggatt cgagcatcgg tgcagtccct tcagcgcggg gtcatgaaca
     2221 gctgcagcag atcgttcacg aattgtaggc cgtgcgcgct ggcgcgcagg cgctgtggat
     2281 cggcttcgag caggccgcgc gcgcgcgccg tcgccagcgg cgcttcgagc gcttcgagcg
     2341 caagcccggt gcgttcggca tacagcggcg ccggcacgcc gtcgatcagg cgcagcgcgt
     2401 tcagcaggaa ctcgaacggc agctcggctt ccggcacggt ttcctcgtgg ccggccgtgc
     2461 cgcgcggggc ggtttccagg taatcgcgcg ggttgcgggc cttgcgcgtg cggcggatct
     2521 cgccggcgtg cgggtcggtg cgcttgccgt gggcaccggc accgaggccg aggtagtcgc
     2581 cgaactccca gtagttcagg ttgtgccggc agcggcggcc gggctgcgcg taggcggaga
     2641 tttcataacg ctcgtagccg cgggcttcga ggcgcgcgcg gcaggcggcc tcgatgtcgg
     2701 cgaccgtgtc gtcatcgggc agcaccgggg gccgggcgtg gaacagcgtg ttcggctcca
     2761 gcgtcagctg gtagtgcgac aggtgtgccg gctccagatc gatcgcgatc gcaagatcgg
     2821 cgagcgccgt ggccggcgtc tgctgctcca ggccgtacat cagatcgagg ttgaagccgt
     2881 cgggatagcc cgcggcgtgt gcggcctcgg cggcgcgcat cgcctcgcgc cggccgtgga
     2941 tgcgtccgag ccggccgagc tggtcgtcat cgaagctctg cacgccgatc gacagccgcg
     3001 tgatgccggc ggcgcgcagt tccgcgtagc gcccggcctc gaccgagccc ggattggctt
     3061 cgagcgtgat ctcggcatcc gggcgcagcg gcaggcgcgc gcgcaggccg gcgagcaggc
     3121 gctcgatcgc ttcgggcgag aacaggcttg gcgtgccgcc gccgatgaat acggtatcga
     3181 cacgccggcc ccagacgccc ggcagttcgt gttcgaggtc ggccagcagc gcatcgacgt
     3241 aggcggcttc cggcagcggg ccggtcgcgg cgtgcgagtt gaagtcgcag tacgggcact
     3301 tgcgcacgca ccacggcagg tgcacgtata cggcgagcgg gatcggggcg gtgaagttcg
     3361 gcatggcgta gggcccgggt gacggacgaa acggaatcac ggcgccgcat ttgttcctgg
     3421 cgccgggctt tgtcatgatt tgaaactttt cattaccaga atccggaagc cgcgcaccgg
     3481 cagcgcgcag tgaaccggac ccccagggac gccggatcgg cacctatcaa gggacatacg
     3541 gggcaagcgg aggaggactc gcggcatgag catggacaag tcgatggttt tgctgaaaac
     3601 cgtcaaggga ctcgatgaaa tccagaacaa ggtccatggc ctggcgccga aacgccggcg
     3661 cgtgctgatc atggtcgacg gccgcatgac ggtcgccgag atgctcgagc ggctctccgc
     3721 cctcggcagc gatctggaag cccagctcga aagcctgctg agcgaagggt ttctggcgcc
     3781 ggccggtgcg gcatcggcgc gcgcggcccc ggcaccgggc gctgcgccgg ccctgaagcc
     3841 ggccgccctc gatgcctccc cgctccccgg gcaggtcgag gccgtgatcc cgcagtttgc
     3901 ggagttcaac ctcgacaagg ccaagggctt cgcccgctac gtcgtgctcg gcgcgctcgg
     3961 cccggtcggc ggcaagcgtg ccgcgcgcat cgagcagacg cgctcggccg gcgagctgcg
     4021 cgccgaactc gacgagctgc gcgacgtgct gccgaagctg atgccgaagc gccaggccga
     4081 gcaggtctgg aagcagctcg aaccgctgat gctgtcgatc accgaggcgc catcggtcag
     4141 cgcgatgccg cagaccctgc cgccctaacg tgcgcctgcc gccagcgccg cgtgcagcgc
     4201 cgccagcgcg cggccgcggt ggctcagccg gttcttgtcg gcgagcgcca gttcggcggc
     4261 cgtcaccgtg cagtcggcgg gctggaacag cgggtcgtag ccgaagccgc cgctgccgcg
     4321 cgccgcgctg agcaccgtgc cttcccaggt gccggcgcag atcagcggca tcgggtcgcg
     4381 ggcgtggcgc agatacacca gcacgcagcg gtagcgcgcg ctgcgtcgtg ccgggggcac
     4441 tccggccagc tccgccagca gccgggcgtt gttggtggcg tcatcggcgc ccggcccggc
     4501 gaagcgcgcc gaatagatgc ccg
//
LOCUS       DAOQJH010000125         4520 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_11456_length_4520_cov_4.340873, whole genome shotgun sequence.
ACCESSION   DAOQJH010000125 DAOQJH010000000
VERSION     DAOQJH010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 4520)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4520)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4520
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11456_length_4520_cov_4.340873"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1673
                     /locus_tag="PLQ18_04115"
     CDS             <1..1673
                     /locus_tag="PLQ18_04115"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNE00870.1"
                     /translation="YRVVMVDDDRAFLELQSSRLQAAGFEVKALSNPLGVLEAAQGFE
                     PDVFVLDLYMPEADGCELTRLIHEEPQFANTPVMLLSGESDPTAHARVIVAGAVDVIV
                     KPADPVRFIERVRARAMQYRQNFSRARRPDRADTVTGLLTMAQFTEALEARLAHPREA
                     EGALSALLYVSLGGYLDWSKRLDAARLEQVRTRIARVISERQQGNDIVGWDNAGRVII
                     STRQPDLPNLQSYARALIDAIGGVELSDLGPGPRLVPSIGVCRADAGPVHGLIGDAAQ
                     LAAQQAREAGAGVRLQPLLVSSSAPDLEEQRRWHSIINDAIREHRLFLVYQPITNLLV
                     ADRSEWNEVLLRMRDASGQTLLPGQFLPVAAKFGLDRLLDRWVVSRALRGLDARQQDH
                     PGTVFFVKLGTASVADPSFPEWLRQMLGKVTIRPRSCVFQFSQATVLAQFERCSTLAQ
                     AVREIGQAVAIEHFDGQSDALLRVSALLPNFVKLDRSITQHLAAEPARQTQIRELVRK
                     LKEGGAAVIAAYIEDAQSLGVLWQAQVELVQGNFVQQPDEVIQHQVTS"
     gene            complement(1686..2180)
                     /locus_tag="PLQ18_04120"
     CDS             complement(1686..2180)
                     /locus_tag="PLQ18_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003194082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CreA family protein"
                     /protein_id="HNE00871.1"
                     /translation="MRVLTRLSLLCLAAVLARGAHAEEIGSVDTAFKLLGANHKIVVE
                     AFDDPKIDGIACHVAKAQTGGIKGSLGLAEDSSDASIACRQVGPVVFRESFRDGEEVF
                     TERRSILFKKLRVVRFLDRKRSTLVYLVYSDKLIDGSPKNSLSTVPVMPWPTGSVALP
                     PPAR"
     gene            2298..>4520
                     /locus_tag="PLQ18_04125"
     CDS             2298..>4520
                     /locus_tag="PLQ18_04125"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNE00872.1"
                     /translation="MGVERAARDRTAVSAGVERERIQLVALARELLLRWGQAAAQACT
                     AEALNAVSEITGRLVEECLSLDERGLAVRVRTVDTLLRELVRSGERPDSIEQVRLADA
                     IRRLHDAIVGPEAEPVPVARKAGATGPARDETGDHRPRVYVFGDAPERMAGYTTRLGE
                     HGYKAETFDDLQALHLALSLQLPQAVLLLGDAHDPRAGARLVSELRLAGLHEDLPVVL
                     VAPQTDSRCRLQALAAGADACLVEPLGGARLARRLHALIGNRQPQPHRVLLLDADPVR
                     LERLAGMLRGSGCHVRTLDDPGLLLETAAEEQPDVLVFDAGIPEADAPTLMRLIRDEP
                     ALAQLPVLLLAAEEDRLPVLTGGGVEVLPKPTAPGLLVGAVRAHAAGQRRRHGSAIRD
                     FTHRDASSGLATVEAFRKLVETALARNDAGIGALLYVAVDNLLQGDMSELRRARWRAR
                     FADALDDQLLGSDIAGMNADGAFLVFARRVDLPKLESFARDVAYALGCVTLDELGHGN
                     RPSVYVGVARAESGMLEQSVEAGKAVCVALRREGGDRVRLHPVLAGGPGGEEQRDWMG
                     RISGAIKEGRLFLMYQPITSLAGDHGERYEVLLRMRDTEGRTLLPEQFLGAATRLGLD
                     RLLDRWVVSRALRGLEGRLKEQGDTCFFIKLSGASVADPGFAEWLEAMLAKVSVQPRS
                     CVFEISEATLLVVPRRTEQLLRRVRAAGYGTALEHFAAAEESLALLPNLPLDYVKLDR
                     S"
BASE COUNT          640 a         1586 c         1618 g          676 t
ORIGIN      
        1 cgtaccgcgt ggtcatggtc gatgacgacc gcgcctttct ggagctgcag tcgagccggc
       61 tgcaggcggc cggtttcgag gtcaaggcac tgtcgaaccc gctcggtgtg ctcgaagccg
      121 cgcagggctt cgagcccgat gtgttcgtgc tcgatctcta catgcccgag gccgacggct
      181 gcgagctgac gcggctgatc cacgaggaac cgcagttcgc gaacacgccg gtgatgctgc
      241 tctccggcga gagcgatccg accgcgcatg cgcgcgtgat cgtcgccggt gccgtcgatg
      301 tcatcgtcaa gcccgccgac ccggtgcgct tcatcgagcg cgtgcgtgcc cgggcgatgc
      361 agtaccggca gaacttctcg cgcgcgcgcc ggcccgatcg cgccgacacc gtcaccggcc
      421 tgctgacgat ggcgcagttc accgaagcgc tcgaagcgcg gctcgcgcat ccgcgcgagg
      481 ccgagggcgc gctgtcggcg ctgttgtacg tgagcctcgg tggctatctc gactggtcga
      541 aacgcctcga cgcggcgcgc ctcgaacagg tgcgcacgcg catcgcgcgc gtgatcagcg
      601 agcgccagca gggcaacgac atcgtcggct gggacaacgc cgggcgcgtg atcatcagca
      661 cgcgccagcc cgatctgccg aacctgcaga gctacgcgcg ggcgctgatc gatgcgatcg
      721 gcggcgtcga gctgtccgat ctcggtccgg ggccgcggct ggtgccgagc atcggcgtct
      781 gccgcgccga cgccgggccg gtgcatggcc tgatcggcga tgccgcgcag ctcgccgcgc
      841 agcaggcgcg cgaggccggc gccggcgtgc gcctgcagcc gctgctggtg tcgagcagcg
      901 cgccggatct cgaggagcag cggcgctggc actcgatcat caacgacgcg atccgcgagc
      961 accggctgtt cctggtctac cagccgatca ccaacctgct ggtcgccgat cgcagcgagt
     1021 ggaacgaagt cctgctgcgc atgcgcgatg cttccggcca gaccctgctg ccggggcagt
     1081 tcctgccggt tgctgccaag ttcgggctcg accgcctgct cgatcgctgg gtggtcagcc
     1141 gggcgctgcg cggactcgat gcgcgccagc aggatcaccc cggcacggtg ttcttcgtca
     1201 agctcggcac ggcctcggtc gccgatccga gtttcccgga gtggctgcgc cagatgctcg
     1261 gcaaggtgac gatccggccg cgcagctgcg tgttccagtt ctcgcaggcg acggtgctcg
     1321 cgcagttcga gcgctgctcg acgctggcgc aggcggtgcg cgagatcggg caggcggtgg
     1381 cgatcgagca tttcgacggc cagtccgacg cgctgctgcg cgtctcggcg ctgctgccga
     1441 acttcgtgaa gctcgatcgt tcgatcacgc agcatctcgc cgccgagccg gcgcgccaga
     1501 cgcagatccg cgaactggtg cgcaagctca aggaaggtgg cgcggcggtg atcgccgcct
     1561 acatcgagga tgcgcagtcg ctcggcgtgc tgtggcaggc gcaggtcgaa ctggtgcagg
     1621 gcaacttcgt gcagcagccc gacgaggtga tccagcacca ggtcacgagc tgaggccgtc
     1681 gcggttcagc gcgccggcgg cggcagggcg acgctgccgg tcggccacgg catcaccggc
     1741 acggtgctca gggaattctt cggcgagccg tcgatcagct tgtcgctgta gaccagatac
     1801 accagcgtgc tgcgcttgcg gtcgagaaag cgcacgacgc gcagcttctt gaacaggatc
     1861 gaacggcgct cggtgaagac ctcctcgccg tcgcggaagc tttccctgaa caccaccggg
     1921 ccgacctggc gacaggcgat gctggcatcg gagctgtcct cggccagacc gaggctgccc
     1981 ttgatgccgc cggtctgggc cttggcgacg tggcaggcga tgccgtcgat cttcggatcg
     2041 tcgaaggctt ccacgacgat cttgtggttg gcaccgagca gcttgaaggc cgtatcgacg
     2101 ctgccgatct cctcggcgtg cgccccccgg gcaagcacgg cggcgaggca gagcagcgac
     2161 aggcgggtca ggacacgcat cggcggaact ccggtctggt gtggggtgca gggatcgtag
     2221 caaactggcc ggaacgtcca gggacggctg ccggcaaccg ttgcagccgc tgcaaacgtc
     2281 tattcaggag gtgggacatg ggagtcgagc gtgctgcacg cgatcgcacg gccgtcagcg
     2341 ccggcgtcga gcgcgaacgc atccagctcg tcgcgctggc ccgcgaactg ctgctgcgct
     2401 gggggcaggc ggcggcccag gcctgtacgg ccgaggccct gaacgccgtc agcgagatca
     2461 ccggacgcct ggtcgaggaa tgcctctcgc tcgatgaacg cggcctggcc gtgcgcgtgc
     2521 gcaccgtcga cacgctgctg cgtgaactcg tgcgcagcgg cgaacgtccg gattcgatcg
     2581 agcaggtgcg gctcgccgat gcaatccgcc gcctgcacga cgcgatcgtc ggccccgaag
     2641 ccgagccggt gccggtcgca cgcaaggccg gtgccaccgg cccggcccgc gacgagaccg
     2701 gtgaccaccg cccgcgcgtc tacgtcttcg gcgacgcgcc cgaacgcatg gccggctaca
     2761 cgacgcggct cggcgaacac ggctacaagg ccgaaacctt cgatgacctg caggccctgc
     2821 atctggcgct gtcgctgcag ctgccgcagg cggtgctgct gctcggcgac gcccatgatc
     2881 cccgcgccgg ggcccggctg gtctccgaac tgcgcctcgc cggcctgcac gaggacctgc
     2941 cggtggtgct ggtggcgccg cagaccgaca gccgctgccg gctgcaggcg ctcgccgccg
     3001 gggccgatgc ctgcctggtc gaaccgctcg gcggcgcccg gctcgcgcgc cggctgcacg
     3061 cgctgatcgg caaccgccag ccgcagccgc accgcgtgct gctgctcgat gccgatccgg
     3121 tgcgcctgga gcggctcgcc ggcatgctgc gcggcagcgg ctgtcacgtg cgcacgctcg
     3181 acgatccggg cctgctgctg gaaaccgccg ccgaggaaca gcctgacgtg ctggtgttcg
     3241 atgccggcat ccccgaggcc gacgcaccga cgctgatgcg cctgatccgc gacgagcctg
     3301 cgctcgcgca gctgccggtg ctgttgctcg ccgccgagga ggaccgcctg ccggtgctga
     3361 ccggcggcgg cgtcgaggtg ctgccgaagc cgaccgcacc gggcctgctg gtcggggcag
     3421 tgcgcgccca tgccgccggc cagcgccgcc gccacggttc ggcgatccgc gacttcacgc
     3481 accgcgacgc cagctccggg ctggcgacgg tcgaggcctt ccgcaagctg gtcgagacgg
     3541 cgctcgcgcg caacgacgcc ggcatcggcg cgctgctgta cgtggccgtc gacaacctgc
     3601 tgcagggcga catgagcgaa ctgcgccgcg cgcgctggcg tgcgcgcttc gccgatgcgc
     3661 tcgatgacca gctgctcggc agcgacatcg ccggcatgaa cgccgatggc gcctttctgg
     3721 tgttcgcgcg ccgggtcgat ctgccgaagc tcgaaagctt cgcgcgcgac gtggcctatg
     3781 cgctcggctg cgtgacgctc gatgagctcg gccacggcaa ccgcccgagc gtctatgtcg
     3841 gcgtcgcgcg cgccgaatcc ggcatgcttg aacagtcggt cgaggccggc aaggccgtct
     3901 gtgtggcgct gcgccgcgag ggcggcgatc gcgtgcgcct gcatccggtg ctggccggcg
     3961 gccccggcgg cgaggagcag cgcgactgga tggggcgcat cagcggcgcg atcaaggaag
     4021 gccggctgtt cctgatgtac cagccgatca ccagcctcgc cggtgaccac ggcgagcgct
     4081 acgaagtcct gctgcgcatg cgtgacaccg agggccgcac gctgctgccc gagcagttcc
     4141 tcggcgccgc gacccgcctc ggcctcgatc gcctgctcga tcgctgggtg gtcagccgcg
     4201 cgctgcgcgg gctcgaaggc cggctgaagg agcagggcga cacctgtttc ttcatcaagc
     4261 tctcgggcgc ctcggtcgcc gatccgggct tcgccgagtg gctggaggcg atgctggcca
     4321 aggtctcggt gcagccgcgc agctgcgtgt tcgagatctc cgaggcgacg ctgctggtcg
     4381 tgccgcggcg caccgaacaa ctgctgcgcc gcgtgcgcgc cgccggctac ggcacggcgc
     4441 tcgaacactt cgccgccgcc gaggaatcgc tggcgctgct gccgaacctg ccgctcgact
     4501 acgtcaagct cgaccgcagc
//
LOCUS       DAOQJH010000126         4504 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_11525_length_4504_cov_3.557429, whole genome shotgun sequence.
ACCESSION   DAOQJH010000126 DAOQJH010000000
VERSION     DAOQJH010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 4504)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4504)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4504
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11525_length_4504_cov_3.557429"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..364
                     /gene="dapF"
                     /locus_tag="PLQ18_04130"
     CDS             <1..364
                     /gene="dapF"
                     /locus_tag="PLQ18_04130"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013123782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HNE00873.1"
                     /translation="MGNPHAVLTVDSVAHAPVTTLGPKLEHHPRFPKRVNVGFIEGID
                     RSHVRVRVWERGAGETLACGTGACAAVVAGRLQGWLDERVEVELRGGRLTICWAGEGE
                     PVYMTGPAVRVFDGTLNF"
     gene            454..1194
                     /locus_tag="PLQ18_04135"
     CDS             454..1194
                     /locus_tag="PLQ18_04135"
                     /inference="COORDINATES: protein motif:HMM:NF016243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HNE00874.1"
                     /translation="MSTPTPLPPDPATEAGVAEWLAGHPDFFERHPELLEALSVPHLL
                     PPGVASLVERQVLLLRARETATRRKLAQLIEIARANDRLAARMHAFALGIVGLTTLED
                     LLAAITGLMRDEFGADAVALRLCAQPRDGTGGDERFVPPALWSEYAQALDGPRPACGP
                     LPPALAGRLFDAASGIASCALLPLRGCGWDGLLAIGARDAERYGAGLGTLFRQRIAEL
                     VAHTLDVFLDRCPDTGADADPDARTGDA"
     gene            1187..2119
                     /gene="xerC"
                     /locus_tag="PLQ18_04140"
     CDS             1187..2119
                     /gene="xerC"
                     /locus_tag="PLQ18_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007154303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HNE00875.1"
                     /translation="MREAVEAWLATLTHERKLSPHTLAAYRHDLDEFLGWCATRALSG
                     WPMIGHAEIRAWVATQHRQGLSGRSLQRRLAALRGLYEHLRRAGLVADNPAKLVQAPK
                     SPKKLPRALDVDQTARLLDAAPDSTLETRDRAMLELLYSSGLRLAELIALDLADLPAG
                     ADEVRVLGKGSKERIVPVGAQARAALGDWRQVRGRLAAPGEPALFVGRDGQRLGRTGV
                     ARALSRWARKRGDGTHVHPHLLRHSFASHLLESSGDLRAVQELLGHADLATTQVYTHL
                     DYQHLARVYDSAHPRARRGPGVKDGDGNGDGDGD"
     gene            complement(2352..3254)
                     /locus_tag="PLQ18_04145"
     CDS             complement(2352..3254)
                     /locus_tag="PLQ18_04145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE00876.1"
                     /translation="MNPDPESGTRLRAGLAAALLTLALPAAAADLADAITTAAAALQP
                     LAGDALAVPVCTLTLAGRAMPLLQLTERAADGKPQRRSLLLDDALALRGRLDHGSGAV
                     QRCSGDRLVIRGSVEIDGRTLDGPVLRITADAQVQADATDPNQLAIAGREPPASGRPA
                     AVVQAARAQGFDALRRAAVAPRGQTLAAWQPVCVLEAGSVRVPVLGWLALTLPKAAAG
                     DYVPPARGSSGLLLTDASGAPLAQLDVPPGAVTACHGAVLQLAQPLYGGDCKARGWRC
                     SGEEGDVVELLPGRREFGLGRSGR"
     gene            complement(3340..4113)
                     /locus_tag="PLQ18_04150"
     CDS             complement(3340..4113)
                     /locus_tag="PLQ18_04150"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNE00877.1"
                     /translation="MALTQGFKDTMTAAVADPAAADYDTALKAEIDAYVARFKGTPDF
                     PDPLDWRLFKAQLLIESGGPQGKVWKGRVMQIGNKGDPGLSVVKAAAEGRIADAEGAD
                     LVLSADLKSAMKDPKQSTDQPVLNLRIGLCYLFARLIKNIRDEDIIDTSRPPETYKVL
                     KGDSWWKIARHFRIKVKDLQDLNSSAVTRGLFPGMELHVRWGKHVRELTGWRTFDYAT
                     IADRYNGGGDADYADKLEFVHKLLLAAPKPAPTPGAPKK"
     gene            complement(4235..>4504)
                     /locus_tag="PLQ18_04155"
     CDS             complement(4235..>4504)
                     /locus_tag="PLQ18_04155"
                     /inference="COORDINATES: protein motif:HMM:NF025691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4389 domain-containing protein"
                     /protein_id="HNE00878.1"
                     /translation="QRAAYMLVFAVILAFAGSLAALLALFQLGALLLTGEPNARLRPV
                     ADGLTRYLLQLWRYQTFLTETPPFPFAEWPVSDAGAREHIVLPHG"
BASE COUNT          613 a         1666 c         1608 g          617 t
ORIGIN      
        1 gatgggcaac ccgcacgcgg tgctgaccgt cgattcggtc gcgcacgcac cggtgacgac
       61 gctcggcccg aagctcgaac atcacccgcg cttcccgaag cgcgtcaatg tcggcttcat
      121 cgaaggcatc gaccgcagcc acgtgcgcgt gcgcgtctgg gagcgcggtg ccggcgagac
      181 gctcgcctgc ggcaccggcg cctgcgcggc cgtggtcgcc ggccggctgc agggctggct
      241 cgacgagcgc gtcgaggtcg agttgcgcgg cggccggctg acgatctgct gggccggcga
      301 aggcgagccg gtatacatga ccggtccggc cgtgcgcgtg ttcgacggca cgctgaactt
      361 ctgatccgca cggacctgcc cgcatccgtg cagcgccgcg cgatccatgc ggatctgccc
      421 gatcctcccg cccccgtctc ccggagcgcc gcgatgagta caccgacacc gctgccgccc
      481 gaccccgcca ccgaagccgg cgtcgccgaa tggctggccg ggcatccgga cttcttcgag
      541 cgccatcccg aactgctcga agccctgagc gtgccgcacc tgctgccgcc cggggtcgcg
      601 tcgctggtcg aacgtcaggt gctgctgctg cgcgcgcgcg aaaccgcgac gcgccgcaag
      661 ctcgcgcagc tgatcgagat cgcccgcgcc aacgatcggc tggcggcgcg tatgcatgcg
      721 ttcgcgctcg gcatcgtcgg cctgacgacg ctcgaagacc tgctcgccgc catcaccggc
      781 ctgatgcgcg acgagttcgg cgccgacgcg gtggcgctgc ggctgtgtgc gcagccgcgc
      841 gacggcacgg gcggcgacga gcgcttcgtg ccgccggcgc tgtggagcga gtacgcgcag
      901 gccctcgacg gcccgcgccc ggcctgcggc ccgctgccgc cggcgctggc cgggcggctg
      961 ttcgacgccg cgagcggcat cgcctcctgc gcgctgctgc cgctgcgcgg ctgcggctgg
     1021 gacggcctgc tcgcgatcgg cgcccgcgac gccgagcgct acggcgccgg cctcggcacg
     1081 ctgtttcggc agcgcatcgc cgaactggtc gcgcacacgc tcgatgtctt tctcgaccgc
     1141 tgccccgaca ccggcgccga cgccgatccc gatgcgcgga ccggcgatgc gtgaggccgt
     1201 cgaagcctgg ctcgcgacgc tgacgcacga acgcaagctg tcgccgcaca cgctcgccgc
     1261 ctaccgccac gacctcgacg aattcctcgg ctggtgcgcg acgcgcgcgc tgtccggctg
     1321 gccgatgatc ggccacgccg agatccgcgc ctgggtggcc acgcagcacc ggcagggatt
     1381 gtccgggcgc agcctgcagc ggcggctggc ggcgctgcgc gggctctacg aacacctgcg
     1441 ccgcgccggc ctcgtcgccg acaacccggc gaaactcgtg caggcgccga agtcgccgaa
     1501 gaagctgccg cgcgcgctcg atgtcgacca gaccgcccgc ctgctcgacg ccgcccccga
     1561 cagcacgctc gaaacgcgcg accgcgccat gctcgaactg ctgtattcct ccggcctgcg
     1621 gctggccgag ctgatcgcac tcgatcttgc cgacctgccg gccggtgccg acgaggtgcg
     1681 agtgctcggc aagggcagca aggaacgcat cgtcccggtc ggtgcccagg cccgcgccgc
     1741 gctcggcgac tggcgtcagg tgcgcggccg gctcgccgcc cccggcgagc cggcgctgtt
     1801 cgtcggccgc gacggccagc ggctcggccg caccggcgtt gcgcgcgcac tgtcgcgctg
     1861 ggcgcgcaag cgcggtgacg gcacgcacgt gcacccgcac ctgctgcgcc acagcttcgc
     1921 gagccacctg ctcgaatcga gcggcgacct gcgcgccgtg caggaactgc tcggccacgc
     1981 cgacctcgcg accacgcagg tctacaccca cctcgactac cagcacctcg ccagggtcta
     2041 tgactcggcg catccgcgcg cccggcgcgg gccgggggtg aaggacgggg acggcaatgg
     2101 agacggggac ggggactgac gcgaaatccg cagcggcctg gcgcagtgag agggaaccga
     2161 tcggcagcgc atcgagcaga agtcggtggg catcgaacga ttgatccgcc agccgatcgc
     2221 gttcaggcat tccggatgtg ctgcgtctcg gccgtcctct gccactcgca ctgtccgctg
     2281 tcgcggctgc gcctgacgca cccagcggtg cagcgtctta gccgtgcagc cgatctgtcg
     2341 tggttcattt ctcagcgccc cgaccgtccc agcccgaact cccgccgccc cggcagcagc
     2401 tcgaccacat cgccctcttc cccgctgcag cgccagccgc gcgccttgca gtcgccgcca
     2461 tacagcggct gcgcgagctg cagcacggcg ccgtggcagg cagtgacggc gccgggcggc
     2521 acgtcgagct gcgccagagg cgcgccgctg gcgtcggtca gcagcaggcc gctgctgccg
     2581 cgcgccggcg gcacgtaatc gccggcggcg gccttcggca gtgtcagcgc cagccagccg
     2641 agcaccggca cgcgcacgct gccggcttcg agcacgcaga ccggctgcca cgccgccagc
     2701 gtctggcctc tgggcgcgac ggcggcgcgg cgcagtgcat cgaagccctg cgcgcgcgca
     2761 gcctgcacga cagccgccgg ccggccgctc gccggcggtt cgcggccggc gatcgcgagc
     2821 tggttcgggt cggtggcatc ggcctgcacc tgcgcgtcgg ccgtgatgcg cagcaccggg
     2881 ccgtcaagcg tgcggccgtc gatctcgacg ctgccgcgga tcaccagccg atcgccgctg
     2941 cagcgctgca cggcaccgct gccgtgatcg agccggccgc gcagcgcgag cgcgtcatcg
     3001 agcagcagcg agcggcgctg cggtttgcca tcggcggcgc gctccgtgag ctgcagcagc
     3061 ggcatcgccc ggccggcgag cgtcagcgtg cagaccggca cggccagcgc atcgccggcg
     3121 agcggctgca gcgcggcggc ggcggtggtg atggcatcgg cgagatcggc ggcggcggcc
     3181 ggcagcgcca gcgtcagcag cgcggcggcg aggccggcgc gcagccgggt gccggattcc
     3241 ggatcggggt tcaaggccgc tcgccgattg cggcagcggg aggcgggacc ggaagaacgg
     3301 cgctgcacat tcacctcagg cgtccgggca ggatcgattt cacttcttcg gcgcgcccgg
     3361 tgtcggcgcc ggtttcggcg ctgcgagcag cagcttgtgc acgaattcga gcttgtcggc
     3421 gtaatcggca tcgccgccgc cgttgtagcg atcggcaatc gtcgcgtagt cgaaggtgcg
     3481 ccagccggtc agctcgcgca cgtgcttgcc ccagcgcacg tgcagttcca tgcccgggaa
     3541 caggcccctg gtgacggcgc tgctgttgag gtcctgcagg tccttgacct tgatgcggaa
     3601 gtggcgggcg atcttccacc acgaatcgcc cttgagcacc ttgtaggtct cgggcgggcg
     3661 cgaggtgtcg atgatgtcct cgtcgcggat gtttttgatc aggcgcgcga acaggtagca
     3721 aagcccgatg cgcaggttca gcaccggctg gtcggtcgac tgcttcgggt ccttcatggc
     3781 cgacttgaga tcggccgaca gcacgaggtc agccccttcg gcatcggcga tgcggccctc
     3841 ggccgcagcc ttcaccaccg acaggcccgg atcgcccttg ttgccgatct gcatgacccg
     3901 gcccttccag accttgccct gcggaccgcc gctctcgatc agcagctgcg ccttgaacag
     3961 ccgccagtcg agcgggtccg ggaagtccgg cgtgcccttg aagcgggcga cgtaggcgtc
     4021 gatctcggcc ttcagtgccg tgtcgtagtc agcggcggcc gggtcggcga ccgcggccgt
     4081 catcgtgtcc ttgaagccct gcgtcagcgc catcgcggcc ccccgcgctt caacgccacc
     4141 ggagatcggt gcaatgccgg cgaagtcccg gcggaatgcg accgggccgc ggcacgcgag
     4201 gtgcgcggcg gcccggccgg aacttgcacc cgcatcagcc gtgcggcagc acgatgtgct
     4261 cgcgcgcgcc ggcgtcgctg accggccact ccgcgaacgg gaacggcggc gtctcggtca
     4321 ggaaggtctg gtagcgccag agctggagca ggtagcgcgt cagcccatcg gcgaccgggc
     4381 gcagccgcgc attcggctcg ccggtcagca gcagcgcgcc gagctggaac agcgcgagca
     4441 gcgcagcgag actgccggca aaggcgagga tcaccgcgaa caccagcatg taggcggcgc
     4501 gctg
//
LOCUS       DAOQJH010000127         4492 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_11577_length_4492_cov_3.926978, whole genome shotgun sequence.
ACCESSION   DAOQJH010000127 DAOQJH010000000
VERSION     DAOQJH010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 4492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4492
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11577_length_4492_cov_3.926978"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1443)
                     /locus_tag="PLQ18_04160"
     CDS             complement(<1..1443)
                     /locus_tag="PLQ18_04160"
                     /inference="COORDINATES: protein motif:HMM:NF020390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HNE00879.1"
                     /translation="MSRSLLCSVLLTGWLLLPPAAAAAPAPAPLSDAERTLHVLDRLA
                     YGPRPGELDAVAAEGTDAWIARQLQPDSLPLPPALLAELEALPTHGLDAAEIWRRYGP
                     PAQAALPVKERPLAEAKAKRLVLAAEQRERLLRALASPRQLEEVMTEFWFDHFNVHRE
                     KDAVMLWVGDYERSAIRPHALGRFRELLGATARHPAMLYYLDNWRSGAGGQNENYARE
                     LLELHTLGADGGYTQADVIALARILSGWTLDLAGAAEPGAPLFRFQPGRHAGGDKRLL
                     GQRIPAGGEDEGEAALDLLARHPSTARHIAYELAQRFVADQPPKPLVALLAKRFRDTD
                     GDLRAVLATLFESPQFTARAAWRAKFKTPYRYVLSAWRAGQLPIDDTAPVMTALDRLG
                     QPLWGCPTPDGYGDTAADWLGPDALRLRVDLATAWASGRLPGRDAGPDKSGRRRDAGR
                     MSPPAAGEASMAPMATMNPSTMAAAGADREA"
     gene            complement(1579..2184)
                     /locus_tag="PLQ18_04165"
     CDS             complement(1579..2184)
                     /locus_tag="PLQ18_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015280642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HNE00880.1"
                     /translation="MTAPAEIKVWDPLVRVFHWSLVVAFTLAFLTEDDWQWLHVIAGY
                     TVLGLVLVRLVWGLVGPRHARFSDFVHRPRTVWQYTQAVLRGRAERHIGHNPAGGAMI
                     VALLAMLLVTTGLGLCAYGARGNGPLGDWLGELSAFATDALTEAHELAAWLTVWLIAG
                     HLLGVLWESLLHRENLVRAMITGRKRAAKLPGGLPADPQAP"
     gene            complement(2200..2451)
                     /locus_tag="PLQ18_04170"
     CDS             complement(2200..2451)
                     /locus_tag="PLQ18_04170"
                     /inference="COORDINATES: protein motif:HMM:NF015378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HNE00881.1"
                     /translation="MRTLNTLLAAALLATAGSAFAATLTKEEAIAKAQAIHPGEVIKA
                     YTEVQHGRTLWEVQIKGEDGRKAAIFIDAETGEQDLKAK"
     gene            complement(2563..>4492)
                     /locus_tag="PLQ18_04175"
     CDS             complement(2563..>4492)
                     /locus_tag="PLQ18_04175"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNE00882.1"
                     /translation="AARTLNQHQASGIEPLHQTLLAVQIVQQHRGLSAQTLSGRTDAG
                     ATRNGKAQAITAALAALDAIVRARSDDVVLTSFWQQLPGTWQALATSVGNAQVEPAAA
                     FAAHTALIQRLLDLLGLIANHHDLALTPDKADYFLQRAIIDQLPPLAELMGQTRARGV
                     AVLTVRNDGTVTADSLTSARATGGTTRSSISEADRLEMQRLHDAIQHQARSVSGTLTA
                     ITELDPALGERLRTPVTAANAQVEELAQLVLWEFIEADTIRFDAGEFFRISTAAIDVQ
                     FALIDSLLDALETRIEVQAGALQRQQQLLAAAMVGIALLTGGLTLAIVRTITAPLSQT
                     ARVLQTAAGGDLTRRLRPRSRDELGHMGRDLDVFLDALSRSIGEIADTAQQLGQSSQA
                     LTEVSRRVNDGAARTTTDAEKLSIAAADMNERVASVATATSQMNTAVHEIARSTAEGA
                     RVTGEAVSLASAATTAMQRLDESSARIGEVVALISHITRQTRLLALNATIEAARAGEA
                     GRGFGVVAHEVKELADGTARATQDIADKIGTIQSDTAGAVEAIRGIGDSVGRVNTLQT
                     TIAAAIEEQSVVTDDISRNIAQAATRTGEIGSGLIEVTQIAAGAAASGRELESAAHAL
                     AQMGAQLRDLVQRFRYAT"
BASE COUNT          589 a         1526 c         1683 g          694 t
ORIGIN      
        1 ggcttcgcgg tctgcgccgg ctgcggccat cgtcgacgga ttcatcgtgg ccatgggggc
       61 catcgatgcc tcgccggcgg ccggcggcga catcctgccg gcgtcgcgcc ggcgcccgga
      121 cttgtcgggg ccggcatcgc gccccggcag ccggccgctc gcccaggccg tggccagatc
      181 gacgcgcagg cgcagcgcat cggggccgag ccagtcggcg gcggtgtcgc cgtagccgtc
      241 cggcgtcggg cagccccaca gcggctggcc gaggcgatcg agcgcggtca tgaccggcgc
      301 ggtgtcgtcg atcggcagct gcccggcgcg ccaggccgac agcacgtagc ggtacggcgt
      361 cttgaacttc gcgcgccagg ccgcgcgcgc cgtgaactgc gggctttcga acagcgttgc
      421 cagcacggcg cggaggtcgc cgtcggtgtc gcggaagcgc ttggcgagca gcgcgaccag
      481 cggcttcggc ggctgatcgg cgacgaagcg ctgcgcaagc tcataggcaa tgtgccgtgc
      541 cgtcgacgga tggcgcgcga gcaggtcgag ggcggcctcg ccttcgtcct cgccgccggc
      601 cgggatgcgc tggccgagca gccgcttgtc gccgcccgca tgacggccgg gctggaagcg
      661 gaacagcggc gcgccgggct cggcggcccc ggcgagatcg agcgtccagc cgctcaggat
      721 gcgcgcgagc gcgatgacat cggcctgcgt gtagccgccg tcggcgccga gcgtgtgcag
      781 ctccagcagt tcgcgcgcgt agttctcgtt ctggccgccg gcgccgctgc gccagttgtc
      841 gaggtaatac agcatcgccg gatggcgggc ggtggcgccg agcagctcgc ggaagcgccc
      901 gagcgcgtgc gggcggatgg cgctgcgctc gtagtcgccg acccacagca tcacggcatc
      961 tttctcgcga tggacgttga agtgatcgaa ccagaactcg gtcatcacct cttcgagctg
     1021 gcgcgggctt gcgagcgccc gcagcaggcg ttcgcgctgc tcggcggcga gcacgaggcg
     1081 cttggccttc gcctcggcga gcgggcgctc cttcaccggc agtgccgcct gcgccggcgg
     1141 gccgtagcgg cgccagattt cggcggcatc gaggccgtgc gtcggcagcg cttcgagttc
     1201 ggcgagcagc gccggcggca gcggcagcga atcgggctgg agctggcggg cgatccaggc
     1261 gtcggtgcct tcggcagcga cggcatcgag ctcgccgggc cgcggcccgt aggcgagccg
     1321 gtcgagcacg tgcagcgtgc gttcggcgtc ggaaagcgga gcgggagcgg gggcggcagc
     1381 ggcggccggg ggcagcagca gccagccggt gagcagcacg gagcacagca gggatcgtga
     1441 catctgcgcc acctgacgca cgccggtgac ccggcgtgcc tgaggaatca ggggcttgag
     1501 cttactggag cgtggcggtc gcgggtatcc ggcatcggcc cggggcgaca ggatttgcgt
     1561 ttcgtgaccg atcggcgctc atggcgcctg cgggtccgcc ggcagcccgc ccggaagctt
     1621 tgcggcgcgc ttgcggccgg tgatcatcgc gcgcaccagg ttctcgcggt gcagcaggct
     1681 ctcccagagc acgccgagca gatggccggc gatcagccac accgtcagcc aggcggccag
     1741 ctcgtgcgct tcggtcagcg catcggtcgc gaaggcgctc agctcgccga gccagtcgcc
     1801 gagcggtccg ttgccgcgcg caccgtacgc gcagaggccg agtccggtcg tcaccagcag
     1861 catcgcgagc agcgcgacga tcatcgcgcc gccggccgga ttgtggccta tgtggcgctc
     1921 ggcgcgaccg cgcagcaccg cctgcgtgta ctgccagacg gtgcgcggcc ggtgcacgaa
     1981 gtcgctgaag cgcgcatgcc gcggaccgac cagcccccag accaggcgca cgagcaccag
     2041 cccgagcacc gtgtagccgg cgatgacgtg cagccactgc cagtcgtcct cggtgaggaa
     2101 ggccagcgtg aacgccacga ccagcgacca gtggaagacc cgcaccagcg ggtcccagac
     2161 cttgatttca gcgggggcgg tcatgtcggc agcccgcgct tacttggcct tcaggtcctg
     2221 ctcgccggtc tcggcgtcga tgaagatcgc cgccttcctg ccgtcttcgc ccttgatctg
     2281 cacttcccac agcgtgcggc cgtgctggac ttcggtatag gccttgatga cttcgccggg
     2341 gtggatggcc tgggccttgg cgatcgcctc ctccttggtg agggtggcgg cgaaggcgct
     2401 gccggcggtg gcgagcaggg cggcggcgag cagggtattg agagtgcgca tgatcggaga
     2461 actccgcagg gtgggaatga ccatgcgaag tatatggaca cctgcgtcgg gccagactta
     2521 gcaaaccgtt atgtacctat gcgccggatg atgactatgc gttcaggttg cgtagcggaa
     2581 gcgctgcacg agatcgcgca gctgcgcgcc catctgcgcc agtgcgtgcg cggcgctttc
     2641 cagctcccgg ccggaggccg cggcgccggc ggcgatctgc gtgacctcga tgaggccgct
     2701 gccgatctcg ccggtgcgcg tggccgcctg cgcgatgttg cggctgatgt cgtcggtgac
     2761 cactgactgt tcctcgatcg ccgcggcgat cgtggtctgc agcgtgttca cgcgcccgac
     2821 gctgtcgccg atgccgcgga tcgcctcgac ggcgccggcg gtgtcggact ggatcgtgcc
     2881 gatcttgtcg gcgatgtcct gcgtggcgcg ggccgtgccg tcggcgagtt ccttgacctc
     2941 gtgcgcgacc acgccgaagc cgcgtccggc ctcgccggcg cgcgccgcct cgatggtcgc
     3001 gttcagcgcc agcagccgcg tctggcgcgt gatgtggctg atcagcgcca cgacctcgcc
     3061 gatgcgcgcg ctgctctcat cgaggcgctg catcgcggtc gtcgccgcgc tggcgagcga
     3121 caccgcctcg ccggtgacgc gcgcgccctc ggcggtgctg cgtgcgatct cgtgcacggc
     3181 ggtgttcatc tggctggtgg cggtcgcgac gctggcgacg cgctcgttca tgtcggcggc
     3241 cgcgatgctg agcttctcgg catcggtggt cgtgcgcgcc gccccgtcgt tgacgcggcg
     3301 gctgacctcg gtcagcgcct gcgacgactg cccgagctgc tgcgcggtgt cggcgatctc
     3361 gccgatgctg cgcgacagcg catcgaggaa cacatcgagg tcacgcccca tgtgtccgag
     3421 ctcgtcgcgc gagcgcgggc gcagccgccg ggtcagatcg ccgccggcgg cggtctgcag
     3481 cacgcgcgcc gtctgcgaca gcggtgcggt gatcgtgcgc acgatcgcca gcgtcagccc
     3541 gccggtcagc agcgcgatcc cgaccatggc tgcggcgagc agctgctgct ggcgctgcaa
     3601 cgcgcccgcc tgcacttcga tgcgggtttc gagcgcatcg aggagcgagt cgatcagggc
     3661 gaactggaca tcgatcgccg ccgtgctgat gcggaagaac tcgccggcat cgaagcggat
     3721 ggtgtcggct tcgatgaatt cccagagcac cagctgcgcc agttcctcga cctgcgcatt
     3781 cgccgccgtg accggcgtgc gcagccgttc gccgagcgcc gggtccagtt cggtgatggc
     3841 ggtgagcgta ccgctgacgc tgcgtgcctg atgctggatc gcgtcgtgca gccgttgcat
     3901 ctccagccgg tcggcctcgc tgatgctgct gcgggtcgta ccgccggtgg cccgtgccga
     3961 cgtgagcgaa tcggccgtga ccgtaccgtc gttgcggacc gtgaggactg ccacgccgcg
     4021 cgcgcgcgtc tggcccatca gctcggcgag cggcggcagc tggtcgatga tcgcgcgctg
     4081 caggaagtaa tcggccttgt ccggagtcag cgccagatcg tggtgattgg cgatcagccc
     4141 cagcagatcg agcagccgct ggatcagggc ggtatgggcg gcaaaggctg cggccggctc
     4201 gacctgggcg ttgccgaccg aggtggccag cgcctgccag gtgcccggga gctgctgcca
     4261 gaacgaagtg agcacgacat cgtcgctgcg cgcccgcacg atggcatcga gcgcggccag
     4321 cgccgcggtg atggcctgcg ccttgccgtt gcgggtggcg ccggcatcgg tgcgtccgga
     4381 cagcgtctgc gccgaaaggc cgcggtgctg ctgcacgatc tgcacggcca gcagcgtctg
     4441 gtgcaagggc tcgatgccgc tcgcctgatg ctggttgagc gtgcgggccg cg
//
LOCUS       DAOQJH010000128         4492 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_11578_length_4492_cov_3.678837, whole genome shotgun sequence.
ACCESSION   DAOQJH010000128 DAOQJH010000000
VERSION     DAOQJH010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 4492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4492
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11578_length_4492_cov_3.678837"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..622
                     /locus_tag="PLQ18_04180"
     CDS             <1..622
                     /locus_tag="PLQ18_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017939847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNE00883.1"
                     /translation="PQPPQHHPEIDTGVHVLLVLDQAVRLGADVPTRYAALLHDLGKG
                     TTPPDEWPKHTAHEARGAALAREVSERLRAPKEFRELAVLVAELHTRAHRALELRTTT
                     LLETLERLDALRRPERFARWLLACEADARGRTGLETRPYPQAAWLAQAREAAAAVPVQ
                     PLVERGLKGPEFGEALRLARLDALRAARKRRAAAADAAGEGSAATS"
     gene            702..920
                     /locus_tag="PLQ18_04185"
     CDS             702..920
                     /locus_tag="PLQ18_04185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE00884.1"
                     /translation="MNVGNIGSSSIEQFISNQRPPSQSEGAPQQRHVEAERQQASEAL
                     VRSAPAAPKDIPAFDPERRIGAYIDVTA"
     gene            1297..2841
                     /locus_tag="PLQ18_04190"
     CDS             1297..2841
                     /locus_tag="PLQ18_04190"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="PEP-CTERM proteins occur, often in large numbers,
                     in the proteomes of bacteria that also encode an
                     exosortase, a predicted intramembrane cysteine proteinase.
                     The presence of a PEP-CTERM domain at a protein's
                     C-terminus predicts cleavage within the sorting domain,
                     followed by covalent anchoring to some some component of
                     the (usually Gram-negative) cell surface. Many PEP-CTERM
                     proteins exhibit an unusual sequence composition that
                     includes large numbers of potential glycosylation sites.
                     Expression of one such protein has been shown restore the
                     ability of a bacterium to form floc, a type of biofilm;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HNE00885.1"
                     /translation="MRTLACSALVLVTGLMPVAVVHAAPFPAGPLGSSSQSTYLLPSM
                     TGVALTPLLTVGDAAGNGYRMAGIPDGLGAFDNGDGSFTLLMNHELGASAGAVHAHGA
                     TGAFVSEWVIDKSTLTVLSGRDQIQTVNLLNTSTGQYQPAINATLTRLCSADLPAPTA
                     FFNPGSGLGTSTRFFMNGEETSGGRAFVHTVTGADAGQSYEFAALGHAAWENVVARPT
                     ADDRTVVLGTDDTHPNGDAANAKGRVYVYFGDKQSTGTDLEKAGLTNGTTWAIKVNGA
                     AIEDRSATASFGLDADGKASFSLVDDGSGTNFLRPEDITWDTQNPDRAYFVTTDGLDD
                     ADPASRTSRLWRITLNDVNDPAAGGVIELLIDGRQPGQPQMMDNLTVAADGTVLIQED
                     VGNTARLGKVWRYDPNTKTTVEVAAHDPARFLLGAPGFLTQDEESSGIIDVTALFDGV
                     AGYDTGTYQYFLLDVQAHYALTDPALVEGGQLLLMAVQAVPEPGSLSLMAAMLGFTLY
                     GARRKR"
     gene            3173..>4492
                     /locus_tag="PLQ18_04195"
     CDS             3173..>4492
                     /locus_tag="PLQ18_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011745732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Eco57I restriction-modification methylase
                     domain-containing protein"
                     /protein_id="HNE00886.1"
                     /translation="MARLAAEDDAEARGAVFTRIEVVEFILDLVGYTPDRPLHACRLL
                     EPAAGCGEFLRVAVRRLLAAWRAAGSPGDTDILAVLGPALQAVELHHDSCRRARAALG
                     EELAAAGIGADTAQALIRQWLRQGDFLLSALPASFDFVVGNPPYVRQELIPPVLLAEY
                     RRRYATLYDRADLYVPFIERALSLLAAGGRLGFICPDRWMKNRYGGPLRRLVAEGFHL
                     QAVIDMDETPAFHSEVAAYPAIVVIARARPGRTRIAHRPPLDAGVLGPLAAALQAAAK
                     PDPSLPEVREQQLVCLRGSDAPWLLDTDARTNLIRRIEAGFPLLEATGCRVGIGVATG
                     ADRVFIGDYASLDVEPQRKLPLAMTADLESGELRWRGLGVVNPFGDDGRLVDLRDWPR
                     LRAYLERHRDVIAARHCARKSPGNWYRTIDRIHPALAAEPKLLIPDIK"
BASE COUNT          667 a         1679 c         1490 g          656 t
ORIGIN      
        1 gccgcaaccg ccgcagcatc acccggagat cgacaccggc gtgcacgtgc tgctggtgct
       61 cgaccaggcc gtgcgcctcg gcgccgacgt gccgacgcgc tacgcggcgc tgctgcacga
      121 cctcggcaag ggcacgacgc cgccggacga atggccgaag cacactgcgc acgaggcacg
      181 cggcgcagca ctcgcccgcg aggtcagcga gcgcctgcgc gcgccaaagg aattccgcga
      241 actcgctgtg ctggtggccg agctgcacac gcgcgcgcac cgtgcgctgg agctgcgcac
      301 gacgacgctg ctggaaaccc tcgaacgcct cgacgcgctg cgccgccccg agcgcttcgc
      361 gcgctggctg ctcgcctgcg aggccgatgc gcgcgggcgc accgggctgg aaacgcggcc
      421 gtatccgcag gctgcatggc tcgcgcaggc gcgcgaggcg gcagcggcgg tgccggtgca
      481 gccgctggtc gagcgcggcc tcaagggtcc ggagttcggc gaggcgctgc gtctggcacg
      541 cctcgatgcg ctgcgcgcgg cgcgcaagcg ccgggccgca gcggcggacg cggcggggga
      601 ggggagtgcg gcgacatcgt gagaagccgt tgcgacgact ggcatttcca ttacatcagc
      661 gtaaggttgc tgcctgccgt acccgtcaag gagtcaccgc catgaacgtc ggcaacatcg
      721 gctcgtcctc gatcgagcag ttcatcagca accagcgtcc gccctcccag tccgaaggcg
      781 ccccgcagca gcgccacgtc gaagccgagc gccagcaggc cagcgaagcc ctggtccgct
      841 ccgcccccgc cgccccgaag gacatccccg ccttcgaccc ggaacggcgc atcggcgcct
      901 atatcgacgt cactgcctga aaccaccgcg cttacgcccc cacgccccga agccatgccg
      961 gttccggggc cgggtggcag tgccgctcac ctttgctcaa ggtgcagcaa acccgtcgca
     1021 agccgcagca tcccggccgc catcactgcc gatcccgctg ccacgttcca cgtgaaaccc
     1081 cgcccggcgc tccacgggcg ctgtctttga actgccgcag ggctttcaca caggccgcgt
     1141 agcgtccgcc tgcccgcgga ccgcacgcgg cgcggtcgct gccgggttca gggaacgcag
     1201 agtccgtccg gcagcttccg gtgccgtagc cgctgcgtcc gcaggcgtcg atccctcgac
     1261 cgcttccctt ccgtagcctg cacggagcct gttcccatgc gcacgcttgc ctgttccgcc
     1321 ctggtgctcg tcaccggcct gatgcctgtt gccgtcgtcc acgccgcgcc gttcccggcc
     1381 ggtccgctcg gcagctcgtc ccaatccacc tatttgctgc cgtcgatgac cggcgtcgcg
     1441 ctgaccccgc tgctgacggt cggcgatgcc gccggcaacg gttaccggat ggccggcatc
     1501 cccgacggcc tcggggcctt cgataacggc gacggcagct tcacgctgct gatgaaccac
     1561 gaactcggcg ccagcgccgg tgccgtgcat gcgcatggcg ccaccggcgc cttcgtctcc
     1621 gagtgggtca tcgacaagag cacgctgacg gtgctgagcg gtcgcgacca gatccagacc
     1681 gtcaacctgc tgaacacctc caccggccag taccagccgg cgatcaacgc cacgctgacg
     1741 cgcctctgct cggccgacct gccggcgccg accgccttct tcaacccggg cagcggtctg
     1801 ggcaccagca cgcgcttctt catgaacggc gaggagacca gcggcggccg ggcgttcgtg
     1861 cacaccgtca ccggcgccga tgccggccag agctacgagt tcgccgcgct cggccacgcc
     1921 gcctgggaga acgtcgtcgc ccggccgact gccgatgacc gcaccgtcgt gctcggcacc
     1981 gacgacaccc atccgaacgg cgatgccgcc aacgccaagg gccgcgtcta cgtctacttc
     2041 ggcgacaagc aatcgaccgg caccgatctg gagaaggccg gcctgaccaa cggcacgacc
     2101 tgggcgatca aggtcaacgg cgcggcgatc gaggatcgtt cggccacggc cagcttcggg
     2161 ctggacgccg acggcaaggc cagcttctcg ctggtcgatg acggcagcgg caccaacttc
     2221 ctgcgccccg aggacatcac ctgggatacg cagaacccgg accgcgccta cttcgtgacc
     2281 accgacggtc tcgacgacgc cgacccggcc tcgcgcacca gccggctgtg gcggatcacg
     2341 ctcaatgacg tgaacgaccc ggccgccggt ggcgtcatcg aactgctgat cgacggccgc
     2401 cagcccggcc agccgcagat gatggacaac ctgacggtcg ccgccgatgg cacggtgctg
     2461 atccaggaag acgtcggcaa cactgcgcgt ctgggcaagg tctggcgcta cgacccgaac
     2521 acgaagacca cggtcgaggt cgccgcccac gacccggcgc gcttcctgct cggcgccccc
     2581 ggcttcctga cccaggacga ggaatcctcc ggcatcatcg acgtgaccgc gctgttcgac
     2641 ggcgtcgccg gctacgacac cggcacctac cagtacttcc tgctcgacgt gcaggcgcac
     2701 tacgcgctca ccgacccggc gctggtcgag ggcgggcagt tgctgctgat ggcggtgcag
     2761 gcggtgccgg aaccgggcag cctgtcgctg atggcggcga tgctcggctt cacgctctac
     2821 ggcgcccgcc gcaagcgctg acgcccgccc cggcggcaga gcgggccgcc gcggaatctg
     2881 tctgaacctg cctgaacctg cctgaacgaa cggcgcaccg gaccggcggt gcgccgtttt
     2941 tcattgtgct ggcacgggtg gaagagggcc gcgcgcagcg gatagtcgcc catgcggcag
     3001 gcagtaccgg cttccacgcc gcacatgcgc agccggagca gccccagtac gatgttccgg
     3061 cgcgatgatc tcgccgctgc cgtggaatcg tctcttgtcc tcgctgcccc tgtttcccga
     3121 taccgacgcg ctcacggccc agccggtctg tgcgcccgcc gtcaccgccg ccgtggccag
     3181 actcgcggcc gaggacgatg ccgaagcccg cggtgcggtg ttcacgcgca tcgaggtcgt
     3241 cgagttcatt ctggatctgg tcgggtatac gccggaccgg ccgctgcatg cctgccgtct
     3301 gctggaaccg gccgccggtt gcggcgaatt cctgcgggtg gccgtgcgcc gcctgctcgc
     3361 ggcctggcga gcggctggta gccccggcga caccgacatc ctggccgtgc ttggtccggc
     3421 gctgcaggcc gttgagctgc atcacgacag ctgccgtcgt gcccgcgccg cactcggcga
     3481 ggaactggcc gcggccggca tcggggcgga cacggcgcaa gccctgatcc ggcaatggct
     3541 gcggcagggc gacttcctgc tgagcgcgct gccggcctcg ttcgacttcg tcgtcggcaa
     3601 tccgccctac gtgcggcagg agctgattcc gccggtgctg ctggccgaat accgccgccg
     3661 ttacgcgacg ctctacgatc gcgccgacct gtacgtgccg ttcatcgaac gcgcgctgtc
     3721 gctgctcgcc gccggtggcc ggctcggctt catctgcccg gaccgctgga tgaagaaccg
     3781 ctacggcggc ccgctgcgcc ggctggtggc cgaaggcttc cacctgcagg cggtcatcga
     3841 catggatgaa acgcccgcct tccacagcga ggtggccgct tatccggcca tcgtcgtgat
     3901 tgcccgcgcc cggcccggcc gcacgcgcat cgcccatcgt ccgccgctcg atgccggagt
     3961 gctcgggccg ctggctgccg cgctgcaggc cgcggcaaag cctgatccgt cgctgccgga
     4021 ggtgcgcgag caacaactgg tctgcctgcg cggcagtgat gcaccgtggc tgctggatac
     4081 cgatgcgcgc acgaacctga tccgccgcat cgaagccggt ttcccgctgc tcgaagccac
     4141 cggctgccgg gttggcatcg gggtcgcgac cggagccgat cgcgtgttca tcggcgacta
     4201 cgccagcctc gatgtcgagc cgcaacgcaa gctgccgctg gcgatgacgg ccgacctcga
     4261 atccggtgag ctgcgctggc gcgggctcgg ggtcgtcaat cccttcggcg acgatggccg
     4321 gctggtcgat ctgcgcgact ggccacggct gcgcgcgtat ctggagcggc accgggacgt
     4381 gatcgcagcc cgccactgcg cgcgcaagtc tcccggcaac tggtaccgga ccatcgaccg
     4441 catccacccg gcgctggccg cggaaccgaa gctgctgatc ccggacatca ag
//
LOCUS       DAOQJH010000129         4490 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_11590_length_4490_cov_3.645772, whole genome shotgun sequence.
ACCESSION   DAOQJH010000129 DAOQJH010000000
VERSION     DAOQJH010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 4490)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4490)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4490
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11590_length_4490_cov_3.645772"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(40..402)
                     /locus_tag="PLQ18_04200"
     CDS             complement(40..402)
                     /locus_tag="PLQ18_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012334507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HNE00887.1"
                     /translation="MSFACTLPPRPLLAAALPVLLALLVLSPARAAPAAVTVTIHGYA
                     FEPRNLVVAPGTRILWKNADDVPHNVVGLGSAAALRSPALDQDDSWETQLDAPGEIDY
                     VCGLHPHMQGHISVRAAQ"
     gene            complement(499..1497)
                     /locus_tag="PLQ18_04205"
     CDS             complement(499..1497)
                     /locus_tag="PLQ18_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015927519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNE00888.1"
                     /translation="MSSSRSDRSASPAAPSIASGQPPSAGLTRRQALGCLFWAGTGAL
                     WAMHGGVPRWLGAIGDANAAEAKTDAGAFRFVQISDSHIGFNKAANPDPLLTLNLALD
                     KVRALPERPALMLHTGDVTHLSKPAEFDTAAQLIGGLGLPTFYVPGEHDVIDEGAGRA
                     WLDRYGKGTQGAGWYSFEDHGVHFVGLVNVLDFKPGGLGRLGAAQLAWLADDLKAVRD
                     STPVVVFAHIPLWTVYADWGWGTDDAAQALALLKRFGSVTVLNGHIHQTLQKVEGNVS
                     FHTAYSTAYPQPAPGTAASPGPLVVPAEQLRHVLGVRSLQLTRAAPGAQLTDYPLA"
     gene            complement(1568..2290)
                     /locus_tag="PLQ18_04210"
     CDS             complement(1568..2290)
                     /locus_tag="PLQ18_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010321751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HNE00889.1"
                     /translation="MPVTLPAAERTAGATLHEQVYQSLRGAIMCGRFVPGRALTIRGV
                     AAMLGTSLMPVREALRRLVAERALELLANRRVSVPHMTPARLDELCAARLALEPLAAV
                     RALPHVDGTRLAELRQIDAGIDAALAAGEVERYLQLNQLFHLTLYRAGEPQVLIPLIE
                     SLWLQFGPFMRATIGRIGASYVLDRHVEALAALERRDPLALRLAIESDIRDGMGSLSH
                     EELLRLYRDAAAPAERSASARE"
     gene            2408..3784
                     /locus_tag="PLQ18_04215"
     CDS             2408..3784
                     /locus_tag="PLQ18_04215"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013331658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HNE00890.1"
                     /translation="MSPANVAHDEVGRFLAAHPEVEVFELLIPDTNGVLRGKWVPRSA
                     VEKVYSAGIRLPSSVFALDIWGNDVLASGLVIETGDRDGLCKPVAGSLTLVPWLKRPT
                     AQVLMAMYDPDSVQPFFGDPRQVLAQVLAGYTALGLTPVVALELEFYLLDREPSPDGS
                     PQPPLSPGTGRRQWSSQVYGIDELHEFEDFFDGVARACVLQRLPVDTTVAEQAPNQYE
                     INLKHVDDAILAADNAVLLKRVIKGVAQQHQMAATFMAKPYGELAGNGMHIHFSLIDE
                     RGFNVFDDGSAEGSALLKHAIAGMVTTMRETMALYAPNTNSYRRFQPGSHAPVSANWG
                     YDNRAAALRIPSGERQAMRIEHRLAGADANPYLALAAMLAGGLRGIREKLDPGEPSRG
                     HVGNGQAPVLPASLKHALEIFDKSPFPREAFGDSYPRLYSACRWQELEKFGKQVTPLE
                     YDSYLRTV"
     gene            3846..4301
                     /locus_tag="PLQ18_04220"
     CDS             3846..4301
                     /locus_tag="PLQ18_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006231892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE00891.1"
                     /translation="MTRLSSEDAEMAHEKKIAHLTFIQGIITRMGNNSFLLKGWTVTL
                     VAALFALAAKDSNQNFILIAYFPILVFWMLDGYFLYQERLFRDLYNGVANDRISSEQF
                     EINPASLMSDAGKWQPAFLWARAALSKTLLVFYMAMMGVTLFVMCFLLR"
BASE COUNT          703 a         1470 c         1595 g          722 t
ORIGIN      
        1 aagccgccat gcccggccgc tgccggccgg gcagcgggtt cattgcgcag cgcgcacgct
       61 gatgtggccc tgcatgtgcg gatgcaggcc gcagacgtag tcgatctcgc ccggcgcatc
      121 gagctgtgtc tcccagctgt cgtcctgatc gagtgccggc gagcgcagcg cggccgcgct
      181 gccgaggccg acgacgttgt gcggtacgtc atcggcgttc ttccagagga tgcgcgtgcc
      241 gggcgcgacg acgaggttgc gcggttcgaa agcgtatccg tggatcgtga ccgtgaccgc
      301 tgcgggggcg gcgcgggccg gggacagcac gagcagggcc agcagcaccg gcagggcggc
      361 ggcaaggagc gggcggggcg ggagggtgca ggcgaaggac atggcaggga ttccggaggt
      421 cgggcggcga tggacgcggg gccgggcggc tcgaaagcgc cggtgctcgg cgtcggggca
      481 gaaggcgcgg gccggccgtc aggccagcgg gtagtcggtc agctgcgcac cgggtgcggc
      541 gcgggtcaac tgcaggctgc gcacgccgag cacgtgccgg agctgttcgg ctggcaccac
      601 cagcgggccg ggcgaggccg cggtgccggg ggccggctgc gggtaggcgg tcgagtaggc
      661 ggtgtggaag ctgacgttgc cctcgacctt ctgcagcgtc tggtggatgt ggccgttcag
      721 caccgtcacc gagccgaagc gcttcagcag cgcgagcgcc tgcgcggcgt cgtcggtgcc
      781 ccagccccag tcggcgtaca cggtccacag cgggatgtgt gcgaacacca cgaccggcgt
      841 cgaatcgcgc acggccttca ggtcatcggc gagccacgca agctgcgcgg ccccgagccg
      901 gccgaggccg ccgggcttga agtcgagcac gttgaccagg ccgacgaaat gcacgccgtg
      961 gtcctcgaag ctgtaccagc cggcgccctg cgtgcccttg ccgtagcgat cgagccaggc
     1021 ccgcccggcg ccttcgtcga tcacgtcgtg ctcgcccggc acgtagaagg tcggcagccc
     1081 gagtccgccg atcaactgcg cggcggtgtc gaactcggcc ggcttcgaca ggtgcgtgac
     1141 atcgccggtg tgcagcatca gcgccggacg ctcgggcagc gcgcgcacct tgtcgagcgc
     1201 gaggttcagc gtcagcagcg gatcggggtt cgcggccttg ttgaagccga tgtggctgtc
     1261 gctgatctgc acgaagcgga aggcaccggc atcggtcttg gcctcggccg cattcgcgtc
     1321 accgatcgca ccgagccagc gcggcacgcc gccgtgcatt gcccacagcg cgccggtgcc
     1381 ggcccagaac aggcagccga gcgcctgccg gcgcgtgagg ccggcggatg gcggctgtcc
     1441 ggatgcgatg gagggggcgg cgggtgaagc gctgcggtcg gaacgggatg aggacatggg
     1501 ggcggtctcc gctgctgcgg ccccggaagc gggaccgttg cacgggagac gcgccgggcc
     1561 gggtggttta ttcccgggcg gacgcagatc gctctgccgg tgccgcagcg tcccggtaca
     1621 gccgcagcag ttcctcgtgg ctcagcgagc ccatgccgtc gcggatgtcg ctctcgatcg
     1681 ccaggcgcag cgccagcgga tcgcgccgtt cgagcgcggc cagcgcctcg acgtggcgat
     1741 cgagcacgta gctcgcgccg atgcggccga tggtcgcgcg catgaacggc ccgaactgca
     1801 gccacaggct ctcgatcagc gggatcagca cctgcggctc gccggcgcgg tacagcgtca
     1861 ggtgaaagag ctggttgagc tgcaggtaac gctcgacctc gccggcagcg agcgcggcgt
     1921 cgatgccggc gtcgatctgc cggagttccg cgagccgcgt gccgtcgacg tgcggcagcg
     1981 cccgcaccgc cgccagcggc tccagcgcga ggcgcgcggc gcagagctca tcgagccgcg
     2041 ccggcgtcat gtgcggcacg ctgacgcggc ggttggcgag cagttccagc gcgcgctcgg
     2101 cgaccagccg gcgcagcgcc tcgcgcaccg gcatcaggct ggtgccgagc atcgccgcga
     2161 cgccgcggat ggtcagcgcc cgccccggca cgaagcgccc gcacatgatc gcgccgcgca
     2221 gcgactggta gacctgttcg tgcagggtgg cgccggcggt gcgctcggcg gcgggcagcg
     2281 tcacgggcat cggggtggga ggcggtgggc tcgacagctc cggaaacatg tgatcacaat
     2341 cggccggcag tcttcgcgaa cacccgaacg gcgtcaagcg atgcccctgc ccccgaggaa
     2401 tcctccgatg agtcccgcca acgtcgcgca cgacgaggtc ggccgcttcc tggccgcgca
     2461 tcccgaagtc gaagtcttcg agctgctgat cccggacacc aacggcgtgc tgcgcggcaa
     2521 gtgggtgccg cgcagtgcgg tcgagaaggt ctacagcgcc ggcatccgcc tgccttcgtc
     2581 ggtgttcgcg ctcgacatct ggggcaacga cgtgctcgcc agcggcctcg tgatcgagac
     2641 cggcgaccgc gacggcctct gcaagccggt cgccggttcg ctgacgctgg tgccgtggct
     2701 caagcgcccg accgcgcagg tgctgatggc gatgtacgac ccggacagcg tccagccctt
     2761 cttcggcgat ccgcgccagg tgctcgcgca ggtgctggcc ggctacacgg cgctcgggct
     2821 gacgccggtc gtggcgctgg agctggagtt ctacctgctc gatcgcgagc cctcgccgga
     2881 tggctcgccg cagccgccgc tgtcacccgg caccggccgc cggcagtgga gttcgcaggt
     2941 ctacggcatc gacgagctgc acgagttcga ggacttcttc gacggcgtcg cccgcgcctg
     3001 cgtgctgcag cgcctgccgg tcgacaccac ggtcgccgaa caggcgccga accagtacga
     3061 gatcaacctc aagcacgtcg acgatgccat cctcgccgcc gacaacgccg tgctgctcaa
     3121 gcgcgtgatc aagggcgtcg cgcagcagca ccagatggcc gcgaccttca tggccaagcc
     3181 ctatggcgaa ctcgccggca acggcatgca catccacttc agcctgatcg acgagcgcgg
     3241 cttcaacgtc ttcgatgacg gcagcgccga aggcagcgcg ctgctcaagc acgcgatcgc
     3301 cggcatggtc acgaccatgc gcgagacgat ggcgctctac gccccgaaca ccaattccta
     3361 ccgccgcttc cagcccggca gccacgcgcc ggtgtcggcg aactggggct acgacaaccg
     3421 cgctgcggcg ctgcgcattc cgtccggcga gcggcaggcg atgcgcatcg aacaccgtct
     3481 tgccggcgcc gacgccaatc cgtatctggc gctggcggcg atgctggccg ggggcctgcg
     3541 cggcatccgc gagaagctcg atcccggtga gcccagtcgc ggccatgtcg gcaacggcca
     3601 ggcgccggtg ctgccggcct cgctgaagca cgcgctggaa atcttcgaca agtccccgtt
     3661 cccgcgcgaa gccttcggcg actcctaccc gcgcctctac agcgcctgtc gctggcagga
     3721 gctggagaag ttcggcaagc aggtcacgcc gctggaatat gattcgtacc tgagaacggt
     3781 ttgatgcgga tttatggttt gcaggcagct ccggacaggc tttgcggggt cggattccgg
     3841 ttgtcatgac ccgtctgagc agcgaggatg cggaaatggc tcatgaaaag aagattgcac
     3901 acctgacctt cattcagggc atcatcaccc ggatgggcaa taattccttt ttgctcaagg
     3961 gctggaccgt taccttggtt gcggccttgt ttgcacttgc agccaaggat agtaatcaga
     4021 atttcatact gattgcttat tttcccatcc ttgtgttctg gatgctggat ggatatttcc
     4081 tgtatcagga gcgtttgttc agggaccttt acaatggggt tgccaacgac aggatttcca
     4141 gcgaacagtt cgagatcaat cctgccagcc tcatgagcga cgctggtaaa tggcagccag
     4201 cctttctctg ggcacgagct gcgctctcca aaactttact tgtcttttac atggcgatga
     4261 tgggagtgac gctgttcgtc atgtgcttcc tgctgcgttg agcgggcctg atcgaaagag
     4321 cggctgaggt gctggcggcc ggcaggtgcc cggggacggg tggcgtgccg gtcagttttt
     4381 ctcatgcgtg catcaacagt ccttgttgat gtaggtcatg aaaaatcggt aatgatcgtg
     4441 ccaccgtgtg cttgtgaaga cctgcagcaa accggttgct gcggtgccgg
//
LOCUS       DAOQJH010000130         4482 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_11621_length_4482_cov_3.663203, whole genome shotgun sequence.
ACCESSION   DAOQJH010000130 DAOQJH010000000
VERSION     DAOQJH010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 4482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4482
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11621_length_4482_cov_3.663203"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..971)
                     /locus_tag="PLQ18_04225"
     CDS             complement(<1..971)
                     /locus_tag="PLQ18_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HNE00892.1"
                     /translation="MNIALSLLHALKAHGAREIFGIPGDFALPFFRIVEESGVLPLYT
                     LSHEPGIGFAADAAARFNGALGVAAVTYGAGALNMVNPVAGAYAERSPLVVISGAPGA
                     AEVRSGMLLHHQVKTTDSQFAIYREITCAQTRLDDPATAPAEIARVLGACREQSRPVL
                     IEVPRDAVALPCAPVRPAAPAAYDREAVEACAGEILARLAAAARPTLLVDIEVRRYGL
                     EARVAELAHRLAIPVVTTFMGRGLLAEAAVPVAGTYLGVAGEPAVTALVEESDCLLML
                     GVLLSDTNFGVSGRQIDLRKAVYAADRQVQIGFHHYPDLPLGALVEAL"
     gene            1296..2858
                     /locus_tag="PLQ18_04230"
     CDS             1296..2858
                     /locus_tag="PLQ18_04230"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNE00893.1"
                     /translation="MRLQIVFDDRVGIAMAVLNSLARHNINMTAGDIVTQNDGRGSFY
                     LHAAQMTLDELHRVLPEIRQVPGVRDVRPIPLMPGERKQSELRTLLGALPAPVLSLDL
                     EGRVVVANESAQRLLKLKGPDGQGTPLAPLVEDFDLVEHINRLDRPEYGVRLALRGQA
                     VLADFIPVLVPLARAGKSLSGAVMVLHGGSLEVASPAVDAGFASIVHRSAPMRALVAQ
                     ARRMAPLSAPILILGESGSGKARLAYACHLASPRREAPFLRLNAGELADAGAEAVLFG
                     VREQPGLFEQGAGGTLFLSDVAELSPALQARLLGFLQDGRFRRGGSVEPRRADVRIIA
                     STRVDLAAEVRDGRFRDDLYYHLHVLNLQMPSLRERQADVLPVALEAIEMAARQLACA
                     PPRLTPEAEAALLAHGWPGNVRQLENTLLRAVTVAEGGAITPELLALGTDATAASGAP
                     IDEALFEGSLAEALARVERLMLARLYPSHPSTRQLARRLGLSHTAVANKLRQYGIGLG
                     PGSGPAAEPRRG"
     gene            complement(2875..3357)
                     /locus_tag="PLQ18_04235"
     CDS             complement(2875..3357)
                     /locus_tag="PLQ18_04235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE00894.1"
                     /translation="MSEEISRDYVHGVYTELLAREGFRPKTDEDGDIQFRNQGFTTYI
                     RISEDDLPFLQMFIPSVYTLESGEEDTVALAVMAELNRQFKLVKVCRLDHSVAVLVEM
                     LLAQPEDLGGSLLRIADVLVVVAHEFHRLMSAALRERQRQAESELDEDDLPMPTRTVN
                     "
     gene            3695..3919
                     /locus_tag="PLQ18_04240"
     CDS             3695..3919
                     /locus_tag="PLQ18_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE00895.1"
                     /translation="MKTSMIIGVLLLVAGIAVLAYRGFNYTSEETVLQVGPVKATAET
                     EKHVGIPAWAGYTALALGGALVVVGATRRT"
     gene            4186..>4482
                     /locus_tag="PLQ18_04245"
     CDS             4186..>4482
                     /locus_tag="PLQ18_04245"
                     /inference="COORDINATES: protein motif:HMM:NF013460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HNE00896.1"
                     /translation="MWRNSASAYGLTARLLHWSIAVLILGLIPLGWYITTLDYYDPGY
                     RLWPGLHRGFGVVVGLLALVRIGWAIADRAPPLAATLKPWEKLGAHAGHGLLYLA"
BASE COUNT          626 a         1581 c         1592 g          683 t
ORIGIN      
        1 agcgcctcga ccagcgcgcc gagcggcagg tccggatagt ggtggaagcc gatctgcacc
       61 tgccggtcgg ctgcgtacac ggccttgcgc aggtcgatct ggcggccgga gacgccgaag
      121 ttggtgtccg acagcagcac accgagcatc agcaggcagt ccgattcctc gaccagcgcc
      181 gtcaccgccg gctcgccggc gaccccgaga tacgtgccgg cgaccggcac cgcggcctcg
      241 gcgagcaggc cgcggcccat gaaggtcgtc acgaccggaa tcgccagccg gtgcgcgagt
      301 tcggcgacac gtgcctccag cccgtagcgg cgcacctcga tgtcgacgag cagcgtcggg
      361 cgggcggccg cggccaggcg cgcgaggatt tccccggcgc aggcctcgac cgcctcgcgg
      421 tcgtaggcgg ccggcgcggc cgggcgcacc ggcgcacacg gcagcgccac cgcatcgcgc
      481 ggcacctcga tcagcaccgg ccgcgactgc tcgcgacagg cgccgagcac gcgcgcgatc
      541 tcggccgggg ccgtcgccgg atcgtcgagc cgggtctgcg cgcaggtgat ctcgcggtag
      601 atcgcgaact gcgagtccgt cgtcttcacc tgatggtgca gcagcatgcc ggagcgcacc
      661 tcggccgcgc ccggtgcccc ggaaatcacc accagcgggc tgcgctcggc ataggcaccg
      721 gcgaccgggt tgaccatgtt cagcgctccg gcgccgtagg tgacggcggc gacaccgagt
      781 gcgccgttga agcgcgccgc cgcgtcggcc gcgaagccga tgccgggttc gtgcgacagc
      841 gtgtacagcg gcagcacgcc gctttcctcg acgatgcgga agaacggcag cgcgaagtcg
      901 ccgggaatgc cgaagatctc gcgggcgccg tgcgccttca gcgcgtgcag cagggacagg
      961 gcgatgttca tggcgcggcg tggcctccgg gcgcgatcgc cggcacggag gccggacggg
     1021 gatggcgcac aggtagcaga ggccgggtgc gcgctgctgc accgccgtgg cggccacggc
     1081 tgcgggcagg gtccggcgag ctccgggatt gtcaatgatc ggttacaggt aagaaaggaa
     1141 tcgacgaaaa cccgcatgca gtctgacgtt gtgatcggcg cccggcgtca actaatctgc
     1201 tgcggacgta cacacagcca tacgaccctg cggtgtcgtg cgttgccgag gcggccgtgc
     1261 gatgccggat gacctgaatc ccggaaggag acgccatgcg cctgcagatc gtgttcgatg
     1321 accgcgtcgg catcgcgatg gccgtgctga acagtctggc gcggcacaac atcaacatga
     1381 ccgccggcga catcgtcacg cagaacgacg gccgcggttc gttctatctg cacgccgcgc
     1441 agatgacgct cgatgagctg caccgcgtgc tgcccgagat ccgccaggtg ccgggcgtgc
     1501 gcgacgtgcg gccgatcccg ctgatgccgg gcgagcgcaa gcagtcggag ctgcgcacgc
     1561 tgctcggcgc gctgccggcg ccggtgctgt cgctcgatct cgaaggccgg gtcgtcgtcg
     1621 ccaacgaatc ggcgcagcgc ctgctgaagc tcaaggggcc ggacggtcag ggcacgccgc
     1681 tcgcgccgct ggtcgaggac ttcgatctgg tcgagcacat caaccggctc gatcggcccg
     1741 agtacggcgt gcgtctggcg ctgcgcggcc aggccgtgct tgccgacttc attccggtgc
     1801 tggtgccgct cgcacgcgcc ggcaagtcgc tgtcgggggc ggtcatggtg ctgcacggcg
     1861 gcagcctcga agtcgcctcg ccggccgtcg atgccggctt cgcgtcgatc gtgcaccgct
     1921 cggcgccgat gcgtgcgctg gtggcgcagg cccggcgcat ggcgccgctg tcggcgccga
     1981 tcctgatcct cggcgaatcc ggctccggca aggcgcggct cgcctacgcg tgtcacctcg
     2041 ccagcccgcg gcgcgaggcg ccgttcctgc gcctcaacgc cggcgaactc gccgatgccg
     2101 gcgccgaggc cgtgctgttc ggcgtgcgcg agcagccggg cctgttcgag cagggcgccg
     2161 gcggcacgct gttcctgtcc gatgtcgccg agctgtcgcc ggcgctgcag gcgcggctgc
     2221 tcggcttcct gcaggacggg cgcttccggc gcggcggcag cgtcgagccg cgccgcgccg
     2281 acgtgcgcat catcgcctcg acgcgcgtcg atctggcggc cgaggtccgc gacggacgct
     2341 ttcgcgacga tctctactac cacctgcacg tgctgaacct gcagatgccg tcgctgcgcg
     2401 agcggcaggc cgacgtgctg ccggtcgcgc tcgaagccat cgagatggcc gcgcgccagc
     2461 tcgcctgcgc gccgccgcgg ctgacgccgg aggccgaggc ggcgctgctc gcgcacggct
     2521 ggcccggcaa cgtccgccag ctcgaaaaca ccttgctgcg cgccgtgacc gtcgccgagg
     2581 gcggcgcgat cacgcccgag ctgctcgcgc tcggcaccga cgccacggcg gcgagcgggg
     2641 cgccgatcga cgaggcgctg ttcgagggct cgctcgccga ggcgctggcg cgcgtcgagc
     2701 ggctgatgct cgcgcgcctg tatccgagcc atccgagcac gcgccagctc gcgcgccgcc
     2761 tcggcctgtc gcataccgcg gtcgccaaca agctgcgcca gtacggcatc ggcctcggcc
     2821 ccggttccgg ccctgccgcg gagccgcgtc gcggctgagg acgcggctcg tccgtcagtt
     2881 gacggtgcgc gtcggcatcg gcagatcgtc ctcgtcgagc tcggactcgg cctgacgctg
     2941 gcgctcgcgc agcgccgcgc tcatcagccg gtggaattcg tgcgcgacca ccaccagcac
     3001 gtcggcgatg cgcagcagcg agccgccgag gtcctcgggc tgcgcgagca gcatctcgac
     3061 cagcaccgcg accgagtgat cgagccggca gaccttcacg agcttgaact ggcggttcag
     3121 ctcggccatc accgccagcg cgaccgtgtc ctcctcgccg gactccagcg tgtacaccga
     3181 cgggatgaac atctgcagga acggcagatc gtcttcgctg atgcggatgt aggtcgtgaa
     3241 gccctggttg cggaactgga tgtcgccgtc ctcgtcggtc ttcggccgga agccttcgcg
     3301 ggcgagcagt tcggtgtaga cgccgtgtac gtagtcgcgg ctgatttctt cgctcatctg
     3361 accttccttt ccccggtgcg gcgggtgcgg caaccgcagt gcggtcgcac gatggctgga
     3421 ggggattcga tccggatcgg tctgcacccg gtctgcacag tttgccgtgc cggcgcgctg
     3481 cgcgtgtcgc aaaccgtaag gcatgaccgc cggcaatccc ggatgcagtc ctgcagggaa
     3541 ccctgattgt ggcgcagggg tcgatgtccg gtgtgaccgt cacgcgccga aggcgtgcag
     3601 gggacggccc gcagaggtcg accgtgcgac ggtgacaccg ctctccactg cgccttcact
     3661 gcattttcac cgcagccttt caggggtatc cgccatgaag acttccatga tcatcggcgt
     3721 gctgctgctg gtcgccggca tcgccgtgct cgcctaccgc ggcttcaact acaccagcga
     3781 ggagaccgtg ctgcaggtcg gaccggtcaa ggccacggcc gagaccgaga agcacgtcgg
     3841 catcccggcc tgggccggct ataccgcgct ggcgctcggc ggcgcgctgg tggtcgtcgg
     3901 cgcgacgcgc cgcacctgag ccggcctgca tcccggccgc tcgacacggc ggcccggcgt
     3961 tgcggcgccg ggccgccgtt ttttgtgttt ttcgtgactt tgccggcgcg ccgtcgtgcg
     4021 cgcaaaaagg gcaggttcgc aaaagttccg gcggtattgt caatccggag cgaacaatcc
     4081 tgtcacgcaa gcagcgtcgc tgtgccggaa tgcggctgct agcctgctgc gcaactcgaa
     4141 cgacaccgct gccggtgtcc cccgcttgcg cgaggtcaat ccgcgatgtg gcgcaattcg
     4201 gcatccgcct acggcctgac ggcccgcctg ctgcactgga gcatcgccgt gctgatcctc
     4261 ggcctgatcc cgcttggctg gtacatcacg acgctcgatt actacgaccc cggctaccgg
     4321 ctgtggccgg gcctgcaccg cggcttcggc gtcgtggtcg gcctgctggc gctcgtgcgc
     4381 atcggctggg cgatcgcgga tcgcgcgccg ccgctcgcag cgacgctgaa gccctgggaa
     4441 aagctcggcg cgcacgccgg tcacggcctg ctgtatctgg cg
//
LOCUS       DAOQJH010000131         4470 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_11659_length_4470_cov_4.005436, whole genome shotgun sequence.
ACCESSION   DAOQJH010000131 DAOQJH010000000
VERSION     DAOQJH010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 4470)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4470)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4470
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11659_length_4470_cov_4.005436"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(51..956)
                     /locus_tag="PLQ18_04250"
     CDS             complement(51..956)
                     /locus_tag="PLQ18_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007113493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HNE00897.1"
                     /translation="MNYQITHRTTYRYYGRVSLCHNEARLLPRETSRQFVEESALEVL
                     PVPALRRERTDFFGNRVVNFSVQDIHQTLEVTARSRVTVAPRPSLTDTPSPGWEEVVC
                     LVRDLPELGAMEARQYTLESPFIRPNAALAAYARPSFTAGRPLLEAVLELNRRIFKEF
                     TYDPQFSTLATPLSQVLEERRGVCQDFAHLAIGCLRSLGLPARYVSGYLETLPPPGQP
                     RLVGADASHAWLSVFIPGLGWIDLDPTNDLLPDERHVTLAWGRDYADVAPLKGVMIGG
                     GSHALEVSVDVMPLLPPAEADDEAA"
     gene            complement(981..3494)
                     /locus_tag="PLQ18_04255"
     CDS             complement(981..3494)
                     /locus_tag="PLQ18_04255"
                     /inference="COORDINATES: protein motif:HMM:NF025761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HNE00898.1"
                     /translation="MIEPTSFPPAVHDLLSDYAPGSGWDELLDADGKVRPAWEYLLAQ
                     LDALGLGELQARHEETQRLLRENGVTFNAHEDGRAASRAWTLDPIPLPILGDEWAQLE
                     AGLAQRSRLLNRLAADLYGARTVFRRGVLPPELVHAHPGFLHAAWGALPAGARWLVFH
                     AIDFARDEQGRWLVLGDRAQAPSGAGYALENRITLARALPTVYREAPLKRLASFLETE
                     RVTLARLAPRHVDKPNVVLLTPGPASQGYFEHAYLANYLSLSLVQGEDLVVRDGKVSL
                     KTLGGLQPIDVILRRVTDGWCDPLELRGDSMLGVPGLLAAVREGGVVLANALGIGAIE
                     NPGLVPFLPALCRELLGEELLLQPVKSYWCGDGESLEAVLDRLDSLVIHQLTEQTITD
                     GLHLDAAARATLAARIRSAPHLYVAQEHVRPAHAPVWDGRKLVAQPVMMRGFCVAEED
                     GYRVMPGALGAVSPDGRMAVQGGTTKDVWVLAESAQPHVSLLRQAHGPIVVTRDGADL
                     PSRVADNLFWLGRYGERYDSRARLLREALLRLLERGEGAGEDGCLEDLLIALGIELGS
                     VDAGPRGRYLALRRAFLGVFDEQNDGGLREIFAGMLRNGRAVRDHLGDESFRVLNRLR
                     ESLRDLPSPPSASAGRKALEEHLTLLTAFFGLCNETMPHHYGWRFLDIGRFVERVLST
                     LTMLELVFVKTEHPGVPLWEVMLATTDNLTAYRRRYRSALHPSAILDLLLFDEDNPRS
                     VGYLLRRLRRQIERLPHPKATPYRSAEERLILEAQTTLALADIATLAEVTADGDPKGQ
                     RALGKLLRRLRAPLAELSEALSNSHFSHSEKPRQLIEMI"
     gene            complement(3626..>4470)
                     /locus_tag="PLQ18_04260"
     CDS             complement(3626..>4470)
                     /locus_tag="PLQ18_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011390311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HNE00899.1"
                     /translation="LYSPDSATGRLGLVEFRAFEMPPHARMSLLQQLLLRSLVARFWE
                     DPYQGRLCRWGTSLHDRWMLPHFLVEDLKDVLADLDRHGLRLDPEWFAPFSEFRFPVY
                     GRIVQQDIQLELRQAVEPWHVLGEEAVMGGTARYVDSSVERMQVKVEGLNGDRYVLAC
                     NGRRVPLAATGRQGEFVAGVRYRAWQPPSALHPTIPVHAPLVFDLVDTWNQRSIGGCT
                     YHVSHPAGRNFVVFPVNANEAEARRLARFWAYGHTPNAMQAPAEERSLDLPMTLDLRR
                     RPGC"
BASE COUNT          665 a         1541 c         1644 g          620 t
ORIGIN      
        1 cggttcggct ggaaccgccg atggtacacc cgctccgcca gagcaggact tcaggccgcc
       61 tcgtcgtcgg cctccgccgg cggcagcagc ggcatcacgt cgaccgacac ttcgagcgcg
      121 tgcgaaccgc cgccgatcat gacgcccttc agcggcgcga catcggcgta gtcacgtccc
      181 caggcgagcg tcacgtggcg ttcgtcgggc agcaggtcat tggtcggatc gagatcgatc
      241 cagccgaggc cggggatgaa caccgagagc caggcgtgcg aggcgtcggc cccgaccagc
      301 cgcggctggc ccggcggcgg cagggtctcc agatagccgc tgacgtaacg cgccggcagg
      361 ccgagcgagc gcaggcagcc gatggccaga tgcgcgaagt cctgacagac gccgcggcgc
      421 tcctccagca cctgcgacag cggcgtggcc agcgtcgaga actgcgggtc gtaggtgaat
      481 tccttgaaga tgcgccggtt cagttcgagc acggcctcca gcaagggccg gccggccgtg
      541 aacgacggcc gcgcataggc ggcgagcgcg gcgttcgggc ggatgaacgg cgattcgagc
      601 gtgtactggc gcgcttccat cgcgccgagc tcgggcaggt cgcgcaccag gcagacgacc
      661 tcctcccagc ccggcgacgg cgtgtcggtc agcgacgggc gcggggcgac cgtcacgcgc
      721 gagcgcgccg tgacctccag cgtctggtgg atgtcctgca ccgagaagtt gacgacgcgg
      781 ttgccgaaga agtcggtgcg ctcgcgccgc agtgccggca ccggcaggac ctccagcgcc
      841 gattcctcga cgaactggcg gctggtctcg cgcggcagca gccgggcttc gttgtggcac
      901 agcgacacgc gcccgtagta gcggtaggtc gtgcggtgcg tgatctgata gttcatcgct
      961 gggcttccgt ggcgcggccg tcagatcatc tcgatcagct ggcgcggctt ctcggaatgg
     1021 ctgaagtggc tgttggaaag cgcctcggac agctcggcga gcggagcgcg cagccggcgc
     1081 agcagcttgc cgagcgcgcg ctggcccttc ggatcgccgt cggcggtgac ctcggccagc
     1141 gtcgcgatgt cggcgagcgc gagcgtggtc tgggcttcga ggatcagccg ctcctcggcg
     1201 ctgcgatacg gcgtggcctt cgggtgcggc aggcgctcga tctggcggcg cagccggcgc
     1261 agcagatagc cgaccgagcg cgggttgtcc tcgtcgaaca gcagcaggtc gaggatcgcg
     1321 ctcgggtgca gcgccgagcg gtagcggcgg cggtaggcgg tcaggttgtc ggtggtcgcg
     1381 agcatgacct cccacagcgg cacgcccggg tgctcggtct tcacgaacac cagttcgagc
     1441 atcgtcagcg tgctcagcac gcgctcgacg aagcggccga tgtcgaggaa gcgccagccg
     1501 tagtggtgcg gcatggtctc gttgcagagg ccgaagaagg ccgtcagcag cgtcaggtgt
     1561 tcctccagcg ccttgcgccc ggcgctcgcg ctcggcggac tcggcaggtc gcgcaggctc
     1621 tcgcgcaggc ggttcagcac gcggaaggat tcgtcgccga ggtgatcgcg cacggcgcgg
     1681 ccgttgcgca gcatgccggc gaagatctcg cgcagcccgc cgtcgttctg ctcgtcgaac
     1741 acgccgagga aggcccggcg cagcgccaga tagcgcccgc gcggaccggc gtcgacgctg
     1801 ccgagctcga tgccgagcgc gatcagcagg tcttcgagac agccgtcctc gccggcgccc
     1861 tcgccgcgtt cgagcaggcg cagcagcgcc tcgcgcagca gccgggcgcg gctgtcgtag
     1921 cgctcgccgt agcgcccgag ccagaacagg ttgtcggcca cccggctcgg caggtcggcg
     1981 ccgtcgcgcg tgaccacgat cgggccgtgc gcctggcgca gcaggctcac gtgcggctgc
     2041 gccgattcgg cgagcaccca gacatccttg gtcgtgccgc cctgcacggc catgcgcccg
     2101 tcggggctga ccgcgccgag ggcgcccggc atcacgcggt agccgtcttc ctcggccacg
     2161 cagaagccgc gcatcatcac cggctgcgcg accagcttgc gcccgtccca gaccggcgcg
     2221 tgcgccgggc gcacgtgctc ctgggcgacg tacaggtgcg gcgcgctgcg gatgcgcgcg
     2281 gccagcgtgg cgcgggcggc ggcatcaagg tgcaggccgt cggtgatcgt ctgttcggtg
     2341 agctggtgga tgaccaggct gtcgagccgg tcgagcacgg cttcgaggct ttcgccgtcc
     2401 ccgcaccagt agctcttgac cggctgcagc agcagttcct cgccgagcag ctcgcgacag
     2461 agcgccggca ggaagggtac gagtccgggg ttttcgatcg cgccgatgcc gagcgcgttg
     2521 gccagcacca cgccgccctc gcgcaccgcc gccagcagcc ccggcacgcc gagcatcgaa
     2581 tcgccgcgca gttcgagcgg atcgcaccag ccgtcggtga cgcggcgcag gatcacgtcg
     2641 atcggctgca ggccgccgag cgtcttcagc gagaccttgc cgtcgcgcac gaccagatcc
     2701 tcgccctgca ccagcgacag cgacaggtag ttggcgagat acgcgtgctc gaagtagccc
     2761 tggctcgccg gtccgggcgt cagcagcacg acgttgggct tgtcgacgtg gcgcggcgcc
     2821 agacgcgcga gcgtcacgcg ttcggtttcg agaaagctcg caaggcgctt cagcggcgcc
     2881 tcgcggtaga cggtcggcag cgcccgcgcg agcgtgatgc ggttttccag cgcatagccg
     2941 gcgccggacg gtgcctgggc gcgatcgccg agcaccagcc agcggccctg ctcgtcgcgc
     3001 gcgaagtcga tggcgtggaa caccagccag cgcgccccgg ccggcagcgc cccccaggcc
     3061 gcgtgcagga agcccggatg ggcatgcacg agttcgggcg gcagcacgcc gcggcgaaac
     3121 accgtgcgtg cgccgtagag atcggcggcg aggcggttca gcaggcggct gcgctgcgcg
     3181 agccccgcct ccagctgcgc ccattcgtcg ccgaggatcg gcagcgggat cgggtcgagc
     3241 gtccacgcgc ggctcgcggc gcggccgtcc tcgtgggcgt tgaacgtgac gccgttctcg
     3301 cgcagcagcc gctgggtttc ctcgtggcgg gcctgaagct ccccgaggcc gagcgcatcg
     3361 agctgggcga gcaggtattc ccaggccgga cgcaccttgc cgtcggcatc gagcagctcg
     3421 tcccagccgc tgcccggcgc gtagtcgctc agcaggtcgt ggaccgccgg cgggaaactg
     3481 gtcggctcga tcatggcctg cgcgtaccgg gtgactgaaa cggccgtcta tcgtagcgcg
     3541 ccacgaccgc ggacggcgcc ccctaccccc gatgttccgg gagccgggtg cgccgccgcg
     3601 gccggcgcgt cgtggtccag ccgcctcagc agcctggacg acggcgcagg tcgagcgtca
     3661 tcggcaggtc gaggctgcgt tcctcggccg gcgcctgcat cgcgttcggc gtgtggccgt
     3721 aggcccagaa gcgggccaga cggcgcgcct cggcttcatt ggcgttgacc gggaacacga
     3781 cgaagttgcg accggccggg tgcgagacgt gatacgtgca gccaccgatc gagcgctggt
     3841 tccaggtatc gacgaggtcg aagaccagcg gcgcgtgcac cgggatggtc ggatgcagcg
     3901 cggacggcgg ctgccaggcg cgatagcgca cgccggcgac gaactcgccc tgacgaccgg
     3961 tggccgcgag cggcacgcgc cggccgttgc aggcgagcac gtagcgatcc ccgttgaggc
     4021 cttcgacctt gacctgcatg cgctcgaccg aggaatcgac gtagcgcgca gtaccgccca
     4081 tgacggcttc ctcgccgagc acgtgccagg gctcgacggc ctgacgcagt tcgagctgga
     4141 tgtcctgctg cacgatgcgc ccgtagaccg ggaagcggaa ctccgagaac ggcgcgaacc
     4201 attccggatc gaggcgcagg ccgtgacggt cgaggtcggc gagcacgtcc ttgaggtcct
     4261 cgaccaggaa gtgcggcagc atccagcgat cgtgcaggct ggtgccccag cggcacaggc
     4321 ggccctgata tggatcttcc cagaagcgcg cgaccagcga gcgcagcagc agctgctgca
     4381 gcaggctcat gcgcgcgtgc ggcggcatct cgaaggcgcg gaactcgacc agaccgaggc
     4441 ggccggtcgc cgaatccggc gagtacagct
//
LOCUS       DAOQJH010000132         4458 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_11701_length_4458_cov_3.880763, whole genome shotgun sequence.
ACCESSION   DAOQJH010000132 DAOQJH010000000
VERSION     DAOQJH010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 4458)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4458)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4458
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11701_length_4458_cov_3.880763"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1089)
                     /locus_tag="PLQ18_04265"
     CDS             complement(<1..1089)
                     /locus_tag="PLQ18_04265"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018717796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HNE00900.1"
                     /translation="MPIPLAASLSRRRAQTLRSLLEVPGPPLIIGVAGDSSAGKTTYT
                     NGLRRMLGDDIVGTIEMDGYHKEDRATRARTGRSPLDPEMNHLGLLREHLEALRRGES
                     VHLRRYDHGTGTFIAPQPFSPKPILVLEGLHALYPEFTSLLDYTVFVDPARDIKLGWK
                     IARDTQFRGHRVEPLADEIRKREAAFRRWIEFQKTDANVVIKINRSKLKLLANEEYWG
                     DRPPEGCFHMELIFQPAHTPLPPLRMSFSLAKMLGEDPLPFALAVVPRSYWGRRANAI
                     HLDGLMPPRAVHVLQGQIERFTGIEVTDSHLSGIRKHERMPAIVFTQLLVTWPILEHI
                     ANLLEKRAASAGVTPVSRIEPQDSDDELP"
     gene            complement(1388..2038)
                     /locus_tag="PLQ18_04270"
     CDS             complement(1388..2038)
                     /locus_tag="PLQ18_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018609205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HNE00901.1"
                     /translation="MSATMSSVRPTNTAPTGLKPLVFLLCLLPLGLLIWRGFEAGLGA
                     NPVETLEHATGDWALRLLLLTLCATPLRQLSGWSWPLRLRRMLGLYTFFYASVHMALY
                     FTLDLELDFYMLGNEVLKRPYLSVGFLAWALLIPLALTSTDRMIRRLGRRWKSLHRSV
                     YVIGVLAVLHYLWLVKADVREPLLYGAILIVLLGWRAWRERRDRKGGRRSQAVRTV"
     gene            2208..>4458
                     /locus_tag="PLQ18_04275"
     CDS             2208..>4458
                     /locus_tag="PLQ18_04275"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HNE00902.1"
                     /translation="MFRPNPCPQRARGALRVLAIVLLLIGLGTPLSLPAQTDPPPPRA
                     LADLLNDWKATLDGLGRQLAAGRSEEADLEKIRTEAERVRSAVQASRGEALGKVAAAQ
                     QLVEALGPPPAEGQPPEAKDVARQRQQLGADLGAAEGRAKQLDVLEARANGLINDASR
                     AIRERIASSLLARGPSTLEADVWSLGGTQFALGLQQLSAGPVARWQSNGFAPLFAKSA
                     GTLFVLAIGLAAAWWLRRWLLARYGRNDIEAAPSYSRRVGAALAEGCCRALLPSLAAA
                     AVALTLHVTGLVEAPGRPMLIGFTVAVIFYVLVTGIDSAALAPSAPAWRLTGLDDRSA
                     RALSRRLNLIAAVFAPLLLASLAAPALNLAEEGRALVVCIGSVMLGLALLTVTRRDTW
                     QRTEGEAPAVEAADALDPLAGQRPEGEAAPNRSTLAEPFWALMRFAVALIAVAAPVAS
                     LLGYVNLARYLLENLVWTALGLAGFVIVRRLIGEAVDRLVLVEGAHRGPLARMFALKA
                     GSSQTLAFWLMLLIDLTLLALAGVLLLLRWGVRWADIQNGLNALLYGFKLGGMTLSLL
                     DGLLAMALFAAVLAASRVSQRVLEERVLPHTHLDAGLRNSLKSGIGYVGLAIAIAVAV
                     STLGIDLSKAALLAGALSVGIGFGLQNIVSNFVSGLILLAERPIKVGDWVVVGTNEGT
                     VRRINVRATELETSQRASVIIPNSMLLSQAVLNWTHKDTFGRVEILVGVAYESDPEAV
                     QRILLDCASV"
BASE COUNT          641 a         1472 c         1635 g          710 t
ORIGIN      
        1 cgggagttca tcgtcgctgt cctggggctc gatgcgcgag accggcgtca cgccggcgga
       61 ggccgcgcgc ttttcgagca ggttggcgat gtgttcgagg atcggccagg tcaccagcag
      121 ctgcgtgaac acgatcgccg gcatgcgctc gtgcttgcgg atgccggaga ggtgcgagtc
      181 ggtgacctcg atgccggtga agcgctcgat ctggccctgc agcacgtgca cggcgcgcgg
      241 cggcatcagg ccgtcgaggt ggatggcgtt ggcgcgccgc ccccagtacg agcgcggcac
      301 gacggccagc gcgaacggca gcgggtcctc gccgagcatc ttggcgaggc tgaaggacat
      361 gcgcagcggc ggcagcggcg tgtgcgccgg ctggaagatc agctccatgt ggaagcagcc
      421 ttccggcggc cggtcgcccc agtattcctc gttggcgagc agcttcagct tgctgcggtt
      481 gatcttgatg acgacgttgg cgtcggtctt ctggaactcg atccagcggc ggaacgccgc
      541 ttcgcgcttg cggatctcgt cggccagcgg ctcgacgcga tgcccgcgga actgcgtgtc
      601 gcgcgcgatc ttccagccga gcttgatgtc gcgggccgga tcgacgaaca ccgtgtaatc
      661 gagcagtgac gtgaactcgg gatacagcgc gtgcaggcct tcgagcacga ggatcggctt
      721 cggcgagaac ggctgcggcg caatgaaggt gccggtgccg tgatcgtagc ggcgcaggtg
      781 cacggattcg ccgcggcgca gcgcttcgag gtgctcgcgc agcaggccga ggtggttcat
      841 ctcgggatcg agcggcgaac ggccggtgcg cgcgcgcgtg gcgcggtcct ccttgtggta
      901 gccgtccatc tcgatcgtgc cgacgatgtc atcgccgagc atgcgccgca gaccgttggt
      961 gtaggtggtc ttgccggcac tgctgtcgcc ggcgacgccg atgatcagcg gcggaccggg
     1021 tacttcaagc agggagcgca gcgtctgggc gcggcgccgc gaaagggacg cggcgagcgg
     1081 aatgggcatc agcgactccg tagcgtcggt atcgccggac agtgtggaca gcggggcggg
     1141 gggggatcaa ggccgcgacc ctggaagtcg ggcaaaacaa cgggggaaga ccttgcggcc
     1201 ttccccacca aacaagacgg tacttccgtt gtacttggca aacctgcccg cagcgggaac
     1261 ggacagtcgc ggttttaata gaagcaaatg atgggccagt ttataaaaaa ctttattttc
     1321 aattacttat tatgtttcgc ccgggcttgc cgtaaggaat cctgacaatg tgatgccgct
     1381 cgccggttca gacggtgcgc accgcctgcg agcgccggcc gcccttgcgg tcccgccgtt
     1441 cccgccacgc gcgccagccg agcagcacga tcaggatcgc gccgtacagc agcggctcgc
     1501 gcacgtcggc cttgaccagc cacaggtagt gcagcacggc gagcacgccg atcacgtaga
     1561 cgctgcggtg caggcttttc cagcgccggc cgaggcggcg gatcatgcgg tcggtcgagg
     1621 tcagcgccag cggtatcagc agcgcccagg ccaggaagcc gacgctcagg tagggacgct
     1681 tcaggacttc gttgccgagc atgtagaaat cgagttcgag atcgagcgtg aagtacagcg
     1741 ccatgtgcac gcttgcatag aagaacgtgt acaggccgag catgcgccgc agccgcagcg
     1801 gccagctcca gccgctgagc tggcgcagcg gtgtcgcgca cagcgtcagc agcagcaggc
     1861 gcagcgccca gtcgccggtg gcgtgttcca gggtctcgac cggattggcg ccgagcccgg
     1921 cctcgaagcc gcgccagatc agcaggccga gcggcagcag gcagagcagg aacaccagcg
     1981 gcttgaggcc ggtcggggcg gtgttcgttg ggcggacaga gctcatcgtg gcggacaagg
     2041 ggcggattcc tgcagcaagg gcgttaccgg atggtacggg cggcaaggcc gtgcggatgc
     2101 agcggcccgg gctgtggcgg gggcgccgtc gtcatccgcg tgcggcaagt gcttgtgcta
     2161 gcgtatgtgc ctttgtctga aagcccgtca ttgccgagcg ttccttcatg ttccgtccga
     2221 atccctgccc gcagcgcgcc cggggtgcgc tgcgagtcct cgccatcgtc ctgctgctga
     2281 tcggcctggg cactccgctg tcgctgcccg cgcagaccga tccgccgccg ccgcgcgcgc
     2341 tcgcggacct gctcaacgac tggaaggcca cgctcgatgg cctcggccgg cagctcgccg
     2401 ccggcaggag cgaggaagcc gatctcgaga agatccgcac cgaggccgag cgcgtgcgca
     2461 gcgccgtgca ggccagccgc ggcgaggcgc tcggcaaggt ggccgccgcg cagcagctgg
     2521 tcgaggccct cgggccgccg ccggccgagg gccagccgcc cgaggcgaag gacgtggctc
     2581 gccagcgcca gcagctcggc gccgatctcg gcgccgccga aggccgggcc aagcagctcg
     2641 acgtgctcga agcgcgcgcc aacggcctga tcaacgacgc ctcgcgcgcg attcgcgaac
     2701 gcatcgcatc gagcctgctg gcgcgtggcc cgagcacgct cgaagccgat gtctggtcgc
     2761 tcggcggcac gcagttcgcg ctcggcctgc agcagctttc ggccggtccg gtggcgcgct
     2821 ggcagtccaa cggcttcgca ccgctgttcg cgaagagcgc cggcacgctg ttcgtgctcg
     2881 cgatcgggct ggcggcggcc tggtggctgc ggcgctggct gctggcgcgc tacgggcgca
     2941 acgacatcga ggcggccccg agctacagcc gccgcgtcgg ggcggcgctg gccgagggct
     3001 gctgccgggc gctgctgccg agtctggcgg cggccgcggt cgcgctgacg ctgcacgtga
     3061 ccgggctggt cgaggcaccg ggccggccga tgctgatcgg cttcacggtc gcggtgatct
     3121 tctacgtgct ggtcaccggc atcgacagcg ccgcgctggc gccttcggct ccggcctggc
     3181 ggctgaccgg cctcgacgat cgcagcgccc gggccttgtc gaggcggctg aacctgatcg
     3241 ccgcggtgtt cgcaccgctg ctgctcgcgt cgctggcggc cccggcgctg aacctcgccg
     3301 aggaggggcg ggcgctggtg gtgtgcatcg gcagcgtcat gctcgggctc gcgctgctga
     3361 cggtcacgcg ccgcgatacc tggcagcgca ccgagggcga ggcgccggcg gtcgaggccg
     3421 ccgatgcgct cgatccgctg gccggccagc gcccggaggg cgaggcggca ccgaatcgct
     3481 cgacgctggc cgagccgttc tgggcgctga tgcgctttgc ggtcgcgctg atcgcggtcg
     3541 ccgcgccggt tgcctcgctg ctcggctacg tcaacctcgc gcgctatctg ctcgaaaacc
     3601 tggtctggac cgcgctcggg ctggccggct tcgtgatcgt gcgccggctg atcggcgaag
     3661 ccgtcgaccg gctggtgctg gtcgagggtg cgcatcgcgg gccgctggcg cgaatgttcg
     3721 cgctgaaggc cggcagctcg cagacgctgg ccttctggct gatgctgctg atcgacctga
     3781 cgctgctcgc gctcgccggc gtgctgctgc tgctgcgctg gggcgtgcgc tgggccgaca
     3841 tccagaacgg cctgaatgcg ctgctgtacg gcttcaagct cggcggcatg acgctgtcgc
     3901 tgctcgatgg cctgctcgcc atggcgctgt tcgcggcggt gctcgccgcg agccgcgtca
     3961 gccagcgcgt gctggaggag cgcgtgctgc cgcacaccca cctcgacgcc ggcctgcgca
     4021 actcgctcaa gagcggcatc ggctacgtcg ggctcgcgat cgcgatcgcg gtggcggtct
     4081 cgacgctcgg catcgacctg tcgaaggccg cgctgctcgc cggcgcgctg tcggtcggca
     4141 tcggcttcgg tctgcagaat atcgtcagca acttcgtctc ggggctgatc ctgctcgccg
     4201 agcggccgat caaggtcggc gactgggtcg tggtcggcac caacgagggc acggtgcggc
     4261 gcatcaacgt gcgggcgacc gagctcgaaa cctcgcagcg cgcctcggtg atcatcccga
     4321 actcgatgct gctgtcgcag gcagtgctga actggacgca caaggacacc ttcggccgcg
     4381 tcgagattct ggtcggcgtc gcctacgaat ccgaccccga ggccgtgcag cgcatcctgc
     4441 tcgactgtgc gagcgtgc
//
LOCUS       DAOQJH010000133         4448 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_11752_length_4448_cov_4.366720, whole genome shotgun sequence.
ACCESSION   DAOQJH010000133 DAOQJH010000000
VERSION     DAOQJH010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 4448)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4448)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4448
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11752_length_4448_cov_4.366720"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1257)
                     /locus_tag="PLQ18_04280"
     CDS             complement(<1..1257)
                     /locus_tag="PLQ18_04280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE00903.1"
                     /translation="MTARDLQAARQALRWGVGQTSLKQRVLPPEQDVMPIPGRVGVAP
                     SPADRQTDPPPLDRGFVITAFGAASGARYSPYTLEQLTWPYALANASYGVQPFATSFN
                     IHDTLLWHDVLAFSGGKIWINAATRAALEIPVALVTADATPYLIPSAEPGEPYGSSTL
                     NASQKRVFAVARSTVRTFVPGAPESTLQPVAPRTDRHAVTLGPVVAPASHHATLAQLY
                     HTLPDWKTPGVDWVCSWSIVQMALAAPVLTRTDGSASIMRQPPSFSSPTSSSATTNVA
                     KTFPSTEIMLTGNGQVHRYAIQPNAWDNGAIVIFPWAGTVSAPLAGNERRTFSRRSYA
                     ANSSAQVSVAGTPLTYTASAVRTVDTGTERHVVNGQTVVVAANAVIHPHETLVAAGPV
                     CRWSSGITPAAYAGAAEGWAAYQPSKA"
     gene            complement(1254..1439)
                     /locus_tag="PLQ18_04285"
     CDS             complement(1254..1439)
                     /locus_tag="PLQ18_04285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE00904.1"
                     /translation="MSDAAAPPGGDPLDLVRALDALDSGGRQTSATAKRLPADTPAPA
                     IPTRVGRAESNRPVQAP"
     gene            complement(1436..3076)
                     /locus_tag="PLQ18_04290"
     CDS             complement(1436..3076)
                     /locus_tag="PLQ18_04290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE00905.1"
                     /translation="MAGEIYPATSGTSGVGARAGIWTVIVTIDGVDQTARVIGDLRID
                     AEEGAARIADLTIRPPDGQVVTVAAWVGKSITIDIADQSSGSPTDIQRLFSGLIDTPA
                     LDLETRTIALRCTDNLQNLVAALDNAAIDALTPGGWHSPAVFDPAATGWGRLQDRLST
                     RPAACERSIAGALRVTPWAPALVPHLSFTAEHVLDGSLSVALAARHQLVNEVGISFGY
                     RFPRVKAEAYPIAYQYVAEGNLADHAANLNYWLRRDAVVKAIESAGGTVESITYTALP
                     NQQIGQWIPSAVDYQLCMGFSAEVSFDYVQMIEERHTITVAAPASVAAVGTLRRTLQG
                     ALEGVYPPTQTVEHDMLLFKNKISSIPPLDTATPLTGYTVAADVTLTPETDRAAAGAA
                     MQTLIEIAKTNIWGSHRQNRVRATVALNPAIDLDKTIDIAAGPVHARGKCVSVSHLLS
                     PPTGQATSELAIALCTIAGTGISHPETPTAAPAGSAPATTPLAGEPTADFNFLPEEDH
                     MFTVTFPGVEEVERAKAIIPLASSYNATLSEDILEVTL"
     gene            complement(3084..3608)
                     /locus_tag="PLQ18_04295"
     CDS             complement(3084..3608)
                     /locus_tag="PLQ18_04295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE00906.1"
                     /translation="MTLRTAKIGPVELPLLGALDLDISEQWFGGETTLRTISGAGIKQ
                     VAWHKLRLTISGRGWIPPGLAGLDTSVQHVVASCVPSAITADANRQATLPATRRSDLG
                     YEPWAWALMPDGSTINADLTLVGSLATAAVVSGAQGYQILYYPQITAWISRPDIAGSR
                     ATAGYQWQIVAEQV"
     gene            complement(3605..>4448)
                     /locus_tag="PLQ18_04300"
     CDS             complement(3605..>4448)
                     /locus_tag="PLQ18_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015461980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE00907.1"
                     /translation="RNAADEATANALYAKMAAQHGRAKNAEALADKALRDAETARKYA
                     DKIADPEDRAKAIERISDAQATAEEAKAKNEEARKQALEEQAAGQANTLNELEAQIKA
                     ISEAATKIQVDLQISEALANVASLKAELDTLQDKTVTVTVNTVQTGEGGAAVTAGEPV
                     ATFAQGGYTGRGARLAAAGIVHRGEYVLPQNVVRQPGMLGFLEHLRRYGASALPGFAD
                     GGLVGRLALPTIRPPAPAPTMRPVTLVMPGGSSYGASMGAGDVARLQQDIFARAALAK
                     GGAR"
BASE COUNT          655 a         1486 c         1563 g          744 t
ORIGIN      
        1 tgccttgctg ggctggtacg cggcccaacc ctccgccgcg ccggcgtagg ccgcaggcgt
       61 tatcccggac gaccagcggc aaactggacc ggccgcaacc agcgtctcgt gcggatgaat
      121 cactgcattt gctgcgacga caacggtttg cccattgacc acatgtcgct cggtgcccgt
      181 atcgacggtg cgcacggcgg acgcggtata cgtcagcggc gttccggcga cgctcacctg
      241 cgccgacgaa ttggcggcat acgagcgtct ggaaaatgtg cgccgttcgt ttccggccag
      301 cggcgcgctg accgttccgg cccacgggaa aatgacgata gcgccattgt cccacgcatt
      361 tggctggatg gcatacctgt ggacctgccc gttaccggtg agcatgatct cggtggatgg
      421 aaacgttttg gcgacgttcg tcgtcgcgga cgacgatgtc ggcgaagaga agctgggcgg
      481 ctggcgcatg atcgacgccg acccgtccgt gcgcgtcagg accggggcgg cgagcgccat
      541 ctgaacgatc gaccacgagc aaacccaatc gacgcccggc gttttccaat ccggcagcgt
      601 gtgatacagc tgtgccagcg tggcatgatg actggccgga gcgacgaccg ggccgagcgt
      661 gacggcgtgc cgatcggttc gcggcgccac cggctgcagc gtcgactccg gcgcaccggg
      721 aacgaacgtg cggacggtcg atctggcgac ggcgaaaacc cgcttctggc tggcgttcag
      781 cgtcgagctg ccgtaaggct cgcccggctc ggcgctgggg atgaggtacg gcgtcgcatc
      841 ggccgtgacg agcgcgactg ggatttccag cgcggcgcgg gtcgcggcgt tgatccagat
      901 tttccctccg gaaaacgcca gcacatcgtg ccagagcagg gtgtcgtgaa tgttgaaact
      961 cgtggcgaac ggctgcacgc cgtagctggc gtttgccagc gcgtacggcc aggtgagctg
     1021 ctccagcgtg tacgggctgt agcgcgcgcc ggaagccgcg ccgaacgccg tgatgacgaa
     1081 gccgcgatcg agcggcgggg gatccgtctg cctatcggcc gggctaggcg cgacgccgac
     1141 ccggccgggg atgggcatca cgtcctgctc gggcggcagc acccgctgct tgagcgaggt
     1201 ctgcccgacg ccccaacgca gcgcctggcg cgcggcctgc aaatcgcgcg cagtcatggc
     1261 gcctgcacgg gccgattgga ctcggcgcgc ccgacgcggg tcggaatcgc cggcgcaggc
     1321 gtgtcggccg gcaggcgctt ggccgtcgcg ctcgtctgcc ggccgccgga gtcgagcgca
     1381 tcgagcgcgc gcaccaggtc caacggatcg ccgccgggcg gcgcggcagc atcgctcaca
     1441 gcgtgacctc cagaatgtcc tcgctgagcg tcgcgttgta cgacgaggcg agcgggatga
     1501 tggccttggc gcgctcgact tcctcgacgc cagggaatgt tacggtgaac atgtggtctt
     1561 cctccggcag gaaattgaaa tccgccgtcg gctcgccggc aagcggcgtt gtcgccggcg
     1621 ccgagccggc cggcgcggcc gttggcgttt ccggatggct gatgccggtg ccggcgatcg
     1681 tgcacagcgc gatggccaat tcgctggttg cctgcccggt cggcggcgaa agcagatgag
     1741 agaccgaaac gcacttgccg cgcgcatgca ccggcccggc ggcgatgtcg atcgtcttgt
     1801 cgagatcgat cgccggattg agcgccaccg tggcacgcac ccggttctgc cggtgagatc
     1861 cccagatgtt ggttttcgcg atctcaatca gcgtctgcat ggcggcgccg gcggcggcgc
     1921 gatcggtttc cggcgtcagc gtcacgtcgg ccgccaccgt gtagccggtg agcggcgttg
     1981 ccgtgtccag cggcgggatg ctggaaatct tattcttgaa cagcagcatg tcgtgctcga
     2041 ccgtctgcgt cggcgggtaa acgccttcga gcgcgccctg cagcgtgcgg cgcagcgtgc
     2101 cgacggctgc gacgctggcc ggcgcagcaa cggtgatcgt gtgccgctcc tcgatcatct
     2161 gcacgtagtc gaaggacacc tcggcggaga accccatgca gagctgataa tcgaccgcgc
     2221 tcggaatcca ctggccgatt tgctgattcg gcagcgccgt gtaggtgatc gactcgacgg
     2281 tgccgccggc cgactcgatc gctttgacca ccgcatcgcg ccgcagccag tagttgaggt
     2341 tcgctgcatg gtcggcgagg tttccttcgg cgacgtactg gtaggcgatc gggtacgcct
     2401 cggccttcac gcgcgggaag cggtagccaa agctgatgcc gacctcgttg accagttgat
     2461 ggcgcgcggc cagcgcaacg gagagcgagc catccagcac gtgctcggcg gtgaatgaca
     2521 gatgcggaac cagcgccggc gcccacgggg tcacgcgcag cgcgccggca atgctgcgct
     2581 cgcaggcggc cgggcgcgtc gaaagccggt cctgcagccg cccccagccg gtggctgccg
     2641 ggtcgaagac cgccggcgag tgccagccgc ccggcgtcag cgcatcgatg gcggcgttat
     2701 cgagcgcggc gacgaggttc tgcaggttgt cggtgcagcg cagggcgatc gtgcgcgttt
     2761 ccagatcgag cgccggcgtg tcgatcaggc cggagaacag ccgctgaatg tcggtcggcg
     2821 aaccgctcga ctggtcggca atgtcgatgg tgatggactt gccgacccat gccgcgacgg
     2881 tgacgacctg gccatccggc ggccggatcg tcaaatcggc gatgcgcgcc gcgccctctt
     2941 cggcgtcgat ccgcaaatcg ccgatgacgc gcgccgtctg atcgacgcca tcgatcgtga
     3001 cgatgaccgt ccagattccg gcgcgcgcgc cgacgccgga ggtgccgctg gttgcagggt
     3061 agatttcgcc ggccatgtcg cgctcagacc tgctcggcga cgatctgcca ctggtagccc
     3121 gccgtcgctc gcgacccggc gatgtccggg cgcgaaatcc atgcggtaat ctgcgggtag
     3181 tacaggatct gatagccctg cgcgccgctg acgacggccg ccgtggcgag cgagccgacg
     3241 agcgtcagat cggcattgat cgtgctgccg tctggcatca gcgcccaggc ccagggttcg
     3301 taccccaaat cgctgcgccg cgttgccggc agcgtcgcct gccggttggc gtcggcagta
     3361 atcgccgacg gcacgcatga cgcgacgacg tgctgcacgg acgtatccag cccggccagg
     3421 ccgggcggaa tccagccgcg gccggagatc gtcagccgca gcttgtgcca cgcgacttgc
     3481 ttgatgccgg cgccggagat ggtgcgcagc gtcgtttcgc cgccgaacca ctgttcggag
     3541 atgtcgagat cgagcgcgcc gagcagcggg agttcgaccg ggccgatttt ggcggtgcgc
     3601 aaggtcatcg cgcgcccccc ttggccagcg ccgcgcgcgc gaagatgtcc tgctgcagcc
     3661 gggcgacatc gccggcgccc atcgaagcgc cgtaggacga cccgcccggc atgaccagcg
     3721 tgaccgggcg catcgtcggc gccggcgccg gcgggcgaat cgtcggcagc gccagccggc
     3781 cgaccaggcc gccgtcggcg aaccccggca gcgccgaagc gccgtagcgg cgcagatgtt
     3841 cgagaaaccc gagcatgccg ggctggcgca ccacattctg cggcagcacg tactcgccgc
     3901 gatgcacgat gccggccgcc gccagccgcg ccccgcggcc ggtgtagccg ccctgcgcga
     3961 atgtggcgac cggctcgccg gcagtgacgg ccgcgccgcc ctcgccggtt tgcacggtgt
     4021 tgaccgtgac cgtcacggtc ttgtcctgca gagtgtcgag ctcggccttc aggctggcga
     4081 cattggccag cgcttcggaa atctgcagat cgacctgaat tttggtggcg gcttcgctga
     4141 tcgccttgat ttgcgcttcc agctcgttga gcgtattcgc ctgcccggcg gcctgctctt
     4201 cgagcgcctg cttgcgcgcc tcttcgtttt tcgccttggc ctcttcggcc gtcgcctgcg
     4261 catcgctgat gcgctcgatc gccttggcgc gatcctccgg gtcggcgatc ttgtcggcgt
     4321 acttgcgcgc cgtttccgca tcgcgcagcg ctttgtcggc cagcgcctcg gcgtttttcg
     4381 cgcggccatg ctgcgccgcc atcttggcgt acagggcatt ggccgtcgct tcgtcggcgg
     4441 cattgcgc
//
LOCUS       DAOQJH010000134         4438 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_11788_length_4438_cov_3.817933, whole genome shotgun sequence.
ACCESSION   DAOQJH010000134 DAOQJH010000000
VERSION     DAOQJH010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 4438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4438
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11788_length_4438_cov_3.817933"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1642)
                     /locus_tag="PLQ18_04305"
     CDS             complement(<1..1642)
                     /locus_tag="PLQ18_04305"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNE00908.1"
                     /translation="MTDSGQGNTAQGQAAMQEQRLRQIVDQATDTFIVIDAAGCIADV
                     NAHGCQNLGYAREQLIGRPYALLDPLQPPARLAELTTALATGPLTIETHHRRSDGSEL
                     PVEVRFGALATGDGPCLLAICRDISERRALEAQLRFQSTHDLLTGLPNRNLLEDRLNM
                     AVAHARRRSRLVGVLCLDLIRFKQLVDTLGHTATDELLRQIARRLIGVVRPGDTVSRQ
                     AGDTFVIVLNELVAEERASQIAGRLREAVTTPLTVAGQEVYPDASIGIAVFPRDGSDC
                     DTLLRHAAAAMQRARQHPRNEPQFYAAAMNTWATERLGLEGKLRRALDGRELLLHYQP
                     QIELSTGHIVGTEALLRWRHPELGMVSPAEFIPLAEETGLILPIGDWVLRTACAQARA
                     WRDAGLPELRMCVNLSALQFAQPDLAERVAAVLAETGLPATALELEITESVLMRDVEA
                     AAAMIGTLNDMGVQLALDDFGTGYSSLAYLKRFRIQRLKIDRSFVRDITTDPDDAAIT
                     LAVIALAHSLRKSVIAEGVETEEQRHFLSERGCDEVQGF"
     gene            complement(1732..2490)
                     /locus_tag="PLQ18_04310"
     CDS             complement(1732..2490)
                     /locus_tag="PLQ18_04310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE00909.1"
                     /translation="MHADTAALRRHVLARAPLCAPGDGPVLGLVFVTLFGALALDAAF
                     GMTGQVLGSLAVWALLLVLLARMTPAWRRTLVIATAFALAAELFFSLVLGWYDYQFLN
                     VPAFVPPGHVLLFLLGPALACRLPQWLVIAVPLAAGAWALWASLTGIGPFDGLLCAVF
                     LLAYRFGRSRPTYALMLLLALALELWGTGLGNWRWQPVVPGLGLATVNPPLLAGTFYA
                     LFDMYVMLSARACHRHFGDLPGAAQPGERSRMPP"
     gene            2703..3737
                     /locus_tag="PLQ18_04315"
     CDS             2703..3737
                     /locus_tag="PLQ18_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018717614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-6-deoxy-delta-3,4-glucoseen reductase"
                     /protein_id="HNE00910.1"
                     /translation="MGFQVSLQPGGSTFVVEAGEAVLDAALRAGCHIPYGCRGGSCGA
                     CAGRLLSGDYHYPQGLPPGLSPREAEAGRVLLCQARARSDLVFELHELRMPPGIEVRT
                     LPARVVSMQRLADDVMRLYLKLPASENFRYLAGQYVDILLRGGGRRGFSIANRPGGED
                     FLELHVRLVPGGEFTTHVFAEMKERAILRLQGPLGTFFVREDSSRPLLMMAGGTGFAP
                     LKAMIEQIYASGPHTRPVHLYWGARHRAGLYLHELVEGWIDARPGLRYTPVLSEPLPE
                     DDWRGRTGWVHEALAADYPDLSGFDVYMSGPPAMVRAARERFAQQGLPDGQLCFDAFE
                     FAHTTSGNGE"
     gene            complement(3760..4362)
                     /locus_tag="PLQ18_04320"
     CDS             complement(3760..4362)
                     /locus_tag="PLQ18_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010106716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE00911.1"
                     /translation="MIPVAAQPEPADFDRRVRQPGLAFLAGQGITHSAPSGLRWKDHW
                     RHCLVHLHAAYDGICAFLGVYIEPVTGARTVEHFRPKRTHPQLAYEWSNYRLVCNTLN
                     GRKGDHEDVLDPFTLAPDTFCIEFTSGRIYPDPVLEAREPVTFAAAEATIRRLRLDDA
                     ECRGVRTAHFGDYLAGDVSADFLRRHSPFVWYELRRQGLL"
BASE COUNT          648 a         1500 c         1656 g          634 t
ORIGIN      
        1 agaagccctg cacctcgtcg cagccgcgct cgctgaggaa gtggcgctgt tcctcggtct
       61 cgacgccttc ggcgatcacc gacttgcgca ggctgtgcgc gagggcgatc accgccagcg
      121 tgatcgcggc gtcgtcgggg tcggtggtga tgtcgcgcac gaagctgcgg tcgatcttca
      181 gccgctggat gcggaagcgc ttgaggtagg cgagcgagga atagccggtg ccgaagtcat
      241 cgagcgcgag ctgcacgccc atgtcgttca gcgtgccgat catcgccgcg gcggcctcga
      301 catcgcgcat cagcaccgat tcggtgatct ccagctccag cgccgtcgcc ggcaggccgg
      361 tttcggcgag cacggcggcg acgcgctcgg ccagatcggg ctgcgcgaac tgcagcgccg
      421 acaggttcac gcacatgcgc agctccggca ggccggcgtc gcgccaggcg cgggcctgcg
      481 cgcaggcggt gcgcagcacc cagtcgccga tcggcaggat cagcccggtc tcctcggcga
      541 gcgggatgaa ctccgccggc gagaccatgc cgagctccgg atgcctccag cgcagcagcg
      601 cctcggtgcc gacgatgtgc ccggtgctca gctcgatctg cggctggtag tgcagcagca
      661 gctcgcggcc gtcgagcgcg cggcgcagct tgccttcgag cccgaggcgc tcggtcgccc
      721 aggtgttcat cgccgccgcg tagaactgcg gctcgttgcg cgggtgctgg cgggcgcgct
      781 gcatcgcggc ggcggcgtgg cgcagcagcg tgtcgcagtc gctgccgtcg cgcgggaaca
      841 cggcgatgcc gatgctggcg tccgggtaga cctcctgacc ggcgacggtc agcggcgtgg
      901 tcacggcctc gcgcaggcgt ccggcgatct ggctggcgcg ctcctcggcg acgagttcgt
      961 tgagcacgat cacgaaggtg tcgccggcct gacgcgagac ggtgtcgccg gggcgcacga
     1021 cgccgatcag gcgccgggcg atctggcgca ggagctcgtc ggtggccgtg tggccgagcg
     1081 tgtcgaccag ctgcttgaag cggatcaggt cgagacacag cacgccgacc agccggctgc
     1141 ggcggcgggc gtgcgcgacc gccatgttca ggcggtcttc gagcaggttg cggttcggca
     1201 ggccggtcag caggtcgtgc gtgctctgga agcgcagctg cgcttcgagc gcgcgccgct
     1261 cggagatgtc gcggcagatc gcgagcaggc acgggccgtc gccggtggcg agcgcgccga
     1321 agcgcacctc gaccggcagt tcgctgccgt cgctgcggcg gtggtgggtc tcgatggtca
     1381 gcggcccggt cgccagcgcg gtagtgagct cggcgagccg ggcagggggc tgcagcgggt
     1441 cgagcagcgc atacgggcgg ccgatcagct gctcgcgcgc gtagccgagg ttctggcagc
     1501 catgggcgtt gacgtcggcg atgcagccgg cggcgtcgat gacgatgaag gtgtcggtgg
     1561 cctggtcgac gatctgccgc agccgctgct cctgcattgc cgcctggccc tgcgccgtgt
     1621 tgccctgtcc ggaatccgtc acggtgtcct cgcatgcgga gccatgcacc gggcgtaccg
     1681 gcgcggcctc cagaggcatg agggtagcac cgcgagccgc cggaccggtc actatggcgg
     1741 catgcgtgac cgctcgcccg gctgcgctgc gcccggcaga tcgccgaagt gccggtggca
     1801 ggcacgcgca ctcagcatca cgtacatgtc gaacagcgcg tagaaggtgc cggcgagcag
     1861 cggcggattg accgtcgcca gcccgaggcc cggcaccacc ggctgccagc gccagttgcc
     1921 gaggccggtg ccccagagct ccagcgccag cgccagcagc agcatcagcg cataggtcgg
     1981 ccgcgagcgc ccgaagcggt aggcgagcag gaagaccgcg cacagcaggc cgtcgaacgg
     2041 cccgatgccg gtgaggctcg cccacagcgc ccaggcgccg gcggccagcg gcacggcgat
     2101 caccagccac tgcggcagcc ggcacgccag cgccggaccg agcaggaaca gcagcacgtg
     2161 gccgggcggc acgaaggccg gcacgttcag gaactggtag tcgtaccagc cgagcaccag
     2221 cgagaagaac agctccgcgg ccagtgcgaa ggccgtggcg atcaccagcg tccgccgcca
     2281 ggccggcgtc atgcgcgcga gcagcacgag cagcagcgcc cacaccgcga ggctgccgag
     2341 cacctgcccg gtcatgccga atgcggcatc gagggccagc gccccgaaca gcgtcacgaa
     2401 caccagcccg agcacggggc cgtccccggg ggcgcacagc ggggcgcggg cgaggacgtg
     2461 gcggcgcagg gcggcggtat cggcgtgcat ggagagcgga tgggggcggg cggcaggagg
     2521 tgtcagcctg cgttgccagc gggtttgcgg caagccgcac gtacgccggc gctctgcatc
     2581 cggatttgcg ctggcgcaag gtcacggcct gctgcaaggt gcagcatggc cgccatggtg
     2641 cggttccgca ccggcgccgg atcggcgaag atgcgcggcc tttggtgagg cgggggtcgg
     2701 ggatggggtt tcaggtcagt ctgcagccgg gcggcagcac gtttgtggtc gaggccggcg
     2761 aagcggtgct cgacgctgcg ctgcgtgccg gttgtcacat cccgtacggc tgccgcggcg
     2821 gcagctgcgg cgcctgcgcc gggcggctgc tgtccggcga ttaccactat ccgcagggcc
     2881 tgccgccggg gctgtcgccg cgcgaggccg aggccgggcg cgtgctgctc tgtcaggcgc
     2941 gggcgcgcag cgatctcgtg ttcgagctgc acgaactgcg catgccgccc ggcatcgagg
     3001 tgcgcacgct gccggcgcgc gtggtgtcga tgcagcgcct cgccgacgac gtgatgcgcc
     3061 tgtacctgaa gctgccggcc agcgagaact tccgctatct ggccggccag tacgtcgaca
     3121 tcctgctgcg cggcggcggc cggcgcggct tctcgatcgc caaccgcccg ggcggtgagg
     3181 atttcctcga actgcacgtg cgcctggtgc cgggcggcga gttcacgacc cacgtgttcg
     3241 ccgagatgaa ggagcgcgcg atcctgcgcc tgcaggggcc gctcggcacc ttcttcgtgc
     3301 gcgaggactc gtcgcggcca ctgctgatga tggccggcgg caccggcttc gcgccgctga
     3361 aggcgatgat cgagcagatc tacgccagcg gaccgcacac gcggccggtg cacctgtact
     3421 ggggcgcacg ccaccgcgcc gggctgtacc tgcacgaact ggtcgaaggc tggatcgacg
     3481 cccggccggg cctgcgctac acgccggtgc tgtccgagcc gctgcccgaa gacgactggc
     3541 gcggacgcac cggctgggtg cacgaggcgc tggccgccga ttacccggac ctctcgggct
     3601 tcgatgtcta catgagcggc ccgccggcga tggtgcgcgc cgcgcgcgag cgcttcgcgc
     3661 agcagggcct gcccgacggg cagctctgct tcgacgcctt cgagttcgcg cacaccacga
     3721 gcggcaacgg cgagtgagca gcacagcggc ggcggtgggt tacagcaagc cctgcctgcg
     3781 cagttcgtac cagacgaacg ggctgtgcct gcgcaggaaa tcggcgctta catcccctgc
     3841 gagatagtcg ccgaaatgcg cggtgcgcac accacgacat tcggcgtcgt cgagtcgcag
     3901 gcgtctgatg gttgcctcag cggcagcgaa cgtcaccggc tcccgcgcct ccagcaccgg
     3961 atccggatag atgcggccgc tcgtgaattc gatgcagaag gtatccggtg cgagcgtgaa
     4021 tggatcaagc acatcctcgt gatcgccttt gcggccattc agcgtgttgc agaccagccg
     4081 gtagttgctc cattcgtagg cgagttgcgg gtgcgttcgc ttcggccgga aatgttcgac
     4141 cgtgcgtgcc cccgtgaccg gctcgatgta gacgccgagg aaggcgcaga tgccgtcgta
     4201 ggcggcgtgc aggtgcacaa ggcaatggcg ccagtggtct ttccacctga gcccgctcgg
     4261 ggcgctgtgg gtgatgcctt gtccagccag gaaggcgagg ccgggctggc gcacgcgtct
     4321 gtcaaagtcc gccggctccg gctgtgcggc caccggaatc acagccagcc ctgtcgttcg
     4381 ccgacgtagc gccagcgcag ccagaacgga tcggtgtcgc cgagcacctg ccgcaacc
//
LOCUS       DAOQJH010000135         4422 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_11848_length_4422_cov_3.725670, whole genome shotgun sequence.
ACCESSION   DAOQJH010000135 DAOQJH010000000
VERSION     DAOQJH010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 4422)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4422)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4422
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11848_length_4422_cov_3.725670"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..444
                     /locus_tag="PLQ18_04325"
     CDS             <1..444
                     /locus_tag="PLQ18_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018718595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNE00912.1"
                     /translation="GRARAQCPTMLDYAVFAKHGSMYNTPPTFGWYLAGLIFEWLLAQ
                     GGLPAMAEKNARKAGRLYAYLDGSGFYRNPVDPACRSWMNVPFRLADESLDAVFLKEA
                     EAAGLVGLAGHRIVGGMRASLYNAMPEAGVEALIAFLQDFVQRRG"
     gene            471..1559
                     /gene="pheA"
                     /locus_tag="PLQ18_04330"
     CDS             471..1559
                     /gene="pheA"
                     /locus_tag="PLQ18_04330"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018954050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HNE00913.1"
                     /translation="MTEQEQLLAIRDRIDTIDRQILDLISARARCAQEVAQVKQAHGH
                     GGGNFYRPEREAQVLRRIMELNPGPLSSEEVGRLFREIMSACLVLERPLRIAFLGPEG
                     TFTQAAALKHFGHSALTLPQRAIDEVFREVESGDADYGVVPVENSTEGVVNHTLDMFM
                     QSSLNICGEVQLRIHHHLAGFADDPKKLTRIYSHAQSLAQCREWLDANVPGVPRLPVA
                     SNAEAARMARNEPGAGCITGQGALDIYELPVIVANIEDQPDNTTRFLIIGTQPIPPSG
                     DDRTTLLVSSYSRPGSLMALLQPLADHGLSMTRIESRPSRKSMWDYVFFIDFDGHIES
                     PEVVSALADLQQAASLFRVLGSYPKGVL"
     gene            1583..2716
                     /gene="hisC"
                     /locus_tag="PLQ18_04335"
     CDS             1583..2716
                     /gene="hisC"
                     /locus_tag="PLQ18_04335"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017838829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HNE00914.1"
                     /translation="MPLPCDFTALAAPGVRGLTPYQPGKPESELKREYGLDSVVKLAS
                     NENPLGPSPKALEAVYACLPELARYPDGNGFELKQALSARLAVDAARITLGNGSNDVL
                     ELLARAFLTPAHNAVMSAHAFAVYPLATLAVGAKLRTAPALAADSAMPYGHDLAAMRA
                     LVDDDTRLVFIANPNNPTGTWLAPAELEAFIASLPAQVLCVLDEAYGEYMSPGQLPDS
                     VGWLERYPNLVITRTFSKAYGLAGLRVGYALAHAQVTDLLNRVRQPFNVNLPALTAAT
                     AALGDAAYLAEAVALNRSGLQQWQDGLTALGLGWLPSAGNFLCVDLKGRSGRELFQRL
                     LPKGVILRPVDNYGLPGHIRVTLGTGRENAIALAALGEVLAAG"
     gene            2776..3636
                     /locus_tag="PLQ18_04340"
     CDS             2776..3636
                     /locus_tag="PLQ18_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HNE00915.1"
                     /translation="MIPRLAILGVGLIGGSLARALKRAQRVGEIVGCGRNEDQLRKAV
                     ELGVIDRYTTDPAEAVRGADVVVLGTPVSAMRAVLASIRDHLAPQAVLTDVGSAKGCV
                     VEDARAVFGGLPERFVPGHPIAGTEKSGVEASFAELFEHRRVILTPHVENAAPAVDLV
                     RTMWLATGAQVVEMGIRHHDEVLAATSHLPHVLAYTVVDTLAAMDDRAEIFRYAAGGF
                     RDFTRISASDPAMWRDICLANRECLVAVIDEYVGNLQRLRAALAAGDEPTVYETFRRA
                     KHARDNFYRE"
     gene            3651..3998
                     /locus_tag="PLQ18_04345"
     CDS             3651..3998
                     /locus_tag="PLQ18_04345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE00916.1"
                     /translation="MRAGHPFRAPRCAGVAIPSLPIRFPPPGLLAAVGLLLALLITAP
                     ASARMQCDCGNYLMSCMAGCLGNPYCTSRCQQFHDTCVSSCSGTYSGGDRHHRHDEDE
                     DHEEDRYPRHRHR"
     gene            complement(4123..4422)
                     /locus_tag="PLQ18_04350"
                     /pseudo
     CDS             complement(4123..4422)
                     /locus_tag="PLQ18_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017947259.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
BASE COUNT          665 a         1630 c         1456 g          671 t
ORIGIN      
        1 ggccgcgccc gcgcgcagtg cccgacgatg ctcgactatg cggtgttcgc gaagcacggc
       61 tcgatgtaca acacgccgcc gaccttcggc tggtatctcg ccggactgat cttcgaatgg
      121 ctgctcgcgc agggcggcct gccggcgatg gccgaaaaga atgcccgcaa ggccggcagg
      181 ctctacgcct acctcgatgg ctccggcttc taccgcaacc cggtcgatcc ggcctgccgc
      241 tcgtggatga acgtgccgtt ccggctcgcc gacgagtcgc tcgatgccgt gttcctgaag
      301 gaagccgagg ccgccggact ggtcgggctg gcagggcatc gcatcgtcgg cggcatgcgc
      361 gcgagcctct acaacgccat gcccgaggcc ggcgtcgagg cgctgatcgc gttcctgcag
      421 gacttcgtgc agcgccgggg ctgaggcccg gcatcaccgg tacccgaacg atgaccgaac
      481 aggaacagct gctggcgatc cgtgaccgca tcgacacgat cgaccgccag atcctcgatc
      541 tgatcagtgc ccgcgcgcgc tgcgcgcagg aagtcgccca ggtcaagcag gcgcacggtc
      601 acggcggcgg caatttctat cgccccgagc gcgaggcgca ggtgctgcgc cggatcatgg
      661 agctgaaccc cggcccgctg tcgagcgagg aggtcggccg gctgttccgc gagatcatgt
      721 ccgcgtgcct ggtgctggag cggccgctgc gtatcgcctt cctcggcccc gagggcacct
      781 tcacgcaggc ggcggcactc aagcacttcg gccacagcgc gctgaccctg ccgcagcgcg
      841 cgatcgacga ggtgttccgc gaggtcgaat ccggcgatgc cgactacggc gtcgtgccgg
      901 tcgagaactc gaccgagggc gtcgtcaacc acacgctcga catgttcatg cagtcctcgc
      961 tgaacatctg cggcgaggtg cagctgcgca tccatcatca tctggccggc ttcgccgacg
     1021 atccgaagaa gctcacgcgc atctactcgc atgcgcagtc gctcgcgcag tgccgcgaat
     1081 ggctcgatgc caacgtgccc ggcgtgccgc gcctgccggt ggcctccaac gccgaggccg
     1141 cgcgcatggc gcgcaacgaa cccggcgccg gctgcatcac cggccagggc gcgctcgaca
     1201 tctacgagct gccggtgatc gtcgccaaca tcgaggacca gccggacaac accacgcgct
     1261 tcctgatcat cggcacgcag ccgatcccgc cgtccggcga tgaccgcacg acgctgctgg
     1321 tgtcctcgta cagccggccc ggctcgctga tggcgctgct gcagccgctc gccgaccacg
     1381 gcctgtcgat gacgcgcatc gagtcgcgcc cctcgcgcaa gagcatgtgg gactacgtgt
     1441 tcttcatcga cttcgacggc cacatcgagt cgccggaggt cgtcagcgcg ctcgccgacc
     1501 tgcagcaggc ggcgagcctg ttccgcgtgc tcggttccta cccgaaaggc gtgctgtaaa
     1561 accccggatg ccggagaccc cgatgccgct accctgcgat ttcaccgccc tggccgcccc
     1621 cggcgtgcgc ggcctgaccc cgtaccagcc cggcaagccc gaaagcgagc tgaagcgcga
     1681 gtacgggctg gattcagtcg tcaagctggc ctcgaacgag aacccgctcg gcccgagccc
     1741 gaaggcgctc gaagccgtgt atgcctgcct gccggaactc gcgcgctacc ccgacggcaa
     1801 cggcttcgag ctgaagcagg cgctgtcggc gcgcctcgcg gtcgatgcgg cgcgcatcac
     1861 gctcggcaac ggctcgaacg acgtgctcga actgctggcg cgtgccttcc tgacgccggc
     1921 gcacaacgcc gtgatgtcgg cgcatgcgtt cgcggtgtat ccgctggcga cgctcgcggt
     1981 cggtgcaaag ctcaggaccg ccccggcgct cgccgccgac agcgcgatgc cctacggcca
     2041 tgatctggcc gcgatgcgcg ccctcgtcga tgacgacacg cgcctggtgt tcatcgccaa
     2101 cccgaacaac ccgaccggca cctggctcgc gccggccgaa ctcgaagcct tcatcgccag
     2161 cctgccggcg caggtgctct gcgtgctcga cgaggcctat ggcgagtaca tgagccccgg
     2221 gcagctgccg gactcggtcg gctggctgga gcgctacccg aacctcgtca tcacgcgcac
     2281 cttctcgaag gcttacggcc tcgccggcct gcgcgtcggc tacgcgctcg cgcatgcgca
     2341 ggtcaccgac ctgctgaacc gcgtgcgcca gcccttcaac gtcaacctgc cggcgctgac
     2401 ggcggcgacg gccgcgctcg gcgacgccgc ctacctcgcc gaggccgtgg cgctgaaccg
     2461 cagcggtctg cagcagtggc aggacggact cacggcgctc ggcctcggct ggctgccctc
     2521 ggccggcaac ttcctgtgcg tcgacctcaa gggccgcagc ggccgcgagc tgttccagcg
     2581 cctgctgccg aagggcgtga tcctgcgacc ggtcgacaac tacggcctgc ccggccatat
     2641 ccgcgtcacg ctcggcaccg gacgcgagaa cgccatcgcc ctcgccgcgc tcggcgaggt
     2701 gctggcggcg ggctgaagcc gccgcccgat gccactgtgc catcgtcagg gagattgccc
     2761 gtgagccacg acatcctgat cccgcgcctc gccatcctcg gcgtcggcct catcggcggt
     2821 tcgctggcgc gggcgctgaa gcgcgcgcag cgcgtcggcg agatcgtcgg ctgcgggcgc
     2881 aacgaggatc agctcaggaa ggccgtcgag ctcggcgtca tcgaccgcta cacgaccgac
     2941 ccggccgagg ccgtgcgcgg tgccgacgtg gtcgtgctcg gcacgccggt gtcggcgatg
     3001 cgggcggtgc tcgcatcgat ccgcgatcat ctggcgccgc aggccgtgct gaccgatgtc
     3061 ggcagcgcca agggctgcgt ggtcgaggac gcccgcgccg tgttcggcgg cctgcccgaa
     3121 cgcttcgtgc ccggacaccc gatcgccggc accgagaaga gcggcgtcga agcctcgttc
     3181 gcggaactgt tcgaacaccg gcgcgtgatc ctgaccccgc atgtcgagaa cgccgcgccg
     3241 gctgtcgacc tggtgcgcac gatgtggctc gccaccggcg cgcaggtcgt cgagatgggc
     3301 atccgccacc acgacgaggt gctggccgcg acctcgcacc tgccgcacgt gctcgcctat
     3361 accgtcgtcg acacgctggc ggcgatggat gatcgcgccg agatcttccg ctacgccgcc
     3421 gggggtttcc gcgacttcac gcgcatctcg gcctcggacc cggcgatgtg gcgcgacatc
     3481 tgtctcgcca accgcgaatg cctggtcgcg gtcatcgacg aatacgtcgg caacctgcag
     3541 cgcctgcgtg cggcgctcgc tgccggcgac gagccgaccg tctacgagac cttccgccgc
     3601 gccaagcacg cgcgcgacaa cttctaccgt gaatgagccc cggcaaggga atgcgtgccg
     3661 ggcatccgtt ccgtgcgccc cgctgcgccg gagtcgccat cccgagcctg ccgatccgtt
     3721 tcccgccccc ggggctgctg gccgccgtcg gtctgctgct ggcgctgctg atcacggcac
     3781 cggccagcgc ccggatgcag tgcgactgcg gcaattacct gatgagctgc atggccggct
     3841 gtctcggcaa cccgtactgc accagccgct gccagcagtt ccatgacacc tgcgtgtcga
     3901 gctgctccgg gacctactcc ggaggcgacc gccatcaccg gcatgacgag gatgaggatc
     3961 acgaagagga ccggtatccg cgccatcgcc accggtaaga cattgtttgc gcatcggtaa
     4021 aaccctgtgc cgacgggtgc cggtgcatcc aggtgttaca ccctgcgctg gtcgcggatg
     4081 ccgggctggc cgatgcttgc cccatccgca atccagccga caaccgccgc catgcgcatg
     4141 gccctccacc gcagcgatca ccggcttcga caggagcatc cgcgtcggtc ccatcggacc
     4201 gtcgtcggcc atgtcggtcg acacccggtt cccgcgcccc tcaccgatcc ccttgaggtc
     4261 cgccccggcg cagaaggtcc caccggttcc ggtcagcacc gcgacggatg cgtcctcgtc
     4321 cgcgtcgaag gcccggaagg cgtcgacgag catcgcggcg gtcgggccgt cgacggcgtt
     4381 gcggacctgc ggtcggtcga tggtcacgat cgtgaccggc cc
//
LOCUS       DAOQJH010000136         4416 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_11871_length_4416_cov_3.871818, whole genome shotgun sequence.
ACCESSION   DAOQJH010000136 DAOQJH010000000
VERSION     DAOQJH010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 4416)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4416)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4416
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11871_length_4416_cov_3.871818"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..178)
                     /locus_tag="PLQ18_04355"
     CDS             complement(<1..178)
                     /locus_tag="PLQ18_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNE00917.1"
                     /translation="MSPASSRPPLAEGTPLLAVEHVVKAYGPRKALDGVSLEVGRGEF
                     VALLGPNGAGKSTLF"
     gene            complement(175..930)
                     /locus_tag="PLQ18_04360"
     CDS             complement(175..930)
                     /locus_tag="PLQ18_04360"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012976003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNE00918.1"
                     /translation="MARVSGGGLSVELQAKYWPGSDVPVLAGLRLDVAAGEFLCLVGP
                     SGCGKSTLLAAIAGLDADFLGRIDAPPERGMLFQTPRLMPWLTVRDNLALVLADGRCS
                     PAAEALLDAMEIPPALLDRYPGQLSGGQQRRVALARAFAVRPRLLLLDEPFVSLDAPV
                     AQRLRELLLEHWARERPTVLFVTHDLDEALALGDRIVFLGGRPAGIVLELVLPEPRPR
                     APEAVWRLRRQLLSTHPELLSGRVIPDPETPRP"
     gene            complement(918..1685)
                     /locus_tag="PLQ18_04365"
     CDS             complement(918..1685)
                     /locus_tag="PLQ18_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNE00919.1"
                     /translation="MRTEPGLSRAAERRLRFAAFALLLLLWWGLALVLHSDVLPTPAA
                     VWERLWAHAVSGELWRHLGATLARVAASFLIAMLVGTAIGILLGGSRRLDVFFDGWLV
                     LGLNIPALVTIILCYVWFGLNDAAAVLAVAANKIPTVVVTVREGARAVDRELLAVATA
                     FRVPPLRRLTRVYLPQLLPYLFASARSGLSLIWKIVLVVELLGRPNGIGFRLNAFFQF
                     FDIAGILAYTLAFAAVVLAVEAWLLRPLERRLLGWRA"
     gene            complement(1675..2664)
                     /locus_tag="PLQ18_04370"
     CDS             complement(1675..2664)
                     /locus_tag="PLQ18_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNE00920.1"
                     /translation="MPRTVSSLLLRACLLFAGGLCAFTATALETVRVGFQQSGTVQWE
                     LQLMQELGLAEREGVRLELVPLATKDATHIALQGGAVDVVVDDWLWVSRARDEGRPYT
                     CVTHSLAAGALYARPDSGITRLADLAGKKLGVAGGAVDKSWLLLRAYAQKTLGVDLAQ
                     QVEPQYGAPPLLNQLALRGELPAVLNYWNYGARLSAAGFREVISMRTVLTGLGAPPGL
                     PVIAWVFPDEFAAQHGETLRGFLRASRAAKQALATDDAVWERLRPLMQAANDTEFRSL
                     RDTYRQGIPQHVGPQDALTASRIFALLARLGGEALVGRGTTLATGTFWTGYAD"
     gene            complement(2771..3388)
                     /locus_tag="PLQ18_04375"
     CDS             complement(2771..3388)
                     /locus_tag="PLQ18_04375"
                     /inference="COORDINATES: protein motif:HMM:TIGR03865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNE00921.1"
                     /translation="MDGSPVRFRDHAAVLCVPVLGLLCAAGPVMALQPPQMPELPLGT
                     LNPDGYRDHAWHAPVPATHPDATTVDTAEARALLESGAALAVDVMAAVERPAAAGEPG
                     AFIPNRPRRHIPGSIWLPNVGYDELTPAQARWYAQALARLSRGERDRGLLIYCIADCW
                     LSWNAVHRAASFGYTRLYWYPEGSDGWAEAGLALVEARPEPPPPP"
     gene            complement(3402..4361)
                     /locus_tag="PLQ18_04380"
     CDS             complement(3402..4361)
                     /locus_tag="PLQ18_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003201452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein relay system zinc metallohydrolase
                     1"
                     /protein_id="HNE00922.1"
                     /translation="MTARRKQGKQAGAVRAAALAFAVIVTVPLQAADTDYRLQPEPVA
                     PGTYLVAGVPENFSRSNGGNIANTAFVVTGAGVVVIDTGPSKRYGQQLRAAIARVTAE
                     PVVAVYLTHHHPDHALGNQAFADVPIRALPGTIEGLREQGNDFNSNLFRMVGDWMRGT
                     DPLLPAEAVAAGSVRIGTHEFELIPNAGHTAADLAILDRTTGTLFSGSVFNGRTPATP
                     NAVIADWIASLDRLAALNAERIVPAVGAITDNRAIAEDIDWLRWLDETLHEAAGRGAD
                     MAEVLRLPIPARFNDFALLREEYERSVSHLYPAIERASLGVRD"
BASE COUNT          657 a         1570 c         1654 g          535 t
ORIGIN      
        1 ggaacagcgt cgacttgccg gcgccgttcg gaccgagcag cgcgacgaac tcgccgcggc
       61 cgacttcgag gctgacgccg tcgagcgcct tgcgcggccc gtaggccttg acgacgtgct
      121 cgaccgcgag cagcggcgtg ccttcggcga gcggcggccg ggaggatgcg gggctcatgg
      181 gcgcggggtc tccggatcgg gtatgacgcg accggacagc agctccgggt gcgtcgaaag
      241 cagttgccgg cgcagccgcc acacggcttc gggcgcccgc ggtcgcggct cgggcaggac
      301 gagttccagc acgatgccgg ccggccggcc gccgaggaag acgatgcggt cgccgagcgc
      361 cagcgcctca tcgagatcgt gggtcacgaa cagcacggtc gggcgctcgc gcgcccagtg
      421 ttcgagcagc agctcgcgca gacgctgcgc gaccggtgca tcgagcgaca cgaagggctc
      481 atcgagcagc agcaggcgcg ggcgcacggc gaaggcgcgc gcgagcgcca cgcgccgctg
      541 ctgaccgccg gaaagctggc ccgggtagcg atccagcagc gccggcggaa tctccatcgc
      601 atcgagcagc gcctcggccg ccggcgagca gcggccgtcc gccagcacca gcgcgaggtt
      661 gtcgcgcacc gtcagccacg gcatcagccg cggcgtctgg aacagcatgc cgcgctcggg
      721 cggcgcgtcg atgcggccga ggaagtcggc atcgagtccg gcgatcgccg cgagcagcgt
      781 gctcttgccg cagccgctcg gcccgaccag acacaggaac tcgccggcgg ccacgtcgag
      841 ccggagaccg gcgagcaccg gcacgtcgct gcccggccag tacttggcct gcagctcgac
      901 cgacagcccg ccgccactca cgcgcgccac ccgagcagac gccgctccag cggccgcagc
      961 agccaggcct cgaccgcgag caccacggcc gcgaaggcca gcgtgtaggc aaggatgccg
     1021 gcgatgtcga agaactggaa aaaggcgttg agccggaagc cgatgccgtt cggccgcccg
     1081 agcagctcga ccaccagcac gatcttccag atcagcgaca gccctgagcg cgccgacgcg
     1141 aacagatacg gcagcagctg cggcaggtag acgcgcgtca gccggcgcag cggcggcacg
     1201 cgaaacgccg tcgcgaccgc gagcagctcg cgatcgacgg cgcgcgcgcc ttcgcgcacg
     1261 gtgacgacga cggtcggaat cttgttggcg gcgaccgcga gcacggcggc cgcgtcgttc
     1321 aggccgaacc agacgtagca gaggatgatc gtcaccagcg ccgggatgtt cagcccgagc
     1381 accagccagc cgtcgaagaa cacgtcgagc cggcgcgagc cgccgagcag gatgccgatc
     1441 gcggtgccga ccagcatcgc gatcaggaag ctcgccgcga cccgcgccag cgtcgcgccg
     1501 aggtggcgcc acagctcgcc gctgacggca tgcgcccaca gccgctccca gaccgccgcc
     1561 ggtgtcggca gcacgtcgga atgcagcacg agcgcgagcc cccaccacag cagcagcagc
     1621 agcgcgaagg cggcgaagcg cagccggcgt tcggcggcgc ggctcaggcc gggttcagtc
     1681 cgcatacccc gtccagaacg tgccggtggc gagcgtcgtg cccctgccga ccagcgcctc
     1741 gccgccgagg cgcgcgagca gcgcgaagat gcgggaggcc gtcagcgcat cctgcggccc
     1801 gacgtgctgc gggatgccct ggcggtaggt gtcgcgcagg ctgcggaatt cggtgtcgtt
     1861 cgccgcctgc atcagcggcc tgagcctttc ccagaccgca tcgtcggtcg cgagcgcctg
     1921 cttggcggcg cgcgaggcgc gcaggaagcc gcgcagcgtc tcgccgtgct gcgcagcgaa
     1981 ctcgtccggg aacacccagg cgatcaccgg caggccgggc ggcgcgccga ggccggtcag
     2041 caccgtgcgc atcgagatca cctcgcggaa accggcggcc gagaggcgcg cgccgtagtt
     2101 ccagtagttc agcaccgccg gcagctcgcc gcgcagcgcg agctggttca gcagcggcgg
     2161 tgcgccgtac tgcggctcga cctgctgcgc cagatcgacg ccgagcgtct tctgcgcgta
     2221 ggcgcgcagc agcagccagc tcttgtcgac cgccccgccg gcgacgccga gctttttgcc
     2281 ggcgagatcg gcgagcctgg tgatgccgct gtcgggccgc gcgtacagcg cgccggccgc
     2341 gagcgaatgc gtgacgcagg tgtagggccg gccctcgtcg cgggcacgcg acacccacag
     2401 ccagtcgtcg acgacgacat cgacggcacc gccctgcagc gcgatgtgcg tggcgtcctt
     2461 ggtggcgagc ggcaccagtt cgaggcgcac gccctcgcgt tcggcaaggc cgagttcctg
     2521 catcagctgc agctcccact gcacggtgcc ggattgctgg aagccgacgc gcacggtctc
     2581 gagcgcggtg gcggtgaacg cgcacaggcc gccggcgaac agcaggcagg cgcgcagcag
     2641 cagcgaggat acggttctgg gcatcgatcg atccgtcagg tcgggcggcg gcgcggtgcc
     2701 gtcgggcagg tccgggcgga gggtaagggc gaacgggaag ccgcggccac tgccgcgccg
     2761 gcatggcggc tcacggtggc ggtggcggtt ccggccgggc ctcgaccagc gccagcccgg
     2821 cctcggccca gccgtcgctg ccctcgggat accagtacag gcgcgtgtag ccgaagctgg
     2881 cggcgcgatg cacggcgttc cacgacagcc agcagtcggc gatgcagtag atcagcaggc
     2941 cgcggtcgcg ctccccgcgg ctcaggcgcg ccagcgcctg cgcgtaccag cgcgcctgcg
     3001 ccggcgtcag ctcgtcgtag ccgacgttcg gcagccagat cgagccgggg atgtgccggc
     3061 gcggccggtt cgggatgaag gcgccgggct cgccggctgc ggccggacgt tcgacggcgg
     3121 ccatcacgtc gaccgccagc gccgcgccgg attcgagcag cgcgcgcgcc tcggcggtgt
     3181 cgaccgtggt ggcgtccgga tgcgtggccg gcaccggcgc gtgccaggca tggtcgcggt
     3241 agccgtccgg gttcagcgtg ccgagcggca gctccggcat ctgcggcggc tgcagcgcca
     3301 tgaccgggcc ggcggcgcac agcaggccga gcaccggcac gcagagcacg gcggcatgat
     3361 cgcggaagcg caccggcgaa ccgtccaccg gggccggcac cctaatcgcg cacgccgagc
     3421 gaggcgcgct cgatcgccgg gtacaggtgc gagaccgagc gctcgtactc ctcgcgcagc
     3481 agcgcgaagt cattgaagcg cgccgggatc ggcaggcgca gcacctcggc catgtcggcg
     3541 ccgcgcccgg cggcctcgtg cagggtttca tccagccagc gcagccagtc gatgtcctcg
     3601 gcgatcgcgc ggttgtcggt gattgccccg accgccggca cgatgcgctc ggcgttcagc
     3661 gccgccagcc ggtcgaggct cgcgatccag tcggcgatca cggcgttcgg cgtcgccggc
     3721 gtgcggccgt tgaacacgct gcccgagaac agcgtgccgg tcgtgcgatc gaggatcgcg
     3781 agatcggcgg ccgtgtgccc ggcgttcggg atcagctcga actcgtgagt gccgatgcgc
     3841 acgctgccgg cggcgacggc ctcggccggc agcagcggat cggtgccgcg catccagtcg
     3901 ccgaccatgc ggaacaggtt cgaattgaag tcgttgccct gctcgcgcag gccctcgatc
     3961 gtgcccggca gggcacggat cggcacgtcg gcgaaggcct ggttgccgag cgcgtggtcc
     4021 gggtggtgat gggtcaggta gacggcgacg accggctcgg cggtgacgcg cgcgatcgcc
     4081 gcgcgcagct gctggccgta gcgcttcgac ggcccggtgt cgatgacgac gacgccggcg
     4141 ccggtgacga cgaaggcggt gttggcgatg ttgccgccgt tgctgcgcga gaagttctcg
     4201 ggcacgccgg cgaccagata cgtgccgggg gcgaccggct cgggctgcag ccggtagtcg
     4261 gtgtcggcgg cctgcagcgg cacggtgacg atgaccgcga acgcgagcgc cgccgcgcgc
     4321 accgctcctg cctgcttccc ctgcttcctt cgggcagtca aggcgcttct ccggcgatgc
     4381 gtccggctgt gttgtcgtgg ccggcgatgc gcaccg
//
LOCUS       DAOQJH010000137         4415 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_11882_length_4415_cov_4.316514, whole genome shotgun sequence.
ACCESSION   DAOQJH010000137 DAOQJH010000000
VERSION     DAOQJH010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 4415)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4415)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4415
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11882_length_4415_cov_4.316514"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /locus_tag="PLQ18_04385"
     CDS             complement(<1..239)
                     /locus_tag="PLQ18_04385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE00923.1"
                     /translation="MSASPEPRRPPLPRRPVRRAAPRGEARAGVLALVLCGALIAGVI
                     VPYVRDRLPAPPAASSASVQSFSAPPLAQRNLPAPA"
     gene            complement(808..2661)
                     /locus_tag="PLQ18_04390"
     CDS             complement(808..2661)
                     /locus_tag="PLQ18_04390"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNE00924.1"
                     /translation="MVPSLRQRSPSTADDGLATTVSNHIGLAYFEALVRRLAVALDAG
                     FACVARHEPETGRLLPVASHSTDDAHDWPAALDGAPWPLVLAGVTVLQADGADALYAG
                     NTALAGVRACAGTPLVDAGGRTLGLIAVLGRRPFAQPAMVALVLELFAERAAAELERL
                     AAERELQRTGAALRRQQLALLRIVHAELLVEAEADTALARIAEIAGETLDADRAEIWQ
                     ADAAFGQVRRCASWHHRHGRGHDHDHDHDHDGPALDTRDCPGFVGTLRAQMLLDGSDP
                     RHAQALAEWRALAPPDSEAGASVLVLGISSGRTLHGLLQLECRRTAAARVWGIDELGF
                     ARSVCDLLALTLVTRALRRTVAELEASQQRYRALYEDIDTLARERLQALETHQQRMHQ
                     MQRQFVYMASHELRTPLAIIDSAAQRILRRADNLSRTELVERIGRIRAAVSRMGYLVD
                     STLDVARFESGTVSYRPGPVDPAALLTRVCQQQQELAPHHRIACRFEALPPSMSGDAG
                     LLEQVFANLLSNAVKYSPDADEIEVHAFADATRLVVRVCDHGVGIPAQEMPRLFEKFF
                     RASTAVGIPGTGIGLNITRQIVDLHGGILTVDSHVGTGTTFTVKLPLPSAA"
     gene            complement(2820..3515)
                     /locus_tag="PLQ18_04395"
     CDS             complement(2820..3515)
                     /locus_tag="PLQ18_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006748188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNE00925.1"
                     /translation="MPPSPAPEALDRLLRQTYLFAALDEPRLAVVRHGMRVLHLHEGQ
                     RLFDVGQPAERFFLVASGQIKLFRVSLDGNEKVIEIAGPGKTFAEAVMFMQRRDYPVS
                     TAALTEARVCSFDNHTYLELLRASPSLCLSMLGEMSMRLHARLNEIDHLTLQNASFRV
                     VHYLIDQLIEHGDGSGSIDLSTPKNIIASRVSIKPETFSRILRTLADEGIVRIDGKHI
                     EVPDCRRLQQWGR"
     gene            3667..4113
                     /locus_tag="PLQ18_04400"
     CDS             3667..4113
                     /locus_tag="PLQ18_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011136257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HNE00926.1"
                     /translation="MNPLNELLEGQHRQADAALADAELQVAAGDWPAARAAGSALDDE
                     LRAHFRREEAVLFPAFEAATGSGCGPTRVMRDEHEQIRTLLDALRGAGERCDADAWLG
                     LSDTLVLLMQQHNMKEEQILFPMAARLLAARLPALLDGLRPAAVSA"
     gene            4135..>4415
                     /locus_tag="PLQ18_04405"
     CDS             4135..>4415
                     /locus_tag="PLQ18_04405"
                     /inference="COORDINATES: protein motif:HMM:NF021520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2249 domain-containing protein"
                     /protein_id="HNE00927.1"
                     /translation="MTTAEHLLDVGGLEPPEPLFATLDAVDALGPGEWLRLLHRREPF
                     PLYGHLLERDCAYRVLVSEPGRYEALIWRLDDPLASVACAGDDDGDGCA"
BASE COUNT          647 a         1446 c         1631 g          691 t
ORIGIN      
        1 gccggcgccg gcagattccg ctgtgcaagc ggcggggcgc tgaaggactg cacgctggcg
       61 ctgctcgcag ccggcggcgc cggcagccgg tcgcgcacgt acggaacgat cacaccggcg
      121 atcagcgcgc cgcagaggac cagcgccagt acgccggcac gagcctcgcc gcgtggtgcg
      181 gcgcggcgga caggacgacg gggcagcggg ggacggcgag gctcggggga cgcggacatg
      241 acacttccct gtggtgcgat gcatcagaag cgcagtctag tcggtgtacg gatgacgggc
      301 cagtgtagcg gccggtatcc actggcaggg cgggatcgtc agcatcccgg agcggtggag
      361 gatgcaagtg actgaatcaa aaaattttaa tgaggctcag ggccgcaagc cggcgtcatg
      421 cgccgacgga ggagcggccg gattgccagg cagggttgtc agcccggcct gacggttcag
      481 attttttcat gcgcgcatca ggccagatgg tttgtgcaat gcagcatcgg cgggcaaact
      541 ggcttcggca tcctacccgg atgtcgcttc tcctcctttg gtggttgttt tggcccgggc
      601 cgtcccgggc cttttttttg tccggtacaa aaaagttcag gccagcgtga atatccgtcg
      661 tttgccgcgt gtgccgcgcg tgccgacact attccccgac atccgcgtga tgtttctctt
      721 ctcctccttt ggtggttgct tgagcccggc ttcgatgccg ggcttttttt tggaggggcg
      781 cgcagccggg gaggaggacg gtccggctca ggccgcggac ggcagcggca gcttgaccgt
      841 gaaggtggtg ccggtgccga cgtggctgtc gaccgtcagg atgccgccgt gcaggtcgac
      901 gatctggcgc gtgatgttca ggccgatgcc ggtgccgggg atgccgaccg cggtgctggc
      961 gcggaagaac ttctcgaaca gccgcggcat ctcctgagcc gggatgccga cgccgtggtc
     1021 gcacacgcgc acgacgaggc gcgtggcatc ggcaaaggca tgcacctcga tctcgtcggc
     1081 atcgggcgag tacttgacgg cgttgctgag caggttggcg aacacctgtt cgagcaggcc
     1141 ggcgtcgccg ctcatgctgg gtggcagcgc ctcgaaccgg caggcgatgc ggtggtgcgg
     1201 ggcgagttcc tgctgctgct ggcagacgcg cgtcagcagc gccgcggggt cgaccggtcc
     1261 gggccggtaa ctgaccgtgc cgctttcgaa gcgcgcgaca tcgagcgtcg agtcgaccag
     1321 atagcccatg cgcgacacgg ccgcgcggat cctgccgatg cgctcgacca gttcggtccg
     1381 cgacaggttg tcggcgcggc gcaggatgcg ctgggcggcg ctgtcgatga tcgcgagcgg
     1441 cgtgcgcagc tcgtgcgagg ccatgtacac gaactggcgc tgcatctggt gcatgcgctg
     1501 ctggtgggtt tcgagcgcct gcaggcgctc gcgggccagc gtgtcgatgt cctcgtacag
     1561 cgcccggtag cgctgctgcg aggcttcgag ttcggcgacg gtccggcgca gcgcgcgggt
     1621 gaccagcgtc agcgccagca ggtcgcagac cgagcgcgcg aagccgagtt cgtcgatgcc
     1681 ccagacgcgg gcagccgcag tgcgccggca ttcgagctgc agcagcccgt gcagcgtccg
     1741 gccgctgctg atgccgagca ccagcaccga ggcgccggcc tcggaatccg gcggcgcgag
     1801 cgcccgccac tcggcgaggg cctgcgcgtg gcgcgggtcg ctgccatcga gcagcatctg
     1861 cgcgcgcagc gtgccgacga agcccgggca gtcgcgggta tcgagcgcag ggccgtcgtg
     1921 gtcgtggtcg tggtcatggt cgtggccgcg accgtgccgg tgatgccagg acgcgcaccg
     1981 gcgcacctgt ccgaaagccg catcggcctg ccagatttcg gcgcgatcgg catccagggt
     2041 ctcgccggcg atctcggcga tgcgtgcgag ggcggtgtcg gcctcggcct cgaccagcag
     2101 ctcggcatgc acgatgcgca gcagcgcgag ctgctggcgg cgcagcgccg ccccggtgcg
     2161 ctgcagttcg cgctcggccg ccagccgttc gagttcggcc gctgcccgct cggcgaacag
     2221 ctccagcacc agagcaacca tcgccggctg cgcgaacggc cgccggccga gcaccgcgat
     2281 cagaccgagc gtgcggccgc cggcatcgac gagcggcgtg ccggcgcagg cgcgcacgcc
     2341 ggcgagagcg gtgttgccgg catacagcgc atcggccccg tcggcctgca gcaccgtgac
     2401 cccggcgagc accagcggcc agggcgcacc gtcgagggcc gccggccagt cgtgcgcgtc
     2461 atcggtgctg tggcttgcga ccggaagcag acggccggtt tccggctcat ggcgggcgac
     2521 gcaggcgaag ccggcatcga gcgcgacggc gagccggcgc acgagggcct cgaagtaggc
     2581 gagaccgatg tggttggaaa ccgtcgtcgc gagaccgtcg tccgcggtgg acggcgatcg
     2641 ctggcgcaag ctgggaacca tgccggggct tctgctgggc gccgcgggtc ggcagcaggg
     2701 cggtcagggc gcttccgtca cccccggctg cgcccccggc cggcgcggac cccggggacg
     2761 aagcgcgcag catagcggtg ccggtggcgc ctgcccggtt aaggatggtg aagccggatt
     2821 cagcgccccc actgctgcag gcgacggcag tccggcacct cgatgtgctt gccgtcgatg
     2881 cgcacgatgc cttcgtcggc cagtgtgcgc aggatgcgcg agaaggtttc cggcttgatc
     2941 gatacgcgcg aggcgatgat gttcttcggc gtcgacagat cgatgctgcc gctgccgtcg
     3001 ccgtgctcga tcagctggtc gatcaggtag tgcacgacgc ggaagctcgc gttctgcagc
     3061 gtcaggtgat cgatctcgtt cagccgcgca tgcaggcgca tgctcatctc gccgagcatg
     3121 ctcaggcaga gcgacggcga ggcgcgcagc agttcgaggt aggtgtgatt gtcgaacgag
     3181 cagacccgcg cctcggtcag cgccgcggtg ctgaccggat aatcacggcg ctgcatgaac
     3241 atcaccgcct cggcgaaggt cttgccgggg ccggcgatct cgatgacctt ctcgttgccg
     3301 tccagcgaca cgcgaaacag cttgatctgg ccgctggcga ccaggaagaa gcgctcggcc
     3361 ggctggccga catcgaacag gcgctggcct tcgtgcaggt gcagcacgcg catgccgtgg
     3421 cgtacgacgg cgagccgggg ctcgtccagg gccgcgaaca gataggtctg gcgcagcagg
     3481 cgatcgaggg cttcgggggc gggggatggt ggcatccgcg catgataggt cggccgccgg
     3541 cgggcgcacg actctcctgt ctgcataccc cgtccggtgg cccgatgtgg ttgatgcagg
     3601 ttaagggcgc acggacgatc cctctgcaga ctgcccggca ctccctccgt tgctgcgtgc
     3661 atgtccatga atccgctcaa tgaactgctc gaaggccagc accgccaggc ggatgcggcc
     3721 ctcgccgatg ccgaactgca ggtggccgcc ggcgactggc cggccgcccg tgccgccggc
     3781 agcgccctcg atgacgaact gcgcgcccac ttccggcgcg aggaagccgt gctgttcccg
     3841 gccttcgagg ccgccaccgg cagcggttgc ggtccgaccc gggtcatgcg cgatgagcac
     3901 gagcagatcc gcacgctgct cgatgcgctg cgcggcgccg gcgagcgctg cgatgccgat
     3961 gcctggctgg gcctgtccga cacgctggtg ctgctgatgc agcagcacaa catgaaggag
     4021 gaacagatac tgttcccgat ggccgcccgg ctgcttgcgg ctcgcctgcc ggcgctgctc
     4081 gatggcctgc gcccggcggc ggtcagcgcc tgactgcctg caggacgcac gacgatgacg
     4141 accgcggaac acctgctcga tgtcggtggc ctggagccgc cggaaccact gttcgcgacg
     4201 ctggatgccg tggatgcgct cggcccgggc gagtggctgc gcctgctgca ccggcgcgaa
     4261 cccttcccgc tgtacgggca tctgctcgaa cgcgattgcg cgtatcgcgt gctggtctcg
     4321 gaacccgggc gctacgaggc gctgatctgg cggctggacg atccgctcgc cagtgtcgcc
     4381 tgcgccggag atgacgacgg cgacggctgc gcatg
//
LOCUS       DAOQJH010000138         4413 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_11890_length_4413_cov_4.012391, whole genome shotgun sequence.
ACCESSION   DAOQJH010000138 DAOQJH010000000
VERSION     DAOQJH010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 4413)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4413)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4413
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11890_length_4413_cov_4.012391"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1977)
                     /locus_tag="PLQ18_04410"
     CDS             complement(<1..1977)
                     /locus_tag="PLQ18_04410"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNE00928.1"
                     /translation="MMRESEASRLLFLIRMLLPLLLLTLAVMLYQAYTDFRSTLLDRA
                     GQQMRIDAVAGASQFAELLREYRESLKWLAVRSARLDATDTPPDMLAALLDHYRFIHA
                     GTVVAIERRAADARLDAHAGLSLPPPAQDDTAAALLLQLAGRTVLVLTEPLTSAAGET
                     ARLRLALDLTTLASRALGALTKRDDVEGWLFDAEGRVLWQAHPAGAVPAAAASSPTAD
                     DFGVLRERLRRETAGSGLFRLQVWRDAQAHARDQLVGFAALPAEAGGLVLASAVEYRH
                     IAAPLDQFALQLLLIGGVLTLTLAATAALLQRFRASAGRRQQRLDRLQALIGRVDQAM
                     FDCEDPDRLHDSLARTLAEPVPGVQTALATRAADLADPLARERAQQVLSGGAARWDEN
                     RDGSVDALLPVSAPDGQPVAVLWFRQLGTPPPDAASRRLITRIAQDISHALVRMEDRS
                     RRNAAETDLRLVASVFENIREAAVITDADNRILRVNPAFTTITGYQPSEVIGQTPALL
                     ASGRHGREFYTAMWRSLQDTGSWSGEICNRRKDGCEYLEWLSISVVRDAAGVIMHHVA
                     VFADLSEHTAMRARIDWLASHDALTGLPNRASFIAALPGLLQRMAPGHCCAVLNLDLR
                     RFQRLNETLGRSGGDRVLQALAERLNALAARPACL"
     gene            2409..3131
                     /locus_tag="PLQ18_04415"
     CDS             2409..3131
                     /locus_tag="PLQ18_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004357550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HNE00929.1"
                     /translation="MSAADLMAAAQRLSAALAPLHFRAPVTHVYNPLDYAGLTYGQYL
                     QRWGDGRRRVLFLGMNPGPFGMVQTGVPFGEIHAVRDWLGIDAPIGRPAVEHPKRPVE
                     GWACRRSEVSGRRLWGLFAGRFGTPQAFFAGHFVLNWCPLAFLADGRNLTPDRLPSDE
                     FAPLAAACDRHLREACAALEPEWLIGVGRFARLRAQHALTGPAAPRIGEILHPSPASP
                     AANRGWAAAASAQLQELGVWGA"
     gene            3206..>4413
                     /locus_tag="PLQ18_04420"
     CDS             3206..>4413
                     /locus_tag="PLQ18_04420"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HNE00930.1"
                     /translation="MSQPMMSATSELPSNVVAEARLPAVLLAIVLGWAALIGALAYFT
                     ERDIAGDRAQTLETAAQRAETLLPLFGNSVQRALDDTRLLLSALELALESNGGDGAAR
                     DLLQRFGAPQDGMRQRISIADARGRLIASTADLPAASLSVADRDYFQRLAAPDAPGWI
                     LGKPVISHVTDDWTIPLAVRRVAADGRFTGALITAIDPQWLLTMAGSMDLGRTGTVGV
                     VDFDGTVYLRRDIDLHHAGAASTIGTTNPRLIVLEPARTAPSGRFSGTSALDGLRRLG
                     SYRVLPQYGLIVAYGLGEDEVLTQHMRRAGAERRLALLSGVLLTAIAGGLFLWRLRDW
                     RQARQLLAAWRQAHTAARTDVLTGLGNRRGYEEHIGALLASRPPMPSSLLYIDCDDFK
                     RINDSAGHEAG"
BASE COUNT          628 a         1542 c         1582 g          661 t
ORIGIN      
        1 caggcaggcc gggcgggcgg cgagggcatt caggcgctcg gccagcgcct gcagcacgcg
       61 gtccccgccg ctgcgcccga gcgtctcgtt caggcgctgg aagcggcgca ggtcgaggtt
      121 cagcaccgcg cagcaatgcc cgggcgccat gcgctgcagc agccccggca gcgcggcgat
      181 gaagctcgcg cgattcggca ggccggtcag cgcgtcatgg ctcgccagcc agtcgatgcg
      241 cgcgcgcatc gccgtgtgct cggacaggtc ggcgaacacc gcgacgtggt gcatgatcac
      301 gccggcggca tcgcgcacga cgctgatgct cagccattcc aggtattcgc agccgtcctt
      361 gcgccggttg cagatctcgc cgctccagct gccggtgtcc tgcagcgagc gccacatcgc
      421 cgtgtagaac tcgcggccgt ggcggccgga ggcgagcagc gccggcgtct gtccgatgac
      481 ctcggacggc tggtagccgg tgatggtcgt gaacgccgga ttgacgcgca ggatgcggtt
      541 gtcggcatcg gtgatcaccg ccgcctcgcg gatgttctcg aacacgctgg cgaccaggcg
      601 caggtcggtt tcggcggcgt tgcgccggct gcgatcctcc atgcgcacga gggcgtggct
      661 gatgtcctgc gcgatgcgcg tgatcaggcg gcgcgatgcc gcatcgggcg gcggagtccc
      721 gagctggcgg aaccacagca cggcgaccgg ctggccgtcc ggcgcggaca ccggcagcag
      781 cgcatcgacg ctgccgtcgc ggttttcgtc ccagcgcgcc gcgccgccgc tcagcacctg
      841 ctgcgcgcgt tcgcgcgcga gcggatcggc cagatcagcg gcccgggtcg caagcgcggt
      901 ctgcacgccc ggcaccggct cggccagcgt gcgggcgagg ctgtcgtgca gccggtccgg
      961 atcctcgcag tcgaacatgg cctgatcgac gcgtccgatc agcgcctgca ggcgatcgag
     1021 ccgctgctgg cggcgtccgg cgctggcgcg gaaacgctgc agcagcgcgg cggtcgccgc
     1081 cagcgtcagc gtcagcaccc cgccgatcag cagcagctgc agtgcgaact gatcgagcgg
     1141 ggcggcgatg tgccggtatt cgacggcact ggcgagcacg aggccgccgg cttcggccgg
     1201 cagcgccgcg aagccgacca gctggtcacg ggcatgcgcc tgcgcatcgc gccagacctg
     1261 cagccggaac aggccgctgc cggcggtttc ccggcgcagc cgttcgcgca gcacgccgaa
     1321 gtcatcggcg gtgggggagg aggccgctgc cgccggaacc gctccggccg ggtgcgcctg
     1381 ccagagcacc cgaccctcgg cgtcgaacag ccagccctcg acatcgtcgc gtttcgtcag
     1441 cgcccccagg gcgcggctgg ccagcgtcgt cagatcgagc gcgaggcgca gccgggcggt
     1501 ttcgccggct gcggacgtca gcggttcggt gagcaccagg acggtgcgcc cggcgagctg
     1561 cagcaacagc gccgcggcgg tgtcgtcctg cgccggcggc ggcagggaca ggccggcgtg
     1621 ggcgtccagc cgtgcatcgg cggcacgccg ttcgatggcg acgaccgtgc cggcgtggat
     1681 gaagcggtag tgatccagca gggccgcaag catgtccggc ggggtgtcgg tcgcatccag
     1741 ccgtgcggag cgcaccgcca gccatttcag cgactcgcga tactcgcgca gcagttcggc
     1801 gaactggctg gcgccggcga cggcatcgat gcgcatctgc tggccggccc gatcgagcag
     1861 cgtgctgcga aagtccgtgt aagcctgata cagcatcacc gccagcgtca gcagcagcag
     1921 gggcaacagc atccggatca ggaacagcag gcgggaggct tcgctctcac gcatcatcgc
     1981 cgcccgcacg tggcggagcg gcgccggcga cggcgctcgc gacggcggcc ggggcggggc
     2041 caggcagtcg cacgcagatc agggcagaag gaggggagtg cagcttgcgc tcgctcatcg
     2101 tggtgtcctc tttttgatca gcgcgatgga gcctgcaacg gtcgcggata cccgccggcg
     2161 accgcagccc ggaggctgca atcataatgc ggcaggtctg cgccgtagcg gtagatggac
     2221 aagcgcaagg ccggtttcat gggtatcgaa accatgttcc gctgctggac aactggcggt
     2281 aatgctggct ctggcaggga ttgcgctgtg catccgcgac cgctcctgct atgcaatgtc
     2341 aagcaaactt gacaacaatc gaggtcaatg gaaacaatgc ttaccttttc gatcccgtcg
     2401 gcggtccgat gagtgcagcc gatctgatgg cggcggcgca gcgcttgtcg gcggccctgg
     2461 cgccgctgca tttccgggcg cccgtgaccc acgtctacaa cccgctcgac tatgccggtt
     2521 tgacatatgg gcaatacctg caacgctggg gagacggtcg ccggcgcgtg ctgttcctcg
     2581 gcatgaatcc gggacccttc ggcatggtcc agaccggcgt gccgttcggt gaaatccacg
     2641 ccgtgcgcga ctggctgggc atcgacgccc cgatcggtcg ccccgccgtc gagcatccga
     2701 agcggccggt cgaaggctgg gcctgccggc gttcggaggt cagcggccgg cggctgtggg
     2761 gcttgttcgc cgggcgtttc ggcacgccgc aggccttctt cgccggacat ttcgtgctga
     2821 actggtgccc gctggccttt cttgccgacg gccgcaacct tacgcccgac aggctgccgt
     2881 ccgatgaatt cgcgccgctg gccgcggcct gcgaccggca cctgcgggaa gcctgtgccg
     2941 cgctcgaacc tgaatggctg atcggcgtcg gccgtttcgc gcgcctgcgc gcgcagcatg
     3001 cgctcaccgg gcctgctgcg ccgcgcatcg gcgagatcct gcacccgagc ccggccagcc
     3061 cggcggccaa tcgcggctgg gcggcggcgg cgagtgcaca gctgcaggaa ctcggggtct
     3121 ggggtgcctg atcaggcgtt gtcagcgttt cacgatgcgc agtgccatgg caggactatg
     3181 ctgcgctgac cgtgcatgcc ggtgaatgtc ccagccgatg atgagcgcca cctccgagct
     3241 gccgagcaat gtcgtcgcag aagcccgcct gccggcggtg ctgctggcga tcgtgctggg
     3301 ctgggcggcg ctgatcgggg cactcgcgta tttcaccgag cgcgacatcg ccggagaccg
     3361 ggcgcagacc ctggaaaccg ctgcgcagcg cgccgaaacc ctgctgccgc tgttcggcaa
     3421 cagcgtgcag cgcgcgctcg acgatacccg gctgctgctg tcggcgctgg aactggcgct
     3481 ggagtccaac ggcggcgatg gcgcggcccg cgacctgctg cagcgcttcg gcgcaccgca
     3541 ggacggcatg cgccagcgca tcagcatcgc cgatgcccgt ggccgcctga tcgcctcgac
     3601 cgccgacctg ccggccgcca gcctttcagt ggccgatcgc gattacttcc agcggctggc
     3661 ggccccggat gcgccgggct ggatcctcgg caagccggtc atcagtcatg tcaccgacga
     3721 ctggaccatt ccgcttgccg tgcgtcgcgt tgccgccgat ggccgtttta ccggcgcgct
     3781 gatcaccgcc atcgatccgc agtggctgct gacgatggcc ggctcgatgg atctcggccg
     3841 caccggcacg gtcggcgtcg tcgatttcga tggcacggtc tacctgcgcc gcgacatcga
     3901 cctgcaccat gccggggccg ccagcacgat cggcaccacc aatccgaggc tgatcgtgct
     3961 cgaaccggcg cgcaccgccc cgtccgggcg cttcagcggc acctcggcgc tcgacggcct
     4021 gcgccggctc ggctcctacc gcgtgctgcc gcagtacggg ctgatcgtcg cctacggtct
     4081 cggcgaggac gaggtgctga cccagcacat gcgccgcgcc ggtgccgagc gccggctggc
     4141 gctgctctcc ggcgtgctgc tgaccgcgat cgccggcggg ctgttcctgt ggcggctgcg
     4201 ggactggcgc caggcgcggc agctgctggc ggcctggcgc caggcgcaca ccgccgcgcg
     4261 caccgacgtg ctgaccggcc tcggcaaccg ccgcggctac gaggaacaca tcggggcgct
     4321 gctcgcgagc cgtccgccga tgccgtcgag cctgctgtac atcgactgcg acgacttcaa
     4381 gcgcatcaat gacagcgccg gccacgaggc cgg
//
LOCUS       DAOQJH010000139         4362 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_12088_length_4362_cov_4.150453, whole genome shotgun sequence.
ACCESSION   DAOQJH010000139 DAOQJH010000000
VERSION     DAOQJH010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 4362)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4362)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4362
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12088_length_4362_cov_4.150453"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..554
                     /gene="gidA"
                     /locus_tag="PLQ18_04425"
     CDS             <1..554
                     /gene="gidA"
                     /locus_tag="PLQ18_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007042518.1"
                     /note="GidA; glucose-inhibited cell division protein A;
                     involved in the 5-carboxymethylaminomethyl modification
                     (mnm(5)s(2)U) of the wobble uridine base in some tRNAs;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HNE00931.1"
                     /translation="ADLRLTETGRRLGLVGDVRWAAFEARREAIERETQRLRECWVRP
                     AALDPALAEAVLGGPLARETRALDLLRRPELDHAAVLRLAGLPESDLDADVIEQVEIQ
                     AGYAGYIERAQADIARARRQEDAAIPAGFDYAGVRGLSSEVREKLARVRPDTVGQAGR
                     IPGVTPAAVSLLLVHLKRLRAAG"
     gene            741..1019
                     /locus_tag="PLQ18_04430"
     CDS             741..1019
                     /locus_tag="PLQ18_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002684546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6489 family protein"
                     /protein_id="HNE00932.1"
                     /translation="MKLNVNVEATPQELREFLGLPNVQPLQQEMMEIIRENMLKGVQG
                     FDPVSLMKPFMAGAMPVQSIENLQKVFWDTFNKASTGQPSRTGTTGGS"
     gene            1100..1471
                     /locus_tag="PLQ18_04435"
     CDS             1100..1471
                     /locus_tag="PLQ18_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002691572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE00933.1"
                     /translation="MPKILVVDDEPNILLSLEFLMKHAGFEVRTATDGDAALAAVATE
                     VPDLILLDVMMPRRDGYEVCQAVRENPACARVKIIMLTAKGREIEREKGMALGADDYI
                     VKPFATQELLGRVRELLAGAT"
     gene            1510..3633
                     /locus_tag="PLQ18_04440"
     CDS             1510..3633
                     /locus_tag="PLQ18_04440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HNE00934.1"
                     /translation="MTVRIKFWALLLAPLAFTGLFAAVSLWKLPEIVIDPYMQENVRA
                     FYFAGGILACLAYAVVWAFIDAAFVRPLKALLHGARIISRSNPAHELMMPRNHLLGEV
                     PVIVQELGASLHQARKEIAAATATGTREIEAQKSRLEVVLRELSEGVLVCDADARVLL
                     CNPAARRLLPTPEALGLGRSIYGLLTRQPIEHTLDWLGFRQQQAGDGAPPATDAEFLC
                     SAADESRLFQCRMSLLPSSGAARRSFVLTLRDITSQVAPGTLGPSGFKGLVEDLRRQL
                     ASLRFAAESLSLYADMEPGQRRAFERVIFDESAALTERVEFLARHVRLTFAAQWPSAD
                     VLSTDVIGAALARLERSRGRMPVIVQSGDPLWLHCDGQSVMQLLEFVLTELANQEPAA
                     SFEIECLLGNRQVYVDIVWAGRPLPARLIQDWLERPLQHVAGAATVGEILRRHHSDLW
                     SQPHRKPGLAVLRIPLPSSPRQWSDEDGALPERPEFYDFDLELRPAQFGALADRPLKS
                     LDYVVFDTETTGLSPSRGDEMIQIAGLRVVQGRIREGETFDRLINPGKPIPKASIKFH
                     GITDAMVRDKPGAAVIVPQFRAFVGEPDTVLVAHNAAFDMKFLELKEAVTGVSFRQQP
                     VLDTLLLSGAIHDYTDDHTLDAIAERLGVDVHNRHTALGDTLVTAQVFVKLLDLLEAQ
                     GIVTLGQALALSEQMTAVRRQQAKF"
     gene            3635..3835
                     /locus_tag="PLQ18_04445"
     CDS             3635..3835
                     /locus_tag="PLQ18_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE00935.1"
                     /translation="MDAGERTARIVALVLLAVLALGWPGLALFDRADSVAGIPLLFLY
                     VYAVWALLIGLLRAWMRGAGED"
     gene            complement(3848..>4362)
                     /locus_tag="PLQ18_04450"
     CDS             complement(3848..>4362)
                     /locus_tag="PLQ18_04450"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004525489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNE00936.1"
                     /translation="YIVATGPLGADVATALLPADAALCDANFVLDYYRRSADHRLLFG
                     GRVSYSGLESPGLAAGMRRRLLAVFPQLPRTLAITHAWGGHVDITVNRAPHFGRIGSR
                     VWFAQGFSGHGMALSNLAGRVLAEAVSGQLERFDVFAGIPHRDFPGGGRLRVPLLMLA
                     MLAFRLRDRL"
BASE COUNT          651 a         1566 c         1539 g          606 t
ORIGIN      
        1 acgccgacct gcgcctgacc gagaccggcc gccggctcgg actggtcggc gatgtgcgct
       61 gggcggcgtt cgaggcacgg cgcgaggcga tcgagcgcga gacgcagcgc ctgcgcgagt
      121 gctgggtgcg cccggccgcg ctcgacccgg cgctggccga agccgtgctc ggcgggccgc
      181 tcgcgcgcga gacgcgcgcg ctcgacctgc tgcgacgccc ggaactcgat cacgccgcgg
      241 tgctgcgcct cgccggcctg ccggaaagcg acctcgatgc cgacgtgatc gaacaggtcg
      301 aaatccaggc gggctacgcc ggctacatcg aacgtgcgca ggccgacatc gcccgcgccc
      361 gccgccagga ggacgccgcg atcccggccg gcttcgacta cgccggcgtg cgcgggctgt
      421 cgagcgaggt gcgcgagaag ctcgcgcgcg tgcgccccga caccgtcggt caggccgggc
      481 gcattcccgg cgtcacgccg gcagcggtgt cgctgctgct cgtgcacctg aagcgcctgc
      541 gcgccgccgg ctgaggacgg cggcaagccg cctccgcgcg gggcacttgc cgctgcctct
      601 cccggtcaaa ttgccgcccg ccgcggtttt cggctagtgt cgccggctgc cggtgcaagt
      661 ttgcaccctg cggcagcccg ccggacgacg agagcaggcg ccgggcaccg gtgaatacca
      721 ccgacgaccg aggaatcacg atgaagctga acgtcaacgt cgaggccacg ccacaggagc
      781 tgcgcgagtt cctgggcctg cccaacgtgc agcccctgca gcaggaaatg atggagatca
      841 ttcgcgagaa catgctgaaa ggcgtacagg gtttcgatcc ggtatcgctg atgaagccgt
      901 tcatggccgg cgcgatgccg gtgcaatcca tcgaaaacct gcagaaggtg ttctgggaca
      961 ccttcaacaa ggccagcacc ggccagccgt cgcgtaccgg taccaccggc ggcagctgag
     1021 cggggcggga gcgagccggc atcagaccgg cgcgcggacg cgggtgcgcc ggggcgggcg
     1081 acccgcagag gggggagaca tgccgaagat actcgtggtc gatgacgagc ccaacatcct
     1141 gttgtcgctc gaattcctga tgaagcacgc cggtttcgag gtgcgcaccg ccaccgacgg
     1201 cgacgccgcg ctggccgcgg tcgccaccga ggtgcccgat ctcatcctgc tcgacgtgat
     1261 gatgccgcgg cgcgacggct acgaggtctg tcaggccgtg cgtgaaaacc cggcctgcgc
     1321 gcgcgtgaag atcatcatgc tcaccgccaa gggccgcgag atcgagcgcg agaagggcat
     1381 ggcgctcggg gccgacgact acatcgtcaa gcccttcgcg acgcaggaac tgctcgggcg
     1441 cgtgcgcgag ctgctcgccg gggcgacctg aggcgatccc gtcactggcc ggctcagggg
     1501 gccgccgtca tgaccgtccg aatcaagttc tgggcgctgc tgctcgcgcc gctcgcgttc
     1561 accggcctgt tcgcggccgt gtcgctgtgg aagctgcccg agatcgtcat cgatccgtac
     1621 atgcaggaga acgtccgcgc gttctacttc gccggcggca tccttgcgtg tctcgcctac
     1681 gccgtggtct gggccttcat cgatgccgcc ttcgtgcggc cgctgaaggc gctgctgcac
     1741 ggggcgcgca tcatcagccg cagcaacccg gcgcacgaac tgatgatgcc gcgcaaccac
     1801 ctgctcggcg aggtgccggt gatcgtgcag gagctcggcg ccagcctgca tcaggcgcgc
     1861 aaggagatcg ccgccgccac cgccaccggc acgcgcgaga tcgaggcgca gaagtcgcgg
     1921 ctcgaagtcg tgctgcgcga gctgagcgaa ggcgtgctgg tctgcgacgc cgatgcgcgc
     1981 gtgctgctct gcaacccggc cgcgcgccgg ctgctgccga cgcccgaggc gctcgggctc
     2041 ggccgttcga tctacggcct gctgacccgc cagccgatcg agcacacgct cgactggctg
     2101 ggcttccgcc agcagcaggc cggcgacggt gcgccgccgg cgaccgatgc cgagttcctg
     2161 tgctcggcgg ccgacgaatc gcggctgttc cagtgccgca tgagcctgct gccgagcagc
     2221 ggcgcggcgc ggcgcagctt cgtgctgacg ctgcgcgaca tcaccagcca ggtcgcaccg
     2281 ggcacgctcg gcccgagcgg cttcaagggt ctggtcgagg acctgcgccg ccagctcgcc
     2341 agcctgcgct tcgcggccga gtcgctgtcg ctgtatgcgg acatggagcc gggccagcgc
     2401 cgcgccttcg agcgcgtgat cttcgacgag agcgcggcgc tgaccgagcg cgtcgaattc
     2461 ctcgcccggc acgtgcgcct gaccttcgcc gcgcagtggc cgagcgccga cgtgctcagc
     2521 accgacgtga tcggcgcggc gctggcccgc ctcgaacgca gccgcgggcg catgccggtc
     2581 atcgtccagt ccggcgaccc gctgtggctg cactgcgacg gccagagcgt catgcagctc
     2641 cttgaattcg tgctgaccga gcttgccaac caggagccgg cggcgagctt cgagatcgaa
     2701 tgcctgctcg gcaaccggca ggtctacgtc gacatcgtct gggccggccg gccgctgccg
     2761 gcgcggctga tccaggactg gctggagcgg ccgctgcagc acgtcgccgg cgccgcgacc
     2821 gtcggcgaga tcctgcgccg ccaccattcc gacctctgga gccagccgca ccgcaagccg
     2881 ggcctcgcgg tgctgcgcat cccgctgccg tcctcgccgc ggcagtggag cgacgaggac
     2941 ggcgcgctgc ccgagcgccc ggagttctac gacttcgacc tcgaactgcg cccggcgcag
     3001 ttcggcgcgc tcgccgaccg gccgctgaag tcgctcgact acgtggtctt cgataccgag
     3061 accacggggc tgtcgccgtc gcgcggcgac gagatgatcc agatcgccgg cctgcgcgtc
     3121 gtgcagggcc gcatccgcga gggcgagacc ttcgaccggc tgatcaaccc cggcaagccg
     3181 atcccgaagg cctcgatcaa gttccacggc atcaccgatg ccatggtccg cgacaagccc
     3241 ggcgccgccg tgatcgtgcc gcagttccgc gccttcgtcg gcgagccgga cacggtactg
     3301 gtcgcacaca acgccgcctt cgacatgaag ttcctcgaac tgaaggaagc cgtgaccggc
     3361 gtgagcttcc gccagcagcc ggtgctcgac acgctgctgc tctccggcgc gatccacgac
     3421 tacaccgacg accacacgct cgacgccatc gccgagcgcc tcggcgtcga cgtgcacaac
     3481 cgccacaccg cgctcggcga cacgctggtc accgcgcagg tgttcgtgaa gctgctcgac
     3541 ctgctcgaag cccagggcat cgtcacgctc gggcaggcgc tggcgctgtc cgaacagatg
     3601 acggccgtgc gccgccagca ggcgaagttc tgacatggac gccggcgagc gcacggcccg
     3661 catcgtcgcg ctggtgctgc tggcggtgct cgcactcggc tggccggggc tcgcgctgtt
     3721 cgaccgcgcc gacagcgtcg ccggcatccc gctgctgttt ttgtacgtct acgcggtctg
     3781 ggcgctgctg atcgggctgc tgcgggcgtg gatgcgcggg gcgggcgagg actgatccgc
     3841 ccgcgcccta cagccggtcc cgcagccgga acgccagcat cgccagcatc agcagcggca
     3901 cgcgcagccg gccgccgccc ggaaagtccc ggtgcgggat gccggcaaac acatcgaagc
     3961 gttcgagctg gccgctgacc gcctcggcga gcacgcggcc ggcgaggttc gagagcgcca
     4021 tgccgtggcc ggaaaagccc tgcgcgaacc agacccggct gccgatgcgc ccgaagtgcg
     4081 gggcgcggtt gacggtgatg tcgacgtggc cgccccatgc gtgcgtgatc gcgagcgtgc
     4141 gcggcagttg cgggaacacc gcgagcaggc gccggcgcat gccggccgcg agtcccggcg
     4201 attccaggcc gctgtagctg acgcgcccgc cgaacagcag ccggtgatcg gccgagcgcc
     4261 ggtagtagtc gagcacgaag ttggcatcgc agagcgcggc gtccgccggc agcagcgccg
     4321 tcgccacgtc ggcaccgagc ggcccggtcg cgacgatgta gg
//
LOCUS       DAOQJH010000140         4357 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_12122_length_4357_cov_3.612273, whole genome shotgun sequence.
ACCESSION   DAOQJH010000140 DAOQJH010000000
VERSION     DAOQJH010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 4357)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4357)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4357
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12122_length_4357_cov_3.612273"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1495)
                     /locus_tag="PLQ18_04455"
     CDS             complement(<1..1495)
                     /locus_tag="PLQ18_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018387099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HNE00937.1"
                     /translation="MRCPCLILAAALWLGGPVAVADAPAPAPEAASGWTPKTAVQARE
                     FMVAAAHPMAVETGRDVIRRGGSAADAAIAMQLVLGLVEPQSSGLGGGAFVLYWDAAA
                     GQVKSLDGRETAPMEADGRLFLDADGQPLAFLAAVIGGRSVGVPGTPKLLAELHRRWG
                     RLPWAELFAPAIRLAREGFPVSPRLAGAIAALGDGGSHRDGFGNDAARELFLHADGTP
                     LRAGERLVNPAYADTLATLAAEGVGPFYQGPIAEDIVRTVGSHAANPGRMSTGDLAAY
                     TVRERVPVCAPYRQWRVCGMGPPSSGGVTLLQMLGILERLPPPPAGQPPALAALHRFA
                     EAGRLAYADRERYLADPDYALVPVEGLLDPDYLEVRAGLFDAQRSLGRAAAGSPPGAP
                     SRGEARAAELPSTSHLVIVDRDGNAVSMTTTIENGFGSRLLVRGFLLNNELTDFAFAP
                     TGADGQPVANRVEPGKRPRSSMAPTLVFGPDGRLLLALGSPGGSSIIN"
     gene            complement(1556..1927)
                     /locus_tag="PLQ18_04460"
     CDS             complement(1556..1927)
                     /locus_tag="PLQ18_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HNE00938.1"
                     /translation="MSDRDPSVPELERFEPGPRYSEAVAAGGLVFLAGQVPEDPEAGI
                     TQQAAEVLAAVERALALAGSTRARLVSATIYLREMADYAAFNAVWDAWLLPHPAPARA
                     CVQALLADPRWRVEVQVVAAR"
     gene            complement(2002..2238)
                     /locus_tag="PLQ18_04465"
     CDS             complement(2002..2238)
                     /locus_tag="PLQ18_04465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE00939.1"
                     /translation="MEDLKTLKHTGRTPSLDPPVLQRLRELHAALARLPGGRDVHDAL
                     DPLLRGLSPDQRERMRGRITGRIFSRRSSSSRGL"
     gene            complement(2256..3020)
                     /locus_tag="PLQ18_04470"
     CDS             complement(2256..3020)
                     /locus_tag="PLQ18_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HNE00940.1"
                     /translation="MRRVVFNQKGGVGKSTITCNLAAIAAARGRRTLVVDLDPQANSS
                     HYLLGGAADGEGPTVADFFDDMLSFKFNPPGPEHFARPTPFVNLFVMPSQGRLEELGP
                     KLESRYKMFKLREALESLRGFDEIFIDTPPAVNFFTRSALIAAERCLIPFDCDSFSRR
                     ALYLLLDNVREMQADHNRGLQVEGIVVNQFQSQARLPRQLVEELRAEGLPVLDAFLST
                     SVKIRESHERALPMLHLDPRHKLTQEFEALYGLLAA"
     gene            complement(3134..4009)
                     /locus_tag="PLQ18_04475"
     CDS             complement(3134..4009)
                     /locus_tag="PLQ18_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004345841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HNE00941.1"
                     /translation="MPLPLLLALLLSFPIPARADLTVTDDTGRAVQLPAPARRIVAVA
                     PHAAELLHAAGAGAALVGVVQYADFPPAVRTLPSVGSYTGFDLEALLALKPDLVIGWD
                     SGNPPAQLARIEALGIPVFRTEARVADDIATDIEKLGELAGTAAVATQAAAAFRQRLA
                     QLRREHAGRRPVRVFYQVWARPLVTLNGEHPVSHVLADCGGVNVFAALPALAPQIDEE
                     AVLAADPEVIIGGGMDGERPEWLEDWRRRWPQLAAVRGGHLYAVPPDWLHRSGPRLLD
                     GMERVCAQLDAARGG"
     gene            complement(4011..>4357)
                     /locus_tag="PLQ18_04480"
     CDS             complement(4011..>4357)
                     /locus_tag="PLQ18_04480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE00942.1"
                     /translation="VSGWDEYAGLRARLLMDDRFAGTGQPQRLLFQQHLPVDLVPWGH
                     QDANGSIAWPPDFAVRMSVIGYDEAYAANPLMPAAAGPDERLDCARRLLQAYADGITD
                     ALPHIDPAHGRT"
BASE COUNT          665 a         1574 c         1569 g          549 t
ORIGIN      
        1 aattgatgat gctgctgccg cccggtgagc cgagcgcgag cagcagccgg ccgtccgggc
       61 cgaacaccag cgtcggcgcc atcgacgagc gcgggcgctt gccgggctca acgcggttgg
      121 cgaccggctg gccgtcggca ccggtcggcg cgaacgcgaa gtcggtcagc tcgttgttga
      181 gcaggaagcc gcgcaccagc agccggctgc cgaagccgtt ctcgatcgtc gtggtcatgc
      241 tgacggcgtt gccgtcgcga tcgacgatga ccagatgcga ggtcgacggc agttcggcgg
      301 cacgcgcctc gccgcgcgac ggcgcgccgg gcggactgcc ggccgcagcg cggccgagcg
      361 agcgctgcgc atcgaacagc ccggcgcgca cttcgaggta atccggatcg agcaggccct
      421 cgaccggcac cagcgcgtag tccggatcgg ccagatagcg ttcgcgatcg gcataggcga
      481 gccgcccggc ctcggcgaag cggtgcagcg ccgcgagcgc cggcggctgc ccggccgggg
      541 gcggcggcag gcgttcgagg atgccgagca tctgcagcag cgtcacgccg cccgaactcg
      601 gcggccccat gccgcacacc cgccactgcc ggtacggcgc acacaccggc acgcgctcgc
      661 gcaccgtgta tgcggccaga tcgcccgtgc tcatgcgccc gggattggcc gcgtgcgagc
      721 cgaccgtgcg cacgatgtcc tcggcgatcg gcccctgata gaacggcccg acgccttcgg
      781 cggccagcgt cgccagcgtg tcggcgtagg ccggattcac gagccgctcg ccggcgcgca
      841 gcggcgtgcc gtcggcgtgc aggaacagct cgcgcgcggc atcgttgccg aagccgtcgc
      901 ggtgactgcc gccatcgccg agcgcggcga tcgcgccggc gagccgcggc gacaccggga
      961 agccctcgcg cgccagccgg atcgccggcg cgaacagctc ggcccacggc agccggcccc
     1021 agcgccggtg cagctcggcg agcagcttcg gcgtgccggg cacgccgacc gaacggccgc
     1081 cgatcacggc agcgaggaag gcgagcggct ggccgtcggc atcgaggaac agccggccgt
     1141 cggcctccat cggcgcggtc tcgcggccgt cgagcgactt cacctgcccg gccgcggcat
     1201 cccagtacag cacgaaggcg ccgccgccga gccccgagga ctgcggctcg acgaggccga
     1261 gcaccagttg catcgcgatc gcggcatcgg cggccgaacc gccgcggcgg atcacgtcgc
     1321 gaccggtctc gaccgccatc ggatgcgcgg cggcgaccat gaactcgcgc gcctgcacgg
     1381 ccgtcttcgg cgtccagccg ctcgcggcct cgggcgccgg cgccggtgca tcggcaacgg
     1441 cgaccgggcc gccgagccag agcgcggcgg cgaggatcag gcacggacag cgcatcgaca
     1501 atctccggaa catgccagcg aaaggcagga aatgaacggg gaaccacccg cggcttcagc
     1561 gcgccgcaac cacctgcacc tcgacgcgcc agcgcggatc ggcgagcagc gcctgcacgc
     1621 aggcgcgcgc cggtgccgga tgcggcagga gccacgcatc ccagacagcg ttgaacgccg
     1681 cgtagtcggc catctcgcgc aggtagatgg tcgccgacac cagccgcgcg cgcgtgctgc
     1741 cggccagcgc cagcgcccgc tcgacggcgg cgagcacctc ggcggcctgc tgcgtgatgc
     1801 cggcctccgg gtcctcgggc acctgaccgg cgaggaagac cagcccaccg gccgccacgg
     1861 cctcggaata gcgcgggccg ggctcaaaac gctcgagctc cggcacggac ggatcgcgat
     1921 cggacacggt gttcccctcg ttcgcagccc cggcggaacg cggcgcaccg acccggggct
     1981 gcgggcggcg cgcctcgggc ctcacagccc gcgtgatgat gacgagcgcc ggctgaagat
     2041 gcgcccggtg atccgcccgc gcatgcgctc gcgctggtcc ggcgacaggc cgcgcagcag
     2101 cggatcgagc gcgtcgtgaa catcacggcc gccgggcagc cgcgcgagtg ccgcgtgcag
     2161 ctcgcgcagg cgctgcagta ccggcggatc gaggctgggc gtccgcccgg tgtgcttcag
     2221 ggtcttgagg tcttccatgg ccgccgtgtg caagctcagg ccgccagcag gccgtacaag
     2281 gcctcgaatt cctgcgtgag cttgtgacgc gggtcgaggt gcagcatcgg cagtgcgcgt
     2341 tcgtgcgatt cgcggatctt gaccgaggtc gacaggaagg catcgagcac cggcagaccc
     2401 tcggcgcgca gttcctcgac cagctggcgc ggcagccgcg cctgcgactg gaactggttg
     2461 acgacgatgc cctcgacctg caggccgcgg ttgtggtcgg cctgcatctc gcggacgttg
     2521 tcgagcagca gatacagcgc gcggcgcgag aagctgtcgc agtcgaacgg gatcaggcag
     2581 cgctcggcgg cgatcagcgc cgaacgcgtg aagaagttca cggccggcgg cgtgtcgatg
     2641 aagatctcgt cgaagccgcg caggctttcg agcgcctcgc gcagcttgaa catcttgtag
     2701 cgcgattcga gcttgggccc gagttcctcc agccggccct gcgagggcat cacgaacagg
     2761 ttcacgaacg gcgtcggccg cgcgaagtgt tcgggtccgg gcgggttgaa tttgaacgac
     2821 agcatgtcat cgaagaaatc cgcgaccgtc ggcccctcgc cgtcggcggc cccgccgagc
     2881 aggtagtgcg aggaattggc ctgcggatcg agatcgacga ccagcgtgcg ccggccgcgg
     2941 gccgcggcga tcgccgcgag attgcaggtg atcgtcgact tgccgacccc gcctttctgg
     3001 ttgaacacga cgcgacgcat gatctttgtg gtccttcccc cgggtggtgc gccagtttaa
     3061 cgaaccgtga cagggaaatt ccgcattcgt tgcgctgcgt caaggccgga ccggccggcc
     3121 ggcagaccgc cgttcagccg ccgcgcgccg catcgagctg cgcgcagacc cgctccatgc
     3181 catcgagcag gcgcggcccg gagcgatgca gccagtccgg aggcaccgcg tacagatgcc
     3241 cgccccgcac ggccgccagt tgcggccagc gccggcgcca gtcctccagc cactccgggc
     3301 gctcgccgtc catgccgccg ccgatgatga cctcgggatc ggcggcgagc acggcttcct
     3361 cgtcgatctg cggcgccagt gccggcagcg ccgcgaacac gttgacgccg ccgcagtcgg
     3421 cgagcacgtg gctgaccgga tgctcgccgt tcagcgtcac cagcggcctg gcccagacct
     3481 gatagaacac gcgcaccggc cgccgcccgg catgctcgcg acgcagctgc gcgagccgct
     3541 gccggaacgc cgccgccgcc tgcgtcgcca ccgccgcggt gccggccagc tcgccgagct
     3601 tctcgatgtc ggtggcgatg tcatcggcga cgcgcgcctc ggtgcggaac accggaatgc
     3661 cgagcgcctc gatgcgcgcg agctgcgccg gcggattgcc gctgtcccag ccgatcacca
     3721 gatcgggctt cagcgccagc agcgcttcga ggtcgaagcc ggtatacgaa ccgacgctcg
     3781 gcagcgtgcg caccgccggc ggaaaatccg cgtactgcac gaccccgacc agcgccgccc
     3841 cggcaccggc ggcatggagc agctcggcgg catgcggcgc caccgcaacg atccgccgcg
     3901 ccggcgccgg cagttgcacg gcacggccgg tgtcgtcggt gacggtcaga tcggcacggg
     3961 cgggaatggg gaagctcagc aggagcgcga gcagcagcgg cagaggcatg tcaggtgcgt
     4021 ccgtgtgcag ggtcgatgtg cgggagtgca tccgtgatgc cgtcggcata ggcctgcagc
     4081 aggcgccgtg cgcaatccag ccgctcatcc ggccccgcgg cggccggcat cagcggattt
     4141 gccgcatagg cctcgtcgta gccgatgacg ctcatgcgca cggcgaagtc cggcggccag
     4201 gcgatgctgc cgttcgcatc ctgatggccc cagggcacga gatcgaccgg cagatgctgc
     4261 tggaacagca gccgctgcgg ctgtccggtg ccggcgaagc ggtcgtccat caacagccgg
     4321 gcgcgtaatc cggcgtattc gtcccagccc gacacgg
//
LOCUS       DAOQJH010000141         4354 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_12138_length_4354_cov_3.808560, whole genome shotgun sequence.
ACCESSION   DAOQJH010000141 DAOQJH010000000
VERSION     DAOQJH010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 4354)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4354)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4354
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12138_length_4354_cov_3.808560"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..501)
                     /locus_tag="PLQ18_04485"
     CDS             complement(<1..501)
                     /locus_tag="PLQ18_04485"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015280264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HNE00943.1"
                     /translation="MARCFAIVPAAGVGRRMGAAIPKQYLPLAGRCVLEHTLERLLAE
                     PRIERIVVALGPEDGWWPALKLSDPARIRRVDGGTERADSVLNGLRALEGLAGTDDWA
                     LVHDAARPCLAAEDLARLFTALADDPLGGLLAVPVRDTMKRAAADGCVEHTVERERLW
                     HALTPQM"
     gene            complement(501..962)
                     /gene="ftsB"
                     /locus_tag="PLQ18_04490"
     CDS             complement(501..962)
                     /gene="ftsB"
                     /locus_tag="PLQ18_04490"
                     /inference="COORDINATES: protein motif:HMM:NF002058.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HNE00944.1"
                     /translation="MRLFLAALIALLLGLNYLLWMTDDRGLRQMRTLERAVADQKSEN
                     TELADRNKSLEAEVRDLKQGNAAIEERARTELGMVRGDETFYRILDEAPSSPPSALTK
                     PGTAIHARPQAPSAPAAVAPATPKPDAKPAAKPAVPRPPASKPAPDSGRPD"
     gene            complement(1045..2337)
                     /gene="eno"
                     /locus_tag="PLQ18_04495"
     CDS             complement(1045..2337)
                     /gene="eno"
                     /locus_tag="PLQ18_04495"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011629577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HNE00945.1"
                     /translation="MSAIQSLIGREILDSRGNPTVQVEVELASGARGTAAVPSGASTG
                     TREAIELRDGDAGRYLGKGVLKAVGHVNGEILKAVRGMEAADQAALDARLIELDGTAN
                     KGRLGANALLGVSLAVAHAAASEKRVPLYRHINPDGPYVLPTPMMNILNGGAHADNSV
                     DIQEFMVMPVGAPSFSEALRCGAEIFHALKKVLNGRGLATGVGDEGGFAPNLPSNEAA
                     LEVVMEAIGKAGYRAGDDVMLALDCASSEFYRNGRYTLEADGKAYSSLEWAGVLADLA
                     GRYPIISIEDGMDESDWAGWAELTRLLGERIQLVGDDLFVTNTEILQRGIREGIANSI
                     LIKVNQIGTLTETLNAIRMADEAGYSSISSHRSGETEDTTIADLAVATTARQIKTGSL
                     SRSDRVAKYNRLLVIESQLGADAVYAGRKAFPRKLKLG"
     gene            complement(2444..3280)
                     /gene="kdsA"
                     /locus_tag="PLQ18_04500"
     CDS             complement(2444..3280)
                     /gene="kdsA"
                     /locus_tag="PLQ18_04500"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HNE00946.1"
                     /translation="MKLCGFEVGLDRPLFLIAGPCVIESRELAFDTAGRLKEMCAALG
                     MPFIYKSSFDKANRSSHASYRGPGLEAGLAILEAVRRELDVPVLTDVHEDTPLAEVAA
                     VVDVLQTPAFLCRQTNFIQAVARAGRPVNIKKGQFLAPWDMKNVADKALATGNTQIMV
                     CERGVSFGYNNLVSDPRALAVMRDTGCPVVFDATHSVQLPGGQGTSSGGQRQHVPVLA
                     RAAVAVGVAGLFMETHPQPEQALSDGPNAWPLGRMQGLLETLVELDALVKRRGFDEND
                     PG"
     gene            complement(3277..>4354)
                     /locus_tag="PLQ18_04505"
     CDS             complement(3277..>4354)
                     /locus_tag="PLQ18_04505"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005370900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HNE00947.1"
                     /translation="KPTQHSVKELRSIGIQPDILLCRCEHEIPAEERRKIALFTNVEA
                     KAVFSAVDADTIYRIPLLLHQQGLDDVVCQKLGLRADPANLDEWTRVVGQIEAARGDT
                     IEIAMVGKYVDLADAYKSLNEALFHAGFHTGHKVAIRYIDSEQLEREGTGCLAGMHAI
                     LVPGGFGERGVEGKIAAVRHAREQRIPYLGICLGMQVAVIEYARNVAGLEGAHSTEFR
                     KNATHPVIALITEWTNREGAVERRSEESDLGGTMRLGAQPARLVEGTLAHRVYGKDVI
                     VERHRHRYEFNDGYVPVLEKSGLRFSGFSQDGNLVEMIELPDHPWFLACQFHPEFTST
                     PRYGHPLFRAFVEAAVAHREGKLA"
BASE COUNT          627 a         1455 c         1561 g          711 t
ORIGIN      
        1 catctgcggc gtcagcgcat gccagagccg ctcgcgctcg acggtgtgct cgacgcagcc
       61 atcggccgcg gcgcgcttca tcgtgtcgcg caccggcacc gccagcaggc cgccgagcgg
      121 atcgtcggcg agggcggtga acagccgtgc gaggtcttcg gccgccagac acgggcgtgc
      181 cgcgtcgtgg accagcgccc agtcatcggt cccagccagc ccttcgagcg cacgcaaacc
      241 gttcagtacc gaatcggcgc gctcggtgcc gccgtcgacg cgccggatgc gtgccggatc
      301 ggagagcttc agcgccggcc accagccgtc ctcggggccg agcgcgacga cgatgcgctc
      361 gatgcgcggc tcggcgagca ggcgttcgag cgtgtgttcg agcacgcagc ggccggcgag
      421 cggcagatac tgtttcggga tcgccgcgcc catgcgcctg ccgacgccgg cagccggaac
      481 gatggcgaag cagcgcgcca tcagtcgggc cggccgctgt cgggcgccgg tttcgaagcc
      541 ggcgggcgcg ggacggccgg tttcgcggcg ggtttcgcat cgggcttcgg agtggccggc
      601 gccacggcgg cgggcgcgga cggggcctgc ggccgcgcgt ggatcgcggt gccgggtttg
      661 gtcagcgccg agggcggcga actcggggct tcgtcgagga tgcgatagaa ggtctcgtcg
      721 ccgcgcacca tgccgagttc ggtgcgggcc cgctcctcga tcgcggcatt gccctgcttg
      781 agatcgcgga cttcggcttc cagcgacttg ttgcgatcgg cgagttcggt gttttcggat
      841 ttctggtcgg cgaccgcacg ttcaagcgtg cgcatctggc gcagcccgcg gtcgtcggtc
      901 atccacagca ggtaattcag cccgagcagc agcgcgatca gggcagcgag gaagaggcgc
      961 attcggggcc gatcgaacct gcaaaacggg gggaaatcag gcggtgcgca cgcgcggcgg
     1021 cgtgcgcacc ggagagccgc gggctcagcc cagcttcagc ttgcgcggga aggccttgcg
     1081 gccggcgtag acggcatcgg caccgagctg tgactcgatg accagcagcc ggttgtactt
     1141 ggcgacgcgg tcggagcggc tcagcgagcc ggtcttgatc tggcgcgccg tggtcgcgac
     1201 cgcgaggtcg gcaatggtcg tgtcctcggt ctcgccggag cggtgcgagc tgatcgagct
     1261 gtagccggcc tcatcggcca tgcggatcgc gttcagcgtc tcggtcagcg tgccgatctg
     1321 gttgaccttg atcaggatcg agttggcgat gccttcgcgg atgccgcgct gcaggatctc
     1381 ggtgttggtc acgaacaggt cgtcaccgac gagctggatg cgctcgccga gcagccgggt
     1441 cagttcggcc cagccggccc agtcggattc atccatgccg tcctcgatcg aaatgatcgg
     1501 atagcggccg gcgagatcgg cgagcacgcc ggcccattcg agcgacgaat atgccttgcc
     1561 gtcggcttcg agcgtgtagc ggccgttcct gtagaactcg gagctggcgc agtcgagtgc
     1621 gagcatcacg tcgtcgccgg cgcggtagcc ggccttgccg atcgcttcca tgaccacttc
     1681 gagcgcggcc tcgttcgacg gcaggttcgg cgcgaagcct ccctcgtcgc cgacgccggt
     1741 cgccaggccg cggccgttca ggaccttctt cagcgcgtgg aagatctcgg cgccgcaacg
     1801 cagcgcttcc gagaagctcg gcgcaccgac cggcatgacc atgaattcct ggatgtcgac
     1861 cgagttgtcg gcatgcgcgc cgccgttgag gatgttcatc atcggcgtcg gcagcacgta
     1921 ggggccatcc gggttgatgt gccggtacag cggcacgcgc ttctcgctgg ccgcggcatg
     1981 cgcgaccgcg agcgacacgc cgagcagcgc gttggcgccc aggcgcccct tgttggcggt
     2041 gccgtcgagc tcgatcagcc gcgcatcgag cgccgcctga tcggccgcct ccatgccccg
     2101 caccgccttc aggatctcgc cgttgacgtg gccgacggcc ttcagcacgc ccttgccgag
     2161 gtagcgcccg gcatcgccgt cgcgcagctc gatcgcctcg cgcgtgccgg tcgaggcccc
     2221 cgagggcacg gcggccgtgc cgcgcgcgcc gctcgcgagc tcgacctcga cctgcacggt
     2281 cgggttgccg cgcgagtcga ggatttcgcg gccgatcagg gattgaatcg cggacatcgt
     2341 cacttctcct gctgtcttgt cggaaacacc gggttcgcgg cggttgcgcg tcgcccggcc
     2401 ggggatggtg atatcggggg aaatcctgaa acctgccggg aaatcagccc ggatcgtttt
     2461 cgtcgaagcc gcggcgcttc accagcgcat cgagttcgac cagggtttcg agcaggccct
     2521 gcatgcgtcc gagcggccag gcgttcgggc cgtcggacag cgcctgttcg ggctgcggat
     2581 gagtttccat gaacagcccg gccacgccga cggcgaccgc ggcacgggca agcaccggca
     2641 cgtgctggcg ctggccgccg gagctcgtgc cctgcccgcc cggcagctgc accgaatgcg
     2701 tggcgtcgaa caccaccgga cagccggtgt cgcgcatcac ggccagcgcg cgcgggtcgg
     2761 aaaccaggtt gttgtagccg aaggagacgc cgcgctcgca gaccatgatc tgcgtgttgc
     2821 cggtcgcgag cgccttgtcg gcgacgttct tcatgtccca gggcgcgagg aactggccct
     2881 tcttgatgtt gaccggccgc ccggcgcgcg cgaccgcctg gatgaagttg gtctggcggc
     2941 acaggaaggc cggcgtctgc agcacgtcga cgacggcggc gacctcggcg agcggcgtgt
     3001 cctcgtgcac gtcggtcagc accggcacgt cgagctcgcg ccggacggct tcgaggatcg
     3061 cgagccccgc ttccagcccc ggcccgcggt agctcgcgtg cgagctgcgg ttggccttgt
     3121 cgaagctcga cttgtagatg aagggcatgc cgagcgcggc gcacatctcc ttgaggcggc
     3181 cggcggtgtc gaaggccagc tcgcgcgatt cgatcacgca ggggccggcg atcaggaaca
     3241 gcggccggtc gaggccgacc tcgaatccgc agagcttcat gcgagcttgc cctcgcggtg
     3301 cgcgaccgcc gcctcgacga aggcgcggaa cagcgggtgg ccgtagcgcg gcgtcgatgt
     3361 gaactcggga tggaactggc aggccaggaa ccacgggtga tcgggcagct cgatcatctc
     3421 gaccaggttg ccgtcctgcg agaagcccga gaagcgcagg ccggatttct ccagcaccgg
     3481 cacgtagccg tcgttgaact cgtagcggtg gcgatggcgc tcgacgatca cgtccttgcc
     3541 gtagacgcgg tgcgccagcg tgccctcgac cagccgtgcc ggctgggcgc cgaggcgcat
     3601 cgtgccgccg aggtcggatt cctcggagcg acgctcgacc gcaccttccc ggttcgtcca
     3661 ttcggtgatc agcgcgatga ccggatgcgt cgcgttcttg cgaaactcgg tcgaatgcgc
     3721 gccttcgagg ccggcgacgt tgcgcgcgta ctcgatgacg gcgacctgca tgccgaggca
     3781 gatgccgaga tacggaatgc gctgctcgcg cgcatggcgc accgcggcga tcttgccttc
     3841 gacgccgcgc tcgccgaagc cgccgggcac caggatcgcg tgcatgccgg ccaggcagcc
     3901 ggtgccctcg cgttcaagct gctcggagtc gatgtagcgg atcgcgacct tgtggccggt
     3961 gtggaagccg gcgtggaaca gcgcctcgtt cagcgacttg taggcatcgg cgagatcgac
     4021 gtacttgccg accatcgcaa tctcgatcgt gtcgccgcgc gccgcctcga tctgcccgac
     4081 cacgcgcgtc cattcgtcga gattcgccgg gtcggcgcgc aggccgagct tctggcagac
     4141 cacatcgtcg agcccctgct ggtgcagcag cagcgggatg cggtagatcg tgtcggcgtc
     4201 gacggcactg aacaccgcct tggcctcgac gttcgtgaac agcgcgatct tgcgccgctc
     4261 ctcggccggg atttcgtgct cgcagcggca gagcaggatg tcgggctgga tgccgatcga
     4321 gcgcagttcc ttgaccgagt gctgcgtcgg cttg
//
LOCUS       DAOQJH010000142         4330 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_12233_length_4330_cov_3.711111, whole genome shotgun sequence.
ACCESSION   DAOQJH010000142 DAOQJH010000000
VERSION     DAOQJH010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 4330)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4330)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4330
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12233_length_4330_cov_3.711111"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..919)
                     /gene="gcvT"
                     /locus_tag="PLQ18_04510"
     CDS             complement(<1..919)
                     /gene="gcvT"
                     /locus_tag="PLQ18_04510"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HNE00948.1"
                     /translation="MSAVETGLARTPLHDLHLELGARMVPFAGYSMPVQYPAGVLKEH
                     QHTRMAAGLFDVSHMGQVRLDGAQAAAALETLVPVDVLGLPAGRQRYAVFTNDAGGIL
                     DDLMIVNAGDHLFLVVNAACKAQDLAHLRARLEPAVTVTELADRALLALQGPAAAEVF
                     LRLAPAAAGMKFMDFRTLEVAGIPCFASRSGYTGEDGFEISVPAARAVELARALLAEP
                     EVAPIGLGARDSLRLEAGLCLYGHELDPTTTPIEAAIGWAIQKVRRTGGARAGGFPGA
                     DTILAQFDAGTARLRVGLAGSTRAPVREGA"
     gene            complement(950..1471)
                     /locus_tag="PLQ18_04515"
     CDS             complement(950..1471)
                     /locus_tag="PLQ18_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014992864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HNE00949.1"
                     /translation="MTTNPPAAARRPVSPRLWNMLGFLACLAGMMYALYAQYFLHLEP
                     CPLCIFQRIALIALGAVFIIAALHDPQGWGSRIYALLILLVAAAGAAIAARHAWLQSL
                     PPEQVPACGPGLDYLWQNFPIADLLNTVLRGSGECAKIDWELFHLSMPWWTLFMFVGL
                     GLAGFVRNLLGTR"
     gene            complement(1578..1913)
                     /locus_tag="PLQ18_04520"
     CDS             complement(1578..1913)
                     /locus_tag="PLQ18_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017937774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SelT/SelW/SelH family protein"
                     /protein_id="HNE00950.1"
                     /translation="MTDTNTGTDAVKNPAPAARPRIEIVYCTQCRWLLRAAWLAQELL
                     STFDTDLGEVALIPGTGGVFEMRCDGVMIWSRKAEGRFPEAKEVKQRVRDCIDPGRDL
                     GHSDRPRTG"
     gene            complement(1919..2641)
                     /gene="mtgA"
                     /locus_tag="PLQ18_04525"
     CDS             complement(1919..2641)
                     /gene="mtgA"
                     /locus_tag="PLQ18_04525"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014504061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HNE00951.1"
                     /translation="MSDHAPESGPRRRLRLRTLLLALPLAGLLLSLLLVLPLRWLAPV
                     TSAFMIAVRLDDEAGRWPEYRWVPYEAISAQLALAVLAGEDQRFAEHHGFDTVEIERA
                     LSAADQGGKLRGASTISQQVAKNLFLWSGRSYVRKALEAWFTLLIEACWSKRRILEMH
                     LNLAQYGPRTFGAEAAAQRFFGKPAWDLDAGEAARLAAVLPNPRLYRVEAPNATVRTR
                     ERHIRTQMRNLGGTGFLDRLSE"
     gene            2717..>4330
                     /locus_tag="PLQ18_04530"
     CDS             2717..>4330
                     /locus_tag="PLQ18_04530"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNE00952.1"
                     /translation="MFDFKRLSIRNKLMAIMLLTTGSVLALATLVMVVNEALTLERST
                     RQQLAALADVIAASARGPMSLSDPVTAYDMLEALRSKGNIVYAQLRNPDGSVFAEFRR
                     EHDARIPDYPPPGDSETGVFQEAGQLIHYQRPVLLEWRRVGTLQLAADFGELYASLGR
                     YSLLLLMMMLTTFTFAVLIAAKLQRLISEPLLALHAATQRVREQRDYSVRVHHDADDE
                     TGALVRGFNDMLEQIRQRDLELARLAERLGAEVEHRTQELSGANRELQSMVGELRSEK
                     ERAEAASLAKTQFLASISHEIRTPMNGVLGMAELLLHTALDHRQRHLSTMIQRSGRTL
                     LGIINDILDQSKIEAGRLELDAEEFALREHLEQTIELYAEDARRHGLAFRADYAPHLP
                     RRVIGDPGRLAQVLGNLLANAIKFTPHGSITVHVDALPGAGPEVTIGCEVIDTGIGIA
                     PADQARIFEAFTQTDTSATRRYGGTGLGLTIARQIVQRMGGHIGLSSAPDAGSTFWFA
                     VQLRRVQTPAALEEHALRSARVLLVGLGAV"
BASE COUNT          671 a         1532 c         1540 g          587 t
ORIGIN      
        1 gtgccccctc gcgcaccggc gcgcgcgtgc tgccggcaag gccgacgcgc agccgcgccg
       61 tgccggcgtc gaactgcgcg aggatggtgt ccgcgcccgg aaacccgccg gcgcgcgcac
      121 cgcccgtgcg ccgcaccttc tggatcgccc agccgatcgc cgcctcgatg ggggtcgtcg
      181 tcgggtcgag ttcgtggccg tacaggcaga gcccggcttc gaggcgcagc gaatcgcgcg
      241 cgccgagacc gatcggcgcg acctcgggct cggcgagcag cgcacgcgcc agttcgacgg
      301 cgcgggcagc cggcaccgag atctcgaagc cgtcctcgcc ggtatagccg gagcggctcg
      361 cgaaacacgg gatgccggcg acctccagcg tgcggaagtc catgaacttc atgccggcgg
      421 cggccggggc cagacgcagg aacacttcgg cggcggccgg cccctgcagc gcgagcagtg
      481 cgcggtcggc gagttccgtg accgtgaccg ccggttcgag ccgcgcgcgc aggtgcgcga
      541 ggtcctgcgc cttgcacgcg gcgttgacga cgaggaacag gtgatcgccg gcgttgacga
      601 tcatcaggtc atcgaggatg ccgccggcgt cgttcgtgaa cacggcgtag cgctggcgcc
      661 cggccggcag gccgagcacg tcgaccggca ccagggtttc gagcgcggcg gcggcctgtg
      721 caccgtcgag gcgcacctgg cccatgtgcg agacatcgaa caggccggcg gccatgcgcg
      781 tgtgctggtg ctccttcagc acgccggcgg ggtattgcac cggcatcgag tagccggcga
      841 acggcaccat tctcgcgccg agttcgaggt gcaggtcgtg gagcggggtg cgggcgagac
      901 cggtttcgac agcgctcatg caggcatctc cggatcaggg gcggggcgct cagcgcgtgc
      961 cgagcaggtt gcgcacgaaa ccggccagac cgaggccgac gaacatgaac agcgtccacc
     1021 acggcatcga caggtggaac agctcccagt cgatcttcgc gcactcgccg ctgccccgga
     1081 gcacggtgtt cagcaggtcg gcgatcggga agttctgcca caggtaatcg agtcccggcc
     1141 cgcaggccgg cacctgctcg ggcggcaggc tctgcagcca ggcgtggcgc gcggcgatcg
     1201 ccgcgccggc agcggcgacg agcaggatca gcagcgcata gatgcgcgag ccccagccct
     1261 gcggatcgtg cagcgcggcg atgatgaaca ccgcgccgag cgcgatcagc gcgatgcgct
     1321 ggaagatgca gagcggacag ggttcgaggt gcaggaagta ttgcgcgtac agcgcgtaca
     1381 tcatgccggc caggcaggcg aggaaaccca gcatgttcca gagccggggc gacaccgggc
     1441 gccgggcagc ggcgggcggg ttggtcgtca tcggaggcag acctcggaca ggagcgggaa
     1501 gcggaaaacg ggcatgcacg ccggtgcgga tgacggcgat tatcgcaccg gtgcaggaat
     1561 cacgggcgca tggctgctca gccggtgcgc ggccggtcgc tgtggccgag atcgcgcccg
     1621 gggtcgatgc aatcgcgcac gcgctgcttg acctccttgg cctccggaaa gcgcccctcg
     1681 gccttgcgcg accagatcat cacgccgtcg cagcgcatct cgaacacgcc gccggtgccg
     1741 ggaatcagcg ccacctcgcc gagatcggta tcgaaggtgc tgagcagctc ctgcgcgagc
     1801 cacgcggcgc gcagcagcca gcggcactgc gtgcagtaga cgatctcgat gcgcgggcgg
     1861 gcggcggggg cggggttctt tacggcgtcg gtgccggtgt tggtgtcggt catgcggttc
     1921 actcgctcag gcgatccaga aagcccgtgc cacccagatt gcgcatctgc gtgcggatgt
     1981 ggcgttcgcg ggtgcgcacc gtggcgttcg gtgcctcgac ccggtacagc cgcggattcg
     2041 gcagcaccgc cgccagccgt gcggcctcgc cggcgtcgag gtcccaggcc ggcttgccga
     2101 agaagcgctg cgcggcggcc tcggcgccga aggtgcgcgg accgtactgc gcgaggttca
     2161 ggtgcatctc caggatgcgc cgcttgctcc agcaggcctc gatcagcagc gtgaaccagg
     2221 cctccagggc cttgcgcacg tagctgcgcc cggaccacag gaacaggttc ttcgccacct
     2281 gctggctgat cgtgctcgcc ccgcgcagct tgccgccctg atcggccgcg ctcagtgcgc
     2341 gctcgatctc gacggtatcg aagccgtgat gttcggcgaa gcgctggtcc tcgccggcga
     2401 gcacggccag cgcgagctgc gccgaaatcg cctcgtacgg cacccagcgg tattccggcc
     2461 agcgtccggc ctcgtcgtcg aggcgcacgg cgatcatgaa cgcgctggtc acgggcgcga
     2521 gccagcgcag cggcagcacc agcagcagcg acagcagcag gccggcgagc ggcagggcca
     2581 gcagcagcgt gcgcaggcgc aggcggcggc gcggaccgga ttcgggggcg tggtcgctca
     2641 tgcgccgagt atgcccggcg cgcgcggcgg gcgggcatgc gtagaatcgg cgcacgcccg
     2701 tcgtccgcag gcagccatgt tcgatttcaa gcgtctgtcc atccgcaaca agctcatggc
     2761 gatcatgctg ctgaccaccg gcagcgtgct ggcgctggcg acgctggtga tggtggtcaa
     2821 cgaggcgctg acgctggagc gctcgacccg ccagcagctc gccgcgctcg ccgacgtgat
     2881 cgctgccagc gcccgcgggc cgatgtcgct cagcgatccc gtcaccgcct acgacatgct
     2941 cgaagccctg cgcagcaagg gcaacatcgt ctacgcgcag ctgcgcaacc ccgacggcag
     3001 cgtgttcgcc gagttccgcc gcgagcacga cgcgcgcatc ccggactacc cgccgcccgg
     3061 cgacagcgag accggcgtgt tccaggaggc cggccagctc atccactacc agcgcccggt
     3121 gctgctcgaa tggcggcgcg tcggcacgct gcagctcgcc gccgacttcg gcgagctgta
     3181 cgcgagcctc gggcgctact cgctgctgct gctgatgatg atgctgacca ccttcacgtt
     3241 cgcggtgctg atcgccgcca agctgcagcg cctgatctcc gaaccgctgc tcgcgctgca
     3301 cgccgcgacc cagcgcgtgc gcgagcagcg cgactacagc gtgcgcgtgc atcacgatgc
     3361 cgatgacgag accggggcac tggtgcgcgg cttcaacgac atgctcgaac agatccgcca
     3421 gcgcgacctc gaactggcgc ggctggccga gcgtctcggc gccgaggtcg agcaccgcac
     3481 gcaggagctc tccggggcca accgcgagct gcagtcgatg gtcggggagc tgcgcagcga
     3541 gaaggagcgg gccgaggcgg cgagcctcgc caagacgcag ttcctcgcca gcatcagcca
     3601 cgagatccgc acgccgatga acggcgtgct cggcatggcc gagctgctgc tgcacaccgc
     3661 gctcgatcat cgccagcgcc acctctcgac catgatccag cgctccgggc gcacgctgct
     3721 cggcatcatc aacgacatcc tcgaccagtc gaagatcgag gccgggcgcc tcgaactcga
     3781 cgccgaggaa ttcgcgctgc gcgagcatct ggagcagacc atcgagctct acgccgagga
     3841 tgcccgccgc cacggcctgg cgtttcgcgc cgactatgcc ccgcacctgc cgcggcgcgt
     3901 gatcggcgat cccgggcggc tcgcgcaggt gctcggcaac ctgctcgcca acgccatcaa
     3961 gttcacgccg cacggctcga tcaccgtgca cgtcgatgcg ctgcccggtg ccggtcccga
     4021 ggtcacgatc ggctgcgagg tcatcgacac cggcatcggc atcgccccgg ccgaccaggc
     4081 gcgcatcttc gaagccttca cgcagaccga cacctccgcc acccgtcgct acggcggcac
     4141 cggcctcggc ctgaccatcg cccgccagat cgtgcagcgc atgggcgggc acatcggcct
     4201 gagcagcgcg cccgatgccg gctcgacctt ctggttcgcc gtgcagctgc gccgcgtgca
     4261 gacgccggcg gcgctggagg agcacgcgct gcgcagcgcc cgcgtgctgc tggtcggcct
     4321 cggcgccgtg
//
LOCUS       DAOQJH010000143         4318 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_12282_length_4318_cov_4.048088, whole genome shotgun sequence.
ACCESSION   DAOQJH010000143 DAOQJH010000000
VERSION     DAOQJH010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 4318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4318
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12282_length_4318_cov_4.048088"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..460)
                     /locus_tag="PLQ18_04535"
     CDS             complement(<1..460)
                     /locus_tag="PLQ18_04535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HNE00953.1"
                     /translation="MTEPNPPAATFTPHPDLPPPAATTGVIGWLRANLFSSPLNALMT
                     LLVAAALLRWIPPILDWLIFSADWSGSSRQDCTGEGACWVFIKQRLGQFIYGFYPEDQ
                     RWRVNLVWVIAVVASWPLFLKRNRLQVPAGLFMLFVFPVIAYGLYTGPLFG"
     gene            complement(463..1650)
                     /locus_tag="PLQ18_04540"
     CDS             complement(463..1650)
                     /locus_tag="PLQ18_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020394997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HNE00954.1"
                     /translation="MTTPLAGAAPARPPFWNDPAVRALVFQALALIAVIALGWYLVHN
                     TQQNLAQRGIASGFGFLNNAAGFGIVQHLIDYSPADSYGRVFWIGLLNTLLVSVLGIV
                     FATVLGFVVGIARLSRNWLVAKIAMVYVEIFRNIPLLLQIFFWYFAVLRTVPNPRNSL
                     AFGDAFFLNIRGLYVPRPVFGDDFVWVLGALALAVVLAVLLGRWARQRQATTGQPFPV
                     FLVSLAILVVLPGVTYFAAGQPLTWDIPKLAGFNFRGGWVVIPELAALLVALTLYTAT
                     FIAETVRAGILSVSHGQTEAAGALGLPNGLTLRLIVIPQAMRVIIPPLTSQYLNLAKN
                     STLAAAIGYPDLVATFAGTVLNQTGQAIETIAITMSVYLALSLGISMLMNWYNKRKAL
                     VER"
     gene            complement(1797..2822)
                     /locus_tag="PLQ18_04545"
     CDS             complement(1797..2822)
                     /locus_tag="PLQ18_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003448888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HNE00955.1"
                     /translation="MRKSSHSAAVVTAALLAFAGTAHAGATLDAVKKKGFVQCGVSDG
                     LPGFSYTDDKGNVSGLDADFCRAVAAAVFGDATKVKFTPLTAKERFTALQSGEIDILS
                     RNTTWTSSRDTALGLNFTGVTYYDGQGFLVNKKLGVKSAKELDGASICIQAGTTTELN
                     LADYFRANNMKFTPISYDKSDESAKAMEAGRCDGLTSDQSQLYAQRIKLAAPDDYIVL
                     PEVISKEPLGPVVRHGDDEWFDIVKWTLYVQVAAEEYGLTSKNVAEAAKTSTNPDVKR
                     LLGAEGEKGKDFGLANDWVVNVVRQVGNYGEMFNRNVGDGSPLKIKRGLNALWNQGGL
                     QYAPPFR"
     gene            3224..>4318
                     /locus_tag="PLQ18_04550"
     CDS             3224..>4318
                     /locus_tag="PLQ18_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017678211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HNE00956.1"
                     /translation="MKPTEEHSGGPRFAVALRETLRAGYGVSDLRADLLAGITVGIVA
                     VPLAMALAIGSGVPPQYGLYTAVIAGLLIALTGGARFSVSGPTAAFIVILHPIAEQYG
                     MGGLVLASLMAGVMLLGMGIGRMGKLIQFIPYPVTTGFTAGIGVVIASLQFKDFFGLS
                     FAHAPESFFERMHQVGLALPGLNPADLLVGAVTLAVLILWPKLRTPIPQHLVALAVGT
                     LLAYGLERLFHVEVATIASRFSYTLDGETLHGIPALLPQFVLPWNLPGADGAPLGLTL
                     ATVKSLVGPAMAIALLGAIESLLCAVVADGMIDDRHDSNQELMAQGIANIGSGLLGGI
                     PATGAIARTAANVRNGARTPVAGIIHAVVLL"
BASE COUNT          683 a         1423 c         1547 g          665 t
ORIGIN      
        1 ggccgaacag cgggccggtg tacaggccgt aggcgatcac cgggaacacg aacagcatga
       61 acagcccggc cggcacctgc aggcggttgc gcttcaggaa cagcggccac gaggcgacca
      121 cggcgatgac ccagaccagg ttcacgcgcc agcgctggtc ctcggggtag aagccgtaga
      181 tgaactggcc gaggcgctgc ttgatgaaca cccagcaggc gccctcgccc gtgcagtcct
      241 gacggctgct gccgctccag tcggcgctga agatcagcca gtcgaggatc ggcgggatcc
      301 agcgcagcag cgccgccgcg accagcagcg tcatcagcgc gttcagcggc gacgagaaca
      361 ggttggcgcg cagccagccg atcacgccgg tggtggcggc cggcggcggc aggtcgggat
      421 gcggcgtgaa ggtggccgcg ggtggattcg gctcggtcat gatcaacgct ccaccagcgc
      481 cttgcgcttg ttgtaccagt tcatcagcat cgaaatgccc agcgacagcg ccagatacac
      541 cgacatcgtg atcgcgatgg tctcgatcgc ctgaccggtc tggttcagca ccgtgccggc
      601 gaaggtcgcg accaggtcgg ggtagccgat cgccgcggcc agcgtcgagt tcttggcaag
      661 gttcaggtac tggctggtca gcggcgggat gatcacgcgc atcgcctgcg ggatcacgat
      721 caggcgcagc gtcaggccgt tcggcagccc gagcgcgccg gcggcttcgg tctggccgtg
      781 gctgaccgac aggatgccgg cacgcacggt ctcggcgatg aaggtcgccg tatacagcgt
      841 cagcgccacc agcagcgccg cgagttccgg aatcaccacc cagccgccgc ggaagttgaa
      901 gccggcgagc ttggggatgt cccaggtgag cggctggccg gctgcgaagt acgtcacgcc
      961 gggcagcacg acgaggatcg cgagcgacac caggaacacc gggaagggct ggccggtcgt
     1021 tgcctggcgc tgccgcgccc agcgccccag cagcacggcc agcacgacgg ccagcgccag
     1081 cgcgccgagc acccagacga aatcgtcacc gaacaccggg cgcggcacgt acaggccgcg
     1141 gatgttcagg aagaaggcat ccccgaacgc cagcgagttg cgcgggttcg gcaccgtgcg
     1201 cagcaccgcg aagtaccaga agaagatctg cagcagcagc gggatgttgc ggaagatctc
     1261 gacgtagacc atcgcgatct tcgcgaccag ccagttgcgc gacagccgcg cgatgccgac
     1321 cacgaagccg agcacggtcg cgaacacgat gccgagcacc gagaccagca gcgtgttcag
     1381 cagtccgatc cagaacacgc ggccgtagct gtcggccggc gagtagtcga tcaggtgctg
     1441 gacgatgccg aagccggcgg cgttgttcag gaagccgaag cccgaggcga tgccgcgctg
     1501 ggcgaggttc tgctgcgtgt tgtggaccag ataccagccg agggcgatga cggcgatgag
     1561 cgcgagcgcc tggaagacca gggcccggac cgcgggatcg ttccagaacg gcggccgggc
     1621 aggtgctgcg cctgcgaggg gcgtagtcat gggaggcgct ccggacggcg gcgcgatcgg
     1681 gggatcgcgt caccgcgcac ggcccggggt ccggcgcatc cggcgccgcc gcgggctcgg
     1741 gtcggtgcgg cggcgcgagt ccggcgccgc cggtgaggtc atgcggccgg cgcggctcag
     1801 cggaagggcg gtgcgtactg caggccgccc tggttccaca gggcgttcag gccgcgcttg
     1861 atcttcagcg ggctgccgtc accgacgttg cggttgaaca tctcgccgta gttgccgacc
     1921 tgcctgacga cgttgaccac ccagtcgttg gccagcccga agtccttgcc cttctcgccc
     1981 tcggcgccga gcaggcgctt cacgtcgggg ttggtcgagg tcttggcggc ctcggcgacg
     2041 ttcttggagg tcaggccgta ttcctcggcg gcgacctgca cgtacagcgt ccacttgacg
     2101 atgtcgaacc actcgtcgtc gccgtggcgc acgaccgggc cgagcggctc cttggagatg
     2161 acctcgggca gcacgatgta gtcatcgggc gcggcgagct tgatgcgctg cgcgtaaagc
     2221 tgcgactggt cggaggtcag gccgtcgcag cggccggctt ccatcgcctt cgcgctctcg
     2281 tcggacttgt cgtagctgat cggcgtgaac ttcatgttgt tggcgcggaa gtagtcggcg
     2341 aggttcagct ccgtcgtcgt tccggcctgg atgcagatcg aggcgccgtc gagctccttc
     2401 gcgctcttga cgccgagctt cttgttcacg aggaagccct ggccgtcgta atacgtgacg
     2461 ccggtgaagt tcaggccgag cgcggtgtcg cgcgaggaag tccaggtcgt gttgcgcgac
     2521 aggatgtcga tctcgccgga ctgcagcgcc gtgaagcgct ccttggccgt cagcggcgtg
     2581 aacttgacct tggtcgcatc gccgaacacc gccgccgcca ccgcgcggca gaagtcggca
     2641 tcgagaccgc tgacgttgcc cttgtcgtcg gtatacgaga agcccggcag gccgtcgctg
     2701 acgccgcact gcacgaagcc tttcttcttg accgcatcga gcgtggcacc ggcgtgggcg
     2761 gtgccggcaa aggcgagcag ggcggccgtg acgacggcgg cactgtggct ggacttgcgc
     2821 atgaattacc tccgggggtg atgggagtcg ctgccgcggt cggtccgcgt caagatcgat
     2881 tgacagggaa ggaccaatcg tgcgcgctgc ttcgtgtgca actcgtcgca gcatcgggcg
     2941 gcgagccggt ctggagtcta gccacgccct gcgtcatcag cagcgcagca ttgccggttt
     3001 cggcaagggt tcgtgacagt ggccgcttgc ctgccgccaa agcaatcacc gcgccagaag
     3061 cgcggcagaa ccggaaaacg gccgccaaac cgtggatcag ccggtccttt cccgcgtcgg
     3121 ccggggcatg gtgcgtgcta gcatcgcccg gtcatcgccg ccccgggggt gggcggctgc
     3181 accagatcgg ccgcgggcga gcggcacacc gacaaggcag aacatgaaac cgacggaaga
     3241 acactcggga gggccgcgct ttgccgtggc gctgcgcgag acgctgcgtg ccggctacgg
     3301 cgtgagcgac ctgcgcgccg acctgctggc cggcatcacg gtcggcatcg tcgccgtacc
     3361 gctggcgatg gcgctggcga tcggcagcgg cgtgccgccg cagtacggtc tgtacacggc
     3421 ggtgatcgcc ggcctgctga tcgcgctgac cggcggcgcc cgcttcagcg tctccggccc
     3481 gaccgcggcc ttcatcgtca tcctgcaccc gatcgccgag cagtacggca tgggcgggct
     3541 cgtgctcgcg agcctgatgg ccggcgtgat gctgctcggc atgggcatcg ggcgcatggg
     3601 caagctgatc cagttcatcc cctacccggt cacgaccggc ttcacggccg gcatcggcgt
     3661 cgtgatcgcg tcgctgcagt tcaaggattt cttcggcctg agcttcgcac acgcgcccga
     3721 gagcttcttc gagcgcatgc atcaggtggg gctggcgctg cccggcctca atccggccga
     3781 cctgctggtc ggcgcggtga cgctcgcggt gctgatcctg tggccgaagc tgcgcacgcc
     3841 gatcccgcag cacctcgtcg cgctcgccgt cggcacgctg ctcgcgtacg gactcgaacg
     3901 gctgttccac gtcgaggtcg cgaccatcgc ctcgcgcttc tcgtacacgc tcgatggcga
     3961 aaccctgcac ggcattccgg cgctgctgcc gcagttcgtg ctgccgtgga acctgccggg
     4021 cgcggacggg gcgccgctcg ggctgacgct ggcgacggtc aagtcgctgg tcggaccggc
     4081 gatggcgatc gcgctgctcg gggcgatcga atcgctgctc tgcgcggtgg tggccgacgg
     4141 catgatcgac gaccgccacg attcgaacca ggaactgatg gcgcagggga tcgccaacat
     4201 cgggtccggc ctgctgggcg ggatcccggc caccggcgcg atcgcgcgga cggcggcgaa
     4261 cgtgcgcaac ggggcgcgga ccccggtcgc gggcatcatc catgccgtgg tcctgctg
//
LOCUS       DAOQJH010000144         4314 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_12300_length_4314_cov_4.404320, whole genome shotgun sequence.
ACCESSION   DAOQJH010000144 DAOQJH010000000
VERSION     DAOQJH010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 4314)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4314)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4314
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12300_length_4314_cov_4.404320"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..692
                     /locus_tag="PLQ18_04555"
     CDS             <1..692
                     /locus_tag="PLQ18_04555"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNE00957.1"
                     /translation="LEAQAAVVAAKAQVRTAELNLGYTTIRSPLTGKAGDSAQREGSL
                     VTPGSAGLLTTLVQTDPMWVVFGLGEQDVLRFRSGVAAGTLKTPGQGKVAAEIVLADG
                     RVFPQQGVITYVAPTVDAQTGTVTLRAEVPNPDGSLSPGQFVRTRAVNIELVDALMVP
                     QRAVMQGAKGKFVYVVVDGKAEVRPVEPGQFYGDQWIITKGLKAGDPVVVDGAIKLAP
                     GAPVKVTGPAK"
     gene            709..3909
                     /locus_tag="PLQ18_04560"
     CDS             709..3909
                     /locus_tag="PLQ18_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018424536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HNE00958.1"
                     /translation="MISRFFIDRPVFAGVLSIVIVIAGLAAMSGLPIAQFPEITPPLL
                     QITASYPGADAETASGTVAAPIEQQVNGVDNMIYMQSNNSNSGDTGLNVYFDIGTDVD
                     RVLTDLTNRANLANPQLPQDVTRNGVVIKKSSPNILMAITILSDGSRDPNEVANYTSI
                     NIVDELKRIPGANQVSMFGLTDYAMRIWLRPDRMAELGITTADVRKAIQEQNTQPAAG
                     RIGQEPTAGPVQLSFPVTARGRLSTPEEFDDIMVRSNPDGSSIRIRDIARSELGIRSY
                     DVSSQLNGKDATVILVYQQPGANALTVATDIRAALDRLSKNFPSGVSYVVPYDTTKFV
                     EVSIEAVIHTFFEAVVLVVVVVFLFLQNWRATVIPLLAVPVSIIGTFAGMYALDFSIN
                     TLTLFGMILAIGIVVDDAIVVVESVEHNMAQGMSPKEAARRTMDEVSGPVVAIVLVLC
                     AVFVPVGFLGGITGQLYKQFAITIAISVVISGIVALTLSPALAAVLLKPSHGEKNIFF
                     RGFEAGFHKITGGYLLGATFLIKRALLGTVLFAGVLAIAYGLFRTVPTSFVPDEDQGY
                     LIVAAFLPDGASLERTREVAEQIAKEAQQNPAVENVLALGGFNMLDSQNKPNAATVFV
                     PLKDWKERKDPAQYADAVRKQLQQKFAASIGDAMVLVFNPPPIPGLGTTGGFEFWVQN
                     RGEGGTAALEKAVHELIAKSKEEPALAGLSTLIRANSQQLFVDLDRDRARSLGVQVTD
                     VFDLLQGLFGTVYVNDFNKFGRTYRVILQAEPDYRTRPEDINKVYVRASSGQMVPLSA
                     LVQLRYTTGPDNVARFNGFVAARINGGPAPGYSSGQALAAMEKLAREHLPPNMYFEWA
                     GQAFQEKKAGSSSALAFGFGLVMVFLILAAQYEKWTLPLSVLVAVPFGVCGALIAVFA
                     RGLNNDVYFQIGLVTLIGLAAKNAILIVEFAVEYRHAGRSAMDAALEAARLRFRPIVM
                     TSLAFILGCVPLAISSGAGANSRISIGTGVIGGMLGATVLAIFFVPLFYRLFDRGEGQ
                     GHGHAEAPSGVVPDVHALPPDPSAPLPPAPEGDRHA"
     gene            3902..>4314
                     /locus_tag="PLQ18_04565"
     CDS             3902..>4314
                     /locus_tag="PLQ18_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015723940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNE00959.1"
                     /translation="MRKATHPIPSASARLRLSVLAVCAALAAGCAAVGPDYERPPVEV
                     PGQWRLPQPSVNELANGAWWQQFADPALDALIQAALDENRDLRIAASRVEEYRGRYGA
                     TRAEQFPQVSASASGGRARLAESQRLPGQPATGTT"
BASE COUNT          656 a         1515 c         1462 g          681 t
ORIGIN      
        1 atctcgaagc acaggccgcg gtcgtcgccg cgaaggcgca ggtgcgcacg gcggagctga
       61 acctcggcta cacgacgatc cgctcgccgc tgaccggcaa ggccggcgat tccgcgcagc
      121 gcgagggttc gctggtgacg ccgggctcgg cgggcctgct gacgacgctg gtgcagaccg
      181 atccgatgtg ggtggtgttc gggctcggcg agcaggacgt gctgcgcttt cgcagcggcg
      241 tggccgccgg cacgctgaag acgccggggc agggcaaggt tgccgccgag atcgtgctcg
      301 ccgacgggcg cgtgttcccg cagcagggcg tgatcaccta cgtcgcgccg accgtcgatg
      361 cgcagaccgg caccgtgacg ctgcgcgccg aggtgccgaa ccccgacggc tcgctgagcc
      421 cggggcagtt cgtgcgcacg cgcgcggtca acatcgaact cgtcgatgcg ctgatggtgc
      481 cgcagcgcgc cgtgatgcag ggcgcgaagg gcaagttcgt gtacgtggtc gtcgacggca
      541 aggccgaggt gcgcccggtc gagccggggc agttctatgg ggaccagtgg atcatcacca
      601 agggcctcaa ggccggcgat ccggtcgtcg tcgacggtgc gatcaagctc gcaccgggcg
      661 cgccggtgaa agtcaccggc ccggccaagt gagcggaggc gccccgatat gatttcgcgc
      721 ttcttcatcg accgcccggt gttcgccggc gtgctgtcga tcgtcatcgt catcgccggc
      781 ctcgcggcga tgagcggcct gccgatcgcg cagttcccgg agatcacgcc gccgctgctg
      841 cagatcaccg cgtcctaccc cggcgccgac gccgaaaccg cctccggcac ggtcgccgcg
      901 ccgatcgagc agcaggtcaa cggcgtcgac aacatgatct acatgcagtc gaacaactcg
      961 aacagcggtg acaccggcct gaacgtgtac ttcgacatcg gcaccgatgt cgatcgcgtg
     1021 ctgaccgacc tcaccaaccg cgccaacctc gccaatccgc agctgccgca ggacgtgacg
     1081 cgcaacggcg tcgtgatcaa gaagtcctcg ccgaacatcc tgatggcgat cacgatcctg
     1141 tccgacggct cgcgcgaccc gaacgaggtc gccaactaca catcgatcaa catcgtcgac
     1201 gagctcaagc gcatccccgg cgccaaccag gtgtcgatgt tcggcctcac cgactacgcg
     1261 atgcgcatct ggctgcggcc cgaccgcatg gccgagctcg gcatcaccac cgccgacgtg
     1321 cgcaaggcca tccaggagca gaacacgcag ccggcggccg ggcgcatcgg gcaggagccg
     1381 accgccgggc cggtgcagct gtcgttcccg gtcacggcgc gcgggcgcct ctccacgccc
     1441 gaggagttcg acgacatcat ggtgcgctcg aacccggacg gctcctcgat ccgcatccgc
     1501 gacatcgccc gcagcgaact cggcatccgc agctacgacg tgtcgagcca gttgaacggc
     1561 aaggacgcga ccgtgatcct cgtttaccag cagccgggcg ccaacgcgct gaccgtcgcc
     1621 accgacatcc gcgccgcgct tgaccggctg tcgaagaact tcccgagcgg cgtcagctac
     1681 gtcgtgccct atgacacgac caagttcgtc gaggtgtcga tcgaggcggt catccacacc
     1741 ttcttcgagg cggtcgtgct cgtcgtcgtc gtcgtgttcc tgttcctgca gaactggcgc
     1801 gcgacggtca tcccgctgct ggcggtgccg gtgtcgatca tcggcacctt cgccggcatg
     1861 tatgcgctcg acttctcgat caacacgctg acgctgttcg gcatgatcct cgcgatcggc
     1921 atcgtcgtcg acgacgcgat cgtcgtcgtc gagtcggtcg agcacaacat ggcgcagggc
     1981 atgtcgccga aggaggccgc gcgccggacc atggacgagg tcagcggccc ggtggtcgcc
     2041 atcgtgctgg tgctctgcgc ggtgttcgtg ccggtcggct tcctcggcgg catcactggc
     2101 cagctctaca agcagttcgc gatcacgatc gcgatctcgg tcgtgatctc cggcatcgtg
     2161 gcgctgacgc tgtcgccggc cctggccgcg gtgctgctga agccctcgca cggcgagaag
     2221 aacatcttct tccgcggctt cgaggcgggc ttccacaaga tcaccggcgg ctacctgctc
     2281 ggcgcgacct tcctgatcaa gcgcgcgctg ctcggcacgg tgctgttcgc cggcgtgctc
     2341 gcgatcgcgt acgggctgtt ccgcacggtg ccgaccagct tcgtgcccga tgaggatcag
     2401 ggctatctga tcgtcgccgc cttcctgccc gatggtgcca gcctcgaacg cacgcgcgag
     2461 gtggccgagc agatcgcgaa ggaggcgcag cagaacccgg cggtcgagaa cgtgctggcg
     2521 ctcggcggct tcaacatgct cgactcgcag aacaagccga acgccgcgac cgtgttcgtg
     2581 ccgctgaagg actggaagga acgcaaggac ccggcgcagt acgccgatgc ggtgcgcaag
     2641 cagctgcagc agaagttcgc cgccagcatc ggcgatgcga tggtgctggt gttcaatccg
     2701 ccgccgattc ccggcctcgg caccaccggc ggtttcgagt tctgggtgca gaaccgcggc
     2761 gagggcggca cggcggcgct cgagaaggcc gtgcatgagc tgatcgcgaa atccaaggag
     2821 gaaccggcgc tcgccggcct gagcacgctg atccgcgcca actcgcagca gctgttcgtc
     2881 gatctcgacc gcgaccgcgc gcgctcgctc ggcgtgcagg tcaccgatgt gttcgacctg
     2941 ctgcaggggc tgttcggtac cgtctacgtc aacgacttca acaagttcgg ccgtacctac
     3001 cgcgtgatcc tgcaggccga gccggactac cgcacgcggc ccgaggacat caacaaggtc
     3061 tacgtgcgcg cgtcgagcgg ccagatggtg ccgttgtcgg cgctggtgca gctgcgctac
     3121 acgaccggcc cggacaacgt cgcgcgcttc aacggcttcg tcgccgcgcg catcaacggc
     3181 gggccggcac cgggctattc gtccggtcag gcgctggcgg cgatggagaa gctcgcgcgc
     3241 gagcacctgc cgccgaacat gtacttcgag tgggccggcc aggccttcca ggagaagaag
     3301 gccggctcgt cgtcggcgct cgccttcggc ttcggtctgg tcatggtgtt cctgatcctc
     3361 gcggcgcagt acgagaaatg gacgctgccg ctcagcgtgc tggtggcggt gcccttcggc
     3421 gtctgcggcg cgctgatcgc ggtcttcgcg cgcggcctca acaacgacgt gtacttccag
     3481 atcggcctgg tgacgctgat cggtctggcg gcgaagaacg ccatcctgat cgtcgagttc
     3541 gccgtcgagt accgccacgc cgggcgctcg gcgatggatg ccgcgctcga ggccgcacgg
     3601 ctgcgcttcc ggccgatcgt gatgacctcg ctcgcgttca tcctcggctg cgtgccgctc
     3661 gcgatcagca gcggtgccgg cgccaacagc cgcatctcga tcggcaccgg cgtcatcggc
     3721 ggcatgctcg gcgcgaccgt gctcgcgatc ttcttcgtgc cgctgttcta ccggctgttc
     3781 gaccggggtg aaggccaggg ccacggccac gccgaagcgc cgtccggggt cgtgcccgac
     3841 gtgcacgcgc tgcctcccga tccgtccgca ccgctgccgc ccgccccgga gggcgaccgc
     3901 catgcgtaag gccacgcacc cgatcccgtc tgcttccgcg cgcctgcgcc tgtccgtgct
     3961 cgccgtctgt gcggcgctcg cggccggctg cgcggcggtc ggtcccgact acgaacgtcc
     4021 gccggtcgag gtgccgggcc agtggcggct gccgcagccg agcgtcaacg agctggccaa
     4081 cggcgcctgg tggcagcagt tcgccgatcc ggcgctcgat gcgctgatcc aggccgcgct
     4141 cgacgagaac cgcgacctgc gcatcgccgc gtcgcgcgtc gaggagtacc gcggacgcta
     4201 cggtgcgacc cgtgccgagc agttcccgca ggtctcggcg agcgcctcgg gcggccgggc
     4261 gcggctcgcc gagagccagc gcctgcccgg ccagccggcg accggcacga cctg
//
LOCUS       DAOQJH010000145         4311 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_12315_length_4311_cov_3.974859, whole genome shotgun sequence.
ACCESSION   DAOQJH010000145 DAOQJH010000000
VERSION     DAOQJH010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 4311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4311
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12315_length_4311_cov_3.974859"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2698
                     /locus_tag="PLQ18_04570"
     CDS             <1..2698
                     /locus_tag="PLQ18_04570"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE00960.1"
                     /translation="PAPLPPPLPDWVEALLLVLDDCRAGSTTARDALIERLAVIVAPL
                     ASAEAVRVARALEDYLQALCRHGRGLDAADILLCEDCASALQAYALGETIDPGALGEL
                     LNALARRAAALPSFAAPLPAEPQPVLPAIDPELAAVFLAEAADILDAADVTLSRWRQD
                     RHSAELLNDLRREMHTLKGSSRMAGFPQIGDLAHATESLLDACAGGKSEPTDALADGM
                     QRALDHFSTMLTEVEANRAPAPAAELIHDLADLLEHGAPVPAPAPAAATAATTTTAKP
                     ASPAGTAESVRVSSTLLDNLVNQMGESSIYRARIEQGVSAVRFNLGELDQIANRLRAQ
                     LRRLEIETEAQILFRYERGGVDPNADFDPLELDRFSELQQLSRQLLEAAEDLANIQSS
                     LVDQTGEIGGLLEQQGKVNKELQQGLMQTRVVRFETVAARLKRVVRQAADDAGKSAEL
                     HIDGGDAEIERNLLEGLVAPLEHLLRNAVSHGIESPERRHAAGKDATGAIRLALRREG
                     AEILLSVADDGAGLNYAAIRASAEKRGLLAANAQVSDAQLADLLLLPGFSTAQALTQL
                     SGRGVGLDVVASAVSSMRGALSIQSQPGKGVTFQLRLPFSLSLTQALLVRAGEDIYAV
                     PLLSVEAVSRLSPREFRDYLAGHTAEHDYAGSGFAVHSLAAVFGGALPAAGSSEARPP
                     VLLFRSADMRAAIQVDAVLGRQEIIVKPVGPQLSAVPGVSGATVLADGRVVIILDMPA
                     LVRGLASAARRAQEAAALRAARQADERAPLSILVVDDSITMRKVTARVLERHGVVVEL
                     AKDGVDAVEVLDQLVPDLVILDIEMPRMDGFELVGHIRNQARLRHLPVIMVTSRTGEK
                     HRDRAARLGVNAYLGKPYQEEELLGSIRDVLGSEGARRLRSL"
     gene            2871..3329
                     /locus_tag="PLQ18_04575"
     CDS             2871..3329
                     /locus_tag="PLQ18_04575"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HNE00961.1"
                     /translation="MPDRELRCLLIGFAGGRLLLPGSMVVEVLPYATPLRVAEVPRWV
                     LGSLLWRARTVPLLAIEPLVTGQPAAPGPRSRIVVLTTPSERVQHLALVSLEVPQLSL
                     LKREMLKASDEVDCPPGMLRAVRIGGDQDAFIPDFDLLCEELAPLGIYRA"
     gene            complement(3359..4048)
                     /locus_tag="PLQ18_04580"
     CDS             complement(3359..4048)
                     /locus_tag="PLQ18_04580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE00962.1"
                     /translation="MNLRVRALALPSLALLLSACTTYVPVPYRPMADAGVSPGACGNF
                     EADSRYAPLRGRVALDNPAQQTETMLRDPGRPSRAERAALGEWRRASDQCRRTTIAAQ
                     RDAGVPEAQLALDTRLADDLDALSGQLAAGRISYGEFARQRQSLADGYNSASARVAGS
                     GEGYAPRYYAAPPVYYSAPAPVYVAPPAYYYPPPYYYRPWYPAIGGSVWYGSGWHGRH
                     GHGGWGVGIGF"
     gene            4182..>4311
                     /locus_tag="PLQ18_04585"
     CDS             4182..>4311
                     /locus_tag="PLQ18_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008173265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HNE00963.1"
                     /translation="MTLDTSWFSDVFADAPSAFSLKVTRKLHEEQSPFQKVEVYETE"
BASE COUNT          587 a         1598 c         1522 g          604 t
ORIGIN      
        1 acccgcaccg ctgccgccgc cgctgccgga ctgggtcgag gcgctgctgc tggtgctcga
       61 cgactgccgt gccggcagca cgaccgcccg cgatgcgctg atcgaacggc tggccgtcat
      121 cgtggcgccg ctcgcctcgg ccgaggccgt gcgcgtcgcc cgcgcgctcg aggactatct
      181 gcaggcgctc tgccgccacg gtcgcggcct cgatgccgcc gacatcctgc tctgcgagga
      241 ctgcgcctcg gcgctgcagg cctacgcgct cggcgagacc atcgaccccg gcgcactcgg
      301 cgagctgctg aacgcgctgg cgcgccgggc ggcggcgctg ccgtcgttcg ccgcaccgct
      361 gccggccgag ccgcaaccgg tgctgccggc catcgatccg gagctggccg cggtattcct
      421 cgccgaggcg gccgacatcc tcgatgccgc cgacgtgacg ctgtcgcgct ggcggcagga
      481 tcgtcacagc gccgagctgc tgaacgacct gcgccgcgag atgcacacgc tcaagggcag
      541 ctcgcgcatg gccggcttcc cgcagatcgg cgacctcgcg catgcgaccg aatcgctgct
      601 cgatgcctgc gccggcggca agtccgaacc gaccgatgca ctcgccgacg gcatgcagcg
      661 cgcgctcgat cacttcagca cgatgctgac cgaggttgag gccaatcgcg ccccggcccc
      721 ggcggccgag ctgatccacg atctggccga cctgctcgaa cacggcgccc cggtgccggc
      781 accggcgccc gccgcggcga cggccgcaac caccacgact gccaaaccgg cgtccccggc
      841 cggcactgcc gaatcggtgc gcgtctccag cacgctgctc gacaacctcg tcaaccagat
      901 gggcgagagc agcatctacc gtgcgcgcat cgagcagggc gtctcggccg tgcgcttcaa
      961 cctcggcgag ctcgaccaga tcgccaaccg cctgcgcgcg cagctgcgcc ggctggagat
     1021 cgagaccgag gcgcagatcc tgttccgcta cgagcgcggc ggcgtcgatc cgaacgccga
     1081 cttcgatccg ctcgaactcg accgcttctc ggaactccag cagctgtcgc gccagctgct
     1141 ggaggcggcc gaggacctcg ccaacatcca gtcgtcgctg gtcgaccaga ccggcgagat
     1201 cggcggcctg ctcgaacagc agggcaaggt caacaaggag ctgcagcagg gtctgatgca
     1261 gacccgcgtc gtgcgcttcg agacggtcgc ggcgcgcctc aagcgcgtcg tgcgccaggc
     1321 ggccgacgat gccggcaagt ccgccgagct gcacatcgac ggcggcgatg ccgaaatcga
     1381 gcgcaacctg ctcgaaggtc tggtcgcgcc gctcgaacac ctgctgcgca acgccgtgtc
     1441 gcacggcatc gagtcgcccg agcgccggca cgcggccggc aaggacgcga ccggtgccat
     1501 ccggctggcg ctgcgccgcg agggcgccga gatcctgctg tcggtggccg acgacggcgc
     1561 cggcctcaac tacgcggcca tccgcgcctc ggccgagaag cgcggcctgc tcgccgccaa
     1621 tgcgcaggtg tccgatgcgc agctcgccga cctgctgctg ctgccgggct tctcgaccgc
     1681 gcaggcgctg acccagctct cgggccgcgg cgtcggcctc gacgtggtcg cgagcgcggt
     1741 gtcgtcgatg cgcggcgcgc tgtcgatcca gtcgcagccg ggcaagggcg tcaccttcca
     1801 gctgcgcctg ccgttctcgc tgtcgctgac gcaggcgctg ctggtgcgcg ccggcgagga
     1861 catctacgcg gtgccgctgc tgtcggtcga ggcggtgagc cgcctcagtc cgcgcgagtt
     1921 ccgcgactac ctcgccgggc acaccgccga gcacgactac gccggttccg gcttcgcggt
     1981 gcacagcctc gcggccgtgt tcggcggcgc cctgccggcg gccggcagca gcgaggcgcg
     2041 cccgccggtg ctgctgttcc gctcggcgga catgcgcgcg gcgatccagg tcgatgcggt
     2101 gctgggccgc caggaaatca tcgtcaagcc ggtcggcccg cagctgtcgg cagtgcccgg
     2161 cgtctcgggc gcgaccgtgc tcgccgacgg ccgcgtcgtc atcatcctcg acatgccggc
     2221 gctggtgcgc ggtctcgcct cggccgcccg ccgggcgcag gaagccgcgg cgctgcgcgc
     2281 ggcgcgtcag gccgacgagc gtgcgccgct gtcgatcctg gtcgtcgatg actcgatcac
     2341 gatgcgcaag gtcacggccc gcgtgctgga gcgccacggc gtcgtcgtcg aactggcgaa
     2401 ggacggcgtc gatgcggtcg aggtgctcga tcagctggtg ccggatctgg tcatcctcga
     2461 catcgagatg ccgcgcatgg acggcttcga gctggtcggc cacatccgca accaggcgcg
     2521 gctgcgccac ctgccggtga tcatggtcac ctcgcgcacc ggcgagaagc accgcgaccg
     2581 cgccgcgcgg ctcggcgtca acgcctacct cggcaagccc tatcaggaag aggaactgct
     2641 cggctcgatc cgcgacgtgc tcggcagcga gggcgcgcgc cggctgcgca gcctgtgatg
     2701 ccgctcacga gtgaatgcgt tgttgatccc cctctctccc cggccccgtc cccgcgaggg
     2761 gaccggggct gaaaagaccg ggcgttcggc tgtgaccggc atgagcgaag aacccggccc
     2821 cctgtgagcc ctgatccggg cgccggcggc gcctgcacgg agactgcgtg atgcccgatc
     2881 gcgaactgcg ctgcctgctc atcggcttcg ccggcggacg cctgctgctg cccggctcga
     2941 tggtcgtcga ggtactgccg tacgcgaccc cgctgcgcgt tgccgaagtg ccgcgctggg
     3001 tgctcggctc gctgctctgg cgcgcgcgca ccgtgccgct gctcgcgatc gagccgctgg
     3061 tcaccggcca gccggccgcg cccggcccgc gctcgcgcat cgtcgtgctg accacgccga
     3121 gcgagcgcgt gcagcacctc gcgctggtgt cgctcgaagt gccgcagctg tcgctgctca
     3181 agcgcgagat gctgaaggcc agcgacgagg tcgactgccc gcccggcatg ctgcgtgccg
     3241 tgcgcatcgg cggcgatcag gatgccttca tccccgactt cgacctgctg tgcgaggaac
     3301 tcgcgccgct cggcatctac cgcgcctgag cggagcctcc ggcaccggga cccggcactc
     3361 agaagccgat cccgacgccc cagccgccgt ggccgtggcg gccgtgccag ccgctgccgt
     3421 accagacgct gccgccgatc gccggatacc agggccggta gtagtacggc ggcgggtagt
     3481 aatacgccgg cggcgccacg tagaccggcg ccggcgcgct gtaatagacc ggcggggccg
     3541 cgtagtagcg cggcgcatag ccttcgccgc tgccggcgac gcgcgcactg gcgctgttgt
     3601 agccatcggc gagcgactgg cgctggcgcg cgaactcgcc gtagctgatg cgcccggcgg
     3661 cgagctggcc gctcagcgcg tcgaggtcat cggcgagccg ggtgtcgagc gcgagctgcg
     3721 cctcgggcac gccggcatcg cgctgcgcgg cgatcgtcgt gcgccggcac tgatcgctcg
     3781 cgcgtcgcca ctcgccgagc gcggcacgct ccgcgcggct cggccggccc ggatcgcgca
     3841 gcatcgtctc ggtctgctgc gccggattgt cgagtgcgac ccggccgcgc agcggcgcgt
     3901 agcggctgtc ggcctcgaag ttgccacagg cgccgggcga aaccccggca tcggccatcg
     3961 gccggtacgg caccggcacg taggtggtgc aggcggacag cagcagcgcc agcgacggca
     4021 gcgcgagcgc gcgaacgcga aggttcatgg cggcattcct cttcgacggg tggaccggtc
     4081 ccgcggcgga tggtcatgca cggaggctag aatgcgcgcc cccgatcctc atcctgatcc
     4141 ccgatagtgc gccggcctcg ccgggcagga gttaagactg catgacgctc gacacgagct
     4201 ggttctcgga cgtcttcgcc gatgccccgt cggcgttctc gctgaaggtc acccgcaagc
     4261 tgcacgagga gcagagcccg ttccagaagg tcgaggtgta cgagaccgag g
//
LOCUS       DAOQJH010000146         4309 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_12326_length_4309_cov_4.610954, whole genome shotgun sequence.
ACCESSION   DAOQJH010000146 DAOQJH010000000
VERSION     DAOQJH010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 4309)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4309)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4309
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12326_length_4309_cov_4.610954"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1378)
                     /locus_tag="PLQ18_04590"
     CDS             complement(<1..1378)
                     /locus_tag="PLQ18_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNE00964.1"
                     /translation="MIESPAAQPPSPGRPAGSGLRAPAGLGVLGLLLAVWHDEWSVPA
                     AAGIVVLALAAGLALRIAALERAARQALAAELDETRRRLDAIIGQSPSALSLKDLDGR
                     YVCVNGNVERLLQRPAAGILGRTDSELYLPDTARVFREHDRQVLETRRRHSVEESVVV
                     DGEPRLYISHMFPVPDAHGEPALVCRIALDITETRRAQDALVASEARLRAIFAGIGDA
                     AIYCDTGRSIELVNPAFTKIFGYPAQEVIGRSTAMLYADAADFEAAGRVCYHPDMDSD
                     PLPRTLRYRRRDGSLFWSESVGMPVRNDRDELIGYLGVHRDVSARLATERALRDSESR
                     LRLFVEYAPAALAMFDRDMRYLVASHRWRQDYGLGERPLVGVSHYEVFPEIPESWREA
                     HRRGLAGEVLGAPADRFVRVDGSEQWIRWEIRPWRDAGGAVAGIVIFADDISALLRAS
                     EEVRRLNAE"
     gene            complement(1509..2225)
                     /locus_tag="PLQ18_04595"
     CDS             complement(1509..2225)
                     /locus_tag="PLQ18_04595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE00965.1"
                     /translation="MNRPAFPFRWQRLPVALLLLVAGCGPQHDPADLFPLERGRAWTY
                     RMQTLNGPDFRRETLRIEALGPRELDGRTVWVRHTSDGTEYYLERDARGLRRVAQRSV
                     LDDLPRWDAPERTVLPAAPAVGQHWSVPGRPYLPHRVRPVVEDLRQTRPFEFEYGIED
                     TDATVEVPAGRFAHCVHVRGEASVYLVADPRFGPQDVPITQDEWYCPGTGLVKLERRE
                     PIEAADVYGGSLTLELEAAR"
     gene            complement(2222..2743)
                     /locus_tag="PLQ18_04600"
     CDS             complement(2222..2743)
                     /locus_tag="PLQ18_04600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE00966.1"
                     /translation="MSAAGWGITGAYVLLALLVGSLWLHARLAVWVKLAAVGLVTASY
                     WLVWTTLPGFAGWPAEDELPARFQLLASEIREPDAANGFPGAVHVWVVDLAGDRPAAA
                     PRAYRLAYGKDLHASLDAAKKRIQEGLPQVGRRTDDVRLGVAIDRSRFGERHDELDRL
                     LIENLPDPRLPEK"
     gene            complement(2740..3351)
                     /locus_tag="PLQ18_04605"
     CDS             complement(2740..3351)
                     /locus_tag="PLQ18_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE00967.1"
                     /translation="MSLRRKRRDFELDPFTDLLFNALLGITFMFLLAFVAMNPKAKTG
                     TIEAKAEFIITVTWPDNSPDDIDTWVESPDGEVVWFKHPDAGLMHLDRDDRGLVGDQI
                     IVDGRVISNPLNEEVVTLRGVVPGEYTVNVQYYESGTRAPVPVEVRVVKVNPHLSVVY
                     YGQLKLEHINSEVTAVRFTVMPDGRVTGINTLAKPLAAVRGLK"
     gene            complement(3359..4093)
                     /locus_tag="PLQ18_04610"
     CDS             complement(3359..4093)
                     /locus_tag="PLQ18_04610"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNE00968.1"
                     /translation="MARSEPPLRLFLQWLLLNGALLFALWLCWTQGLIATYVLSDPTW
                     ISQAILGLFVAACGHCGLRAWWLARELEQVTAIMRRARAEPDARLQREDAGLRLGDTA
                     LPPSLASEYFTAVLGKYARTAPPESLDHALLTDLLADHASGSHEGGWFLCGLLLKLGL
                     LGTVIGFVMMLGSIATTDSFDVATLQRMLLAMGLGMKVALYTTMIGLIASMLLGAQYL
                     ALDRAADRLVGAIVHFAETELARRAG"
     gene            complement(4104..>4309)
                     /locus_tag="PLQ18_04615"
     CDS             complement(4104..>4309)
                     /locus_tag="PLQ18_04615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="ribonucleotide reductase subunit alpha"
                     /protein_id="HNE00969.1"
                     /translation="ADAQSPVWDWVMIGALSGTHGTPPTPARAEEVLQTMVRLIEAGG
                     DLSPFVILDRAERPVRLSPAEPV"
BASE COUNT          675 a         1567 c         1482 g          585 t
ORIGIN      
        1 gttcggcgtt gagccggcgc acctcctccg aggcgcgcag cagcgcgctg atgtcatcgg
       61 caaagatgac gatgccggcg accgcaccgc cggcatcgcg ccacgggcgg atctcccagc
      121 ggatccactg ctcgctgccg tcgacgcgca cgaagcggtc ggccggcgcg ccgagcacct
      181 cgccggccag gccgcggcgg tgggcctcgc gccaggactc ggggatctcc gggaacacct
      241 catagtgcga gaccccgacc agcggccgct cgccgaggcc gtagtcctgc cgccagcggt
      301 ggctcgcgac cagatagcgc atgtcgcgat cgaacatcgc cagcgccgcc ggcgcgtatt
      361 cgacgaacag ccgcaggcgc gattcgctgt cgcgcagcgc ccgctcggtc gccagccgcg
      421 cgctgacatc gcggtgcacg ccgaggtagc cgatcagctc gtcgcggtca ttgcgcaccg
      481 gcatgccgac cgactcgctc cagaagagac tgccgtcgcg ccgccggtag cgcagcgtgc
      541 gcggcagcgg atcgctgtcc atgtccgggt gatagcagac gcgaccggcc gcctcgaagt
      601 cggccgcatc ggcgtagagc atcgccgtgc tgcgcccgat cacctcctgc gccggataac
      661 cgaagatttt cgtgaacgcc gggttgacca gctcgatgct gcgcccggtg tcgcagtaga
      721 tcgccgcatc gccgatgccg gcgaagatcg cgcgcagccg cgcctcgctg gcaacgagcg
      781 cgtcctgcgc gcggcgggtc tcggtgatgt cgagcgcgat gcgacagacc agcgccggct
      841 cgccgtgcgc atccggcacc gggaacatgt gcgatatgta gagccgcggc tcgccgtcga
      901 cgacgacgct ttcctcgacc gaatgccgcc tgcgcgtttc cagcacctgc cggtcgtgct
      961 cccggaacac gcgcgcggta tcgggcagat acagctcgct gtcggtacgc ccgaggatgc
     1021 cggccgccgg gcgctgcagc aggcgctcga cgttgccgtt gacgcagacg tagcggccgt
     1081 cgaggtcctt cagcgacagc gccgacggcg actggccgat gatcgcgtcg agccggcggc
     1141 gggtttcgtc gagttcggcg gcgagcgcct gccgcgcggc ccgctccagc gccgcgatgc
     1201 gcagcgccag cccggcggcg agcgccagca cgacgatccc ggccgctgcg gggacggacc
     1261 actcatcgtg ccagacggcg agcagcaggc cgagcacgcc caggccggcc ggtgcgcgca
     1321 ggccggagcc ggccggccgg cccggcgacg ggggctgtgc agcaggggat tcgatcatcg
     1381 cggactccgg acgcagcctc ggcagcgccc cgggaccgcg tcctccctgc ctcacgaccg
     1441 gggcctcgtc cggatagcac agcccggcgc ccgccacctt gcgcgccgtc aactccgcag
     1501 ccgatcgttc agcgcgcggc ttcgagttcc agcgtcagcg agccgccgta gacatcggcc
     1561 gcctcgatcg gctcgcgccg ttcgagcttc acgaggccgg tgccggggca gtaccactcg
     1621 tcctgcgtga tcggcacgtc ctgcgggccg aagcgcggat cggcgaccag atagacgctg
     1681 gcctcgccgc gcacgtgcac gcagtgggcg aaacgtccgg ccggcacctc gaccgtcgca
     1741 tcggtatcct cgatgccgta ctcgaactcg aacggccgcg tctgccgcag gtcctcgacc
     1801 accgggcgca cgcgatgggg cagatacggc cgccccggca ccgaccagtg ctgaccgacc
     1861 gccggggcag ccggcagcac cgtgcgctcg ggagcatccc agcgcggcag gtcatcgagc
     1921 acgctgcgct gcgcgacgcg acgcaggccg cgcgcatcgc gctccaggta gtactcggtg
     1981 ccgtccgagg tatggcgcac ccagaccgtg cgcccgtcga gttcgcgcgg accgagggct
     2041 tcgatgcgca gcgtctcgcg ccggaagtcc gggccgttca gcgtctgcat gcggtaagtc
     2101 cacgcccgtc cacgctccag cgggaacagg tcggccggat cgtgctgcgg tccgcagccg
     2161 gcgacgagca gcagcagcgc gaccggcagc cgctgccagc gaaacggaaa ggcaggccgg
     2221 ttcatttttc cggcagccgc ggatcgggca ggttctcgat cagcaggcga tcgagttcgt
     2281 cgtgacgttc gccgaagcgc gaacggtcga tcgccacgcc gaggcgcacg tcatcggtac
     2341 gccgcccgac ctgcggcagc ccttcctgga tgcgcttctt cgcggcgtcg agcgaggcgt
     2401 gcagatcctt gccgtaggcg agccggtacg cgcgcggcgc cgccgccggc cggtcgccgg
     2461 cgagatcgac gacccagacg tggaccgcgc ccggaaagcc gttcgcggcg tcgggctcgc
     2521 gaatctcgct cgcgagcagc tggaagcgcg ccggcagttc gtcttcggcc ggccagccgg
     2581 caaaacccgg cagcgtcgtc cagaccagcc agtaactggc cgtgaccagc cccacggccg
     2641 cgagcttgac ccacaccgcc agccgcgcat gcagccacag gctgccgacc agcagcgcca
     2701 gcagcacgta ggcaccggtg atgccccagc cggcggcact cacttcagcc cccggaccgc
     2761 tgccagcggc ttggccagcg tgttgatgcc ggtcacgcgc ccgtccggca tgaccgtgaa
     2821 gcgcaccgcg gtcacctcgc tgttgatgtg ttcgagcttc agctgcccgt agtagacgac
     2881 gctcaagtgc ggattgacct tcacgacgcg cacctcgacc ggcaccggcg cgcgcgtgcc
     2941 cgattcgtag tactggacat tgaccgtgta ttccccgggc acgacgccac gcagcgtcac
     3001 gacttcctcg ttcagcggat tcgagatcac gcgaccgtcg acgatgatct gatcgccgac
     3061 caggccgcgg tcgtcgcgat cgaggtgcat cagcccggca tccggatgct tgaaccagac
     3121 cacctcgccg tccgggctct cgacccaggt gtcgatgtcg tcgggactgt tgtccggcca
     3181 ggtcaccgtg atgatgaact cggccttggc ctcgatcgtg ccggtcttcg ccttcgggtt
     3241 catcgccacg aaggcgagca ggaacatgaa ggtgatgccg agcagcgcgt tgaacagcag
     3301 gtccgtgaac ggatcgagct cgaagtcgcg acgcttgcgg cgcagggaca cgtcgtcatc
     3361 acccggcgcg gcgcgccagt tccgtctcgg cgaaatgcac gatggcgccg accagccggt
     3421 cggcggcgcg atcgagtgcg aggtactggg cgccgagcag catcgacgcg atcaggccga
     3481 tcatcgtcgt gtacagcgcc accttcatgc ccaggcccat cgccagcagc atgcgctgca
     3541 gcgtcgcgac atcgaaggaa tcggtggtgg cgatcgagcc gagcatcatc acgaagccga
     3601 tcacggtgcc gagcaggccg agcttcagca gcagcccgca caggaaccag ccgccttcgt
     3661 ggctgcccga ggcgtgatcg gcgagcaggt cggtcagcag cgcgtgatcg agggattccg
     3721 gcggtgcggt gcgggcgtac ttgccgagca cggccgtgaa gtattcgctc gccagcgacg
     3781 gcggcagcgc cgtatccccg agtcgcaggc cggcatcctc ccgctgcagg cgcgcgtcgg
     3841 gctcggcccg ggcccggcgc atgatcgccg tgacctgctc cagttcgcgc gccagccacc
     3901 acgcgcgcag cccgcagtga ccgcaggcgg cgacgaacag cccgaggatc gcctgcgaga
     3961 tccaggtcgg gtccgacagc acgtaggtcg cgatcagccc ctgcgtccag cacagccaca
     4021 gcgcgaacag cagtgcgccg ttgagcagca gccactgcag gaacaggcgc agcggcggtt
     4081 cggaacgtgc cacggctcag ggctcagacc ggttcggccg gcgacagccg caccggccgc
     4141 tcggcgcgat cgaggatcac gaacggcgac aggtccccgc cggcctcgat cagccgcacc
     4201 atggtctgca gcacttcctc ggcgcgcgcc ggcgtcggcg gcgtgccatg cgtgccggac
     4261 agcgcgccga tcatgaccca gtcccagacc ggcgactgcg catcggcat
//
LOCUS       DAOQJH010000147         4302 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_12352_length_4302_cov_4.135625, whole genome shotgun sequence.
ACCESSION   DAOQJH010000147 DAOQJH010000000
VERSION     DAOQJH010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 4302)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4302)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4302
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12352_length_4302_cov_4.135625"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..648)
                     /gene="eutC"
                     /locus_tag="PLQ18_04620"
     CDS             complement(<1..648)
                     /gene="eutC"
                     /locus_tag="PLQ18_04620"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019950123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008851 - ethanolamine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutC"
                     /protein_id="HNE00970.1"
                     /translation="MTDTPPPPVTPNPWTALRRHTDARIALGRAGLSLPSAAHLAFQL
                     DHARARDAVHLPLERAALHAALAQASGLEVLDLHSAAADRLEYLQRPDLGRRLDEASR
                     ALLAARPVTACDVGFVIADGLSARAVHEHAAALLTALLPRLDGWTPGPLALVEQGRVA
                     IGDEIGAALGARLVVVLIGERPGLSSPDSLGLYLTWSPRPGRSDAERNCISNVRPA"
     gene            923..4210
                     /locus_tag="PLQ18_04625"
     CDS             923..4210
                     /locus_tag="PLQ18_04625"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNE00971.1"
                     /translation="MTPSRRDALRIALIYAAFAAIWILFSDIAAEALAPDRAWLTRLS
                     MYKGWLFVGVTALLLYALIRSTLARVEAAEAERVRLYSELAAQLRTFDTVLSACPDLF
                     FLFDADGRCRYISRNGLDLLGLKAVAAHGRTLFELGLPPAVAASCETARLRALGSRDI
                     VTHEIDLPGAQGALCYEILHCALPDAAGRAGGTLATVRDLSARKHDERRIRQLTELYA
                     ALSETGRAVMRVRSRAELYAQVCRIATDHGGLCFAWIGTLDAAAACVRPQVFSGTAGD
                     YLHQLQVFPAGPDAVGPVAEAMRRGAPSVENDLLAMLGRLGLQRAAVLAGKYGLCSAG
                     VFPLREGGCISGVLTVYATEAGFFTPERIGLLAAMAEEIGHALDGQQLAEERARAQAR
                     LAESESRYRELFEASPQPMWVVEAGTLGFLAVNQAALDAYGHARERFLQMALPDLHAS
                     AGAPSGLPDAADREPGEPARCQGLARHRCADGRPIEVEWVAHALVWSAREAWLMLVTD
                     VTERRRAEAGLRLAAQVFEGSGEAIFIADEQQRIVSLNRACAAITGYPAAELPGQPLE
                     RLLGSDDDPDALAACLQALATGGAWKGELWSRRCDGSPYPQWLSMSAVRDPEGRTTHT
                     VAIFTDISERKAAEARIRHLAQHDFLTGLPNRRLLEDRLQQAVAFAARRSGRVGLLFL
                     DLDRFKIINDTCGHPVGDAMLRELACRLTGVLRESDTLGRSGGDEFIVLLPGLDDPAD
                     AGRVGRKLLAAICEPVQVGEHELYVTGSVGIAVYPEDGHDAETLVRHADTAMFHAKHA
                     GRNQCQYFTPAMNTANDARIWLENALRRVLERGELRLEFQPQVEVASGRTLGAEALLR
                     WRHSERGEIAPADFIAVAEDCGLIVPIGAWVLESACRQAREWLDAGLPPVRLSVNVSA
                     VQFRSPGLGASVAAALAASGLEPGWLELEITEGCLMEDATAARTTLEALRAHGVSIAI
                     DDFGTGYSSLAYLKHFPIQRLKIDRSFVSGLPDDASDAAIARAIVHLAQSLHLETVAE
                     GVETAAQRDFLAGLGCESAQGWLWSRALDPDAFAARLLAEASGAAGGHDPDCACGHCL
                     SRSDHPL"
BASE COUNT          545 a         1535 c         1630 g          592 t
ORIGIN      
        1 ggcggggcgg acgttgctga tgcagttgcg ctcggcgtcg ctgcggccgg ggcggggtga
       61 ccaggtgaga tacaggccga ggctgtcggg ggagctcagg cccgggcgtt cgccgatcag
      121 cacgacgacc aggcgcgcac cgagtgcggc gccgatctcg tcgccgatcg cgacgcgccc
      181 ctgctcgacc agcgccagcg gcccgggcgt ccagccgtcc agtcttggca gcagcgcagt
      241 cagcagcgcc gcggcgtgct cgtgcacggc gcgcgcggag aggccatcgg cgatcacgaa
      301 gcccacgtcg caggccgtga ccggccgggc ggcgagcagc gcgcgcgagg cttcgtcgag
      361 ccggcggccg aggtcggggc gctgcaggta ttcgaggcgg tcggcggcgg ccgagtgcag
      421 gtccaggact tcgagtccgc tcgcctgcgc cagcgccgcg tgcagggcgg cgcgctcgag
      481 cggcagatgc acggcgtcgc gggcgcgggc gtggtcgagc tggaaggcca gatgcgcggc
      541 gctcggcagg ctgaggccgg cgcgcccgag cgcgatgcgc gcatcggtgt gccggcgcag
      601 cgccgtccac gggttgggag tgacgggcgg gggcggggtg tcggtcatcg gcggcgctcc
      661 ggtggcggtg gcggcgtccc ggccggggct tgcggcagcg cgctgcgtgc cggcggcggg
      721 gatcggtcca tcctgagccg ccgggccatc cgggtccacc gaggcgtctg ccgggcgctg
      781 cgcgccggcc ggtcgagccg gatgtcgcaa gctgtggcac ctgcgtatgc ggcaatgcgg
      841 cttggataga ctggccgcgt ccgccgatcg gcacggttcc gtggcgggcc gatcgctccc
      901 gagccgccac cggtctgcgc cgatgactcc gtcccgccgc gatgcgctgc gcatcgcgct
      961 gatctatgcg gccttcgccg ccatctggat cctgttctcc gacatcgccg ccgaggcgct
     1021 ggcgccggac cgggcgtggc tgacgcggct gtcgatgtac aagggctggc tgttcgtcgg
     1081 ggtcacggcg ctgctgctgt acgcgctgat ccgcagcacg ctcgcgcgcg tcgaggctgc
     1141 cgaggccgag cgcgtgcggc tgtattcgga actcgccgcg cagctgcgca ccttcgacac
     1201 cgtgctgtcg gcctgtccgg acctgttctt cctgttcgat gccgacgggc gctgccgcta
     1261 catcagccgc aacggcctcg acctgctcgg actgaaggcc gtcgccgcgc acgggcgcac
     1321 gctgttcgag ctcggcctgc cgccggcggt cgcggccagc tgcgagacgg cacgcctgcg
     1381 cgcgctcggc agccgcgaca tcgtcacgca cgagatcgat ctgccgggtg cgcagggagc
     1441 gctctgctac gagatcctgc actgcgcgct gcccgatgcc gccgggcgcg ccggcggcac
     1501 gctggcgacg gtgcgcgacc tgagcgcgcg caagcacgac gagcgccgca tccgccagct
     1561 caccgagctg tacgcggcgc tgagcgagac cggccgcgcc gtgatgcgcg tgcgcagccg
     1621 ggccgagctg tacgcgcagg tctgccgcat cgccaccgac cacggcggac tctgcttcgc
     1681 gtggatcggc acgctcgatg ccgcggccgc ctgcgtgcgc ccgcaggtgt tcagcggcac
     1741 ggccggcgat tacctgcatc agctgcaggt cttcccggcc ggtccggacg ccgtcggtcc
     1801 ggtcgccgaa gccatgcgcc gcggagcacc gagcgtcgag aacgatctgc tggcgatgct
     1861 cggacgcctc ggcctgcagc gtgcggccgt gctcgccggc aagtacgggc tgtgctcggc
     1921 cggcgtgttc ccgctgcgcg agggcggatg catcagcggc gtgctgaccg tctacgccac
     1981 cgaggccggc ttcttcacgc ccgagcgcat cggcctgctg gcggcgatgg ccgaggagat
     2041 cggccacgcg ctcgatggcc agcagctcgc cgaggagcgc gcgcgcgcgc aggcgcggct
     2101 ggccgagagc gagagccgct accgcgagct gttcgaggcc agcccgcagc cgatgtgggt
     2161 ggtcgaggcc ggcacgctcg gttttctcgc cgtcaaccag gcggcgctcg atgcctacgg
     2221 ccatgcgcgc gagcgcttcc tgcagatggc gctgcccgat ctgcacgcaa gtgccggggc
     2281 gccgtccgga ctgccggatg ccgcggatcg cgaacccggg gagccggcgc gctgtcaggg
     2341 gctcgcgcgg caccggtgcg ccgacggccg gccgatcgag gtcgagtggg tcgcgcacgc
     2401 gctggtctgg agcgcacgcg aggcctggct gatgctcgtg accgacgtga ccgagcgccg
     2461 gcgcgccgag gccgggctgc ggctggccgc gcaggtgttc gagggcagcg gcgaggcgat
     2521 cttcatcgcc gacgaacagc agcgcatcgt gtcgctgaac cgcgcctgcg ccgccatcac
     2581 cggctacccg gcggcggaac tgcccggcca gccgctcgaa cgcctgctcg gcagcgacga
     2641 cgatccggac gccctggctg cctgcctgca ggcgctcgcc accggcggag cctggaaggg
     2701 cgagctctgg agccgccgct gcgacggctc gccgtacccg cagtggctgt cgatgtcggc
     2761 ggtgcgcgat ccggagggcc ggaccacgca taccgtcgcg atcttcaccg acatcagcga
     2821 gcgcaaggcc gccgaggcgc gcatccgcca cctcgcgcag catgacttcc tgaccgggct
     2881 gccgaaccgc cggctgctcg aagaccggct gcagcaggcc gtggcgttcg cggcacggcg
     2941 cagcggacgg gtggggctgc tgttcctcga tctcgaccgc ttcaagatca tcaacgacac
     3001 ctgcggtcac ccggtcggcg atgcgatgct gcgcgaactc gcctgccggc tgaccggggt
     3061 attgcgcgaa tccgacaccc tcgggcgttc ggggggcgat gagttcatcg tgctgctgcc
     3121 cggactcgat gacccggccg atgccgggcg cgtcgggcgc aagctgctgg cggcgatctg
     3181 cgaaccggtg caggtcggcg agcacgagct gtacgtgacc ggcagcgtcg gcatcgccgt
     3241 gtatccggag gacggtcacg acgccgaaac cctggtgcgc cacgccgaca cggcgatgtt
     3301 tcatgccaag cacgccggtc gcaaccagtg ccagtacttc acgccggcga tgaacaccgc
     3361 caacgatgcg cgcatctggc tcgagaacgc gctgcgccgc gtgctcgaac gcggcgagct
     3421 gcgcctcgaa ttccagccgc aggtcgaggt ggcgagcggg cgcacgctcg gtgccgaggc
     3481 gctgctgcgc tggcgtcact ccgagcgcgg cgagatcgcg ccggcggact tcatcgcggt
     3541 ggccgaggac tgcgggctga tcgtgccgat cggcgcatgg gtgctggaat cggcctgccg
     3601 gcaggcgcgg gaatggctcg atgccggcct gccgccggtg cggctgtcgg tgaacgtctc
     3661 ggccgtgcag ttccgctccc cgggcctggg cgcgagcgtc gccgcggcgc tggcggcgag
     3721 cgggctggag ccgggctggc tggagctgga gatcaccgaa ggctgcctga tggaggacgc
     3781 cacggccgcg cgcacgacgc tggaggcgct gcgcgcgcac ggggtcagca tcgccatcga
     3841 cgacttcggc accggctatt cgagccttgc ctacctgaag cacttcccga tccagcgcct
     3901 gaagatcgac cgttccttcg tcagcggcct gcccgacgat gccagcgacg cggcgatcgc
     3961 gcgtgcgatc gtgcatctgg cgcagagcct gcacctcgaa accgtcgccg aaggcgtcga
     4021 gaccgccgcg cagcgcgact tcctcgccgg actcggctgc gagtccgccc agggctggct
     4081 gtggtcacgc gcgctcgacc ccgatgcctt cgccgcccgg ctgctggccg aggccagcgg
     4141 ggccgccggt ggccacgacc cggactgcgc ctgcgggcac tgcctgagcc gcagcgatca
     4201 tccgctctga ggccggacgg cggccgcgag cgcgccccgg gcgcgttcgc ccggtcgcgc
     4261 cgcacgcgct agtcggccac cggcacggcc tgcagcaccg tg
//
LOCUS       DAOQJH010000148         4266 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_12522_length_4266_cov_3.765376, whole genome shotgun sequence.
ACCESSION   DAOQJH010000148 DAOQJH010000000
VERSION     DAOQJH010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 4266)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4266)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4266
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12522_length_4266_cov_3.765376"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..653
                     /gene="rseP"
                     /locus_tag="PLQ18_04630"
     CDS             <1..653
                     /gene="rseP"
                     /locus_tag="PLQ18_04630"
                     /inference="COORDINATES: protein motif:HMM:TIGR00054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HNE00972.1"
                     /translation="GLQDGDRIVSVDDQRVADWQDFATRVRAKPEADLRLQIERDGSP
                     LSVRLRPVRIETAQGAVGQIGAFAAVPQSLADSLRVVVRYGPIDAAVAAVERTWEMSA
                     FTLRMLGRMLTGRASLDNLSGPLTIAQMAGQSASIGLSAFLSFLALVSISLGVLNLLP
                     VPVLDGGHLAWYAIETIKGSPVSEAVQSAGQRVGLAVLLVMMGLAFYNDLARLFGP"
     gene            702..3002
                     /gene="bamA"
                     /locus_tag="PLQ18_04635"
     CDS             702..3002
                     /gene="bamA"
                     /locus_tag="PLQ18_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009725337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HNE00973.1"
                     /translation="MTPILRNFLLSASLAAPACIVQAAEFIVRDIRVEGLQRISAGTV
                     FSYLPVQTGQRVRQEDFPDLIRTLFRTGFFTDVSLQRDGDVLVVNVVERPAIAEVKVT
                     GNKDISTEDLEKSLKTVGLAEGRTFDRALLDKVEQELNRQYYARGKYAVKVDSQVRPL
                     ERNRVAITIDISEGIAARIKQINIVGAKAFDEDDLLDLFQSSTSNWTSLLSKDDQYSK
                     QKLGGDLETLRSYYLDRGYLRFNIDSTQVTITPDKKDIYITINIAEGDRYTVKDVRLA
                     GESPVPLDQLKPLVKLQPGQTFSRATLNESTKAITDRLGDDGYAFANVSAVPQIDDAG
                     KTVSLALNVDAGRRVYVRRINFKGNIKTQDEVLRREMRQTEGSWFATRDVNRSKERLE
                     RLAFVQEVTVETPAVPGTPDQVDLNYSVTERPAGSLSFGIGYGQDAGVLLSASVSQQN
                     FLGTGNALSFTFNNSQINTIYSFSYTNPYFTPDGVSAGIRASYRETDASEANLASYLT
                     NEFETMATFGFPLNETDTVNLAFGISGIKLKTNGDNTPQGIYDYIDEHGERHVGPRFE
                     LSWARDTRNKAVFADRGSYNRLSIESMLGGDDSWYKLGYRHSTYLPLTDSLTLLGHFD
                     FGYGEGFGNTDTLPFYENFYAGGLRTVRGYKTNTLGPRWDDPNPDLRDPKGGALRTAG
                     GVEVLFPAPFFKDNKNLRFSTFFDIGNVYAEVNDFDAGELRYSAGLSMQWITPLGPLV
                     MSVAQPLNKKDGDKTEPFQFTFGVPF"
     gene            3034..3549
                     /locus_tag="PLQ18_04640"
     CDS             3034..3549
                     /locus_tag="PLQ18_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010961671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HNE00974.1"
                     /translation="MKALLRVLLGALALTFALPAVAAELKLGFVNAARVLKSAPQAEA
                     ALNRLEKEFSPRDKALVEAQKALRKLEDRLSKDGATLSDAERRDLERDIISQRRDVKR
                     AQDEFREDFNIRRNEEMAKLQKLIQDAITALAQEERYDLVLNDGAVIFASEAVDITDK
                     VLKRLSAVHGN"
     gene            3570..>4266
                     /locus_tag="PLQ18_04645"
     CDS             3570..>4266
                     /locus_tag="PLQ18_04645"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005959117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HNE00975.1"
                     /translation="MHTLAELAQVAGAQLRGDAATPIDGVGTLAAARPGQIGFLANPK
                     YRQLLSGTQASAVILGAEDAGACPVACLIADNPHLAYARVAALFDPAAGRQQGVHARA
                     CVDAAAEVADDAWIGPMAVIEAGAVIGPRVQVGPGCVVGARTSIGADSRLVANVTVLH
                     DCVLGERVLVHPGAVIGADGFGLANDRGCWVKVPQLGRVVIGNDVEIGVNTAVDRGAL
                     EDTVLADGVKLDNL"
BASE COUNT          739 a         1421 c         1422 g          684 t
ORIGIN      
        1 cggggctgca ggacggcgat cgcatcgtca gcgtcgatga ccagcgcgtc gcggactggc
       61 aggacttcgc cacgcgcgtg cgcgcgaagc cggaagccga cctgcgcctg cagatcgagc
      121 gcgacggcag cccgctcagc gtgcgcctgc gcccggtccg catcgagacc gcgcaggggg
      181 ccgtcggcca gatcggcgcc tttgcggcag tgccgcagtc gctcgccgac tccctgcgcg
      241 tcgtggtacg gtatggcccg atcgatgcag cggttgcagc cgtcgagcgc acttgggaaa
      301 tgtcggcgtt cacgctgcgc atgctcgggc ggatgctgac ggggcgtgcc tcgctggaca
      361 acctcagcgg gccgctgacc atcgcgcaga tggccgggca gtcggcatcg atcggtctgt
      421 ccgccttcct gtcgttcctg gccctggtca gcatcagcct cggtgtgctg aatctgctgc
      481 cggtgccggt gctcgatggc gggcatctgg cgtggtatgc catcgaaacg atcaagggca
      541 gcccggtttc cgaggctgtc cagagcgccg ggcagcgggt cgggctggcc gtgctgctgg
      601 tgatgatggg cctggctttc tacaacgatc tggcgcggtt gttcgggccc tgagcaaagg
      661 gcgccggacg gctgcggtcg ggtctgccgt ccgtgcgatc catgactcca atcctgcgta
      721 acttcctgct gtccgccagt ctcgccgcgc ctgcctgcat cgtgcaggct gcggaattca
      781 tcgtgcgcga catccgtgtc gagggcctgc agcgcatctc ggcgggcacg gtgttcagct
      841 atctgccggt gcagaccggc cagcgcgtgc gtcaggagga tttcccggat ctgatccgca
      901 cgctgttccg caccggcttc ttcacggacg tctcgctgca gcgcgacggc gacgtgctgg
      961 tggtcaacgt cgtcgagcgc ccggcgatcg ccgaggtcaa ggtcaccggc aacaaggaca
     1021 tctcgaccga ggacctggaa aagtccctga agacggtcgg tctcgccgaa ggccgcacct
     1081 tcgaccgggc gctgctcgac aaggtcgagc aggaactcaa ccgccagtac tacgcgcgcg
     1141 gcaagtacgc ggtgaaggtc gacagccagg tgcggccgct cgagcgcaac cgcgtggcga
     1201 tcacgatcga catctccgaa ggcatcgcgg cgcgcatcaa gcagatcaac atcgtcggcg
     1261 ccaaggcctt tgatgaggac gacctgctcg acctgttcca gagttcgacc agcaactgga
     1321 cctcgctgct gtcgaaggac gaccagtact cgaagcagaa actcggcggc gatctcgaaa
     1381 ccctgcgctc gtattacctc gaccgcggct acctgcgctt caacatcgat tcgacgcagg
     1441 tcacgatcac gccggacaag aaggacatct acatcacgat caacatcgcc gagggcgatc
     1501 gctacacggt caaggacgtg cgcctcgccg gtgaaagccc ggtgccgctc gatcagctga
     1561 aaccgctggt caagctgcag cccggccaga ccttctcgcg cgcgacgctc aacgagagca
     1621 ccaaggcgat caccgaccgg ctcggcgatg acggctatgc cttcgccaac gtcagtgccg
     1681 tgccgcagat cgacgatgcc ggcaagaccg tgtcgctggc cctgaacgtc gatgccggcc
     1741 ggcgcgtgta cgtgcgccgc atcaacttca agggcaacat caagacccag gacgaggtgc
     1801 tgcgccgcga gatgcgccag accgagggct cgtggtttgc gacccgcgat gtcaatcgat
     1861 ccaaggagcg tctggagcgt ctggcctttg tgcaggaggt cacggtcgaa actccggccg
     1921 tacccggcac gccggatcag gtcgatctga attacagcgt caccgagcgc ccggccggca
     1981 gcctgagctt cggcatcggc tacggccagg atgccggcgt gctgctcagt gccagtgtct
     2041 cgcagcagaa cttcctcggc accggcaacg cgctgagctt cacgttcaac aacagccaga
     2101 tcaacacgat ctacagcttc agctacacga atccgtactt cacgcccgat ggcgtcagtg
     2161 ccggcatccg ggccagctac cgtgagaccg acgcttccga agccaacctc gcgagctacc
     2221 tgaccaacga attcgagacg atggcgacct tcggcttccc gctgaacgag accgacacgg
     2281 tcaatctcgc gttcggcatc tccggcatca agctcaagac caacggcgac aacacgccgc
     2341 agggcatcta cgactacatc gacgagcatg gcgagcgcca cgtcggtccg cgcttcgaac
     2401 tgagctgggc acgcgacacg cgcaacaagg ccgtcttcgc cgaccgcggc tcgtacaacc
     2461 gcctgtcgat cgaatcgatg ctcggtggcg acgacagctg gtacaagctc ggttaccggc
     2521 acagcaccta tctgccgctg accgattcgc tgacgctgct cggccatttc gacttcggct
     2581 acggtgaggg tttcggcaat accgacacgc tgccgttcta cgagaacttc tacgccggcg
     2641 gcctgcgcac cgtgcgcggc tacaagacca acacgctcgg cccgcgctgg gacgatccga
     2701 atccggacct gcgtgacccg aagggtggtg cgctgcgcac ggccggcggg gtcgaggtgc
     2761 tgttcccggc gccgttcttc aaggacaaca agaacctgcg cttctcgacc ttcttcgaca
     2821 tcggcaacgt gtatgcggaa gtgaacgact tcgatgccgg cgaactgcgc tattccgccg
     2881 gcttgtcgat gcagtggatc acgccgctcg gaccgctggt gatgtcggtc gcccagccgc
     2941 tcaacaagaa ggacggcgac aagaccgagc cgttccagtt caccttcggc gtgccgttct
     3001 gacggcccgc gtcgacccga ggggaagatc gtcttgaagg ccttgctgcg tgtccttctg
     3061 ggggctctcg cgctgacgtt cgcgctgccg gcagtggctg cggagctgaa gctcggtttc
     3121 gtcaacgccg cgcgtgtgct gaagagcgca ccgcaggccg aggcggcgct gaaccggctc
     3181 gaaaaggagt tctcgccgcg cgacaaggcg ctggtcgagg cgcagaaggc gctgcgcaag
     3241 ctggaggaca ggctctccaa ggacggcgcc acgctcagcg acgccgaacg gcgcgacctc
     3301 gaacgcgaca tcatctcgca gcggcgggac gtgaagcgcg cgcaggacga gttccgcgag
     3361 gacttcaaca tccggcgcaa cgaggaaatg gccaagctgc agaagctgat ccaggacgcg
     3421 atcaccgcgc tcgcccagga ggaacgctac gacctggtgc tcaacgacgg cgcagtgatc
     3481 ttcgcgagcg aggccgtcga catcaccgac aaggtgctga agcgcctgtc ggccgtgcac
     3541 ggcaactgag ccggtccggg gtcgacgggg tgcatacgct cgcggaactg gcgcaggtcg
     3601 ccggcgcgca actgcgcggt gacgcggcga cgccgatcga tggcgtcggc acgctcgccg
     3661 cggcccggcc cgggcagatc ggcttcctcg ccaatccgaa gtaccggcag ctcctgtccg
     3721 gcacgcaggc gagcgcggtg atcctggggg ctgaggatgc cggggcctgt ccggtcgcct
     3781 gcctgatcgc cgacaacccg catctcgcct atgcccgggt cgcggcgctg ttcgatccgg
     3841 ccgccggcag gcagcagggc gtgcatgcgc gggcctgcgt cgatgcggcc gccgaagtcg
     3901 ccgacgatgc ctggatcggc ccgatggccg tcatcgaggc cggggccgtg atcgggccgc
     3961 gcgtgcaggt cgggccgggc tgcgtggtcg gggcgcgcac gagcatcggt gccgacagcc
     4021 ggctggtcgc caacgtcacg gtgctgcacg actgcgtgct cggcgagcgc gtgctcgtgc
     4081 atccgggcgc ggtgatcggc gccgatggtt tcgggctcgc gaatgaccgt ggctgctggg
     4141 tcaaggtgcc gcagctcggg cgcgtcgtga tcggcaacga cgtcgagatc ggcgtcaaca
     4201 ccgccgtcga ccgtggcgcg ctcgaggaca ccgtgctcgc cgacggcgtc aagctcgaca
     4261 acctga
//
LOCUS       DAOQJH010000149         4255 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_12574_length_4255_cov_4.712857, whole genome shotgun sequence.
ACCESSION   DAOQJH010000149 DAOQJH010000000
VERSION     DAOQJH010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 4255)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4255)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4255
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12574_length_4255_cov_4.712857"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..138)
                     /locus_tag="PLQ18_04650"
     CDS             complement(<1..138)
                     /locus_tag="PLQ18_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019079406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HNE00976.1"
                     /translation="MKMTGAELIIRLLERQGITTVAGIPGGAILPLYDALGASTQIRH
                     VL"
     gene            complement(502..912)
                     /locus_tag="PLQ18_04655"
     CDS             complement(502..912)
                     /locus_tag="PLQ18_04655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK family protein"
                     /protein_id="HNE00977.1"
                     /translation="MKPAQLARFERKARLLARSEAGRSALAEAGDDWLAHTATVDRTV
                     TFVAEHRTPILLGAGALLLYLARGKPKPRRRPGREDRAGRDEPSAAGWLARLMIVLRL
                     ARTLQTVARHLPRPHPRAVASVSRPRPDAPRGLG"
     gene            complement(909..1295)
                     /locus_tag="PLQ18_04660"
     CDS             complement(909..1295)
                     /locus_tag="PLQ18_04660"
                     /inference="COORDINATES: protein motif:HMM:NF018980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HNE00978.1"
                     /translation="MRAGPAASVYSAGRGVLTALLGLIETRLALFALELEEERERLVQ
                     IGIVAGIALMCAALAIGCTALFVIVLFWDTHRLLAIAGVGGAGLLGLGLCLLWIARRG
                     RHPLLIATRTELAADLDRLRGRGPQP"
     gene            complement(1299..1622)
                     /locus_tag="PLQ18_04665"
     CDS             complement(1299..1622)
                     /locus_tag="PLQ18_04665"
                     /inference="COORDINATES: protein motif:HMM:NF017742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 family protein"
                     /protein_id="HNE00979.1"
                     /translation="MSIKDSQTAQSADRVADEWNNLIAALEGLAAAAAADGSERYAEV
                     RQRAEAALASARARAEANARGAQDRVHDLASQADVYVRQNPWQAIGIGAGIGLIAGLL
                     LSRKG"
     gene            complement(1961..2125)
                     /locus_tag="PLQ18_04670"
     CDS             complement(1961..2125)
                     /locus_tag="PLQ18_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012026284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HNE00980.1"
                     /translation="MLHYTIVFLVVAIIAGVLGFTGIAAGAAEIAKILFFLFLILFVL
                     SLIFGRSRVP"
     gene            2503..>4255
                     /locus_tag="PLQ18_04675"
     CDS             2503..>4255
                     /locus_tag="PLQ18_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HNE00981.1"
                     /translation="MTDRSDRLRELLEQRILILDGAMGTMIQRHKLSEADYRGARFAD
                     WPRDLKGNNDLLVLTKPEVIRGIHAAYLEAGADLIETNTFNATRVSMADYGMEDLVPE
                     LNREAARLARAVCDEYASPDKPRFVAGILGPTSRTASISPDVNDPAARNVTFDELRTC
                     YVEAARALVEGGADILMVETIFDTLNAKAALFAIEELFDELGHRLPVMISGTITDASG
                     RTLSGQTTEAFYHSLRHVQPLSFGLNCALGPDLLRPYVAEMARVAECFVSVHANAGLP
                     NEFGEYDLEPAAMAVQVREWAESGFVNVVGGCCGTSPDHIRAIAGAVAGLPPRRRPDI
                     APACRLSGLEPFTITADSLFVNVGERTNVTGSAVFKRLIKEGNYTAALDVARQQVEAG
                     AQIIDVNMDEGMLDSEAAMVTFLNLIASEPDISRVPVMIDSSKWEIIEAGLKCVQGKA
                     VVNSISLKEGEAKFIEQAKRVRRYGAAVIVMAFDETGQADTLARKREICARSYQVLTE
                     TVGFPAEDIIFDPNIFAVATGIDEHANYAVDFIEATGWIKAHLPHALISGGVSNVSFS
                     FRGNNPVREAIHSVFLYH"
BASE COUNT          694 a         1454 c         1488 g          619 t
ORIGIN      
        1 cagcacgtgg cggatctgcg tcgaggcgcc gagcgcgtcg tacagcggca ggatcgcgcc
       61 gcccgggatg ccggcgacgg tcgtgatgcc ctgtcgttcc agcaggcgga tgatgagttc
      121 ggcgcccgtc atcttcatgg tctgcgtact ccgtcgtcac aagtggcgtt gcgggggctc
      181 ggggccggcc gggacgggga ggacgcagaa acgagaaccc ccgccggcct gtcgcggccg
      241 gcgggggttc tggggttcgg aggaaactgc ctcgacgctc agacccgggc ggacgcgacc
      301 gcgtgcgcta ccagtaccag gcttacgcgt acgacgacgg ccgcgagaag gctttcgcgg
      361 tcgtgacggg cgtgggcagg caggcgggcg gcggtcatgg ctcgggtgtt tcgcaaggcg
      421 gtggccgttt cgggacgcgc actgtagccg cgacggcagg cgcgaggcaa gccggaaatc
      481 ggcgcggccg gccttggggt ttcagccgag gccgcgcggc gcatcgggcc gcggtcgcga
      541 cacgctggcg acggcgcgcg gatgcgggcg cggcaggtgg cgggcgaccg tctgcagcgt
      601 gcgcgcgagg cgcagcacga tcatcagccg cgccagccag ccggcggccg agggttcgtc
      661 acgaccggcc cgatcctcgc ggcccgggcg ccggcgcggt ttcggcttgc cgcgcgccag
      721 atacaacagc aaggcgccgg cgccgagcag gatcggcgtg cgatgctcgg cgacgaaggt
      781 cacggtccgg tcgacggtcg ccgtgtgcgc cagccagtcg tcgccggctt ctgccagcgc
      841 cgaacggccg gcctcggagc gcgccagcag ccgggccttg cgctcgaaac gggcgagctg
      901 cgccggcttc atggctgcgg cccgcgcccg cgcaggcggt cgaggtcggc cgcgagctcg
      961 gtgcgcgtcg cgatcagcag cgggtgacgg ccgcgcctgg cgatccagag caggcacagg
     1021 cccagaccga gcagcccggc gcccccgacg ccggcgatcg caagcagccg gtgcgtgtcc
     1081 cagaacagca cgatcacgaa cagcgccgtg cagccgatcg ccagcgccgc gcacatcagc
     1141 gcgatgccgg caacgatgcc gatctgcacg aggcgctcgc gctcctcttc gagctcgagc
     1201 gcgaacagcg ccagtcgggt ctcgatcagc ccgagcagcg cggtcagcac gccgcgaccg
     1261 gcgctgtaca ccgaggcggc gggcccggcg cgcatgcgtc agcccttgcg gctcagcagc
     1321 aggccggcga tcaggccgat gccggcgccg atgccgatcg cctgccaggg gttctggcgc
     1381 acgtagacat cggcctggct ggcgaggtca tggacgcgat cctgcgcgcc gcgggcgttg
     1441 gcctcggccc gcgcgcgcgc cgaggcgagc gcggcctcgg cgcgctgacg gacctcggcg
     1501 tagcgctcgc tgccatcggc agcggcggcc gcggcgagcc cttcgagcgc ggcgatcagg
     1561 ttgttccatt catcggcgac gcggtcggcg gactgggcgg tttgtgaatc cttgatggac
     1621 atgggcgtga cctcaacggg caagggggcc gggctgtccg gcgccgtggt ggcgcggcat
     1681 cggccgtggt taatgcgaca gcgtagaaag ggaatcgggc agtgcaagcc ggcgcttcga
     1741 catttgcgtg catcctttca ggaaacacgt ccgaagcacg ctccggtttc gtggttccgc
     1801 agccaccgga tgcttattca attacacagc cggtcacgga aattccgtaa ccggctgcaa
     1861 ggcattacga ggtcagtgtc cggtcgaaac cggtctgcgg atcggacccg acggcggccg
     1921 gatcgttccg cggatcgcca ctgacggcct ggagcgcggc tcagggcacc cgcgaacggc
     1981 cgaagatcag cgacagcacg aacaggatca ggaacaggaa gaacagtatc ttggcgattt
     2041 cggcagcgcc ggcggcaatg ccggtgaagc cgagcacgcc ggcgatgatg gcaacgacga
     2101 gaaagacaat cgtgtagtgc agcatgattc gctctccggg cctgtggctc ttatgaggta
     2161 atgacgtgac cggaccgtaa tgctcaggcg ccatggtcgt gtcactggca aatgaccgga
     2221 gcgaatgcgc agcagttcca ttcattgcgt gcattgcgca gacttttttt gaaccgcatg
     2281 aatgatcctg tctgcgccga ggcagaaaat catcacctgt aagatcatcg gcgtgcattc
     2341 agccggcggc ggccgggccg tgcgatgccg ccgcacccgc ccgcgccgca gccgtccgga
     2401 cgcacggcaa gcctgcgatc caccggctga tttgccctgc ccgggcggca cggacacaat
     2461 gcgcggtcct gtttcccgag catcccccgc tggagccctc cgatgaccga ccgtagcgac
     2521 cgcctgcgcg aactgctgga gcagcgcatc ctgatcctcg acggcgcgat gggcacgatg
     2581 atccagcgtc acaagctcag cgaggccgac taccgcggcg cgcgcttcgc cgactggccg
     2641 cgcgacctca agggcaacaa cgatctgctg gtgctgacga agccggaggt gatccgcggc
     2701 atccacgccg cctacctcga agccggcgcc gacctgatcg agaccaacac cttcaacgcc
     2761 acgcgcgtgt cgatggccga ctacggcatg gaagacctgg tgccggagct caaccgcgag
     2821 gccgcgcgtc tggcgcgggc ggtgtgcgac gagtacgcga gccccgacaa gccgcgcttc
     2881 gtcgccggca tcctcggccc cacgtcacgc acggccagca tctcgcccga tgtcaacgat
     2941 ccggccgcgc gcaacgtcac cttcgacgaa ctgcgcacct gctacgtcga ggccgcgcgc
     3001 gcgctggtcg agggcggggc cgacatcctg atggtcgaga ccatcttcga cacgctgaac
     3061 gccaaggccg cgctgttcgc gatcgaggaa ctgttcgacg aactcgggca ccgcctgccg
     3121 gtgatgatct ccggcacgat caccgatgcc tccgggcgca cgctgtcggg ccagaccacc
     3181 gaagccttct accactcgct gcgccacgtg cagccgctca gcttcggcct gaactgcgcg
     3241 ctcggccccg acctgctgcg cccctacgtc gccgagatgg cgcgcgtcgc cgagtgcttt
     3301 gtctcggtgc acgccaacgc cgggctgccg aacgagttcg gcgagtacga cctcgaaccg
     3361 gcggcgatgg ccgtgcaggt gcgcgagtgg gcggagtcgg gcttcgtcaa cgtcgtcggc
     3421 ggctgctgcg gcacctcgcc ggatcacatc cgcgcgattg ccggcgccgt cgccggcctg
     3481 ccgccgcgcc ggcggcccga catcgcgccg gcctgccggc tgtcggggct ggagccgttc
     3541 acgatcacgg cggattcgct gttcgtgaac gtcggcgagc gcaccaacgt caccggctcg
     3601 gcggtgttca agcgcctgat caaggaaggc aactacaccg cggcgctcga tgtcgcgcgc
     3661 cagcaggtcg aggccggtgc gcagatcatc gacgtcaaca tggacgaggg catgctcgat
     3721 tccgaagcgg cgatggtgac cttcctgaac ctgatcgcct cggagccgga catctcgcgc
     3781 gtgccggtca tgatcgattc ctcgaagtgg gaaatcatcg aggccggcct caagtgcgtg
     3841 cagggcaagg ccgtcgtcaa ctcgatctcg ctgaaggaag gcgaggcgaa gttcatcgag
     3901 caggcgaagc gcgtgcgccg ctacggcgcc gccgtcatcg tcatggcctt cgacgagacc
     3961 ggccaggccg acacgctggc gcgcaagcgc gagatctgcg cacgttcgta ccaggtgctg
     4021 accgaaaccg tcggcttccc ggccgaggac atcatcttcg acccgaacat cttcgcggtc
     4081 gccaccggca tcgacgagca cgccaactac gccgtcgact tcatcgaggc gaccggctgg
     4141 atcaaggcgc acctgccgca cgcgctgatc tccggcggcg tcagcaacgt cagcttcagc
     4201 ttccgcggca acaacccggt gcgcgaggcg atccactcgg tgttcctgta ccacg
//
LOCUS       DAOQJH010000150         4230 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_12683_length_4230_cov_4.592335, whole genome shotgun sequence.
ACCESSION   DAOQJH010000150 DAOQJH010000000
VERSION     DAOQJH010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 4230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4230
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12683_length_4230_cov_4.592335"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="PLQ18_04680"
     CDS             complement(<1..184)
                     /locus_tag="PLQ18_04680"
                     /inference="COORDINATES: protein motif:HMM:NF015578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbtH family NRPS accessory protein"
                     /protein_id="HNE00982.1"
                     /translation="MGVVVDTAIRYAVMIGSNGEYSLCRAERCPPPGWRLEGTEGTRS
                     ECMACVQAHAAPAVFAA"
     gene            complement(342..1232)
                     /gene="draG"
                     /locus_tag="PLQ18_04685"
     CDS             complement(342..1232)
                     /gene="draG"
                     /locus_tag="PLQ18_04685"
                     /EC_number="3.2.2.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014239791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047407 - ADP-ribosyl-[dinitrogen
                     reductase] hydrolase activity [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosyl-[dinitrogen reductase] hydrolase"
                     /protein_id="HNE00983.1"
                     /translation="MYDSDVRDRALGAYLGLALGDALGATVEFLTAREIAAQHGRHDE
                     IKGGGWLKLRAGAVTDDTQMSMALGLALIESGGWQLTAIAEAWAEWLRSKPADVGNTC
                     RRGIRRYLKDGSLLTPESADAGNGAAMRNVPVTIVTLGHPRALVKRSLEQARFTHHNP
                     LSDAATVTLGQMTQTLMLGGTTADCRLQADALIATHRQFRFEPYPGQAGGYIVDTVQT
                     VFDAFFAEEDFEACLVRTVNRGGDADTTGAIVGQLAGARCGASRLPRRWLKKLDPSVH
                     AAIVAQTDGLLALAAAAAAC"
     gene            complement(1256..2116)
                     /locus_tag="PLQ18_04690"
     CDS             complement(1256..2116)
                     /locus_tag="PLQ18_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020494032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)--dinitrogen-reductase
                     ADP-D-ribosyltransferase"
                     /protein_id="HNE00984.1"
                     /translation="MSLTRTGTENTPHTPEPTPLSRIGHSTPLVGVPAAVIASLAYND
                     APVPLVIPAVRETNRALYSKLAECPDARSAGQIFQDYMDVVFGLARPDEPACGACRRF
                     RASYLRLLKGWAFDASNPEGAVLKGWVESRFGLFPTWHKAALTRFSSPQWIAYVEEKM
                     APRFHNNAVHAQLDLLYSFCQWRLEHFPLLPPPARHLRLYRGVNDLTEHPLVQRIDKR
                     TAQLRLNNLSSFSADRDRAGEFGDCILEAEVPLTKILFFNDLLPKHMLKGEGEYLVIG
                     GDYRVRTSWL"
     assembly_gap    2255..2354
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2355..2562)
                     /locus_tag="PLQ18_04695"
     CDS             complement(<2355..2562)
                     /locus_tag="PLQ18_04695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE00985.1"
                     /translation="MTGNELIWINPGRCPELADRTRVRARVLRHVDCSRFPGSAICRL
                     WAAAGGSRHDKADMNADMNTDTTAD"
     gene            2818..3693
                     /gene="nifH"
                     /locus_tag="PLQ18_04700"
     CDS             2818..3693
                     /gene="nifH"
                     /locus_tag="PLQ18_04700"
                     /EC_number="1.18.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016611 - iron-iron nitrogenase complex
                     [Evidence IEA];
                     GO_component: GO:0016612 - molybdenum-iron nitrogenase
                     complex [Evidence IEA];
                     GO_component: GO:0016613 - vanadium-iron nitrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0016163 - nitrogenase activity [Evidence
                     IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase iron protein"
                     /protein_id="HNE00986.1"
                     /translation="MSTLRQIAFYGKGGIGKSTTSQNTLAALAEMGQKILIVGCDPKA
                     DSTRLILHAKAQDTVLSLAAEAGSVEDLEIEDVMKIGFRDIRCVESGGPEPGVGCAGR
                     GVITSINFLEENGAYDGVDYVSYDVLGDVVCGGFAMPIRENKAQEIYIVMSGEMMAMY
                     AANNISKGILKYASSGGVRLGGLICNERKTDKELELAEALAGKLGTKLIHFVPRDNVV
                     QHAELRRMTVIEYDPNCKQAGEYRQLAEKVHNNAGKGVIPTPITMDELEDMLMEFGIM
                     TKEDESIVGKAASAA"
     gene            3838..>4230
                     /locus_tag="PLQ18_04705"
     CDS             3838..>4230
                     /locus_tag="PLQ18_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010959595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase component 1"
                     /protein_id="HNE00987.1"
                     /translation="MSSTAEDIKARNKALIAEVLEVYPEKAKKKRQKHLNAYEGGKPD
                     CGVKSNIKSLPGVMTIRGCAYAGSKGVVWGPIKDMVHISHGPVGCGQYSWASRRNYFI
                     GTTGIDSFGTMQFTSDFQEKDIVFGGDKK"
BASE COUNT          702 a         1310 c         1405 g          713 t
ORIGIN      
        1 cggctgcgaa aaccgcgggc gcggcgtgcg cctgcacgca ggccatgcat tccgagcgcg
       61 tgccttcggt gccttcgagc cgccagcccg gcggcggaca gcgttcggcg cgacagagtg
      121 aatattcgcc gttgctgccg atcatcacgg catagcggat cgcggtatcg acgacgacgc
      181 ccatggctgt cctcctgcag gccgggtccg gatgcgcggg gttgcgaccg gtacctgctg
      241 gccttttgcc agcaaatgat gcactgcggc aagcccggat tttgtgcggg aatccgggca
      301 ggagttcccc ggaaattcca cgcaggccgt gcttgccggc ctcagcaggc cgcggcggcg
      361 gcggccagtg ccagcagccc gtcggtctgc gcgacgatcg ctgcgtgcac ggacggatcg
      421 agcttcttca gccagcgccg cggcaggcgg ctggcgccgc agcgggcgcc ggcgagctgg
      481 ccgacgatcg cgccggtggt gtcggcgtcg ccgccgcggt tgacggtgcg gaccagacag
      541 gcctcgaagt cctcctcggc gaagaaggca tcgaacaccg tctgcacggt atcgacgatg
      601 tagccgccgg cctggcctgg atagggttcg aagcggaact gccggtgcgt cgcgatcagc
      661 gcgtcggcct ggaggcggca gtcggcggtc gtgccgccga gcatcagcgt ctgtgtcatc
      721 tgcccgagcg tgacggtggc ggcgtcggag agcgggttgt gatgcgtgaa gcgcgcctgt
      781 tccagcgaac gcttgaccag cgccctcggg tggccgagcg tgacgatggt caccggcacg
      841 ttgcgcatcg ccgcaccgtt gccggcgtcg gcgctctccg gagtcagcag cgagccgtcc
      901 ttcaggtagc gccggatgcc gcgccggcag gtgttgccga catcggccgg cttgctgcgc
      961 agccactcgg cccaggcttc ggcgatcgcc gtcagctgcc agccgccgga ctcgatcagc
     1021 gccagcccga gcgccatcga catctgcgtg tcgtcggtga cggcgccggc cctgagcttc
     1081 agccagccgc cgcccttgat ctcgtcgtgc cggccgtgct gcgcggcgat ttcgcgcgcg
     1141 gtcaggaatt ccacggttgc accgagcgca tcgccgagcg ccaggccgag gtaggcgccc
     1201 agggcgcggt cgcgaacgtc actgtcgtac atcgcagggc tccgggcatg cggcatcaga
     1261 gccagctggt gcgcacgcgg tagtcgccgc cgatgacgag gtactcgccc tcgcccttga
     1321 gcatgtgctt cggcagcagg tcgttgaaga acaggatctt ggtcagcggc acctcggctt
     1381 cgaggatgca gtcgccgaac tcgccggcgc ggtcgcgatc ggcgctgaag gaactgaggt
     1441 tgttcaggcg cagctgcgcc gtgcgcttgt cgatgcgctg caccagcggg tgctcggtga
     1501 gatcgttgac gccgcggtac agccgcaggt gccgggccgg gggcggcagc agcgggaaat
     1561 gttcgagccg ccactggcag aagctgtaca gcaggtcgag ctgcgcgtgc accgcgttgt
     1621 tgtggaagcg cggcgccatc ttctcctcga cataggcgat ccactgcggg ctggagaagc
     1681 gcgtcagcgc cgccttgtgc caggtcggga acaggccgaa gcggctctcg acccagccct
     1741 tgagcaccgc accctcgggg ttgctggcgt cgaaggccca gcccttgagc aggcgcaggt
     1801 agctcgcgcg aaagcgccgg caggcgccgc aggccggttc gtccgggcgg gcgaggccga
     1861 acaccacgtc catgtagtcc tggaagatct gcccggccga gcgcgcgtcc gggcattcgg
     1921 cgagcttcga gtacagcgcc cggttggtct cgcgcacggc cgggatcacc agcggcaccg
     1981 gggcgtcgtt gtacgcaagg ctcgcgatca ccgccgccgg cacgccgacc agcggcgtgc
     2041 tgtggccgat gcgcgacaac ggtgtcggtt cgggggtgtg cggcgtgttt tcggtgccgg
     2101 tccgggtcag gctcatcagg caactcctcc tcgttcctcg ttcctgctcg ttcttgtcgt
     2161 ggtcccgacg cctgcgctgc ggccggcatc gccggtcctg tcacggaccc ggccgctgcc
     2221 gggctgcgcc ctgccggttt tcgtgtcggc tgtcnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnntgtcgg ctgtcgtgtc ggtgttcatg tcggcattca tgtcggcttt
     2401 gtcgtgccgg ctgccgccgg ctgcagccca aagccgacaa atcgccgatc cgggaaaccg
     2461 tgagcaatcc acatgccgga gcacgcgcgc gcgtacgcgc gtgcggtcgg ctaattcagg
     2521 gcatcggccg ggattgatcc agatcaattc attgccggtc attcaggaaa cgggacgggt
     2581 agccggcggc ctgcacgcgg tctgtcggca ttcggccgaa tgtccggatt tgtcggagag
     2641 gacacacggt gcgacagtca tgcagccttg tgtccggagt ctttttatcc attttcaatc
     2701 aatgacttgt attattttat cgagtttggc acgccggttg ctcattagct cgtggggagc
     2761 agcaaggtcc cgatggttaa caaaatcctg aaacctgatc tcgaaggaag acggcaaatg
     2821 agcacgctta ggcagattgc cttctacggc aagggtggaa tcggcaagtc caccacctcc
     2881 cagaacaccc tcgccgcgct cgcggaaatg ggtcagaaga tcctgatcgt cggctgcgac
     2941 ccgaaggctg actccacccg cctgatcctg cacgcaaagg cccaggacac ggtgctctcg
     3001 ctcgccgccg aagccggcag cgtcgaggat ctggaaatcg aagacgtcat gaagatcggt
     3061 ttccgcgaca tccgctgcgt cgagtcgggt ggtccggagc cgggcgtcgg ctgcgccggt
     3121 cgcggtgtca tcacctcgat caacttcctg gaagagaacg gcgcgtatga cggcgttgat
     3181 tacgtctcct acgacgtgct cggcgacgtg gtctgcggcg gtttcgcgat gcccatccgc
     3241 gagaacaagg cccaggaaat ctacatcgtc atgtccggcg aaatgatggc catgtacgcg
     3301 gccaacaaca tttccaaggg catcctgaag tacgcgtcct cgggcggcgt gcgcctcggc
     3361 ggcctgatct gcaacgagcg caagaccgac aaggaactgg aactggccga agcgctggcc
     3421 ggcaagctcg gcaccaagct cattcacttc gtgccgcgtg acaacgtcgt gcagcacgcc
     3481 gagctgcgtc gcatgaccgt catcgaatac gacccgaact gcaagcaggc cggcgagtat
     3541 cgtcagctgg cggaaaaggt tcacaacaac gccggcaagg gcgtcattcc gaccccgatc
     3601 acgatggacg aactcgaaga catgctcatg gagttcggca tcatgacgaa ggaagacgag
     3661 tccatcgtcg gcaaggcggc gtcggcggcc tgatcggcgc cgcgagccct ccctccgaaa
     3721 atgcggggga gggccgggac gggggtaaac cacacgcggc ggcatcccgt tccgtcccgc
     3781 ccatcggcga atgggtttgc cgcatcgtaa acaggattcc gattctggga gggccagatg
     3841 agctcgacgg ctgaagacat caaggcgcgc aacaaggcgc tgatcgcgga agttctcgaa
     3901 gtttatcccg agaaggcgaa gaagaagcgc cagaagcatc tgaacgcgta tgaaggcggc
     3961 aagccggatt gcggcgtcaa gtccaacatc aagtcgctgc ccggtgtcat gaccatccgt
     4021 ggttgcgcgt acgccggttc caagggcgtg gtgtggggtc cgatcaagga catggtccac
     4081 atctcgcacg gtccggtcgg ctgcggccag tactcctggg cctcgcgccg taactacttc
     4141 atcggcacga ccggcatcga ttccttcggc acgatgcagt tcacgtccga tttccaggaa
     4201 aaggacatcg tcttcggcgg tgacaagaag
//
LOCUS       DAOQJH010000151         4204 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_12812_length_4204_cov_3.855387, whole genome shotgun sequence.
ACCESSION   DAOQJH010000151 DAOQJH010000000
VERSION     DAOQJH010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 4204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4204
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12812_length_4204_cov_3.855387"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1206
                     /locus_tag="PLQ18_04710"
     CDS             <1..1206
                     /locus_tag="PLQ18_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008954312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HNE00988.1"
                     /translation="SLIANTAPVAYGALGTPVITLAKVTGLDVMQLSAMVGRQLPLFS
                     LIVPFWLVWAFCGFRNMIKVWPAVLVAGLFFAIPQFVVSNYFGPELVDVLAAIISLAA
                     LALFLKFVWHPKEVWTSTSGKAEDNVEHVGGHHGYTGGEVLRAWLPWIILSVLVLIWG
                     MPEVKKFWNGVWAPVFEWPGLHNMVQKVPPIVAHPEIEGAKYTFGILSATGTGILIAA
                     VISGFIMGYSPLGMLKVYWETLKTLKYSLITIVAMLALGYVTRYSGTDITLGLAFAQT
                     GVFYPFFGTLLGWLGVALTGSDTAANVLFGGLQKASAQQLGLSPVLMAAANSAGGVMG
                     KMIDAQSIVVASTATRWYGHEGDILRYVFFHSIALACLVGILVTLMAYVPPFTSLVIW
                     PATGGVPVH"
     assembly_gap    1654..1753
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1754..1945
                     /locus_tag="PLQ18_04715"
     CDS             <1754..1945
                     /locus_tag="PLQ18_04715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE00989.1"
                     /translation="SPASNKTTKGGGEHPQMLHCIIWIIRRTVNGFDKQNLFPSSEHK
                     VMRTGAVQPSAVPAARQAW"
     gene            complement(1906..2373)
                     /locus_tag="PLQ18_04720"
     CDS             complement(1906..2373)
                     /locus_tag="PLQ18_04720"
                     /inference="COORDINATES: protein motif:HMM:NF021049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiD C-terminal domain-containing protein"
                     /protein_id="HNE00990.1"
                     /translation="MESRHPLDAFFAQHIPLTQAMGLHVAGRDGSGLRLAAPLAPNLN
                     DKGTAFAGSLAAIVTLAGWALTTLVLREAGIEDAQVAVGRAETDYRRPVRSALLADCR
                     QPPPAETERFLGDYRSRGRARWELEIVLMQDDAVAVFQRARYHAWRAAGTADG"
     gene            2535..3122
                     /locus_tag="PLQ18_04725"
     CDS             2535..3122
                     /locus_tag="PLQ18_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNE00991.1"
                     /translation="MTTVAIDAALARRWSPRALDPARPLARAHLQALLEAARWAPSCF
                     GDQPWRFICWDRSRDPAGFAAALATLSEGNRVWAQHAAVLLLAVACADFRHNGQPNRW
                     AQYDTGAASENLCIQAAALGLAAHQMGGFDAAAVAKLAGLPERATPMAMIAIGHPAPA
                     DVLPEALRTRETAARTRLALSGIAFEGGWAQPLGD"
     gene            3133..4203
                     /locus_tag="PLQ18_04730"
     CDS             3133..4203
                     /locus_tag="PLQ18_04730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE00992.1"
                     /translation="MSAPAGAGVDRFLVRAGLFLLLLAGLALLLRAPLANAFRISPYL
                     NGLILVLFLFGAGYTLRCLGLLRREQAACARVARLAAEVRAGTRAAGEAAELMTLPSL
                     GAVGEFLARVRLTLRQADAQQTLPFLLETLAGRGEDARALVRFLGGTLVLIGLIGTFY
                     GLLLSTEGVRDLLGTAAGGDGADWLANLRARLSTPLTGMGTGFATSLFGLVCSAALGF
                     LELQLFHPQSAVTTRLETLVVTELLPVWQTPALIGREQPVAPRYLLAVAEALIERLDR
                     VAGLLEAAAARDDAGRLAQAVARGDERVQALGVRLEREAREQNAALMHELRVIARYMA
                     RDTQPPVVSPQAAEPHDAPPPR"
BASE COUNT          589 a         1413 c         1434 g          668 t
ORIGIN      
        1 tcgctgatcg ccaacaccgc gccggtcgcc tatggcgcgc tcggcacgcc ggtcatcacg
       61 ctcgccaagg tcaccggcct cgatgtcatg cagctctcgg cgatggtcgg tcgccagttg
      121 ccgctgttct cgctgatcgt gccgttctgg ctggtctggg ccttctgcgg cttccgcaac
      181 atgatcaagg tctggccggc ggtgctggtc gcgggcctgt tcttcgcgat cccgcagttc
      241 gtcgtctcca actacttcgg tcccgagctg gtcgacgtgc tcgccgcgat catctcgctg
      301 gccgcgctgg cgctgttcct gaagttcgtg tggcacccga aggaagtctg gacctcgacc
      361 agcggcaagg ccgaggacaa cgtcgagcac gtcggcggtc accacggtta caccggcggc
      421 gaggtgctgc gcgcctggct gccgtggatc atcctgtcgg tgctggtgct gatctggggc
      481 atgcccgagg tcaagaaatt ctggaacggc gtctgggcgc cggtgttcga gtggccgggc
      541 ctgcacaaca tggtccagaa ggtgccgccg atcgttgcgc acccggagat cgaaggcgcc
      601 aagtacacct tcggcatcct gtcggcgacc ggtaccggca tcctcatcgc cgccgtcatc
      661 tcgggcttca tcatgggcta cagcccgctc ggcatgctca aggtctactg ggaaaccctg
      721 aagaccctga agtactcgct gatcacgatc gtcgcgatgc tcgcgctcgg ctacgtgacc
      781 cgctactccg gcaccgacat caccctcggc ctcgcctttg cgcagaccgg cgtgttctac
      841 ccgttcttcg gcacgctgct cggctggctc ggcgtggcgc tgaccggttc ggataccgcc
      901 gccaacgtgc tgttcggcgg cctgcagaag gcttcggcgc agcagctcgg cctgtcgccg
      961 gtgctgatgg ccgcggcgaa cagcgccggc ggcgtcatgg gcaagatgat cgacgcgcag
     1021 tccatcgtcg tcgcctcgac cgcgacccgc tggtacggcc acgaaggcga catcctgcgc
     1081 tacgtgttct tccactcgat cgcgctcgcc tgcctggtcg gcatcctcgt gacgctgatg
     1141 gcctacgtgc cgccgttcac cagcctggtg atctggccgg ccaccggcgg cgtgccggtg
     1201 cactgagcgg cgcagcctga cgacattccc gacactccga acgaaggggc cgcaaggccc
     1261 cttctgcttt tccgggcacc catgtcggtc gctccggcgg agcagggcgg cagtcggcgg
     1321 gcgcgcatcg ggccggaaga catgcactca ttcggatgta tagataaatt tttataccat
     1381 catttaatgc ggtgccgttc aaacaccgat gtcagacgcc agtcatcaga ccgatttcag
     1441 gacgacgagc tgcgctggag aggcaggcga gctccggcac gggagagcgt cccggcgtcg
     1501 gcatccggcc cgcggccggc cgcgcggcag aggcggatca ctgcaggctg gcgtcaggca
     1561 aggacctgaa tgacgcgcaa aaaaagagcc gggcggggag cccggcttca aacaagacca
     1621 ccaaaggagg aggagaacat ccgcagatgt tgcnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnagcccgg cttcaaacaa gaccaccaaa ggaggaggag aacatccgca
     1801 gatgttgcat tgcatcatat ggatcatccg ccggaccgtc aacgggttcg ataaacaaaa
     1861 tttgtttccg tcaagtgaac ataaagtcat gcgaaccggt gccgttcagc catccgccgt
     1921 gccggcggca cgccaggcgt ggtagcgggc gcgctggaac acggcaacgg cgtcgtcctg
     1981 catcagcacg atctccagtt cccagcgcgc ccggccgcgg ctgcggtagt cgccaagaaa
     2041 acgctcggtc tcggccggcg ggggctgacg gcagtcggcg agcagcgcgc tgcgcaccgg
     2101 ccggcggtag tcggtctcgg cacgcccgac cgcgacctgg gcgtcctcaa tgccggcctc
     2161 gcgcagcacc agcgtcgtca gcgcccagcc ggccagcgtc acgatggccg ccagcgagcc
     2221 ggcgaaggcc gtgcccttgt cgttgaggtt cggcgccagc ggggcggcga ggcgcaggcc
     2281 gctgccgtca cgaccggcga cgtgcaggcc catcgcttgc gtgagcggaa tgtgttgggc
     2341 gaaaaaggca tcgagcggat ggcgggattc catgggcggc ggcaggcagc ggagcggggt
     2401 ggatgccaac cttagccttt gcgtccgatg gccgatggcc gatggccgat ggccgctgtc
     2461 cgtcgatccg tccctgcgtg ccggagatcg ccgtgtccga accgatgcct catccgctgc
     2521 ccgggaaacc ggccgtgacc actgttgcca tcgacgcggc cctcgcccgg cgctggagcc
     2581 cgcgcgcgct cgatcccgca cggccgctgg cgcgtgcgca tctgcaggcg ctgctcgaag
     2641 ccgcgcgctg ggcgccgtcc tgcttcgggg atcagccctg gcgcttcatc tgctgggacc
     2701 gcagccgcga tccggccggc ttcgccgcgg cactggcgac gctgtccgaa ggcaaccgcg
     2761 tctgggcgca gcatgcggcc gtgctgctgc ttgccgtcgc ctgcgcggac ttccgtcata
     2821 acggccagcc gaaccgctgg gcgcagtacg acaccggcgc ggccagcgag aacctgtgca
     2881 tccaggccgc tgcgctcggg ctggcggcac accagatggg cggcttcgat gccgcggcgg
     2941 ttgcgaaact tgccggcctg ccggagcgcg cgacgccgat ggcgatgatc gcgatcggtc
     3001 atccggcacc ggccgatgtg ctgcccgaag ccctgcgcac ccgcgaaacc gcggcgcgca
     3061 cgcggctggc gctgtccgga attgccttcg agggcggctg ggcgcagccg ctcggcgact
     3121 gaggccgctg cgatgagcgc tccggccggt gccggcgtcg accgttttct ggtgcgggcc
     3181 ggactgttcc tgctgctgct cgcggggctg gcgctgctgc tgcgcgcgcc gctcgccaac
     3241 gccttccgga tcagccccta cctgaacggc ctgatcctcg tgctgttcct gttcggagcc
     3301 ggttacacgc tgcgctgtct ggggctgctg cgccgcgagc aggcggcctg cgcgcgcgtg
     3361 gcgaggctgg cggccgaagt gcgcgccggc acgcgggctg ccggcgaagc ggctgagctg
     3421 atgacgctgc cgagccttgg tgcggtcggc gaatttctcg cccgcgtgcg cctgacgctg
     3481 cgccaggccg atgcgcagca gacgctgccc ttcctgctcg aaacgctcgc cgggcgcggg
     3541 gaggatgcgc gcgcgctggt gcgcttcctg ggcggcacgc tggtgctgat cggcctgatc
     3601 ggcaccttct acggtctgct gctcagtacc gagggcgtgc gcgacctgct cggcacggcg
     3661 gccggcggcg acggggccga ctggctcgcg aacctgcgcg cgcggctgtc gacgccgctg
     3721 accggcatgg gcaccggctt cgcgacctcg ctgttcggac tggtctgctc ggcggcgctc
     3781 ggcttcctcg aactgcagct cttccacccg cagagcgccg tcacgacccg gctggagacg
     3841 ctggtggtca ccgaactgct gccggtctgg cagacgccgg cgctgatcgg tcgcgagcag
     3901 ccggtggcgc cgcgctatct gctggcggtc gccgaggcgc tgatcgaacg gctcgatcgc
     3961 gtcgccggcc tgctcgaagc tgcggcggca cgcgacgatg ccgggcggct cgcacaggcc
     4021 gtcgcgcgcg gcgacgagcg cgtgcaggcg ctcggggtgc ggctggagcg cgaggcccgc
     4081 gagcagaacg ccgcgctgat gcatgagctg cgcgtgatcg cgcgctacat ggcccgggac
     4141 acgcagccgc ctgtcgtcag cccgcaggcc gcggagccgc acgatgcgcc gccgccgcgc
     4201 tgag
//
LOCUS       DAOQJH010000152         4192 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_12877_length_4192_cov_3.996616, whole genome shotgun sequence.
ACCESSION   DAOQJH010000152 DAOQJH010000000
VERSION     DAOQJH010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 4192)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4192)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4192
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12877_length_4192_cov_3.996616"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(30..830)
                     /locus_tag="PLQ18_04735"
     CDS             complement(30..830)
                     /locus_tag="PLQ18_04735"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017780572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNE00993.1"
                     /translation="MPALLPLVCTDPARHAEAAGLAARYGLPVLADIDALYGTYWLRL
                     DATGLALLGRGADAPGPVQVDWTGGALGHRLRFGGGRGQPLARAIGLKGGACPAVADL
                     TAGLGRDACVLAALGCRVTLVERSPVVCALLDDGIARAAADTGIGSWVRERVQLVHAD
                     GSAWLQAAAQQAQAAPAQRPEVIYLDPMYPHREQSALVKKEMRAFRAVVGDDLDADAL
                     LAPALAVALRRVVVKRPKGAPPLTGRAPSHVIASPNTRYDVYVTLAGA"
     gene            complement(944..2740)
                     /gene="phaC"
                     /locus_tag="PLQ18_04740"
     CDS             complement(944..2740)
                     /gene="phaC"
                     /locus_tag="PLQ18_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012975690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I poly(R)-hydroxyalkanoic acid synthase"
                     /protein_id="HNE00994.1"
                     /translation="MADSNTPPFNLPNVNLPDTQKLAENLGKIAERSQSLVQEFAARD
                     NFQVPDPGIVAKSFLELSQKLLADPAKIIEAQMNFWQEYLNVVQATTRRLAGETVEPV
                     VKPAPDDKRFKDEAWVENAVFDFIKQSYLLAARTIHKTATGVEGLDTKTAQRVEFYTR
                     QYIDALAPTNFALTNPRVVKETLESGGENLIKGLANLLEDLDRGKGKLRVRMTDLEAF
                     EPGKNVAATPGKVVFQTPLMQLIQYTPTTEEVYQKPLLIVPPWINKYYILDLGPKNSF
                     IKWAVDQGFTVFTISWVNPDETLADKNFEDYLKEGTLAALDAIREATGEKEVVAASYC
                     LGGTLLMSTLAYLAAKRDNRIKAATCFTTMLDFSEPGELEVFIDEEQIELIEKHMEEK
                     GYLEGTAMSGVFNMLRANDLIWSFVVNNYLMGKDPMAFDLLYWNSDSTRMPRKMHSFY
                     LRNMYLNNKLKDAGGITLLGQPIDLAKIKTPVFFLSTKEDHIAPWTSTYKGTQLLGGP
                     VKFVLGGSGHIAGVINPATSKKYGFQTNDKLPPNAGDWLAGAEQHDGSWWNHWKTWVE
                     PMVGEKVPARQPGGGKLTPIEDAPGSFVKVRI"
     gene            2955..3572
                     /locus_tag="PLQ18_04745"
     CDS             2955..3572
                     /locus_tag="PLQ18_04745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE00995.1"
                     /translation="MATGGFEGVRRGWLLAVLVAALPLAQADEVEDRVGRGLAAYRKG
                     QYAEALQALAVASSAVQAKLAGEYRNLLPPAPAGWEAEDAEVRAITVPAADGGSGVQL
                     SRRYFREEEELALEVAVDSPLIAGLDLMLSDPKLLASDPAVRPYRIGDNRGLIRRHGD
                     DLEVSVMVGRRVLVRVSGSNLKEDAEAESLLSSIDFAGLSQRLLN"
     gene            complement(3580..>4192)
                     /locus_tag="PLQ18_04750"
     CDS             complement(3580..>4192)
                     /locus_tag="PLQ18_04750"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNE00996.1"
                     /translation="RLLDPELGELRPGAFLDAAERLQLAAALDRAALGIVLDTLATRP
                     GDTHRYVLNLGSASLADDEYLPHLRARLAATGVAAGRLAFDVNARDALAGQPAIGTTM
                     AGLRALGCRVLIDDVGLGLAAPAAWRTLQADGLKLDGALVRALPGGRIEQGFVAAIAA
                     LGQALALPVIAESVETAAQLAALQALGIGHGQGEALGAPQRWA"
BASE COUNT          603 a         1405 c         1506 g          678 t
ORIGIN      
        1 cgccatcggc tgaaatccgc tcgccggctt caggcgccgg cgagcgtcac gtacacgtcg
       61 tagcgcgtgt tcgggcttgc gatcacatgc gagggtgccc gcccggtcag cggcggcgcg
      121 cccttcggcc gcttgacgac gacgcggcgc agcgcgaccg ccagcgccgg ggcgagcagc
      181 gcatcggcat ccaggtcgtc gccgaccacg gcgcggaagg cgcgcatctc cttcttcacc
      241 agcgccgact gttcacggtg cggatacatc gggtcgaggt agatcacctc gggccgctgc
      301 gccggcgcgg cctgcgcttg ctgcgcggct gcctgcagcc aggcgctgcc gtcagcgtga
      361 accagctgca cgcgttcgcg cacccaggaa ccgatgccgg tgtcggcggc ggcgcgggcg
      421 atgccgtcat cgagcagcgc gcacaccacc gggctgcgct cgaccagcgt cacccggcag
      481 ccgagcgcgg cgagcacgca ggcatcgcgc ccgagtccgg cggtcaggtc ggcgaccgcc
      541 ggacaggcgc cgcccttgag tccgatcgcc cgcgccagcg gctggccgcg gccgccgccg
      601 aagcgcagcc ggtgtccgag cgcaccgccg gtccagtcga cttgcaccgg tccgggcgcg
      661 tccgcaccgc gaccgagcag tgcgagcccg gtcgcgtcga gccgcagcca gtacgttccg
      721 tacagcgcgt cgatgtccgc cagcacgggc aggccgtagc gcgcggcaag tccggcagcc
      781 tcggcgtgac gggcggggtc ggtgcagacg agcggcagga gggctggcat ggcggacgtg
      841 gggcgaggct ttgcgtggac gggccgcagg cgcgtgcggc gcggaaaaca aaaaggcgga
      901 ggacgcgaac gtcctccgcc cccgtttcac cggtgcgtgc ggatcagatg cgcaccttca
      961 cgaaggagcc cggggcatcc tcgatcggcg tgagcttgcc gccgcccggc tggcgcgccg
     1021 gcaccttctc gccgaccatc ggctcgaccc aggtcttcca gtgattccac cacgagccgt
     1081 cgtgctgctc ggccccggcc agccagtcgc cggcgttcgg cggcagcttg tcgttggtct
     1141 ggaagccgta cttcttggag gtcgccggat tgatgacgcc ggcgatgtgg ccggagccgc
     1201 cgagcacgaa cttgaccggg ccgccgagca gctgcgtgcc cttgtaggtc gaggtccacg
     1261 gcgcgatgtg gtcttccttg gtggacagga agaacaccgg cgtcttgatc ttggcgagat
     1321 cgatcggctg gccgagcagc gtgatgccgc cggcatcctt gagcttgttg ttcaggtaca
     1381 tgttgcgcag gtagaagctg tgcatcttgc gcggcatgcg ggtcgaatcg gagttccagt
     1441 acagcaggtc gaaggccatg gggtccttgc ccatgaggta gttgttgacg acgaacgacc
     1501 agatcaggtc gttggcgcgc agcatgttga acacgccgga catcgccgtg ccttcgagat
     1561 agcccttctc ctccatgtgc ttctcgatca gctcgatctg ttcctcgtcg atgaacacct
     1621 ccagctcgcc cggctcgctg aagtcgagca tggtcgtgaa gcaggtcgcg gccttgatgc
     1681 ggttgtcacg cttggcggcg agatacgcca gcgtcgacat cagcagcgtg ccgccgaggc
     1741 agtacgaggc ggcgaccacc tccttctcgc cggtggcctc gcggatcgcg tcgagcgcgg
     1801 ccagcgtgcc ttccttcagg tagtcctcga agttcttgtc ggcgagggtc tcgtcggggt
     1861 tgacccacga gatcgtgaac accgtgaagc cctgatccac cgcccacttg atgaaggagt
     1921 tcttcgggcc gaggtcgagg atgtagtact tgttgatcca cggcggcacg atcagcagcg
     1981 gcttctgata gacctcttcc gtggtcggcg tgtactggat cagctgcatc agcggcgtct
     2041 ggaacacgac cttgcccggc gttgccgcga cgttcttgcc cggctcgaag gcttcgaggt
     2101 cggtcatgcg cacgcgcagc ttgcccttgc cgcgatcgag gtcctccagc aggttcgcga
     2161 ggcccttgat gaggttctcg ccgccggatt cgagggtctc cttgaccacg cgcgggttgg
     2221 tcagcgcgaa gttcgtcggc gccagggcat cgatgtactg gcgcgtgtag aactcgacgc
     2281 gctgcgcggt cttggtgtcg aggccctcga cgccggtcgc ggtcttgtgg atcgtgcgcg
     2341 cggcgagcag gtagctctgc ttgatgaagt cgaacaccgc gttctcgacc caggcctcgt
     2401 ccttgaagcg cttgtcatcg ggcgcgggct tgaccaccgg ttcgacggtc tcgccggcga
     2461 ggcgccgcgt cgtcgcctgc acgacgttca ggtattcctg ccagaagttc atctgcgctt
     2521 cgatgatctt cgccggatcg gccagcagct tctgcgacag ctccagaaac gacttggcga
     2581 cgatgcccgg gtccggcacc tggaagttgt cgcgggccgc gaactcctgc acgaggctct
     2641 ggctgcgctc ggcgatcttg ccgaggttct ccgcgagctt ctgcgtgtcg ggcaggttga
     2701 cgttcgggag attgaacgga ggcgtgttgc tgtcggccat gaaggcgctc cctggtggtg
     2761 gattcgccgg tcacggccgg cgctgtcatc gtggaaagcc gggcgggcac gccccggacg
     2821 ggcagggggt gctgcggcgg ggccgtacat taagccttcg caccgcatcg cggaatacgc
     2881 agcgggtgca ggtttgcccg aggccgggca ttgtggaacc atgccgcacg aagatttcgg
     2941 caggtgatcc ggtgatggcg acgggcgggt tcgagggcgt aaggcggggc tggctgctgg
     3001 ccgtgctggt cgcggcgctg ccgctggcac aggcggacga agtcgaggat cgcgtcggcc
     3061 gcgggctggc ggcgtatcgc aagggtcagt acgccgaggc gctgcaggcg ctggccgtcg
     3121 cgagcagcgc cgtgcaggcg aagctcgccg gcgaataccg caacctgctg ccgccggcgc
     3181 cggccggctg ggaagccgag gatgccgagg tccgcgccat caccgtgcct gctgccgacg
     3241 gtggcagcgg cgtgcagctg agccggcgct acttccgcga ggaagaggaa ctcgcgctcg
     3301 aggtcgccgt cgattcgccg ctgatcgccg ggctggacct gatgctgtcc gatccgaagc
     3361 tgctcgccag cgacccggcc gtgcgcccgt accgcatcgg cgacaatcgc ggcctgatcc
     3421 gccgccacgg tgacgatctc gaagtgtcgg tgatggtcgg gcggcgcgtg ctggtgcgcg
     3481 tcagcggcag caacctcaag gaagacgccg aagccgagtc gctgctgtcg tcgatcgact
     3541 tcgccggcct gtcgcagcgg ctgctgaact gacagccctt caggcccagc gctgcggcgc
     3601 gccgagcgcc tcgccctggc cgtggccgat gccgagcgcc tgcagcgcgg cgagctgtgc
     3661 cgccgtctcc accgactcgg cgatgaccgg cagcgccagc gcctgtccca gcgccgcgat
     3721 cgccgcgacg aaaccctgct cgatgcgccc cccgggcagg gcgcgcacca gcgcgccgtc
     3781 gagcttcagg ccatcggcct gcagcgtccg ccacgccgcc ggagccgcga gtccgagccc
     3841 gacgtcgtcg atcagcacgc ggcagccgag cgcccgcagt ccggccatgg tcgtcccgat
     3901 cgccggctgg ccggcaagcg catcgcgggc attcacgtcg aacgcgaggc ggccggcggc
     3961 aaccccggtg gccgccaggc gcgcgcgcag gtgcggcaga tattcatcat cggcgaggct
     4021 cgcgctgccg aggttcagca cgtagcggtg cgtatcgccg gggcgggtcg ccagggtgtc
     4081 gagcacgatg ccgagcgcgg cccgatccag cgccgccgcg agctgcaggc gctcggccgc
     4141 atcgaggaag gcaccgggac gcagctcgcc gagttcggga tcgagcaggc gc
//
LOCUS       DAOQJH010000153         4190 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_12889_length_4190_cov_3.321644, whole genome shotgun sequence.
ACCESSION   DAOQJH010000153 DAOQJH010000000
VERSION     DAOQJH010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 4190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4190
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12889_length_4190_cov_3.321644"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..313
                     /locus_tag="PLQ18_04755"
     CDS             <1..313
                     /locus_tag="PLQ18_04755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE00997.1"
                     /translation="TTLIFAAGHPQPLTRAGEALLAALRERHAPDPGWQRSDPALLLC
                     LALCGAAAVRGLVPGGVGHVLAPPASDDDAPVGLDAGLDPGGSGCDSGGDGGCGSGCS
                     G"
     gene            310..1035
                     /locus_tag="PLQ18_04760"
     CDS             310..1035
                     /locus_tag="PLQ18_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006518558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE00998.1"
                     /translation="MNPDPHRPGTAANDAGPPALSTPAGADCTAALPPACRDPVLAAL
                     YQRLLAYEFDDPQAALPFSRRLARDNGWTHRGALRAIDEYRRFVFLALAAGHPVTPSD
                     QVDQVWHLHLVYTRMYWLDFCPQVLGRSLHHGPTRGGAAEGGRYDEQYRRTLASYERC
                     FGHPPPADLWPAPELRFGRDLGFVRVNRHACWLLPRSPEAWRFVLHRRLGRLLRSLRR
                     TLARFGLASEPAPGPVRDPGRKS"
     gene            1032..2012
                     /locus_tag="PLQ18_04765"
     CDS             1032..2012
                     /locus_tag="PLQ18_04765"
                     /inference="COORDINATES: protein motif:HMM:TIGR04222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="HNE00999.1"
                     /translation="MSASFLRRQHPGRGACLGLLPVLCLVSVSDSAGALDNPLDYDGS
                     HFLGLYVPLMLASLVLAMLLRRWLRLPGPDPRHQPRLDVYETAYLAGGAGRTVDTALA
                     VMALRGEIGASPDGLRLQACGRPQLRDPIERKVFGALTLEGRPPGLHRRVRPVLCAEL
                     DARLRPQGLLPTPVQAAMVRLLPAACTGSVLLLGLLKLAIGLSRHRPVGFLVVCCLVL
                     SIATLVLLLKTVPRSRHGDAVLKQLRSGLSARTVESRQDPRLALVMALFGVAAVAHLL
                     PEDLAEQLAPAGSGGGDSGSGSDGSDSGGGDSGGDSGGGGCGGCGGGGGD"
     gene            2166..3926
                     /locus_tag="PLQ18_04770"
     CDS             2166..3926
                     /locus_tag="PLQ18_04770"
                     /EC_number="2.1.1.-"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HNE01000.1"
                     /translation="MREPRELHFDTRDGSALFYRHWPALDGPGEQALLLLHRGHEHSG
                     RLQHVVDELDLPGFAVFAWDARGHGRSPGPRGHCPGIATATGDLDTFVRHLRDRHGIA
                     PENLVVIAQSVGAVIAATWAHDYAPPVRALVLAAPAFKVKLYVPLARPALRLRYALGG
                     NFEVQSYVKAKFLTHDPARIASYDADPLITRAIAVNMLLGLYEAGERVVEDAAAITLP
                     VLVLVSGADFVVHRAPQRRFFERLGSKVKAYHELPGFYHDTFGELRRSEAIGLVRAFV
                     RKCFDAPFERPDLRTADRHGHTFDEYRRLQAPLPEGSWKIPYYAAVRAGLALGARLAE
                     SIRIGHETGFDSGSTLDAVYRNRPAGFGPPGRLIDRLHLDSAGWAGIRIRKQHVEALL
                     ADAVRALRRAGRPVRIVDIAAGHGRYVLDGLATLPEPLEAADSVLLRDYSPINVAAGR
                     QLIAARGRAATVRFEQGDAFDRDALAALAPAPTVAIVSGRYELYPDNAPLATSLSGLA
                     VALEAGGFLIYTGQPWHPQLELIARALASHRDGAPWVMRRRTQGELDQLVAAAGFDKC
                     TQLIDDAGIFTVSLAVRRAS"
     gene            3923..>4190
                     /locus_tag="PLQ18_04775"
     CDS             3923..>4190
                     /locus_tag="PLQ18_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012437212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HNE01001.1"
                     /translation="MSAAGSAAGVHEARPWPRALLWLAVLGPLFFLSYGAANWLASRR
                     AGVGSLVFDWEHAIPFWPWTILPYWSIDLLYALPLFVCTTRRELD"
BASE COUNT          507 a         1589 c         1476 g          618 t
ORIGIN      
        1 gacgacgctg atcttcgccg ccggccatcc gcagccgctg acccgcgccg gcgaggcgct
       61 gctcgccgcg ctgcgcgaac gccatgcgcc cgaccccgga tggcagcgca gcgatccggc
      121 gctgctgctc tgtctcgcgc tctgcggggc tgcggccgtg cgcggtctcg tgcccggcgg
      181 cgtcggtcac gtgctggccc cgcctgccag cgacgacgat gcaccggtcg ggctcgatgc
      241 cgggctggat ccgggcggca gcggctgcga cagcgggggt gacggtggct gcggcagcgg
      301 gtgcagcgga tgaaccccga cccgcaccgg cccggcaccg cggccaacga tgccggaccg
      361 ccggccctga gcacgcccgc gggcgcggac tgcaccgcgg ccctgccgcc ggcctgccgc
      421 gacccggtcc tcgcggcgct gtaccagcgg ctgctcgcct acgaattcga cgatccgcag
      481 gcggcgctgc cgttcagccg gcgtctggcg cgcgacaacg gctggacgca tcgcggcgcg
      541 ctgcgggcga tcgacgaata ccggcgcttc gttttcctcg cgctcgccgc cggccatccg
      601 gtgacaccgt ccgaccaggt cgatcaggtc tggcacctgc acctcgtcta cacgcgcatg
      661 tactggctgg acttctgccc gcaggtcctc ggccgatccc tgcaccacgg cccgacccgc
      721 ggcggtgcgg ccgagggcgg gcgctacgac gagcagtacc ggcgcacgct ggcaagctac
      781 gaacgctgct tcggccaccc gccgccggcg gatctctggc cggcacccga actgcgcttc
      841 gggcgcgatc tcggtttcgt gcgcgtcaac cgccatgcct gctggctgct gccgcgcagc
      901 cccgaggcct ggcgcttcgt gctgcaccga cggctcgggc ggctgttgcg cagcttgcgc
      961 cggacgctgg cccgcttcgg ccttgcctct gaacccgcac caggtcctgt ccgcgacccc
     1021 gggagaaagt catgagcgca tcgttcctgc gccgccagca tccgggtcgc ggcgcctgcc
     1081 tcggcctgct gccggtgctg tgtctcgtca gcgtcagcga cagcgccggc gcactcgaca
     1141 acccgctcga ctacgacggc agccacttcc tcggactcta cgtgccgctg atgctggcga
     1201 gcctcgtgct ggccatgctg ctgcgccgct ggctgcgtct gcccggtccc gatccgcgcc
     1261 accagccccg gctcgatgtc tacgaaactg catatctcgc cggcggcgcc gggcgcacgg
     1321 tcgatacggc cctggccgtg atggcgctgc gcggcgagat cggcgccagc cccgacggcc
     1381 tgcgcctgca ggcctgcggc cggccgcagc tgcgcgaccc gatcgaacgc aaggtgttcg
     1441 gggccctgac gctggagggc cggccgccgg gcctgcaccg ccgtgtccgc cccgtgcttt
     1501 gcgccgaact cgatgcacgg ctgcgcccgc agggactgct gccgacgccg gtgcaggcgg
     1561 cgatggtccg gctgctgccg gccgcctgca ccggatcggt gctgctgctc gggctgctga
     1621 agctcgcgat cggtctgtca cggcatcgac cggtcggctt cctcgtcgtc tgctgcctcg
     1681 tgctcagcat cgcaacgctg gtgctgctgc tgaagaccgt gccgcgcagc cgccacggcg
     1741 acgccgtgct gaaacagctg cgcagcggcc tgtcggcccg caccgtcgaa tcgcggcagg
     1801 atccgcgcct cgccttggtc atggcactgt ttggcgtggc ggccgtcgcg catctgctgc
     1861 ccgaggacct cgccgaacaa ctggcgccgg ccggttccgg cggcggcgat tcgggcagtg
     1921 gatcggacgg cagcgacagc ggcggcgggg acagcggtgg cgacagcggc ggtggcggct
     1981 gcgggggctg tggcggcggc ggtggcgact gaagcgtgag cctgcgggct ggccccgggc
     2041 acaggccggc tgatccgcca ccgcacacac cggcattgca gcaagtccgg caaccggcag
     2101 ccatgcttgt cgggcgcggc cggcccccgc gcattccccc gccccgcccg acgacggagc
     2161 cctcgatgcg cgaaccccgg gaactgcact tcgacacgcg tgacggcagc gcgctgttct
     2221 accggcactg gccggccctc gacggccccg gcgagcaggc gctgctgctg ctgcaccgcg
     2281 gccacgagca ttcgggccgg ctgcagcacg tggtcgatga gctcgatctg ccgggtttcg
     2341 cggtgttcgc ctgggatgcc cgcggacacg gccgttcgcc ggggccacgc ggtcattgcc
     2401 ccggcatcgc caccgccacc ggcgatctcg acaccttcgt gcgccacctc cgcgaccggc
     2461 acggcatcgc gcccgaaaac ctcgtcgtga tcgcgcagag cgtcggtgcc gtgatcgccg
     2521 cgacctgggc gcacgactac gcgccgccgg tgcgcgcgct ggtgctcgcg gctccggcct
     2581 tcaaggtcaa gctgtacgtg ccgctggcgc gcccggcgct gcggctgcgt tacgcgctcg
     2641 gcggcaactt cgaggtgcag tcgtacgtga aggcgaagtt cctgacccac gatccggcgc
     2701 gcatcgccag ctacgacgcc gatccgctga tcacgcgcgc gatcgccgtc aacatgctgc
     2761 tcgggctgta cgaggccggc gagcgcgtgg tcgaggacgc cgcggcgatc acgctgccgg
     2821 tgctcgtgct ggtctcgggc gccgacttcg tcgtgcatcg ggcgccgcag cggcgcttct
     2881 tcgagcggct cggctcgaag gtgaaggcgt atcacgagct gccgggcttc tatcacgaca
     2941 cctttggcga actgcgccgc agcgaggcga tcgggctggt gcgcgccttc gtgcgcaagt
     3001 gcttcgatgc gccgttcgag cggccggacc tgcgcacggc cgaccgccac ggccatacct
     3061 tcgacgaata ccggcggctg caggcgccgc tgccggaagg ctcgtggaag attccgtact
     3121 acgcggcggt gcgcgccggc ctcgcgctcg gcgcccggct cgccgaatcg atccgcatcg
     3181 gtcacgagac cggcttcgac tcgggctcga cgctcgatgc cgtctaccgc aaccgcccgg
     3241 ccggcttcgg cccgcccgga cgactgatcg accggctgca cctcgattcg gccggctggg
     3301 ccggcatccg catccgcaag cagcacgtcg aggcgctgct cgccgatgcc gtgcgcgcgc
     3361 tgcgccgcgc gggccgcccg gtgcgcatcg tcgacatcgc cgccggccac ggccgctacg
     3421 tgctcgatgg cctcgcgacg ctgcccgagc cgctcgaagc ggcggattcg gtgctgctgc
     3481 gcgactacag cccgatcaac gtcgccgccg gccggcagct gatcgccgca cgcgggcggg
     3541 cggccacggt gcgcttcgag cagggcgacg ccttcgatcg cgacgcgctc gcagcactcg
     3601 cgccggcacc gaccgtcgcg atcgtctccg gccgctacga gctgtacccg gacaacgcgc
     3661 cgctggcaac gtcgctgtcg ggcctcgccg tggcgctcga agccggcggc ttcctgatct
     3721 acaccggcca gccctggcat ccgcagctcg aactgatcgc acgcgcgctg gcgagccacc
     3781 gcgacggcgc accgtgggtg atgcgccggc gcacgcaggg cgagctcgac cagctcgtcg
     3841 ccgcggccgg cttcgacaag tgcacgcagc tcatcgacga tgccggcatc ttcacggtgt
     3901 cgctggccgt gcggagggcg tcatgagcgc cgcaggaagt gccgccgggg tgcacgaagc
     3961 ccgcccgtgg ccgcgggcgc tgctctggct cgccgtcctc gggccgctgt tcttcctgag
     4021 ctacggcgcc gccaactggc tcgcgagccg gcgcgccggc gtcggcagcc tggtgttcga
     4081 ctgggaacac gcgatcccct tctggccgtg gacgatcctg ccgtactggt cgatcgacct
     4141 gctctacgcg ctgccgctgt tcgtctgcac gacgcggcgc gagctcgacg
//
LOCUS       DAOQJH010000154         4183 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_12926_length_4183_cov_4.541182, whole genome shotgun sequence.
ACCESSION   DAOQJH010000154 DAOQJH010000000
VERSION     DAOQJH010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 4183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4183
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12926_length_4183_cov_4.541182"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1231)
                     /gene="pheT"
                     /locus_tag="PLQ18_04780"
     CDS             complement(<1..1231)
                     /gene="pheT"
                     /locus_tag="PLQ18_04780"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020157697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HNE01002.1"
                     /translation="MKISEQWLREWTNPAVSTDELVAQLTMAGLEVDAVTPVAGVFSG
                     VVVGHVLDVQPHPDADKLRVCRVDAGSGEPLQIVCGAPNVHTGMRAPVATIGAVLPGD
                     FKIRKSKLRGVESFGMLCSARELGLSEDHGGLMPLPAEAVPGTDLRACLGLDDRVIEV
                     DLTPNRGDCLSVAGVAREVAVLNGSALTPVVCTPVPAASEEVFPVRIEAGADCPRYAG
                     RVIRGVDPRAATPLWMTERLRRAGLRSLGPLVDVTNYVLLELGQPMHAYDLDRLRQGI
                     HVRHANEGESLELLDGKSVELASDVLVIADGEGPVGLAGIMGGARTAVQPGSTRVFLE
                     VAYFAPSAIAGRARRFGLTTDASQRFERGVDPEGGRRAIERATALLLEIAGGRAGPVQ
                     VTEEPALLPSRSPVLLRR"
     gene            complement(1262..2278)
                     /gene="pheS"
                     /locus_tag="PLQ18_04785"
     CDS             complement(1262..2278)
                     /gene="pheS"
                     /locus_tag="PLQ18_04785"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008931333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HNE01003.1"
                     /translation="MDTLQTIVLAAETAVAAAADLAALDQVRVHYLGKKGVLTEQLKT
                     LGTLAPEARREAGAAINVAKDAVQAALEARRQALEAEALHRRLAAERVDVTLPGRGVQ
                     AGGLHPITRTLRRIEELFRQVGFSVAEGPEIEDDFHNFEALNIPAHHPARAMHDTFYF
                     DASTVLRTHTSPVQIRTMRACEPPIRIIAPGRVYRCDSDLTHSPMFHQVEGLFVDEDV
                     SFADLKGVLYDFLRAFFERDLDVRFRPSYFPFTEPSAEVDIQCVICGGSGCRVCKHTG
                     WLEVLGSGMVHPAVFGKVGIDPERYTGFAFGMGVERLAMLRYGVNDLRLFFDNDLRFL
                     GQFR"
     gene            complement(2442..2801)
                     /gene="rplT"
                     /locus_tag="PLQ18_04790"
     CDS             complement(2442..2801)
                     /gene="rplT"
                     /locus_tag="PLQ18_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003633276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HNE01004.1"
                     /translation="MPRVKRGVTAHARHKKILDKAKGYQGARSRVFRVAKQAVTKAAQ
                     YAYRDRRQKKRQFRALWIVRINAAARTCGLSYSRLIGGLKKAAIVIDRKVLADMAVFD
                     KAGFAAVAEKAKAALAQ"
     gene            complement(2834..3031)
                     /gene="rpmI"
                     /locus_tag="PLQ18_04795"
     CDS             complement(2834..3031)
                     /gene="rpmI"
                     /locus_tag="PLQ18_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015464854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HNE01005.1"
                     /translation="MPKMKTNRGAAKRFARTGSGGFKRSQAFARHILTKKTTKRKRQL
                     REPAMVAAVDVPMVRRMMPYA"
     gene            complement(3095..3613)
                     /gene="infC"
                     /locus_tag="PLQ18_04800"
     CDS             complement(3095..3613)
                     /gene="infC"
                     /locus_tag="PLQ18_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009116464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HNE01006.1"
                     /translation="MAANNEIRVNDEITAREVRLIDQNGEQAGVMPLFQAKRIAEDAE
                     LDLVEISPTAKPPVCRIMDYGKFRFEQQKKAAEARKKQKQIQVKEVKFRPGTDEGDYQ
                     VKLRNLVRFLTEGDKAKITLRFRGREVVHQDIGLNLLKRIEDDLAEFGTIEQRPKMEG
                     RQMIMVVAPKRK"
     gene            complement(3617..>4183)
                     /locus_tag="PLQ18_04805"
     CDS             complement(3617..>4183)
                     /locus_tag="PLQ18_04805"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017737823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HNE01007.1"
                     /translation="LPGEGAFYGPKVEYHIRDAIGRSWQCGTLQLDFVLPGRLGAEYV
                     AEDNTRKVPVMLHRAVLGSMERFIGILIEHHAGQMPTWLAPLQAVVLNITDRAAEAAT
                     AAAARLNALGHRAEADLRNEKIGFKIREHTLRRIPYLLVIGDREVETGSVAVRTRTGK
                     DLGVMTLGSFEEFLAADVASRGRSVLEG"
BASE COUNT          663 a         1418 c         1413 g          689 t
ORIGIN      
        1 cccgccgcag caggacgggg gacctggatg gcagcaacgc cggttcctcg gtgacctgca
       61 cgggccctgc ccgtccaccg gcgatctcga gcagcagcgc cgtcgcacgc tcgatcgcgc
      121 gccgcccgcc ctcgggatcg acaccgcgct cgaagcgctg gctggcatcc gtcgtgaggc
      181 cgaaacgcct cgcgcggccg gcaatggcgg acggcgcgaa gtacgcgact tcgaggaaca
      241 cgcgcgtgct gcccggctga accgctgtcc gggctccacc catgatgccg gcgaggccaa
      301 ccggcccctc cccgtcggcg atcaccagca cgtcggaagc gagttccacg gacttaccgt
      361 ccagcagctc gagcgactcg ccctcgttgg catgacgcac gtggatgcct tgacgcaggc
      421 gatccaggtc gtaggcatgc atcggctggc cgagttcgag cagcacgtag ttggtgacat
      481 cgaccagcgg cccgagcgag cgcaggccgg cgcggcgcag gcgctcggtc atccacagcg
      541 gcgtcgcggc ccgcggatcg acaccacgga tcacgcgccc ggcgtagcgc gggcagtcgg
      601 ccccggcctc gatgcgcacc gggaaaacct cttcgcttgc ggccggcacc ggcgtgcaga
      661 cgaccggcgt cagggccgag ccgttcagca ccgcgacctc gcgtgcgacg ccggcgacac
      721 tcaggcagtc gccgcggttc ggggtcaggt cgacctcgat gacccggtcg tcgaggccga
      781 ggcaggcgcg cagatcggtg ccgggcacgg cttcggccgg cagcggcatc aggccgccgt
      841 ggtcttcgga caggccgagt tcgcgcgccg agcagagcat gccgaaggac tcgacgccgc
      901 gcagcttgct cttcctgatt ttgaaatcac cgggcagcac ggcgccgatg gtcgcgaccg
      961 gcgcgcgcat gccggtgtgc acgttcggcg cgccgcagac gatctgcagc ggctcgccgc
     1021 tgccggcatc gacgcggcag acgcgcagct tgtcggcatc ggggtgcggc tgcacgtcga
     1081 gcacgtggcc gacgacgaca ccgctgaaca ccccggccac cggcgtgacg gcatcgacct
     1141 ccagaccggc catcgtcagc tgcgcgacca gctcgtcggt cgacacggcg ggattcgtcc
     1201 actcccgcag ccactgttca ctgattttca tgggttttcg gcctccgggc ggcgcacggc
     1261 ttcagcggaa ctggccgagg aagcgcagat cgttgtcgaa gaacaggcgc aggtcgttga
     1321 cgccgtagcg cagcatcgcc agacgctcga cgcccatgcc gaaggcgaaa ccggtgtagc
     1381 gctccgggtc gatgccgacc ttcccgaaca cggccggatg caccatgccg ctgccgagca
     1441 cctccagcca gccggtgtgc ttgcagaccc ggcagccgct gccgccgcag atcacgcact
     1501 ggatgtcgac ctcggccgag ggctcggtga acgggaagta cgaggggcgg aagcgcacgt
     1561 cgaggtcgcg ctcgaagaag gcgcgcagga agtcgtagag cacgcccttg aggtcggcga
     1621 agctcacgtc ctcatcgacg aacaggccct cgacctggtg gaacatcggc gaatgcgtca
     1681 ggtcgctgtc gcagcggtag acgcgcccgg gcgcgatgat gcggatcggc ggctcgcagg
     1741 cgcgcatcgt gcggatctgc accggcgagg tgtgcgtgcg cagcaccgtc gaggcgtcga
     1801 aatagaaggt gtcgtgcatc gcgcgcgccg ggtggtgcgc gggaatgttc agcgcctcga
     1861 agttgtggaa gtcgtcctcg atctccgggc cttcggcgac cgagaagccg acctgacgga
     1921 acagctcctc gatgcggcgc agcgtgcgcg tgatcggatg caggccgccg gcctgcacgc
     1981 cgcgccccgg cagcgtcacg tcgacgcgct cggcggcgag gcgccggtgc agggcctcgg
     2041 cttcgagcgc ctggcggcgc gcttcgagcg cggcctgcac ggcgtccttg gcgacgttga
     2101 tcgcggcacc ggcctcgcgc cgcgcctcgg gcgcgagcgt gccgagcgtc ttcagctgtt
     2161 cggtcagcac gcccttcttg ccgaggtaat gcacgcgcac ctgatcgagc gcggcaaggt
     2221 cggcagcggc ggcgacggcg gtttcggccg cgagcacgat ggtctgcagg gtatccacgg
     2281 ggcagctccg ttcgggggga aaccgcggcg gtgcgcgcac gcgcccggct ccgcgttccg
     2341 gcgatgctca acaagcaaca aaaaagggaa aggagctcaa gacccccttc cctttcgcga
     2401 tccgtccgcc gtcacgcaga cggcgacggc agctttttcg gtcactgcgc cagggcggcc
     2461 ttggcctttt ccgcgacggc ggcaaaaccg gccttgtcga acaccgccat gtcggcgagg
     2521 accttgcggt cgatgacgat cgcggccttc ttcaggccac cgatcagccg gctgtacgac
     2581 aggccgcagg tgcgggccgc ggcgttgatg cgcacgatcc acagcgcgcg gaactggcgc
     2641 ttcttctggc gacggtcgcg gtacgcgtac tgggcggcct tggtgacggc ctgcttggca
     2701 acgcggaaca cgcgggaacg ggcaccctgg tagcccttgg ccttgtccag gatcttcttg
     2761 tgacgggcgt gggcggtaac gccgcgcttc actcggggca tggtctttgt cctctagtcc
     2821 ggatcgtgac gactcaggcg tacggcatca tgcgacgcac catcggcacg tcgacggccg
     2881 cgaccatcgc cggctcgcgc agctggcgct tacgcttcgt cgtcttcttc gtcaggatgt
     2941 gacgggcaaa ggcctgggaa cgcttgaaac caccggaacc ggtgcgcgcg aaacgcttcg
     3001 cggcgccgcg gttcgtcttc atcttgggca tgtcagccac tccgcattca ttgattgatt
     3061 gcggactgca accgcgcatc ggccagaagc ggaatcactt ccgcttcggc gcaacgacca
     3121 tgatcatctg gcggccttcc attttcggcc gctgttcgat cgtgccgaat tcggcgaggt
     3181 cgtcctcgat gcgcttcagg aggttcagtc cgatgtcctg gtgcacgacc tcacgcccgc
     3241 ggaagcgcag cgtgatcttg gccttgtcac cttcggtcag gaaacgtacc aggttgcgta
     3301 gtttgacctg gtaatcccct tcgtccgtcc cgggacggaa tttcacttcc ttgacctgaa
     3361 tctgtttctg cttcttgcgg gcttccgctg ccttcttctg ctgctcgaaa cggaatttgc
     3421 cgtaatccat gatccggcag accggcggtt ttgccgtcgg cgagatttcg accagatcga
     3481 gttcagcgtc ttcggcgatc cgcttggcct ggaagagcgg catcacaccc gcctgttcgc
     3541 cgttctgatc gatcagacgc acttcgcggg cggtgatctc gtcgttcacc ctaatttcgt
     3601 tgttcgcagc gatgagtcag ccctccaaaa cagaacggcc gcggctcgcg acatcggcag
     3661 cgaggaattc ctcgaaggag ccgagcgtca tgacgcccag atccttgccg gtccgcgtac
     3721 gcacggccac agaacccgtt tccacctcac gatcgccgat caccagcaga tacggaatcc
     3781 ggcgcagggt gtgttcgcga atcttaaagc cgatcttctc gttcctcaag tcggcctcgg
     3841 cacggtggcc gagcgcattg aggcgcgcag cggcagccgt cgcggcttcg gcggcgcggt
     3901 cggtgatgtt cagcaccacg gcctgcagcg gcgcgagcca ggtcggcatc tggccggcgt
     3961 ggtgctcgat caggatgccg atgaagcgct ccatcgagcc gagcaccgcg cggtgcagca
     4021 tgaccggcac cttgcgcgtg ttgtcctcgg cgacgtactc ggcgccgagg cggcccggca
     4081 gcacgaagtc gagctgcagc gtgccgcact gccaggagcg gccgatcgcg tcgcggatgt
     4141 ggtactcgac cttcggcccg tagaaggcgc cctcacccgg cag
//
LOCUS       DAOQJH010000155         4182 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_12930_length_4182_cov_3.814878, whole genome shotgun sequence.
ACCESSION   DAOQJH010000155 DAOQJH010000000
VERSION     DAOQJH010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 4182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4182
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12930_length_4182_cov_3.814878"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..251
                     /locus_tag="PLQ18_04810"
     CDS             <1..251
                     /locus_tag="PLQ18_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015006822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNE01008.1"
                     /translation="IDLAKGRQVYNTVCVICHQQGVAGAPKLGDKAGWASRLAQGFPV
                     LLEHAIKGYRLMPARGGNGQLADEDVANALGYIVSESQ"
     gene            complement(372..701)
                     /locus_tag="PLQ18_04815"
     CDS             complement(372..701)
                     /locus_tag="PLQ18_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017938974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE01009.1"
                     /translation="MNPAALEAMIARGQDGALLRTTLGKLYLDTGDAARAHAHLERAV
                     GLDAQYSAAWKLLGHARAALGDPAGALAAWTQGIAVAEARGDLQAAKEMAVFHRRLLR
                     SLPPDAP"
     gene            complement(698..1270)
                     /locus_tag="PLQ18_04820"
     CDS             complement(698..1270)
                     /locus_tag="PLQ18_04820"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018233236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNE01010.1"
                     /translation="MPVTDRDRWDARYRDGIPQQRPCATLTAHLHLLPARGRALDLAC
                     GLGANARVLAAVGLDTEAWDVSPVAIGRLQALAAAEGLRITATACDLLETPPAPQSFD
                     VIVVAYFFERALLPALAAALRPGGLLFYETFTQRSAHGPSNPDWKLADNELPSALPGL
                     RLRYYRDEQDAGDPALGLRGIAGYIGQRPA"
     gene            complement(1290..2666)
                     /gene="trkA"
                     /locus_tag="PLQ18_04825"
     CDS             complement(1290..2666)
                     /gene="trkA"
                     /locus_tag="PLQ18_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013034686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HNE01011.1"
                     /translation="MKILILGAGQVGSSVAYNLASEDNDITVVDLRADVLADLQDRID
                     IRTVCGACSHPEILEAAGAADADMLIALTSSDEVNMIACAVADSLFRTPTKIARVRTA
                     EYTQYPQLFGAHGLPIEKLIAPEQLVTDHIQRLIEHPGALQVLDFADGLVRLVGVRAI
                     AGGTLVGKQLRTLPAHMPGIHARVAAIFRAGEAITPDGDTVIEADDEVFFIAAKKSIR
                     AVMAELRRLDKPVKRVILAGGGNIGRRLAHALEDRFQVKIIERNPEVARRIAEQLDRT
                     IVLAGDAADEELLLEENIEHTDVFCAVTNDDEANILSSMLAKRLGARKVMALINRPAY
                     VDLVEATGQIDVAISPQQATIGALLTHIRRGDVVRVHALRRGAAEAIEAIAHGDRQTS
                     KVVGRRIDEIRLPPGTTIGAVVRNGEVTICHHDTLIEPDDHVILFLTDKKRIHEVERL
                     FQVAVTFL"
     gene            complement(2717..4090)
                     /locus_tag="PLQ18_04830"
     CDS             complement(2717..4090)
                     /locus_tag="PLQ18_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018879671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNE01012.1"
                     /translation="MSSAHILVVDDEPDIRDLVKEILEDENYEVSTAENGAAAREARR
                     ARRPDLILLDIWMPDTDGISLLKEWAEGDGLPCPVIMMSGHGTVETAVEATRLGAWDF
                     IEKPLSMAKLLLTLERALEADKLRRENAGLKRHARPVTEPVGRSETVQKLKAQAQRIA
                     QHEAPVLISGEPGTGKEIYARYVHAHSPRRGGPFVDVGVGSIARENSALELFGSEEGE
                     RLHYGRLEQASGGTLFLDELADMAPETQAKLAGALENGSFLRLGGREPVHLNVRVIAA
                     THRDLESEVAAGRFREDLYYHLNVLPIRLPPLREHCEDVPDLLSFYVNHFVDQESLPY
                     RNFTMAARNRLRNYGWPGNIRELKNLVQRLLILGGAEEITLEEVESAISHTTPGRQRD
                     ISQIPLDLPLREAREQFERAYLLQQFKECDGNVARLAEKVGMERTNLYRKLRALGIDP
                     KRTPEDE"
BASE COUNT          559 a         1370 c         1579 g          674 t
ORIGIN      
        1 acatcgatct ggccaagggc aggcaggtct acaacaccgt ctgcgtgatc tgccaccagc
       61 agggcgtggc cggtgcgccg aagctcggcg acaaggcggg ctgggcgtcg cggctggcgc
      121 agggcttccc ggtgctgctg gagcacgcga tcaagggtta ccggctgatg ccggcgcgcg
      181 gcggcaacgg ccagctcgcc gacgaggacg tggcgaacgc gctcggctac atcgtcagcg
      241 aatcgcagtg agatcgcagt gagccggtga ccggcacgct ccggtccacc cacggccaca
      301 cacagggcgc ctccgggcgc cctgtgcttt tgtggagggt cgtgcggcgg gctgaggacg
      361 cgggcaggaa gctacggggc gtccggtggc aggctgcgca gcaggcgacg gtggaacacc
      421 gccatctcct tcgcggcctg caggtcgccg cgggcttcgg cgaccgcgat gccctgcgtc
      481 catgccgcaa gcgcaccggc cgggtcgccg agtgcggcgc gggcgtggcc gagcagcttc
      541 caggcggccg agtactgcgc atcgagcccg accgcgcgtt cgaggtgggc atgtgcgcgc
      601 gccgcgtcgc cggtgtcgag gtacagcttg ccgagcgtcg tgcgcagcag ggcgccgtcc
      661 tggccgcgcg cgatcatggc ttcgagggcg gcgggattca tgccgggcgc tggccgatgt
      721 agccggcgat gccgcgcagg ccgagcgccg ggtcgccggc gtcctgctcg tcgcggtaat
      781 agcgcaggcg caggccgggc agggccgatg gcagttcgtt gtcggcgagc ttccagtcgg
      841 ggttcgacgg gccgtgggcg ctgcgctgcg tgaaggtctc gtaaaacagc aggccgccgg
      901 ggcgcagcgc cgcggcgagt gccggcagca gcgcgcgctc gaagaagtac gcgacgacga
      961 tcacgtcgaa gctctgcgga gccgggggcg tttccagcag gtcgcaggcc gtggcggtga
     1021 tcctcaggcc ttcggcggcg gcgagtgcct gcagccgccc gatcgcgacc ggcgacacgt
     1081 cccaagcctc ggtgtcgagc ccgaccgcgg ccagcactcg ggcgttggca ccgaggccgc
     1141 aggccagatc cagcgcccgg ccacgggccg gcagcaggtg caggtgtgcg gtcagcgtcg
     1201 cgcaggggcg ctgctgcggg atgccgtcgc ggtaacgggc atcccagcga tcgcggtcgg
     1261 tgacgggcat gcggggttcc cggggtggct cagaggaagg tcacggcgac ctggaacagg
     1321 cgctcgacct cgtggatgcg cttcttgtcg gtcaggaaca ggatcacgtg atcgtcgggt
     1381 tcgatcagcg tgtcgtggtg gcagatggtg acctcgccgt tgcgcacgac ggcgccgatg
     1441 gtcgtgccgg gcggcaggcg gatctcgtcg atgcgccggc cgacgacctt gctggtctgg
     1501 cggtcgccgt gggcgatggc ctcgatggct tcggccgcac cgcggcgcag cgcatgcacg
     1561 cgcacgacat cgccgcggcg gatgtgcgtc agcagcgcac cgatcgtcgc ctgctgcggc
     1621 gagatcgcca cgtcgatctg gccggtggcc tcgaccaggt cgacgtaggc cgggcggttg
     1681 atcagcgcca tgaccttgcg ggcgccgagg cgcttggcga gcatcgacga caggatgttg
     1741 gcctcgtcgt cattggtcac ggcgcagaac acgtcggtgt gctcgatgtt ctcttcgagc
     1801 agcagctcct cgtcggcggc atcgccggcg agcacgatcg tgcggtcgag ctgttcggcg
     1861 atgcggcgtg cgacctcggg gttgcgctcg atgatcttga cctggaagcg gtcctccagc
     1921 gcgtgcgcga gccgccggcc gatgttgccg ccgccggcga ggatcacgcg cttgaccggc
     1981 ttgtcgagcc tgcgcagctc cgccatcacc gcgcggatgc tcttcttcgc ggcgatgaag
     2041 aacacctcgt cgtcggcctc gatcaccgtg tcgccgtcgg gcgtgatcgc ctcgccggcg
     2101 cggaagatcg ccgcgacgcg cgcgtggatg ccgggcatgt gtgccggcag cgtgcgcagc
     2161 tgcttgccga ccagcgtgcc gccggcaatc gcgcgcacgc cgaccagacg cacgaggccg
     2221 tcggcgaagt ccagcacctg cagcgcgccg gggtgctcga tcaggcgctg gatgtggtcg
     2281 gtgacgagct gctcgggcgc gatcagcttc tcgatcggca ggccgtgcgc gccgaacagc
     2341 tgcgggtact gcgtgtactc ggcggtgcgc acgcgcgcga tcttggtcgg cgtgcggaac
     2401 aggctgtcgg cgaccgcgca ggcgatcatg ttgacctcgt cgctgctggt cagcgcgatc
     2461 agcatgtcgg catcggcggc tccggcggct tcgaggatct ccgggtgcga acaggcgccg
     2521 cagacggtgc ggatgtcgat gcggtcctgc aggtcggcga gcacgtcggc gcgcaggtcg
     2581 acgacggtga tgtcgttgtc ctcggaagcg aggttgtacg cgaccgagct gccgacctgg
     2641 ccggcaccga ggatcaggat cttcacgatg tcctcacgga atcctcacga cggcaccggg
     2701 ctgcttcgtg cccggctcat tcgtcctcgg gcgtgcgctt cggatcgatg ccgagggcgc
     2761 gcagcttgcg gtacaggttc gtgcgctcca tgccgacctt ctcggcgagg cgcgcgacgt
     2821 tgccgtcgca ttccttgaac tgctgcagca ggtaggcgcg ctcgaactgc tcgcgcgcct
     2881 cgcgcagcgg caggtcgagc ggaatctggc tgatgtcgcg ctggcgcccg ggcgtcgtgt
     2941 gcgagatcgc cgattcgacc tcttcgaggg tgatctcctc ggcgccgccg aggatcagca
     3001 ggcgctgcac caggttcttc agttcgcgga tgttgccggg ccagccgtag ttgcgcaggc
     3061 ggttgcgcgc cgccatcgtg aagttgcggt acggcaggct ttcctgatcg acaaaatgat
     3121 tgacgtagaa cgacagcagg tcgggcacgt cctcgcagtg ctcgcgcagc ggcggcagcc
     3181 ggatcggcag cacgttcagg tggtagtaga gatcctcgcg gaagcgcccg gccgcgacct
     3241 cggattcgag gtcgcgatgc gtcgcggcga tcacgcgcac gttcaggtgc accggctcgc
     3301 ggccgccgag gcgcaggaag gagccgttct cgagcgcgcc ggcgagcttg gcctgggtct
     3361 cgggcgccat gtcggcgagc tcgtcgagga acagcgtgcc gccgctcgcc tgttcgagcc
     3421 ggccgtaatg caggcgctcg ccttcctcgg agccgaacag ctccagcgcc gagttctcgc
     3481 gcgcgatcga gccgacgccg acatcgacga acggcccgcc gcggcgcggg ctgtgcgcat
     3541 gcacgtagcg cgcgtagatc tccttgccgg tgccgggctc gccggagatc agcaccggtg
     3601 cctcgtgctg ggcgatgcgc tgggcctggg ccttgagctt ctgcacggtc tcgctgcgcc
     3661 cgaccggctc ggtcaccggc cgcgcgtggc gcttgaggcc ggcgttctcg cggcgcagct
     3721 tgtcggcttc gagcgcgcgt tcgagcgtca gcagcagctt ggccatcgac agcggcttct
     3781 cgatgaagtc ccaggcgccg aggcgcgtgg cctcgaccgc ggtctcgacc gtgccgtggc
     3841 cggacatcat gatcaccggg cagggcaggc cgtcgccctc ggcccattcc ttgagcaggc
     3901 tgatgccgtc ggtatccggc atccagatgt cgagcaggat caggtccggg cgccgcgcac
     3961 ggcgggcctc gcgggcggcg gcgccgtttt ccgcggtcga gacctcgtag ttctcgtctt
     4021 cgaggatttc cttgaccaga tcgcggatgt cgggctcgtc gtcgacgacg aggatgtgcg
     4081 ctgaactcat cctgcctcct cggcacgggt cgatggcacg gcacccggcg ccggtgcggc
     4141 ggcaggtgcg ggtgtcagtg gggtgcgggc gtgactggcg gg
//
LOCUS       DAOQJH010000156         4182 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_12932_length_4182_cov_3.109038, whole genome shotgun sequence.
ACCESSION   DAOQJH010000156 DAOQJH010000000
VERSION     DAOQJH010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 4182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4182
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12932_length_4182_cov_3.109038"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            33..203
                     /locus_tag="PLQ18_04835"
     CDS             33..203
                     /locus_tag="PLQ18_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HNE01013.1"
                     /translation="MNCGHCVRAIETAVRALDARAEVRCDLDAKTVQVATGLAGAAVL
                     EAIREAGYTPST"
     gene            complement(185..1966)
                     /locus_tag="PLQ18_04840"
     CDS             complement(185..1966)
                     /locus_tag="PLQ18_04840"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNE01014.1"
                     /translation="MFRTSSESPASGNPGPRADARRRLLRTLLSLLLLWGLVLIAVLQ
                     LRALQGEHADIRLQRISTAVAAETGRRFEAIGTLLGAAQAWATQPEDTRPNAQSPYPL
                     LTSGCRRELGAGCRLLRLDAEGRLQPLHDDSGTILPARPLPLPAPGVALIAHLEAVTA
                     ATGTDLLLYGVAATDSGAAVRAVYAAVPVRDFIDAYATLPPAGEARIELLHADGSPLL
                     EILRADAVAAAGYSRIVALTPLPLRLHVSLPAPPLYQDWRLAAALLIAAAGLYSGRRL
                     LGLRQAAGKPGDGVEPDTLLALRQSQRLGRGGHWVYDARLRTLKLSDELRALLSEALA
                     ANLQPLAAGDDVTPAPGTEQLHSDLQRAIVNECEFAGEYRVSDARDQPQWLSVHADAS
                     YNSEGRYLGHIAYVQDITERKQLELELRRLATTDSLTGILNRGSFLDRTAEEIARARR
                     YGRPLSLIVLDLDDFKTINDRFGHPVGDQCLRAFAETGTACLRRADRLGRLGGEEFAA
                     LLPETGLPAALGAAERIREHVQRLRVPLGDGLDTGFTVSAGVTLLSDHHQSIEQLLAA
                     ADRALYRAKAEGRNRVCLDNDLQVEGV"
     gene            complement(2249..3397)
                     /locus_tag="PLQ18_04845"
     CDS             complement(2249..3397)
                     /locus_tag="PLQ18_04845"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HNE01015.1"
                     /translation="MNDPASALSAAELQRALGELQRPAAGTAGFATDAEAIAGFLGEF
                     ADVPHTQRSYRKEVVRFHLWCRLQRQRDLAGITRADVQAWEGFLAAPPPEWIGPRHAR
                     LGSPGWRPFEGPLTAASRRQAAVILGGFYGYLQRCRYLDYNPFLVMRRRAGARTDATA
                     PMRVLSRETLERVLQVLGSRADAAGDARRRDEAERRLFTVRFLVNAGLRRAELARAHM
                     SDLLQREDGDGRPAQWFLSVCGKGGRLRQVALNEAALQALGRWRSHLQATHGLDAQQG
                     ALLVPWKRAGERAAAGVSEAVVYRLVKAALADAGRVLAAEFPADAALLARASPHWLRH
                     SYATLTANADVPLELVRDQLGHASLDTTLLYRHREDLERARRLRHVAL"
     gene            complement(3394..>4182)
                     /locus_tag="PLQ18_04850"
     CDS             complement(3394..>4182)
                     /locus_tag="PLQ18_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011384991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE01016.1"
                     /translation="NLTIDGSYAMDDAALEAGKSFAGQDQQAVMAALAEDPQALIDLV
                     NGTDFDLTVSYTMNQELADFASANAGVEIPASGSMGAIMVDGVLYADLSEFAALGAPE
                     GWIGVPVAEALQAQVDVWRRVRGLTPKALAQQIADDRIDILLDLSGHTGGNRLMTFAR
                     KPAPVQATRTRRPVAPRRRPGASASPSPPAIPQALPAPATMRSPPPATAGASAAAVVV
                     PVAATRSRSPPAKPGVTPGVTTTTATTSAMPEAPATGLRTPRGR"
BASE COUNT          587 a         1427 c         1570 g          598 t
ORIGIN      
        1 tgaacgagac gatcaccctg aaggtgcccg acatgaactg cggccattgc gtgcgcgcaa
       61 tcgagaccgc cgtgcgcgcc ctcgatgcgc gcgccgaggt ccgctgcgat ctcgatgcga
      121 agacggtgca ggttgccacg gggcttgccg gggcagccgt gctcgaagcg atccgcgagg
      181 ccggctacac gccctcgacc tgaaggtcgt tgtcgaggca gacgcggttg cggccttcgg
      241 ccttggcgcg gtacagcgcc cggtcggccg ccgccagcag ctgctcgatg ctctggtggt
      301 gatcggacag cagcgtgacg ccggcgctga ccgtgaagcc ggtgtcgagg ccatcgccga
      361 gcggcacgcg cagccgctgc acgtgctcgc ggatgcgctc ggcggccccg agggcggccg
      421 gcagaccggt ctcgggcagc agtgctgcga actcctcgcc gccgagccgc ccgaggcggt
      481 cggcgcggcg caggcaggcc gtgccggttt cggcgaaggc ccgcaggcac tggtcaccga
      541 ccggatggcc gaagcggtca ttgatggtct tgaagtcgtc gagatcgagc acgatcagcg
      601 acagcggccg accgtagcgg cgggcgcggg cgatctcctc ggcggtgcgg tcgaggaagc
      661 tgccgcggtt caggatgccg gtcagggaat cggtggtcgc gagccgccgc agttccagtt
      721 cgagctgctt gcgttcggtg atgtcctgca cgtaggcgat gtggccgaga tagcggcctt
      781 cgctgttgta gctcgcatcg gcatgcacgg acagccactg cggctggtca cgggcatcgc
      841 tgacccggta ctcgccggcg aactcgcatt cgttgacgat cgcgcgctgc aggtccgagt
      901 gcagctgctc ggtgccgggc gccggcgtca cgtcgtcccc ggcggcgagc ggctgcaggt
      961 ttgcggcgag ggcttccgag agcagcgcgc gcagctcgtc ggacaacttc agcgtgcgca
     1021 ggcgcgcgtc atagacccag tgtccgccgc ggccgaggcg ctgggactgg cgcagcgcca
     1081 gcagcgtgtc cggctcgacg ccgtcaccgg gttttccggc cgcttgccgc agcccgagca
     1141 gccgccgacc gctgtagagc ccggcggccg cgatcagcag ggcggctgcc aggcgccagt
     1201 cctggtaaag gggcggagcc ggcaggctga cgtgcaggcg cagcggcagc ggcgtcagcg
     1261 cgacgatccg gctgtagccg gcagccgcca cggcatcggc ccgcaggatt tcgagcagcg
     1321 ggctgccgtc ggcatgcagc agttcgatgc gcgcctcgcc ggcgggcggc agggtcgcat
     1381 aggcatcgat gaaatcgcgc accggcacgg cggcatacac ggcccgcacg gccgcgccgc
     1441 tgtcggtggc cgcgacgccg tagagcagca gatcggtgcc ggtggcggcg gtgacggcct
     1501 ccaggtgtgc gatcagcgcc acgccggggg ccggcagcgg cagcggccgg gcaggcagga
     1561 tcgtgccgct gtcgtcgtgc agcggctgca gacggccctc tgcatcgagc cgcagcagcc
     1621 ggcagccggc gccgagttcg cgccggcagc cgctggtcag cagcggatag ggcgattgcg
     1681 cgttcgggcg ggtgtcctcg ggctgcgtcg cccaggcttg cgcggcgccg agcaaggtgc
     1741 cgatggcttc gaaacggcgg ccggtttcag cggcgacggc ggtgctgatg cgctgcaggc
     1801 ggatgtcggc gtgttcgccc tgcagggcac gcagctgcag cacggcgatg aggaccaggc
     1861 cccagagcag cagcagcgac agcagggtgc gcagcaggcg ccggcgggca tcggcgcgcg
     1921 ggccgggatt gcccgaggcc ggagactccg acgaagtccg gaacatgagc agccatctcg
     1981 tcagcggtgc cggcatcagc aagatccctg tgcgtgcggg gtggtgatcc ttcggaggtc
     2041 gggccggcaa ggcatccggt gacgcaggtt atcgattttg cacgggtggc gcacggccat
     2101 gtgcaagcat gcttgccata aaggcaaacg ttaaattttg taaggtttat cacgtcttgc
     2161 ccgcacagtc catgcgtcgg gacagacacg ggacatggca cggatcagcg cagcggcggg
     2221 cgattccggg cgcggagggg gatgcgcctc agagcgcgac gtggcgcagc cgccgggcgc
     2281 gctcgagatc ctcgcggtgg cggtacagca gcgtggtgtc gagcgaggca tggccgagct
     2341 ggtcgcgcac cagttccagc ggcacgtcgg cgttggcggt cagggtcgcg taactgtgcc
     2401 gcagccagtg cggactcgcc cgggccagca gcgcggcatc ggccgggaat tccgccgcca
     2461 gcacccggcc tgcgtcggcg agcgcggcct tgaccagccg gtagacgacg gcctcgctta
     2521 ccccggccgc tgcccgctcg ccggcgcgtt tccagggcac gagcagcgca ccctgctgcg
     2581 catcgagccc atgcgtggcc tgcagatgac tgcgccagcg cccgagcgcc tggagcgcgg
     2641 cctcgttgag cgcgacctgc cgcagccgcc cgcccttgcc gcagacgctc aggaaccact
     2701 gcgcgggccg gccgtcgcca tcctcgcgct gcagcaggtc gctcatgtgc gcgcgcgcga
     2761 gttcggcgcg gcgcaggccg gcgttgacga ggaagcgcac cgtgaacagc cggcgttcgg
     2821 cctcgtcgcg gcggcgcgcg tcgccggcag cgtcggcgcg gctgccgagc acctgcagga
     2881 cccgttcgag ggtttcgcgc gacagcacgc gcatcggcgc ggtggcatcc gtgcgggcgc
     2941 cggcgcgccg gcgcatgacc aggaaggggt tgtagtcgag atagcggcag cgctgcagat
     3001 agccgtagaa gccgccgagg atcaccgccg cctgccggcg cgaggccgcc gtcagcggcc
     3061 cttcgaacgg ccgccagccc gggctgccga gccgggcatg gcgcgggccg atccactcgg
     3121 gcggcggtgc ggccagaaag ccttcccagg cctgcacgtc ggcgcgcgtg atgccggcca
     3181 ggtcgcgctg ccgctgcagc cggcaccaga ggtggaagcg cacgacttcc ttgcggtagc
     3241 tgcgctgcgt gtgcggcaca tcggcgaatt cgccgagaaa gccggcgatg gcttcggcat
     3301 cggtcgcgaa ccccgccgtg ccggcggccg ggcgctgcag ctcgccgagc gcgcgctgca
     3361 gttcggccgc ggacagggcg cttgccgggt cgttcatcgc ccgcgcggcg tgcgcaggcc
     3421 ggtcgccggg gcctcaggca ttgccgaggt tgtcgccgtc gtcgtcgtca cgcccggcgt
     3481 cacgcccggc ttcgccggcg gcgagcggct gcgcgttgcc gccaccggca ccaccaccgc
     3541 cgccgcgctt gcgccggcgg ttgccggcgg cgggctgcgc attgttgccg gcgctggcag
     3601 ggcctgcggg attgccggcg ggcttgggct tgcgcttgcg cccggccggc gacgcggcgc
     3661 caccggcctg cgggttcggg ttgcctgcac cggcgccggt ttgcgggcaa acgtcatcag
     3721 ccggttgccg ccggtgtggc cagatagatc cagcaagatg tcaatgcggt cgtcggcaat
     3781 ctgctgcgcc aacgctttgg gcgtcagccc gcgcactcgc cgccagacat ccacctgcgc
     3841 ttgcagcgcc tcggcgaccg gcacgccaat ccagccttcg ggagcaccca gggcggcaaa
     3901 ctcgctgagg tcggcataca gcacgccgtc caccatgatc gcgcccatcg agccgctggc
     3961 gggaatttcc acgcccgcat tggcgctggc gaagtcggcc agttcctgat tcatcgtgta
     4021 gctgacggtc aggtcaaaat cagtgccgtt caccaggtcg atcagtgcct gcgggtcctc
     4081 tgcaagagca gccatcaccg cctgctggtc ctggccggca aaggacttgc ccgcttccaa
     4141 agccgcgtcg tccatggcat aggagccatc gatggtgaga tt
//
LOCUS       DAOQJH010000157         4175 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_12954_length_4175_cov_4.425000, whole genome shotgun sequence.
ACCESSION   DAOQJH010000157 DAOQJH010000000
VERSION     DAOQJH010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 4175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4175
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12954_length_4175_cov_4.425000"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..663)
                     /locus_tag="PLQ18_04855"
     CDS             complement(<1..663)
                     /locus_tag="PLQ18_04855"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010596520.1"
                     /note="catalyzes the formation of malate from glyoxylate
                     and acetyl-CoA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HNE01017.1"
                     /translation="MTERVAIGGLQVAKPLYDLVQNTLLPGTGLDAAGWWTALEAIVT
                     DLAPRNRELLAKRDALQAQIDAWHVARRGQPHDHAAYVAFLREIGYLLPEGPDFQVST
                     DNVDPEIASVAGPQLVVPVNNARYALNAANARWGSLYDALYGTDVLPEDGGAEKGKGY
                     NPARGDKVIAYAAGFLDQAVPLASGKHAEVTEYLRDGATLVAQLADGTKTALADPSAF
                     VGY"
     gene            1059..2441
                     /locus_tag="PLQ18_04860"
     CDS             1059..2441
                     /locus_tag="PLQ18_04860"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HNE01018.1"
                     /translation="MVVEAVLRTLDWLAIAMAGWLAFMLRFDAAVMPGPFQRAMIIAL
                     LVSIGVFGRCSVYRAWRGETLNGELRTLLWAWCLTFLITIGALALTTGRDEAPFPRLW
                     LMLWFVLGCCLLTLLRGCLRQVLRRLRSSGHNTRRVVVVVAGELGVSVIRRLCEARWT
                     ELRVVGYFDDRPIERVGEIAGVPRIGRIDELAAYVEGGHADQVWITLPFRAEERVREI
                     LETLRHATADIRMVPDILGLQLLNHSMGTVAGIPVINLSSTPMVGFNRVIKALEDRMI
                     SLAILLMIAPLMIAIAIGIKLSSPGPVFFRQERLGYDGRPFRVWKFRSMKVHQEHGGQ
                     VTQATRGDSRITPFGAFLRRTSLDELPQFFNVLDGSMSIVGPRPHAVAHNQEYKELVD
                     SYMRRHKVKPGITGLAQVRGYRGETDTVDKMKLRVESDLEYIENWSLGLDLKIILQTI
                     FKGFVNPNAY"
     gene            2502..3917
                     /locus_tag="PLQ18_04865"
     CDS             2502..3917
                     /locus_tag="PLQ18_04865"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis polyprenyl
                     glycosylphosphotransferase"
                     /protein_id="HNE01019.1"
                     /translation="MKRALVAESSVMRGLLARSLDIATIVASSFAGWWLETGSTGWFE
                     GDFLRAALIGLLLTGICSAHWAVYRSWRGTPLRSELEAVLGAWLLAVLLLVPVLELTG
                     EPDEYGRHWLLYWALFGTAGLAGWRVLMRRAQRYVHTGGRDVRRVVLVTNGGFGLQII
                     EQLATARWAGFEFVGWFDERDGDDGRISAAAVQRCLGPTERLAGFVEDGNADEVWIAL
                     PFRAEARLRSILWSLRHCTADIRLVPDISSFRLLNHSLGEVAGLPVINLSSTPMSGRN
                     RLLKAIEDRTLAGACLILAAPLLLVIALGVYLTTPGPLFFRPVRLGYDGRPFRAWKFR
                     CAVPHAGRNRFTRFGVFLRHTGLEGLPQLLNVLDGSMAIVGPCPHAAAYNAQYKARVD
                     GYMSRFKVKPGLTGWAQIHGHDQRIESIEDMERRLALDLEYIENWSLELDLRIVLLTV
                     WQMAGRVLGGLRKAAAQQIRH"
     gene            complement(3914..>4175)
                     /locus_tag="PLQ18_04870"
     CDS             complement(3914..>4175)
                     /locus_tag="PLQ18_04870"
                     /inference="COORDINATES: protein motif:HMM:NF018701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HNE01020.1"
                     /translation="EPLADALAAGRVGERPEGLIGAVTPDAGARALVERVNAERMARY
                     RDIAERNGRPLAEVQALAGRRLTAGAADGQYVMDAAGRWQRR"
BASE COUNT          606 a         1387 c         1467 g          715 t
ORIGIN      
        1 gtagccgacg aaggccgaag gatcggccag ggcggtcttc gtgccatcgg caagctgcgc
       61 gaccagcgtc gcgccgtcgc gcaggtattc ggtgacctcg gcgtgcttgc cgcttgcaag
      121 cggcacggcc tggtcgagga agccggcagc gtaggcgatg accttgtcgc cgcgcgccgg
      181 gttgtagccc ttgcccttct cggcgccgcc gtcctcgggc agcacgtcag tgccgtacag
      241 cgcgtcgtac agcgagcccc agcgggcgtt ggcggcattg agcgcgtagc gggcgttgtt
      301 gaccggcacc accagctgcg ggccggcaac cgaggcgatc tccgggtcga cattgtcggt
      361 cgagacctgg aagtcggggc cttcgggcag caggtagccg atctcgcgca ggaaggcgac
      421 ataggcggcg tgatcgtgcg gctggccgcg acgcgcgacg tgccaggcat cgatctgcgc
      481 ctgcagcgca tcgcgcttcg ccagcagttc gcggttgcgc ggggccagat ccgtgacgat
      541 ggcttccagc gccgtccacc agcctgcggc atcgaggccg gtaccgggca gcagcgtgtt
      601 ctggaccaga tcgtacaggg gcttggcaac ctgcaggccg ccgatggcaa cgcgctcagt
      661 catgtaggtt cgtctccgga ttgagggcag ggctgcggtc gtgcatacgg agggcacgac
      721 ggcgaggccg ggcattttcg ccggccgtaa cggcttgtgc aatgcgcaat gtccgctgcc
      781 gtccggcccc ggatgcagcg gtgcagtggt caggcagatt taaaaagcct gtcacaggca
      841 tccccggtaa tgcggggtgg ctctggtacg atgcgcgcct tcatgtgggg agcaagtgtc
      901 gtcggggcca acgacggctt tctgcagggg tgatgtgcga gcaggccagg gagcggcaag
      961 gcgggcccgc gcgcattgcc tcgcctcgcc ttattccgga tggtccggat tgcatgtggg
     1021 actcaggact cgtgaagggc attcttcaag aacattcggt ggttgtcgaa gcggtgctgc
     1081 gcacgctcga ctggcttgca attgccatgg ccggctggct ggccttcatg ctgcgcttcg
     1141 atgcggccgt gatgccgggt cccttccagc gggcgatgat catcgcactg ctggtcagca
     1201 tcggcgtctt cgggcgctgc agcgtctacc gggcctggcg tggcgaaacc ctgaacggcg
     1261 agctgcgcac gctgctgtgg gcctggtgcc tgaccttcct gatcacgatc ggtgcgctgg
     1321 ccctcaccac gggccgcgac gaagcgccgt ttccgcgcct ctggctgatg ctgtggttcg
     1381 tgctcggctg ctgcctgctg acactgctgc gcggctgcct gcgccaggtg ctgcgccggc
     1441 tgcgcagcag cggtcacaac acgcgccggg tggtcgtggt cgtggccggt gaactcggcg
     1501 tcagcgtcat ccgccggctg tgcgaggcgc gctggaccga actgcgcgtg gtcggctatt
     1561 tcgatgaccg gccgatcgag cgcgtcggcg agatcgccgg cgtgccgaga atcggacgca
     1621 tcgacgagct ggcggcctac gtcgaggggg gacacgccga tcaggtctgg atcacgctgc
     1681 cgttccgggc cgaggagcgc gtgcgcgaga tcctcgagac gctgcgtcac gcgaccgccg
     1741 acatccgcat ggtgcccgac atcctcggcc tgcagctgct gaaccattcg atgggcaccg
     1801 tcgccggcat cccggtcatc aacctgtcct cgacgccgat ggtcggcttc aatcgcgtca
     1861 tcaaggcgct ggaggatcgc atgatctcgc tggcgatcct gctgatgatc gcgccgctga
     1921 tgatcgcgat cgcgatcggc atcaagctgt catcgccggg gccggtgttc ttccgtcagg
     1981 agcggctcgg ctacgacggc cggccgttcc gggtctggaa gttccgcagc atgaaggtgc
     2041 atcaggagca cggcggccag gtcacgcagg cgacgcgcgg cgattcgcgg atcacgccgt
     2101 tcggtgcctt cctgcgccgc accagcctcg acgagctgcc gcagttcttc aacgtgctcg
     2161 acggcagcat gtcgatcgtc ggcccgcgcc cgcacgcggt cgcgcacaat caggaataca
     2221 aggaactcgt cgacagctac atgcgccggc acaaggtcaa gcccggcatc accgggctcg
     2281 cgcaggtccg tggctaccgc ggcgagaccg atacggtcga caagatgaag ctgcgcgtcg
     2341 agagcgatct cgaatacatc gagaactggt cactcggcct ggacctgaag atcatcctgc
     2401 agaccatctt caaggggttc gtgaatccga atgcctattg aaccgtccac cacctgcgct
     2461 gcggcatgga tcggcgaggc gatgggagta cggggcatcg catgaagcgc gccctcgtcg
     2521 ccgaatcttc ggtcatgcgc ggcctgctgg cgcgcagcct cgatatcgcc acgatcgtgg
     2581 cgagcagctt tgccggctgg tggctggaga ccggcagcac cggctggttc gaaggggatt
     2641 tcctgcgtgc cgccctgatc gggctgctgc tgaccggcat ctgctctgcg cactgggcgg
     2701 tgtaccgcag ctggcgcggc acgccgctgc gcagcgagct ggaggccgtg ctcggtgcct
     2761 ggctgctggc cgtgctgctg ctggtgccgg tgctcgaact gaccggcgag ccggacgaat
     2821 acggccggca ctggctgctg tactgggcgc tgttcggcac ggccgggctg gccggatggc
     2881 gggtgctgat gcgtcgcgcg cagcgctacg tccataccgg tggtcgcgac gtgcgccgcg
     2941 tggtgctggt caccaacggc ggcttcggat tgcagatcat cgagcagctc gccaccgcac
     3001 gctgggccgg cttcgaattc gtcggctggt tcgatgaacg cgacggcgat gacggccgca
     3061 tcagcgcagc ggccgtgcag cgctgcctgg gcccgaccga gcggctggcg ggcttcgtcg
     3121 aggacggcaa tgccgacgag gtctggatcg cgctgccctt ccgcgccgag gccaggctgc
     3181 gctcgatcct ctggagcctg cgccactgca cggccgacat ccggctggtg ccggacatct
     3241 ccagcttccg gctgctgaac cattcgctcg gcgaggttgc aggtctgccg gtcatcaacc
     3301 tgtcctcgac gccgatgagc ggccgcaacc gcctgctgaa ggccatcgag gaccgcacgc
     3361 tcgccggcgc ctgcctgatc ctcgctgcac cgctgctgct cgtgatcgca ctcggggtct
     3421 acctgaccac gccggggccg ctgttcttcc ggccggtacg cctcggctat gacggccggc
     3481 cgttccgcgc ctggaagttc cgctgcgcgg tgccgcacgc cggccgcaac cgcttcacgc
     3541 gcttcggcgt gttcctgcgc cacaccggtc tcgaaggcct gccgcagttg ctgaacgtgc
     3601 tcgacggcag catggccatc gtcggaccgt gtccgcacgc cgccgcctac aacgcccagt
     3661 acaaggcacg ggtcgacggc tacatgagtc gtttcaaggt caagccgggg ctgaccggct
     3721 gggcgcagat ccatggccac gaccagcgca tcgaatcgat cgaggacatg gagcggcgtc
     3781 tggcgctcga tctcgaatac atcgagaact ggtcgctgga actcgacctg cgcatcgtgc
     3841 tgctgacggt ctggcagatg gccgggcgcg tgctcggggg gctgcgcaag gccgccgccc
     3901 agcagatccg ccactaacgc cgctgccagc ggccggctgc gtccatcacg tactgtccgt
     3961 cagccgcgcc ggccgtcagc cggcggccgg cgagcgcctg cacttcggcg agcgggcggc
     4021 cgttgcgctc ggcgatgtcg cggtaacggg ccatgcgctc ggcgttgaca cgctcgacca
     4081 gcgcgcgcgc gccggcatcc ggcgtgaccg cgccgatcag cccttcgggc cgctcgccga
     4141 cccggccggc cgcgagggca tcggccagcg gttcg
//
LOCUS       DAOQJH010000158         4161 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_13019_length_4161_cov_3.419873, whole genome shotgun sequence.
ACCESSION   DAOQJH010000158 DAOQJH010000000
VERSION     DAOQJH010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 4161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4161
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13019_length_4161_cov_3.419873"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..653)
                     /gene="tsaB"
                     /locus_tag="PLQ18_04875"
     CDS             complement(<1..653)
                     /gene="tsaB"
                     /locus_tag="PLQ18_04875"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HNE01021.1"
                     /translation="MNLLAIDTATEACSAALLAGDELIERWQLAPRGHTQLLLPMIGA
                     VMAEAGCTWSALDGIAFGRGPGAFVGVRIATACAQGLAFAHDLPVAPVSTLETLAYGA
                     GRRQGARQVFAAIDARMGEIYAAAWPLDADGWPQEPRLAECVTAASALSLPGTWFGVG
                     SGLAAAAAAFDGRIAFSDRDPAALPHAADVARLGRRLLLAGAGVSAAAEAGVDAGGLR
                     "
     gene            complement(749..1273)
                     /locus_tag="PLQ18_04880"
     CDS             complement(749..1273)
                     /locus_tag="PLQ18_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002934334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HNE01022.1"
                     /translation="MQALKPSAGDSAARASRQYLTFTVAGEAYAFGIARIREIIEYGR
                     LTPIPLMPAFVRGVINLRGRVLPVIDLAARFGGPPGETTRRSCIVVVEADADADRGGT
                     DLGFVVDTVSEVLEIPLERIEPPPAFGQHIRPDFIEGMARLDERFVVILDEHRVLSIP
                     EMAQLSRLTQGAAA"
     gene            complement(1295..2944)
                     /locus_tag="PLQ18_04885"
     CDS             complement(1295..2944)
                     /locus_tag="PLQ18_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014086713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNE01023.1"
                     /translation="MKNLKVVTRLGLGFGLILLALAAQIIVGFNGLDRINDEVSDIVH
                     DRIPKMQWTGDLAQSVATISIAMRDAMLSGRAEDVRDAIAQVEESKRTIAERLEKLQS
                     SISNERGRVLLKGVDDARAAYLNDQADMLTQLRAGDVEQAKRVLSERLRRSQDDYLVA
                     VRKLDAFQAETMRMLGDQADAEVRGNQRMMLILGLCALAGGLLSCWWFTRSLMRQLGG
                     EPGYAAELLHGVAEGRLDMQVVLRNNDTSSMLYALKTTLERLSQLISEVRSAAGVLGS
                     ASEQINATAQTLSQSSAEQAASVEETSASIEQMSATIALNTDNARITDGVAGKVTREA
                     DEGGVAVRDTVAAMKQIAGRISIVDDIAYQTNLLALNAAIEAARAGDHGKGFAVVAAE
                     VRKLAERAQIAAQEISKLATSSVETAEHAGDLLAEIVPAVRKTSDLVQEIAAASTEQA
                     GGVAQINGAMGQLSQATQQNASASEELAATAEEMASQAEQLMQLMDAFRLAGDNAVVS
                     VQPRARASRPAGAALKRPQANAGLLRAFAAGEPEESGFVRY"
     gene            complement(3007..>4161)
                     /locus_tag="PLQ18_04890"
     CDS             complement(3007..>4161)
                     /locus_tag="PLQ18_04890"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HNE01024.1"
                     /translation="ELIERIGELVTVGAGATLLARELGLPALDELATTLNRLVSGIRD
                     NALQLRMVPIGETFARFPRVVRDLGQELGKDIDLELCGEDTELDKAIVEPLVDPLMHL
                     VRNAVDHGIEPLAVRRARGKPGVGRVRLSARHEGANMLIEVADDGGGLDGERIRSKAV
                     AAGLIGAGEHLDAQGLARLIFAPGLSTRDTVTDVSGRGVGLEVVQRNIEALRGSIEIE
                     SEAGAGTTVRLRLPLTLAIIDGFLIRCAGTSFVLPLDQVEECLDFDVAALHAPPRQLD
                     LRGRPLPLIRLHEFFGLGTPCGRRASVVVVRCGERRAGLLVDELLGEFQTVIKPLGPL
                     FEHLRALGGTTILGSGEVALIVDVPALFALAAARTPPASSLPAPAKLACA"
BASE COUNT          643 a         1439 c         1447 g          632 t
ORIGIN      
        1 cgcagccccc ccgcgtccac gcccgcctcc gcagccgccg acacgccggc accggccagg
       61 agcaggcgcc tgccgagccg ggcgacgtcg gcggcgtgcg gcagcgcggc cggatcacgg
      121 tcactgaagg cgatgcgccc gtcgaatgcg gcagccgccg ccgcgagacc gctgccgacc
      181 ccgaaccagg tgccgggcag gctcagcgcc gaggctgccg tgacgcattc ggcgagccgg
      241 ggctcctgcg gccagccatc ggcatcgagc ggccaggctg cggcgtagat ctctcccatg
      301 cgcgcatcga tcgccgcgaa cacctgacgc gcgccctgcc tgcgaccggc gccgtacgcg
      361 agggtttcga gcgtcgagac cggcgccacc ggcaaatcgt gcgcgaaggc cagcccctgt
      421 gcgcaggcgg tggcgatgcg cacgccgacg aaggcgcccg gcccgcgccc gaaggcgatg
      481 ccatcgagcg ccgaccatgt gcagccggcc tcggccatca ccgctccgat catcggcagc
      541 agcagctgcg tgtggccgcg cggcgcgagc tgccagcgct cgatcagctc gtcaccggcg
      601 agcagggcgg ccgagcaggc ttcggtggcg gtatcgatgg cgaggaggtt caagcggcgg
      661 gctccggtgg cggacaggcg cgcggcagta tgccatcggc cccggaaacc ggatgtcgtc
      721 ggcccggcgg acgcccgcgc tgccgcactc aggccgccgc cccctgcgtc agccgtgaca
      781 gctgcgccat ctcggggatt gacagcacgc ggtgctcgtc gaggatcacg acgaagcgct
      841 cgtcgagccg cgccatgcct tcaatgaagt ccgggcggat gtgctgaccg aaggccggag
      901 gcggctcgat gcgctcgagc gggatttcca gcacctcgga gacggtatcg acgacgaagc
      961 cgagatcggt gccgcctctg tcggcatcgg catccgcttc gacgacgacg atgcagcttc
     1021 tgcgcgtcgt ctcacccggc gggccgccga agcgcgcggc caggtcgatg accggcagca
     1081 cgcggccgcg caggttgatg acgccgcgca cgaaagccgg catcagcggg atcggcgtca
     1141 gccgtccgta ttcgatgatc tcgcggatgc gggcgatgcc gaaggcatag gcttcgccgg
     1201 cgaccgtgaa ggtcaggtac tgacggctgg cacgcgcggc gctgtcgccg gcggaaggtt
     1261 tcagggcctg catggtggaa gtcctgccgg tggctcagta acggacaaaa ccggattcct
     1321 cgggctcgcc ggcggcgaag gcacgcagca gtccggcgtt cgcctgcggc cgcttcagcg
     1381 cagcaccagc cggccgcgac gcacgggcac gcggctggac gctgaccacg gcgttgtcgc
     1441 cggcaagacg gaaggcatcc atcaactgca tcagctgttc ggcctgactc gccatctctt
     1501 cggcagtcgc cgccagttcc tcggaggccg aggcgttctg ctgcgtggcc tggctgagct
     1561 ggcccatcgc gccgttgatc tgcgccacgc cgccggcctg ctcggtcgag gcggcggcga
     1621 tctcctgcac cagatcggag gtcttgcgca ccgccggcac gatctcggcg agcaggtcgc
     1681 cggcgtgctc ggcggtctcg acgctgctgg tggcgagttt gctgatttcc tgtgcggcga
     1741 tctgggcgcg ctcggcgagc ttgcgcacct cggcagccac gacggcaaag cccttgccgt
     1801 ggtccccggc gcgggcggcc tcgatcgcgg cattcagcgc cagcaggttg gtctggtagg
     1861 cgatgtcgtc gacgatgctg atgcgcccgg cgatctgctt catcgctgcc accgtgtcgc
     1921 gtaccgccac accaccttcg tcggcctcgc gcgtgacctt gccggccacg ccgtcggtga
     1981 tgcgggcgtt gtcggtattc agcgcgatcg tggccgacat ctgctcgatc gaggcactgg
     2041 tctcctcgac gctcgcggcc tgttcagcgc tggattgcga cagggtctgt gcagtggcgt
     2101 tgatctgctc ggacgccgag ccaagcacac cggcggccga acgcacttcg gagatcagct
     2161 gcgacaggcg ttcgagggtg gtcttcagtg cgtacagcat gctgctggtg tcgttgttgc
     2221 gcagcaccac ctgcatgtcg agccggcctt cggcgacccc gtgcagcagc tcggccgcgt
     2281 agcccggttc accaccgagc tggcgcatca gggaacgcgt gaaccaccag caggacaaca
     2341 gccccccggc cagcgcgcag agcccgagga tcagcatcat gcgctggttg ccacggactt
     2401 cggcatcggc ctgatctccg agcatgcgca tggtttcggc ctgaaaggca tcgagcttgc
     2461 gcacggcgac cagataatca tcctgcgaac gacgcaagcg ctcgctcagc acacgcttgg
     2521 cctgctcgac atccccggca cgcagctgcg tcagcatgtc ggcctgatcg ttcagatagg
     2581 ccgcgcgcgc atcgtcgacc cccttgagca gcacacgacc gcgttcgttg ctgatgctcg
     2641 actgcagctt ttcgagccgc tcggcaatcg tgcgcttgga ttcctcgacc tgggcgatgg
     2701 catcgcgaac atcctcggca cggcccgaga gcatggcatc gcgcatcgcg atcgagatgg
     2761 tggccacgct ctgggcgaga tcgccggtcc actgcatctt cggaatgcgg tcgtggacga
     2821 tgtccgagac ttcgtcattg atgcggtcca gaccgttgaa gccaacgatg atctgggccg
     2881 cgagtgcgag caggatcagg ccgaagccca gccccaggcg ggtgactact ttcaggtttt
     2941 tcatacagga gccccttgga tgcggatgat cgggaaatga tggattcggg gagactgaga
     3001 cgggattcag gcgcaggcca gcttcgccgg cgccgggaga ctcgatgcag gcggcgtgcg
     3061 cgccgctgcc agcgcgaaca gcgccggcac atcgacgatc agcgcgactt cgccgctgcc
     3121 gaggatcgtc gtgccgccga gtgcgcgcag gtgctcgaac agcggtccga gcggcttgat
     3181 gacggtctgg aattcgccga gcagttcatc gaccagcagg ccggcgcggc gctcgccgca
     3241 gcgcacgacc acgacgctgg cgcgccggcc gcacggcgtg ccgaggccga agaattcatg
     3301 caggcggatc agcggcagcg gcctgccgcg caggtcgagc tggcggggcg gggcatgcag
     3361 cgccgcaacg tcgaaatcca ggcattcctc gacctggtcg agcggcagca cgaagctcgt
     3421 gccggcgcag cggatcagga agccgtcgat gatcgccagc gtcagcggca ggcgcaggcg
     3481 cacggtcgtg ccggccccgg cttcggattc gatttcgatg ctgccgcgca gcgcctcgat
     3541 gttgcgctgc acgacctcca ggccgacacc gcggcccgag acatccgtga ccgtgtcgcg
     3601 cgtcgacagt cccggcgcga agatcagcct tgccagtccc tgtgcatcga ggtgctcgcc
     3661 ggcaccgatc aggccggcgg cgacggcctt gctgcggatg cgctcgccat ccaggccgcc
     3721 accgtcatcg gcgacctcga tcagcatgtt cgcgccctcg tggcgggccg acagccgcac
     3781 gcgaccgacg ccgggcttgc cgcgcgcacg gcgcaccgca agcggctcga tgccgtgatc
     3841 gacggcgttg cgcaccaggt gcatcagcgg atcgaccagc ggctcgacga tggccttgtc
     3901 gagttcggtg tcctcgccgc agagctcaag atcgatgtcc ttgccgagtt cctggccgag
     3961 gtcgcgcacg acgcgtggaa agcgcgcgaa ggtctcgccg atcggcacca tgcgcagctg
     4021 cagcgcgttg tcgcggatgc cggaaaccag ccggttcagc gtcgtcgcca gctcatcgag
     4081 cgccggcagg ccgagttcgc gcgcgagcag cgtcgcgccg gccccgacgg tcacgagttc
     4141 gccgatgcgc tcgatcagct c
//
LOCUS       DAOQJH010000159         4131 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_13160_length_4131_cov_4.790236, whole genome shotgun sequence.
ACCESSION   DAOQJH010000159 DAOQJH010000000
VERSION     DAOQJH010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 4131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4131
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13160_length_4131_cov_4.790236"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            396..1787
                     /gene="sthA"
                     /locus_tag="PLQ18_04895"
     CDS             396..1787
                     /gene="sthA"
                     /locus_tag="PLQ18_04895"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016353577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (B-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HNE01025.1"
                     /translation="MSEQKYDVIVIGTGPGGEGAAMKATKSGKLVAVVEKYNAVGGGC
                     THWGTIPSKALRHAINRMMEFNRSALYRSLGRAVRFSYPDLLKTAESVIAQQATVRRD
                     FYDRNGIPIYRGTARFVDAHTIELRGAQPVRLRADHFVLATGSRPYRPSDVDFNHPRV
                     FDSDTILSMAYTPESISIYGAGVIGCEYASMFRNMGCKVNLINTRDQLLSFLDDEITH
                     ALNYHLRDQGVVIRHNETYERVEAQSDGVVMHMKSGKRIKSDIVLWANGRTGNAQHMG
                     LEELGIRPNGRGQIDVNAHYQTALDHIYAVGDVIGYPSLASASYDQGRFAATHMADGV
                     CDSRLVEYVPTGIYTSPEISSVGRTERELTEACIPYEVGYAWFKSLARAQITGKTVGM
                     LKLLFHRDTLELLGIHCFGDQAAEIVHIGQAIMAQRGEANSLLYFVNTTFNYPTMAEA
                     YRVAALNGLNRAR"
     gene            1887..3017
                     /locus_tag="PLQ18_04900"
     CDS             1887..3017
                     /locus_tag="PLQ18_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017463508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HNE01026.1"
                     /translation="MDVTPYLKLMVDREASDLFFYVGAPVNIKIEGTVMPLGNVPLKP
                     GAVREIAYSLMTDSQIREFERDLESNFALSVSEIGRFRANVFRQRGEVSMVIRYIKQR
                     IPDFVELSLPQILGNLVMEKRGLVLMVGSTGSGKSTSLAAMIDHRNSNAAGHILTIED
                     PIEFVHHHKKSVIGQREIGIDTHSYGDALKNALREAPDVILIGEVRDAETMKYSISYA
                     ETGHLCLSTLHANHANGALERIINFFPEIARPQLLMDLSQNLRAIVSQRLIIGVDGRR
                     VPAVEIMLNTPYISELILKGKFESIKEAMAQGTEIGMKTFDQALYDLYKGGRISKDEA
                     VRNADSRNNVSLQIRLSEGMGQGKAAPDPLGASLKVTPKDVR"
     gene            3123..>4131
                     /locus_tag="PLQ18_04905"
     CDS             3123..>4131
                     /locus_tag="PLQ18_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008931708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNE01027.1"
                     /translation="MTRPRIAARMAGIAPFHVMELLARAQALEAAGRDIVHMEVGEPD
                     FVTAAPVVEAGRRALAEGRTAYTAAKGLTALRTAISDDYARRYGVGVDPDCILVTPGA
                     SGALLLAAGVLLDPGERCLLADPSYPCNRHFVRFCGAEALGIPVGADTRFQLTPELVA
                     AHWDARTAAVMLASPSNPTGTCVPPAALAGIHAEVRARGGALIVDEIYQGLVYDGEPA
                     TALAGGEDVFVINSFSKYFGMTGWRLGWLVAPASHVREAEKLAQNLFLAASTPAQHAA
                     LAAFTPEAEAIFVERREAFRRRRDRLVPALRELGFGVPVQPDGAFYVYADAGRHTDDA
                     YA"
BASE COUNT          711 a         1381 c         1390 g          649 t
ORIGIN      
        1 ctcgtgcctc cgagcgccag agcggcgccg agcgcgaccg caatctgctt cttcatcatg
       61 tgtttagccc tttggttcag atcgtaaggc cggaagccag gagcgcggcg ccagtccgcg
      121 ggaattagcc tcagtcctga gagcagcgcg ctgtctcctg tcaggcaagc aatgggccat
      181 gattgataac atgatgaata taaagggatg tggctagggt gatatgggag aggatgaacc
      241 cacatgtaaa ataatctgac tgcagtggag gccgccggcc gccggccggg ggggcgggcg
      301 gcttgtcggc gtgttgccgg tatactgcgg cgcaccggcc ggtcatggcg tgcatccggc
      361 ggcatgccgc aggtcaacat cagcaagggg cagagatgag cgagcagaag tacgacgtga
      421 tcgtgatcgg cacgggaccg ggcggcgaag gggcggcgat gaaggccacc aagtcgggca
      481 agctggttgc agtggtcgag aagtacaacg ccgtcggtgg cggctgcacg cactggggca
      541 cgatcccgtc caaggccctg cgccatgcga tcaaccggat gatggagttc aaccgcagcg
      601 cgctgtaccg cagcctcggc cgggcggtgc ggttttcgta ccccgacctg ctgaagacgg
      661 ccgagtcggt gatcgcgcag caggcgaccg tgcgccgcga cttctacgac cgcaacggca
      721 tcccgatcta tcgcggcacc gcccgcttcg tcgatgcgca cacgatcgag ctgcggggcg
      781 cccagccggt gcggctgcgc gcggatcact tcgtcctcgc caccggttca cgtccgtacc
      841 ggccgtccga cgtcgacttc aaccatccgc gcgtgttcga cagcgacacg atcctgagca
      901 tggcctacac gccggagtcg atctcgatct acggcgcggg cgtgatcggc tgcgaatacg
      961 cgagcatgtt ccgcaacatg ggctgcaagg tgaacctgat caacacccgc gaccagttgc
     1021 tgtccttcct cgatgacgag atcacgcacg cgctgaacta tcacctgcgc gaccaggggg
     1081 tcgtgatccg ccacaacgag acctacgagc gcgtcgaggc gcagtccgac ggcgtggtca
     1141 tgcacatgaa gtccggcaag cgcatcaaga gcgacatcgt gctgtgggcc aacggccgca
     1201 ccggcaacgc ccagcacatg gggctcgaag aactcggcat ccgcccgaac ggccgcggcc
     1261 agatcgatgt caacgcgcac taccagacgg cgctcgatca catctacgca gtcggggatg
     1321 tcatcggcta tccgagtctc gccagcgcct cctacgatca ggggcgcttc gccgcgacgc
     1381 acatggccga cggcgtctgc gacagccggc tcgtcgagta cgtgccgacc ggcatctaca
     1441 cctcgccgga gatcagctcg gtcgggcgca ccgagcggga gctgaccgag gcctgcatcc
     1501 cgtacgaggt cggctatgcg tggttcaaga gcctcgcgcg tgcgcagatc accggcaaga
     1561 ccgtcggcat gctgaagctg ctgttccacc gcgatacgct ggaactgctc ggcatccact
     1621 gcttcggcga tcaggccgcc gagatcgtgc acatcggcca ggcgatcatg gcgcagcgcg
     1681 gcgaggccaa ttcgctgctg tacttcgtca acacgacctt caattatccg accatggccg
     1741 aggcgtaccg tgtcgcggcc ctgaacggac tgaaccgcgc ccgctgatcc gcagcgggcg
     1801 aggcaggtcg tggcggcagg cagcggcttc gcgcaggcgc ctggtgcgcc gcggcggcgg
     1861 cagtgatcga tggggggagg cagtccatgg atgtcacacc gtatctgaag ctgatggtgg
     1921 accgcgaggc ctcggatctc ttcttctacg tgggggcgcc ggtcaacatc aagatcgaag
     1981 gcacggtcat gccgctcggc aacgtgccgc tgaagcccgg tgcggtgcgc gagattgcct
     2041 actcgctgat gaccgacagt cagatccgcg aattcgaacg cgacctcgaa tcgaacttcg
     2101 cgctgtcggt cagcgagatc ggccgtttcc gcgccaacgt cttccgccag cgcggcgagg
     2161 tgtcgatggt catccgctac atcaagcagc ggattccgga tttcgtcgag ctgagcctgc
     2221 cgcagatcct cggcaatctg gtcatggaga agcgcgggct ggtgctgatg gtcggctcga
     2281 ccggctcggg caagtcgacc tcgctcgcgg cgatgatcga tcaccgcaac agcaatgccg
     2341 ccggccacat cctgaccatt gaagacccga tcgaattcgt gcatcaccac aagaagtcgg
     2401 tgatcggtca gcgcgagatc ggcatcgaca cgcacagcta cggcgatgcg ctgaagaacg
     2461 cgctgcgcga ggcgccggac gtgatcctga tcggcgaggt gcgcgacgcc gagacgatga
     2521 agtactcgat cagctacgcc gagaccggcc acctgtgcct gtcgacgctg cacgccaacc
     2581 atgccaacgg cgcgctggag cgcatcatca acttcttccc ggaaatcgcg cgaccgcagc
     2641 tgctgatgga cctgtcgcag aacctgcgcg cgatcgtgtc gcagcgcctg atcatcgggg
     2701 tcgacggccg gcgcgtgccg gcggtcgaga tcatgctgaa cacgccgtac atctccgagc
     2761 tgatcctcaa gggcaagttc gagagcatca aggaggcgat ggcgcagggc accgagatcg
     2821 gcatgaagac cttcgaccag gcgctgtacg acctgtacaa gggcgggcgc atcagcaagg
     2881 acgaggccgt gcgcaacgcc gattcgcgca acaacgtcag cctgcagatc cgcctgtccg
     2941 aaggcatggg tcagggcaag gcagcgccgg acccgctcgg tgcctcgctg aaggtcactc
     3001 cgaaggacgt gcgctgagcg cgcacccgcc gttttcccgc ccgcgcgacc gcagcccgct
     3061 gcggtcgcgt cgttttggcg gacctgaagg ttcatgccga tgactgtcga ggaagcctcc
     3121 cgatgacccg tccccgcatc gccgcacgga tggccggcat cgccccgttt cacgtcatgg
     3181 aactgctcgc ccgcgcccag gcgctcgaag ccgccggccg cgacatcgtg cacatggagg
     3241 tcggcgagcc ggatttcgtc acggccgcgc cggtcgtcga ggccggccgg cgtgcgctcg
     3301 ccgaggggcg caccgcgtac acggccgcca aggggctcac ggcgctgcgc acggcgatca
     3361 gcgatgacta cgcacgccgc tacggcgtcg gcgtcgatcc ggactgcatc ctcgtcacgc
     3421 ccggtgccag cggtgcgctg ctgctcgccg ccggcgtgct gctcgatccg ggcgagcgct
     3481 gcctgctcgc cgatccgagc tatccgtgca accggcattt cgtgcgcttc tgcggcgccg
     3541 aggcgctcgg catcccggtc ggggccgaca ctcgcttcca gctgacgccg gagctcgtcg
     3601 ccgcgcactg ggatgcgcgc accgcggcgg tgatgctcgc ctcgccgtcg aatccgaccg
     3661 gcacctgcgt gccgccggcg gccctggccg gcatccacgc cgaggtgcgc gcgcgcggcg
     3721 gggcgctgat cgtcgacgag atctatcagg gtctggtcta cgacggggag ccggcgacgg
     3781 cgctcgccgg gggcgaggac gtgttcgtga tcaacagctt ctcgaagtac ttcggcatga
     3841 ccggctggcg gctcggctgg ctggtggcgc cggcctcgca cgtgcgcgag gccgagaagc
     3901 tcgcgcagaa cctgttcctt gccgcctcga cgccggccca gcacgccgca ctcgcggcct
     3961 tcacgcccga ggccgaggcg atcttcgtcg agcggcgcga ggccttccgc cgccggcgcg
     4021 accggctggt gccggcgctg cgcgaactcg gcttcggcgt gccggtgcag ccggacggcg
     4081 cgttctacgt ctacgccgac gccggccggc acacggacga tgcctacgcg t
//
LOCUS       DAOQJH010000160         4123 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_13205_length_4123_cov_4.222714, whole genome shotgun sequence.
ACCESSION   DAOQJH010000160 DAOQJH010000000
VERSION     DAOQJH010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 4123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4123
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13205_length_4123_cov_4.222714"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1588)
                     /locus_tag="PLQ18_04910"
     CDS             complement(<1..1588)
                     /locus_tag="PLQ18_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003497960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNE01028.1"
                     /translation="MPAEAPEVRTTCPYCGVGCGVIAYRTDDGTIAVRGDEQHPANFG
                     RLCSKGSALAETLALEDRLLHPEIDGQRVGWDAALDAVAEGFRRTIAEHGPDAVALYV
                     SGQLLTEDYYVANKLMKGCIGTSNIDTNSRLCMSSSVAGHKRAFGTDTVPGTYEDLEQ
                     ADLLILVGSNLAWCHPVLYQRILAARQARGGKPKLIVIDPRRTASCDQAEQHLAIAPN
                     TDTVLFNGLLVHLHRQDAVDLDYLDAHTDGYLAALRAAREIAPSIPEVARRCGLDEEA
                     VANFYGDFTRTKKVVTVYSQGVNQSAHGTDKVNAIINCHLATGRIGKPGCGPFSVTGQ
                     PNAMGGREVGGLANQLAAHMDFAPADVERVARFWQAPNIARGPGLKAVELFRAVAEGR
                     IKAIWIMGTNPVVSLPEADAVREALMNCPLVVVSDAVRVTDTTACADILLPAATWGEK
                     DGTVTNSERRISRQRAFLDLPGEARPDWWALAEVGRRLGFTASLDFAGPAAVFREFAA
                     LSGYENAAWGGDGVDIGWTPF"
     gene            complement(1737..2090)
                     /gene="nirD"
                     /locus_tag="PLQ18_04915"
     CDS             complement(1737..2090)
                     /gene="nirD"
                     /locus_tag="PLQ18_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009762758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008942 - nitrite reductase [NAD(P)H]
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HNE01029.1"
                     /translation="MSDWHDIGTVEDIPRLGARVVQSPQGNIAVFRTAEDEIFALRDR
                     CPHKAGPLSQGIVHGKRVTCPLHSWTLELGTGEAIAPDVGCAARFPVKVEGGRILLQV
                     SAVAAVSDHSAGTLA"
     gene            complement(2215..>4123)
                     /gene="nirB"
                     /locus_tag="PLQ18_04920"
     CDS             complement(2215..>4123)
                     /gene="nirB"
                     /locus_tag="PLQ18_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018876962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008942 - nitrite reductase [NAD(P)H]
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0042128 - nitrate assimilation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nitrite reductase large subunit NirB"
                     /protein_id="HNE01030.1"
                     /translation="LDTLMERQLDPFAADMLRKTLEERGLRFLMGAHTAAILGDDKVR
                     GVRFKDGLEVPADIVVMAVGIRPNIELAKKSGIHCERGIVVNDTMQTYDPRVYSVGEC
                     VQHRGVCYGLVAPLWEQAKVCASHLAQHGVGRYEGSVTSTKLKVTGIDLFSAGNFNGG
                     PDCEELVLRDPARGVYKKLVVKDNRVQGAVLYGDTVDGTWYFQLLRENTDITAFRETV
                     LFGHAHMADAGREGHSVMSMSDDAEICGCNGVCKGTIVKAITEKKLFTVDDVRAHTKA
                     SASCGSCTGLVEQILAATVGNYDATPKEKPMCKCTDHPHDVVRKAIRDEHLTSLESVF
                     KVLNWKTANGCASCRPALNYYLISTWPAEAKDDYQSRFINERVHANIQKDGTYSVVPR
                     MWGGLTNARELRAIADAADKYNVRTVKVTGGQRIDLFGIRKEDLPKIWADLNAAGMVS
                     GHAYGKSLRTVKTCVGSDWCRFGTQDSTGLGVKIEQMTWGSWTPHKFKMAVSGCPRNC
                     AEATIKDFGVVCVDSGYEAHVGGNGGIHVRGTDLLCKLETEEQVLEYCGAFMQLYREE
                     GRYLERTAPWIERVGLEYVKAKIVEDEEGRKALYARFLESQKYAQTDPWAERASAGVD
                     AHEYAPVATIG"
BASE COUNT          618 a         1364 c         1440 g          701 t
ORIGIN      
        1 ggaagggcgt ccagccgatg tcgacgccat cgccgcccca ggcggcgttc tcgtagccgg
       61 acagcgccgc gaactcgcgg aacaccgcgg ccggaccggc gaaatcgaga ctcgccgtga
      121 atccaagccg gcggccgact tcggcaagcg cccaccagtc ggggcgcgct tcaccgggca
      181 gatccaggaa ggcgcgctgg cgcgagatgc ggcgttcgga attggtgacc gtgccgtcct
      241 tctcgcccca ggttgcggcc ggcagcagga tgtcggcgca ggccgtggtg tcggtgacgc
      301 gcacggcatc ggagacgacg accagcgggc agttcatcag cgcctcgcgc actgcatcgg
      361 cctccggcag gctgaccacc ggattcgtgc ccatgatcca gatcgccttg atcctgccct
      421 cggcgacggc gcggaacagc tcgaccgctt tcaggcccgg gccgcgcgcg atgttcggcg
      481 cctgccagaa gcgggcgacg cgctcgacat cggccggagc gaagtccatg tgcgcggcga
      541 gctggttggc gaggccgccg acctcgcgtc cgcccatcgc gttcggctgg ccggtgaccg
      601 agaacggacc gcagccgggc ttgccgatgc gtccggtcgc cagatggcag ttgatgatgg
      661 cgttgacctt gtcggtgccg tgcgccgact ggttgacgcc ctgcgaatag acggtgacga
      721 ccttcttcgt gcgcgtgaaa tcgccgtaga agttcgcgac cgcctcctcg tcgagaccgc
      781 agcggcgcgc gacctcgggg atcgacgggg caatctcgcg cgctgcgcgc agcgccgcca
      841 gataaccgtc ggtgtgcgca tcgaggtagt cgagatcgac ggcatcctgc cggtgcaggt
      901 gcacgagcag gccgttgaac agcacggtgt cggtgttcgg cgcgatcgcc agatgctgct
      961 cggcctgatc gcagctcgcg gtgcggcgcg ggtcgatgac gatcagcttc ggcttgccgc
     1021 cgcgcgcctg gcgggcggcg aggatgcgct ggtacagcac cgggtggcac caggcgaggt
     1081 tcgagccgac gaggatcagc aggtcggcct gttcgagatc ctcgtaggtg ccgggcacgg
     1141 tgtcggtgcc gaacgcgcgc ttgtggccgg cgaccgacga ggacatgcac agccgcgaat
     1201 tggtgtcgat gttcgaggtg ccgatgcagc ccttcatcag cttgttggcg acgtagtagt
     1261 cctcggtcag cagctggccg gagacgtaca gcgccacggc atccgggccg tgctcggcga
     1321 tcgtgcgccg gaagccctcg gcgaccgcat cgagcgccgc atcccatccg acccgctggc
     1381 cgtcgatttc cggatgcagc agacggtctt cgagcgcgag cgtttcggcc agcgccgaac
     1441 ccttcgagca aagccgtccg aagttggccg gatgctgctc gtcgccgcgc acggcgatcg
     1501 tgccgtcatc cgtgcggtag gcgatgacgc cgcagccgac accgcaataa ggacaggtcg
     1561 tgcggacttc gggggcctcg gcaggcatgc gggaatctcc tggaacgatc gccgtgacgg
     1621 gaacgggcgc cggtcacgca cgccagcgcg tgcgcaaccc ttgatgcgtg ccgggacgcg
     1681 cggccccggc ggtgaggatc agcgcgggac ggcggacaac gccgccccgc gggctttcag
     1741 gccagcgtgc ccgcgctgtg atcggacacg gcggcgacgg cgctgacctg cagcaggatg
     1801 cgaccgccct ccaccttcac cgggaagcgg gccgcgcagc cgacgtcggg cgcgatcgcc
     1861 tcgccggtgc cgagctccag cgtccagctg tgcagcgggc aggtcacgcg cttgccgtgc
     1921 acgatgccct gcgacagcgg accggccttg tgcgggcagc ggtcgcgcag cgcgaagatt
     1981 tcatcctcgg ccgtgcggaa cacggcgatg ttgccctgcg ggctctgcac cacgcgcgca
     2041 ccgaggcgcg gaatgtcttc gacggtaccg atgtcgtgcc agtcgctcat ggggactctg
     2101 ctccggaccg tggtcatccc gggtgctgat gtggatccat gcacccggag tgacccggct
     2161 ttcagtattt atgatggtat aaaaatttat cttacgaagc ggatcggccg acgatcagcc
     2221 gatcgtcgcg accggcgcgt actcgtgcgc atcgacgccg gcgctggcgc gctccgccca
     2281 cggatcggtc tgcgcgtact tctgggattc caggaagcgc gcgtacagcg ccttgcggcc
     2341 ttcctcgtcc tcgacgatct tggccttcac gtattcgagg ccgacgcgct cgatccacgg
     2401 cgccgtgcgc tccagatagc ggccttcctc gcggtacagc tgcatgaagg cgccgcagta
     2461 ttcgagcacc tgctcctcgg tttcgagctt gcagagcaga tcggtgccgc gcacgtggat
     2521 gccgccgttg ccgccgacgt gggcctcgta gccggagtcg acgcagacca cgccgaagtc
     2581 cttgatcgtc gcctcggcgc agttgcgcgg gcagccggag acggccatct tgaacttgtg
     2641 cggcgtccac gagccccagg tcatctgctc gatcttgacg ccgagcccgg tcgaatcctg
     2701 cgtgccgaag cggcaccagt cggaaccgac gcaggtcttc acggtgcgca ggctcttgcc
     2761 gtaggcgtga cccgagacca tgccggcggc gttgagatcg gcccagatct tcggcaggtc
     2821 ttccttgcgg atgccgaaca ggtcgatgcg ctggccaccg gtgaccttga ccgtgcgcac
     2881 gttgtacttg tcggcggcat cggcaatcgc gcgcagttcc ctggcgttgg tcaggccgcc
     2941 ccacatgcgc ggcacgaccg agtaggtgcc gtccttctgg atgttggcgt gcacgcgctc
     3001 gttgatgaag cgcgactggt agtcgtcctt cgcctcggcc ggccaggtcg atatcaggta
     3061 gtagttcagc gccgggcggc aggacgcgca gccgttggcg gtcttccagt tcagcacctt
     3121 gaacacggat tcgagcgagg tcaggtgctc gtcgcggatc gccttgcgca cgacgtcgtg
     3181 cgggtgatcc gtgcacttgc acatcggctt ttccttcggc gtcgcgtcgt agttgccgac
     3241 ggtggccgcg aggatctgct cgaccagacc ggtgcacgaa ccgcacgagg ccgaggcctt
     3301 ggtgtgggcg cgcacgtcat cgaccgtgaa cagcttcttc tcggtgatcg ccttgacgat
     3361 cgtgcccttg cagacgccgt tgcagccgca gatctcggcg tcgtcggaca tcgacatgac
     3421 gctgtggcct tcgcggccgg catcggccat gtgcgcgtga ccgaacagca cggtctcgcg
     3481 gaaggcggtg atgtcggtgt tctcgcgcag cagctggaag taccaggtgc cgtcgacggt
     3541 atcgccgtag agcacggcgc cctgcacgcg gttgtccttg acgaccagct tcttgtagac
     3601 gccgcgggcc gggtcgcgca ggacgagttc ctcgcagtcc gggccaccgt tgaagttgcc
     3661 ggccgagaac aggtcgatgc cggtgacctt gagcttggtg ctcgtcaccg agccctcgta
     3721 gcggccgacg ccgtgctgcg cgaggtggct ggcacagacc ttggcctgct cccacagcgg
     3781 cgcgaccagg ccgtagcaga cgccgcggtg ctgcacgcat tcgccgaccg agtacacgcg
     3841 cgggtcgtag gtctgcatcg tgtcgttgac gacgatgccg cgctcgcagt ggatgccgga
     3901 cttcttcgcg agctcgatgt tcgggcggat gccgacggcc atgacgacga tgtcggccgg
     3961 cacttcgagg ccgtccttga agcgcacgcc gcggaccttg tcgtcaccga ggatcgcggc
     4021 ggtgtgcgcg cccatcagga agcgcaggcc gcgctcctcc agggtcttgc gcagcatgtc
     4081 ggccgcgaac gggtcgagct ggcgctccat cagcgtgtcg agc
//
LOCUS       DAOQJH010000161         4123 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_13206_length_4123_cov_3.978368, whole genome shotgun sequence.
ACCESSION   DAOQJH010000161 DAOQJH010000000
VERSION     DAOQJH010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 4123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4123
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13206_length_4123_cov_3.978368"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /locus_tag="PLQ18_04925"
     CDS             complement(<1..245)
                     /locus_tag="PLQ18_04925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HNE01031.1"
                     /translation="MPRRLIPGLLAAALAWPLTALAALPVPGFTARYSLALSGVPVGE
                     ATVTLDEPAPGRYRMRSEAAAKGQLAMLLPDRLSEEA"
     gene            complement(347..1006)
                     /gene="purN"
                     /locus_tag="PLQ18_04930"
     CDS             complement(347..1006)
                     /gene="purN"
                     /locus_tag="PLQ18_04930"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020210551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HNE01032.1"
                     /translation="MGSATKPGIVVLISGRGSNLQSILDRAASGELPVEVRAVISNRP
                     GVYGLQRARDAGVPALVLDHKDFADRTSFEAELIRQIDGFGPALVVLAGFMRILEPGF
                     CEHYRGRMLNIHPSLLPKYRGVHTHERALAAGETEHGVSIHFVTPELDGGPVFLQATV
                     PVLPGDDADTLAARVLVEEHKLYPLAIRWFAEGRVRLDGDTVLFDGAPLTAPKRLDDG
                     A"
     gene            complement(1006..2064)
                     /gene="purM"
                     /locus_tag="PLQ18_04935"
     CDS             complement(1006..2064)
                     /gene="purM"
                     /locus_tag="PLQ18_04935"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012970842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HNE01033.1"
                     /translation="MPVSDQTPPIRLSYRDAGVDIDAGEALVERIKPHARRTARPGVL
                     GGLGGFGALFELPLDRYRQPVLVSGTDGVGTKLRLALDTGLHDTIGIDLVAMCANDIA
                     VAGAEPLFFLDYYATGKLDVDTAATVIQGIADGCALAGCALVGGETAEMPGMYEGADY
                     DLAGFCVGIVEKERIIDGSRVAPGDVLIGLASSGPHSNGYSLIRRILAVTGTSLDEPF
                     AGTTLGRALLAPTRIYVPAMLKLLEQIDVHSFAHITGGGLPDNLPRVLADGTRAVIDA
                     ASWQRPEIFRWLQQQGGVIESEMYRTFNCGVGMVVCVAPADVETTLASLAASGETAWV
                     MGRIEAAGDDAQPRVEIR"
     gene            2274..2837
                     /locus_tag="PLQ18_04940"
     CDS             2274..2837
                     /locus_tag="PLQ18_04940"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005223839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HNE01034.1"
                     /translation="MRARDIPNLITGLRLLLVPPILWLLWHDDHGAALLLFLIAGASD
                     ALDGALARGFHWQSRLGGILDPIADKSLLVGCFLVLGAGGELPLWLVALVLGRDLVIV
                     LGAAAWHWLIGALEARPSLISKLNTCMQLAVVLAVLLSHGATPLPDWLLRLLEWTMAL
                     TTLWSGIDYVWHWSRLARRHVRGEVAG"
     gene            2834..3886
                     /locus_tag="PLQ18_04945"
                     /pseudo
     CDS             2834..3886
                     /locus_tag="PLQ18_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013983451.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
BASE COUNT          546 a         1448 c         1478 g          651 t
ORIGIN      
        1 tcggcttcct cgctcagccg atcgggcagc agcatcgcca gctggccttt tgcggctgct
       61 tcggagcgca tgcggtagcg ccccggtgcc ggctcatcga gcgtgacggt ggcctcgccg
      121 accggcacgc cggacagcgc cagcgaatag cgggccgtga agccgggcac cggcagcgcg
      181 gccagggcgg tcagcggcca ggccagcgcg gcggcgagca ggccagggat caggcggcgc
      241 ggcatgcggg cactccggac gggaaggcgg atatccgcca gcctacaccc gcggcgcgcg
      301 tgcggcctgc ggcatccggc gtagccgctg cgggaaaacc cgcagctcag gcgccgtcgt
      361 ccagccgctt cggtgccgtc agcggcgcgc cgtcgaacag cacggtatcg ccatcgaggc
      421 gcacacggcc ctcggcgaac cagcggatgg ccagcggata cagcttgtgt tcctcgacca
      481 gcacgcgcgc ggccagggtg tcggcatcgt cgccgggcag caccggcacc gtcgcctgca
      541 ggaacaccgg gccgccgtcg agctcgggcg tgacgaagtg gatgctgacg ccgtgctcgg
      601 tctcgccggc ggcgagcgcg cgttcgtgcg tgtgcacgcc gcggtacttc ggcagcagcg
      661 acggatggat gttgagcatg cgtccgcggt agtgctcgca gaagcccggc tccaggatgc
      721 gcatgaagcc ggcgagcacg acgagtgccg gcccgaagcc gtcgatctgc cggatcagct
      781 cggcctcgaa gctcgtgcgg tccgcgaagt ccttgtgatc gagcaccagc gccggcacgc
      841 cggcatcgcg cgcgcgctgc agcccgtaca ccccgggacg gttgctgatc accgcgcgca
      901 cctcgaccgg cagttcaccg ctcgcggcac ggtcgaggat gctctgcagg ttgctgccgc
      961 ggcccgagat cagcacgaca atgccgggtt tcgtggccga gcccatcagc ggatctccac
     1021 gcgcggctgc gcgtcgtcac cggcggcctc gatgcggccc atgacccagg ccgtctcgcc
     1081 gctggcggcg aggctcgcga gcgtggtttc gacatcggcc ggcgccacgc acacgaccat
     1141 gccgacgccg cagttgaagg tccggtacat ctcggattcg atcacaccgc cctgctgctg
     1201 cagccagcgg aagatctccg gccgctgcca gctcgccgcg tcgatgacgg cgcgcgtgcc
     1261 gtcggcgagc acgcgcggca ggttgtcggg caggccgccg ccggtgatgt gcgcgaagct
     1321 gtgcacgtcg atctgctcca gcagcttcag catcgccggc acgtagatgc gcgtcggcgc
     1381 gagcagcgcg cgcccgagcg tggtgccggc aaagggctca tcgaggctgg tgccggtgac
     1441 ggcgaggatg cgccggatca gcgaatagcc gttcgagtgc gggccgctcg acgccagacc
     1501 gatcagcacg tcgccgggcg cgacccggct gccgtcgatg atgcgctcct tctcgacgat
     1561 gccgacgcag aagccggcga gatcgtaatc ggcgccctcg tacatgcccg gcatctcggc
     1621 ggtctcgccg ccgaccagcg cgcagccggc gagcgcgcag ccatcggcga tgccctggat
     1681 gacggtcgcg gcggtgtcga catcgagctt gccggtggcg tagtaatcga ggaagaacag
     1741 cggctcggcg ccggcgaccg cgatgtcgtt ggcgcacatc gccaccaggt cgatgccgat
     1801 ggtgtcgtgc aatccggtat cgagcgcgag gcgcagcttg gtgccgacgc cgtcggtgcc
     1861 ggagaccagc accggctggc ggtagcgatc cagcggcagc tcgaacagcg cgccgaagcc
     1921 gccgaggccg ccgagcacgc cggggcgggc cgtgcgccgg gcgtgcggct tgatgcgttc
     1981 gacgagcgcc tcgccggcgt cgatatcgac gcccgcgtcg cgatagctga ggcggatggg
     2041 cggagtctgg tcgctcacgg ggatcacctg ggtcgggcgg cggacggagg ggaacaggca
     2101 gcgggccgca gccggcgtgc gaaaccgggc attctacaca tgcccggcgg caagaccgga
     2161 gccggggccg tgtggcagcg tccgacgctc atttgacagt gcgcgaggcg gctcaccaga
     2221 ctgcccggca ctcccgaccg ccgcgtttcc cgcccgctcc ctgcatgccc tccctgcgcg
     2281 cccgcgacat cccgaacctg atcaccggcc tgcgcctgct gctggtgccg ccgatcctgt
     2341 ggctgctgtg gcacgacgat cacggtgccg cgctgctgct gttcctgatc gccggcgctt
     2401 ccgatgccct cgacggtgcg ctcgcgcgcg gttttcactg gcagagccgc ctcggcggca
     2461 tcctcgaccc gatcgccgac aagtcgctgc tggtcggctg ctttctggtg ctcggtgccg
     2521 gcggcgagct gccgctgtgg ctggtggcgc tggtgctcgg ccgcgatctg gtcatcgtgc
     2581 tcggagccgc cgcctggcac tggctgatcg gcgcgctcga agcccggccc tcgctgattt
     2641 ccaagctcaa cacctgcatg cagctcgccg tggtgctcgc ggtgctgctc agtcacggcg
     2701 ccacgccgct gccggactgg ctgctgcgcc tgctcgaatg gacgatggcg ctgaccacgc
     2761 tctggagcgg catcgactac gtctggcact ggagccggct cgcgcgccgg cacgtgcgcg
     2821 gcgaggtggc cggatgaacg ccgacgagcg gcgcacctgg gcgctcgggc tggtggggct
     2881 ggtggtcgcc ggctacggcc tgtggctgct gtcgccggtg ctcgcgccct tcctgttctc
     2941 ggccgtgctc gcctatctcg gtgacccgct cgccgaccgg ctcgaacggc tcgggctgtc
     3001 gcgcacgctg tcggtggtga cggtgttcgt cagcatgttc gcgctgatcc tggtgctgct
     3061 gctgttcatc gtgccgatgc tcgaacgcca ggccacgcag ctctttgacc aggcgccggc
     3121 ggcgatcgac tggtacaacg tcgaggtgcg cggctggctg cgccagcagt tcaacctgcc
     3181 gctgccgcag ctgcgcttcg acgcgatcaa gaaggaagtc gtcaccgcgc tgcagtcggc
     3241 cggctccgac agccacgcgc tgatcgccac catcagccag tcctcggcgg tgctggtcgg
     3301 ctggacggtg acgctgctgc tggtgccggt ggtgaccttc tacctgctgc gcgactggga
     3361 cctgctggtc gaggacatcc gccacctgct gccgcgctcg gtcgagccgc aggtctcgaa
     3421 gctcgcgcgc gaatccgatg ccgtgctcgg cgccttcctg cgcgggcagt tcgcggtgat
     3481 gctcgcgctc gccgtgatct acggcggcgg cctgacgctg gtcgggctcg actacggcct
     3541 gctgatcggc ctgatcgccg ggctggtgag cttcgtgccg tacttcggct tcttcttcgg
     3601 catcgtgctc gcggggctgg cggcgatggt gcagttccac gcgccgctgc cgctgcttgc
     3661 gaccttcggc gtgttcgcgt tcgggcagct gctcgaatcc ttcatcctga cgccgcgctt
     3721 cgtcggcggc agcatcggcc tgcacccggt ggcggtgatc ttcgcgctga tggccggcga
     3781 gcacctgttc ggcttcttcg gcatcctgct ggcgctgccg gtggcctcgg tggtcatggt
     3841 gctgctgcgc cacacgcacg cgcactacgt gcagagcgaa ctctacgaaa ccccgccggc
     3901 gccgcatttc gagcctgatg agatcgtcga gtccgcggtc acgcacgaga ccatccgcgc
     3961 actcgatgcc gatgccgccg gggacggggc cggggcggcg gcggacgatg acgaagtgct
     4021 ggcgcgtgtc accaccgtcg agcgcaccga aaccgtcacc accgaaacca tcgaggcgct
     4081 gcgtccggcc ggcgcgccgc cggagcgccc ggcatgaagg acc
//
LOCUS       DAOQJH010000162         4122 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_13209_length_4122_cov_4.529137, whole genome shotgun sequence.
ACCESSION   DAOQJH010000162 DAOQJH010000000
VERSION     DAOQJH010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 4122)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4122)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4122
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13209_length_4122_cov_4.529137"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..160)
                     /locus_tag="PLQ18_04950"
     CDS             complement(<1..160)
                     /locus_tag="PLQ18_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004529023.1"
                     /note="universal genome maintenance protein;
                     Kae1/Qri7/OSGEP/YgjD family protein; in Archaea, some Kae1
                     are found as fusion proteins similar to two distinct
                     proteins in yeast that are involved in the KEOPS complex;
                     kinase associated endopeptidase 1 (Kae1) and a
                     serine/threonine protein kinase (Bud32); in Pyrococcus
                     Kae1 has atypical AP endonuclease activity and inhibits
                     the kinase activity of Bud32; the protein is essential in
                     Escherichia coli and Bacillus subtilis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HNE01035.1"
                     /translation="MRRAGARPDNPAMIVLGIETSCDETGVALYDGARGLRAHALYSQ
                     VALHAEYGG"
     gene            249..464
                     /gene="rpsU"
                     /locus_tag="PLQ18_04955"
     CDS             249..464
                     /gene="rpsU"
                     /locus_tag="PLQ18_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011235429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HNE01036.1"
                     /translation="MPVVKVKENEPFDIALRRFKRACEKAGILAEIRAREFYEKPTQE
                     RKRKQAAAVKRHQKKLAREVARRTRLY"
     gene            489..935
                     /locus_tag="PLQ18_04960"
     CDS             489..935
                     /locus_tag="PLQ18_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017463751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HNE01037.1"
                     /translation="MSLKARLTEDMKDAMRAKDKDRLATIRLILAALKQREVDERIEL
                     DDTQVLAVLDKMLKQRRESIAQFESAGRTDLAEKEAAEAAIIQTYLPAALSPAELDAL
                     IDATITATGAAGVRDMGKVMAAIKAQAQGRADMSEVSARIRTRLGG"
     gene            1046..2800
                     /gene="dnaG"
                     /locus_tag="PLQ18_04965"
     CDS             1046..2800
                     /gene="dnaG"
                     /locus_tag="PLQ18_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019464943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HNE01038.1"
                     /translation="MSGRIPQGFIDQLLARTDLVEVIGARVPLKKQGREFAACCPFHN
                     EKSPSFYVSPSKQFFHCFGCGAHGNAIGFLMDYEHLAFPEAVEELARSAGIEVPREGG
                     SAAAGQQDQYAEILHWLARADQWFRQQLRHHPERARPIEYLRGRGLAGALAAEFGIGY
                     APPERDALLQALLAEGATRKLLLAAGLVAERDCGTLVDRFRGRVMFPIRDRRGRTIAF
                     GGRVLGDGQPKYLNSPETPVFHKGRELYGLYEARMAARQIGRLVVVEGYMDVVMLAQH
                     GLRCAVATLGTSTTAEHAEKLFRITHDIVFCFDGDRAGREAAWRALENVLPQVRDGRR
                     IGFLFLPDGEDPDSLVQQEGAEAFAARLDGALPLTDYLAERLRAQVNLNTDEGAARLI
                     ELAKPLLERMPDSPARDLLVQALDRGSVLRRHTRLQQAPAGVGEFRRKVDYRKSPVRH
                     AIALLLAEPALAAEAGERSSYARIDAPGVDLLLELIDIFRDSPHLPAGAAVERYRGTA
                     RQRPLERLLAWAPEVPANEFDWQQEFRDTLARIRALGDRRRQLMDRLARGERLEAEEL
                     DWLRSVPAGNAGSPPQEP"
     gene            2897..>4122
                     /locus_tag="PLQ18_04970"
     CDS             2897..>4122
                     /locus_tag="PLQ18_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011813900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor region1.1
                     domain-containing protein"
                     /protein_id="HNE01039.1"
                     /translation="MSEQQQSQLKKLIARGKEQGFLTYAEINDHLPEDIVDPEQIEDI
                     IAMINDMGIEVHEFAPDTDTLLLAESPVATDDEDAAEEAAAALAAVDSEFGRTTDPVR
                     MYMREMGTVELLTREGEIQIAKRIEEGMNQVLAALASYPETIAELLKEYSLVQSGEAR
                     LADVIAGFVGLEEPAPAELPEDTEAALPAVEAEAAVEESEDEDGETTEAADTGPDPEE
                     AARRFAELASLLETVTASIAGAGARHPDTLAMRQQMSDRFMELKLVPKVLEMLVENLR
                     ALVDRIRAEEKIVMDACVGHAKMDRKLFITSFPEHETNPDWIDTQIAAGKKFSEALKP
                     HRDTVRSAQERLLGIQNEAGLSVNEIKDINRTMSIGEAKARRAKKEMVEANLRLVISI
                     AKKYTNRGLQFLDLIQ"
BASE COUNT          728 a         1448 c         1373 g          573 t
ORIGIN      
        1 ccccgccata ttcggcgtgc agcgccacct ggctgtagag cgcgtgcgcg cgcagaccgc
       61 gcgcgccgtc gtagagcgcg acgccggtct cgtcgcagga ggtttcgatg ccgagcacga
      121 tcatcgcggg attgtccggg cgggcgccgg cacgacgcaa gccgcttttg caatggtccg
      181 gacaaaggct tagactcgcg cgctcgccgt taccggccca gcccgctgcc tatcgaggta
      241 actccttaat gcccgtcgtg aaagtgaagg aaaacgagcc gttcgacatc gccctgcgcc
      301 gcttcaagcg cgcgtgcgag aaggccggca tccttgccga gatccgtgcc cgcgagttct
      361 acgagaagcc cacccaggaa cgcaagcgca agcaggcggc cgcggtcaag cgccaccaga
      421 agaagctcgc gcgcgaagtc gcccgtcgca cccgcctgta ctgattccgc tccggacacc
      481 cccgccgcat gtcgctgaaa gcccgcctca ccgaggacat gaaggacgcg atgcgcgcga
      541 aggacaagga ccgtctcgcg acgatccgcc tgatcctcgc cgcgctgaag cagcgcgagg
      601 tcgacgagcg catcgagctc gacgacacgc aggtcctcgc cgtcctcgac aagatgctca
      661 agcagcggcg cgagtcgatc gcgcagttcg agagcgccgg ccgcacagat ctggctgaaa
      721 aggaggccgc cgaggccgcc atcatccaga cctacctgcc ggccgcgctg agtcccgccg
      781 aactcgatgc gctgatcgac gcgaccatca ccgccaccgg cgcggccggc gtgcgcgaca
      841 tgggcaaggt catggccgcc atcaaggcgc aggcgcaggg ccgggcggac atgtccgagg
      901 tcagcgcccg catcaggacc cggctcggcg gctgatcccg cccgtgcccg atggccccga
      961 gaacccggcc cggcgccggg ttctcgcgtt ttcgcggcca ttcacgcgct agtctcgctg
     1021 caaccgcgcc gcctggaatc accgcatgtc cggtcgcatc ccgcagggct tcatcgacca
     1081 gctgctcgcc cgcaccgacc tcgtcgaggt catcggcgcg cgcgtgccgc tgaagaagca
     1141 gggacgcgaa ttcgccgcgt gctgcccgtt ccacaacgag aagtcgccgt cgttctacgt
     1201 gagtccgtcc aagcagttct tccactgctt cggctgcggc gcccacggca acgccattgg
     1261 cttcctgatg gactacgagc atctggcctt cccggaagcg gtcgaggaac tcgcgcgcag
     1321 cgccggaatc gaggtgccgc gcgagggcgg cagcgcagcg gccggccagc aggatcagta
     1381 cgccgaaatc ctgcactggc tcgcgcgcgc cgatcagtgg ttccgccagc agctgcgcca
     1441 tcacccggag cgggcccggc cgatcgaata cctgcgcggc cgcggccttg ccggcgcgct
     1501 cgccgccgag ttcggcatcg gctacgcgcc gccggagcgc gacgcgctgc tgcaggcgct
     1561 gcttgccgag ggcgcgacgc gcaagctgct gctcgccgcc ggcctggtcg ccgagcgcga
     1621 ctgtggcacg ctggtcgatc gcttccgcgg gcgcgtgatg ttcccgatcc gcgaccggcg
     1681 tggacgcacg atcgccttcg gcggtcgcgt gctcggcgac ggccagccga agtacctgaa
     1741 ttcgccggaa acgccggtgt tccacaaggg ccgcgaactc tacggcctgt acgaggcgcg
     1801 catggccgcg cgccagatcg gccggctggt ggtggtcgag ggctacatgg acgtggtcat
     1861 gctcgcgcag cacggcctgc gctgcgcggt cgccacgctc ggcacctcga ccacggccga
     1921 gcacgccgag aagcttttcc gcatcacgca cgacatcgtc ttctgcttcg acggcgaccg
     1981 cgccggacgc gaggccgcct ggcgcgcact cgagaacgtg ctgccgcagg tgcgcgacgg
     2041 ccggcgcatc ggctttctgt tcctgcccga tggcgaggac ccggattcgc tggtgcagca
     2101 ggagggcgcc gaagccttcg ccgcccggct cgatggcgcg ctgccgctga ccgactacct
     2161 cgccgaacgg ctgcgcgcgc aggtcaacct gaacaccgac gaaggcgcgg cacggctgat
     2221 agaactggca aagccgctgc tcgaacgcat gccggactcg ccggcgcgcg acctgctggt
     2281 gcaggcgctc gatcgcggct cggtgctgcg ccggcacacg cgcctgcagc aggccccggc
     2341 cggcgtcggc gagttccggc gcaaggtcga ttaccgcaag agcccggtgc gccacgccat
     2401 cgcgctgctg ctcgccgagc ccgcgctggc cgccgaggcc ggcgagcgat cgagctatgc
     2461 acggatcgat gcacccggcg tcgatctctt gcttgaactg attgatatct tcagggacag
     2521 cccgcatcta ccggcaggtg ccgcggtcga acgctatcgt ggaaccgccc ggcagcggcc
     2581 gctggagcgc ctgctggcct gggcaccgga ggtgccggcc aacgagttcg actggcagca
     2641 ggaatttcgc gacacgctcg cacgcatccg cgcgctcggc gaccggcgcc ggcagctgat
     2701 ggaccggctg gcgcgcggcg aacggctgga ggccgaagaa ctcgactggc tgcgcagcgt
     2761 cccggccgga aacgccggca gccccccgca ggaaccgtga attccgagcg gggtcaggaa
     2821 tttgtgatgg acttatgata ttgtgcgcga ttcgcctcaa cgggtatcgg gcctgcattc
     2881 gggtgcaaga gcagtcatga gcgaacagca gcagtcgcag ttgaagaagt tgatcgcccg
     2941 cggcaaggaa cagggcttcc tgacctatgc cgagatcaac gatcacctgc cggaagacat
     3001 cgtcgatccc gaacagatcg aagacatcat cgcgatgatc aacgacatgg gcatcgaggt
     3061 ccatgagttc gcgccggaca ccgacaccct gctgctcgcc gaatccccgg tcgccaccga
     3121 tgacgaggac gccgccgagg aagccgcggc cgcgctcgcc gccgtcgaca gcgagttcgg
     3181 ccgcaccacc gacccggtgc gcatgtacat gcgcgagatg ggcacggtcg agctgctgac
     3241 gcgcgagggc gagatccaga tcgccaagcg catcgaggaa ggcatgaacc aggtgctcgc
     3301 ggcgctcgcg agctacccgg agaccatcgc cgagctgctg aaggaatact cgctggtgca
     3361 atccggcgag gcgcggctgg ccgatgtcat cgccggcttc gtcggtctgg aggaaccggc
     3421 cccggccgag ctgcccgaag ataccgaagc cgcgctgccc gccgtcgagg ccgaggccgc
     3481 cgtcgaggaa tccgaggacg aggacggcga gaccaccgag gctgccgaca ccgggcctga
     3541 tccggaagaa gccgcacggc gctttgccga actcgcgagc ctgctcgaaa ccgtgaccgc
     3601 cagcatcgcc ggcgccggtg cccggcaccc cgacaccctg gcgatgcgcc agcagatgtc
     3661 ggatcgcttc atggaactga agctggtgcc gaaggtgctg gagatgctgg tcgaaaacct
     3721 gcgcgcgctg gtcgatcgca tccgtgccga ggaaaagatc gtcatggacg cctgcgtcgg
     3781 ccacgccaag atggaccgca agctgttcat caccagcttc ccggagcacg agaccaaccc
     3841 cgactggatc gacacgcaga tcgccgccgg caagaagttc tccgaagccc tgaagccgca
     3901 ccgcgacacc gtgcgctcgg cgcaggagcg cctgctcggc atccagaacg aggccggcct
     3961 gtcggtcaac gagatcaagg acatcaaccg cacgatgtcc atcggcgagg ccaaggcccg
     4021 ccgtgccaag aaggaaatgg tcgaggccaa cctgcgcctc gtgatctcga tcgccaagaa
     4081 gtacacgaac cgcggcctgc agttcctcga cctgatccag ga
//
LOCUS       DAOQJH010000163         4101 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_13298_length_4101_cov_4.708848, whole genome shotgun sequence.
ACCESSION   DAOQJH010000163 DAOQJH010000000
VERSION     DAOQJH010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 4101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4101
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13298_length_4101_cov_4.708848"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="PLQ18_04975"
     CDS             <1..195
                     /locus_tag="PLQ18_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017507657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE01040.1"
                     /translation="GRLFPETVELVAANERRYWEVEGIARVACGGTHLRRTGEVGVLA
                     LKRRNVGKGKERVEILLADG"
     gene            complement(344..703)
                     /locus_tag="PLQ18_04980"
     CDS             complement(344..703)
                     /locus_tag="PLQ18_04980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE01041.1"
                     /translation="MYALNAEDLERMGMASLGLAISSQRAALRAGEELSGIGIAASGA
                     YLMIWLGFVGRLLEARSPDTVSECVQRLNEDWQRRVRHDMRLWMATLLETSDRLGSLA
                     AAQPVNPIVPLRRRAAG"
     gene            complement(858..1379)
                     /gene="phaR"
                     /locus_tag="PLQ18_04985"
     CDS             complement(858..1379)
                     /gene="phaR"
                     /locus_tag="PLQ18_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018719348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis repressor PhaR"
                     /protein_id="HNE01042.1"
                     /translation="MSEARIIKKYPNRRLYDTAISSYITLEDVKRLVLERADFRVVDA
                     RSSDDITRGILLQIISEQEEQGNPIFTTEVLAHIIRFYGDTLQGMMGSYLEKSLQAFV
                     DQQHLVREQLRNLMGANPLQVISDLAEHNLSIWQKMNERLYKQGVELPRALQPGRAQE
                     TETARTDKPDGKK"
     gene            complement(1605..2786)
                     /locus_tag="PLQ18_04990"
     CDS             complement(1605..2786)
                     /locus_tag="PLQ18_04990"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003139732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HNE01043.1"
                     /translation="MEDVVIVAAARTAIGSFMGTLSTTPAAVLGAKVVQALLARTGLQ
                     PNQIDEVILGHVLTAGCGENPARQVAVNSGLPVEVPAFTINKLCGSGLKAVHLGYQSI
                     VDGDNQIVIVGGQENMSLAPHVLANSRTGARMGDWKLQDTLLLDGLVCAFNNYHMGIT
                     AENIAEKYGISREEQDAFAYASQQKAIAAIDSGRFKDQIVGIQIPQKKGEPLVFDTDE
                     FPNRKSTPEVLAKLKPAFKKDGTVTAGNASGVNDGAAALVLMTASKAAELGLKPLARI
                     AGYAKSGVDHKIMGTGPIPAVRKLMQKTGWTLDQLDLIEANEAFAAQAISVNRDLGWD
                     LSKVNVNGGAIAIGHPIGCSGARILTDLVHEMGRRDAKKGVATLCIGGGMGVALAIER
                     D"
     gene            complement(3226..3657)
                     /locus_tag="PLQ18_04995"
     CDS             complement(3226..3657)
                     /locus_tag="PLQ18_04995"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HNE01044.1"
                     /translation="MQRSEFRHFIELPVHWGDQDALGHVNNVQFFRYLESARVAYVET
                     VFGLPVRADGEGVILADLQCAFRSQLEYPVTVEVGTRIARLGRSSFDVHAAIWRQGAE
                     APAATSRGVIVWFDFRAQKPAPLPDSLRERVRAWETLRPQE"
     gene            complement(3748..>4101)
                     /gene="msrB"
                     /locus_tag="PLQ18_05000"
     CDS             complement(3748..>4101)
                     /gene="msrB"
                     /locus_tag="PLQ18_05000"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011386468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HNE01045.1"
                     /translation="PEQFAVCRQKATERPFTGRYNEHSAAGTYRCVCCGQPLFASRSK
                     FNAGCGWPSFSLPLDEAAIAEETDRSHGMVRTEVMCSNCGAHLGHVFDDGPGPDGLRY
                     CINSVSLDFEAAGKG"
BASE COUNT          631 a         1370 c         1401 g          699 t
ORIGIN      
        1 ggccggctct tcccggagac ggtcgagctc gtggccgcga acgagcggcg ctactgggaa
       61 gtcgagggga tcgcacgcgt agcctgcggc ggcacgcacc tgcgccgcac cggcgaggtc
      121 ggcgtcctgg cactgaagcg gcgcaatgtc ggcaagggca aggagcgagt cgagatcctg
      181 ctggccgatg gctgatctcg ccggaacgcg cggactgccg catcaagggc cggcctccgc
      241 aaacgacgac gcaccgcatg cgccagggca gtacggtgcg tcgcgagacc gctgcggcag
      301 cggcgggcgg gagcggaagg ctccgtcccg gagggctgcg gtttcagccg gccgcgcggc
      361 ggcgcagcgg cacgatcggg ttcaccggct gggcggcggc cagcgagccg aggcggtcgc
      421 tggtctccag cagcgtcgcc atccacaggc gcatgtcgtg gcgcacgcgg cgctgccagt
      481 cctcgttcag gcgctgcacg cattcgctga ccgtgtcggg gctgcgggct tcgagcaggc
      541 gcccgacgaa gccgagccag atcatcaggt aggcgccgct ggccgcgatg ccgatgcccg
      601 acagctcctc gcctgcgcgc agcgccgccc gctgcgagct gatggccaga ccgagactgg
      661 ccatgcccat tcgctccaga tcctcggcgt tcagcgcata catgacggct acctcttgat
      721 ggtgcgtcgc aacatttgtt gcagtgcaat atagggtttt ccctgatgag aagtcaacgg
      781 gctccagaca tgcaaaagcc gcccgaaggc ggccctgaca gtccgaaaca gaccggaact
      841 ccggaatccc tgcctgccta cttcttgccg tccggcttgt cggtgcgcgc ggtttccgtt
      901 tcctgtgcac gccccggctg cagtgcccgc ggcagctcga cgccctgctt gtacaggcgt
      961 tcgttcatct tctgccagat cgacagattg tgctcggcga ggtccgagat cacctgcagc
     1021 gggttggcgc ccatcaggtt gcgcagctgc tcgcgcacca gatgctgctg gtcgacgaaa
     1081 gcctgcaggc tcttttcgag gtagctgccc atcatcccct gcagcgtgtc gccgtagaaa
     1141 cggatgatgt gtgcgagcac ctcggtcgtg aagatcgggt ttccctgctc ctcctgctcg
     1201 ctgatgatct gcagcaggat gccgcgcgtg atgtcatcgc tggagcgggc atcgacgacg
     1261 cggaagtccg cccgttccag cacgagccgc ttgacgtctt cgagggtgat gtagctgctg
     1321 atcgccgtgt cgtagagccg gcgattcgga tacttcttga tgatgcgcgc ttcgctcatc
     1381 ggttccgacc ggttggggga cgccagggcc atccgctcgc ccttgcgccg ggtcctgcag
     1441 gggaccgcag tgggaatgag gaccacggtc tgacgccgca gtccggttgg gtccggcatt
     1501 tcctcccggg cagtcgcctg ccgtcatgcc gccgtgcagt cccgccgtcg ggaccgcgaa
     1561 acgtccgggg cggcgtgcct cgcgacacgc cgccccgacc gggatcaatc gcgctcgatc
     1621 gcgagcgcca cacccatgcc gccgccgatg cagagcgtcg cgacgccctt cttggcgtca
     1681 cggcgaccca tctcgtggac gaggtcggtc aggatgcggg cgccggagca gccgatcggg
     1741 tggccgatgg cgatcgcgcc gccgttgacg ttgaccttgg acagatccca gcccaggtca
     1801 cggttgaccg agatcgcctg cgcggcgaag gcctcgttgg cctcgatcag gtcgagctgg
     1861 tcgagcgtcc agccggtctt ctgcatcagc ttgcgcacgg ccgggatcgg gccggtgccc
     1921 atgatcttgt gatcgacacc cgacttcgcg tagccggcga tgcgcgccag cggcttcagg
     1981 cccagctcgg ccgccttgct cgcggtcatc agcaccagcg ccgcggcgcc gtcgttgacg
     2041 cccgaggcgt tgccggcggt gaccgtgccg tccttcttga acgccggctt cagcttggcg
     2101 agcacttcgg gcgtggactt gcggttcggg aactcgtcgg tgtcgaacac cagcggctca
     2161 cccttcttct gcgggatctg gatgccgacg atctgatcct tgaaccggcc gctgtcgatc
     2221 gcggcgatcg ccttctgctg cgaggcatag gcgaaggcat cctgctcctc gcgcgagatg
     2281 ccgtacttct cggcgatgtt ctcggcggtg atgcccatgt ggtagttgtt gaacgcgcag
     2341 accagaccgt cgagcagcag ggtgtcctgc agcttccagt cgcccatgcg cgcaccggtg
     2401 cgcgagttcg ccagcacgtg cggcgccagg ctcatgttct cctggccgcc gacgatgacg
     2461 atctggttgt cgccgtcgac gatcgactgg taaccgagat gcacggcctt caggccggaa
     2521 ccgcacagct tgttgatcgt gaaggccggg acctcgaccg gcaggcccga gttgacggcg
     2581 acctgacgcg cggggttctc accgcagccg gccgtcagca cgtgaccgag aatgacttcg
     2641 tcgatctggt tcggctgcag gccggtgcgg gcgagcagcg cctgcacgac cttggcaccg
     2701 aggaccgccg ccggcgtggt ggacagcgtg cccatgaacg aaccgatcgc ggtacgggcg
     2761 gcggcaacga ttacgacgtc ttccattttc aacctccgat aggagctctt gctgactttc
     2821 acggatcggg cgatccgccg atttccgcgt agtcatgatt tgggaccggt ccggaatgct
     2881 cgttctcccc ctccggcact tgcagcgccg gaaatttccg gacagggcgc ctgtttgacg
     2941 agcgcggggc gatactgcgg gccgggtcaa agcttgtcaa ctttgcccgg cagtgcagca
     3001 gcgtggtctg caaggtgcag caaacgcagg ccggacggca gcttggcgaa ctgcctcccc
     3061 gtcgccgccg cacagtgtac cgcgacgtat cgcggcgcag cacgaccggt tcaaacgggc
     3121 ggatgacgca aatcattccg cctgccgatc accggcatgg tcgatgcttc caaagagtgc
     3181 cggaagcggc accggcagag actgccggcc ggtgccggcc agcctctatt cctgcggtct
     3241 cagggtttcc caggcgcgca cgcgttcgcg cagggaatcc ggcagcggcg ccggtttctg
     3301 cgcgcggaaa tcgaaccaga cgatgacgcc gcgcgacgtg gcggccgggg cctccgcccc
     3361 ctggcgccag atcgccgcat gcacgtcgaa gctgctgcgc ccgagccggg cgatgcgggt
     3421 gccgacctcg accgtgaccg ggtattcgag ctggctgcgg aacgcgcact gcaggtcggc
     3481 gaggatcacg ccctcgccat cggcgcgcac cggcagtccg aacacggttt cgacgtaggc
     3541 gacccgggca ctttcgagat agcggaagaa ctgcacgttg ttgacgtggc cgagggcgtc
     3601 ctgatcgccc cagtgcaccg gcagttcgat gaaatgacgg aattcgctgc gctgcatgcg
     3661 tgggctccgg cggcggtgac ggggacgcac caccggtccc ggcccggtgg cgcacggcag
     3721 gcgccagcgc ctgccgtgcg cgcagcatca gccttttccg gcggcctcga aatccagcga
     3781 caccgaattg atgcaatacc gcaatccgtc cggtccggga ccgtcgtcga acacatggcc
     3841 gaggtgcgcg ccgcagttcg agcacatgac ctcggtgcgc accatgccgt ggctgcggtc
     3901 ggtctcctcg gcgatcgccg cctcgtcgag cggcagcgag aagctcggcc agccgcagcc
     3961 ggcgttgaac ttgctgcggc tcgcgaacag cggctggccg cagcagacgc agcggtaggt
     4021 gccggcggcc gaatgctcgt tgtagcgacc ggtgaacggc cgctcggtcg ccttctggcg
     4081 gcagacggcg aactgctcgg g
//
LOCUS       DAOQJH010000164         4097 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_13315_length_4097_cov_3.786987, whole genome shotgun sequence.
ACCESSION   DAOQJH010000164 DAOQJH010000000
VERSION     DAOQJH010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 4097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4097
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13315_length_4097_cov_3.786987"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..604)
                     /locus_tag="PLQ18_05005"
     CDS             complement(2..604)
                     /locus_tag="PLQ18_05005"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNE01046.1"
                     /translation="MPQLPPTTKALMLACVAMFCVQLLFGPWVEGLLALWPIGSGHFL
                     PWQLLTYAFLHGDMLHLFFNMLGLWMFGAEIEMIWGRKRYTHYLIASALAGAVVQQVF
                     VLIVPAGGPTLGASGALYGLLLAFGMMFPDRTIMPLFPPIPMKAKVFVVVFGVIELVS
                     GLSGRSGIAHFAHLGGMLGGWLMIRYWRGQPPFPPRARRR"
     gene            complement(733..820)
                     /locus_tag="PLQ18_05010"
     tRNA            complement(733..820)
                     /locus_tag="PLQ18_05010"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(784..786),aa:Leu,seq:caa)
     gene            941..1846
                     /gene="cysM"
                     /locus_tag="PLQ18_05015"
     CDS             941..1846
                     /gene="cysM"
                     /locus_tag="PLQ18_05015"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase CysM"
                     /protein_id="HNE01047.1"
                     /translation="MAGYKTVEDVIGATPLVRLQRIPGADSDRRGNVLLAKLEGNNPA
                     GSVKDRPAVSMIREAEARGDIRPGDTLIEATSGNTGIALAMAAAIKGYRMILIMPDNL
                     SLERRATMKAFGAELILVTRAQGMEGARDLALSMQAEGKGRVLDQFSNPDNPLAHYKS
                     TGPEIWRQTQGRITHFVSSMGTTGTIMGVSRYLKEVAPHVQVIGVQPADGAQIPGIRR
                     WPEAYLPKIYEAPRVDRILDVNQADAEAMTRQLAREEGLFCGISSGGAMVAALRIAAE
                     TPDALIVSIVCDRGDRYLSTGVFPD"
     gene            1878..2663
                     /locus_tag="PLQ18_05020"
     CDS             1878..2663
                     /locus_tag="PLQ18_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNE01048.1"
                     /translation="MNVLVFDIETIPDTDGARRLDAGLAGLDDAQVADAMFHLRRQET
                     GSDFIRHHLQRVVAISAVLRSGSQLRVWSLGTPQSGEAELIQRFFDGIGKYTPTLVSW
                     NGSGFDLPVLHYRALLHGVQAPRYWETGDSEPGFRYNHYLARFHWRHTDLMDVLAGYQ
                     PRACAPLDEIATLLGLPGKLGMDGSKVWDAYRAGRLDAIRAYCETDVLNTYLVWLRFE
                     LMRGNLGPEGYAGECRLLRDWLVQNPQPHFDEFRAGWTNCPGD"
     gene            2727..4073
                     /gene="rlmD"
                     /locus_tag="PLQ18_05025"
     CDS             2727..4073
                     /gene="rlmD"
                     /locus_tag="PLQ18_05025"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018718401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HNE01049.1"
                     /translation="MARRHHRKPRAPEGVFTARIDSLAHDGRGIARVDGKVVFIEGAL
                     PGETVEYRYLATRGGYDEALTTGVLEASPERVAPRCRHFGVCGGCSLQHLAADRQIAF
                     KHDQLVENLERIGRVTPAHWLAPLTGPHWGYRQKARLGVRFVPKKDKVLVGFREKHAP
                     YLADLLGCEVLHPSVGTRIEALSALIGSLSIKTRLPQIEVAVGEDGVALNFRVMDPPS
                     AEDCEKLAAFGREHGIRILLQPGNAGSTHALWPDEIADLHYRLPAYDVELAFRTQHFT
                     QVNADINRQMIARAIELLDVQPGQHVLDLFCGLGNFTLALARVTGAEGRVSGVEGEPA
                     LVEWAGRNAAANGIGNAGFHVADLTTDITAQTWRQARYDRILLDPPRSGALEMMPHVA
                     AMGASRIVYVSCHPATLARDAAELVHTHGYRLEAAGVMDMFPHTTHVESIALFVRD"
BASE COUNT          670 a         1469 c         1386 g          572 t
ORIGIN      
        1 atcagcgccg gcgggcgcgc ggcgggaacg ggggctggcc gcgccagtag cggatcatca
       61 gccagccgcc cagcatgccg cccaggtggg cgaagtgggc aatgccgctg cgcccggaca
      121 ggcccgagac cagctcgatc acgccgaaca cgaccacgaa gaccttggcc ttcatcggga
      181 tcggcgggaa cagcggcatg atcgtgcggt ccgggaacat catcccgaag gccagcagca
      241 ggccgtacag tgcgcccgat gcacccagcg tgggcccgcc cgccggcacg atgagcacga
      301 agacctgctg caccaccgcg ccggccagtg cgctggcgat caggtaatgg gtgtagcgct
      361 tgcggcccca gatcatctcg atctcggcgc cgaacatcca caggccgagc atgttgaaga
      421 acaggtgcag catgtcccca tgcaggaagg cataggtcag cagctgccag ggcaggaagt
      481 gccccgagcc gatcggccac agcgccagca ggccttcgac ccaggggccg aacagcaatt
      541 ggacgcagaa catcgcgacg cacgccagca tcagggcctt ggtggtcggg ggaagctggg
      601 gcatggggca ggctcggcga cgagggccgg cggcaatggc cggcctggaa gacgagggaa
      661 ctgcccggca ccgaccgtgg tcggcacgca tcggcagcgt ggaggacggc gcgccgcaac
      721 cgcggcgaag cctggtgccg gaggccggac tcgaaccggc acgccgtatg agggcggtgg
      781 attttgaatc cactgcgtct accgatttcg ccactccggc agggatggcg ctgtctctgg
      841 cagcgggccg cgattatggc atggcggcgc ggccggatgc gttggcctgc gctcctgtgc
      901 aggcctcggc ttgctgcgac aatgccgcgc tggaggtgtc atggctggct acaagacggt
      961 ggaagatgtg atcggcgcaa cgccgctggt gcgcctgcag cgcatcccgg gcgccgacag
     1021 cgatcggcgc ggcaatgtgc tgctcgccaa gctcgaaggc aacaacccgg ccggctcggt
     1081 caaggaccgc ccggcggtgt cgatgatccg cgaggccgag gcccgcggcg acatccgccc
     1141 cggcgacacg ctgatcgagg ccacgagcgg caacaccggc atcgcgctgg cgatggcggc
     1201 ggcgatcaag ggctaccgga tgatcctgat catgccggac aacctgtcgc tggagcgccg
     1261 ggcgacgatg aaggccttcg gcgccgaact gatcctcgtc accagagccc agggcatgga
     1321 aggcgcgcgc gatctggcgc tctcgatgca ggccgagggc aagggccgcg tgctcgacca
     1381 gttctcgaac cccgacaacc cgctcgcgca ctacaagtcc accggcccgg aaatctggcg
     1441 ccagacgcag ggccgcatca cgcacttcgt cagctccatg ggcacgaccg gcacgatcat
     1501 gggcgtgtcg cgttacctca aggaggtcgc gccgcacgtg caggtcatcg gcgtgcagcc
     1561 ggccgacggt gcgcagattc ccggcatccg ccgctggccc gaggcctatc tgccgaagat
     1621 ctacgaggcc ccgcgcgtcg atcgcatcct cgacgtcaat caggccgatg ccgaggcgat
     1681 gacccgccag cttgcgcgcg aggaaggcct gttctgcggg atttcctcgg gcggcgcgat
     1741 ggtcgcggcg ctgcgcatcg ccgccgaaac cccggacgcg ctgatcgtca gcatcgtctg
     1801 cgaccgcggc gaccggtatc tgtcgaccgg cgtgttcccg gactgaccgg acttacagta
     1861 cttacagtga agcgcccgtg aacgtcctcg tcttcgacat cgagaccatt cccgacaccg
     1921 acggcgcccg ccggcttgat gccggactcg ccggcctcga tgacgcacag gtcgccgatg
     1981 cgatgttcca tctgcgccgc caggaaaccg gctcggactt catccgccac cacctgcagc
     2041 gcgtcgtcgc gatctcggcc gtgctgcgct cgggcagcca gctgcgcgtc tggtcgctcg
     2101 gcacgccgca atccggcgag gccgagctga tccagcgctt cttcgacggc atcggcaagt
     2161 acacgccgac gctggtgagc tggaacggca gcggtttcga cctgccggtg ctgcactacc
     2221 gcgcgctgct gcacggcgtg caggcgccgc gctactggga gaccggcgat tccgaaccgg
     2281 gcttccgcta caaccactac ctcgcgcgct tccactggcg gcacaccgac ctgatggacg
     2341 tgctcgccgg ctaccagccg cgcgcctgcg cgccgctcga cgagatcgcg acgctgctcg
     2401 gcctgcccgg caagctcggc atggacggca gcaaggtctg ggacgcctac cgcgccggcc
     2461 ggctcgatgc gatccgcgcc tactgcgaga ccgacgtgct gaacacctat ctggtctggc
     2521 tgcgcttcga gctgatgcgc ggcaacctcg gccccgaggg ctacgccggc gaatgccggc
     2581 tgctgcgcga ctggctcgtc cagaacccgc agccgcattt cgacgagttc cgcgccggct
     2641 ggacgaactg ccccggggac tgacggctgt cgccgcccct gcccgccgcc ttcgtatccc
     2701 ccctccctga tccggtttcc gctccgatgg cccgtcgtca ccaccgcaaa ccccgcgcgc
     2761 cggaaggcgt gttcaccgcc cgcatcgaca gcctcgccca tgacggccgc ggcatcgccc
     2821 gcgtcgacgg caaggtcgtg ttcatcgaag gcgcgctgcc cggcgagacc gtcgagtacc
     2881 gctatctggc cacccgcggc ggctacgacg aggcgctgac caccggcgtg ctcgaagcga
     2941 gccccgagcg cgtggcgccg cgctgccggc acttcggcgt ctgcggcggc tgcagcctgc
     3001 agcatctggc ggccgaccgg cagatcgcgt tcaagcacga ccagctcgtc gagaacctcg
     3061 aacgcatcgg ccgcgtgacg cccgcgcact ggctggcgcc gctgaccggc ccgcactggg
     3121 gctaccgcca gaaggcgcgg ctcggcgtgc gcttcgtgcc gaagaaggac aaggtgctgg
     3181 tcggctttcg cgagaagcac gcgccctatc tcgccgacct gctcggctgc gaggtgctgc
     3241 acccgagcgt cggcacgcgc atcgaggcgc tgtcggcgct gatcggcagc ctcagcatca
     3301 aaacccgcct gccgcagatc gaggtcgcgg tcggcgagga cggcgtggcg ctgaacttcc
     3361 gggtcatgga cccgccgagt gccgaggact gcgaaaagct cgccgccttc ggccgcgagc
     3421 atggcatccg catcctgctg cagcccggca atgccggctc gacccatgcg ctgtggccgg
     3481 acgagatcgc cgacctgcac taccggctgc cggcgtacga cgtggaactg gcgtttcgca
     3541 cgcagcactt cacgcaggtc aacgccgaca tcaaccggca gatgatcgcg cgtgcgatcg
     3601 aactgctcga cgtgcagccg ggccagcacg tgctcgacct gttctgcggc ctcggcaact
     3661 tcacgctggc gctggcgcgc gtcaccggcg ccgaaggacg ggtcagcggc gtcgagggcg
     3721 aaccggcgct ggtcgaatgg gccgggcgca acgccgcggc caacggcatc ggcaacgccg
     3781 gcttccatgt cgccgacctg acgaccgaca tcacggcgca gacctggcgc caggcgcgct
     3841 acgaccgcat cctgctcgac ccgccgcgct ccggtgcgct ggagatgatg ccgcacgtcg
     3901 ccgcgatggg cgcctcgcgc atcgtctacg tctcctgcca cccggcaacg ctcgcgcgcg
     3961 acgccgccga actcgtgcac acgcacggct accggctcga agccgccggc gtcatggaca
     4021 tgttcccgca cacgacgcac gtcgaatcga tcgcgctgtt cgtgcgcgac tgaaaagccg
     4081 gcacggcgca ggcgcct
//
LOCUS       DAOQJH010000165         4093 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_13333_length_4093_cov_4.838286, whole genome shotgun sequence.
ACCESSION   DAOQJH010000165 DAOQJH010000000
VERSION     DAOQJH010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 4093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4093
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13333_length_4093_cov_4.838286"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..741)
                     /locus_tag="PLQ18_05030"
     CDS             complement(<1..741)
                     /locus_tag="PLQ18_05030"
                     /inference="COORDINATES: protein motif:HMM:TIGR04557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein dehydrogenase-associated SoxYZ-like
                     carrier"
                     /protein_id="HNE01050.1"
                     /translation="MQGVAVPFSSAVAPVAVLCAALLATVAHAGPGVPADPLASPQWP
                     AMQQRFFRDATVVFDDLVEVTLPPAAEDSMQVPVSARVRSGTAVDTLIAFIDFNPIPK
                     VLEYRPLDAEPYLGFRVRVQQATPIRVAARTADGVWHVGGAWVDAAGGGCTLPSANAA
                     KVREEDIGHVAARRWPLDGIGSRVKLRIAHPNDTGLISGTPAFFVEALDFTAADGRRL
                     AELRSFEPVAESPVFVLEYPHGNTLCIPT"
     gene            complement(759..1610)
                     /locus_tag="PLQ18_05035"
     CDS             complement(759..1610)
                     /locus_tag="PLQ18_05035"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HNE01051.1"
                     /translation="MKRILAAAVLAGLAVAQGVTSASADALAEVREKGVLRVAVYKDF
                     PPYSYTDAQGRMTGLDVEIGKALAGKMGVAMALVPIPASDESMEDDLRNAVWKGHYMG
                     WGKADVMLHVPVNADFQKRVDKVALFAPYYRELVAVAYDKTKIPDLNSLDVFRTEKVG
                     VEMRTVADQYLITTSNHAYINNVRHFRTVDAACEAFKAGEVPAFAAQRGQLEHCLGPD
                     RSRFVIGTVPAPDVRVFEWNVGMAVKADETGLRDALGEAMEALRKDGSLQKIFDRQAM
                     SLNTPAN"
     gene            complement(1750..3498)
                     /locus_tag="PLQ18_05040"
     CDS             complement(1750..3498)
                     /locus_tag="PLQ18_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol/ethanol family PQQ-dependent
                     dehydrogenase"
                     /protein_id="HNE01052.1"
                     /translation="MNRLALGVSLLLGVASAAVAGVTDQNLLDAGVKDNKSVLTWGID
                     YQGTRYSPLKQINADTIKDLVPAWTFSYGDEKQRGQEAQPLVYNGKIFVTASYSRIYA
                     VDAKTGKKLWVYEHRLPDGILPCCDVINRGAALYGDLVIYSTLDAQLVALNQETGKVV
                     WREKMDNYKDGYSNSAAPIVVKGLVITGVSGGEFGVVGRVDARDALTGKLVWTRPVIE
                     GHMGYTFKDGQKTEAGLTGETGKTWPGDLWQTGGGATWLGGYYDPETDSVLIGTGNPA
                     PWNAHTRPGDNLYSASSLAIDPSTGKIKWHYQTTPNDGWDFDGVNELVSFDLKKDGKT
                     IKAVGKADRNGFFYVLDRTNGKLISATPFVTKQTWATGVDLKTGRPIETGVRPGDPSK
                     SADGKKGETVFSAPSFLGGKNWMPMAYNKDTGLFYVPANEWGMDIWNEPITYKKGAAY
                     LGAGFNIKPLYDDYIGALRAIDPATGKIVWEYKNYSPLWGGVLTTAGNLVFTGTPEGF
                     LKAFDAKTGKELWSYQTGSGVVGCPVTWEMDGEQYIAVPSGWGGAVPLWGGEVAKRIS
                     YLTQGGSLWVFKLHKS"
BASE COUNT          621 a         1366 c         1419 g          687 t
ORIGIN      
        1 ggtcggtatg cacagtgtgt taccgtgagg atattcgagc acgaacaccg ggctctcggc
       61 gaccggctcg aagctgcgca gctcggcgag ccggcggccg tcggcggccg tgaagtccag
      121 cgcctcgacg aagaaggccg gcgtgccgct gatcagcccg gtgtcgttcg ggtgcgcgat
      181 gcgcagcttc acgcgcgagc cgatgccatc gagcggccag cgccgcgcgg cgacgtggcc
      241 gatgtcctcc tcgcgcacct tcgcggcgtt tgccgacggc agcgtgcagc cgccgccggc
      301 ggcatcgacc caggcgccgc cgacgtgcca gacgccatcg gccgtgcgcg cggcgacccg
      361 gatcggcgtg gcctgctgca cgcgcacgcg aaagcccaga tacggctcgg catcgagcgg
      421 ccggtattcg agcaccttcg gaatcgggtt gaagtcgatg aacgcgatca gcgtgtcgac
      481 ggccgtgccg ctgcgcacgc gcgcgctgac cggcacctgc atcgagtcct cggccgccgg
      541 cggcagcgtg acctcgacca gatcgtcgaa cacgaccgtt gcgtcgcgaa agaagcgctg
      601 ctgcatcgcc ggccactgcg gcgaggcgag cggatcggcc ggcacgccgg gaccggcgtg
      661 cgcgaccgtg gcaagcagcg ccgcgcacag gacggcgact ggcgcgacag cggatgagaa
      721 cggcacggcg acgccctgca tcgaggcccg cggcgggctc agttggccgg cgtgttcagg
      781 ctcatcgcct gacggtcgaa gatcttctgc agcgagccgt ccttgcgcag cgcctccatc
      841 gcctcgccga gtgcatcgcg cagcccggtc tcgtcggcct tgaccgccat gccgacgttc
      901 cactcgaaca cgcgcacgtc cggcgccggc accgtgccga tcacgaagcg cgagcggtcc
      961 gggccgaggc agtgttcgag ctggccgcgc tgcgccgcga aggccggcac ctcgccggcc
     1021 ttgaaggcct cgcaggcggc atcgacggtg cggaagtggc ggacgttgtt gatgtaggcg
     1081 tggttcgagg tcgtgatcag gtactggtcg gcgaccgtgc gcatctcgac gccgaccttc
     1141 tcggtcctga acacgtcgag cgagttcagg tccggaatct tcgtcttgtc gtaggcgacc
     1201 gcgaccagct cgcggtagta cggcgcaaac agcgcgacct tgtcgacgcg cttctggaag
     1261 tcggcgttga ccggcacgtg cagcatcacg tcggccttgc cccagcccat gtagtggccc
     1321 ttccagacgg cgttgcgcag gtcgtcctcc atcgattcgt cggaggcagg gatcggcacc
     1381 agcgccatcg cgacgcccat cttcccggca agcgccttgc cgatctcgac atccagaccg
     1441 gtcatgcggc cctgggcgtc ggtgtaggaa tacgggggaa agtccttgta gacggcgacg
     1501 cgcagcacgc ccttctcgcg cacttcggcg agcgcatcgg cactggccga ggtgacgccc
     1561 tgggcaacgg cgaggccggc gagcacggcg gcggcgagga tacgtttcat gtcaggctcc
     1621 cgggagatcg gcgcagcggc gggccggtca tcccggcgcg ccggcacggc tgagacggcg
     1681 tccctgcaac agaaagcggg gtgcggcagt cgcgccgcac cccgttcacg cccgcgtgta
     1741 accggacgat caggacttgt gcagcttgaa cacccagagg ctgccgccct gcgtcagata
     1801 gctgatgcgc ttggcgacct cgccacccca cagcggcacc gcaccacccc agcccgaggg
     1861 cacggcgatg tactgctcgc cgtccatctc ccaggtgacc gggcagccga ccacgccgga
     1921 gccggtctgg tagctccaga gctccttgcc ggtcttcgcg tcgaaggcct tcaggaagcc
     1981 ttccggcgtg ccggtgaaca ccaggttgcc ggcggtggtc agcacgccgc cccagagcgg
     2041 ggagtagttc ttgtattccc agacgatctt gccggtggcc ggatcgatcg cgcgcagcgc
     2101 gccgatgtag tcgtcataca gcggcttgat gttgaagccg gcacccaggt aggccgcacc
     2161 cttcttgtag gtgatcggct cgttccagat gtccatgccc cactcgttgg ccggcacgta
     2221 gaacaggccg gtgtccttgt tgtaggccat cggcatccag ttcttgccac cgaggaagga
     2281 cggcgccgag aacacggtct cgcctttctt gccgtcggcg ctcttggacg gatcgcccgg
     2341 gcgcacgccg gtctcgatcg gacggccggt cttgaggtcg acgccggtcg cccaggtctg
     2401 cttggtcacg aacggcgtgg cgctgatcag cttgccgttg gtgcggtcga gcacgtagaa
     2461 gaagccgttg cggtcagcct tgccgacggc cttgatggtc ttgccgtcct tcttgaggtc
     2521 gaacgagacc agctcgttga cgccgtcgaa gtcccagccg tcgttcgggg tcgtctggta
     2581 gtgccacttg atcttgccgg tggacgggtc gatcgcgagg ctcgaagccg agtacaggtt
     2641 gtcgcccgga cgggtgtggg cgttccacgg cgccgggttg ccggtgccga tcaggaccga
     2701 gtcggtctcg ggatcgtagt agccgccgag ccaggtcgcg ccgccgccgg tctgccacag
     2761 atcgcccggc caggtcttgc cggtttcacc ggtgaggccg gcctcggtct tctggccgtc
     2821 cttgaaggtg taacccatgt ggccttcgat gaccggacgc gtccagacca gcttgccggt
     2881 cagcgcatcg cgcgcatcga cgcggccgac gacaccgaac tcgccgcccg agacgccggt
     2941 gatgaccaga cccttgacga cgatcggcgc ggccgagttc gagtagccgt ccttgtagtt
     3001 gtccatcttc tcgcgccaga cgaccttgcc ggtctcctgg ttcagcgcga cgagctgcgc
     3061 atcgagcgtc gagtagatga ccagatcacc gtagagcgcg gcaccgcggt tgatcacgtc
     3121 gcagcacggc aggatgccgt ccggcaggcg gtgctcgtag acccagagct tcttgccggt
     3181 cttggcatcg accgcgtaga tgcgcgagta cgaggcggtg acgaagatct tgccgttgta
     3241 gaccagcggc tgggcttcct ggccgcgctg cttctcgtcg ccgtacgaga aggtccacgc
     3301 cggtacgagg tccttgatcg tgtcggcgtt gatctgcttc agcgggctgt agcgcgtgcc
     3361 ctgatagtcg atgccccagg tcaggacgct cttgttgtcc ttgacgccgg catcgagcag
     3421 gttctggtcg gtgacgccgg cgacggccgc cgaagcgaca ccgagcagca gggagacacc
     3481 gagggcaagg cggttcatcg ggaatcgtgc atgcatcatg ggattgctcc tcaaggctcc
     3541 ttcgggcgtg gagattcgaa tgtcccgctt ctgtgacatc aaatgagcgg tgcatcgcac
     3601 ttgagcaagg tcaatgccat ttttttcgtg cgcgttccgt gcaattgcag caatccggta
     3661 gcgatcaatc gtttgcacgg atcgtgcgat tgccggattt ttccggtcac gcaattgccg
     3721 caatgcagca tgcgatgcgg gcgtcacagc cttgccgcac cggtggcgcg gatcgtgaca
     3781 gcgcggtaca gcctgtcccc cgcacggcac tgccccggcc ggcggctgca catgccgccc
     3841 gcacggtccg cagcgccggc agggagcggc cggcaatcat cggcgcccgg cggccggatc
     3901 gccagccggc gcctgcaggc cgggaggatt tcccgcccgc cggacgcaag gcggccgccg
     3961 ccctgcaagc acagggcgac ggccgctcgg gggacgggtg cgtccgctct tactgggcgg
     4021 gcggagcagc cggggcgacc ggagctgccg gggcggccgg ctctcccgcc ggagctggct
     4081 tgggggcgtc ggc
//
LOCUS       DAOQJH010000166         4085 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_13363_length_4085_cov_4.888586, whole genome shotgun sequence.
ACCESSION   DAOQJH010000166 DAOQJH010000000
VERSION     DAOQJH010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 4085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4085
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13363_length_4085_cov_4.888586"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1084)
                     /gene="mnmE"
                     /locus_tag="PLQ18_05045"
     CDS             complement(<1..1084)
                     /gene="mnmE"
                     /locus_tag="PLQ18_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018233151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HNE01053.1"
                     /translation="MHESADPAAAPETRVTATPDTTDTIAAIATAPGRGGVGIARISG
                     PAVPGIAEGLIARLPPPRQAVCRHFRAADGTPIDAGLALYFPAPHSYTGEAVLELQGH
                     GGPVVLDLLLARACALGARPARPGEFTERAFLNGRMDLAQAEAVADLIDAASAQAARA
                     ALASLDGVFSQRVHALADGLLELRLWVEAAIDFPEEDIDFLADGALLARADALASGLD
                     ALLAAAVQGRLLSEGMRVVIAGRPNAGKSSLLNRLAGYEAAIVTDIPGTTRDVLRERI
                     ALDGLPLHVIDTAGLRDSDDPVEQEGIRRAWQEIGRADRLLLLVEDAEGLTAADAALR
                     ARLPATLALTQVRNKIDLSGAAPGEAQ"
     gene            complement(1194..2864)
                     /gene="yidC"
                     /locus_tag="PLQ18_05050"
     CDS             complement(1194..2864)
                     /gene="yidC"
                     /locus_tag="PLQ18_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011630615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HNE01054.1"
                     /translation="MENQRLVLVIALAFTAFLIWQQWITEHAPKPAPAAVATTAPATP
                     GSAEAGRSDVPLAPGTPAATAQNSALARGARIKVTTDVYEAEIDSVGGDLRRIGLRRY
                     PESLEQREQPFVLMRDAGGEVFVAQSGLRAAKGAAPDHYAVYTAERSDYALTEGQDSL
                     VVRLDWTDASGVKVVKTYTFHRGSYVIDVAETVENGSAEDWQGVAYWQLQREAPASSG
                     GGIGQTYTYTGGIVSTPEKTYEKHSFDDLKKTPVNETVTGGWVAMIQHYFLGALIPAS
                     QSERNVFYSLAVGGNRYVLGMSGAQLASVAPGASSTFQSKLYVGPKIQDRMAEVAPHL
                     ELTVDYGKLTILAEPLFWALKHLHALLGNWGWAIIVLTLLLKAAFYKLSETSYRSMAN
                     MRKLGPKLQSLKERYGDDKQKLNQAMMEMYRKEKINPLGGCLPILVQIPVFIALYWML
                     LESVELRQAPFMGWIQDLSVKDPYYVLPLIMGVSMFVQQKLSPAPPDPVQAKVMMAMP
                     VVFTFMFLWFPSGLVLYWVVNNCVSILQQWVITKRIEAGEDKAAATSG"
     gene            complement(2864..3091)
                     /gene="yidD"
                     /locus_tag="PLQ18_05055"
     CDS             complement(2864..3091)
                     /gene="yidD"
                     /locus_tag="PLQ18_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015150691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HNE01055.1"
                     /translation="MRTLVMLLIRAYRLLLSPLLGPRCRFHPSCSAYALEAVERFGTL
                     RGGWLALRRLGRCHPWHPGGYDPVPPSLERD"
     gene            complement(3076..3438)
                     /gene="rnpA"
                     /locus_tag="PLQ18_05060"
     CDS             complement(3076..3438)
                     /gene="rnpA"
                     /locus_tag="PLQ18_05060"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020564663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HNE01056.1"
                     /translation="MAGGARFERRARLVRKADFQAVFDRAEKSGDRYFTVLARPNDLG
                     HARLGLAISKRASKLAVVRNTIKRLVRESFRQRLAGLGGLDFVVMARPGLAEVEHPAL
                     FEALDRHWSRLVKRCARS"
     gene            complement(3444..3578)
                     /gene="rpmH"
                     /locus_tag="PLQ18_05065"
     CDS             complement(3444..3578)
                     /gene="rpmH"
                     /locus_tag="PLQ18_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009560916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HNE01057.1"
                     /translation="MKRTFQPSVLHRKRTHGFRARMATKNGRLVLKARRAKGRARLTP
                     "
     gene            3817..>4085
                     /locus_tag="PLQ18_05070"
     CDS             3817..>4085
                     /locus_tag="PLQ18_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006786271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA N-terminal domain-containing protein"
                     /protein_id="HNE01058.1"
                     /translation="MDDALWKLCLARLEHELSEQQLNTWVRPLQAIVEDGGLRLLAPN
                     KFVLDHVRENFLDVIRTALAGLHPQGEVAVQIEIGSSRAPEPPRR"
BASE COUNT          624 a         1391 c         1467 g          603 t
ORIGIN      
        1 cttgagcctc gccgggcgca gccccgctca ggtcgatctt gttgcgcacc tgggtcagcg
       61 ccagcgtggc cggcagccgc gcacgcagcg ccgcatccgc agccgtcaga ccctcggcgt
      121 cctcgaccag cagcagcagc cggtcggcgc gtccgatctc ctgccaggca cggcggatgc
      181 cttcctgctc gaccggatcg tcggaatcgc gcaggccggc ggtgtcgatg acgtgcagcg
      241 gcaggccgtc gagcgcgatg cgctcgcgca gcacgtcgcg cgtcgtgccc gggatgtcgg
      301 tgacgatcgc cgcctcgtag ccggccagac gattgagcag cgaggacttg ccggcattcg
      361 gccgcccggc gatgacgacg cgcatgccct cgctgagcag ccgcccctgc acggcggcgg
      421 cgagcagtgc atcgagcccg ctcgcgagcg catcggcgcg ggcgagcagc gccccgtcgg
      481 cgaggaagtc gatgtcctcc tccggaaagt cgatcgcggc ctcgacccac agccgcagtt
      541 ccagcagccc gtcggcgagc gcgtgcacgc gctgcgagaa cacgccatcg agcgaggcga
      601 gcgcggcgcg cgcggcctgg gcgctggcgg cgtcgatcag gtcggcgacc gcctcggcct
      661 gggcgagatc catgcgcccg ttcaggaagg cgcgctcggt gaactcgccg ggccgcgccg
      721 ggcgcgcgcc gagcgcgcag gcgcgcgcga gcagcaggtc gagcaccacc ggcccgccgt
      781 ggccctgcag ttcgagcacg gcctcgccgg tgtacgaatg cggcgccggg aagtacagcg
      841 ccagaccggc atcgatcggc gtgccgtcgg cggcgcggaa atgccggcag accgcctgac
      901 gcggcggcgg cagccgcgcg atcagccctt cggcgatccc gggcaccgcc ggcccgctga
      961 tgcgcgcgat gccgacgcca ccgcggcccg gcgcggtggc gatcgcggcg atcgtgtcgg
     1021 tggtgtccgg ggtggcggtg acccgggttt cgggagcggc agcgggatcg gcggattcgt
     1081 gcatcgcggc agactcggag caggaacggc ggacggcaca aatgcgcgag gggcgcggac
     1141 gcggtctgcg tcacacgccc ctcgcgggcc ttgcgggatg aacggggaaa ggctcagccg
     1201 ctcgtggccg cggccttgtc ctcgccggcc tcgatgcgct tcgtgatcac ccactgctgc
     1261 aggatcgaca cgcagttgtt gacgacccag tacagcacca gaccggacgg gaaccacagg
     1321 aacatgaacg tgaagaccac cggcatcgcc atcatgacct tggcctgcac cggatcgggc
     1381 ggggccggcg acagcttctg ctgcacgaac atcgacacgc ccatgatcag cggcagcacg
     1441 tagtacgggt ccttgaccga gaggtcctgg atccagccca tgaacggcgc ctgccgcagc
     1501 tcgacgcttt cgagcagcat ccagtacagc gcgatgaaca ccgggatctg cacgaggatc
     1561 ggcaggcagc cgccgagcgg gttgatcttc tccttgcggt acatctccat catcgcctga
     1621 ttgagcttct gcttgtcgtc gccgtagcgt tccttcagcg actgcagctt cggcccgagc
     1681 ttgcgcatgt tggccatcga gcggtagctg gtctcggaga gcttgtagaa cgccgccttc
     1741 agcagcagcg tcagcacgat gatcgcccag ccccagttgc cgagcagcgc gtgcaggtgc
     1801 ttcagcgccc agaacagcgg ctcggcgagg atcgtcagct tgccgtagtc gacggtcagc
     1861 tccagatgcg gcgcgacctc ggccatgcgg tcctggatct tcgggccgac gtagagcttc
     1921 gactggaagg tgctgctcgc ccccggcgcg acgctcgcaa gctgcgcgcc gctcatgccg
     1981 agcacgtagc ggttgccgcc gaccgcgagc gagtagaaga cgttgcgctc cgactgcgag
     2041 gccgggatca gcgcgccgag gaagtagtgc tggatcatcg cgacccagcc gccggtgacg
     2101 gtctcgttga ccggcgtctt cttcaggtca tcgaaggaat gcttctcgta ggtcttttcc
     2161 ggcgtcgaga cgatgccgcc ggtataggtg taggtctggc cgatgccgcc gccgctgctc
     2221 gccggcgcct cgcgctgcag ctgccagtag gccacgccct gccagtcctc ggcggagccg
     2281 ttttcgacgg tctcggcgac atcgatgaca tagctgccgc ggtggaaggt gtaggtcttg
     2341 accaccttca cgccggaggc gtcggtccag tcgagccgca ccaccagcga gtcctggccc
     2401 tcggtcagcg catagtcgct gcgctcggcg gtatagacgg cgtagtgatc gggcgcggcc
     2461 cccttggcgg cccgcaggcc ggactgcgcg acgaacacct cgccgccggc atcgcgcatc
     2521 agcacgaagg gctgctcgcg ctgctccagc gattccggat agcggcgcag gccgatgcgg
     2581 cgcaggtcgc cgccgacgct gtcgatctcg gcctcgtaga catcggtcgt gaccttgatg
     2641 cgcgcaccgc gcgccagggc gctgttctgc gccgtggcgg ccggcgtgcc cggcgcaagc
     2701 ggcacgtcgc tgcgcccggc ctcggcgctg cccggcgtgg ccggggcagt ggtcgcaacc
     2761 gctgccggtg ccggtttcgg cgcgtgctcg gtgatccact gctgccagat caggaaggcc
     2821 gtgaaggcga gcgcgatgac caacacgagt ctctggtttt ccatcagtct ctctcgaggc
     2881 tcgggggaac gggatcgtag ccgcccgggt gccaggggtg gcagcgcccg aggcgccgca
     2941 gggccagcca gccgccgcgc agcgtgccga agcgttcgac ggcttcgagc gcgtacgcgg
     3001 aacagctcgg atgaaagcgg cagcgggggc cgagcagcgg actcagcagc aggcgatagg
     3061 cgcggatgag cagcatcacg agcgtgcgca tcgcttcacc agtcggctcc agtgccggtc
     3121 cagcgcttcg aagagggcgg gatgttcgac ttcggcgagc ccgggccggg ccatcacgac
     3181 gaaatcgagg ccaccgagcc cggcgaggcg ctgccggaag ctttcgcgca cgaggcgctt
     3241 gatcgtgttg cggaccacgg cgagcttcga ggcccgcttg gagatcgcga gcccgagccg
     3301 ggcatggccg aggtcgttcg gacgggccag caccgtgaag taacggtcgc cggatttttc
     3361 cgcccggtcg aagaccgcct ggaagtcggc cttgcgcacc aggcgggcgc gccgttcgaa
     3421 gcgcgcgccg ccggccacgg cgcttacggc gtcaggcgcg cacgaccctt ggcgcggcgg
     3481 gccttcagca ccaggcgacc gttcttggtg gccatgcggg cacggaaacc gtgggtacgc
     3541 ttgcggtgca ggacgctggg ctggaaggtg cgcttcatgg cggttcgatc cggttgaatt
     3601 cagtgggatt tttctggggt gtcgctggcg gcgcacgtcc ggggcggacg cggcgcgcaa
     3661 acaaggcgcg ggagattagc ggtccggccc cggcactgtc aatgcaagaa ccggtgcagg
     3721 aagcgtggac gaagcgggga ccgactgtgg ataactcggc cccgaacgca tagactccgc
     3781 ggctttcgcc cgagcctgcc gccggaggag tcccgcgtgg acgacgcgct ctggaagctc
     3841 tgcctcgccc gtctggagca cgagctgagc gaacagcaac tgaatacctg ggtgcgcccg
     3901 ctgcaggcca tcgtcgagga cggcggcctg cgcctgctcg cgccgaacaa gttcgtgctc
     3961 gaccatgtgc gcgagaactt cctcgacgtg atccgcaccg ccctcgccgg cctgcatccg
     4021 cagggcgagg tcgccgtgca gatcgagatc ggttcgagcc gcgcaccgga accgccccgg
     4081 cgcag
//
LOCUS       DAOQJH010000167         4083 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_13373_length_4083_cov_4.108242, whole genome shotgun sequence.
ACCESSION   DAOQJH010000167 DAOQJH010000000
VERSION     DAOQJH010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 4083)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4083)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4083
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13373_length_4083_cov_4.108242"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            176..2878
                     /gene="tssH"
                     /locus_tag="PLQ18_05075"
     CDS             176..2878
                     /gene="tssH"
                     /locus_tag="PLQ18_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020157155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HNE01059.1"
                     /translation="MAEISRVKLFGKLNSLGYKAVEAATVFCKMRGNPYVELVHWVHQ
                     ILQLPDSDLHRLIRQFNVNPSVLVKDLTDALDRLPRGSTSITNLSAHVEEAVERGWVY
                     GSLMFGEAQVRTGYLVVGILKTPSLRSALLGISKEFDKFKADALTDRFDEYVSGSPEQ
                     GLHATDGFQVGGGAAPGEASGAIPPAQLGKQEALNRFTTDLTEQARSGKMDPIVGRDD
                     EIRQVVDILMRRRQNNPILIGEAGVGKTAVVEGFAQRIARGDVPPSLQEVTLRALDVG
                     LLQAGASMKGEFEQRLRSVIEEVQASPKPVILFVDETHTLVGAGGAAGTGDAANLLKP
                     ALARGTLRTIGATTFAEYKKHIEKDPALTRRFQSVQVDEPDEKKAVLMMRGVASTMEA
                     HHKVQILDEALEAAVKLSHRYIPARQLPDKSVSLLDTACARVAVSLHAVPAEVDDSRK
                     RIEALDTELRIIAKEAAVGLDTAARESLCNERLAAEKARLEGLEARWNAEKALVDELL
                     ATRAALRANIGVVDGPAKGEPPAKLGEAEVAALREKLHGLQTQLAELQGEQPLILPTV
                     DYQAVASVVEDWTGIPVGRIARNEIETVLKLADTLGQRVIGQDHAMQMIARRIQTSRA
                     GLDNPNKPIGVFMLAGTSGVGKTETALALAEALYGGEQNLITINMSEYQEAHTVSSLK
                     GAPPGYVGYGEGGVLTEAVRRRPYSVVLLDEVEKAHPDVHEIFFQVFDKGWMEDGEGR
                     LIDFKNTLILLTTNAGTDLLMQLCKDPELMPEPDDIAKALREPLLKVFPPALLGRLVT
                     IPYYPLSDEMLGRIVRLQLGRIKKRVEEHRKVPFEYDDEVVKLIVSRCTELESGGRMI
                     DAILTNTMLPDISREFLERMMEGQPVTRVHVSVRDGEFAYAFDA"
     gene            2970..3359
                     /locus_tag="PLQ18_05080"
     CDS             2970..3359
                     /locus_tag="PLQ18_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNE01060.1"
                     /translation="MGNLCLFSAVTGVVLDDGRPVEGARLERSYEWRWKPESGRDETR
                     TDADGRFRLPAITRGSLLGSLLPHEPFVRQTLLIHHAGRTYQAWMFNKGEYAENGELG
                     GRPISLVCRLEAAPEHHGEVYGICEPQ"
     gene            3408..>4083
                     /locus_tag="PLQ18_05085"
     CDS             3408..>4083
                     /locus_tag="PLQ18_05085"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipase family protein"
                     /protein_id="HNE01061.1"
                     /translation="MTTLTPLQAARIARGVYTLLDQTVGEARERGDLFGCEGLFTVGE
                     ESRFTGRSGGLIACKTISGFGYLAEGEGRHQGEILVATRGTSIQLDWLSNLNIGMQIG
                     PGGLPVHAGFHEVWKSFADGLAQYLRGRNPSVIHCVGHSLGGALATLNADYFSHIRAG
                     AVRLYTFGSPRAGSLFFSNALRQRLGAEHIHRVHHAADPVPKIPLFPFLHVPADSAGY
                     PLASGNH"
BASE COUNT          682 a         1434 c         1405 g          562 t
ORIGIN      
        1 ggctggacca cctggatcgg cacgccgggg gcggccgatc cggccgacct gcagctgcag
       61 gaaagcgtca tgacggcgcc ggtcccggcg cccgcctgaa tccggacctc cgcggcccgc
      121 gccggcacgc cggggccgca tacaacacga tccaccacct tcgagggagc acgtcatggc
      181 ggaaatcagt cgggtcaagc tgttcggcaa gctcaacagc ctcggctaca aggcggtcga
      241 ggcggcgacg gtgttctgca agatgcgcgg caatccgtac gtcgagctgg tgcactgggt
      301 ccatcagatc ctgcagctgc ccgattccga cctgcacagg ctgatcaggc agttcaacgt
      361 caacccctcg gtgctggtca aggacctgac tgatgccctc gaccggctgc cgcgcggctc
      421 gacctcgatc accaacctgt cggcgcacgt cgaggaagcc gtcgagcgcg gctgggtcta
      481 cggcagcctg atgttcggcg aggcgcaggt gcgcaccggc tatctggtgg tcggcatcct
      541 gaagacgccg tcgctgcgca gcgcgctgct cggcatctcg aaggagttcg acaagttcaa
      601 ggccgacgcg ctgaccgacc gcttcgacga atacgtttcc ggctcgcccg agcagggcct
      661 gcacgcgacc gacggtttcc aggtcggcgg cggtgcggcg ccgggcgagg cgagcggtgc
      721 gatcccgccg gcgcagctcg gcaagcagga ggcgctgaac cgcttcacga ccgatctgac
      781 cgagcaggcg cgcagcggca agatggaccc gatcgtcggg cgcgacgatg aaatccgcca
      841 ggtcgtcgac atcctgatgc gccgccgcca gaacaacccg atcctgatcg gcgaggccgg
      901 cgtcggcaag acggcggtcg tcgagggctt cgcgcagcgc atcgcccgtg gcgacgtgcc
      961 gccgtcgctg caggaagtca cgctgcgcgc gctcgatgtc ggcctgctgc aggccggggc
     1021 cagcatgaag ggcgagttcg agcagcgcct gcgctcggtg atcgaggaag tgcaggccag
     1081 cccgaagccg gtgatcctgt tcgtcgacga gacgcacacg ctggtcggcg ccggcggtgc
     1141 ggccggcacc ggcgatgccg ccaacctgct caagcccgcg ctggcgcgcg gcacgctgcg
     1201 cacgatcggc gcgaccacct tcgccgagta caagaagcac atcgagaagg acccggcgct
     1261 gacccggcgc ttccagagcg tgcaggtcga tgagcccgac gagaagaagg ccgtgctgat
     1321 gatgcgcggc gtcgcctcga cgatggaggc gcaccacaag gtgcagatcc tcgacgaggc
     1381 gctggaggcc gcggtcaagc tctcgcaccg ctacatcccg gcgcgccagc tgccggacaa
     1441 gtccgtgagc ctgctcgaca ccgcctgcgc gcgcgtcgcg gtcagcctgc acgcggtgcc
     1501 ggccgaggtc gatgacagcc gcaagcgcat cgaggcgctc gatacggagc tgcgcatcat
     1561 cgcgaaggaa gccgcggtcg gcctcgacac cgcggcgcgc gaatcgctct gcaacgagcg
     1621 gctggcggcc gagaaggccc ggctcgaagg gctggaagcg cgctggaacg ccgagaaggc
     1681 gctggtcgat gagctgctcg caacgcgcgc cgcgttgcgc gccaacatcg gcgtcgtcga
     1741 cgggccggcc aaaggcgagc cgccggcgaa gctcggcgag gccgaagtcg cggcgctgcg
     1801 cgaaaagctg cacggcctgc agacgcagct cgccgagctg cagggtgagc agccgctgat
     1861 cctgccgacc gtcgattacc aggcggtcgc ctcggtggtc gaggactgga ccggcatccc
     1921 ggtcgggcgc atcgcgcgca acgagatcga gacggtgctg aagctcgccg acacgctcgg
     1981 ccagcgcgtg atcggccagg accacgcgat gcagatgatc gccagacgca tccagacctc
     2041 gcgcgccggc ctcgacaatc cgaacaagcc gatcggcgtg ttcatgctcg ccggcacctc
     2101 cggcgtcggc aagaccgaga ccgcgctggc gctcgccgag gcgctgtacg gcggcgagca
     2161 gaacctgatc acgatcaaca tgagcgagta ccaggaggcg cacaccgtct cgtcgctgaa
     2221 gggcgcgcct ccgggctacg tcggctacgg cgagggcggc gtgctgaccg aggccgtgcg
     2281 ccggcggccg tactcggtcg tgctgctcga cgaggtcgag aaggcccacc cggacgtgca
     2341 cgagatcttc ttccaggtct tcgacaaggg ctggatggag gacggcgagg gccggctgat
     2401 cgacttcaag aacacgctga tcctgctgac caccaacgcc ggcaccgacc tgctgatgca
     2461 gctctgcaag gacccggagc tgatgcccga gcccgatgac atcgccaagg cgctgcgcga
     2521 acccctgctg aaggtgttcc cgccggcgct gctcgggcgg ctggtgacga tcccgtacta
     2581 cccgctcagc gacgagatgc tcgggcgcat cgtccggctg cagctcgggc gcatcaagaa
     2641 gcgcgtcgag gagcaccgca aggtgccgtt cgagtacgac gacgaggtcg tcaaactcat
     2701 cgtctcgcgc tgcaccgagc tggaatcggg cggacgcatg atcgacgcga tcctgaccaa
     2761 caccatgctg ccggacatca gccgcgagtt cctcgaacgc atgatggaag gccagccggt
     2821 cacgcgcgtg cacgtgtcgg tgcgcgacgg cgaattcgcg tatgccttcg atgcttgagc
     2881 ggcccgcggt gcgcccgccc cgcaggcatc gtcgccgggg cgggccgctg ctgacgctgt
     2941 tgctgattca cgctcacgag gccatcgcga tgggaaacct gtgcctgttc tccgccgtca
     3001 ccggcgtcgt gctcgatgac ggccggccgg tcgagggcgc gcggctcgaa cgcagctacg
     3061 aatggcgctg gaagcccgaa agcggcaggg acgagacccg caccgacgcc gacgggcgct
     3121 tccgcctgcc ggcgatcacg cgcggctcgc tgctcggctc gctgctgccg cacgaaccct
     3181 tcgtgcgcca gaccctgctc atccaccacg ccggccggac ctaccaggcg tggatgttca
     3241 acaagggcga atacgccgag aacggcgaac tgggcggccg gccgatctcg ctggtgtgcc
     3301 ggctcgaagc ggcgccggaa caccacggcg aggtgtacgg catctgcgag ccgcaatgag
     3361 ccgcagcagc gcgccacccg cagcgcccgc tccacgaggc caagaccatg acgacgctca
     3421 cgccgctgca ggcggcccgc attgcccgcg gcgtctacac gctgctcgac cagaccgtcg
     3481 gcgaggcccg cgaacgcggt gacctgttcg gctgcgaggg cctgttcacc gtcggcgagg
     3541 aatcgcgctt caccggccgc tccggcggcc tgatcgcctg caagaccatc agcggtttcg
     3601 gctacctcgc cgagggcgag gggcgtcacc agggcgagat cctcgtcgcg acccgcggca
     3661 ccagcatcca gctcgactgg ctgtcgaacc tgaacatcgg catgcagatc ggcccgggcg
     3721 ggctgccggt gcatgcgggt ttccatgagg tctggaagtc cttcgccgac gggctcgcgc
     3781 aatacctgcg cggccgcaac ccgtcggtga tccactgcgt cggccacagc ttgggcggcg
     3841 cgctcgcgac gctgaacgcc gattacttct cgcacatccg cgccggcgcc gtgcgcctgt
     3901 acaccttcgg ctcgccgcgt gccggctcgc tgttcttttc gaacgcgctg cgccagcggc
     3961 tcggcgccga gcacatccac cgcgtccacc acgccgcgga cccggtgccg aagatcccgc
     4021 tgttcccgtt cctgcacgtg ccggccgaca gcgccggcta tccgctcgcc agcggcaacc
     4081 acg
//
LOCUS       DAOQJH010000168         4064 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_13455_length_4064_cov_4.013719, whole genome shotgun sequence.
ACCESSION   DAOQJH010000168 DAOQJH010000000
VERSION     DAOQJH010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 4064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4064
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13455_length_4064_cov_4.013719"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..480)
                     /locus_tag="PLQ18_05090"
     CDS             complement(<1..480)
                     /locus_tag="PLQ18_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020483044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE01062.1"
                     /translation="MIHGPILIVDDEPLNLAVMREVLGGLYRLAFARSGEEALRAAVR
                     HQPLLILLDVRMPDMDGYEVCRQLKADAQTAAIPVIFVSGCTDTLDETRGFEAGAVDY
                     LVKPVSPPIVRARVRAHLSLVSLERLERSYHDAIYMLGEAGHYRDNDTGVHIWRMAAY
                     "
     gene            complement(514..3774)
                     /locus_tag="PLQ18_05095"
     CDS             complement(514..3774)
                     /locus_tag="PLQ18_05095"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE01063.1"
                     /translation="MHSFWPLIPIAALLIAGGGGLYAGSELRHTQERLAAAGQERVEV
                     ATATLRATMRGAVADITYLAALPDLHGALRDNDPDGLAAVAAEYVAYMLSHRDFDQIR
                     WIDASGRERVRVDYRDGQPYVVPPKALQSKADRPYFIETMQLPAGAIHQSPLDLNVEH
                     GQIERPFKPVLRFAMPLFDAEGRRHGIVVLNYLGRVLLDAVRQTSRQQTGELMLLNGD
                     GYWLLAPEPADEWGFQLDPARTLARRAPALWSALQAQVSGSLKQDDGLWLWLGLPAHL
                     SITEGAPDEFWHRALRIPQATLVAARQHIVTQVGSVVAVLLAATALIGIGFQRTRRQL
                     AHLHERLAERAEAAEAGARAKAVFLANMSHEIRTPMNAILGLAYLLERMPLPAEAGEL
                     VRRIRRSGRTLQTILNDILDFSKIEAGRLELEHAAFRLGDVLDDLSSLMAAACADKEL
                     ELVLSAPPGVDSLTGDALRLAQVLINLTGNAIKFTEHGQVSVEVRVLEADAQRVRLRF
                     AVRDTGIGIEPARQQELFAAFVQADTSTTRRFGGTGLGLAISRRLVALMGGALEVESA
                     PGLGSTFRFTLGFERGPQLPLSVPDLAGLDVLIVDDNETSRAALCGTALQLGWKPVAL
                     DSGTAAVEHVRSHFPRDPDRSRTVIVLDWQMPGMDGLATARAIRSLTGDRREPILLMA
                     TTFSCAKLQALDDSRMIDAILSKPVTPSALHDAVVRTLCRQGAAASPAPVTSQPPRLP
                     GLRVLVVDDSDINRDVARRILAGEGARVSLASDGRQAVEWLKAHVGTVDLVLMDVQMP
                     VMDGHEATCTIRRTAGLSRLPVIALTAGAFRDQQDAALAAGMNDFIAKPFDADAAVTL
                     IARVARSAGWQPAAATGASAVSRAPPLPAEPAPVLDLPQALALWNDAAVLRRYLQRFA
                     AEYGDCAARLGAAGAAEAAAGVHRLKGTAGNLGLLALAAAAVEAEQALRAGVAPPEAC
                     APLAGALDEALTAIREYAPSELPAADEAAPGTAATPGEASHGLAALLDRMLTALDTDE
                     PDAIEPLLDELAACVPAPQIAELRTAIENFDFRRAEAAAHALALRLGLSGVIPESS"
BASE COUNT          594 a         1359 c         1509 g          602 t
ORIGIN      
        1 gtaggcggcc atgcgccaga tgtgcacgcc ggtgtcattg tcgcggtaat ggccggcttc
       61 gccgagcatg tagatcgcat cgtggtagct gcgctccagc cgctccaggc tgaccagcga
      121 cagatgcgcc cgcacgcgcg cacgcacgat cggtggtgac acgggcttga cgaggtagtc
      181 gacggcaccg gcctcgaagc cgcgggtctc gtcgagcgtg tcggtgcagc cgctgacgaa
      241 gatcaccggg atggcggcgg tctgcgcatc ggccttcaac tgccggcaga cctcgtaacc
      301 gtccatgtcc ggcatgcgca cgtcgagcag gatcagcagc ggctggtggc gcacggcggc
      361 gcgcagggct tcctcgccgc tgcgggcaaa cgcgagccgg tacaggccgc cgagcacctc
      421 gcgcatcacg gcgaggttca gcggttcatc gtcgacgatc aggatcggtc catggatcat
      481 ttcgggttcc ctcgggttct ctggggggat ttttcaggaa ctttccggta tcacgcccga
      541 cagcccgagt cgcagcgcga gggcatgggc agccgcctcg gcgcggcgga aatcgaagtt
      601 ctcgatcgcg gtgcgcagtt ccgcgatctg cggcgccggc acgcacgcgg caagctcatc
      661 gagcagcggc tcgatcgcat ccggctcgtc ggtatcgagt gccgtgagca tccggtcgag
      721 cagcgcggcc agcccgtggc tggcctcgcc gggcgtggcg gctgtgccgg gggcggcttc
      781 gtctgccgct ggcagctcgg atggtgcgta ttcgcggatg gcggtcaggg cctcatcgag
      841 ggcgccggcg agcggtgcgc aggcctcagg cggcgccacg ccggcgcgca gggcctgttc
      901 ggcctcgacg gcggcggccg cgagcgcaag caggccgagg ttgccggccg tgcccttgag
      961 ccggtgcacg ccggctgccg cctcggccgc tccggcggca ccgagccgcg cggcgcagtc
     1021 gccgtactcg gcggcgaagc gctgcagata gcggcgcagc acggccgcat cgttccagag
     1081 cgccagggcc tgcggcagat cgagcacggg cgcgggctcg gccggcagcg gcggtgcgcg
     1141 gctgacggcg gaggctccag tggcggcagc cggctgccag cctgcgctgc gcgcgacgcg
     1201 ggcgatcagg gtcacggcgg catcggcatc gaagggcttg gcgatgaagt cgttcatgcc
     1261 ggccgcgagg gcggcgtcct gctggtcgcg gaaggcgccg gcggtcagcg cgatcaccgg
     1321 cagccgggac aaacccgccg tgcgccggat cgtgcacgtg gcttcgtggc cgtccatgac
     1381 cggcatctgc acatccatca gcaccagatc gacggtaccg acgtgcgcct tcagccattc
     1441 gacggcctgg cggccgtcgc tggcgagcga gacccgggcg ccttcgccgg cgaggatgcg
     1501 ccgggcgaca tcgcggttga tgtcgctgtc atcgacgacg agcacgcgca ggcccggcag
     1561 ccgcggtggc tggctggtca ccggggcggg gctggcggcg gcgccctggc ggcagagcgt
     1621 gcgcacgacg gcatcgtgca gcgccgaagg cgtcaccggc ttgctcagga tcgcatcgat
     1681 catccggctg tcgtccaggg cctgcagctt cgcgcaggag aaggtcgtgg ccatcagcag
     1741 gatcggttca cgccggtcgc cggtgaggct gcggatggcg cgggccgtcg cgagtccgtc
     1801 catgcccggc atctgccagt cgagcacgat gacggtgcgc gaccggtccg gatcgcgcgg
     1861 gaagtggctg cggacgtgtt cgacggccgc cgtgcccgaa tcgagcgcga ccggcttcca
     1921 gccgagctgc agtgccgtgc cgcagagcgc cgcgcgcgag gtttcgttgt catcgacgat
     1981 cagcacgtcg agcccggcca gatcgggcac ggacagcggc agttgcgggc cgcgttcaaa
     2041 gccgagcgtg aagcggaagg tgctgcccag gcccggggcg gattccacct ccagtgcacc
     2101 acccatcagc gccaccagcc ggcggctgat cgcgagtccg agtccggtgc cgccgaagcg
     2161 gcgcgtcgtc gaggtgtcag cctgcacgaa ggcggcgaac agctcctgct ggcgggcggg
     2221 ctcgatgccg atgccggtgt cgcgcaccgc aaagcgcagc cgcacgcgct gcgcatcggc
     2281 ttcgagcacg cgcacctcga cgctgacctg accgtgctcg gtgaacttga tcgcgttgcc
     2341 ggtcaggttg atcagcacct gagcgaggcg cagcgcgtcg ccggtcaggc tgtcgacgcc
     2401 gggcggcgct gacagcacga gttcgagctc cttgtcggcg caggcggccg ccatcagcga
     2461 ggacaggtca tcgagcacgt caccgaggcg gaaggcggcg tgttcgagtt cgagccggcc
     2521 ggcctcgatc ttcgagaagt cgaggatgtc gttgaggatg gtctgcagcg tgcggcccga
     2581 gcggcggatg cggcgcacga gttccccggc ctcggccggc agcggcatgc gttcgagcag
     2641 ataggcgagg ccgaggatcg cgttcatcgg cgtgcggatc tcgtggctca tgttcgcgag
     2701 gaacaccgcc ttggcgcgcg caccggcctc ggccgcctcg gcgcgctcgg cgagccgctc
     2761 atgcaggtga gcgagctggc gccgggtgcg ctggaagccg atgccgatca gtgccgtggc
     2821 ggccagcagt acggcgacca cgctgccgac ctgcgtgacg atgtgctggc gggcggccac
     2881 cagcgtcgcc tgcggaatgc gcagcgccct gtgccagaac tcgtcaggcg cgccttcggt
     2941 gatgctcagg tgcgccggca ggccgagcca gagccagagg ccatcgtcct gcttcaggct
     3001 gccggagacc tgcgcctgca gggcggacca gagcgccggt gcgcgccggg ccagcgtgcg
     3061 cgccggatcg agctggaagc cccattcgtc ggcaggctcg ggtgcgagca gccagtagcc
     3121 gtcgccattg agcagcatca gctccccggt ctgctggcga ctggtctggc gtacggcatc
     3181 gagcagcacg cgaccgaggt agttgagcac gacgatgccg tggcgccggc cctcggcatc
     3241 gaacagcggc atcgcaaagc gcagcaccgg cttgaacggc cgttcgatct gcccgtgctc
     3301 gacgttcagg tcgagcggcg actgatggat cgcgccggcc ggcagttgca tcgtctcgat
     3361 gaaatacggg cggtcagcct tggactgcag ggccttgggc ggtaccacgt atggctggcc
     3421 gtcgcggtaa tcgacgcgca cgcgttcgcg gccgctcgcg tcgatccagc ggatctggtc
     3481 gaaatcccga tgcgacagca tgtacgcgac gtattcagcg gccacggctg ccagtccgtc
     3541 ggggtcgttg tcacgcaacg cgccgtgcag gtccggcagc gcggccaggt aggtgatgtc
     3601 ggcaacggca ccgcgcatcg tcgcgcgcag cgtcgccgtg gccacctcga cgcgctcctg
     3661 accggccgcc gcgaggcgtt cctgcgtgtg gcgcagttcg gagccggcat acagcccgcc
     3721 gccgccggcg atcagcagcg ccgcaatcgg gatgagcggc cagaaagagt gcatgtcagc
     3781 aagccgtcga ccggcggttc ctctgcaggc tttcggtttc cgggggcagg ccggggtgcg
     3841 tcggcaatga tcaccggcag acaccggaca ggcggcggcc cgggctgcct gcctgtcgat
     3901 cggcaaggca gaagccgccc ttccgggctg gatatcgacc gacacccctg caactgtagg
     3961 tgccgggaaa cagcttccgg accggttctg cgggccttca tggcccagcc ggcgacagca
     4021 atgcctgcgc gtgccagcat tgctgtcgcc tgtctgtaca ttaa
//
LOCUS       DAOQJH010000169         4058 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_13497_length_4058_cov_4.474394, whole genome shotgun sequence.
ACCESSION   DAOQJH010000169 DAOQJH010000000
VERSION     DAOQJH010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 4058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4058
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13497_length_4058_cov_4.474394"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..516)
                     /locus_tag="PLQ18_05100"
     CDS             complement(<1..516)
                     /locus_tag="PLQ18_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011630600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HNE01064.1"
                     /translation="MSLSSPASPANLHVVILAAGKGKRMVSDLPKVLHAVGGRPMLGH
                     VIASAQRLGAARIVVVHGHGSEQVLARFGTVPGIAWAEQAEQLGTGHAVAQALPALPA
                     DGLALVLYGDVPLVRPESLTPLLAAAAAGGIGLLTVELPDPTGYGRIVRDDGGRVLRI
                     VEEKDADAATKR"
     gene            complement(613..1038)
                     /locus_tag="PLQ18_05105"
     CDS             complement(613..1038)
                     /locus_tag="PLQ18_05105"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018717467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HNE01065.1"
                     /translation="MAMTLHVDIVSAEKQIFSGTAEMLVATAEEGELGILPRHSQMLA
                     RLKPGQVRVKLQFGEEQVFYVSGGLLEVQPHTVTVLADTAERAADLDEAKAIEAKRRA
                     EEILAARSDEISLARAQAELAEAVAQLQAIEKLRKLRKA"
     gene            complement(1113..2489)
                     /gene="atpD"
                     /locus_tag="PLQ18_05110"
     CDS             complement(1113..2489)
                     /gene="atpD"
                     /locus_tag="PLQ18_05110"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_254241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HNE01066.1"
                     /translation="MSSGKIVQVIGAVVDVEFPRGAVPKIYDALKIDGADLTLEVQQQ
                     LGDGIVRAIALGTSDGLRRGMVANNTGAAINVPVGKATLGRIMNVLGTPIDHQGPVGA
                     ETTWAIHRKAPAYEEQSGSTDLLETGIKVIDLLCPFAKGGKVGLFGGAGVGKTVNMME
                     LIRNIAIEHSGYSVFAGVGERTREGNDFYHEMKDSNVLDKVALVYGQMNEPPGNRLRV
                     ALTGLTMAEYFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEMGV
                     LQERITSTKKGSITSIQAVYVPADDLTDPSPATTFAHLDATVVLSRQIAELGIYPAVD
                     PLDSTSRQLDPLIIGEEHYSTARAVQGVLQRYKELKDIIAILGMDELSEEDRQVVARA
                     RKIQRFLSQPFFVAEVFTGSPGKYVSLKDSIRGFKGIIAGEYDHLPEQAFYMVGSIDE
                     AVEKAAKL"
     gene            complement(2559..3425)
                     /gene="atpG"
                     /locus_tag="PLQ18_05115"
     CDS             complement(2559..3425)
                     /gene="atpG"
                     /locus_tag="PLQ18_05115"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018233140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HNE01067.1"
                     /translation="MAGAKEIRTKIKSIKNTQKITRAMEMVAASKMRKAQERMYAARP
                     YAEKIRTVISHLAHAHTEYRPPALMDRPDVKRVGYIVISTDRGLCGGLNTNLFKATVQ
                     SMREWQAKGVESELCTVGTKAAGFFRRLGVKVAATVSHLGDTPRIEDLVGSVKVMFDR
                     FREGEIDRIYLVHNVFLNTMTQKPSVELLVPVSARVEKVDAPHRWDYLYEPDATVLLD
                     TLLVRYVESLVYQGLVENVACEMAARMIAMKSASDNAGSLIDELQLVYNKARQAAITQ
                     ELSEIVAGAAAV"
     gene            complement(3438..>4058)
                     /locus_tag="PLQ18_05120"
     CDS             complement(3438..>4058)
                     /locus_tag="PLQ18_05120"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011040935.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the alpha chain is a
                     catalytic subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HNE01068.1"
                     /translation="YVEKFTNGAVKGKTGSLTALPVIETQAGDVSAFVPTNVISITDG
                     QIFLETDLFNAGIRPAINAGLSVSRVGGAAQTKAVKKLGGGVRLALAQYRELAAFAQF
                     ASDLDDATRKQLERGQRVTELMKQKQYSPLSVGEMAISLFAADKGYLDDVPLNKIGDF
                     ESALIAYMNANQSELMKKVNETGDYNGEIEAAFAKGVEDFKANHVW"
BASE COUNT          641 a         1353 c         1329 g          735 t
ORIGIN      
        1 gcgcttcgtg gcggcatcgg cgtccttctc ctcgacgatg cgcagcaccc ggccgccgtc
       61 gtcacgcacg atgcgcccgt agccggtcgg gtccggcagt tcgaccgtca gcaggccgat
      121 gccgccggcc gcggccgccg cgagcagcgg cgtcagggat tcagggcgca ccagcggcac
      181 gtcgccgtac agcaccagcg ccagcccgtc cgccggcagc gccggcagcg cctgcgcgac
      241 cgcgtggccg gtgccgagct gctcggcctg ctcggcccag gcgatgccgg gtaccgtgcc
      301 gaagcgcgcc agcacctgct cgctgccgtg gccgtgcacg acgacgatgc gcgccgcacc
      361 gagccgctgc gcgctggcga tgacatgccc gagcatcggc cggccgccga ccgcgtgcag
      421 gaccttgggc aggtccgaaa ccatgcgctt gcccttgccg gcggcaagga tgacgacatg
      481 gaggttcgca ggacttgcgg gactcgacag gctcatcggc gggggactcc ggcggcggca
      541 cggccgcaaa agcaaacgcc gcggacaggg ccgcggcgtg ggtcgatacg ggttgccgct
      601 tgcaggcggc gatcaggcct tgcgcagctt gcgcagcttt tcgatcgcct gcagctgggc
      661 cacggcctcg gccagttcgg cctgggcgcg ggcgaggctg atctcgtcgc tgcgggcagc
      721 gaggatttcc tcggcgcggc gcttggcttc gatcgccttc gcctcgtcga ggtcggcagc
      781 acgctcggcg gtgtccgcaa gcacggtcac cgtgtgcggc tgcacttcga gcagaccgcc
      841 ggagacgtag aacacctgct cctcgccgaa ctgcagcttc acgcgcacct gaccgggctt
      901 gagccgggcc agcatctggc tgtggcgcgg caggatgccg agttcgcctt cctcggccgt
      961 cgcgaccagc atctccgcgg tgccggagaa gatctgcttc tcggcactca cgatgtccac
     1021 atgcagcgtc atcgccatga cgtcgttacc tcgtcggatc gtcgggacgc gcgccggagt
     1081 gaggatgatt cactccggca cgccgggtcg ccttacagct tggcggcctt ctcgacggct
     1141 tcgtcgatgg aaccgaccat gtagaaggcc tgctccggca ggtggtcgta ctcgccggcg
     1201 atgatgccct tgaagccgcg gatcgagtcc ttcagcgaca cgtacttgcc cggggagccg
     1261 gtgaagactt ccgcgacgaa gaacggctgc gacaggaagc gctggatctt gcgggcacgg
     1321 gcgacgacct ggcggtcttc ttccgacagc tcgtccatgc cgaggatcgc gatgatgtcc
     1381 ttcagctcct tgtagcgctg cagcacaccc tgcacggcgc gggcggtgct gtagtgctcc
     1441 tcgccgatga tcagcggatc gagctgacgc gaggtcgagt cgagcgggtc gacggccggg
     1501 tagatgccga gctcggcgat ctggcgcgac agcacgacgg tcgcgtcaag gtgcgcgaag
     1561 gtcgtcgccg gcgacgggtc ggtcaggtcg tccgcaggca cgtacacggc ctggatcgac
     1621 gtgatcgagc ccttcttggt cgaggtgatg cgctcctgca gcacacccat ttcctcggcc
     1681 agcgtcggct ggtaacccac cgccgacggc atgcgcccga gcagcgccga cacttcggta
     1741 ccggcgagcg tgtagcggta gatgttgtcg acgaacagca gcacgtcacg gccttcgtca
     1801 cggaagtact cggccatggt caggccggtc agcgcgacgc gcagacggtt gcccggcggc
     1861 tcgttcatct ggccgtaaac gagcgcgacc ttgtcgagca cgttcgagtc cttcatctcg
     1921 tggtagaagt cgttgccctc acgggtacgc tcgcccacgc ccgcgaacac ggaataaccg
     1981 gagtgctcga tcgcgatgtt gcggatcagc tccatcatgt tcacggtctt gccgacgccg
     2041 gcgccgccga acaggccgac cttgccgccc ttcgcgaacg ggcagagcag gtcgatgacc
     2101 ttgatgccgg tttcgagcag gtcggtggaa ccggactgtt cctcgtaggc cggagccttg
     2161 cggtggatcg cccaggtcgt ctcggcgccg accggaccct gatggtcgat cggggtgccg
     2221 agcacgttca tgatgcggcc cagcgtcgcc ttgccgaccg gcacgttgat cgccgcaccg
     2281 gtgttgttgg cgaccatgcc gcggcgcagg ccgtcggagg taccgagcgc gatggcgcgc
     2341 acgatgccgt cgccgagctg ctgctgcact tccagcgtca gatccgcacc gtcgatcttg
     2401 agcgcgtcat agatcttcgg caccgcaccg cgcgggaact cgacgtccac gacggcgccg
     2461 atgacttgta cgatctttcc agaactcatt agagggttcc ccttaagact ctgctgattc
     2521 tcgaagactc tcgaaacctg cgatggcagc ccgatggatc aaacggccgc ggcgcccgcc
     2581 acgatctcgg aaagctcctg cgtgattgcc gcctgacgcg ccttgttgta gacgagctgc
     2641 aattcgtcga tgaggctgcc ggcgttgtcc gaggccgact tcatcgcgat catgcgcgcc
     2701 gccatttcgc aggccacgtt ctcgacgagt ccctgataca ccagcgattc gacgtagcgg
     2761 accagcaggg tgtccagcag caccgtcgca tcgggctcgt acaggtaatc ccagcggtgc
     2821 ggagcatcga ccttttcgac gcgcgccgag accggcacca gcagctccac gctcggcttc
     2881 tgcgtcatcg tgttcaggaa gacgttatgc acgaggtaga tgcggtcgat ctcgccttcg
     2941 cggaagcggt cgaacatgac cttcacgctg ccgaccagat cctcgatccg cggcgtgtca
     3001 cccaggtgcg acacggtggc agcgaccttc acgcccagac ggcggaagaa gcccgcagcc
     3061 ttggtgccga ccgtacacag ttcgctttcc acccctttgg cctgccactc gcgcatcgac
     3121 tgcacggtgg ccttgaacag gttggtgttc aggccaccgc acagaccgcg gtccgtggag
     3181 atcacgatgt aaccgacccg cttcacgtcg ggccggtcca tcagggccgg ggggcgatac
     3241 tcggtgtgcg catgggccag gtgcgagatg acggtgcgga tcttctccgc gtagggccgg
     3301 gccgcataca tgcgttcctg agccttgcgc atcttgctcg ccgcaaccat ttccatggca
     3361 cgagtgatct tctgagtatt cttgatactc ttgatcttcg ttcgaatctc tttggcaccg
     3421 gccatgctga cacccgctta ccagacgtgg ttggccttga aatcttccac gcccttcgcg
     3481 aaggccgctt cgatttcgcc gttgtagtcg ccggtctcgt tgaccttctt catcagctcg
     3541 ctctggttcg cgttcatgta cgcgatcagc gccgattcga agtcaccgat cttgttcagc
     3601 ggcacgtcgt ccagatagcc cttgtcggcc gcaaacagcg agatcgccat ttcgccgacg
     3661 gacagcggcg aatactgctt ctgcttcatc agctcggtga cgcgctgacc gcgttcgagc
     3721 tgcttgcggg tcgcatcgtc gaggtccgag gcgaactgcg cgaaggccgc gagttcgcgg
     3781 tactgcgcga gcgccagacg cacgccgccg ccgagcttct tgaccgcctt ggtctgcgcc
     3841 gcaccgccga cgcgcgacac cgacagaccg gcgttgatcg cgggccggat accggcgttg
     3901 aacaggtcgg tttccaggaa gatctggccg tcggtgatcg agatgacgtt ggtcggcacg
     3961 aacgcggaca cgtcgccggc ctgcgtctcg atgaccggca gcgcggtgag cgagccggtc
     4021 ttgcccttga cggcgccgtt ggtgaacttc tcgacgta
//
LOCUS       DAOQJH010000170         4046 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_13551_length_4046_cov_3.934102, whole genome shotgun sequence.
ACCESSION   DAOQJH010000170 DAOQJH010000000
VERSION     DAOQJH010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 4046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4046
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13551_length_4046_cov_3.934102"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..310
                     /locus_tag="PLQ18_05125"
     CDS             <1..310
                     /locus_tag="PLQ18_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNE01069.1"
                     /translation="ELARVLASPAGLLLLDEPFAALDWLTRRRIHAELMRLLALQPRA
                     VVLVTHDIEEAVTLADRVIVLSPRPARVRCELPLDAPRPRPLAAALPAMQRILAELET
                     "
     gene            323..760
                     /locus_tag="PLQ18_05130"
     CDS             323..760
                     /locus_tag="PLQ18_05130"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017742125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HNE01070.1"
                     /translation="MPASPAFEMRHVVTFEETNLVGNVYYANHVRWQGRCRELFLHAH
                     APQLLDEVARGLVLVTTRVACEYFDELYAFDEIIVRMRAGEIGQGRVTMLFEYWRVRE
                     GAESLVAQGEQQIACLRCAEDGRHVPTGLPEVLVRALEPYRQH"
     gene            complement(831..1457)
                     /locus_tag="PLQ18_05135"
     CDS             complement(831..1457)
                     /locus_tag="PLQ18_05135"
                     /inference="COORDINATES: protein motif:HMM:NF021141.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2459 domain-containing protein"
                     /protein_id="HNE01071.1"
                     /translation="MALALLLAGCVASRPELFPPPAGAPTVAIRLVSHGWHTGLVLPR
                     AALTGRLPALAGQFVQAEWLEIGWGDLGFYTAPDQQITSGLTLQALFASRGSVLHVVG
                     LNGPPEQAFPHSDVQPVVLGEAGFAALADGIEASFAASPAVALGPGLYGDSRFYAARG
                     HYWALHTCNTWTAERLLDAGCPVTPFWALGAGNTMWQARRHCAVNAAD"
     gene            complement(1519..2721)
                     /gene="metK"
                     /locus_tag="PLQ18_05140"
     CDS             complement(1519..2721)
                     /gene="metK"
                     /locus_tag="PLQ18_05140"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HNE01072.1"
                     /translation="MSDAYLFTSESVSEGHPDKIADQISDAVLDAIIARDEDARVACE
                     TMVKTGVAIVAGEITTSAWIDLEELVRKVICGIGYTSSDVGFDGATCGVLNIIGKQSV
                     DIAMGVNRKRSEDQGAGDQGLMFGYASTETDVLMPAPICYAHRLVERQAEARKSGLLP
                     WLRPDAKSQVTCRYEDGKVVGIDAVVLSTQHNPEIAHADLREAVMELVIKHVLPAGLI
                     TKDTQFHINPTGNFVIGGPVGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSA
                     AYAGRYVAKNIVAAGLAEKCEIQVSYAIGVAQPTSISVTTFGTGKVSDARIVELVREY
                     FDLRPYAITKMLDLLHPMYQATAAYGHFGRTPYEMTWSDKELTRTFTAFPWEKTDRAA
                     ALRDAAGL"
     gene            2958..3521
                     /locus_tag="PLQ18_05145"
     CDS             2958..3521
                     /locus_tag="PLQ18_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005221377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNE01073.1"
                     /translation="MVLLHTPVCDFGLPAPDFDLPGVDGRRWTRDAVRGPAGLLVMFI
                     CNHCPYVQAVRERLVREARDLAALGIGVVAISANDPADYPEDDFAHMQAVAREFGYPF
                     PYLFDESQQVARAYGAVCTPDFFGYNRALELQYRGRLDASGRQPAPAGAVRELYEAMK
                     QVAGTGRGPAAQLPGMGCSIKWKAQGN"
     gene            3761..>4046
                     /locus_tag="PLQ18_05150"
     CDS             3761..>4046
                     /locus_tag="PLQ18_05150"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012517371.1"
                     /note="catalyzes the formation of ribose 5-phosphate and
                     xylulose 5-phosphate from sedoheptulose 7-phosphate and
                     glyceraldehyde 3-phosphate; can transfer ketol groups
                     between several groups; in Escherichia coli there are two
                     tkt genes, tktA expressed during exponential growth and
                     the tktB during stationary phase; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HNE01074.1"
                     /translation="MPSRRELANAIRALSMDAVQQANSGHPGAPMGMAEMAAALWGGP
                     LRHNPANPAWPDRDRFVLSNGHASMLLYALLHLTGYDLPIEELKRFRQLHS"
BASE COUNT          556 a         1424 c         1464 g          602 t
ORIGIN      
        1 cgaactcgcg cgcgtgctgg cgagcccggc cggcctgctg ctgctcgacg agcccttcgc
       61 cgcgctcgac tggctgacgc gccggcgcat ccacgccgag ctgatgcggc tgctggcgct
      121 gcagccgcgc gccgtcgtgc tggtcacgca cgacatcgag gaggccgtga cactcgccga
      181 tcgcgtgatc gtgctgtcgc cgcgcccggc acgtgtacgc tgcgagctgc cgctcgatgc
      241 gccgcgcccg cggccgctcg cggcggcgct gccggcgatg cagcgcatcc tcgccgaact
      301 cgaaacctga ggagccttgc cgatgcccgc gtcccccgcc ttcgagatgc gccacgtcgt
      361 caccttcgag gagaccaacc tggtcggcaa cgtctactac gccaaccacg tgcgctggca
      421 ggggcgctgc cgcgagctgt tcctgcacgc gcacgccccg caactgctcg acgaggtcgc
      481 gcgcggcctg gtgctggtca cgacgcgcgt cgcctgcgag tacttcgacg agctgtacgc
      541 cttcgacgag atcatcgtgc gcatgcgcgc cggggagatc ggtcaggggc gcgtgacgat
      601 gctcttcgag tactggcgcg tgcgcgaggg cgccgaatcc ctggttgcgc agggtgagca
      661 gcagatcgcc tgcctgcgct gcgccgaaga cggccggcac gtgccgaccg ggctgccgga
      721 ggtgctggtg cgggcgctgg aaccgtatcg ccagcactga ggccggcttg cgcgaacggc
      781 gcggctccgg ttgcgtgggg cgtggcttgc cgtgcggccg ggggcgccgt tcagtccgcg
      841 gcgttcacgg cgcagtgacg ccgcgcctgc cacatcgtgt tgccggcgcc gagcgcccag
      901 aacggcgtga ccggacagcc ggcgtcgagc aggcgttcgg ccgtccaggt gttgcaggtg
      961 tgcagcgccc agtaatggcc gcgcgctgcg tagaagcggc tgtcgccgta caggccgggg
     1021 ccgagcgcca cggccggcga ggcggcgaag ctcgcctcga tgccatcggc gagcgccgcg
     1081 aagccggcct cgccgagcac gaccggctgc acgtcggaat gcgggaaggc ctgctccggc
     1141 gggccgttga ggccgacgac gtgcagcacc gagccgcgcg acgcgaacag cgcctgcagc
     1201 gtcaggcccg aggtgatctg ctgatccggc gccgtgtaga agccgagatc gccccagccg
     1261 atctccagcc attcggcctg cacgaactgc ccggcgagcg ccggcagccg gccggtgagc
     1321 gccgcgcgcg gcagcacgag gccggtgtgc cagccgtggc tgacgaggcg gatcgccacg
     1381 gtcggcgcgc cggcgggcgg cgggaatagc tccgggcgcg aggcgacgca gccggcgagc
     1441 agcagtgcca gcgccaccgg ccagctgcag aaaagaccgg ggccgcttgc gcggccccgg
     1501 tgtggtatcg gcggaggctt acagcccggc ggcatcccgc agcgccgcgg cgcggtcggt
     1561 cttctcccac gggaaggccg tgaaggtccg cgtgagctcc ttgtcgctcc aggtcatctc
     1621 gtacggcgtg cgcccgaagt ggccgtaggc ggcggtggcc tgatacatcg ggtgcagcag
     1681 gtcgagcatc ttcgtgatcg cgtacgggcg caggtcgaag tactcgcgca cgagctcgac
     1741 gatgcgcgca tcgctgacct tgccggtgcc gaaggtcgtc accgagatcg aggtcggctg
     1801 cgcgacgccg atcgcgtagc tgacctggat ctcgcacttc tcggcgaggc cggcggcgac
     1861 gatgttcttc gcgacgtagc gcccggcgta ggcggccgag cggtcgacct tggacgggtc
     1921 cttgcccgag aacgcgccgc cgccgtggcg ggccatgccg ccgtaggtgt cgacgatgat
     1981 cttgcgtccg gtcaggccgc agtcgccgac cgggccgccg atcacgaagt tgccggtcgg
     2041 gttgatgtgg aactgcgtgt ccttggtgat cagcccggcc ggcagcacgt gcttgatcac
     2101 gagttccatg accgcctcgc gcaggtcggc atgggcgatc tcggggttgt gctgcgtcga
     2161 gagcacgacg gcgtcgatgc cgaccacctt gccgtcctca tagcggcagg tgacctgcga
     2221 cttggcatcg gggcgcagcc acggcagcag gccggacttg cgcgcttcgg cctgacgctc
     2281 gaccaggcgg tgcgcgtagc agatcggcgc cggcatcagc acgtcggtct cggtgctcgc
     2341 atagccgaac atcaggccct ggtcgccggc gccctgatcc tcggagcgct tgcggttgac
     2401 gcccatcgcg atgtcgacgg actgcttgcc gatgatgttc agcacgccgc aggtcgcgcc
     2461 gtcgaagccc acgtccgagg acgtgtagcc gatgccgcag atgaccttgc gcacgagttc
     2521 ctcaaggtcg atccaggcgc tggtcgtgat ctcgccggcg acgatggcca cgccggtctt
     2581 gaccatcgtc tcgcatgcga cgcgggcatc ctcgtcgcgg gcgatgatcg catcgagcac
     2641 ggcatccgag atctggtcgg cgatcttgtc cggatggcct tcggacacgg actcggaggt
     2701 gaagagatag gcgtcgctca tgaaacgtcc tcgggcggcg gtccgcgcag gatgtgcggc
     2761 gggcggggcg gaaagggtgg ctggcagggt agggccggga acaaaatgcg gcggatcata
     2821 ccggcccctg ccgtcctgtg catccgtgac tcgctgcagc gcagcgtttc gacacaggac
     2881 agtacaaatg cagatgatgg cagggaggcc tgccgctgct gccgcgtctg cccgttgcct
     2941 gcttccggag gttcccgatg gtcctgctgc acaccccggt ctgtgatttc ggtctgccgg
     3001 ccccggactt cgacctgccc ggcgtcgatg gccggcgctg gacgcgcgac gccgtgcgcg
     3061 gcccggccgg gctgctggtg atgttcatct gcaaccactg cccgtacgtg caggcggtgc
     3121 gcgagcgtct ggtgcgcgag gcgcgcgatc tcgccgccct cggcatcggc gtggtcgcga
     3181 tcagcgccaa cgatccggcc gactaccccg aggacgactt cgcgcacatg caggcggtcg
     3241 cgcgcgagtt cggttatccg ttcccgtacc tgttcgacga gtcgcagcag gtcgcacgcg
     3301 cctacggggc ggtctgcacg ccggacttct tcggctacaa ccgcgcgctc gaactgcagt
     3361 accgcggccg gctcgatgcg agcggccgcc agccggcccc ggccggcgcg gtgcgcgagc
     3421 tgtacgaggc gatgaagcag gtcgccggca ccggccgcgg cccggccgcc cagctgcccg
     3481 gcatgggctg ctcgatcaag tggaaggcgc agggcaactg aggagccggc cggtcgccgg
     3541 cccggcggtt tggcgggccg tggcgatttc cgtatactcg cgcgccactt gcgagccggc
     3601 gcgcgccgct cgcagggctg cgcggcgcca tatcccggcg cggcgcggtc cgtgcggcgc
     3661 ccgcgcggcg cgcggcggag gaggctgccg cgcagttgcg ttccggccat ctccgtccgc
     3721 cgacgccgat acaccccccg cgtccctacg gagacccccg atgccctcgc gcagagaact
     3781 ggcgaacgcg atccgcgccc tgagcatgga tgccgtgcag caggccaact ccggccaccc
     3841 cggcgccccg atgggcatgg ccgagatggc cgccgcgctc tggggcgggc cgctccgcca
     3901 caacccggcc aaccccgcct ggcccgaccg cgaccgcttc gtgctctcca acgggcacgc
     3961 ctcgatgctg ctctacgcgc tgctccacct gaccggctac gacctgccga tcgaggagct
     4021 caagcgcttc cgccagctcc acagca
//
LOCUS       DAOQJH010000171         4045 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_13554_length_4045_cov_3.382206, whole genome shotgun sequence.
ACCESSION   DAOQJH010000171 DAOQJH010000000
VERSION     DAOQJH010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 4045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4045
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13554_length_4045_cov_3.382206"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1246)
                     /locus_tag="PLQ18_05155"
     CDS             complement(<1..1246)
                     /locus_tag="PLQ18_05155"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNE01075.1"
                     /translation="MRPHPPPRPLHPRLLAELERIGLREARGPVPAALWQRLTEGLSR
                     HFTAWDEEQRHFDHTIEQVYAELARLGASPALRAGAERNTLLTVLNSIADGLCVLDRS
                     GCATLVNPAAVQLLGRPQEALGGRPLLPLLLGPGVEPPDYAGLLARGETWRDEEALFV
                     RGDGERIPVAWSLTPILAGENGEPGGAVLVCRDVARQRELTRSLSHRAAHDPLTGLVN
                     RSEFEQRVAQRIDDGRPHVLMFMDLDRFKQVNDGGGHAAGDALLRGLAIVLAAQIRGS
                     DTLARLGGDEFAVLLDGCPAAHGERVAEKMLAAVRDFRLDWQGQQYAVGVSIGLAALD
                     GRYPGTAEALAGADAACYAAKLAGRDCWRLHAPEATVAAAPVAGSDWGTRLRAAIHDR
                     RLALYAQPIRPLTGGGAPAPADA"
     gene            complement(1280..1744)
                     /locus_tag="PLQ18_05160"
     CDS             complement(1280..1744)
                     /locus_tag="PLQ18_05160"
                     /inference="COORDINATES: protein motif:HMM:NF024865.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNE01076.1"
                     /translation="MNPPSPPDRSSSDDDHGFPSPEATEFAFYAAIEAADLDGMRRVW
                     EPGEACTCVHPLGPRLQGRPQVLEGWKRIFGSGTRLRFTIGSVRELDGGELRVRLVQE
                     HITVLDADEQPAQPVLATNVYRRGSDGHWRMVLHHASPGRAIAIGGPGGRLH"
     gene            2096..2614
                     /gene="fabA"
                     /locus_tag="PLQ18_05165"
     CDS             2096..2614
                     /gene="fabA"
                     /locus_tag="PLQ18_05165"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019171 -
                     3-hydroxyacyl-[acyl-carrier-protein] dehydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     FabA"
                     /protein_id="HNE01077.1"
                     /translation="MSTRQNSYTYEELLACGRGELFGPGNAQLPLPQMLMVDRILSIT
                     EDGGEFGKGQIIAELDVRPDLWFFGCHFTGDPVMPGCLGLDAMWQLVGFYLGWMGGPG
                     RGRALGCGEVKFSGQVLPSAKVITYRISMKRVILRKLVMGIADAVMEVDGRPIYSGKD
                     LRVGLFTSTDGF"
     gene            2625..3845
                     /gene="fabB"
                     /locus_tag="PLQ18_05170"
     CDS             2625..3845
                     /gene="fabB"
                     /locus_tag="PLQ18_05170"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019867681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HNE01078.1"
                     /translation="MRRVVVTGIGIVSSIGVNRHEVLAALREGRSGIEFSERYRELGL
                     RSQVHGPIRIEKEALIDRKVLRFMGDAAAFNYLAMREAIEDAGLPPGMVSNERTGLIV
                     GSGGGSPEHQVDAVDLLRTKGVRRVGPYMVTRTMSNTTSACLATPFRIRGVNYSISSA
                     CATSAHCIGNAAELIQWGKQDVMFAGGGEEVHANMTVLFDAMGALSSKYNDTPTRASR
                     AYDADRDGFVISGGGGVVVLEALDHALARGAKIYAEITGYGATSDGYDMVAPSGEGAV
                     RCMRQAMATCPTPIDYINAHGTSTPLGDTRELDAVRETFAGGRVPYISSTKSLTGHAL
                     GAAGVNEAIYSLLMLEHDFVAASANIERLDPQAEGLPIALERIDNAGLNTVMSNSFGF
                     GGTNATLIMSRYRG"
BASE COUNT          592 a         1399 c         1463 g          591 t
ORIGIN      
        1 cggcatcggc cggcgccggt gcgcccccgc cggtcaaggg ccggatcggc tgcgcgtaca
       61 gcgccaggcg ccggtcatgg atcgccgcac gcagccgggt tccccagtcg ctgccggcca
      121 ccggcgctgc cgcgaccgtg gcctcggggg cgtgcagccg ccagcagtcg cgcccggcga
      181 gcttggccgc gtagcaggcg gcatcggcac cggccagggc ttcggccgtg cccggatagc
      241 ggccgtcgag cgcggcaagc ccgatgctga cgccgaccgc gtactgctgg ccctgccagt
      301 cgagccggaa gtcgcgcacg gccgcgagca ttttttcggc gacgcgctcg ccgtgggcgg
      361 ccggacagcc gtcgagcagc acggcgaatt catcgccccc gagccgggcg agggtgtcgg
      421 agccgcggat ctgggccgcg agcacgatgg cgagcccgcg cagcagcgca tcgccggcgg
      481 catgaccgcc accgtcgttg acctgcttga agcggtcgag gtccatgaac atcagcacgt
      541 gcgggcggcc gtcgtcgatg cgctgcgcga cgcgctgttc gaactcgctg cggttgacga
      601 ggccggtcag cggatcgtgc gcggcccggt gcgacaggct gcgcgtcagc tcgcgctggc
      661 gcgccacgtc gcgacagacc agcaccgccc cgccgggctc gccattctcg ccggcgagga
      721 tcggggtcag cgaccacgcc accgggatgc gctcgccgtc gccgcgcacg aacagggctt
      781 cctcgtcacg ccaggtttca ccgcgcgcga gcaggccggc gtagtcgggc ggctcgaccc
      841 cggggccgag cagcagcggc agcagcggcc ggccgccgag cgcctcctgc ggccggccga
      901 gcagctgcac ggcggccggg ttcaccagcg tcgcgcagcc gctgcgatcg agcacgcaca
      961 ggccgtcggc gatcgagttc agcaccgtca gcagcgtgtt gcgctcggcg ccggcgcgca
     1021 gcgccggact ggcaccgagc cgggcgagct cggcgtagac ctgctcgatg gtgtggtcga
     1081 aatgccgctg ttcctcgtcc caggccgtga agtgacggct caggccctcg gtcagccgct
     1141 gccagagggc tgccggcacc ggtccgcgcg cctcgcgcag gccgatgcgc tcgagctcgg
     1201 cgagcagccg cggatgcagc gggcggggcg gcggatgggg gcgcacggga tgaggccgga
     1261 accgggaagc gaggcgcggc tagtgcagcc gcccgcccgg tccgccgatg gcgatcgcgc
     1321 gcccgggcga ggcgtgatgc agcaccatgc gccagtggcc gtcgctgccg cgccggtaga
     1381 cgttggtggc cagcaccggc tgcgccggct gctcgtcggc atcgagcacg gtgatgtgct
     1441 cctgcacgag gcgcacgcgc agttcaccgc cgtcgagctc gcgcacgctg ccgatcgtga
     1501 agcgcaggcg cgtgccgctg ccgaagatgc gcttccagcc ttcgagcacc tgcggccggc
     1561 cctgcaggcg cggcccgagc ggatgcacgc aggtgcaggc ctcgccgggc tcccagacgc
     1621 ggcgcatgcc gtcgagatcg gcggcttcga tggcagcgta gaaggcgaac tcggtggctt
     1681 ccggactcgg gaaaccgtgg tcgtcgtcgg agctgctgcg gtcgggaggc gagggtgggt
     1741 tcatggccgc aggacgatac ggactcgcca cgccggcgcc aagcgccggt gcccggattt
     1801 tgcgtcgtgc cggtgccgta tcgctgccgc ggtgccgccg cagcggcggc ggcggcacct
     1861 cgtctgcctg cacggcagcg tcggcggcac gcacctctgg cgccatggtg tgcgtatgta
     1921 gctgattcgt aagaattcgc cggtgggcgg ccgcgccgct tgcgggcgtt gcgcatcatc
     1981 cggaaacctg tgcaaacggc ggctgcacca gatgaaaagc cggtatactc gcgcgccttg
     2041 tcacgcccgt ggcacacgtg tcccgggccg atcctgccag agaacgccat ctcccgtgag
     2101 cacccgtcag aacagttaca cctacgaaga actcctcgcc tgtggtcgcg gcgagctgtt
     2161 cggccccggc aacgcccagc tgccgctgcc gcagatgctg atggtcgatc gcatcctcag
     2221 catcaccgag gacggtggcg aattcggcaa gggccagatc atcgccgagc tcgatgtgcg
     2281 ccccgacctc tggttcttcg gctgccactt caccggcgat ccggtcatgc cgggctgcct
     2341 ggggctcgat gccatgtggc agctggtcgg tttttacctc ggctggatgg gcggaccggg
     2401 ccgcggccgg gcgcttggct gcggcgaggt caagttctcg ggtcaggtgc tgccgagcgc
     2461 gaaggtgatc acctatcgca tcagcatgaa gcgcgtgatc ctgcgcaagc tggtcatggg
     2521 catcgccgat gccgtcatgg aggtcgacgg ccgcccgatc tactccggca aggatctgcg
     2581 ggtcgggctg ttcacttcca ccgacggctt ctgagggggc tgccatgcgt cgcgtcgtcg
     2641 tcaccggcat cggtatcgtt tcgagcatcg gcgtcaaccg ccacgaggtg ctcgccgcgc
     2701 tgcgcgaggg ccgaagcggc atcgagttca gcgagcgcta ccgggagctg ggcctgcgct
     2761 cgcaggtcca cggtccgatc cgcatcgaga aggaggcgct gatcgaccgc aaggtcctgc
     2821 gcttcatggg cgatgcggcc gcgttcaact acctggcgat gcgcgaggcg atcgaggatg
     2881 ccggcctgcc gcccggaatg gtctcgaacg agcgcaccgg cctgatcgtc ggctccggcg
     2941 gcggctcgcc ggaacaccag gtcgatgccg tcgacctgct gcgcaccaag ggcgtgcgcc
     3001 gcgtcgggcc gtacatggtc acgcgcacga tgagcaacac gacctcggcg tgcctggcaa
     3061 cgccgtttcg catccgcggc gtcaactatt cgatcagctc ggcctgcgcg accagcgcgc
     3121 actgcatcgg caatgccgcc gaactgatcc agtggggcaa gcaggacgtg atgttcgccg
     3181 gcggcggcga ggaagtgcat gcgaacatga ccgtgctgtt cgacgccatg ggcgcgctgt
     3241 cctcgaagta caacgacacg ccgacgcgcg cctcgcgcgc ttacgatgcc gatcgtgacg
     3301 ggttcgtgat ctccggcggc ggtggtgtcg tcgtgctcga agccctcgat cacgcactgg
     3361 cacgcggcgc gaaaatctac gccgagatca ccggctacgg tgcgacttcc gacggctacg
     3421 acatggtcgc cccgtccggc gagggcgcgg tgcgctgcat gcgtcaggcg atggcaacct
     3481 gcccgacgcc gatcgactac atcaacgccc acggcacctc gaccccgctc ggtgacacac
     3541 gcgagctcga tgcggtgcgc gagaccttcg ccggtggtcg cgtgccgtac atcagctcga
     3601 cgaagtcgct gaccgggcac gcgctcgggg ccgccggcgt caacgaggcg atctattcgc
     3661 tgctgatgct ggagcacgac ttcgtcgccg cctccgccaa catcgagcgg ctggacccgc
     3721 aggccgaagg tctgccgatc gcgctcgaac gcatcgacaa cgccgggctg aacacggtga
     3781 tgtcgaacag cttcgggttc ggcggcacca acgcgacgct gatcatgagc aggtatcgcg
     3841 gctgaagccc gtcagctgcc gtccgtgcga gggcggcgtc cgtttaggga cgtcgccttt
     3901 ttcattcatg caggccgctg ccaggggagg gcagggcgag gaagccggtc aggcccggca
     3961 gggaacggcc ccggatcgcc gggaagccgt caccggtttg gcgggggcag accggcacgg
     4021 ggccgcaaca cccggacgtg gccgc
//
LOCUS       DAOQJH010000172         4031 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_13610_length_4031_cov_4.480131, whole genome shotgun sequence.
ACCESSION   DAOQJH010000172 DAOQJH010000000
VERSION     DAOQJH010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 4031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4031
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13610_length_4031_cov_4.480131"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..119
                     /locus_tag="PLQ18_05175"
     CDS             <1..119
                     /locus_tag="PLQ18_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018228898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNE01079.1"
                     /translation="DIDKAEPYFRNALHQVLDGQPVEKAVTRPYGCTVKYAS"
     gene            216..983
                     /locus_tag="PLQ18_05180"
     CDS             216..983
                     /locus_tag="PLQ18_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009539788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNE01080.1"
                     /translation="MIDSPAFWLAAVPAVLIVGLSKGGFGGGLGVLGVPLLSLAVSPV
                     QAAAVLLPVLCVMDLASLWAFRHVPVARQLRKLLPGALLGILAGAWAWQHVDVAWVKL
                     IVGIEALAFTAHHVWQTRGGRQPPARAVSVPRAFGWGALAGFTSYLAHAGGPPLAACL
                     LPQRLPRAEFIGVTVFFFAVVNYTKLVPYALLGQLGDANLVTAAALLPLALVGVRLGV
                     VLHARLPEKPFYAICYAGLALTGTKLLWDALAALGGG"
     gene            1190..1900
                     /locus_tag="PLQ18_05185"
     CDS             1190..1900
                     /locus_tag="PLQ18_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017450361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HNE01081.1"
                     /translation="MNLRVLMLVTSHDRLGDGAHPTGVWAEELAVPYYAFTDAGCHVD
                     LASPEGGPVPFDPRSLETADGPLPAAVERLQADEALREKLRQSWRAAAIDMHVFDALF
                     VPGGHGAMWDLATDPAVAHLVETAFASGRIVAALCHGPAALVSAKGPDGRFLLEGKHV
                     NSFTDAEEGELGLGDTVPFLLENRLRELGAFFEGGPNWQPYAVRDANLITGQNPQSSA
                     LVAQQVLDALRAGLPAPA"
     gene            complement(2021..2824)
                     /locus_tag="PLQ18_05190"
     CDS             complement(2021..2824)
                     /locus_tag="PLQ18_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000093714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNE01082.1"
                     /translation="MNARSRNRQGFVAGSLIGTLGGLIGLGGAEFRLPVLIGVFRLGT
                     LEAVILNKAMSLVVVTAALLFRGVTIAPAALGDHLDVMLNLLAGSLAGAWCAAGQAVT
                     LPRRWLDRIILVLLIGLSLLMLLETRAGLHEAGTPLLEPGPLRLVTGVFAGFAIGVVA
                     ALLGVAGGELLIPVIVLLYGLDIRLAGSLSLMVSLPTMMVGFARYTRAEAFSVLRQEM
                     PLMGSMVLGSILGAAVGALLLGRVPTDWLTGLLGIVLLVSALRMFPHAR"
     gene            3235..>4031
                     /locus_tag="PLQ18_05195"
     CDS             3235..>4031
                     /locus_tag="PLQ18_05195"
                     /inference="COORDINATES: protein motif:HMM:NF024215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family membrane protein"
                     /protein_id="HNE01083.1"
                     /translation="MSFAQLLQRYFYSHYVHSGLRVALGVLGLTLAAWATLGPHAAAA
                     VSSGALGTSLTDLPGPLAEKRRLMPAAVVLGTLATLGVGLSAGTPWLNAAVVLGITFV
                     SALLLGWGRAALPLSVGTLFVTAMALGSHEAAADPWQHAGWFLAGAGGYLLWGLVAAR
                     AAARRTRQQALAESLHELAEYLRAKADCYVPGADLERCYAQLVHRQTLLVERQQAARD
                     LVLEELRTPADGTLLATLLAAFDIYEAVLSAHTDHALLHRTLAGADAL"
BASE COUNT          546 a         1519 c         1405 g          561 t
ORIGIN      
        1 ccgacatcga caaggccgag ccctacttcc gcaacgccct gcatcaggtg ctcgatggcc
       61 agccggtcga gaaggccgtg acccggccct acggctgcac ggtcaagtac gcgagctgac
      121 agagcagcga tgcgcgcgcc gcgctggccg gcgcgcgcgg ccggcggcta ccgtcgcggg
      181 ctctgcaaag cagagctgcc cggagccgcc cgccgatgat cgattccccc gccttctggc
      241 tcgccgccgt gccggcggtg ctgatcgtcg gcctgtccaa gggcggcttc ggcggcggac
      301 tcggcgtgct cggcgtgccg ctgctgtcgc tggcggtgtc gccggtgcag gcggcggccg
      361 tgctgctgcc ggtgctgtgc gtgatggatc tggcaagcct ctgggccttc cggcacgtgc
      421 cggtcgcccg gcagctgcgc aagctgctgc cgggggcgct gctcggcatc ctcgccggtg
      481 cctgggcctg gcagcacgtt gacgttgcct gggtgaagct gatcgtcggc atcgaggcgc
      541 tggccttcac cgcgcatcac gtctggcaga cgcgcggcgg ccgccagccg ccggcgcgtg
      601 ccgtgtcggt gccgcgtgcg ttcggctggg gggcgctggc cggcttcacg agctatctcg
      661 cccacgccgg cggcccgccg ctcgccgcct gcctgctgcc gcagcgcctg ccgcgcgccg
      721 agttcatcgg cgtgaccgtg ttcttcttcg cggtggtcaa ttacaccaag ctcgtgccct
      781 acgcgctgct cggccagctc ggcgacgcca acctcgtcac cgccgcggcg ctgctgccgc
      841 tggccctcgt cggcgtacgc ctcggcgtcg tgctgcatgc gcggctgccg gaaaagccgt
      901 tctacgcgat ctgctacgcc gggctggcgc tgaccggcac gaagctgctg tgggatgcgc
      961 tggcggcgct gggcggcggc tgaggacacc gccgcccgca tcgggaatcc ccggtgcccc
     1021 cggtgacctc aggaccgtca tggccctgcc gccgccccgc gcctgcacac ccgcagcgcc
     1081 cgggcatcct gctcccgtcc gcactccggc cccggtccgc tgaaccgccc tgccggccgg
     1141 cgactctgct gcctgtgcct gaccacggga acaccagagg agacccgaca tgaacctgcg
     1201 cgtgctgatg ctcgtgactt cgcatgaccg cctcggcgac ggcgctcacc cgaccggcgt
     1261 ctgggccgag gaactggccg tgccctacta cgccttcacc gatgccggct gccacgtcga
     1321 cctcgcctcg ccggagggcg ggccggtgcc gttcgacccg cgcagcctcg agaccgccga
     1381 cggcccgctg cccgcggccg tagaacgcct gcaggccgat gaggcgctcc gggagaagct
     1441 ccgccagagc tggcgcgccg ctgccatcga catgcacgtg ttcgatgcgc tgttcgtgcc
     1501 cggcggtcac ggcgcgatgt gggacctcgc caccgacccg gcggtcgcgc atctggtcga
     1561 aacggcgttt gcgtccgggc gcatcgtcgc cgcgctctgc cacggaccgg cggccctggt
     1621 ctcggccaag ggcccggacg gacgcttcct gctcgaaggc aagcacgtca acagcttcac
     1681 cgacgccgag gaaggcgagc tcggcctcgg cgacaccgtg cccttcctgc tggaaaaccg
     1741 gctgcgcgaa ctcggcgcct tcttcgaagg cggtccgaac tggcagccgt acgcggtgcg
     1801 cgacgccaac ctgatcaccg gccagaaccc gcagtcctcg gcgctggtcg cgcagcaggt
     1861 gctcgatgcc ctgcgtgcgg ggctgcccgc tccggcctga gccgtaccga ggcatccgca
     1921 ggaaatgtcc gcgccgcccg cgccgacggc acggcgcctg ccgccaaccc gtgtccgcaa
     1981 gcgccccggc ggctgcggaa ggctccggtc cgcgcccctg tcagcgcgcg tgcgggaaca
     2041 tcctgagggc cgaaaccagc aggacgatgc cgagcagccc cgtcagccag tcggtcggca
     2101 cccgcccgag cagcagggct cccacggccg cgcccaggat cgagccgagc accatcgagc
     2161 ccatcagcgg catttcctga cgcaacaccg agaaggcctc ggcgcgggtg tagcgggcga
     2221 agccgaccat catggtcggc aggctgacca tcagcgacag gctgcccgcg agcctgatgt
     2281 cgaggccgta gagcagcacg atgaccggaa tcagcagctc gccccctgcc acccccagca
     2341 gcgccgcgac gacgccgatc gcgaagcctg cgaacacccc ggtcacgagg cgcagagggc
     2401 cgggttcgag cagcggtgtg ccggcttcgt gcaggccggc ccgggtttcc agcagcatga
     2461 gcagggacag cccgatcagg agcaccagga tgatgcgatc cagccagcgc cggggcagcg
     2521 tgacggcctg gcctgccgcg caccaggcac cggcgagact gccggcgagc aggttcagca
     2581 tcacgtcgag atgatcgccg agcgcggccg gtgcgatcgt gacgccccgg aacagcagcg
     2641 cggcggtcac gacgaccagg ctcatggcct tgttgaggat cacggcctcc agcgtcccca
     2701 gccggaacac gccgatcagc accggcagcc ggaattcggc cccgccgagg ccgatcagtc
     2761 cgcccagggt gccgatcagg gaaccggcga cgaagccctg acggttgcgg ctccgggcgt
     2821 tcaccggcac gcccgctgac ggacggctgc atgccgcagg accggtaccg gccgctcgtt
     2881 tttgtcgcca tccgggaaat tcatctgctg cctgctgccc atgccgtcat tgcaccgatg
     2941 caccgttgcg gatgcgctga tccggtctgc gacttctgtc gggtcaaaaa tgacatgacg
     3001 gcccgcccga ggcatccccc gtgccggaac atcagccgga attccgaccg gaagctcacg
     3061 aggatcaggc agccgaggcc accggagccg gcttgagaag cgctttcagc cccgcgccgg
     3121 ctgcgtgtga tcggcatcgc gcaccggagc ctgccggaaa cggctcccgg acgccgccgg
     3181 ccccgccgcc acatagactg gcctccgccc caccgccgcc ggagaccgcc gtcgatgagc
     3241 ttcgcgcagc tgctgcagcg ctacttctac agccactacg tgcacagcgg cctgcgcgtc
     3301 gcgctcggcg tgctcgggct gacgctcgcg gcctgggcga cgctcgggcc gcacgcggcg
     3361 gcggcggtgt cctcgggagc cctcgggacg agcctgaccg acctgcccgg gccgctggcc
     3421 gagaagcgcc ggctcatgcc ggcggcggtc gtgctcggca cgctggccac actcggcgtg
     3481 ggcctcagtg ccggtacgcc gtggctcaac gccgcggtcg tgctcggcat caccttcgtc
     3541 tcggcgctgc tgctcggctg gggccgggcg gcgctgccgc tcagcgtcgg cacgctgttc
     3601 gtcaccgcga tggcgctcgg cagccacgag gcggcggccg atccctggca gcacgccggc
     3661 tggttcctgg ccggagccgg cggttacctg ctctggggcc tggtcgccgc gcgcgcggcg
     3721 gcgcggcgca cgcgccagca ggcgctggcc gagagcctgc acgagcttgc cgaatacctg
     3781 cgcgccaagg ccgactgcta cgtgccgggg gcggatctcg aacgctgcta cgcgcagctc
     3841 gtgcaccgcc agacgctgct ggtcgagcgc cagcaggcgg cgcgcgatct ggtgctggag
     3901 gagctgcgca cgccggccga cggcacgctg ctcgcgacgc tgctcgcggc cttcgacatc
     3961 tacgaggcgg tgctctcggc gcacaccgac cacgcgctgc tgcaccgcac gctggccggg
     4021 gccgatgcgc t
//
LOCUS       DAOQJH010000173         4017 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_13683_length_4017_cov_3.150177, whole genome shotgun sequence.
ACCESSION   DAOQJH010000173 DAOQJH010000000
VERSION     DAOQJH010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 4017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4017
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13683_length_4017_cov_3.150177"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..921
                     /locus_tag="PLQ18_05200"
     CDS             <1..921
                     /locus_tag="PLQ18_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HNE01084.1"
                     /translation="GGTKIELLALDADGRECLRRRVPTPQGDYAATLQAIRALVEAAE
                     QELGGRASVGLGTPGSASPRDGRMRNANSTCLNGRPLARDLEALLNRPLRFANDANCL
                     ALSEAVDGAAAGAGLVFAVILGTGVGGGLAAHGRVLNGHNGIAGEWGHNPLPWPAIGE
                     HPGPLCWCGRSGCIETWLSGPGFARAHAREHGLQQLVPAETIVARARAGDPAAATSLA
                     RYCERLARALATVVNLLDPEVIVLGGGLSRVDEIYARVPALMAPHVFADAVDTPLRPA
                     LHGDSSGVRGAAWLWNDGAAVSACQAGRGD"
     gene            1017..1358
                     /locus_tag="PLQ18_05205"
     CDS             1017..1358
                     /locus_tag="PLQ18_05205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE01085.1"
                     /translation="MADELDAGRGKLLEKLNAAFERMTTLDITTAVGEATKNPATGRY
                     EPAAGSKLLYTSIDLVGGDILNIVPADFGEGADDALRAFHQSQVASAQATVKANLDAV
                     EKLIALATGKD"
     gene            1365..2312
                     /locus_tag="PLQ18_05210"
     CDS             1365..2312
                     /locus_tag="PLQ18_05210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE01086.1"
                     /translation="MADLRQIALSIAQDLMTLEVNTVLKPGMTARKPPAYPLLLREIA
                     EKYVATLVQLDAGPAPIPGNPTADTVHIDAVHADDDSVAAVHIDAVLQDAAAPAEPPA
                     PPPPASEHDTAIGRALALIAQTFPGLAAPYGHLADAINALHPPGNAATFESGAGPFDE
                     RFAGALLWATLFGSCADVAATLLAQTPAESPARAPLRRIARESRWLQAFIGWQHLQTT
                     PGRGFDLQPEDRMRLRKLWNLGGERVVMQTTIHLDGDVLNGIDPALLLHPQFEAVKAL
                     HADGVGTALTHWRALFEAICTAFGKLGDAFSNVLDKRRG"
     gene            2287..2898
                     /locus_tag="PLQ18_05215"
     CDS             2287..2898
                     /locus_tag="PLQ18_05215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE01087.1"
                     /translation="MSSTSAAAEPPRWVAGLLDSRSGAPAFYPRPGLRALLAELLHGD
                     GAQLVTARTGADGTPDLDHVAVTTLLQLDGSVLVQIDERSLAAPDWPAAFAVHRAALG
                     IAGERLSAYGKSVATTLTSIRGIAMCTLPALASPTPMGSRVAATVCAWWGFSPETAPL
                     LATGLHVLAQAVMPMAAGFGIARLAGPWLVRALLRLGMWWVRR"
     gene            complement(2915..3529)
                     /locus_tag="PLQ18_05220"
     CDS             complement(2915..3529)
                     /locus_tag="PLQ18_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011405272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HNE01088.1"
                     /translation="MLYAFDLFGVFVFAVSGALVAARKQLDWLGALVLAAVTAVGGGT
                     LRDLLLARHPLVWFSDAHYLLTIALAVAATWVWGRQRPVPKRALLVADAAGLSLFTVV
                     GTQVALTQGQGAVIAVLLGITTGAAGGAIRDVLCAEVPLVLRQEIYATAAAAGGLLFV
                     LLTGLGLPAAAAALVAMAAVFALRLAAIYRDWHLPRYRLRAEGE"
     gene            complement(3719..3850)
                     /locus_tag="PLQ18_05225"
     CDS             complement(3719..3850)
                     /locus_tag="PLQ18_05225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE01089.1"
                     /translation="MKTTGTTAPAKATAAPVKPADVKNAKDAKNTKDAKQPTTTAQR"
BASE COUNT          546 a         1508 c         1452 g          511 t
ORIGIN      
        1 ggcggcacca agatcgaact gctcgcgctc gatgccgacg gccgcgaatg cctgcgccgg
       61 cgcgtgccga cgccgcaggg cgattacgcc gcgacgctgc aggcgatccg ggcgctggtc
      121 gaggcggccg agcaggagct gggcgggcgg gcgagcgtcg ggctcggcac gccgggttcg
      181 gcctcgccgc gggacgggcg catgcgcaac gccaattcga cctgcctgaa cggccggccg
      241 ctggcgcgtg atctcgaagc cctgctcaac cggccgctgc gcttcgccaa cgatgccaac
      301 tgtctggcgc tgtccgaagc cgtcgacggt gccgcggccg gggccggact ggtgttcgcg
      361 gtgatcctcg gcaccggtgt cggcggcggg ctggcggcgc acgggcgcgt gctcaacggc
      421 cacaacggca tcgccggcga atgggggcac aacccgctgc cgtggccggc gatcggcgag
      481 catccggggc cgctctgctg gtgcgggcgc agcggctgca tcgagacctg gctgtccggc
      541 cccggcttcg cgcgcgccca cgcccgcgag cacggcctgc agcagctcgt gccggccgag
      601 accatcgtcg cgcgcgcccg tgccggcgat ccggccgccg ccaccagcct agcgcgctac
      661 tgcgagcggc tggcgcgggc gctggcaacg gtggtgaacc tgctcgatcc ggaggtcatc
      721 gtgctcggcg gcggcctgtc gcgcgtcgac gagatctacg cgcgcgtgcc ggcgctgatg
      781 gcgccgcacg tgttcgccga tgccgtcgac acgccgctgc gcccggccct gcacggcgat
      841 tcgagcggcg tgcgcggcgc cgcctggctg tggaacgacg gcgccgccgt gagcgcttgc
      901 caagccggcc gcggcgacta gaacgtaagc ggacgcccgc cgcgggtggc cgccgcccgg
      961 acgccgcgcc ccgctgtccg ccccggcccg cacggaaggg acgcgaggta cggatcatgg
     1021 ccgacgaact cgacgcgggc cgcggcaagc tgctcgaaaa gctcaacgcc gccttcgagc
     1081 gcatgaccac gctcgacatc acgaccgcgg tcggcgaggc aacgaaaaac cccgccaccg
     1141 gccgctacga accggccgcc ggcagcaagc tgctgtacac gtcgatcgat ctggtcggcg
     1201 gcgacatcct gaacatcgtg ccggcggact tcggcgaagg tgccgacgat gcgctgcgcg
     1261 ccttccacca gtcgcaggtc gccagcgcgc aggcgacggt caaggccaac ctcgacgccg
     1321 tcgagaagct gatcgcgctc gcgaccggca aggactgaac gccgatggcc gatctgcgcc
     1381 agatcgcgct gtccatcgcg caggacctga tgacgctcga agtcaacacg gtcctcaagc
     1441 cgggcatgac cgcgcgcaag ccgccggcct atccgctgct gctgcgcgag atcgccgaga
     1501 agtacgtcgc cacgctcgtg caactcgacg ccggcccggc cccgatcccc ggcaatccga
     1561 ccgccgacac cgtgcacatc gacgccgtgc acgccgatga cgactcggtc gccgccgtgc
     1621 acatcgacgc cgtgctgcag gacgctgccg ccccggccga accgccggcc ccgccgccgc
     1681 cggcttccga gcacgacacc gcgatcggcc gggcgctggc gctgatcgcg cagacctttc
     1741 cgggcctggc cgcgccctac ggccacctcg ccgatgcgat caatgcgctg cacccgcccg
     1801 gcaacgccgc gaccttcgaa agcggggccg gaccgttcga tgagcgcttt gccggcgcgc
     1861 tgctgtgggc gacgctgttc ggcagttgcg ccgatgttgc cgcgacgctg ctcgcgcaga
     1921 ccccggccga atcgccggca cgcgcgccgc tgcggcgcat cgcacgcgaa tcgcgctggc
     1981 tgcaggcctt catcggctgg cagcacctgc agacgacgcc gggccgcggc ttcgacctgc
     2041 agcccgagga ccgcatgcgc ctgcgcaagc tctggaacct cggcggcgag cgcgtcgtga
     2101 tgcagacgac gatccacctc gacggcgacg tgctgaacgg catcgacccg gcgctgctgc
     2161 tgcatccgca gttcgaggcg gtcaaggcgc tgcatgccga tggcgtcggc accgcgctca
     2221 cgcactggcg cgcgctgttc gaggcgatct gcacggcctt cggcaagctc ggcgacgcgt
     2281 tctcgaatgt cctcgacaag cgccgcggct gagccgccgc gctgggtggc cggcctgctc
     2341 gattcgcgca gcggcgcccc ggccttctat ccgcgccccg gcctgcgcgc gctgctcgcc
     2401 gaactgctgc acggcgacgg cgcccagctc gtgaccgcgc gtaccggcgc cgacggcacg
     2461 ccggacctcg atcacgtcgc cgtgaccacg ctgctgcagc tcgacggcag cgtgctggtg
     2521 cagatcgacg aacgttcgct ggccgcgccc gactggccgg cggccttcgc cgtgcaccgc
     2581 gcggcactcg gcatcgccgg cgagcgcctg tccgcgtacg gcaaaagcgt ggcgacgacg
     2641 ctcacgtcga tccgcggcat tgccatgtgc acgctgcccg cactcgccag cccgaccccg
     2701 atgggcagcc gcgtggccgc gacggtctgc gcctggtggg ggttcagccc tgagacggca
     2761 ccgctgctcg cgaccggcct gcacgtgctg gcgcaggcgg tgatgccgat ggccgccggc
     2821 ttcgggatcg cccggctcgc cggcccgtgg ctggtccgcg cgctgctgcg cctcggcatg
     2881 tggtgggtcc ggcgctgacg gacgggcggc aggctcactc gccttccgcc cgcagccggt
     2941 agcgcggcag atgccagtcg cggtagatcg ccgcgagccg cagcgcgaac accgcggcca
     3001 tggcgacgag tgcggccgcg gccgccggca ggccgaggcc ggtcagcagc acgaacagca
     3061 ggccgccggc tgccgccgcg gtcgcgtaga tctcctgacg cagcaccagc ggcacctcgg
     3121 cgcagagcac gtcgcggatc gcaccgccgg cggcaccggt ggtgatgccg agcagcaccg
     3181 cgatcaccgc gccctggccc tgcgtgagcg cgacctgcgt gccgaccacc gtgaacagcg
     3241 acagccccgc ggcatcggcc accagcagcg cgcgcttcgg caccgggcgc tggcgccccc
     3301 acacccaggt cgcggcgacc gcgagcgcga tggtgagcag gtagtgggcg tcgctgaacc
     3361 agaccagcgg atggcgggcg agcagcaggt cacgcagtgt gccgccgccg accgcggtga
     3421 ccgcggccag caccagcgca ccgagccagt cgagctgctt gcgcgcggcg accagcgcgc
     3481 cgctgaccgc aaagacgaac acaccgaaca ggtcgaaggc gtagagcacg ggatcaccac
     3541 ggaagattga ggtcggggtc gggacccgaa ccgggcgcgc acggtagcgg ctgcctgcgc
     3601 ggcgcgcgag cttcggcgac ggccggccgt cagccgcacg ctacagcgtg cgtcagaaac
     3661 gccggacccc gcagcggcgc acgggcggct gcggggtccg gggatgtgac ggcagagatc
     3721 agcgctgcgc ggtcgtcgtc ggctgcttcg cgtccttcgt gttttttgca tcctttgcat
     3781 tcttcacatc ggcgggcttc accggggcgg ccgtcgcctt cgccggagcc gtggtgccgg
     3841 tggtcttcac cggcgcgacc ttcgctggcg tggccttcac cggcgtgacc ttcgccggcg
     3901 gcgtggtccg ggaggcgacc ggcctggccg gggcggcccg ggcgatgacg accggcttcg
     3961 cggccggtgc cgggggcttg ccggcgacgg tcgccggctt tgccgcggcc ttgcctg
//
LOCUS       DAOQJH010000174         3978 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_13887_length_3978_cov_3.468519, whole genome shotgun sequence.
ACCESSION   DAOQJH010000174 DAOQJH010000000
VERSION     DAOQJH010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3978
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13887_length_3978_cov_3.468519"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1464
                     /locus_tag="PLQ18_05230"
     CDS             <1..1464
                     /locus_tag="PLQ18_05230"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNE01090.1"
                     /translation="GKTADALAVIDGPMNEGLRSWRAALEDNLRLQETANRQVYEEAQ
                     DEYRSTQAWLIGIAALVLVLGIATAVLVTRSIVLPLREAVGVLGAVAGGDLSKHAPVA
                     GNDELTVLATALNQAIDAQRSALADIDAANREAQAAAAAQAEQERRLAAAEHEKREAE
                     RAQAEHERIEAARMQAEVAQIRAAVHEAAHGDLRQQLDIPADRPTGEVADALNRLFAD
                     LRLSITPIGENAHTLAAASEELGAVSKQMNTDAATNAEDAGRAALSAGQVSQRVENVA
                     AATEEMSASIREISRATAEASNVTDEAVRVATDAQALIQHLGESSSHIGEVVKLISSI
                     AEQTNLLALNATIEAARAGEAGKGFAVVAGEVKELAKATAGATDEIAQRVTGIQTDTQ
                     GAIASIGRIFEIIRRVSDIQTSVAGAVEEQNAVANDIAGNLAHTVQASGEIARGIDRV
                     AQTASSTEQGSREIQQAAGQLAKMAAQLQELVGRFRC"
     gene            complement(1574..2596)
                     /locus_tag="PLQ18_05235"
     CDS             complement(1574..2596)
                     /locus_tag="PLQ18_05235"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE01091.1"
                     /translation="MSTTPHLVPVPVSGTRPQPAGSKLPTVLFVDDEPPITRSLSALF
                     RRTGQYAVLTANSARDALELLGTHDIDVIVSDQRMPGMTGVELLREFATRSPRTMRIL
                     LTAWSDLSAITASINEGEVFRFVQKPWGNDLLATVAVAASASLAPANEPVHGAPDLVP
                     AAAQGTGVGILVVDDGRELLDLVRADFGSTHPVHVARHADQAIALLEQHEGIGIVVSD
                     VRVADEDMTDVIKGIKATHPAVLSVMVTALHDGPTVIDLINEGQLFRLIGKPVKPSVL
                     RLALGSALRKHEFLRNDPGALARHRPAEAPAVAERLQRRGLLKRLLGSVRHLFSFGFG
                     TRSAAS"
     gene            complement(2607..>3978)
                     /locus_tag="PLQ18_05240"
     CDS             complement(2607..>3978)
                     /locus_tag="PLQ18_05240"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNE01092.1"
                     /translation="LSGAVAVVLPSRALAAAIGAGAEDGNFALYNADHDYLVAGGPGG
                     EAGRAAGWIRQQRAAPDTIYSETMPLGLAGAARGWLVWYGRPDSDWATRPDVLGADRF
                     HRLAQAVVIGLAVVAALALALGARRRRVLERLVAERTRELHESQAALVQAGRLEGLGT
                     LAAGIAHEIKNPLAWTRSNLDVCVDHARTLASLSKDWREAQQPGTDTDARALKLGRTL
                     IAAETFVREETATELMQLLEDCADGTTRIQAIVEAVGSFSRLDQRQVASTEIPKLVAS
                     VLSIAGSEIRKCGARIDTRYAEDLPAIICAPGQLAQVLLNLVVNACHAMQGAAEHRLT
                     ISAELHARGRRVRLVVSDTGCGIPAEIRDHIFDPFFTTKDVGKGTGLGLHLAHQIVRA
                     HGGRILVDSTPGHGASFTLDLPVDASAELAAAAPVDAAATTAAAPRPAGNPDPSEPLR
                     PLGAAA"
BASE COUNT          626 a         1348 c         1498 g          506 t
ORIGIN      
        1 ggcaagaccg ccgacgcgct cgccgtcatc gacggaccga tgaacgaagg cctgcgcagc
       61 tggcgcgcgg cgctcgagga caacctgcgc ctgcaggaaa ccgccaaccg ccaggtctac
      121 gaagaagccc aggacgaata ccgcagcacg caggcgtggc tgatcggtat cgccgcgctg
      181 gtgctggtgc tcggcatcgc caccgcggta ctggtcacgc gcagcatcgt gctgccgctg
      241 cgcgaggcgg tcggcgtgct cggtgccgtc gccggcgggg atctttcgaa gcacgcgccg
      301 gtggccggca acgacgaact gacggtgctc gcgacggcgc tgaaccaggc gatcgacgcc
      361 cagcgcagcg cactcgccga catcgatgcc gccaaccgcg aagcccaggc cgctgctgcc
      421 gcgcaggccg agcaggaacg ccggctggcc gcggccgaac acgagaagcg cgaagccgag
      481 cgtgcgcagg cagagcatga acgcatcgag gccgcccgca tgcaggccga ggttgcgcag
      541 atccgtgcgg ccgtccacga agcggcgcac ggcgacctgc gccagcagct cgacatcccg
      601 gccgaccggc cgaccggcga ggtcgcggat gccttgaacc ggctgttcgc agacctgcgc
      661 ctcagcatca cgccgatcgg cgagaacgcg cacacgctgg ccgcggccag cgaggaactc
      721 ggggcggtca gcaagcagat gaacaccgat gccgccacca acgccgagga tgccggccgt
      781 gccgcgctca gcgccggtca ggtcagccag cgggtcgaga acgtcgcggc ggcgaccgag
      841 gagatgagcg cgagcatccg cgaaatctcg cgtgcgacgg ccgaggcttc gaacgtcacc
      901 gacgaggccg tgcgcgtggc caccgacgcg caggcgctga tccagcacct cggcgagagc
      961 agcagccaca tcggcgaggt cgtgaagctg atcagctcga tcgccgagca gaccaacctg
     1021 ctggcgctca acgcaaccat cgaggccgcg cgggccggcg aggccggcaa gggctttgcg
     1081 gtcgtggccg gcgaggtcaa ggagctggcg aaggcgacgg ccggggccac cgacgagatc
     1141 gcgcagcgcg tcaccggcat ccagaccgac acccagggcg cgatcgcgtc gatcggccgc
     1201 atcttcgaga tcatccgccg cgtcagcgac atccagacca gcgtcgccgg cgcggtcgag
     1261 gaacagaacg ccgtcgccaa cgacatcgcc ggcaacctcg cgcacacggt gcaggccagc
     1321 ggtgaaatcg cccggggcat cgaccgcgtc gcccagactg cgtccagcac cgagcagggc
     1381 agccgcgaga tccagcaggc cgccggccag ctcgcgaaga tggccgcgca gctgcaggag
     1441 ctggtcgggc gcttccgctg ctgagccgga ggccgtccag ggacgcgcag cgcgttttcc
     1501 ggcaggcaga aagccatcga ccccgtccgt ccggacgggg tcgatggctt tcagggcagc
     1561 ggatgccgcg gcttcaggag gcggccgagc gggtgccgaa gccgaacgag aacaggtggc
     1621 gcacgctgcc gagcaggcgc ttcagcaggc cgcggcgctg caggcgttcg gcgacggccg
     1681 gagcctcggc ggggcggtgg cgggcgaggg caccggggtc gttgcgcagg aactcgtgct
     1741 tgcgcagcgc cgagccgagg gcgaggcgca gcaccgaagg cttgaccggc ttgccgatca
     1801 gccggaacag ctggccttcg ttgatcaggt cgatgacggt cggaccgtca tgcagcgccg
     1861 tgaccatcac cgacagcacc gccgggtgcg tggccttgat gcccttgatc acatcggtca
     1921 tgtcctcgtc ggcgacgcgc acgtcggaga cgacgatgcc gatgccctcg tgctgttcga
     1981 gcagggcgat ggcctgatcg gcgtggcggg cgacatgtac cggatgcgtg ctgccgaagt
     2041 cggcccggac cagatcgagc agttcgcgac catcgtcgac gaccaggatg ccgacgccgg
     2101 tgccctgggc ggcggccggc accagatcgg gagcgccgtg caccggttcg ttggccgggg
     2161 ccagcgaggc ggaggcggca acggcgacgg tcgcgagcag gtcgttgccc cagggcttct
     2221 gcacgaagcg gaacacttcg ccttcgttga tgctggcagt gatcgccgag aggtccgacc
     2281 acgcggtcag caggatgcgc atcgtgcgcg gcgagcgcgt cgcgaactcg cgcagcagct
     2341 cgacaccggt catgcccggc atgcgctggt cgctgacgat cacgtcgatg tcgtgcgtgc
     2401 cgagcagctc cagcgcgtcc cgggcggagt tggcggtcag caccgcgtac tggccggtgc
     2461 gccggaacag cgccgacagc gaacgcgtga tcggtggttc gtcatcgacg aacagcacgg
     2521 tcggcagctt gctgccggcg ggctgcgggc gggtgccgga aacgggaaca ggaacgagat
     2581 gcggcgtcgt gctcatcggg ggtccctcag gcagccgcgc ccagcgggcg cagaggttcg
     2641 gacgggtccg gattgccggc cgggcggggg gcggcagcgg tggtggccgc agcatcgacc
     2701 ggagcggccg cggcaagttc ggcggaggca tcgaccggca gatcgagcgt gaagctcgcg
     2761 ccgtgccccg gcgtgctgtc gacgaggatg cgtccgccgt gggcacgcac gatctggtgc
     2821 gcgaggtgca ggccgaggcc ggtgcccttg ccgacgtcct tggtcgtgaa gaacggatcg
     2881 aagatgtggt cgcggatctc ggccgggatg ccgcagccgg tgtcactgac caccagccgc
     2941 acgcgccggc cccgcgcgtg cagctcggcg ctgatcgtca gccggtgctc ggcggcgccc
     3001 tgcatggcgt ggcaggcgtt gacgaccaga ttcagcagca cctgcgcgag ctggccgggc
     3061 gcgcagatga tcgccggcag gtcttcggcg tagcgggtgt cgatccgggc gccgcacttg
     3121 cggatttccg agccggcgat gctgagcacg ctcgcgacca gcttcgggat ctcggtgctg
     3181 gcgacctgac gctggtcgag ccggctgaag ctgccgacgg cctcgacgat ggcctggatg
     3241 cgcgtggtgc cgtcggcgca gtcttcgagc agctgcatca gctcggtcgc cgtttcctcg
     3301 cgtacgaagg tttcggcggc gatcagcgtg cgcccgagtt tcagcgcgcg cgcatcggtg
     3361 tcggtcccgg gctgctgcgc ctcgcgccag tccttgctca ggctggccag cgtgcgggcg
     3421 tgatcgacgc agacatcgag gttgctgcgc gtccaggcca gcgggttctt gatctcgtgg
     3481 gcgatgccgg ccgcgagcgt gccgaggcct tcgaggcgac cggcctgcac cagcgccgcc
     3541 tggctctcgt gcagttcgcg cgtgcgttcg gcgaccaggc gttcgagcac gcgccggcga
     3601 cgggcaccga gcgcgagcgc gagcgccgcg accacggcga ggccgatcac gaccgcctgc
     3661 gccagccggt ggaagcgatc ggcaccgagc acgtcggggc gcgtggccca gtcgctgtcc
     3721 gggcggccgt accagaccag ccagccgcgc gccgcgccgg cgaggccgag cggcatggtt
     3781 tcggagtaga tcgtgtcggg ggcggcgcgc tgctggcgga tccagccggc agcacggccg
     3841 gcctcgccgc cgggaccgcc ggcgaccaga taatcatgat cggcgttgta gagcgcgaag
     3901 ttgccgtcct cggcaccggc gccgatggcg gcggccagcg cgcgggacgg cagcacgacg
     3961 gcgaccgcgc cgctgagc
//
LOCUS       DAOQJH010000175         3965 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_13953_length_3965_cov_3.931969, whole genome shotgun sequence.
ACCESSION   DAOQJH010000175 DAOQJH010000000
VERSION     DAOQJH010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3965
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13953_length_3965_cov_3.931969"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            60..1358
                     /gene="guaD"
                     /locus_tag="PLQ18_05245"
     CDS             60..1358
                     /gene="guaD"
                     /locus_tag="PLQ18_05245"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004552699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008892 - guanine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006147 - guanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HNE01093.1"
                     /translation="MTELRAFRAELLDFTGDPGENPDSAHVRHRPDGLLLVADGRIVE
                     CGPAARLLPQLSPEVPVEYFPDRLLMPGFVDAHIHYPQTDIIASYGSDLLDWLQTYTY
                     PAERAFADPAHAHELAGVFIEELLRHGTTTALVMASVHPQSVDALFTAAAGQRLRLVA
                     GKLLMDRHVPEDLCDPADAGYADSLALIRRWHGHGRFHYAITPRFAPTSTEGQLDVCG
                     RLARLDDSLFIHSHVAESQRELEWVAGLFPWARSYLDVYDRFGLLRRRAVYAHCLHLD
                     AADRTRMCESGASVAFCPGSNLFLGSGLLDLPACAAGHLALGTDVGGGTSFSLLHTLA
                     EGYKVARLAGHTLAPLRGFYLATLGGARALDLDAHIGSFEPGREADFVVLDPGATQLL
                     TRRTARAVSLAERLFALMMLGDDRAIARVYVLGECVHAIG"
     gene            1578..1859
                     /locus_tag="PLQ18_05250"
     CDS             1578..1859
                     /locus_tag="PLQ18_05250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE01094.1"
                     /translation="MIRNDAWLADLRARLDDWSGRIEQLDQQARHADPTRRARLEEQL
                     VILRRLREDTENRLQTLLRATGTEPPPVLRARSSGAWQRRHQAFERLHG"
     gene            1974..2408
                     /locus_tag="PLQ18_05255"
     CDS             1974..2408
                     /locus_tag="PLQ18_05255"
                     /EC_number="1.11.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006948756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0006972 - hyperosmotic response [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0033194 - response to hydroperoxide
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HNE01095.1"
                     /translation="MKIRNSGSAVWSGGLKDGKGAISTQSGALDAHPYGFAMRFEGVP
                     GTNPEELIGAAHAGCFTMALSKILGEAGFTAERMETTAVVTLEQVGEGFSITGVALTL
                     RATVPELEAAQFQELAAKAKAGCPVSKLLKAEITLDAQLLTA"
     gene            complement(2641..>3965)
                     /locus_tag="PLQ18_05260"
     CDS             complement(2641..>3965)
                     /locus_tag="PLQ18_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005981195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNE01096.1"
                     /translation="IAFMLMHGEARAVLVDREFGDVIRRALQRVDHAVLVIDCDDPEY
                     AGPGERVGEVEYEALLAEGDPSYASDAPADEWDAIALNYTSGTTGNPKGVVTHHRGAY
                     LNAANNVIAWSLPHHPVYLWTLPMFHCNGWCFPWTLAAAAGVSVSLRAVRPEPIFKAI
                     KEEKVNHFCGAPIVLNMLNNAPAALKDGITHQVKVMTAGAAPPAAVIEGMERMGFQVT
                     HVYGLTETYGPCVSCAWQDRWNDESIEEKARLKARQGVRYPMQEGLMVADPTNLEPVP
                     QDGKTMGEVFMRGNLVMKGYLKNPSATEESFQGGWFHSGDLAVWHEDGYIEIKDRSKD
                     IVISGGENISTIEVEDVLYRHPAVMEAAVVARPDSKWGETPCAYVTLKPEKEGEVQES
                     EIIAFCKTQMAGYKVPKTVIFGPLPKTSTGKIQKFVLRNQAKELGSLS"
BASE COUNT          574 a         1401 c         1344 g          646 t
ORIGIN      
        1 agcacctaag cttctccggc gtgccgcagc cggcggcgct gaagccctgc ggtgccgcca
       61 tgaccgagct gcgtgcgttt cgtgccgaac tgctggactt caccggcgac ccgggtgaga
      121 accccgattc ggcgcatgtg cgccaccggc ccgacggcct gctgctggtt gccgatggcc
      181 gcatcgtcga gtgcgggccg gccgcgcggc tgctgccgca gctgtcgccc gaagtgccgg
      241 tcgagtactt cccggaccgg ctgctgatgc cgggcttcgt cgatgcgcac atccattacc
      301 cgcagaccga catcatcgcc agctacggca gcgacctgct cgactggctg cagacctaca
      361 cctatccggc cgaacgcgcc ttcgccgatc cggcgcacgc gcacgagctc gccggggtgt
      421 tcatcgagga actgctgcgc cacggcacga ccacggcgct ggtgatggcc agcgtgcatc
      481 cgcagtcggt cgatgcgctg ttcacggccg cggccggaca gcggctgcgg ctggtcgccg
      541 gcaagctgct gatggatcgc cacgtgccgg aggatctctg cgacccggcc gacgccgggt
      601 acgccgactc gctggcgctg atccggcgct ggcacggcca cgggcgcttc cactacgcga
      661 tcacgccgcg cttcgcgccg acctcgaccg agggccagct cgatgtctgc gggcggctcg
      721 cgcgcctcga cgacagcctg ttcatccatt cgcatgtcgc cgagagccag cgcgagctcg
      781 aatgggtcgc tggcctgttc ccgtgggcgc gcagttatct cgatgtctat gaccgcttcg
      841 gcctgctgcg ccggcgcgcc gtctacgcgc actgcctgca cctcgatgcc gccgatcgca
      901 cgcgcatgtg cgaatccggc gccagcgtcg cgttctgccc gggctcgaac ctgttcctcg
      961 gcagcggcct gcttgatctg ccggcctgtg ccgccgggca tctggccctc ggcaccgatg
     1021 tcggcggcgg cacgagcttc tcgctgctgc acacgctggc cgagggctac aaggtggcgc
     1081 ggctggccgg acacacgctg gcgccgctgc gcgggttcta tctcgcgacc ctcggcggcg
     1141 cccgcgcact cgatctcgat gcgcacatcg gcagcttcga acccggacgc gaggccgact
     1201 tcgtcgtgct cgatccgggc gcgacgcagc tgctgacgcg gcgcaccgcc cgtgccgtgt
     1261 cgctcgccga acggctgttc gcgctgatga tgctcggcga cgaccgtgcc atcgcccggg
     1321 tctacgtgct cggcgagtgc gtgcacgcga tcggctgagg cgcgccgcag ggcatcgccg
     1381 cgtatggctg cagtgcgtgc agccatcgcg tcaaggaagc attacagaac attccccttg
     1441 aaaatcatgt tcatgccttg aatcacccgc ttcaccggca gcggttcgca ttgctttgtg
     1501 cgcccgcgca gcgaaccact gccgcacacc cggcgtcgga acgcatcgtg ttcgacagaa
     1561 aaccggagaa cgaaccgatg atccgcaacg atgcctggct cgccgacctg cgtgcgcgcc
     1621 tcgatgactg gagcggcagg atcgagcagc tcgaccagca ggcccgtcac gccgatccga
     1681 cgcgccgcgc gcggctcgag gaacagctcg tgatcctgcg ccggctgcgc gaggacaccg
     1741 aaaaccgcct gcagacgctg ctgcgcgcca ccggcaccga gcctccgccc gtgctgcgcg
     1801 cgcgcagcag cggtgcctgg cagcgccggc atcaggcttt cgagcgcctg catggctgac
     1861 accgcctgca ggccggcatc ctgctgatcc gcccgctttc cgccgccggc cgcccggccg
     1921 gcagcacctc atccggcagc gccggatgcc atcgaacgag gagacatgat ccgatgaaga
     1981 tccggaacag cggttcggcc gtctggagcg gcggcctgaa ggacggcaag ggcgcgatct
     2041 cgacgcagag cggtgcgctc gatgcgcacc cttacggctt cgcgatgcgc ttcgagggcg
     2101 tgcccggcac caatcccgag gagctgatcg gcgcggcgca cgccggctgc ttcacgatgg
     2161 cgctgtcgaa gatcctcggc gaggccggct tcacggccga gcgcatggaa acgacggccg
     2221 tggtcacgct cgaacaggtc ggcgagggct tttcgatcac cggcgtcgcg ctgacgctgc
     2281 gcgcaacggt gccggagctg gaggcggcgc agttccagga actcgccgcc aaggccaagg
     2341 ccggctgccc ggtctcgaag ctgctgaagg ccgagatcac gctcgatgcg cagctgctga
     2401 cggcctgagc gcgggccgga aggcgggcgg gccgggaatc tcccctcccc cgatcccctg
     2461 ccagcgaacg gcgaaggcct ggaaaccccg cgcctgccgg gaccggcctt ctgcagatcc
     2521 gcagcggcac aggcgccatg aatgacgacg ccccgcgaat gcggggcgtc cttgtgcagg
     2581 cgatccactg caccggcagc atggcggtct tgcagccggt gcgtgccggc cgctgcaggc
     2641 tcagctcagc gagccgagtt ccttcgcctg gttgcgcagc acgaacttct ggatcttgcc
     2701 ggtcgaggtc ttcggcagcg ggccgaagat gacggtcttc ggcaccttgt agccggccat
     2761 ctgcgtcttg cagaaggcga tgatctcgga ctcctgcacc tcgccttcct tctccggctt
     2821 cagcgtcacg taggcacacg gggtctcgcc ccacttgctg tccgggcggg cgacgaccgc
     2881 ggcttccatg accgccggat ggcggtagag cacgtcctcg acctcgatcg tcgagatgtt
     2941 ctcgccgccg gagatgacga tgtccttcga gcggtccttg atctcgatgt agccgtcctc
     3001 gtgccagacg gcgaggtcac cggaatggaa ccatccgccc tggaaggatt cctcggtggc
     3061 gctcgggttc ttcaggtagc ccttcatgac caggttgccg cgcatgaaca cctcgcccat
     3121 ggtcttgccg tcctgcggca ccggttcgag gttggtcgga tcggcgacca tcaggccttc
     3181 ctgcatcgga tagcgcacgc cctgacgggc cttgaggcgc gccttctcct cgatcgactc
     3241 gtcgttccag cgatcctgcc aggcgcagga cacgcagggg ccgtaggtct cggtcaggcc
     3301 gtagacgtgc gtgacctgga agcccatgcg ctccatgccc tcgatgacgg cggccggcgg
     3361 cgcggcgccg gcggtcatga ccttgacctg atgcgtgatg ccgtccttca gcgccgccgg
     3421 ggcgttgttc agcatgttca gcacgatcgg cgcgccgcag aagtgattga ccttctcttc
     3481 cttgatcgcc ttgaagatcg gctcggggcg cacggcgcgc aggctcaccg acacgccggc
     3541 ggccgcggcc agcgtccacg ggaagcacca gccgttgcag tggaacatcg gcagcgtcca
     3601 caggtacacg gggtggtgcg gcaggctcca cgcgatgacg ttgttggccg cgttgaggta
     3661 ggcgccgcgg tggtgggtga ccacgccctt ggggttgccg gtggtgcccg aggtgtagtt
     3721 gagcgcgatc gcgtcccact cgtcggcggg cgcgtcgctc gcgtacgagg ggtcgccctc
     3781 ggcgagcagg gcctcgtact ccacctcgcc gacgcgctcg ccgggccccg cgtactccgg
     3841 gtcgtcgcag tcgatcacca gcaccgcgtg gtcgacgcgc tggagggcgc ggcggatgac
     3901 gtcgccgaac tcccggtcga cgaggacggc ccgcgcctcg ccgtgcatca gcatgaaggc
     3961 gatgg
//
LOCUS       DAOQJH010000176         3951 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_14035_length_3951_cov_4.068275, whole genome shotgun sequence.
ACCESSION   DAOQJH010000176 DAOQJH010000000
VERSION     DAOQJH010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3951
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14035_length_3951_cov_4.068275"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1345)
                     /locus_tag="PLQ18_05265"
     CDS             complement(<1..1345)
                     /locus_tag="PLQ18_05265"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNE01097.1"
                     /translation="MSRWQAGMPVRLLDDPGQTGVVTGLTRERGEVQWVQVRFPAGCQ
                     YYADYELETLDAAPADPYDRVLAGRFGRTEDLRRQLTRIQLSGRLANLIYSLDTTATD
                     FYAHQFKPVLSFLESPCNGLLIADEVGLGKTIEAGLIWTELRARLDARRLLVVCPAML
                     REKWRDELRRRFGIRAELVGAGELLAALREPKDTWPAERALVGSLQGLRPSGDWQSTD
                     EQGERAQLARLLDEQADAEPLFDLVVIDEAHYLRNPQSLSAELGRLLREVSAHLVLLS
                     ATPVNTRADDLFELLRLVDPENFRYGEQFQQVLLANRPLLRARACALDLRASLAQVVD
                     ELRKAQAHPLLAGSRQLQDLIAACTDGPAPDTRATRIELANRIERVNLLAHAVTRTRK
                     AEVQERRVVRRVRVEAVEPTDAERALYESVTDAVRGYAWQRDVSDGFLLAMPQRQV"
     gene            1769..3553
                     /locus_tag="PLQ18_05270"
     CDS             1769..3553
                     /locus_tag="PLQ18_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010221289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNE01098.1"
                     /translation="MPSLRRPPSAFHRARRAAPATALALGIALSLTFPVAADEVSRPP
                     AAPDPLYDPKPLADDIELPLPCDGRLLLRTVYVLAKSALDDREVNLGYPFTQDEPGYK
                     QGFVAGYRLGHVNGQFEPADLPTAWRTRLDGKLPAAGEGGLKPMLYFIGKYEITQRQY
                     RLVMALEPWLHGAAPEPPACPAPAATDRLPMVDLSWFDALNFTALYTEWLMRKHREAL
                     PALGGGAGSAAGGASAYVRLPTETEWEYAARGAQVVDRQALEARLFPRREGNASEDGP
                     LRDWAVYTQVAGGSGQTARLAPIGTKRPNPVGLFDVIGNAAEMVLDPFLLVRGGGRPG
                     GGTGGFVVKGGNYLEGEQTLFTGMRREYPLFDDKGQPSRNTTTGFRIALGTLAAPRAR
                     YDELFAGWQAEGRLSNLTSELPPDADAGVKLDRIVADLQRDMPTSTIGRELGTVSEEL
                     KRSVALIAAQRRDAAANLIQSSALVAETIGNYNIRLTNLNKQLEEQKAKRNAEGVQFF
                     TNVIADGTSAVQAALSIYLDNVNSGAAYAETLFAEQFQRVQDELGKKPVLGRSLSARA
                     TLFMKHVEQYRETRRLDRDRVLNEVLGR"
     gene            3568..>3951
                     /locus_tag="PLQ18_05275"
     CDS             3568..>3951
                     /locus_tag="PLQ18_05275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE01099.1"
                     /translation="MRFLLAPLAVATLAGCCVCAPSPAPVAAGDVPVAPGTPPLTDFY
                     ADCGRGLLDAARLELELERDAGRAAVWNGYIAATQRTQDCYWAAYQPLLAQHRSGSLN
                     RVEGRARLRELGDGIAATRVALARNL"
BASE COUNT          614 a         1412 c         1344 g          581 t
ORIGIN      
        1 atacctgacg ctgcggcatg gcgagcagga agccgtcgct cacgtcgcgt tgccacgcat
       61 agccgcgcac ggcatcggtc acgctttcgt agagcgcgcg ttccgcatcg gtcggttcga
      121 ccgcttcgac gcgcacgcgc cgcacgacgc gccgctcctg cacttcggcc ttgcgggtgc
      181 gggtcaccgc gtgcgcgagc aggttcacgc gctcgatgcg attggccagt tcgatgcgcg
      241 tcgcccgcgt atcgggtgca ggcccgtcgg tgcaggcggc gatcagatcc tgcaactggc
      301 ggctgccggc gagcagcgga tgcgcctgcg ccttgcgcag ctcgtcgacg acctgcgcga
      361 gactcgcccg caggtccagc gcgcacgcgc gcgcccgcag cagcggacga ttcgcgagca
      421 gcacctgctg gaactgctcg ccgtagcgga agttttcggg atcgaccagt cgcagcagct
      481 cgaacagatc gtcggcgcgc gtgttgaccg gggtggccga cagcagcacg agatgcgcgc
      541 tgacctcgcg cagcaggcgg ccgagttcgg cgctcagact ctgcggattg cgcaggtagt
      601 gcgcctcgtc gatgacgacc agatcgaaca ggggttcggc gtcggcctgt tcatcgagca
      661 ggcgtgcgag ttgtgcccgc tcgccctgct cgtcggtgct ttgccagtcg ccggagggcc
      721 gcagaccctg caggctgccg accagggcgc gctcggcggg ccaggtgtcc ttcggctcgc
      781 gcagcgcggc gagcaattca ccggcaccga ccagttcggc gcggatgccg aagcgccggc
      841 gcagttcgtc ccgccatttt tcacgcagca tcgccgggca gacgacgagc agccggcgtg
      901 cgtcgaggcg tgcgcgcagc tcggtccaga tcaggccggc ttcgatggtc ttgccgagac
      961 cgacctcatc ggcgatcagc aggccgttgc acggtgattc caggaacgag agcaccggct
     1021 tgaactggtg cgcgtagaag tcggtggcgg tggtatcgag gctgtagatc aggttcgcga
     1081 gccggcccga gagctggatg cgcgtcagct gccggcgcag atcctcagtg cggccgaagc
     1141 gaccggcgag cacgcgatcg tacggatcgg ccggcgcggc atcgagggtt tccagttcgt
     1201 aatcggcata gtactgacag ccggccggaa accggacctg tacccactgc acctcgccgc
     1261 gctcacgggt gagtccggtg acgacgccgg tctgaccggg atcgtcgagc agacgcaccg
     1321 gcatgcccgc ctgccagcgg ctcatgatcg ggcctcctgc tgcgcatggc ggacagggcc
     1381 gcaaaccggc tgccggcgat cggggtagtg cgccgctgct ggtcgtgata gggccatgct
     1441 ggctttgcat ccctcgatgc acatccggtc gactccctga gcagatgtcc ggcaatccgg
     1501 ctcatgaaaa cgcgattatt cttacaagta tatgatcatg atcaattaat cggcgagcat
     1561 gagtctcgcc tgaccgcagc ccctgcggga gcggtgccgc agggtggctg caaatgcccg
     1621 atcagcggct ccggatgacg gctgcaggcc atgcagccat gatccgccgg aagcctgcac
     1681 catcacccga acacgcaccc ggatgcactg gcaggccgtg gcgcgctacg ctcgccggac
     1741 ttccgctgcc cgcccaggct taccgcagat gccgtcgctc cgtcgtccgc cgtccgcttt
     1801 ccaccgcgcg cgccgcgccg cgccggccac cgccctcgcc ctgggcatcg ccttgagtct
     1861 caccttcccg gttgccgccg atgaggtcag ccggccgccg gccgcccccg atccgctcta
     1921 cgacccgaag ccgctcgccg acgacatcga actgccgctg ccctgcgacg gccggctgct
     1981 gctgcgcacc gtctacgtgc tcgccaagag cgcgctcgat gaccgcgagg tcaacctcgg
     2041 ctatccgttc acgcaggacg aacccggcta caagcagggc ttcgtcgccg gttaccggct
     2101 cggccacgtc aacggccagt tcgaaccggc cgacctgccg acggcgtggc gcacgcgcct
     2161 cgacggcaag ctgccggcgg ccggtgaagg cgggctgaag ccgatgctgt acttcatcgg
     2221 caagtacgag atcacgcagc gccagtaccg gctggtgatg gcgctggagc cctggctgca
     2281 cggtgccgcg cccgagccgc cggcctgtcc ggcgccggcg gcgaccgacc ggctgccgat
     2341 ggtcgacctg agctggttcg acgcgctgaa cttcacggcg ctgtacaccg aatggctgat
     2401 gcgcaagcac cgcgaggcgc tgccggcgct cggcggcggc gccggttcgg cggccggcgg
     2461 ggcttcggcc tacgtgcgcc tgccgaccga gaccgaatgg gagtacgcgg cgcgcggtgc
     2521 gcaggtggtc gaccggcagg cgctcgaagc gcggctgttc ccgcgccgcg aaggcaatgc
     2581 cagcgaggac gggccgctgc gcgactgggc ggtatacacg caggtcgccg gcggcagcgg
     2641 ccagacggcg cggctcgcgc cgatcggcac gaagcgtccg aacccggtcg ggttattcga
     2701 cgtgatcggc aacgccgccg agatggtgct cgatcccttc ctgctggtgc gcggcggcgg
     2761 ccggcccggc ggcggcaccg gcggcttcgt ggtcaagggc ggcaactacc tcgaaggcga
     2821 gcagacgctg ttcaccggca tgcgccgcga gtacccgctg ttcgacgaca agggccagcc
     2881 gagccgcaac acgacgacgg gcttccgcat cgcgctcggc acgctggccg caccgcgcgc
     2941 gcgctatgac gaactgttcg ccggctggca ggccgagggg cgtctgagca acctgaccag
     3001 cgagctgccg cccgacgccg atgccggcgt caagctcgac cgcatcgtcg ccgacctgca
     3061 gcgcgacatg ccgacctcga ccatcggccg cgagctcggc acggtcagcg aggaactcaa
     3121 gcgctcggtg gcactgatcg ccgcgcagcg gcgcgatgcc gcggccaacc tgatccagtc
     3181 ctcggcgctg gtcgccgaaa ccatcggcaa ctacaacatc cgcctgacca acctgaacaa
     3241 gcagctcgaa gagcagaagg cgaagcgcaa cgccgagggc gtgcagttct tcaccaatgt
     3301 cattgccgac ggcaccagtg cggtgcaggc ggcgctgtcg atctacctcg acaacgtcaa
     3361 ctccggcgcc gcctacgccg aaaccctgtt cgccgagcaa ttccagcgcg tgcaggacga
     3421 actcggcaag aagcccgtgc tcgggcgcag cctgagcgcg cgcgcgacgc tgttcatgaa
     3481 gcatgtcgag cagtaccgcg agacgcgccg gctcgatcgg gaccgcgtgc tgaacgaagt
     3541 gctcggacgt taggaatcct caccctcatg cgcttcctcc ttgcccccct cgccgtcgcc
     3601 acgctggccg gctgctgcgt ctgtgccccg tcaccggccc cggtcgcggc cggcgacgta
     3661 ccggtcgccc ccggcacgcc gccgctgacc gatttctacg ccgactgcgg ccgcggcctg
     3721 ctcgatgccg cgcgtctcga actcgaactc gaacgcgatg ccgggcgcgc ggcggtctgg
     3781 aacggctaca tcgccgccac gcagcgcacg caggactgct actgggccgc gtaccagccg
     3841 ctgcttgcgc agcaccgcag cggcagcctg aaccgcgtcg agggccgtgc gcgcctgcgc
     3901 gaactcggcg acggcattgc cgcgacccgc gtcgcgctcg cccgcaacct g
//
LOCUS       DAOQJH010000177         3946 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_14057_length_3946_cov_3.725777, whole genome shotgun sequence.
ACCESSION   DAOQJH010000177 DAOQJH010000000
VERSION     DAOQJH010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3946)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3946)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3946
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14057_length_3946_cov_3.725777"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1779)
                     /gene="rnr"
                     /locus_tag="PLQ18_05280"
     CDS             complement(<1..1779)
                     /gene="rnr"
                     /locus_tag="PLQ18_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018954220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HNE01100.1"
                     /translation="MTARSRRPWHRLDPFFAREQEKYGRAVPSREFILQTLEAAPEPL
                     TLERLCADWGIHAQDETEPLLRRLQAMTRDGQIVCNRRGGYGPVAKMDLVRGRIIGHP
                     EGFGFLVPDEGGEDLFVPPHEMRQLLHGDRVLMCVTGIDRRGRREGSVVEVLERGTTR
                     LVGRYVEDNGIGFVLPDNRRLGAHDFLVPPAEKGGARPGQIVVAELIQQPSKQAMPIA
                     RVREVLGDHMAPGMEVQIAIVNHGIPHEFPPEVVAEAEAFGPSVPASALEGRLDLREQ
                     PFVTIDGLTARDFDAVYCEKKRGGGFRLFVAIADVAHYVRPGTPLDAEAQARGTSVYF
                     PDRCIPMLPEALSNGLCSLNPQVERLVMVCELNIGADGEFGRARFHEGVIRSHARLTY
                     DAVAAMLVDRDEALRAEFVALVPHLERLYALYGVLRAARERRGAIDFETQETEIEYGP
                     ERKIERIVPSQRNDAHKLIEECMIAANVATAQHLEAKKIPALFRIHEGPRLAKLEKLR
                     GFLKGLGLYLGGGDTPGSEDYARLFEAIQNRPDFALIQTVMLRSLAQAVYSPDNAGHF
                     GLALAAYAHFTSPIRRYPDLLTHRVIK"
     gene            1959..2043
                     /locus_tag="PLQ18_05285"
     tRNA            1959..2043
                     /locus_tag="PLQ18_05285"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1993..1995,aa:Leu,seq:cag)
     gene            2060..2144
                     /locus_tag="PLQ18_05290"
     tRNA            2060..2144
                     /locus_tag="PLQ18_05290"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2094..2096,aa:Leu,seq:cag)
     gene            complement(2291..3079)
                     /locus_tag="PLQ18_05295"
     CDS             complement(2291..3079)
                     /locus_tag="PLQ18_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020307904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HNE01101.1"
                     /translation="MLLSALYRYPVKSCRGARLDAAMLDRYGIAGDRHWMLVEAASGR
                     FVTQRQHARMALIDTQWHGDTLGLSVAGAGGVEVTSGIGEPRTVEVWGEVVQALDAGD
                     EAAAWLSEYLGLAVRLVECGPAHRRPVDPAYDRIGAETGFSDGFPLLLISEASLADLN
                     SRMERPVEMIRFRPNLVVSGCAPFAEDGWKRLRIGGIELHVVKPCSRCPIPTIDPATA
                     ARGREPMATLLKYRREGQKTYFGQNLIHAGPGELQAGMRVEVLE"
     gene            complement(3161..>3946)
                     /locus_tag="PLQ18_05300"
     CDS             complement(3161..>3946)
                     /locus_tag="PLQ18_05300"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003461081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase family protein"
                     /protein_id="HNE01102.1"
                     /translation="VPVVVQLLGSDPQCLADNAARVAGLGAPGVDLNFGCPAPTVNRH
                     RGGAALLDEPELLARIVAACRAALPPAVPLTAKMRLGIRDAARALDCARALADGGAAE
                     ITVHARTKLDGYRPPAHWDWIARIREAVAVNVIANGEVWSPADAARCLAVSGCRDLML
                     GRGLVTRPGLALNIRSQRAGHEPLPLPWPRLLALLDTFYAGVRAQLLPRHAPGRLKQW
                     LSYLRNAYPEAQALFARVRTETDAARLGEALAAAHAHPVPLPP"
BASE COUNT          607 a         1361 c         1399 g          579 t
ORIGIN      
        1 cttgatgaca cggtgagtca gcaggtcggg atagcggcgg atcggcgacg tgaagtgcgc
       61 ataggccgcg agcgcgaggc cgaagtgacc ggcgttgtcg gggctgtaca cggcctgcgc
      121 cagcgagcgc agcatgacgg tctggatcag cgcgaagtcc ggccggttct ggatggcctc
      181 gaacagccgc gcgtaatcct ccgagcccgg cgtgtcgccg ccgccgagat acagcccgag
      241 ccccttcagg aagccgcgca gcttttcgag cttcgcaagc cgcggacctt cgtggatgcg
      301 gaacagcgcc gggatcttct tcgcttcgag gtgctgcgcg gtcgcgacat tggccgcgat
      361 catgcattcc tcgatcagct tgtgcgcatc gttgcgctgc gagggcacga tgcgctcgat
      421 cttgcgctcc ggcccgtatt cgatctcggt ttcctgggtt tcgaagtcga tcgccccgcg
      481 gcgctcgcgc gccgcgcgca gcacgccgta cagcgcgtac agacgttcga gatgcggcac
      541 gagggcgacg aactccgcgc gcaaggcctc gtcgcgatcg accagcatcg cggccaccgc
      601 gtcgtaggtc agccgagcgt gcgagcggat cacgccttcg tgaaagcgcg cgcgcccgaa
      661 ctcgccgtcg gcgccgatgt tcagctcgca gaccatcacc agccgctcga cctgcgggtt
      721 cagcgagcag agcccgttcg acagcgcctc gggcagcatc gggatgcagc ggtccgggaa
      781 gtacaccgag gtgccgcgcg cctgcgcctc ggcatcgagc ggcgtgccgg ggcgcacgta
      841 atgcgcaacg tcggcaatgg cgacgaacag ccggaagccg ccgccgcgct tcttctcgca
      901 gtagacggcg tcgaagtcgc gggcggtgag accgtcgatg gtcacgaacg gctgctcgcg
      961 caggtcgagc cggccttcga gcgccgatgc gggcacgctc gggccgaagg cttcggcctc
     1021 ggcgacgact tcgggcggga actcgtgcgg aatgccgtga ttgacgatgg cgatctgcac
     1081 ttccatgccc ggcgccatgt gatcgccgag cacctcgcgc acgcgcgcga tcggcatcgc
     1141 ctgcttcgag ggctgctgga tcagctcggc gacgacgatc tgcccggggc gcgcaccgcc
     1201 cttctcggcc ggcggcacca ggaagtcgtg cgcgccgagc cggcggttgt cgggcagcac
     1261 gaaaccgatg ccgttgtcct cgacgtagcg cccgaccagc cgcgtcgtgc cgcgttcgag
     1321 gacctcgacc accgagccct cgcgccggcc gcggcggtcg atgccggtca cgcacatcag
     1381 cacgcggtcg ccgtgcagca gctggcgcat ctcgtgcggc ggcacgaaca gatcctcgcc
     1441 gccctcgtcc ggcaccagga agccgaagcc ctcggggtga ccgatgatgc gcccgcgcac
     1501 cagatccatc ttcgcgaccg gcccgtagcc cccgcggcgg ttgcagacga tctggccgtc
     1561 gcgcgtcatc gcctgcaggc ggcgcagcag cggttcggtt tcgtcctggg cgtggatgcc
     1621 ccagtcggcg cacagccgtt cgagcgtcag cggctcgggc gcggcttcga gcgtctgcag
     1681 gatgaattcg cggctcggta cggcgcggcc gtatttttcc tgctcgcgcg cgaagaacgg
     1741 gtcgagccgg tgccagggac gtcgggaacg ggcagtcatc gggaatgcgg aaacctcgcg
     1801 aaaaatgaag gaatgacacg ataacaccgc cccgtccgga gcatgcacaa aacgtgcgga
     1861 gggcgtttga cagcccccgg ggccgtgcgt atagtgcgcc ccacttcgcg accccctccc
     1921 ctgcggaccg ggatcgtggc gtgaaatgca gcagccttgc cgaagtggcg gaactggtag
     1981 acgcgctagt ttcaggtact agtggggcga cccgtggagg ttcgagtcct ctcttcggca
     2041 ccacgcgctg cagcaagatg cccaggtggc ggaactggta gacgcgctag tttcaggtac
     2101 tagtagggag acctgtggag gttcgagtcc tctcctgggc accactccat catgatcatg
     2161 atgatgagtg tctctcccga gaaaacggcc ccgaaagggg ctttttcttt tccggcttcc
     2221 ggcgaacgtc cgcgtgctgt tccggcaccg cccgctggcc gctgcaacga ccagcggcgg
     2281 gcgcgcatgg ctattccagc acctccacgc gcatgccggc ctgcaattcc cccggaccgg
     2341 catggatgag gttctggccg aagtaggtct tctgcccttc gcggcggtac ttcagcagcg
     2401 ttgccatcgg ttcgcgcccg cgcgcggccg ttgccggatc gatggtcgga atcgggcagc
     2461 gcgagcacgg cttgaccacg tgcagctcga tgccaccgat gcgcagccgc ttccagccat
     2521 cctcggcgaa gggcgcgcag ccgctgacca ccagattcgg ccggaagcgg atcatctcga
     2581 ccgggcgctc catgcggctg ttgaggtcgg ccagcgaagc ctccgagatc agcagcagcg
     2641 ggaagccatc ggaaaagccg gtctcggcgc cgatgcgatc gtaggccgga tcgaccggcc
     2701 gccggtgcgc ggggccgcat tcgaccaggc gcacggcgag cccgaggtac tccgacagcc
     2761 aggccgcggc ctcgtcgccg gcatcgagcg cctgcacgac ctcgccccag acctcgaccg
     2821 tgcgcggctc gccgatgccg ctggtgacct cgacgccacc ggcaccggcc accgacaggc
     2881 cgagcgtgtc gccatgccac tgcgtgtcga tcagcgccat gcgcgcgtgc tggcgctgcg
     2941 tgacgaagcg gccgctcgcg gcctcgacca gcatccagtg ccggtcgccg gcgatgccgt
     3001 agcgatcgag catcgcggca tcgaggcgcg cgccgcggca ggacttcacg gggtagcggt
     3061 acagcgcgct cagcagcacg tcggtcatcg ggcagcctgc ctgtgggtgg tccgggaaaa
     3121 acatccggcg cgactgtagc gaatgccgcc ggcacgcgcc tcagggcggg agcggtacgg
     3181 gatgggcgtg cgcggcggcc agcgcctcgc caagtcttgc ggcatcggtt tcggtgcgca
     3241 cgcgcgcaaa cagcgcctgt gcttccggat aggcgttgcg cagataggac agccactgct
     3301 tcagccgccc cggcgcgtgc cgcggcagca gctgcgcacg cacgccggca tagaaggtat
     3361 cgagcagcgc cagcagccgc ggccagggca gcggcagcgg ttcgtggccg gcgcgctggc
     3421 tgcggatgtt cagcgccagc cccgggcgcg tcaccagccc gcgcccgagc atcaggtcgc
     3481 ggcagccgga aacggcgagg caccgggcgg catcggccgg gctccagacc tcgccgttgg
     3541 cgatgacgtt gacggcgacc gcctcgcgga tgcgggcgat ccagtcccag tgcgccggcg
     3601 gccggtagcc gtcgagcttc gtgcgggcat gcacggtgat ttcggccgcc ccgccgtcgg
     3661 cgagcgcccg tgcgcagtcg agggcgcgcg cggcatcgcg gatgccgagg cgcatcttgg
     3721 cggtcagcgg caccgccggc ggcagcgccg cgcggcaggc ggcgacgatg cgcgcgagca
     3781 gttccggctc atcgagcagc gccgcgccgc cgcggtgacg gttcacggtc ggggccgggc
     3841 agccgaagtt cagatccacg cccggcgcgc cgagcccggc gacgcgcgcg gcgttgtcgg
     3901 cgaggcactg cggatcggag ccgagcagct gcacgaccac cggcac
//
LOCUS       DAOQJH010000178         3945 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_14062_length_3945_cov_3.382519, whole genome shotgun sequence.
ACCESSION   DAOQJH010000178 DAOQJH010000000
VERSION     DAOQJH010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3945)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3945)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3945
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14062_length_3945_cov_3.382519"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..483)
                     /locus_tag="PLQ18_05305"
     CDS             complement(<1..483)
                     /locus_tag="PLQ18_05305"
                     /inference="COORDINATES: protein motif:HMM:NF024750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
                     /protein_id="HNE01103.1"
                     /translation="MKRLTDLELRRLAKRAAEGLESPREVREGEGFGVSVLPSGKLSF
                     FLLYTLGGVRRRIRFGTYPETTLAAARDAVAAVRAQLAEGLDPAAERVRKRNAVAEAV
                     AVDRRRPTVRALAGEYTDRHLPMLAESTRKRALLELKEIVRAWGPRKADEITRRDVVL
                     L"
     gene            complement(790..1050)
                     /locus_tag="PLQ18_05310"
     CDS             complement(790..1050)
                     /locus_tag="PLQ18_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018952846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HNE01104.1"
                     /translation="MDCYELVWKRSAERELRKLPREVIAALVLLAEGLVCDPYPPGAR
                     KLAGARHTWRVRSGDYRLIYTVEDARLVVQVIRVGHRREVYR"
     gene            complement(1037..1240)
                     /locus_tag="PLQ18_05315"
     CDS             complement(1037..1240)
                     /locus_tag="PLQ18_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE01105.1"
                     /translation="MATVFESLHPQFITDADGARVAVVLSLAEYEALLEDIADLAAVA
                     ERQDEDCVSFEELKAGLRADGLL"
     gene            1621..>3945
                     /locus_tag="PLQ18_05320"
     CDS             1621..>3945
                     /locus_tag="PLQ18_05320"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HNE01106.1"
                     /translation="MPAKPLHPDVVRRNADKVAEQMGRADQERFERMLGLPVAGDRRF
                     SLAAVLGALFEGMAREAALTALRQFRQRLKAAADQAGVQLALEADGQTRAAPENRWCW
                     FEGEDGRVAAAAGFTRAEVEAGARLRMPQSAVEFTERDADGRLVVPYFVSYAHGDDKL
                     VSDLLERLRIRFKSAKEYRFEQWQDKDIVIGSHWRDEIQSAIARCKVGLLLVSPAFLA
                     STFITEQELPQFVAAKADCPEPLKPALPVELKPLDFSGASDLKGLEEIQVFRHNGKSF
                     QARTTDKVKDEFADALYQAILKALKVHLPPSPPPEPSPRRWDEHLRRHLDYKPEAFVP
                     TQGSCIDMEKFQPDASARGPAPERTDALEYLENWLSNPNAQPYCALLGEYGMGKTTTC
                     KALAAHLLDLREQDPSLPLPIYFDLRLVGDLAKEQPVLERIVEITLARSWLGDATRMG
                     SRLNAKEAIRLVCEEGALAIFDGLDEVLVHLPDAAGRRFARELFRILPPAVARKADEK
                     KRPGRLLLSCRSHYFRTLRDEKTFLTAEGRDGIAADDYRALVLLPFTEEQIREYLQHT
                     LPDDDPERVLDLIRSVHNLSEIAERPYTLSLIAQHIPKIERWKLEGRRVSGVMLYREM
                     VLEWLERDAGKHHLTPEHKCELMEHFAAELWRTGEKSWSVGQLEQWLINYLRARLDVA
                     DHYTTKDRELLKEDLRTATFLVREGEDRFRFAHTSLQEYFLACHLQRALLDDQPDAWA
                     LPALNRETLDFLGQLLMEAGLAQAQIGLRNLRDTY"
BASE COUNT          718 a         1255 c         1311 g          661 t
ORIGIN      
        1 cagcagcacc acgtcgcgcc gggtgatctc gtccgccttg cgcggacccc atgcccgcac
       61 gatctccttg agttcgagca gcgcacgctt gcgcgtgctc tcggccagca tcggcaagtg
      121 gcggtcggtg tattccccgg ccagggcgcg caccgtcggg cgccggcgat ccacggcgac
      181 ggcttcggca acggcgttgc gcttacggac ccgttccgca gccgggtcca gcccctcggc
      241 aagctgcgcg cgcaccgctg cgacggcatc gcgcgcggcg gcaagggtcg tttccggata
      301 ggtgccgaag cggatgcgcc ggcgcacgcc ccccagcgtg tagagcagga agaacgacag
      361 cttcccggat ggcagcaccg aaaccccgaa gccctcgcct tcgcgaacct cgcgcggcga
      421 ctccagcccc tcggccgcac gcttggcgag ccgtcgcaac tcaagatcag taagccgttt
      481 catcccgccc ccggaaatgc agcctgccaa gccggacgca agcagaaccg gcttgtggtg
      541 gcgcctgtag tggtacctgt tcagcttagg ctcaggcggg atgcaatgga acaggtcggg
      601 acagcggttg ctgaaaaatc attcagcaca gcaggttatg aagcgtgcgc ggggacaggg
      661 cgggaagcgg tgaaacacca gactggttct catccggaga accgaccggt cggggtctga
      721 tttccactcc gcgggcaggc agccttgccc gtcatctccg ccccggtgca aggccggggc
      781 ggcagaggtt cagcgataca cctcgcgccg atgaccgacg cggatgacct gcacgaccag
      841 ccgggcatcc tcgacggtgt agatcaggcg gtagtcgccg gagcgcaccc gccaggtatg
      901 ccgggcgcca gcgagcttgc gggctccggg cggatacgga tcgcacacaa gcccctcggc
      961 cagcaacacc agtgccgcga tgacctcgcg ggggagcttg cgcagctccc gctcggccga
     1021 gcgcttccag accagctcat aacagtccat cggcccgcaa tccggccttc agctcctcga
     1081 aactgacgca gtcttcgtcc tgccgctcgg cgaccgcggc cagatcggcg atgtcctcca
     1141 gcagcgcctc gtactcggcc agcgacagca cgaccgcgac acgcgcgcca tcggcgtcgg
     1201 tgatgaattg cggatgcagg gactcgaaaa cggtggccat gcagattgcc tccggtggtg
     1261 atgaaggaat gcttcggacc ggcgttcgcg agcgatttaa cacggacgca gcgccgcttg
     1321 ctgcgctgat gcaggaacaa cgcgaaatcg ggcacttcgc acagcgcggc ctcggacact
     1381 ccccctgccg atacggcccg gttcttccag cgtgctcatg tccgccttcc cgctgccatt
     1441 gacgagccag cctgctgatg gcgccgcgcc ccgccaagtc tgcagctttc tgaggtttcc
     1501 gcctgcctga gcacagcggg cggctgaaac ctcgaacttc gtagccattc cgtacttgcc
     1561 tgacgccatc cgtcgtctac gctgatcccg tatcgattcc gtagaccccc ggaggccacc
     1621 atgcccgcga aacccctgca cccggatgtc gtgcgccgca acgccgacaa ggtcgctgag
     1681 cagatggggc gggcggacca ggagcggttc gagcggatgc tgggcttgcc ggtggcgggg
     1741 gatcggcggt tttcgctggc ggcggtgctc ggggcgctgt tcgagggcat ggcgcgcgag
     1801 gcggcgctga cggccttgcg gcagttccgg cagcgcctga aggcggcggc ggatcaggcc
     1861 ggggtgcaac tggcgctcga agccgacggg cagacgcggg cggcgccgga gaatcggtgg
     1921 tgctggttcg agggcgagga cgggcgggtc gcggcggcgg cagggttcac gcgggccgaa
     1981 gtggaagccg gggcgcggct gcggatgccg cagtcggcgg tcgaattcac ggagcgcgac
     2041 gcggacggca ggctggtcgt gccgtacttc gtttcgtatg cgcatggcga tgacaagctg
     2101 gtcagcgatc tgctcgaacg gctgcgcatc cgcttcaaaa gcgcgaagga atatcgcttc
     2161 gagcaatggc aggacaagga catcgtcatc ggcagtcact ggcgcgatga gatccagtcc
     2221 gcgattgcac gctgcaaggt cggcttgttg ctggtcagcc cggcgtttct ggccagcacg
     2281 ttcatcacgg aacaggagct gccgcagttc gtggcggcaa aagcggattg cccggagcca
     2341 ctgaaaccgg cgttgccggt cgaactgaaa ccgctcgatt tcagtggtgc ttccgacctg
     2401 aaagggctgg aggagattca ggtttttcgc cacaacggca agtcgtttca ggcgcgcacg
     2461 accgacaagg tcaaggacga attcgccgat gcgctgtacc aggccatcct gaaagccctg
     2521 aaggtacacc tgccgccttc tccgccgccg gaaccctcgc cacggcgctg ggacgaacat
     2581 ttgcgccggc atctggatta caagcccgag gctttcgtgc ctacgcaggg ctcgtgcatc
     2641 gacatggaga aattccagcc ggatgcgtcc gctcgcggcc ccgcacctga acgcaccgac
     2701 gcactggagt atctggaaaa ctggctgagc aacccgaatg cacagcctta ttgtgcgctg
     2761 ctcggcgaat acggcatggg caagacgacg acctgcaagg cgctggcggc gcatctgctg
     2821 gatttgcgtg agcaagaccc gagcctgccg ctgccgattt attttgatct ccggctggtt
     2881 ggcgatctgg cgaaggaaca gccggtgttg gaaagaatcg tcgaaatcac gctggcgcgc
     2941 agttggctcg gcgatgcgac gcgcatgggt tcacgcctga atgcgaagga agccatccgg
     3001 ctggtatgcg aggaaggcgc gctggcgatc ttcgacgggc tcgacgaggt gctcgtgcat
     3061 ctaccggacg ccgccggccg gcgctttgcg cgcgagctgt ttcgcatcct gccgcctgcg
     3121 gttgcccgca aggccgatga aaaaaaacgt cccggccggc tgctgctgag ttgccgcagc
     3181 cactacttcc gcaccttgcg cgacgagaaa accttcctga ccgccgaagg gcgcgacggc
     3241 atcgcggcgg acgactaccg ggcgctggtg ctgttgccgt tcaccgaaga acagattcgc
     3301 gagtacctgc agcacacgct gccggatgac gaccccgagc gggtgctgga cctgatccgc
     3361 tcggtccaca acctgagcga gattgccgaa aggccgtaca cgctgagtct gatcgcgcag
     3421 cacattccga agatcgaacg ctggaaactc gaaggccggc gcgtttccgg ggtgatgctg
     3481 tatcgggaaa tggtgctgga gtggctggag cgcgacgccg gcaagcacca tctgacgccg
     3541 gagcataaat gcgaactgat ggagcatttc gcggccgaat tatggcgcac cggagaaaag
     3601 tcgtggtcgg tcgggcaact ggagcagtgg ctgatcaatt atctgcgcgc gcgcctcgat
     3661 gtagcggacc attacacgac caaggaccgt gagctgctga aggaagacct gcgcactgcc
     3721 acctttctgg tgcgtgaagg cgaagaccgc ttccgctttg cgcacacgtc cttgcaggaa
     3781 tactttcttg cctgccatct gcaacgcgcg ctgctcgacg accagccgga tgcctgggca
     3841 ttgccggcgc tgaatcggga aaccctggat tttctcgggc agttgctgat ggaagccggt
     3901 ttggcccagg cgcagatcgg cttgcgcaat ctgcgtgaca cctac
//
LOCUS       DAOQJH010000179         3944 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_14070_length_3944_cov_3.921317, whole genome shotgun sequence.
ACCESSION   DAOQJH010000179 DAOQJH010000000
VERSION     DAOQJH010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3944)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3944)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3944
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14070_length_3944_cov_3.921317"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1917)
                     /gene="mrcB"
                     /locus_tag="PLQ18_05325"
     CDS             complement(<1..1917)
                     /gene="mrcB"
                     /locus_tag="PLQ18_05325"
                     /inference="COORDINATES: protein motif:HMM:TIGR02071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1B"
                     /protein_id="HNE01107.1"
                     /translation="MKKPEAPRKRRPSFVRGLFGLIGLCLLAATLVGAVLLYNYASHL
                     DGVIRTQFEGKRWALPARVYARPLEAFVGATLNAEQLQHELDLLRYRRADSIDGPGLY
                     RRNGDTLELSTRGFAFWDGTEASRHVRVGFRDGTLVGLDGLDGQPSPDVLRLEPFEIA
                     GIYPAHHEDRVLVRRQDLPKPLVDALVATEDRTFFEHFGIDLKGVARAAIANLRAGRT
                     VQGASTLTQQLVKNFFLSNERTLTRKLNEMLMAVLIDAHYSKDDILEAYSNEIYLGQD
                     GARAIHGFGRAARFYFDRPLGELTLPQMALMVGLVKGPSYYDPRRHPERAKERRAIVL
                     EAMTELGYIDAVAAAEAKAAPLGVVERQTNGLTLYPNFVDLVQRQLRESYREEDLTSE
                     GLRIFTTLDPRMQDIAEQTLLASLPVLEKSQRMKPDTLQGAVVLASPDNGEVYALVGG
                     RDVRLAGFNRVLDARRPAGSLLKPAVYLAALEDSEHYSLASSLHDEPFTWRIPGGGGD
                     WSPHNYDKRYRGRVMLRDALAKSMNVPTAWIAQQIGMERVVDIMKRLGARQNLRTNPS
                     LVLGAADLTPFEIAQMYQTLASGGFRAPLRAIREVTTKDGKPLSRYPLDIQQVVKPGP
                     AYLIINAMQAVVREG"
     gene            2060..3619
                     /locus_tag="PLQ18_05330"
     CDS             2060..3619
                     /locus_tag="PLQ18_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNE01108.1"
                     /translation="MSPDTTFDTLARSLANPDLYPGADTVRRLDTHISAVFLAGDRAL
                     KIKKPLNLGFLDFGTLQRRAHFCREELRLNRRLAPDIYLDCIPICGSPENPVLGGDPA
                     EAIEWAVSMRRFAQENLLDRCLAEGRLTAAHIDAMARSLAGFHAGIAVAGDDSPFGTP
                     EAVAAPAFDNFRHARELLHAPEDRAALDRIEAWTQATHDRIGPLLAARKAGGRIRECH
                     GDLHLGNMILDGDAVTVFDCIEFNDAFRWIDVAADLAFVVMDLQQRGRDDLAARALNV
                     WLEYSGDHDAAGVLPFYLVYRAMVRAKVAAIRLGQADVPPDEAAAVLAEARRYLALAE
                     RYTQPPQPFLLITHGVSGSGKSTATQTLLEAFGAIRLRSDVIRKQLFGLGPLDDSHAA
                     GSDIYTQKASERTYARLLELAAQLLAQGWPVLVDATFPQAWQRAPFRERAQQAGVPFV
                     LLACSAEADTLRARVAARRQAGTDAAEADVRVLDAQLAGYVAPDADEQPLSAAGLDAA
                     ALTAAVRARLG"
     gene            3625..>3944
                     /locus_tag="PLQ18_05335"
     CDS             3625..>3944
                     /locus_tag="PLQ18_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020680630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HNE01109.1"
                     /translation="MHHTTPGWRRVAIALLLTGSAATAHGQASTPGKTGVPVEVVIEG
                     IEDERLRVNVANFLSIRELASKPEATDSRIAWEHRKAEEEIRTALQPFGYYRPTIQAS
                     LERDG"
BASE COUNT          587 a         1399 c         1357 g          601 t
ORIGIN      
        1 gccctcacgc acgacggcct gcatggcgtt gatgatcaga taggccgggc cgggcttgac
       61 cacctgctgg atgtcgagcg gatagcggct gagcggcttg ccgtccttgg tcgtgacctc
      121 gcggatcgcg cgcagcggcg cacggaagcc gccgctcgcg agcgtctggt acatctgcgc
      181 gatctcgaac ggcgtcaggt cggcggctcc gagcaccagc gacgggttgg tacgcaggtt
      241 ctggcgggcg ccgaggcgct tcatgatgtc gacgacgcgc tccatgccga tctgctgcgc
      301 gatccacgcc gtcggcacgt tcatcgactt ggccagcgcg tcgcgcagca ttacgcggcc
      361 gcggtagcgc ttgtcgtagt tgtgcggcga ccagtcaccg ccaccgccgg gaatgcgcca
      421 ggtgaagggt tcatcgtgca ggctgctcgc gagcgagtag tgctcggaat cctccagggc
      481 cgccagatac acggcgggct tgagcagcga gccggccggg cggcgcgcat cgagcacgcg
      541 gttgaagccg gccagacgca cgtcgcgccc gccgaccagc gcgtagacct cgccgttgtc
      601 ggggctcgcg agcacgaccg cgccctgcag cgtgtcgggc ttcatgcgct gggatttctc
      661 cagcaccggc agcgaggcga gcagcgtctg ttcggcgatg tcctgcatgc gcgggtcgag
      721 cgtcgtgaag atgcgcaggc cctcggaggt caggtcctcc tcgcggtagc tctcgcgcag
      781 ctggcgctgc accagatcga cgaagttcgg atacagcgtc aggccgttgg tctggcgctc
      841 aaccacgccg agcggggccg ccttcgcttc ggccgcagcg acggcgtcga tgtagccgag
      901 ctcggtcatc gcttcgagca cgatcgcgcg gcgttccttg gcgcgctccg ggtgccggcg
      961 cgggtcgtag tacgacgggc ccttgaccag cccgaccatc agcgccatct gcggcagcgt
     1021 cagctcgccg agcggccggt cgaagtagaa gcgcgcggcg cggccgaagc cgtggatggc
     1081 gcgggcgccg tcctgaccga ggtagatctc gttcgaatac gcttcgagga tgtcgtcctt
     1141 cgagtagtgc gcatcgatca gcacggccat cagcatctcg ttgagcttgc gcgtcagcgt
     1201 gcgctcgttc gagaggaaga agttcttgac cagctgctgc gtcagcgtgc tcgcgccctg
     1261 cacggtgcga ccggcgcgca ggttggcgat cgccgcgcgc gccacgccct tgaggtcgat
     1321 gccgaagtgc tcgaagaagg tgcggtcctc ggtcgccacc agcgcatcga cgagcggctt
     1381 cggcaggtcc tggcgacgca ccagcacgcg gtcctcgtga tgtgccgggt agatgccggc
     1441 gatctcgaac ggctccaggc gcagcacgtc gggcgaaggc tggccgtcga ggccgtcgag
     1501 gccgaccagc gtgccatccc ggaaaccgac ccgcacgtgg cgcgaggcct cggtgccgtc
     1561 ccagaacgcg aagccgcgcg tgctcagttc gagcgtatcg ccgttgcgcc ggtacaggcc
     1621 cggaccgtcg atgctgtcgg ccctgcgata gcgcagcagg tcgagttcgt gctgcagctg
     1681 ctcggcgttc agcgtcgcac cgacgaaggc ttcgagcggc cgggcgtaca cgcgcgccgg
     1741 cagcgcccag cgcttgcctt cgaactgcgt gcggatgacg ccgtcgagat ggctggcgta
     1801 gttgtacagc agcaccgcgc cgaccagcgt cgccgcgagc aggcagaggc cgatcaggcc
     1861 gaacaggccg cgcacgaaac tcggacggcg cttgcggggg gcttcgggtt tcttcattcg
     1921 ttcacgttca gggcgcggac gtgccgcgct cggcgcccgc cagcaagcgg cggcgcagcg
     1981 gttgggttgg agccggaggc cggcttattc tgtaggtatc ctgatgcgca tccgatcgcg
     2041 ccgagcccag caagccccca tgagccctga taccacgttt gacacgctgg cccggtccct
     2101 ggcgaacccg gatctctatc cgggcgccga caccgtgcgc cggctggaca cgcatatttc
     2161 cgcggtgttc ctcgccggcg accgggcact gaagatcaag aagccgctga acctcggctt
     2221 cctggacttc ggcacgctgc agcgtcgtgc acacttctgc cgggaggaac tgcggctgaa
     2281 ccgcaggctt gcgccggaca tctacctcga ttgtatcccg atctgcggca gcccggaaaa
     2341 tccggtgctt ggcggtgatc cggccgaagc catcgaatgg gcggtgtcga tgcgccgctt
     2401 cgcgcaggaa aacctgctcg atcgctgcct cgccgaaggc cggctgacgg ccgcgcacat
     2461 cgatgcgatg gcccgcagcc tcgccggctt ccacgccggc atcgccgtcg ccggcgacga
     2521 cagcccgttc ggcacgcccg aagccgtcgc cgccccggcc ttcgacaact tccgccatgc
     2581 gcgcgagctg ctgcacgcgc ccgaagaccg cgccgcgctc gatcgcatcg aggcctggac
     2641 gcaggccacc catgaccgca tcgggccgct gctcgcggcg cgcaaggccg gcggccgcat
     2701 ccgcgagtgc cacggcgacc tgcacctcgg caacatgatc ctcgatggcg atgccgtgac
     2761 ggtgttcgac tgcatcgagt tcaacgatgc cttccgctgg atcgatgtcg ccgccgatct
     2821 cgccttcgtg gtcatggacc tgcagcagcg cggccgcgac gacctcgccg cgcgggcgct
     2881 gaacgtctgg ctcgaataca gcggcgatca cgacgccgcc ggcgtgctgc cgttctatct
     2941 ggtctaccgg gcgatggtgc gcgccaaggt cgcggcgatc cgcctcggtc aggccgacgt
     3001 gccgccggac gaggccgcgg ccgtgctcgc cgaagcgcgc cgctacctcg cgctcgccga
     3061 acgctacacg cagccgccgc agcccttcct gctgatcacg cacggcgtct ccggctccgg
     3121 caagtcgacg gcgacgcaga cgctgctcga agccttcggc gcgatccggc tgcgctcgga
     3181 cgtgatccgc aagcagctgt tcggcctcgg cccgctcgat gacagccacg ccgccggcag
     3241 cgacatctac acgcagaagg ccagcgaacg cacctacgcg cgcctgcttg agctcgccgc
     3301 gcagctgctc gcgcagggct ggccggtgct ggtcgatgcg accttcccgc aggcctggca
     3361 gcgcgcgccg tttcgcgaac gcgcgcagca ggccggtgtg cccttcgtgc tgctcgcctg
     3421 cagcgccgaa gccgacacgc tgcgtgcgcg cgtcgcggcg cgccggcagg ccggcaccga
     3481 cgctgccgag gccgacgtgc gcgtgctcga tgcccagctc gccggctacg tcgccccgga
     3541 tgccgatgaa cagccgcttt ccgctgccgg cctcgatgct gccgcgctga ccgccgccgt
     3601 gcgcgcgcgt ctcggctgac ccggatgcac cacacgacgc cgggatggcg ccgggtcgcg
     3661 atcgcgctgc tgctcaccgg cagcgccgcg actgcccacg gacaggcaag tacgcccggg
     3721 aagaccggcg tgccggtcga ggtcgtcatc gaaggcatcg aggacgagcg cctgcgcgtc
     3781 aacgtcgcga acttcctgag catccgcgaa ctcgccagca agcccgaagc caccgactcg
     3841 cgcatcgcct gggagcatcg caaggccgag gaagaaatcc gcaccgcgct gcagcccttc
     3901 ggctattacc gcccgacgat ccaggcgagc ctggagcgcg acgg
//
LOCUS       DAOQJH010000180         3927 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_14165_length_3927_cov_3.849432, whole genome shotgun sequence.
ACCESSION   DAOQJH010000180 DAOQJH010000000
VERSION     DAOQJH010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3927)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3927)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3927
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14165_length_3927_cov_3.849432"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..543)
                     /locus_tag="PLQ18_05340"
     CDS             complement(<1..543)
                     /locus_tag="PLQ18_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019434564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase N-terminal
                     domain-containing protein"
                     /protein_id="HNE01110.1"
                     /translation="MNPPAHSAPPVSGWQFWIDRGGTFTDLVARSPDGRLLATKLLSE
                     HPERYRDAAVEGIRRLLGLAAGAPLPAGAIEVVKMGTTVATNALLERRGEPVLLVITR
                     GHADALRIAYQNRPQLFARHIVLPAPLYAEVLEADERLAADGSVLKPLDEIALARGFA
                     AARARGLRACAIVFLHGYREP"
     gene            complement(697..2799)
                     /locus_tag="PLQ18_05345"
     CDS             complement(697..2799)
                     /locus_tag="PLQ18_05345"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNE01111.1"
                     /translation="MTMIRRMQDRRLLTLYFAGAFLVLLVAGLLAGVALKTDYQRRMD
                     GAERDSQNLARAFEEHIYRTFRSLDQQSRFLAEYYFRYPDKDELTEALRGLLRIEDAA
                     VQHAIIDEHGILAGSSVGTQSLGINLSDRAHFRHYLQPDATPTYVSKPLVGRASGKAS
                     IQYTRRIEDSDGEFRGVAVVSFDPLYLNSFYQSVDVGERGVISLAGTDGILRARSIDM
                     DQTIGKDISGGELMRSYQSAPAGTYRAPSGVDGIERIVSYRKVRDFPLLVVVGLGLGD
                     VMAGYTGYVASVGGLYGFLLVTLVLLGGVLRNFLRNRTEAERLARETVVERRERQFLS
                     SILQTAGVLVVVAEADGTIKVANPLFARYFGAGSGARAWSRAATGEEFAALVTGLPRS
                     FEAQVSDLGGIRRTVAWTFTAVTDDGGELRNLVGIGLDNTERRAAELMIYQSAKLVTL
                     GEMATGLAHELNQPLNAIKLTVENVLLRSSRGTPEKTYLDDKLDKIRRNVARAASIID
                     HMRIFGRRAERTLGPVDPAQACEGALSITNAPLRLANIACHVICPAGMYWVRAEIGLL
                     EQVLINLILNARDAILSRREQHPETGRADHIELSAQPLADGRMQISVTDTGGGVPEAL
                     MQRIFEPFFTTKPVGQGTGLGLSLSYGIVRDFGGELRVENIEGGACFLVELPAATPAT
                     GPAALPVPAPQAQAQAAQ"
     gene            complement(2796..3167)
                     /locus_tag="PLQ18_05350"
     CDS             complement(2796..3167)
                     /locus_tag="PLQ18_05350"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE01112.1"
                     /translation="MLLLIDDEPDILAEIQEWFEWQGWDCLCAGRLAEAERLLAGRDD
                     IRLVVTDLKLVDGEAFGLIEARLARLPAPDFIIVTGHAGEPEIEHARRIGVQLLHKPL
                     DLAALDAAVAATGHLPRGTPS"
     gene            complement(3201..>3927)
                     /locus_tag="PLQ18_05355"
     CDS             complement(3201..>3927)
                     /locus_tag="PLQ18_05355"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNE01113.1"
                     /translation="IVELENGRLLGFEALLRWQSPRRGLVAPAGFLPALQRSGLAGQV
                     DAWSLVTAATQLARWQRETGRRDLTMSVNLGCAHLAPGEFDRYCAIARQAVRLPAPGT
                     LRIEITEDGPGPQREPYLDGLRALHLAGLRFYLDDYGTGEAALSRLNGLNFEAIKLDR
                     SFVLAPTPENLAICETVIALGRRLGLAVIAEGIEDAAALERLLGFGCRAGQGYWFSPP
                     VPADLAALMLRSQTLTPRVPKRA"
BASE COUNT          558 a         1344 c         1466 g          559 t
ORIGIN      
        1 cggttcgcga tagccgtgca ggaacacgat cgcgcaggcg cgcaggccgc gggcgcgcgc
       61 tgcggcgaag ccgcgcgcga gcgcaatctc gtcgagcggc ttcagcaccg agccgtcggc
      121 ggccagccgc tcgtcggctt cgagcacctc ggcatacagc ggcgcgggca gcacgatgtg
      181 gcgcgcgaac agctgcggac ggttctggta ggcgatgcgc agcgcatcgg cgtggccgcg
      241 cgtgatcacc agcagcaccg gctcgccgcg gcgctccagc agcgcgttgg tcgcgaccgt
      301 cgtgcccatc ttcacgacct cgatcgcccc ggccggcagc ggcgcaccgg cggcgagacc
      361 gagcaggcgg cggataccct cgacggcggc atcgcggtag cgctccgggt gttcggacag
      421 cagcttggtg gcgagcagcc ggccatcggg gctgcgggcg accagatcgg tgaaggtgcc
      481 accgcggtcg atccagaact gccagccgct cacgggcggc gcgctatggg cgggcgggtt
      541 cacgacggcg gcctccggct cggacgggtg caggccgcga ggataccagt cgcaccggcg
      601 acgattgccg ctgtcgccgc tgccggcagt gcttcgcgcg tcgccgccgc agcggcggcg
      661 gacctccgga cggggccgcg cgggcagcca gcccccttac tgcgccgcct gcgcctgtgc
      721 ctgtggtgcc ggcaccggca gcgcggccgg gccggtggcc ggggtcgcgg ccggcagctc
      781 gaccaggaag catgcaccgc cctcgatgtt ctcgacgcgc agttcgccgc cgaaatcacg
      841 gacgatgccg taggacagcg acagcccgag cccggtcccc tgtccgaccg gcttggtcgt
      901 gaagaagggt tcgaagatgc gctgcatcag cgcctcgggc acgccgccgc cggtgtcggt
      961 gacgctgatc tgcatgcggc cgtcggcgag cggctgcgcg ctcagttcga tgtgatcggc
     1021 gcggccggtt tccggatgct gctcgcgccg gctcaggatc gcgtcgcggg cgttcaggat
     1081 caggttgatc agcacctgtt cgagcaggcc gatctcggcg cgcacccagt acatgccggc
     1141 cggacagatc acgtggcagg cgatgttggc gaggcgcagc ggcgcattgg tgatcgacag
     1201 cgccccttcg caggcctggg ccggatcgac cgggccgagc gtgcgttcgg cgcggcggcc
     1261 gaagatgcgc atgtggtcga tgatcgacgc ggcgcgcgcg acgttgcggc ggatcttgtc
     1321 gagcttgtcg tcgagatagg tcttctccgg cgtgccgcgg ctggagcgca gcagcacgtt
     1381 ctcgacggtc agcttgatcg cgttcagcgg ctggttcagc tcgtgcgcga ggccggtggc
     1441 catctcgccg agcgtcacca gcttcgccga ctggtagatc atcagctcgg cggcgcgccg
     1501 ctcggtgttg tcgaggccga tgccgacgag gttgcgcagc tcgccgccgt cgtcggtgac
     1561 cgccgtgaag gtccaggcca ccgtgcgccg gatgccgccg agatcgctga cctgcgcctc
     1621 gaagctgcgc ggcaggccgg tgaccagcgc cgcgaactcc tcgccggtgg cagcacggct
     1681 ccaggcgcgc gcgccgctgc cggcgccgaa gtagcgcgcg aacagcgggt tggcgacctt
     1741 gatcgtgccg tcggcctcgg cgacgacgac cagcacgccg gcggtctgca ggatcgagct
     1801 gaggaactgg cgctcgcggc gctcgaccac ggtctcgcgc gccagacgct cggcctcggt
     1861 gcggttgcgc aggaagttgc gcagcacacc gccgagcagc accagcgtca ccagcaggaa
     1921 gccgtacagg ccgccgaccg aggccacgta gccggtgtag ccggccatca cgtcgccgag
     1981 gccgaggccg acgacgacga gcagcgggaa gtcacggacc ttgcggtagc tgacgatgcg
     2041 ctcgatgccg tcgacgccgc tcggcgcgcg gtaggtgccg gccggtgcgc tctggtagct
     2101 gcgcatcagc tcgccgccgg agatgtcctt gccgatggtc tggtccatgt cgatcgagcg
     2161 cgcgcgcagg atgccgtcgg tgccggccag cgagatgacg ccgcgctcgc cgacgtcgac
     2221 cgactgatag aagctgttca ggtacagcgg atcgaaggac acgaccgcga cgccgcggaa
     2281 ctcgccgtcg ctgtcctcga tgcgccgcgt gtactggatc gaggccttgc cgctggcgcg
     2341 tccgaccagc ggcttgctga cgtaggtcgg tgtcgcgtcg ggctgcaggt aatggcggaa
     2401 atgcgcgcgg tccgaaagat tgatgccgag cgactgcgtg ccgacgctgg aaccggcgag
     2461 gatgccgtgc tcgtcgatga tcgcgtgctg cacggcggca tcctcgatgc gcagcagccc
     2521 gcgcagcgcc tcggtcagct catccttgtc cgggtagcgg aagtagtact cggcgaggaa
     2581 gcggctctgc tggtcgagcg agcggaaggt ccggtagatg tgctcctcga aggcacgcgc
     2641 caggttctgg ctgtcgcgct cggccccgtc catgcgccgc tggtagtcgg tcttcagcgc
     2701 cacgccggcg agcaggccgg cgaccagcag caccaggaac gcgccggcga agtacagcgt
     2761 cagcaggcgg cggtcctgca tgcgacggat catggtcatg acggcgttcc ccgcggcaga
     2821 tggccggtcg cggcgaccgc ggcgtcgagc gcggccagat cgagcggctt gtgcagcaac
     2881 tgcacgccga tgcggcgtgc gtgttcgatt tcgggttcac cggcgtggcc ggtgacgatg
     2941 atgaagtccg gcgccggcag gcgcgcgagc cgcgcctcga tcagcccgaa ggcctcgccg
     3001 tcgaccagct tcaggtcggt gaccaccagc cggatgtcgt cgcgcccggc gagcaggcgc
     3061 tcggcctcgg cgaggcgccc ggcgcacagg cagtcccagc cctgccattc gaaccattcc
     3121 tggatctcgg cgaggatgtc gggttcgtca tcgatcagca gcagcatgtt cggtgctcct
     3181 tgcgggcgtt gcgggcgcgc tcaggcacgc ttcggcacgc gcggggtcag cgtctgcgag
     3241 cgcagcatca gcgccgccag atcggccggc accggcggcg agaaccagta gccctggccg
     3301 gcgcggcagc cgaaaccgag caggcgttcg agcgcagcgg catcctcgat gccctcggcg
     3361 atcaccgcga ggccgagccg gcgcccgagg gcgatgacgg tctcgcagat cgcgaggttc
     3421 tccggcgtcg gcgccagcac gaagctgcga tcgagcttga tcgcttcgaa gttcaggccg
     3481 ttgagccgcg acagcgccgc ctcgccggtg ccgtagtcgt cgaggtagaa gcgcaggccg
     3541 gccaggtgca gcgcgcgcag gccgtcgagg tacggttcgc gctgcgggcc ggggccgtcc
     3601 tcggtgatct cgatgcgcag cgtccccggt gccggcaggc gcacggcctg acgggcgatc
     3661 gcgcagtagc gatcgaactc gccgggcgcg aggtgggcgc agccgaggtt gacgctcatc
     3721 gtcaggtcgc gccggccggt ctcgcgctgc cagcgggcga gctgggtcgc ggccgtgacc
     3781 agcgaccagg catcgacctg tccggcgagg ccgctgcgct gcagcgccgg caggaagccg
     3841 gccggtgcca ccagcccgcg gcgcggactc tgccagcgca gcagcgcctc gaagccgagc
     3901 agccggccgt tctccagctc gacgatc
//
LOCUS       DAOQJH010000181         3921 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_14199_length_3921_cov_3.402225, whole genome shotgun sequence.
ACCESSION   DAOQJH010000181 DAOQJH010000000
VERSION     DAOQJH010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3921)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3921)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3921
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14199_length_3921_cov_3.402225"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..798)
                     /locus_tag="PLQ18_05360"
     CDS             complement(<1..798)
                     /locus_tag="PLQ18_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018315130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HNE01114.1"
                     /translation="MSADDAGPAPIPFTLDGRALDARPGETILSAAARHGIAIPHLCA
                     ADGLAPAGNCRACVVAIDGERTLAPACCRTPAAGMQVQLETERVRKAQRLVLELLAAE
                     TAGAVQKPDSELTHWQQALGVTSTRFAARMQPPADASHPAIRVALDACIQCTRCLRAC
                     RDVQVNDVIGLAARGHDTRIVFDLDDPLAASSCVACGECVQACPTGALLPAREAVSVA
                     ERVVPSVCPYCGVGCQLDYHVAGQRILRVSGRDGPANHGRLCVKGRFG"
     gene            complement(795..2507)
                     /locus_tag="PLQ18_05365"
     CDS             complement(795..2507)
                     /locus_tag="PLQ18_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013033378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HNE01115.1"
                     /translation="MNGRRSGRGGPKGRQVDAQALAEVRETIGELPRERTLLIEHLHA
                     LQDRFRHLAPAHLVALAAELGLAPVEVYEVASFYHHFDVLADGDPPPPGLTVRVCTSL
                     SCELAGAPALAEAIEAAVHAGVRVQRVPCVGRCDVAPLAIVGRNHVPWATVAKVMVRV
                     DDARTEPEHRPVWIDLDGYRAHGGYRLLCDCVEGRRSLDELLATLDAAALRGLGGAGF
                     PAGRKWRTVRSFPGPRCMAVNLDEGEPGTFKDRFWLEQDPHRFFEGLLLAAWAVGADE
                     IYVYTRDEYPVCRRVLAEAHAELLANPPSPCALPVIHFRRGAGAYICGEESAMLESIE
                     GRRGLPRLRPPYVAERGLHGRPTLVQNFETLYWLRDILERGAGWWNGHGRHGRRGLRS
                     FSVSGRVREPGVKLAPAGITLHELIDEYCGGMLPGHALHAYFPGGASGGILPARLAGL
                     PLDFDTLQPYGCFIGSAAVIVLSDQDSVRAAALNAMRFFADESCGQCTPCRVGTAQAA
                     QLMAAPRWDTALLADLAVVLREASICGLGQAAPNTFECALRYFPEEFPELSREARTPG
                     DPAP"
     gene            2695..3297
                     /locus_tag="PLQ18_05370"
     CDS             2695..3297
                     /locus_tag="PLQ18_05370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE01116.1"
                     /translation="MKVDKIRTLKAELKRAFALRLDAVKDARSLNELQRSLADDTTFR
                     DAMTRLMASEDPGMSLTQEPQLLHRLMEKGAARWRELIAAGGGACTVEEVARVTGMSA
                     DAVRKAAQRRQLLAYREGTRLMFPACQFEHGRVLDGLRELAMQLPPGLSDRAVVSFFL
                     SPPGTQESARSPIERLRAGDPAEIEQARQAARLYLEQAAT"
     gene            3294..3905
                     /locus_tag="PLQ18_05375"
     CDS             3294..3905
                     /locus_tag="PLQ18_05375"
                     /inference="COORDINATES: protein motif:HMM:NF020387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HNE01117.1"
                     /translation="MSRLPEVTDDENSLPCRSLPAGSRWHRIARLAHASPLYFDRAAG
                     SRFNSGSATFGTLYLAETLTGALAESLCRNAALRLRCHRHTPLPELAARGDYLVTIGV
                     PMRVLDLTVPNLVRHGLTADLYGEYDARRPVPYRWGPAWADHVHGLGLDGILYPSRHH
                     TACICLAVFERSITDFRHALVAPLDRHPEALRVLDEVFDWAIV"
BASE COUNT          589 a         1347 c         1416 g          569 t
ORIGIN      
        1 gccgaagcgg cccttgacgc acagccggcc gtggttggcc gggccgtcgc ggccgctgac
       61 gcgcaggatg cgctggccgg cgacgtggta atcgagctgg cagccgacgc cgcagtacgg
      121 gcagaccgag ggcacgacgc gctcggcgac gctgacggct tcgcgcgccg gcagcagcgc
      181 gccggtcggg caggcctgca cgcactcgcc gcaggcgacg caggaactcg ccgccagcgg
      241 gtcgtcgagg tcgaagacga tgcgcgtgtc atggccgcgg gcggcgaggc cgatcacgtc
      301 gttgacctgc acgtcgcggc aggcgcgcag gcagcgcgtg cactggatgc aggcatcgag
      361 cgcgacgcgg atcgccggat gcgaggcatc ggccggcggc tgcatgcgcg cggcgaagcg
      421 tgtcgacgtg acgccgagcg cctgctgcca gtgcgtcagt tcggagtcgg gcttctgcac
      481 ggcgccggcg gtttcggcgg cgagcagttc gagcacgagg cgctgggcct tgcgcacgcg
      541 ttcggtttcg agctgcacct gcatgccggc cgccggcgtg cggcagcagg ccggtgccag
      601 cgtgcgctcg ccgtcgatcg cgaccacgca ggcgcggcag ttgccggccg gtgcgaggcc
      661 gtcggcggca cagagatgcg ggatcgcgat gccgtggcgc gccgcggcgc tgaggatcgt
      721 ctcgccgggg cgggcgtcga gcgcacggcc gtcgagcgtg aacgggatcg gggcggggcc
      781 ggcgtcatcg gcgctcacgg ggcggggtct ccgggcgtgc gcgcttcccg ggacagctcg
      841 ggaaactctt ccgggaagta gcgcagcgcg cattcgaagg tgttcggtgc cgcctggccg
      901 agaccgcaga tcgaggcctc gcgcaggacg acggcgagat cggcgagcag tgccgtgtcc
      961 cagcgcggcg cggccatcag ctgcgcggcc tgcgcggtgc cgacgcgaca cggcgtgcac
     1021 tggccgcagg attcgtcggc gaagaagcgc atcgcgttca gcgccgcggc gcgcaccgaa
     1081 tcctgatccg acagcacgat gaccgccgcc gagccgatga agcagccgta gggctgcagc
     1141 gtgtcgaaat cgagcggcag gccggcgagg cgcgccggca ggatgccgcc cgaggcgccg
     1201 cccgggaaat acgcatgcag cgcgtggccg ggcagcatgc cgccgcagta ctcgtcgatc
     1261 agctcgtgca gcgtgatgcc ggccggcgcg agcttgacgc ccggctcgcg cacccggccg
     1321 ctgaccgaga acgaacgcag gccccggcgg ccgtgccggc cgtgaccgtt ccaccacccg
     1381 gcgccgcgtt cgaggatgtc gcgcagccag tacagcgtct cgaagttctg caccagcgtc
     1441 gggcggccgt gcagcccgcg ctcggcgaca tacggcggac gcagccgcgg caggccgcgc
     1501 ctgccctcga tcgattcgag catcgccgat tcctcgccgc agatgtaggc gccggcgccg
     1561 cggcggaagt ggatcaccgg cagcgcgcag ggcgagggtg gatttgcgag cagttcggca
     1621 tgggcctctg cgagcacgcg ccggcacacc ggatactcgt cgcgggtata gacgtagatc
     1681 tcgtccgccc cgaccgccca ggctgcgagc agcaggccct cgaagaagcg gtgcgggtcc
     1741 tgctccagcc agaagcggtc cttgaaggtg ccgggctcgc cttcgtcgag gttgacggcc
     1801 atgcaccgcg gccccggaaa gctgcgcacg gtccgccact tgcgcccggc cgggaagccc
     1861 gccccgccga gcccgcgcag cgccgcggca tcgagcgtcg ccagcagctc atcgaggctg
     1921 cgccgaccct cgacgcagtc gcagagcagc cggtagccgc cgtgggcgcg gtagccgtcg
     1981 aggtcgatcc ataccggccg gtgttcgggc tcggtgcggg catcgtcgac gcgcaccatg
     2041 accttggcca cggtcgccca cggcacgtgg ttgcgcccga cgatcgcaag cggcgccacg
     2101 tcgcagcgcc cgacgcaggg cacgcgctgc acgcgcacgc cggcatgcac ggcggcttcg
     2161 atcgcctcgg ccagcgccgg cgcaccggcc agttcgcagc tcagcgaggt gcacacgcgc
     2221 accgtcagcc ccggcggcgg cgggtcgccg tcggcgagca cgtcgaagtg gtgatagaag
     2281 ctcgcgacct cgtacacctc gaccggcgcc agcccgagtt cggccgcgag tgccacgaga
     2341 tgcgcgggcg ccagatgccg gaagcggtcc tgcagcgcgt gcaggtgctc gatcagcagc
     2401 gtgcgctcgc gcggcagctc cccgatcgtc tcgcgcacct cggccagtgc ctgcgcatcg
     2461 acctgccggc ccttcggccc gccgcgtccg ctccgtcgcc cgttcatgat cgtctggcct
     2521 cgtcgggatg ccggaacaag tgggcaagcg taggtgctcg accggacagt cgcccaggca
     2581 ggcgtgcagg tatccatggc gcccgggctt gtcatgcctg tccatcctgt ccatattgtc
     2641 caggctgtcc cgcaagaatc ggttgaaacc gtggctgact gatcctgagg ctggatgaag
     2701 gtggacaaga ttcgcacgct gaaagccgaa ctgaagcgcg cttttgcgct gcggctcgac
     2761 gctgtcaaag acgcccggtc cctgaacgaa ctgcagcgct cgctggccga tgacacgact
     2821 ttccgcgatg ccatgactcg cctgatggca tccgaagacc ccggaatgtc cctgactcag
     2881 gaaccgcagc tgctgcaccg gttgatggag aaaggtgcag cgcgctggcg tgaactgatt
     2941 gcagcgggcg gcggtgcctg cacggtcgaa gaggttgcgc gtgtgaccgg catgagcgcc
     3001 gatgccgtgc gcaaggcggc ccagcgcagg cagttgctgg cttatcggga gggaacccgg
     3061 ctgatgtttc cggcctgtca gttcgagcat ggccgtgtgc tcgacggcct gcgcgaactg
     3121 gccatgcagt tgccgccggg cctgtcggat cgcgccgtcg tgagcttctt cctgtcgccg
     3181 cccggaacgc aggaaagtgc cagatcgccg atcgaacggc tgcgcgccgg tgatccggcg
     3241 gaaatcgaac aggcccgtca ggccgcccgg ctgtatctgg agcaggccgc gacgtgagcc
     3301 gcttgccgga agtcacggac gatgaaaaca gcttgccctg ccgttccttg ccggccggca
     3361 gccgctggca ccggatcgca aggctggcgc atgcatcacc gctgtatttc gaccgggcag
     3421 cgggcagccg cttcaattca ggcagtgcca ccttcggcac gctgtatctg gcagaaacac
     3481 tgacgggggc gctcgccgaa agcctctgcc gcaatgctgc cctgaggctg cgttgtcatc
     3541 ggcatacgcc gctgccggaa ctcgcggccc ggggcgatta ccttgtcacg atcggtgtac
     3601 cgatgcgggt gctggatctg acggttccga atctggtgcg tcacggcctg acggccgacc
     3661 tctacggcga atacgatgcc cgccggccag taccgtatcg ctgggggccg gcttgggccg
     3721 atcacgttca cggtctcgga ctggacggca tcctgtaccc gtcccggcat cacacggcct
     3781 gcatctgtct ggcggtgttc gagcgaagca tcaccgactt caggcatgcc ctggtcgcac
     3841 cgctcgatcg ccacccggaa gccttgcgcg tgctggacga ggtattcgac tgggccattg
     3901 tctgacgggc ccggctcacc c
//
LOCUS       DAOQJH010000182         3915 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_14226_length_3915_cov_4.334974, whole genome shotgun sequence.
ACCESSION   DAOQJH010000182 DAOQJH010000000
VERSION     DAOQJH010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3915)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3915)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3915
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14226_length_3915_cov_4.334974"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(58..2346)
                     /gene="ptsP"
                     /locus_tag="PLQ18_05380"
     CDS             complement(58..2346)
                     /gene="ptsP"
                     /locus_tag="PLQ18_05380"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006746086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HNE01118.1"
                     /translation="MLDSLQRIVQEVSSAHDLDEALALIVERVKASMNADVCSVYLSD
                     EARREYVLMATDGLRPGAVGRVRIPFGKGVVGLCASRAEPVNLENAPDHPAFIFIAST
                     GEAPFHGFLGVPIVRRRKVMGVLVVQQREQRRYIDEEVAFLVTLAAQLAGSITQAELR
                     QSLDQMDVDDLDTLFVDGVACARGLGIGRALVLFPEARLDSVPDKKLGLDLDLIEAEC
                     SRFRLAVDAELEELKSLAARMGRILSPGDQALFDAYAMLLGSDSLVNGTLERIHAGNW
                     APGALRATVAEYANVFEEMDDAYLRERAADIRDLGGRLLRRLHQNKDAPPVEYPERCV
                     LVGEELNVSQLLDVPPERLVGLVSVRGTGASHVALLARGLGIPAVFGVTGAPLTKLNG
                     RDVVVDGYSARICIQPGTQLREEYERLAREEAELSRGLLELREEPAITPDGQRIHLLA
                     NSGLFADINAARDSGAEGIGLYRSEIHFFLRDRFPGEDEQTATYARVLTSMAPRPVTL
                     RTLDVGGDKPLPYFPIREDNPFLGWRGIRISLDHPDIFKTQLRAMLRAGAATGSTDGL
                     SVLFPMITMVSELDEALELLRQAHAELVEDGVPAHFPKVGVMIEVPAAAYQVELLAKR
                     VDFVSIGSNDLTQYLLAVDRNNDRVAKLYDSLNPAVIGVMQWLVERARRAGKPVSVCG
                     EMAGDPAAALLLIGMGIDSLSMSLGNLLKVKWMIRSVPLSVAQALVADVLGLESAQQI
                     RAAAEEVMIAHGLAGLVRPGQH"
     gene            complement(2413..3039)
                     /locus_tag="PLQ18_05385"
     CDS             complement(2413..3039)
                     /locus_tag="PLQ18_05385"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019624965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HNE01119.1"
                     /translation="MCDPLVNGLQVIDSEGFRANVGIILCNTEGRLFWGRRIGQKSWQ
                     FPQGGIRRHETPEEAMLRELGEETGLRPEHVEILGRTEGWLRYRLPKRLIRRHSKPTC
                     IGQKQVWFLLRLRGCEKDFCLDGSERPEFDHWVWVDWWYPLEAVVAFKRQVYLRALHE
                     LLRFAEQPQLPREPSPAVLARFNLTLQALRRAPAAVLPVRSEPNVRPH"
     gene            3285..>3915
                     /locus_tag="PLQ18_05390"
     CDS             3285..>3915
                     /locus_tag="PLQ18_05390"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015280393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNE01120.1"
                     /translation="MSLALFDLDHTLLAGDSGSLWGRHLCAVGALDAAHFEAENRRFG
                     AEYIAGTLDPLAYFTFAMAPLAAHPIARLLEWREQFIEQHIRPVVLARAVELLDAHRA
                     RGDTLVIVTATDRFVTEPIAALLGVPHLLATEPEQLDGGYTGRVAGEPCFREGKIAAL
                     ERWLGGHGARLDEAWFYSDSQNDLPLLGRVGHPVAVDPDPVLAAEATATG"
BASE COUNT          588 a         1382 c         1363 g          582 t
ORIGIN      
        1 tgaaggcgcg cggcccgcgc gcccggttgg ggcctcccgc cgcagcgctg ccgtgcgtca
       61 gtgctggccc ggacgcacga gtccggccag gccgtgcgcg atcatcacct cctcggcggc
      121 ggcgcggatc tgctgcgcgg attcgagccc gagcacgtcg gcgaccagcg cctgcgcgac
      181 cgacagcggc accgagcgga tcatccactt caccttcagc aggttgccga ggctcatcga
      241 caggctgtcg atgcccatgc cgatcagcag cagcgccgcc gccgggtcgc cggccatctc
      301 gccgcagaca ctgaccggct tgccggcgcg ccgcgcccgc tccaccagcc actgcatcac
      361 gccgatcacc gccgggttca ggctgtcgta gagcttggcg acgcggtcgt tgttgcgatc
      421 gaccgcgagc aggtactgcg tcaggtcgtt cgagccgatc gacacgaaat cgacgcgctt
      481 cgccagcagc tcgacctgat acgccgccgc cggcacctcg atcatcacgc cgaccttcgg
      541 gaagtgcgcc ggcacgccgt cctcgaccag ctcggcgtgc gcctgacgca gcagttcgag
      601 cgcctcgtcg agttccgaca ccatcgtgat catcgggaac agcaccgaca gcccgtcggt
      661 gctgccggtg gcggcgccgg cgcgcagcat cgcgcgcagc tgcgtcttga agatgtccgg
      721 gtgatcgagc gagatgcgga tgccgcgcca gccgaggaag ggattgtcct cgcggatcgg
      781 gaagtacggc agcggcttgt cgccaccgac atcgagcgtg cgcagcgtca ccgggcgcgg
      841 cgccatgctg gtcagcacgc gcgcgtaggt cgcggtctgc tcgtcctcgc ccggaaagcg
      901 gtcgcgcagg aagaagtgga tctccgagcg gtacaggccg atgccctcgg cgccgctgtc
      961 gcgcgcggcg ttgatgtcgg cgaacagccc ggagttggcg agcaggtgga tgcgctggcc
     1021 gtcgggcgtg atcgccggct cctcgcgcag ttcgagcagg ccgcgcgaca gttcggcttc
     1081 ctcgcgcgcc agacgctcgt attcctcgcg cagctgtgtg ccgggctgga tgcagatgcg
     1141 tgcgctgtag ccgtcgacca ccacgtcgcg gccgttgagc ttggtcagcg gcgcgccggt
     1201 gacgccgaac accgccggaa tgccgagccc gcgcgccagc agtgcgacgt gcgaggcgcc
     1261 ggtgccgcgc accgacacca gcccgaccag acgctcgggc ggcacgtcga gcagctgcga
     1321 gacgttgagt tcctcgccga ccagcacgca gcgttccggg tactcgaccg gcggtgcatc
     1381 cttattctgg tgcagccggc gcagcagccg gccgccgaga tcacggatgt cggcggcacg
     1441 ctcgcgcagg taggcgtcgt ccatctcctc gaacacgttc gcgtattcgg cgacggtcgc
     1501 gcgcagcgcc ccgggcgccc agttaccggc gtggatgcgc tccagcgtgc cgttcacgag
     1561 gctgtcggag ccgagcagca tcgcgtaggc atcgaacagc gcctgatcgc cgggcgacag
     1621 gatgcggccc atgcgcgcgg cgaggctctt cagttcctcc agctcggcat cgacggcgag
     1681 gcgaaagcgg ctgcactcgg cctcgatcaa gtcgaggtca aggccgagct tcttgtccgg
     1741 caccgaatcg agccgcgcct ccgggaacag caccagcgcc cggccgatgc cgaggccgcg
     1801 ggcgcaggcc acgccgtcga cgaacagcgt gtcgagatca tcgacatcca tctggtcgag
     1861 cgactggcgc agctcggcct gcgtgatcga gccggcgagc tgcgcggcga gcgtgacgag
     1921 gaaggcgacc tcctcgtcga tgtagcggcg ctgctcgcgc tgctgcacga ccagcacgcc
     1981 catgaccttg cgccggcgca cgatcggcac gccgaggaag ccgtggaacg gcgcctcgcc
     2041 ggtgctggcg atgaagatga aggccgggtg atcgggggcg ttctcgaggt tgaccggctc
     2101 ggcgcggctc gcgcacaggc cgaccacgcc cttgccgaac gggatgcgca cgcgcccgac
     2161 cgcgccgggg cgcaggccgt cggtggccat cagcacgtat tcgcgccgcg cctcgtcgct
     2221 gaggtacacc gagcagacgt cggcgttcat gctggccttg acgcgctcga cgatcagcgc
     2281 cagcgcctcg tccagatcat gcgcggagct gacctcctgc acgatgcgct gcagggaatc
     2341 gagcatgggt accggttcct cggcggaggc agacgaaagg gatgatgagg gtccggtggc
     2401 gaccggaccg cttcagtgcg gccggacgtt gggctcgctg cgcaccggca gcaccgccgc
     2461 cggtgcgcgg cgcagcgcct gcagcgtcag gttgaagcgg gcgagcaccg ccggcgacgg
     2521 ttcgcgcggc agttgcggct gctcggcgaa gcgcagcagt tcgtgcagcg cgcgcagata
     2581 gacctgacgc ttgaacgcga ccacggcttc gagcggatac caccagtcta cccagaccca
     2641 gtggtcgaac tcggggcgtt cggagccgtc gaggcagaaa tccttctcgc agccgcgcag
     2701 gcgcagcagg aaccagacct gcttctggcc gatgcaggtc ggcttcgaat gccggcggat
     2761 cagccgcttc ggcagccggt agcgcagcca gccctcggtg cgaccgagaa tctcgacatg
     2821 ctccgggcgc aggccggttt cctcgccgag ttcgcggagc atggcttctt ccggcgtttc
     2881 gtgacggcgg atgccgccct gcggaaactg ccaggatttc tgcccgatcc ggcgccccca
     2941 gaacaggcgg ccctcggtat tgcagaggat gatgccgacg ttggctctga agccttccga
     3001 gtcaatcact tggagaccgt ttacaagtgg gtcgcagatg tgtgcgttaa cggcaagggt
     3061 aaaccgtggg ttggccggaa acaatggcgt acacagccct gccggcagcg tctgtcaaga
     3121 cggccgcgtg cgccgcccga tggctgccgg atccggcagg aagccgcgaa tttccgggcg
     3181 attcgccgct tcgccgctcc ggagggcgtg attgccgctg cgcgcggcct tccgctacgc
     3241 tcgcgcacct tcatccccgt cacccctgcc ccggaaccgt cgccgtgagc ctcgcgctgt
     3301 tcgacctcga tcacaccctg ctcgccggcg acagcggttc gctgtggggg cggcacctgt
     3361 gcgcggtcgg cgccctcgat gccgcgcact tcgaggccga gaaccgccgc ttcggcgccg
     3421 agtacatcgc cggcacgctc gatccgctgg cctacttcac gttcgcgatg gcgccgctgg
     3481 ccgcgcaccc gatcgcgcgg ctgctcgaat ggcgcgaaca gttcatcgag cagcacatcc
     3541 ggccagtcgt gctcgcgcgc gccgtcgaac tgctcgatgc gcaccgtgcg cgcggcgaca
     3601 cgctggtcat cgtcaccgcc accgaccgct tcgtcaccga gccgatcgcg gcgctgctcg
     3661 gcgtgccgca cctgctcgcg accgaacccg agcagctcga cggcggctac accggccgcg
     3721 ttgccggcga accgtgcttt cgcgaaggca agatcgcggc gctggagcgc tggctcggcg
     3781 ggcacggggc ccggctcgac gaggcctggt tctacagcga ctcgcagaac gacctgccgc
     3841 tgctcgggcg cgtcggccat ccggtcgcgg tcgacccgga cccggtgctg gcggccgagg
     3901 cgaccgcgac cggat
//
LOCUS       DAOQJH010000183         3904 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_14310_length_3904_cov_3.366849, whole genome shotgun sequence.
ACCESSION   DAOQJH010000183 DAOQJH010000000
VERSION     DAOQJH010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3904)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3904)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3904
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14310_length_3904_cov_3.366849"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="PLQ18_05395"
     CDS             <1..196
                     /locus_tag="PLQ18_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011912744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HNE01121.1"
                     /translation="DHNHDNNPPDGSNWELLCLYCHDNEHSRHADLVRGADYSSAAPA
                     PAATFKAFGALADLLKKQDG"
     gene            complement(354..2087)
                     /locus_tag="PLQ18_05400"
     CDS             complement(354..2087)
                     /locus_tag="PLQ18_05400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE01122.1"
                     /translation="MSDHEQDWKRALAAISKDLRRQTAPPPGTSRRIRSSLPAQRTSA
                     RPDATAAAAGPAAAAKSPSPPAPALTPASPARSASSRPAPASPAPARPESPPSGKPPL
                     RGYGRGLSVYATTRKATAPASVPRTAEPITDPLAPSSLAIVPRRLPRQPDTALWRTGG
                     RLLQLDAGPRAARQRMTIGLDFGTAFTKVVVADGANRRYVVNFEAAVRARGAAACFLP
                     GFLAIDSGGLGWLGCDRNNRLLADLKFRLLEETLDAQTRAAIVCYLALVLRYTRHWLL
                     TQHRSVYANRRLEWFVNVGVPAARWRQAGIDDEYRRLVEAAWKLSVAEDEPTLAAAQS
                     VQQERPCLLGERLATVPEFIAQIAMYVNSPQRRPDLHLLVDVGAGTLDVSTFNVHESD
                     GEHVFPIFASRVERLGCHYAIRRALAGTSARVIEDAGFDRQKLAGIKGVTEAELQQRL
                     RTVENDVCRVISAVLTQTKGRRYRNSRAWSDGLPVLLCGGGREVALYRSAIERLIADR
                     YPLRLLHLNRPSNLVAPELPETALQRLSVAYGLSFAFENLGLIRTADTVEDDLPSADA
                     AHTYRDRYIEK"
     gene            complement(2169..>3904)
                     /locus_tag="PLQ18_05405"
     CDS             complement(2169..>3904)
                     /locus_tag="PLQ18_05405"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HNE01123.1"
                     /translation="TSWLGREDALDAESLFECLTLDIDRPQGSPLVRRIAEELAGTVD
                     TAELARHDSKYQRLHEVLIEYLREHPDEKVVVFSYFRPTLRYLARRLEHDGLSACVLM
                     GGMRESKQDVIDRFRDTPQTRVLLSSEVASEGVDLQFCRVLVNYDLPWNPMRIEQRIG
                     RIDRLGQRAESIFVWNLCYAGTIDERIVRRLYERLNLFETALGSLEDVLGDEIARLTQ
                     DLLCQKLTSAEEELRIDQTAQAIEQVRQQQDELERQASTMIAHGGYILEQVNAAHTFR
                     KTITAGDLAAYVRDYLERHAPGHTLYQPGADPFEFDLRLPPAVAVRYANYLQVRHLLG
                     QSRLGTGDEVRCRFENRVGASSHSRIETISQFHPLPRFIGEELERLETSVWPVVALRL
                     DRQRSGLPSLPAPGIYAFALLLTSFSGMRTEEELLALAWPVDADAPLATDLSMDLVNA
                     AKHHGADWPGVAADVDLAGLAERTQDCFERMLQTYAGKRRAMEAANHDRIRFQLDALE
                     NHRRRKLDQIEERLAKLRAAGRTRRVINATEGQRRKLEERIAIQRERLNERNGLKSDR
                     LEVCRGLIRIE"
BASE COUNT          573 a         1283 c         1386 g          662 t
ORIGIN      
        1 cgaccacaac cacgacaaca acccgccgga cggcagcaac tgggagctgc tgtgcctgta
       61 ctgccacgac aacgagcact cgcgccacgc cgatctggtg cgcggcgccg actactccag
      121 cgccgcaccg gcgccggctg cgacattcaa ggctttcggc gcactggccg atctgctgaa
      181 gaagcaggac ggctgaggaa agcgaacggg tccggggttg ccgcgcttgc ccttcacccc
      241 acggctccgc gtccagaaac cctgcgggag ccggtgaaaa tccctcgttc aggtgcgatc
      301 cgtgccagtc ggggtccggc ctgccggtgc cggccgccgg gttcccgcgg ccgtcatttc
      361 tcgatgtacc gatcccggta ggtgtgtgct gcgtcggcgg acggaagatc gtcctccacg
      421 gtatccgcgg tcctgatcag gcccagattc tcgaaggcga acgacagccc gtaggctacc
      481 gacaggcgtt gcagcgcagt ttccggcaac tcgggcgcca ccaggttcga cggacggttc
      541 aggtgcagca gcctgagcgg gtatcgatcc gcgatcagcc gttcgatggc actgcggtac
      601 agggcgacct cgcggccgcc gccgcagagc agcacgggca ggccgtcact ccaggcgcgc
      661 gaattgcggt aacggcggcc tttcgtctgc gtcagcacgg cgctgatcac ccggcagaca
      721 tcgttctcga ccgtgcggag tcgctgctgc agttccgctt ccgtcacgcc cttgattccg
      781 gccagcttct gccggtcaaa gccggcatcc tcgatcacac gtgccgacgt gccggccagt
      841 gcccggcgga tcgcgtaatg acagccgagg cgttcaacgc ggctggcgaa gatcgggaag
      901 acgtgctcgc cgtcgctctc gtggacgttg aaggtggata cgtcgagcgt gccggccccg
      961 acatcgacca gcaggtgcag gtcgggccgg cgctgcggcg agttcacgta catcgcgatc
     1021 tgggcgatga attccggcac ggtggcgagg cgctcaccga gcaggcacgg gcgctcctgc
     1081 tgcacggact gcgcggcggc gagtgtcggt tcgtcctcgg cgacgctgag cttccaggct
     1141 gcctcgacca gtcgccggta ttcgtcgtcg atgccggcct gtcgccagcg cgccgcgggc
     1201 accccgacgt tcacgaacca ctccagcctc cggttcgcgt agacgctgcg atgctgcgtc
     1261 agcagccagt ggcgcgtata gcgcaggacc agggcgagat aacagacgat tgccgcacgg
     1321 gtctgcgcat ccagcgtttc ttcgagcagc cggaacttca ggtcggcgag caggcgattg
     1381 ttccggtcgc aaccgagcca gccgaggccg ccggagtcga tggcgaggaa gccgggcagg
     1441 aagcaggcag ctgcaccgcg cgcccggacc gcggcctcga agttcacgac gtagcggcga
     1501 ttcgcgccgt cggcaacgac gaccttggtg aaggcggtgc cgaaatcgag tccgatggtc
     1561 atgcgctgcc gcgcagcgcg ggggccggca tcgagctgca gcagccggcc gccagtgcgc
     1621 cagagtgccg tgtccggctg ccggggcagg cgccgcggaa cgatggccag gctggagggc
     1681 gcgagcgggt cggtgatggg ctctgctgtc cgggggacgg acgcgggcgc ggtggctttg
     1741 cgggtcgtgg cgtagaccga gaggccacgg ccgtagcccc gcagcggcgg cttgccggag
     1801 ggcggtgact cgggtctggc gggcgccggg gatgctggag ccggcctgga ggatgcggag
     1861 cgggctggcg acgccggagt cagggccggc gcgggcgggg atggtgactt cgcggctgcg
     1921 gccggtcctg ccgcggcagc cgttgcgtct ggccgcgccg acgttcgctg cgccggcagg
     1981 gagctgcgaa tccggcggct cgttcccggg ggcggtgctg tctgccggcg caggtctttg
     2041 ctgatggctg ccagcgcgcg cttccagtcc tgctcgtgat cgctcatgcg tcttctcctg
     2101 actctttctc cagatccctg atgtctgtgt ccatcagtgt tcgtccgtgt ccgtgacgtt
     2161 cgtgcagcct attcgatgcg gatcagtccc cggcacacct ccagccggtc gctcttgagg
     2221 ccgttgcgct cgttcaggcg ctcgcgctgg atcgcaatgc gctcttcgag cttgcgccgc
     2281 tgtccctcgg tggcgttgat gacccggcgc gtgcggccgg cggcccgcag ctttgccaga
     2341 cgttcctcga tctggtcgag cttgcggcgg cggtggtttt ccagcgcatc gagctggaag
     2401 cggatacggt catgattggc cgcttccatg gcgcgacgct tgccggcata ggtctgcagc
     2461 atgcgctcga agcagtcctg cgtacgctcc gccaggcccg ccaggtcgac gtcagcggcc
     2521 acccccggcc agtccgcgcc gtggtgcttg gcggcgttga cgagatccat cgacagatcg
     2581 gttgccagcg gcgcatcggc atcgacgggc caggcgagcg ccagcagttc ctcctcggtg
     2641 cgcatcccgg agaagctggt cagcagcagc gcaaacgcat agatgcccgg tgccggcagc
     2701 gacggcagcc cgctgcgctg ccggtcgagc cgcagtgcca ccaccggcca gacgctggtt
     2761 tcgaggcgct cgagttcctc gccgatgaac cgtggcagcg ggtggaactg gctgatcgtc
     2821 tcgatccggc tgtggctgct ggcgccgacc cggttctcga agcggcagcg gacttcgtcg
     2881 ccggtcccga gccggctttg cccgagcagg tgccggacct gcagatagtt cgcatagcgc
     2941 acggcgaccg ccggcggcag gcgcaggtcg aattcgaacg gatcggcgcc gggctgatac
     3001 agcgtgtggc cgggtgcgtg ccgttcgaga taatcgcgca cataggcggc gaggtccccg
     3061 gcggtgatcg tcttgcggaa ggtgtgggcg gcgttgacct gttcgaggat gtagccgccg
     3121 tgcgcaatca tcgttgaggc ttggcgttcg agttcgtcct gctgctggcg cacctgctcg
     3181 atcgcctgcg cggtctggtc gatgcgcagt tcttcctcag cggacgtcag cttctgacag
     3241 agcagatcct gggtcaggcg ggcgatctcg tcgcccagca cgtcctccag gctgccgagt
     3301 gcggtctcga acaggttcag gcgttcgtac agtcgccgga cgatgcgctc gtcgatggtg
     3361 ccggcataac agaggttcca gacgaagatg ctctcggcgc gctggccgag gcggtcgatg
     3421 cggccgatgc gctgctcgat gcgcatcggg ttccacggca ggtcgtagtt gacgagcacc
     3481 cggcagaact gcaggtccac gccctcgctt gcaacctcgg aggacagcag cacgcgcgtc
     3541 tgcggcgtgt cgcggaagcg gtcgatgaca tcctgcttgc tctcgcgcat gccccccatc
     3601 agcacgcagg cggacaggcc gtcatgctcc agccgccggg caagatagcg cagcgtcgga
     3661 cggaagtatg agaacacgac caccttctcg tccgggtgct cccgcagata ctcgatgagc
     3721 acctcgtgca ggcgctggta cttggagtcg tgccgggcaa gctccgcggt atcgaccgtg
     3781 ccggcgagtt cttcggcgat gcggcgcacg aggggcgagc cttgcggccg gtcgatgtcg
     3841 agggtcaggc actcgaacag gctctcggcg tcgagcgcgt cttcgcgccc cagccagctc
     3901 gtgc
//
LOCUS       DAOQJH010000184         3890 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_14389_length_3890_cov_3.760626, whole genome shotgun sequence.
ACCESSION   DAOQJH010000184 DAOQJH010000000
VERSION     DAOQJH010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3890)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3890)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3890
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14389_length_3890_cov_3.760626"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(326..853)
                     /locus_tag="PLQ18_05410"
     CDS             complement(326..853)
                     /locus_tag="PLQ18_05410"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNE01124.1"
                     /translation="MAVSLRLLETADAPRIQHLVSDPRLASWANLPRPYPADGAERFL
                     QLADCQQRRGTGRHFAIERFGLLVGVISVGCGTDPGLAMLGYWIGVPWQGLGVASRAL
                     DQLLNLDPLAGPGLRTWGSVCLERNLASRRVLEKNGFTAVAARPYEGPYPHRFAGQTC
                     LTYLRMPNPNAVLIA"
     gene            complement(1073..1654)
                     /locus_tag="PLQ18_05415"
     CDS             complement(1073..1654)
                     /locus_tag="PLQ18_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002685087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ANTAR domain-containing protein"
                     /protein_id="HNE01125.1"
                     /translation="MQSLRVILVDPSSTRSAILEQALRDAGLQVIGRFAECDMLVDRI
                     SELQPDIVLVDIESPDRDTLESLQTVGRDNPRPIVVFAEDDSPDAIAAAVRAGVSAYV
                     VDGLQPHRVRPIVEVAIARFREFQALRRELEATRSQLAERKLIDKAKGLLMEHRNQTE
                     AEAYASLRKLAMDRNQRIAEVARNVIDVFELLG"
     gene            1839..2462
                     /locus_tag="PLQ18_05420"
     CDS             1839..2462
                     /locus_tag="PLQ18_05420"
                     /inference="COORDINATES: protein motif:HMM:NF017833.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis protein"
                     /protein_id="HNE01126.1"
                     /translation="MTSFARVFTDRVRTSELLEGYAVHLRGERVALGTLVALLGKRSF
                     GLLLLLFALPNLVPLPPGSSTVFGLPLLLLCAQMMYGRSLPWLPGWLLKKSIPRSSFD
                     SMLARTLPWLRRIERWLQPRLTQCTGRCAERLLGGWCAVQAIVLALPIPLANFPPALC
                     IVLIALGMLERDGVLVIAGGVIGLISLGIALTVGYGLVQGLLLIGQA"
     gene            complement(2523..2771)
                     /locus_tag="PLQ18_05425"
     CDS             complement(2523..2771)
                     /locus_tag="PLQ18_05425"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HNE01127.1"
                     /translation="MTVEQIRATILRVLSEVAPNADVSDVNPNVSFHDQFEIDSIDFL
                     RLMTSLEKELQVTILDFDYPKLSTLQGCEDYLRPQLAA"
     gene            complement(2985..>3890)
                     /locus_tag="PLQ18_05430"
     CDS             complement(2985..>3890)
                     /locus_tag="PLQ18_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013651837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNE01128.1"
                     /translation="IDEAEFDAQRWYRILDEQQVNIWYTAPTAIRMLMKLGDAPMAGR
                     TLPALRLMASVGEPLNPEAVLWGQRVFGQPFHDTWWQTETGAIMVANYGSEPVAPGSM
                     GRPLPGIDAAIVHRRRDGGLDFLPDGEAGELAIRAGWPSMFRAYLDEPERYASCFADG
                     WYLSGDRAQRDADGRYWFVGRADDVIKTSGHLIGPFEVESVLLEHPAVAEAGVIGKPD
                     PWAGELIKAFVALKPGHTGDDALRKSLIAHARTRLGPAVAPRELGFVDSLPKTRSGKI
                     MRRLLKARELGLPEGDLSTLEGHES"
BASE COUNT          615 a         1302 c         1317 g          656 t
ORIGIN      
        1 ccagcgcctc gctgccatcc gcggcggttt ccagccggta gccgtccccg gcgagcatgg
       61 cttcgagcgc caggcgggca agggcgtcgt catcgacgag cagcagcagc ggcggcagat
      121 catcgtcggc cggcacggtg gcggactgtg gcggcagcgg cggttccatc gagaatccgg
      181 cagtaggcag cgcaagggca gcaccggcct gaccagccgc tgcatgcctg ccgattccgg
      241 acaatccgga cctgcctgtc catgatccgg gtcagttccg gcccgggtgg gatctgccat
      301 ggctgccggc atggcggctg ccggttcagg cgatcagcac cgcgttcggg ttcggcatgc
      361 gcaggtaggt caggcaggtc tggccggcga agcggtgcgg gtacgggcct tcgtagggac
      421 gggcggcgac ggcggtgaag ccgttcttct cgagcacgcg gcgactggcg aggttgcgct
      481 ccagacagac cgatccccag gtgcgcagcc ccggtccggc cagcggatcg agattcagca
      541 gttgatcgag cgcacggctg gcaacgccga gtccctgcca cggcaccccg atccagtagc
      601 cgagcatcgc cagccccgga tcggtaccgc agccgaccga gatcacgccg accagcagcc
      661 cgaagcgctc gatcgcgaag tgccgaccgg tgccacggcg ctgctggcag tcggcgagct
      721 gcaggaagcg ctcggcgcca tcggccggat acggccgcgg caggttggcc caggaggcca
      781 ggcgcggatc ggagacgaga tgctggatgc gcggcgcatc ggcggtttca agcagtcgca
      841 gactgacggc catggcgaag gtctcccaca ggcagaatgt ggcattcccc cttcttcccc
      901 caccccgcca acatagccag ccgacgaccg gccaaatgca gaagtttccg gaaatcagtc
      961 cggtaaacga tttttcatga tcgatccctg aagcttcctg cctgtttccg tcatccgtgg
     1021 agattccgtg gagacaccgc gccgccatgc gcgcagtgcc gggcgcagcc gctcagccca
     1081 gcagttcgaa cacatcgatg acgttgcgtg cgacctcggc gatgcgctgg ttgcggtcca
     1141 tcgcgagctt gcgcagcgac gcataggctt cggcctcggt ctggttgcgg tgttccatca
     1201 gcaggccctt ggccttgtcg atcagcttgc gctcggcgag ctggctgcgc gtggcttcga
     1261 gttcgcggcg cagcgcctgg aactcccgga agcgcgcgat cgcgacctcg acgatcggcc
     1321 gcacgcggtg cggctgcaga ccgtcgacga cgtaggcact gaccccggcg cgcacggcgg
     1381 ccgcgatggc atccggggag tcatcctcgg cgaacacgac gatcggccgc gggttgtcac
     1441 gcccgaccgt ctgcaggctc tccagcgtgt cgcggtccgg cgattcgatg tcgaccagca
     1501 cgatgtcggg ctgcagttcg gagatccggt cgaccagcat gtcgcactcg gcgaagcggc
     1561 cgatcacctg caggccggca tcgcgcaggg cctgctccag aatggcggaa cgggtggaac
     1621 tgggatcgac gaggatgacg cgcagcgact gcatggcaga actccagggc tgtcccgaaa
     1681 aattaatgca ttagtcatgc cattacggta caagatttcg gatcgaatgc cgtgccgatg
     1741 cacgcagcca cgctccgatc gccctgcttc cgcacgccgg cctccggctg cggtcagtcc
     1801 tgaatcgcgc attcctccgg ccaccgggaa ccagacgcat gacatcgttc gccagggtct
     1861 tcaccgaccg ggtccgcacc tccgaactgc tcgaaggcta tgccgtacac ctgcgcggcg
     1921 agcgcgtcgc gctcggcacg ctggtcgcgc tgctcggcaa gcgctcgttc gggctgctgc
     1981 tgctgctgtt tgccctgccg aacctggtgc cgctgccgcc gggctcgtcg acggtgttcg
     2041 gcctgccgct gctgctgctc tgcgcgcaga tgatgtacgg ccgctcgctg ccgtggctgc
     2101 cgggctggct gctgaagaag tcgatcccgc gcagcagctt cgattcgatg ctggcacgca
     2161 cgctgccgtg gctgcgccgc atcgaacgct ggctgcagcc gcgactgacg caatgcaccg
     2221 ggcgctgcgc cgaacggctg ctcggcggct ggtgtgcggt ccaggccatc gtgctggcgc
     2281 tgccgatccc gctcgccaac tttccgccgg cgctctgcat cgtgctgatc gcgctcggca
     2341 tgctggaacg cgacggcgtg ctggtgatcg ccggcggcgt gatcgggctg atcagcctcg
     2401 gcatcgcact gacggtcggc tacggcctcg tgcagggtct gctgctgatc ggccaggcct
     2461 gaggccgcag cggcaccggc ggctcgcgga ccgccggtgc cggaaggcat cgccggcccg
     2521 actcaggccg cgagctgcgg ccgcaggtag tcctcgcagc cctgcagcgt cgacagcttc
     2581 gggtagtcga agtcgaggat cgtgacctgc agttcctttt cgaggctggt catcagccgc
     2641 aggaagtcga tcgagtcgat ctcgaactgg tcatggaagc tgacgttcgg attgacgtcg
     2701 ctgacgtcgg cgttcggggc gacctcgctg aggacgcgca ggatggtggc tcggatctgt
     2761 tcgacggtca tggcggtata cctcgggggg aagcgcaaga agcaggtttg attaattctg
     2821 cttcacatta taaccccgga acccggttat gctgcaacgc aatatctgcc gcgcctgctt
     2881 gcaccatatc gcgcaagcca ttgtttataa aacaaatcat tcgcacgaat ctggtgcaaa
     2941 cattggtcat accagttccg gccgcccgga agtgcgattt tttgtcaaga ctcgtgacct
     3001 tcgagcgtcg acaggtcgcc ttcgggcagg ccgagttcgc gcgccttcag cagccggcgc
     3061 atgatcttgc cgctgcgcgt cttcggcagg ctgtcgacga agccgagctc gcgcggcgcc
     3121 accgccggcc cgagccgggt gcgggcatgt gcgatcagcg acttgcgcag cgcatcgtca
     3181 ccggtatggc cgggcttcaa tgccacgaag gccttgatca gctcgccggc ccacggatcg
     3241 ggcttgccga tgacgccggc ctcggcgacg gccggatgtt cgagcagcac gctttcgacc
     3301 tcgaacggcc cgatcaggtg gcctgaagtc ttgatcacgt catcggcgcg gccgacgaac
     3361 cagtagcggc cgtcggcatc gcgctgcgcg cggtccccgg acagatacca gccgtctgcg
     3421 aagcagctgg cataacgctc gggctcgtcg agataggcgc ggaacatcga cggccagccg
     3481 gcgcggatcg ccagttcgcc ggcctcgcca tcgggcagga aatcgaggcc gccgtcgcgc
     3541 cggcggtgca cgatcgcggc gtcgatgccc ggcagcggcc ggcccatcga gcccggagcc
     3601 accggctcgc tgccgtaatt ggccaccatg atcgcgccgg tctcggtctg ccaccaggtg
     3661 tcgtggaagg gctggccgaa cacgcgctgg ccccacagca cggcctcggg gttcagcggt
     3721 tcgccgacgc tcgccatcag ccgcagcgcc ggcagcgtgc gcccggccat cggcgcgtcg
     3781 ccgagcttca tcagcatgcg gatcgcggtc ggcgcggtgt accagatgtt gacctgctgc
     3841 tcgtcgagga tgcgatacca gcgctgcgcg tcgaattcgg cctcgtcgat
//
LOCUS       DAOQJH010000185         3887 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_14415_length_3887_cov_3.302714, whole genome shotgun sequence.
ACCESSION   DAOQJH010000185 DAOQJH010000000
VERSION     DAOQJH010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3887)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3887)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3887
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14415_length_3887_cov_3.302714"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..828
                     /locus_tag="PLQ18_05435"
     CDS             <1..828
                     /locus_tag="PLQ18_05435"
                     /inference="COORDINATES: protein motif:HMM:NF013322.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HNE01129.1"
                     /translation="LKDRHAVTEQWFSVQMPGREAPMPCGFTGSRGIDGVEVLAVTRN
                     SRKLRRGALAGNRFRIVVRELDGPPAAVLDRLPAITARGVPNWFGEQRFGRDGGNLER
                     ARRLLAGTLRERDRHKRGLYLSAARSHLFNRYLAARIIDGSWDQALPGEVLMLDGSHS
                     VFACPQPDATILERLARHDLHPSGPLWGAGESLATAAALAGETALAAAEAQLCAGLAA
                     EGLKQERRALRLVPAQLHGEAIGEAAVQFEFTLPAGAYATTVLHELVGNPEAQDDML"
     gene            880..1605
                     /locus_tag="PLQ18_05440"
     CDS             880..1605
                     /locus_tag="PLQ18_05440"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNE01130.1"
                     /translation="MMTAAAGTLLNRESAYTTPRADVSALVPREAMRILDVGCSNGAL
                     GASLRAEVAGRRVVGIELDAGFARQAALLLDRVEQADLDAFDWSDGLGDERFDCIVFA
                     DVLEHLVDPQRHLRGALERLDAGGCIVLSLPNIRHVSALASIYGHGRFPRRDRGIFDA
                     THLRWFTIADGRSLLADTGLQIDAEGFVLRAGDRGGGFLNRMLNRLPDALQRWAPVRE
                     FLTYQFSLRARRPAALPETVPMS"
     gene            1610..2713
                     /locus_tag="PLQ18_05445"
     CDS             1610..2713
                     /locus_tag="PLQ18_05445"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNE01131.1"
                     /translation="MSASGRRIGFVLCSDAAAPLPSTRISVFNVLPALRAAGYDPRIA
                     FAPPQPTERPDVSGLAARLIADGIDIAYFQKVHGPGVRAEIETLRQAGVRTVYGVCDR
                     IDDDMVRLTDATVIVTDYLKQQHAPELQARIHVVHDGIEHPEVRCREDIAAGDGRLRA
                     ILVTSGSPETVPVLGRPPRWLALTVVGQYPERETPLQALRARLRRVRHAPPGERLERL
                     RGYAFTARAWQPQRVYEDLAGAEVGIIPVDMRPDPLPGRQVSYWQVKSENRLTLKMAA
                     GLAVVASPVPSYLDIIEQGVNGYIAHSRADWLAALDALRDPQHRHSVGAAARASVLQR
                     YSIDEQARRLIAVFDGLRGTSQPAGDAALLRMP"
     gene            complement(2723..3268)
                     /locus_tag="PLQ18_05450"
     CDS             complement(2723..3268)
                     /locus_tag="PLQ18_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018231331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HNE01132.1"
                     /translation="MPADRPEPPADEVEAFRQAIGPIEPHRCDLVEPWRAPRPPLPLQ
                     KLADEKQVLRDMVSDHFEPADLETGEHLSYSREGVQDGVLRKLKRGQYSLGAQLDLHG
                     LTWAEARVTVHAFLSLVQRDRVHCVRIVHGKGNRSRHLGPVLKQKLNVYLRLRDDVLA
                     FCSARPDDGGTGALYVLLRSR"
     gene            3388..>3887
                     /gene="surE"
                     /locus_tag="PLQ18_05455"
     CDS             3388..>3887
                     /gene="surE"
                     /locus_tag="PLQ18_05455"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017366107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0008254 - 3'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HNE01133.1"
                     /translation="MRILVSNDDGYLAPGIRALAQALAGIAEITVAAPDRNRSGASNS
                     LTLDRPLSVVRHPDGVWSLDGTPTDCVHVALTGLMEHEPDMVVSGINAGANLGDDVLY
                     SGTVAAAMEGRFLGRPAMAVSLCAYEPDHLDTAARVALHLVGRLCRQPLPADTMFSPP
                     VLARGD"
BASE COUNT          527 a         1377 c         1393 g          590 t
ORIGIN      
        1 ctcaaggacc gccacgccgt caccgagcag tggttctcgg tgcagatgcc ggggcgcgag
       61 gcgccgatgc cgtgcggctt caccggcagc cgcggcatcg acggcgtcga ggtgctggcc
      121 gtcacccgca acagccgcaa gctgcgtcgc ggtgcgctgg ccggcaaccg cttccgcatc
      181 gtcgtgcgcg agctcgacgg cccccctgcc gccgtgctcg atcgcctgcc ggccatcacc
      241 gcccgcggcg tgccgaactg gttcggcgaa cagcgcttcg gccgcgacgg cggcaatctc
      301 gaacgcgcgc ggcggctgct cgccggcacg ctgcgcgagc gcgaccgtca caagcgcggc
      361 ctgtacctgt cggcggcccg ctcgcacctg ttcaaccgct atctcgccgc acggatcatc
      421 gatggcagct gggatcaggc gctgccgggc gaggtgctga tgctcgacgg cagccattcg
      481 gtgttcgcgt gcccgcagcc ggacgcaacc atcctggagc gcctggcccg gcacgacctg
      541 catccgagcg gcccgctgtg gggcgccggc gaatcgctgg cgacggcggc ggcgctggcc
      601 ggcgagacgg cgctggccgc ggccgaggca cagctctgcg cgggactcgc ggccgaaggg
      661 ctgaagcagg agcggcgtgc gctgcggctg gtgccggcgc aactgcacgg cgaggcgatc
      721 ggcgaggcgg ccgtgcagtt cgaattcacg ctgcctgccg gcgcctacgc gacgacggtg
      781 ctgcatgaac tggtcggtaa tccggaggcg caggacgaca tgctataaac ccggcgtacc
      841 ggtgcgaccg gtcctgtcag ccccggagaa tccacgcaga tgatgaccgc cgccgcggga
      901 acccttctga atcgcgagtc ggcctatacg acgccgcgcg ctgacgtttc cgccctggtg
      961 ccgcgcgagg ccatgcgcat cctcgatgtc ggctgcagca acggtgcgct cggtgccagc
     1021 ctgcgcgccg aggtggcggg ccggcgggtc gtcggcatcg aactcgatgc cggcttcgcc
     1081 cgtcaggccg cgctgctgct cgatcgggtc gaacaggccg atctcgatgc cttcgactgg
     1141 tcggacggtc tcggcgacga gcgcttcgac tgcatcgtgt tcgccgacgt gctcgaacac
     1201 ctcgtcgatc cgcagcgcca cctgcgcggc gcgctggagc gtctcgatgc cggcggctgc
     1261 atcgtgctca gcctgccgaa catccggcac gtctcggcgc tcgcgagcat ctacggtcat
     1321 ggccggtttc cgcgccgtga ccggggcatc ttcgatgcga cccacctgcg ctggttcacg
     1381 atcgccgacg gccgcagcct gctcgccgat accggtctgc agatcgacgc cgagggcttc
     1441 gtgctgcgcg ccggggatcg cgggggcggt ttcctgaatc gcatgctgaa ccgcctgccc
     1501 gacgcgctgc agcgctgggc gccggtgcgc gagttcctga cctaccagtt cagcctgcgt
     1561 gcgcgccgtc cggcggcgct gcccgaaacg gtcccgatgt cctgacgcga tgagtgcttc
     1621 gggcaggcgg atcggtttcg tgctgtgctc ggatgcggcg gcaccgctgc cgagcacgcg
     1681 catctcggtg ttcaacgtgc tgccggcgct gcgtgcggcc ggctacgacc cccggatcgc
     1741 cttcgcaccg ccgcagccga ccgagcgtcc ggacgtgagc gggctggcgg cgcgcctgat
     1801 cgccgacggc atcgacatcg cctacttcca gaaggtgcac ggccccggcg tgcgcgccga
     1861 gatcgaaacc ctgcgccagg ccggggtgcg caccgtctac ggtgtctgtg accgcatcga
     1921 cgatgacatg gtgcgtctga ccgacgcgac ggtcatcgtc accgactacc tgaagcagca
     1981 gcacgccccg gaactgcagg cgcgcatcca cgtcgttcac gacggcatcg agcacccgga
     2041 ggtccggtgc cgggaagaca tcgccgccgg cgacggccgg ctgcgcgcga tcctcgtgac
     2101 ctcgggctcg cccgaaaccg tgccggtgct cggacggccg ccgcgctggc tggcgctgac
     2161 cgtcgtcggc cagtatccgg agcgcgagac cccgctgcag gcgctgcgcg cgcgcctgcg
     2221 ccgcgtccgc cacgcgccgc ccggcgaacg cctggagcgg ctgcgcggct acgccttcac
     2281 ggcgcgggcc tggcagccgc agcgggtcta cgaggatctc gcaggcgccg aggttggcat
     2341 cattccggtc gacatgcgtc cggacccgct gccgggccgg caggtctcgt actggcaggt
     2401 caagtccgag aaccggctga cgctgaagat ggcggccgga ctggccgtgg tcgcgagccc
     2461 ggtgccgagc tatctcgaca tcatcgagca gggtgtcaac ggctacatcg cccactcgcg
     2521 cgccgactgg ctggcagccc tcgacgcgct gcgcgatccg cagcaccggc acagcgtcgg
     2581 cgctgcggcc cgggcgagcg tgctgcagcg ctattcgatc gacgaacagg cgcgccggct
     2641 catcgccgtg ttcgacggtc tgcgcggcac ctcgcagccg gccggcgacg cagcgctgtt
     2701 acgaatgccg tagccccggg tctcagcggc tgcgcagcag cacgtacagc gcgccggtgc
     2761 cgccgtcgtc gggccgggcc gagcagaagg ccagcacgtc atcgcgcagc cgcaggtaga
     2821 cgttcagctt ctgtttcagc accggcccga ggtggcgcga gcggttgccc ttgccatgca
     2881 cgatgcgcac gcaatgcacg cggtcgcgct gcacgaggct caggaacgca tgcacggtca
     2941 cgcgcgcctc ggcccaggtc aggccgtgca ggtcgagctg ggcgccgagg ctgtactgac
     3001 cgcgcttgag cttgcgcagc acgccatcct gcacgccttc gcggctgtag ctcaggtgct
     3061 cgccggtttc gagatcggcc ggctcgaagt ggtccgagac catgtcgcgc agcacctgct
     3121 tctcgtcggc gagcttctgc agcggcagcg gcgggcgtgg cgcgcgccag ggctcgacga
     3181 gatcgcagcg gtgcggctcg atcgggccga tggcctgacg gaaggcttcg acctcgtcgg
     3241 cgggtggttc agggcggtcg gcaggcatcg ggcgggcgtt tttgactttg ccggggcgat
     3301 gtgtctttga taccggccgg tttgcgccgc cggcaaggct gcgtgacatg gggcagggct
     3361 gcggcgcgcg gacggggtga gcggtcgatg aggatactcg tcagcaacga cgacggatat
     3421 ctggcacccg gcatccgggc gctggcgcag gcgctcgccg gaatcgccga gatcaccgtg
     3481 gccgcgccgg accgcaaccg ctccggggcc agcaattccc tgacgctcga ccggccgctc
     3541 agcgtcgttc gtcatcccga tggcgtctgg agcctcgacg gcacgccgac cgactgcgtg
     3601 cacgtggcgc tgaccggcct gatggagcac gagccggaca tggtcgtctc cggcatcaac
     3661 gccggggcca atctcggcga tgacgtgctg tattcgggta cggtcgctgc ggcgatggag
     3721 gggcgcttcc tcggccggcc ggcgatggcc gtgtcgctgt gcgcctacga acccgatcat
     3781 ctcgacacgg ccgcgcgcgt agcgctgcat ctggtcgggc ggctgtgccg ccagccgctg
     3841 ccggccgaca ccatgttctc gccgccagtt ctcgcacgcg gcgattg
//
LOCUS       DAOQJH010000186         3885 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_14427_length_3885_cov_4.134726, whole genome shotgun sequence.
ACCESSION   DAOQJH010000186 DAOQJH010000000
VERSION     DAOQJH010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3885)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3885)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3885
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14427_length_3885_cov_4.134726"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(156..1151)
                     /locus_tag="PLQ18_05460"
     CDS             complement(156..1151)
                     /locus_tag="PLQ18_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GcvA"
                     /protein_id="HNE01134.1"
                     /translation="MAYRLPPLNALRAFEAAARHLSFSKAAEELFVTPAAISHQIKSL
                     EEFLGVQLFRRMNRALALTDAAQACLPRLRQGFDALSEAVDLVRAQEDSGLLTVSLTP
                     SFAVKWLVPRLDRFSALHPNIEMRIAASSRQLDLRGGGQRSSTDLVDFRREDVDLAIR
                     FGSGDYPDFHVTKLFSVHATPLCSPALLKRNPLNEPADLAQHVLLHDETVYGPEYTPE
                     REPMWRVWLRAAGVADRVDARRGPHFSSASLTIEAALDGQGVALGLPVLALADIQTGR
                     LVAPFGRQVPIEFAYWIVCPTITAQRPKIVAFRKWLLDEARRDEALLAQISAGGS"
     gene            1365..1625
                     /locus_tag="PLQ18_05465"
     CDS             1365..1625
                     /locus_tag="PLQ18_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016490328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HNE01135.1"
                     /translation="MFSRFVVHPGAGHLVNAISGSGLMVPVRVDIESPSRFVGALKLL
                     RLWVMRARERRALVRLDEHALTDIGMSRADALSEAAKPFWRR"
     gene            complement(1995..3329)
                     /locus_tag="PLQ18_05470"
     CDS             complement(1995..3329)
                     /locus_tag="PLQ18_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005220922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNE01136.1"
                     /translation="MNPTVLVVDDEPHVRLAAVQTLELEGITAEPLASAAAALARLSP
                     EFDGIVVSDVRMPGMDGLALQRAVAALDPELPVVLVTGHGDIAMAVQAIRDGAYDFLE
                     KPFRTEALLDIVRRGLDKRALTLENRRLRRELAAHREGDALLLGRSPGIARLRAAIDN
                     LADTGADVLIEGETGAGKEVVARLLHQRSHRRGQAFVALNCGAIPETIFESELFGHEA
                     GAFTGASGRRVGKLEHAHGGTVFLDEIESMPLALQVKVLRALQDRMIERLGSNRGVAL
                     DIRVVAATKVDLRVAAREGRFREDLYYRLNVVKLAIPPLRERREDIPLLFRHFVLKAA
                     ERYRREPPEVAGDTLAALMSEPWPGNVRELANAADRFVLGHLLGPAALAAGAPAQSLP
                     DQVAAFERVVIVQELERQDGNVVATADALGVPRKTLYDKLKKYGLRREDGFR"
     gene            complement(3326..>3885)
                     /locus_tag="PLQ18_05475"
     CDS             complement(3326..>3885)
                     /locus_tag="PLQ18_05475"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNE01137.1"
                     /translation="AARLTQQLLAFGRKQVLQPRRVSLNQLVREAMSGLAPMLKPRIT
                     VVEQLAPDDPHVLVDRQQFQHVFLNVLLNALEAMPDGGTLTLNTSQEPFAPAEGDSGA
                     PGRYGVLRIRDTGAGMDAGTLERAFEPFFTTKPVGEGLGLGLSISYGIVQNLGGTIRA
                     ENLAGGGACFTVELPLPAPQPLESA"
BASE COUNT          535 a         1325 c         1430 g          595 t
ORIGIN      
        1 ttgtcccgcg ctgcccggcc gttctcccgc caggggaggg gctggccggg cggtgtgcgg
       61 gaaaccggtg gccgggtgtc cgcgagcggc atggggtgtt cccggcgtcc gccgccgctc
      121 cgtatccggc gcgctccggc gcgctccgtc atgcgctagc tgccgcccgc actgatctgc
      181 gcgagcagcg cctcgtcgcg tcgcgcctca tcgagcagcc acttgcggaa cgcgacgatc
      241 ttcgggcgct gcgcggtgat cgtcgggcag acgatccagt acgcgaactc gatcggcacc
      301 tgccggccga acggggcgac cagccgcccg gtctggatgt cggccagcgc cagcaccggc
      361 aggccgagcg cgacgccctg accgtcgagt gcggcctcga tggtcagcga ggcgctgctg
      421 aagtgcgggc cgcgccgggc atcgacccgg tcggcgacgc cggccgcacg cagccacacg
      481 cgccacatcg gttcgcgctc cggcgtgtat tcggggccgt agacggtctc gtcgtgcagc
      541 agcacgtgct gtgcgagatc ggccggctcg ttcagcgggt tgcgcttcag cagcgccggg
      601 ctgcagagcg gcgtcgcgtg caccgagaac agcttggtga cgtggaaatc cgggtagtcg
      661 ccgctgccga agcggatcgc gaggtccacg tcctcgcgcc ggaaatccac cagatcggtg
      721 ctgctgcgct gaccgccgcc gcgcaggtcg agctggcggc tgctggcggc gatgcgcatc
      781 tcgatgttcg ggtgcagcgc cgagaagcgg tcgagccgcg gcaccagcca cttgaccgcg
      841 aacgatggcg tcaggctgac ggtcagcagg ccgctgtctt cctgcgcgcg cacgagatcg
      901 acggcctcgg acagcgcatc gaagccctgg cgcagccgcg gcaggcaggc ctgcgcggcg
      961 tcggtcagcg ccagcgcgcg gttcatgcgc cggaacagct gcacgccgag gaactcctcc
     1021 agcgacttga tctggtggct gatcgccgcc ggcgtcacga acagttcctc ggcggccttg
     1081 ctgaaactca gatggcgggc ggcggcttcg aaggcgcgca gggcgttcag gggcggcagg
     1141 cgataggcca tcttcgttaa acccggattt gtgcgagggt gagattttgt cgtttgcagg
     1201 cgctgcgggc gcggaccagc attgcaccaa caaagagcgg tactgtttca aattggtgca
     1261 aatcgttaca agtcattggt tggtcgtcgc gtcacaggga tggtgtgaac cgaaacagtg
     1321 cattgacgcg aggcgaccga tcggggctgt tgcaggtggt catcatgttc agtcgtttcg
     1381 tcgttcatcc gggtgccggt catctcgtca acgccatcag cggcagcggc ctgatggtac
     1441 cggtgcgcgt cgacatcgaa tccccgtcgc ggttcgtcgg cgcgctgaaa ctgctgcggc
     1501 tgtgggtgat gcgcgccagg gagcgccgcg ccctggtccg gctcgacgag cacgcactca
     1561 ccgacatcgg catgagccgc gccgatgcgc tgagcgaggc cgccaagccg ttctggcgtc
     1621 gctgaggcgc gtccgggcga gcggcagcgg gcggtcgttt cccggcgccg acccggccgg
     1681 agcgcgccgc gctcacggcg ggcgcgcctg acggggccgt cacggccggt catgcctgag
     1741 gttttccgga cgggggtgtc cggacgctgg tacacgggtc atccgggggg agtggcccgt
     1801 gtgccggcac tccatcgccg gggtgaacat acatagcacg atgcgtgccg ggctgagcgg
     1861 actgccggtc atccagggct ggccgggacc cgcgccggtg tctgccgaag cctgcgtgcc
     1921 ttgccggtgt gcaggcttcg ccgcccggca ccggaacggc cacgcgccgg ccgcggcctg
     1981 aagcggcacg ggcttcagcg gaaaccgtcc tcgcggcgca ggccgtactt cttcagcttg
     2041 tcgtacagcg tcttgcgcgg cacgccgagc gcatcggcgg tggcgacgac gttgccgtcc
     2101 tggcgttcga gctcctgcac gatgacgacg cgctcgaagg ccgcgacctg atcgggcagc
     2161 gactgcgccg gtgcgccggc ggcgagcgcg gccggaccga gcaggtgacc gagcacgaag
     2221 cggtcggcgg cgttggcgag ttcgcggacg ttgccgggcc acggctcgct catcagcgcc
     2281 gccagcgtgt cgccggcgac ctcgggtggc tcgcgccggt agcgttcggc ggccttcagc
     2341 acgaaatgcc ggaacagcag cgggatgtcc tcgcgccgct cgcgcagcgg cgggatcgcc
     2401 agcttgacga cgttgagccg gtaatacagg tcctcgcgga acctgccctc gcgcgccgcg
     2461 acgcgcaggt cgaccttggt cgcggcgacg acgcggatgt cgagcgcgac gccgcggttc
     2521 gagccgagcc gctcgatcat gcggtcctgc agcgcgcgca gcaccttgac ctgcagcgcc
     2581 agcggcatcg attcgatctc gtcgaggaac accgtgccgc cgtgcgcgtg ttcgagcttg
     2641 ccgacgcgcc ggccgctggc gccggtgaag gcgccggcct cgtggccgaa cagctcggat
     2701 tcgaagatgg tctcgggaat cgcgccgcag ttgagcgcga cgaaggcctg cccgcgccgg
     2761 tggctgcgct ggtgcagcag acgcgcgacc acctccttgc cggcgccggt ctcgccctcg
     2821 atcagcacgt cggcgccggt atcggcgagg ttgtcgatcg ccgcgcgcag gcgtgcgatg
     2881 ccggggctgc gcccgagcag cagcgcgtcg ccctcgcggt gcgcggccag ctcgcgccgc
     2941 agccggcggt tctccagcgt cagcgcgcgc ttgtcgaggc cgcggcgcac gatgtcgagc
     3001 agcgcctcgg tgcggaaagg tttttccagg aagtcgtagg caccgtcgcg gatcgcctgc
     3061 accgccatcg cgatgtcacc gtggccggtg accagcacca ccggcagctc cggatcgagc
     3121 gcggcgacgg cgcgctgcag cgccagcccg tccatgcccg gcatgcgcac gtccgagacc
     3181 acgatgccgt cgaactccgg cgacagccgc gccagcgccg cggcggccga cgcgagcggc
     3241 tcggcggtga tgccttcgag ttccagcgtc tgcaccgcgg ccaggcgcac gtgcggttcg
     3301 tcatcgacga cgaggacggt cgggttcatg cggactccag cggctgcggg gccggcagcg
     3361 gcagttcgac ggtgaagcag gcgccgccgc cggcgaggtt ttcggcgcgg atcgtgccgc
     3421 cgaggttctg gacgatgccg tagctgatcg acagcccgag cccgaggccc tcgccgaccg
     3481 gcttggtcgt gaagaagggt tcgaacgcgc gttccagcgt tccggcgtcc atgccggcgc
     3541 ccgtgtcgcg aatccggagg acgccgtagc gccctggcgc cccgctgtcg ccctcggccg
     3601 gggcgaacgg ctcctggctg gtgttcagcg tgagcgtgcc gccatcgggc atggcctcca
     3661 gggcgttcag cagcacgttc aggaagacgt gctggaactg ctgacggtct accagtacat
     3721 gtggatcgtc tggcgccagc tgctccacga ccgtgatgcg cggcttgagc atcggcgcga
     3781 gcccgctcat cgcctcgcgc accagctggt tcaggctgac gcgtcgcggc tggagcacct
     3841 gcttgcggcc gaaggccagg agttgctgcg tcagccgggc cgcac
//
LOCUS       DAOQJH010000187         3884 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_14437_length_3884_cov_3.364064, whole genome shotgun sequence.
ACCESSION   DAOQJH010000187 DAOQJH010000000
VERSION     DAOQJH010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3884)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3884)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3884
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14437_length_3884_cov_3.364064"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1485
                     /locus_tag="PLQ18_05480"
     CDS             <1..1485
                     /locus_tag="PLQ18_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018506708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HNE01138.1"
                     /translation="TRILPATATRIGPGGDEVVPVAELAAGDEVRVRPGETIPADGSV
                     LDGASSVDESLLTGESLPQARTVGDAVIGGSVNVESPLRVRVERVGADSVLSSIVRLL
                     DRAQSEKPRIAALADRIAGHFVAVLLLVSLLVWAWWWQHEPARAFPIVLAILVVTCPC
                     ALGLATPAAITAATGALTRIGLLTTRGHALETLARATHVVFDKTGTLTIGRLRLVDTV
                     AVRAGLTAEEALELAAALECGSEHPVGQAIVDAARGTRRALDLRNTPGHGVEGTIDGR
                     VYRIGKPAAAAEPPQASDAEASTWVVLADAEGTLAWLRFADRLRPDAPETVGRLRAAG
                     LEVWLLSGDRPAAVAAIAAEAGIAHAEGGLLPADKLARIEALQQGGAVVAMVGDGIND
                     APVLAAASVSIAMGSGTQLAHASADMILLSSGLGHLVDGVTTARRTLAIIRQNLAWSI
                     GYNVLAVPAAAAGWIAPWLSAIGMSASSLLVVVNALRLRARRRA"
     gene            1498..1749
                     /gene="ccoS"
                     /locus_tag="PLQ18_05485"
     CDS             1498..1749
                     /gene="ccoS"
                     /locus_tag="PLQ18_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018869087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HNE01139.1"
                     /translation="MEILYLLIPLAIAIMLVAVWAFVWSVKSGQFDDLEGPAHRILMD
                     DDDPMIPRPEPSADKPSGPSRPSASPEASRPSAARDGDA"
     gene            1888..2868
                     /gene="birA"
                     /locus_tag="PLQ18_05490"
     CDS             1888..2868
                     /gene="birA"
                     /locus_tag="PLQ18_05490"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014777233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HNE01140.1"
                     /translation="MPARAAQLLERLADGGFRSGEVLAAELGISRAAVWKLVRGWQAR
                     GLDIQAVRGRGYRLARPLELLDAGLIQARLDETAAARLGPLEVLAETDSTNTRLAQLA
                     RSGLPAGAVCLAESQRAGRGRLGRQWISPYAANLYLSVLWRFALPPAALAGLSLAIGV
                     ALARSLERFGVGGHGLKWPNDLLWDARKLGGVLLEFGGESEGCSHVIIGVGVNVAMPE
                     SVGGTIDQPWIDLATVLGRTHVSRNRLAALLLSEIAIACTAFGESGLDSFQSDWQRWD
                     AVAGRPVRLLWPGHVVEGIARGIAADGELQLDTASGRRSFAIGELSLRPA"
     gene            2865..3620
                     /locus_tag="PLQ18_05495"
     CDS             2865..3620
                     /locus_tag="PLQ18_05495"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HNE01141.1"
                     /translation="MTGRLLIDAGNTRLKWRLDAEDGPRIAGCLVHRDRPLAEALAEL
                     PDALARPSAIWCGSVAGSHFDAALDAALRARWSLSPTFVQATAAAGGIVSAYAEPARM
                     GVDRWLAMIGARARTRRACCVIDAGTAVTVDALAADGRHLGGTILPGLQLMRTALYGN
                     TRRIPAGPVPATPSGLGRDTAACVAAGVRHAVVGGITGSVQSAGAGAGEGLHCLLTGG
                     DGAFLAPLLPFAVELVPDLVLDGLARLAGSAGA"
     gene            3682..3757
                     /locus_tag="PLQ18_05500"
     tRNA            3682..3757
                     /locus_tag="PLQ18_05500"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3715..3717,aa:Thr,seq:tgt)
BASE COUNT          488 a         1394 c         1405 g          597 t
ORIGIN      
        1 acccgcatcc tgccggcgac cgcgacccgc atcggtcccg gaggcgacga ggtcgtgccg
       61 gtggccgaac tcgcggccgg cgacgaggta cgcgtgcggc ccggcgagac gattccggcc
      121 gatggcagcg tgctcgacgg ggcgagcagc gtcgatgaat cgctgctgac cggcgagagc
      181 ctgccgcagg cccgcacggt cggcgatgcc gtgatcggcg gctcggtcaa cgtcgaaagc
      241 ccgctgcgcg tgcgcgtcga gcgcgtcggg gccgacagcg tgctgtcgtc gatcgtgcgc
      301 ctgctcgatc gcgcgcagag cgagaagccg cgcatcgccg cgcttgccga ccgcatcgcc
      361 gggcacttcg tcgccgtgct gctgctggtg tcgctgctgg tctgggcctg gtggtggcag
      421 cacgaaccgg cgcgcgcctt cccgatcgtg ctcgcgatcc tggtcgtgac ctgtccgtgc
      481 gcgctcggtc tggcgacacc ggccgcgatc accgccgcga ccggcgcgct gacgcgcatc
      541 ggcctgctga ccacgcgcgg ccacgcgctt gaaaccctgg cgcgcgcgac gcacgtggtg
      601 ttcgacaaga ccggcacgct gaccattggc cggctgcgcc tggtcgatac cgtggcggtg
      661 cgcgcggggc tcacggccga ggaggcgctc gaactcgcgg cagcgctcga atgcggctcc
      721 gaacaccctg tcgggcaggc gatcgtcgat gccgcgcgcg gcacgcggcg cgcgctcgat
      781 cttcgcaaca ccccgggcca cggcgtcgag ggcacgatcg acggccgcgt ctaccggatc
      841 ggcaagccgg ctgccgcagc cgagccgccg caggcaagcg atgccgaagc ctcgacctgg
      901 gttgtgctcg ccgatgccga aggaaccctc gcgtggctgc gcttcgccga ccgcctgcgt
      961 ccggatgccc ccgagacggt cgggcggctg cgggctgccg ggctcgaagt ctggctgctg
     1021 tccggcgacc ggccggcggc ggttgcggcc atcgccgccg aggccggcat cgcccacgcc
     1081 gaaggcgggc tgctgccggc ggacaagctc gcgcgcatcg aggcactgca gcagggcggg
     1141 gcggtggtgg cgatggtcgg cgacggcatc aacgatgcgc cggtgctcgc ggcggccagc
     1201 gtctcgatcg cgatgggttc gggcacgcag ctcgcgcatg cctcggccga catgatcctg
     1261 ctgtcctcgg ggctcggaca tctcgtcgac ggcgtcacca ccgcacggcg cacgctggcg
     1321 atcatccgcc agaacctcgc ctggtcgatc ggctacaacg tgctggccgt gccggccgcc
     1381 gccgccggct ggatcgcccc ctggctgtcg gcgatcggca tgtcggcgag ttcgctgctc
     1441 gtcgtcgtca atgcgctgcg cctgcgcgcg cgccgccggg cctgagggag cgccagcatg
     1501 gaaatcctgt acctgctgat cccgctcgcg atcgcaatca tgctggtcgc ggtgtgggcc
     1561 ttcgtctggt cggtgaagtc cggccagttc gatgacctcg aaggtccggc gcaccgcatc
     1621 ctgatggacg acgacgaccc gatgatcccg cgtcccgagc cgtccgccga caaaccttcc
     1681 ggaccctcca gaccttccgc ttccccggaa gcttcccggc cgtccgctgc gcgtgacggc
     1741 gatgcctgaa gtatcatccg accctcaccg cccaaggagt catgtccatg ttcacgttcg
     1801 agaacttttc cctggtgatc atcattgccc tgatcatcct gtcctggctg agcttcttct
     1861 cggtcatcct ctgacggcag gccggtcgtg ccggcacgcg ccgcgcagct gctggagcgc
     1921 ctcgccgatg gcggcttccg ttccggcgag gtgctggcag ccgaactcgg catcagccgg
     1981 gcggcggtct ggaagctcgt gcgcggctgg caggcgcgcg gcctcgacat ccaggccgtg
     2041 cgcgggcgcg gctaccggct ggcccgtccg ctcgaactgc tcgacgccgg gctgatccag
     2101 gcccggctcg acgagaccgc cgcggcccgc ctcgggccgc tcgaagtgct cgccgaaacc
     2161 gattcgacca acacccggct cgcgcagctg gcgcgcagcg gcctgccggc gggcgcggtc
     2221 tgtctggccg aaagccagcg cgccggccgc ggccggctcg gccggcagtg gatctcgccg
     2281 tacgcagcca acctgtacct gtcggtgctg tggcgcttcg cgctgccgcc cgccgcgctc
     2341 gccgggctgt cgctcgcgat cggcgtggca ctggcgcgtt cgctcgaacg cttcggcgtc
     2401 ggcggccacg gcctcaagtg gccgaatgac ctgctgtggg acgcccgcaa gctcggcggc
     2461 gtgctgctcg aattcggcgg cgagagcgaa ggctgcagcc acgtcatcat cggcgtcggc
     2521 gtgaacgtcg cgatgccgga gtccgtcggc ggcacgatcg accagccgtg gatcgacctg
     2581 gcgacagtgc tcgggcgcac gcatgtctcc cgcaaccggc ttgcggcgct gctgctgtcc
     2641 gagattgcca tcgcctgcac ggccttcggc gagtcggggc tcgacagctt ccagtccgac
     2701 tggcagcgct gggacgcggt cgccggccgg ccggtgcggc tgctgtggcc gggtcacgtc
     2761 gtcgagggca tcgcgcgcgg catcgcggcc gacggcgaac tgcagctcga taccgccagc
     2821 ggccggcgca gcttcgcgat cggcgaactg agcctgaggc cggcatgacg ggccggctgc
     2881 tgatcgatgc cggcaacacc cggctgaagt ggcgcctcga tgccgaagac gggccgcgga
     2941 tcgccggctg cctcgtgcat cgcgaccgcc cgctggccga ggcccttgcc gaactcccgg
     3001 acgcgctcgc acggccttcg gcgatctggt gcggcagcgt cgccggcagc cacttcgacg
     3061 ccgcgctgga tgctgcgctg cgggcacgct ggagcctgtc gccgaccttc gtgcaggcga
     3121 cggccgcggc cggcggcatc gtcagtgcct atgccgagcc ggcgcgcatg ggggtcgacc
     3181 gctggctggc catgatcggt gcgcgcgcac gcacacggcg ggcctgctgc gtgatcgatg
     3241 ccggcacggc cgtgaccgtc gatgcgctcg ccgcggacgg ccggcatctg ggcggcacga
     3301 tcctgcccgg cctgcagctg atgcgtaccg cgctgtacgg caacacccgc cgcatccccg
     3361 ccgggccggt gccggccacg ccctcaggtc tcggtcgcga cacggccgcc tgtgtggccg
     3421 ccggcgtgcg gcatgccgtc gtcggcggca tcaccggcag cgtccagagc gccggagccg
     3481 gggcgggcga gggtctgcac tgtctgctga ccggtggcga tggcgccttc ctcgcgccgc
     3541 tgctgccgtt cgcggtcgag ctcgtgccgg atctggtgct cgacgggctg gcgcggctgg
     3601 ccggttccgc aggcgcctga gcggtttgcg ggcgctcgcc ggcttcgtct acactgcgcg
     3661 ccccgtggcg ttgccgggca cgctggcgta gctcagtcgg tagagcagct gtcttgtaaa
     3721 cagcaggtcg tccgttcgat ccggaccgcc agcaccagat atccggaaga agtcgcagga
     3781 agtagtgttg acgggtccgg aagtgcacgg cagaatgcgc gtcctgtttt ggaggggttc
     3841 ccgagcggtc aaagggagca gactgtaaat ctgccggctc agcc
//
LOCUS       DAOQJH010000188         3881 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_14452_length_3881_cov_2.954522, whole genome shotgun sequence.
ACCESSION   DAOQJH010000188 DAOQJH010000000
VERSION     DAOQJH010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3881)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3881)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3881
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14452_length_3881_cov_2.954522"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..247
                     /locus_tag="PLQ18_05505"
     CDS             <1..247
                     /locus_tag="PLQ18_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019594987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hydrolase 1, exosortase A system-associated"
                     /protein_id="HNE01142.1"
                     /translation="LPARMAECLRRFRGPVLLVLSGDDLTAAEFRDCVQQSAQWQALL
                     AEARVTRRELAGANHTFSRRVWRDAVAGWTLEWLRTW"
     gene            241..1521
                     /locus_tag="PLQ18_05510"
     CDS             241..1521
                     /locus_tag="PLQ18_05510"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNE01143.1"
                     /translation="MVNPMRSPGAGGRRVLLIAFHYPPASESSGIQRTLKFSQYLPEH
                     GWTPAVLSAHPRVYDRVSSEQLAEVPAGMPVLRAFALDTARHLSIAGRYPRALALPDR
                     HVSWALGGIVQGLALIRRFRPDVLWSTYPIASAQLIGATLARLTGLPWVADLRDSMTE
                     DEYPRDPVQRASFLKLEKRVVTHAARVVFTTPGARAMYAARYPEVPPERWTVIENGFD
                     EENFTRAAGREPPPRSDRARVRLVHSGVLYPSERDPRPFFDALAALKARGVVDAARLC
                     VVLRYTGHDALHRSEIERRGIQDLIELAPPVPYEAALAEQLAADGLLLFQAANCNHQI
                     PAKLYEYLRAGRPILALTDAAGDTAATLRAAGIDSIVALDDAAAIEAALATFLDRIED
                     GSAVRIGRPQAERHGRRARTGELAALLDALVASR"
     gene            1550..2689
                     /locus_tag="PLQ18_05515"
     CDS             1550..2689
                     /locus_tag="PLQ18_05515"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HNE01144.1"
                     /translation="MALWGVVALLIGYGSLYPFRFRFPGLPLESLLQVPPLWTRPSDV
                     FGNMLLFVPWSLCGWWAGRGTAWRRAAPVLLGGLAWALLLQVLQLWLPERVASLGDVF
                     WNALGLAAGLLLATRLHDAPRTACIAILALWLLGLLLPLLPFWPAGLPLAWVRSLQAT
                     LSLPLPAALVWHVAGGWLSMRLLQGLGAGVRAAVGLVVGVLLLGPALPAAHPDPAQML
                     GLPLGVLCALYGRERADGLLIGLIAAVYTFDGLWPFRWRPLGMLSLQPFDSLLHGNML
                     NNLRHLCARLAIYAGVLWLIQQRCQRLPPLATLLVVGWVVSVEGAQAWLVGRYADIGE
                     LFWVLLAAGLVHLAGPLRPAAGAAPVSGPHGLDPLASTAGRSGEG"
     gene            complement(2726..>3881)
                     /locus_tag="PLQ18_05520"
     CDS             complement(2726..>3881)
                     /locus_tag="PLQ18_05520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HNE01145.1"
                     /translation="SNLVPVANTDELRSAMAAALPGDVLLLAPGDYPLQRTLYAERPG
                     EAGRPVRVLAERPGSVRIRVATTVGFELRAPYWWFEGLDIAGVCADHGDCEHAFHIVG
                     DARHTVLRNNRLHEFNAPVKINGSERDGRLQTPDDGRIDGNSLYNSGVRLTGNPVTLI
                     DAVAVNGWTVRANLIADFVKGGGDNISYGAFFKGAGSGNVFERNLVICEMNLHPPYGA
                     RVGLSFGGGGTGAPYCRDGRCAAEHFDGRIRNNLILHCNDVGVYLNRAARTEVAHNTL
                     YATLGMDARFPDTDAVFTNNLLDGRIKARDGGRWSGDGNLRLEAAGFAARFFAPQDAD
                     FRLRDGSGIVAAGVPLPQVPDDLCGHVRARLPDIGAIEYGLDACDPAGRP"
BASE COUNT          442 a         1398 c         1438 g          603 t
ORIGIN      
        1 gctgccggcg cgcatggccg agtgcctgcg ccgcttccgc gggccggtgc tgctggtgct
       61 gtccggcgac gacctgaccg cggccgaatt ccgcgactgc gtgcagcagt cggcgcagtg
      121 gcaggcgctg ctcgccgagg cgcgcgtcac gcggcgcgag ctggccgggg ccaatcacac
      181 cttttcccgg cgcgtgtggc gtgatgccgt ggccggctgg acgctggagt ggctgcgcac
      241 atggtgaatc cgatgcggtc tcccggagcg ggcgggcggc gcgtgctgct gatcgccttc
      301 cactatccac cggccagcga gagcagcggc atccagcgca cgctgaagtt ctcgcagtac
      361 ctgccggagc atggctggac gccggcggtg ctgagcgcgc atccgcgcgt ctacgaccgc
      421 gtcagcagcg agcagctcgc cgaggtcccc gccggcatgc cggtgctgcg ggccttcgcg
      481 ctcgacaccg cgcgccacct gtcgatcgcc ggccgctatc cgcgcgcgct ggcgctgccc
      541 gaccggcacg tgagctgggc gctcggcggc atcgtgcagg ggctggcgct gatccggcgc
      601 ttccggcccg acgtgctgtg gtcgacctac ccgatcgcca gcgcgcagct gatcggcgcg
      661 acgctggcgc gcctgaccgg cctgccgtgg gtcgccgacc tgcgcgattc gatgaccgag
      721 gacgagtacc cgcgcgatcc ggtgcagcgc gcgagcttcc tgaagctcga aaagcgtgtc
      781 gtcacgcacg ccgcgcgcgt ggtgttcacg acgccgggcg cacgcgcgat gtacgcggcg
      841 cgctatccgg aggtgccgcc cgaacgctgg acggtcatcg agaacggctt cgacgaggag
      901 aacttcacgc gcgccgccgg ccgcgagccg ccgccgcgca gcgaccgggc gcgcgtgcgg
      961 ctcgtgcaca gcggcgtgct gtacccgagc gagcgcgatc cgcggccgtt cttcgatgcg
     1021 ctcgccgcgc tgaaggcgcg cggcgtcgtc gatgccgcgc gcctctgcgt ggtgctgcgc
     1081 tacaccggcc acgatgcgct gcaccgcagc gagatcgagc gccgcggcat ccaggacctg
     1141 atcgaactgg cgccgcccgt gccctacgag gccgcgctcg ccgaacagct cgcggccgac
     1201 ggcctgctgc tgttccaggc cgccaactgc aatcaccaga ttccggccaa gctctacgaa
     1261 tacctgcgcg ccggccggcc gatcctggcg ctgaccgacg ccgccggcga taccgccgcg
     1321 acgctgcgcg ccgccggcat cgacagcatc gtcgcgctcg acgatgccgc tgcgatcgag
     1381 gccgcgcttg cgaccttcct cgatcgtatc gaggacggca gcgccgtgcg catcggacgc
     1441 ccgcaggccg agcgccacgg ccgccgtgcc cgcaccggcg aactcgccgc gctgctcgac
     1501 gcgctcgttg catcgcgctg agccgcgccg cccgtcgagc gcggcgcgca tggcgctgtg
     1561 gggcgtcgtc gcgctgctga tcggctacgg ctcgctgtat ccgttccgct tccgcttccc
     1621 gggcctgccg ctggaaagcc tgctgcaggt accgccgctg tggacgcgcc cgagcgacgt
     1681 gttcggcaac atgctgctgt tcgtgccgtg gtcgctgtgc ggctggtggg ccgggcgcgg
     1741 cacggcctgg cgccgcgccg cgccggtgct gctcggcggt ctggcctggg cgctgctgct
     1801 gcaggtgctg cagctctggc tgccggagcg cgtcgcctcg ctcggcgacg tgttctggaa
     1861 tgcgctcggg ctcgccgccg gcctgctgct ggccacgcgc ctgcacgatg cgccgcgcac
     1921 cgcctgcatc gcgatcctcg cgctctggct gctcggcctg ctgctgccgc tgctgccgtt
     1981 ctggccggcc ggcctgccgc tcgcctgggt gcgttcgctg caggcgacgc tgagcctgcc
     2041 gctgccggcg gcgctggtct ggcacgtcgc cgggggctgg ctgtcgatgc ggctgctgca
     2101 ggggctcggc gccggggtgc gggcggccgt gggcctggtc gtcggcgtgc tgctgctcgg
     2161 gccggcgctg cccgccgccc atcccgatcc ggcgcagatg ctcggcctgc cgctcggcgt
     2221 gctgtgcgcg ctgtatggtc gcgagcgcgc cgacggcctg ctgatcgggc tgatcgctgc
     2281 cgtctacacc ttcgacggcc tctggccgtt ccgctggcgg ccgctcggca tgctgtcgct
     2341 gcagccgttc gacagcctgc tgcacggcaa catgctgaac aacctgcggc atctctgtgc
     2401 gcggctggcg atctacgccg gcgtgctgtg gctgatccag cagcgctgcc agcgcctgcc
     2461 gccgctcgcc acgctgctgg tggtcggctg ggtcgtcagt gtcgagggcg cgcaggcctg
     2521 gctggtcggg cgctatgccg acatcggcga gctgttctgg gtgctgctcg ccgccggcct
     2581 cgtgcatctg gccggaccgc tgcgcccggc ggcgggcgca gcgccggtga gcgggccgca
     2641 cggcctggac ccgctcgcaa gcaccgccgg ccgcagcggc gagggctgag ccgcaggccg
     2701 tcgcacgctc cggattgtgc ctgccttacg gccgcccggc cggatcgcag gcgtcgaggc
     2761 cgtattcgat ggcgccgatg tcgggaagcc gggcgcggac gtggccgcag agatcgtccg
     2821 gaacctgcgg cagcggcacg ccggcggcaa cgatgccgct gccgtcgcgc agccggaagt
     2881 cggcgtcctg cggtgcgaag aagcgcgcgg cgaagccggc ggcttcgagc ctcaggttgc
     2941 cgtcgccgct ccagcgcccg ccgtcgcggg ccttgatgcg gccgtcgagc aggttgttcg
     3001 tgaacacggc atcggtgtcc gggaagcgcg cgtccatgcc gagcgtcgca tacagcgtgt
     3061 tgtgcgcgac ctcggtgcgc gcggcgcggt tcaggtacac gccgacatca ttgcagtgca
     3121 ggatcaggtt gttgcggatg cggccgtcga agtgttcggc tgcgcagcgc ccgtcgcggc
     3181 aatacggcgc cccggtgccg ccgccgccga aggacaggcc gacgcgcgcg ccgtagggcg
     3241 ggtgcaggtt catctcgcag atgaccagat tgcgttcgaa cacgttgccg ctgccggcac
     3301 ccttgaagaa ggcgccatag ctgatgttgt cgccgccgcc cttgacgaag tcggcgatca
     3361 ggttggcgcg caccgtccag ccgttgacgg cgaccgcgtc gatcagcgtc acggggttgc
     3421 cggtcaggcg cacgccggag ttgtagaggc tgttgccgtc gatgcggccg tcatcggggg
     3481 tctgcagccg gccgtcgcgc tcgctgccgt tgatcttcac cggcgcgttg aattcgtgca
     3541 ggcggttgtt gcgcagcacc gtgtggcggg catcgccgac gatgtggaag gcgtgttcgc
     3601 agtcgccgtg atcggcgcag acaccggcga tgtcgagacc ctcgaaccac cagtacggcg
     3661 cgcgcagttc gaagccgacc gtggtcgcga cgcggatgcg cacgctgccc gggcgctcgg
     3721 cgagcacgcg gaccggccgc ccggcctcgc ccggacgctc ggcgtacagc gtgcgctgca
     3781 gcgggtaatc gccgggcgcg agcagcagca cgtccccggg cagcgccgcg gccatcgcgc
     3841 tgcgcagttc gtcggtgttt gcgaccggca ccaggttgct g
//
LOCUS       DAOQJH010000189         3870 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_14522_length_3870_cov_3.631193, whole genome shotgun sequence.
ACCESSION   DAOQJH010000189 DAOQJH010000000
VERSION     DAOQJH010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3870)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3870)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3870
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14522_length_3870_cov_3.631193"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1202)
                     /gene="radA"
                     /locus_tag="PLQ18_05525"
     CDS             complement(<1..1202)
                     /gene="radA"
                     /locus_tag="PLQ18_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020394250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HNE01146.1"
                     /translation="MPPKTVRTVYVCDACGAVSPKWAGQCGECGAWNSLQETVAAPPA
                     ATAARGGRLAGYAGASETRVQNLAEVGTDEAARLATGIQELDRVLGGGLTVGSVVLIG
                     GDPGIGKSTLLLQTLAHLAAQHRALYVTGEESPQQVSLRAQRLGLPREPLRLLPETCI
                     ERVLAVAAAEQPRVMVIDSIQTAYTELLQSAPGAVAQVRESAAQLVRFAKRSGTALFL
                     VGHVTKEGTLAGPRVLEHMVDTVLYFEGDSGSRFRALRAVKNRFGPVNELGVFAMTET
                     GLKEVTNPSAIFLSRHPEPVAGSVVMVTREGTRPLLVEVQALVDDCHGGNPRRVTLGL
                     EQNRLAMLLAVLHRHAGIAMFDQDVYVNVVGGVRVQETASDLAVLMAALSSFRDRPLP
                     NDLIVFGEV"
     assembly_gap    1420..1519
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2084..3265)
                     /locus_tag="PLQ18_05530"
     CDS             complement(2084..3265)
                     /locus_tag="PLQ18_05530"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014197508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNE01147.1"
                     /translation="MRERYDVVIVGGAAVGSAAAYFLAADPAFAGASLLVVEKDASYR
                     EAATTLSAASIRHQFSTPENIRLSQFGTAFLREVGTALRVDDEVPNVGFREGGYLFLA
                     GPEGLTALETRHETQRALGADVRLLKPQLLAECFPWLATGDLAGASLGLSGEGWLDAW
                     SLLRALKRKARSLRAEYVEDCVVGLSRRGRRIESVRLAGGATVGCRWLLNCAGTGATS
                     LAALAGIELPVEARKRCVFHFQCATPISGSPLVVDPSGAWFRPEGPGWIAGIAPPPGA
                     DPVDPGFEVDWSQWEERLWPLLAARVPAFEAVRVKSAWAGHYDVNTLDCNAILGPHPG
                     LDNLLFANGFSGHGLQQAPAVGRALAEWVTTGASRSLDLSRFGWARVLAGQALRESEV
                     V"
     gene            3537..>3870
                     /locus_tag="PLQ18_05535"
     CDS             3537..>3870
                     /locus_tag="PLQ18_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006220787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNE01148.1"
                     /translation="MPVVAETPTSPADGAPIRPANAPPAPLAERLRRLRTARGWTLAQ
                     AAKACAVARSTLSKIENAQMSPTYELLLKLADGFGLGIGELLAADDAGPAHGGGRRSL
                     ARPGSGDGS"
BASE COUNT          601 a         1370 c         1325 g          474 t
ORIGIN      
        1 acttcgccga acacgatcag atcattcggc agcggccggt cgcggaagct cgacagtgcc
       61 gccatcagca cggcgaggtc cgaggccgtc tcctgcacgc gcacgccgcc gacgacgttg
      121 acgtaaacat cctgatcgaa catcgcgatg ccggcgtggc ggtgcagcac ggcgagcagc
      181 atcgccagcc ggttctgctc cagcccgagc gtcacgcgcc gcggattgcc gccgtggcag
      241 tcatcgacga gcgcctgcac ctcgaccagc agcggccgcg tgccctcgcg cgtgaccatg
      301 accaccgaac cggcgaccgg ctcggggtgg cgcgacagga agatcgccga cggattcgtg
      361 acttccttca ggccggtctc ggtcatcgcg aacacgccga gttcgttgac cggtccgaag
      421 cggttcttga ccgcgcgcag cgcccggaag cgcgagccgg aatcgccttc gaagtacagc
      481 accgtgtcga ccatgtgttc gagcacgcgc ggcccggcaa gcgtgccctc cttggtcacg
      541 tgcccgacca ggaacagcgc cgtgccgctg cgcttggcga agcgcacgag ctgcgccgcg
      601 ctctcgcgca cctgcgcgac cgcaccgggc gccgactgca gcaactcggt gtacgcggtc
      661 tggatcgagt cgatgaccat cacccgcggc tgctcggccg ccgccaccgc cagcacgcgt
      721 tcgatgcagg tctcgggcag caggcgcagc ggctcacgcg gcaggccgag gcgctgcgcg
      781 cgcaggctga cctgctgcgg cgattcctcg ccggtcacgt acagcgcccg gtgctgcgcg
      841 gccagatgcg cgagcgtctg cagcagcagc gtcgacttgc cgatgccggg atcgccgccg
      901 atcagcacca ccgagccgac cgtcagcccg ccgccgagca cgcggtcgag ctcctggatg
      961 ccggtcgcaa gccgcgcggc ctcgtcggtg ccgacctcgg cgaggttctg cacgcgggtc
     1021 tcactcgctc cggcatagcc ggcgagccgc ccgccgcgcg cggcggtggc ggccggcggc
     1081 gcggcgacgg tctcctgcag gctgttccac gccccgcact cgccgcactg cccggcccac
     1141 ttcggcgaca cggcaccaca ggcatcgcag acgtagacgg tgcggacggt tttcggcggc
     1201 atggcgactc ccgggggcag gtggggaaga tacggacgat cggggccggc cggccggtac
     1261 tcaagccatg ccctgcgctc gcagcaatgt tacgaatttt cgcagccgac gatgcgctgc
     1321 cgcccgatgg gcataccccg ggtggatcga cacaatgcat cacatccgga cctgatcacg
     1381 caggactgct ccgaaggacc ggagcccgca gcaggaagcn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnc cggcaggcac acggaagctt cagggaatcc cccgccggat
     1561 gcgggaccac ggaacggaca cggaagcgca acatcactgc gcgctgcgca gcgcccgccg
     1621 ggatgacgga cgcgacaacg gatgacagac ggacaccccg ccggagcggg cagcggcacc
     1681 ggaggccggc acggacaagt cactgacgat ccgccggatg cgggcggcag gcacggcagc
     1741 ggcagaggaa aacccgcacg gagcgcgggc accaggacgg tcgggatctt tcaggagaga
     1801 cgggcgtcag ggcgacgaag gatgcacgga ggcaccaggc cgggatggca aggtggaccg
     1861 ccgcacggaa gcgggcaccc gggatgggtg tcgaggtcca ggctgcttca cctttttcac
     1921 ctttaacggc tcatccggac gagccggcgc agggatagcc agggaccccc ggcggagccg
     1981 gtggcccacg gaagggccag gatgtattcg ggccggtcgt cgcaagacgg ccggcctttg
     2041 tctttcctgc gtgcaggacc cgggcgaccg gcctgccccg ccctcacacc acctcgctct
     2101 cgcgcagtgc ctgcccggcc agcacccgcg cccagccgaa acgcgacagg tcgaggctgc
     2161 gtgaggcgcc ggtcgtgacc cattcggcga gtgcgcggcc gactgccggc gcctgctgca
     2221 gaccgtggcc gctgaagccg ttggcgaaca gcaggttgtc gaggcccggg tgcgggccga
     2281 ggatggcgtt gcagtcgagc gtgttgacgt cgtaatgccc ggcccaggcg ctcttgacgc
     2341 gcaccgcctc gaaggccggc acgcgcgcgg cgagcagcgg ccacaggcgc tcctcccact
     2401 ggctccagtc gacctcgaag cccggatcga ccggatcggc cccgggcggc ggggcgatgc
     2461 cggcgatcca gcccggacct tcgggccgga accaggcgcc ggagggatcg accaccagcg
     2521 gactgccgct gatcggcgtc gcgcactgga aatggaacac gcagcgcttg cgcgcctcga
     2581 ccggcagctc gatgccggcg agggcggcga ggctggtggc gccggtgccg gcgcagttga
     2641 gcagccagcg gcagccgacc gtcgcgccgc cggccaggcg caccgattcg atgcgccggc
     2701 cgcgccggct caggccgacc acgcagtcct cgacgtactc ggcgcgcagc gagcgcgcct
     2761 tgcgcttcag tgcccgcagc aggctccagg catcgagcca gccttcgccg gacagaccga
     2821 gcgaggcacc ggcaagatcg ccggtcgcga gccacggaaa gcattcggcg agcagttgcg
     2881 gcttgagcag gcgcacgtcg gcgccgagcg cgcgctgcgt ctcgtgccgg gtttcgagcg
     2941 ccgtcagccc ttcgggtccg gcgaggaaca gatagccacc ctcgcggaaa ccgacgttcg
     3001 gcacctcgtc atcgacgcgc agcgccgtgc cgacctcgcg caggaaggcg gtgccgaact
     3061 gcgacagccg gatgttctcg ggcgtcgaga actggtggcg gatcgaggct gccgacagcg
     3121 tcgtcgccgc ctcgcggtag ctcgcgtcct tctcgaccac gagcagcgag gccccggcaa
     3181 aggccggatc ggcagccagg aaataggctg ccgcactgcc gaccgcggca ccgccgacga
     3241 tcaccacatc gtaccgctca cgcaggggct gcacgtctgc tgctctcctc ggcctcgttt
     3301 gcttccatcg gctgccggcg aagctccgcc ggcacggttc ccgtacccgc gcgcgcacgg
     3361 tcgcgcggcg actgggcggc ggcgcagtat gcgcagcgga ccgatcccgt cagttctccg
     3421 aaagaggtat gacagccgga cggggatcgc cgtttccgat cggaaacgcc gtgctacatt
     3481 ggaatcactt ttgtccgctc cggcaagtcc ggacggctgc ctgcggagca caccccatgc
     3541 ccgtcgtcgc cgaaaccccg acctcccctg ccgatggcgc cccgatccgg cccgcgaacg
     3601 cgccgccggc gccgctcgcc gagcgcctgc gccgcctgcg cacggcgcgc ggctggacgc
     3661 tggcgcaggc cgccaaagcc tgcgcggtgg cgcgctcgac gctctcgaag atcgagaacg
     3721 cgcagatgtc gccgacctac gagctgctgc tgaagctcgc cgacggcttc ggactcggca
     3781 tcggcgaact gctcgccgcc gacgacgccg gaccggcgca cggcggcggc cggcgcagcc
     3841 tcgcgcggcc cgggagcggg gacggcagtc
//
LOCUS       DAOQJH010000190         3865 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_14557_length_3865_cov_3.144619, whole genome shotgun sequence.
ACCESSION   DAOQJH010000190 DAOQJH010000000
VERSION     DAOQJH010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3865)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3865)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3865
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14557_length_3865_cov_3.144619"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1480
                     /locus_tag="PLQ18_05540"
     CDS             <1..1480
                     /locus_tag="PLQ18_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002727124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HNE01149.1"
                     /translation="RPPMLVRCVRPLSGRPRIVTHLLPRFQNGELRPNITRGSNHARY
                     VGPTQTLRLTTDAPLSYVLEHVPFLLDRPMHFVLGPDEILNADIAETAGGLLARTRDY
                     WRDWARSLTIPFEWQDEVIRAAVTLKLCAYEETGAVVAALTTSIPEAAHSQRNWDYRF
                     CWLRDAYFVIQAMNRLGVTTTMEAYIGYIMDIVGDVETQGLGPLFAITRSADLSERVA
                     PALAGYRGMGPVRIGNEAWLQVQNDVYGSVVLAATHVFFDRRLELADRHGLFEQLERI
                     GSRAVQVWDKPDAGLWELRTREHVHTFSAVMCWAACDRLAKIAGVIGRRERSAYWRER
                     ADAMHARICAEAWHEPGGYFASCFGGGDLDASLLLLNELDFLTADDPRFAATVDAIGT
                     SLRSGDFLYRYQIPDDFGRPETAFTVCAFWYVEALTALGRRDEARDLFGKLLARRNPL
                     GLLSEDLDWHTGEQWGNFPQTYSMVGLINCAMRLSIGWEEAF"
     gene            1584..1955
                     /locus_tag="PLQ18_05545"
     CDS             1584..1955
                     /locus_tag="PLQ18_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE01150.1"
                     /translation="MHAADHPAAAWAARTVFDPVQIDCTTAVMLKILDGKCRMFEAEK
                     RAVAAVYAIVHKQPGRLLDAGVHALIRQAQAAPADAALRERVYEQRVLAETAISRPVM
                     KAYKAWLRAEGVIGGDGGAGD"
     gene            2180..2896
                     /locus_tag="PLQ18_05550"
     CDS             2180..2896
                     /locus_tag="PLQ18_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018952869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04283 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HNE01151.1"
                     /translation="MRGAAPWLSIIVPVLDDAPALDVLLERLRPLRTQGVELIVVDGG
                     SRDDSVALACARSTCVLTAPAGRARQMNAGAALARGQALLFLHADSVPPDSVDALIGA
                     ALGHGAHWGRFDVCLEGRDRRLGLVARLMNLRSRLSGIATGDQAIFVRADSFVQVGGY
                     PEQALMEDIELSRRLKRLGRPQCLAGPVRSSGRRWDEHGFWRTVTLMWWLRLAYRLGL
                     APDRLARWYYGRGPRGTAGA"
     gene            2893..3528
                     /locus_tag="PLQ18_05555"
     CDS             2893..3528
                     /locus_tag="PLQ18_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04282 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HNE01152.1"
                     /translation="MTAAGLALRLAVFAKAPLPGWAKTRLIPLLGAEGAARLAGTLIA
                     RTLALAEAVRPQALTLWCAPSAGHPYFRALAAGPSPPRLCTQTGADLGARMHAAFTDE
                     LARGPTLLIGTDCPALTAGHLHAAAEALLGTVAGPAADAVFLPAEDGGYVLVGLRTPQ
                     PRLFEDLPWSTPALMDATRARLRQAGLRGAEPATLWDLDRPADYRRWLKEG"
     gene            3652..>3865
                     /locus_tag="PLQ18_05560"
     CDS             3652..>3865
                     /locus_tag="PLQ18_05560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE01153.1"
                     /translation="MPATPSSTAAVSALGPEDGLLYGYWLRPGLLAQRIGLAELDEAL
                     ATETGTVWLHFNLSNVRARRWLAQSER"
BASE COUNT          499 a         1436 c         1374 g          556 t
ORIGIN      
        1 ccggccgccg atgctcgtgc gctgcgtgcg gccgctgtcc gggcggccgc gcatcgtcac
       61 gcacctgctg ccgcgcttcc agaacggcga gctgcgcccg aacatcaccc gcggcagcaa
      121 ccacgcgcgc tacgtcgggc cgacgcagac gctgcgcctg accaccgacg caccgctgtc
      181 ctacgtgctc gaacacgtgc ccttcctgct cgaccggccg atgcacttcg tgctcgggcc
      241 ggacgagatc ctgaacgccg acatcgccga gaccgccggc ggcctgctgg cgcgcacgcg
      301 cgactactgg cgcgactggg cgcgctcgct gacgatcccg ttcgaatggc aggacgaggt
      361 gatccgcgcc gcggtgacgc tgaagctctg cgcctacgag gaaaccggcg ccgtggtcgc
      421 ggcgctgacc acctcgatcc cggaggccgc gcattcgcag cgcaactggg attaccgctt
      481 ctgctggctg cgcgacgcct acttcgtgat ccaggcgatg aaccgcctcg gcgtcacgac
      541 gacgatggag gcctacatcg gctacatcat ggacatcgtc ggcgatgtcg agacccaggg
      601 tctcggcccg ctgttcgcga tcacgcgcag cgccgatctc tccgagcgcg tcgcgccggc
      661 gctcgccggc taccgcggca tggggccggt gcgcatcggc aacgaggcct ggctgcaggt
      721 gcagaacgat gtctacggca gcgtcgtgct cgccgcgacc cacgtgttct tcgaccgccg
      781 gctcgaactc gccgaccgtc acggcctgtt cgaacagctc gaacgcatcg gctcgcgcgc
      841 cgtgcaggtc tgggacaagc ccgatgccgg gctctgggaa ctgcgcacgc gcgagcacgt
      901 gcacacgttc tcggcggtga tgtgctgggc ggcctgcgac cggctcgcca agatcgccgg
      961 cgtgatcggc cggcgcgaac gctcggccta ctggcgcgag cgcgccgatg cgatgcacgc
     1021 gcgcatctgc gccgaggcct ggcacgagcc gggcggctac ttcgcgtcct gcttcggcgg
     1081 cggggacctc gatgcctcgc tgctgctgct gaacgaactc gacttcctca cggctgacga
     1141 tccgcgcttc gcggccaccg tcgatgcgat cggcaccagc ctgcgctccg gcgacttcct
     1201 gtaccgctac cagattccgg acgacttcgg ccggcccgag accgcgttca cggtctgcgc
     1261 gttctggtac gtcgaggcgc tgaccgcgct cgggcgccgc gacgaggcgc gcgacctgtt
     1321 cggcaagctg ctcgcgcggc gcaaccccct cggcctgctg tccgaggacc tcgactggca
     1381 caccggcgag cagtggggca acttcccgca gacctactcg atggtcgggc tgatcaactg
     1441 cgcgatgcgg ctgtcgatcg gctgggaaga agccttctga gcccgccctg cggcagcggc
     1501 cgccgctgaa ggcgcgccgc gcttgcagcc ggcgcggcgc tggcgtctgc tggtcttccc
     1561 gatcgccgac ccggagccgc gccatgcacg ctgccgatca tcctgccgcc gcctgggcgg
     1621 cccgcaccgt cttcgatccc gtgcagatcg actgcacgac ggcggtgatg ctgaagatcc
     1681 tcgacggcaa gtgccgcatg ttcgaggccg agaagcgcgc ggtcgcggcg gtctacgcca
     1741 tcgtccacaa gcagccgggg cggctgctcg atgccggcgt gcatgcgctg atccgtcagg
     1801 cgcaggcggc accggcggat gccgcgctgc gggagcgcgt ctacgaacag cgcgtgctcg
     1861 ccgagaccgc gatctcgcgg ccggtcatga aggcctacaa ggcctggctg cgcgccgaag
     1921 gcgtgatcgg cggcgacggg ggcgctggcg actgacccac gccgaccgcc gcgcgcaccc
     1981 gatgcgacct cgatgtgacc tcgatgcgat cacaacgatg gtggcgcggc gcagtgggcg
     2041 ggatcggccg tgctatacgt ccccggcatg cccgtcgatc gagtcgcccg atgatgcccg
     2101 tgcccccgcc atccgtccgc accggagcgc acccgtgagt gccgtcccgg attccgccgg
     2161 acacccggcc gcgtcgatcc tgcgcggtgc cgcgccgtgg ctgtcgatca tcgtgccggt
     2221 gctcgacgat gcgccggccc tcgatgtgct gctggagcgg ctcaggccgc tgcgcacgca
     2281 gggggtcgag ctgatcgtcg tcgatggcgg cagccgcgat gattcggtgg cgctcgcctg
     2341 cgcgcgcagc acctgcgtgc tcacggcgcc tgccgggcgc gcgcgacaga tgaacgccgg
     2401 cgcggcgctg gcacgcggtc aggcgctgct gttcctgcac gccgacagcg tgccgcccga
     2461 cagcgtcgat gcgctgatcg gcgccgcgct cggccacggc gcgcactggg ggcgcttcga
     2521 cgtatgcctc gaaggccgcg accgccggct cggactggtc gcgaggctga tgaacctgcg
     2581 ctcgcgcctg agcggcatcg ccaccggcga ccaggcgatc ttcgtgcgcg ccgacagctt
     2641 cgtgcaggtc ggcggctatc cggagcaggc gctgatggag gacatcgagc tgagccggcg
     2701 cctcaagcgc ctcggccggc cgcagtgcct ggccggaccg gtgcgcagct ccggccggcg
     2761 ctgggacgag cacggcttct ggcgcaccgt gacgctgatg tggtggctgc ggctggccta
     2821 ccggctcggt ctggcgccgg accggctggc gcgctggtac tacggacggg ggccgcgggg
     2881 cacggccggc gcatgacggc ggccgggctg gcgctgcgcc tggccgtctt cgccaaggca
     2941 ccgctgccgg gctgggccaa gacgcggctg attccgctgc tcggcgccga aggcgcggcg
     3001 cggctcgccg gaaccctgat cgcacgcacg ctcgcgctcg ccgaagccgt gcggccgcag
     3061 gcgctgacgc tctggtgcgc accgtcggcc gggcatccgt acttccgcgc gctggcggcc
     3121 gggccaagtc cgccgaggct ctgcacgcag accggcgccg atctcggcgc gcgcatgcac
     3181 gcggccttca ccgatgaact cgcccgcggt ccgacgctgc tgatcggcac cgactgcccg
     3241 gcgctcacgg ccgggcacct gcacgccgcg gccgaggcgc tgctcgggac cgtcgccggt
     3301 ccggcggccg atgccgtgtt cctgccggcc gaggatggcg gttacgtgct ggtcgggctg
     3361 cgcacgccgc agccgcggct cttcgaagac ctgccgtgga gcacgccggc gctgatggac
     3421 gcgacgcgcg cgcgcctgcg gcaggccggc ctgcgcgggg ccgagccggc gacgctgtgg
     3481 gacctcgacc ggcccgcaga ctaccggcgc tggctgaagg aaggctgaag cgcccgggcg
     3541 ccgatgatgc acctgcacaa tgacggcgct gcggatcggt tacgatgccg caaaccccgg
     3601 ccgccatccg ccggccggtc ctgccgcctc cagcttcgga ccctccctcc cgtgccagcc
     3661 acgccgtctt cgactgccgc cgtttccgcc ctcggtcccg aggacggcct gctgtacggc
     3721 tactggctgc gtccggggct gctcgcgcag cgcatcggac tcgccgagct cgacgaggcg
     3781 ctcgccaccg agaccggcac ggtctggctg cacttcaacc tgagcaacgt ccgcgcccgg
     3841 cgctggctcg cgcagtcgga gcgcg
//
LOCUS       DAOQJH010000191         3864 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_14564_length_3864_cov_4.214229, whole genome shotgun sequence.
ACCESSION   DAOQJH010000191 DAOQJH010000000
VERSION     DAOQJH010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3864)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3864)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3864
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14564_length_3864_cov_4.214229"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            218..898
                     /gene="rpe"
                     /locus_tag="PLQ18_05565"
     CDS             218..898
                     /gene="rpe"
                     /locus_tag="PLQ18_05565"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002684226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HNE01154.1"
                     /translation="MPDFLIAPSILSADFARLGAEVDAVLAAGADVVHFDVMDNHYVP
                     NLTVGPMVCKALRQYGVTAPIDVHLMVEPVDALVPMFAEAGASWITFHPEASRHVDRT
                     LQLIRSCGCKAGLVFNPATPLDVMQYVMDKLDIVLLMSVNPGFGGQSFIPATLGKLAE
                     ARARIAASGLPIRLEIDGGVKLDNIGEIARAGADTFVAGSAIFSTPDYAATIAALRAE
                     LARAAEEA"
     gene            898..1569
                     /locus_tag="PLQ18_05570"
     CDS             898..1569
                     /locus_tag="PLQ18_05570"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006746914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HNE01155.1"
                     /translation="MFSHVDAILFDLDGTLIDSVPDIAVAIDQLMDTLDLPRRGEAKV
                     RQWVGNGSTNLIRRALSDSMDGEADPELMDRAKPLYRRFYGAQVCVHSRLYPGVVEGL
                     DKLAKRKRFKLACVTNKPADLAAPLLERIGIAHHFHALVGGECTPTVKPAPECLLLAA
                     SRIGVHISRCLMVGDSRNDIGAARNAGCPVVAVPYGYNHGRDIREFAPDIVIDSLSEL
                     SGLID"
     gene            1730..3208
                     /gene="trpE"
                     /locus_tag="PLQ18_05575"
     CDS             1730..3208
                     /gene="trpE"
                     /locus_tag="PLQ18_05575"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006474347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HNE01156.1"
                     /translation="MNASEFAALAAEGYNRVPVVREVLADLDTPLSTYLKLADAPYSY
                     LLESVHGGEKWGRYSIIGLPARTVLRVRGHEVTVERGAEVVERAHVEDPLAWIEVFQR
                     RYRVPDNREGLPRFAGGLVGYFGYDTIRYVEPRLAHCPNPDPIGAPDILLMVSEDVVV
                     FDNLSGKLYVIAQVDPLAERAWERAQAKLDAVVERLRSGSTAYAHGHGAPLAEQDFVS
                     GFTQAGFEAAVERIKDYILAGDCMQVVPSQRLSAPYAGAPLDLYRALRSLNPSPYMYF
                     LNLGDCHIVGSSPEILARLEDGTVTVRPIAGTRKRGRDEAEDRALEADLLADPKEIAE
                     HLMLIDLGRNDAGRVCTTGSVRVTEQMVIERYSHVMHIVSNVSGRLKDGLTAMDVLRA
                     TFPAGTVSGAPKIRAMEIIDEVEPVKRGIYAGAVGYLSWSGNMDTAIAIRTAVIKDGV
                     LHVQAGAGIVADSVPASEWEETLNKGRAIFRAAALAGRGFGG"
     gene            3214..3798
                     /locus_tag="PLQ18_05580"
     CDS             3214..3798
                     /locus_tag="PLQ18_05580"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007230992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HNE01157.1"
                     /translation="MLLMIDNYDSFTYNLVQYFGELGADVQVFRNDGITPDEIERLAP
                     ARIVLSPGPCTPNEAGISMATIERFAGRIPILGVCLGHQSIGQVFGGRIVRAKQVMHG
                     KVSPVHHSGQGVFAGLPSPFTVTRYHSLVIEQATLPECLEVTAWTAGPDGSIDEIMGV
                     RHRTLAVEGVQFHPESILTEHGHALLANFLNAAA"
BASE COUNT          580 a         1388 c         1308 g          588 t
ORIGIN      
        1 agcgcgccac cgagcgcacg cacgcgatcc gctcggccta cgaacgcatc cgcagcgcac
       61 gcggcttcgg ctgacggcac acccgggccg ccgctgcggg attcccgtgt cgcagtgcaa
      121 cgtcgctgcc cgcgccgcgg ccggatcggg tacagtgccg cctcccctgc tcaagcggtt
      181 cgccgggctg cgctagcccg gtgcggagtc attggtcatg ccggatttcc tgatcgctcc
      241 atcgatcctg tcggccgatt tcgcgcggct cggcgccgag gtcgatgcgg tgctcgcggc
      301 cggagcggac gtcgtccact tcgacgtgat ggacaaccac tacgtcccga acctgaccgt
      361 cgggccgatg gtgtgcaagg cgctgcgcca gtacggcgtg acggcgccga tcgacgtgca
      421 cctgatggtc gagccggtcg atgcgctggt gccgatgttc gccgaggccg gcgcgagctg
      481 gatcaccttc caccccgagg ccagccgcca cgtcgaccgc acgctgcagc tgatccgctc
      541 ctgcggctgc aaggccgggc tggtgttcaa cccggcgacc ccgctcgacg tgatgcagta
      601 cgtgatggac aagctcgaca tcgtgctgct gatgtcggtg aaccccggct tcggcggcca
      661 gagcttcatt ccggcgaccc tcggcaagct cgccgaggcg cgcgcgcgca tcgcggcctc
      721 cgggctgccg atccggctgg agatcgacgg cggcgtcaag ctcgacaaca tcggtgaaat
      781 cgcccgtgcc ggggccgaca ccttcgttgc cggctccgcg atcttcagca cgccggacta
      841 cgctgcgacc atcgctgcgc tgcgggccga actggcccgt gcggcggagg aggcctgatg
      901 ttctcgcacg tggatgccat cctgttcgac ctcgacggca cgctgatcga cagcgtgccc
      961 gacatcgccg ttgccatcga ccagctgatg gacacgctcg acctgccgcg ccgcggcgag
     1021 gccaaggtgc gccagtgggt cggcaacggc tcgaccaacc tgatccgccg ggcactcagc
     1081 gactcgatgg acggcgaggc cgatccggaa ctcatggatc gcgcgaagcc gctgtaccgg
     1141 cgcttctacg gcgcgcaggt ctgcgtgcac agccgcctgt atccgggcgt ggtcgagggg
     1201 ctcgacaagc tcgccaagcg caagcgcttc aagctcgcct gcgtcaccaa caagccggcg
     1261 gatctcgccg cgccgctgct ggagcgcatc ggcatcgcgc atcacttcca tgcgctggtc
     1321 ggcggcgaat gcacgccgac ggtcaagccc gcgcccgaat gcctgctgct cgcggcctcg
     1381 cgcatcggcg tgcacatctc gcgctgcctg atggtcggcg attcgcgcaa cgacatcggc
     1441 gcggcgcgca acgccggctg cccggtggtc gccgtgccct acggctacaa ccacggccgc
     1501 gacatccgcg agttcgcgcc cgacatcgtc atcgacagcc tctccgagct gtcggggctg
     1561 atcgactgaa gcgcaggaaa gccggcaccc gacgatgatc ccgaacgaaa cccagcccat
     1621 ggacctgcga tggcggtggc ggcactgatc ccggttccgc cgctgcaacc gtcgtccgcc
     1681 cgtgcgcggg ccacggtacc gatcgccgtc tttcccgcag ggtttcccga tgaatgcctc
     1741 tgaattcgcc gccctcgccg ctgagggcta caaccgcgtg ccggtcgtgc gcgaggtgct
     1801 cgccgacctc gacacgccgc tgtcgaccta cctgaagctc gccgatgcgc cgtacagcta
     1861 tctgctggaa tccgtgcacg gcggcgagaa atggggccgc tactcgatca tcggcctgcc
     1921 ggcgcgcacg gtgctgcgcg tgcgcggcca cgaggtgacg gtcgaacgcg gcgccgaggt
     1981 ggtcgagcgt gcgcacgtcg aggacccgct cgcgtggatc gaggtcttcc agcgccgcta
     2041 ccgcgtgccc gacaaccgcg agggtctgcc gcgcttcgcc ggcgggctgg tcggctactt
     2101 cggctacgac acgatccgct acgtcgagcc ccgcctcgcg cactgcccga atcccgatcc
     2161 gatcggcgcg cccgacatcc tgctgatggt gtccgaggac gtggtcgtct tcgacaacct
     2221 ctccggcaag ctgtacgtga tcgcgcaggt cgacccgctc gccgagcgcg cctgggagcg
     2281 cgcccaggcg aagcttgatg ccgtcgtcga gcgcctgcgc agcggctcga ccgcgtatgc
     2341 gcacggccat ggcgcgccgc tcgccgagca ggatttcgtc tccggcttca cgcaggccgg
     2401 cttcgaggcc gcggtcgagc gcatcaagga ctacatcctc gccggcgact gcatgcaggt
     2461 cgtgccctcg cagcggctgt cggcgccgta tgccggcgcg ccgctcgatc tgtaccgggc
     2521 gctcaggagc ctcaatccgt cgccgtacat gtacttcctg aacctcggcg actgccacat
     2581 cgtcggctcc agccccgaga tcctcgcgcg cctcgaagac ggtacggtca cggtccgccc
     2641 gatcgccggc acgcgcaagc gcggccgcga cgaggccgag gaccgcgcgc tcgaagcgga
     2701 tctgctcgcc gatccgaagg agatcgccga gcacctgatg ctgatcgacc tcggccgcaa
     2761 cgatgccggg cgcgtctgca cgaccggcag cgtgcgcgtc accgagcaga tggtcatcga
     2821 gcgctactcg cacgtgatgc acatcgtctc gaacgtcagc ggccggctga aggacgggct
     2881 gacggcgatg gacgtgctgc gcgcgacctt cccggccggc acggtcagcg gcgcgccgaa
     2941 gatccgggcg atggagatca tcgacgaggt cgagccggtc aagcgcggca tctacgccgg
     3001 cgcggtcggc tatctgtcct ggagcggcaa catggacacg gcgatcgcga tccgcaccgc
     3061 ggtgatcaag gacggcgtgc tgcacgtgca ggccggggcc ggcatcgtcg ccgactcggt
     3121 gccggcgagc gaatgggagg aaaccctgaa caagggccgc gcgatcttcc gcgccgccgc
     3181 actcgccggc cgcggcttcg gaggctgacg atcatgctgc tgatgatcga taactacgac
     3241 tcgttcacgt acaacctcgt gcagtacttc ggcgaactcg gggccgacgt gcaggtgttc
     3301 cgcaacgacg gcatcacgcc cgacgagatc gagcggctgg cgccggcgcg catcgtgctg
     3361 tcaccggggc cgtgcacgcc gaacgaggcc ggcatctcga tggcgacgat cgaacgcttc
     3421 gccgggcgca tcccgatcct cggcgtctgc ctcggccacc agagcatcgg ccaggtgttc
     3481 ggcgggcgca tcgtgcgcgc gaagcaggtc atgcacggca aggtctcgcc ggtgcatcac
     3541 agcggccagg gcgtgttcgc cggtctgccg agcccgttca cggtcacgcg ctaccactcg
     3601 ctggtcatcg agcaggcgac gctgcccgag tgcctggagg tcaccgcgtg gacggccggc
     3661 cctgacgggt cgatcgacga gatcatgggc gtgcgccacc ggacgctggc ggtcgagggc
     3721 gtgcagttcc acccggaatc gatcctgacc gagcacggcc acgccctgct cgcgaacttc
     3781 ctgaacgcag cggcctgagg ccgcctgcgc ggcggctccg gcgtcgggcg gcgccggggc
     3841 aggccgcgcg ccagcttccc gttt
//
LOCUS       DAOQJH010000192         3863 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_14570_length_3863_cov_4.858456, whole genome shotgun sequence.
ACCESSION   DAOQJH010000192 DAOQJH010000000
VERSION     DAOQJH010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3863)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3863)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3863
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14570_length_3863_cov_4.858456"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..572)
                     /locus_tag="PLQ18_05585"
     CDS             complement(<1..572)
                     /locus_tag="PLQ18_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006786599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HNE01158.1"
                     /translation="MYGLDQPVLRTDISGMPLEWIDYQEAVRLHCAGHVAFTAGTLLY
                     TLRGGCNAVTGRRSLIPVHAVLATFGGRYASLRLRPGYVPPLSNPALFRRDFNVCLYC
                     GDSFPVRELSRDHVTPLVQGGRDRWSNVVTACRRCNHLKGGRTPEQAHMPLLAVPFAP
                     THAEYVYLQGRRILADQMEFLRAHFPRSSPL"
     gene            910..2034
                     /locus_tag="PLQ18_05590"
     CDS             910..2034
                     /locus_tag="PLQ18_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017942676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HNE01159.1"
                     /translation="MTLRLVVPKETWTGERRVALDPSVAERFQRLGAEVQIERGAGCG
                     SHFGDAQYEKGAKLVDDIVAALAAADVTVRVQPPSLEEVEQLRDGSVLIGFLAPHRNA
                     EVIRRLRDKKITSFAFELLPRISRAQSMDALSSQAAIAGYKVALMAASLASFFFPMLT
                     TAAGTIRPAKVVVVGAGVAGLQAIATCKRLGAMVEAYDIRAAAKEQVESLGAKLIDTG
                     VDASGQGGYARELTKEEIARQAEVLARHIAAASAVITTAAIPGRPAPKIVTKAMVEAM
                     KPGAVIVDLAAETGGNCELTEPGQTIEHNDVTIHGPLNVPSLIPVHASETYAKNVFNF
                     LSPFVKNGELALDWNDEVIARSCLTHDGQIRHEPTRKLVG"
     gene            2040..2324
                     /locus_tag="PLQ18_05595"
     CDS             2040..2324
                     /locus_tag="PLQ18_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006914082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HNE01160.1"
                     /translation="MIEGFAALYIFMLAAFTGYEIIGRVPVILHTPLMSGSNFVHGIV
                     VVGAMVALGHAQTGLEQLLGFIGVVLAAGNAVGGYVVTERMLEMFKSSKE"
     gene            2331..3701
                     /locus_tag="PLQ18_05600"
     CDS             2331..3701
                     /locus_tag="PLQ18_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020395521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HNE01161.1"
                     /translation="MAGLIQIVYFAAAALFILGLKRMSSPKTARSGIVWAGAGMALAT
                     LVTFLYPGLSNYLLILVAIGVGSYAAWVSGKKVAMTDMPQMIAIYNGMGGGAAACIAA
                     VELLKGGSHGAVHALLGVLGALIGAVSFSGSVVAWAKLDGRMKKAIRFPRQKLVNLGV
                     FVLALLFGLIVMGASQPGAFSIIAFFLLALAFGAMMALPIGGADMPVVISLFNAFTGL
                     AVAFEGYVMQNAAMIIAGTVVGAAGSLLTQLMAKAMNRPISNVLFSNFGGGSGATAEE
                     EVKGSMKASEPQDAAVMMAYAAKVIIVPGYGMAVAQAQHKVWELAELLEENSVEVKFA
                     IHPVAGRMPGHMNVLLAEAGVPYDKIFDLDEINNEFASTDVVLVIGANDVVNPVAKSD
                     PNSPIYGMPVLNADQARNVLVIKRGQGAGFSGIENHLFYLDNTRMVYGDAQKVLAELI
                     GFVKTL"
BASE COUNT          616 a         1304 c         1324 g          619 t
ORIGIN      
        1 agcgggctgc tgcgcgggaa gtgcgcgcgc aggaattcca tctggtcggc gaggatgcgc
       61 cggccctgca gatagacgta ttcggcgtgc gtcggcgcga acggcacggc gagcagcggc
      121 atgtgcgcct gctcgggcgt gcgcccgccc ttcaggtgat tgcagcgccg gcaggcggtg
      181 acgacgttgc tccagcgatc gcgcccgccc tgcaccagcg gcgtcacgtg gtcgcgcgac
      241 agttcacgca ccggaaagct gtcgccgcag tacaggcaga cgttgaagtc gcgccggaac
      301 agcgccgggt tcgacagcgg cggcacgtaa ccggggcgca ggcgcaggct ggcgtagcgc
      361 ccgccgaagg tcgcgagcac ggcgtggacc gggatcaggc tgcgccggcc ggtgacggcg
      421 ttgcagccgc cgcgcagcgt gtacagcagc gtgccggccg tgaaggcgac atgcccggcg
      481 cagtgcaggc gcacggcttc ctggtagtcg atccactcca gcggcatgcc ggagatgtcg
      541 gtgcgcagca ccggctgatc cagaccgtac atcatccctc ccgtcgtgca gacgtggccg
      601 cccgtgtcgc ggcggcggat gaaggcacgg tgacggcggc gatctgcgga tgcgcacgca
      661 cggcaccgtc cggccttttg ggccagccgc ctagatacac cagtcgacga cgcaggggta
      721 gtgcgggcac gactttcgca gtgcaaaagc catcggatac catatccgga ccgattgcag
      781 acgttttaga gcaaagactc tagtctgccg cccctccggc acgcccggag cgggccatcc
      841 gccttgaacc cccggcactc gtccgtcccg ctctgcgggc cggtgtgttg ccgtctggag
      901 aacgcttaca tgaccttacg tctggtggtc ccgaaagaga cctggacggg cgagcgccgc
      961 gttgcgctcg accccagcgt cgccgagcgc ttccagcgcc tcggcgccga agtgcagatc
     1021 gagcgcggcg ccggctgcgg ctcgcatttt ggcgatgcgc aatacgaaaa gggcgcgaag
     1081 ctcgtcgacg acatcgtcgc ggcgctggcc gcggccgatg tcaccgtgcg cgtgcagccg
     1141 ccgagcctcg aagaggtcga gcagctgcgc gacggctcgg tgctgatcgg cttcctcgcc
     1201 ccgcaccgca acgccgaggt catccggcgc ctgcgcgaca agaagatcac gagcttcgcg
     1261 ttcgagctgc tgccgcgcat ctcgcgcgcg cagtcgatgg atgcgctgtc ctcgcaggcg
     1321 gcgatcgccg gctacaaggt cgcgctgatg gccgcgagcc tcgcgagctt cttcttcccg
     1381 atgctgacca cggccgccgg caccatccgc ccggccaagg tcgtcgtcgt cggtgccggc
     1441 gtcgccggcc tgcaggcgat cgcgacctgc aagcgcctcg gcgcgatggt cgaggcctac
     1501 gacatccgcg ctgcggccaa ggagcaggtc gagtcgctcg gtgccaagct gatcgacacc
     1561 ggcgtcgatg cctccggtca gggcggctat gcccgcgagc tgaccaagga ggaaatcgcc
     1621 cgccaggccg aggtgctcgc gcgccacatc gccgctgcct cagcggtgat caccaccgcc
     1681 gcgatcccgg gccgtccggc gccgaagatc gtcacgaagg cgatggtcga ggcgatgaag
     1741 cccggggccg tcatcgtcga tctcgccgcc gaaaccggcg gcaactgcga gctgaccgag
     1801 cccggccaga ccatcgagca caacgacgtg accatccacg gtccgctgaa cgtgccgagc
     1861 ctgatcccgg tgcacgcgag cgagacctac gcgaagaacg tcttcaattt cctgtccccg
     1921 ttcgtgaaga acggcgagct cgcgctcgac tggaacgacg aggtcatcgc caggagctgc
     1981 ctcacgcacg acggccagat ccgtcacgaa ccgacccgca agctggtggg gtgagcgtca
     2041 tgatcgaagg tttcgccgcg ctgtacatct tcatgctcgc cgccttcacc ggctacgaga
     2101 tcatcggccg cgtgccggtc atcctgcaca cgccgctgat gtcaggctcg aacttcgtgc
     2161 acggcatcgt cgtcgtcggc gcgatggtcg cgctcggcca cgcgcagacc ggcctggagc
     2221 agctgctcgg cttcatcggc gtcgtgctcg ccgccggcaa tgcggtcggc ggctacgtgg
     2281 tcaccgagcg catgctcgaa atgttcaagt cgagcaagga gtaagcgaac gtggccggac
     2341 tcatccagat cgtttacttc gcggccgcgg cgctgttcat cctcggcctc aagcgcatgt
     2401 cctcgccgaa gacggcgcgc agcggcatcg tctgggccgg tgccggcatg gcgctcgcca
     2461 cgctggtcac cttcctgtac ccgggcctgt cgaactacct gctgatcctg gtcgcgatcg
     2521 gcgtcggttc ctatgcggcc tgggtcagcg gcaagaaggt cgcgatgacc gacatgccgc
     2581 agatgatcgc gatctacaac ggcatgggcg gcggtgccgc ggcctgcatc gccgcggtcg
     2641 agctgctcaa gggtggcagc cacggtgccg tgcatgcgct gctcggcgtg ctcggtgcgc
     2701 tgatcggcgc cgtgtcgttc tcgggctcgg tggtcgcgtg ggccaagctc gacggacgca
     2761 tgaagaaggc gatccgcttc ccgaggcaga agctcgtcaa cctcggcgtg ttcgtgctgg
     2821 cgctgctgtt cgggctgatc gtcatgggcg cgagccagcc cggtgcgttc agcatcatcg
     2881 ccttcttcct gctggcgctc gccttcggcg cgatgatggc gctgccgatc ggcggtgccg
     2941 acatgccggt ggtcatctcg ctgttcaacg ccttcaccgg tcttgcggtc gcgtttgaag
     3001 gctacgtgat gcagaacgcg gcgatgatca tcgccggaac cgtcgtcggt gcggccggtt
     3061 cgctgctgac gcagctgatg gcgaaggcca tgaaccgccc gatcagcaac gtgctgttct
     3121 cgaacttcgg cggcggcagc ggtgcgacgg ccgaggaaga ggtcaagggc tcgatgaagg
     3181 ccagcgagcc gcaggatgcg gcggtgatga tggcctacgc ggccaaggtc atcatcgtgc
     3241 ccggctacgg catggcggtc gcgcaggccc agcacaaggt ctgggagctc gccgagctgc
     3301 tcgaagagaa cagcgtcgag gtcaagttcg cgatccatcc ggtcgccggc cgcatgcccg
     3361 gccacatgaa cgtgctgctc gccgaggccg gcgtgccgta cgacaagatc ttcgacctcg
     3421 acgagatcaa caacgagttc gcgtcgaccg acgtggtgct ggtcatcggc gccaatgacg
     3481 tcgtcaaccc ggtcgccaag tccgatccga acagcccgat ctacggcatg ccggtgctga
     3541 acgccgacca ggccaggaac gtgctggtga tcaagcgcgg tcagggtgca ggcttctcgg
     3601 gcatcgagaa ccacctgttc tacctcgaca acacgcgcat ggtgtacggc gacgcgcaga
     3661 aggtgctcgc cgagctgatc ggcttcgtca agacgctctg atcgcggcgc ggcccgcagc
     3721 cggattcgtc gtgcgcgacg gccggccgcg gatgccctcc gcacgaggcg gcgctccctg
     3781 cggggcgccg cctttttcct gtgcgcgtca gtccgccggc ccgggcggcg gtacgcgcag
     3841 ccagaaggtc accggcccgt cat
//
LOCUS       DAOQJH010000193         3860 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_14598_length_3860_cov_4.772142, whole genome shotgun sequence.
ACCESSION   DAOQJH010000193 DAOQJH010000000
VERSION     DAOQJH010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3860)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3860)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3860
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14598_length_3860_cov_4.772142"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1560)
                     /locus_tag="PLQ18_05605"
     CDS             complement(<1..1560)
                     /locus_tag="PLQ18_05605"
                     /inference="COORDINATES: protein motif:HMM:NF023273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNE01162.1"
                     /translation="MVPSFPLRLRTRFRLLLAASVLGIALVDALVAWSNLDERVYTEL
                     LHEARDVRDVLLGTREVYRALLPDGDSAPLDAHMLALLPSHALLRIARDFPAPDDAEA
                     SLRLAVVSDRPLDPANRADAGQQQALDWLRAHPQAGEHMREVRDSAGERHLLYALPIR
                     FDGWCLRCHAPTASAGETGIHGLLSLRSPLAPAREREFAEWSHNFAGRMLAYIALFGL
                     LGLLVDRGFVRRLAQLERAALAVADGDASVRVQSDGPRPAQDELATLGQAFDAMAAAV
                     QARTGELQTALAQYRYLFAHSPLPMLVFDRTTLAILDVNEAALAHYGYAREAFLQLTI
                     RELRPPEDVALMQQLRAAIAAAGEIPACHTVRHRRSDGTLLDVNVWTRALDFDGRPAR
                     IALIQDVSEQQRALAALRLSQLKYQTIFRTSPDLIAITERASGRFLEVNDAYTRIMGY
                     TAEEAIGRTSAELGTWGAPDSRAQMIAALGEGVRLMNFQTLFRRKDGEVFPALLSLEA
                     ARIGEHDCFIIS"
     gene            complement(1631..2407)
                     /locus_tag="PLQ18_05610"
     CDS             complement(1631..2407)
                     /locus_tag="PLQ18_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018860993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HNE01163.1"
                     /translation="MRIITLNCNGIRSAAGKGFFEWLAGQQADIVCLQETKSQEHQRG
                     REFRPQGWHCHYVDAVRPGYSGVAILSKREPDAVVTALGAGFEDMDAEGRYLEARFGA
                     LSVVSLYLPSGSSGELRQQVKYSFMDRFAGHLAGLRARGGEALVCGDWNIAHRPADLR
                     NWKSNQKNSGFLPEERAWLDRLYGEAGWVDVWRRLYPEDGEACYTWWSNRGQAWAKNV
                     GWRLDYHVACPGFAGRAQAGSVYKAARFSDHAPVTIDYAD"
     gene            complement(2881..>3860)
                     /locus_tag="PLQ18_05615"
     CDS             complement(2881..>3860)
                     /locus_tag="PLQ18_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018233159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HNE01164.1"
                     /translation="KDEYNPDKLRYHRIIIMTDADVDGSHIRTLLLTFFYRQMKELVE
                     RGHVYIAQPPLYKVKKGKQEQYVKDDGELSAYLLSAALDGAELLPDAVSPPISGAALE
                     TLARDSMAVAQVIRRLARRFDARVLEQLVQLPRLRAETLREGRGLEGWIKTLGERLAA
                     AADDGSQYTVRLDAREDGEGHCLTVVQRRHGLERESRFAAEFFATPEYGLLAQLADRI
                     ADLFSAETTIRRGERSQGVDSLRATVDWLLDEARRGQHIQRYKGLGEMNPGQLWETTM
                     NPETRRLLQVRIEDAIAADEVFTTLMGDEVEPRREFIESNALAAANIDV"
BASE COUNT          601 a         1321 c         1332 g          606 t
ORIGIN      
        1 cgagatgatg aagcagtcgt gctcgccgat gcgcgcggcc tccagcgaca gcagcgccgg
       61 gaacacctcg ccgtccttgc gccggaacag ggtctggaag ttcatcaggc gcacgccctc
      121 gccgagcgcg gcgatcatct gcgcacggct gtccggcgcg ccccaggtgc cgagttcggc
      181 cgaggtgcgg ccgatggcct cctcggccgt gtaccccatg atgcgcgtat aggcatcgtt
      241 gacttcgagg aagcgcccgc tggcgcgctc ggtgatcgcg atcaggtccg gcgaggtgcg
      301 gaagatggtc tggtacttca gctgcgagag cctgagcgcc gccagcgcac gctgctgctc
      361 gctgacatcc tggatcagcg cgatgcgcgc cggccggccg tcgaaatcca gcgcccgcgt
      421 ccagacgttg acatcgagga gggtgccgtc gctgcggcgg tggcgcacgg tgtgacaggc
      481 cggaatctcg ccggcagcgg cgatcgcggc gcgcagctgc tgcatcagcg ccacgtcctc
      541 gggcgggcgc agctcacgga tcgtcagctg caggaacgcc tcgcgcgcat agccgtagtg
      601 ggcgagggcg gcctcgttga catcgaggat cgcgagcgtc gtgcggtcga agaccagcat
      661 cggcagcgga ctgtgcgcga acaggtagcg gtactgcgcg agcgccgtct gcagttcgcc
      721 ggtgcgcgcc tgcaccgccg ccgccatcgc atcgaaggcc tgaccgagcg tggccagctc
      781 gtcctgcgcc ggccggggcc cgtcgctctg cacgcgcacc gaggcatcgc catcggccac
      841 cgccagtgcc gcccgttcca gctgcgccag ccggcgcacg aagccgcgat cgaccagcag
      901 gccgagcagc ccgaacagag cgatgtaggc gagcatgcgg cccgcgaagt tgtggctcca
      961 ttccgcgaat tcccgctcgc gggccggggc cagcggcgag cgcaggctca gcaggccgtg
     1021 gatgccggtc tcgccggccg atgccgtggg agcatggcag cgcaggcacc agccatcgaa
     1081 gcggatcggc agcgcgtaca gcagatgccg ctcgccggcg ctgtcgcgta cctcgcgcat
     1141 gtgctcgccg gcctgcggat gagcccgcag ccagtccagc gcctgctgct gcccggcatc
     1201 ggcgcggttg gccggatcga ggggccggtc cgagacgacc gccagccgca gcgaagcctc
     1261 cgcatcgtcg ggggccggga agtcgcgggc gatgcgcagc agcgcatgcg agggcagcag
     1321 cgccagcatg tgcgcatcga gcggcgcgga atctccgtcg ggcaggagcg cgcggtagac
     1381 ctcccgcgtg ccgagcagca cgtcacgcac gtcgcgcgcc tcgtgcagca gctccgtgta
     1441 gacgcgttca tcgaggttgg accaggcaac cagcgcatcg accagcgcga tgccgagcac
     1501 cgaggccgcc agcagcagcc ggaaccgggt acgcagccgc agggggaaac tcggcaccat
     1561 cgtggaatcg ggatcggcag cggctcaggg atcggaaaac ctgatcatca tgatggtttt
     1621 gtaagccgaa tcaatccgcg taatcgatcg ttaccggcgc atggtcggaa aagcgcgcgg
     1681 ccttgtagac gctgccggcc tgcgcccggc cggcgaagcc cgggcaggcg acgtggtagt
     1741 cgagacgcca gccgacgttc ttcgcccagg cctggccgcg gttcgaccac cacgtgtagc
     1801 aggcctcgcc gtcctccggg tacagccggc gccagacgtc gacccagccg gcctcgccgt
     1861 acagccggtc cagccaggca cgctcttcgg gcagaaagcc gctgttcttc tggttggatt
     1921 tccagtttct gagatccgcc ggccggtgcg cgatgttcca gtcgccgcag accagcgcct
     1981 cgccgccgcg cgcgcgcaag cccgccagat gcccggcgaa gcggtccatg aacgaatact
     2041 tgacctgctg gcgcagctcg ccgctcgatc ccgagggcag gtacagcgac acgacgctca
     2101 gggccccgaa gcgggcttcg agatagcggc cttcggcatc catgtcctcg aagccggccc
     2161 cgagcgcggt gacgacggca tcgggctcgc gcttcgacag gatcgccact ccgctgtagc
     2221 cggggcgcac ggcatcgacg taatggcagt gccagccctg cggccggaat tcacggccac
     2281 gctggtgttc ctgagacttg gtttcctgca ggcagacgat gtcggcctgc tgcccggcga
     2341 gccattcgaa aaagcccttg ccggccgcgg aacggatgcc gttgcagttc agcgtgatga
     2401 tgcgcatcgg gaagattcac ctgtcgccgg attccgggca tccggcgcat ttgcgaagac
     2461 cgggcgatgc cggcaaatgg atttgtctgc tggacggatg cgtttccgtt tcaccatccc
     2521 catcacgggc cggtgcatcg gcagccgcga gggcgggaat ggcggttgat gatttctgga
     2581 aaaccggccg gaccgggatc tacggtcccg aattgtccac agcggacatt cgctgcatga
     2641 cgctccggct ctgcaagcct gccggggatt gcggatcaga ccaggcgtct tttatcgagt
     2701 cacggcaaag ccggtcatgc cggaattgcc gtggcagcat tcagtaaatg ccgtcacacc
     2761 ggacttgtcc gcaatcagca caccgcatcc acagcgccat ccaccgtttc tgtggattac
     2821 ggagttgctg ccgccggggc tgtcgcgccg cggtcacgac ggaccgcggc gcacgggcgt
     2881 tcagacgtcg atgttcgccg ccgccagcgc gttgctttcg atgaactcgc gccgcggctc
     2941 gacctcgtcg cccatcagcg tcgtgaacac ctcgtcggca gcgatcgcat cctcgatgcg
     3001 cacctgcagc aggcggcggg tttccgggtt catcgtcgtt tcccagagct gcccggggtt
     3061 catctcgccg aggcccttgt agcgctggat gtgctggccg cggcgtgcct cgtcgagcag
     3121 ccagtcgacg gtggcacgca ggctgtcgac gccctggctg cgctcgccgc ggcggatcgt
     3181 ggtctcggcg ctgaacagat cggcgatgcg gtcggcgagc tgcgcgagca ggccgtattc
     3241 cggcgtcgcg aagaactcgg ccgcgaagcg cgattcgcgc tccagcccgt ggcggcgctg
     3301 cacgaccgtc aggcagtgac cctcgccgtc ctcgcgcgca tcgaggcgca ccgtgtactg
     3361 cgagccgtcg tcggccgccg ccgccagccg ctcgccgagc gtcttgatcc agccctcgag
     3421 gccgcggccc tcgcgcaggg tttcggcgcg cagccgcggc agctgcacga gctgttcgag
     3481 cacgcgcgca tcgaagcgcc gtgcgagccg gcggatcacc tgcgcgaccg ccatcgaatc
     3541 gcgtgccagg gtttccagcg ccgcgccgct gatcggcggg ctgacggcat cgggcagcag
     3601 ctcggccccg tcgagcgcgg ccgacagcag ataggccgac agctcgccgt cgtccttcac
     3661 gtactgttcc tgcttgccct tcttgacctt gtacagcggc ggctgcgcga tgtagacgtg
     3721 gccgcgctcg accagctcct tcatctgccg gtagaagaag gtcagcagca gcgtgcggat
     3781 gtgcgagccg tcgacgtcgg cgtcggtcat gatgatgatg cggtggtaac gcagcttgtc
     3841 cgggttgtac tcgtccttgc
//
LOCUS       DAOQJH010000194         3859 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_14603_length_3859_cov_3.624869, whole genome shotgun sequence.
ACCESSION   DAOQJH010000194 DAOQJH010000000
VERSION     DAOQJH010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3859)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3859)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3859
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14603_length_3859_cov_3.624869"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..296
                     /locus_tag="PLQ18_05620"
     CDS             <1..296
                     /locus_tag="PLQ18_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009323095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HNE01165.1"
                     /translation="GGAGRWRGGDGGVRRLRFRAPMTAAILSDHRRVPPQGMAGGGPG
                     ACGINRIERADGRIERLGGIATVSMQPGDAFVIETPGGGGYGAPDPSGPTSCG"
     gene            331..1698
                     /locus_tag="PLQ18_05625"
     CDS             331..1698
                     /locus_tag="PLQ18_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005965976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNE01166.1"
                     /translation="MSDELIARARRYATEAHARIDQRRKYTGQPYEVHLKAVAALILR
                     HGGDAAMVAAAWLHDTVEDTPATLGDLEREFGADVAALVAQLTDVSRPADGNRAVRRA
                     IDRAHSAQASARAQTIKLADLIDNCRDLVRHDPAFARTFTAEMALLLDVLVEADPKLR
                     QQARRTLERAQAELERLEHAPPPAPSPPPAPAETERLRRQSRIYAHAFSAQDIAEALP
                     SFDGERPAAAVAASLDAGGHGVAGVRQEGRIVASVKRAALDALGEAACAQAARALTRA
                     ECVASEAGLAEVVHVLTRHDHAFVTLLGEPVAVIGRNDMQKPVVRMWLFGILTLAELD
                     FTGRIRARWPGGEWTAKLSAARLDKARQLQLERQRRGHACTLLDCLQFGDKLRLLIED
                     PAELARLDMPGRSAAKRVIADLESLRNHLAHAQDIVSHDWAQIARLARRIEEMSSGTG
                     LPEGG"
     gene            1753..2106
                     /locus_tag="PLQ18_05630"
     CDS             1753..2106
                     /locus_tag="PLQ18_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003198223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HNE01167.1"
                     /translation="MQPLLGVRFFRSAAGTEPVREWLRDLPVEHRRAIGTDIKTVQYG
                     WPLGMPLVRKLEPGLWELRSHIADGIARVLFTVHGADLVLLHGFIKKSGRIPVDELDT
                     ARQRLALFSRWADRD"
     gene            2045..2383
                     /locus_tag="PLQ18_05635"
     CDS             2045..2383
                     /locus_tag="PLQ18_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007244504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNE01168.1"
                     /translation="MNSTPLASVWRCFQGGQTVTDHPHIGSDFDDFLAEEGLLESAGA
                     LAIKRVVAWQIREAMKAQKLSKKAMAERMGTSRAALDRVLDENDPGMTLATLARAARA
                     LGKRVEIKVS"
     gene            complement(2444..3175)
                     /locus_tag="PLQ18_05640"
     CDS             complement(2444..3175)
                     /locus_tag="PLQ18_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015467131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HNE01169.1"
                     /translation="MEILLDPQTWLSLLTLTALEIVLGIDNLVFISILSSRLPAHQQS
                     TARRVGLALAVITRLLLLLSISWLAGVTAPLFSVFDHPVSLRDLILLGGGLFLLAKGT
                     TEIHATVEGVEEEIRESKAVSFTSVVVQIMILDIVFSLDSVITAVGMASEISVMAAAV
                     IIAVLVMVFAADPLSRFIEEHVSVKMLALSFLILVGVALIADGMGFHIPKGYLYFAIA
                     FSVGVETLNILAKKKRMKAKAEAAH"
     gene            3431..>3859
                     /locus_tag="PLQ18_05645"
     CDS             3431..>3859
                     /locus_tag="PLQ18_05645"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HNE01170.1"
                     /translation="MSVLVTREAPDFTAAAVLADGSIVEDFSLSSLRGKYVVLFFYPL
                     DFTFVCPSEIIAHGNRVAKFKELGVEVVGVSIDSQFTHFAWRETPVEKGGIGRVPFTL
                     VADVKHDICRAYGVEHPGAGVALRGSFLIDKDGVVQAQQVN"
BASE COUNT          630 a         1330 c         1336 g          563 t
ORIGIN      
        1 cgggcggcgc cgggcgctgg cgcggcggcg acggcggcgt gcgccggctg cgcttccggg
       61 cgccgatgac ggcggcgatc ctgtcggatc atcggcgcgt gccgccacaa ggcatggccg
      121 gcggtggtcc cggcgcctgc ggcatcaacc gcatcgagcg cgccgacggc cgtatcgagc
      181 ggctcggcgg catcgccacg gtgtcgatgc agcccggcga tgccttcgtg atcgagacac
      241 cgggcggcgg cggctacggc gcgccggacc cgtccgggcc cacgtcctgc ggatgagcag
      301 ggttcgtccg tttccggagc cgccagcgcc atgtccgacg aactgatcgc ccgcgcccgc
      361 cgctacgcca ccgaggcgca cgcgcgcatc gaccagcggc gcaagtacac cggccagccc
      421 tacgaggtgc acctgaaggc ggtcgcggcg ctgatcctgc gccacggcgg cgatgcggcg
      481 atggtcgccg ccgcctggct gcacgatacg gtcgaggaca cgccggcgac gctcggtgac
      541 ctcgaacgcg agttcggcgc cgatgtcgcc gcgctggtcg cgcagctgac cgacgtgagc
      601 cgcccggccg acggcaaccg cgccgtgcgc cgcgccatcg accgggcgca cagcgcgcag
      661 gcttcggcgc gcgcgcagac catcaagctc gccgacctga tcgacaactg ccgcgatctg
      721 gtgcgccacg acccggcctt cgcccgcacc ttcacggccg agatggcgct gctgctcgac
      781 gtgctggtcg aggccgaccc gaagctgcgc cagcaggccc ggcgcacgct ggagcgggcg
      841 caggccgagc tcgaacggct tgagcacgcg ccgccgccgg ctccaagccc gccgcccgca
      901 ccggccgaga ccgaacgcct gcgccggcag agccgcatct acgcgcatgc gttctcggcg
      961 caggacatcg ccgaggcgct gccgtccttc gacggcgaac gcccggccgc cgcggtcgct
     1021 gccagcctcg atgccggcgg ccacggtgtg gccggggtgc gtcaggaggg ccgtatcgtc
     1081 gccagcgtga agcgcgccgc gctcgatgcg ctcggcgagg ccgcctgcgc gcaggccgcg
     1141 cgggcgctga cgcgcgccga atgcgtggcc agcgaggccg gactcgccga ggtcgtgcac
     1201 gtgctgaccc gccacgacca cgccttcgtg acgctgctcg gcgagccggt cgcggtcatc
     1261 gggcgcaacg acatgcagaa gccggtcgtg cgcatgtggc tgttcggcat cctgacgctc
     1321 gccgaactcg acttcaccgg ccgcatccgc gcccgctggc cgggcggcga gtggacggcg
     1381 aagctgtccg ccgcgcgcct cgacaaggcg aggcagctgc agctcgaacg ccagcgccgc
     1441 ggccacgcct gcacgctgct cgactgcctg cagttcggcg acaagctgcg cctgctgatc
     1501 gaggacccgg ccgaactcgc ccgcctcgac atgcccggac gcagcgccgc gaagcgcgtg
     1561 atcgccgacc tcgaatcgct gcgcaaccac ctcgcacacg cgcaggacat cgtcagtcac
     1621 gactgggcgc agatcgcgcg gctggcgcgg cggatcgagg agatgagcag cgggacgggg
     1681 ctgccggagg gagggtgacg aggctgaggg cggcatgtct gcctgtgcat caaaaaatgt
     1741 acactcccgg ccatgcagcc cttgctcggt gtccggttct ttcgcagcgc ggccggtacc
     1801 gagccggtcc gggaatggtt gcgggatttg cccgtcgagc atcgcagggc catcggcacc
     1861 gacatcaaga ccgtgcagta cggctggccg ctcggcatgc cgctggtgcg caagctcgaa
     1921 cccggactct gggaactgcg ttcgcacatc gccgacggta ttgcgagagt gctgttcacc
     1981 gtgcatgggg ccgatctggt gctgctgcat ggcttcatca agaaatccgg caggattccg
     2041 gtcgatgaac tcgacaccgc tcgccagcgt ctggcgctgt tttcaaggtg ggcagaccgt
     2101 gactgatcat ccgcacatcg gcagcgattt cgatgatttc ctggccgagg aaggcctgct
     2161 ggaaagcgcg ggagcgctgg ccatcaagcg cgtggtcgcc tggcagatac gggaagccat
     2221 gaaggcccag aagctcagca agaaggcaat ggccgagcgc atggggacga gccgggcggc
     2281 gctcgaccgg gtgctggacg aaaacgatcc cggcatgacc ctcgccacgc ttgcccgtgc
     2341 tgcccgggcg ctcggcaagc gggtcgagat caaggtttcc tgaagcggtc gcttcaggcc
     2401 ggatgcctgc ccgccagagc aggcatccgg tttctgaacc ccgtcagtgc gccgcttcgg
     2461 ccttcgcctt catccgcttc ttcttcgcga ggatgttcag cgtctcgacg ccgaccgaga
     2521 acgcgatcgc gaagtacagg tagcccttcg ggatgtggaa gcccatgccg tcggcgatca
     2581 gcgcgacgcc gaccaggatc aggaaggaca gcgccagcat cttcaccgac acgtgctcct
     2641 cgatgaagcg cgacagcggg tcggccgcga acaccatgac cagcaccgcg atgatgaccg
     2701 ccgcggccat caccgagatt tcgctggcca tgccgaccgc cgtgatcacc gagtcgagcg
     2761 agaacacgat gtcgaggatc atgatctgca cgaccaccga cgtgaagctg accgccttcg
     2821 actcgcggat ttcctcctcg acgccctcga cggtggcgtg gatttcggtc gtgcccttgg
     2881 cgagcaggaa caggccgccg ccgagcagga tcaggtcgcg caggctcacc gggtgatcga
     2941 acaccgagaa cagcggcgcg gtgacgccgg ccagccacga gatcgacagc agcagcagca
     3001 ggcgcgtgat cacggccagc gccagaccga cgcgccgggc cgtgctctgc tgatgcgcgg
     3061 gcaggcgcga ggacaggatc gagatgaaca ccaggttgtc gatgccgagc acgatctcga
     3121 gcgcggtcag cgtcagcagg ctcagccagg tttgcgggtc cagcaggatt tccatggatg
     3181 ggctactcgt cggaggaggc gggcgaaggc gcggggaagg cgcgggaaaa gggcatgagc
     3241 ggggctgcga agcgcgagta tagaatccgg cggctgcctt acaggtgatg attcgttgag
     3301 cgcgctgata ggcgcgcgct atcgtcgtga tcggaaattt tcaattcccc taccggttcg
     3361 cggcaaggct atgctgcgcc gcgtctacga cttccgtagt ccttacaagg ccagccaagg
     3421 agatccattc atgagcgtac tcgtcacccg cgaagccccg gacttcaccg ccgctgccgt
     3481 gctcgccgac ggcagcatcg tcgaggactt ctcgctctcc agcctgcgcg gcaagtacgt
     3541 ggtgctgttc ttctacccgc tcgacttcac cttcgtctgc ccgtccgaga tcatcgccca
     3601 cggcaaccgc gtcgcgaagt tcaaggaact cggcgtcgag gtggtcggcg tgtcgatcga
     3661 ctcgcagttc acgcacttcg cgtggcgcga gacgccggtc gagaagggcg gcatcggccg
     3721 cgtgccgttc acgctggtcg ccgacgtcaa gcacgacatc tgccgcgcct acggcgtcga
     3781 gcacccgggc gccggcgtcg cgctgcgcgg ctccttcctg atcgacaagg atggcgtcgt
     3841 gcaggcccag caggtcaac
//
LOCUS       DAOQJH010000195         3850 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_14657_length_3850_cov_5.410540, whole genome shotgun sequence.
ACCESSION   DAOQJH010000195 DAOQJH010000000
VERSION     DAOQJH010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3850)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3850)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3850
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14657_length_3850_cov_5.410540"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1274)
                     /locus_tag="PLQ18_05650"
     CDS             complement(<1..1274)
                     /locus_tag="PLQ18_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013640057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HNE01171.1"
                     /translation="MSTLHSSLNPRAGEFRRNGEAMAALVADLRAQVADIARGGGDAA
                     MRRHVARGKLPVRERIRTLLDVGSPFLECSQLAAHGVYAEPVPAAGLVTGIGRIHGRE
                     CVIVANDATVKGGTYYPLTVKKHLRAQEIARENHLPCVYLVDSGGAHLPNQDEVFPDR
                     EHFGRIFYNQATLSALGIAQIAVVMGSCTAGGAYVPAMADESIIVRAQGTIFLAGPPL
                     VRAATGEVVSAEELGGADVHCRRSGVTDHYALDDAHALGLARRAVANLNRVKPVGLAL
                     LPARAPLHPPEELHGVIPADPRQPYDVREVIARLVDASEFDEFKALYGPTLVTGFAHL
                     YGYPVGIVASNGVLFSEAALKGAHFVELCCQRGIPLVFLQNITGFMVGRRYEAGGIAK
                     DGAKMVTAVACAQVPKFTVLIGGSFGAGNYGM"
     gene            1477..1860
                     /locus_tag="PLQ18_05655"
     CDS             1477..1860
                     /locus_tag="PLQ18_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HNE01172.1"
                     /translation="MIQAFAVLLFFQFLGEVGVQGFGLPFPGPLLGMLLLFVALLVHG
                     RVPEGLRALSQSLIGNLMLLFIPAVSGVMIHWQKVAADGVAFVVSGVLATALTLVVTA
                     LLLKHLLARRAHGDADNGPDAGTPS"
     gene            1857..2573
                     /locus_tag="PLQ18_05660"
     CDS             1857..2573
                     /locus_tag="PLQ18_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HNE01173.1"
                     /translation="MNDLFQLWVYLAASPLLWLLVTVLAYLTGVWLFRISKANPLFTP
                     VLVGVVIVVSVLLVTGTPYPVYFEGAKFVHFLIGPATVALAVPLYSVFGRLKTMLRPL
                     LIALFAGALASIGSAIGIAWALGASRATLMSLAPKSVTTPIAMGVAERIGGIPSLAAV
                     AVAVTGISGAMMARGLLNRLRIEDMAVRGFAAGLAAHAVGTARAHQIHPDAGAFAALA
                     MGLNGIVTALLLPLLVRLSG"
     gene            2675..3163
                     /gene="bfr"
                     /locus_tag="PLQ18_05665"
     CDS             2675..3163
                     /gene="bfr"
                     /locus_tag="PLQ18_05665"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012974948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HNE01174.1"
                     /translation="MQGNPQIIATLNQLLTGELSAADQYLYHAHRYLDWGLHALWERT
                     HHEMEEERHHAQVLIERILFLEGRPDVASRDPLALGSDVESMLKNDLALEYQVVGALR
                     AAIAQCEQLGDYQTRNELLPLLADTEEDHAHWLEQQLGLIDRIGLENYLQSQMGQAPS
                     AS"
     gene            complement(3263..3724)
                     /locus_tag="PLQ18_05670"
     CDS             complement(3263..3724)
                     /locus_tag="PLQ18_05670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HNE01175.1"
                     /translation="MNMPVRGRGIALGIIALGFATTAAAGSAVGSTMVLAAAPTEMPV
                     IQQSGTIRYLSGGVGDEERTAMNQVENQFNLGITLATANGSFLSDAQIRISDSKGSTV
                     LETVPNGPMLFVQLPPGEYKVDAQGFGQHYSKTVHVGERMSKITFTWPAQG"
BASE COUNT          569 a         1316 c         1337 g          628 t
ORIGIN      
        1 cacatgccgt agttgccggc cccgaagctg ccgccgatca gcaccgtgaa cttcggcacc
       61 tgcgcgcagg cgacggcggt gaccatcttc gcgccgtcct tggcgatgcc gccggcctcg
      121 tagcggcgcc cgaccatgaa gccggtgatg ttctgcagga acaccagcgg gatgccgcgc
      181 tggcagcaga gctcgacgaa gtgcgcgccc ttcagcgccg cctccgagaa cagcacgccg
      241 ttgctggcga cgatgccgac cggatagccg tacaggtgcg cgaagccggt gaccagcgtc
      301 ggaccgtaca gcgccttgaa ctcgtcgaac tcgctggcat cgacgaggcg cgcgatgacc
      361 tcgcgcacgt cgtagggctg gcgcggatcg gccgggatca cgccgtgcag ctcttcgggc
      421 ggatgcagcg gcgcgcgcgc cggcagcagc gcgagtccga cgggcttgac gcggttcagg
      481 ttcgcgaccg cgcgccgcgc cagaccgagc gcgtgcgcat cgtcgagcgc gtagtgatcg
      541 gtgacgcccg agcggcggca gtgcacgtcg gcaccgccga gttcctcggc actgaccacc
      601 tcgccggtcg cggcgcgcac gagcggcggg ccggccagga agatcgtgcc ctgagcacgc
      661 acgatgatcg attcgtcggc catcgccggc acataggcac cgccggccgt gcacgagccc
      721 atgaccacgg caatctgcgc gatgccgagc gccgagagcg tcgcctggtt gtagaagatg
      781 cggccgaagt gctcgcgatc cggaaagacc tcgtcctggt tcggcaggtg cgcaccgccg
      841 gaatcgacca ggtacacgca gggcagatgg ttctcgcgcg cgatctcctg cgcgcgcagg
      901 tgcttcttca ccgtcagcgg gtagtaggtg ccgcccttga ccgtggcgtc gttggcgacg
      961 atcacgcatt cgcggccgtg gatgcggccg atgccggtga cgagcccggc ggccggcacc
     1021 ggctcggcgt acaccccgtg cgcggcgagc tgcgagcact ccaggaacgg cgagccgacg
     1081 tcgagcagcg tgcggatgcg ctcgcgcacc ggcagcttgc cgcgcgcgac gtgccggcgc
     1141 atcgccgcat cgccgccgcc gcgcgcgatg tccgcgacct gcgcgcgcag gtcggcgacc
     1201 agtgcggcca tggcttcgcc attgcggcgg aactccccgg cgcgcgggtt caggctcgaa
     1261 tgcagcgtgc tcatcggcag gctccgggcg ggacggcggc gcgaatctag ttgacgcacg
     1321 cgtcagccgc ctgccgctgg tgcatcggcg tctggctggc atgtgccgca tgcgtccgcc
     1381 ggccggagct ggctataagc gcaacaccac gctgatcccc ccggcgttcc ttcccccctg
     1441 cccggccggc gtgtcacggc cgaggagttg ttcgcgatga tccaggcgtt tgccgtgctg
     1501 ctgttcttcc agttcctcgg cgaggtcggt gtgcagggct tcggcctgcc gtttccgggg
     1561 ccgctgctcg gcatgctgct gctgttcgtg gcgctgctcg tgcacgggcg cgtgcccgaa
     1621 gggctgcggg cactgtcgca gtcgctgatc ggcaacctga tgctgctgtt catcccggcg
     1681 gtcagcggcg tgatgatcca ctggcagaag gtcgccgccg atggcgtggc cttcgtcgtc
     1741 tccggcgtgc tggcgacggc gctgacgctg gtcgtgaccg cgctgctgct gaagcatctg
     1801 ctggcacgac gggcgcacgg cgacgcggat aacggtccgg atgcgggtac gccgtcatga
     1861 acgacctgtt ccagctctgg gtgtatctgg ccgcctcgcc gctgctgtgg ctgctcgtga
     1921 ccgtgctcgc gtatctgacc ggcgtctggc tgtttcgcat cagcaaggcc aatccgctgt
     1981 tcacgccggt gctggtcggc gtcgtcatcg tggtttcggt gctgctcgtg accggcacgc
     2041 cgtatccggt gtacttcgag ggcgcgaagt tcgtgcactt cctgatcggc ccggcgaccg
     2101 tggcgctggc cgtaccgctg tacagcgtgt tcggccggct gaagacgatg ctccggccgc
     2161 tgctgatcgc gctgttcgcc ggcgcgctgg cctcgatcgg cagtgccatc ggcatcgcct
     2221 gggcgctcgg tgcctcgcgc gcgacgctga tgtcgctggc gccgaagtcg gtgacgacgc
     2281 cgatcgccat gggcgtggcc gagcgcatcg gcggcatccc gtcgctggcg gcggtggcgg
     2341 tggcggtgac cggcatctcc ggcgcgatga tggcgcgcgg cctgctgaac cgcctgcgca
     2401 tcgaggacat ggccgtgcgc ggcttcgccg ccggactggc ggcacacgcg gtcggcaccg
     2461 cccgcgccca ccagatccac ccggacgccg gtgccttcgc ggcgctggcg atggggctga
     2521 acggcatcgt caccgcgctg ctgctgccgc tgctggtgcg gctgtccggc tgaacgaccg
     2581 cggccggaca aatgagaacg gatgacaaat acggccgatc gcgttattga ggcgcggtcc
     2641 gtcccttccc tgttcctgtc aaaggcccgc aacgatgcaa ggcaacccgc agatcatcgc
     2701 caccctgaac cagctgctga ccggcgaact gagcgcggcc gaccagtacc tgtaccacgc
     2761 gcaccgctac ctcgactggg gcctgcacgc gctctgggag cgcacgcacc acgagatgga
     2821 agaggaacgc caccacgcgc aggtgctgat cgaacgcatc ctgttcctcg aaggcaggcc
     2881 cgatgtcgcc agccgcgatc cgctcgcgct cggcagcgat gtcgagtcga tgctgaagaa
     2941 cgaccttgcg ctcgaatacc aggtggtcgg cgccctgcgc gccgcgatcg cgcagtgcga
     3001 gcagctcggc gactaccaga cgcgcaacga actgctgccg ctgctcgccg acaccgagga
     3061 agaccacgcg cactggctgg agcagcagct cggcctgatc gaccggatcg ggctggagaa
     3121 ctacctgcag tcgcagatgg gccaggcacc atcggcgagc tgatcccgcg gtttttccat
     3181 cgcccctgaa aagccagcgc cccgaccgca gcagacggtc ggggcgctgg cttttcaggc
     3241 tttcagccga tgcgggcgat gatcagccct gcgccggcca cgtgaacgtg atcttgctca
     3301 tgcgttcgcc gacgtggacg gtcttcgagt agtgctggcc gaagccctgg gcatcgacct
     3361 tgtattcgcc gggcggcagc tgcacgaaca gcatcgggcc gttcggcacg gtttcgagca
     3421 cggtcgaacc cttgctgtcg ctgatgcgga tctgcgcatc gctcaggaac gaaccgttgg
     3481 ccgtcgccag cgtgatgccg agattgaact ggttctcgac ctgattcatc gcggtgcgtt
     3541 cttcatcgcc gacaccgccg ctcaggtagc ggatcgtgcc ggactgctgg atcaccggca
     3601 tttcggtcgg cgctgccgcc agcaccatgg tcgagccgac cgccgaaccg gccgcagccg
     3661 tggtggcgaa accgagggcg atgatgccga gggcgatacc gcggccgcga acaggcatgt
     3721 tcatgatggc ttcctccggg gggatggcaa aggcccatca gggcgagcac ctgcatcgac
     3781 cacccgcggt gcgccgggtt ccggcagccg acgcaagtac ctgaaaaccc ggtacatcgc
     3841 tgatcaggag
//
LOCUS       DAOQJH010000196         3842 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_14698_length_3842_cov_3.808820, whole genome shotgun sequence.
ACCESSION   DAOQJH010000196 DAOQJH010000000
VERSION     DAOQJH010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3842
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14698_length_3842_cov_3.808820"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="PLQ18_05675"
     CDS             <1..197
                     /locus_tag="PLQ18_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP:cob(I)alamin adenosyltransferase"
                     /protein_id="HNE01176.1"
                     /translation="CRRVERHLVTLVRTEAVNPHAPAYLNRLSDLLFVLGRVLARDEG
                     GSEVLWRNDHGRRRTAADQP"
     gene            194..514
                     /locus_tag="PLQ18_05680"
     CDS             194..514
                     /locus_tag="PLQ18_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2322 family protein"
                     /protein_id="HNE01177.1"
                     /translation="MSFAENLASLPPAGHLAALELFEHGEPAGRIENRPGQAGSLRIY
                     HAVAVNGRIDAEAARRALGLYGEHLADAEANPGRHPNIDRLLALIRRNSVLVIRPVPA
                     ADGA"
     gene            complement(548..1477)
                     /gene="ubiA"
                     /locus_tag="PLQ18_05685"
     CDS             complement(548..1477)
                     /gene="ubiA"
                     /locus_tag="PLQ18_05685"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019366668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HNE01178.1"
                     /translation="MKPSPEPAPAASASGKPRAALCWPRLLAKAGDYAQLARMHRPIG
                     SFLLLWPTLWALWLAGHGQPPEGTVLVFCLGVFLMRSAGCVMNDIADRRFDPHVARTR
                     DRPLAAGRVTVREALWLAIGLSLVAFGLVLTQNGLTIGLSFAGLALAAGYPLMKRLHH
                     LPQVVLGMAFGWGIPMAFAAVTGSVPALAWLLYAANIVWSVAYDTFYAMTDREDDLKI
                     GVKSSAILFGARDRQIVALLQGAFLLLLGLAGLMAGCGPVYYLGLFAAAWLMAWEQYL
                     VRRREPAACFHAFLHNNWVGLAVFAGVLVDALP"
     gene            complement(1487..2080)
                     /locus_tag="PLQ18_05690"
     CDS             complement(1487..2080)
                     /locus_tag="PLQ18_05690"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: protein motif:HMM:NF016248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HNE01179.1"
                     /translation="MAQAPASFRDFTLQSRWQPAARLRRAEMGERLRDCLLGRGSLTA
                     RLRAECARHGRAFSVRVLSERAGRVLREEADALGLRRGVTAWVREVHLLAGGVPWVFA
                     RTVIPRRTLRGHGRTLTGLGTRPLGAVLFADPGVVRGPLQIARLDLRCPLARRALDVR
                     GASGLPLWGRRSTFRIDGRPLLVCEVFLPALADVLQA"
     gene            complement(2231..3304)
                     /locus_tag="PLQ18_05695"
     CDS             complement(2231..3304)
                     /locus_tag="PLQ18_05695"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006890358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HNE01180.1"
                     /translation="MPLVAHSGLPSFGRLAEEGEEILAQARAAQQDIRELHIGLLNMM
                     PDAALAATERQFMRLIGSSNRIAQFYVHPFTVGGIGRGAEAEAYIGRWYESFDTIRAQ
                     GLDALIITGANISNPDFTQEPFWGPLSEVVEWAHEQVTTTLFACLAAHATMLMKYGVH
                     RRRLPDKAWGVFPHRVVDRRHPLVRNINTRFDVPHSRWNEITREQLDDAGLVTLVESA
                     EVGPHLVVSPDGFRQVFFQGHPEYDIDSLLKEYKREVLRYLHGERADYPPLVQHYFPE
                     AAQTLLYEYKNHVFQQQDHGRQPDGSDFPEAAVLRLLDNTWADTAKAIFNNWLGLMYQ
                     ITGHERAVPFMPGVDPDDPLNGL"
     gene            complement(3398..>3842)
                     /locus_tag="PLQ18_05700"
     CDS             complement(3398..>3842)
                     /locus_tag="PLQ18_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015479568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HNE01181.1"
                     /translation="LVATTVIEVGVDVPNASVMVIENAERLGLAQLHQLRGRVGRGAA
                     ASSCVLMYHPPLSEQGRARLGVLRETSDGFVIARRDLELRGPGGVLGTRQTGTLALRI
                     ADLLRDEPLLPAVQRAADRLLVEHPNAAAALARRWTGERERYGEV"
BASE COUNT          572 a         1383 c         1404 g          483 t
ORIGIN      
        1 tctgccggcg cgtcgaacgg catctggtga cgctggtgcg caccgaggcc gtcaacccgc
       61 acgcgccggc ctacctgaac cggctgtcgg acctgctgtt cgtgctcggg cgcgtgctcg
      121 cacgcgacga aggcggcagc gaggtgctct ggcgcaacga ccacggccgg cggcgcacgg
      181 cggcggacca gccatgagct tcgccgaaaa cctcgccagc ctgccgccgg ccgggcatct
      241 ggccgcgctc gaactgttcg agcacggcga gccggccggg cgcatcgaga accgccccgg
      301 ccaggccggg tcgctgcgca tctaccacgc ggtcgcggtc aacggccgca tcgacgccga
      361 agcggcgcgc cgggcgctcg ggctctacgg cgaacacctc gccgatgccg aggccaatcc
      421 cggccggcat ccgaacatcg accggctgct ggcgctgatc aggcgcaaca gcgtgctggt
      481 gatcaggccc gtcccggccg cggacggcgc ctgattccgg ccggtcacgg cgcaccggcg
      541 gccccgctca cggcagcgca tccaccagca cgcccgcgaa caccgcgagt cccacccagt
      601 tgttgtgcag gaaggcgtgg aagcatgcgg ccggctcgcg tctgcgcacc agatactgtt
      661 cccaggccat cagccaggcc gcggcgaaca ggccgaggta gtagaccggc ccgcagccgg
      721 ccatgagtcc ggccagcccc agcagcagca ggaacgcgcc ctgcagcagc gcgacgatct
      781 gccggtcgcg ggcgccgaac aggatggcgc tcgacttgac gccgatcttc aggtcgtcct
      841 cgcggtcggt catcgcgtag aaggtgtcgt aggccaccga ccagacgatg ttggccgcat
      901 acagcagcca cgcgagcgcc ggcaccgagc cggtcacggc cgcgaaggcc atcgggatgc
      961 cccagccgaa ggccatgccg agcacgacct gcggcaggtg atgcaggcgc ttcatcagcg
     1021 gatagccggc ggcgagcgcg agcccggcga aggacaggcc gatggtcagg ccgttctgcg
     1081 tcagcaccag cccgaaggcg acgaggctca ggccgatcgc gagccacagc gcctcacgca
     1141 ccgtcacgcg gccggcggcg agcggccggt cgcgggtgcg cgcgacgtgc gggtcgaagc
     1201 ggcggtcggc gatgtcgttc atcacgcagc cggccgagcg catcaggaac acgccgaggc
     1261 agaacaccag caccgtgccc tcgggcggct ggccgtgacc ggcgagccac agcgcccaca
     1321 gcgtcggcca cagcagcagg aagctgccga tcggccggtg catgcgcgca agctgcgcgt
     1381 agtcaccggc cttggcgagc agccgcggcc agcacagggc ggcgcggggt ttgccggagg
     1441 cggaggcggc gggagcgggt tccggactcg gtttcatggg aggcgatcac gcttgcagga
     1501 cgtcggcgag cgccggcagg aagacttcgc agaccagcag cggccggccg tcgatccgga
     1561 aggtcgagcg ccggccccac agcggcaggc cgctggcacc ccgcacgtcg agcgcgcggc
     1621 gcgccagcgg gcagcgcagg tcgaggcgcg cgatctgcag cgggccgcgc acgacgccgg
     1681 gatcggcgaa cagtaccgcg ccgagcggac gcgtgccgag tccggtcagc gtgcggccgt
     1741 ggccgcgcag cgtgcgccgc gggatcacgg tgcgcgcgaa cacccacggc accccgccgg
     1801 cgagcaggtg cacctcgcgc acccaggcgg tgacgccgcg gcgcaggccg agcgcatcgg
     1861 cctcctcgcg cagcacgcgc ccggcgcgct cgctcagcac ccgcaccgag aaggcccggc
     1921 cgtggcgcgc gcattcggcg cgcagccgtg cggtcagcga accgcggccg agcaggcagt
     1981 cgcgcagccg ctcgcccatc tcggcgcgcc gcagccgcgc agccggctgc cagcgcgact
     2041 gcagcgtgaa gtcccggaag ctggcgggcg cctgcgccac ggcgcgaggc ccggaagcgg
     2101 caggaccgcc ggggaccggc acttgcgtca gcagccgcga cggccgcacc ggcgccgggg
     2161 ccgcaccgcc cgcccgccgc ccgtccgtcg tctcccctgc cctcacgccg cccgtctccc
     2221 gcagcatccc tcagagtccg ttcagcggat cgtccggatc gacgccgggc atgaacggca
     2281 cggcccgctc gtggccggtg atctggtaca tcaggccgag ccagttgttg aagatcgcct
     2341 tggcggtgtc ggcccaggtg ttgtcgagca ggcgcagcac cgcggcttcc gggaagtcgc
     2401 tgccgtcggg ctggcggccg tggtcctgct gctggaagac gtggttcttg tactcgtaga
     2461 gcagcgtctg cgcggcctcg gggaagtaat gctgcaccag cggcggatag tcggcgcgtt
     2521 cgccgtgcag gtagcgcagc acctcgcgct tgtattcctt cagcaggctg tcgatgtcgt
     2581 actccggatg cccctggaag aacacctgcc ggaagccgtc ggggctgacc accagatgcg
     2641 ggccgacctc ggcgctctcg accagcgtga ccagcccggc gtcatcgagc tgctcgcggg
     2701 tgatctcgtt ccagcgcgag tgcggcacgt cgaagcgcgt gttgatgttg cgcaccagcg
     2761 gatgccggcg atcgacgacg cggtgcggaa acacgcccca ggccttgtcc ggcagccggc
     2821 ggcggtgcac gccgtacttc atcagcatgg tcgcgtgcgc ggccagacac gcgaacagcg
     2881 tggtcgtgac ctgctcgtgc gcccattcga ccacctcgga gagcggcccc cagaacggtt
     2941 cctgcgtgaa gtcggggttg gagatgttgg cgccggtgat gatcagcgca tccaggccct
     3001 gcgcccggat ggtgtcgaag gactcgtacc agcgcccgat gtaggcctcg gcctcggcgc
     3061 cgcgcccgat gccgccgacc gtgaacggat gcacgtagaa ctgcgcgatg cggttgctcg
     3121 aaccgatcag gcgcatgaac tggcgctcgg tcgcggcgag cgcggcgtcc ggcatcatgt
     3181 tcagcaggcc gatgtgcagt tcgcggatgt cctgctgcgc ggcgcgcgcc tgcgcgagga
     3241 tttcctcgcc ttcctcggcg agccgcccga agctgggcag gccggaatgg gcaacgagtg
     3301 gcatggtgat gcggtctctg cggatcgtgg gttcggctgc acggcggatc gcagcgcagc
     3361 cgccggttgt gacaggggcg cgcaggatgg gtgcggttca gacctcgccg tagcgctcgc
     3421 gctcgccggt ccagcgacgg gccagcgcgg cggcggcgtt cgggtgttcg acgagcaggc
     3481 ggtcggcggc gcgctgcacg gccggcagca ggggctcgtc gcgcagcagg tcggcgatgc
     3541 gcagcgccag cgtgccggtc tggcgggtgc cgagcacccc gccggggccg cgcagttcga
     3601 ggtcgcgccg cgcgatcacg aagccgtcac tggtttcgcg cagcacgccg agccgcgccc
     3661 gaccctgctc ggacagcggc ggatggtaca tcagcacgca gctcgatgcc gcagcccccc
     3721 gcccgacccg gccgcgcagc tggtgcagct gcgcaagccc cagacgctcg gcgttctcga
     3781 tcaccatcac gctggcgttc ggcacgtcga cgccgacctc gatgaccgtc gtcgcgacca
     3841 gc
//
LOCUS       DAOQJH010000197         3836 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_14736_length_3836_cov_4.380323, whole genome shotgun sequence.
ACCESSION   DAOQJH010000197 DAOQJH010000000
VERSION     DAOQJH010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3836)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3836)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3836
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14736_length_3836_cov_4.380323"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(4..672)
                     /locus_tag="PLQ18_05705"
     CDS             complement(4..672)
                     /locus_tag="PLQ18_05705"
                     /inference="COORDINATES: protein motif:HMM:NF015642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein"
                     /protein_id="HNE01182.1"
                     /translation="MPESHAALALDDDERAELDAVLVAGGHDLEYADGLFCALICGPA
                     AVDRTCWLTLLFPVPVTGDAAARVLELVERHWNDIAHRLSLTREALDEDSFYLPFTHV
                     RSGAPGEALTGQRWAEGFRAGMAMAPDAWRPLLADEQVLAQLAPIVSLARPAGDARFG
                     AAADREALTDLLPLSAYRLARQLAVRRRTPLRVERPGRNDPCPCGSGRKYKHCCGAGR
                     PVTH"
     gene            complement(665..1954)
                     /locus_tag="PLQ18_05710"
     CDS             complement(665..1954)
                     /locus_tag="PLQ18_05710"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019915402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HNE01183.1"
                     /translation="MTASVQETQAGADVRSYMQELGRRARIAARAMSRADTAAKNAAL
                     LAIAAQIEAALPALLEANAADLEAARAGGLGAALLDRLALGEKGIRGMAQGLREVAAQ
                     PDPVGEITDLKFRPSGIQVGRMRVPLGVIGIIYESRPNVTADAAALCLKAGNACILRG
                     GSEAARSNRAIAACIAAGLEAAGLPAEAVQVVATTDRAAVGELIRMEQYVDVIVPRGG
                     KSLIARIAAEARVPVIKHLDGICHVYVDDRADIGKAVRVAFNAKCRRYGICGAMETLL
                     VHEAVAPYVLPQLAELYGAAGVELRGCERTRQLLPAALAATEEDWSTEYLDAILSIRV
                     VDGLDQAIAHIEQYGSHHTDSIITEDYTRARRFLREVDSASVMVNASTQFADGGEYGL
                     GAEIGISTDKLHARGPVGVEGLTTQKYVVLGDGSTRA"
     gene            complement(2045..3073)
                     /gene="holA"
                     /locus_tag="PLQ18_05715"
     CDS             complement(2045..3073)
                     /gene="holA"
                     /locus_tag="PLQ18_05715"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008931204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HNE01184.1"
                     /translation="MRQRPEQLAATLARGLKPVYLIHGEEPLQALEAADAVRAAARAQ
                     GFSEREVLNADAGFDWNSLAAAAGTQSLFGERRLVELRIPSGKPGDAGARALRAWCSR
                     PPGDTVLMVASGRLDAQQMKSAWFTALDEAGVSVQTFALETRAFPEWLTRRALARGLK
                     LAPEAVALLATQTEGNLLAAAQELDKLWMLHGDAPLDAEALLAAVADSARFGIYDLAD
                     AALDGDPARCARILGSLRAEGEEAVLTLWALAREIRTLLQAAQECARGSAPEAALTRL
                     KVWDKRKPLMRRALARLDAARLQRLLADCARVDRTVKGQEAGEPWDALLALALGLAGC
                     DAGLARSA"
     gene            complement(3088..3615)
                     /gene="lptE"
                     /locus_tag="PLQ18_05720"
     CDS             complement(3088..3615)
                     /gene="lptE"
                     /locus_tag="PLQ18_05720"
                     /inference="COORDINATES: protein motif:HMM:NF016290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HNE01185.1"
                     /translation="MASVLRIAILKAVFVMLLAGLSGCGFQLRGQLALPPVLERTHLQ
                     VAAGERSTLYQHLKAGLQVNGVSLVSDRRSATAVVEVGSESVSKQTVVKSGSGSALEY
                     ELFYSVDFRVTGADNKVLISQQNVRLSRDLLYDNATVIGRDTGETRATEDMRKDAAQA
                     ILRRIAALSSAQPAR"
     gene            complement(3618..>3836)
                     /locus_tag="PLQ18_05725"
     CDS             complement(3618..>3836)
                     /locus_tag="PLQ18_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE01186.1"
                     /translation="PQPDAGALARDSLTLAVQVNGKLRAQIEVAADADRLTIETAALA
                     DAQVQRFLEGRPAKKVIVVPGKLVNVVG"
BASE COUNT          505 a         1418 c         1384 g          529 t
ORIGIN      
        1 ccttcagtgc gtcacgggcc ggccggcccc gcagcagtgc ttgtacttgc gcccgctgcc
       61 gcacgggcag ggatcgttgc ggccgggccg ctcgacgcgc agcggcgtgc gccggcggac
      121 ggcgagctgg cgcgcgagcc ggtaggccga gagcggcagc aggtcggtca gcgcctcgcg
      181 atcggccgcc gccccgaaac gcgcatcgcc ggccgggcgc gccagcgaca cgatcggcgc
      241 gagctgcgcg agcacctgct catcggcgag cagcggccgc caggcatccg gcgccatcgc
      301 catgccggcg cgaaagccct cggcccagcg ctggccggtc agcgcctcgc ctggcgcgcc
      361 gctgcgcacg tgcgtgaacg gcagatagaa gctgtcctcg tcgagcgcct cgcgcgtgag
      421 cgacagccgg tgcgcgatgt cgttccagtg gcgctcgacg agttccagca cgcgcgcggc
      481 ggcgtccccg gtgaccggca ccgggaacag cagcgtcagc cagcaggtgc gatcgaccgc
      541 ggccggaccg cagatcagcg cgcagaacag gccgtcggcg tattcgagat cgtgcccgcc
      601 ggcgacgagc accgcgtcga gttcggcgcg ctcgtcgtca tcgagcgcca gcgcggcgtg
      661 cgactcaggc acgggtgctg ccatccccga gcacgacgta cttctgcgtg gtcaggccct
      721 cgacgcccac ggggccgcgg gcgtgcagct tgtcggtcga gatgccgatc tcggcaccga
      781 gcccgtactc gccgccatcg gcgaactgcg tcgaggcgtt gaccatgacc gaagccgaat
      841 cgacctcgcg caggaagcgc cgggcgcgcg tgtagtcctc ggtgatgatg ctgtcggtgt
      901 ggtgcgagcc gtactgctcg atgtgcgcga tcgcctggtc gaggccgtcg acgacgcgga
      961 tcgacaggat cgcgtcgagg tattcggtcg accagtcctc ctcggtcgcc gcgagcgcgg
     1021 ccggcagcaa ctggcgcgtg cgctcgcagc cgcgcagctc gacgccggcg gcgccgtaga
     1081 gctcggcgag ctgcggcagc acgtacggcg cgaccgcctc gtgcacgagc agggtttcca
     1141 tcgcgccgca gatgccgtag cgccggcact tggcgttgaa ggcgacgcgc acggccttgc
     1201 cgatgtcggc gcgatcgtcg acgtagacgt ggcagatgcc gtcgaggtgc ttgatcaccg
     1261 gcacgcgcgc ctcggcggcg atgcgcgcga tcaggctctt gccgccgcgc ggcacgatga
     1321 catcgacgta ctgctccatg cggatcagct cgccgaccgc ggcgcggtcg gtggtcgcga
     1381 ccacctgcac ggcctcggcc ggcaggccgg cggcttcgag gccggcggcg atgcaggcgg
     1441 cgatcgcgcg gttcgagcgc gccgcctcgg agccgccgcg caggatgcag gcgttgccgg
     1501 ccttcaggca cagcgccgcg gcatcggcgg tcacgttcgg acgcgattca taaatgatgc
     1561 cgatgacgcc gagcggcacg cgcatgcgcc cgacctggat gcccgagggg cggaacttca
     1621 ggtcggtgat ctcgccgacc ggatcgggct gggcggcaac ctcgcgcagg ccctgggcca
     1681 tgccgcggat gcccttctcg ccgagcgcga gccgatcgag cagcgccgcg ccgaggccgc
     1741 cggcgcgcgc ggcctcgaga tcggcggcgt tggcttcgag cagcgccggc agcgcggcct
     1801 cgatctgcgc agcgatcgcg agcagcgcgg cgttcttcgc ggcggtgtcg gcacggctca
     1861 tcgcccgcgc cgcgatgcgc gcgcgccggc cgagttcctg catgtagctg cgcacgtcgg
     1921 cgccggcctg ggtttcctgc acgcttgcag tcatcgtctt cacctcgaag cgcagccgct
     1981 gcgggcgact gcctgaacat tccgtccggc cggaaagcgg ccgggtctgc cgggatatgg
     2041 gggctcaggc gctgcgcgcc agcccggcgt cgcagccggc cagcccgagc gcgagcgcga
     2101 gcagcgcatc ccagggctcg ccggcctcct ggcccttgac cgtgcgatcg acgcgcgcgc
     2161 agtcggcgag cagccgctgc agccgtgccg catcgagccg cgccagcgcc cggcgcatca
     2221 gcggcttgcg cttgtcccag accttcagcc gcgtcagcgc cgcctcgggc gcgctgccgc
     2281 gggcgcactc ctgcgcggcc tgcagcagcg tgcggatctc gcgcgccagc gcccagagcg
     2341 tcagcacggc ttcctcgccc tcggcgcgca ggctgccgag gatgcgcgcg cagcgcgccg
     2401 gatcgccatc gagcgcggca tcggcgaggt cgtagatgcc gaagcgcgcg ctgtcggcga
     2461 ccgcggccag cagcgcctcg gcatcgagcg gggcgtcgcc gtgcagcatc cacagcttgt
     2521 cgagctcctg cgcggcggcg agcaggttgc cctcggtctg cgtcgccagc agcgccacgg
     2581 cttccggcgc gagcttcagg ccgcgcgcca gggcccggcg cgtcagccac tccggaaacg
     2641 cccgcgtctc gagcgcgaag gtctgcacgc tgacgccggc ctcgtcgagt gccgtgaacc
     2701 aggccgactt catctgctgc gcgtccagcc tgccgctggc caccatcagc accgtatccc
     2761 cgggcgggcg gctgcaccac gcgcgcagcg cccgggcgcc ggcgtcgccg ggcttgccgc
     2821 tggggatgcg cagctccacc agccggcgct cgccgaacag cgactgcgtg ccggccgccg
     2881 ccgcgaggct gttccagtcg aagccggcat cggcattcag cacctcgcgc tcgctgaagc
     2941 cctgcgcacg cgccgcggcg cgcacggcgt cggcggcttc gagcgcctgc agcggctcct
     3001 cgccgtggat caggtacacc ggcttcagtc cgcgcgcgag cgtcgcggcg agctgctcgg
     3061 gacgctggcg catgcccgct cccgtgctca gcgcgccggc tgcgccgacg aaagcgccgc
     3121 aatgcgccgc aggatcgcct gcgcggcatc cttgcgcatg tcctcggtcg cgcgcgtctc
     3181 gccggtgtcg cggccgatca ccgtggcgtt gtcgtacagc aggtcgcggc tcaggcgtac
     3241 gttctgctgg ctgatcagca ccttgttatc ggcaccggtg acgcggaaat cgacgctgta
     3301 gaacagttcg tattcaagcg cgctgcccga gccgctcttg accaccgtct gcttgctgac
     3361 cgactcgctg ccgacctcga ccacggccgt cgccgagcgg cgatcgctga cgaggctgac
     3421 gccgttgacc tgcagcccgg ccttcaggtg ctggtacagc gtcgagcgct cgccggccgc
     3481 cacctgcaga tgcgtgcgtt cgagcaccgg cggcagcgcg agctggccgc gcagctggaa
     3541 gccgcagccg ctcagcccgg ccagcagcat gacgaagacc gccttcagga tcgcgatgcg
     3601 cagcacgctt gccatgttca gccgaccacg ttgacgagct tgccgggcac gacgatgacc
     3661 ttcttcgccg gacggccctc caggaagcgc tgcacctggg catcggcgag cgcggcggtc
     3721 tcgatcgtca ggcgatcggc atcggccgcc acctcgatct gcgcgcgcag cttgccgttg
     3781 acctgcacgg ccagcgtcag gctgtcacgc gcgagcgcgc cggcatcggg ctgcgg
//
LOCUS       DAOQJH010000198         3828 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_14778_length_3828_cov_4.321230, whole genome shotgun sequence.
ACCESSION   DAOQJH010000198 DAOQJH010000000
VERSION     DAOQJH010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3828)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3828)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3828
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14778_length_3828_cov_4.321230"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..726)
                     /locus_tag="PLQ18_05730"
     CDS             complement(<1..726)
                     /locus_tag="PLQ18_05730"
                     /inference="COORDINATES: protein motif:HMM:NF012861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HNE01187.1"
                     /translation="MVRAHDPRPGLFLLGCAALCGVLSGLVMVAFRLLIDGAQMRLLG
                     AGRLDDFAALAPLTRIALAGAGGAALGLLWARLPAATRAVGVAHVLERCAYHGARLPG
                     INALLQFLGAASCLICGHSVGREGPAIHIGATAGSLLAERAALSLDARRLLVACGTAG
                     GIAAAFNTPLAGVAFAVEVLLGLSALPNLTPVLLAAVVATAISRGFYGDHALLPAAPP
                     GFDALAQLPGLALQGALTGLLAVA"
     gene            982..2019
                     /gene="argC"
                     /locus_tag="PLQ18_05735"
     CDS             982..2019
                     /gene="argC"
                     /locus_tag="PLQ18_05735"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HNE01188.1"
                     /translation="MIKVGIVGGTGYTGVELLRLLAAHPGVELTAITSRSEKGLKVAD
                     MFPNLRGRVDLAFVEPSPAVLEACDAVFFATPNGTAMKMVPELLAAGVRVIDLAADFR
                     LGDPAEWQQWYGMAHACPELLAEAVYGLPEVNREAIRAARLVANPGCYPTAVQLGFLP
                     LIEAGLIDDAQLIADAKSGVSGAGRKAEVHTLLCEAGENFKAYGVGGHRHLPEIRQGL
                     ARAAGHAVGLTFVPHLTPMIRGIHATLYGRLKSGAGAGDLQALYESRYASEAFVDVLP
                     AGSHPETRSVRGANTCRIAVHRPQGGDTIVVLSVIDNLVKGAAGQAVQNLNLMFGQPE
                     TAGIDGIALLP"
     gene            2146..2484
                     /gene="erpA"
                     /locus_tag="PLQ18_05740"
     CDS             2146..2484
                     /gene="erpA"
                     /locus_tag="PLQ18_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012981816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HNE01189.1"
                     /translation="MSEESLLVFTDSAATKVRSLIEEENNPALMLRVFVSGGGCSGFQ
                     YGFTFDEEVQDGDTMVENCGVRLLVDPMSYQYLVGAEIDYTEGLEGAQFVIRNPNATT
                     TCGCGSSFTV"
     gene            2667..>3828
                     /gene="nhaA"
                     /locus_tag="PLQ18_05745"
     CDS             2667..>3828
                     /gene="nhaA"
                     /locus_tag="PLQ18_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003672730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HNE01190.1"
                     /translation="MLSALRHFLKLEAASGILLFLAAVLAMIVANSPLAPWYGQFLDL
                     PVAVRIGPLEIAKPLLLWINDGLMAVFFLLVGLELKREVLEGELADPRQVALPAIAAI
                     GGMIVPALVYCAFNSGDPVALRGWAIPAATDIAFALGILALLGSRVPLALKVFLVSIA
                     IFDDLGAIVIIALFYTQDLSMLALGISLACLPLLLLLNRCNVTTFTPYMLIGLLMWTA
                     LLKSGIHATLAGVVLALFVPMRDRKAPESSPLRRLEHDLHTGVAFGILPLFAFANAGI
                     SFAGVGLQALTHPVALGVAGGLLIGKPLGVYGASLLGLKLGIGRLPESVDRRALFGVA
                     VLCGIGFTMSLFIGSLAFPEDAHAELFAERFGIVAGSLVSGLIGFVLLRRALP"
BASE COUNT          528 a         1358 c         1331 g          611 t
ORIGIN      
        1 ggccacagcc agcaggccgg tcagcgcacc ctgcagcgcg agccccggaa gctgcgcgag
       61 ggcatcgaag cccggcgggg ccgcgggcag cagcgcgtga tcgccgtaaa agccgcgcga
      121 gatcgccgtc gccacgaccg cggcgagcag caccggcgtc aggttcggca gcgccgacag
      181 gccgagcagt acctcgacgg cgaaggcgac gccggcgagc ggcgtgttga aggccgcggc
      241 gatgccgccg gccgtgccgc aggcgaccag cagccggcgg gcatcgagcg acagcgccgc
      301 gcgctcggcg agcaggctgc cggcggtggc gccgatgtgg atcgccggcc cctcgcggcc
      361 gaccgaatgc ccgcagatca ggcagctcgc ggccccgagg aactgcagca gcgcgttgat
      421 ccccggcagc cgggcgccgt ggtatgcgca gcgctccagc acgtgcgcga caccgacagc
      481 ccgcgtcgct gccggcaacc gtgcccacag caggccgagt gcggcgccac cggccccggc
      541 gagcgcgatg cgcgtcagtg gcgccagcgc cgcgaagtcg tcgagccggc cggcgccgag
      601 cagtcgcatc tgggcgccgt cgatcagcag ccggaaggcg accatcacca gccccgacag
      661 cacgccgcag agcgccgcgc agccgagcag gaacaggccc ggccgcggat cgtgcgcacg
      721 taccacggcg ggcgggaggc tcggattcgg catggccgcg cagcatgcca cggtgcgcgc
      781 cggatacgcg cgcgtaacgg catggcgtta tgattgccgc cctttttgct ccggccaccg
      841 ggccgggcgg ggcggtgacg gcggcctgcg ggcgtcgcga ccggtgagcg ggtgtggctt
      901 ccggcacgca ccggttccgg attcctcccg gattcctccc ggcttcatct actgaatgtt
      961 tcggttacgg agtggcaggc catgatcaag gtcggcatcg tcggcggcac cgggtatacc
     1021 ggcgtcgaac ttctgcggct gctcgccgcg catcccgggg tcgagctgac cgcgatcacc
     1081 tcgcgatcgg aaaaaggcct gaaggtcgcc gacatgttcc cgaacctgcg cgggcgcgtc
     1141 gatctggcct tcgtcgaacc gtcgccggcc gtgctcgaag cctgtgacgc ggtgttcttc
     1201 gcgacgccga acggcacggc gatgaagatg gtgccggagc tgctcgcggc cggcgtccgg
     1261 gtcatcgacc tcgcagccga cttccgcctc ggggatccgg ccgaatggca gcagtggtac
     1321 ggcatggcgc acgcctgccc cgagctgctg gccgaggccg tctacggcct gcccgaggtc
     1381 aaccgcgagg cgatccgcgc agcacggctg gtggccaacc ccggctgcta tccgaccgcg
     1441 gtgcagcttg gcttcctgcc gctgatcgag gccgggctga tcgacgatgc gcagctgatc
     1501 gccgatgcca agtccggcgt gtccggcgcc gggcgcaagg ccgaggtgca cacgctgctc
     1561 tgcgaggcgg gcgagaactt caaggcctac ggcgtcggcg gccaccgcca cctgcccgag
     1621 atccgccagg gcctcgcgcg cgccgccggc cacgccgtcg gcctgacctt cgtgccgcac
     1681 ctgacgccga tgatccgcgg catccacgcg acgctgtacg ggcggctgaa atccggtgcc
     1741 ggtgccggcg acctgcaggc gctgtacgag tcccgctacg cgagcgaggc cttcgtcgac
     1801 gtgctgccgg ccggctcgca cccggagacg cgctcggtgc gcggcgccaa cacctgccgg
     1861 atcgccgtgc accggccgca gggcggcgac accatcgtcg tgctgtcggt catcgacaat
     1921 ctggtcaagg gcgcggccgg tcaggccgtg cagaacctga acctgatgtt cggccagccc
     1981 gaaaccgccg gcatcgacgg catcgcgctg ctgccgtgac cacaagggcc ccctggcccg
     2041 ccggcgtgcg gatcgggtat tgttgatcgc tttgctcggg aaccccataa tccggtgcat
     2101 tcccgatttt tcgcagcgca cagcctgcgc gaggagtacc cggccatgtc cgaagaatcc
     2161 ctgctcgtgt tcaccgactc ggcggcgacc aaggtccgct cgctgatcga ggaagaaaac
     2221 aatccggccc tgatgctgcg cgtgttcgtc agcggcggcg gctgttcggg cttccagtac
     2281 ggcttcacgt tcgacgagga agtccaggac ggcgacacga tggtcgagaa ctgcggcgta
     2341 cgcctgctcg tcgatccgat gagctaccag tatctggtcg gcgccgagat cgactacacc
     2401 gagggcctcg aaggcgcgca gttcgtgatc cgcaacccga acgccacgac cacctgcggc
     2461 tgcggatcgt cgttcacggt ctgagcacgc gcggtttttc gtccttcccc gtttttcgtc
     2521 ttcccacgaa gaggccgcaa ggcctcttcg tcgtttccgg caagcggcgc gggcgggcgt
     2581 gctaaggttc gggccgactg cgcccttccc ttgcccgccg cggcaggccg cacggcctgc
     2641 cgccgcccga ccggagacgc cgcccgatgc tcagcgccct cagacacttc ctgaaactcg
     2701 aagccgccag cggcatcctg ctgttccttg ccgcggtgct ggcgatgatc gtcgccaatt
     2761 cgccgcttgc gccctggtac gggcaattcc tcgacctgcc ggtggccgtg cgcatcgggc
     2821 cgctggagat cgccaagccg ctgctgctgt ggatcaacga cggcctgatg gccgtgttct
     2881 tcctgctggt cggactggaa ctcaaacgcg aggtgctcga aggcgagctc gccgatccgc
     2941 gtcaggtcgc gctgccggcg attgcggcga tcggcggcat gatcgtgccg gcgctggtct
     3001 actgcgcgtt caactccggc gatccggtgg cgctcagggg ctgggcgatc ccggcggcga
     3061 ccgacatcgc cttcgcgctc ggcatcctgg cgctgctcgg ctcgcgcgtg ccgctggcgc
     3121 tgaaggtgtt cctggtgtcg atcgcgatct tcgacgatct cggtgcgatc gtgatcattg
     3181 cgctgttcta cacgcaggat ctgtcgatgc tggcgctcgg catctcgctc gcgtgcctgc
     3241 cgctgctgct gctgctgaac cgctgcaatg tcacgacctt cacgccctac atgctgatcg
     3301 ggctgctgat gtggacggcg ctgctgaaat ccggcatcca cgcgacgctc gccggcgtgg
     3361 tgctggcgct gttcgtgccg atgcgcgatc gcaaggcgcc cgagtcctcg ccgctgcgcc
     3421 ggctcgaaca cgacctgcac accggggtcg ccttcggcat cctgccgctg ttcgcgttcg
     3481 caaacgccgg catctcgttc gccggcgtcg gcctgcaggc gctgacgcac ccggttgcgc
     3541 tcggtgttgc cggcggcctg ctgatcggca agccgctcgg cgtctacggc gccagcctgc
     3601 tcgggctgaa gctcggcatc ggccgcctgc ccgagagcgt cgaccggcgg gcgctgttcg
     3661 gcgtcgcggt gctctgcggc atcggcttca cgatgagcct gttcatcggc tcgctggctt
     3721 tcccggagga tgcgcacgcc gaactgttcg ccgagcgctt cggcatcgtc gccggttcgc
     3781 tggtctcggg cctgatcggc ttcgtgctgc tgcgccgggc gctgccgg
//
LOCUS       DAOQJH010000199         3821 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_14807_length_3821_cov_4.077270, whole genome shotgun sequence.
ACCESSION   DAOQJH010000199 DAOQJH010000000
VERSION     DAOQJH010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3821)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3821)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3821
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14807_length_3821_cov_4.077270"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..239
                     /locus_tag="PLQ18_05750"
     CDS             <1..239
                     /locus_tag="PLQ18_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNE01191.1"
                     /translation="LLGEGAYFAPELGNVYKRFGNSTEAIKAFIRSRPENGVPGRRSM
                     PQFHLSDAELEGIAQFLKYSSEINTANWPPNIQG"
     gene            271..1677
                     /locus_tag="PLQ18_05755"
     CDS             271..1677
                     /locus_tag="PLQ18_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HNE01192.1"
                     /translation="MQYLSQKVAKPYFVAAIGLFAGQILFGLIMGIQYLVGDFLFPAI
                     PFNVARMVHTNLLIVWLLFGFMGASYYLVPEEAERELESPLLAKILFWVFLIAGALTV
                     VGYLAVPYASLAKLTGNDVLPTMGREFLEQPTITKVGIVVVALGFLYNVGLTILKGRK
                     TVINLVLLTGLAGLAVLFLFSFYNPDNLVLDKFYWWWVVHLWVEGVWELILGSILAFV
                     LIKVTGVDREIIEKWLYVIIAMALITGIIGTGHHYFWIGTPEYWQWLGSVFSAMEPLP
                     FFAMTVFAFNMVNRRRREHPNQAATLWALGTSVMAFLGAGVWGFLHTLAPVNYYTHGT
                     QITAAHGHMAFYGAYVMIVLTIISYAMPMLRGQTAANSRRSQVLEMWSFWLMTVSMVF
                     ITLFLTGAGILQVYLQRYSAQPLPFMVVQEQISLFYWLRLIAGLVFTIGLGAYIASFF
                     VGRTEPEAVAMKSVPVTA"
     gene            1813..2595
                     /locus_tag="PLQ18_05760"
     CDS             1813..2595
                     /locus_tag="PLQ18_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005961374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbbQ/NirQ/NorQ/GpvN family protein"
                     /protein_id="HNE01193.1"
                     /translation="MSELPYYAPAGNEIALFEAAWRERLPVLLKGPTGCGKTRFVAHM
                     AARLGRPLYTVACHDDLTAADLVGRHLIGDGGTWWSDGPLTRAVREGAICYLDEVVEA
                     RKDTTVVLHPLADDRRILPIERTGETLAAPAEFMLVVSYNPGYQNLLKGLKPSTRQRF
                     VAMAFDYPDAAREREILMREAGIDAGLAERLVRLGHGLRALKDHDLDEGASTRLLIYA
                     ATLMRAGLAPRAACRAALIEPLTDDTATLLALQEVVDVAFPG"
     gene            2576..2974
                     /locus_tag="PLQ18_05765"
     CDS             2576..2974
                     /locus_tag="PLQ18_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018077753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE01194.1"
                     /translation="MSRFPAERRPDADGRRLAVRAQALYLANLLILPGLAFAALLWLR
                     RRRAGVPLLAQAHLDAALHASLWALALLPGVAGLLVLVAGLRAPATWMTLILWVVSMH
                     TALVLWGLLALAAALAGRLPPPPWRGRISG"
     gene            3279..>3821
                     /locus_tag="PLQ18_05770"
     CDS             3279..>3821
                     /locus_tag="PLQ18_05770"
                     /inference="COORDINATES: protein motif:HMM:NF017081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HNE01195.1"
                     /translation="MNAAPLTVPPAPLTAALLLAAAYVLAGAAGLALALPPDTEIAVW
                     PAAGVAAGGLLAGGLRLWPAVWIGSFVINLWLRLSLLGQPPTASAFLIPAGIACGAAA
                     QAWVGARLSAALLARPHPLAHGTETLSFLLRVGPLACLISATTATLLLVASASVPVEA
                     AGGLFWTWWCGDTLGVLLFTP"
BASE COUNT          491 a         1383 c         1269 g          678 t
ORIGIN      
        1 cgctgctcgg cgagggtgcg tatttcgcgc cggaactcgg caacgtctac aagcgcttcg
       61 gcaacagcac cgaggcgatc aaggccttca tccggagccg gccggaaaac ggtgtgccgg
      121 gccggcgctc gatgccgcag ttccacctga gcgacgccga actcgaaggc atcgcgcaat
      181 tcctgaagta cagctccgaa atcaacaccg ccaactggcc gccgaacatc cagggctgat
      241 ccgcctgcgc ccgcccgagg acatcacgtc atgcaatacc tgtcgcaaaa ggtcgcgaaa
      301 ccgtatttcg tcgccgcgat cgggctgttc gccggccaga tcctgttcgg cctgatcatg
      361 ggcatccagt atctggtcgg cgacttcctg ttcccggcca ttccgttcaa cgtggcccgc
      421 atggtccaca ccaatctgct gatcgtctgg ctgctgttcg gcttcatggg cgcgagctat
      481 tacctcgtgc ccgaggaggc cgagcgcgag ctcgaaagcc cgctgctcgc gaagatcctg
      541 ttctgggtgt tcctgatcgc cggcgcgctg accgtcgtcg gttatctggc cgtgccgtat
      601 gcgtcgctcg cgaagctgac cggcaatgac gtgctgccga ccatgggccg cgaattcctc
      661 gaacagccga ccatcaccaa ggtcggcatc gtcgtggtcg cgctcggttt cctctacaac
      721 gtcggcctga ccatcctcaa gggccgcaag accgtcatca acctggtgct gctgaccgga
      781 ctcgccgggc tggccgtgct gttcctgttc tcgttctaca accccgataa cctggtgctc
      841 gacaagttct actggtggtg ggtcgtgcac ctgtgggtcg agggcgtctg ggagctgatc
      901 ctcggttcga tcctcgcctt cgtgctgatc aaggtcaccg gcgtcgatcg cgagatcatc
      961 gagaagtggc tgtacgtgat catcgcgatg gcgctgatca ccggcatcat cggcaccggc
     1021 caccactact tctggatcgg cacgccggag tactggcagt ggctcggctc ggtgttctcg
     1081 gcgatggaac cgctgccgtt cttcgcgatg acggtgttcg cgttcaacat ggtcaaccgc
     1141 cgccgccgcg agcacccgaa ccaggccgcg acgctgtggg cgctcggcac ctcggtgatg
     1201 gctttcctcg gcgccggggt ctggggtttc ctgcacacgc tcgcgccggt caactactac
     1261 acgcacggca cgcagatcac cgccgcgcac ggccacatgg ccttctacgg cgcctacgtg
     1321 atgatcgtgc tgaccatcat cagctacgcg atgccgatgc tgcgcggcca gaccgccgcc
     1381 aacagccgcc gctcgcaggt gctggagatg tggtcgttct ggctgatgac ggtgtcgatg
     1441 gtgttcatca cgctgttcct gaccggcgcc ggcatcctgc aggtctacct gcagcgctac
     1501 agcgcccagc cgctgccgtt catggtcgtg caggagcaga tcagcctgtt ctactggctg
     1561 cggctgatcg ccggcctggt gttcaccatc ggcctcggtg cctacatcgc gagcttcttc
     1621 gtcgggcgca ccgagccgga agcggtggcg atgaagtcgg taccggtgac ggcctgaagc
     1681 cccggccggc cccgggcgtg agggacgacc gtccctcacg cctttttcat ccgggtcccg
     1741 ggtgccgcgc ggatggtcct ttggctgcgc ggtgctcccc tgccctttcc cctgccccgt
     1801 cccggagccg ccatgtccga gcttccgtat tacgccccgg ccggcaacga gatcgcgctg
     1861 ttcgaggccg cctggcgcga gcggctgccg gtgctgctca agggcccgac cggctgcggc
     1921 aagacgcgct tcgtcgcaca catggccgcc cgcctcggcc ggccgctgta tacggtcgcc
     1981 tgccacgacg acctgaccgc cgccgatctg gtcggccggc acctgatcgg cgacggcggc
     2041 acctggtgga gcgacgggcc gctgacgcgc gcggtgcgcg agggtgcgat ctgctacctc
     2101 gacgaagtcg tcgaggcgcg caaggacacc accgtcgtgc tgcacccgct ggccgacgac
     2161 cggcgcatcc tgccgatcga gcgcaccggc gaaaccctcg ccgcaccggc ggaattcatg
     2221 ctcgtggtct catacaaccc cggctaccag aacctgctga agggcctgaa gccctcgacc
     2281 cgccagcgct tcgtggcgat ggccttcgac tacccggacg cggcacgcga gcgcgaaatc
     2341 ctgatgcgcg aggccggcat cgacgccggg ctggccgagc gcctcgtgcg cctcggccac
     2401 ggcctgcgtg cgctgaagga tcacgacctc gacgagggcg cctcgacgcg gctgctgatc
     2461 tacgccgcga cgctgatgcg cgccggcctc gcgccgcgcg ctgcgtgccg ggcggcgctc
     2521 atcgaaccgc tgaccgatga cacggccacg ctgctcgcgc tgcaggaagt cgtcgatgtc
     2581 gcgtttcccg gctgagcgca ggcccgatgc cgacggccgc cggctggcgg tgcgcgcgca
     2641 ggcgctgtac ctcgccaacc tgctgatcct gccgggcctc gcgttcgcgg cgctgctgtg
     2701 gctgcgccgc cggcgtgccg gtgtgccgct gctcgcgcaa gcgcacctcg atgcggcgct
     2761 gcacgcgagc ctctgggcac tggccctgct gccgggcgtg gccgggctgc tggtgctggt
     2821 cgccggtctg cgtgcgccgg cgacctggat gacgctgatc ctgtgggtgg tcagcatgca
     2881 cacggcactg gtgctctggg ggctgctggc gctggccgcg gccctggcag gacggctgcc
     2941 gccgccgccg tggcgcggcc gcatcagcgg gtgagcgcga tcacgcgcga acggcctgtc
     3001 ctgcccgtcc tgtctctgcc tatccttctc ccgcgcccgg ccgggccctg ccgagccggt
     3061 gcgtgcggcc gtgagcggca cgaaacggct gcaacaagcg atttcatccg gcattgaggt
     3121 tttccggtac cgtcgcgctg ccgcgcgcag cggatcccgg cattcgcgaa cgctgacttt
     3181 gcggctagcc ttgcgcgcgg catgtccgcc ccgccccgaa cccgctcttc tggtccggca
     3241 tgccgtttcc ggccccagtg ctgccgtcgg tttcgtcgat gaacgccgcc cccctcactg
     3301 taccgcccgc ccccctgacg gcagccctgc tgcttgccgc agcctatgtg cttgccggcg
     3361 cggccgggct ggcgctggcg ctgccgcccg ataccgagat cgccgtctgg ccggcggccg
     3421 gtgtcgccgc cggcggcctg ctcgccggcg gcctgcggct gtggccggcg gtgtggatcg
     3481 gctccttcgt gatcaacctg tggctgcgcc tgagcctgct cggccagccg ccgacggcct
     3541 cggcgttcct gattccggcc ggcatcgcct gcggggccgc cgcgcaggcc tgggtcggcg
     3601 cgcggctgag cgcggcgctg ctcgcgcgcc cgcacccgct cgcgcacggc accgagacgc
     3661 tgtccttcct gctgcgcgtc ggcccgctcg cctgcctgat cagcgccacg accgccacgc
     3721 tcctgctggt cgccagcgcc tcggtgccgg tcgaggccgc gggcgggctg ttctggacct
     3781 ggtggtgcgg cgatacgctc ggcgtgctgc tgttcacgcc g
//
LOCUS       DAOQJH010000200         3816 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_14838_length_3816_cov_3.553576, whole genome shotgun sequence.
ACCESSION   DAOQJH010000200 DAOQJH010000000
VERSION     DAOQJH010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3816)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3816)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3816
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14838_length_3816_cov_3.553576"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="PLQ18_05775"
     CDS             <1..262
                     /locus_tag="PLQ18_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016403352.1"
                     /note="universal genome maintenance protein;
                     Kae1/Qri7/OSGEP/YgjD family protein; in Archaea, some Kae1
                     are found as fusion proteins similar to two distinct
                     proteins in yeast that are involved in the KEOPS complex;
                     kinase associated endopeptidase 1 (Kae1) and a
                     serine/threonine protein kinase (Bud32); in Pyrococcus
                     Kae1 has atypical AP endonuclease activity and inhibits
                     the kinase activity of Bud32; the protein is essential in
                     Escherichia coli and Bacillus subtilis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HNE01196.1"
                     /translation="RETGLKRLVVAGGVGANERLRAQLAALMQARGGRAYYPRPAFCT
                     DNGAMIAYAGHCRLAAGEREDGPIRAVPRWPLDALQPPPALA"
     gene            complement(274..873)
                     /gene="plsY"
                     /locus_tag="PLQ18_05780"
     CDS             complement(274..873)
                     /gene="plsY"
                     /locus_tag="PLQ18_05780"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007041210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HNE01197.1"
                     /translation="MTSALLCLVFAYLCGSVSTAILVCRLMGLPDPRSEGSRNPGATN
                     VLRIGGRKAAAITLAGDFLKWLIPVLVARAFGQGPEVLAATAFAAFLGHLYPVFFGFQ
                     GGKGVATALGVMVGLSWQVALAALATWLVIARVWKISSLAALGAATLSPLYALLFGLP
                     GAYALALLPMVALLLWRHRENIRRIRAGTESRIGDKKKT"
     gene            1031..1390
                     /gene="folB"
                     /locus_tag="PLQ18_05785"
     CDS             1031..1390
                     /gene="folB"
                     /locus_tag="PLQ18_05785"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005960975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HNE01198.1"
                     /translation="MDIIFIRDLSIETTIGVYEWERGIRQTIVLDLELGTDVRRSAAS
                     GALEDALDYMAVATRLGAYVGGERWELVETLAERIAELLITEFHVPWLRLTLTKPAAV
                     RGARAVGVRIERSAGGG"
     gene            complement(1396..2088)
                     /locus_tag="PLQ18_05790"
     CDS             complement(1396..2088)
                     /locus_tag="PLQ18_05790"
                     /inference="COORDINATES: protein motif:HMM:TIGR04211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04211 family SH3 domain-containing protein"
                     /protein_id="HNE01199.1"
                     /translation="MRRPTLAALCLLCTLLTLLPVASAPAKTVRYVSDSLEVPLRAGA
                     SLRHRIVRMLPSGTQVEVLETDTDKGFALVRTADGTVQGWLPNQYLMETPSARTALGT
                     MQTEFDRVRSENASLKERLDTLTTQKVDAETSFSQLSSDNHRLAQELATIRRTAANAI
                     AIDQQNKDLQERVVNLERELQIVQQENQSLADRSNRDWFLLGAGVLVSGVLVGLILPR
                     LRVQRRSRWGEL"
     gene            complement(2151..2900)
                     /locus_tag="PLQ18_05795"
     CDS             complement(2151..2900)
                     /locus_tag="PLQ18_05795"
                     /EC_number="1.5.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002691103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pteridine reductase"
                     /protein_id="HNE01200.1"
                     /translation="MEDITGDARVALVTGAARRVGAEIVRTLHATGWRIALHYRSAAA
                     EAETLASELNARRTASVATFAADLLDTAALPGLVEAAATVWGRLDLLVNNASSFYATP
                     VGQATEAHWDDLVGSNLKAPFFLSQAAAPWLRAHEGCIVNIVDLHAGRPMDEHPIYCA
                     AKAGLASLTRSLARELAPEVRVNGIAPGAILWPEHSIDAGERERILGRVPLGRAGSPA
                     DIARTLLFLVQYAPYVTGQIIAVDGGRTTGV"
     gene            2969..>3816
                     /locus_tag="PLQ18_05800"
     CDS             2969..>3816
                     /locus_tag="PLQ18_05800"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015279559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HNE01201.1"
                     /translation="MRAFERSLADFPAPSSEALAVCARLVDQIAADIAAAGGRLRFDH
                     YMQRALYAPGLGYYAAGSRKFGAGGDFTTAPELSPLFSRCLARAGAPVLAALGGGDVL
                     EFGAGTGTMAAGVLAELARLDCLPGRYRILDASAELRERQRATLAAKVPALLDRVEWL
                     DALPPAGSLRGVVLGNELLDAMPVRRFRIGADGPQELYVRGDAATGFELVTGPADAGC
                     RAAVAAIEAAVGPLAEGYESEWNPALPAWFASLGETLAAGAAILIDYGHPRRAYYHPS
                     RARGHG"
BASE COUNT          522 a         1377 c         1388 g          529 t
ORIGIN      
        1 gcgcgagacc ggcctgaagc ggctggtggt ggccggcggc gtcggtgcca acgagcgcct
       61 gcgcgcgcag ctcgccgcgc tgatgcaggc gcgcggcggc cgggcctatt acccgcgccc
      121 ggccttctgc accgacaacg gcgcgatgat tgcctatgcc ggtcactgcc ggctcgcggc
      181 cggcgagcgc gaggacggcc cgatccgcgc cgtgccgcgc tggccgctcg atgcgctgca
      241 gccgccgccg gccctcgcgt agccggggcc ggatcaggtc ttcttcttgt cgccgatgcg
      301 gctttcggtg ccggcgcgga tgcggcggat gttctcgcgg tggcgccaca gcagcagcgc
      361 gaccatcggc agcagcgcca gcgcgtaggc cccgggcagg ccgaacagca gcgcgtacag
      421 cggcgacagc gtcgcggcgc cgagtgccgc gagcgaggaa atcttccaga cccgcgcgat
      481 caccagccag gtcgcgagcg cggccagcgc cacctgccac gacagcccga ccatgacgcc
      541 gagcgcggtc gccacgccct tgccgccctg gaagccgaag aacaccgggt acagatggcc
      601 gaggaaggcc gcgaacgccg tcgccgccag cacttccggc ccctgcccga aggcgcgcgc
      661 gaccagcacc gggatcagcc atttcaggaa gtcaccggcc agcgtgatcg cggcggcctt
      721 cctgccgccg atgcgcagca cgttggtcgc gccggggttg cgcgagccct ccgagcgcgg
      781 atcgggcagg cccatgagcc ggcagacgag gatcgcggtc gagaccgagc cgcacagata
      841 cgcgaacacg aggcagagca gggcgctggt catgggcgga gcaggagtcc tgggaggcgg
      901 cgggaagccg cagggaagga ggaacacgga cggcgatggc cggcgctcct gcggcggcga
      961 tgcgcggggg agcattggcg gtcgtttccg gcgatcatgg atcttctcgc aaaagccacc
     1021 gagccggcga atggacatca tcttcatccg cgacctgagc atcgagacca cgatcggcgt
     1081 ctacgaatgg gagcgcggca tccgccagac catcgtgctc gacctcgaac tcggcaccga
     1141 cgtgcgccgc agcgccgcca gcggtgcgct ggaggatgcg ctcgactaca tggcggtcgc
     1201 cacgcgcctc ggcgcctacg tcggcggcga gcgctgggaa ctcgtcgaga cgctcgccga
     1261 gcgcatcgcc gagctgctga tcacggagtt ccacgtgccg tggctgcggc tgacgctgac
     1321 caagccggcc gcggtgcgcg gtgcgcgcgc cgtcggcgtg cgcatcgagc gcagcgccgg
     1381 cggcggctga ttcgctcaga gctcgcccca gcggctgcgc cgctgcacgc gcagccgcgg
     1441 caggatcagc ccgaccagca cgcccgagac cagcacgccg gccccgagca ggaaccagtc
     1501 cctgttgctg cgatcggcca gcgactggtt ctcctgctgc acgatctgca gctcgcgctc
     1561 caggttgacg acgcgctcct gcaggtcctt gttctgctgg tcgatggcga tggcgttggc
     1621 ggcggtcctg cggatcgtcg ccagttcctg cgccagccgg tggttgtcgg aggacagctg
     1681 cgagaagctc gtctcggcat cgaccttctg cgtggtcagc gtgtcgaggc gttccttgag
     1741 gctggcgttc tcggagcgca cgcgatcgaa ctcggtctgc atggtgccga gggccgtgcg
     1801 cgccgacggc gtttccatca ggtactggtt cggcagccag ccctgcacgg tgccatcggc
     1861 cgtgcgcacc agcgcgaaac ccttgtcggt gtcggtctcc agcacctcga cctgcgtgcc
     1921 gctcggcagc atgcgcacga tccggtggcg caggctggcg ccggcgcgca gcggcacttc
     1981 gaggctgtca ctgacgtagc ggacggtctt cgctggcgcg gaggcgaccg gcagcagcgt
     2041 gagcagggtg cagagcaggc agagcgcggc gagcgtcgga cggcgcatcg ggggacttcc
     2101 tcacaacggg gcatgcggca cagagccggc gagtgtaccc cgacccggct tcagacgccg
     2161 gtcgtgcgcc cgccgtcgac ggcgatgatc tgcccggtca cgtagggcgc gtactgcacc
     2221 aggaacagca gcgtgcgcgc gatgtcggcc ggcgaaccgg cgcgcccgag cggcacgcgc
     2281 ccgaggatgc gctcgcgctc gccggcgtcg atcgagtgct ccggccacag gatcgcgccc
     2341 ggcgcgatgc cgttgacgcg cacctccggg gccagctcgc gtgccagcga gcgcgtcagc
     2401 gaggccagac ctgccttggc ggcgcagtag atcgggtgct cgtccatcgg ccggccggcg
     2461 tgcaggtcga cgatgttgac gatgcagccc tcgtgcgcgc gcagccacgg cgccgcggcc
     2521 tggctcagga agaacggtgc cttcaggttc gagccgacca gatcgtccca gtgcgcctcg
     2581 gtcgcctgcc cgaccggcgt cgcgtagaac gacgaggcgt tgttgaccag caggtcgagc
     2641 cggccccaga ccgtcgccgc cgcctcgacc agcccgggca gcgccgcggt gtcgagcagg
     2701 tcggcggcga aggtcgcgac cgaggccgtg cgccgggcgt tcagttcgga agcgagcgtc
     2761 tcggcctcgg cggcggcgct gcggtagtgc agcgcgatgc gccagccggt cgcgtgcagc
     2821 gtgcgcacga tctcggcgcc gacccggcgc gctgcgccgg tgaccagcgc gacgcgtgca
     2881 tccccggtga tatcctccac ggccctcgcc ccctcgcgca gtcggttgcg gctagcttcg
     2941 ccgacttgtg cccgctgcgg aaagctccat gcgcgccttc gaacgctccc tcgccgattt
     3001 tccggcgccc tcatccgagg cgctggctgt ctgcgcccgg ctggtcgatc agatcgccgc
     3061 cgacatcgcc gctgccggcg ggcggctgcg cttcgatcac tacatgcagc gcgcgctgta
     3121 cgcgcccggc ctcggttact acgccgccgg cagccgcaag ttcggcgccg gcggggattt
     3181 cacgaccgcg ccggaactct cgccgctgtt ctcgcgctgt ctggcgcgtg ccggcgcgcc
     3241 ggtgctcgcc gcactcggcg gtggcgacgt gctcgaattc ggcgccggca ccggcacgat
     3301 ggccgccggc gtgctcgccg aactggcgcg gctcgactgc ctgcccgggc gctaccgcat
     3361 cctcgatgcc agcgccgaat tgcgcgagcg ccagcgcgca acgctggccg cgaaggtgcc
     3421 ggcgctgctc gatcgcgtcg aatggctcga tgcactgccg ccggccggca gcctgcgcgg
     3481 cgtcgtgctc ggcaacgaac tgctcgatgc gatgccggtg cggcgctttc gcatcggcgc
     3541 cgacggcccg caggagctgt acgtccgcgg tgatgccgca accggcttcg aactggtcac
     3601 cggccccgcc gatgccggct gccgggccgc ggtcgcggcg atcgaggccg cggtcgggcc
     3661 gctcgccgag ggctacgaat cggaatggaa tccggcgctg ccggcgtggt ttgcgagcct
     3721 cggcgagacg ctcgccgccg gggccgcgat cctgatcgac tacggccacc cgcgccgcgc
     3781 ctattaccac ccgtcgcgcg cgcgcggtca cggcca
//
LOCUS       DAOQJH010000201         3815 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_14845_length_3815_cov_3.201862, whole genome shotgun sequence.
ACCESSION   DAOQJH010000201 DAOQJH010000000
VERSION     DAOQJH010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3815)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3815)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3815
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14845_length_3815_cov_3.201862"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..680)
                     /locus_tag="PLQ18_05805"
     CDS             complement(<1..680)
                     /locus_tag="PLQ18_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019143009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNE01202.1"
                     /translation="MPKSTRPAAPAPLPPRATVRLQLHRDFPFAAAAAQVPYLAALGI
                     SHVYASPILKARAGSTHGYDVVDPGCVNPELGGEDGLRALVATLRAHGMGLVVDIVPN
                     HMAVGGPENPYWLDVLEWGRASPYAEFFDIDWDATDPALRGRLLAPFLGAPYGEALDR
                     GELRLHFDAVSGRFSCAYFDNRFPIAPTRYPALLRLGGEALAAAARDFRAALAGRAGG
                     RRERFDAA"
     gene            complement(875..3010)
                     /gene="malQ"
                     /locus_tag="PLQ18_05810"
     CDS             complement(875..3010)
                     /gene="malQ"
                     /locus_tag="PLQ18_05810"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HNE01203.1"
                     /translation="MPHAPADLAALAQTAGLAAEWTDYRGIRHAIAPATQQQVLEALG
                     IDCTGAAAIADSRARLLAEAAAPPALAVLRSAEPWTLPAAFGTQPGAAVELVDEAGEC
                     VGTRLIADAAGRAALPGLLLDAGYYTLRLDGHAQALALAPAGAPGFARLAGRERGFGF
                     AAQLYGLRRGADGGVGDFSALAEHAAALGAHGASALAISPVHALFAADPQHYSPYSPS
                     NRQFLNYLHADAHAAGLGAEAAACDALGPSVPGTGGDFVDWPGVARARLAALRRLWDA
                     LAARLRDGDDALALDYRRFVEAGGEALERHARFELLHGLEFGADLQRWHWRSWPAALQ
                     QPDSPEVARLAHAHAHELGFHRFAQWLAARGLAAAQAAARAAGMEIGLITDIAVGTSS
                     GGSAAWSAPGEVFTGLSIGAPPDPLGPTGQTWGLVTHAPQALRSHGYAPFLQLLRAAL
                     RSAGGVRIDHILGLRRLWVVPDGENGAGGCYLDYPLDDLLALVALEAHRHRALIVGED
                     LGTVPPGLHERLAAHGIAGMSVLWFQRDHGLFVEPARWPATALAMTSTHDLPTVAGWW
                     QGRDLDWRTQVGHLEGAELVEAVAGRACDREALWAAFTHAGIAEGPMPEALAPAPVVD
                     AALAYTAATPAALALLPLEDLCGLGEQPNLPGTVHEHPNWCRRLPGTAEALLAEPAVA
                     ARLAMLRRLRGAGPAITAAHGEGQAPPAS"
     gene            complement(3023..>3815)
                     /locus_tag="PLQ18_05815"
     CDS             complement(3023..>3815)
                     /locus_tag="PLQ18_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014618538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3459 domain-containing protein"
                     /protein_id="HNE01204.1"
                     /translation="RAGELFVRCLGEGFGWQGEPSPGHDGRPRGTPSAHLPPSAFVFF
                     LQNHDQIGNRALGERLLALCAQAGRLEAWRAALAVQLLCPQVPLLFMGDELGSTTPFL
                     FFTDFHDELAEAVREGRRREFAQFAAFADPERRAAIPDPNAGTTFESSRPQPGDPEDA
                     ARVAMLLRLRNTLLAPRLAGTRALGSAALGPAALRARWRLGDGTMLTIYTNLADMPVE
                     LDFVPPAGSLLYESRDGAAAALARDDLAAAATTVWLSAGTVPDSP"
BASE COUNT          519 a         1394 c         1482 g          420 t
ORIGIN      
        1 caggcggcgt cgaagcgctc gcgccggccg ccggcgcggc cggccagcgc ggcccgaaag
       61 tcacgcgctg ccgcagccag cgcctcgccg ccgaggcgca gcagcgccgg atagcgggtg
      121 ggcgcgatcg ggaagcggtt gtcgaagtac gcgcacgaaa aacgcccgct gacggcatcg
      181 aagtgcaggc gcagctcgcc gcgatcgagc gcctcgccgt agggcgcgcc gaggaagggc
      241 gcgagcagcc ggccgcgcag cgccgggtcg gtggcatccc agtcgatgtc gaagaactcg
      301 gcatacgggc tggcgcggcc ccattcgagc acgtcgagcc agtacggatt ctccgggccg
      361 ccgaccgcca tgtgattcgg cacgatgtcg acgacgaggc ccatgccgtg cgcgcgcagc
      421 gtcgcgacca gcgcccgcag gccgtcctcg ccgccgagtt cggggttcac gcagccggga
      481 tcgaccacgt cgtagccgtg tgtcgagccg gcgcgcgcct tcaggatcgg gctggcgtag
      541 acgtggctga tgccgagcgc ggccagatac ggcacctgtg cggcggcggc cgcgaacggg
      601 aagtcccggt gcagctgcag gcgcacggtc gcgcgtggcg gcagcggagc cggagcggcg
      661 gggcgggtgg atttcggcat cgggcgtcgc ctcgctcggg gggaggatcg gggtgcgcgg
      721 gtccggaccc gcgcggcgga ttccgatgcc ccggcccgcg gatgcggacg aggcactcag
      781 cagccgacgc cggcgccggg caacggttcc cggcccgcgc ccgacgcttc acccggggcc
      841 ccgaagcccg gcgcgccggg aacgcagctc aggatcagga cgccggcgga gcctgcccct
      901 cgccgtgcgc cgccgtgatt gcggggccgg ccccgcgcag ccgccgcagc attgccagac
      961 gcgcggcgac cgccggttcg gcgagcaggg cttcggccgt gcccggcagg cgccggcacc
     1021 agttcgggtg ctcatgcacc gtgcccggca ggttcggctg ttcgccgagg ccgcagaggt
     1081 cttcgagcgg cagcagcgcg agcgcggccg gcgtcgcggc ggtgtaggcg agcgcggcat
     1141 cgacgaccgg cgccggggcc agcgcttcgg gcatcgggcc ttcggcgatg ccggcgtgcg
     1201 tgaacgccgc ccacagcgcc tcgcgatcgc acgcgcgccc ggcgaccgcc tcgaccagct
     1261 ccgcaccttc gaggtggccg acctgcgtgc gccagtcgag gtccctgccc tgccaccagc
     1321 cggcgacggt cggcagatcg tgcgtcgagg tcatcgccag cgccgttgcc ggccagcggg
     1381 cgggctcgac gaacaggccg tggtcgcgct ggaaccacag caccgacatg ccggcgatcc
     1441 catgcgcggc cagacgctcg tgcaggccgg gcggcacggt gccgaggtcc tcgccgacga
     1501 tcagcgcccg gtggcggtgc gcctccagcg ccaccagcgc cagcaggtca tcgagcggat
     1561 agtcgaggta gcagccgccg gcgccgttct cgccgtccgg cacgacccac agccgccgca
     1621 gcccgaggat gtggtcgatg cgcacgccgc cggccgagcg cagcgccgcg cgcaaaagct
     1681 gcaggaacgg cgcatagccg tggctgcgca gtgcctgcgg cgcatgcgtg accaggcccc
     1741 aggtctggcc ggtcgggccg agcggatcgg gcggtgcgcc gatcgacagc cccgtgaaca
     1801 cctcgcccgg agccgaccac gccgcgctgc cgccgctgct ggtgccgacg gcgatgtcgg
     1861 tgatcaggcc gatttccatg ccggcggcgc gcgccgcggc ctgcgccgcc gccagcccgc
     1921 gcgccgccag ccactgcgcg aagcggtgga agccgagttc atgggcatgc gcatgcgcaa
     1981 gccgcgcgac ctcgggcgaa tccggctgct gcagggctgc cggccagctg cgccagtgcc
     2041 agcgctgcag gtcggcgccg aattcgagac cgtgcagcag ctcgaagcgc gcatggcgtt
     2101 cgagcgcctc gccgccggcc tcgacgaagc ggcggtaatc gagtgcgagt gcgtcatcgc
     2161 cgtcgcgaag gcgcgcggcc agcgcatccc agagccggcg cagcgccgcc agccgtgcgc
     2221 gcgcgacccc gggccagtcg acgaaatccc cgccggtgcc gggcacggac gggccgagcg
     2281 catcgcaggc cgcggcctcg gcgccgaggc cggcggcgtg cgcatcggcg tgcaggtagt
     2341 tcaggaactg gcggttcgac ggcgaatacg ggctgtaatg ctgcggatcg gccgcgaaca
     2401 gcgcatgcac cgggctgatc gcgagcgccg aggcgccgtg cgccccgagg gcggcggcgt
     2461 gctcggcgag ggccgagaaa tcgccgacgc cgccgtctgc gccgcggcgc aggccgtaga
     2521 gctgggcagc gaagccgaag ccgcgctcgc gcccggcgag ccgggcaaag cccggggcgc
     2581 ccgccggggc cagcgccagc gcctgcgcgt gcccgtcgag cctcagcgtg tagtagccgg
     2641 catcgagcag cagccccggc agcgccgcgc gcccggcggc atcggcgatg agccgcgtcc
     2701 cgacgcactc gccggcctca tcgacgagtt cgaccgccgc gccgggctgc gtgccgaagg
     2761 ccgccggcag cgtccagggc tcggcgctgc gcagcaccgc gagcgccggc ggcgctgcgg
     2821 cctcggccag cagccgcgcg cggctgtcgg cgatcgcggc cgcacccgtg cagtcgatgc
     2881 cgagggcttc gagcacctgc tgctgcgtgg cgggggcgat ggcgtggcgg atgccgcggt
     2941 aatcggtcca ttcggccgcc agtccggcgg tctgggcaag tgcggcaaga tcggccgggg
     3001 cgtggggcat cttggacttc cgtcaggggg aatcggggac cgtgcctgcg gacagccaga
     3061 ccgtcgtggc agcagcggcc agatcatcac gcgccagcgc cgcggcagcg ccgtcccggc
     3121 tctcgtacag cagcgagccg gccggcggca cgaagtcgag ctcgaccggc atgtcggcga
     3181 gattcgtgta gatcgtcagc atcgtaccgt ccccgaggcg ccagcgggca cgcagcgccg
     3241 ccggtccgag cgctgcgctg ccgagcgccc gcgtgccggc cagccgcggc gcgagcaagg
     3301 tgttgcgcag gcgcagcagc atcgcgaccc gggcagcatc ctcgggatcg ccgggctgcg
     3361 ggcgcgagga ctcgaacgtc gtcccggcgt tcgggtccgg gatcgcggcg cggcgttcgg
     3421 gatcggcgaa ggccgcgaac tgcgcgaact cgcgccggcg gccctcgcgc acggcctcgg
     3481 cgagttcgtc gtggaagtcg gtgaagaaca ggaacggcgt ggtcgagccg agttcgtcgc
     3541 ccatgaacag cagcggcacc tgcgggcaga gcagctgcac ggccagcgcc gcgcgccagg
     3601 cttcgagccg gccggcctgc gcgcagagcg ccagcagccg ctcgccgagc gcgcggttgc
     3661 cgatctggtc gtggttctgc aggaagaaca cgaaggccga cggcggcagg tgcgcgctcg
     3721 gcgtgccgcg cggccggccg tcgtggcccg gcgagggctc gccctgccag ccgaagcctt
     3781 cgccgaggca gcgcacgaac agctcgccgg cacgc
//
LOCUS       DAOQJH010000202         3812 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_14862_length_3812_cov_4.268299, whole genome shotgun sequence.
ACCESSION   DAOQJH010000202 DAOQJH010000000
VERSION     DAOQJH010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3812)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3812)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3812
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14862_length_3812_cov_4.268299"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(66..476)
                     /locus_tag="PLQ18_05820"
     CDS             complement(66..476)
                     /locus_tag="PLQ18_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013538672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HNE01205.1"
                     /translation="MHQTARQLLRFGAVGIAGLVIDVAVLEAALALGAGFYGGRTLSF
                     LAAASGTWLLNRLWTFRSETARANRGLAGEWARYVALMLAGGSVNYAVYALCIETSAQ
                     ARTWPALAVAAGSLAGMVVNYASARWLVFRSGTA"
     gene            complement(487..2280)
                     /locus_tag="PLQ18_05825"
     CDS             complement(487..2280)
                     /locus_tag="PLQ18_05825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-beta-1,3-glucanase"
                     /protein_id="HNE01206.1"
                     /translation="MYDASVFRRPAFVAVALATLTAALVWWYAARPVDIGIDWHEGTW
                     NSASFAPFRAGQSPLTRNWPTPEQVENDLAHVKTAFRGVRTYAAIGAAQDVPALAARH
                     GLKVIHGAWLSSTPADNEAEVSALIEAANRYPQSVERVIVGNEVLLRRDLDAGQIIRH
                     LDRVRQSIRQPVSYADVWAFWLKNPQLADHVDYLTIHILPYWEDEPASVTDMEQRFVE
                     VIGKIKAAFPGKPILIGEAGWPTEGRSRGPAIADLPNAAHFLRRLPQLAAQYGFDYNV
                     VEAYDQLWKAQQEGTVGARWGLYDVDRQQKFGTTGPVDPFPEAGLRAGASLLGGLALA
                     LWLIPAGFGLTRALAVAGLAQLLAAGAIDTLWHAQRLAISPASVSWCLQRLLFFAADT
                     GLLDKPTVSELYRGYMDTTPALAEAWAWVRILATVVATGVLAALLRARLFGREAPGLA
                     RAARLGYLSYVAGALMISAMLAFNGRYLDIPIPEFWLPALGLPALWLAARLAGSPRGY
                     GLAALTAPGPARLKLGLWLLAGAVAVILGEGFAIVGEDFTAMHPDWSERAPLIWHAFF
                     WNRQILACALMLVWLALPLLAEWRRPAAAAT"
     gene            complement(2302..>3812)
                     /locus_tag="PLQ18_05830"
     CDS             complement(2302..>3812)
                     /locus_tag="PLQ18_05830"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNE01207.1"
                     /translation="SGFSNDAALHYLDLYRYGGAFDMIAALLVPLSPFGEYETRHLLG
                     GLLGIVGLLGAWRLGRLLGGDRAGLLALVLLLLTPCFIGHSFTNPKDAPFATAMLWAV
                     YAIVRVQHELPRPSARTLTLLGVTIGLALSVRVGAILLGPYLALGVLLWLAGRWRAGE
                     RPARLAADAARVALRCAPAFVLAYVVMGVFWPYAWQSPLNPLKTLGEFSHVAFPIETL
                     AAGEWYPVNAIPRWYVPAYLVFTLPEIVLAGLASGLAFALLRLRHRWPMSARAQSLYV
                     LGTAGVLPVLIAVALHTTAYNGLRHFLFVVPPLVVCAALALERMHEMLAARAPLHGRL
                     HTLALAALLAVQAGILGYLHPHQYIYYNVLIGGVPGAADRWELDYWGNSLPEAIDGLA
                     EYLTNEYEGAPIARRYRVAVCGKEESARYYFPDFLVFEPRWESADFLIALTNDGCDEQ
                     LRLPNAVEIDRFGVPLSVVKDLRGYVLEYNPAFKPHRSRPKFLLYLRRYGKP"
BASE COUNT          615 a         1282 c         1444 g          471 t
ORIGIN      
        1 ctgttcctgg agctgaccgg gaaggaactg cgcgaggcct gaagcgggaa ggggcgcagc
       61 ggctttcagg ccgtgccgct gcgaaacacc agccagcggg cgctggcgta gttgacgacc
      121 atgccggcca gcgaaccggc ggcgaccgcc agcgccggcc acgtgcgcgc ctgcgccgag
      181 gtttcgatgc agagcgcgta caccgcgtag ttgacgctgc cgccggccag catcagcgcc
      241 acgtagcgcg cccattcacc ggcgagtccg cggttggcac gtgcggtttc gctgcggaag
      301 gtccagagcc ggttcagcag ccaggtgccg cttgccgcgg cgaggaagga cagcgtccgg
      361 ccgccgtaga agccggcgcc gagcgcgagt gcggcttcga gcaccgccac gtcgatcacg
      421 agtccggcga tgccgacggc tccgaagcgc agcagctgcc gggcggtctg gtgcatcgcg
      481 acggcctcag gtcgcggccg cggccggccg gcgccattca gcgagcagcg gcagcgccag
      541 ccagaccagc atcagcgcgc aggcgaggat ctgccggttc cagaagaacg cgtgccagat
      601 cagcggcgcg cgctccgacc agtccgggtg catggccgtg aagtcctcgc cgacgatggc
      661 aaagccctcg ccgaggatca cggcgacggc gccggcgagc agccacaggc caagcttcag
      721 gcgcgccggc cccggtgcgg tcagggcggc cagtccgtag ccgcgcgggc tgcctgcgag
      781 gcgcgccgcc agccacagcg ccggcaggcc gagcgccggc agccagaatt ccgggatcgg
      841 gatgtcgagg tagcggccgt tgaaggccag catcgcgctg atcatcagcg caccggcgac
      901 gtagctgagg taaccgaggc gcgcggcgcg cgccaggccc ggggcctcgc ggccgaacag
      961 ccgggcgcgc agcagcgcgg ccagcacgcc ggtggcgacg acggtggcga ggatgcgcac
     1021 ccaggcccag gcttcggcga gcgccggcgt cgtgtccatg tagccgcgat acagctcgct
     1081 gacggtcggc ttgtcgagca ggccggtgtc ggcggcgaaa aacagcagcc gctgcaggca
     1141 ccagctcacc gaggccggcg agatcgccag gcgctgcgcg tgccagaggg tgtcgatcgc
     1201 accggcggcg agcagctgtg caagcccggc gacggcgagt gcgcgcgtga ggccgaagcc
     1261 ggccgggatc agccaaagcg ccagcgccag cccgccgagc agcgaggcgc cggcgcgcag
     1321 cccggcttcc gggaacggat cgaccggacc ggtggtgccg aatttctgct ggcgatcgac
     1381 gtcgtacagg ccccagcgcg cgccgaccgt gccttcctgc tgcgccttcc agagctggtc
     1441 gtaggcctcg acgacgttgt aatcgaagcc gtactgcgcg gcgagctgcg gcagccggcg
     1501 caggaagtgc gcggcgttcg gcaggtcggc gatcgccggg ccgcgcgagc ggccttcggt
     1561 cggccagccg gcctcgccga tcaggatcgg cttgcccggg aaggcggcct tgatcttgcc
     1621 gatgacctcg acgaagcgct gctccatgtc ggtgaccgag gccggctcgt cctcccagta
     1681 cggcaggatg tggatggtga ggtagtcgac atggtcggcg agctgcggat tcttcagcca
     1741 gaaggcccag acatcggcgt aggacaccgg ctggcggatc gactgccgca cgcggtcgag
     1801 gtgccggatg atctgcccgg cgtcgaggtc gcggcgcagc agcacctcgt tgccgacgat
     1861 cacgcgctcg accgactgcg gatagcggtt ggcggcctcg atcagcgcgc tgacctcggc
     1921 ctcgttgtcg gccggggtgc tgctgagcca ggccccgtgg atgaccttga ggccgtggcg
     1981 ggcggcgagc gccggcacgt cctgcgcggc gccgatggcg gcgtaggtgc gcacgccgcg
     2041 aaaggcggtc ttcacgtgcg cgagatcgtt ctcgacctgt tcgggcgtcg gccagttcct
     2101 ggtcagcggg ctctgcccgg cgcgaaacgg tgcgaacgag gcgctgttcc aggtgccttc
     2161 gtgccagtcg atgccgatgt cgaccgggcg ggcggcatac caccagacca gggcggcagt
     2221 gagcgtcgcg agcgcgacgg cgacgaacgc cggacggcgg aagacggatg cgtcgtacac
     2281 gggagtcggg acctcgacgg gctagggctt gccgtagcgg cgcaggtaga gcaggaactt
     2341 cgggcgggag cggtgcggct tgaaggccgg gttgtattcg agcacgtagc cgcgcaggtc
     2401 cttgaccacg gacagcggta cgccgaagcg gtcgatctcg acggcgttcg gcaggcgcag
     2461 ctgttcgtcg cagccgtcgt tggtcagcgc gatcaggaag tcggcgctct cccagcgcgg
     2521 ctcgaacacc aggaaatccg ggaagtaata ccgggcgctt tcttccttgc cgcagaccgc
     2581 gaccctgtag cggcgggcga tcggcgcgcc ctcgtactcg ttggtcaggt attcggcgag
     2641 gccgtcgatg gcctcgggca gggagttgcc ccagtaatcg agttcccagc gatcggccgc
     2701 gcccggcacg ccgccgatca gcacgttgta atagatgtac tggtgcgggt gcaggtagcc
     2761 gaggatgccg gcctgcacgg ccagcagcgc ggcgagcgcg agcgtgtgca ggcggccgtg
     2821 cagcggcgcg cgcgcggcga gcatctcgtg catgcgctcc agcgccagcg ccgcgcagac
     2881 caccagcggc ggcacgacga acaggaaatg gcgcaggccg ttgtaggccg tggtgtgcag
     2941 ggcgaccgcg atcagcaccg gcagcacgcc ggcggtgccg agcacgtaca gcgactgggc
     3001 gcgtgcggac atcggccagc ggtggcgcag gcgcagcagc gcgaaggcca gccccgaggc
     3061 caggccggcc agcacgatct ccggcagcgt gaacaccagg taggccggca cgtaccagcg
     3121 cgggatggcg ttgaccggat accactcgcc ggcagcgagc gtctcgatcg ggaaggcgac
     3181 gtgcgagaac tcgccgagcg tcttcagcgg gttcagcggc gactgccagg cgtagggcca
     3241 gaacacgccc atgaccacgt aggcgagcac gaacgccggg gcgcagcgca gcgccacgcg
     3301 cgcggcatcg gcggcgagtc gcgccggccg ctcgccggca cgccagcgcc cggccagcca
     3361 cagcagcacg ccgagcgcca gatacggccc gagcaggatc gcgccgacgc gcacgctgag
     3421 cgcaagcccg atggtgacgc cgagcagcgt gagcgtgcgt gccgagggtc gcggcagttc
     3481 gtgctgcacg cgcacgatcg cgtagacggc ccacagcatc gcggtcgcaa acggcgcgtc
     3541 cttggggttg gtgaagctgt ggccgatgaa gcacggcgtc agcagcagca gcacgagcgc
     3601 cagcagcccg gcacgatcgc cgccgagcag ccgcccgagc cgccaggccc cgagcaggcc
     3661 gacgatgccg agcaggccgc cgagcaggtg gcgggtctcg tattccccga acggcgacag
     3721 cggcacgagc agcgccgcga tcatgtcgaa ggcgccgccg tagcggtaca ggtcgaggta
     3781 gtgcagcgcc gcgtcgttgc tgaagccgct gc
//
LOCUS       DAOQJH010000203         3798 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_14943_length_3798_cov_4.671921, whole genome shotgun sequence.
ACCESSION   DAOQJH010000203 DAOQJH010000000
VERSION     DAOQJH010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3798
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14943_length_3798_cov_4.671921"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1850)
                     /locus_tag="PLQ18_05835"
     CDS             complement(<1..1850)
                     /locus_tag="PLQ18_05835"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HNE01208.1"
                     /translation="MNAENPLLDFSGLPRFAEIRPEHVEPAVDALLAENRAGLAQLLA
                     QPGPYTWANLIEPLEAMQDRLNRAWGPVGHLNAVMNSEALRAAYNACLPKLSAYYTEY
                     NQNEALYRATQAIRDGDEFARLDAAQRKVIDDSLRDFRLAGIALPAEKKARYGEIVQE
                     LSLLGAKFSEHVLDATQAWSLHLDSENRLAGLPASALGLARQAAVARGLDGWLLTLEF
                     PSYQPVMSYAQDRSLRESLYFAYATRAAGNFPAGAQWDNTAVVGRILELRHELARLLD
                     FPDYAQYSLATKMADDPATVLDFLRDLGARARPQAGREFEELSAFARERLGLEKLEAW
                     DVGYASEQLKQARYAISDEELRPYFPETRVVPGLFQLVEKLFGVSIRAAEGVEVWHPD
                     VRAYDIFAADGALRARFYFDLYARANKRGGAWMDSAIARRRTADGLQLPVAYMVCNFT
                     PPVGDDPALFTHAEVQTLFHEFGHGLHHMLTTVDYAAVSGISGVEWDAVELPSQFMEN
                     FCWERVTLDLIAGHYRTREPIPEALYERMIAAKNFQSAMITVRQLEFGLFDLRLHREY
                     DPARGARVLELLEEVRDEVAVVRPPAFNRYPMSFSHIFAGGYAAGYYSYK"
     gene            complement(1918..2508)
                     /locus_tag="PLQ18_05840"
     CDS             complement(1918..2508)
                     /locus_tag="PLQ18_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015279468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HNE01209.1"
                     /translation="MAAIPQFEDYTGAEPGTIRVADYLAGEDSAEVRSEYLGGLVYAM
                     TGASVRHNRIVGNTFAALRSRVPAGCEVFFESVKLHLRLQGLDWFYYPDAMVCCDPVD
                     DHPLYRERPCLLVEVLSESTARIDRREKYLSYTQIAGLRGYLLLDQDAARAQLHRCDD
                     DGLWQQQSFDDPAAVLDLPCAGAVPLAELYAGLHIG"
     gene            2660..>3798
                     /gene="gorA"
                     /locus_tag="PLQ18_05845"
     CDS             2660..>3798
                     /gene="gorA"
                     /locus_tag="PLQ18_05845"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HNE01210.1"
                     /translation="MSRYDYDLITIGAGSGGLSVAERAAAYGRRCVIIEAGPLGGTCV
                     NVGCVPKKIMWFAAHHAHALADAAGFGFDVHVGGHDWAALKRARDGYVGRIVEWYGGW
                     LADQHIELIRGRAHCIDAHTVEVEGRRLSAEHLVIATGGLPKQPDIPGASFGIDSNGF
                     FALEARPARAVVVGAGYIAVELAGVLRALGTDTTLLLRKDTVLREFDPMLGETLMSAL
                     AADGVRILTRTEPQRIERRAGGSLHIERSHGDPIDTDCLLWAIGRAPATAGLNLEAAG
                     VALAEDGTIPVDDYQNTAVPGVYALGDVTGRAALTPVAIAAGRRLADRLFGGQPDRHL
                     DYGLIPSVVFTHPPLGTVGLTEPEARSRYGDEAVRVYTSRFTPMAR"
BASE COUNT          592 a         1314 c         1350 g          542 t
ORIGIN      
        1 cacttgtagc tgtagtaacc ggccgcgtag ccgccggcga agatgtgcga gaagctcatc
       61 ggatagcggt tgaacgccgg cggacgcacg accgcgacct cgtcgcggac ctcctccagc
      121 agttcgagca cgcgggcgcc gcgcgccggg tcgtactcgc ggtgcaggcg caggtcgaac
      181 agcccgaatt cgagctggcg caccgtgatc atcgccgact ggaagttctt cgcggcgatc
      241 atgcgttcgt agagcgcctc ggggatcggc tcgcgcgtgc ggtaatggcc ggcgatcagg
      301 tcgagcgtga cgcgctccca gcagaagttc tccatgaact gcgagggcag ctcgacggca
      361 tcccactcga cgccggaaat cccggacacg gccgcatagt cgaccgtcgt cagcatgtgg
      421 tgcaggccgt ggccgaactc gtggaacagc gtctgcacct cggcgtgcgt gaacagcgcc
      481 gggtcgtcgc cgaccggcgg cgtgaagttg cagaccatgt acgcgaccgg cagctgcaga
      541 ccgtcggccg tgcgccggcg cgcgatcgcc gaatccatcc aggcgccgcc gcgcttgttg
      601 gcgcgcgcgt acaggtcgaa gtagaagcgc gcgcgcagtg cgccgtcggc ggcgaagatg
      661 tcgtaggcgc gcacgtccgg atgccagacc tcgacgcctt cggcagcgcg gatcgacacg
      721 ccgaacagct tctcgaccag ctggaacagg cccggcacga cgcgcgtctc cgggaagtac
      781 gggcgcagct cctcgtcgga gatcgcataa cgcgcctgct tgagctgctc ggaggcgtag
      841 ccgacatccc aggcttcgag cttttccagg ccgagacgct cgcgcgcgaa ggccgacagc
      901 tcctcgaact cgcgcccggc ctgcggccgg gcccgggcgc cgagatcgcg caggaagtcg
      961 agcaccgtcg ccggatcgtc ggccatcttc gtggccagcg agtactgcgc gtagtcgggg
     1021 aaatcgagca ggcgcgccag ttcgtggcgc agttcgagga tgcgcccgac gacggcggtg
     1081 ttgtcccact gcgcgccggc cgggaagttg ccggcggcgc gcgtcgcgta ggcgaagtac
     1141 agcgactcgc gcagcgagcg gtcctgcgcg tagctcatga ccggctggta ggacgggaac
     1201 tccagcgtca gcagccagcc gtcgaggccg cgcgccacgg cggcctgacg cgcgaggccg
     1261 agcgccgagg cgggcaggcc ggcgaggcga ttctcgctgt cgaggtgcag cgaccaagcc
     1321 tgggtggcat cgagcacgtg ctcggagaac ttcgcaccga gcagcgagag ttcctgcacg
     1381 atctcgccgt agcgggcctt tttctcggcc ggcagcgcga tgccggcgag ccggaagtcg
     1441 cgcagcgaat cgtcgatgac cttgcgctgc gcggcatcga gccgcgcgaa ctcgtcgccg
     1501 tcgcggatcg cctgcgtggc gcggtacagc gcctcgttct ggttgtattc ggtgtagtag
     1561 gccgagagct tcggcaggca ggcgttgtag gcggcgcgca gcgcctcgga attcatcacc
     1621 gcgttcaggt gcccgaccgg cccccaggca cggttgagcc ggtcctgcat cgcctccagc
     1681 ggttcgatca ggttcgccca ggtgtacggg ccgggctgcg cgagcagctg cgcgaggccg
     1741 gcgcggtttt cggcgagcag ggcatcgacg gcgggctcga cgtgttcggg gcgaatctcg
     1801 gcgaagcgcg gcaggccgga gaaatcgagc agcggatttt cggcgttcat ggcggtcctg
     1861 aaggggctgg ggaattcggg aacgatccgg gaacgaggca ggcgcggcgc ggccggatca
     1921 gccgatatgg aggccggcat agagttcggc aagaggcacg gcaccggcgc agggcagatc
     1981 gagcacggcc gcggggtcgt cgaaggactg ctgctgccag agaccgtcgt cgtcgcaacg
     2041 atgcagctgc gcacgcgcgg catcctgatc gagcagcaga tagccgcgca ggccggcgat
     2101 ctgggtatag gacaggtatt tctcgcgccg gtcgatgcgc gccgtcgact ccgacagcac
     2161 ctcgaccagc aggcacggac gctcgcgata cagcggatga tcgtcgaccg gatcgcagca
     2221 gaccatcgca tccgggtagt agaaccagtc gagaccttgc agtcgcaggt gcagcttcac
     2281 cgactcgaag aacacctcgc agccggccgg cacgcggctg cgcagcgcgg caaaggtgtt
     2341 gccgacgatg cggttgtgcc ggacgctcgc gccggtcatc gcgtatacca gtccgccgag
     2401 gtactcgctg cgcacctcgg cgctgtcctc gccagcgagg tagtccgcga cccggatcgt
     2461 gcccggctcc gcgcccgtgt agtcctcgaa ctgcggtatc gctgccatct gcggcctccc
     2521 gtatcccctg cctgacccgg accgacactc tacgccggcc acgcccggcc ggtgcaggca
     2581 ggcgggccgc ggccgccgcg gtcgctatgc ttcggggccg ctgccggcac atcggcagcg
     2641 cacgcgagga aaccatccga tgagccgtta cgactacgac ctgatcacga tcggcgccgg
     2701 ctccggcggc ctgtcggtcg ccgaacgcgc tgccgcttac ggccggcgct gcgtgatcat
     2761 cgaggccggg ccgctcggcg gcacctgcgt caacgtcggc tgcgtgccga agaagatcat
     2821 gtggttcgcc gcccatcacg cgcacgcgct cgccgatgcc gccggcttcg gcttcgacgt
     2881 gcacgtcggc ggtcacgact gggcggcgct caagcgcgca cgcgacggct acgtcgggcg
     2941 catcgtcgag tggtatggcg gctggctcgc ggaccagcac atcgagctga tccgcggccg
     3001 ggcgcactgc atcgatgcgc acacggtcga ggtcgagggc cggcggctca gcgccgaaca
     3061 cctggtgatc gcgaccggcg gcctgccgaa gcagcccgac atccccggcg cctcattcgg
     3121 catcgactcg aacggcttct tcgcgctcga agcccggccg gcgcgcgcag tcgtcgtcgg
     3181 ggccggttac atcgccgtcg aactcgccgg cgtgctgcgc gcgctcggca ccgacacgac
     3241 gctgctgctg cgcaaggaca cggtgctgcg cgagttcgat ccgatgctcg gcgaaacgct
     3301 gatgtcggcg ctcgcggccg acggcgtgcg gatcctgacc cgcaccgagc cgcagcgcat
     3361 cgaacggcgc gccggcggca gcctgcacat cgagcgcagc cacggcgatc cgatcgacac
     3421 cgactgcctg ctgtgggcga tcggccgggc gccggccacc gccggcctga acctcgaagc
     3481 cgccggcgtg gcactcgccg aagacggcac gatcccggtc gatgactacc agaacaccgc
     3541 cgtacccggc gtctacgcgc tcggcgatgt caccggccgc gcggcgctga ccccggtcgc
     3601 gatcgccgcc gggcgccggc tcgccgatag gctgttcggc ggccagccgg atcgccacct
     3661 cgactacggg ctgatcccga gcgtcgtgtt cacgcacccg ccgctcggca ccgtcggcct
     3721 gaccgagccc gaggcgcgca gccggtacgg cgacgaggcc gtgcgcgtct acaccagccg
     3781 cttcacgccg atggcacg
//
LOCUS       DAOQJH010000204         3788 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_15004_length_3788_cov_3.688990, whole genome shotgun sequence.
ACCESSION   DAOQJH010000204 DAOQJH010000000
VERSION     DAOQJH010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3788)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3788)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3788
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15004_length_3788_cov_3.688990"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..478)
                     /locus_tag="PLQ18_05850"
     CDS             complement(<1..478)
                     /locus_tag="PLQ18_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018954240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE01211.1"
                     /translation="MVMVPAPRPDLHGLTLVVGLGVSGLSAARALAHLGASFAVVDTR
                     SAPPGLAELDRIAPEAEVCCGDFGLIQAEALFARAARLIVSPGISVRTPAIAACAARG
                     VPVWGDIELFARLTDVPVIGITGSNGKSTVTTLLGEMATAAGVRPAVGGNLGTAALD"
     gene            complement(486..1568)
                     /gene="mraY"
                     /locus_tag="PLQ18_05855"
     CDS             complement(486..1568)
                     /gene="mraY"
                     /locus_tag="PLQ18_05855"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HNE01212.1"
                     /translation="MLVFLAELLTQLYSGFNVFSYLTLRAILGLLTALLLALLVGPVM
                     IRRLSQYQIGQQVRSDGPQTHLKKAGTPTMGGALILVAIAVSTLLWADLRNPYVWIVL
                     GVTLAFGAIGWVDDYRKLVKKNSKGLSAKEKYFWQTIAGALAALLLYLIAKTPVSTQL
                     IVPFFKHVAIDLGPWFLLLAYLTIVGSSNAVNLTDGLDGLAIMPTVMVAGGLGVIAYV
                     TGNSVFAGYLGIPYISGVGEMIVFCGAIFGAGLGFLWFNAYPAQVFMGDVGALALGAA
                     LGIVAVIVRQEIVLVIMGGVFVMETVSVILQVGSYKLTGRRIFRMAPIHHHFELKGWP
                     EPRVIVRFWIITVILVMIGLSTLKIR"
     gene            complement(1573..2946)
                     /gene="murF"
                     /locus_tag="PLQ18_05860"
     CDS             complement(1573..2946)
                     /gene="murF"
                     /locus_tag="PLQ18_05860"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HNE01213.1"
                     /translation="MKPQPLSHLATGLCAELTGHDTEVRGVSTDSRTIAPGELFVALA
                     GPNFDGHAWVATAAARGACAALVAHPQPLDLPQLLVPDTRLALGRLGALARAAFRGPV
                     IGLTGSNGKTTLKEMSAAILRRRARVLATRGNLNNDIGVPLTLLGLDEDHDCAVIEMG
                     ANHPGEIAYLTALVQPSVAILNNAGPCHLEGFGSLDGVARAKGEIFTGLGAQGVAILN
                     ADDRYFGYWRELVGARPALSFGLDAPADVSAVVLDAATQRVRLSLPQREHIEFELPLS
                     GRHNLRNACAAAAATYALGAGAEDIAAGLASLHPVAGRLQRLAGLHGATLVNDAYNAN
                     PASLAAGLETLAAEPGEHWLVLGDMGELGAEADALHAQSGERARACGFARLYALGSRS
                     RHAVRAFGAGGRHFDDIDSLTATLAADLAAGVAPTILVKGSRSMRMERVVRALDPHDH
                     DPLAGAH"
     gene            complement(2943..>3788)
                     /gene="murE"
                     /locus_tag="PLQ18_05865"
     CDS             complement(2943..>3788)
                     /gene="murE"
                     /locus_tag="PLQ18_05865"
                     /inference="COORDINATES: protein motif:HMM:TIGR01085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramyl-tripeptide synthetase"
                     /protein_id="HNE01214.1"
                     /translation="EPPGSRPPAMFLFAELDIGAAVLNLDDAFGRSLCTVARAPIHGY
                     GLDVLDTEGLAGWVQGSEPVCDTDGLRLSVHTHLGAAELASTLLGRFNAQNLLACLAA
                     LLALGMPLDEAVGRLARSRTVPGRMERFGGGADRPLAVVDYAHTPNALQSVLTALRAH
                     TAGRLYCVFGCGGDRDAGKRPLMGAAAERLADRVILTSDNPRSEDPLAILADIRAGCA
                     EPARVQVEPERAAAIRQALAAARPGDVVLIAGKGHEDYQIIGSERRVYSDRAEVAALF
                     GETSA"
BASE COUNT          585 a         1331 c         1383 g          489 t
ORIGIN      
        1 gatcgagcgc cgccgtgccg aggttgccgc cgaccgccgg gcgcacgccg gcggcggtgg
       61 ccatctcgcc gagcagcgtg gtcacggtcg acttgccgtt cgagccggtg atgccgatca
      121 ccggcacgtc cgtcaggcgc gcgaacagct cgatgtcgcc ccagaccggc acgccgcgcg
      181 ccgcgcaggc ggcgatcgcc ggcgtgcgca cgctgatgcc ggggctgacg atcagccggg
      241 cggcgcgcgc gaacagggct tcggcctgaa tcaatccgaa gtcgccgcag cagacctcgg
      301 cctcaggggc gatgcgatcg agctcggcaa gcccgggcgg tgcgctgcgc gtgtcgacga
      361 cggcgaagct cgcgccgagg tgcgcgagcg cgcgcgccgc cgacaggccg gagacgccga
      421 gcccgacgac cagcgtcagg ccgtggagat cgggacgggg agccgggacc atgaccatgg
      481 gcggctcacc ggatcttcag cgtggacagg ccgatcatca cgaggatcac cgtgatgatc
      541 cagaagcgca cgatcacgcg cggctccggc cagcccttga gttcgaagtg gtgatggatc
      601 ggcgccatcc ggaagatgcg ccggccggtc agcttgtacg agccgacctg caggatcacc
      661 gacacggtct ccatgacgaa cacgccaccc atgatcacca gcacgatctc ctgacgcacg
      721 atcacggcga cgatgccgag ggccgcgccg agcgcgagcg cgccgacatc gcccatgaac
      781 acctgcgccg gataggcgtt gaaccacagg aagccgaggc cggcgccgaa gatcgcgccg
      841 cagaacacga tcatctcgcc gacgccggag atgtacggga tgccgagata gccggcgaac
      901 accgagttgc cggtcacgta ggcgatgacg ccgaggccgc cggcgaccat gaccgtcggc
      961 atgatcgcca gcccgtcgag gccgtcggtc aggttcacgg cgttgctgga gccgacgatg
     1021 gtcaggtatg cgagcagcag gaaccacgga ccgaggtcga tcgcgacgtg cttgaagaac
     1081 ggcacgatca gctgcgtcga caccggcgtc ttggcgatca ggtacagcag cagcgcggcg
     1141 agcgcgccgg cgatggtctg ccagaagtat ttctccttcg ccgacaggcc cttgctgttc
     1201 ttcttgacca gcttgcggta gtcatcgacc cagccgatgg cgccgaaggc cagcgtcacg
     1261 ccgagcacga tccacacgta gggattgcgc aggtccgccc acagcagcgt cgagaccgcg
     1321 atcgcgacca ggatcagcgc gccgcccatc gtcggcgtgc cggccttctt caggtgcgtc
     1381 tgcggcccgt cgctgcgcac ctgctggccg atctggtatt gcgacagccg gcggatcatc
     1441 accggcccga ccagcagcgc gagcagcagc gccgtcagca gcccgaggat cgcgcgcagc
     1501 gtcaggtacg aaaagacatt gaagccgctg tacagctggg tcagcagctc ggccaggaaa
     1561 accagcattt gttcagtgcg ctcccgccag ggggtcgtgg tcatggggat cgagggcccg
     1621 cacgacgcgc tccatgcgca tcgaacgcga gcccttgacc agaatcgtcg gcgccacgcc
     1681 ggcggcgaga tccgccgcga gcgtcgccgt caggctgtcg atgtcgtcga agtgccggcc
     1741 accggcaccg aaggcccgca ccgcgtgccg gctgcggctg cccagcgcgt acaggcgcgc
     1801 gaagccgcag gcacgcgcgc gttcgccgct ctgggcgtgc agcgcgtcgg cctcggcccc
     1861 gagctcgccc atgtcgccga gcaccagcca gtgctcgccg ggctcggccg ccagcgtctc
     1921 cagcccggca gcgagcgagg ccggattggc gttgtaggcg tcgttgacga gcgtcgcgcc
     1981 gtgcaggccg gcgaggcgct gcaggcgccc ggcgaccgga tgcagcgacg caaggccggc
     2041 ggcgatgtct tcggcgcccg cgccgagcgc ataggtcgcg gcggcggccg cgcaggcgtt
     2101 gcgcaggttg tgccggccgc tcagcggcag ttcgaactcg atgtgctccc gctgcggcag
     2161 gctcaggcgc acgcgctgcg tggcggcatc gaggacgacg gcgctcacgt cggccggtgc
     2221 gtccagcccg aagctcagcg ccgggcgggc gccgaccagc tcgcgccagt agccgaagta
     2281 gcggtcatcg gcgttgagga tcgccacgcc ctgcgcaccg agcccggtga agatctcgcc
     2341 cttggcgcgc gcgacgccgt cgaggctgcc gaagccttcg aggtggcagg gaccggcgtt
     2401 gttgaggatc gcgaccgagg gctgcaccag ggcggtcagg taggcgatct cgccggggtg
     2461 gttggcaccc atctcgatga ccgcgcagtc gtgatcctca tcgaggccga gcagcgtcag
     2521 cggcacgccg atgtcgttgt tcaggttgcc gcgcgtcgcc agcacccggg cgcgccggcg
     2581 caggatcgcc gccgacatct ccttcagcgt ggtcttgccg ttggagccgg tcaggccgat
     2641 caccggaccg cggaaggccg cgcgcgccag cgcaccgagc cgcccgagcg cgagccgggt
     2701 gtccggcacc agcagctgcg gcaggtcgag cggctgcgga tgcgcgacca gcgccgcgca
     2761 ggccccgcgc gcggccgcgg tcgcgaccca ggcgtgaccg tcgaagttcg ggccggccag
     2821 cgcgacgaac agctcgccgg gtgcgatcgt gcgcgaatcg gtcgacacgc cgcggacttc
     2881 ggtgtcgtgg ccggtcagtt cggcgcacag tccggtcgcg aggtgcgaga gcggctgcgg
     2941 cttcatgcag atgtctcccc gaacagggcg gcgacctcgg cgcggtcgct gtacacgcgc
     3001 cgttcgctgc cgatgatctg gtaatcctcg tggcccttgc cggcgatcag caccacgtcc
     3061 ccggggcggg ccgccgccag cgcctggcgg atggccgcgg cgcgctcggg ctcgacctgc
     3121 acgcgcgcgg gctcggcgca gccggcccgg atgtcggcga ggatcgccag cgggtcctcg
     3181 ctgcgcggat tgtcgctggt caggatcacg cgatcggcga gacgctcggc ggccgcgccc
     3241 atcagcggac gcttgccggc atcgcgatcg ccgccgcagc cgaacacgca gtacagccgt
     3301 ccggccgtgt gcgcgcgcag cgcggtcagc accgactgca gcgcgttcgg cgtgtgggcg
     3361 tagtcgacga ccgcgagcgg gcggtccgca ccgccgccga agcgctccat gcgccccggc
     3421 accgtgcgcg agcgcgcaag ccggccgacg gcctcatcga gcggcatgcc gagcgcgagc
     3481 agcgccgcga ggcaggcgag caggttctgg gcgttgaagc gcccgagcag cgtgctcgcg
     3541 agttcggcgg ccccgaggtg ggtgtgcacg ctcaggcgca ggccgtcggt gtcgcagacc
     3601 ggctcgctgc cctgcaccca gccggccagc ccctcggtgt cgagcacatc gaggccgtag
     3661 ccgtgaatcg gcgcgcgcgc caccgtgcac agcgagcgcc cgaaggcgtc atcgaggttc
     3721 agcaccgcgg cgccgatgtc gagttcggcg aacaggaaca tggcaggtgg tcgagaccca
     3781 ggcggctc
//
LOCUS       DAOQJH010000205         3784 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_15033_length_3784_cov_4.397426, whole genome shotgun sequence.
ACCESSION   DAOQJH010000205 DAOQJH010000000
VERSION     DAOQJH010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3784
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15033_length_3784_cov_4.397426"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1189
                     /locus_tag="PLQ18_05870"
     CDS             <1..1189
                     /locus_tag="PLQ18_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNE01215.1"
                     /translation="MIFWGMGISQHVHGTDNARCLIALALATGQIGRPGTGLHPLRGQ
                     NNVQGASDAGLIPMVFPDYRRVDDPEARARFERLWGTALDPVPGLTVVEIMHAILEGR
                     IRGMYILGENPAMSDPNLHHARAALAALEHLVVQDLFLTETAAFADVVLPASAFPEKT
                     GTFTNTDRRVQLGRAALDPPGEARQDLWIIQQIARRLGLDWDYPDARAVFDEMRQAMD
                     SIAGITWERLEAEHAVTYPCLHEGDPGEPVIFTDRFPRPSGRARFVPAALIPAAERPD
                     ADYPCVLITGRQLEHWHTGSMTRRAGVLDALEPEAIVTLHPEDYAALNPGPDGLRVRL
                     ETRRGQLSARARPDPALMRGQVFMAFCYVEAAANLLTVDALDPYGKIPEFKYCAVRVV
                     RAG"
     gene            complement(1251..1748)
                     /locus_tag="PLQ18_05875"
     CDS             complement(1251..1748)
                     /locus_tag="PLQ18_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNE01216.1"
                     /translation="MPAPPEALQAHHACTAFRCGRPALDDWLQRHALQAQGSGSARSF
                     VVCEAGRVIAYYSLTVGQIEPAAAPDRIRQGMGRFPLPVVLLARLAVDGAYQGRGIGA
                     ALLRDAIRRSLIIAEQAGVRAMLVHPLDEHAARFYDACGFVPAPFGAGHRLLLLKDAR
                     RLMAQ"
     gene            complement(1748..2038)
                     /locus_tag="PLQ18_05880"
     CDS             complement(1748..2038)
                     /locus_tag="PLQ18_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HNE01217.1"
                     /translation="MSESPARSPRSARLGLRATPEQEALLKRAAEIRHKSLTDFVLDS
                     ACLAAEQTLLDQRLFRISEGEYAALLELLDRPAADNPGLQQLFSKPDPWQAG"
     gene            complement(2116..2820)
                     /locus_tag="PLQ18_05885"
     CDS             complement(2116..2820)
                     /locus_tag="PLQ18_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017774375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE01218.1"
                     /translation="MQPPKPVLLVVEDELAIRRFLRTALRAQGYTLFEAATGIEALTL
                     AAQERPDLILLDLGLPDLDGLDVLGRLREWSTVPVIILSARGQEADKVRGLDAGADDY
                     LAKPFGVAELLARLRVALRHRSRSGTADPVFTAGGLSVDLAAHRVTRDGSDIHLTPRQ
                     FRVLAVLVRHAGRVVTQQQLIREVWGEASDATSDSVRIAMHGLRHRLEPDPVRPRWLR
                     TEPGIGYRLVGEEIAD"
     gene            complement(2820..>3784)
                     /locus_tag="PLQ18_05890"
     CDS             complement(2820..>3784)
                     /locus_tag="PLQ18_05890"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNE01219.1"
                     /translation="WVHDLGRAAGLGTATLPYVDAVYVPLLGKRGAVGALRVKPALAE
                     RLVLPEQMRLLEAFANQTALALEVDRLGEEAREAKLAAETERLRSSLLASVSHDLRTP
                     LAAIIGSASSLAEYGDGLDAEERRELALNIHAEAERLNRLVANLLQATRLEAGTLTPL
                     REAHDLEAVIGSALERLSRALAGRPFHTDIADGLPLVSIDALLIEQVFVNLIDNALRY
                     TPAGSPIELTVRLEDGDVRVELADRGPGLPAAALGQVFERFWRGAPDSHPGGAGLGLA
                     ICRGIVELHGGRIEALNRTGGGAVFRFTLPVALPLALADAGELP"
BASE COUNT          566 a         1380 c         1317 g          521 t
ORIGIN      
        1 gatgatcttc tggggcatgg gcatcagcca gcacgtgcac ggcaccgaca acgcccgctg
       61 cctgatcgcg ctcgcgctcg cgaccggcca gatcggccgg cccggcaccg gcctgcatcc
      121 gctgcgcggc cagaacaacg tgcagggggc ctcggatgct ggccttattc cgatggtgtt
      181 cccggactac cggcgcgtcg atgaccccga agcccgcgcg cgcttcgagc ggctgtgggg
      241 cacggcgctc gatccggtgc cgggcctgac cgtcgtcgag atcatgcacg cgatcctcga
      301 aggccggatt cgcggcatgt acatcctcgg cgagaacccg gcgatgtcgg acccgaacct
      361 gcaccacgcc cgcgccgcgc tggccgcgct cgaacatctg gtggtgcagg acctgttcct
      421 gaccgagacc gcggcgtttg ccgatgtcgt gctgccggcg agcgccttcc cggagaagac
      481 cggcaccttc acgaacaccg accggcgcgt gcagctcggc cgcgccgcgc tcgacccgcc
      541 cggcgaggcc cgccaggacc tctggatcat ccagcagatc gcccgccggc tcggcctgga
      601 ctgggattac ccggacgcgc gcgcggtgtt cgacgagatg cgccaggcga tggacagcat
      661 cgccggcatc acctgggagc gcctcgaagc cgaacacgcg gtcacctacc cgtgcctgca
      721 cgaaggcgat ccgggcgagc cggtgatctt cacggaccgt tttccgcgcc cgtccggccg
      781 tgcgcgcttc gtgccggcgg cgctgattcc ggccgccgaa cgcccggatg ccgactatcc
      841 gtgcgtgctg atcaccggtc gccagctcga acactggcat accggcagca tgacgcgccg
      901 cgccggcgtg ctcgatgcac tcgaacccga agccatcgtg acgctgcacc ccgaggacta
      961 cgccgcgctg aaccccggcc ccgacggcct gcgggtgcgc ctcgaaaccc gccgcggcca
     1021 gctcagcgcc cgcgcccgcc ccgacccggc gctgatgcgc ggccaggtgt tcatggcctt
     1081 ctgctacgtc gaggccgccg ccaacctgct gaccgtcgac gcgctcgacc cgtacgggaa
     1141 gattccggag ttcaagtact gcgcggtgcg ggtcgtgcgc gcgggctgag ggcggacggc
     1201 agctgttaaa cagaatcccg ggctgtataa caatcacgca gcagatgtgg tcattgcgcc
     1261 atgagccgcc tggcatcctt cagcagcaac agccggtggc ccgctccgaa tggtgccggc
     1321 acgaaaccgc aagcgtcgta gaaccgcgca gcgtgctcat cgagcggatg caccagcatc
     1381 gcccgcactc cggcctgctc ggcaatgatc agtgaacggc ggatcgcatc gcgcagcagg
     1441 gcggcgccga tgccacgccc ctgatacgca ccatcgaccg ccagtcgcgc cagcagcacg
     1501 accggcagcg gaaagcggcc catgccctga cggatgcgat ccggtgcagc ggccggctcg
     1561 atctggccaa ccgtcaggct gtaataggca atcacccgtc cggcctcaca gacgacgaag
     1621 cttcgcgccg acccgctgcc ctgcgcctgc agcgcatgcc gctgcagcca gtcatcgagc
     1681 gcgggccggc cgcagcggaa tgctgtgcat gcgtgatggg cctgcagggc ctcgggtggc
     1741 gccggcatca gcccgcctgc cagggatcgg gcttcgagaa cagttgctgc aggccgggat
     1801 tgtccgcggc cgggcggtca agcagttcga gcagcgcggc gtattccccc tcgctgatcc
     1861 ggaacagccg ctgatccagc aaggtctgct cggccgccag gcaggcgctg tcgagcacga
     1921 aatcggtcag tgacttgtgc cggatttcgg cggcacgctt cagcagggct tcctgctcgg
     1981 gggtggcacg cagacccagc cgggcgctgc ggggcgaacg tgcaggagat tcggacatgg
     2041 tggcgctccg gctacgggaa tgcgcagacg ttagcacgac aggcgtacga cgatccttgt
     2101 gctgccctgc ctgcttcagt cggcgatttc ctccccgacc agccggtatc cgatccccgg
     2161 ctccgtcctg agccagcgcg gccgcaccgg gtccggctcc aggcggtgtc gcagcccgtg
     2221 catcgcgatg cgcacggaat cggaagtggc gtctgaggcc tcgccccaga cctcgcggat
     2281 cagctgctgc tgcgtgacca cgcgcccggc gtggcgcacg agcacggcga gcacgcggaa
     2341 ctgccgcggc gtcaggtgga tgtcgctgcc gtcgcgcgtg acgcgatgcg cggcgagatc
     2401 gacgctcagg ccgccggccg tgaacaccgg atcggccgtg ccgctgcggc tgcggtggcg
     2461 cagcgccacg cgcagccgcg ccagcagctc ggccacgccg aagggcttgg cgaggtaatc
     2521 gtcggcgccg gcgtcgaggc cgcgcacctt gtcggcctcc tgaccgcgcg ccgacaggat
     2581 gatgaccggc accgtgctcc attcgcgcag gcgcccgagc acgtcgaggc cgtcgaggtc
     2641 gggcaggccg agatcgagca ggatcaggtc ggggcgctcc tgggcggcga gcgtcagggc
     2701 ctcgatgccg gtcgcggcct cgaacagcgt gtagccctgc gcgcgcagcg ccgtgcgcag
     2761 gaagcggcgg atcgccagtt cgtcctcgac gacgagcagc accggcttcg gcggctggat
     2821 cacggcagtt ccccggcatc ggccagcgcg agcggcaagg ccaccggcag cgtgaagcgg
     2881 aacaccgcac cgccgccggt ccggttcagc gcctcgatgc ggccgccgtg cagctcgacg
     2941 atgccgcgac agatcgcgag tcccagcccg gcgccgcccg ggtggctgtc cggcgcgccg
     3001 cgccagaagc gctcgaacac ctgtccgagc gcggccgccg gcaggcccgg gccgcggtcg
     3061 gcgagttcga cccgcacgtc gccatcctcc agccgcaccg tcagctcgat cggactgccg
     3121 gccggcgtgt agcgcagcgc gttgtcgatc aggttcacga acacctgctc gatcagcagc
     3181 gcgtcgatcg acaccagcgg caggccatcg gcgatgtcgg tgtggaacgg ccgcccggcc
     3241 agcgcccgcg acaggcgctc cagcgcactg ccgatcacgg cttcgagatc atgggcctcg
     3301 cgcagcggcg tcagcgtgcc ggcctccagg cgcgtggcct gcagcaggtt ggcgaccagc
     3361 cggttcaggc gctcggcctc ggcgtggatg ttcagcgcga gctcccggcg ctcttcggcg
     3421 tcgagcccgt cgccgtattc ggcgaggctc gatgccgagc cgatgatcgc cgcgagcggc
     3481 gtgcgcaggt cgtgcgacac cgacgccagc agcgagctgc gcaggcgctc ggtctcggcc
     3541 gcgagcttcg cctcgcgcgc ctcctcgccg agccggtcga cctcgagcgc gagcgcggtc
     3601 tggttcgcga aggcttcgag caggcgcatc tgctcgggca gcaccaggcg ttcggcgagc
     3661 gccggcttca cgcgcagcgc cccgaccgcg ccgcgcttgc cgagcagcgg cacgtagacg
     3721 gcatcgacgt aaggcagcgt cgcggtgccg agcccggcgg cgcgaccgag gtcgtgcacc
     3781 cact
//
LOCUS       DAOQJH010000206         3783 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_15038_length_3783_cov_5.099249, whole genome shotgun sequence.
ACCESSION   DAOQJH010000206 DAOQJH010000000
VERSION     DAOQJH010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3783)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3783)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3783
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15038_length_3783_cov_5.099249"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="PLQ18_05895"
     CDS             <1..213
                     /locus_tag="PLQ18_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008290773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HNE01220.1"
                     /translation="SLLAGRPGKGQPDVGRIVCACFRVGVNTLLSAIRERKLTTPEAI
                     GAALRAGTNCGSCVPEIRKLIADNAS"
     gene            359..805
                     /locus_tag="PLQ18_05900"
     CDS             359..805
                     /locus_tag="PLQ18_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HNE01221.1"
                     /translation="MHKRITRAAFAAALALAVQIAGAAEFGTADEAKAMLEKAVAALK
                     ADKAAALSQFAKGEGGFKDRDLYPFCGGPDGNFTAHPTLTGKSLRELKDKSGKPLGEE
                     IYSTAQEGRISEVNYMWPRTGGTDPVQKVSYVTRVGDQVCAVGYYK"
     gene            complement(964..1938)
                     /gene="cysB"
                     /locus_tag="PLQ18_05905"
     CDS             complement(964..1938)
                     /gene="cysB"
                     /locus_tag="PLQ18_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012313669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HNE01222.1"
                     /translation="MKLQQLRYLCAIAKNQLNISAAAESLFTSQPGVSKQVRLLEDEL
                     GVELFNRNGKHLTHITPVGRHIIARAEAILREVRNIQILAEEFRDDARGTLSIATTHT
                     QARYALPPIIKTFRARYPEVVLQLHQGSPTQIAEMAASGAVDFAIATEAIEEFHDLVM
                     MPCYLWNRCALVPQGHPLTGSTPLTIEALARHPLVTYVFGFTGRSQLDEAFKAARLHP
                     EVAFTATDADVIKTYVRIGLGVGIIARMAWDAEADRDLVALDASHLFAPNTTKIGFRR
                     GTFLRQFQYDFIQLFAPHLTAQVVQTAEASESQEALDELFASLELPEY"
     gene            2562..3509
                     /locus_tag="PLQ18_05910"
     CDS             2562..3509
                     /locus_tag="PLQ18_05910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE01223.1"
                     /translation="MSDHPPVDAVLRIELLERFRQLHESLDALATAIGADQDRAAWLD
                     EAPVTEIRTRAATLIRDIWYGDEVEDARMTVTSIGLIGCCAETLTLAQAANAAKDAFK
                     TVVLQFRGKTSERNRALAEMLETWHARDPDLARALSAGGIGRVHLVQAYRHVPVLDET
                     PVRVGFSYASSARAIRALTRDQALELVEALPDTHAARAVYRQMLSQYPDSERFAQMRN
                     LAPNLVANLVFRDGYGEEERLRRGVMVRVRTEQVRRKLLHVHLPILFPLAAGAPLPAH
                     PARGPQVRSAASRLLRSDRRIDGPPLIPALRLYRYREPD"
     gene            complement(3551..>3783)
                     /locus_tag="PLQ18_05915"
     CDS             complement(3551..>3783)
                     /locus_tag="PLQ18_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015279389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HNE01224.1"
                     /translation="PVAAGMPPSPVPAVGATGTLVTLDEAMRSHIARALEMTHGRLEG
                     RHGAALLLGINPHTLRARMRKLGLDPAQFRPH"
BASE COUNT          706 a         1170 c         1201 g          706 t
ORIGIN      
        1 agcctgctcg ccggccgtcc cggcaagggc cagcccgatg tcggacgcat cgtctgcgcc
       61 tgcttccgcg tcggcgtcaa cacgctgctg tcggcgatcc gcgagcgcaa gctgacgaca
      121 ccggaagcga tcggtgccgc gctgcgtgcc ggcaccaact gtggttcctg cgtgcccgag
      181 atccgcaagc tgatcgccga caacgcctcc tgagcagccc ggcccgggat ttcccatgct
      241 tgcctcatgg gaaatgacgc tgctatatct cctctgcctt cacgaacgat gagggtggcc
      301 ggccgatgcc ttttccggca ccggccgacg aacataagtc aggcagagga gagacttcat
      361 gcacaagcgc atcacgcgcg cagcattcgc cgcggccctc gcgctcgccg tccagatcgc
      421 cggggccgcc gagttcggca ccgccgacga ggcgaaggcg atgctcgaaa aggccgtggc
      481 cgccctgaag gccgacaagg ccgccgccct gtcgcagttc gccaaagggg agggcggctt
      541 caaggatcgt gacctgtatc cgttctgcgg cggtccggac ggcaacttca ccgcacatcc
      601 gaccttgacc ggcaagagcc tgcgcgagct gaaggacaag tccggcaagc cgctcggcga
      661 ggaaatctac tcgacggccc aggaaggccg gatcagcgaa gtcaactaca tgtggccgcg
      721 taccggcggc accgatcccg tgcagaaggt cagttacgtg accagggtcg gcgatcaggt
      781 ctgcgcggtc ggatattaca agtgatgcct tgggcatgag ccgcagtctg caatgtgcag
      841 acgggcggac gaaacccgac aaggcgccaa acggcgcctt gtttatttat aatcaaataa
      901 ttttttatac catcatattt tacactgatc acaaatccga ttttgttcct tccaccacac
      961 ccatcaatat tccggcaact ccagagaagc aaacagctca tccagcgcct cctgggattc
     1021 ggaagcttca gcggtctgca cgacctgcgc cgtcagatgc ggtgcaaaca gctggatgaa
     1081 gtcgtactgg aactggcgca ggaaggtgcc gcgccggaaa ccgatcttgg tcgtgttggg
     1141 cgcaaacaga tggctggcat cgagcgcgac cagatcccgg tcagcttcgg catcccaggc
     1201 catgcgcgca atgatgccga cgccaaggcc gatgcggaca tacgtcttga tcacatcggc
     1261 atcggtggcc gtgaacgcga cttccggatg caggcgcgcg gccttgaacg cctcgtcgag
     1321 ctgcgagcgg ccggtgaatc cgaacacgta ggtcaccagc ggatgccggg cgagggcctc
     1381 gatcgtcaat ggcgtcgatc cggtcagcgg atggccttgc ggcaccagcg cacagcggtt
     1441 ccagaggtag cagggcatca tcaccagatc atggaattcc tcgatcgcct cggtcgcaat
     1501 cgcaaaatcg acggctccgg aggcggccat ctcggcaatc tgggtcggcg agccctgatg
     1561 cagctgcagc acgacttccg ggtaacgggc ccggaaggtc ttgatgatcg gcggcagcgc
     1621 ataacgggcc tgggtatgcg tggtcgcgat cgacagtgtg ccacgggcat cgtcgcggaa
     1681 ttcctcggcg aggatctgga tgttgcggac ttcgcgcagg atcgcctcgg cgcgggcgat
     1741 gatgtgccgg ccgaccgggg tgatgtgggt caggtgcttg ccgttgcggt tgaacagctc
     1801 gaccccgagc tcgtcctcga gcagtcgcac ctgcttggat accccgggct gcgacgtgaa
     1861 cagactctcg gctgcggcag agatgttgag ctgattcttg gcgattgcgc acaggtagcg
     1921 cagctgctgc agtttcactg acccgacctc atcggcatcg catattccgg agaagcataa
     1981 cgcagagcgg atgagttcgc gttccggctg gatgcgccac ctgctggagg aaggcagcca
     2041 cttcggcgct tgacgacggg cgttggcggt ttgcgttcat gggtgtcggg acacaggcat
     2101 caccagcccg gaagacggtc ggttcaaggc ttcagggagc catgacacgg actgtatctt
     2161 gagcctaata atgtatatta tgtcaaatac gctagagatg tcatgaaacc cgattttcct
     2221 cgatgccgac gaccgggatg ctgacgaccg gatcatgttc ccctttccac caggagcacc
     2281 cagctcacct tccggttgtt ttcgagccgc ctcctgcttt ttcgaacgcc ttcaacccct
     2341 gtgcatccga tcgccccttg cactgctcgg tccgatgatc ttccagaggc tggaatccat
     2401 gtggatgcga acgtcgatgc aatggctgga tctgcgtcgg atttcttttg ctgacgttgt
     2461 atgtgtcttg tctggaattt gggaggattt ctgaatttcg ggtgattaaa aatatgatga
     2521 tataatttca caccatcaag ctcctgccgc agggtgtgcc catgtctgac catccgcccg
     2581 tcgatgcggt cctgcgtatc gaactgctgg agcgattccg gcaactgcat gaatctctgg
     2641 atgctctggc aactgccatc ggagctgacc aggatcgggc agcgtggctg gatgaggcac
     2701 cggtcaccga aatccgcacg cgcgcagcga cgctgatccg ggatatctgg tatggcgatg
     2761 aagtcgagga tgctcgcatg acggtgacct cgatcgggct gatcggctgc tgcgccgaaa
     2821 cgctgacgct cgcgcaggct gcaaatgcag ccaaggatgc cttcaagacc gtcgtgctgc
     2881 agtttcgcgg caagaccagc gaacgtaacc gggcactggc cgaaatgctc gaaacctggc
     2941 atgcccgcga tcccgatctg gcccgtgccc tcagcgccgg cggcatcggt cgcgtgcatc
     3001 tggtccaggc gtaccggcat gtgccggtgc tcgatgaaac accggtgcgg gtcggttttt
     3061 cctatgcgag cagtgcgcgg gcaatccggg cgctgacgcg cgatcaggca ctggaactgg
     3121 tcgaggcctt gccggacaca cacgccgccc gcgccgtgta ccggcagatg ctcagccagt
     3181 atccggacag cgagcgtttt gcgcagatgc gcaacctggc accgaatctg gtcgccaatc
     3241 tcgtgttccg ggacggctat ggcgaggaag aacgcctgcg acgcggcgta atggtgcggg
     3301 tccggaccga gcaggtacgc cgcaagctgc tgcacgtgca tctgccgatc ctgtttccgc
     3361 tcgcagcagg cgctcccctg cccgctcacc cggcccgcgg cccgcaggtt cgcagcgctg
     3421 ccagccgcct gctgcgctcg gaccggcgca tcgacggtcc gccgctgatc ccggcgctgc
     3481 ggctgtaccg gtatcgcgag ccggactgag caccggtcgt gtcgatcagt cgtgccagac
     3541 catgccggcg tcagtgcgga cggaactgcg ccgggtcgag acccagcttg cgcatgcggg
     3601 cgcgaagcgt gtgcggattg atgccgagca gcagtgccgc gccatggcga ccttcaaggc
     3661 ggccatgcgt catttccagc gcgcgggcga tgtgggagcg catcgcctcg tcgagtgtca
     3721 ccagtgtccc tgtcgcaccg accgccggga cgggtgacgg aggcatgccg gcagcgaccg
     3781 gct
//
LOCUS       DAOQJH010000207         3781 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_15051_length_3781_cov_4.611916, whole genome shotgun sequence.
ACCESSION   DAOQJH010000207 DAOQJH010000000
VERSION     DAOQJH010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3781)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3781)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3781
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15051_length_3781_cov_4.611916"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..828
                     /locus_tag="PLQ18_05920"
     CDS             <1..828
                     /locus_tag="PLQ18_05920"
                     /inference="COORDINATES: protein motif:HMM:NF018168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod C-terminal
                     domain-containing protein"
                     /protein_id="HNE01225.1"
                     /translation="GNYSLLRLSDSKVIATGSGPGLSGDGLDIDITSGTPANGDRFLI
                     QPARLAASQIEVALIGTDQIAAAAPIATAAARANIGTGSISAGTVNTLPTTASDVLKP
                     VEIRFTSETTYDLVDVSDPNNPVTLQSASAYSASAGATIGVNGWEVRVSGQPQNGDVF
                     TVGPNTAGSGDNRNMLALAGLATKRVLIGSTADLKGFYAGVIGDIGTQSQRISTAVTA
                     QTALLNQAKQARESASGVNLDEEAADLMRFQQAYAASAQVMKTADELFQALLGIARG"
     gene            1022..2713
                     /locus_tag="PLQ18_05925"
     CDS             1022..2713
                     /locus_tag="PLQ18_05925"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HNE01226.1"
                     /translation="MYQYDSHDQRLVDERVAQFRGQVRRYLAGQLSDDEFRPLRLMNG
                     LYLQTHAYMLRIAVPYGLLSSKQMRMLAHIARTWDRGYGHFTTRQNIQFNWPKLEDVP
                     EILAALASVQMHAIQTSGNCIRNVTTDHLAGVAEDELEDPRLWCEIIRQWSTLHPEFS
                     YLPRKFKIAVSATAADRAATQVHDIGLYLTRNDAGETGFQVLVGGGLGRTPIIGQVIR
                     EFVAPRDLLSYLEAILRVYNLEGRRDNLTKARIKILVKALGIDEFRRRVDAEWAEIRE
                     SGLVLDEAEIERVRAFFTPPAYEASAAEDASWTGLRDADPAFARWLKRNTAGHKVAGY
                     RNVFVALKPFGQAPGDITAEQMDAVADLADRYSFGQLRSTHTQNLILADVRNADLHAL
                     WQALDALGLATPNIGTIADIICCPGLDFCSLANAGSIGVAKSIQSRFEDLDYLYDLGD
                     LRLNISGCMNSCAHQHVGAIGVLGVDKGGEEWYQITIGGSSGRDASLGDVIGPSVAKD
                     AVADTVEKLVAVYVEQREPEEPFLATVRRIGVKPFKERVYGAKSASARKPETVNG"
     gene            2706..3197
                     /locus_tag="PLQ18_05930"
     CDS             2706..3197
                     /locus_tag="PLQ18_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013338448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF934 domain-containing protein"
                     /protein_id="HNE01227.1"
                     /translation="MADLLKNRARVTDPFVSVADDAPLPAAGTPVLVTLARWQAERDA
                     LLARGDAIGVQVPGTTTAAELAADLGHWALVAIEFPVFKDGRGYTLARLLRERFAYPG
                     ELRAVGDVLRDQLFFLSRAGFDAFALRADQDFDEALTAFDDFTVRYQPAADEPLPLYR
                     RRG"
     gene            complement(3282..>3781)
                     /locus_tag="PLQ18_05935"
     CDS             complement(3282..>3781)
                     /locus_tag="PLQ18_05935"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HNE01228.1"
                     /translation="VTVATALIVGGFIILLIEARKRPATISSTDQISGRTALMIGIAQ
                     CFALIPGTSRSGATIMGALLCGLSRPAAAEFSFFLSIPTMIAATLYSLYKARDVLSST
                     DIPTFGVGFVAAFVSGFIVVKLFLAFVSKHSFNAFAWYRIALGSAVIAYFYFTGQPMF
                     GVLTD"
BASE COUNT          658 a         1322 c         1239 g          562 t
ORIGIN      
        1 ggcaactatt cgctgctgcg cctcagcgac agcaaggtga tcgcaaccgg cagcggtccg
       61 ggcctctccg gcgacggtct cgacatcgac atcacctccg gcacgccggc caacggcgac
      121 cgcttcctga tccagccggc ccggctcgcc gcttcccaga tcgaggtcgc cctgatcggc
      181 accgaccaga tcgccgctgc cgcgccgatc gcgaccgccg cggcgcgcgc caacatcggc
      241 accggcagca tcagcgccgg caccgtgaac accttgccga cgaccgcgag cgatgtgctg
      301 aaaccggtcg agatccgctt caccagcgag accacgtacg acctcgtcga tgtcagcgat
      361 ccgaacaatc cggtgacgct gcagagcgcc agcgcgtaca gcgcgagtgc cggcgccacg
      421 atcggcgtca acggctggga ggtgcgcgtc tccggccagc cgcagaacgg tgatgtgttc
      481 acggtcggtc cgaacacggc cggctccggc gacaaccgca acatgctggc gctggccggg
      541 ctggccacga agcgcgtgct gatcggcagc acggccgacc tcaagggctt ctacgccggc
      601 gtcatcggcg acatcggcac gcagtcgcag cgcatcagta ccgcggtgac ggcgcagacg
      661 gcgctgctga atcaggcgaa gcaggcgcgt gaatcggctt ccggcgtcaa tctcgacgag
      721 gaagccgccg acctgatgcg cttccagcag gcctacgcgg catcggcgca ggtcatgaag
      781 accgccgacg agctgttcca ggccctgctc ggcatcgccc gcggctgatg cgcggcccgg
      841 gtggccggca gcgcgcggcg gatgccggag gcgacgggcg ggatattccg gttacttatt
      901 tcaatcagga atttaagtat caaaaaataa tgcttcaaga atatatggga cgctgttacc
      961 gtcgcgcgcc tgatcgccgg acaagccgtc cggcgctcgc tttcgacagg tgatcgcccc
     1021 catgtaccag tacgattccc acgaccagcg gctcgttgac gagcgtgtcg cgcagtttcg
     1081 cgggcaggtc cggcgctatc tcgccggcca gctcagcgac gacgagttcc ggccgctgcg
     1141 tctgatgaac ggcctgtacc tgcagacgca cgcctatatg ctgcgcatcg cggtgccgta
     1201 cggtctgctg tcgtcgaagc agatgcgcat gctcgcgcac atcgcccgca cctgggatcg
     1261 cggctacggt cacttcacga cccgccagaa catccagttc aactggccga agctcgaaga
     1321 tgtgccggag atcctcgccg cgctcgcctc ggtgcagatg cacgcgatcc agaccagcgg
     1381 caactgcatc cgcaacgtca ccaccgacca cctcgccggt gtcgccgagg acgaactcga
     1441 agacccgcgg ctctggtgcg agatcatccg ccagtggtcg acgctgcacc cggaattctc
     1501 ctacctgccg cgcaagttca agatcgcggt cagcgccacg gccgccgatc gcgccgcgac
     1561 ccaggtccat gacatcggcc tgtacctgac gaggaacgac gccggcgaaa ccggcttcca
     1621 ggtgctggtc ggcggcggcc tcgggcgcac gccgatcatc ggccaggtga tccgcgagtt
     1681 cgtcgcgccg cgcgacctgc tgagctacct cgaagccatc ctgcgcgtct acaacctcga
     1741 aggccggcgc gacaacctga ccaaggcgcg catcaagatc ctcgtgaagg cgctcggcat
     1801 cgacgagttc cgcaggcgcg tcgacgccga gtgggccgag atccgcgaat ccgggctggt
     1861 gctcgacgag gccgagatcg agcgcgtgcg cgcgttcttc acgccgccgg cgtatgaggc
     1921 cagtgctgcg gaagatgcgt cctggaccgg gctgcgcgat gccgatccgg ccttcgcgcg
     1981 ctggctgaag cgcaacaccg ccggccacaa ggtcgccggc taccgcaacg tgttcgtcgc
     2041 gctgaagccc ttcggtcagg cgcccggcga catcacggcc gagcagatgg acgcggttgc
     2101 cgatctcgcc gaccgctaca gcttcggcca gttgcgctcg acgcacacgc agaacctgat
     2161 ccttgccgac gtgcgcaacg ccgacctgca cgcgctgtgg caggccctcg atgcgctcgg
     2221 gctggcgacg ccgaacatcg gcacgatcgc cgacatcatc tgctgcccgg gcctcgattt
     2281 ctgcagcctc gccaatgccg gttcgatcgg tgtggccaag agcatccaga gccgcttcga
     2341 ggacctcgat tacctctacg acctcggcga cctgcgcctg aacatctccg gctgcatgaa
     2401 ctcctgcgcg caccagcacg tcggcgccat cggcgtgctc ggcgtcgaca agggcgggga
     2461 ggagtggtac cagatcacga tcggcggcag ctccgggcgc gatgcctcgc tcggcgacgt
     2521 gatcggcccg tcggtcgcga aggacgcggt cgccgatacg gtcgaaaaac tcgtcgccgt
     2581 ctacgtcgag cagcgcgagc cggaagaacc cttcctcgcc accgtgcgtc gcatcggcgt
     2641 caaacccttc aaggagcgcg tctacggcgc gaagtccgct tctgcccgca agccggagac
     2701 cgtcaatggc tgacctcctc aagaaccgcg cgcgcgtcac cgacccgttc gtgagcgtcg
     2761 ccgacgacgc cccgctgccg gccgccggca cgccggtgct ggtgacgctg gcccgctggc
     2821 aggccgagcg cgatgcgctg ctcgcccgtg gcgatgccat cggcgtgcag gtgccgggca
     2881 cgacgacggc ggcggagctt gccgccgacc tcggacactg ggcgctggtc gcgatcgagt
     2941 tcccggtgtt caaggacggc cgcggctaca cgctggcgcg gctgctgcgc gagcgtttcg
     3001 cctatccggg cgagctgcgt gcggtcggcg acgtgctgcg cgaccagctg ttcttcctgt
     3061 cacgcgccgg cttcgacgcc ttcgcgctgc gcgccgatca ggacttcgac gaagccctga
     3121 ccgccttcga cgacttcacg gtccgctacc agccggccgc cgacgaaccg ctgccgctgt
     3181 accggcgccg cggctgaaac gccgcagcgg tgcagacaaa aaagcccggt gtgaaccggg
     3241 cttttttcat gagccgcaag gcagatgcgc caagacccgg ctcagtcggt cagcaccccg
     3301 aacatcggct gcccggtgaa gtaaaagtac gcgatcaccg ccgagccgag cgcgatccgg
     3361 taccacgcga aggcgttgaa gctgtgcttc gacacgaagg cgaggaacag cttcacgacg
     3421 atgaagccgg agacgaaggc ggcgacgaag ccgacgccga aggtcgggat gtcggtgctc
     3481 gaaagcacgt cgcgcgcctt gtacagcgag tacagcgtcg cggcgatcat ggtcgggatc
     3541 gacaggaaga acgagaactc ggctgccgcc ggccgcgaca gtccgcagag cagcgcgccc
     3601 atgatcgtcg cgcccgagcg cgaggtgccg gggatcagcg cgaagcactg ggcgatgccg
     3661 atcatcagcg ccgtgcggcc gctgatctgg tcggtcgagc tgatcgtcgc cgggcgcttg
     3721 cgcgcctcga tcagcaggat gatgaagccg ccgacgatca gcgccgtggc gaccgtgacc
     3781 g
//
LOCUS       DAOQJH010000208         3778 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_15074_length_3778_cov_3.787268, whole genome shotgun sequence.
ACCESSION   DAOQJH010000208 DAOQJH010000000
VERSION     DAOQJH010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3778)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3778)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3778
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15074_length_3778_cov_3.787268"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..523)
                     /locus_tag="PLQ18_05940"
     CDS             complement(<1..523)
                     /locus_tag="PLQ18_05940"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNE01229.1"
                     /translation="MRTLAHARSWYEDALPPEPPAPALAGDVRADVAVLGAGLTGLNA
                     ALELAARGLRVVLVEAARAGAGGSGRSGGQVLGDYACGMETLRGLLGREAAAQLWQWS
                     LEAVARVKNHIARHAIACDWRDGCATLALKPRQVRALRDWQAQAAHDYGYDGLRWLAA
                     GELGELVASPRYCG"
     gene            676..2013
                     /locus_tag="PLQ18_05945"
     CDS             676..2013
                     /locus_tag="PLQ18_05945"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012977188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HNE01230.1"
                     /translation="MSSIKSWFKDHQIVEVEAIIPDMSGVARGKILPAEKYYQDKGLR
                     LPEGIFLQTITGDAPDDYYDVISAADGDIVLRPDLDTICLVPWATEPTAQIIHDAYYG
                     DGRVVDIAPRHVLQRVLRLYAERGWKPVVAPELEFFLVKPNTDSDYPLEPPIGRSGRR
                     ETGRQSYGIDAVNEFEPLFEDVYDYCEKQGLGIDTLIHESGAAQLEINLLHGDALALA
                     DQAFRFKRTMREAAMRHQIYATFMAKPMQHEPGSAMHIHQSIVGLDDGRNVFSDEDGN
                     PTPLFYAHIAGLQKYLPSAMALIAPNVNSYRRINRTDVAPINVQWGYDNRTAAFRVPQ
                     ASPQSRRIENRVPGADANPYLAMAASLACGYLGMIERLQPTQPWEGSAHDAPYALPRS
                     VHEALRTLTSCDALKQVLGERFVTAFVSVKQAEYEAFFQVISSWEREYLLLNV"
     gene            2019..2522
                     /gene="greB"
                     /locus_tag="PLQ18_05950"
     CDS             2019..2522
                     /gene="greB"
                     /locus_tag="PLQ18_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020680694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HNE01231.1"
                     /translation="MSRYRPPAPASSPYITRAGYERLSAELQFLWKVRRPEVTRAVAE
                     AAAQGDRSENAEYTYGKKQLREIDRRVRYLSKRLDVLTVVDRIPDDRTRVYFGAWVEY
                     ETEAGETREVRIVGADEFDPAQHWISVDSPLARALLKRQDGDEVRVQLPEGEATVWIV
                     GIRYGEG"
     gene            2734..3168
                     /locus_tag="PLQ18_05955"
     CDS             2734..3168
                     /locus_tag="PLQ18_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007195293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF29 domain-containing protein"
                     /protein_id="HNE01232.1"
                     /translation="MAQARYEQDFAAWTAEQSRLLREGRLVELDLAHLAEEIEDMGKN
                     SQRALLSRLQELLLHLLKWQYQPGRRGNSWRASIRKQRQGIEDLLEDSPSLQPRLAAA
                     YGKCYARARLDAADETGLALTTFAESCPWSLDEVLDPAWLPD"
     gene            3509..>3778
                     /locus_tag="PLQ18_05960"
     CDS             3509..>3778
                     /locus_tag="PLQ18_05960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE01233.1"
                     /translation="MENRIAASLPADQLKTVLELIKQLEAALPFLMALSPDERKSLPR
                     MGDASRPFVDKSLKLAQAHNDFLPRSFDTGEFAADVQLYNALDTVR"
BASE COUNT          615 a         1361 c         1273 g          529 t
ORIGIN      
        1 cgccgcagta gcgcggactc gcgaccagct cgccgagttc gccggctgcg agccagcgca
       61 ggccgtcgta gccatagtcg tgcgctgcct gcgcctgcca gtcgcgcagc gcccgcacct
      121 gccgcggctt gagcgcgagc gtcgcgcagc cgtcgcgcca gtcgcaggcg atggcgtggc
      181 gggcgatgtg gttcttcacg cgcgcgaccg cctccagcga ccactgccag agctgcgcgg
      241 cagcctcgcg cccgagcagc ccgcgcagcg tttccatgcc gcaggcgtaa tcgccgagca
      301 cctgcccgcc gctgcgcccg gagccgccgg cgccggcgcg cgccgcctcg accagcacca
      361 cgcgcaggcc ccgcgccgcc agttcgagcg cggcgttgag gccggtgagg ccggcgccga
      421 gcacggcgac atcggcgcgc acgtcgccgg cgagtgccgg ggccggcggc tcgggaggca
      481 gcgcgtcctc gtaccaggag cgggcgtggg cgagcgtgcg catggcgtgg attcccccgg
      541 tcggcgcatt catcggcgcg ttcatcggcc tttgcagaag ccgggcagcc gctcgcggtg
      601 cgcgccttgt ttgcgcgcga gccgctcgac taggctcgcg gacggccgca gccgcggttt
      661 ccccggattc cccgcatgag ttccatcaag agctggttca aggaccatca gatcgtcgag
      721 gtcgaggcca tcatcccgga catgtccggg gtcgcgcgcg gcaagatcct gccggccgaa
      781 aagtattatc aggacaaggg cctgcgcctg cccgaaggca tcttcctgca gacgatcacc
      841 ggcgatgccc ccgacgatta ctacgacgtg atcagcgccg ccgacggcga catcgtgctg
      901 cgccccgacc tcgacaccat ctgcctcgtg ccctgggcga ccgagccgac cgcgcagatc
      961 atccacgacg cctattacgg cgacggccgc gtcgtcgaca tcgcgccgcg ccacgtgctg
     1021 cagcgcgtgc tcaggctcta cgccgagcgc ggctggaagc cggtcgtggc gcccgaactc
     1081 gaattcttcc tggtcaagcc gaacaccgat tcggattacc cgctggagcc gccgatcggg
     1141 cgctcgggcc ggcgcgaaac cggccggcaa tcctacggca tcgacgccgt caacgaattc
     1201 gagccgctgt tcgaggatgt ctacgactac tgcgagaagc agggcctcgg catcgacacg
     1261 ctgatccacg aatccggcgc cgcgcagctg gagatcaacc tgctgcacgg cgatgcgctc
     1321 gcgctcgccg atcaggcctt ccgcttcaag cgcacgatgc gcgaggcggc gatgcgtcac
     1381 cagatctacg cgaccttcat ggccaagccg atgcagcacg agcccggcag cgccatgcac
     1441 atccaccaga gcatcgtcgg cctcgacgac ggccgcaacg tgttcagcga cgaggacggc
     1501 aacccgacgc cgctgttcta cgcccacatc gccggcctgc agaagtacct gccgagcgcg
     1561 atggcgctga tcgcgccgaa cgtcaacagc taccggcgca tcaaccgcac cgatgtggcg
     1621 ccgatcaacg tgcagtgggg ctacgacaac cgcaccgccg ccttccgcgt gccgcaggcc
     1681 agcccgcagt cgcggcgcat cgagaaccgc gtgcccggtg ccgacgccaa cccctatctg
     1741 gcgatggccg cctcgctcgc ctgcggctac ctcggcatga tcgaacgcct gcagccgacc
     1801 cagccctggg agggcagcgc ccacgacgca ccgtatgcgc tgccgcgcag cgtgcacgag
     1861 gcgctgcgca cgctgacgag ctgtgacgcc ctgaagcagg tgctcggcga gcgcttcgtc
     1921 accgccttcg tctcggtcaa gcaggccgag tacgaagcct tcttccaggt catcagctcc
     1981 tgggagcgcg aatacctgct gctgaacgtc tgaaacccat gtcccgctac cgcccgcccg
     2041 ccccggcctc gtcgccgtac atcacccgcg ccggctacga gcgcctgagc gccgagctgc
     2101 agttcctgtg gaaggtcaga cgccccgagg tcacgcgcgc ggtcgccgag gccgccgcgc
     2161 agggcgatcg ctccgagaac gccgaataca cctacggcaa gaagcagttg cgcgagatcg
     2221 accggcgcgt gcgctacctc agcaagcgcc tcgacgtgct gaccgtcgtc gaccgcatcc
     2281 ccgacgaccg cacgcgcgtg tacttcggcg cctgggtcga gtacgagacc gaagccggcg
     2341 agacgcgcga ggtgcgcatc gtcggcgccg acgaattcga cccggcgcag cactggatca
     2401 gcgtcgactc gccgctcgcc cgcgcgctgc tcaagcgtca ggacggcgac gaggtgcgcg
     2461 tgcagctgcc ggagggcgag gcgacggtgt ggatcgtcgg gattcgctac ggcgagggct
     2521 gacggcgacg acggccatgc acggtacggg catgagcgca gcgtgcagga ggccggtgga
     2581 gcaaggtctt gtatcttctg accgtcctgc cgctggcctg cgcgtgcgtg ccgggccggt
     2641 ccggaccctg cctcgcgccg gcagcggcgg gaatctccgg ctgctgcgtc gtgaccggaa
     2701 tgaaattcga cgaaacctct gcgggaggct gtcatggctc aggcccgata cgagcaggat
     2761 tttgccgcct ggacggcgga gcagtcgcgg ctgctgcgcg aaggccgtct ggtcgaactc
     2821 gacctcgcac acctggccga ggagatcgag gacatgggca agaacagcca gcgcgccctg
     2881 ctgagccgcc tgcaggaact gctgctgcac ttgctgaagt ggcaatacca gccggggcgg
     2941 cgcggcaaca gctggcgggc ctcgatccgc aagcagcgtc agggcatcga ggatctgctc
     3001 gaagacagtc cgagcctgca gccgcgtctg gctgccgcct acgggaagtg ttacgcccgc
     3061 gcccgtctgg atgccgccga tgaaaccgga ctggcactga ccacgtttgc cgagtcctgc
     3121 ccgtggtctc tcgatgaggt gctggacccg gcctggctgc cggactgaaa gcacgcgaac
     3181 cgcgggcgga ctgcgtgcgc accggctgcg cgcagcttgc cgtgcgtgcg ggccgttcgg
     3241 cagcgccggc cgctgtggcg caagcggctg cccggcacgc gatatcggcg gacgcaaggg
     3301 catggcccgc agcccgggcc gggcacggcg caggccgttt tcaggcgggc gcaccgcaag
     3361 ccggcatcag gctccggacc gctgcgacaa agcgcgacga atacggcatc tgccggagtg
     3421 cgaggggccg ggtggctgcc gatactcgcg tccagcccgc gccgggcggt gcgggtcgtt
     3481 cgcagtgcca ccataaggag tgccgcacat ggaaaaccgt atcgccgcca gcctgcccgc
     3541 cgatcagctc aagaccgtac tcgagctgat caagcaactc gaagccgccc tgccgttcct
     3601 gatggcgctc agccccgacg agcgcaagag cctgccgcgc atgggcgatg ccagccggcc
     3661 gttcgtcgac aagtcgctga aactcgcgca ggcacacaat gattttctgc cgcgcagctt
     3721 cgacaccggc gagttcgccg ccgacgtgca gctttacaac gccctcgaca ccgtgcgc
//
LOCUS       DAOQJH010000209         3773 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_15099_length_3773_cov_5.363098, whole genome shotgun sequence.
ACCESSION   DAOQJH010000209 DAOQJH010000000
VERSION     DAOQJH010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3773
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15099_length_3773_cov_5.363098"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(169..744)
                     /locus_tag="PLQ18_05965"
     CDS             complement(169..744)
                     /locus_tag="PLQ18_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNE01234.1"
                     /translation="MQRTDFGAMSCPIARGLERVGEWWSMLILRDAFLGLSRFDEFQK
                     SLGIAPNMLARRLAHLVEAGLLEKHRYCQRPPRHEYLLTRRGEDFRPVLLTLLDFGNR
                     HYAPDGCRVQLVDTDTGEPVELALIDRRTGAEISRDRHRASPGPVDDADIGQRLAFSE
                     ALRSDPDNRPPYPGLPEPSPAAVPGPPETAS"
     gene            875..959
                     /locus_tag="PLQ18_05970"
     tRNA            875..959
                     /locus_tag="PLQ18_05970"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:909..911,aa:Leu,seq:tag)
     gene            1046..2374
                     /gene="tig"
                     /locus_tag="PLQ18_05975"
     CDS             1046..2374
                     /gene="tig"
                     /locus_tag="PLQ18_05975"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015259524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HNE01235.1"
                     /translation="MQVSVEELGGLERRLTVQLPAARIEQEIEQRLQDLSRRVKLDGF
                     RPGKVPLKVVKRMYGGQVRQEVLGELMQSSFQEAVSGQNLRPAAGPQISPSAAAEGDF
                     AYAATFEVLPEFELQGFEGIAVERPVAEVSDADIDSMLETLRKQRTVWTPVERPAQTG
                     DRVTVSFEGTIDGVAFPGGKGDEVPVVLGAGGMLPDFENGLIGIAAGEAREIAVSFPE
                     GYHAQELAGRTARFALNAGVVAEPSLPPVDEEFAKAFGVEDGSVESLRAALRDNMTRE
                     LGQGIKTRVKKQVMDALLAANPIELPKVLVTEEIDRIAQQAGFPAASAGNAEADKIKE
                     GVFAEEARRRVALGLLISKLVVQEKLTADPARVVEQLTTMASTYQDPNEVMQWYLKNP
                     QAMEGLRALALEDTVVDWVLARASVTEQPSTFDAVMRPAPAPAATADNAA"
     gene            2397..3032
                     /gene="clpP"
                     /locus_tag="PLQ18_05980"
     CDS             2397..3032
                     /gene="clpP"
                     /locus_tag="PLQ18_05980"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002690405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HNE01236.1"
                     /translation="MYPSKGGSDIQALNLVPMVVEQSARGERAYDIYSRLLKERVIFL
                     VGPVEDYMANLVVAQLLFLEAENPDKDIHLYINSPGGSVSAGMAIYDTMQFIRPQVST
                     MCVGQAASMGALLLAGGAKGKRYCLPHSRVMIHQPLGGFSGQASDIDIHAREILLIRD
                     RLNRVLAKHTGQPLERIAQDTDRDNFMGGEAAVEYGLIDSVLSQRSAPATA"
     gene            3263..>3773
                     /locus_tag="PLQ18_05985"
     CDS             3263..>3773
                     /locus_tag="PLQ18_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014777181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpX C4-type zinc finger protein"
                     /protein_id="HNE01237.1"
                     /translation="MSKDRNDKGDDGKLLYCSFCGKSQHEVRKLIAGPNVFICDECVE
                     LCNDIIREEMEEKTPSAGSKLPKPHEIKKVLDEYVIGQDQAKKVLSVAVYNHYKRLEL
                     RRGKDEVEVSKSNILLIGPTGSGKTLLAETLARLLNVPFTIADATTLTEAGYVGEDVE
                     NIIQKLLQKC"
BASE COUNT          639 a         1231 c         1329 g          574 t
ORIGIN      
        1 cgaggtgctt gtcgaggtgc aggacgatgt cgagaaactg ggcgagtaat tccatgcgcg
       61 ggtccggagc ggaagcggaa catccatgct acctgccgtg tgcgctgcag cacaggcacc
      121 ggccgggccg cgggcgtgcg gccggggtct ggcggtgccg gcggcggctt acgatgcggt
      181 ttcaggcgga cccggcacgg ctgccggtga cggttcgggg aggccggggt agggcgggcg
      241 gttgtccggg tcgctgcgca gggcttcgga gaaggccagc cgctggccga tgtcggcatc
      301 gtccaccggg ccggggctgg cgcggtggcg gtcgcggctg atctcggcgc cggtgcggcg
      361 gtcgatcagg gccagctcca cgggctcgcc ggtgtcggtg tcgaccagct gcacccggca
      421 gccgtccggc gcgtagtgcc ggttgccgaa atcgagcagc gtcagcagca ccggccggaa
      481 atcctcgccg cggcgggtca gcaggtattc gtggcggggc gggcgctggc agtagcggtg
      541 cttttcgagc aggccggcct cgaccagatg cgccagccgc cgcgccagca tgttcggagc
      601 gatgccgagg ctcttctgga actcgtcgaa gcggctcagg cccaggaatg cgtcgcgcag
      661 gatcagcatg ctccaccatt cgccgacccg ttcgaggccg cgggcgatcg ggcaggacat
      721 ggcgccgaaa tcggtgcgct gcatgggaag tgggctcggg cggcggcggt cggccggtga
      781 ctttatggcc gctgccgccc ggcgggcaag gctgcggccg ggatgttttt gtgtatgatc
      841 caccggcttt cgccgcaccc ggcggtgaac cgacgcgaaa gtggcgaaac tggtagacgc
      901 gctggattta ggttccagtg gggcaacccg tgagagttcg agtctctcct ttcgcaccag
      961 tgtccagcga ggtccgcgcg cgcccgtgca caggcgatgc gacgcgggcc ttttcgcttc
     1021 gttccggatg atcaggggta cccagatgca ggtttcagtt gaggagctcg gcggtctcga
     1081 gcgtcggctc acggtgcagc ttcccgcggc caggatcgag caggagatcg aacagcgcct
     1141 gcaggatctc tcacgccgcg tgaagctcga cggcttccgt cccggcaagg tgccgctgaa
     1201 ggtcgtcaag cgcatgtacg gcggccaggt ccggcaggaa gtcctgggtg agctgatgca
     1261 gtcgagcttc caggaagccg tcagcggcca gaacctgcgc ccggccgccg gcccgcagat
     1321 ttcgccgtcc gcagccgccg aaggtgactt cgcctatgcg gcgaccttcg aggtcctgcc
     1381 cgaattcgag ctgcagggct tcgaaggcat tgccgtcgag cgcccggtcg ccgaggtcag
     1441 cgacgccgac atcgactcga tgctcgaaac cctgcgcaag cagcgcaccg tgtggacacc
     1501 ggtcgagcgc ccggcgcaga ccggcgatcg cgtcaccgtc agcttcgagg gcacgatcga
     1561 tggtgtcgcc ttcccgggcg gcaagggcga tgaggtgccg gtcgtgctcg gggccggcgg
     1621 catgctgccg gacttcgaga acggcctgat cggcattgcg gccggcgagg cgcgcgagat
     1681 cgccgtgagc tttcccgagg gctatcacgc gcaggaactc gccggcagga cggcgcgctt
     1741 cgcgctgaat gccggtgtcg tcgccgagcc gagcctgccg ccggtcgacg aggagttcgc
     1801 gaaggccttc ggcgtcgagg acggcagcgt cgagtcgctg cgcgcggcgc tgcgcgacaa
     1861 catgacgcgc gaactgggcc agggcatcaa gacccgcgtc aagaagcagg tcatggacgc
     1921 actgctggcc gcgaacccga tcgaactgcc gaaggtgctg gtcaccgagg agatcgaccg
     1981 catcgcgcag caggccggct tcccggcggc cagtgccggc aacgccgagg ccgacaagat
     2041 caaggagggg gtgttcgccg aggaggcccg ccggcgcgtc gcgctcggcc tgctgatctc
     2101 gaagctggtc gtgcaggaga agctgaccgc cgatccggcg cgcgtcgtcg aacagctgac
     2161 gacgatggcc tcgacctatc aggacccgaa cgaggtcatg cagtggtacc ttaagaatcc
     2221 gcaggcgatg gaaggcctgc gtgcgctggc gctggaggac accgtggtcg actgggtgct
     2281 cgccagggcc agcgtcaccg agcagcccag cactttcgat gcggtgatgc gcccggcccc
     2341 ggcaccggcc gccaccgccg acaacgccgc ctgacccgcg gcaggagaca ggtgacatgt
     2401 acccgagcaa gggcggttcc gacatccagg cactgaatct ggtgccgatg gtggtcgagc
     2461 agtcggcacg cggcgaacgc gcgtacgaca tctactcgcg cctgctgaag gagcgcgtga
     2521 tcttcctggt cgggccggtc gaggattaca tggccaacct cgtggtggcg cagctgctgt
     2581 tcctcgaagc cgagaatccg gacaaggaca tccacctgta catcaactcg cccggcggct
     2641 cggtcagtgc cggcatggcg atctacgaca ccatgcagtt catccggccc caggtcagca
     2701 cgatgtgcgt cggccaggcg gccagcatgg gggcgctgct gctcgccggc ggcgccaagg
     2761 gcaagcgtta ctgcctgccg cactcgcggg tcatgatcca ccagccgctc ggcggcttca
     2821 gcggccaggc ctcggacatc gacatccacg cccgcgagat cctgctgatc cgtgaccgcc
     2881 tgaaccgcgt gctcgccaag cacaccggcc agccgctcga acgcatcgcc caggacaccg
     2941 accgtgacaa tttcatgggc ggtgaggcag cggtcgaata cgggctgatc gattcggtgc
     3001 ttagccagcg cagcgctccg gccaccgcct gactcctgca ccgacagccc gcccccggtc
     3061 cggctccctc cggccggggg cggtgcgttt ccttgccatg accggacggg cgtagttgaa
     3121 tacgaccgaa cgggctggtc cccttcaggt cacattcctg ctgtttccat ccgcacggcg
     3181 cactgtctgc gccgggcctc cgtaccggca cggggccgat cgcggcgcac atcagggccg
     3241 cccaccgcgt gaggatcgca ccatgagcaa agaccgaaac gacaagggcg atgacggcaa
     3301 gctcttgtac tgctcgttct gtggcaagag ccagcacgaa gtccgcaagc tgatcgccgg
     3361 tcccaacgtc ttcatctgcg acgaatgcgt cgagctctgc aacgacatca tccgcgagga
     3421 gatggaggag aagacgccga gcgccggcag caagctgccg aagccgcacg agatcaagaa
     3481 ggtcctcgac gagtacgtga tcggccagga ccaggccaag aaggtcctgt cggtcgccgt
     3541 ctacaaccac tacaagcgcc tcgaactgcg ccgtggcaag gacgaggtcg aggtctccaa
     3601 gtccaacatc ctgctgatcg gcccgaccgg ttccggcaag acgctgctcg ccgagacgct
     3661 ggcgcggctg ctgaacgtgc cgttcacgat cgccgatgcg acgacgctga ccgaagccgg
     3721 ctacgtcggc gaggacgtcg agaacatcat ccagaagctg ctgcagaagt gcg
//
LOCUS       DAOQJH010000210         3773 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_15107_length_3773_cov_2.983593, whole genome shotgun sequence.
ACCESSION   DAOQJH010000210 DAOQJH010000000
VERSION     DAOQJH010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3773
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15107_length_3773_cov_2.983593"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..704)
                     /locus_tag="PLQ18_05990"
     CDS             complement(<1..704)
                     /locus_tag="PLQ18_05990"
                     /inference="COORDINATES: protein motif:HMM:NF013646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNE01238.1"
                     /translation="MGNTAASPVSTASPASTAAAAAAPGTVYDVIVVGGGMVGSALAC
                     ALGEHGLDVALIEAEPVPPLMAGEPAAARVSAISAVSQRILARLGAWPAIEQLPGAGG
                     GAPEPRVCAYTDMEVWDASGPGLIHFDAAGIGEPALGWIIENRVIQSALLARAATIDA
                     LDVYPGVAVTGFEEGRNALGVRLADGRRLSARLLVGADGAQSPLRAWAGIDTMGWGYE
                     QQAVVCRVRCEQPHHDT"
     gene            complement(753..2078)
                     /gene="ubiH"
                     /locus_tag="PLQ18_05995"
                     /gene_synonym="visB"
     CDS             complement(753..2078)
                     /gene="ubiH"
                     /locus_tag="PLQ18_05995"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018233628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008681 - 2-octaprenyl-6-methoxyphenol
                     hydroxylase activity [Evidence IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HNE01239.1"
                     /translation="MGSKRSEDTAVTGSGPVVREDMAPAAAGASAAGAAGDYDVIVVG
                     GGMAGAALAIALDGLGLKLALVEASPWHADAHPGYDERTLALSWGSRRIFDGLGVWTA
                     IAAQATPIRQIDISDRGHIGHARLDARDQGTPALGYVAAARTIGAALIGRLERLAHVT
                     LLCPAELEHLSPGAQHCEVTVRVGEQLHSLRARLVVAADGARSRIRTQLGVPTLEWSY
                     GQTAVTANVTPEHDHGNVAYERFTEDGPLALLPMEGGRCALVLTVPDAEVEPVLAMPD
                     AQFQYLVQRRFGLRLGRFVRAGRRSAHPLKLVKAREHRHGRVAFIGNAAHTLHPIAGQ
                     GFNLGLRDVAVLAEVIADALAADEDPGSSAVLTRYADWRRWDQRATIGFTDALVRVFA
                     NPLPPVRLARNLGLIGFDLLPPVKRLLVRQLMGVAGRQPRLGLGRALAG"
     gene            complement(2197..3516)
                     /gene="pepP"
                     /locus_tag="PLQ18_06000"
     CDS             complement(2197..3516)
                     /gene="pepP"
                     /locus_tag="PLQ18_06000"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011630275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HNE01240.1"
                     /translation="MASFRIPAEEYAARRARLMAAMGEGSIALVAAATERVRNHDVHY
                     PFRQDSDFRYLTGFPEPDALAVLRPGHAESYVLFCRPRDPLMEIWHGRRAGPAGAIER
                     FGADAAYPLDELDQRLPELLEGVERVHYRYGADPALDTRVSGWISALRAKLRSGVHVP
                     RELVNLDALLHAHRRVKSPAELEVMRSAGRISAAGHRRAMQVCRPGLNERRLEAEILH
                     EFALAGAGWSYPAIVGGGDNACILHYTENDAELRDGDLVLIDAGCELDSYAGDITRTF
                     PVNGRYSGPQRALYEVVLAAQHAAMAVVRPGMAWPDYHDAAVRVLTEGLISLGLLEGG
                     VDERIADESYRRFYMHRTGHWLGMDVHDPCEYKDGADWTRLEAGMVLTVEPGLYVAPD
                     AEGVDARFRGIGIRIEDDVAVTADGCEVLSAGVPTAIDDIEALMRDA"
     gene            complement(3587..>3773)
                     /locus_tag="PLQ18_06005"
     CDS             complement(3587..>3773)
                     /locus_tag="PLQ18_06005"
                     /inference="COORDINATES: protein motif:HMM:NF015642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UPF0149 family protein"
                     /protein_id="HNE01241.1"
                     /translation="AREVLDDLREFAQADYETDDADEEGEAAWVELVEYLRIGVLLLY
                     ATLAAPAPAAESGRRLH"
BASE COUNT          517 a         1389 c         1379 g          488 t
ORIGIN      
        1 gtgtcatggt gcggctgctc gcagcgcacg cgacagacca cggcctgctg ctcgtagccc
       61 cagcccatcg tgtcgatgcc ggcccaggcg cgcagcggcg actgcgcgcc gtcggccccg
      121 accagcaggc gcgccgacag ccggcggccg tcggccagcc gcacgccgag ggcattgcgc
      181 ccttcctcga agccggtgac ggcgacgccg gggtagacat cgagcgcgtc gatcgtggcg
      241 gcgcgcgcga gcagcgcgct ctggatcacg cggttctcga tgatccagcc gagggccggc
      301 tcgccgatgc cggcggcatc gaagtggatc aggcccgggc cgctcgcgtc ccagacctcc
      361 atgtcggtat aggcgcagac ccgcggctcg ggtgcgccgc ccccggcccc cggcagctgc
      421 tcgatcgccg gccaggcgcc gagtctggcg aggatgcgct gcgagacggc actgatcgcc
      481 gagacgcgcg ccgcggccgg ctcgccggcc atgagcggcg gcaccggttc ggcctcgatc
      541 agtgccacgt ccagcccgtg ctcgccgagc gcgcaggcca gcgccgagcc gaccatgccg
      601 ccgccgacga cgatgacgtc gtagacggtc ccgggggcgg ctgcggcagc ggccgtggat
      661 gcaggggaag cagtggaaac aggcgaagca gcggtgttgc ccatcggatg cggaggttcc
      721 gtgcagtcag gcggaatcgg ggagcgggac gttcagccgg ccagggcccg gccgaggccg
      781 agccgcggct ggcgaccggc gacgcccatc agctggcgca ccagcaggcg cttgaccggc
      841 ggcagcaggt cgaagccgat caggccgaga ttgcgcgcca ggcgcaccgg cggcagcggg
      901 ttcgcgaaca cgcgcaccag cgcatcggtg aagccgatcg tcgcgcgctg gtcccagcgc
      961 cgccagtcgg cgtagcgcgt cagcacggcg ctgctgccgg ggtcctcgtc ggcggccagc
     1021 gcatcggcga tgacctcggc cagcaccgcg acatcgcgca ggccgaggtt gaagccctgc
     1081 ccggcgatcg ggtgcagcgt gtgtgcggca ttgccgatga aggcgacgcg cccgtgccgg
     1141 tgctcgcgcg ccttgacgag cttcagcgga tgggcgctgc gccggccggc gcgcacgaag
     1201 cgcccgagcc ggaggccgaa gcgccgctgc accaggtact ggaactgcgc atccggcatc
     1261 gccagcaccg gctcgacctc ggcatcgggc acggtcagca ccagcgcgca gcgcccgcct
     1321 tccatcggca gcagcgcgag cggcccgtcc tcggtgaagc gctcgtaggc gacattgccg
     1381 tggtcgtgct cgggcgtgac gttggcggtc accgccgtct ggccgtagct ccactccagc
     1441 gtcggcacgc cgagctgcgt gcggatgcgc gagcgcgccc cgtcggccgc gaccaccagc
     1501 cgcgcgcgca gcgagtgcaa ctgctcgccg acgcgcaccg tcacctcgca gtgctgcgcg
     1561 ccggggctca gatgttcgag ttcggccggg cagagcagcg tcacgtgtgc aagccgctcc
     1621 agccgcccga tcagcgccgc cccgatcgtg cgcgccgcgg ccacgtagcc gagcgccggc
     1681 gtgccctggt cgcgggcatc gagccgggcg tggccgatgt ggccgcggtc ggagatgtcg
     1741 atctgccgga tcggcgtcgc ctgcgccgcg atcgccgtcc agacgccgag cccgtcgaag
     1801 atgcgccgcg agccccagga cagcgccagc gtgcgctcgt cgtagccggg gtgagcatcg
     1861 gcgtgccagg gactggcctc gaccagtgcg agcttcaggc ccagcccgtc gagcgcgatc
     1921 gccagcgccg caccggccat gccgccaccg acgacgatga cgtcgtaatc cccggcggct
     1981 ccggcggcag acgcgccggc cgctgccggc gccatgtcct cgcggacgac cggcccgctg
     2041 ccggtcaccg ccgtgtcttc gctgcgcttg ctgcccacgc ctcgctcctc gggtccgccg
     2101 ccgtccggcg ccggtgatgg gtccgctcgc accggaaaaa ccggccggcg gcgcttgtcg
     2161 ttgtcaggtc cgggcgctgc gctgcgcacg ccgtgctcag gcgtcgcgca tcagcgcctc
     2221 gatgtcgtcg atcgcggtcg gcacgccggc gctcagcacc tcgcagccgt cggccgtgac
     2281 cgccacgtca tcctcgatgc ggatgccgat gccgcgaaag cgcgcatcga cgccttcggc
     2341 atcgggggcg acgtacaggc ccggctcgac ggtcagcacc atgccggctt cgagccgcgt
     2401 ccagtcggcg ccgtccttgt attcgcaggg atcgtgcacg tccatgccga gccagtggcc
     2461 ggtgcggtgc atgtagaagc gccggtagga ctcatcggcg atgcgctcgt cgaccccgcc
     2521 ttcgagcagg ccgaggctga tcaggccctc ggtcagcacg cgcacggcgg cgtcatggta
     2581 gtcgggccag gccatgccgg ggcgcacgac ggccatcgcc gcatgctgcg cggccagcac
     2641 gacctcgtac agcgcgcgct gcggcccgct gtagcggccg ttgaccggga aggtgcgggt
     2701 gatgtcgccg gcgtaggagt ccagctcgca gccggcgtcg atcagcacca gatcgccgtc
     2761 gcgcagctcg gcgtcgttct cggtgtaatg caggatgcag gcgttgtcgc cgccgccgac
     2821 gatcgccgga tacgaccagc cggcgccggc gagcgcgaac tcgtgcagga tctcggcctc
     2881 cagccgccgc tcgttcaggc ccggccggca gacctgcatc gcgcgccggt gcccggccgc
     2941 cgagatgcgc ccggccgagc gcatgacctc cagctcggcc ggcgacttca cgcgccggtg
     3001 cgcgtgcagc agcgcatcga ggttcacgag ttcgcgcggc acgtgcacgc cgctcctgag
     3061 cttcgcgcgc agcgcgctga tccagccgct gacgcgggtg tcgagcgccg gatcggcgcc
     3121 gtagcggtaa tgcacgcgct cgacgccttc gagcagctcc ggcaggcgct ggtcgagctc
     3181 gtcgagcgga tacgcggcat cggccccgaa gcgctcgatc gcgccggccg gcccggcgcg
     3241 gcggccgtgc cagatctcca tcagcggatc gcgcggccgg cagaacagca cgtaggactc
     3301 ggcgtggccg gggcgcagca ccgccagcgc gtcgggctcc gggaagccgg tcaggtagcg
     3361 gaagtcgctg tcctggcgaa acggatagtg cacgtcgtgg ttgcgcacgc gctcggtcgc
     3421 cgcggcgacc agcgcgatcg aaccctcgcc catcgccgcc atcagccggg cgcgacgggc
     3481 ggcatattcc tcggccggaa tgcggaagct ggccatcggc tgacctcgga gtgcaggtgg
     3541 gaaacgaaac ggaatgccgg acaccggcag cgtcggtgcc ggcgaatcag tgcagacgcc
     3601 tgccggattc ggccgccggg gccggcgcgg cgagcgtcgc gtacagcagc agcacgccga
     3661 tgcgcaggta ctcgaccagc tcgacccacg ccgcctcgcc ttcctcgtcg gcgtcgtcgg
     3721 tttcgtagtc ggcctgcgcg aactcgcgca ggtcatcgag cacctcgcgc gcc
//
LOCUS       DAOQJH010000211         3763 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_15167_length_3763_cov_3.322546, whole genome shotgun sequence.
ACCESSION   DAOQJH010000211 DAOQJH010000000
VERSION     DAOQJH010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3763)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3763)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3763
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15167_length_3763_cov_3.322546"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1569
                     /gene="prsT"
                     /locus_tag="PLQ18_06010"
     CDS             <1..1569
                     /gene="prsT"
                     /locus_tag="PLQ18_06010"
                     /inference="COORDINATES: protein motif:HMM:TIGR02917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM system TPR-repeat protein PrsT"
                     /protein_id="HNE01242.1"
                     /translation="GRYAYGMSLLAAGDQNRGLEELQKAVELDPHEHGLEFGLSVSLL
                     RAGRFDEAIAVAHRLQQSLPDQAEPLVLEGIAQAARQQDAEARAAFRRALALEPGHPR
                     AAGALAALDLGSGDIDGARGWYRQILAHHPGHAPTLMQLARLEARQGQGEAYAETLRE
                     AIAADPHVPQPYVLLARWQLRQGQPLLAVGTLVAVRAEFPEYPPLLLTLGLAQGANGE
                     SADAIGTLRRLIELQPQSAEARYALVTAHAQAGDAAGLRSTLAEALAVDNRRPEALPL
                     LERLVGLAGSASEAEDWLEALLRDRPDDEIVLDFIGQRRYRQYGAPTALTYYAQLQRR
                     QPQAALWVLREAELRSGNREAAQAQALLERWLQAHPDDVQARFALGLIHLDADRRAPA
                     RAAFEAVLERRPDQLAALNNLAWLLREDDPQRALDYANRCLQLRPADPRLMDTLGEVL
                     IAQHQYARAAEVLAKAVARAPQLGSARYHLALALSLDGRRDEARELLKSLLGNDQPFS
                     ERAEAQSLLAQIGS"
     gene            1679..>3763
                     /gene="prsT"
                     /locus_tag="PLQ18_06015"
     CDS             1679..>3763
                     /gene="prsT"
                     /locus_tag="PLQ18_06015"
                     /inference="COORDINATES: protein motif:HMM:TIGR02917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM system TPR-repeat protein PrsT"
                     /protein_id="HNE01243.1"
                     /translation="MRPLAAALLLLAGSATAAADLARELDKAEQLRAQGQLGAAIIEL
                     KNALQDEPDYGPGRRLLGVLYVELGNGAEAEKELLRARRLKVRETGIALPLAEALLLQ
                     REYQRLLESVQPVSGMSMRDVADLRALRGEALLALGRQGDAEAAVKSALATDPAAPRA
                     QIAEARLALAARHPDTARQRLQTVLSAPLPQPVEHIAQALLGDVEIMARRLPEAAKAY
                     DRAIATGRPHQALAIQLKRGLLRIDLGDPDGARADLGAVNARLPDLPLPHFGFGLLAY
                     RQGRYADALDSFERALQRDHENPDIHAKIAATRIALGQREQAIADLEKFLQEHPDSDP
                     ILRLLATIRLQQGDTAGAQKLLRHVLELHPDDNAVLDMLGAIALARGEAREGEPLLRR
                     SVERDPDSAAARYRLGLGLLALGDRDNGLRELAQATQLQPGQERYEYALALGLLRSGQ
                     HAEAVAAAQRLQTLWPDRNEPLLLEGIARASALDTEGASAAFERVLARSPGDPAASGG
                     LATLDLQQGRPDKARSRYRAVLEQHPGHGATLLELAALELRLDNPEAARRALEQAVAA
                     DPEALEPRLRLARLLGREGRHAAALELLEAVHARHADDVQLLALLGAAKTALGDAGGG
                     RAAYRRLLDLDPKSATGLYGYASACAAGSDDAGLRAALRPALAADPGNALAQALLARL
                     QELAPGEAAARAW"
BASE COUNT          527 a         1457 c         1320 g          459 t
ORIGIN      
        1 ggccgctatg cctacggcat gagcctgctc gccgccggcg accagaaccg cggcctggag
       61 gagctgcaga aagccgtcga gctcgatccg cacgagcacg gtctggagtt cggcctgagc
      121 gtcagcctgc tgcgcgccgg gcgcttcgac gaggccatcg ccgtcgcgca ccgcctgcag
      181 cagagcctgc cggaccaggc cgagccgctg gtgctcgaag gcattgccca ggccgcccgc
      241 cagcaggatg ccgaagcccg tgccgccttc cgccgggcac tggcactgga gcccggacac
      301 ccgcgggccg ccggcgccct cgccgccctg gacctcggca gcggagacat cgatggcgcg
      361 cgcggctggt accggcagat cctcgcccat caccccgggc atgccccgac gctgatgcag
      421 ctcgcccggc tcgaagcccg gcagggccag ggcgaggcct atgccgaaac cctgcgcgaa
      481 gccatcgccg ccgatccgca tgtgccgcag ccctacgtgc tgctcgcccg ctggcagctg
      541 cgtcagggcc agcccctgct cgccgtcggc accctggtcg cggtacgcgc cgagtttccg
      601 gagtatccgc cgctgctgct gacgctcggc ctggcacagg gcgcaaacgg cgaaagcgcc
      661 gatgcgatcg gcacgctgcg ccggctgatc gaactgcagc cgcagtcggc cgaggcccgc
      721 tacgcgcttg tgaccgcgca cgcgcaggcc ggcgatgccg cgggcctgcg cagcacgctc
      781 gcggaagccc tggccgtcga caaccgccgc cccgaagccc tgcccctgct cgaacgcctg
      841 gtcggtctcg ccggcagcgc cagcgaggcc gaggactggc tcgaagcgct gctgcgcgat
      901 cgcccggacg acgagatcgt gctcgacttc atcggccagc gccgctaccg gcaatacggc
      961 gccccgaccg cgctgaccta ctacgcccag ctgcagcgcc gccagccgca ggccgcgctg
     1021 tgggtgctgc gcgaggccga gctccgcagc ggcaaccgcg aggccgcgca ggcccaggcc
     1081 ctgctcgaac gctggctgca ggcacacccg gatgacgtgc aggcgcgctt cgcgctcggc
     1141 ctgatccacc tcgacgccga tcgccgggcc ccggcccgcg ccgccttcga ggcggtgctc
     1201 gaacgccggc ccgaccagct cgcggccctg aacaacctgg cctggctgct gcgcgaggac
     1261 gatccgcagc gggcgctgga ctacgcgaac cgctgcctgc agctgcgccc ggccgatccg
     1321 cggctgatgg acacgctcgg cgaggtgctg atcgcgcagc accagtacgc ccgggccgcc
     1381 gaggtgctgg cgaaggccgt cgcgcgcgcc ccgcagctgg gctcggcccg ctatcacctg
     1441 gcacttgcgc tgtcgctcga cggacgcagg gacgaggccc gggagctgct gaaatccctg
     1501 ctcgggaatg atcagccctt ctccgagcgc gccgaggcgc agagcctgct cgcgcagatc
     1561 ggcagctgaa tccgctccgg ttcaccgggt cgccggcaag ggccaggggg ccggccggcg
     1621 gccgccgggt ttcatcggct tcatcgatcg agggacttgc atgatgtgga acacccggat
     1681 gcggccgctt gcggctgcgc tgctgctgct cgccggcagc gcaaccgccg cagccgacct
     1741 ggccagggaa ctggacaagg ccgaacagct gcgcgcgcag ggccagctcg gcgccgcgat
     1801 catcgaactg aagaacgcgc tgcaggacga gcccgattac gggccgggcc ggcggctgct
     1861 cggcgtgctg tacgtcgagc tcggcaacgg tgcggaggcg gaaaaggaac tgctgcgcgc
     1921 ccggcggctg aaggtccgcg agaccggcat cgcactgccg ctcgccgaag ccctgctgct
     1981 gcagcgcgaa taccagcgcc tgctcgaaag cgtgcagccg gtctcgggca tgtcgatgcg
     2041 cgatgtcgcc gacctgcgcg cgctgcgcgg cgaggcgctg ctcgcgctcg gccgccaggg
     2101 cgacgccgag gcggccgtga agtccgcgct cgccaccgac ccggccgcgc cgcgtgcgca
     2161 gatcgccgag gcgcgtctcg cactcgcggc ccgccacccc gacaccgccc gccagcgcct
     2221 gcagaccgtg ctgtcggccc cgctgccgca gccggtcgag cacatcgcgc aggcgctgct
     2281 cggcgatgtc gagatcatgg cccggcgcct gccggaggcc gcgaaggctt acgaccgcgc
     2341 cattgccacc ggccggccgc accaggcgct ggcgatccag ctcaagcgcg ggctgctgcg
     2401 catcgatctc ggtgatcccg acggtgcccg tgccgatctg ggtgcggtca acgcccggct
     2461 gccggatctg ccgctgccgc atttcggctt cgggctgctc gcgtaccggc aggggcgcta
     2521 tgccgatgcg ctggattcct tcgaacgtgc gctgcagcgc gatcacgaga atcccgacat
     2581 ccacgccaag atcgccgcca cgcgcatcgc gctcggccag cgcgagcagg cgatcgcgga
     2641 tctggaaaaa ttcctgcagg aacatccgga cagcgatccg atcctgcgcc tgctcgcgac
     2701 gattcgcctg cagcagggcg ataccgccgg cgcgcagaaa ctgctgcgcc acgtgctcga
     2761 actgcatccc gacgacaacg ccgtgctcga catgctcggc gcgatcgcgc tcgcccgcgg
     2821 cgaagcgcgc gagggcgagc cgctgctgcg ccgctcggtc gaacgcgatc cggactcggc
     2881 cgcggcgcgc taccggctcg gcctcggcct gctggcactc ggcgatcgcg acaacggcct
     2941 gcgcgaactc gcgcaggcca cccagctgca gcccggccag gaacgctacg aatacgcgct
     3001 ggcactcggg ctgctgcgca gcggccagca cgccgaagcc gtcgccgccg cccagcgcct
     3061 gcagacgctg tggccggatc gcaacgaacc gctgctgctc gaaggcatcg cccgcgcgag
     3121 cgccctcgac accgagggcg ccagtgccgc cttcgagcgc gtgctcgcgc gcagccccgg
     3181 cgacccggcc gcatcgggcg gactggcgac gctcgacctg cagcagggcc ggcccgacaa
     3241 ggcccgctcg cgctatcggg ccgtgctcga acagcatccg ggccatgggg ccacgctgct
     3301 ggagctggca gcgctcgaac tgcggctcga caaccccgag gccgcccggc gcgcgctgga
     3361 gcaggcggtc gcggccgatc cggaggcgct ggagccgcgc ctgcgcctcg cgcgcctgct
     3421 cgggcgcgag ggccggcatg cggcggcgct cgaactgctc gaagccgtgc atgcccgcca
     3481 tgcggacgat gtgcagctgc tcgcgctgct cggagccgcg aagaccgcac tcggggatgc
     3541 cggcggcggc cgcgccgcct accgccgtct gctcgatctc gatccgaagt ccgccaccgg
     3601 cctgtacggc tacgcctcgg cctgcgccgc cggcagcgac gacgccggcc tgcgcgcggc
     3661 gctgcgtccg gcgctcgccg ccgaccccgg caatgccctc gcgcaagcgc tgctggcacg
     3721 gctgcaggaa ctggccccgg gcgaggccgc cgcccgcgcc tgg
//
LOCUS       DAOQJH010000212         3760 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_15184_length_3760_cov_4.401080, whole genome shotgun sequence.
ACCESSION   DAOQJH010000212 DAOQJH010000000
VERSION     DAOQJH010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3760)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3760)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3760
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15184_length_3760_cov_4.401080"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            386..1369
                     /locus_tag="PLQ18_06020"
     CDS             386..1369
                     /locus_tag="PLQ18_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HNE01244.1"
                     /translation="MTIKSHANVGVYVDAANISRNGGSRMQYDVLREFACRDYAEALR
                     LNVYLTFDEDRANSNYTYRERMRAYQAALRELGYKVIEKKVRWFEDESGTRYGKANAD
                     LDMAVDLLLQSQNLDRVVLVTGDGDFVQVVRALQNKGCRVEVIGFDNVSEDLRNEADL
                     YVSGFLIPGLLPARGEDTGVPWGEIGSRVRGYCYHHDENKSFGFMRFLVRSSPYLWKT
                     DSRDPDSPYRTAFFHDSNLPDGFNVMRLPSRHHVFEFTLAEPSGKQPVATDIQLVCPP
                     MAERGWRGSSRHGASSSAAAAASTAAEAEGSAGHDDDQDADDGDHPNGNVA"
     gene            1366..3462
                     /locus_tag="PLQ18_06025"
     CDS             1366..3462
                     /locus_tag="PLQ18_06025"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HNE01245.1"
                     /translation="MRAARMAPCRLSLAAALLLSFATVACAATVGDALPPLPKTLPDL
                     SPDTVQSSVDKTVAGLLTQYHYRRVKIDSALSATVFDDYLSALDFSHSFLLASDVAEL
                     GQYRDKIDDELRNGRLDAAFAIYNLYQQRLLERTRRISEELTRPFDFRKDETYEADRK
                     KAPWARTAAELDEIWRKRLKNETLSLMLAGKDEAAARDTLRKRYEGRLRRAAQSGPED
                     VFQIYMNAVTGAFDPHTSYLSPRNSENFNIQMRLSLEGIGAVLRLEDEQTVVVELVPG
                     GPADLSKQIRPKDRILAVGQGDDGAMTDVVGWRLDDVVDLIRGQRGSVVRLQVVPAQA
                     AAGSPGTVVRIVRNKIQLEEQAAKSQVKTFVRDGVERKVGVITIPTFYIDFAAAQRGD
                     LDYRSTTRDVRRLIEQLQRERVEGIVIDLRQNGGGSLQEAVELTGLFIADGPVVQVRD
                     AKGKVQVENDPDPKQFYSGHLAVLTDRFSASASEIFAAAIQDYGRGVLVGEQTFGKGT
                     VQTLIDLGRFLPQVKDKLGQLKLTIAKFYRINGESTQNRGVLPDVALPPAFATDEVGE
                     SAQEHALPWDEIPPTRYKVAGRVRDGVTELKRLHEARVPHDQPYQDLKHDLEVSMALR
                     KQTTVSLLESKRRTDQEASKSRMRTARGRVIDPDSDDAGDAKKDGPDPFLDETGQILL
                     DWIDLRAGRIAQAPAD"
BASE COUNT          673 a         1259 c         1246 g          582 t
ORIGIN      
        1 ttctgattta tccgggctat tcaggcaagt ccggaaaccg atactgcaag ttgtgtagat
       61 acctatgccc cgcgaggggg ggagggcttg ctcatcagcg tcttgccgtc gtgctgatcc
      121 atccgtgctg gctggacaag tccgggaacc gctgctgcaa gttgtgtaga taccgaatgg
      181 ccggtgcagg caagggcgcc gaaatccggc gtcctgcgaa caagcggttc ccgggggatt
      241 ctgcgacaat cagccggccc gcatcggccg gcatgcagcc acggcgggct gtccggcctg
      301 cagggtttcg ccttcatctt tgtcatcatt tgtcgtatcc cgcttttcgc cggcctcacc
      361 ggcgcgggat catcgaggta gcttcgtgac catcaagtct cacgccaacg tcggcgttta
      421 cgtcgacgcc gccaatattt cccgcaacgg cggcagccgc atgcagtacg acgtgctgcg
      481 tgaatttgcc tgccgcgatt acgccgaggc gctgcgcctg aatgtctatc tgaccttcga
      541 cgaggatcgg gccaacagca actacaccta ccgtgaacgg atgcgcgcgt atcaggcggc
      601 gttgcgcgaa ctcggctaca aggtcatcga aaagaaggtc cgctggttcg aggacgagtc
      661 cggcacccgc tacggcaagg ccaacgccga tctcgacatg gccgtcgacc tgctgctgca
      721 gtcgcagaat ctcgatcgcg tcgtgctcgt gaccggcgac ggcgatttcg tgcaggtggt
      781 gcgggcgctg cagaacaagg gctgccgggt cgaggtcatc ggcttcgaca acgtctcgga
      841 agacctgcgc aacgaggccg acctgtacgt ctcgggcttc ctgatccccg gcctgctgcc
      901 ggcgcgcggc gaggataccg gcgtgccgtg gggcgagatc ggctcgcggg tgcgtggcta
      961 ctgctatcac cacgacgaga acaagagctt cggcttcatg cgcttcctgg tgcgcagctc
     1021 gccgtacctc tggaagaccg actcgcgcga ccccgattcg ccgtatcgca cggcgttctt
     1081 ccatgattcc aacctgcccg acggcttcaa cgtcatgcgc ctgccgtcgc gccaccatgt
     1141 gttcgagttc acgctggccg agccgagcgg caagcagccg gtcgccaccg acatccagct
     1201 ggtctgcccg ccgatggccg agcgcggctg gcgcggcagt tcccggcacg gcgcaagcag
     1261 cagcgccgct gccgcggcga gtacggcggc cgaggccgag ggctctgccg ggcacgacga
     1321 tgaccaggac gccgacgacg gcgatcatcc gaacggaaac gttgcatgag agcagcccgc
     1381 atggccccgt gccgactgag cctggcagcc gcgctgctgc tgtcgtttgc caccgtcgcc
     1441 tgcgccgcca ccgtcggcga cgccctgccg cccctgccga agaccctgcc ggacctgtcg
     1501 cccgataccg tgcagtcgag cgtcgacaag acggtcgcgg gcctgctgac gcagtaccac
     1561 taccggcggg tcaaaatcga cagcgcgctg tcggcgacgg tgttcgacga ttacctgtcg
     1621 gcgctcgatt tcagccacag cttcctgctc gccagcgatg tcgccgaact cggccagtat
     1681 cgcgacaaga tcgatgacga actgcgcaac ggccggctcg atgctgcctt cgcgatctac
     1741 aacctctatc agcagcggct gctggagcgc acgagacgca tcagcgagga gctgacgcgg
     1801 ccgttcgact tcaggaagga cgaaacctac gaggccgacc gcaagaaggc accgtgggcg
     1861 cgcacggcgg ccgaactcga cgagatctgg cgcaagcgcc tgaagaacga gacgctgagc
     1921 ctgatgctcg ccggcaagga cgaggctgcc gcccgcgaca cgctgcgcaa gcgctacgaa
     1981 ggccggctgc gccgcgccgc gcagtccggc cccgaggacg tgttccagat ctacatgaac
     2041 gccgtcaccg gcgccttcga tccgcacacc tcgtacctga gtccgcgcaa ttccgagaac
     2101 ttcaacatcc agatgcgcct gtcgctggag ggcatcggcg cggtgctgcg gcttgaggac
     2161 gagcagaccg tggtcgtcga gctggtgccc ggcggtccgg ccgacctgtc caagcagatc
     2221 cggcccaagg accgcatcct cgcggtcggc cagggcgatg acggcgcgat gaccgacgtg
     2281 gtcggctggc ggctcgatga tgtcgtcgac ctgatccgcg gccagcgcgg ctcggtcgtg
     2341 cgcctgcagg tggtgccggc gcaggccgcg gccggcagtc cgggcacggt cgtgcgcatc
     2401 gtgcgcaaca agatccagct ggaggagcag gcggccaaga gtcaggtcaa gaccttcgtg
     2461 cgcgatgggg tcgagcgcaa ggtcggcgtc atcacgatcc cgaccttcta catcgacttc
     2521 gcggccgcgc agcgcggtga cctcgactat cgcagcacga cgcgcgacgt gcgccggctg
     2581 atcgagcagc tgcagcgcga gcgcgtcgag ggcatcgtca tcgacctgcg ccagaacggc
     2641 ggcggctcgc tgcaggaagc cgtcgagctg accgggctgt tcattgcgga cgggccggtc
     2701 gtgcaggtgc gcgatgccaa gggcaaggta caggtcgaga acgatcccga tccgaagcag
     2761 ttctattcgg ggcatctcgc ggtgctgacc gaccgcttca gtgcctcggc ctcggaaatc
     2821 ttcgccgcgg cgatccagga ctacggccgc ggcgtgctcg tcggcgagca gaccttcggc
     2881 aagggcacgg tgcagacgct gatcgatctc ggccgcttcc tgccgcaggt caaggacaag
     2941 ctcggccagc tgaagctgac catcgccaag ttctaccgca tcaacggcga gagcacgcag
     3001 aaccgcggcg tgctgcccga tgtcgcgctg ccgccggcct tcgcgaccga cgaggtcggc
     3061 gagagcgccc aggagcacgc cctgccctgg gacgaaatac cgccgacccg ctacaaggtg
     3121 gccggccggg tgcgcgacgg cgtcactgaa ctgaagcgcc tgcacgaggc gcgcgtgccg
     3181 cacgatcagc cctaccagga cctgaagcac gacctggagg tgtcgatggc gctgcgcaag
     3241 cagaccacgg tatcgctgct ggagagcaag cgtcgcaccg atcaggaagc cagcaagagc
     3301 cgcatgcgca ccgcgcgcgg ccgggtcatc gatccggaca gcgacgatgc cggcgacgcc
     3361 aagaaggacg gacccgatcc cttcctcgac gagaccgggc agatcctgct cgactggatc
     3421 gacctgcgcg ccggtcgcat cgcccaggcg ccggccgact gacgggcgca tcggcgcgct
     3481 gcagacccgc gggggcgtca tggtgacgcc cccgcagcat tctgacggga catgaacgtt
     3541 ccgtcataca cagagcggtc aaaccgccgc aacatatggt ccccaggatg gcgcaccgtg
     3601 gaatcgcttc cgcgctgcga gatccggaca ggaggagacc caccatgaac gaggagatgc
     3661 gcgaaatcgt ccgccgggcg gaactggaac tggggcgcga actcaccgag cgtgaattcg
     3721 cccgttacgt cggcaaccgc ctgtcggtgc cgttcacgga
//
LOCUS       DAOQJH010000213         3756 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_15212_length_3756_cov_3.827614, whole genome shotgun sequence.
ACCESSION   DAOQJH010000213 DAOQJH010000000
VERSION     DAOQJH010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3756)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3756)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3756
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15212_length_3756_cov_3.827614"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..505)
                     /locus_tag="PLQ18_06030"
     CDS             complement(<1..505)
                     /locus_tag="PLQ18_06030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE01246.1"
                     /translation="MLIFSDYGQPGLGELGYGIATLMYLVFAAALATQWRGRLRGAVL
                     VAAVAITAGWTLSAALVAAGVRAALPFYLALETLHAAAWLYFLIVLLRPLILAQQHRH
                     VALAIARPATGALAAALLLVDLLPAVADWLPESLLRDLRIFGHLAMAVGGLVLIEQLF
                     RNTRPEQR"
     gene            complement(524..1918)
                     /locus_tag="PLQ18_06035"
     CDS             complement(524..1918)
                     /locus_tag="PLQ18_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018509072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03013 family PEP-CTERM/XrtA system
                     glycosyltransferase"
                     /protein_id="HNE01247.1"
                     /translation="MIRLFRHYVPLPLLLLALVEMLVFFGSIPFGKFVRSGIEGWAFD
                     PIGLELKASLFAIVMVSVMTAMGLYERHFWDGKADMMLRVAVGFLLGLFTITLIYYFL
                     PDLYMGRGEFGIAFAASFVCVVVARYAFFRLSDHEALKRRVLVLGTGYKAHQVEQLAS
                     GSHGRGFALIGFVRMNEADDIIVDAARVLPAGTLLRRYTEHERIDEIVVAMDDRRRGF
                     PVDDILDCKINGVTVSDLATFFERESGKIRLDALHPSSMIFSDGFHQAALKSFSKRCF
                     DIIASLLLLGLTWPVMLATAIAIWWESNRNGPIFYKQARVGRNDRVFDVLKFRSMRTD
                     AEQNGIAQWAQKNDSRITRVGAFIRKTRIDELPQLFNVLRGEMSFVGPRPERPQFVSM
                     LEQTIPYYSMRHRVNPGITGWAQICYPYGASEEDAREKLQYDLYYIKNYSLFLDFMVL
                     LQTAHVILWGKGAR"
     gene            complement(2064..2777)
                     /gene="dnaQ"
                     /locus_tag="PLQ18_06040"
     CDS             complement(2064..2777)
                     /gene="dnaQ"
                     /locus_tag="PLQ18_06040"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012969622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HNE01248.1"
                     /translation="MRQIVLDTETTGLEPAQGHRIIEIGCVELIGRRPTGRSFHRYLN
                     PERALDPGAVEVHGLTEAFLADKPRFAEVADELLEFIRGAELVIHNAAFDTGFLDAEL
                     ARLKPAPGRIADFCPVLDTLKLARELHPGARASLDALCRRYGIDNSQRELHGALLDAR
                     ILADVYLAMTGGQVAMALDGADAPASAAARSAEPEAVVRIAAGRPALRVIRATAADLE
                     ADERLQALFGHRFEGGPAT"
     gene            complement(2833..3309)
                     /gene="rnhA"
                     /locus_tag="PLQ18_06045"
     CDS             complement(2833..3309)
                     /gene="rnhA"
                     /locus_tag="PLQ18_06045"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009738673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HNE01249.1"
                     /translation="MNAPTTADADLVVIHTDGACSGNPGPGGWAAILSWRGRSRELSG
                     ASAATTNNIMELTAAIMALEALKRPSRVRLHTDSRYVMDGITRWIHGWKKNGWKTSAR
                     EPVKNAGLWQRLDAACAPHTIEWCWVRGHAGDPGNERADQLARAAIGTLRSPPPAG"
     gene            complement(3306..>3756)
                     /locus_tag="PLQ18_06050"
     CDS             complement(3306..>3756)
                     /locus_tag="PLQ18_06050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE01250.1"
                     /translation="VPHLHEFVARPEELFDGIGTALIPDGHVVVLGFNPLLDGGIWRL
                     LRVREARLPAGARLYGSAVVRGWLRRAGFETDAVRFHCRWPWDGALRLRGWPAALGAC
                     GYLIVGRKRVSTLTPVRPRWRPRRRLVPAGAVGDASRVGQCCEDRIR"
BASE COUNT          615 a         1302 c         1319 g          520 t
ORIGIN      
        1 agcgctgctc gggccgggtg ttgcggaaca gctgctcgat cagcaccagt ccgccgaccg
       61 ccatcgccag atgcccgaag atgcgcaggt cgcgcagcag cgattccggc agccagtccg
      121 cgaccgccgg cagcaggtcg accagcagca gcgcggcggc cagcgcgccg gtcgccggtc
      181 gcgcgatcgc gagcgccaca tggcgatgct gctgggcgag gatcagcggc cgcagcagca
      241 cgatcaggaa gtacagccac gccgccgcgt gcagggtttc gagggcgaga tagaacggca
      301 gcgccgcgcg cacgccggcg gcgaccagcg ccgccgacag cgtccagccg gcggtgatcg
      361 cgacggccgc aaccagcacc gccccgcgca gccggccgcg ccactgcgtg gccagcgccg
      421 cggcaaacac caggtacatc agcgtcgcaa tgccgtagcc gagctcgccg agcccgggct
      481 ggccgtagtc actgaagatc agcatgtgcc cgcctccgcc ggatcagcgg gcccccttgc
      541 cccacaggat cacgtgggcc gtctgcagca gcaccatgaa atcgaggaac aggctgtagt
      601 tcttgatgta gtagaggtcg tactgcagct tctcgcgggc atcctcctcg gaggccccgt
      661 acggatagca gatctgcgcc cagccggtga tgccggggtt cacgcggtgg cgcatcgagt
      721 agtacgggat cgtctgctcc agcatcgaca cgaactgcgg ccgctcgggc cgcgggccga
      781 cgaagctcat ctcgccgcgc agcacgttga acagctgcgg cagctcgtcg atgcgggtct
      841 tgcggatgaa ggcaccgacg cgggtgatgc gcgagtcgtt cttctgcgcc cactgcgcga
      901 tgccgttctg ctcggcatcg gtgcgcatgc tgcggaactt cagcacgtcg aacacgcggt
      961 cgttgcgacc gacccgggcc tgcttgtaga agatcgggcc gttgcggttc gattcccacc
     1021 agatcgcgat ggccgtggca agcatcaccg gccaggtcag gccgagcagc agcaggctgg
     1081 cgatgatgtc gaagcagcgc ttgctgaagg acttcagcgc cgcctgatgg aagccgtccg
     1141 agaagatcat cgaactcgga tgcagcgcat cgaggcggat cttgccgctc tcgcgctcga
     1201 agaaggtcgc cagatcgctg acggtgacgc cgttgatctt gcagtcgagg atgtcatcga
     1261 ccgggaagcc gcggcggcgg tcgtccatgg ccacgacgat ctcgtcgatg cgctcgtgct
     1321 cggtgtagcg gcgcagcagc gtgccggccg gcagcacgcg cgcggcgtcg acgatgatgt
     1381 cgtcggcctc gttcatgcgc acgaagccga tcagcgcgaa accgcggccg tggctgcccg
     1441 aggcgagctg ctcgacctgg tgggccttgt agccggtgcc gagcaccagc acgcggcgct
     1501 tgagcgcctc gtggtccgac agccggaaga acgcatagcg ggcgacgacc acgcagacga
     1561 aggaagccgc gaacgcgatg ccgaactcgc cgcggcccat gtagagatcg ggcaggaagt
     1621 aatagatcag cgtgatcgtg aacaggccga gcaggaagcc gaccgcgacg cgcagcatca
     1681 tgtcggcctt gccgtcccag aagtggcgct cgtacagccc catggccgtc atgaccgaga
     1741 ccatgacgat cgcgaacagc gaggccttca gctccagccc gatcggatcg aaggcccagc
     1801 cctcgatgcc gctgcgcacg aacttgccga acgggatgct cccgaagaag accagcattt
     1861 ccacgagggc cagcagcagc agcggcagcg gcacgtaatg tctgaacagg cgaatcatcc
     1921 gtcatcccca ggggcaagtc cgcttcggcg tccggttccg gggcagccgg tcggtcgcga
     1981 caacggggcc agactctacc atcggcccgc ccgcccccgt ccgaacgagc tgtgtcgccg
     2041 ctgtgtaaag ttgataaccg gcttcaggtc gccggaccgc cctcgaaacg gtggccgaac
     2101 agcgcctgca gccgctcgtc ggcttcgagg tccgcggccg tggcccggat cacgcgcagc
     2161 gccggccgcc cggccgcgat gcgcacgacg gcctcgggct cggcgctgcg ggccgcggcg
     2221 ctggccggcg catcggcacc gtcgagcgcc atcgccacct gaccgccggt catcgccaga
     2281 tagacatcgg cgaggatgcg cgcgtcgagc agcgcgccgt gcagctcgcg ctgggagttg
     2341 tcgatgccgt agcgccggca cagcgcatcg agcgaggcgc gcgcgccggg atgcagctcg
     2401 cgcgcgagct tcagcgtgtc gagcaccggg cagaaatcgg cgatgcgccc gggcgcaggc
     2461 ttcaggcgtg ccagctcggc atcgaggaag cccgtgtcga acgcggcgtt gtggatgacc
     2521 agctcggcgc cgcggatgaa ctccagcagt tcatcggcca cctccgcgaa gcggggcttg
     2581 tcggccagga aggcctcggt caggccatgg acctcgaccg ccccgggatc gagcgcccgc
     2641 tccgggttca ggtagcggtg gaagctgcgc ccggtcggcc ggcggccgat cagctcgacg
     2701 cagccgatct cgatgatgcg gtgaccctgg gccggttcga gtccggtggt ttcggtgtcg
     2761 aggacgatct ggcgcacgac ggaggactcg cgaggtgggg ggaaggagca gggtttcgac
     2821 ggtccggacg gttcagcccg ccggcggcgg actgcgcagc gtgccgatcg cggcgcgggc
     2881 gagctgatcg gcgcgttcgt tgccggggtc gccggcatgg ccgcgcaccc agcaccattc
     2941 gatcgtgtgc ggtgcgcagg cggcatcgag ccgctgccag agtccggcat tcttgaccgg
     3001 ctcgcgggcg ctggtcttcc agccgttctt cttccagccg tgaatccagc gcgtgatgcc
     3061 gtccatcacg tagcggctgt cggtgtgcag gcgcacgcgg ctcgggcgct tcagcgcctc
     3121 cagcgccatg atcgccgcgg tcagctccat gatgttgttg gtcgtcgcgg cactggcacc
     3181 gctcagctcg cggctgcgtc cgcgccagct caggatcgcc gcccagccac ccggccccgg
     3241 gttgccggag caggcaccgt cggtatggat cacgaccaga tccgcatcgg ccgtcgtcgg
     3301 agcgttcacc ggattcgatc ctcgcagcac tggccgacgc gcgaggcatc gccgaccgcc
     3361 ccggccggca ccagccgccg gcgcgggcgc cagcgcgggc gcaccggcgt cagcgtcgag
     3421 acgcgcttgc gcccgacgat caggtaaccg caggcaccga gcgccgccgg ccagccccgc
     3481 agccgcagcg caccgtccca gggccagcgg cagtggaagc gcacggcgtc ggtctcgaag
     3541 ccggcacgcc gcagccagcc gcgcacgacg gcactgccat acaggcgcgc cccggccggc
     3601 agccgcgcct cgcgcacgcg cagcagccgc cagatgccgc cgtcgagcag cggattgaag
     3661 ccgagcacga cgacgtgtcc gtccgggatc agcgccgtgc cgatgccgtc gaacagttct
     3721 tcgggcctgg caacgaattc gtgcaggtgc ggcacg
//
LOCUS       DAOQJH010000214         3754 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_15221_length_3754_cov_3.573669, whole genome shotgun sequence.
ACCESSION   DAOQJH010000214 DAOQJH010000000
VERSION     DAOQJH010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3754)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3754)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3754
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15221_length_3754_cov_3.573669"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..313
                     /locus_tag="PLQ18_06055"
     CDS             <1..313
                     /locus_tag="PLQ18_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007112189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HNE01251.1"
                     /translation="WGPEVRLSARTGAGLDALAAHLKHCMGFADKQEGAFTARRRHLD
                     ALERTRVHLQDARLRLAGRQGELAAEDLRRAHAALGEITGEVSADDLLGAIFGQFCIG
                     K"
     gene            559..1074
                     /locus_tag="PLQ18_06060"
     CDS             559..1074
                     /locus_tag="PLQ18_06060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNE01252.1"
                     /translation="MHRSWFSRLFGGSTRDEAFAPVPATEARKAEAGGFDSAESLRAC
                     LDCLPGNVFFADPAFRLTYMNDAARHTVQAVEPEIRRVFGVSAADILNGSIHRFHRDP
                     ARVEAILQNYVQTLPREATFSFGSVTLRTAINAVLTPDRRLLGYIVNWEDVSRLRSME
                     AEVEERRERAR"
     gene            1120..1578
                     /locus_tag="PLQ18_06065"
     CDS             1120..1578
                     /locus_tag="PLQ18_06065"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNE01253.1"
                     /translation="MQEISHSAAQVVEVSNTGQTTADAASESIRKLGTQSQEIGSVLK
                     LIDTIAAQTRLLALNATIEAARAGDAGKGFAVVANEVKELAAATAGATEEISAKIGAI
                     RSGVTEAVEAIERIQSIMREVHRLQMTVAAAVEEQSVVVGELGRGASEVL"
     gene            1716..2087
                     /locus_tag="PLQ18_06070"
     CDS             1716..2087
                     /locus_tag="PLQ18_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017935738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE01254.1"
                     /translation="MGKRALVIDDSRAMRRVIANILTERGFEVLEAENGQVALDQLEA
                     NPGIEVALVDWNMPVMNGYDFIKAVRAQPKYARLTMMMVTTETEMSQVVRALAAGANE
                     YVMKPFTADALVEKLELLGLL"
     gene            2197..3021
                     /locus_tag="PLQ18_06075"
     CDS             2197..3021
                     /locus_tag="PLQ18_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HNE01255.1"
                     /translation="MTPSSADYDYIRRLVQDHSAIMLEAGKEYLLGLRLQGLMTRHGC
                     ADIPALVQKLRSDRSGALSREVVEAMTNNETSFFRDLHPFETLRKVIVPELIRRRAAE
                     RTLDIWCGASSSGQEIYSVALTLREHFPELVNWRLNIVSTDLSREMVQRGRDGRYSQI
                     EVNRGLPAMLLVKYFERSGMDYYVKPDIRRMVDFRELNLNGPWSGVPSRLDIVFMRNV
                     LIYFEDAVKRRILAKVHDLLRPDGYLFLGGSETTLNLEDRLEQVQLERTRCFRRRH"
     gene            3164..3628
                     /locus_tag="PLQ18_06080"
     CDS             3164..3628
                     /locus_tag="PLQ18_06080"
                     /inference="COORDINATES: protein motif:HMM:NF013487.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNE01256.1"
                     /translation="MQKLPTVLALSTTVLALAVSLSGCEQSDPDSPAFKRRQAFKQIV
                     RAAEPLGKMARGKDPYQPDTFIDRAHELQQVAGLPWDHFPPPQPTDREKTRAKPAIWQ
                     QTEKFETERRNFIAAVNGVVAAGDTRSLDRVRPAVEKLENACMSCHKAFRAD"
BASE COUNT          643 a         1306 c         1248 g          557 t
ORIGIN      
        1 gtgggggccg gaagtccggc tgagcgcgcg taccggggct ggtctcgatg cgctcgccgc
       61 tcacctaaag cattgcatgg gctttgccga taagcaggaa ggtgcgttca cggcccgccg
      121 ccgccacctc gacgccctcg aacggacgcg cgtgcatctg caggatgcgc gcctgcgcct
      181 ggccgggcgt cagggcgagc ttgcggccga ggatctgcgc cgcgcccatg ccgcgctcgg
      241 cgagatcacc ggcgaggtca gcgccgatga cttgctcggc gcgatcttcg ggcaattttg
      301 cataggcaag taaggcttgc caccggaatt gcgcagtgcg gcataacctt gaattactgt
      361 aatttttcct gacacggcat cgacgccggt ttgtgctgca cgcaatcatg gccctccccg
      421 acggcagcgg atgccggggc gctgcaccgg ttcccggaag cgtccgcccg ccgccgccgc
      481 agcggccgcc ggcagtggcg cagtcccttg cagatgcccc gacgatctcc ctgacccccc
      541 gcattccgga gagcgcttat gcaccgcagc tggttttccc gcctgttcgg cggctcgaca
      601 cgtgacgagg cctttgctcc cgtgccggcg acggaggcca ggaaagccga ggccggcggc
      661 ttcgattcgg ccgagagcct gcgcgcctgc ctcgactgcc tgcccggcaa cgtgttcttc
      721 gccgacccgg ccttccggct cacgtacatg aacgacgcgg cgcggcacac cgtgcaggcg
      781 gtcgagcccg agatccggcg cgtgttcggc gtcagcgccg ccgacatcct gaacggctcg
      841 atccaccgct tccaccgaga tccggcgcgc gtcgaggcca tcctgcagaa ctacgtgcag
      901 acgctgccgc gcgaggcgac cttctcgttc ggcagcgtca cgctgcgcac cgcgatcaac
      961 gccgtgctga ccccggaccg gcgcctgctc ggctacatcg tcaactggga ggacgtctcg
     1021 cgcctgcgtt cgatggaggc cgaggtcgag gaacggcgcg agcgcgcccg ctagtccgcc
     1081 gaagacctgc tgcaccggct caacgagctg cgcaccagcg tgcaggagat ctcgcacagc
     1141 gccgcgcagg tggtcgaagt ctcgaacacc ggccagacca ccgccgatgc cgccagtgaa
     1201 tcgatccgca agctcggcac gcagagccag gagatcggca gcgtgctgaa gctgatcgac
     1261 accatcgccg cgcagacgcg gctgctggcg ctgaacgcca cgatcgaggc cgcgcgcgcc
     1321 ggcgatgccg gcaagggctt cgcggtggtc gccaacgagg tcaaggaact ggcggcagcg
     1381 acggccgggg ccaccgagga aatttccgcg aagatcggcg ccatccgcag cggtgtcacc
     1441 gaggcggtcg aggcgatcga gcgcatccag tcgatcatgc gcgaggtgca ccggctgcag
     1501 atgacggtgg ccgcggcggt cgaggaacag agcgtcgtgg tcggcgagct cggccgcggc
     1561 gcgagcgaag tgctgtaagc ccggcgcgac ggccggcggg cgaccggttt caacagattt
     1621 caactgaatt tcagacggcg gcccggccgc cgatggtgca ttcaagaccg gcacgcgaag
     1681 ctgcggatcg ccggaagcat tgggagaatg aggcgatggg caagcgtgca ctggtgatcg
     1741 acgattcgcg ggcgatgcga cgcgtgatcg cgaacatcct gaccgagcgc ggcttcgagg
     1801 tgctggaggc cgagaacggc caggtcgccc tcgaccagct cgaagcgaat cccggcatcg
     1861 aggtcgcgct ggtcgactgg aacatgccgg tcatgaacgg ctacgacttc atcaaggccg
     1921 tgcgtgcgca gccgaagtac gcgcgcctga cgatgatgat ggtcacgacc gaaaccgaga
     1981 tgagccaggt ggtgagggcc ctggccgccg gcgcgaacga atacgtcatg aagccgttca
     2041 cggccgatgc gctggtcgag aagcttgaac tgctcggact gctctgaacc tcgcgggacc
     2101 gcgaccggat cgcacggaca agaccgcagc ccggacgagc gtcaggatcc gtgacgacgc
     2161 gggggcgcac aagaaagctg taccgggagt gaggcgatga caccgagcag tgccgattac
     2221 gactacatcc gccggctggt gcaggaccac tcggccatca tgctggaggc cggcaaggaa
     2281 tacctgctcg gcctgcgcct gcagggcctg atgacccgcc acggctgcgc cgacatcccc
     2341 gcgctggtgc agaagctgcg cagtgaccgc agcggcgcgc tgtcgcgcga ggtcgtcgag
     2401 gcgatgacca acaacgagac ctcgttcttc cgcgacctgc atccgttcga gacgctgcgc
     2461 aaggtcatcg tgcccgagct gatccgccgc cgcgccgccg agcgcacgct cgacatctgg
     2521 tgcggggcct cgtcgagcgg tcaggagatc tactcggtgg cgctgacgct gcgcgagcac
     2581 ttcccggagc tggtgaactg gcggctcaac atcgtctcga ccgacctgtc gcgcgagatg
     2641 gtccagcgcg gccgcgacgg ccgctacagc cagatcgagg tcaaccgcgg cctgccggcg
     2701 atgctgctcg tgaagtactt cgagcgctcg ggcatggatt actacgtcaa gcccgacatc
     2761 cggcgcatgg tcgatttccg cgagctgaac ctcaacggcc cgtggagcgg cgtgccctcg
     2821 cgcctcgaca tcgtgttcat gcgcaacgtg ctgatctact tcgaggatgc cgtcaagcgc
     2881 cgcatcctcg ccaaggttca cgacctgctg cgcccggacg gctacctgtt cctcggcggc
     2941 tccgagacca cgctgaacct cgaagaccgc ctcgaacagg tgcagctcga acgcacgcgc
     3001 tgcttccgcc gccggcactg agccggcacg ggccggtgat acggaaaagc ctcgtttaag
     3061 ccggccatcc accggcatct gctagagtcc gcgccttctc gcgcgggcct ccggcaacag
     3121 cagcacgggt gcccgctccg tcgctgcccc cggatgtccg cccatgcaga agctcccgac
     3181 cgtgctcgcc ctgagcacca ccgtcctcgc gctcgccgtc tcgctttccg gctgcgagca
     3241 gtccgatccg gacagcccgg ccttcaagcg ccgtcaggcc ttcaagcaga tcgtccgggc
     3301 cgccgaaccg ctcggcaaga tggcgcgcgg caaggacccg taccagcccg acaccttcat
     3361 cgaccgtgcg cacgaactgc agcaggtcgc cggcctgccc tgggatcact ttccgccgcc
     3421 gcagcccacc gatcgcgaga agacgcgcgc gaagccggcg atctggcagc agaccgaaaa
     3481 attcgagacc gagcgccgca acttcatcgc cgcggtcaac ggcgtggtcg ccgccggcga
     3541 cacgcgcagc ctcgatcgcg tgcgcccggc ggtcgagaag ctcgaaaacg cctgcatgag
     3601 ctgccacaag gccttccgcg ccgactgagc ctgcagcgct gccgtgctgc gggtgcaaca
     3661 cgccgccttc aagtcgcggg ggggcgtggc tacactgggc gccctttttc cgcccgccgt
     3721 cccgcccgag gcttcgcgct ttgaagacgc aact
//
LOCUS       DAOQJH010000215         3738 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_15315_length_3738_cov_4.320391, whole genome shotgun sequence.
ACCESSION   DAOQJH010000215 DAOQJH010000000
VERSION     DAOQJH010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3738)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3738)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3738
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15315_length_3738_cov_4.320391"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            67..582
                     /gene="coaD"
                     /locus_tag="PLQ18_06085"
     CDS             67..582
                     /gene="coaD"
                     /locus_tag="PLQ18_06085"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018867926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HNE01257.1"
                     /translation="MSVVALYPGTFDPLTNGHQDLVERGARMFDRLIVAVAANPGKGP
                     LFTLEQRVDHARQILGHLHNVEICGFDILLVNFAKHVGANVILRGLRAVSDFEYEFQL
                     ASMNRRLHAAIETVFLTPAEQYSFISSTLVREVAKFGGDISAFVSPVVERALAEKFGL
                     PPKAPLPGSVK"
     gene            611..865
                     /locus_tag="PLQ18_06090"
     CDS             611..865
                     /locus_tag="PLQ18_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017938521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HNE01258.1"
                     /translation="MSLMITDECINCDVCEPECPNGAISQGDEIYVIDPNLCTECVGH
                     FDTPQCRGVCPVDCIPNDPSHVETPEQLQEKYQRLSGLIG"
     gene            966..1472
                     /locus_tag="PLQ18_06095"
     CDS             966..1472
                     /locus_tag="PLQ18_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009146612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HNE01259.1"
                     /translation="MIERCIETLIFGSRWLLVPLYLGLAAVLVLFSARAVIEIVHVFQ
                     AFPDIGEIDLLLGVLGLIDFVLVANLLVMVILSSYEAFVSRLDVADAQERPGWLGKVD
                     AGTVKIKLAAAIVAVSSIHLLKVFMKIDRYDDRQLWWLCGIHLVFVVSALLMAVVDKL
                     AFAAHREH"
     gene            1613..2467
                     /locus_tag="PLQ18_06100"
     CDS             1613..2467
                     /locus_tag="PLQ18_06100"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNE01260.1"
                     /translation="MTIDSAAGSPVPPCVLVLGRDLAAARAYAARLVHAGYRAVAGVP
                     ALDSAPVAALLLGGEGESEGTAWAEAVAPLLGDLPSRIEIGPLPEDFAGLAHWLDGLG
                     APVQERRVMSTGLVLSEVADGEAAAGGLLAAADFERRLGVELARCQRYGRVLSFGLLD
                     PDGLSALRSRSPAAADRVLRQLARFTVAHLRRTDVSGRCGAGGIGVLLTETTAARGLG
                     VFERLRVGFVQGQLRVQPREPAVSLSIGVVECRGERDAAGVLAVAQTAGEAARAAGGD
                     CVRLAPAA"
     gene            complement(2480..3544)
                     /locus_tag="PLQ18_06105"
     CDS             complement(2480..3544)
                     /locus_tag="PLQ18_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002707078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNE01261.1"
                     /translation="MNRLPTLARLGALLLALLSSLACAPARAADERTLVLYNWAEYMP
                     RPVLKQFTKETGIKVRYSTYDNNEAMFAKLKAVEASGGYDLVVPSSYYVTRMRAAGML
                     RPLDKDRLPNLKNLDPRLLDKPYDPGNAYSVPYLWGASGIAVDSARIDPTTVTSWADL
                     WKPEYRGRLMLLDDLREVFGIALKLDGRSINSRDPDEIRAAYEKLLELRPNVKVFNAE
                     SPKSVLLSGEVRIGQIYNGEAWMAEQANPKIRFVIPKEGPLLWVDNLVIPKSARHVEE
                     AHAFIDFVLRPEIAKQIAEEVGYTTPNLAARALLAKKVRDSRTAYPSDADLERGEFQT
                     DVGDALPLYEQYWSKLKAAR"
BASE COUNT          528 a         1257 c         1311 g          642 t
ORIGIN      
        1 tgtgaggccg ggagcggcaa cgcataatcc gtcgcccttt tccgaccagt gccgacaggt
       61 atccgcatga gcgtcgttgc cctgtacccg ggtacgttcg atccgctgac caatggtcat
      121 caggatctcg tcgagcgcgg cgcccgcatg ttcgaccggc tgatcgtcgc ggtggcggcc
      181 aatcccggca agggtccgct gttcacgctc gaacagcgcg tcgatcacgc ccgccagatt
      241 ctcggtcacc tgcacaacgt cgagatctgc ggcttcgaca tcctgctggt caatttcgcc
      301 aagcacgtcg gcgccaacgt catcctgcgc ggcctgcgcg cggtgtcgga tttcgaatac
      361 gaattccagc tcgccagcat gaaccgccgc ctgcacgcgg cgatcgagac cgtgttcctg
      421 acgccggccg agcagtactc gttcatttcc tcgacgctgg tgcgcgaggt cgcgaagttc
      481 ggcggcgaca tctccgcctt cgtcagtccg gtggtcgagc gtgcgctggc cgagaagttc
      541 ggactgccgc cgaaggcacc gctgcccggt tccgtgaagt aacggcccgt ccgggccgga
      601 ggtccatgcc atgtccctga tgattaccga cgaatgcatc aactgcgatg tctgcgagcc
      661 ggagtgcccg aacggcgcaa tctcgcaggg tgacgagatc tacgtcatcg acccgaacct
      721 gtgtaccgaa tgcgtcggcc atttcgatac gccgcagtgt cgcggtgtct gtccggtcga
      781 ctgcatcccg aatgacccga gccacgtcga aacgcccgag cagctgcagg aaaagtatca
      841 gcgcctgtcc ggcctgatcg gctgacgagc cgcggccgga accatcgggc cccgctcgcc
      901 ggggcctttt ttatttccct gaccggacac ctgccaggca gggtccggcg caagccccgg
      961 gcatcatgat cgaacgctgc attgaaaccc tgatcttcgg cagccgctgg ctgctcgttc
     1021 cgctgtacct cggtctggcc gcggtgctgg tgctgttctc ggcccgggcc gtgatcgaga
     1081 tcgtgcacgt cttccaggca tttcccgaca tcggagagat cgacctgctg ctcggcgtgc
     1141 tcgggctgat cgacttcgtg ctggtcgcca acctgctggt catggtgatc ctgtcgagct
     1201 acgaggcctt cgtctcgcgc ctcgatgtcg ccgacgctca ggagcgtccg ggctggctcg
     1261 gcaaggtcga tgccggcacc gtcaagatca agctcgcggc cgcgatcgtc gcggtgtcgt
     1321 cgatccacct gctgaaggtg ttcatgaaga tcgaccgcta cgacgaccgc cagctctggt
     1381 ggctgtgcgg catccacctg gtgttcgtgg tctcggcgct gctgatggcc gtggtcgaca
     1441 agctggcctt cgccgcgcac cgcgagcact gatccggctt cattcgggca cgccggttca
     1501 aggccgccgg cgggcagccg acagtattgc ttcggacccg ggcaccatgg ctcgtggcat
     1561 gcgcaatact cgtcggtgat ccagagattg ccgaagagcc ggaggccagg tgatgaccat
     1621 cgattcagca gcgggaagcc cggtgccgcc ctgcgtactg gtgctggggc gcgatctcgc
     1681 cgccgcccgc gcctacgccg cgcggctggt gcatgccggt taccgggccg tcgccggggt
     1741 gccggcgctc gattcggcgc cggtcgcggc gctgctgctc ggcggcgaag gcgaaagcga
     1801 aggcacggcc tgggccgaag cggtggcgcc gctgctcggt gacctgccga gccgcatcga
     1861 gatcggtccg ctgcccgagg atttcgccgg cctcgcgcac tggctcgacg ggctcggcgc
     1921 gccggtgcag gaacgccggg tgatgagcac ggggctggtg ctcagcgagg tcgccgacgg
     1981 cgaggccgcg gccggcggcc tgctggcggc cgccgacttc gagcggcgcc tcggcgtcga
     2041 actggcgcgc tgccagcgct acgggcgggt gctgagcttc gggctgctcg atccggacgg
     2101 cctgtcggcg ctgcgcagcc gcagccctgc cgccgccgac cgcgtcctgc gccagcttgc
     2161 gcgcttcacg gtcgcgcacc tgcgccgcac cgatgttagc ggccgctgcg gcgccggcgg
     2221 catcggcgtg ctgctgaccg aaaccacggc tgcgcgcggc ctcggcgtgt tcgaacggct
     2281 gcgcgtcggt ttcgtgcagg ggcagctgcg cgtgcagccg cgcgagccgg ccgtgagcct
     2341 cagcatcggc gtcgtcgaat gccgcggcga gcgcgatgcc gccggcgtgc tggcggtcgc
     2401 gcagacggcg ggcgaggccg cgcgggccgc cggcggggac tgcgtgcgct tggccccggc
     2461 ggcctgaggc ggcgcggcct cagcgcgcgg ccttcagctt cgaccagtac tgctcgtaca
     2521 gcggcagcgc gtcgccgaca tcggtctgga actcgccgcg ttcgagatcg gcatcggacg
     2581 gataggccgt gcggctgtcg cggaccttct tcgcgagcag cgcgcgcgcg gcgaggttcg
     2641 gcgtcgtgta gccgacctcc tcggcgatct gcttcgcgat ctcggggcgc agcacgaagt
     2701 cgatgaaggc gtgggcttcc tcgacgtggc gcgcgctctt cgggatcacg aggttgtcga
     2761 cccacagcag cgggccttcc ttcgggatca cgaagcggat cttcggattg gcctgctcgg
     2821 ccatccaggc ctcgccgttg tagatctggc cgatgcgcac ctcgccggac agcagcaccg
     2881 acttcggcga ctcggcgttg aacaccttca cgttcggacg cagttcgagc agcttttcat
     2941 aggcggcgcg gatttcgtcg ggatcgcggc tgttgatcga acggccgtcg agcttcagcg
     3001 cgatgccgaa cacctcgcgc aggtcatcga gcagcatcag ccggccgcgg tactcgggct
     3061 tccacagatc ggcccagctc gtgaccgtcg tcgggtcgat gcgcgcgctg tcgacggcga
     3121 tgccgctcgc gccccacaga tacgggacgc tgtaggcgtt gccggggtcg tagggcttgt
     3181 cgagcaggcg cgggtcgagg ttcttcaggt tcggcagccg gtccttgtcg agcggccgca
     3241 gcatgccggc ggcgcgcatg cgcgtgacgt aatagctcga cggcacgacc aggtcgtagc
     3301 cgccggaggc ctcgacggcc ttgagcttcg cgaacatcgc ctcgttgttg tcgtaggtcg
     3361 aatatctgac cttgatgccg gtttccttcg tgaactgctt cagcaccggg cgcggcatgt
     3421 actcggccca gttgtacagc accagcgtgc gctcgtcggc ggcgcgggcc ggggcacagg
     3481 cgagactcga cagcagggcg agcagcagcg ccccgaggcg cgctagggtc ggcagacggt
     3541 tcatcggcgg gcctcttggg cagcaggggg acacgcatgc gccggtcgcg ggcggtagcc
     3601 gcaaccttgg gcactgccgg cgaggcccgc aggcccgaaa gccgcacggg ccgcagaggc
     3661 gtgaccgcag gcgcacgcgc cgtcagtgtt gccgctgccg gcgggcttgc aagaaaaaac
     3721 cccgccggtg cggcgggg
//
LOCUS       DAOQJH010000216         3725 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_15411_length_3725_cov_4.519074, whole genome shotgun sequence.
ACCESSION   DAOQJH010000216 DAOQJH010000000
VERSION     DAOQJH010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3725)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3725)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3725
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15411_length_3725_cov_4.519074"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3364
                     /locus_tag="PLQ18_06110"
     CDS             <1..3364
                     /locus_tag="PLQ18_06110"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE01262.1"
                     /translation="FATRHAGIWAQMANAPAGQVEDAEGLWTWRRISLASTAGKMLSG
                     TAPEFIALTQVPAARLAQQRHEVWLAVGSITAALLLVHSLLSLALAFALAGRARAETG
                     AALAREQALAAVRLHAAQTRYRSVVEGNINGLLVVGPDGRIDLVNPALARMFGYTVED
                     LRGRSIDELLPAAMRTRHVAEREHYMQVPSGRTMGSGRDLYAQRRDGSVFPVEVSLSP
                     FFEDGVTWVQAIVSDITERKHSEALVRKMRDRLQLATDAAGIGVWEWDVVSNTLLWDD
                     WMFRLYHLPEHERIAVLPYQIWHDRCHPGDLERLEALLAAAVAGTDSFDTEFRIVLPS
                     GELRWIRAASVVERDAAGRALRLVGVNQDVTAQRVLETRLLDARDAAEAASRAKSDFL
                     ANMSHEIRTPMNAILGLCQLLLDGELNRRERDYLGKIYTSSRALLGILNDILDYSKIE
                     AGALTLEVTDFNLDELLDTAADLFSVRTDEKGVELVFDVAPTVPELLYGDPLRLGQVI
                     NNLIGNAVKFTERGIIHVRVDIASQGDNHLELRIAVRDTGIGMSGEQCTKLFEAFSQA
                     DSSTSRKYGGTGLGLAICRRLVALMGGDIRVDSTPGEGSTFTFTVRLGYGRHALRHRD
                     PSSLRGTRALVVDDQETSRLVLERILESWAFEVTLAASAAEGLAALDAAAAAGRPFGL
                     LLVDWQMPEFDGVAMAREVRRHMASGMLAPAPIVVMVTAYGREHVLGAVGDGVLDAVL
                     DKPVIPSHLFDTVIGLQHGSTSARQRRVPSGRALQQMTRTIRGARVLLVEDFPTNRLV
                     AQEFLSRMGLIADVAENGREAVDKVATRTYDVVLMDLHMPGMDGLEATRRIRATERGR
                     TLPILAMTAAALAQDREATRAAGMNGHIAKPIDAEELATALLGCIAPRTTAEAGPPPP
                     QTATPPFAIDGLDLTAAVTLSDHDWGFLRRVLQRFQRDFSDAPQRLQAALAAGRHDEA
                     ERLVHSIAGLAPGVGSPALGRQGRSLEAALHGGAPIEPMAFLDELQQVLTALALGLKD
                     VPAPAPAAVADPARVAGLATQLRGLLQEFAIVPLELQQALDAALAGYTTPALRTALLR
                     QIDQLDYDAAEATLDEILRAGRCDLRAADTTP"
     gene            3395..>3725
                     /locus_tag="PLQ18_06115"
     CDS             3395..>3725
                     /locus_tag="PLQ18_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE01263.1"
                     /translation="MSPITHLARPAYVLVVDDTPANITLLAEALSPQYRVRFATSGPR
                     ALELIARNDELPDLILLDVMMPEMDGYEVCRQLKEDAATRDIPVIFVSARNEVSSQEH
                     GFNVGGVD"
BASE COUNT          560 a         1325 c         1316 g          524 t
ORIGIN      
        1 cttcgcgacc cgccacgccg gcatctgggc gcagatggcg aatgcaccgg ccgggcaggt
       61 cgaggatgcg gaaggcctgt ggacctggcg gcggatcagt ctcgccagca cggcgggcaa
      121 gatgctgagc ggcacggccc cggagttcat cgcactgacg caggttccgg cggcacggct
      181 ggcgcagcag cgtcacgagg tctggctggc ggtcggcagc atcaccgccg cgctgctgct
      241 ggtgcattcg ctgctgtcgc tcgcactcgc ctttgcactg gccgggcggg cgcgcgccga
      301 aaccggcgcg gcgctggcgc gcgagcaggc gctcgccgcc gtgcgcctgc atgcggcgca
      361 gacgcgctac cgctcggtgg tcgagggcaa catcaacggc ctgctggtgg tcgggccgga
      421 cgggcgcatc gatctggtca atccggcgct ggcgcgcatg ttcggctaca cggtcgagga
      481 tctgcgcggg cgcagcatcg acgaactgct gccggcggcc atgcggacac gccacgtcgc
      541 ggagcgcgag cactacatgc aggtgccgag cgggcgcacg atgggcagcg gccgcgacct
      601 gtacgcgcag cggcgcgacg gcagcgtgtt cccggtcgag gtcagtctga gcccgttctt
      661 cgaggacggc gtgacctggg tgcaggccat cgtcagcgac atcaccgagc gcaagcactc
      721 cgaggcactg gtgcgcaaga tgcgcgaccg gctgcagctg gccaccgatg ccgccggcat
      781 cggtgtctgg gagtgggatg tcgtcagcaa cacgctgctg tgggacgact ggatgttccg
      841 gctgtaccac ctgccggagc acgagcgcat cgcagtgctg ccctaccaga tctggcacga
      901 ccgctgtcat cccggggacc tcgaaaggct ggaggctctg ctcgcggcag cggtggccgg
      961 aaccgacagc ttcgacaccg agttccgcat cgtgctgccc tcgggcgagc tgcgctggat
     1021 tcgcgcggcc tcggtcgtcg agcgcgatgc cgcaggccgg gcgctgcgcc tggtcggcgt
     1081 caatcaggac gtcaccgccc agcgcgtgct cgaaacccgg ctgctcgatg cgcgcgatgc
     1141 cgccgaggcg gcgagccggg ccaagtccga cttcctcgcc aacatgagcc acgagatccg
     1201 cacgccgatg aacgcgatcc tcggcctctg ccagctgctg ctcgacggcg agctgaaccg
     1261 ccgcgagcgc gactacctcg gcaagatcta cacctcctca agggcgctgc tcggcatcct
     1321 caacgacatc ctcgactatt cgaagatcga ggccggggcg ctgacgctgg aagtcaccga
     1381 cttcaacctc gacgagctgc tcgacaccgc tgccgacctg ttctcggtgc gcaccgacga
     1441 gaagggcgtc gagctggtgt tcgacgtggc accgaccgta cccgagctgc tgtacggcga
     1501 tccgctgcgc ctcggccagg tcatcaacaa cctgatcggc aacgccgtga agttcaccga
     1561 gcgcggcatc atccacgtgc gggtcgacat cgccagccag ggcgacaacc acctggagct
     1621 gcgcatcgcc gtgcgcgaca ccggcatcgg catgagcggc gagcagtgca cgaagctgtt
     1681 cgaagccttc tcgcaggccg acagctcgac ctcgcgcaag tacggcggca ccggcctcgg
     1741 actggcgatc tgccggcggc tggtggcgct gatgggcggc gacatccgcg tcgacagcac
     1801 gccgggcgag ggcagcacct tcacgttcac ggtgcgcctc ggctacggcc ggcacgccct
     1861 gcgccaccgc gacccgtcca gcctgcgcgg cacgcgggcg ctggtggtcg atgatcagga
     1921 aacctcgcgg ctggtgctgg agcgcatcct cgaatcctgg gccttcgagg tgacgctggc
     1981 cgccagcgcc gccgaaggtc tggccgcgct cgatgccgcg gccgctgccg gccggccgtt
     2041 cgggctgctg ctggtcgact ggcagatgcc ggagttcgac ggcgtggcga tggcgcgcga
     2101 ggtgcgcagg cacatggcca gcggcatgct ggctccggcc ccgatcgtgg tcatggtcac
     2161 cgcctacggc cgcgagcacg tgctcggcgc cgtcggcgac ggcgtgctcg atgccgtgct
     2221 cgacaagccg gtgatcccct cgcacctgtt cgataccgtc atcggcctgc agcacggcag
     2281 cacgtcggca aggcagcgcc gcgtgccttc cggccgggcg ctgcagcaga tgacgcgcac
     2341 gatccgcggc gcccgcgtgc tgctggtcga ggacttcccg accaaccggc tggtggcgca
     2401 ggagtttctg tcgcgcatgg ggctgattgc cgatgtcgcc gaaaacggcc gggaagccgt
     2461 cgacaaggtc gcgacccgga cctatgacgt ggtgctgatg gacctgcaca tgcccggcat
     2521 ggacggcctc gaagccacgc gccgcatccg cgccaccgag cgcgggcgca cgctgccgat
     2581 cctcgcgatg accgccgcgg cgctggcgca ggatcgcgag gcgacgcgcg ccgccggcat
     2641 gaacggccac atcgccaagc cgatcgatgc cgaggaactg gccacggcgc tgctcggctg
     2701 catcgccccg cgcaccacgg ccgaagccgg gccgccgccg ccccagaccg caacgccgcc
     2761 gtttgcgatc gacggcctgg acctgacggc ggccgtcacg ctgagcgatc acgactgggg
     2821 cttcctgcgc cgcgtgctgc agcgcttcca gcgggatttc agcgacgcgc cgcagcgcct
     2881 gcaggcggcg ctggccgcag gccggcacga cgaggccgag cggctggtgc acagcatcgc
     2941 cgggctggcg ccgggcgtcg gcagtccggc gctgggccgc cagggccgga gcctggaagc
     3001 cgccctgcac ggcggcgcgc cgatcgaacc gatggcgttt ctcgatgagc tgcagcaggt
     3061 gctgacggca ctggcgctgg ggctgaagga cgtgccggcc ccggccccgg ccgcggtggc
     3121 cgatcccgcc cgggtggcgg gccttgccac gcagctgcgc ggcctgctgc aggagttcgc
     3181 gatcgtgccg ctggagctgc agcaggcgct cgatgcggcg ctggccggct acacgacgcc
     3241 ggcgctgcgc accgcgctgc tgcgccagat cgaccagctc gactacgacg cggccgaggc
     3301 cacgctcgat gaaatcctgc gtgccggccg ctgcgacctg cgggccgccg atacgacccc
     3361 ctgattccga tttccgaacc gacgcgtacc cgagatgagc cccatcaccc acctcgcccg
     3421 accggcctac gtgctggtcg tcgatgacac cccggccaac atcacgctgc tcgccgaggc
     3481 gctgagcccg cagtaccgcg tgcgcttcgc aaccagcggt ccgcgggcgc tggagctgat
     3541 cgcccgcaac gacgagctgc cggacctgat cctgctcgac gtgatgatgc cggagatgga
     3601 cggctacgaa gtctgccgcc agctcaagga agacgcggcg acccgcgaca ttccggtgat
     3661 cttcgtctcg gcgcgcaacg aggtgtcgag ccaggagcac ggtttcaacg tcggcggcgt
     3721 cgact
//
LOCUS       DAOQJH010000217         3718 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_15467_length_3718_cov_4.028392, whole genome shotgun sequence.
ACCESSION   DAOQJH010000217 DAOQJH010000000
VERSION     DAOQJH010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3718)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3718)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3718
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15467_length_3718_cov_4.028392"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..288)
                     /locus_tag="PLQ18_06120"
     CDS             complement(<1..288)
                     /locus_tag="PLQ18_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin"
                     /protein_id="HNE01264.1"
                     /translation="MEQPDSGNLFAGIPARLDAEAFGLLYRSPGVRIERILSRGQSTP
                     PGAWYDQPGDEWVALLAGHAELEFADGRRRALLPGDWLLLPAHCRHRVAATS"
     gene            414..1373
                     /gene="corA"
                     /locus_tag="PLQ18_06125"
     CDS             414..1373
                     /gene="corA"
                     /locus_tag="PLQ18_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005364138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HNE01265.1"
                     /translation="MLIAFLIRDGRLEQTPLHVQDDAPIPPDLMQQIVWVDLNEPTEE
                     EREWVEAQFGLELPEYREMNDLEPSARLYEDEDGLHLHSYFLSDQEEQPRNITVAFAV
                     LGGRLFTLHEDDLSTFRLFRLRARRQIGLVKDVGDIILGLFEAKVEHIADKLEEIYSE
                     LEALSHRVLAETDSDMEDVLSGLAVQEDMNGKARLILLDTRRTLSFLLRYAIAGRGED
                     IREILRDVESLTSHTAFLFDKVNFLMDAAMGFINIEQNKIIKIFSIAAVVFLPPTLVA
                     SLYGMNFQHMPELEWEYGYPMAICLMILAGVAPYWFFKRKGWL"
     gene            complement(1398..1670)
                     /locus_tag="PLQ18_06130"
     CDS             complement(1398..1670)
                     /locus_tag="PLQ18_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011507637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HNE01266.1"
                     /translation="MHDPAHPGELLRDYLPAGMSVSEAARRLGVSRQRLSALLDGRAA
                     INAEMALRLECATGQAAGFWLRMQGQRDLWLARCSARVEGVQALDG"
     gene            complement(1677..2057)
                     /locus_tag="PLQ18_06135"
     CDS             complement(1677..2057)
                     /locus_tag="PLQ18_06135"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HNE01267.1"
                     /translation="MADDGLSFGLSAEILEWLRTVFARYPGIERVLVFGSRAKGTYRN
                     ASDIDLAVLAPEMDDETFAQLWNDLDDLPLVFTLDVLHWDRLPAGRIRDHVIRDGRQV
                     YPRIEPASEEIDAQIIAVDGDSGG"
     gene            complement(2039..2437)
                     /locus_tag="PLQ18_06140"
     CDS             complement(2039..2437)
                     /locus_tag="PLQ18_06140"
                     /inference="COORDINATES: protein motif:HMM:NF020360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HI0074 family nucleotidyltransferase
                     substrate-binding subunit"
                     /protein_id="HNE01268.1"
                     /translation="MDQQRLQERIAAFLKALGQLERAARQPKDEFLRDSVIQRFEFTH
                     ELAWRMLKLRLEAELVLARTPKETLQEALKAGFIDDGSDWSVLQRMRNLTSHTYDESL
                     AETVYAFAVGEGLELYRRLAVQARAWQMTD"
     gene            complement(2523..>3718)
                     /locus_tag="PLQ18_06145"
     CDS             complement(2523..>3718)
                     /locus_tag="PLQ18_06145"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006474488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HNE01269.1"
                     /translation="IVATHADRKRLFVEAGHLIVDLARRYYEQDDARVLPREIASFKA
                     FENAMTLDIAMGGSTNTVLHLLAAAKEGGVQFTMSDIDRLSRRVPNLCKVAPATQKYH
                     IEDVHRAGGIMAILGELDRAGLLHHDVKTVHSDTLAAALAANDIAVATCTDAARKRYL
                     AGPAGIPTQEAFSQDCRWPTADADRKNGCIRDRAHAYSQDGGLAVLFGNLAPDGCIVK
                     TAGVDESILKFTGPARIMESQEDAVAAILNDEIRAGDVVLIRYEGPKGGPGMQEMLYP
                     TSYLKSRGLGKACALITDGRFSGGTSGLSIGHVSPEAAEGGNIALVEEGDPIEIDIPK
                     RSIRIVVSDKDLSERRSRMTASGARAWKPVSRERVVSTALKAYAAMTTSASRGAVRDL
                     SQLER"
BASE COUNT          581 a         1286 c         1206 g          645 t
ORIGIN      
        1 gctggtcgcc gccacccggt gccggcagtg cgccggcagc agcagccagt cgccgggcag
       61 cagcgcccgg cgcctgccat cggcaaactc cagttcggcg tgaccggcga gcagcgccac
      121 ccattcgtcg ccgggctggt cgtaccaggc cccgggcggg gtactctggc cgcgcgacag
      181 gatgcgttcg atgcgcacgc cggggctccg gtacagcagc ccgaaggctt ccgcatcgag
      241 acgggcagga atgcccgcaa acagattgcc gctgtccggc tgttccatgt tcctcgcccc
      301 ctgatccatt cccctgccct gcatgtggca aggatgcacc gtttccgggc gccggataca
      361 ctcgcggccg tcttcctgcc cacgtcagcc gccacgctcc gggagcccgc cccatgctga
      421 tcgccttcct gatccgcgac ggtcgtctgg agcagacgcc gctgcacgtg caggacgacg
      481 cgccgatccc gccggacctc atgcagcaga tcgtctgggt cgatctcaac gagccgaccg
      541 aggaggagcg cgagtgggtc gaggcgcagt tcgggctgga actgcccgag taccgcgaga
      601 tgaatgatct cgaaccctcg gcgcgcctgt acgaggacga ggacggcctg cacctgcatt
      661 cgtacttcct gagcgatcag gaggaacagc cgcgcaacat cacggtcgcc ttcgccgtgc
      721 tcggcggccg gctgttcacg ctgcacgagg acgatctttc gaccttccgg ctgttccggc
      781 tgcgcgcgcg ccgccagatc gggctggtca aggatgtcgg cgacatcatc ctcggcctgt
      841 tcgaggccaa ggtcgaacac atcgccgaca agctcgaaga gatctactcc gaactcgaag
      901 ccctgagcca ccgcgtgctc gccgagaccg attcggacat ggaggacgtg ctgtcggggc
      961 tggccgtgca ggaggacatg aacggcaagg cacgcctgat cctgctcgac acgcgccgca
     1021 cgctgtcctt cctgctgcgc tacgcgatcg ccggccgcgg cgaggacatc cgcgagatcc
     1081 tgcgcgatgt cgaatcgctg accagccaca ccgccttcct gttcgacaag gtcaacttcc
     1141 tgatggacgc ggcgatgggc ttcatcaaca tcgagcagaa caagatcatc aagatcttct
     1201 cgatcgccgc cgtcgtgttc ctgcccccga cgctggtcgc ctcgctgtac ggcatgaact
     1261 tccagcacat gccggagctg gaatgggaat acggctaccc gatggcgatc tgcctgatga
     1321 tcctggcggg ggtggcgccg tactggttct tcaagaggaa gggctggctg tagcaggccg
     1381 ccttgcgctg ctgccgctca gccatccagc gcctgcacgc cctcgacacg agcactgcaa
     1441 cgtgccagcc acagatcgcg ctgtccctgc atccgcagcc agaaccccgc ggcttgcccg
     1501 gtcgcacatt ccagccgcag cgccatttcg gcgttgatcg cggcccggcc gtcgagcagc
     1561 gccgacaagc gctgccggct cactccgagc cgcctcgccg cctcgctgac cgacatgccc
     1621 gcaggcagat agtcacgcag cagttcgccg ggatgtgccg gatcgtgcat ttcacgtcac
     1681 ccgcctgaat cgccatccac cgcgatgatt tgtgcatcga tctcctccga tgccggttcg
     1741 atgcgcggat acacctgcct gccgtcccgg atcacatgat cgcggatgcg tccggccggc
     1801 aaccggtccc agtgcaggac atcgagcgtg aacaccaggg gcagatcgtc gagatcgttc
     1861 caaagctgcg cgaaggtctc gtcgtccatc tccggggcga ggacggcaag gtcgatgtcc
     1921 gaggcgttgc ggtaggtgcc tttcgcacgc gagccgaaaa ccagtacgcg ctcgatgccg
     1981 ggataacgcg cgaacacggt acgcagccat tcgagaatct cggctgacag gccgaagctc
     2041 agtccgtcat ctgccatgca cgggcctgta cggcgagccg tcgatacagt tccagcccct
     2101 cgccgacggc gaaggcgtac accgtttcgg cgagcgattc atcgtaggta tggctggtca
     2161 ggttgcgcat gcgctgcagg acgctccagt cgctgccatc gtcgatgaag ccggccttca
     2221 gcgcttcctg cagggtttcc ttcggtgtgc gcgccagaac cagttcggct tccagccgca
     2281 gtttcagcat cctccaggcg agttcatgcg tgaactcgaa acgctggatg accgaatcgc
     2341 gcaggaattc gtccttcggc tgccgggccg cgcgctccag ctgtccgagc gccttcagaa
     2401 aggcggcgat gcgttcctgc aggcgttgct gatccatccg gcttgcgtgc tcccgattct
     2461 gcgccgaccc agcaccggca gcgtccctgc acgccgccgg tgctgcgtcc ggtcagcctc
     2521 gctcagcgct ccagctgcga caggtcccgc accgcgccgc ggctcgccga ggtcgtcatc
     2581 gccgcgtagg ccttcagggc cgtcgacacg acgcgctcgc ggctgaccgg cttccaggcc
     2641 cgggcaccgc ttgcggtcat gcggctgcgg cgttcggaga gatccttgtc ggacacgacg
     2701 atgcggatgc tgcgcttcgg gatgtcgatc tcgatcggat cgccctcctc gaccagcgcg
     2761 atgttgccgc cctcggccgc ctcggggctg acgtggccga tcgacaggcc cgaggtgccg
     2821 cccgaaaagc gcccgtcggt gatcagcgcg caggccttgc cgaggcccct ggacttcagg
     2881 tagctggtcg gatacagcat ctcctgcatg cccgggccgc ccttcggacc ttcgtagcgg
     2941 atcagcacga catcgccggc gcggatctcg tcgttcagga tcgcggcgac ggcgtcctcc
     3001 tggctttcca tgatccgcgc cgggccggtg aacttcagga tcgactcatc gacgccggcc
     3061 gtcttgacga tgcagccgtc cggcgcgagg ttgccgaaca gcaccgccag accgccgtcc
     3121 tgcgaatagg cgtgcgcccg gtcgcggatg cagccgtttt tgcggtcggc atcggccgtg
     3181 ggccagcggc agtcctggct gaaggcttcc tgcgtcggaa tgccggccgg gccggcgaga
     3241 taacgcttgc gcgcggcgtc ggtgcaggtc gccacggcga tgtcgttggc ggcgagtgcg
     3301 gcggcgagcg tgtcgctgtg cacggtcttc acgtcgtggt gcagcaggcc ggcgcgatcg
     3361 agctcgccga ggatggccat gatgccgccg gcccggtgca cgtcctcgat gtggtacttc
     3421 tgcgtcgccg gcgcgacctt gcagaggttc ggcacgcgcc ggctcaggcg gtcgatgtcg
     3481 ctcatcgtga actgcacgcc gccttccttg gccgcggcca gcaggtgcag cacggtgttg
     3541 gtcgagccgc ccatggcgat gtcgagcgtc atggcgttct cgaacgcctt gaagctggcg
     3601 atttcacgcg gcagcacgcg cgcgtcgtcc tgctcgtaat agcggcgcgc cagatcgacg
     3661 atcaggtggc cggcttccac gaacagccgc ttgcggtcgg cgtgcgtggc cacgatcg
//
LOCUS       DAOQJH010000218         3705 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_15559_length_3705_cov_3.485753, whole genome shotgun sequence.
ACCESSION   DAOQJH010000218 DAOQJH010000000
VERSION     DAOQJH010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3705
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15559_length_3705_cov_3.485753"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..418
                     /locus_tag="PLQ18_06150"
     CDS             <1..418
                     /locus_tag="PLQ18_06150"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HNE01270.1"
                     /translation="NCHLKSMRAEHRRAEDYAKKQRHAQAAVIHAFCASVLALDPQAR
                     LIVAGDFNDVPGSKTLDLLKGERLANLIEDLPRHLRHTHRHGHHAQTLDHVLVSPALR
                     AARAEVLHVHTTAPAPQPSDHDPVRVELRDVQPLPR"
     gene            529..1488
                     /locus_tag="PLQ18_06155"
     CDS             529..1488
                     /locus_tag="PLQ18_06155"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HNE01271.1"
                     /translation="MPRVAIVMPYFQREAGILLRTLASVQAQTVDDWMLLVVDDASPV
                     PARSEIADLVETDPQRYRLIEQANTGPAGARNRGLDAVPADCEYVAFLDSDDAWSPDH
                     LENALTALAAGHDFYFADHLQLGATTPAFARGGRLRPEQHRPLSAPHLYAFTADLFEQ
                     TLTGNVIGTSTVVFRHAKYPAQRFHQNLVFAGEDYLFWMELARAGARPAFSTRCEATY
                     GRGVNVFAGSGWGSEHSLHRLCDEITFWRAIPRLFALTATQRAHVRQRTRILRRSLAL
                     DLLHRLLHRKSLHPGRLLRLSRQDPAALLLLLPNLVIGKLRRS"
     gene            complement(1508..2311)
                     /locus_tag="PLQ18_06160"
     CDS             complement(1508..2311)
                     /locus_tag="PLQ18_06160"
                     /inference="COORDINATES: protein motif:HMM:TIGR03694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM/exosortase system-associated
                     acyltransferase"
                     /protein_id="HNE01272.1"
                     /translation="MTPPPEAVSPLLQFGSYFDLALADPDDGGAPQQAADLIDRAQHL
                     RYEVYCREFGFEREEDCPGQRERDEYDARSLHCLLMHHASHHDAGTVRLVLSDPGRPQ
                     APMPFVAHCGASLFTDHPAHPSRLAPELCCEVSRLAVASTFRKRFGENESPIGQIDGI
                     DISAPELRTYPLISLALFVAATRLVAACGCLHAYAIMEPRLARRLAAAGLKFERIGND
                     VEYHGRRAAFYIHLDTALAATAALPGMSAMYEYALARLDPQIPPARRRT"
     gene            2433..3698
                     /locus_tag="PLQ18_06165"
     CDS             2433..3698
                     /locus_tag="PLQ18_06165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE01273.1"
                     /translation="MHADTRHPAPELAACSGVLTLELNTGPAGSVPPGPVSRASATQI
                     AEGIAADLGRLVPDLARFALVVPGALYDQTELLRPGFPLLAALDDVFRGTLLDGGFVP
                     GLVALGSDDGRAFTLPALNPARQPGAGPLLMLPFSLLGRPGDITELSMQLENSLLQSG
                     GVSVTTRQAVEAAFGLRAVNLSYATVGDLCALLKVQLEHAGFDGLWELLERALFRPQE
                     PADVSLAPGNRFLMSEGTVWTPFWTFDDWAQIGPGQERPADALAAGYVEWVRTQRQYL
                     MALSAWGLPVRMAIGEAFTECAGTTMRDLRAGAALDGDYLVETVLHVDNAGPERRLLV
                     TNQFDDEVGTLAYTVAALDADGCLLALEHHYPLSPRGLEAIAARLTERSRDFERIVRH
                     PGGLLIDAAGRCLESAPDPATEPAAGTRH"
BASE COUNT          555 a         1356 c         1231 g          563 t
ORIGIN      
        1 caactgccac ctgaagtcga tgcgcgccga gcaccgccgc gccgaggact acgcgaagaa
       61 gcagcgccat gcgcaggccg cggtgatcca cgccttctgc gcgtcggtgc tcgcactcga
      121 tccacaggcg cggctgatcg tcgccggtga cttcaacgac gtgccgggct cgaagacgct
      181 ggatctgctg aagggcgagc ggcttgcgaa cctgatcgag gatctgccgc ggcacctgcg
      241 ccacacgcac cgccacggcc atcacgcgca gacgctcgat cacgtgctgg tgtcgccggc
      301 gctgcgcgcg gcgcgcgccg aggtgctgca cgtgcacacg acggcaccgg ctccacagcc
      361 gagcgatcac gatccggtgc gggtcgagct gcgggacgtg cagccactgc cgcgttaatt
      421 ttcgtcggtg cacgcccggg cgcgcttaac ggcagcggat caccggccca gaatggcgca
      481 cggccgatcc ggccccctcc atgcatcgct cgccagcaca ggatcaccat gcccagagtc
      541 gccatcgtca tgccctactt ccagcgcgaa gccggcatcc ttctccgaac cctcgcgtcg
      601 gtgcaggcac agacggtgga tgactggatg ctgctggttg tcgatgacgc ctcgcccgtg
      661 ccggcgcgca gcgaaatcgc cgacctggtc gaaaccgatc cgcagcgtta ccgactgatc
      721 gagcaggcca acaccggtcc ggccggcgct cgcaaccgcg gactcgatgc cgtgccggcc
      781 gactgcgaat acgtggcctt tctggattcc gacgatgcct ggagcccgga ccatctcgag
      841 aacgccctga ctgccctcgc ggctggccac gacttctact ttgccgatca cctgcagctc
      901 ggtgcgacga cgccggcctt tgcgcgcggt ggccggctgc gacctgaaca gcaccggccg
      961 ctgtccgccc cgcatctgta tgccttcacg gcagatctgt ttgaacagac gctgaccggc
     1021 aacgtcatcg gcacgtcgac ggtggtcttc cgccatgcaa agtatcccgc gcagcgcttc
     1081 catcagaatc tggtctttgc cggcgaggac tacctgttct ggatggaact ggcccgggcc
     1141 ggagcccggc cggcgttttc gacccgctgc gaggccacct acggccgcgg cgtcaacgtg
     1201 ttcgccggct caggctgggg cagcgagcat tccctgcacc ggctgtgcga cgaaatcacc
     1261 ttctggcgtg ccataccgcg cctgttcgcg ctgacggcca cgcagcgtgc ccacgtccgt
     1321 cagcgcacgc gaatcctgcg acgctcactc gcactcgatc tcctgcaccg gctgctgcac
     1381 cgcaagtccc tgcatcccgg acgtctgctg cgcctttcgc gtcaggatcc ggcggcgctg
     1441 ctgctgctgc tgccaaacct ggtgatcggc aagctgcgcc gatcctgaaa cgcatgccgg
     1501 gcggcggtca tgtccgccgc cgcgccggcg gaatctgcgg atcgagccgc gccagcgcgt
     1561 attcgtacat cgcgctcatg cccggcagcg ctgcggtcgc cgccagcgcg gtgtcgaggt
     1621 ggatgtagaa ggctgcgcgc cggccgtggt actcgacatc attgccgatg cgctcgaact
     1681 tcagcccggc cgccgccagc cggcgcgcca ggcgcggctc catgatcgcg taggcgtgca
     1741 ggcagccgca ggccgccacc agcctcgtgg ccgcgacgaa cagcgccagc gagatcagcg
     1801 ggtaggtgcg cagctccggc gccgagatgt cgatgccgtc gatctggccg atcgggcttt
     1861 cgttttcacc gaagcgcttg cggaaggtcg aggcgaccgc cagccgcgac acctcgcagc
     1921 agagctccgg ggccagccgc gacggatgcg ccgggtgatc ggtgaacagg ctggcgccgc
     1981 agtgcgcgac gaagggcatc ggcgcctgcg gccggcccgg gtcggacagc accagacgca
     2041 cggtgccggc gtcgtggtgg ctggcgtggt gcatcagcag gcagtgcagc gagcgggcgt
     2101 cgtattcgtc gcgctcgcgc tggcccgggc agtcctcctc gcgctcgaaa ccgaattcgc
     2161 ggcagtagac ctcgtagcgc aggtgctggg cacgatcgat caggtccgcg gcctgttgcg
     2221 gcgcgccgcc atcgtcggga tcggcgagcg caagatcgaa gtaactgccg aactgcagca
     2281 gcggggaaac cgcttccgga ggaggcgtca tcagcaaact ccatcaaaaa ccacaggaat
     2341 caggtgaaat cctgtcacgg accggccgcg acggcttccg gtatagtagc cgccccgcaa
     2401 agaccggacc gcccgacgct ggagctttcg ccttgcacgc cgatacccgc caccccgccc
     2461 ccgaactcgc cgcctgctcg ggcgtgctga cgctggagct gaacaccggc ccggccggca
     2521 gcgtgccacc cggcccggtc agccgcgcca gtgccacgca gatcgccgaa ggcatcgccg
     2581 ccgacctcgg ccggctggtg cccgatctgg cgcgcttcgc gctggtcgtg cccggcgcgc
     2641 tgtacgacca gaccgaattg ctgcgcccgg gttttccgct gctcgccgcg ctcgatgacg
     2701 tgttccgcgg cacgctgctc gatggcggct tcgtgcccgg tctggtcgca ctcggcagcg
     2761 acgacggccg cgccttcacg ctgccggcgc tcaatcccgc ccgccagccc ggcgccggac
     2821 cgctgctgat gctgccgttc tcgctgctcg gccgccccgg ggacatcacg gaactgtcga
     2881 tgcagctcga aaactcgctg ctgcaatcgg gcggcgtgtc ggtgacgacg cgccaggcgg
     2941 tcgaagcggc cttcggcctg cgcgccgtga atctttccta tgcgacggtc ggcgacctgt
     3001 gtgcgctgct gaaggtgcag ctcgaacacg ccggcttcga cggcctgtgg gagctgctcg
     3061 aacgcgcgct gttccggccg caggagccgg ccgatgtgag cctcgcgccc ggcaaccgct
     3121 tcctgatgag cgagggcacg gtctggacgc cgttctggac cttcgacgac tgggcgcaga
     3181 tcggccccgg tcaggaacgc ccggccgatg cgctcgccgc cggctacgtc gaatgggtgc
     3241 gcacgcagcg ccagtacctg atggcgctgt ccgcctgggg cctgccggtg cgcatggcga
     3301 tcggcgaagc cttcaccgaa tgcgccggca cgacgatgcg cgacctgcgc gccggcgccg
     3361 cgctcgacgg ggattatctg gtcgagaccg tgctgcacgt cgacaacgcc ggcccggagc
     3421 gccggctgct ggtcaccaac cagttcgacg acgaggtcgg cacgctcgcc tacacggtcg
     3481 ccgcgctcga tgccgacggc tgcctgctcg cgctcgaaca ccactacccg ctgtcgccgc
     3541 gcgggctgga ggcgatcgcc gcgcgcctga ccgagcgcag ccgcgacttc gagcgcatcg
     3601 tccgccaccc cggcggcctg ctgatcgacg ccgccggccg ctgcctggaa tcggccccgg
     3661 acccggccac cgaacccgcc gccggcacgc gccactgagc ctgcc
//
LOCUS       DAOQJH010000219         3699 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_15601_length_3699_cov_4.604830, whole genome shotgun sequence.
ACCESSION   DAOQJH010000219 DAOQJH010000000
VERSION     DAOQJH010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3699)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3699)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3699
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15601_length_3699_cov_4.604830"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(172..540)
                     /locus_tag="PLQ18_06170"
     CDS             complement(172..540)
                     /locus_tag="PLQ18_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HNE01274.1"
                     /translation="MTEPSTATPAPASDPLPAGDPGPDSSPCTQCGACCATYRVSFYW
                     GEAPQLGLPGSLYEALSAWHACMRGTASAPMRCVALAGGIGQSVTCTVYAQRPSPCRE
                     LQAGDDRCQRARARHGLAAL"
     gene            779..1984
                     /locus_tag="PLQ18_06175"
     CDS             779..1984
                     /locus_tag="PLQ18_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002647207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding peptidase"
                     /protein_id="HNE01275.1"
                     /translation="MKPLYDVVSQGLIHSWKPVKAAKAGRAYRCRCSRPVFFRNSECL
                     ACHAPLGFEPLLGELGALQPGDAEGLWRLENGDGAQYRRCANFEQIGCNWLIPADSDA
                     TLCLSCVLNRTIPDLSEPENLAYWQSIEIAKRQLVAQLLALGLPVRSKVSDDPDNGLC
                     FDFLKSLPDGPRVLTGHDEGLITLNVEEADDARREAMRKQLGEPYRTLLGHLRHEVGH
                     YYWDVLVRDSHWLEPYRALFGDESFDYATALQQHYENGPPADWPQQFVSAYATSHPWE
                     DWAETWAHYLHLVDTMNTALAFGLNAEDVEVDTEPFGSDALYDPQHPGAGQFLYFINA
                     WVDLVTILNELSRSMGQRDFYPFVMSRPVVAKLHFIHLVIEDARDQHLQAQDGGVEAA
                     TTMAEPVVS"
     gene            complement(2025..2702)
                     /locus_tag="PLQ18_06180"
     CDS             complement(2025..2702)
                     /locus_tag="PLQ18_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002642057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4276 family protein"
                     /protein_id="HNE01276.1"
                     /translation="MIQVIIIAEGQTEETFVRDVMAPVFAASGIILTARLVEVAGGGR
                     GGALSYERVWKFLRNTLRERASTYVSTFFDLHKLDTAFPAHAGSLKISDSGRRAGYLE
                     TAFHADVIARTGCRPDRFIPHIQPWEFETLLFSDLTTLTGTETSWARHLPALQNIRSG
                     FAHPEQINGSQTTKPSARLGLLRNPGFRKVRHGPLAAGHIGLDRIKAECPHFAAWLQR
                     LRTLTPL"
     gene            complement(2699..>3699)
                     /locus_tag="PLQ18_06185"
     CDS             complement(2699..>3699)
                     /locus_tag="PLQ18_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017841331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNE01277.1"
                     /translation="LRDLNVLIGANGAGKSNFISLFRLLTALSEKRLQLYIQKQGGPD
                     ALLFRSRKHSEHLIATFNIEHDSGYCFSLAPTSDNRMVFDLEALWLNSPKTQNQLDEN
                     INPNTNGYFEAKAFQPFKWRVYHFHDTGDSARVKQLHAINDNLRLKPDATNLAAYLFR
                     LHNDHIDSYKRIVETIRLAAPFFGDFVHREPVNDSIQLEWTEYNDPDTPLKAHMLSDG
                     TLRFICLTTLLLQPTELLPDTILIDEPELGLHPYAIALLADMLREVSENTQLIISTQS
                     VELLNHFNAEDVIVVDRINDESVFRRLDTESLHDWLQDYSLGELWKQNVLGGRPSR"
BASE COUNT          663 a         1101 c         1222 g          713 t
ORIGIN      
        1 ggtgagcagg cgctgcagcg cctcgtcggc gcgggtttcg gcgtgctgct gcgcgtcggc
       61 ctggctggca tcggaccagc cgtatcggcg cacggtgatc tgccgcccat ccttgcgctg
      121 ttgccgtcgc gcttctgccc agtaggcggg gacgatcatc ggcgcgccgg ctcagagcgc
      181 tgccagtccg tgccgcgcgc gggcacgctg acagcggtca tcgccggcct gcagttcgcg
      241 gcacggcgac gggcgctgcg catagacggt gcaggtcacg gactgcccga tcccgccggc
      301 cagcgccacg cagcgcatcg gggctgacgc ggtgccgcgc atgcaggcgt gccaggcgct
      361 cagggcttcg tacagcgatc cgggcaggcc gagctgcggc gcttcgcccc agtagaacga
      421 cacgcgatag gtcgcgcagc aggcgccgca ctgcgtgcag ggcgaggaat cggggccggg
      481 gtcgccggca ggcagcggat cggatgccgg ggccggcgtg gccgtgctcg gttcggtcat
      541 ggcgttacgg tctgttcatg taggggaatt ctggcaccga acctcgcggt ccgcggtcgg
      601 agcgtcacgg tccggtctgg agggcagcgg cgcgcaggcg tcgtgcccgc agcattgccg
      661 gaacgcatgc cgatgttctc acgcatccgc acgcatcccc gaatccgggg agcacggtga
      721 aattttcgcc gtgctgcact ccgggcgggc gcgtgcggca ccacgccgga ggcgttcgat
      781 gaagccgctt tacgatgtcg tcagtcaggg tctgatccac agctggaagc cggtcaaggc
      841 tgccaaggcc ggccgcgcct accgctgccg ctgctcgcgc ccggtgttct tccgcaacag
      901 cgaatgcctc gcctgccacg caccgctcgg tttcgagccg ctgctcggcg agctgggcgc
      961 gctgcagccc ggcgatgccg agggcctgtg gcggctcgaa aacggcgacg gcgcgcagta
     1021 ccggcgctgc gcgaacttcg agcagatcgg ctgcaactgg ctgatcccgg ccgacagcga
     1081 cgcgacgctg tgcctgtcct gcgtgctgaa ccgcacgatc ccggacctca gcgagccgga
     1141 gaatctcgcg tactggcaga gcatcgagat cgccaagcgc cagctggtcg cgcaactgct
     1201 cgcactcggc ctgccggtgc gctcgaaggt cagtgacgac ccggacaacg gcctgtgctt
     1261 cgatttcctg aagtccctgc ccgacgggcc gcgcgtgctg accggtcacg acgagggcct
     1321 gatcacgctg aacgtcgagg aagccgacga cgccaggcgc gaggcgatgc gcaagcagct
     1381 cggcgagccc taccgcacgc tgctcggcca tctgcgccac gaggtcgggc attactactg
     1441 ggatgtgctc gtgcgcgact cgcactggct ggagccgtac cgtgcgctgt tcggcgacga
     1501 gtccttcgac tacgccaccg cgctgcagca gcactacgag aacggcccgc cggccgactg
     1561 gccgcagcag ttcgtcagtg cctacgcgac cagtcatccg tgggaggact gggccgagac
     1621 ctgggcgcat tacctgcacc tggtcgacac gatgaacacc gcgctcgcct tcggcctgaa
     1681 cgccgaggat gtcgaggtcg acaccgagcc gttcggctcc gatgcgctgt acgacccgca
     1741 gcaccccggc gccggacagt tcctgtactt catcaacgcc tgggtcgatc tcgtgacgat
     1801 cctgaacgag ctgtcgcgca gcatgggcca gcgcgacttc tacccgttcg tcatgagccg
     1861 gccggtggtg gcgaagctgc acttcatcca tctcgtcatc gaggacgccc gcgaccagca
     1921 cctgcaggca caggacggcg gggtcgaggc cgcgacgacg atggcggagc cggtggtgtc
     1981 gtgagccatg tccggcagag ggcggttgaa cgcctcctgc cggttcaaag cggcgtcagc
     2041 gtgcgcaggc gctgcagcca ggctgcgaaa tgcgggcatt cggccttgat gcgatcgagg
     2101 ccgatgtgac cggcggcgag cggaccatga cggaccttgc gaaagcccgg gttgcgcaac
     2161 agaccgaggc gagcggacgg ctttgtggtc tggctgccgt tgatctgttc gggatgagcg
     2221 aagcctgaac gaatattctg cagtgcaggc agatgcctgg cccaactcgt ctccgtgccg
     2281 gtcagggtgg tcagatcgga aaacaggagc gtctcgaact cccagggctg gatatgcgga
     2341 atgaagcggt caggccggca tccggtgcgt gcgatgacat ctgcatgaaa cgccgtttcc
     2401 agatacccgg cccgtctgcc gctatcggaa attttcagcg atccagcatg cgcaggaaag
     2461 gcagtgtcga gtttgtgcag gtcgaagaaa gtgctgacgt acgtgctggc gcgttcacgc
     2521 aaggtattgc gcaggaattt ccagacccgc tcatagctga gtgctccgcc acgaccaccg
     2581 cctgcaactt ccaccagccg cgctgtcagg ataatgccgc ttgccgcgaa taccggagcc
     2641 atcacgtcgc gtacgaaggt ctcttcggtc tggccttcgg cgatgatgat gacctggatc
     2701 accggcttgg tctcccgccg agcacgttct gcttccagag ttcgccgagc gaataatcct
     2761 gcagccagtc atgcaacgat tccgtatcga gccgtcgaaa gacggattcg tcgttgatgc
     2821 gatcgacgac gatgacatcc tcggcattga agtgattcag cagttcaacc gattgcgtgg
     2881 aaatgatcag ttgggtgttt tcggatactt cccgcaacat gtcggccagc agggcaatcg
     2941 catacgggtg caggccgagt tccggttcgt cgatcaggat ggtatcgggc agcagctcgg
     3001 ttggttgcag caacagcgtc gtcaggcaga tgaagcgtaa cgtgccgtcg gacagcatgt
     3061 gcgctttcag tggggtgtcg gggtcgttgt actcggtcca ttcgagttga atcgaatcgt
     3121 ttactggctc tcgatgtacg aaatctccaa agaatggtgc ggcgagacgg atggtttcca
     3181 caatgcgttt gtagctgtcg atgtggtcat tgtgcagtct gaaaagataa gcggctagat
     3241 ttgtcgcgtc tggctttaag cgaagattgt cgttgattgc atgaagttgc tttactcttg
     3301 cagagtcgcc ggtatcatga aagtggtaaa cgcgccattt aaaaggctgg aatgcttttg
     3361 cttcaaagta gccgttggtg ttggggttga tattctcgtc tagttgattc tgtgtctttg
     3421 gcgaatttag ccataaagct tcgagatcga aaaccatgcg attgtcactg gtaggtgcga
     3481 ggctgaagca gtagcctgag tcgtgctcaa tattaaaagt ggcgattaag tgttcggaat
     3541 gctttctgct cctgaatagt aaagcgtcag gaccgccttg tttttgaatg tatagttgca
     3601 gccgcttttc agacagggcc gtcaaaagac ggaacaggct gataaagttg cttttgccgg
     3661 cgccgttggc gccgatcaag acattcaggt cacgcagtt
//
LOCUS       DAOQJH010000220         3691 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_15656_length_3691_cov_5.010451, whole genome shotgun sequence.
ACCESSION   DAOQJH010000220 DAOQJH010000000
VERSION     DAOQJH010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3691)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3691)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3691
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15656_length_3691_cov_5.010451"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /locus_tag="PLQ18_06190"
     CDS             <1..187
                     /locus_tag="PLQ18_06190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE01278.1"
                     /translation="GRPHAQPARSRPAGGLNQPERPRHDTKSRFHEVRPLPRKTSGAS
                     TGEGKNERRHGCRDENE"
     gene            375..3233
                     /locus_tag="PLQ18_06195"
     CDS             375..3233
                     /locus_tag="PLQ18_06195"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005224734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HNE01279.1"
                     /translation="MSTSFDQFRQTSVLSGGNAAYIEDLYESFLQDPDSVSENWRAYF
                     LGLRAGGNGAAEHLHGPVREVFAKIGQNPRAIISLLPQLSAGESLNPEAAHKQAGVLR
                     LINAHRTRGHQAATLDPLALRERPAVPDLDLAEHGLSEADLDAGFDVGSLQVGKQQLP
                     LREILELVRRVYTGNIGSEYMHIHTTEEKRWIQHRLEGTGARLDLNAAEKKELLDWLV
                     AAEGLEKYLHTKYVGQKRFSLEGGDSLIPMMDALIQHAGASGAKEIVIGMAHRGRLNM
                     LVNILGKAPAELFKEFEGKKKTSAEISSGDVKYHMGFSSDVATPGGVVHLALAFNPSH
                     LEIVNPVVEGSVRARLERRKSGGQDSPLPFLEVLPVLIHGDAAFAGQGVNQELFQMAS
                     VRGYRTGGTVHIVVNNQIGFTTSNPLDVRSSTYCTDVAKIIQAPVFHVNGDDPEAVLF
                     VTKLAMDYRTTFGKDVVIDLVCYRRHGHNEADEPSATQPMMYKKIKARSTAATLYAEQ
                     LVREGVLADGEFTQRVADYRAKLDAGIQVSRPTLEGVSNPYAVDWAPYKNEDWSQPGE
                     TGFPMERLQQLAEKLQTIPDGHEPHARVAKIVDDRRKMAAGALALDWGFAENMAYATL
                     LEEGFQVRVSGEDCGRGTFFHRHAVLHSVVDGSAYIPLANVRPDQPTFTVIDSILSEE
                     AVLGFEYGYATAEPNGLIIWEAQFGDFANGAQVVIDQFISSGQTKWGRLCGLVMLLPH
                     GFEGQGPEHSSARLERYLQLCAEHNMQVIVPSTPAQCFHMLRRQCKRSFRKPAIVMSP
                     KSLLRHRLAVNTLEDLANGRFQEIIPEIDALDPAKVTRIVFCSGKVYYDLLEKRREQN
                     LENVAVVRIEQLYPFPKADYDTEIARYPNATDIVWCQEEPQNQGAWYQSRHHLEAGLS
                     GKHTLSYAGRAASASPAVGSHQVHVEQQTALVSQALGL"
     gene            3272..>3691
                     /locus_tag="PLQ18_06200"
     CDS             3272..>3691
                     /locus_tag="PLQ18_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007805254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="E3 binding domain-containing protein"
                     /protein_id="HNE01280.1"
                     /translation="MSVEVRVPNLPESVADATLVNWHKKPGDAVKRDENLVDLETDKV
                     VLEVPAPTDGVLGEILQADGSTVKAGDLIATIVAGAAANAPAAAPAPAAAAAPAAAGA
                     EVIDLDGLSPAVRKLITENKLDVAKIAGSGRNGRITKE"
BASE COUNT          698 a         1300 c         1174 g          519 t
ORIGIN      
        1 tggccgacca catgcgcaac cggcccgatc acgacccgcc ggtggcctga accagccgga
       61 gcgtccgagg cacgatacga aatccagatt tcacgaagtg cggccgctgc cgcgcaaaac
      121 ctccggtgcc agcaccggcg aagggaagaa cgaacgacgg cacggctgcc gcgatgaaaa
      181 cgaataaata aaccgaccgc gggacatacc gcggcgaaaa gagccgcagc aagacggcag
      241 gccggtcccg gatccggcga gtggtaccga aaaaatcagc atccaggtcg aggcctacga
      301 gcaccctgac cgagccgaag ctgtccggcg gttcgcacgg ggtttttcaa cgacggggag
      361 tcaacagaag ccaaatgagc acctcattcg atcagttccg gcagacttcg gtcctgtccg
      421 gcggcaatgc tgcgtatatc gaagacctct acgagagctt cctgcaggat cccgattcgg
      481 tttccgagaa ctggcgggcg tatttcctcg gcctgcgcgc cggcggcaac ggcgcggccg
      541 agcacctgca cgggccggtg cgcgaagtgt tcgcgaagat cggccagaac ccgcgcgcga
      601 tcatctcgct gctgccgcag ctcagcgccg gcgagtcgtt gaatcccgag gccgcgcaca
      661 agcaggccgg cgtgctgcgc ctgatcaacg cccaccgcac gcgcggccac caggccgcaa
      721 ccctcgaccc gctggcgctg cgcgagcgcc cggcggtgcc ggacctcgat ctcgccgagc
      781 acggcctgag cgaggccgat ctcgatgccg gcttcgacgt cggctcgctg caggtcggca
      841 agcagcagct gccgctgcgc gagattctcg agctcgtgcg tcgcgtgtac accggcaaca
      901 tcggctcgga gtacatgcac atccacacca ccgaggagaa gcgctggatc cagcatcgcc
      961 tcgaaggcac cggcgcgagg ctcgacctga acgcggccga gaagaaggaa ctgctcgact
     1021 ggctggtcgc tgccgagggc ctcgaaaagt acctgcacac caagtacgtc ggccagaagc
     1081 gcttctcgct cgaaggcggc gactcgctga tcccgatgat ggacgcgctg atccagcacg
     1141 ccggcgcgag cggtgcgaag gaaatcgtca tcggcatggc ccaccgcggc cgcctgaaca
     1201 tgctcgtgaa catcctcggc aaggcgccgg cggagctgtt caaggagttc gagggcaaga
     1261 agaagaccag cgccgaaatc tcctcgggcg acgtcaagta ccacatgggc ttctcgtcgg
     1321 acgtggcgac gccgggcggc gtcgtgcatc tggcgctcgc cttcaacccc tcgcacctgg
     1381 aaatcgtcaa cccggtggtc gagggctcgg tgcgtgcgcg tctggagcgg cgcaagtccg
     1441 gcggccagga ctcgccgctg ccgttcctcg aagtgctgcc ggtgctgatc cacggtgacg
     1501 cggccttcgc cggccagggc gtcaaccagg agctgttcca gatggcgagc gtgcgcggct
     1561 accgcaccgg cggcaccgtg cacatcgtcg tcaacaacca gatcggcttc acgacctcga
     1621 acccgctcga cgtgcgttcg tccacgtact gcaccgacgt cgccaagatc atccaggcgc
     1681 cggtgttcca cgtgaacggc gacgatcccg aggccgtgct gttcgtcacc aagctggcga
     1741 tggactaccg cacgaccttc ggcaaggacg tggtcatcga tctggtctgc taccgccgcc
     1801 acggccacaa cgaggccgac gagccctcgg ccacgcagcc gatgatgtac aagaagatca
     1861 aggcccgctc gaccgccgcg acgctgtatg cggagcagct cgtgcgcgag ggcgtgctcg
     1921 ccgacggcga gttcacgcag cgcgtcgccg actaccgcgc caagctcgac gccggcatcc
     1981 aggtctcgcg cccgacgctc gaaggcgtct cgaacccgta cgcggtcgac tgggcaccgt
     2041 acaagaacga ggactggtcg cagcccggcg agaccggctt cccgatggag cgcctgcagc
     2101 agctcgccga gaagctgcag accatccccg atggccacga gccgcacgcc cgcgtcgcca
     2161 agatcgtcga tgaccgccgc aagatggccg ccggtgcgct ggccctcgac tggggcttcg
     2221 ccgagaacat ggcctacgcg acgctgctcg aagaaggctt ccaggtgcgc gtctccggcg
     2281 aggactgcgg tcgcggcacg ttcttccacc gccatgcggt gctgcacagc gtcgtcgacg
     2341 gctcggccta catcccgctc gccaacgtca ggcccgacca gccgaccttc acggtcatcg
     2401 actcgatcct gtccgaagag gccgtgctcg gcttcgagta cggctacgcg accgccgagc
     2461 cgaacggcct gatcatctgg gaagcgcagt tcggtgactt cgccaacggc gcgcaggtgg
     2521 tcatcgacca gttcatctcc tcgggccaga ccaagtgggg ccggctctgc ggcctggtga
     2581 tgctgctgcc gcacggcttc gagggtcagg gtccggagca ttcctcggcg cgtctggagc
     2641 gctacctgca gctgtgcgcc gagcacaaca tgcaggtcat cgtgccgtcg acgccggcgc
     2701 agtgcttcca catgctgcgt cgccagtgca agcgcagctt ccgcaagccg gcgatcgtca
     2761 tgagcccgaa gagcctgctg cgtcaccgcc tggcggtgaa cacgctcgaa gacctcgcca
     2821 acggccgctt ccaggagatc atcccggaga tcgacgccct cgatccggcc aaggtcacgc
     2881 gcatcgtgtt ctgctcgggc aaggtctact acgacctgct cgaaaagcgt cgcgagcaga
     2941 acctcgagaa cgtcgcggtc gtgcgcatcg agcagctcta cccgttcccg aaggccgact
     3001 acgacaccga gatcgcgcgc tatccgaatg ccaccgacat cgtctggtgc caggaagagc
     3061 cgcagaacca gggtgcgtgg taccagagcc gccatcacct cgaagccggc ctgagcggca
     3121 agcacacgct cagctacgcc ggccgtgccg cctcggcctc gccggccgtc ggctcgcacc
     3181 aggtccacgt cgaacaacag accgctctcg tcagccaggc cctcggcctc tgagccccgg
     3241 cccataacca gatcaacagg gaataacgag aatgagcgta gaagtccgag ttccgaacct
     3301 ccccgagtcc gtcgccgacg ccacgctggt caactggcac aagaagccgg gtgatgcggt
     3361 caagcgcgac gagaacctcg tcgatctcga aaccgacaag gtcgtgctcg aagtgccggc
     3421 gccgaccgac ggcgtgctcg gcgagatcct gcaggccgac ggctcgaccg tgaaggccgg
     3481 cgacctgatc gcgaccatcg tcgccggagc cgccgccaac gccccggccg ccgcaccggc
     3541 accggccgcc gcggccgcac cggctgccgc cggcgccgag gtcatcgacc tcgacggcct
     3601 gtcgccggcc gtgcgcaagc tgatcaccga gaacaagctc gacgtcgcga agatcgccgg
     3661 ctccggccgc aacggccgca tcaccaagga a
//
LOCUS       DAOQJH010000221         3689 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_15678_length_3689_cov_2.621629, whole genome shotgun sequence.
ACCESSION   DAOQJH010000221 DAOQJH010000000
VERSION     DAOQJH010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3689)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3689)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3689
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15678_length_3689_cov_2.621629"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..918
                     /locus_tag="PLQ18_06205"
     CDS             <1..918
                     /locus_tag="PLQ18_06205"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNE01281.1"
                     /translation="YVLPRFIGAQPVLLLAALALVSVIVLAVSVLAVRGLTRPLARLA
                     GAARELGTDLERPPLPETGPVEVRDAAIAFNDMQARLRRQLAERDQLLAAVSHDLKTP
                     LARLRLRLELIGESGEQARALADLADLETTVADTLDFLRAGREEATVPVAIGALAEAL
                     ADDAADTGAAVEVLGDGGAPLPARPRALRRCLQNLLDNALRYGNSDGEADAPVEIVLH
                     DAPQALCICIRDRGPGLPAAELERVFEPFRRLEHSRSRATGGTGLGLSIARAIARGHG
                     GELTLANRSDGPGLEARLTLPRRPLAGAR"
     gene            1069..>3689
                     /locus_tag="PLQ18_06210"
     CDS             1069..>3689
                     /locus_tag="PLQ18_06210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNE01282.1"
                     /translation="MGPDADTPEGRAARVDRELAATLDCLDQALLTFDGTLCLRRYSA
                     RAARLFGLSDDALGRPASGLDGLATFPGLVRRLETVLTVGGRYADELHSDRAVWLLQI
                     TRIDADDPQAGLVCALIETTPLRAAHRALIESEERYALAVEAAQDGLWEWLPAEDALF
                     LSPRLCTLLGCSDAGRPASGAALRERIDPADRPRYDEALAAHLSGRTPAVQIELRMLR
                     DDGTRLWVELHALARRDGAGRAMRVAGSIRDIADRRRTEAALRNSEEALSRTFAHAPI
                     GMAVVDLEGRFQRVNRELALITGYEESELLGRNFRAITHPDDLERDLALVEALLTGER
                     ESYTLEKRYLRRDGRAVWVLLSVAIVRDTAGRPLHFTAQMVDISARKDIEQALAAAEQ
                     RWHYALEGSGLGVWDWNAETDRIFYSPRWKEMLGYADDEIGDTLDEWEQRLHPEDREA
                     VHAALKLHYAGGTDYYEAEYRLRCRDGGWKWILDRGQVLERDADGRPLRAFGTHLDID
                     KRKHAEAALAEALRAASAAAAKSVFLANMSHEIRTPLNAVLGLARLAERRAVDPLQRD
                     WLTRLRGAGATLLALVDDILDASRIEAGGLSLERTHFTLDTVLSAVADQHTGAARAQG
                     LEFTIDAAPGTPARWLGDPLRVQQILGNLVDNAVKFTAQGRVGVEVSVRTREGCAQLC
                     LAVEDTGIGIDPARIEKLFDAYAQGDSSMTRRYGGSGLGLTIARRLAEAMGGTIEVDS
                     SPGRGSRFTACLALEADEPAPAMPLLAGRTLCIQGPPGPVGAQLRRLAAEHGARLVSD
                     PVAADWLLLEAAAVAAQAPPAGSRTRCAALVWRAAASPETAEPAWPTHLVDAVVHLPC
                     TPWSLARELAEAPDRPQP"
BASE COUNT          476 a         1424 c         1325 g          464 t
ORIGIN      
        1 tacgtgctgc cgcgcttcat cggcgcgcag ccggtgctgc tgctggcggc gctggcgctg
       61 gtcagcgtca tcgtgctcgc ggtgtcggtg ctcgccgtgc gcgggctgac ccggccgctg
      121 gcgcggctcg ccggcgcggc gcgcgaactc ggcaccgatc tcgagcggcc gccgctgccg
      181 gaaaccgggc cggtcgaggt gcgtgacgcg gcgatcgcat tcaacgacat gcaggcgcgg
      241 ctgcgccgcc agctcgccga gcgcgaccag ctgctcgccg ccgtctcgca cgacctgaag
      301 acgccgctgg cgcgcctgcg cctgcgcctg gagctgatcg gcgaatccgg cgagcaggcg
      361 cgcgcgctcg ccgatctcgc cgatctcgaa accacggtcg ccgacacgct cgacttcctg
      421 cgcgccgggc gcgaggaggc gacggtgccg gtcgcgatcg gcgccctcgc cgaggcgctc
      481 gccgacgatg ccgccgacac cggcgcggcg gtcgaggtgc tcggcgacgg cggcgcaccg
      541 ctgccggcac gcccgcgggc gctgcgccgc tgcctgcaga acctgctcga caacgcgctg
      601 cgctacggca acagcgatgg cgaggccgac gccccggtcg agatcgtgct gcacgatgcg
      661 ccgcaggcgc tgtgcatctg catccgcgac cgcggccccg gcctgccggc ggccgaactc
      721 gaacgcgtgt tcgaaccgtt ccggcgcctc gaacactcgc gctcgcgcgc caccggcggc
      781 accggcctcg ggctgtcgat cgcccgcgcc atcgcccgcg gccatggcgg cgagctgacg
      841 ctcgccaacc gcagcgatgg ccccggcctc gaagcccgcc tgacgctgcc gcgccgcccg
      901 ctcgctggcg cccgctgagc cgcgcgtgcc cttcccttcg tcccctgccc tgccgggcct
      961 gactgccact tgcggcggct gcgccgatca cggccgcacg gtttagactc cgcgcgccgg
     1021 cgcgccctcg cgccggcacc gtatccggat gacatgcgga gagaatctgt gggtcctgat
     1081 gccgataccc ccgaaggccg cgccgcccgc gtcgaccgcg agctggccgc gaccctcgac
     1141 tgtctcgacc aggcgctgct gaccttcgac ggcacgctct gcctgcgccg ctacagcgcg
     1201 cgcgccgccc ggctgttcgg gctgtcggac gacgcgctcg ggcggcccgc aagcgggctg
     1261 gacgggctgg cgacgtttcc gggcctggtg cgccggctgg agaccgtgct gacggtcggc
     1321 ggccgctatg ccgatgaact gcacagcgac cgcgccgtgt ggctgctgca gatcacgcgc
     1381 atcgacgccg acgatccgca ggccgggctg gtctgcgcgc tgatcgagac gacgccgctg
     1441 cgcgccgcgc accgggcgct gatcgagtcc gaggagcgct acgcgctcgc cgtcgaggcc
     1501 gcgcaggacg ggctctggga atggctgccg gccgaggacg cgctgttcct gtccccgcgc
     1561 ctgtgcacgc tgctcggctg cagcgacgcg gggcgaccgg ccagcggcgc ggcgctgcgc
     1621 gaacgcatcg atccggccga ccggccgcgc tacgacgagg cgctggccgc gcacctgagc
     1681 gggcgcacgc cggccgtgca gatcgagctg cgcatgctcc gcgacgacgg cacgcggctg
     1741 tgggtcgagc tgcacgcgct cgcgcggcgc gacggcgccg ggcgcgccat gcgcgtcgcc
     1801 ggctcgatcc gcgacatcgc cgaccggcgg cgcaccgagg cggcgctgcg caacagcgag
     1861 gaggccctga gccgcacctt cgcgcacgcg ccgatcggca tggcggtggt cgatctcgaa
     1921 ggccgcttcc agcgcgtcaa ccgcgagctg gcgctgatca ccggctacga ggaatccgag
     1981 ctgctcgggc gcaacttccg cgcgatcacg catccggacg acctcgagcg cgacctcgcg
     2041 ctggtcgagg cgctgctgac cggcgagcgc gagagctaca cgctggaaaa gcgctatctg
     2101 cgccgcgatg gccgcgcggt ctgggtgctg ctgtcggtgg cgatcgtgcg cgacaccgcc
     2161 ggccggccgc tgcacttcac cgcgcagatg gtcgacatct cggcgcgcaa ggacatcgag
     2221 caggccctcg ccgcggccga gcagcgctgg cactacgcgc tcgaaggcag cggcctcggc
     2281 gtctgggact ggaacgccga aaccgaccgc atcttctatt cgccgcgctg gaaggaaatg
     2341 ctcggctacg ccgatgacga gatcggcgac acgctcgatg aatgggaaca gcgcctgcac
     2401 cccgaggacc gcgaggccgt gcacgccgcg ctgaagctcc attacgccgg cgggaccgac
     2461 tactacgagg ccgaataccg gctgcgctgc cgcgacggcg gctggaagtg gattctcgac
     2521 cgcggccagg tgctcgagcg cgacgccgac ggccggccgc tgcgcgcctt cggcacgcac
     2581 ctcgacatcg acaagcgcaa gcacgccgag gccgcgctcg ccgaggcgct gcgcgcggcc
     2641 agcgccgccg ccgccaagag cgtgttcctg gccaacatga gccacgagat ccgcacgccg
     2701 ctgaacgcgg tgctcgggct tgcgcgcctc gccgaacgcc gggccgtcga tccgctgcag
     2761 cgcgactggc tgacgcggct gcgcggggcc ggcgcaacgc tgctggcgct ggtcgatgac
     2821 atcctcgatg cctcgcgcat cgaggccggc ggcctgtcac tcgaacgcac gcatttcacg
     2881 ctggacacgg tgctgtcggc cgtcgccgac cagcacaccg gcgcggcgcg cgcgcagggg
     2941 ctggaattca cgatcgacgc ggctccgggc acgccggcgc gctggctcgg tgatccgctg
     3001 cgcgtgcagc agatcctcgg caacctcgtc gacaacgccg tgaaattcac cgcccagggg
     3061 cgggtcggcg tcgaggtgtc ggtgcgcacg cgcgagggct gcgcgcagct ctgcctcgcc
     3121 gtcgaggaca ccggcatcgg catcgatccg gcgcgcatcg agaagctgtt cgatgcctac
     3181 gcgcagggcg acagctcgat gacgcgccgc tacggcggct ccggcctcgg cctgaccatc
     3241 gcccggcggc tcgccgaggc catgggcggc acgatcgagg tcgacagcag ccccggccgc
     3301 ggcagccgct tcacggcctg tctcgcgctc gaagccgacg aacccgcccc ggcgatgccg
     3361 ctgctcgccg ggcgcaccct ctgcatccag gggccgcccg gcccggtcgg cgcgcagctg
     3421 cgccggctcg ccgccgagca cggcgcgcgg ctggtctccg atccggtcgc cgccgactgg
     3481 ctgctgctcg aagccgcggc cgtcgccgcg caggcaccgc ccgccggcag ccgcacgcgc
     3541 tgcgcggcgc tggtctggcg cgccgccgcc tcgcccgaaa ccgccgagcc ggcgtggccg
     3601 acgcatctgg tcgatgccgt cgtgcatctg ccctgcacgc cgtggagcct cgcccgcgaa
     3661 ctcgccgaag ccccggatcg cccgcaacc
//
LOCUS       DAOQJH010000222         3677 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_15766_length_3677_cov_5.083379, whole genome shotgun sequence.
ACCESSION   DAOQJH010000222 DAOQJH010000000
VERSION     DAOQJH010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3677)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3677)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3677
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15766_length_3677_cov_5.083379"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1158)
                     /locus_tag="PLQ18_06215"
     CDS             complement(<1..1158)
                     /locus_tag="PLQ18_06215"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNE01283.1"
                     /translation="MNRSRLQPLALALVFALGGPPAVAGTATEELHAGLQSLDRNDYA
                     GAEIHLKNALQQRPDLAPAQVALGHTYLRQGRFELAESTLQGALRLGAERADIDPLRM
                     YLKLRQGEFQAVLDGFDPYRHTGAARARMLRLRGEARLELGLMEEAALSFTAAREAAP
                     QAPDGDIGLSMLALQQQDAALARSAAQRATGLDPRSTEAWTALAAAELAAGHATEALA
                     AADTALKLDELCLPARAARVSALIALGRADEAQPVLDRLRRQNPADVRTLLLAAELAR
                     ARQDPETARARLTDASLLLKAIPRETLLRSPSLLKLSGTVHEALGLSEKARGDLTQYL
                     ERMPGDETARRLLAAVLLRLGDARQALELLQPLPDDPHAVSDIRLLTLLADA"
     gene            complement(1249..2334)
                     /locus_tag="PLQ18_06220"
     CDS             complement(1249..2334)
                     /locus_tag="PLQ18_06220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HNE01284.1"
                     /translation="MNQRMLAAAIGAACLIGGVPTTQAAFFLPTPQFNAFQYGDFYSY
                     SLPILAYRYAREFGGGVGPGNPFYIASGPGQISDLTVIGTGANGQPVNTNYSGVDNAY
                     QTPNNTGVDQFQTTTSAPGGVDDPGDNGNPANHTTEFVGDGENTWDARVSALTSFLNG
                     GDLLFFFNNNQVNSGAATNQNLMAWGQFTLKDDAGVAPDLVFDLTNNRGAGGNPSLTG
                     AETLGYTHGAGDTPALNAAPASITTTDFYYILSGGQVTIPSADTCRSIFGYQIPGCVP
                     DADQTFNHNLGANQAAYMLYSPELNTAIANGLYDVMQFDFRMSALNNGYEQIFIASSS
                     SVTSVPEPASLALLGLTLPWLMRRRRR"
     gene            2664..>3677
                     /locus_tag="PLQ18_06225"
     CDS             2664..>3677
                     /locus_tag="PLQ18_06225"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010325359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HNE01285.1"
                     /translation="MPYRLLRPLLFRLDAERAHDLTLAALGRIGPAAGLMTRAVPRRP
                     RVVMGIEFPNPVGLAAGLDKDGSAIRGLAALGFGCLEIGTVTPRPQAGNPRPRLFRLP
                     EAQALINRYGFNNGGVTALVNRVMAARAAGFRGVLGINIGKNKDTPNERASDDYLLCL
                     QQVYAHADYVAVNISSPNTPGLRDLQYGEALDRLLDELKEAQTRLADHHGRYVPLALK
                     IAPDVADADLPVIAAALVRARLDAVIATNTTFSRAGVEHLRHAGETGGLSGAPLRERS
                     TAVIAGLAKALDGALPVIGVGGILSGDDARAKIEAGASLVQLYSGFIYRGPDLVSEAA
                     GTIA"
BASE COUNT          560 a         1163 c         1363 g          591 t
ORIGIN      
        1 ggcatcggcg agcagggtca gcaggcggat gtcgctgacg gcatgcggat cgtccggcag
       61 cggctgcagc agttccagtg cctggcgggc atcgccgagg cgcagcagca ccgcggcgag
      121 cagccggcgt gcagtctcgt cacccggcat gcgctccaga tactgcgtca ggtcgccgcg
      181 ggccttttcg gacaggccga gcgcctcgtg cacggtgccg gacaatttca gcagcgaggg
      241 cgagcgcagc agcgtctcgc gcgggatggc cttcagcagc agcgaggcat cggtcaggcg
      301 tgcgcgtgcc gtttccgggt cctggcgcgc gcgggccagt tcggcggcga gcagcagcgt
      361 gcgcacatcg gccggattct gccggcgcag gcgatcgagc accggctgcg cctcgtcggc
      421 acgcccgagc gcgatcaggg cgctgacgcg cgcggcgcgc gccggcaggc agagttcatc
      481 cagtttcagg gcggtgtcgg cggcggcgag ggcctcggtg gcatgcccgg cggcgagttc
      541 ggcggcggcg agcgcggtcc aggcttcggt cgagcgcgga tcgaggccgg tggcgcgctg
      601 ggcggcgctg cgcgcgagcg cggcatcctg ctgctgcagg gccagcatcg acaggccgat
      661 gtcaccgtcc ggcgcctgcg gtgcggcctc gcgggcagcg gtgaacgaca gggcagcctc
      721 ttccatcagg ccgagttcga gccgcgcctc accgcgcagg cgcagcatgc gcgcgcgcgc
      781 ggcgccggta tggcggtacg gatcgaagcc gtcgaggacg gcctggaact ctccctggcg
      841 cagcttcagg tacatgcgca gcggatcgat gtcggcgcgc tcggctccga ggcgcagcgc
      901 cccctgcagg gtgctttccg cgagttcgaa ccgcccctgg cgcagatagg tgtgaccgag
      961 cgcgacctgg gccggagcga ggtccgggcg ctgctgcaag gcattcttca gatggatttc
     1021 ggccccggcg taatcgttgc ggtcgaggct ctgcaggccg gcgtgcagtt cttcggtggc
     1081 ggtgccggcg acggccggcg gaccgcccag ggcgaagacc agcgccagcg ccagcggctg
     1141 gaggcgggag cggttcatcg gcagtttcct ggcacggatg gcagcggaca ccccctggcc
     1201 cgcaaggcgg gcgagggggg cgggcagcga cggggagcgc gcaggcgctc agcgacgccg
     1261 gcggcgcatc agccagggca gcgtcagtcc gagcagcgcg aggctggccg gttccgggac
     1321 ggaggtcacg ctgctggagc tggcgatgaa gatctgctcg taaccgttgt tcagggccga
     1381 catgcggaag tcgaactgca tcacgtcgta gagaccattg gcgatcgcgg tgttgagttc
     1441 gggcgaatac agcatgtagg cggcctggtt ggcgccgagg ttgtggttga aggtctggtc
     1501 ggcatcgggc acgcagcccg ggatctggta gccgaagatg ctgcggcagg tgtcggcact
     1561 cgggatcgtg acctgaccgc cggagaggat gtagtagaag tcggtcgtgg tgatgctggc
     1621 cggagcggcg ttgagggccg gggtgtcacc ggcgccgtgc gtgtagccca gcgtctcggc
     1681 gccggtcagc gaaggattgc cgccggcacc gcggttgttg gtcaggtcga agaccagatc
     1741 cggcgcgacg ccggcatcgt ccttcagcgt gaactgaccc caggccatca ggttctggtt
     1801 ggtggccgcg ccggaattga cctggttgtt gttgaagaag aacagcaggt caccgccgtt
     1861 caggaagctg gtcagcgccg agacgcgcgc atcccaggtg ttttcgccgt caccgacgaa
     1921 ctcggtggtg tgattcgccg gattgccgtt gtcacccggg tcatcgacac cgcccggagc
     1981 cgaggtcgtg gtctggaact ggtcaacgcc ggtgttgttc ggcgtctggt aggcattgtc
     2041 gacgccgctg taattcgtat tgaccggctg gccgttcgcg ccggtgccga tgacggtcag
     2101 atcggaaatc tgtccggggc cggaggcgat gtagaacgga ttgccggggc cgacgccacc
     2161 tccgaattcg cgcgcataac gatatgcgag gatcggcagc gagtacgaat agaagtcgcc
     2221 gtactggaag gcgttgaact gcggggtcgg caggaagaag gcagcctggg tcgtcggaac
     2281 gccgccgatc agacaggccg ctccgattgc ggctgccagc atgcgctggt tcatgttgct
     2341 cacctcatgt gcatgggttt tgcgcggatt tcttatgtgc tgcgatacat ggctttcacc
     2401 tccgctgtgt gaatcggctg gccggccctg acctggcgca cgcgggttct cgtgctgcgc
     2461 cagtgatccc gaatgttaaa tacagcaagt tacgtgccgt aagcttgctt acatgaaaaa
     2521 acaaggggtt tggtctgacc ggcgggcaaa ttaggtcgca cagctgataa aaatcctgac
     2581 agaccccggc ccacgaccac ggtcgggggc ggcggcaccg tgcttatact gatcggcccc
     2641 gagctgccga tgtgccgacc gccatgccct atcgcctgct gcgcccgctg ctgttccggc
     2701 tcgatgccga acgtgcccac gacctgacac tggctgcgct cgggcgcatc gggccggcgg
     2761 cgggactgat gacacgcgcg gtgccgcgcc ggccgcgcgt ggtcatgggc atcgagtttc
     2821 cgaatccggt cggtctggct gccggcctcg acaaggacgg ctcggcgatc cgcgggctgg
     2881 cggccctcgg gttcggctgc ctggagatcg gcacggtcac gccgcgcccg caggccggca
     2941 atccgcgccc gcggctgttc cggctgcccg aggcgcaggc gctgatcaac cgctacggct
     3001 tcaacaacgg cggcgtcacg gcgctggtca accgggtcat ggcggcacgc gcggccggct
     3061 ttcgcggcgt gctcggcatc aacatcggca agaacaagga cacgccgaac gagcgcgcca
     3121 gcgacgacta cctgctctgc ctgcagcagg tctacgcgca cgccgattac gtggccgtca
     3181 acatttcctc gccgaacacg ccgggcctgc gcgacctgca gtacggcgag gcgctcgacc
     3241 ggctgctcga cgaactcaag gaggcgcaga cccgcctcgc cgaccatcac ggccgctatg
     3301 tgccgctggc gctgaagatc gcgccggatg tggcggatgc cgacctgccg gtgatcgctg
     3361 cggcgctggt gcgggcacgt ctggatgcgg tgatcgcgac caacacgacc ttctcgcgtg
     3421 ccggcgtcga gcacctgcgc cacgccggcg agaccggcgg gctgtcaggg gcgccgctgc
     3481 gcgagcgttc gacggcggtg atcgccggac tggcgaaggc gcttgatggt gcgctgccgg
     3541 tcatcggcgt cggcggcatc ctgagcggcg atgacgcccg ggccaagatc gaggccgggg
     3601 ccagtctggt gcagctgtac tcgggcttca tctaccgcgg accggacctc gtcagcgaag
     3661 cggcaggaac gatcgcc
//
LOCUS       DAOQJH010000223         3676 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_15771_length_3676_cov_3.183927, whole genome shotgun sequence.
ACCESSION   DAOQJH010000223 DAOQJH010000000
VERSION     DAOQJH010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3676
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15771_length_3676_cov_3.183927"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..658
                     /locus_tag="PLQ18_06230"
     CDS             <1..658
                     /locus_tag="PLQ18_06230"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNE01286.1"
                     /translation="LAARLARLDADAALAEARRDWQALTGLDALPRRIDERAAQEPQL
                     HPERLAALAASRAAAGRVELAGRSAEERTKLRLGVRGERAARGEDGDYSLAVAVSIPF
                     GGERFRRAEVESARAELAEAAAEEARLADTLPGALAAVRAQLALERERLAALQTRQSL
                     ARERSRLAGRAHDLGQLDLPALLQARAAAIEVEAEAELQGLRVARAVARLNQTQGVLP
                     "
     gene            655..2388
                     /locus_tag="PLQ18_06235"
     CDS             655..2388
                     /locus_tag="PLQ18_06235"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HNE01287.1"
                     /translation="MSRQDWRQHLPRTPSLLTSVLPALALLVLTLAAPAPVSAHGGED
                     HSHEAAAAAPALPVALAPRFEATSPAFQLLGILENGRLTLYLDRFADNAPVAGATLTV
                     EVDGRTQPAPETGAATYALAETGALANPGTHELLVSVTAGDEQDVLIGTLSVPPAAAS
                     GAAAPAAAGGIGGIGGPGWGLAGLALLLGYGLGRLGRRTAPASPLLIAGALGLGLALA
                     ALPPAAHAHGGEDHGHDEAAPLPATLTGDAPRRLPDGSVFVPKPVGRLLGLRTQLAAM
                     ATLAQTVVVPGHLIADPNRSGRVQAPQAGRIVPAAGGIAHLGQPVRAGEVLAWLEPLA
                     ASLEAAGQSAGLAELDSQIELATQRLTRLQQLAGSVAQREIDEARTELAGLRKRRSAL
                     GTGLGGREPLRAPVDGVVSRSTLVAGQIVEARETLVEILDPGAWWVEAVVFDAGLAGN
                     IDAALAETDNGRTYRLGFLGMGFERRAQGLPMQWRVDGDGRGLSAGQPVRLLLQTRSR
                     SRGVPLPVAALVRGADGESRVWVQAGIERFVPRRVQVQPLDGERVLVTGGLADGERFL
                     VIGASLLAQLR"
     gene            2405..>3676
                     /locus_tag="PLQ18_06240"
     CDS             2405..>3676
                     /locus_tag="PLQ18_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012453796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNE01288.1"
                     /translation="MFDRLIRTSLALRLPTLVAALVLLVWGVQLARELPVDVFPDLNR
                     PTVTLMTEAEGMAPEEVESLVTVPLETAMNGLAGVRRVRSVSGVGLSVLYVEFDWGTD
                     VFRNRQQVAERIGSVQAQLPPGIVPQMGPVTSIMGEIMLIALKAGDGSGDGHGGEAVT
                     PMQLRDLADWTLRPQLLAIAGVAQAIPIGGEVRQIQVVPDPALMEDLDVTPQQLVEAL
                     QGFGANVSGGFLDHGGREVLIRALGRSARIADLEGLVVAVRDDRPVYLKQLASVRVAA
                     RTKRGDASVDGRPGVIVSVQKQPSADTVDLSRRIDATLANLARTLPPGVQVEVLFRQA
                     NFISAAVGNVEDALRDGAIMVAIVLFLFLLNVRTTLISLTAIPLSLLVAVLVFEAFGL
                     SINTMTLGGLAIAIGGLVDDAVVGVENVLRRL"
BASE COUNT          440 a         1358 c         1406 g          472 t
ORIGIN      
        1 cctcgcggcg cggctggcgc gcctcgatgc cgatgccgcg ctcgccgagg ccaggcgcga
       61 ctggcaggcg ctgaccggcc tcgatgcgct gccgcggcgt atcgacgagc gtgcggcaca
      121 ggagccgcag ctgcatccgg agcgcctcgc cgcgctggcc gcgagccggg ccgccgccgg
      181 tcgcgtcgag ctggccggac gcagcgccga ggagcgcacg aagctcaggc tcggcgtgcg
      241 cggcgaacgc gccgcccgcg gcgaggacgg ggactacagc ctggccgtgg ccgtcagcat
      301 cccgttcggc ggcgagcgtt tccgccgcgc cgaggtcgag agcgcgcgcg ccgagcttgc
      361 cgaggccgcg gccgaggaag cgcggctcgc cgacacgctg cccggcgcgc tcgccgcggt
      421 acgcgcacag ctcgcgctcg aacgcgaacg gctggcggcg ctgcagacgc gccagtcgct
      481 cgcgcgcgaa cgcagccgtc tcgccgggcg cgcccacgac ctcggccagc tcgacctgcc
      541 ggcgctgctg caggcgcgcg ccgccgccat cgaggtcgag gccgaagccg aactgcaggg
      601 tctgcgcgtc gcgcgcgccg tcgcgcggct gaatcagacg cagggggtgc tgccatgagc
      661 cggcaggact ggcggcagca cctgccgcgc acaccatccc tgctgacatc cgtgctgccg
      721 gcgctggccc tgctcgtgct gaccctcgcc gctccggcgc cggtgtccgc gcacggcggc
      781 gaggaccaca gccacgaggc cgctgccgcc gccccggcgc tgccggtggc gctggccccg
      841 cgcttcgagg cgacgagtcc ggccttccag ctgctcggca tcctcgagaa cggccggctg
      901 acgctgtatc tcgaccgctt cgccgacaac gcgccggtcg ccggcgcgac gctgacggtc
      961 gaggtcgatg gccgcacgca gcccgccccc gaaaccggcg cggccaccta cgcgctcgcc
     1021 gagaccggtg cgctcgcgaa ccccggcacg cacgagctgc tggtcagcgt cacggccggc
     1081 gacgagcagg acgtgctgat cggcacgctg agcgtgccgc cggccgcggc gtccggagcg
     1141 gcggccccgg ccgctgccgg cggcatcggc ggcatcggcg gccccggctg ggggctggct
     1201 ggactggcgc tgctgctcgg ctacggcctg ggccggctcg gacgccgcac tgcaccggca
     1261 tcgccgctgc tgatcgccgg tgcgctcggc ctcgggctcg cgctcgcggc gctgccgccg
     1321 gcggcgcacg cgcacggcgg cgaggatcac ggtcacgacg aggccgcgcc gctgccggcg
     1381 acgctgaccg gcgatgcgcc gcggcggctg cccgatggca gcgtgttcgt gccgaagccg
     1441 gtcgggcgcc tgctcgggct gcgcacgcag ctcgcggcga tggcgacgct cgcgcagacc
     1501 gtggtcgtgc cggggcatct gatcgccgat ccgaaccgca gcggccgcgt gcaggcaccg
     1561 caggccgggc gcatcgtgcc ggcggccggc ggcatcgcgc acctcggcca gccggtgcgc
     1621 gccggcgagg tgctggcctg gctggagccg ctggcggcga gcctcgaagc tgccggccag
     1681 agcgccggac tcgccgagct cgacagccag atcgaactgg ccacgcagcg cctgacgcgc
     1741 ctgcagcagc tcgccggcag cgtcgcgcag cgcgagatcg acgaggcgcg caccgagctt
     1801 gccggcctgc gcaagcgccg ttcggcgctc ggcaccggac tcggcgggcg cgagccgctg
     1861 cgcgcgccgg tcgatggcgt cgtcagccgc agcacgctgg tcgccggcca gatcgtcgag
     1921 gcgcgcgaga cgctggtcga gatcctcgac cccggcgcct ggtgggtcga ggccgtggtg
     1981 ttcgatgccg gcctcgccgg caacatcgac gccgcactcg ccgaaaccga caacggccgc
     2041 acgtaccggc tcggcttcct cggcatgggc ttcgagcggc gcgcccaggg cctgccgatg
     2101 cagtggcgcg tcgacgggga cggccgcggc ctcagcgccg gccagccggt gcggctgctg
     2161 ctgcagacgc gcagccgcag ccgcggcgtg ccgctgccgg tcgccgccct cgtgcgcggg
     2221 gccgatggcg agtcgcgcgt ctgggtgcag gccggcatcg agcgcttcgt gccgcggcgc
     2281 gtgcaggtcc agccgctcga cggcgagcgc gtgctggtca ccggggggct ggccgacggc
     2341 gagcgcttcc tggtcattgg cgcgagcctg ctcgcgcagc tgcgctgaag cggagggcgc
     2401 cgtcatgttc gatcgcctga tccgcaccag cctggcgctg cgcctgccga cgctggtcgc
     2461 cgcgctggtg ctgctggtct ggggcgtgca gctcgcacgc gaactgccgg tcgatgtgtt
     2521 cccggacctc aaccgcccga ccgtgacgct gatgaccgaa gccgagggca tggcgcccga
     2581 ggaggtcgag agcctggtca cggtgccgct cgaaaccgcg atgaacggcc tggccggcgt
     2641 gcgccgcgtg cgttcggtgt ccggcgtcgg cctgtcggtg ctctacgtcg agttcgactg
     2701 gggcaccgac gtgttccgca accgccagca ggtcgccgag cgcatcggca gcgtgcaggc
     2761 gcagctgccg ccgggcatcg tgccgcagat ggggccggtc acgtcgatca tgggcgagat
     2821 catgctgatc gcgctgaagg ccggcgacgg cagcggcgac gggcacggcg gcgaagccgt
     2881 gacgccgatg cagctgcgcg atctggccga ctggacgctg cgcccgcagt tgctcgccat
     2941 cgccggcgtt gcgcaggcga tcccgatcgg cggcgaggtg cgccagatcc aggtcgtgcc
     3001 cgacccggcg ctgatggagg acctcgacgt gacgccgcag cagctcgtcg aggcgctgca
     3061 gggcttcggc gccaacgtca gcggcggctt cctcgatcac ggcgggcgcg aggtgctgat
     3121 ccgcgcgctc gggcgcagcg cccgcatcgc cgatctcgaa gggctggtgg tcgcggtgcg
     3181 cgacgaccgg ccggtgtacc tcaagcagct cgccagcgtg cgcgtcgccg cgcgcaccaa
     3241 gcgcggcgac gccagcgtcg acggccggcc cggcgtcatc gtctcggtgc agaagcagcc
     3301 gagcgccgac accgtggacc tgagccggcg catcgacgcg acgctcgcga atctcgcgcg
     3361 cacgctgccg cccggcgtgc aggtcgaggt gctgttccgg caggcgaact tcatctcggc
     3421 cgcggtcggc aacgtcgagg atgcgctgcg cgacggtgcg atcatggtcg cgatcgtgct
     3481 gttcctgttc ctgctcaacg tgcgcacgac gctgatctcg ctgaccgcga tcccgctgtc
     3541 gctgctggtg gcggtgctgg tgttcgaggc gttcgggctt tcgatcaaca cgatgacgct
     3601 cggcggactc gcgatcgcga tcggcggcct ggtcgacgat gccgtggtcg gcgtcgagaa
     3661 cgtgctgcgg cggctg
//
LOCUS       DAOQJH010000224         3659 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_15904_length_3659_cov_3.917314, whole genome shotgun sequence.
ACCESSION   DAOQJH010000224 DAOQJH010000000
VERSION     DAOQJH010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3659)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3659)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3659
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15904_length_3659_cov_3.917314"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..449
                     /locus_tag="PLQ18_06245"
     CDS             <1..449
                     /locus_tag="PLQ18_06245"
                     /inference="COORDINATES: protein motif:HMM:NF020680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit delta' C-terminal
                     domain-containing protein"
                     /protein_id="HNE01289.1"
                     /translation="PRCGEADAALLLALAAGSPLRALALAGALERRRELFRAFLDLAA
                     GKLDAVRAAERFLAGGAPAEVAGWLAGWHHDLLRLTMAAGSAPLTNPDYAAPLATLAA
                     RVAPQWLVLRWDAVLALRARLATPLNAQLAFEDFFIDWAGAAARRG"
     gene            460..828
                     /locus_tag="PLQ18_06250"
     CDS             460..828
                     /locus_tag="PLQ18_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007641447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNE01290.1"
                     /translation="MEPTRGNAAAASKQGVMSIALRDRNQLLQCFMPFLKHGGLFIPT
                     DKPFELGDDVFLLLTLMDEPTRYPVTGKVVWLTPKTAIGGRPAGIGVQFAGMEAPTVL
                     KKIETYLAGILTSDRTTYTL"
     gene            875..1654
                     /locus_tag="PLQ18_06255"
     CDS             875..1654
                     /locus_tag="PLQ18_06255"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007194675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HNE01291.1"
                     /translation="MLVDSHCHLDRVDLRPFGGELAGVLDAAAAAGVGHLLCVGIALE
                     TWPAMVEQVAPFANVSLSVGVHPSEDGREPQVAELVRLARTTARVVAIGETGLDYHYG
                     AERGDLQRAWFRTHVRAARETGKPLIVHTREARADTLAILAEEGAAEVGGVLHCFTED
                     WDMAARAVDLNFHVSFSGIVTFKSAAQIQEVARRLPAERMLVETDSPYLAPVPHRGRS
                     NHPAWVREVAAFVAKLRGEPFEQLAATTTGNFFRLFRDAVR"
     gene            1747..2382
                     /locus_tag="PLQ18_06260"
     CDS             1747..2382
                     /locus_tag="PLQ18_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019646849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HNE01292.1"
                     /translation="MTADTNAGISGSGLLARIRTRLALAPDPASLGGDHAVAGLPRPE
                     RVLTPAAVLVPIVLRADGPSVLLTQRTEHLLNHPGQISFPGGRSEAEDAGPVDTALRE
                     AEEEIGLARRHVEVVGFLDLYQTVTGFLVTPVVAFVEPGFALSLDAFEVAEAFEVPLA
                     FVLDAGNHTRGSRLVDGRERQFYVLPYQDRYIWGATAAMLVNLAHKLAPDD"
     gene            complement(2396..3355)
                     /locus_tag="PLQ18_06265"
     CDS             complement(2396..3355)
                     /locus_tag="PLQ18_06265"
                     /inference="COORDINATES: protein motif:HMM:NF024748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HNE01293.1"
                     /translation="MSEPDPIRRRLLAAVAALPVLLAPLPAAAAGSSKPQAARGAAPR
                     RSARPAPSARPPRTPAPLLNTLVGARIEALRGRGLLDRSETVAFYVQDLLSGRVLLSI
                     NGSAAMQAASMVKPYVAAAFFAEVQAGRLRYDRSQQALMEAMIVHSDNAATNHFIRAL
                     GGPARVVAALRRVAPGIASGANINEYIPAGGRTYRNRIACTTHARFLGELWSLRLPGA
                     RELLRVMGLPNRDRICSGAPGIPPGTGIHDKTGTTALLCGDMGIVVARTQAGGQWPYA
                     FIGIVQRRTPSASYGRFMHTRGDAIRDISGLVYRELRPHYGLA"
     gene            3543..>3659
                     /locus_tag="PLQ18_06270"
     CDS             3543..>3659
                     /locus_tag="PLQ18_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005805246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HNE01294.1"
                     /translation="MNDFLDEMNRRRTFAIISHPDAGKTTLTEKLLLFGGAIQ"
BASE COUNT          489 a         1317 c         1327 g          526 t
ORIGIN      
        1 cgccgcgctg cggcgaggcc gatgcggcgc tgctgctcgc gctcgccgcc ggcagcccgc
       61 tgcgggcgct ggcgctcgcc ggcgcgctgg agcggcggcg cgagctgttc cgggccttcc
      121 tcgatctggc cgccggcaag ctcgatgcgg tgcgcgccgc cgagcgcttc ctcgccggcg
      181 gcgcaccggc cgaggtcgcc ggctggctgg ccggctggca ccatgatctg ctgcgcctta
      241 ccatggccgc cggcagcgcg ccgctgacga accccgacta cgcggccccg ctcgcgacgc
      301 tggccgcccg cgtcgcgccg cagtggctgg tcctgcgctg ggacgcggtg ctcgcgctgc
      361 gcgcgcggct tgccacgccg ctgaacgcgc agctcgcgtt cgaggacttc ttcatcgact
      421 gggccggcgc cgccgcccgc aggggctgac ccgatgggaa tggaaccgac acgcggcaat
      481 gccgcggccg cctccaagca gggcgtgatg tcgatcgcgc tgcgcgaccg caaccagctg
      541 ctgcagtgct tcatgccgtt cctgaagcac ggcggcctgt tcattccgac cgacaagccc
      601 ttcgaactcg gcgacgatgt cttcctgctg ctgaccctca tggacgagcc gacgcgctat
      661 ccggtgaccg gcaaggtcgt ctggctgacg ccgaagaccg cgatcggcgg ccggccggcc
      721 ggcatcggcg tgcagttcgc cggcatggag gcgccgaccg tgctgaagaa gatcgagacc
      781 tacctggccg gcatcctgac ttcggaccgc accacctaca cgctgtgatc gctgacgggc
      841 acccgcggcc cggcagcggc gccggagacc gcacatgctc gtcgattccc actgtcacct
      901 cgatcgcgtc gacctgcgcc cgttcggcgg cgaactggcc ggcgtgctcg atgccgccgc
      961 tgcggccggc gtcgggcacc tgctctgcgt cggcatcgcg ctggagacct ggccggcgat
     1021 ggtcgagcag gtcgcaccgt ttgcgaacgt ctcgctgtcg gtcggcgtac acccgagcga
     1081 ggacgggcgc gagccgcagg tcgccgaact cgtgcggctg gcgcgcacca cggcgcgcgt
     1141 ggtcgccatc ggcgagaccg gcctcgacta ccactacggc gccgaacgcg gcgatctgca
     1201 gcgcgcgtgg ttccgcaccc acgtgcgcgc cgcccgcgag accggcaagc cgctgatcgt
     1261 gcacacgcgc gaggcgcgcg ccgacacgct ggcgatcctc gccgaagagg gcgcggccga
     1321 ggtcggcggc gtgctgcatt gtttcaccga ggactgggac atggcggcgc gcgccgtcga
     1381 cctgaacttc catgtgtcgt tctcgggcat cgtcaccttt aaaagcgccg cgcagattca
     1441 ggaagtcgcg cgccggctgc ccgccgaacg catgctggtc gagaccgact cgccgtatct
     1501 ggcaccggtg ccgcaccgcg gccgcagcaa tcacccggcc tgggtgcgcg aggtcgcggc
     1561 cttcgtcgca aagctgcgcg gcgaaccctt cgagcagctg gcggcgacga cgaccggcaa
     1621 ttttttcagg ctgtttcgcg atgccgtgcg ctgaacgccc ggcgctgtca tgaagcggta
     1681 aactccatgc tcgctgccgc ggttctgacg cgcattgacg gagagccccg cttcatgatt
     1741 gccgagatga ccgctgacac gaacgccgga atttccggct ccgggctgct cgcgcgcatc
     1801 cgcacgcggc tggcgcttgc ccctgatccg gcgagcctcg gcggcgacca cgcggtcgcc
     1861 ggcctgccgc gccccgagcg cgtgctgacg ccggcggcgg tgctggtgcc gatcgtgctg
     1921 cgcgccgacg ggccgagcgt gctgctgacg cagcgcaccg agcacctgct gaaccacccc
     1981 ggccagatca gcttcccggg cggacgcagc gaagccgagg atgccgggcc ggtggacacg
     2041 gcgctgcgcg aggccgagga agagatcggt ctggcgcgcc ggcacgtcga ggtggtcggc
     2101 ttcctcgacc tgtaccagac cgtcaccggc tttctggtga cgccggtcgt cgccttcgtc
     2161 gagccgggct tcgcgctgag cctcgacgcc ttcgaggtcg ccgaggcttt cgaggtgccg
     2221 ctcgccttcg tgctcgacgc cggcaaccac acgcgcggca gccgcctcgt cgatggccgc
     2281 gagcggcagt tctacgtgct gccctaccag gatcgctaca tctggggcgc gaccgcggcg
     2341 atgctcgtca acctcgcgca caagctcgcc ccggacgact gatccggggc cggcttcagg
     2401 ccagcccgta gtgcgggcgc agctcgcggt agaccaggcc cgagatgtcg cggatcgcgt
     2461 cgccgcgcgt gtgcatgaag cgtccgtagc tcgcactcgg cgtgcgccgc tgcacgatgc
     2521 cgatgaacgc gtacggccac tggccgccgg cctgcgtgcg cgcgacgacg atgcccatgt
     2581 caccgcagag cagcgccgtc gtgccggtct tgtcgtggat gccggtgccg ggcgggatgc
     2641 cgggtgcgcc gctgcagatg cggtcacgat tcggcaggcc catcacgcgc agcagctcgc
     2701 gcgcgcccgg cagacgcagg ctccagagct cgccgaggaa gcgcgcgtgc gtggtgcagg
     2761 cgatgcggtt gcggtaggtg cggccgccgg ccgggatgta ctcgttgatg ttcgcaccgc
     2821 tggcgatgcc gggcgcgacg cggcgcagcg ccgcgaccac gcgcgccggc ccgccgagcg
     2881 cgcggatgaa gtgattggtc gcggcgttgt cgctgtgcac gatcatcgct tccatcagcg
     2941 cctgctgcga gcggtcgtag cgcagccgcc cggcctgcac ctcggcgaag aacgcggcgg
     3001 cgacgtaggg cttgaccatg ctcgccgcct gcatcgccgc cgagccgttg atcgacagca
     3061 gcacgcggcc ggacagcagg tcctgcacgt aaaaggcgac cgtctcgctg cgatcgagca
     3121 ggccgcggcc gcgcaaggcc tcgatgcggg cgccgaccag cgtgttcagc agcggtgccg
     3181 gcgtacgcgg cggccgggcg gacggtgcag ggcgggcgct gcggcgtggc gcggcacctc
     3241 ttgcggcttg cggcttgctg ctgccggcgg ctgcggccgg cagcggggcg agcagcacgg
     3301 gcagggccgc aacggccgcg agcaggcggc ggcggatcgg atcaggctcg gacatggcgg
     3361 gacgggtgtg cggcggaatg accccggcag tatggccaga tgctccggca gcgtcacccc
     3421 ggatgcggcg gtcgccggtg ccgggccggc ggggctgcgg tagactccgc ggtctttttc
     3481 ggcgccggac gggctgtccg ccggcatcca tggtccccga acgacgcaca gcgcagggcg
     3541 cgatgaacga cttcctcgac gagatgaacc ggcggcgcac cttcgcgatc atctcccacc
     3601 ccgacgccgg caagaccacg ctgaccgaga agctgctgct gttcggcggc gcgatccag
//
LOCUS       DAOQJH010000225         3652 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_15955_length_3652_cov_4.312483, whole genome shotgun sequence.
ACCESSION   DAOQJH010000225 DAOQJH010000000
VERSION     DAOQJH010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3652)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3652)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3652
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15955_length_3652_cov_4.312483"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..846)
                     /locus_tag="PLQ18_06275"
     CDS             complement(<1..846)
                     /locus_tag="PLQ18_06275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE01295.1"
                     /translation="MSKRTRQFGRGCLSLFVLVSGTAAAGDVTMQSSLSVSGTLTDNI
                     TRGQIQSDGSPVSKGDFITEIRPSIGGRSQGAGLKYDFNYELSAVEYFHDTARNAVNN
                     RFRLNGTAEVVEDRFLLDFRSAYFQVANAPSADTLNDPLLGSGGYSNVGTLTLTPIWR
                     EHLGSFADSEMTGTLGLTTTSDQSDNVGGASASASDSTVLGIDWRLSNGRSSGNTGWS
                     VVYSRSVESYADGSLSNDEREHAEASVRQGISREWALLGRVGWENNSTHDISDQEAAN
                     GAYWSG"
     gene            complement(839..1765)
                     /locus_tag="PLQ18_06280"
     CDS             complement(839..1765)
                     /locus_tag="PLQ18_06280"
                     /inference="COORDINATES: protein motif:HMM:TIGR03018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XrtA-associated tyrosine autokinase"
                     /protein_id="HNE01296.1"
                     /translation="MDTIEKAIERLSRHERQPAPVAEAQGTAAIVIEPAPLPAVPGSQ
                     PVRAETPAAPAVVRRTKRSVTLDLQRIRDAGMVVPDGNRSRIKEEYRHIKRPLLLNAA
                     GKGAAVRPNQNVMMVSSSQPGEGKTFTSINLALSIAAERDRTVLLVDADVLKPTVSRF
                     FGVDNGPGLVDFLVDGQLDLSEVLVETNVPSLVLLPAGNSHHLSTELLGSTAMRDLMT
                     EMSNRYSDRVIIMDSPPLLATTEAAVLAHLVGQIIMVVEAERTRQSLVKEALALLDPD
                     MAVGFVLNKSRQAARGDYYGPYYYSYAEPARE"
     gene            complement(1787..3385)
                     /locus_tag="PLQ18_06285"
     CDS             complement(1787..3385)
                     /locus_tag="PLQ18_06285"
                     /inference="COORDINATES: protein motif:HMM:TIGR03007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chain-length determining protein"
                     /protein_id="HNE01297.1"
                     /translation="MHEIVEQILGYLRGMWRYRWYALAVAWTISLGGWFFVHRLPDQF
                     EASARVYVDTQSILRPLLRGLTVDVNPDSQIQLMTRTLLSRPNLEKVARMTDLDLKAR
                     DNAQMDVLLDRLGKHISLRSLGRENLYTIGFEDKDPGFAKKVVQSLLTIFVENALGET
                     RQDSDTAQRFLDKQIAEYEARLLAAEERLKNFKRQNVDMMPQSGQDHYSKMQEMGSQL
                     AAARLELKQIEQRRDTLKAQLDGDTATFGVGPKPVIAQEGPASITDSAPVDGRIKMME
                     ERLDQLLLQYTEKHPDVIAVRQTIADLRKQREKEIAEIRAATAAAAKAASSNGGEPVQ
                     DGNPYLQQVRMMLTAEEGNAAASRVRVEEFERRLAEMQRMVNSVPEVEAQLKALNRDY
                     DVTRQNYDQLLARRESARLSQQAGQTNEDIKFKVIDPPRVPNEPTGPNRVGLMSGVFG
                     GAVIGGIAVAFLISQLRPTFDSRRMLTDLTGLPVLGMVSLVVSPDTIRRQRYGLAGFV
                     GMGMLLVLTFGGLLAAQLTGHRLI"
     gene            complement(3428..>3652)
                     /locus_tag="PLQ18_06290"
     CDS             complement(3428..>3652)
                     /locus_tag="PLQ18_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014977096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HNE01298.1"
                     /translation="SIPYREKMSLLDVMILVGGLTQFADGNEAKIVRVVEGQQQQYRV
                     RIDDLLKDGDITANVDMLPGDILIVPESWL"
BASE COUNT          548 a         1191 c         1261 g          652 t
ORIGIN      
        1 accgctccag taagcgccgt tggcggcttc ctggtcgctg atgtcatggg tcgagttgtt
       61 ttcccagccg acccgcccca gcagtgccca ttcgcggctg atgccctggc gcacgctcgc
      121 ttcggcgtgc tcccgctcgt cgttgctgag gctgccatcg gcgtaacttt cgaccgaacg
      181 cgaatagacg acgctccagc cggtattgcc gctgctgcga ccgttgctga gccgccagtc
      241 gatgccgagc acggtcgaat ccgaagcgct ggccgaggcg ccgccgacgt tgtcgctctg
      301 gtcggaggtc gtcgtcagac cgagcgtgcc ggtcatttcg gagtcggcga agctgccgag
      361 gtgctcgcgc cagatcggcg tcagcgtcag cgtgccgacg ttcgaatacc cgcccgaacc
      421 gagcagcgga tcgttcaggg tatcggccga cggcgcattg gcgacctgga agtacgcgga
      481 gcggaaatcg agcaggaagc ggtcctcgac gacttcggcc gtgccgttga gccggaagcg
      541 gttgttgacc gcattgcggg ccgtgtcgtg gaaatactcc acggccgaca gctcgtagtt
      601 gaaatcgtac ttgaggccgg cgccctggct gcgcccgccg atcgaaggcc ggatctcggt
      661 gatgaagtcg cccttcgaga ccgggctgcc atccgactgg atctggccgc gcgtgatgtt
      721 gtccgtcagc gtgccgctga cgctcaggct ggactgcatc gtcacgtcgc cggcggcggc
      781 ggtccccgat accagcacga acagggacag gcagccccgg ccgaactgcc gggtccgctt
      841 actcacgcgc cggctccgcg tagctgtagt agtacgggcc gtagtagtcg ccgcgggcgg
      901 cctggcgcga cttgttcagc acgaagccga ccgccatgtc cggatcaagc agcgccagcg
      961 cctccttgac cagcgactga cgcgtgcgct cggcctcgac gaccatgatg atctggccga
     1021 ccagatgcgc gagcaccgcg gcttcggtgg tggcgagcag cggcggcgaa tccatgatga
     1081 ttacgcggtc cgaatagcgg ttgctcatct cggtcatcag gtcgcgcatc gccgtgctgc
     1141 cgagcagctc ggtcgacaga tggtgcgagt tgccggccgg cagcagcacc agcgacggca
     1201 cgttggtctc gaccaggact tcggacagat cgagctgacc atcgaccaga aagtcgacca
     1261 ggcccgggcc gttgtcgacg ccgaagaagc gcgacaccgt cggcttcagc acgtcggcat
     1321 cgacgagcag cacggtgcgg tcgcgctcgg cggcgatgct gagcgccagg ttgatcgacg
     1381 tgaaggtctt gccctcgccc ggctgcgagc tcgacaccat catgacgttc tggttggggc
     1441 gcaccgccgc gcccttgccg gcggcgttga gcagcagcgg ccgcttgatg tgccggtatt
     1501 cctccttgat gcggctgcgg ttgccgtccg gaacgaccat gccggcatcg cggatgcgct
     1561 gcaggtcgag cgtgaccgaa cgcttggtgc gccggaccac ggccggtgca gccggggttt
     1621 cggcacgcac cggctggctg ccgggcactg ccggcagcgg ggccggctcg atgacgatgg
     1681 ccgcggtgcc ctgcgcctcg gcgacgggag ccggctgccg ttcgtggcgg ctcaggcgtt
     1741 cgatggcttt ttcgatggtg tccaagaacg agcgctccag gcggcgtcag atcagccggt
     1801 ggccggtcag ctgggcggcc agcagtccgc cgaaggtcag caccagcagc atgcccatgc
     1861 cgacgaaacc cgcgagcccg tagcgctggc gccggatcgt gtcgggcgag acgaccaggc
     1921 tgaccatgcc gagcaccggc aggccggtca gatcggtcag catgcgccgg ctgtcgaagg
     1981 tcggacgcag ctgcgagatc aggaaggcca cggcgatgcc gccgatgacc gcgccgccga
     2041 acacgcccga catcagcccg acgcggttcg gaccggtcgg ctcgttcggc acgcgcggcg
     2101 gatcgatgac cttgaacttg atgtcctcgt tggtctggcc ggcctgctgc gacaggcgcg
     2161 ccgattcgcg gcgggcgagc agctgatcgt agttctggcg ggtcacgtcg taatcgcggt
     2221 tcagcgcctt cagctgcgcc tcgacctcgg ggacgctgtt gaccatgcgc tgcatctcgg
     2281 ccaggcggcg ctcgaactcc tcgacgcgca cgcgcgaggc ggcggcattg ccctcctcgg
     2341 cggtcagcat catgcgcacc tgctgcaggt agggattgcc gtcctggacc ggctcgccgc
     2401 cgttgctgct ggccgccttg gccgcggccg cggtggcggc acgaatctcg gcgatttcct
     2461 tctcgcgctg cttgcgcagg tcggcgatgg tctggcgcac ggcgatgacg tcgggatgct
     2521 tctcggtgta ctgcagcagc agctggtcga gtcgctcctc catcatcttg atgcggccgt
     2581 cgaccggtgc cgaatcggtg atgctggccg ggccttcctg cgcgatcacc ggcttcggac
     2641 cgacgccgaa ggtggccgtg tcaccgtcga gctgcgcctt cagcgtgtcg cgccgctgct
     2701 cgatctgctt cagttcgagg cgtgcggccg cgagctgcga gcccatctcc tgcatcttgc
     2761 tgtaatggtc ctgaccggac tgcggcatca tgtcgacgtt ctggcgcttg aagttcttca
     2821 ggcgctcctc ggccgcgagc aggcgtgcct cgtactcggc gatctgcttg tcgaggaagc
     2881 gctgcgcggt gtcggagtcc tgccgggtct cgccgagcgc gttctcgacg aagatcgtca
     2941 gcagcgactg cacgaccttc ttcgcgaagc ccggatcctt gtcctcgaaa ccgatcgtgt
     3001 agaggttctc gcgcccgagg ctgcgcagcg agatgtgctt gccgaggcga tcgagcagca
     3061 cgtccatctg cgcgttgtcg cgggccttca ggtcgaggtc ggtcatgcgc gcgaccttct
     3121 cgaggttcgg ccggctgagc agcgtgcgcg tcatcagctg gatctgcgaa tccgggttca
     3181 cgtcgacggt caggccgcgc agcagcgggc gcaggatcga ctgcgtgtcg acgtagacac
     3241 gcgccgaggc ctcgaactgg tcgggcaggc ggtgcacgaa gaaccagccg ccgaggctga
     3301 tggtccaggc gacggccagc gcgtaccagc ggtaacgcca catgccgcgc aggtaaccga
     3361 ggatttgttc gacgatctcg tgcatggcgg aaaccgtggt tcttcgtggt tcttcgtgga
     3421 actgcgctta cagccaggat tccggaacga tcaggatgtc gcccggcagc atgtcgacgt
     3481 tggcggtgat gtcgccgtcc ttcagcaggt cgtcgatgcg cacgcggtac tgctgctgct
     3541 gaccctcgac gacgcgcacg atcttggcct cgttgccatc ggcgaactgc gtcaggccgc
     3601 cgacgaggat catcacgtcg agcagcgaca tcttctcgcg gtacgggatc ga
//
LOCUS       DAOQJH010000226         3648 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_15993_length_3648_cov_3.386307, whole genome shotgun sequence.
ACCESSION   DAOQJH010000226 DAOQJH010000000
VERSION     DAOQJH010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3648)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3648)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3648
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15993_length_3648_cov_3.386307"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            75..716
                     /gene="hisG"
                     /locus_tag="PLQ18_06295"
     CDS             75..716
                     /gene="hisG"
                     /locus_tag="PLQ18_06295"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018954265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HNE01299.1"
                     /translation="MSDTLTIALSKGRIFQETLPLLAAAGIRPLEDPETSRKLILDTD
                     RPDVKLVIIRATDVPTYVQYGAADLGVAGKDVLMEHGGEGLYEPLDLGIARCRLMTAG
                     RPGVPEPRGRMRVATKFANVARRFYAQQGRQVEIIKLYGSMELAPLVGLADRIVDIVD
                     TGKTLRANGLEPLELIADISSRLVVNRAAMKMKHARVRTILEHMAAATGRAVA"
     gene            791..2113
                     /gene="hisD"
                     /locus_tag="PLQ18_06300"
     CDS             791..2113
                     /gene="hisD"
                     /locus_tag="PLQ18_06300"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HNE01300.1"
                     /translation="MDIARLNYSDPDFWPRLDRLTAWEGVSDAHVAGTVAQIIADLRA
                     RGDAALLELTNRYDRTAFAAAAELEIPAERLDAALCALPPAQRTALETAAARVRAYAE
                     RQKLDSWSYTEADGTLLGQQVTALDAVGLYVPGGKAAYPSSVLMNAIPAKVAGVRQLV
                     MVVPTPDGEQNDLVLAAARLAGVDRVFAIGGAQAVAALAYGTASVPRVDKIVGPGNIW
                     VATAKRAVYGQVGIDMIAGPSEILVICDGDTDADWIAMDLFSQAEHDEDAQPILISPD
                     AAFLDRVAAAIERLLPTLARADIVRVSLASRAALIRVGDLAEAAEVANYIAPEHLELS
                     VADPQALLPSIRHAGAIFLGRHTAEALGDYCAGPNHVLPTSRTARFSSPLGVYDFQKR
                     SSLIFCSPDGAATLGRTASVLARGEGLSAHARSAEYRIPGFVPPEAAS"
     gene            2110..3168
                     /gene="hisC"
                     /locus_tag="PLQ18_06305"
     CDS             2110..3168
                     /gene="hisC"
                     /locus_tag="PLQ18_06305"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015475843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HNE01301.1"
                     /translation="MSRYWSEVVGRLTPYVPGEQPQIPNLVKLNTNEHPDGPSPRVLA
                     AIRAALDEGLRLYPDPNAGRLKQALAARHGLTAAHVFVGNGSDEVLAHAFHGLLRHER
                     PLLFPDVTYSFYPVWCGLYDIGFETIPLAGDFSLRVEDYRRPNGGIVFANPNAPTGRA
                     LALADIERLLGFNRDSVVLVDEAYVDFGADSAAALVAQYPNLLVVQTLSKSRALAGLR
                     VGWAYGDPALIEALERIKNSFNSYPLDRLALAGAVAALEDDEYFDVHRRAVIDSREQL
                     AQALTDHGFEVLPSAANFVFARHPAHDAAGLAAGLRAHGVIVRHFRLPRIEQFLRITV
                     GSPAQNAALLAALAKLHG"
     gene            3180..>3648
                     /locus_tag="PLQ18_06310"
     CDS             3180..>3648
                     /locus_tag="PLQ18_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012699942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HNE01302.1"
                     /translation="MHLRELVGYCDTLLAAASFSDYCPNGLQVEGRPDVRLLVGGVTA
                     CQALLEAALDLGADAILVHHGYFWRGEDARVTGQKRRRLATLLRHDLSLIAYHLPLDA
                     HAEYGNNVALARTLGLRISGALDGGGRTPGLVLHGEPAGSAGAVCVVGQVQPLV"
BASE COUNT          502 a         1369 c         1247 g          530 t
ORIGIN      
        1 gaggagaagc tcggcagtct cggcgcgctg atccgccgcg tgccctgagc cccccggagt
       61 cagagcttcc cgccatgtcc gacacgctga ccatcgcgct ctcgaaggga cgcatcttcc
      121 aggagacgct gccgctgctc gccgcggccg gcatccggcc gctcgaagac ccggagacca
      181 gccgcaagct gatcctcgac accgaccgcc cggatgtgaa gctcgtcatc atccgcgcca
      241 ccgacgtgcc gacctacgtg cagtacggtg cggccgacct cggcgtcgcc ggcaaggacg
      301 tgctgatgga gcacggcggc gaggggctct acgagccgct cgatctcggc atcgcccgct
      361 gccggctgat gaccgccggc cggccgggcg tgccggagcc gcgcggacgc atgcgcgtcg
      421 cgaccaagtt cgccaacgtc gcgcgccgct tctatgcgca gcagggccgg caggtcgaga
      481 tcatcaagct gtacggctcg atggaactcg cgccgctggt cggccttgcc gatcgcatcg
      541 tcgacatcgt cgataccggc aagacgctgc gcgccaacgg tctggagccg ctggagctga
      601 tcgccgacat cagctcgcgg ctggtcgtca accgcgcggc gatgaagatg aagcacgcgc
      661 gcgtgcgcac gatcctcgaa cacatggccg ctgcgaccgg tcgcgccgtc gcctgaggcc
      721 ggccgggctg ccggccctat ccgtcccctc cgtccttgcg cacgcgcttg ccgtgcagga
      781 gccccccgcc gtggacatcg cccgcctgaa ctacagcgat ccggacttct ggccgcgtct
      841 cgaccggctg acggcctggg agggcgtctc cgatgcccac gtcgccggca cggtcgcgca
      901 gatcatcgcc gacctgcgtg cgcgcggcga cgcggccctg ctcgaactga ccaaccgcta
      961 cgaccgcacg gccttcgccg ctgccgccga gctggaaatc ccggccgagc gcctcgatgc
     1021 cgcactctgc gcgctgccgc cggcgcagcg cacggcgctc gaaaccgcgg cggcgcgcgt
     1081 gcgtgcctac gccgagcgcc agaagctcga ttcctggagc tacaccgagg ccgacggcac
     1141 gctgctcggc cagcaggtca cggcgctcga tgcggtcggc ctgtacgtgc cgggcggcaa
     1201 ggccgcctat ccgtcctcgg tgctgatgaa cgcgatcccg gccaaggtcg ccggcgtcag
     1261 gcagctggtg atggtcgtgc cgacgccgga cggcgagcag aacgatctgg tgctcgccgc
     1321 ggcgcgcctc gccggcgtcg atcgcgtgtt cgcgatcggc ggtgcgcagg cggtcgccgc
     1381 gctcgcctac ggcacggcga gcgtgccgcg cgtcgacaag atcgtcggcc ccggcaacat
     1441 ctgggtcgcg accgccaagc gcgccgtgta cgggcaggtc ggcatcgaca tgatcgccgg
     1501 accgtccgag atcctcgtga tctgcgacgg cgacaccgat gccgactgga tcgcgatgga
     1561 tctgttctcg caggccgagc acgacgagga cgcgcagccg atcctgatct cgccggatgc
     1621 ggccttcctc gatcgcgtcg ccgccgccat cgagcgcctg ctgccgacgc tggcgcgcgc
     1681 cgacatcgtg cgcgtgtcgc tggccagccg cgcggcgctg atccgggtcg gcgaccttgc
     1741 cgaggcggcc gaagtcgcca actacatcgc gcccgagcac ctggagctgt cggtcgccga
     1801 tccgcaggca ttgctgccga gcatccgcca cgccggcgcg atcttcctcg gccggcacac
     1861 ggccgaggcg ctcggcgact actgcgccgg gccgaaccac gtgctgccga cctcgcgcac
     1921 cgcgcgcttc tcgtcgccgc tcggtgtcta tgacttccag aagcgttcga gcctgatctt
     1981 ctgctcgccg gacggggccg ccacgctcgg gcgcacggcc tcggtgctgg cgcgcggcga
     2041 ggggctgagt gcgcacgcgc gctcggccga gtaccgcatc cccggcttcg tgccgccgga
     2101 ggccgcgtcg tgagccgcta ctggagcgag gtcgtcggcc ggctgacgcc gtacgtgccc
     2161 ggcgagcagc cgcagatacc gaacctggtc aagctcaaca ccaacgagca cccggacgga
     2221 ccctcgccgc gcgtgctggc agcgatccgc gccgccctcg acgagggcct gcgcctgtat
     2281 ccggacccga acgcgggccg gctgaagcag gcgctggccg cacgccacgg actcacggcc
     2341 gcgcacgtgt tcgtcggcaa cggttccgac gaggtgctcg cgcatgcctt ccacggcctg
     2401 ctgcgtcacg aacggccgct gctgttcccg gacgtgacct acagcttcta cccggtctgg
     2461 tgcgggctgt acgacatcgg cttcgagacc atcccgctcg ccggcgactt ctcgctgcgc
     2521 gtcgaggact accgccggcc gaacggcggc atcgtgttcg cgaacccgaa cgcgccgacc
     2581 ggccgggcgc tggcgctcgc cgacatcgag cgcctgctag gcttcaaccg cgattcggtc
     2641 gtgctggtcg atgaggccta cgtcgatttc ggcgccgaca gcgccgctgc gctggtcgcg
     2701 cagtacccga acctgctggt cgtgcagacg ctgtcgaagt cgcgcgcgct cgccggcctg
     2761 cgcgtcggct gggcgtatgg cgatcccgcg ctgatcgagg cgctggagcg catcaagaac
     2821 agcttcaact cctacccgct cgaccggctg gcgctcgccg gcgccgtggc cgcgctcgag
     2881 gacgacgaat acttcgacgt gcaccggcgt gcggtcatcg acagccgcga acagctcgcg
     2941 caggcgctga cggatcacgg cttcgaggtg ctgccctcgg ctgcgaactt cgtgttcgca
     3001 cgccatccgg cgcacgatgc cgccggcctc gccgccggcc tgcgcgcgca cggcgtcatc
     3061 gtccgccact tccggctgcc gcgcatcgag cagttcctgc gcatcacggt cggcagcccc
     3121 gcgcagaacg ccgcgctgct cgcggcgctc gcgaagctgc acggctgagg cggcacgcca
     3181 tgcacctgcg cgagctggtc ggctactgcg acacgctgct cgccgcggcg agcttcagcg
     3241 actactgccc gaacggcctg caggtcgagg gccggcccga cgtgcggctg ctggtcggcg
     3301 gcgtcaccgc ctgtcaggcg ctgctcgaag cggcgctcga tctcggtgcc gatgcgatcc
     3361 tcgtgcatca cggctacttc tggcgcggcg aggatgcgcg tgtcaccggt cagaagcgcc
     3421 ggcgcctggc gacgctgctg cgccacgatc tctcgctgat cgcctaccac ctgccgctcg
     3481 atgcgcacgc cgaatacggc aacaacgtcg cgctcgcccg cacgctcggc ctgcgcatca
     3541 gcggggcgct cgacggcggc ggacgcacgc cggggctggt cctgcacggc gagccggccg
     3601 gaagcgccgg cgccgtatgc gtcgtcggtc aggtccagcc actcgtag
//
LOCUS       DAOQJH010000227         3642 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_16031_length_3642_cov_3.543909, whole genome shotgun sequence.
ACCESSION   DAOQJH010000227 DAOQJH010000000
VERSION     DAOQJH010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3642)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3642)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3642
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16031_length_3642_cov_3.543909"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1503
                     /locus_tag="PLQ18_06315"
     CDS             <1..1503
                     /locus_tag="PLQ18_06315"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNE01303.1"
                     /translation="LELSSGTAQPERLRTALAEIDDFVAGIDGFVGRLETHAEAKLTA
                     LRWVQGAILLATLLLAVLAWRSVRLRLLPPLRELLGLADRVRRGDFIGRAEHVGRDEL
                     GVLAQAFNAMAEDLSKMYADLESRVEAQTHALRRSNRSLELLYATTRRLNEAPPTAAT
                     LTAVLAEVERYTGARGSSLCLVDAQSLKLQQKISTHPDFPERCRKPDCGSCVGDGSSS
                     EPAHLPQTLSVPVQELGEYYGVLLLEAPGIGVDDATQVQLLETVARHIASTLRATRQE
                     VSKRRLALLEERNVIARELHDSLAQQLSYLKIQVSRLQSALPDGQPAAVGEILGELRE
                     GLSSAYRELRELLTTFRITMAEDGLQPALERALREFGTRGNLATVLDDRLNGHPLSPH
                     QEIHVLQIVREALSNIVHHARARTARVTLAHADGRMQVTIEDDGVGLSDNPERERHYG
                     LTIMRERTRSLHGELQLGRSTHGGTRVALDFPATHAEPPTDTSAEESLHV"
     gene            1496..2155
                     /gene="narL"
                     /locus_tag="PLQ18_06320"
     CDS             1496..2155
                     /gene="narL"
                     /locus_tag="PLQ18_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010626257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HNE01304.1"
                     /translation="MSDAPQTLLVIDDHPLLRKGVSQLVAMEPEMRLVGEAADGSEGL
                     KLARQLEPDLILLDLQMKGMNGLETLKAIKALDTDSRVIMFTVSDSEDDILAALQAGA
                     DGYLLKDMEPEEMLANLRMAARGRLVLSGPVTELLARSLRNESSSGALPAADLTDREH
                     EILLHIAEGRSNKLIARALDITEGTVKVHVKHLLKKLNLRSRVEAAVWAVKSGIAQRH
                     G"
     gene            2363..2638
                     /locus_tag="PLQ18_06325"
     CDS             2363..2638
                     /locus_tag="PLQ18_06325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE01305.1"
                     /translation="MSGPNRKLDRNDRYFRLPSAGSPVRLPAAVAHGAQPRLEDWCLI
                     AGDRLSGRVYDDPRFEDGEHITTSPVLLLSNHAAATCTTHYRLGRPA"
     gene            complement(2702..3553)
                     /locus_tag="PLQ18_06330"
     CDS             complement(2702..3553)
                     /locus_tag="PLQ18_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007584526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HNE01306.1"
                     /translation="MRQLSPAGQQAVDDISRRHGFSSDAVRAMLDAVINGNGTMAQFS
                     HHEFGGSGQWMAGGMLMLGDMFNHGLKSRVDALCQELSTLVANQPGLLQTGSFQSQSQ
                     GSGHNDSYQQQGNWNPPSGQSGTGSSLFTPQADWWGPELRWPNSTGAQNGVRYAYFAQ
                     AHRLAIETGGHVTIYDTLDHQIGGVSQQQGSSGTLSFTSQYGTVNLSSLPVISGGGRQ
                     PDAWSPSSSPSSPAPMNSPAPAAASGTASESDIFATIEKLAGLRAKGFISDDEFNAKK
                     AELLSRL"
BASE COUNT          599 a         1227 c         1258 g          558 t
ORIGIN      
        1 ctggaactga gcagcggtac ggcacagccc gagcgcctgc gcacggcgct cgccgagatc
       61 gacgacttcg tcgccggcat cgacggcttc gtcggccggc tggagacgca cgccgaggcc
      121 aagctgaccg cgctgcgctg ggtgcagggc gcgatcctgc tcgcgacgct gctgctggcg
      181 gtgctggcct ggcgcagcgt gcgcctgcgg ctgctgccgc cgctgcgcga actgctcggg
      241 cttgccgacc gcgtgcgccg cggcgatttc atcggtcggg cggagcacgt cggccgtgac
      301 gaactcggcg tgctggcgca ggccttcaac gcgatggccg aggatctgtc gaagatgtac
      361 gccgacctcg aatcgcgcgt cgaggcgcag acgcatgcgc tgcgccgctc caaccgttcg
      421 ctggaactgc tgtatgcgac cacgcgccgg ctcaacgagg cgccgccgac cgccgcgacg
      481 ctgaccgcgg tgctcgccga ggtcgagcgc tacaccgggg cgcgtggttc gagcctgtgc
      541 ctcgtcgatg cgcagtcgct gaagctgcag cagaagatca gcacgcatcc ggacttcccg
      601 gagcgctgcc gcaagcccga ctgcggcagt tgcgtcggcg acggcagcag cagcgaaccg
      661 gcgcatctgc cgcagacgct gtcggtgccg gtgcaggagc tcggcgagta ttacggcgtg
      721 ctgctgctcg aagctcccgg catcggcgtc gatgacgcca cgcaggtgca gctgctggaa
      781 accgtcgccc gccacatcgc ctcgacgctg cgcgccacgc gtcaggaggt ctcgaagcgg
      841 cgtctggcgc tgctggagga gcgcaacgtc atcgcccgcg agctgcacga ttccctggcc
      901 cagcagctgt cgtacctgaa gattcaggtc agccgcctgc agtcagcgct gcccgacggg
      961 cagccggctg cggtcggcga gattctcggc gagctgcgcg agggcctcag cagtgcctac
     1021 cgcgagctgc gcgagttgct gacgaccttc cgcatcacga tggccgagga cggcctgcag
     1081 ccggcgctcg aacgggcgct gcgcgagttc ggcacgcgcg gcaacctcgc caccgtgctc
     1141 gatgaccgcc tgaacggcca cccgctgtca ccgcatcagg aaatccatgt gctgcagatc
     1201 gtgcgcgagg cgctgtcgaa catcgtccat catgcgcggg cccgcaccgc gcgcgtcacg
     1261 ctcgcgcacg ccgatggtcg catgcaggtc acgatcgagg acgacggcgt cggcctgtcc
     1321 gacaatcccg agcgcgagcg tcactacggg ctgaccatca tgcgcgagcg cacgcgcagc
     1381 ctgcacggcg aactgcagct cgggcgcagc acccacggcg gcacgcgcgt ggcgctcgac
     1441 ttcccggcga cccacgccga acccccgacc gatacctccg ccgaggagtc cctccatgtc
     1501 tgatgccccg caaaccctcc tcgtcatcga tgaccacccg ctgctgcgca agggcgtatc
     1561 gcaactggtg gccatggagc cggaaatgcg cctggtcggc gaggccgccg acggcagtga
     1621 aggcctgaag ctcgcccgcc agctcgaacc cgacctgatc ctgctcgacc tgcagatgaa
     1681 gggcatgaac gggctggaga cgctgaaggc gatcaaggcg ctcgataccg attcgcgcgt
     1741 gatcatgttc acggtctcgg attccgagga tgacatcctc gccgcgctgc aggccggcgc
     1801 cgacggctat ctgctgaagg acatggagcc cgaggagatg ctcgccaacc tgcgcatggc
     1861 ggcgcgcggc cgtctggtgc tgtcggggcc ggtgaccgaa ctgctggcgc gctcgctgcg
     1921 caacgagagc agctccggcg cgctgccggc ggccgatctc accgatcgcg agcacgagat
     1981 cctgctgcac atcgccgagg ggcgcagcaa caagctgatc gcccgcgcgc tcgacatcac
     2041 cgagggcacg gtcaaggtgc acgtcaagca cctgctgaag aagctcaacc tgcgctcgcg
     2101 cgtcgaggcg gcggtctggg ccgtcaagag cggcatcgcg cagcgtcacg gctgaagcag
     2161 cggcccggtg cgcagggccg gcgcaggcca tggaaacagc aagtcacggt tttgatacgg
     2221 aagcgtgaaa cttccggatg aacggaagtt cagaatccgc attgattcca cgtccgccaa
     2281 ccctgcacag cgaggaacgc accagcgccc gggtacgggt ccaggccagg tggggcagac
     2341 acgaacagat gcggaggcca gcatgtccgg cccgaatcga aagctcgacc gaaacgaccg
     2401 ttatttccgc ctgccgtccg ccggaagccc ggtcaggctg ccggcggcgg tggcgcatgg
     2461 cgcgcagccg cggctggagg actggtgcct gatcgccggg gatcgcctga gcggccgggt
     2521 ctacgacgat ccgcgtttcg aggacggcga gcacatcacc accagtccgg tgctgctgct
     2581 cagcaatcac gccgcggcga cgtgcacgac gcactatcgc ctgggccggc ccgcctgaat
     2641 ccattccttc gcaagcacac cggtcccagc agacagccgg cgacgttccg gtcgccggat
     2701 ctcagagccg tgacagcagt tctgccttct tggcgttgaa ttcatcgtcg ctgatgaaac
     2761 ccttcgcgcg caggccggcg agtttctcga tggtcgcgaa gatgtcggat tcgctggcgg
     2821 tgccggatgc agcagccggt gccggcgagt tcatcggcgc aggggaggac ggtgacgatg
     2881 acggggacca ggcatccggc tgcctgccgc cgccggaaat caccggcagg ctggagaggt
     2941 tcacggtgcc gtactggctc gtgaagctca gcgtgccgga gctgccctgc tgttgcgaga
     3001 cgccgccgat ctggtgatca agcgtgtcgt agatcgtcac gtggccaccg gtttcgatgg
     3061 cgaggcggtg ggcctgcgcg aagtacgcat agcgcacgcc gttctgcgcc ccggtgctgt
     3121 tcggccagcg cagctccgga ccccaccagt ccgcctgcgg cgtgaacagg ctggagccag
     3181 tgccggactg accggacggc gggttccagt tgccctgctg ctggtacgaa tcgttgtggc
     3241 cgctgccctg gctctgcgac tggaagctgc cggtctgcag caggccgggc tggttcgcga
     3301 ccagcgtcga gagttcctgg cagagggcat cgacgcgcga cttcaggccg tggttgaaca
     3361 tgtcgcccag catcagcatg ccgccggcca tccactggcc cgaaccaccg aattcgtggt
     3421 ggctgaactg cgccatcgtg ccgttgccgt tgatgacggc gtcgagcatg gcgcgtacgg
     3481 catcgctgct gaagccatga cggcgggaaa tatcgtcgac ggcctgctgg ccggcggggg
     3541 aaagctgtcg catcggattc atctcgcttc cggacgggca ggggttgttc gccacggtcc
     3601 ggtcagatag ccgtatttac ggcggggggc gagtctagga gg
//
LOCUS       DAOQJH010000228         3641 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_16035_length_3641_cov_4.190742, whole genome shotgun sequence.
ACCESSION   DAOQJH010000228 DAOQJH010000000
VERSION     DAOQJH010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3641)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3641)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3641
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16035_length_3641_cov_4.190742"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..433)
                     /locus_tag="PLQ18_06335"
     CDS             complement(<1..433)
                     /locus_tag="PLQ18_06335"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNE01307.1"
                     /translation="MSSSPAPAAVLAEPPLRSMAAFRHFWLTRVCTTIAYQMIGVAVG
                     WRVYELTGSALALGLIGLAQFLPALLLVLPAGQVADRHDRRRVAARAQRVSALAAALL
                     ALGSLGDWLTVHGIYALVLVIGAARAFEAPSLQALLPRLVPP"
     gene            complement(671..2083)
                     /locus_tag="PLQ18_06340"
     CDS             complement(671..2083)
                     /locus_tag="PLQ18_06340"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNE01308.1"
                     /translation="MPVTIGKYRIESVLGHGAMGVVYRGHDPVLDRTVAIKTIRAELL
                     AAGDDGFEDDDWHARFRHEARAAARCTHPNIVTIYDFGETPDGSPYLVMEFVEGRELR
                     RHLGEGRQFDLPAAALILRQILRALDCAHLLGIVHRDIKPANVILLADGMVKVTDFGI
                     ARLSGGTGLTQTGAMVGTPNYMSPEQFTGGTLDRRADLYACAVIAYELLTGQRPYPGP
                     TPPALLHQILNTTPPRPSALRPSLPAALDAVLLRGLARDPGARFADACEFQRALDAAL
                     APALAPVPIHHHPEPGTVVMPAPLTLSDAELHEAETTLATHIGPLARVLVRRAAASAH
                     SRDAWLEEIARNISDDDERRRFLRRLRRGEPTGYTQYGTTAHTAHTGHVGHAGGNAAP
                     TAASGYGSSGSVTTVLRVDAATLATAQQALTQAIGPIARVLVRQTAPLAQDAADLYRR
                     LAERIDDADARRRFLATRPH"
     gene            complement(2249..3121)
                     /locus_tag="PLQ18_06345"
     CDS             complement(2249..3121)
                     /locus_tag="PLQ18_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007871282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HNE01309.1"
                     /translation="MGLRTKFNLVLILSFGIGIGLSGYLAYGLLRENAREEVLQNARI
                     MVEGALAIRGYTSREIQPLLTLPMKRQFLPQTVSSYAAQTNFRTLREHFPEYTYKEAA
                     LNPTNLNDRAADWEADIIREFRNNAERKEIITERETPTGRVLTLARPLKVGSPACLTC
                     HSTPDAAPDTMKAVYGTANGFGWQLNEIVGAQIVSIPMAVPLDRAEKVFHTFLAGLGA
                     IFLLLLVLLNLLLHFAVIRRVRRIATMADKVSLGEEDVPELDARGSDEIARLAASFTR
                     MRRSLENAMRLLGE"
     gene            complement(3139..>3641)
                     /locus_tag="PLQ18_06350"
     CDS             complement(3139..>3641)
                     /locus_tag="PLQ18_06350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE01310.1"
                     /translation="TTLPVTAWELRPAGKDPLSGQTRCLLVEEPLTINDGYGDTRIDI
                     LFNGEALIVVTDSEIDAGFNDLALSVAIERRPPARGQKIVEPPPSPRFVPARIENKKT
                     LVFTDAPAIEEAFKAGSTASFALRFWPLWPSVGSIPVEVSLKGFTKVAEEFSRCTGQP
                     AAGTTR"
BASE COUNT          515 a         1181 c         1409 g          536 t
ORIGIN      
        1 cgggcggcac cagccgcggc agcagcgcct gcagcgacgg cgcctcgaag gcgcgcgccg
       61 cgccgatcac gagcaccagc gcatagatgc cgtgcaccgt cagccagtcg ccgaggctgc
      121 cgagcgcgag cagcgccgcg gcgagcgccg acacgcgctg cgcccgggcc gcgacccgcc
      181 gccggtcgtg gcggtcggcg acctgaccgg ccggcagcac cagcagcagc gccggcagga
      241 actgcgcgag cccgatcagc ccgagcgcca gcgcgctgcc ggtcagctcg tagacgcgcc
      301 agccgaccgc gacgccgatc atctgatagg caatcgtggt gcagacgcgc gtcagccaga
      361 agtgtcggaa cgccgccatc gagcgcagcg gcggctcggc gaggactgcg gcaggagcgg
      421 gggaggagga catcgcagca gtacagtccg tgggcagggg cgggttgccg ggcagtgtac
      481 tgcgctgcaa cagcgcgccc gtgcgccgat cgctgcatgc cggacatgca ggcgctgcgg
      541 ccctgcgacg gcgggtgaac cgggggagga agcggaagaa agcgggaggg acgcggcaga
      601 tggggtgcga aggcacgcgg gacggatgcg ccgcggacat gcgcgtcgcc cgcccgcccg
      661 ggcgccggtc tcagtgcggg cgggtggcga ggaagcgccg gcgcgcgtcg gcgtcgtcga
      721 tgcgctcggc gaggcgccgg tacaggtcgg cggcgtcctg cgccagcggt gccgtctggc
      781 gcaccagcac gcgcgcgatc ggcccgatcg cctgcgtcag cgcctgctgc gcggtggcga
      841 gtgtggccgc gtcgacgcgc agcaccgtcg tcaccgagcc gctgctgccg tagccggaag
      901 cggcggtcgg tgcggcgttg ccgccggcgt ggccgacgtg accggtgtgg gcggtatgcg
      961 cggtggtgcc gtactgcgtg tagccggtcg gttcgccgcg gcgcagccgg cgcaggaagc
     1021 gccggcgctc gtcatcgtcg ctgatgttgc gggcgatctc ttcgagccag gcatcgcgcg
     1081 agtgcgccga ggcggcggcg cggcgcacca gcacgcgcgc gagcggaccg atgtgcgtcg
     1141 ccagcgtggt ttcggcctcg tgcagctcgg cgtcgctgag cgtcagcggt gccggcatca
     1201 cgacggtgcc gggctccgga tggtggtgga tcggcaccgg cgcgagggcc ggggcgagcg
     1261 cggcatcgag cgcgcgctgg aattcgcagg catcggcaaa gcgtgcgccc gggtcgcggg
     1321 ccaggccgcg cagcagcacg gcatcgagcg ccgccggcag gctgggacgc agcgcgctcg
     1381 ggcgcggcgg cgtcgtgttc aggatctggt gcagcagcgc cggcggcgtc ggacccggat
     1441 acggccgctg gccggtcagc agctcatagg cgatcaccgc gcaggcgtag agatcggcgc
     1501 gccggtcgag cgtgccgccg gtgaactgct cgggcgacat gtagttcggc gtgccgacca
     1561 tcgcgccggt ctgggtcagg ccggtgccgc cgctcaggcg cgcgatgccg aagtcggtga
     1621 ccttgaccat gccgtcggcg agcaggatga cgttggccgg cttgatgtcg cgatgcacga
     1681 tgccgagcag gtgcgcgcag tcgagcgcgc gcaggatctg gcgcaggatc agcgccgccg
     1741 ccggcaggtc gaactggcgg ccctcgccga ggtggcggcg cagctcgcgg ccctcgacga
     1801 actccatgac cagatacggt gaaccgtccg gcgtctcgcc gaagtcgtag atcgtgacga
     1861 tgttcgggtg cgtgcagcgc gcggcggcgc gcgcctcgtg gcggaagcgc gcgtgccagt
     1921 catcgtcctc gaagccgtcg tcgccggcgg cgagcagttc ggcacggatg gtcttgatcg
     1981 cgaccgtgcg atcgagcacc ggatcgtggc cgcggtagac gacccccatc gcaccatgcc
     2041 cgagcaccga ctcgatgcgg tacttgccga tggtgacggg catcgtgctc acggtcgtgc
     2101 ctccgacgag cggacgccgg cgcgtgcgac ggggcggcgg ggcaggcagg tccgccgccg
     2161 gcagcggggt gaggcaggtt ccgtcataag cttcgtatcc ggcggcggct gacatcggca
     2221 gtatccctct ttcgaggcgg gattcgcgtc attcgcccag cagccgcatc gcgttttcga
     2281 ggctgcggcg catgcgcgtg aacgaggccg cgagcctggc gatttcatcg ctgccgcggg
     2341 catcgagttc cggcacgtcc tcctcgccga ggctgacctt gtcggccatg gtcgcgatgc
     2401 ggcgtacgcg gcggatgacg gcgaagtgca gcagcaggtt cagcagcacc agcagcagca
     2461 ggaagatcgc accgaggccg gcgaggaagg tgtgaaagac tttctcggcg cgatcgagcg
     2521 gcaccgccat cgggatcgag acgatctgcg cgccgacgat ctcgttgagc tgccagccga
     2581 agccgttggc ggtgccgtag acggccttca tcgtgtcggg cgcagcgtcg ggcgtgctgt
     2641 ggcaggtcag gcaggcgggg ctgccgacct tcagcggccg ggcgagcgtc agcacgcggc
     2701 cggtcggtgt ttcgcgttcg gtgatgatct ccttgcgttc ggcgttgttg cggaactcgc
     2761 ggatgatgtc ggcttcccag tcggccgcgc ggtcgttgag gttggtcggg ttcagcgctg
     2821 cttccttgta cgtgtactcg gggaagtgct cgcgcagcgt gcggaagttg gtctgcgcgg
     2881 cgtacgagga cacggtctgc ggcaggaact ggcgcttcat cggcagcgtc agcagcggct
     2941 ggatctcgcg cgaggtgtag ccgcggatcg cgagcgcgcc ctcgaccatg atccgcgcgt
     3001 tctgcaggac ttcctcgcgg gcgttttcgc gcagcagccc gtaggcgagg tagccggaaa
     3061 ggccgatgcc gatgccgaag ctcaggatca gcaccaggtt gaatttggta cgcaggccca
     3121 tcggtgttcg tccccctgtc agcgggtggt gccggcggcg ggctggccgg tgcagcggga
     3181 aaattcctcg gccaccttcg tgaagccctt gagactgacc tcgaccggga tcgagccgac
     3241 cgagggccac agcggccaga agcgcagcgc gaagctcgcg gtgctgccgg ccttgaaggc
     3301 ttcctcgatc gccggtgcgt ccgtgaacac cagcgtcttc ttgttctcga tacgcgcggg
     3361 aacgaagcgt ggagacggtg ggggctcgac gattttctgt ccgcgcgccg gcggccggcg
     3421 ctcgatcgcg acgctcagcg ccaggtcgtt gaagccggcg tcgatctcgg aatcggtgac
     3481 gactattaat gcctcgccgt tgaacaggat gtcgatacgc gtgtcgccgt agccgtcgtt
     3541 gatggtgagc ggttcctcga ccagcaggca gcgcgtctgg ccgctcagcg ggtccttgcc
     3601 ggccgggcgc agctcccagg ccgtcaccgg cagggtcgtc a
//
LOCUS       DAOQJH010000229         3635 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_16074_length_3635_cov_4.215084, whole genome shotgun sequence.
ACCESSION   DAOQJH010000229 DAOQJH010000000
VERSION     DAOQJH010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3635)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3635)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3635
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16074_length_3635_cov_4.215084"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            306..1094
                     /locus_tag="PLQ18_06355"
     CDS             306..1094
                     /locus_tag="PLQ18_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014087259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HNE01311.1"
                     /translation="MGSYENLLLESPDPGIHVLTLNRPRALNALNRATIAELHAAVQA
                     IAADADARVLLVTGAGGRAFVAGADIKEMQGYTAIEAQAFARRTMAVLRAIETLPVPV
                     IAVVDGYALGGGNELAMSCDFILASERAVFGQPEVSLGVTAGFGGTQRLTRLVGRARA
                     LELLLSGRHVDAAEALRIGLVNRVCPAATLMDEALALARSIAAQGPVAVRLSKEAVQR
                     GQDLDLDNACQFEAQLFGLCFATADQKEGMQAFIDKRTAQFLNR"
     gene            1110..2114
                     /locus_tag="PLQ18_06360"
     CDS             1110..2114
                     /locus_tag="PLQ18_06360"
                     /EC_number="1.3.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017916519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acryloyl-CoA reductase"
                     /protein_id="HNE01312.1"
                     /translation="MVSDAAFAALRAHRVDGRVESRLETLVIADLNPGDVSVCVAWSS
                     LNYKDALACTGAGRIQKRFPLVGGIDLAGHIAASRDPRFREGDAVLVIGCGLGEEADG
                     GYAGYANVCGDWLVPLPAGLTLREAMAIGTAGFTAALGIVRLEDNGQSPAHGPMLVSG
                     ATGGVGSFAIDLLAGLGYEVAALTGKPDEHAYLQQLGATQIVDRSTLDPGSRPLETAR
                     WGGAIDNLGGTTLAWLTRTIRPFGNIACIGLAQDHRLETSVMPLILRGISLIGINSTY
                     CPPAWRARTWARLAADLKPQHLACIVTGELTLEALPAACAALLGGRATGRSVVRIGGG
                     "
     gene            2252..3577
                     /locus_tag="PLQ18_06365"
     CDS             2252..3577
                     /locus_tag="PLQ18_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNE01313.1"
                     /translation="MKFAARLRLAIICALSITAPLPALADGEQFIPITSFRVGPNAGV
                     GSAVFGGFIDYLNLINARDGGVNGVKLTYAECETEYKVERTVECYEELKKRGPSGASM
                     FNFVATGPVYALLERAAADHIPLVSIGYGRSDSADGRVFPYLFPLMTTYWSQSTAKIR
                     FIGQREGGMDKLRGKKIVNLYHESAYGKETLPILESQAQKYGFTLVNIPVPPPGASQQ
                     AEWTRIKREKPDWVILRGFGVMNPTALIFAQRFGFPADHIVGVWWAGAEEDVVPAGQT
                     AKGYIAASLNPPGDNFPAIQQIRSLLYAKGQGNMSDPARIGSVYYNRGIVHGVLNVEA
                     IRVAQGRFGKKPLTGEQVRWGLEHLNLTPERLKELGVEGLMPSLKTSCSDHEGHAPVR
                     FMQWDGRTWNLISDFIETDSSLVLPLVAQSAADYAREKGVTPRDCSKDD"
BASE COUNT          571 a         1229 c         1263 g          572 t
ORIGIN      
        1 cgtcgaggtc aagcggctgt aggtcgtcca ggttccacga ttcacctgca gcaaggcccc
       61 ggcgcttacc ggggcttttt catttcgcct caggcgcatc catgccggat tgctcgcggg
      121 tctccggtcc gcgcaccgcc aggagcggtt gttcctgcgt gcgaagacgc cgtgatccgc
      181 aaacggaagc cgcagcgccg gaccttgatc cggacaggca ccgggagcga gcggatgcct
      241 tgccagtgcg cctccggctc cagcgtctat gaatgtggac tcctgccgtc accggagtcc
      301 ccgccatggg cagctacgaa aacctcctgc tcgaatcgcc cgatccgggc atccacgtgc
      361 tgacgctgaa ccggccacgg gcgctgaatg cgctgaaccg agcgaccatc gccgagctgc
      421 atgccgccgt gcaggccatc gctgccgatg ccgatgcgcg tgtgctgctc gtcaccggtg
      481 ccgggggccg ggccttcgtc gccggcgccg acatcaagga gatgcagggt tacacggcga
      541 tcgaggcgca ggcgttcgcg cggcgcacga tggcggtgct gcgtgccatc gagacgctgc
      601 cggtgccggt gattgcggtc gtcgatggct atgcgctcgg tggcggcaac gagctggcga
      661 tgagctgcga cttcatcctc gcctcggagc gcgcggtgtt cggccagccc gaggtctcgc
      721 tcggcgtcac ggccggcttc ggcggcacgc agcggctgac gcggctggtt ggccgggcgc
      781 gggcgctgga gctgctgctg agcggccggc acgtcgatgc cgccgaggcg ctgcgcatcg
      841 ggctggtcaa ccgggtctgc ccggccgcga cgctgatgga cgaggccctc gcgctggcgc
      901 gcagcatcgc cgcgcagggg ccggtcgcgg tgcgtctgag caaggaggcc gtgcagcgcg
      961 gacaggacct cgatctcgac aacgcctgcc agttcgaggc gcagctgttc ggtctctgct
     1021 tcgcgaccgc cgaccagaag gaaggcatgc aggccttcat cgacaagcgc accgcgcagt
     1081 tcctgaaccg ctgaggggct cggattgcca tggtctccga tgccgcgttt gcggccctgc
     1141 gtgcccaccg cgtcgacggc cgcgtcgaat cccggctcga aacgctcgtc atcgccgacc
     1201 tgaaccccgg cgacgtgagt gtgtgcgtcg cgtggtcgag cctgaactac aaggacgcgc
     1261 tggcctgcac cggggccggg cgcatccaga agcgctttcc gctggtcggc ggcatcgatc
     1321 tcgccggtca catcgcggcc tcgcgtgatc cgcgctttcg cgaaggcgat gcggtgctcg
     1381 tgatcggctg cgggctgggc gaggaggccg atggtggtta tgccggttat gcgaatgtct
     1441 gcggtgactg gctggtgccg ctgccggcgg gcctgacgct gcgcgaggcg atggcgatcg
     1501 gcacggccgg cttcaccgcc gcgctcggca tcgtgcgcct cgaagacaac ggccagtcgc
     1561 cggcgcacgg gccgatgctg gtcagcggcg cgaccggcgg ggtcggcagc ttcgcgatcg
     1621 acctgctcgc cgggctcggt tacgaagtgg cggcgctgac cggcaagccg gatgagcacg
     1681 cctacctgca gcaactcggt gccacgcaga tcgtcgatcg cagcacgctc gatcccggca
     1741 gccggccgct ggagaccgca cgctggggcg gggcgatcga caacctcggc ggtacgacgc
     1801 tggcgtggct gacccggacg atccggccgt tcggcaacat cgcctgcatc ggtctggcgc
     1861 aggatcaccg gctggagact tccgtgatgc cgctgatcct gcgcggcatc agcctgatcg
     1921 gcatcaactc cacgtattgc ccgccggcct ggcgggcgcg gacctgggcg cggctggcgg
     1981 cggacctgaa accgcagcat ctggcctgca tcgtcaccgg tgaactgacg ctggaggcat
     2041 tgccggcggc ctgcgcggcg ctgctcggcg ggcgcgcgac cgggcgcagc gtcgtgcgca
     2101 tcggcggcgg ctgagccgct cgcaggcatg ccggcacgaa cctgaaactt cgttcatttc
     2161 agacgatgct gcgcattgca cactgcggta acctcgccgc agccggctgg agccagccgg
     2221 ctgctttcag gacgacgggg aggggaccga catgaagttc gccgcaagac tgcgccttgc
     2281 gatcatctgt gcgctgagca tcaccgcccc gctgccggcc ctggccgatg gcgagcagtt
     2341 catcccgatc acgagctttc gcgtcggccc gaacgccggc gtcggcagcg ccgtgttcgg
     2401 tggtttcatc gattacctga acctgatcaa tgcccgcgac ggcggcgtca acggcgtgaa
     2461 gctcacgtat gccgagtgcg agaccgagta caaggtcgag cgcaccgtcg agtgctacga
     2521 ggaactgaag aaacgcggcc cgagcggcgc gtcgatgttc aacttcgtcg ccaccgggcc
     2581 ggtgtacgcg ctgcttgagc gcgccgcggc cgatcacatt ccgctggtgt cgatcggcta
     2641 cggccgctcg gattcggccg acgggcgcgt gtttccgtac ctgttcccgc tgatgaccac
     2701 gtactggtcg cagagcacgg ccaagatccg cttcatcggc cagcgcgaag gcggcatgga
     2761 caagctgcgc ggcaagaaga tcgtcaacct ctaccacgag tcggcctacg gcaaggaaac
     2821 cctgccgatc ctcgaatccc aggcgcagaa gtacggcttc acgctggtca acatcccggt
     2881 gccgccgccc ggcgccagcc agcaggcgga atggacgcgg atcaagcgcg agaagcccga
     2941 ctgggtgatc ctgcgcggct tcggcgtcat gaacccgacc gcgctgatct tcgcgcagcg
     3001 cttcggcttc ccggccgatc acatcgtcgg cgtctggtgg gccggtgccg aggaggatgt
     3061 cgtcccggcc gggcagaccg ccaaaggcta cattgccgcg agcctgaacc cgcccggcga
     3121 caactttccg gccatccagc agatccgctc gctgctctat gcgaagggcc agggcaacat
     3181 gagcgatccg gcacgcatcg gcagcgtcta ctacaaccgc ggcatcgtgc acggcgtgct
     3241 gaacgtcgag gcgatccgcg tcgcgcaggg caggttcggc aagaagccgc tgaccggcga
     3301 acaggtgcgc tggggcctcg aacacctgaa cctgacgccg gaacggctga aggagcttgg
     3361 cgtcgagggg ctgatgccct cgctgaagac ctcgtgcagc gaccacgaag gccatgcgcc
     3421 cgtgcgcttc atgcaatggg atggccgcac ctggaacctg atctcggact tcatcgagac
     3481 cgactcgtcg ctggtgctgc cgctggtggc gcagtcggcg gccgactacg ccagggaaaa
     3541 aggcgtcacg ccgcgcgact gcagcaagga cgactgaaaa gccccgtcgg ccggcacccg
     3601 ccggccttgt cccctgcgca gggccaccat gcaag
//
LOCUS       DAOQJH010000230         3627 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_16116_length_3627_cov_5.233763, whole genome shotgun sequence.
ACCESSION   DAOQJH010000230 DAOQJH010000000
VERSION     DAOQJH010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3627
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16116_length_3627_cov_5.233763"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..567
                     /locus_tag="PLQ18_06370"
     CDS             <1..567
                     /locus_tag="PLQ18_06370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Catalyzes the transfer of electrons from NADH to
                     ubiquinone; Derived by automated computational analysis
                     using gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit G"
                     /protein_id="HNE01314.1"
                     /translation="PVAACVETSGTFVNLEGRWQDFQAVARVPGQVRPGWKVLRVLGN
                     LLGVAGFDHLSSADVAAEARSAADGIAATGAVEVAPADVQPAGEGLACIVEVPIYAID
                     PIVRRAAALQASPLAVSAEVRIGTAGAGQFGLTADARASVGRDGNAVVLPVKIDDRIP
                     DGCVWVPAGVEATGALGAGAGPVEVRPA"
     gene            676..1698
                     /gene="nuoH"
                     /locus_tag="PLQ18_06375"
     CDS             676..1698
                     /gene="nuoH"
                     /locus_tag="PLQ18_06375"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006748483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HNE01315.1"
                     /translation="MDSLLTLVLILLKIVAIVAPLMLSVAYLTYAERKIIGYIQVRIG
                     PNRVGPKGWLQPIADAVKLMMKEIVIPTGANRFLFVLAPVLSIGPALAAWAAIPLGDG
                     IVLSHIDAGLLYLLAMTSLGVYGVIIAGWASNSKYSFLGAMRSAAQIVSYEIAMGFAL
                     VGVLMAGGSLNLNEIVRAQSGSVLHWFWLPLLPLFIVYFISGVAETNRAPFDVAEGES
                     EIVAGFHVEYSGMTFAVFFLAEYANMILISALAATMFLGGWLSPFQGIPLLGALFTWV
                     PGAVWLILKTAVFLFLFLWLRATFPRYRYDQIMRLGWKVFIPVTIVWLAVVGVAKWAH
                     LAPWFD"
     gene            1720..2208
                     /gene="nuoI"
                     /locus_tag="PLQ18_06380"
     CDS             1720..2208
                     /gene="nuoI"
                     /locus_tag="PLQ18_06380"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013219634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HNE01316.1"
                     /translation="MAALKHYFRSFLLWELFIGLRLTGKYLFARKITVQYPEEKTPMS
                     PRFRGLHALRRYPNGEERCIACKLCEAVCPALAITIDMQQREDGTRRTTRYDIDLFKC
                     IFCGFCEESCPVDSIVETRFFEYAFEKREESIMTKPKLLAHGDRMEASIAADRAADAR
                     YR"
     gene            2230..2832
                     /locus_tag="PLQ18_06385"
     CDS             2230..2832
                     /locus_tag="PLQ18_06385"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HNE01317.1"
                     /translation="MGFEKFLFYVFAIVLVFASVRVITVRNPVHAALFLVLAFFTSAA
                     LWLLLQAEFLAIALVLVYVGAVMVLFLFVVMMLDINVAPLREGFIRNAPLGIAVATII
                     VIEMALVLTSDRSGLVSGALQNYAADHSNTKELGSVMYTLYAYPFEIAALILLVAIVA
                     AIGLTMRRRQGVRYVDPAEQVAVRREGRVRLVNLAAEKKS"
     gene            2924..3229
                     /gene="nuoK"
                     /locus_tag="PLQ18_06390"
     CDS             2924..3229
                     /gene="nuoK"
                     /locus_tag="PLQ18_06390"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HNE01318.1"
                     /translation="MIALTDYLVLGAILFCLSVAGIFLNRKNVIILLMSIELMLLAVN
                     FNFIAFSRFLGDSAGQVFVFFILTVAAAESAIGLAILVVLFRNRRTINVGDLDTMKG"
     gene            3234..>3627
                     /locus_tag="PLQ18_06395"
     CDS             3234..>3627
                     /locus_tag="PLQ18_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019584281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HNE01319.1"
                     /translation="MQTVYLSLVLAPLFGAIIAGLFGRQIGRSAAHWVTIIGVAISFL
                     LSLAVFKHHVIDGAPAYNATVYTWMVIDGIRLEVGFLVDSLTVMMITVVTFVSLMVHI
                     YTIGYMHDDPGYQRFFSYISLFTFSMLML"
BASE COUNT          533 a         1193 c         1191 g          710 t
ORIGIN      
        1 ccggtcgcgg cctgcgtcga aacctccggc accttcgtca acctcgaagg ccgctggcag
       61 gacttccagg ccgtggcgcg cgtgcccggt caggtccggc ccggctggaa ggtgctgcgc
      121 gtgctcggca acctgctcgg cgtcgccggt ttcgaccatc tcagctcggc cgacgtggct
      181 gccgaggcgc gcagcgccgc ggacggcatc gccgcgaccg gagcggtcga ggtcgcgccc
      241 gccgatgtgc agccggccgg ggaggggctg gcgtgcatcg tcgaggtgcc gatctacgcg
      301 atcgatccga tcgtgcgccg tgccgccgcg ctgcaggcct cgccgctcgc cgtgtccgcc
      361 gaagtccgca tcggcaccgc cggtgccggg cagttcgggc tgaccgccga tgcgcgtgcc
      421 agcgtcgggc gcgacggcaa tgccgtggtt ctgcccgtga agatcgacga ccgcattccg
      481 gacggctgcg tctgggtgcc ggccggcgtg gaagcgaccg gagcgctcgg tgcgggcgcc
      541 ggtccggtcg aggtccgccc ggcctgagcg ccgacacccg acaccgaggg ggggaacgac
      601 ggggggcctg ccgcacggca ggcaccccgg cgttgcgatc cggaacagaa tgacaagctc
      661 gacaggacag agaccgtgga cagccttttg accctggtgc tgatactcct caagatcgtc
      721 gcgatcgtcg cgccgctgat gctgtcggtc gcgtatctga cctatgccga acgcaagatc
      781 atcggctaca tccaggtgcg catcggcccg aaccgcgtcg gccccaaggg ctggctgcag
      841 ccgatcgccg acgccgtgaa gctgatgatg aaggaaatcg tcatcccgac cggcgcgaac
      901 cgcttcctgt tcgtgctggc gccggtgctg tcgatcggcc cggcgctcgc ggcctgggcg
      961 gcgatcccgc tcggcgacgg catcgtgctg tcgcacatcg acgccggcct gctgtacctg
     1021 ctggcgatga cctcgctcgg cgtgtacggc gtcatcatcg ccggctgggc ctcgaactcg
     1081 aagtattcct tcctcggcgc gatgcgctcg gccgcgcaga tcgtctcgta tgaaatcgcg
     1141 atgggcttcg cgctggtcgg cgtgctgatg gccggtggca gcctgaacct caacgagatc
     1201 gtccgcgcgc agtcgggcag cgtgctgcac tggttctggc tgccgctgct gccgctgttc
     1261 atcgtgtatt tcatctccgg cgtcgccgag accaaccgcg cgccgttcga cgtggccgag
     1321 ggcgaatccg aaatcgtcgc cggcttccac gtcgagtact cgggcatgac cttcgcggtg
     1381 ttcttcctcg ccgaatacgc gaacatgatc ctgatctcgg cgctcgccgc gacgatgttc
     1441 ctcggcggct ggctgtcgcc gttccagggc atcccgctgc tcggcgcgct gttcacgtgg
     1501 gtgcccgggg cggtgtggct gatcctgaag acggccgtgt tcctgttcct gttcctgtgg
     1561 ctgcgtgcga ccttcccgcg ctatcgctac gaccagatca tgcgtctcgg ctggaaggtg
     1621 ttcattccgg tgacgatcgt gtggctggcc gtggtcggcg tggcgaagtg ggcgcatctg
     1681 gcgccgtggt tcgactgagg ccggaggggc gacaaagcaa tggctgctct caaacactat
     1741 ttccgcagtt tcctgctctg ggagctgttc atcggtctgc gcctgaccgg caagtacctg
     1801 ttcgcgcgca agatcacggt gcagtacccg gaagagaaga cgccgatgag cccgcgcttc
     1861 cgcggcctgc acgcgctgcg ccgctatccg aacggcgagg agcgctgcat cgcctgcaag
     1921 ctctgcgagg cggtctgccc ggcgctggcg atcacgatcg acatgcagca gcgcgaggac
     1981 ggcacgcgcc ggaccacgcg ctacgacatc gacctgttca agtgcatctt ctgcggattc
     2041 tgcgaggagt cctgtccggt cgattcgatc gtcgagacgc gcttcttcga gtacgccttc
     2101 gagaagcgtg aggaatcgat catgaccaag ccgaagctgc tcgcgcacgg cgaccgcatg
     2161 gaagcctcga tcgctgccga ccgcgccgcc gacgcgcgct accgctgagc cgccacggga
     2221 accgagatca tgggcttcga gaaattcctg ttctacgtct tcgccatcgt gctggtgttc
     2281 gcgtcggtgc gggtcatcac cgtgcgcaac cccgtgcatg cggcgctgtt cctggtgctg
     2341 gcgttcttca ccagcgcggc gctctggctg ctgctgcagg ccgagttcct ggccatcgca
     2401 ctggtgctcg tgtacgtcgg cgccgtcatg gtgctgttcc tgttcgtcgt gatgatgctc
     2461 gacatcaacg tcgcgccgct gcgggagggc ttcatccgca acgcaccgct cggcatcgcc
     2521 gtcgcgacga tcatcgtcat cgagatggcg ctggtgctga cctcggatcg ctccgggctg
     2581 gtcagcggtg cgctgcagaa ctacgcggcc gatcacagca acaccaagga actcggcagc
     2641 gtcatgtaca cgctctacgc gtacccgttc gagattgccg cgctcatcct gctggtcgcc
     2701 atcgtcgccg cgatcggcct gacgatgcgt cgccgtcagg gcgtgcgcta cgtcgatccg
     2761 gcagagcagg tggccgtgcg gcgcgagggc cgggtgcgac tcgtcaatct ggcagccgaa
     2821 aagaagagct gagagcgggt cgtgcgcacc gccggttgca gccgcagatc catgcggctg
     2881 caacggtgtc cggaattaca gaaacattgc gttaggggga tggatgatcg cgcttactga
     2941 ttacctggtg ctcggggcga tcctcttttg tctcagcgtg gcgggcattt tcctgaaccg
     3001 gaaaaatgtc atcatcctgc tgatgtccat cgagttgatg ctgctggcgg tcaatttcaa
     3061 cttcatcgcg ttttcccgct ttctcggcga cagcgccggg caggtgttcg tgttcttcat
     3121 tcttaccgtg gcggccgcgg aatcggcgat cggcctcgcg atcctggtgg tgctgttccg
     3181 caaccgccgc acgatcaacg tcggcgatct cgacacgatg aagggttgag cgcatgcaga
     3241 ccgtctatct ctccctcgta ctcgcgccgc tgttcggtgc catcatcgcc ggcctgttcg
     3301 gccggcagat cggccgcagc gccgcgcact gggtcacgat catcggtgtc gcgatctcgt
     3361 tcctgctgtc gctcgcggtg ttcaagcacc acgtcatcga cggcgcgccg gcctacaacg
     3421 ccaccgtcta cacgtggatg gtcatcgacg gcatccggct cgaagtcgga ttcctcgtcg
     3481 attcgctgac cgtcatgatg atcaccgtcg tgaccttcgt gtcgctgatg gtgcacatct
     3541 acacgatcgg ctacatgcac gacgatccgg gctaccagcg cttcttcagc tacatctcgc
     3601 tgttcacgtt ctcgatgctg atgctgg
//
LOCUS       DAOQJH010000231         3620 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_16165_length_3620_cov_5.102384, whole genome shotgun sequence.
ACCESSION   DAOQJH010000231 DAOQJH010000000
VERSION     DAOQJH010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3620)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3620)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3620
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16165_length_3620_cov_5.102384"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..528
                     /locus_tag="PLQ18_06400"
     CDS             <1..528
                     /locus_tag="PLQ18_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014337456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimV/HubP family polar landmark protein"
                     /protein_id="HNE01320.1"
                     /translation="PVPAPATTPEDLLADLGWSDADLGSPPAMPEKPVTAAPAPRPAE
                     VADLDFGDDLAAFLPPRQTSTPAQAAAKPAAVDPATAELADDLGFSFQPVSAPAPAPL
                     PVVPAPAAAPPVSASTADTRGEDEFVETKLDLALAYIDMDDPLGAKSLLEEVLQEGGS
                     SQRSRAQALLERLAS"
     gene            complement(611..1435)
                     /gene="folE2"
                     /locus_tag="PLQ18_06405"
     CDS             complement(611..1435)
                     /gene="folE2"
                     /locus_tag="PLQ18_06405"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002808786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0035998 - 7,8-dihydroneopterin
                     3'-triphosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HNE01321.1"
                     /translation="MPTAVPAPQQQQPAAIPDIQSTPDTRRLPIDKVGVKDLRHPVRV
                     RDRSDGEQHTVATFNMYVRLPHDFKGTHMSRFVEVLNRPGREIGVASFHEMLTEMNAR
                     LDAGTGHIEMSFPYFVMKTAPVSGVQSLLDYQVTLIGELAGEQATTWLRVVVPVTSLC
                     PCSKEISRYGAHNQRSHVTVSARLSGFVWIEELIDIVEQEASCELYGLLKRPDEKYVT
                     ERAYENPKFVEDMVRDVAARLNDDDRIAAYVIESENFESIHNHSAYALIERDKTAG"
     gene            complement(1622..3490)
                     /gene="dxs"
                     /locus_tag="PLQ18_06410"
     CDS             complement(1622..3490)
                     /gene="dxs"
                     /locus_tag="PLQ18_06410"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010960141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HNE01322.1"
                     /translation="MTALTPVFPLLDTIDSPQDLRRLPENVLGQLANELREFLIQSVA
                     RTGGHFSANLGTVELTIALHYVYDTPEDRLVWDVGHQSYPHKILTGRRALMGTMRRRH
                     GLAGFPKRCESPYDTFGVGHSSTSISAAQGMAIAAEAAGSRRRAVAVIGDGAMTAGMV
                     FEALNHAGHLKNDILVVLNDNEMSISPNVGALSSYLTRLLSGHVYSTVREGGKRILAH
                     LPSSMWEMARRAEEHAKGLVTPGTLFEELGFNYFGPIDGHDLPTLVRTLENLRDQKGP
                     RLLHVMTRKGKGYKLAENEPVTYHGVGSFDPASGIPPAKTGGGPTYTQVFGEWLCDMA
                     AQDPRLVGITPAMREGSGLVEFAERYPTRYFDVGIAEQHAVTLAAGMACDGLKPVVAI
                     YSTFLQRAYDQLIHDVAIQNLPVLFAIDRAGLVGPDGPTHAGSFDLCYLRCVPNLLIM
                     APADEDECRQMLYTGFLHDGPACVRYPRGKGPGVAPVATMTALPIGRAELRRQGRGTA
                     LLAFGAMLPLAEAVGERLDATVVNMRFVKPLDTAMVLELARTHERLVTIEDGAVMGGA
                     GSAVAECLAGHGVHTALKLLGLPDRFLDHGERGELFAECGLDIDGVLAALAAQSDH"
BASE COUNT          532 a         1202 c         1305 g          581 t
ORIGIN      
        1 ccggttccgg cgccggccac cacgccggag gacctgctcg ccgatctcgg ctggagtgat
       61 gccgacctgg ggtcgccacc ggccatgccg gaaaagccgg tgactgcggc gccggcgccc
      121 agaccggcgg aagtcgcgga tctcgacttc ggcgacgatc tcgccgcctt cctgccgccg
      181 cgccagacga gcacgccggc gcaagcggca gcgaagccag ccgcggtcga tccggctacg
      241 gcggaactcg ccgacgatct gggcttctcg ttccagccgg tgtccgcgcc ggcgccggcg
      301 cccttgccgg tcgttcctgc cccggcggcg gcgcccccgg tgtcggcatc gacggccgat
      361 acccggggcg aggacgagtt cgtcgagacc aagctcgatc tggcactcgc ctacatcgac
      421 atggacgatc cgctcggtgc caagagcctg ctggaggaag tcctgcagga aggcggcagc
      481 agccagcgct cgcgtgccca ggcgctgctg gagcggctgg cgtcctgatc cgggtcggtc
      541 tttacagcgc gcgtatcccc atcggccggt gcatccaccg gccgatggcg tttctgggag
      601 gggccgaggt tcagccggcc gtcttgtcgc gctcgatcag cgcgtaggcc gagtggttgt
      661 ggatcgattc gaagttttcg gattcgatca cgtaggcggc gatgcgatca tcgtcgttga
      721 gccgtgcggc cacgtcgcgg accatgtcct cgacgaactt cggattctcg taggcgcgct
      781 cggtcacgta cttctcgtcc gggcgcttga gcaggccgta cagctcgcac gaggcttcct
      841 gctcgacgat gtcgatcagt tcctcgatcc acacgaaccc ggacagccgc gcgctgaccg
      901 tcacgtgcga gcgctgattg tgcgcgccgt agcgcgagat ttccttcgag cacgggcaga
      961 ggctggtgac cggcacgacc acgcgcagcc aggtggtcgc ctgctcgccg gcaagctcgc
     1021 cgatcagcgt cacctgatag tcgagcaggc tctgcacgcc cgagaccggt gccgtcttca
     1081 tgacgaagta cggaaagctc atctcgatgt ggccggtgcc ggcatcgagg cgggcgttca
     1141 tctcggtgag catctcgtgg aaggaggcga cgccgatctc gcgccccggg cggttcagca
     1201 cctcgacgaa gcgcgacatg tgcgtgccct tgaagtcatg cggcaggcgc acgtacatgt
     1261 tgaaggtcgc gaccgtgtgc tgctcgccgt cgctgcggtc gcgcacgcgc accggatggc
     1321 gcagatcctt gacgccgacc ttgtcgatcg gcaggcggcg ggtgtccggc gtgctctgga
     1381 tgtcggggat tgcagccggc tgctgctgct gcggtgcggg cacggcggtc ggcatgctca
     1441 tcgggatcgg actccgcagc ggacgcgctc gggcgtccgg tgaatgtcgg tcggcgcgcg
     1501 cgcaggcggc gcacgcggcg cctgccgggg ttgccgggca ggatcggcag actagcccgg
     1561 cgcaagtggt cgcagcaatt caggatattg cattgccgca atgccggttc gggtgtcgga
     1621 ctcagtgatc ggactgcgcg gccagcgcgg ccagcacgcc gtcgatgtcc agcccgcatt
     1681 cggcgaacag ctcaccgcgc tcgccgtgat cgaggaagcg atccggcagc ccgagcagct
     1741 tcagcgcggt gtgcacgccg tgtccggcca gacattcggc aaccgcgctg ccggctccgc
     1801 ccatcacggc gccgtcctcg atcgtgacca gacgctcgtg cgtgcgcgcg agttccagca
     1861 ccatcgcggt atcgagcggc ttgacgaagc gcatgttgac gacggtggca tcgagccgtt
     1921 cgccgacggc ctcggcgagc ggcagcatcg cgccgaaggc gagcagcgcg gtcccccggc
     1981 cctggcggcg gagttcggcc ctgccgatcg gcagcgccgt catcgtcgcg accggcgcga
     2041 ctcccggccc cttgccgcgc ggatagcgca cgcaggccgg cccgtcgtgc aggaagcccg
     2101 tgtacagcat ctgccggcat tcgtcctcgt cggccggcgc catgatcagc aggttcggca
     2161 cgcagcgcag atagcagaga tcgaaggaac cggcgtgggt cggaccgtcc gggccgacca
     2221 gcccggcgcg gtcgatcgcg aacagcacgg gcaggttctg gatcgcgaca tcgtggatca
     2281 gctggtcgta ggcgcgctgc aggaaggtcg aatagatcgc gaccacgggc ttgaggccgt
     2341 cgcaggccat gccggcggcc agcgtcaccg cgtgctgctc ggcgatgccg acgtcgaagt
     2401 agcgcgtcgg atagcgctcg gcgaactcga cgaggccgga gccttcgcgc atcgccggcg
     2461 tgatgccgac cagccgcggg tcctgggcgg ccatgtcgca gagccattcg ccgaacacct
     2521 gcgtgtaggt cggaccgccg ccagtcttgg ccggcgggat gccgctcgcc ggatcgaagc
     2581 tgccgacgcc gtggtaggtg accggctcgt tctcggcgag cttgtagccc ttgcccttgc
     2641 gagtcatcac gtgcagcagg cgcgggccct tctggtcgcg caggttctcg agcgtgcgca
     2701 ccagcgtcgg caggtcgtgg ccgtcgatcg ggccgaagta gttgaagccg agttcctcga
     2761 acagcgtgcc gggcgtgacc aggcccttgg cgtgctcctc ggcgcgccgg gccatctccc
     2821 acatgcttga gggcaggtgg gcgaggatgc gcttgccgcc ctcgcgcacc gtcgaataga
     2881 cgtggccgga cagcagccgg gtcaggtagc tcgacagtgc gccgacgttc ggcgagatcg
     2941 acatctcgtt gtcgttcagc acgaccagga tgtcgttctt caggtggccg gcgtggttca
     3001 gcgcctcgaa caccatgccg gcggtcatcg cgccgtcgcc gatcaccgcg accgcacgcc
     3061 ggcggctgcc ggcggcctcg gcggcgatcg ccatgccctg cgcagcactg atcgaggtgc
     3121 tcgaatgccc gacgccgaag gtgtcatacg ggctttcgca gcgcttcggg aagccggcga
     3181 gaccgtggcg gcggcgcatc gtgcccatga gggcgcgccg cccggtcagg atcttgtgcg
     3241 gatagctctg gtggccgaca tcccagacca ggcggtcctc gggcgtgtcg tacacgtagt
     3301 gcagcgcgat ggtcagctcg acggtgccga ggttggccga aaagtggccg ccggtgcggg
     3361 cgaccgactg gatcaggaat tcgcgcagct cgttggcgag ctggccgagg acgttctccg
     3421 gcagccggcg caggtcctgc ggggaatcga tggtgtcgag cagtggaaac acgggggtca
     3481 gggcagtcat gaaggcgtcc ggtgcgatcg gtatggggcc gcgggcgcgc gtcagtgcga
     3541 gcgttcgacg atgaatgccg cgagcgcacg cagcaccgcg gcgcgttcgc cgaagggctc
     3601 gagcgcgtgc atcgcgtcgt
//
LOCUS       DAOQJH010000232         3612 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_16242_length_3612_cov_4.216756, whole genome shotgun sequence.
ACCESSION   DAOQJH010000232 DAOQJH010000000
VERSION     DAOQJH010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3612
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16242_length_3612_cov_4.216756"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..753)
                     /locus_tag="PLQ18_06415"
     CDS             complement(<1..753)
                     /locus_tag="PLQ18_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006463434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNE01323.1"
                     /translation="MDFLNIPLPAVLAQLVVGLINGAFYALLSLGLAIIFGLLNIINF
                     AHGAQYMLGAFFAWFGVQYLGIDYWFALLLVPIAVGAIGMLLERFLLSKLYREDHLYG
                     LLLTFGLALIIEGVFVQLYGVSGKPYAVPEALRGALNLGFMFLPKYRAWVIVASVVVC
                     LGAWLVIERTKLGAYLRAGTENPRLLQAFGVNVPMMITLTYGFGVALAAFAGVLAAPI
                     FQVSPQMGADLIIVVFAVVVIGGMGSILGSILT"
     gene            complement(822..2036)
                     /locus_tag="PLQ18_06420"
     CDS             complement(822..2036)
                     /locus_tag="PLQ18_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNE01324.1"
                     /translation="MRHFRTVLSTAVAAALGSALAQAAISDDVIKIGVLTDMSGTYSD
                     LAGPGVVAAAEMAIADAGGKVAGKPIVMLKADHQNKADIAANKAREWNDVEKVDAYVD
                     LVTTSTALAVMEIAKNASKVVLVSGAGSSRITNEDCNPVTVHWTYDTYAMAKGTGSAM
                     VKEGGKTWFFLTADYAFGQNLQKDVSAVVEASGGKVLGAVKHPFPASDFSSFILQAQA
                     SGAQVIGLANAGADTTNAIKAANEFGIVAGGQKLAGLLVFISDVHSLGLPVAQGLTLT
                     TGFYWDYDDQTRAWSKRFFEKTGKMPTMVQAGVYSSLMHYFKAIEATGSDDGPTVIKQ
                     MKSGPVEDFFSRHGKIRDDGRLVHDMFLAEVKKPSESKAPWDYYKILRVIPGDEAYLP
                     LSESKCPLVRKS"
     gene            complement(2125..2847)
                     /locus_tag="PLQ18_06425"
     CDS             complement(2125..2847)
                     /locus_tag="PLQ18_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011763855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNE01325.1"
                     /translation="MTSAFQPDFEQLRVENLHAYYGESHILHGIALDVRRGELVTLLG
                     RNGAGKTTTLRAILGLTGRRSGAIHLNGQNIIDWPTHRIAHLGVGYCPEERGIFASLS
                     VEENLVLPPAVRSGGMSVEELYAMFPNLAERRKSQGTRLSGGEQQMLAMARILRTGAN
                     LLLLDEITEGLAPVIVQRLGEVLTLLKSRGYTVILVEQNFRFAAPLADRHYVIEHGRV
                     VDTIGRAELPAKMEALHQRLGV"
     gene            complement(2844..>3612)
                     /locus_tag="PLQ18_06430"
     CDS             complement(2844..>3612)
                     /locus_tag="PLQ18_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018315272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNE01326.1"
                     /translation="EHVLETRGLVKEFRGFVAVDHVDLQVRRGHIHALIGPNGAGKTT
                     VFNLLTKFLTPTRGTILFNGTDITAERPAQIARRGIVRSFQISAVFPHLTALENVRVA
                     LQRKLGTSFHFWKSPKTLDRLDARADELLDAVGLAQYRDTVTVEMPYGRKRALEIATT
                     LALEPELMLLDEPTQGMGHEDVDRVAELIRRVAEGRTILMVEHNLSVVSRLADRITVL
                     ARGATLAEGDYASVSQDPRVLEAYIGKAVDDTAGAHA"
BASE COUNT          579 a         1143 c         1315 g          575 t
ORIGIN      
        1 ggtgaggatc gagccgagga tcgagcccat gccgccgatc acgaccaccg cgaacacgac
       61 gatgatcagg tcggcgccca tctgcgggct gacctggaag atcggcgcgg cgagcacgcc
      121 ggcgaaggcc gcgagcgcga cgccgaagcc gtaggtgagc gtgatcatca tcggcacgtt
      181 gacgccgaag gcctgcagca ggcgcgggtt ctcggtgccg gcgcgcaggt aggcaccgag
      241 cttggtgcgc tcgatcacca gccaggcgcc gagacagacg acgaccgagg cgacgatgac
      301 ccaggcgcgg tacttgggca ggaacatgaa gccgagattg agcgccccgc gcagcgcctc
      361 gggcacggcg tagggcttgc ccgagacgcc gtagagctgc acgaacacgc cctcgatgat
      421 cagcgccagg ccgaaggtca gcagcagccc gtacaggtga tcctcgcggt agagcttcga
      481 gagcaggaag cgctccagca gcatgccgat ggcgccgacc gcgatcggca cgagcagcag
      541 cgcgaaccag tagtcgatgc cgaggtactg cacgccgaac caggcgaaga aggcgccgag
      601 catgtactgc gcgccgtgcg cgaagttgat gatgttcagc agcccgaaga tgatcgcgag
      661 gccgagcgac agcagcgcgt agaaggcgcc gttgatcagg ccgacgacga gctgcgcgag
      721 cacggccggc agcgggatgt tcaggaagtc catgacgagc ctccggcgcg gggttcaggc
      781 gcgcacaccg gcgccgccgg ccggtgcgcg cacggagggg cttacgactt cctgaccagc
      841 gggcacttgc tctcggaaag cggcaggtag gcctcgtcgc cggggatcac gcgcaggatc
      901 ttgtagtagt cccacggcgc cttcgactcg gacggcttct tgacctcggc gaggaacatg
      961 tcgtgcacga ggcggccgtc gtcgcggatc ttgccgtggc gcgagaagaa gtcctcgacc
     1021 gggccggact tcatctgctt gatgacggtc gggccgtcat cggagccggt ggcctcgatc
     1081 gccttgaagt aatgcatcag cgacgagtac acgccggcct gcaccatggt cggcatcttg
     1141 ccggtcttct cgaagaagcg cttcgaccag gcgcgggtct ggtcgtcgta gtcccagtag
     1201 aagccggtgg tcagcgtcag gccctgcgcg accggcaggc cgaggctgtg cacgtcggag
     1261 atgaacacca gcaggccggc gagcttctgg ccgccggcga cgatgccgaa ctcgttggcg
     1321 gccttgatgg cgttggtggt gtcggcaccg gcgttggcaa ggccgatgac ctgcgcgccc
     1381 gaggcctgcg cctgcaggat gaaggagctg aagtcgctcg ccgggaacgg atgcttgacc
     1441 gcgccgagca ccttgccgcc gctcgcctcg accacggcac tgacgtcctt ctgcaggttc
     1501 tggccgaagg cgtagtcggc ggtcaggaag aaccaggtct tgccgccctc cttgaccatc
     1561 gccgagccgg tgcccttggc catcgcgtag gtgtcgtagg tccagtgcac ggtgacgggg
     1621 ttgcagtcct cgttggtgat gcgcgaggag ccggccccgg agaccagcac gaccttgctc
     1681 gcgttcttgg cgatctccat gaccgcgagc gcggtgctgg tggtgaccag atcgacgtag
     1741 gcatcgacct tctcgacatc gttccactcg cgcgccttgt tggcggcgat gtcggccttg
     1801 ttctggtgat cagccttcag catcacgatc ggcttgccgg cgaccttgcc gccggcatcg
     1861 gcgatggcca tctcggcggc ggcgacgacg ccgggaccgg caagatccga gtaggtgccg
     1921 gacatgtcgg tgagcacgcc gatcttgatc acgtcatcgg agatcgcggc ctgtgcgagg
     1981 gcgctgccca gggcagccgc gacggcggtg gacagcacgg tgcggaagtg acgcatcggg
     2041 gggttctcct ctgggtttcc ggacggaaag ggctgcgggt ggttcttgtt ggaatggcgc
     2101 gttccggcgc agggcacgag cggttcagac gccgaggcgc tggtgcaggg cttccatctt
     2161 cgcgggcagc tcggcgcgcc cgatggtgtc aacgacgcga ccgtgctcga tcacgtaatg
     2221 ccggtcggcg agcggcgcgg cgaagcggaa gttctgctcg acgaggatca ccgtgtagcc
     2281 gcgcgacttc agcagcgtca gcacctcgcc gaggcgctgg acgatgaccg gggcgaggcc
     2341 ttcggtgatc tcgtcgagca gcagcaggtt ggcgccggtg cgcaggatgc gcgccatcgc
     2401 cagcatctgc tgctcgccgc cggagaggcg cgtgccctgg cttttgcgcc gctcggcgag
     2461 gttcgggaac atcgcgtaga gctcctcgac gctcatgccg cccgagcgca cggccggcgg
     2521 cagcacgagg ttctcctcga ccgagaggct cgcgaagatg ccgcgctcct ccgggcagta
     2581 gccgacgccg aggtgggcga tgcggtgcgt cggccagtcg atgatgttct ggccgttgag
     2641 gtggatggcg ccgctgcgcc ggccggtgag gccgaggatc gcgcgcagcg tcgtggtctt
     2701 gccggcgccg ttgcgcccga gcagcgtcac cagttcgccg cggcgcacgt cgagcgcgat
     2761 gccgtgcagg atgtgcgatt cgccgtaata ggcatgcagg ttctcgacgc gcagctgctc
     2821 gaagtcgggc tggaaggcgc tggtcatgcg tgggctcccg cggtgtcatc gacggccttg
     2881 ccgatgtagg cctccagcac gcgcgggtcc tgcgagacgc tggcgtagtc gccctcggcg
     2941 agcgtggcgc cgcgcgcgag cacggtgatg cgatcggcaa gccgcgacac cacgctcagg
     3001 ttgtgctcga ccatcaggat ggtgcgcccc tcggcgacgc ggcggatcag ctcggcgacg
     3061 cgatcgacat cctcgtggcc catgccctgg gtcggctcgt cgagcagcat cagctcgggt
     3121 tcgagcgcga gcgtggtcgc gatctccagc gcgcgcttgc gcccgtacgg catctcgacc
     3181 gtcacggtgt cgcggtactg cgccagcccc acggcatcga gcagttcatc ggcgcgggca
     3241 tcgaggcgat cgagcgtctt cggcgacttc cagaaatgaa aggaagtgcc gagcttgcgc
     3301 tgcagcgcga cgcgcacgtt ctcgagcgcg gtgagatgcg ggaacaccgc cgagatctgg
     3361 aacgagcgca cgatgccgcg ccgtgcgatc tgcgccggcc gctcggcggt gatgtcggtg
     3421 ccgttgaaca ggatcgtgcc gcgcgtcggc gtcaggaact tggtcagcag gttgaagacg
     3481 gtggtcttgc cggcgccgtt cgggccgatc agcgcgtgga tgtggccgcg gcgcacctgc
     3541 aggtcgacgt gatcgacggc gacgaagccg cggaactcct tcacgagccc gcgcgtttcg
     3601 agtacgtgct ct
//
LOCUS       DAOQJH010000233         3609 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_16271_length_3609_cov_3.879572, whole genome shotgun sequence.
ACCESSION   DAOQJH010000233 DAOQJH010000000
VERSION     DAOQJH010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3609)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3609)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3609
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16271_length_3609_cov_3.879572"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..798)
                     /locus_tag="PLQ18_06435"
     CDS             complement(<1..798)
                     /locus_tag="PLQ18_06435"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dyp-type peroxidase"
                     /protein_id="HNE01327.1"
                     /translation="MSTPQPGILAPIPAASRHLFFHVNACHDAPRALQALRGLVDGFA
                     VVVGIGAELAAGLDAPIPGLRPFPSLPGARVPIPYVPYALWCWLRGSDRGELFHTGRE
                     IENALAGVFTLELAIDCFCHRDSRDLTGYEDGTENPVDEAALAAAIVSHEGDGLDGGS
                     FASVQQWLHDIERFQRLSPGEQDDTVGRSRIDNEELEDAPDSAHVKRTAQEDFDPPAF
                     MVRRSMPWTAERRGGLVFLAFGHSPDAFEAVLRRMSGAEDGIHDALFN"
     gene            complement(863..1327)
                     /locus_tag="PLQ18_06440"
     CDS             complement(863..1327)
                     /locus_tag="PLQ18_06440"
                     /inference="COORDINATES: protein motif:HMM:NF040030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6231 family protein"
                     /protein_id="HNE01328.1"
                     /translation="MPISPIDLPAGACGPFANECSDWLRACAPGRLLLLGDAGDLTLP
                     PGTEVDARADASALAADARYDAVLIRGLPELLDRRAGAALLARARDRLAPECLAVLPR
                     PCWDEGAVLGLGYTLRARAPAALLGHFAIASYKSVPDWFNSKFWAHPERWQP"
     gene            1442..2143
                     /gene="mobB"
                     /locus_tag="PLQ18_06445"
     CDS             1442..2143
                     /gene="mobB"
                     /locus_tag="PLQ18_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005224408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HNE01329.1"
                     /translation="MMQGRGVIATGNGVPPVDAGLAAPDAAVAACPLPVLGIVGPSGA
                     GKTALLCRLLPELAAAGLRVGVIKQASARFDLDEPGRDSDRLRRAGLARTLVAAVGQT
                     ALIEERDPACQPGDCELADALGWLDVGALDLVLVEGFARAALPKLEVLRADGPKRHQY
                     RHDAWVRVLLADHEPEPESESESEPAAAAFRLPRLDLADATAIAGWLCAYREEFLARR
                     AGRTGGAARSGELAR"
     gene            2140..3450
                     /locus_tag="PLQ18_06450"
     CDS             2140..3450
                     /locus_tag="PLQ18_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008648395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HNE01330.1"
                     /translation="MSLSCSPGSDAIRAQASCCDDHDPHSLPVPVALARIQTAVQPVT
                     ASEQVALRAALGRVLAADVVSPIDVPAHVNSAMDGYAVRAADLPEAGTVTLAVVGTAF
                     AGAAWDGRLASGQAVRIMTGAVLPEGADCVVMQEQTEREQGGDQEGGQDRVRIGRGHR
                     AGQNIRLAGEDLKTGETVLAAGRRVQPADLGVLASLGVAEVRVRRRLRVAFFSTGDEL
                     RSIGEPLAAGCVYDSNRYSLYGMLSRLGVELIDMGVVRDDREALQAAFREAAACADAV
                     ITSGGVSVGEADFVKDTLLSLGRIDFWKLAMKPGRPLAFGRLGEAVFFGLPGNPVSVV
                     VTFYQFVQPALRRMMGMNAVQPVTLQVPTLARLKKRPGRTEFQRGVLEQRDDGSMAVR
                     PTGDQGSGILSSVSRADCFIVLAADSSGVEAGDTVTVQPFEGLF"
BASE COUNT          470 a         1276 c         1356 g          507 t
ORIGIN      
        1 gttgaacagc gcgtcatgga tgccgtcctc ggcgccgctc atgcgccgca ggaccgcctc
       61 gaaggcatcg gggctgtggc cgaaggcgag gaacaccagc ccgccgcggc gttcggcggt
      121 ccatggcatc gagcggcgca ccatgaaggc cggcggatcg aagtcctcct gcgcggtgcg
      181 cttgacgtgg gccgagtccg gcgcgtcctc gagttcctcg ttgtcgatgc ggctgcgccc
      241 gaccgtgtcg tcctgctcgc ccggcgacag gcgctggaag cgctcgatgt cgtgcagcca
      301 ctgctgcacc gacgcgaagc tgccgccatc gaggccgtcg ccctcgtggc tgacgatcgc
      361 ggcggcgagc gcggcctcat cgaccgggtt ctcggtgccg tcctcgtaac cggtcaggtc
      421 gcggctgtcg cggtggcaga agcagtcgat cgccagttcc agcgtgaaca cgccggccag
      481 cgcgttctcg atctcgcgcc cggtgtggaa cagctcgccg cggtcgctgc cgcgcagcca
      541 gcaccacagc gcatacggca cgtacgggat cggcacgcgc gcgccgggga ggctcgggaa
      601 cggccgtagg cccgggatcg gcgcatcgag gccggcggcg agttcggcgc cgatgccgac
      661 gacgacggca aacccgtcga ccagcccgcg cagcgcctgc agcgcccgcg gtgcgtcgtg
      721 acaggcgttg acgtggaaga acagatgccg cgaggcggcc gggatcgggg cgaggatgcc
      781 gggttgcggg gtactcatca cgggggaact ccgtcaggac gaatcggaag ggaatcagca
      841 gcgcagcagt cgaggcagcc gctcagggct gccagcgttc cgggtgcgcc cagaacttgc
      901 tgttgaacca gtcgggcacg ctcttgtagc tcgcgatcgc gaaatgcccg agcagcgccg
      961 ccggtgcgcg ggcgcgcagc gtgtagccga ggccgagcac ggcgccctcg tcccagcacg
     1021 gccggggcag cacggccagg cattccggcg ccagccggtc gcgggcgcgg gcgagcaggg
     1081 cggctccggc gcgccggtcg agcagttcgg gcaggccgcg gatcagcacc gcgtcgtagc
     1141 gggcatcggc ggcgagcgcg ctggcatcgg cacgggcatc gacctccgtg cccggtggca
     1201 gcgtcaggtc gccggcatcg ccgagcagca gcagccggcc cggcgcgcag gcccgcagcc
     1261 agtcactgca ttcgttcgca aacggcccgc aggccccggc aggcaggtcg atcggactta
     1321 tcggcacatc ggcatactcc gcgcggaaac tggcagtgac ggggacgatc gcggacactg
     1381 cgcggtcacg cgagagtacg gcagcgccgg tcgcgcggtc gatcggcagc ggaggatgca
     1441 gatgatgcag ggcaggggcg tgatcgccac cggcaatggt gtgccgccgg tggatgcggg
     1501 cctggcagcg cccgatgcgg ccgtcgcggc ctgtccgctg ccggtgctcg gcatcgtcgg
     1561 accgagcggg gccggcaaga cggcgctgct ctgccggctg ctgccggagc ttgccgccgc
     1621 cggtctgcgc gtcggcgtca tcaagcaggc ctcggcgcgc ttcgatctcg atgagcccgg
     1681 ccgcgacagc gatcgcctgc gccgcgccgg actggcgcgc acgctggtgg ccgccgtcgg
     1741 ccagaccgcg ctgatcgagg agcgcgaccc ggcctgtcag cctggcgact gtgaactcgc
     1801 cgatgcgctc ggctggctcg atgtcggggc gctcgatctg gtgctggtcg agggcttcgc
     1861 gcgcgcggcg ctgccgaagc tcgaagtgct gcgcgccgac ggcccgaagc gccaccagta
     1921 ccggcacgat gcgtgggtgc gcgtgctgct cgccgatcac gagcctgaac ctgaatccga
     1981 atctgaatcc gaacccgcgg ccgccgcctt ccgcctgcca cggctcgatc tcgccgatgc
     2041 cacggcgatc gccggctggc tgtgcgcgta tcgcgaggaa tttcttgccc gccgcgccgg
     2101 ccgcaccggc ggcgcggccc gatccggaga gctggcccga tgagtctgtc ctgtagtccc
     2161 ggcagcgatg ccatccgcgc gcaggcttcg tgctgcgacg atcacgaccc gcactcgctg
     2221 ccggtgccgg tggcgctcgc gcgcatccag accgccgtgc agccggtcac tgccagcgaa
     2281 caggtggcgc tgcgcgccgc gctcgggcgc gtgctcgccg ccgatgtggt ttcgccgatc
     2341 gacgtgcccg cgcacgtgaa ctcggcgatg gacggctacg ccgtgcgcgc cgccgacctg
     2401 ccggaggcag gcaccgtgac gctcgcggtg gtcggcaccg cgttcgccgg tgcggcctgg
     2461 gacggcaggc tcgccagcgg ccaggccgtg cgcatcatga ccggcgccgt gctgcccgag
     2521 ggcgccgact gcgtcgtgat gcaggagcag accgagcgcg agcagggtgg cgatcaggag
     2581 ggcggccagg atcgcgtgcg catcggccgc ggtcaccgcg ccggccagaa catccgcctc
     2641 gccggcgagg acttgaagac cggcgagacc gtgctcgccg ccggccggcg cgtgcagccg
     2701 gccgatctcg gcgtgctcgc ctcgctcggc gtcgccgagg tgcgcgtgcg ccggcgcctg
     2761 cgcgtcgcct tcttctcgac cggcgacgaa ctgcgctcga tcggcgagcc gctggccgcc
     2821 ggctgcgtct acgacagcaa ccgctattcg ctgtacggca tgctgagccg cctcggcgtc
     2881 gagctgatcg acatgggtgt cgtgcgcgat gaccgcgagg cactgcaggc ggccttccgc
     2941 gaggccgcgg cctgcgccga tgccgtgatc acctccggcg gcgtgtcggt cggcgaggcc
     3001 gacttcgtca aggacacgct gctgagcctc gggcgcatcg acttctggaa gctggcgatg
     3061 aagcccggcc ggccgctggc cttcgggcgc ctcggcgagg ccgtgttctt cggcctgccc
     3121 ggcaacccgg tgtcggtggt cgtgaccttc taccagttcg tgcagccggc actgcggcgg
     3181 atgatgggca tgaacgccgt gcagccggtg acgctgcagg tgccgacgct cgcgcgcctg
     3241 aagaagcgcc ccgggcgcac cgagttccag cgcggcgtgc tggagcagcg cgacgacggc
     3301 tcgatggccg tgcgcccgac cggcgaccag ggctcgggca tcctcagttc ggtcagccgc
     3361 gccgactgct tcatcgtgct cgccgccgac agcagcggcg tcgaggccgg cgacacggtg
     3421 acggtgcagc ccttcgaagg gctgttctga tggtgccggc cgccgccgga tgcgcaggcc
     3481 ggcggccgtg gtgagcggtc tcgggctgct cgccgtcggc ctcggcgccg ccgtcggtgc
     3541 ctggctgcgc tggctgctcg ggctgtggct gaacccgctg ttcccgccgg tgccgctcgg
     3601 cacgctggc
//
LOCUS       DAOQJH010000234         3601 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_16336_length_3601_cov_4.144670, whole genome shotgun sequence.
ACCESSION   DAOQJH010000234 DAOQJH010000000
VERSION     DAOQJH010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3601
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16336_length_3601_cov_4.144670"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..932)
                     /locus_tag="PLQ18_06455"
     CDS             complement(<1..932)
                     /locus_tag="PLQ18_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015004207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HNE01331.1"
                     /translation="MERSPRKVRKLLVANRSEIAIRAMRAAAELGIATVAIYSEEDRF
                     ALHRFKADESYRVGQGRKPIQAYLDIDDILRVAKEAGADAIHPGYGFLSENPDFAEAC
                     AQNGILFIGPSPEVMRTLGNKVAARNAAVAAGVPVMPATGPLPRDLDEVKKMAAAIGF
                     PFMLKASWGGGGRGMRVIETEADLGPQIEVARREAAAAFGNDEVYLEKLVRRARHVEV
                     QILGDRHGNIVHLFERDCTVQRRNQKVVERAPAPYLDEATRSELCESALRLARQVGYT
                     AAGTVEFLMDADTGRFYFIEVNPRIQVEHTVTEQV"
     gene            1118..1660
                     /locus_tag="PLQ18_06460"
     CDS             1118..1660
                     /locus_tag="PLQ18_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015280306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HNE01332.1"
                     /translation="MPIRSFENLVPHIAASAWIDDSAVVIGDVQIGAEASIWPMCVVR
                     GDIQSIRIGARSNVQDGTVIHVTHDSRFCPGGRATTVGEDVTIGHQALLHACTIGDRC
                     LIGMGAIVMDGAGIGAGAMLGAGCVVPPGKQLAGGFLYVGSPARQQRPLSEREIEFLG
                     YSAENYVKLAARHRAGSRSV"
     gene            complement(1768..2196)
                     /locus_tag="PLQ18_06465"
     CDS             complement(1768..2196)
                     /locus_tag="PLQ18_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE01333.1"
                     /translation="MKTLLIVEDNPQDELLIRRALRKAALANPVDVVRDGQQALDWLW
                     REGEFAGYPDTGLPAVVLLDLSLPRVSGLDVLERLRADARTRLQPIVVLTSSDEERDR
                     LTSYAHGANAFVRKPLDFAEFAEAVARIGVFWLMTNVGPA"
     gene            complement(2193..>3601)
                     /locus_tag="PLQ18_06470"
     CDS             complement(2193..>3601)
                     /locus_tag="PLQ18_06470"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNE01334.1"
                     /translation="EYTGQPEAAQLGSGWLEHVYPDDRERLVAAWTAAVAGGHDFSVE
                     FRIRRAADGAWRWFDTRALPLRDAQGRIVRWFGTNTDVQATRETAEALRASEARFRAL
                     LDQAADAIYVHDADGRLCDVNHRACETLGLPRATLLTMNVADVDPALPAAGLAFWHTL
                     TPGESLTLRSRHRRGDGSDFPVELSVGAVEIGGERRLLALARDVGERERAEAEIHELN
                     ASLEQRVAERTAELTAANRELDAFAYAVSHDLRAPLRAMSGFSTALLEDCAGQLDADG
                     RAYLDQIIAGSRRMGELIDGLLALSRSTRGELRRERVDVTAIMARVREELEADEPQRR
                     VHWQVEPDLHVWGDAGMLDAVFANLAGNAWKYTARTADPVIRVQAAEAAAGFLCVCVS
                     DNGAGFDPRHAGKLFLPFQRLHRQDEFPGLGIGLATAQRIVHRHGGTIAASAAPGLGA
                     RFCITLPGPQVASEEPES"
BASE COUNT          536 a         1213 c         1293 g          559 t
ORIGIN      
        1 acctgctcgg tgacggtgtg ctcgacctgg atgcgcgggt tgacctcgat gaaatagaac
       61 ctgccggtgt cggcatccat caggaattcg acggtgccgg cggccgtgta gccgacctgg
      121 cgcgcgaggc gcagcgccga ttcgcagagt tcgctgcgcg tggcctcgtc gagatacggc
      181 gccggggcgc gctcgaccac cttctggttg cgccgctgca ccgtgcagtc gcgctcgaac
      241 aggtggacga tgttgccgtg ccggtcgccg aggatctgca cctcgacgtg gcgcgcgcgg
      301 cgcacgagct tttcgagata gacctcgtca ttgccgaagg ccgcggcggc ctcgcgccgg
      361 gcgacctcga tctgcggacc gagatcggcc tcggtctcga tcacgcgcat gccgcgaccg
      421 ccgccgcccc agctcgcctt gagcatgaac gggaagccga tggcggcagc catcttcttc
      481 acttcgtcga gatcgcgcgg cagcgggccg gtggccggca tcaccggcac gccggcggcg
      541 accgcggcgt tgcgcgcggc gaccttgttg ccgagcgtgc gcatgacctc gggcgaggga
      601 ccgatgaaca ggatgccgtt ctgcgcgcag gcttcggcga agtcggggtt ctcggacagg
      661 aagccgtagc cgggatggat ggcgtcggca ccggcctctt tggcgacgcg caggatgtcg
      721 tcgatgtcga gataggcctg gatgggtttt cggccctgac cgacgcggta actctcgtcg
      781 gccttgaagc ggtgcagcgc gaaacggtct tcctccgaat agatcgcgac ggtggcgatg
      841 ccgagctcgg cggcggcacg catcgcacgg atggcgatct cgctgcggtt ggccaccagc
      901 agcttgcgga ctttgcgagg actgcgctcc atcctgtcta cctctacgac aatcacatga
      961 ataggcagtg ttaatggttg aaattcagac cattgtctat ccacattagc ggaattccac
     1021 acatacgaca atgcgcattg tgcgattttc cgcacatgca ctttcatagt gcaaaataat
     1081 gttcgtattc cgactgcctg ccccggagaa ttgcccgatg ccgatccgca gtttcgaaaa
     1141 tctcgtcccg catatcgccg ccagcgcctg gatcgacgac agcgccgtcg tcatcggcga
     1201 cgtgcagatc ggcgccgaag cctcgatctg gccgatgtgc gtggtgcgcg gcgacatcca
     1261 gtcgatccgc atcggcgcgc gcagcaacgt gcaggacggg accgtcatcc acgtcacgca
     1321 cgattcgcgt ttctgtcccg gagggcgcgc cacgaccgtg ggcgaggacg tgaccatcgg
     1381 ccatcaggcg ctgctgcacg cctgcacgat tggtgatcgc tgcctgatcg gcatgggggc
     1441 gatcgtcatg gacggcgccg ggatcggcgc cggcgcgatg ctcggcgccg gctgcgtggt
     1501 gccgccgggc aagcagctcg caggcggctt cctgtacgtc ggcagcccgg cgcggcagca
     1561 gcgcccgctc agcgagcgcg agatcgagtt cctcggctat tcggccgaaa actacgtgaa
     1621 gctcgcggcc cggcaccggg ccggcagccg gagcgtgtga gcgcctgcgg aaaaaccctt
     1681 gcaaggcatt gagcggacgg tcgccttttc ccccgggcgc cgcacgcggc gatgcgcccg
     1741 gatgccgctg cgcgctgcgg cgccccttca ggccgggccg acgttggtca tcagccagaa
     1801 cacgccgatg cgcgcgaccg cctcggcgaa ctcggcgaag tcgaggggct tgcgcacgaa
     1861 ggcgttggca ccgtgggcgt agctggtgag gcggtcacgc tcctcgtccg aggaggtcag
     1921 gaccacgatc ggctgcaggc gcgtgcgcgc atcggcgcgc aggcgttcga gcacgtcgag
     1981 gccgctgacg cgcggcaggc tcaggtcgag cagcacgacg gccggcaggc cggtgtcggg
     2041 gtagccggcg aactcgcctt cgcgccacag ccagtcgagc gcctgctggc cgtcgcgcac
     2101 cacatcgacg ggattggcga gcgcggcctt gcgcagggcg cggcggatca gcagctcgtc
     2161 ctgcgggttg tcttcgacga tcagcaaggt cttcatgatt cgggctcttc ggaggccacc
     2221 tgcggaccgg gcagcgtgat gcagaaacgg gcgccgagac cgggcgcggc gcttgcggcg
     2281 atcgtgccgc cgtggcggtg cacgatgcgc tgcgcggttg caaggccgat gccgaggccg
     2341 ggaaactcgt cctggcgatg caggcgctgg aacggcagga acagcttgcc ggcgtgacgc
     2401 gggtcgaagc cggcgccgtt gtcgctgacg cacacgcaca ggaagccggc agcggcctct
     2461 gccgcctgca cgcggatgac gggatcggcg gtgcgggcgg tgtacttcca ggcgttgccg
     2521 gcgaggttcg cgaacaccgc atcgagcatg ccggcatcgc cccagacgtg caggtccggc
     2581 tcgacctgcc agtgcacgcg ccgctgcggt tcgtccgctt cgagttcctc gcgcacgcgc
     2641 gccatgatcg cggtcacatc gacgcgctcg cggcgcagct cgccgcgcgt gctgcgcgac
     2701 agcgccagca ggccgtcgat cagctcgccc atgcgccggc tgcccgcgat gatctggtcg
     2761 agatacgcgc ggccgtcggc atcgagctgc ccggcgcagt cctccagcag cgccgtgctg
     2821 aagcccgaca tcgcgcgcag cggcgcgcgc aggtcgtgcg agacggcgta ggcgaaggcg
     2881 tcgagttcgc ggttggcggc ggtcagctcg gcggtgcgct cggcgacgcg ctgctccagc
     2941 gaggcgttca gctcgtgaat ctcggcctcg gcgcgctcgc gctcgccgac gtcgcgcgcg
     3001 agcgcgagca gccggcgctc gccgccgatc tcgacggcgc cgacgctgag ctcgaccgga
     3061 aaatcactgc cgtcaccgcg gcggtggcgg ctgcgcagtg tcagggactc gccgggcgtg
     3121 agcgtgtgcc agaacgccag acccgcggcc ggcaaggccg gatcgacatc ggcgacgttc
     3181 atcgtcagca gcgtcgcacg cggcaggccg agggtctcgc aggcacggtg gttcacgtcg
     3241 cacaggcgcc cgtcggcatc gtgcacgtag atggcatcgg cggcctgatc gagcagcgcc
     3301 cggaagcgcg cctcgctcgc gcgcagcgcc tcggcggtct cgcgcgtggc ctgcacgtcg
     3361 gtgttggtgc cgaaccagcg cacgatgcgg ccctgcgcat cgcgcagcgg cagcgcgcgc
     3421 gtgtcgaacc agcgccaggc gccgtcggcg gcgcggcgga tgcggaattc gacgctgaaa
     3481 tcgtggccgc cggcgaccgc cgccgtccac gccgccacca gccgctcgcg gtcatccggg
     3541 tagacgtgtt cgagccagcc gctgccgagc tgcgcggcct cgggctggcc ggtgtattcg
     3601 a
//
LOCUS       DAOQJH010000235         3599 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_16362_length_3599_cov_3.161682, whole genome shotgun sequence.
ACCESSION   DAOQJH010000235 DAOQJH010000000
VERSION     DAOQJH010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3599)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3599)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3599
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16362_length_3599_cov_3.161682"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..277
                     /locus_tag="PLQ18_06475"
     CDS             <1..277
                     /locus_tag="PLQ18_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006216495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutathione S-transferase C-terminal
                     domain-containing protein"
                     /protein_id="HNE01335.1"
                     /translation="CNADIARLQALIRESRLLHGADGPWLFGEFSAADAMLAPVAWRF
                     TTYDVPHDDIVGAWLSTVLECEAMRAWANDARTETEIIAADEAGIPV"
     gene            complement(281..1270)
                     /locus_tag="PLQ18_06480"
     CDS             complement(281..1270)
                     /locus_tag="PLQ18_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011553829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HNE01336.1"
                     /translation="MLLPHLRRLLRDRFLLGMLLAVGLAVLTPQFGRSGGTLHLDALS
                     NAGIFAVFFLHGIGLSMQSLRAGLLNWRLHTLVQAHTFLVFPLLWLALDALFGAWVPQ
                     GLMLGFWYLCALPSTISSSVAMTALGRGNVPGAIFNATLSSLLGIVLTPLLVSLMVAH
                     GGEGGRGPGEAILNIAGLLLAPFVLGQLLRPWLGAWFAERKRWTGLIDRGVILMLVYG
                     SFCDATAGGLWSDYGAGVLATAFAGSALLLAIVLGLSRRAARLFGFSVEDEVAAVFCG
                     SKKTLASGMPMAKLLFGASPALGLVVLPILLYHPLQLFVCSVMAERYAARPAR"
     gene            1371..2207
                     /locus_tag="PLQ18_06485"
     CDS             1371..2207
                     /locus_tag="PLQ18_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003147081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNE01337.1"
                     /translation="MSNSGHLAPALTPRPADAPERSVPALERLPRAVYSRAESLPAGS
                     WVRPHRHDWVQLSWALSGVLEVRTDTGCWLAPPQRAVWIPPGTLHEVNASARAEMRSL
                     YLDASRTAWAPPRCRVLAVSPLVRELILALSARPLEYDPDGPAGRLAEVLLDAIAELP
                     ELDFGLPLPADARLRRLCEALQAEPADARTLPEWAARCGLSERTLTRRFRRETGLSFA
                     DWRRRLRLLLALGALEAGRSVTAVALDSGYDSLSAFIAAFRNEFGTTPKALFAPAPVN
                     DG"
     gene            2280..2810
                     /locus_tag="PLQ18_06490"
     CDS             2280..2810
                     /locus_tag="PLQ18_06490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE01338.1"
                     /translation="MSTDPLLTSDFAEALLATQTGPQAPATLRFDATRTGLAFGGTVP
                     AVRVYAFGPASLARHWHPGFPTPAQLEYAIAAVEDELMRVHRHCGPPPSLASAVCPDP
                     EPRALAASLGLPGSGRVQLLREAVEHGFGRLAACAEGRPSDSGGLPQDTNGMALLLIL
                     RELMHHLPLAALELPA"
     gene            2896..3225
                     /locus_tag="PLQ18_06495"
     CDS             2896..3225
                     /locus_tag="PLQ18_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017571550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HNE01339.1"
                     /translation="MITRIRQGRTRPGQAGEYEPLLLEHVFPEIAARALSGWPGYELL
                     RRERSHEVEFVTIMHFDDQAALHTFDHLDGSAEGVPPEARTLLAHFDSLSSHYRTVAV
                     RAGGHTT"
     gene            3222..>3599
                     /locus_tag="PLQ18_06500"
     CDS             3222..>3599
                     /locus_tag="PLQ18_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017301323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNE01340.1"
                     /translation="MNAVANRLYLRLARPGDADAIAGLMTLLGYPVEAGETAGRLERL
                     LRHPDAAVLVGGLEDGPVQGCLALHFIPQLGLAGDFCRIAYFSVAPAAQGQGLGRALE
                     AEATRLAQQRGCDRIEVHSHERRH"
BASE COUNT          549 a         1360 c         1205 g          485 t
ORIGIN      
        1 ctgtaacgcc gacatcgcgc ggctgcaggc gctgatccgc gaaagccggc tgctgcacgg
       61 cgccgacggc ccttggctgt tcggcgagtt ctcggccgcc gatgcgatgc tcgcgccggt
      121 cgcctggcgc ttcacgacct acgacgtgcc gcacgacgac atcgtcggtg cctggctgtc
      181 gacggtgctc gaatgcgagg cgatgcgcgc ctgggcgaac gacgcgcgca ccgaaaccga
      241 gatcatcgcc gccgacgagg ccggcatacc ggtctgaagc tcaacgtgcc ggccgggccg
      301 cgtagcgttc ggccatcacc gagcagacga acagctgcag cgggtgatag agcaggatcg
      361 gcagcaccac cagcccgagc gcggggctgg cgccgaacag cagcttcgcc atcggcatgc
      421 ccgaggccag cgtcttcttc gagccgcaga acaccgccgc cacctcgtcc tcgaccgaaa
      481 agccgaacag gcgtgcagcg cgccggctca gccccagcac gatcgccagc agcagcgcac
      541 tgccggcaaa cgccgtcgcg agcacgccgg cgccgtagtc cgaccacagc ccgccggccg
      601 tcgcgtcgca gaacgagccg tagaccagca tcaggatcac gccgcggtcg atcaggcccg
      661 tccagcgctt gcgctctgca aaccaggcgc cgagccacgg cctcagcagc tgcccgagca
      721 cgaagggcgc gagcagcagg ccggcgatgt tcaggatcgc ctcgccgggg ccgcgcccgc
      781 cctccccgcc gtgagcgacc atcaggctga ccagcagcgg cgtcagcacg atgccgagca
      841 ggctcgacag cgtggcgttg aagatcgccc ccggcacgtt gccgcgcccg agcgcggtca
      901 tcgccaccga cgaggagatc gtcgagggca gcgcgcacag ataccagaag ccgagcatca
      961 gcccctgcgg cacccaggcg ccgaacagcg catcgagtgc tagccacagc agcgggaaga
     1021 cgaggaaggt atgcgcctgc accagcgtgt gcagccgcca gttcagcaag cccgcgcgca
     1081 ggctctgcat cgacagcccg atgccgtgca ggaagaacac cgcaaagatg ccggcattcg
     1141 agagcgcatc gaggtgcagc gtgccgccgc tgcgcccgaa ttgcggcgtc agcaccgcga
     1201 gtccgacggc gagcagcatg ccgagcagga aacggtcgcg cagcaggcga cgcaggtgag
     1261 gcagcagcat ggcggaatct ccgacaccgg tacggtcgcc gggaacgcga ccgtctgccg
     1321 ggcatactgc gcaggaacgg cctgtccggt cgtcgccgcg gggtcaatcg atgtcgaatt
     1381 ccggccatct cgcgcccgcc ctgacgccac ggcccgccga tgcgcccgag cgcagcgtgc
     1441 cggcgctcga acgcctgccg cgcgccgtct actcgcgcgc cgaatcgctg ccggccggct
     1501 cctgggtgcg tccgcaccgc catgactggg tgcagttgtc gtgggcgctc agcggcgtgc
     1561 tcgaagtgcg caccgacacc ggctgctggc tggccccgcc gcagcgcgcg gtgtggatcc
     1621 cgcccggcac gctgcacgag gtcaacgcct cggcgcgtgc cgagatgcgc agcctttatc
     1681 ttgatgcgtc acgcactgcc tgggccccgc cgcgctgccg ggtgctggcc gtgagcccgc
     1741 tggtgcgcga gctgatcctc gcgctgagcg cccggccgct cgaatacgac cccgacggac
     1801 cggccggacg gctggcggaa gtgctgctcg atgccatcgc cgagctgccc gaactcgatt
     1861 tcggcctgcc gctgccggcc gatgcgcgcc tgcgccggct ctgcgaagcc ctgcaggccg
     1921 aaccggccga tgcacgcacg ctgcccgaat gggcggcgcg ctgcggcctg tccgagcgca
     1981 cgctgacgcg gcgcttccgc cgcgagaccg gcctgagctt cgccgactgg cgccggcgcc
     2041 tgcgcctgct gctcgccctc ggcgcgctcg aagccgggcg cagcgtcacc gcggtcgcgc
     2101 tcgacagcgg ctacgattcg ctttccgcct tcatcgccgc cttccgcaac gagttcggca
     2161 cgacgccgaa ggcgctgttc gcgccggcac cggtgaacga tggatgattt gcgactgatg
     2221 atttcccgca tggcgcggca gatgaccctg cccggccatg cctccgcagg gagccgcgca
     2281 tgagcaccga tcctctcctg acctccgact tcgccgaagc cctgctcgcc acgcagaccg
     2341 ggccgcaggc gccggcgacg ctgcgcttcg acgccacccg caccggcctg gccttcggcg
     2401 gcaccgtgcc ggcggtacgc gtctatgcct tcgggccggc cagcctcgcc cgtcactggc
     2461 accccggctt cccgacgccc gcgcagctgg aatacgcgat cgccgccgtc gaggacgaac
     2521 tgatgcgcgt gcaccgccac tgcgggccgc cgccgtcgct ggcgtccgcc gtctgcccgg
     2581 acccggagcc acgggcactg gccgcgagcc tcggcctgcc gggcagcggc cgcgtgcaac
     2641 tgctgcgcga ggccgtcgaa cacggcttcg ggcgcctcgc cgcctgcgcc gaaggccggc
     2701 cgtccgacag cggcggcctg ccgcaggaca cgaacggcat ggcactgctg ctgatcctgc
     2761 gcgaactgat gcaccacctg ccgctcgccg ccctcgaact gccggcctga agacacagaa
     2821 atcgtgggat tgagccggat cacggcgtcg tctagtttct gtccaaatct ttagctttgc
     2881 ggacggactc ccttgatgat cacgcgcatc cggcaaggcc ggacccgtcc cgggcaggcc
     2941 ggcgagtacg aacctctgct gctcgaacac gtgtttccgg agatcgccgc ccgcgccctg
     3001 tcaggctggc ccggctacga gctgctgcgc cgcgagcgca gccatgaggt cgagttcgtg
     3061 accatcatgc atttcgacga tcaggccgca ctccacacct tcgatcacct cgatggatca
     3121 gccgagggcg tgccgcccga agcccggacg ctgctcgcgc acttcgattc cctgtcctcg
     3181 cactaccgga ccgtcgcggt ccgtgccgga ggtcacacca catgaacgcc gtcgccaacc
     3241 gtctgtacct gcgcctcgcc cggccgggcg atgccgatgc gatcgccggc ctgatgacgc
     3301 tgctcggcta tccggtcgaa gccggcgaga ccgccgggcg gctcgaacgg ctgctgcgtc
     3361 atcccgatgc cgcggtgctg gtcggcggcc tcgaggacgg cccggtgcag ggctgtctgg
     3421 cgctgcactt cattccgcag ctcggcctgg ccggcgactt ctgccgcatc gcctacttca
     3481 gcgtcgcccc ggccgcgcag ggacaggggc tcggccgggc gctggaggcc gaggccacgc
     3541 gcctcgcaca gcagcgcggc tgcgaccgca tcgaggtgca cagccacgag cgccgccac
//
LOCUS       DAOQJH010000236         3596 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_16376_length_3596_cov_4.900028, whole genome shotgun sequence.
ACCESSION   DAOQJH010000236 DAOQJH010000000
VERSION     DAOQJH010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3596)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3596)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3596
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16376_length_3596_cov_4.900028"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..434)
                     /locus_tag="PLQ18_06505"
     CDS             complement(<1..434)
                     /locus_tag="PLQ18_06505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE01341.1"
                     /translation="MPQVWEDAWLARSIEAGLPRQAELALLLAARSYHDPVKAEALLQ
                     EAEAAAPGHLAPLVGLYKFYFYQGRLAQALETAERTLLKVARDLGLPEDWRAVERGSR
                     DFSDYADGPHRMYLFVLKAYGYLLARLGELAGSRAVFEKLRLL"
     gene            complement(635..982)
                     /locus_tag="PLQ18_06510"
     CDS             complement(635..982)
                     /locus_tag="PLQ18_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014029045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HNE01342.1"
                     /translation="MSAPTMNLKVSRKAEKFIRRMITFSGKAGGFRLAVKEGGCSGMS
                     YDFSIEAEPAATDAVVTTETGLQVFVPAECQAMLDGIVIECEDSLMNTGLTFFNPNAA
                     KSCGCGTSFTPAA"
     gene            complement(993..1187)
                     /locus_tag="PLQ18_06515"
     CDS             complement(993..1187)
                     /locus_tag="PLQ18_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011501485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNE01343.1"
                     /translation="MAYKIIASQCTACSACEPVCPNAAISQKKGVFVINPVDCTECEG
                     QYDEPQCAAVCPVDNTCVPR"
     gene            complement(1363..2862)
                     /gene="nifB"
                     /locus_tag="PLQ18_06520"
     CDS             complement(1363..2862)
                     /gene="nifB"
                     /locus_tag="PLQ18_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016919567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase cofactor biosynthesis protein NifB"
                     /protein_id="HNE01344.1"
                     /translation="MKQTLADHQGCGTSGGSGKSSCGSSAGPADMAPEIWEKVKNHPC
                     YSEEAHHHYARMHVAVAPACNIQCNYCNRKYDCANESRPGVVSEKLTPEQAARKVLAV
                     ASTIPQMTVLGIAGPGDSLANPAKTFETFERISKVAPDIKLCLSTNGLALPELVPMIK
                     NYNVDHVTITINMVDPEVGEQIYPWIFYKNKRWKGRDAARILTERQLEGLELLTSAGI
                     LAKINSVMIPGINDQHLIEVNKAVKSRGAFLHNIMPLISEAEHGTYFGLNGQRGPTAQ
                     ELKALQDACEGDMNMMRHCRQCRADAVGLLGEDRSAEFTLEKLMEKTEIEYDLEARKA
                     YQELVEAERQDKVAAREKEMVEMASVESDTKILIAVATKGGGRINEHLGHAKEFQIYG
                     VSTAGAKFVGHRRVDLYCQGGYGEEEGLDTIIRAINDCTAVFVAKIGGCPKNDLQAAG
                     IDPVDAYAFEYIEQSALAYFKDYVARVESGELVHTARGDAQIRQGAFAA"
     gene            complement(3310..>3596)
                     /locus_tag="PLQ18_06525"
     CDS             complement(3310..>3596)
                     /locus_tag="PLQ18_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003457190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HNE01345.1"
                     /translation="RRLGFWQAEAPAPERLASSVPFCHDTLEFHEWLQWVFLPRLEAM
                     LASAHLPPFACHIAPLAELSWEHDGREVAHAVALLARIDDLLGDPAQRAH"
BASE COUNT          587 a         1128 c         1208 g          673 t
ORIGIN      
        1 agcaggcgca gtttttcgaa caccgcccgg ctgccggcga gttcgccgag gcgtgcgagc
       61 aggtagccgt aggccttcag cacgaacaga tacatgcggt gcgggccgtc ggcgtaatcg
      121 gagaaatcgc ggctgccgcg ctcgacggcg cgccagtcct cgggcaggcc gaggtcgcgg
      181 gcgaccttca gcagcgtgcg ttcggcggtt tcgagcgcct gcgccagccg cccctggtag
      241 aagtagaact tgtacagccc gaccagcggc gccagatgcc cgggcgcggc ggcctcggcc
      301 tcctgcagca aggcttcggc cttcaccgga tcgtggtagc tgcgggcggc gagcagcagc
      361 gccagttcgg cctgccgcgg caggccggcc tcgatgctgc gggcgagcca ggcgtcttcc
      421 cagacctgcg gcatggcggt gctccggtcg atggtgtgcg gcgcggggcg cggattggtc
      481 agggccagat tccggcgccg gccgggacgc tgcgtccggc tgccgccgcc cgtggcgggc
      541 ggcggcagcg gcagcggccg ggacggtgcg gacagggtga tttgctggat catgaccgcc
      601 gccccctcat ctctctctcg tgcggtatcc ggactcaggc agccggcgtg aacgaggtgc
      661 cgcagccgca gctcttcgcg gcgttggggt tgaagaaggt caggccggtg ttcatcagcg
      721 agtcctcgca ctcgatgacg atgccatcga gcatggcctg gcactcggcc ggcacgaaca
      781 cctgcagacc ggtctcggtc gtgaccacgg cgtcggtggc cgcgggttcg gcctcgatgc
      841 tgaagtcata cgacatcccc gaacagccgc cttccttgac ggcgagccgg aagccaccgg
      901 ccttgccgct gaaggtgatc atgcggcgga tgaatttctc cgccttgcgg ctgaccttga
      961 ggttcatcgt cggcgcgctc atgcgggcag gctcagcgcg gcacgcaggt gttgtcgacc
     1021 gggcagacgg ccgcgcactg cggctcgtcg tactggcctt cgcactcggt gcaatcgacc
     1081 gggttgatca cgaacacgcc cttcttctgg gagatggcgg cattcgggca gaccggctcg
     1141 caggccgagc aggcggtgca ctgggaagcg atgatcttgt aggccatgac ggcagctcct
     1201 ttacggtttg cgaaacgaaa agcgtggaaa cgctgggtga aagccggtac tgccggtcac
     1261 acgggactgc ctggtatttc tccctttctt tccggcccct cccccgcaag tggcgagggg
     1321 ctcagaaagc gcagcgtccc gctgtgaccg gcctgacatg catcaggccg cgaaggcacc
     1381 ctgacggatt tgcgcatcac cgcgcgcggt gtgcacgagt tcgccactct cgacacgggc
     1441 gacgtaatcc ttgaagtacg ccagcgccga ctgctcgatg tattcgaagg cgtaggcatc
     1501 gaccggatcg atgccggcgg cctgcagatc gttcttcggg cagccgccga tcttggcgac
     1561 gaacaccgcg gtgcagtcgt tgatcgcgcg gatgatcgtg tccagacctt cttcctcgcc
     1621 gtagccgccc tggcagtaca gatcgacgcg gcggtgaccg acgaacttcg caccggcggt
     1681 cgagaccccg taaatctgga attccttggc gtgacccaag tgctcgttga tgcggccgcc
     1741 gcccttggtc gccacggcga tcaggatctt ggtgtcggat tcgacgctgg ccatttcgac
     1801 catttccttc tcgcgcgccg caaccttgtc ctgacgctcg gcctcgacca gttcctgata
     1861 ggccttgcgg gcttcgaggt cgtattcgat ctcggtcttt tccatcagct tttcgagcgt
     1921 gaattcagcc gagcgatcct cgccgagcag gccgacggcg tcggcgcggc actggcggca
     1981 gtggcgcatc atgttcatgt cgccttcgca ggcatcctgc agcgccttca gctcctgcgc
     2041 ggtcgggccg cgctggccgt tgaggccgaa gtaggtgccg tgctcggctt ccgagatcag
     2101 cggcatgatg ttgtgcagga acgcgccgcg cgacttgacc gccttgttga cttcgatcag
     2161 gtgctggtcg ttgatgcccg ggatcatcac cgaattgatc ttggcgagaa tgccggccga
     2221 ggtcagcagc tccagacctt cgagctggcg ctcggtcagg atcctggcgg catcgcggcc
     2281 cttccagcgc ttgttcttgt agaagatcca cggatagatc tgctcgccga cttccggatc
     2341 gaccatgttg atggtgatcg tgacgtgatc gacgttgtaa ttcttgatca tcggcaccag
     2401 ctcgggcagc gccagaccat tggtggaaag gcagagcttg atgtccggcg ccaccttcga
     2461 gatgcgctcg aaggtttcga aggtcttggc cggattggcc agcgaatcac cggggccggc
     2521 aatgccgagc acggtcatct gcggaatcgt cgaggccacc gccagcacct tgcgcgcggc
     2581 ctgctcgggc gtcagctttt cactgaccac accggggcgc gattcattgg cgcagtcgta
     2641 cttgcgattg cagtaattgc actggatgtt gcacgccgga gccactgcca cgtgcatgcg
     2701 ggcgtaatgg tgatgcgcct cttcgctgta gcacgggtgg ttcttgacct tttcccagat
     2761 ttccggggcc atgtcggccg gaccggccga ggaaccgcaa ctcgacttgc cgctgccacc
     2821 gctggtacca cagccctgat gatcggcgag cgtctgcttc atctgctcgc ggacttcggg
     2881 gcgaatggtc tgcaggctgg agagggagat gatctgtgac acggcggcac tccttcttgt
     2941 ctgtataacc cgaggcaggg cttgtgacag ggaggagcaa gcgcaatgcc acatccattt
     3001 agctattgaa aatcatgacc ttgcagggat tgtcgcgtgt atcgaaaccg acatccccgt
     3061 tgtggtcatg tcggagaacc cacgtcggac ggcttgtgtc ggcagcgcct ggaaggcgca
     3121 ggcaaggcgt gcgggtcagc aaaccgcagg gtgtgcagct tgctgcgcag cgtgtacgcc
     3181 ggatgcagat tcagggctgc atggtatgca acaaagctgc acaccatgca ggctccggtc
     3241 tcccctctcc ccttgcggga gaggggtggg gggagagggg gaaaacagcg catttacgtg
     3301 cgatcgatct cagtgcgccc gctgcgccgg atcgccgagc agatcgtcga tgcgcgccag
     3361 caacgcgacc gcatgcgcga cctcgcgccc gtcgtgctcc cagctcagct cggcgagcgg
     3421 cgcgatatga cacgcgaacg gcggcaggtg cgcactggcg agcatcgctt cgaggcgcgg
     3481 caggaacacc cactgcagcc actcgtggaa ttcgagcgtg tcgtgacaga acggcacgct
     3541 gctcgcgagc cgctcggggg ccggcgcttc ggcctgccag aagccgaggc gacgca
//
LOCUS       DAOQJH010000237         3588 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_16441_length_3588_cov_3.617888, whole genome shotgun sequence.
ACCESSION   DAOQJH010000237 DAOQJH010000000
VERSION     DAOQJH010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3588
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16441_length_3588_cov_3.617888"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..830)
                     /locus_tag="PLQ18_06530"
     CDS             complement(<1..830)
                     /locus_tag="PLQ18_06530"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNE01346.1"
                     /translation="MPDSAILAGTGERRGAAVPMDPPAAMRSVRPDPELAALAARLSP
                     VHLRQRLNMEGAHGKAVFGYGRTFFHLENWYSIHALIRGVLKLGGLYGRGLRNALAIA
                     VTRHEARLPALPAAFDGLRVLQLSDLHLDASEAFVHALAERVRGVEADLTVLTGDFRY
                     RTHGDWRPALERLERLRPQLPGEVYAILGNHDTVRMVPRIESLGIRLLMNEHVTLTRA
                     DARLHLAGIDDPHFFEAGNIERAADGIPAEDCAILLSHSPETWWQAAHAGFDLLLAGH
                     T"
     gene            complement(1025..2812)
                     /locus_tag="PLQ18_06535"
     CDS             complement(1025..2812)
                     /locus_tag="PLQ18_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018410399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HNE01347.1"
                     /translation="MKASTAPRPQAGRRLGLEEVLRLLVEDGEISEASAAELRKQRTR
                     SANSAHPLVFIGEQRLPSAQQPKRVLSTEMLTEWLAGRTGFPYFHIDPLKIDVSRVCG
                     VVSHAYATRFHILPVAVSDTAVTIATAEPFDREWQSVLEQGLRLNIRPVIANPADIVR
                     YLAEFYSLARSVQSAMKSPQSLPSAITNFEQLVKLGGKHGVDANDRAIVHIVDWLWQY
                     AFEQRASDIHIEPRRDECLVRFRIDGVLHLVYQGPMTVMVAAINRIKLLARMDVVEKR
                     RPQDGRIKTGTPNGEEIELRLSSMPTAFGEKLVLRIFDPNVLLKSRAELGFSDEENRL
                     WSGLVHRPHGIVLVTGPTGSGKTTTLYSTLRELATPEVNVCTIEDPIEMVYPYLNQMQ
                     VQPNLGIDFALGVRTLMRQDPDVIMVGEIRDLETAEMAVQAALTGHLVLSTLHTNDAP
                     AAVVRLMDLGVPPYLIRSTVLGVVAQRLVRTVCPHCAEPEPVDAAAWEALVRPWSVAM
                     PAQSMRGAGCLECRMTGFRGRTGLYEMLMLSPELALHVDSPLDLDAMRAQAIREGMRP
                     LRIAGALKVAAGLTTIDEVARNTPPVGRG"
     gene            complement(3050..>3588)
                     /locus_tag="PLQ18_06540"
     CDS             complement(3050..>3588)
                     /locus_tag="PLQ18_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017482513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutaredoxin"
                     /protein_id="HNE01348.1"
                     /translation="LRRFFGKPLRDPDAVTYQPVIAVFGMAALMALAACQAAFGTVLT
                     RQAGEWFIAFSMCLLAMLKLRDLESFSNMFLGYDLLAQRRVRYAYLYPFGEALAGVLM
                     IAGGAGLWLAVPVAFVIGTIGAISVFKAVYIDRRELRCACVGGDSHVPLGFVSLTEDL
                     MMVAMAVWMGLKYLWPLL"
BASE COUNT          566 a         1213 c         1277 g          532 t
ORIGIN      
        1 gtgtggccgg cgagcagcag gtcgaagccg gcgtgcgcgg cctgccacca ggtttccggc
       61 gagtgcgaca gcaggatcgc gcagtcctcg gccgggatgc cgtcggcggc gcgctcgatg
      121 ttgccggcct cgaagaagtg cggatcatcg atgccggcga ggtgcaggcg cgcgtcggcg
      181 cgcgtcagcg tcacgtgctc gttcatcagc agccggatgc cgagcgactc gatgcgcggc
      241 accatgcgca cggtgtcgtg gttgccgagg atcgcgtaga cctcgcccgg cagctgcgga
      301 cgcaggcgtt cgagccgttc gagcgccgga cgccagtcgc cgtgcgtgcg gtagcggaaa
      361 tcgccggtga gcacggtcag gtcggcctcg acgccgcgca cgcgctcggc cagcgcatgc
      421 acgaaggcct cggaggcatc gaggtgcagg tcggacagct gcagcacgcg caggccgtcg
      481 aaggccgccg gcagcgccgg cagccgcgcc tcgtgccggg tcacggcgat cgcgagcgcg
      541 ttgcgcagcc cccggccgta gaggccgccg agcttcagca cgccgcggat cagcgcgtgg
      601 atcgaatacc agttttcgag atggaagaag gtgcggccgt agccgaacac ggccttgccg
      661 tgcgcgccct ccatgttcag gcgctggcgc agatgcaccg ggctcaggcg ggccgcgagc
      721 gcggcgagtt cgggatcggg ccggacggag cgcatggcag caggggggtc catcggtacg
      781 gcagcgccgc gccgttcgcc ggtgccggcg agaatcgcgg aatcaggcat gttcggtcac
      841 tccccggttt gttgcccctt ccctggagag aggctttgca gtgcgcgcgg ccccgcacgc
      901 gtgacgatcg cccgcattta agcagccgcc accggcatgc ggcggcgcac caatgatgaa
      961 aagttgataa gggccttgta tcgcagcgat ccgttgccag cgcgcaacag cgccgccggc
     1021 gccctcagcc cctgccgacc ggcggcgtgt tgcgggcgac ctcgtcgatc gtcgtcaggc
     1081 cggcggcgac cttcagcgcg ccggcgatgc gcagcggccg catgccctcg cggatcgcct
     1141 gcgcgcgcat cgcgtccaga tcgagcggcg aatcgacgtg cagggccagc tccggactca
     1201 gcatcagcat ctcgtacagg ccggtgcggc cgcggaagcc ggtcatccgg cattcgaggc
     1261 agccggcacc gcgcatggac tgcgccggca tcgccaccga ccacggccgc accagggctt
     1321 cccaggcggc ggcatcgacc ggctcgggtt cggcgcagtg cggacagacg gtgcgcacca
     1381 gccgctgcgc gaccacgccg agcacggtcg agcggatcag gtagggtggc acgccgaggt
     1441 ccatcaggcg cacgaccgcc gcgggcgcat cgttggtgtg cagcgtcgac agcaccagat
     1501 gcccggtcag cgccgcctgc accgccatct cggcggtttc gaggtcgcgg atctcgccga
     1561 ccatgatcac gtccgggtcc tgacgcatca gcgtgcgcac gccgagcgcg aagtcgatgc
     1621 cgagattcgg ctgtacctgc atctgattca gatacgggta caccatctcg atcgggtcct
     1681 cgatcgtgca gacgttgacc tcgggcgtcg ccagctcgcg cagcgtcgag tacagcgtcg
     1741 tggtcttgcc cgagccggtc gggccggtga ccagcacgat gccgtgcggc cggtgcacga
     1801 gtccgctcca gaggcggttc tcctcgtccg agaagccgag ttcggcgcgg ctcttcagca
     1861 gcacgttcgg atcgaagatg cgcagcacca gcttctcgcc gaaggcggtc ggcatcgacg
     1921 acaggcgcag ctcgatctcc tcgccgttcg gcgtgccggt cttgatgcgc ccgtcctgcg
     1981 gccggcgttt ctcgaccacg tccatgcgtg cgagcagctt gatgcggttg atcgccgcga
     2041 ccatcaccgt catcggtccc tgatagacga ggtgcagcac gccgtcgatg cgaaagcgca
     2101 ccaggcattc gtcgcggcgc ggttcgatgt ggatgtcgct ggcgcgctgc tcgaaggcgt
     2161 actgccacag ccagtcgacg atgtggacga tggcgcggtc gttggcatcg acgccgtgct
     2221 tgccgccgag cttgaccagc tgctcgaagt tggtgatcgc gctcggcagc gactgcggcg
     2281 atttcatcgc cgactgcacc gagcgcgcga ggctgtagaa ctcggcgaga tagcggacga
     2341 tgtcggccgg attggcgatc accggccgga tgttcaggcg cagcccctgt tcgagcacgc
     2401 tctgccattc gcggtcgaag ggctcggcgg tggcgatggt gacggcggtg tcgctgactg
     2461 cgaccggcag gatgtggaag cgcgtggcgt aggcgtgcga gacgacgccg cagactctgg
     2521 acacgtcgat cttcagcggg tcgatgtgga agtacggaaa gccggtgcgc ccggcgagcc
     2581 attcggtcag catctcggtg ctgagcacgc gcttcggctg ctgcgcgctc ggcaggcgct
     2641 gctcgccgat gaacaccagc ggatgcgcgc tgttcgcgct gcgcgtgcgc tgcttgcgca
     2701 gttcggccgc actcgcctcg ctgatctcgc cgtcctcgac cagcagccgc agaacctctt
     2761 cgagcccgag cctgcgcccg gcctgcggcc gcggcgccgt ggatgccttc atgatcactg
     2821 cctcgctgtc gctgccggtg ccgcagcggc ggaagcgccg gcaccgtccc cgcaccggcc
     2881 tgcggcgcgg ggtcggacac cttagaggat ttcggccggt ggtggcatgg gtggtccggt
     2941 caagaaatgc gaaaccgccg gacccggacg ggcagcgcgg caggcagccg gagatgcagc
     3001 aggccaaggc gggcggactt gtgcggacag agacccgcgc ccgccggctt tacagcaggg
     3061 gccacagata cttcagcccc atccacaccg ccatcgccac catcatcaga tcctcggtca
     3121 gcgacacgaa gccgagcggc acgtggctgt cgccgccgac gcaggcgcat ctgagttcgc
     3181 gccggtcgat atagacggcc ttgaacaccg agatcgcgcc gatcgtgccg atcacgaacg
     3241 cgaccggcac ggccagccag agtccggcgc cgccggcgat catcagcacg ccggcgagcg
     3301 cctcgccgaa cggatagagg tacgcatacc gcacccggcg ctgcgcgagc aggtcgtagc
     3361 cgaggaacat gttcgagaag ctttcgaggt cgcgcagctt cagcatcgcg agcaggcaca
     3421 tcgagaaggc gatgaaccat tccccggcct gccgcgtgag cacggtgccg aacgcggcct
     3481 ggcaggcggc cagcgccatc agcgccgcca tcccgaacac cgcgatgacc ggctggtagg
     3541 tgacggcgtc cggatcgcgg aggggcttgc cgaagaagcg gcgcaggt
//
LOCUS       DAOQJH010000238         3586 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_16459_length_3586_cov_3.406400, whole genome shotgun sequence.
ACCESSION   DAOQJH010000238 DAOQJH010000000
VERSION     DAOQJH010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3586
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16459_length_3586_cov_3.406400"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            139..1368
                     /locus_tag="PLQ18_06545"
     CDS             139..1368
                     /locus_tag="PLQ18_06545"
                     /inference="COORDINATES: protein motif:HMM:NF012739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNE01349.1"
                     /translation="MIRLFTRPLSRLFRRPDLILMRLVTAWIFTGALIVLVAGCDSRA
                     NNDSANHAMPPPEVARSVVRAADVELPLEYVAQVGGSREVQVRARVEGILQKRTYTEG
                     KPVKAGETLFLIDPAPFQAALDRARADLAEQEARLAQADKELKRVQPLFADRAVSQKD
                     RDAAQAERDQASAAVKSARAAVRTAEIDLGYTRVTAPIAGITSREVRSEGSLVRPGDA
                     SGALTTLNQVDPAYVRFTYAENEALMIRKSIAEGRLRMPAGGPQVEVVLADGSVHPQA
                     GRIDFTDTLVDPQTGTVGVRATVANPDATLLPGQFVRVRLKGVTRPGAIVVPQRAVMQ
                     GPDGQFVWVIGDGDKVEFRPVVTGASIGKGWIVEEGLKGGERVATDNLLKLAPGALVV
                     EPKPAPAAAAGGHAPQG"
     gene            1453..>3586
                     /locus_tag="PLQ18_06550"
     CDS             1453..>3586
                     /locus_tag="PLQ18_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003242824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNE01350.1"
                     /translation="MNSRYFIERPILASVLSIVIVLAGLASMRALPIAQYPDIVPPEV
                     QVTAFYPGASAEVVAATVAAPLEQAINGVDDMLYVRSNSSAGALTIAASFAIGTDPDQ
                     ATINVNNRVQSALQSLPEEVRRQGVKAEKQSSNLLQIVMLDSPSKRFDTVFISNYALV
                     NVVDELRRINGVGNAMIFSARDYSMRIWIRPDRLASLGLTPSDIAAAVREQNSQFAAG
                     KFGQAPMGTPQEFTYTVTTQGRLATPEEFGAIVLRAEADGTLLRLRDVARIELGAADY
                     GMVAKRSGNETVAIGIFLAPGANALAVAGAVDARMKELARHFPVGLQWTAPYDTTRFV
                     EISIREVVKTLAEAMGLVFLVVFLFLQSARATIIPFLAVPVSLIGTFAGMYLLGFSIN
                     TLTLFGMVLAIGIVVDDAIVVLENVERIMRTQRLPPREATIQAMQEVSGPVIAIVLVL
                     CAVFVPVGFAGGLAGQMYKQFAITIAVSVVLSGFVALTLTPALCATLLKPVAHEPGRF
                     FRAFNRGFEHVTNGYSAGVRFFLKRALLGVALFAGMLVLTWGLFQRVPGSLVPDEDQG
                     YVMTMAILPPGASLERTIAVNDRINEFALANPAVDNVIALSGLDLLSFSNVPFYGAAF
                     IPLKHWEDRTAPGQDAQSLVGQFFMQGAQIRDAIVLAFNPPPISGMSTTGGFEAYLQS
                     RGGGGSKALAGEVQKFLAAAGQRPELAGL"
BASE COUNT          523 a         1270 c         1229 g          564 t
ORIGIN      
        1 tgcggacagt gtatcgcttc gcggggcttt gcatacatac atgaatgtat gtaaagtgca
       61 ctgcaacatc atcgaacgtc gcgccgtggc gcttgtccgc gcacacgcat ccgccggtgc
      121 gctccgggag tcctgcccat gatccgcctg ttcacgcgcc cgctgtcacg gctcttccgc
      181 cggccggacc tgatcctgat gcgcctcgtc accgcatgga tcttcaccgg tgccctgatc
      241 gtgctggtcg ccggctgcga cagccgcgcc aacaatgaca gcgctaacca cgcgatgccg
      301 ccgcccgagg tcgcgcgcag tgtcgtccgt gcggccgatg tcgagctgcc gctcgaatac
      361 gtcgcgcagg tcggcggttc gcgcgaggtg caggtgcggg cgcgcgtcga gggcatcctg
      421 cagaagcgca cctacaccga aggcaagccg gtcaaggccg gcgagacgct gttcctgatc
      481 gacccggcgc ctttccaggc cgcgctcgat cgcgcccgtg ccgatctcgc cgagcaggag
      541 gcgcggcttg cgcaggcgga caaggagctg aagcgcgtgc agccgctgtt cgccgaccgg
      601 gccgtcagcc agaaggaccg cgacgccgcg caggccgagc gcgatcaggc gagtgcggcg
      661 gtcaagtccg cgcgcgccgc ggtgcgcacg gccgagatcg acctcggcta cacgcgcgtg
      721 accgccccga tcgccggcat caccagccgc gaggtgcgct ccgaaggcag cctcgtgcgt
      781 cccggcgatg caagcggcgc gctgacgacg ctgaaccagg tcgatccggc ctacgtgcgc
      841 ttcacctacg ccgagaacga ggctctgatg atccgcaagt cgatcgccga gggccggttg
      901 cgcatgccgg ccggcggacc gcaggtcgag gtcgtgctcg ccgatggcag cgtgcatccg
      961 caggccgggc ggatcgactt caccgacacg ctggtcgatc cgcagaccgg tacggtcggc
     1021 gtgcgcgcga cggtcgccaa ccccgatgcc acgctgctgc ccggccagtt cgtgcgcgtg
     1081 cggctgaagg gcgtcacgcg ccccggcgcg atcgtcgtgc cgcagcgtgc ggtcatgcag
     1141 ggaccggacg gccagttcgt ctgggtcatt ggcgacggcg acaaggtcga gttccggccg
     1201 gtcgtcaccg gcgcctcgat cggcaagggc tggatcgtcg aggaagggct gaagggcggc
     1261 gagcgggtcg cgaccgacaa cctgctgaag ctcgcgcccg gcgcgctcgt cgtcgagccg
     1321 aaaccggcac cggccgccgc ggccggcggc cacgcgccgc agggctgaac ccgctctggc
     1381 acccattgca tcgccggcgc gcgcccgccc gcgcgccgtg ccgtcccgcg cccacccccg
     1441 gggagcccgc gcatgaattc ccgctacttc atcgaacgcc cgatcctcgc gagcgtgctg
     1501 tcgatcgtca tcgtgctcgc cggcctcgcg tcgatgcgcg cgctgccgat cgcgcaatac
     1561 ccggacatcg tgccgcccga ggtgcaggtc acggccttct acccgggtgc cagcgccgag
     1621 gtggtcgccg cgaccgtcgc cgcgccgctc gaacaggcga tcaacggcgt cgacgacatg
     1681 ctgtacgtgc gctcgaactc ctcggccggc gcgctgacca tcgccgccag cttcgcgatc
     1741 ggcaccgatc cggatcaggc gacgatcaac gtcaacaacc gcgtgcagtc ggcgctgcag
     1801 tcgctgcccg aggaagtgcg ccgccagggc gtcaaggccg aaaaacagag ctcgaacctg
     1861 ctgcagatcg tcatgctcga ttcgccaagc aagcgcttcg acacggtgtt catcagcaac
     1921 tacgcgctgg tcaacgtcgt cgacgagctg cgccgcatca acggcgtcgg caacgcgatg
     1981 atcttctcgg cgcgcgacta ctcgatgcgc atctggatcc ggcccgaccg gctcgcgagc
     2041 ctcgggctga cgccgtccga catcgccgcc gcggtgcgcg agcagaactc gcagttcgcg
     2101 gccggcaagt tcgggcaggc gccgatgggc acgccgcagg aattcaccta cacggtgacg
     2161 acgcaggggc gtctggcgac gccggaggaa ttcggcgcga tcgtgctgcg cgccgaggcc
     2221 gacggcacgc tgctcaggct ccgtgatgtc gcgcgcatcg agctcggcgc cgccgactac
     2281 ggcatggtcg ccaagcgcag cggcaacgag acggtcgcga tcggcatctt cctcgcgccc
     2341 ggtgccaatg cgctcgcggt cgccggcgcc gtcgatgcgc gcatgaagga gctggcccgg
     2401 cacttcccgg tcggcctgca gtggacggcg ccgtatgaca cgacgcgctt cgtcgagatc
     2461 tccatccgcg aggtggtcaa gaccctcgcc gaggcgatgg gtctggtgtt cctcgtcgtg
     2521 ttcctgttcc tgcagagcgc gcgcgcgacc atcatcccgt tcctggccgt gccggtgtcg
     2581 ctgatcggca ccttcgccgg catgtacctg ctcggcttct cgatcaacac gctgacgctg
     2641 ttcggcatgg tgctggcgat cggcatcgtc gtcgatgacg ccatcgtcgt gctggagaac
     2701 gtcgagcgca tcatgcgcac ccagcgcctg ccgccgcgcg aggcgacgat ccaggcgatg
     2761 caggaggtca gcggcccggt catcgccatc gtgctcgtgc tctgtgcggt gttcgtgccg
     2821 gtcggcttcg ccggcggact ggccgggcag atgtacaagc agttcgcgat cacgatcgcc
     2881 gtgtcggtcg tgctgtcggg cttcgtcgcg ctgacgctga cgccggcgct gtgcgcgacg
     2941 ctgctgaagc ccgtggccca cgagccgggg cggttcttcc gcgccttcaa ccgcggcttc
     3001 gagcacgtca ccaacggcta ttcggccggc gtgcgcttct tcctgaagcg cgcgctgctc
     3061 ggcgtggcgc tgttcgccgg catgctggtg ctgacctggg gactgttcca gcgcgtgccg
     3121 ggctcgctgg tgccggatga ggatcagggc tacgtgatga cgatggcgat cctgccgccc
     3181 ggcgcctcgc tcgaacgcac gatcgccgtc aacgaccgca tcaacgagtt cgcgctcgca
     3241 aaccccgcgg tcgacaacgt catcgcgctg tcggggctgg atctgctgtc gttctcgaac
     3301 gtgccgttct acggcgccgc cttcatcccg ctgaagcact gggaagaccg caccgcgccc
     3361 ggtcaggacg cgcagtcgct ggtcgggcag ttcttcatgc agggcgcgca gatccgcgat
     3421 gccatcgtgc tcgcgttcaa cccgccgcca atcagcggca tgtcgacgac cggcggcttc
     3481 gaggcctatc tgcagagccg cggcggcggc gggtcgaagg cgctggccgg cgaggtgcag
     3541 aagttcctcg ccgccgcggg ccagcgtccg gagctcgccg ggctga
//
LOCUS       DAOQJH010000239         3578 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_16507_length_3578_cov_4.957707, whole genome shotgun sequence.
ACCESSION   DAOQJH010000239 DAOQJH010000000
VERSION     DAOQJH010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3578)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3578)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3578
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16507_length_3578_cov_4.957707"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /locus_tag="PLQ18_06555"
     CDS             complement(<1..285)
                     /locus_tag="PLQ18_06555"
                     /inference="COORDINATES: protein motif:HMM:NF024901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HNE01351.1"
                     /translation="MNRARPALLLVLGWLALGALPADAAVYKWVDAEGRTHYSSTPPP
                     GADAKPVVIREDPLPPPAAGSPPAAPPAAGGGNADNAPRPPDQARAAACDT"
     gene            561..2303
                     /locus_tag="PLQ18_06560"
     CDS             561..2303
                     /locus_tag="PLQ18_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018122290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HNE01352.1"
                     /translation="MELTGAEIVVRCLKEEGVEYLFGYPGGSVLHIYDALHGQDDVKH
                     VLVRHEQAAAHAADGYSRASGKPGVVLVTSGPGLTNAVTGIATAYMDSVPMVILSGQV
                     PTKLIGNDAFQEVDSVGITRPCVKHNFLVKDVKDLAVTIKKAFYIATTGRPGPVLVDL
                     PKDITAARVEFDYPKKIKLRSYNPTVKGHAGQIRKAVELLVQARRPMIYTGGGVVLSD
                     GSKDLVELTRLLNFPITNTLMGLGAYPATDRQFVGMLGMHGTYEANLAMHHCDVLFAV
                     GARFDDRVTGNLEKFCPNARIIHIDIDPSSISKNVPVELPIVGSVPNVLRQMIAVLKE
                     EGLKPDAEAIAQWWRQIDEWRAIESLAYRNSDTIIKPQYVVEQLYKVTGGQAIVTSDV
                     GQHQMWAAQYYLFDRPRQWINSGGLGTMGFGLPAAMGAKFAFPDLDVACITGEGSIQM
                     MLQELSTMKQYGTPVKIVNLNNRYLGMVRQWQEFFYEKRYSMSYMEALPDFRKLAEAY
                     GHIGLQIEKPGDVEGALREAFAMKDRTVFLDFLTDPEENVYPMIPAGGGQAEMLLAGR
                     DAMKNVHEEGMILV"
     gene            2306..2797
                     /gene="ilvN"
                     /locus_tag="PLQ18_06565"
     CDS             2306..2797
                     /gene="ilvN"
                     /locus_tag="PLQ18_06565"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020564705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HNE01353.1"
                     /translation="MRHIISILLENESGALSRVAGLFSARGYNIESLTVAPTEDESLS
                     RLTLVTYGNDAIIEQIVKQLNKLIDVVKLVDLTEGAHIERELMLVKVRATGELRAEMF
                     RLTEIFRGRMLDVSESTYTIEMTGPSDKLDAFLGTVGADNVVEVARSGVTGIARGPKG
                     LHV"
     gene            2876..>3578
                     /gene="ilvC"
                     /locus_tag="PLQ18_06570"
     CDS             2876..>3578
                     /gene="ilvC"
                     /locus_tag="PLQ18_06570"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011352711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HNE01354.1"
                     /translation="MNVYYDKDADLSLIQGKTVAIIGYGSQGHAHANNLSESGVQVVV
                     GLRAGGASWQKAVNAGLSVKEVDAAVEGADVVMILLPDENIAGVYQQIEKKIKQGAAL
                     AFAHGFNIHYNQVVPRADLDVIMIAPKAPGHTVRSTYKAGGGVPHLIAVYQDKSGAAR
                     DIAMSYAVANGGGKAGIIETSFREETETDLFGEQAVLCGGVSALVTAGFETLVNAGYQ
                     PEIAYFECLHELKLIV"
BASE COUNT          621 a         1196 c         1193 g          568 t
ORIGIN      
        1 ggtgtcgcag gcagcggcgc gcgcctgatc gggcgggcgc ggcgcgttgt cggcattgcc
       61 cccgcccgcg gccggcggtg cggccggcgg cgaaccggcg gcgggcggcg gcagcgggtc
      121 ctcgcggatg acgaccggct tcgcatcggc cccgggcggg ggcgtggacg aataatgcgt
      181 gcggccttcg gcatcgaccc acttgtagac cgcggcatcg gcgggcaggg cgccgagtgc
      241 gagccagccg agaaccagca gcagggccgg gcgggcgcgg ttcatgatgc gtactcctgc
      301 ggtttcagcg tgttgggagg gcggtcgtgc ggcgggaatg cccgccgtcc ttgacctttg
      361 ggggggctgc ggatagtttg cccggtccgt gcgaactggc tgccaccgat gaccccgtcc
      421 gcgactccgc gggcggtgcc cgaaaggccg ttcctgctga tccgcggggc gcgcagccac
      481 gacgataccg gcgattttgc cagtctgcgc ggcgccacgc cgccgtcaac ccatatgccc
      541 agtcccagga ggctcctgcc gtggaactca ccggtgccga gatcgttgtc cgttgcctga
      601 aggaagaggg ggtcgaatac ctcttcggct atccgggcgg ctccgtactg cacatctacg
      661 atgcgctgca cggccaggac gacgtgaagc acgtgctggt gcggcatgaa caggccgccg
      721 cgcacgccgc cgatggttac tcgcgtgcct cgggcaagcc cggcgtcgtg ctcgtgacct
      781 ccggtccggg cctgaccaac gccgtcaccg gcatcgccac cgcctacatg gattcggtgc
      841 cgatggtgat cctgtccggg caggtgccga ccaagctgat cggcaacgat gccttccagg
      901 aggtcgactc ggtcggcatc acgcgtccgt gcgtgaagca caacttcctg gtcaaggacg
      961 tgaaggatct cgcggtcacg atcaagaagg ctttctacat cgccacgacc ggccgccccg
     1021 gtccggtgct ggtcgacctg ccgaaggaca tcaccgccgc gcgcgtcgag ttcgactacc
     1081 cgaagaagat caagctgcgc tcgtacaacc cgaccgtgaa gggccacgcc ggccagatcc
     1141 gcaaggcggt cgagctgctg gtgcaggcgc gccggccgat gatctacacc ggcggcggcg
     1201 tcgtgctgtc ggacggttcg aaggatctcg tcgagctcac ccggctgctg aacttcccga
     1261 tcaccaacac gctgatgggg ctcggtgcct atccggccac cgaccgccag ttcgtcggca
     1321 tgctcggcat gcacggcacc tatgaagcga atctggcgat gcatcactgc gacgtgctgt
     1381 tcgccgtcgg tgcgcgcttc gatgaccgcg tgaccggcaa cctcgaaaag ttctgcccga
     1441 acgcccgcat catccacatc gacatcgatc cgtcgtcgat ctcgaagaac gtgccggtcg
     1501 agctgccgat cgtcggctcg gtgccgaacg tgctgcgcca gatgatcgcg gtgctgaagg
     1561 aggaaggcct caagcccgat gccgaggcca tcgcccagtg gtggcggcag atcgacgaat
     1621 ggcgcgcgat cgagtcgctc gcgtaccgca acagcgacac gatcatcaag ccgcagtacg
     1681 tggtcgagca gctctacaag gtcaccggcg gccaggcgat cgtgacctcc gacgtcggcc
     1741 agcaccagat gtgggctgcg cagtactacc tgttcgaccg tccgcgccag tggatcaact
     1801 ccggcggcct cggcacgatg ggcttcggcc tgccggcggc gatgggcgcg aagttcgcgt
     1861 tcccggacct cgacgtggcc tgcatcaccg gcgagggcag catccagatg atgctgcagg
     1921 aactctcgac gatgaagcag tacggcacgc cggtgaagat cgtgaacctg aacaaccgct
     1981 atctgggcat ggtgcgccag tggcaggagt tcttctacga gaagcgctac tcgatgtcgt
     2041 acatggaagc gctgccggac ttccgcaagc tcgccgaggc ctacggccac atcggcctgc
     2101 agatcgagaa gccgggcgat gtcgaaggcg cactgcgcga ggccttcgcg atgaaggacc
     2161 gcaccgtgtt cctcgacttc ctgaccgatc ccgaagagaa cgtctacccg atgatccctg
     2221 ccggcggcgg ccaggccgag atgctgctcg ccgggcgcga cgcgatgaag aacgtgcacg
     2281 aagaagggat gatcctggtg taatcatgcg acacatcatt tcgatcctgc tggagaacga
     2341 atccggcgcc ctgtcgcgcg tggcgggcct gttttccgcc cgcggctaca acatcgagtc
     2401 gctgaccgtc gcgccgaccg aggatgagtc gctgtcgcgg ctgacgctgg tcacctacgg
     2461 caacgacgcg atcatcgagc agatcgtcaa gcagctgaac aagctgatcg acgtggtcaa
     2521 gctcgtcgac ctgaccgaag gggcgcacat cgagcgcgag ctgatgctcg tgaaggtgcg
     2581 cgcgaccggc gagctgcgcg ccgagatgtt ccggctcacc gagatcttcc gcggccgcat
     2641 gctcgacgtg tccgagagca cgtacacgat cgagatgacc ggcccgagcg acaagctcga
     2701 tgccttcctc ggcaccgtcg gtgccgacaa cgtcgtcgag gtggcgcgtt ccggcgtcac
     2761 cggcatcgcg cgcgggccca agggcctgca cgtctgaacg actgactcca ccttcctggc
     2821 ggggccggcc gctaccaagg cctgccccgc gctaccgccc gcagggaact cccgcatgaa
     2881 cgtttactac gacaaagacg ccgacctctc cctcatccag ggcaagaccg ttgccatcat
     2941 cggctacggc tcgcagggtc acgcccacgc caacaacctc agcgagtccg gcgtgcaggt
     3001 cgtcgtcggc ctgcgtgccg gcggtgcctc gtggcagaag gccgtcaacg ccggcctgag
     3061 cgtcaaggaa gtcgacgccg ccgtcgaggg cgccgacgtg gtcatgatcc tgctgccgga
     3121 cgagaacatc gccggcgtct atcagcagat cgagaagaag atcaagcagg gtgcggcgct
     3181 cgccttcgcg cacggcttca acatccatta caaccaggtc gtgccgcgcg ccgatctcga
     3241 cgtgatcatg atcgcgccga aggccccggg ccacaccgtg cgctcgacct acaaggccgg
     3301 cggcggcgtg ccgcacctga tcgccgtcta tcaggacaag tccggcgcgg cccgcgacat
     3361 cgcgatgtcc tacgcggtcg ccaacggcgg cggcaaggcc ggcatcatcg agaccagctt
     3421 ccgcgaggag accgagaccg acctgttcgg cgagcaggcc gtgctgtgtg gcggcgtgtc
     3481 cgcgttggtc acggcgggct tcgagactct ggtgaacgcg ggctaccagc ccgagatcgc
     3541 ctacttcgag tgcctgcacg agctgaagct catcgtcg
//
LOCUS       DAOQJH010000240         3577 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_16515_length_3577_cov_4.184554, whole genome shotgun sequence.
ACCESSION   DAOQJH010000240 DAOQJH010000000
VERSION     DAOQJH010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3577)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3577)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3577
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16515_length_3577_cov_4.184554"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..350
                     /locus_tag="PLQ18_06575"
     CDS             <1..350
                     /locus_tag="PLQ18_06575"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HNE01355.1"
                     /translation="VPAKPPARPRTPQPAAEPLARAPDTAWVVQLAATESETRLRSYA
                     ERNGLSGYVILRTRRNGQDWHVLLLGGFHPNQAAAETALTRLPEALRSETTPWVRSVR
                     SLRRLLPPADPAP"
     gene            608..>3577
                     /gene="gltB"
                     /locus_tag="PLQ18_06580"
     CDS             608..>3577
                     /gene="gltB"
                     /locus_tag="PLQ18_06580"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019583678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HNE01356.1"
                     /translation="MQTNQQQGLYRPEFERDNCGFGLIAQMDDQASHWLVKTSIAALA
                     RLTHRGAVAADGKSGDGCGLLMKKPDGFLRIVAAEAGLTLGSLYACGLVFLNTDAQKA
                     QAARTLLETELGKEGLTVAGWRTVPTDPACCGEEAMKTLPLFEQIFINAPEGMDPAQF
                     ERHLVVARRRTAYGLMDSDKAFYVVTLSAHVLGYKGLVMPAALPEFYKDLEDPRLESS
                     ICVFHQRFSTNTMPQWKLAQPFRYLAHNGEINTVQGNRNWALARGYTFSSPLLPMQDL
                     RPMVNMTGSDSSSLDNMLEVLLAGGMDVFRAMRLLVPPAWQNVDHLDPDLRAFYEYNS
                     MHMEPWDGPAGIVLTDGRYAACTLDRNGLRPARWVITKDRHMTIASEIGVYDYTPGEV
                     VAKGRVKPGEMLAVDTATGTLLSPADIDNLLKTRKPYRQWLAQNTKYLKSIFPKDVAS
                     SEPLKADELAVYEKLFQVSFEERDQVLRILAEGAQEAVGSMGDDTPFAVLSERARSPY
                     DYFRQQFAQVTNPPIDPLREAIVMSLEGCMGAEGNMFVEDELLSERLVISSPVLSEGK
                     FRAILKLNDGRYAHQRIDLNYPASEGLAQAVARFADEAVAAVQAGKVMIVLSDRSIEP
                     GRLPAHAALAVGAVHQRLVHEGLRCKANLMVETATARDPHQFAVLIGFGASMVYPYLA
                     FEVIEDMVRTGEITSKDTAKLAENYRKGINKGLFKIISKMGISTINSYRGAQLFEIVG
                     LHEEVVGLCFTGTTSRIQGANFADLEADVQALAAGAWRPRVAREQGGLLKYVHGGEYH
                     AYNPDVVQQLQAAVNSGDFKLWKVYSESVNTRPVATFRDLLQLKADGAKPVPLDQVEP
                     LDAILKRFDSAGMSLGALSPEAHETLAEAMNRLGGRSNSGEGGEDPARYGTVKVSKIK
                     QIASGRFGVTPAYLTSAEVLQIKVAQGAKPGEGGQLPGDKVDATIARLRFSRPGVALI
                     SPPPHHDIYSIEDLAQLI"
BASE COUNT          598 a         1288 c         1168 g          523 t
ORIGIN      
        1 ccgtgcccgc aaaaccgccg gcacgtccgc gcacgccgca gccggcggcg gagccgctcg
       61 cgcgcgcacc ggatacggcc tgggtggtgc agctcgccgc gaccgaatcc gaaacgcggc
      121 tgcgcagcta cgccgaacgc aacggcctca gcggctacgt gatcctgcgc acccggcgca
      181 acggccagga ctggcacgtg ctgctgctcg gcgggttcca tccgaatcag gcggcggccg
      241 aaacggccct gacccgcctg cccgaggcgc tgcgcagcga aaccacgccc tgggtgcgca
      301 gcgtgcgttc gctgcgccgg ctgctgccgc ccgccgatcc cgcgccctga cggcgcgggc
      361 tgcaccgttt cggcgcaccc gcatcgggca tgcgcgattg agtcaggctg cgggaaaagt
      421 caaggcttcg ctgtccgctt acgtttaccg atgcggcact gcaccgttat actgcgcggt
      481 ccgcgtcact ggccgccggc atgaatcctg ctcataccgg ctccgatgcg cagccagccc
      541 cccacggtcc ggcatccccg gccggccgtc acccacgtta gcggcatact ctgcaggcgg
      601 agcaaagatg cagacgaatc agcagcaggg cctgtaccgg cccgaattcg agcgcgacaa
      661 ctgcggcttc ggcctcatcg cacagatgga cgatcaggcc agccactggc tcgtcaagac
      721 ctccatcgcc gcgctcgccc gcctgaccca ccgcggcgcg gttgccgccg atggcaagtc
      781 cggtgacggc tgcggcctgc tgatgaagaa acccgacggc ttcctgcgca tcgtcgccgc
      841 cgaggccggc ctgacgctgg gctcgctgta tgcctgcggc ctggtgttcc tgaacaccga
      901 tgcgcagaag gcgcaggccg cgcgcacgct gctcgaaacc gaactcggca aggaaggcct
      961 gacggtcgcc ggctggcgca ccgtgccgac cgatcccgcc tgctgcggcg aggaagccat
     1021 gaagacgctg ccgctgttcg agcagatctt catcaacgcc cccgaaggca tggacccggc
     1081 gcagttcgag cgccacctgg tcgtcgcccg ccggcgcacg gcctacgggc tgatggacag
     1141 cgacaaggcc ttctacgtcg tgacgctgtc ggcgcatgtg ctcggctaca agggtctggt
     1201 gatgccggcg gcgctgccgg agttctacaa ggatctcgaa gacccgcggc tggaatcgtc
     1261 gatctgcgtg ttccaccagc gcttctcgac caacacgatg ccgcagtgga agctcgcgca
     1321 gccgttccgc tacctcgcgc acaacggcga gatcaacacg gtccagggca accgcaactg
     1381 ggcgctggcg cgcggctaca cgttctcgtc gccgctgctg ccgatgcagg acctgcggcc
     1441 gatggtcaac atgaccggct cggactccag ctcgctcgac aacatgctcg aagtgctgct
     1501 cgccggcggc atggacgtgt tccgtgcgat gcgcctgctg gtgccgccgg cctggcagaa
     1561 cgtcgatcac ctcgatccgg acctgcgcgc gttctacgag tacaactcca tgcacatgga
     1621 gccgtgggac ggcccggccg gcatcgtgct gaccgacggc cgctacgccg cgtgcacgct
     1681 cgatcgcaac ggcctgcggc cggcgcgctg ggtgatcacg aaggaccggc acatgacgat
     1741 cgcctcggag atcggcgtct acgactacac gcccggggag gtcgtcgcca agggccgcgt
     1801 caagcccggc gagatgctgg ccgtcgacac cgcgaccggc acgctgctca gccccgccga
     1861 catcgacaac ctgctgaaga cgcgcaagcc ctaccgccag tggctcgcgc agaacaccaa
     1921 gtacctgaag tcgatcttcc cgaaggatgt cgccagctcc gagccgctga aggccgacga
     1981 gctcgccgtc tacgaaaagc tcttccaggt cagcttcgag gagcgcgacc aggtgctgcg
     2041 catcctcgcc gagggcgcgc aggaagccgt cggctcgatg ggcgacgaca cgccgttcgc
     2101 ggtgctctcc gagcgcgcgc gttcgccgta cgactacttc cgccagcagt tcgcgcaggt
     2161 cacgaacccg ccgatcgatc cgctgcgcga agcgatcgtc atgtcgctcg aaggctgcat
     2221 gggcgccgaa ggcaacatgt tcgtcgagga cgagctgctg tccgaacgcc tcgtgatctc
     2281 ctcgccggtg ctctccgaag gcaagttccg cgcgatcctg aagctcaacg acggccgcta
     2341 cgcgcaccag cgcatcgacc tgaactaccc ggcctccgaa ggtctggcgc aggcggtcgc
     2401 gcgcttcgcc gacgaggcgg tcgccgccgt gcaggccggc aaggtcatga tcgtgctgtc
     2461 cgaccgctcg atcgagccgg gccggctgcc ggcgcacgcc gcgctcgccg tcggtgccgt
     2521 gcaccagcgc ctcgtgcacg agggcctgcg ctgcaaggcc aacctgatgg tcgagaccgc
     2581 gaccgcccgc gacccgcacc agttcgcggt gctgatcggc ttcggcgcgt cgatggtcta
     2641 cccctacctg gccttcgagg tcatcgagga tatggtccgc accggcgaga tcacctcgaa
     2701 ggacacggcc aagctcgccg agaactaccg caagggcatc aacaagggcc tgttcaagat
     2761 catctcgaag atgggcatca gcaccatcaa cagctaccgc ggcgcgcagc tgttcgagat
     2821 cgtcggcctg cacgaggaag tcgtcgggct ctgcttcacc ggcaccacca gccgcatcca
     2881 gggcgcgaac ttcgccgacc tcgaagccga cgtgcaggcg ctcgccgccg gtgcctggcg
     2941 cccgcgcgtg gcgcgcgagc agggcggact gctgaagtac gtgcacggcg gcgagtacca
     3001 cgcctacaac ccggacgtgg tccagcagct gcaggccgcc gtcaacagcg gcgacttcaa
     3061 gctctggaag gtttattccg agtccgtcaa cacccggccg gtcgcgacct tccgcgacct
     3121 gctgcagctg aaggccgatg gtgcgaagcc ggtgccgctc gatcaggtcg agccgctcga
     3181 tgcgatcctc aagcgcttcg actcggccgg catgtcgctc ggcgcgctgt cgcccgaggc
     3241 gcacgagacg ctcgccgaag ccatgaaccg cctcggcggg cgctcgaact ccggcgaggg
     3301 cggcgaggac ccggcgcgct acggcacggt gaaggtctcg aagatcaagc agatcgcctc
     3361 ggggcgcttc ggcgtgacgc cggcgtatct caccagcgcc gaggtgctgc agatcaaggt
     3421 cgcgcagggc gccaagcccg gcgagggcgg ccagctgccc ggtgacaagg tcgatgcgac
     3481 gattgcccgg ctgcgcttct cgcgcccggg tgtcgcgctg atctcgccgc cgccgcacca
     3541 cgacatctac tcgatcgagg acctcgcgca gctgatc
//
LOCUS       DAOQJH010000241         3569 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_16569_length_3569_cov_2.635458, whole genome shotgun sequence.
ACCESSION   DAOQJH010000241 DAOQJH010000000
VERSION     DAOQJH010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3569)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3569)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3569
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16569_length_3569_cov_2.635458"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1280
                     /locus_tag="PLQ18_06585"
     CDS             <1..1280
                     /locus_tag="PLQ18_06585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="chromosome segregation ATPase-like protein"
                     /protein_id="HNE01357.1"
                     /translation="AELAALAPARQPEDAQGDALADLEQPPACLVGASSPGAQPLLPK
                     PQRDEGEAAALERVQAHLSALGLRFPERTLHAFHTALKTATISPLTVLAGISGTGKSQ
                     LPRRYAEAMGIHFLKLPVQPRWDSPQDMLGFYNYLEKRYKATEFARALVHFDAHNWPH
                     ARPYRDRLLLVLLDEMNLARVEYYFSEFLSQLEGRPAPKDHDPERIRNSEIVLDAGGA
                     GGVPPRIYPGHNLLFVGTMNEDESTQTLSDKVLDRANLLRFPRPDRLVGEALSGDGRP
                     ASGYLPESRWHGWRRSFGQLPKAVQQPVERWIQHLNEDLDGLHRPFAHRVNQAMLAYV
                     ANYPGVAEPGAGDALACARLAFADQLEQRILPKLRGVDLGDAGVASHLERIGRLIEVD
                     LDDPKLATAFRRAREDDGSGRPFLWTGVRRESA"
     gene            1277..2956
                     /locus_tag="PLQ18_06590"
     CDS             1277..2956
                     /locus_tag="PLQ18_06590"
                     /inference="COORDINATES: protein motif:HMM:NF021352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2357 domain-containing protein"
                     /protein_id="HNE01358.1"
                     /translation="MIPFVLSLPWGLFAQTPLDGRPVRHALRPGDAVELDGRHGTALA
                     VAAGTGLKLSGRRWSTQPLGDAFPWDGAPMQLHALPGAALSSERRVRIDLHRSGQPPL
                     DCELLLQAALLRPRGGAADTPAALAMLEFLHQTLGRLREVEAADADADAGWREDPTAW
                     DRLQRAWPERQAQAEEARHALIVEHAGRLPALVEATAGHPRRVLKRTRQQMPIDRIQE
                     LDTACLEWMIRRPGLSVAEKAGPRQRLLGVAREENLDTLENRVLKDFLRLSIEEAGLW
                     QHANQRFGLSERARRVGRYQALCRNCHRELRAAGISDPVAPVLPNFVLQHDARYRVIW
                     RAYRELLSAEQREDDLWRWQGRLWADFVRLAVVIGLGRIADPQVRLQAPLRLRREQAR
                     GRWSDTLARLAVFLLTLRGRPLVAEVLDPGLPQPAAQPAGLAAVAPWQHALGCTAVIV
                     LRDGRDGRQRSLCVWAAHGVTDAPLSLETLAASADEALDQAMRREALHGDDRIRARGL
                     VIRSKHDAAPEFATQAGRVYGLTLSADPARIREAFAEFALTLQDSLERLFA"
     gene            2953..>3569
                     /locus_tag="PLQ18_06595"
     CDS             2953..>3569
                     /locus_tag="PLQ18_06595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE01359.1"
                     /translation="MSLSPGIDLNGVCDCTVLPDGALRLAAAPPVVIDASPLGVLTGR
                     MALDAPAGRSGWPAEDGIRLPVLSLLRALGHDDDAPLDAHEVDAPEAARLLGRHLAGL
                     LPARVRTAVAAVPDTDGFDERTRARLLDGAARAGIDLSLLWRPVAALLGWGEALDARA
                     LQALHGRSACVMQLLADGIALGSYGLEAVEQGGRLTLVPVRRRDGL"
BASE COUNT          490 a         1343 c         1245 g          491 t
ORIGIN      
        1 gcgccgagct tgccgcgctt gcccccgccc ggcagccgga ggatgcgcag ggcgacgcgc
       61 tcgccgacct cgaacagccc ccggcctgcc tggtcggtgc cagcagcccg ggcgcacaac
      121 cgttgctgcc aaagccgcag cgcgacgagg gcgaggcggc cgcgctcgaa cgggtgcagg
      181 cgcatctgtc cgcgctcggc ctgcgcttcc ccgaacgcac gctgcatgcg ttccacaccg
      241 cgctcaagac cgcgacgatc agcccgctga ccgtgctcgc cggcatctcc ggcaccggca
      301 agagccagct gccgcgccgc tacgccgagg cgatgggcat ccacttcctg aagctgccgg
      361 tgcagccgcg ctgggacagc ccgcaggaca tgctcggctt ctacaactat ctggagaaac
      421 gctacaaggc gaccgaattc gcgcgggccc tcgtgcattt cgacgcccac aactggccgc
      481 acgcccggcc gtaccgcgac cgcctgctgc tcgtgctgct cgatgaaatg aacctcgcac
      541 gcgtcgagta ctacttcagc gagttcctga gccagctcga aggccggccg gctccgaagg
      601 accacgatcc cgaacgcatc cgcaattccg aaatcgtgct cgatgccggc ggggccggcg
      661 gcgtgccgcc gcgcatctac cccggccaca acctgctgtt cgtcggcacc atgaacgagg
      721 acgaatcgac gcagacgctc tcggacaagg tgctcgatcg cgccaacctg ctgcgcttcc
      781 cgcgccccga ccggctggtc ggcgaggccc tgagcggtga cggccggccg gccagtggct
      841 atctgcctga atcccgctgg catggctggc gccggtcgtt cggccagttg ccgaaagccg
      901 tgcagcagcc ggttgagcgc tggatccagc acctcaacga ggacctcgac ggcctgcacc
      961 gtccgttcgc gcaccgggtc aaccaggcca tgctcgccta cgtcgccaac tacccgggcg
     1021 tcgccgaacc cggcgccggc gatgcgctgg cctgtgcgcg cctcgccttc gccgaccagc
     1081 tcgaacagcg catcctgccc aagctgcgcg gcgtcgatct cggcgatgcc ggcgtcgcct
     1141 cgcacctgga acgcatcggc cggctgatcg aggtcgatct ggacgacccg aagctggcca
     1201 ccgccttccg gcgcgcccgc gaggatgacg gcagcggccg gccgttcctg tggacgggcg
     1261 tgcgccgcga gtcggcatga tcccgttcgt gctgtcgctg ccctggggac tgttcgcgca
     1321 gaccccgctc gacggccggc ccgtgcgcca tgccctgcgc cccggggacg ccgtggagct
     1381 cgatggccgt cacggcacgg cgctggcggt agcggccggt accgggctga agctgagcgg
     1441 ccggcgctgg agcacgcagc ccctggggga tgcattcccg tgggacggcg ctccgatgca
     1501 gctgcatgcc ctgccgggcg cggcgctgag cagcgagcgt cgggtgcgca tcgacctgca
     1561 ccgcagcggc cagccgccgc tcgactgcga actgctgctg caggcagcgc tgctgcgccc
     1621 gcgcggcggc gcagccgaca ccccggctgc cctcgccatg ctggaattcc tgcaccagac
     1681 cctcggacgc ctgcgcgagg tcgaggccgc cgatgccgat gccgatgccg gctggcgcga
     1741 agacccgacc gcctgggacc ggctgcagcg cgcctggccc gagcgccagg cgcaagccga
     1801 agaagcgcgc cacgcgctga tcgtcgagca cgccggacgc ctgccggcgc tggtcgaagc
     1861 cacggccgga catccgcggc gcgtgctgaa gcgcacgcgc cagcagatgc cgatcgaccg
     1921 catccaggaa ctcgataccg cctgtctgga atggatgatc cgcaggcccg gcctgtccgt
     1981 ggccgagaag gccggcccgc gccagcgtct gctcggcgtc gcccgcgagg aaaacctcga
     2041 cacgctggaa aatcgcgtgc tgaaggactt cctgcgcctg agcatcgagg aagccggact
     2101 ctggcagcac gccaaccagc gcttcggcct cagcgagcgc gcgcggcggg tcggccgcta
     2161 tcaggcgctg tgccgcaact gccaccgcga gctgcgcgcc gccggcatca gcgaccccgt
     2221 cgccccggtg ctgccgaatt tcgtgctgca gcacgacgcc cgctatcgcg tgatctggcg
     2281 ggcctatcgc gaactgctga gcgccgagca gcgcgaggat gatctctggc gctggcaggg
     2341 acggctctgg gccgacttcg tgcggctcgc ggtcgtcatc ggactcggac gcatcgccga
     2401 tccgcaggta cgcctgcagg caccgctgcg gctgcggcgc gagcaggccc gcggccgctg
     2461 gtccgacacg ctcgcccggc tggccgtgtt cctgctcacg ctgcggggca ggccgctggt
     2521 ggccgaagtg ctcgatcccg gcctgccgca gcccgccgcg cagcccgccg gccttgccgc
     2581 cgtggcgccg tggcagcatg cgctcggctg caccgccgtc atcgtgctgc gtgacggccg
     2641 cgatggccgc cagcgcagcc tctgcgtgtg ggcggcgcac ggcgtcaccg atgcccccct
     2701 gagcctggaa acgctggcag cctccgccga cgaagccctg gatcaggcga tgcgccggga
     2761 ggccctgcat ggcgacgacc ggatccgcgc ccgcgggctg gtgatccgct cgaaacacga
     2821 tgcggcaccg gaattcgcca cccaggccgg ccgggtctat ggcctgacgc tgtcggccga
     2881 tccggcgcgc atccgcgagg cgtttgccga gttcgcgctg accctgcagg acagcctgga
     2941 gaggctgttc gcatgagcct ctcgcccggc atcgatctca acggcgtctg tgactgcacc
     3001 gtgctgccgg acggtgcgct gcggctggca gccgcaccgc cggtggtgat cgacgcgtcc
     3061 ccgctcggcg tcctgaccgg ccgcatggcg ctggacgcgc cggccgggcg cagcggctgg
     3121 ccggccgaag acggcatccg cctgccggtg ctgagcctgc tgcgggcgct cgggcacgac
     3181 gacgatgcgc ctctcgacgc ccacgaagtc gacgcccccg aagcggcccg tctgctcggc
     3241 cggcatctgg ccggcctgct gcccgcgcgg gtccgcaccg ccgtggcagc ggtgccggac
     3301 accgacgggt ttgatgagcg cacccgcgcc cggctgctgg acggagcggc acgcgccggc
     3361 atcgacctga gcctgctgtg gcgcccggtc gcggccttgc tcggctgggg cgaagcgctg
     3421 gatgcccggg cgctgcaggc cttgcatggt cgcagtgcct gcgtcatgca gctgctggcc
     3481 gatggcatcg ccctgggcag ctacggcctc gaagccgtcg aacagggcgg tcgtctgacg
     3541 ctggtcccgg tgcgccggcg cgatggcct
//
LOCUS       DAOQJH010000242         3566 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_16590_length_3566_cov_3.937055, whole genome shotgun sequence.
ACCESSION   DAOQJH010000242 DAOQJH010000000
VERSION     DAOQJH010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3566)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3566)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3566
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16590_length_3566_cov_3.937055"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1104)
                     /locus_tag="PLQ18_06600"
     CDS             complement(<1..1104)
                     /locus_tag="PLQ18_06600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE01360.1"
                     /translation="MSALAARERLAPVLGASAAIVAHYLRFEAAWLRAWFGDELPPLI
                     CLHALSRRLHPRLPSHSLAALAGRLGSEPAQRHRATDHVAATRAIWRAMLAELADHGI
                     RTLPALLAWLSEPSGPASTGAREYGLDRRLRLALPDAPGVYRYLDARGQVLYVGKATS
                     LHKRVNSYFRKRRGDSAAIRELLTRATGIETTVCASAVHAAILECREIQHLDPPFNTA
                     LKQSRRPLYWLDHELGMVATGHDQRYRIGPWSGSWNVELWLGACEAVFDGDLRRLQGI
                     TAARSDETPQPDAVRTVLLAAWQHPPDRRSLLARAIRRHRHDRSTETGPAAEESEGHA
                     SEQGRRPEASAGATEHRLETFDPVTCSARALATI"
     gene            complement(1323..1913)
                     /locus_tag="PLQ18_06605"
     CDS             complement(1323..1913)
                     /locus_tag="PLQ18_06605"
                     /inference="COORDINATES: protein motif:HMM:NF025244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4202 domain-containing protein"
                     /protein_id="HNE01361.1"
                     /translation="MSRTADALAAFDAINAEDPQRIEVAGVPTPRTLVHARSVSQWIE
                     RLHPQASEALLLAARAHHLRRWEHPRSQWPMTREGYLRWRLELKRIHAEVAAGVLGAL
                     GYEPELISRVGALIRRERLKLDPEAQALEDAICLAFLEHDFDHFGPQHDPAKVIAIVR
                     KTWAKMSEHGRSAALTLSLSDYAQSIVAQALAPTQA"
     gene            complement(2025..2948)
                     /locus_tag="PLQ18_06610"
     CDS             complement(2025..2948)
                     /locus_tag="PLQ18_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006999614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HNE01362.1"
                     /translation="MSPRDRKAELARRLEENHQRHSSARQELGFDAGRRHLMLPVDAI
                     DPNPFQPRKVFDEDELRQLAGSIAELGQHQPVVVRPAGERWQLVAGERRWRACRLLGK
                     PTIEALVQDADDARMAVTALAENMEREDLSDYEIGIAIRRIEDQFPSKAKLAEALGRT
                     REDLYKYLAFEALPEDLRARLDAQPRLLSRAAAADVKRMLADERALPLLREAWTKLEA
                     GELQQTRLAAFVAAGLGKAEPAPQRKAVERALHRFTREGQHVGTVSRDAAHFVIRLKV
                     DALSGAQEQRLRSFVEALLAESGPDRNESGT"
     gene            complement(2945..>3566)
                     /locus_tag="PLQ18_06615"
     CDS             complement(2945..>3566)
                     /locus_tag="PLQ18_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNE01363.1"
                     /translation="TQNLLLAEPEKLPLAIHDDTNIEGVALIYGSLALGRTEDQLRER
                     TPRPAEELRSKLAPLDGIYDVVLMDCPPSLKLLTSNALAAATHLIIPVISGSQYGMYG
                     VTDLMHHVGMIRRINPGLALLGALLMRHDERQTVCKLIEGTARAEIGRLLPVRISTST
                     KVEQAAMANASLHRWDRSAKVSREFHQLAVALAAELQLSVSGEEDE"
BASE COUNT          511 a         1176 c         1285 g          594 t
ORIGIN      
        1 gatcgtggcc agtgcccggg cactgcaggt gactggatcg aaggtctcca ggcggtgttc
       61 ggtcgctccg gccgatgcct ctggtctgcg gccttgctct gatgcatgcc cttcgctttc
      121 ttccgcagcc ggaccggttt cggtggaccg atcgtgacga tggcgtcgga tggcgcgcgc
      181 gagcaggctg cgccggtctg gcggatgctg ccacgccgcc agcagcacgg ttcgcacggc
      241 gtccggttgc ggggtttcat cgctccgcgc agcggtgatc ccttgcagcc tgcgaaggtc
      301 gccgtcgaac acggcctcgc aggcgccaag ccagagttcg acattccagc tcccgctcca
      361 cggaccgatc cggtatcgct ggtcgtgccc ggtcgcgacc atacccagct cgtgatcgag
      421 ccagtacagc ggacgccggg attgcttcag cgcggtgttg aacggcgggt cgagatgctg
      481 gatttcgcgg cattcgagga tcgcggcatg cacggcgctc gcacagaccg tggtttcgat
      541 cccggtcgca cgggtcagca gttcgcggat tgcggcgcta tccccccggc gcttgcggaa
      601 atagctgttt acgcgcttgt gcagggatgt ggccttgccg acgtacagca cctgcccgcg
      661 ggcatcgaga tagcgataga cccccggggc atccggcagg gccagtcgca gccggcgatc
      721 gaggccgtat tcgcgtgctc cggtgctggc tggtccgctc ggttcgctca gccaggccag
      781 cagcgccggc agggtccgga tgccgtggtc tgcgagttcg gcaagcatgg cgcgccagat
      841 cgcccgcgtg gcggcaacgt gatcggtggc gcgatggcgc tgtgccggtt cgctgcccag
      901 gcggcctgcc agggcagcga gcgaatggct gggcaggcgc ggatgcaggc gtctggacag
      961 ggcgtgcaga cagatcagcg gcggcagctc gtcaccgaac caggcacgca gccaggccgc
     1021 ttcgaagcgc aggtaatgcg cgacgatcgc agccgaggct cccagcaccg gcgccagccg
     1081 ttcgcgggcg gcgagcgctg acaaagcccc cgccgtgccg ggttcacagc cgcgccggcg
     1141 caggatcgcg ggcaggcgcg ccagggctgg cagcgcgatc gggcaggact cgatggtctc
     1201 tccggccgaa gtctgcagcc accaggcgag atcgaaggga tgatcgacgt ccgggcgggg
     1261 accggtcgcc tgcagatcga gcagcagcag cggcagggtt tcaagccgct gttcctgcgt
     1321 gctcatgcct gcgttggtgc cagcgcctgc gcgacgatcg attgcgcgta gtccgacagg
     1381 gacagcgtca gcgcagccga gcggccgtgt tcggacatct tcgcccaggt cttgcgcacg
     1441 atggcgatga ccttggccgg atcgtgctgc ggaccgaagt gatcgaagtc gtgttcgagg
     1501 aaggccaggc agatggcgtc ctcgagcgcc tgcgcttccg ggtcgagctt cagccgctcg
     1561 cgccggatca gggcgccgac ccggctgatc agctcgggct cgtagccaag cgcgcccagc
     1621 acgccggcgg cgacctcggc atgaatgcgc ttcagctcca ggcgccagcg cagatagcct
     1681 tcgcgcgtca tcggccattg cgaacgcggg tgttcccagc gacgcaggtg atgggcgcgc
     1741 gctgcgagca gcagggcttc gctcgcctgc ggatgcaggc gttcgatcca ttgcgaaacg
     1801 ctgcgggcgt ggaccagcgt gcgcggggtc ggcacgccgg ctacttcgat gcgctgcgga
     1861 tcctcggcgt tgatggcgtc gaaggcagcg agagcgtcgg cggttcggga catggcggcg
     1921 gttcgggtca ggaaggggga aacgcagcgt aacccgctgc aggctcaccc gtccgccctg
     1981 tgcaggccgc ggtgtagcaa cttgctacac cgcgcaggcc gggttcaggt gcctgattcg
     2041 ttgcggtccg ggccggattc cgccagcagg gcttcgacga agcttcgcag tcgctgttcc
     2101 tgcgcgccgc tgagggcgtc gaccttcagg cggatcacga agtgtgcggc gtcccgcgag
     2161 acggtgccga cgtgctgccc ttcgcgcgtg aagcgatgca gcgcgcgttc gacagccttg
     2221 cgctgcgggg cgggttcagc cttgccgaga ccggcggcga caaaggcggc gagccgggtc
     2281 tgctgcagct cgccggcttc gagcttcgtc caggcctcgc gcagcagcgg cagggcgcgt
     2341 tcatcggcca gcatgcgttt cacgtcggcc gccgccgccc gcgacagcag ccggggctgt
     2401 gcatcgagcc gggcccgcag atcctcgggc agcgcctcga aggcaagata cttgtagagg
     2461 tcctcgcggg tgcgacccag ggcttcggca agcttggcct tggagggaaa ctgatcctcg
     2521 atgcggcgga tggcgatgcc gatctcgtaa tcgctgagat cctcgcgctc catgttctcg
     2581 gccagcgccg tgaccgccat gcgcgcatcg tcggcgtcct gcaccagcgc ctcgatggtg
     2641 ggcttgccga gcagccggca ggcccgccag cggcgttcac cggcgaccag ctgccagcgc
     2701 tcgcccgccg ggcgcacgac gaccggctgg tgctggccga gttcggcgat cgagccggcg
     2761 agctggcgaa gctcgtcttc gtcgaacacc ttgcgcggct ggaacggatt cggatcgatc
     2821 gcatcgaccg gcagcatcag gtggcggcga ccggcgtcga aaccgagctc ctgacgggcg
     2881 ctgctgtgac gctgatggtt ctcttcgagc cggcgggcca gctcggcctt gcggtcacgc
     2941 gggctcattc gtcctcctcc ccgctcacgc tcagctgcag ttcggcggcc agcgccacgg
     3001 ccagctgatg gaattcgcgc gagaccttcg cgctccggtc ccagcggtgc aggctggcat
     3061 tggccatcgc cgcctgctcg accttggtgc tggtcgagat gcgcaccggc agcagccggc
     3121 cgatctcggc cctggccgta ccctcgatca gcttgcagac ggtctggcgt tcgtcgtgac
     3181 gcatcagcag cgcaccgagc agcgccagcc cggggttgat gcgccggatc atgccgacgt
     3241 gatgcatcag gtcggtgacg ccgtacatgc cgtactgcga gccggagatc accgggatga
     3301 tcagatgcgt agcggcggcc agcgcgttgc tggtcagcag cttcaggctg ggcgggcagt
     3361 ccatcagcac cacgtcgtag atgccgtcga gcggcgcgag cttgctgcgc agttcctcgg
     3421 ccgggcgggg cgtgcgttcg cgcagctggt cctcggtgcg tccgagcgcc agcgagccgt
     3481 agatcagcgc cacgccctcg atgttggtgt cgtcatggat cgccagcggc agtttttccg
     3541 gctcggcgag cagcaggttc tgcgtc
//
LOCUS       DAOQJH010000243         3561 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_16631_length_3561_cov_5.165145, whole genome shotgun sequence.
ACCESSION   DAOQJH010000243 DAOQJH010000000
VERSION     DAOQJH010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3561)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3561)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3561
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16631_length_3561_cov_5.165145"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..619)
                     /locus_tag="PLQ18_06620"
     CDS             complement(<1..619)
                     /locus_tag="PLQ18_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004315760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HNE01364.1"
                     /translation="MNAVVQTEQSSFSDFHVADLSLAGWGRKEIAIAETEMPGLMAVR
                     AEYATSQPLKGARITGSLHMTIQTAVLIETLVALGAEVRWASCNIFSTQDHAAAAIAA
                     AGIPVFAYKGESLDDYWDYSHRIFEWADGGYSNMILDDGGDATLLLMLGTRAESDRSV
                     LAKPTCEEEIALFASIERTLARDPKWYSTRLACIKGVTEETTTGVH"
     regulatory      complement(693..769)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01057"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="S-adenosyl-L-homocysteine riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosyl-L-homocysteine"
                     /db_xref="RFAM:RF01057"
     gene            1291..2037
                     /locus_tag="PLQ18_06625"
     CDS             1291..2037
                     /locus_tag="PLQ18_06625"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HNE01365.1"
                     /translation="MIDPGFDLFATDAAGTFATLPGIGPVQLEGLPFDTGAGDRGNVD
                     TILLRRGPALTLNGIGDSGSMPLELVALSLRSVAPVQISPGVFADVHVNVNKPIEDIG
                     APGGNDDGACDPGESCTAANPDRPLDALPASAGALTITRTGENGGTFDSFFDVFAEIT
                     LTLPGTDTVIGSSTFQDTFVQQSGLWSSTPGVGDAHTAQLPAGGFLPGVVAELALLAS
                     HRAGPAIVPEPATLALFGLGALWLSRRRPG"
     gene            2110..2886
                     /locus_tag="PLQ18_06630"
     CDS             2110..2886
                     /locus_tag="PLQ18_06630"
                     /inference="COORDINATES: protein motif:HMM:NF019225.2"
                     /note="PEP-CTERM proteins occur, often in large numbers,
                     in the proteomes of bacteria that also encode an
                     exosortase, a predicted intramembrane cysteine proteinase.
                     The presence of a PEP-CTERM domain at a protein's
                     C-terminus predicts cleavage within the sorting domain,
                     followed by covalent anchoring to some some component of
                     the (usually Gram-negative) cell surface. Many PEP-CTERM
                     proteins exhibit an unusual sequence composition that
                     includes large numbers of potential glycosylation sites.
                     Expression of one such protein has been shown restore the
                     ability of a bacterium to form floc, a type of biofilm;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HNE01366.1"
                     /translation="MNRLSIWLCRLAGLPLLWLTLAVPAHAVPLANGLDGFRTTPGSS
                     FTLPAALPAGFFGSKNGTDSELIAAGTVIAVEGNIALPFATAKKFLVGPGCHTGSTNH
                     HCYEMPVIELFDTVVRRTTDAELTPGGGPVAIGVMLEYLSLRSSSPLEVHYGSEPSSF
                     FDIFVELDGPQTAGSISIHDFQSDNTGLMDTVLPVTYSVTFAEVGGGLQTTINGLQDV
                     MQSQNNSIFVVPEPGTLWLLGAAAVAGLRRRRRIPDRTRA"
     gene            2978..>3561
                     /locus_tag="PLQ18_06635"
     CDS             2978..>3561
                     /locus_tag="PLQ18_06635"
                     /inference="COORDINATES: protein motif:HMM:NF019624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1702 family protein"
                     /protein_id="HNE01367.1"
                     /translation="MARSAGPACVLLGIGREAASFERRGFLPAPPAVQQRLEGIGRTF
                     IDGYRAALSAASAEALAAQLDAEIPLAGRGFAYEGAGMGLALLDLLMPWTASRLQAFL
                     AGPALAHCYIVHVGAGWALARLPWGEGYRRRLDPLLGWLAYDGWGFHRGYFGGGHIPA
                     AYPARLGMAARRVHDQGLGRSLWFVCGADPWRVS"
BASE COUNT          499 a         1229 c         1221 g          612 t
ORIGIN      
        1 ggtgcacgcc ggtggtggtc tcctcggtga cgcccttgat gcaggcgagc cgcgtcgaat
       61 accacttcgg atcacgcgcc agcgtgcgct cgatcgacgc gaacagcgcg atctcctcct
      121 cgcaggtcgg cttcgccagc accgagcggt cggactcggc gcgcgtgccg agcatcagca
      181 gcagcgtcgc gtcaccgccg tcgtcgagga tcatgttcga gtagccgccg tcggcccact
      241 cgaagatgcg gtgcgagtag tcccagtaat cgtccagcga ttcgcccttg tacgcgaaca
      301 ccggaatgcc ggcggcggcg atcgcggcgg cggcatggtc ctgggtcgag aagatgttgc
      361 acgaggccca gcgcacctcg gcaccgagcg cgaccagcgt ctcgatcagc acggcggtct
      421 ggatcgtcat gtgcagcgag ccggtgatgc gcgcgccctt gagcggctgg ctcgtcgcgt
      481 actcggcgcg cacggccatc aggccgggca tctcggtttc ggcgatcgcg atttccttgc
      541 gtccccagcc ggcgagcgac aggtcggcga cgtggaaatc ggagaaggag gactgttcgg
      601 tctggaccac agcgttcatg gagagggctc ctgtcgatgt ggccggggga gatgtcgtcg
      661 gctcacccgg gacatcccgt gcccggcacg gggtcaggtg agcgccgttg ggaaacggtg
      721 accgagcctg gcgggtgggt gtttccccgt tgcagcgctc ctcgggcagg gcgcgaatca
      781 tacggagcac ggccgttgtc gccaagccgg cacgtgggtg caggcccgcg tccggacgcg
      841 tgtatttcaa tttctttaca gggttttgcc gcttcgggca gcgcaaccgt cgccgggtcc
      901 atgggcggtg ggccgtccgg cccctgactg tgctgcctgt ggctgccgta cacagccata
      961 cagacgcgtg atgtccggtt tttttacatc catgaaacca gtgtcgcagt gcgtttgttc
     1021 gatgcagttg atctgattga tttttatcgc tgtggcgtag ccaatgcatt gtcaggactg
     1081 accaccggga aacaaccggg gcattccggg ctgccgttca cggccgcagt ccggcctacc
     1141 acagaggtga tacgtcgatc tgtccgtgtc agtttgatcg ggcaggccgg ggggagaaga
     1201 acgatgtcaa tgcagacaag gttggggccg cccgcgctgg ccctgacggg cgcgttttgg
     1261 ctggccgctc cggcgatggc ggtgccgctg gtgatcgacc ccgggttcga cctgttcgca
     1321 accgatgccg ccggcacgtt cgcgaccctg cccggcatcg ggccggtgca gctcgaaggt
     1381 ctgcccttcg ataccggggc cggtgaccgt ggcaacgtcg acaccatcct gctgcgccgg
     1441 gggccggcgc tgacgctcaa cggcatcggc gactcgggca gcatgccgct cgaactcgtc
     1501 gcactgtcgc tgcgcagcgt tgcgccggtg cagatcagcc ccggcgtgtt cgccgatgtc
     1561 catgtgaacg tcaacaagcc gatcgaggac atcggcgctc ccggtggcaa cgacgacggt
     1621 gcctgcgatc ccggcgagtc ctgcacggcc gcgaaccccg atcgcccgct cgatgcgctg
     1681 ccggcctcgg ccggtgcact gacgatcacg cgcaccggcg agaacggcgg caccttcgat
     1741 tcgttcttcg acgtgttcgc cgagatcacg ctgacgctgc ccggcacgga caccgtgatc
     1801 ggcagcagca ccttccagga caccttcgtg cagcagtccg gcctctggag cagcacgccg
     1861 ggtgtcggcg atgcccatac ggcccagctc cccgccgggg gtttcctgcc gggtgtggtc
     1921 gccgaactgg ccctgctcgc cagtcaccgg gcgggtccgg ccatcgtccc ggagccggcg
     1981 accctggcgc tgttcggact cggtgccctc tggctgagcc ggcgccgacc gggctgaagc
     2041 accgctgcgg tgccctctcc gggggcttga tccagcgcgc cctgcgctgc cgacatcggg
     2101 agaagcgcaa tgaacaggtt gtcgatctgg ctgtgtcgac tggccggtct gccgctgctg
     2161 tggctgaccc tggcggttcc cgcccacgcc gtcccgctcg cgaacgggct ggacgggttc
     2221 aggaccaccc cggggtcctc gttcacgctg ccggcggcgc tgccggccgg ctttttcggc
     2281 agcaagaacg gcaccgattc ggagctgatc gctgccggta ctgtgatcgc cgtcgaaggc
     2341 aacatcgcgc tgccgttcgc aaccgcgaag aagttcctgg tcggccccgg ctgccatacc
     2401 ggctcgacga accaccactg ctacgagatg ccggtcatcg agctgttcga cacggtcgtg
     2461 cgccgcacca ccgatgccga actgaccccc ggcggcgggc cggtcgccat cggcgtgatg
     2521 ctcgaatacc tgtcgctgag atcgagcagc ccgctcgaag tccactacgg gtccgaaccg
     2581 tcgagcttct tcgacatctt cgtcgaactc gacggcccgc agaccgccgg cagcatcagc
     2641 atccacgact tccagagcga caacaccggc ctgatggata ccgtgctgcc ggtgacctac
     2701 agcgtgacct tcgccgaggt gggcggtggc ctgcagacca cgatcaacgg cctgcaggac
     2761 gtgatgcagt cgcagaacaa cagcatcttc gtcgtgccgg agccgggaac gctctggctg
     2821 ctcggcgccg cggccgtcgc gggcctgcgc cgtcgccgcc ggatacccga ccgcacccgg
     2881 gcctgaacgg cgccgtgtga tcccctccct gcgccgcgtc ccgatgcggg ccggcgcacc
     2941 ggcttccccg aagcctttcc cttttccgga ggccgccatg gcccgatccg ccggacctgc
     3001 ctgcgtgctg ctcggcatcg gccgcgaagc ggcgagcttc gagcggcgcg gtttcctgcc
     3061 ggcgcctccg gccgtgcagc agcggctcga aggcatcggc cggaccttca tcgacggata
     3121 ccgggccgcc ctgtccgctg cctcggccga agcgctcgcc gcacaactcg atgcggaaat
     3181 cccgctggcc gggcgcggct ttgcctacga gggcgccggc atggggctcg cgctgctcga
     3241 tctgctgatg ccgtggacgg cgtcgcggct gcaggccttc ctcgccgggc cggcgcttgc
     3301 gcattgctac atcgtccacg tcggcgccgg ctgggcgctg gcgcggctgc cctggggcga
     3361 gggctaccgg cggcggctcg atccgctgct cggctggctg gcctacgacg gctggggctt
     3421 tcaccgcggc tacttcggcg gcgggcacat tccggcggcg tatccggcgc ggctcgggat
     3481 ggcagcccgc cgggtccacg accaggggct cggccgcagc ctgtggttcg tctgcggggc
     3541 tgacccctgg cgcgtgtcca t
//
LOCUS       DAOQJH010000244         3553 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_16683_length_3553_cov_3.941395, whole genome shotgun sequence.
ACCESSION   DAOQJH010000244 DAOQJH010000000
VERSION     DAOQJH010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3553)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3553)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3553
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16683_length_3553_cov_3.941395"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1269
                     /locus_tag="PLQ18_06640"
     CDS             <1..1269
                     /locus_tag="PLQ18_06640"
                     /inference="COORDINATES: protein motif:HMM:NF024985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HNE01368.1"
                     /translation="VRRVTLPTGDSRLAITGVSTQIQPETALLASDRPLLVLSQHYRN
                     DALSGDTLLERFVGREIEAVRVNPASGEEQREKAVLLSVREGQAVFRIGERIESDGAL
                     SPWRLVVERGQDVREEPTLLADLNSSVGGEHLIELSYLSRGFSWDADYVLRLNPGNDG
                     MDLLAWATLHNASGTTFTNARLSLVSGEVNRIAGMRGEAAADLRSAPDLPGGMPGGAA
                     GDDRLFEYQLYTIERPVTLPARQSVHVPLFSTSRAPVRKLYRTTAPLALFGAVIGNQD
                     LRIATALSFANVSPGLGRPLPSGAVRVYQREPSGTGQFLGEARINPVAEGDDAIIEVG
                     ATLDVSARRRQIDFKRLPRDEQEAAWVIDFRNTKTEAVTVDVTQPFGGQWRIVEESLP
                     HRRDAADTASWSVPVPAGGRVTLSYRARSR"
     gene            1325..1684
                     /locus_tag="PLQ18_06645"
     CDS             1325..1684
                     /locus_tag="PLQ18_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HNE01369.1"
                     /translation="MERELCALTDIPDGGTRGIRLQPGARYADLVLARRGAQVYAYRN
                     SCPHTGAPMERESGDDDFLDYQGLHIQCGIHAAIFRFEDGFCLWGPCARRRLEAVAVR
                     IDDGRVIALDVRPWLRD"
     gene            1736..2203
                     /locus_tag="PLQ18_06650"
     CDS             1736..2203
                     /locus_tag="PLQ18_06650"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004156313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbgC/FadM family acyl-CoA thioesterase"
                     /protein_id="HNE01370.1"
                     /translation="MSTGPEASPHCSRIKVRGYHLDLYGHVNNARYLEFLEEARWAWF
                     ESGGELDRLAQRGLGFAIVRIDISYRRPAFMGEELEIRTALARIGRASGTLRQEVVLA
                     PEPAVRIAGADVTFCITDIAGGRALAFDAWLLALLGLPPAADGRRSDADDAGA"
     gene            2190..3065
                     /locus_tag="PLQ18_06655"
     CDS             2190..3065
                     /locus_tag="PLQ18_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008985421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNE01371.1"
                     /translation="MPGPEAARSLVPVLAGGGTRLPAHVGVLAALADFGISVPSYVGV
                     SGGSIVAALAAAGWPPPRLLRLALDTDFRRFRGRSLWQLARRGGLSSGEAFERWLDEL
                     LEGRRFRDLPKDLAVVATDVHTSQPVVFSRKTTPDERIARAVRCSMGIPLLFAFQPYR
                     DHLLVDGSILSEDALQRDWSGDGTPVCFFRLRGEPDTRPLSTAGLFPLPAYVSMLIRT
                     FMTTLSREFVDARHWPRTVVVEVGSYSPIDFALREADKHELYACGYETVRNILPFKFP
                     TLCPPPADAVLPDAS"
     gene            3188..>3553
                     /locus_tag="PLQ18_06660"
     CDS             3188..>3553
                     /locus_tag="PLQ18_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002811947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HNE01372.1"
                     /translation="MNRSSMTLHHARLWQGLGWALVAIVVWLSLDPSPPAPPDFLAWD
                     KAQHALAYGTLAWWFRQSFAPHRRWPLFLVGLGIGLEFLQGLGGVRMFDVDDMLANTV
                     GVGLGTALAVWTPAGRLLGW"
BASE COUNT          459 a         1305 c         1258 g          531 t
ORIGIN      
        1 gtgcggcgcg tgacgctgcc gaccggtgac agccggctgg cgatcaccgg cgtcagcacg
       61 cagatccagc ccgaaaccgc gctgctcgcc agcgaccggc cgctgctggt gctgtcgcag
      121 cactaccgca acgatgcgct gtccggcgac acgctgctcg aacgcttcgt cgggcgcgag
      181 atcgaggccg tgcgcgtcaa tccggccagc ggcgaggagc aacgcgagaa ggccgtgctg
      241 ctgtcggtgc gcgaggggca ggcggtgttc cgcatcggcg agcgcatcga atccgacggc
      301 gcgctgtcgc cgtggcggct ggtcgtcgag cgcggtcagg acgtgcgcga ggagccgacg
      361 ctgctcgccg acctcaacag cagcgtcggc ggcgagcacc tgatcgagct gtcctacctg
      421 tcgcgcggct tctcgtggga tgccgattac gtgctgcgcc tgaaccccgg caacgacggc
      481 atggacctgc tcgcctgggc gacgctgcac aacgccagcg gcacgacctt caccaatgcc
      541 cggctgtcgc tggtctccgg cgaggtcaac cgcatcgccg gcatgcgcgg cgaggctgcc
      601 gccgacctgc gctcggcgcc cgatctgccc ggcggcatgc cggggggagc ggccggcgat
      661 gaccggctgt tcgagtacca gctctatacg atcgaacggc cggtgacgct gccggcgcgc
      721 cagtcggtgc acgtgccgct gttctcgacc tcgcgcgccc cggtgcgcaa gctctaccgc
      781 acgaccgcgc cgctcgcgct gttcggtgcg gtgatcggca atcaggacct gcgcatcgcc
      841 acggcgctgt cgttcgcgaa cgtgtcgccg gggctcggcc ggccgctgcc ttcgggggcc
      901 gtgcgcgtct accagcgcga gccgagcggc accggccagt tcctcggcga ggcgcgcatc
      961 aatccggtcg ccgagggcga tgacgcgatc atcgaggtcg gcgccacgct cgatgtcagc
     1021 gcccgccgcc gccagatcga cttcaagcgc ctgccgcgcg acgagcagga agccgcgtgg
     1081 gtgatcgact tccgcaacac caagaccgag gcggtcaccg tcgatgtcac gcagcccttc
     1141 ggcggccagt ggcgtatcgt cgaggaaagc ctgccgcacc ggcgcgatgc cgccgacacc
     1201 gcgagctgga gcgtgccggt tccggccggc ggcagggtga cgctgagtta ccgcgcccgc
     1261 agccgctgag cgccgtcgcg ggcggcgcac gtaccgcccg tgcagaggcg ggggaggagg
     1321 atcgatggaa cgcgaactct gtgcgctgac cgacatcccc gatggcggca cccgcggcat
     1381 ccgcctgcag cccggcgcgc gctacgccga tctggtgctc gcgcgccgcg gcgcgcaggt
     1441 ctacgcctac cgcaactcct gtccgcatac cggcgcgccg atggagcgcg agtccggcga
     1501 cgacgatttc ctcgactatc agggtctgca catccagtgc ggcatccacg cggcgatctt
     1561 ccgcttcgag gacggcttct gcctgtgggg accgtgcgcg cgccggcggc tcgaagccgt
     1621 ggcggtgcgc atcgacgacg gtcgggtcat cgcgctcgac gtgcggccgt ggctgcgcga
     1681 ctgaggccgc gcgtgccaag ctgtcggttc cgccgccgat caggaagggc ctgccttgag
     1741 caccggaccc gaagccagcc cgcattgcag ccgcatcaag gtgcgcggct atcacctcga
     1801 cctgtacggg cacgtcaaca atgcccgcta cctcgaattc ctcgaagaag cccgctgggc
     1861 ctggttcgag agcggcggtg agctcgaccg gctcgcgcag cgcggcctcg gcttcgcgat
     1921 cgtgcgcatc gacatcagct accgccggcc ggccttcatg ggcgaggagc tcgaaatccg
     1981 cactgcgctc gcgcgcatcg gccgcgccag cggcacgctg cgtcaggagg tcgtgctcgc
     2041 accggagccg gccgtgcgca tcgccggggc cgacgtgacc ttctgcatca ccgacattgc
     2101 cggcggccgg gcgctggcct tcgatgcctg gctgctcgcg ctcctcggcc tgccgccggc
     2161 ggcggacggc cgccgcagcg atgccgacga tgccggggcc tgaggcggcg cgcagtctgg
     2221 tgccggtgct ggccggcggc ggcacgcggc tgccggcgca cgtcggcgtg ctcgccgcgc
     2281 tcgccgattt cggcatcagc gtgccgagct acgtcggggt gtccggcggc agcatcgtcg
     2341 cggcgctggc cgccgccggc tggccgccgc cgcggctgct gcgtctggcg ctcgacaccg
     2401 acttccggcg cttccgcggc cgctcgctgt ggcagctcgc gcgccgcggc gggctgtcct
     2461 cgggcgaggc cttcgagcgc tggctcgacg aactgctcga aggccggcgc ttccgcgatc
     2521 tgccgaagga cctggccgtg gtcgccaccg acgtgcacac ctcgcagccg gtggtgttca
     2581 gccgcaagac cacgccggat gagcgcatcg cgcgcgcggt gcgctgctcg atgggcatcc
     2641 cgctgctgtt cgcgttccag ccgtatcgtg accacctgct ggtcgacggc agcatcctgt
     2701 ccgaggatgc gctgcagcgc gactggagcg gcgacggcac gccggtgtgc ttcttccggc
     2761 tgcgcggcga gcccgacacc cggccgctgt ccacggccgg actgtttccg ctgccggcct
     2821 acgtgtcgat gctgatccgc accttcatga ccacgctgtc gcgcgagttc gtcgatgccc
     2881 gccactggcc gcgcaccgtc gtcgtcgagg tcggcagcta ctcgccgatc gacttcgcgc
     2941 tgcgcgaggc cgacaagcac gagctgtatg cctgcggcta cgagacggtg cgcaacatcc
     3001 tgccgttcaa gttcccgacg ctgtgcccgc cgcccgcgga cgccgtcctg cccgatgcct
     3061 cctgaagcgg cgggcgcgac gcgcggccgg ccgcggcggc cggcaggccg gcctgatccg
     3121 gctcgccggc aatgccactc acaacgcatt cacgaacgcc ttcacgcatg accggtagag
     3181 accccgcatg aaccgaagct ccatgacgct gcaccacgcc cgcctgtggc agggcctcgg
     3241 ctgggcgctg gtcgcgatcg tcgtctggct gtcgctcgat cccagtccgc cggcgccgcc
     3301 ggatttcctc gcctgggaca aggcccagca cgccctcgcc tacggcacgc tggcctggtg
     3361 gttccgccag agcttcgcgc cgcaccggcg ctggccgctg ttcctggtcg ggctcggcat
     3421 cgggctggag ttcctgcagg ggctcggcgg cgtgcgcatg ttcgatgtcg acgacatgct
     3481 cgcgaacacc gtcggcgtcg gtctcggcac ggcgctcgcc gtgtggacgc cggccggccg
     3541 gctgctcggc tgg
//
LOCUS       DAOQJH010000245         3546 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_16734_length_3546_cov_3.130622, whole genome shotgun sequence.
ACCESSION   DAOQJH010000245 DAOQJH010000000
VERSION     DAOQJH010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3546)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3546)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3546
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16734_length_3546_cov_3.130622"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..406)
                     /locus_tag="PLQ18_06665"
     CDS             complement(<1..406)
                     /locus_tag="PLQ18_06665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE01373.1"
                     /translation="MSRALERFYRGLYVEHVDTAGFLYEQWATRLMESAPAARALVEL
                     ECRLEPHLDALLLGGERALAVALEAAAGGEPGERHIAARLFCRLRHAAGLRATLDGYD
                     ALAADDAAAAAGLRTALARALAWELPGDWLAPL"
     gene            complement(403..1470)
                     /locus_tag="PLQ18_06670"
     CDS             complement(403..1470)
                     /locus_tag="PLQ18_06670"
                     /inference="COORDINATES: protein motif:HMM:NF021456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2169 domain-containing protein"
                     /protein_id="HNE01374.1"
                     /translation="MIPAPLTPELDDRSGHALAPLAAADAEGRPVLALVVQATFALFP
                     DLAGRAPPLAPLQRPIALAGEHTGEPGRSSLRREPETAWFKPGTDLVLLGHAQAPGGE
                     PVTQLDAGLRVGATQKIVRVFGDRAWTDTGAGLSVSGPQPFQRLPLVWERAFGGVDAA
                     DAQRRCEPRNPVGCGFVAPGSRLAGPQRMPNLEDPAAPLRQPGDCPPPAGFGFIAPHW
                     QPRAALAGTYDAGWQASRAPRLPADFQPAFLNAAAPGLVVPGRLRGDEPVIVLNAAAV
                     PRLAFALPGYPPPPLRIDWRKGGHVRLDAQLDTVVIDTDAMQLLLTWRAHVVLPARGL
                     HAVAAIRARAGRPQALAGARP"
     gene            complement(1470..2273)
                     /locus_tag="PLQ18_06675"
     CDS             complement(1470..2273)
                     /locus_tag="PLQ18_06675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE01375.1"
                     /translation="MDPRDARPLIAGAIEEDLTLYAVGVPAGELPGLFGRVSENYQAL
                     GIAILLASGEQARYRECLVRSAWARLHQLRCAGALQPPARERATSFGEGLFDALAAGH
                     WRLAHDIAEACPPGWIPEGEYEDDFCHMRLIGLYTGDSPPEPAAVQALLARHAQVLDG
                     ARTPRLALGQALFDHDADAFAAAFEDFLREELEARMEARERLGEPDADAARVWMRGYV
                     ATEALALLRVAETAGIAPAGPHPLCPPGSLPAGPCPNFPDPFAEIEAAR"
     gene            complement(2275..2880)
                     /locus_tag="PLQ18_06680"
     CDS             complement(2275..2880)
                     /locus_tag="PLQ18_06680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE01376.1"
                     /translation="MSEYKPEFVIWTSRYVEGWCTLLRPEPYEDLYELDYGQPLADRW
                     PADSVSRMDPDRPKDLQLADSVRCPYARTVSTRLRAALEAAGETRLEFLPLAILNHKG
                     RVAARDYWIVNPPYTLDCIDLDASNARWAGRMLDADDPFTACDRLVLRPEVVPAGVQV
                     FRARFKPSLLLMRRALAEALLNEGLSGLAFGEPDAYRGLAA"
     gene            complement(2912..>3546)
                     /locus_tag="PLQ18_06685"
     CDS             complement(2912..>3546)
                     /locus_tag="PLQ18_06685"
                     /inference="COORDINATES: protein motif:HMM:NF025769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AHH domain-containing protein"
                     /protein_id="HNE01377.1"
                     /translation="FKRGRTQRGHIDSCASGYTQTAKNRFRQVHHVVPVSSCSDATIS
                     KYVTAAKLKLLHNCMAETDWKIDAAKNVISLPLKPVYLDKRAPAGWDKLPCHQVEHNP
                     AYTDAVSDYLKDNVWNKVQKQAKSCELDPEDLKKKMEDASDHWRDFLTDRGKEHGGTK
                     KCWDKQMSMPDTWYIPFSMNPGTPTPRAPVKWDDLSGSIKEKLKQLFQLH"
BASE COUNT          475 a         1252 c         1327 g          492 t
ORIGIN      
        1 acagcggcgc cagccagtcg ccgggcagct cccaggccag cgcccgcgcc agcgcggtgc
       61 gcaagcccgc ggccgccgcc gcatcgtcgg cggcgagcgc gtcatagcca tcgagcgtgg
      121 cacgcaggcc ggcggcatgg cgcagccggc agaacagccg cgcggcgatg tgacgctcgc
      181 cgggctcgcc gccggcagcg gcttcgagtg cgacggcgag cgcgcgctcg ccgccgagca
      241 gcagcgcatc gaggtgcggc tccagccggc attcgagctc gaccagcgcg cgcgcggccg
      301 gcgccgattc catcagccgc gtcgcccact gttcgtacag gaagccggcg gtgtcgacgt
      361 gctcgacgta cagcccgcgg tagaagcgtt cgagtgcccg gctcatgggc gtgctcccgc
      421 cagcgcctgc ggccggccgg cgcgcgcgcg gatcgccgcg acggcatgga ggccgcgcgc
      481 cggcagcacg acgtgcgcgc gccaggtcag cagcagctgc atcgcgtcgg tatcgatgac
      541 gacggtgtcg agctgcgcat cgaggcgcac atgcccgccc ttgcgccagt cgatgcgcag
      601 cggcggcggc gggtagccgg gcagcgcgaa ggcgagccgc ggcaccgcgg cggcgttcag
      661 cacgatcacc ggctcgtcgc cgcgcagccg cccgggcacg acgaggcccg gcgcggcggc
      721 gttcaggaag gccggctgga agtccgccgg cagccgcggg gcgcggctcg cctgccaccc
      781 ggcgtcatag gtgccggcga gtgccgcgcg cggctgccag tgcggggcga tgaagccgaa
      841 gccggccggc ggcggacagt cgccgggctg gcgcagcggc gcggccgggt cttcgaggtt
      901 cggcatgcgc tgcgggccgg cgagccggct gcccggtgcg acgaaaccgc agccgacggg
      961 gttgcgcggc tcgcagcggc gctgcgcatc ggcggcatcg acgccgccga aggcgcgctc
     1021 ccagacgagc ggcaggcgct ggaagggctg cggaccgctg accgacaggc cggcgccggt
     1081 gtcggtccag gcgcggtcgc cgaacacgcg cacgatcttc tgcgtggctc cgacgcgcag
     1141 gccggcatcg agttgcgtga ccggctcgcc gccgggcgcc tgcgcatggc cgagcagcac
     1201 gagatcggtg ccgggcttga accaggcggt ctcgggttcg cggcgcaggc tggagcgccc
     1261 gggctcgccg gtgtgctcgc cggcgagcgc gatcgggcgc tgcagcggcg cgagcggcgg
     1321 ggcgcgaccg gcgaggtccg ggaacagcgc gaaggtcgcc tgcaccacca gcgccagcac
     1381 cggccggccc tcggcatcgg cggcggcgag cggtgccagc gcgtggccgc tgcggtcgtc
     1441 gagctcgggc gtcagcgggg ccgggatcat cagcgtgcgg cctcgatttc agcgaagggg
     1501 tccgggaagt tcgggcaggg cccggccggc agcgagccgg gcggacagag cggatgcgga
     1561 ccggccggag cgatgccggc ggtttcggcc acgcgcagca gcgccagcgc ctcggtcgcg
     1621 acgtagccgc gcatccacac ccgcgccgcg tccgcgtccg gctcgccgag gcgctcgcga
     1681 gcttccatgc gcgcttcaag ctcctcgcgc aggaaatcct cgaaggccgc cgcgaaggca
     1741 tcggcgtcgt gatcgaacag cgcctgcccg agtgcgagcc gcggcgtgcg cgcgccgtcg
     1801 agcacctgcg cgtgacgcgc gagcagcgcc tgcacggcgg ccggctccgg cgggctgtcg
     1861 ccggtgtaca agccgatcag ccgcatgtga cagaagtcgt cctcgtactc gccttcgggg
     1921 atccagcccg gcgggcaggc ttcggcgatg tcgtgggcca gccgccagtg gccggcggcg
     1981 agcgcatcga acaggccctc gccgaagctg gtcgcacgct cgcgcgccgg cggctgcagc
     2041 gcgcccgcgc agcgcaactg gtgcagccgc gcccaggcgc tgcgcaccag gcactcgcgg
     2101 tagcgcgcct gttcaccgct cgcgagcagg atggcgatgc cgagcgcctg ataattctcc
     2161 gatacccggc cgaacagccc gggcagctcg ccggccggca cgccgacggc atagagcgtc
     2221 aggtcctcct cgatcgcgcc ggcgatcagc ggccgtgcat cgcgcgggtc catgtcaggc
     2281 cgccagcccg cggtaggcgt ccggttcgcc gaaggcgagg ccgctcaggc cctcgttcag
     2341 cagcgcttcg gccagcgcac ggcgcatcag cagcaggctc ggcttgaagc gcgcgcggaa
     2401 cacctgcacc ccggccggca cgacctcggg gcgcagcacc agccggtcgc aggccgtgaa
     2461 cggatcgtcg gcgtcgagca tcctgccggc ccagcgggcg ttcgaggcgt cgaggtcgat
     2521 gcagtcgagc gtgtacggcg gattgacgat ccagtagtcg cgcgccgcga cccggccctt
     2581 gtggttgagg atggcgagcg gcaggaattc gagccgcgtc tcgccggcgg cttcgagcgc
     2641 agcgcgcagc cgcgtcgaga ccgtgcgcgc atacgggcag cgcacgctgt cggcaagctg
     2701 caggtccttc ggccggtccg ggtccatgcg gctgaccgaa tcggccggcc agcgatcggc
     2761 gagcggctgg ccgtagtcga gctcgtagag atcctcgtag ggctcgggcc gcagcagcgt
     2821 gcaccagccc tcgacgtagc ggctggtcca gatcacgaac tcgggcttgt attcggacat
     2881 gcgtgcctgc ctccccgcgc cgggctggct gtcaatgcag ctggaacagc tgcttgagct
     2941 tttccttgat gctgccgctc aggtcatccc acttcaccgg cgcacgcggc gtcggcgtgc
     3001 ccgggttcat cgagaacggg atgtaccagg tgtccggcat gctcatctgt ttgtcccagc
     3061 acttcttggt gccgccgtgc tccttgccgc gatcggtcag gaagtcgcgc cagtgatcgc
     3121 tcgcgtcctc catcttcttc ttcaggtcct cggggtcgag ctcgcagctc ttggcctgct
     3181 tctggacctt gttccagacg ttgtccttca gatagtcgct gacagcatcg gtataggccg
     3241 gattgtgctc gacctgatgg cagggcagct tgtcccagcc ggccggcgcg cgcttgtcga
     3301 gatacacggg tttcagcggc agcgagatga cgttcttggc agcgtcgatc ttccagtcgg
     3361 tctcggccat gcagttgtgc agcagcttga gcttggcggc cgtcacgtac ttcgagatcg
     3421 tcgcgtccga gcagctgctg accggcacga cgtgatgcac ctgccggaag cggttcttcg
     3481 cggtctgcgt gtagccgctc gcgcagctgt cgatgtggcc gcgctgcgtg cggccgcgct
     3541 tgaact
//
LOCUS       DAOQJH010000246         3535 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_16809_length_3535_cov_3.630747, whole genome shotgun sequence.
ACCESSION   DAOQJH010000246 DAOQJH010000000
VERSION     DAOQJH010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3535
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16809_length_3535_cov_3.630747"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..673)
                     /gene="secD"
                     /locus_tag="PLQ18_06690"
     CDS             complement(<1..673)
                     /gene="secD"
                     /locus_tag="PLQ18_06690"
                     /inference="COORDINATES: protein motif:HMM:NF025098.2"
                     /note="part of the preprotein secretory system; when
                     complexed with proteins SecF and YajC, SecDFyajC
                     stimulates the proton motive force-driven protein
                     translocation, and appears to be required for the release
                     of mature proteins from the extracytoplasmic side of the
                     membrane; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HNE01378.1"
                     /translation="MNQFAWWKNLLIVLVCVIGALYALPNVFGEDPAVQISATRAAPV
                     DQNLIERVGSELQGLKIQPKTFELENGRLLVRLTGTDDQLRAADRLGQALGNDYIVAL
                     NLAPATPQWLRNLRASPMFLGLDLRGGVHFLMEVDMKAVVKQLEERYQEEIRTLLRTE
                     KIRYNAVGRAQGGGVKVSFRDGTDAQKGLGVIREKLPALVPGPASSDGTELSFQFSEK
                     ELADKQ"
     gene            complement(806..1138)
                     /gene="yajC"
                     /locus_tag="PLQ18_06695"
     CDS             complement(806..1138)
                     /gene="yajC"
                     /locus_tag="PLQ18_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005963882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HNE01379.1"
                     /translation="MSFLISDAYAQAAPAGAASDPIMQFLPLVVIMVAFYFILLRPQQ
                     KRQKEHKSMIEALKKGDEVVTGGGVLGRIVEVGDNFVSLEVADKVEIKVQKAAVSSLL
                     PKGTVKGL"
     gene            complement(1274..2401)
                     /gene="tgt"
                     /locus_tag="PLQ18_06700"
     CDS             complement(1274..2401)
                     /gene="tgt"
                     /locus_tag="PLQ18_06700"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019583635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HNE01380.1"
                     /translation="MEFQLFTTDGAARRGRMTFARGTVETPAFMPVGTYGTVKAMTPE
                     EIREIGAEIILGNTFHLMLQPGTDVIGAHGDLHDFMHWSGPILTDSGGFQVFSLAELR
                     KISEQGVTFRSPVNGDKVFLGPEESMQVQRALGSDIVMIFDECTPYPATETEARKSME
                     LSLRWAKRSRAEHDRLANPNALFGIVQGGMYPVLRTQSAMGLAEIGFDGYAVGGLAVG
                     EPMAERNHILEHTVPQLPVERPRYLMGVGKPEDIVEAVARGIDMFDCVMPTRNARNGH
                     YFTWDGDVRIRNSRHRLDTGPISHDCDCYACRHYSRAYLKHLDRNQEILGARLATIHN
                     LHFYQQLMARIRAAISEGCFEEFRAGFHARRGGGAAADGCV"
     gene            complement(2943..>3535)
                     /locus_tag="PLQ18_06705"
     CDS             complement(2943..>3535)
                     /locus_tag="PLQ18_06705"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNE01381.1"
                     /translation="GSRTRHHTVAHGKSRVLLGRFTSSVECAAPLRTISSESTMKSSV
                     YIETSVLSYLMARPSNDLRAMANQSVTAEWWETQKDHYSLVASELVISEAGRGHPEAA
                     QRRLAVVNSLPLLQVSQQAQELAQALIQEYALPQKAEGDALHVALAAVNGVEYLLTWN
                     CTHIANAHTRPKIEATCRAHGYEPPVICTPLELMEN"
BASE COUNT          564 a         1106 c         1201 g          664 t
ORIGIN      
        1 tctgcttgtc ggcgagttcc ttctccgaga actggaagct cagctccgtg ccatccgacg
       61 aagccggccc cggcaccagc gccggcagct tctcgcggat cacgccgagg cccttctgcg
      121 catcggtgcc gtcgcggaag ctgaccttca cgcccccgcc ctgcgcacgg ccgaccgcgt
      181 tgtagcggat cttctcggtg cgcagcagcg tgcggatttc ctcctgatag cgctcttcga
      241 gctgcttcac gaccgccttc atgtccactt ccatcaggaa gtgcacgccg ccgcgcaggt
      301 cgaggccgag gaacatcggg cttgcgcgca ggttcctgag ccactgcggc gtggccgggg
      361 ccaggttcag cgcgacgatg tagtcgttgc cgagcgcctg cccgagccgg tcggcggcgc
      421 gcagctgatc gtcggtgccg gtcaggcgca cgagcaggcg gccgttctcg agctcgaagg
      481 tcttcggctg gatcttcagc ccctgcagct cgctgccgac gcgctcgatc aggttctggt
      541 cgaccggcgc ggcgcgggtc gccgagatct gcacggcggg gtcttcaccg aaaacgttgg
      601 gcagggcgta cagcgcgccg atcacgcaca cgagcacgat cagcaggttc ttccaccagg
      661 cgaactggtt catcgacgac ggctcccggc cggagcgcag cgcgcatccg gaaaacgacc
      721 cgaccgcgcc ggcggccgcg ccgtcgtccg gtcctgcgga cgacggcggg ccgccggcgc
      781 ggtcgcgagg ctggctggct ggggctcaga ggcccttgac cgtgcccttg ggcagcagcg
      841 agctgacggc agccttctgc accttgatct cgaccttgtc ggcgacttcg agcgacacga
      901 agttgtcgcc aacctcgacg atgcgcccga gcacaccgcc gccggtgacg acctcgtcac
      961 ccttcttcag cgcctcgatc atgctcttgt gctccttctg ccgcttctgc tgcggacgca
     1021 gcaggatgaa gtagaaggcg accatgatca cgaccagcgg caggaactgc atgatcggat
     1081 cgctggcggc gccggcgggg gcggcctggg cgtaggcgtc ggaaatcagg aagctcatgg
     1141 actcactttc tccgtggggt cggttcgttg tgctggccgc tcgtccggtc gggggcggct
     1201 ctggcgccgg cagcttgtgc ctgccggcac cggtcgccgc ccgtcgggac ggcggccggc
     1261 aaaaggtcgc gcattataca cagccgtccg cagccgcccc gccgccacgg cgggcgtgga
     1321 agccggcgcg gaattcctcg aagcagccct cgctgatcgc ggcgcggatg cgtgccatca
     1381 gctgctggta gaagtgcagg ttgtggatgg tggcgaggcg cgcgccgagg atttcctggt
     1441 tgcggtcgag gtgcttcaga tacgcgcgcg agtagtgccg gcaggcgtag cagtcgcagt
     1501 cgtggctgat cgggccggtg tcgagccggt ggcggctgtt gcggatgcgc acgtcgccgt
     1561 cccacgtgaa gtagtggccg ttgcgggcgt tccgcgtcgg catcacgcag tcgaacatgt
     1621 cgatgccgcg cgccaccgcc tcgacgatgt cctcgggctt gccgacgccc atcaggtagc
     1681 gcggccgctc caccggcagt tgcggcacgg tgtgttcgag gatgtggttg cgctcggcca
     1741 tcggctcgcc gaccgcgagc ccgccgaccg cgtagccgtc gaagccgatc tcggccagcc
     1801 ccatcgccga ctgcgtgcgc agcaccggat acatgccgcc ctgcacgatg ccgaacagcg
     1861 cgttcgggtt ggccaggcgg tcgtgctcgg cgcgcgatcg cttcgcccag cgcagcgaca
     1921 gttccatgga cttgcgcgct tcggtctcgg tcgccgggta gggcgtgcat tcgtcgaaga
     1981 tcatgacgat gtcggagccg agcgcgcgct gcacctgcat ggattcctcg ggaccgagga
     2041 acaccttgtc gccgttgacc ggcgagcgga aggtcacgcc ctgctcgctg atcttgcgca
     2101 gctccgcgag cgagaacacc tgaaagccgc cggagtcggt caggatcggc ccgctccagt
     2161 gcatgaagtc gtgcaggtcg ccgtgggcgc cgatcacgtc ggtgccgggc tggagcatca
     2221 ggtggaaggt gttgccgagg atgatctcgg cgccgatctc gcggatttcc tccggcgtca
     2281 tggccttgac ggtgccgtag gtgccgaccg gcatgaacgc cggggtttcg accgtaccgc
     2341 gcgcaaacgt catccgcccg cgccgcgcgg cgccgtcggt cgtgaacaac tggaattcca
     2401 tggggatgcg gccggccggt gaagggcgcg acagtttcgt gaccgcaacc gggcttggca
     2461 agccggttac cgcggacggc gcggcaaggc tgcacatcct tgtgtgttga cggtccaggc
     2521 tgatcttgcg ggaccgtggc cgcggttcac ggtccgcgcc gcttcctcgc cgcaaccgaa
     2581 ggcgctgcga ggcaggcgca ggggcatgag caaggcagcg agcgcagggg atgacggtga
     2641 ttgccgtgaa tctgcaggcg atgaacagaa tcaacgaaga tgttgcggat tcgtgcgcac
     2701 gcggattttg aaagcttgta cggcgtgcgc atgaatgcgc agcctacccg gcgggagcgt
     2761 cttgttgggg agccactgtg ttgaccgccg gtttacgcgg agggaaggac accatgcgct
     2821 cgggatgagc cgcttgatgc gtcatgaggt ctttacagat agcctcaatg tcatagttga
     2881 actggcgtgc atactcgtct ctaagtgcgc gtgtttctga aacaacaggg tcttgccaca
     2941 tatcagttct ccatgagttc aagcggagtg cagatgactg gtggttcgta gccgtgcgct
     3001 ctgcaggttg cttcgatttt aggccgcgta tgcgcgttgg caatatgcgt gcaattccag
     3061 gtgagaagat actcaacgcc gtttacggca gccagcgcaa cgtgcagggc atctccttct
     3121 gctttttgcg gcagggcgta ttcctggatc aaggcttgcg ccaactcctg cgcctgctga
     3181 gaaacttgca gcagtggcag gctgttgaca acagcaagac gccgctgcgc cgcttctgga
     3241 tgaccgcgtc cggcttctga aataaccagt tcagaagcca ccaggctgta atggtctttt
     3301 tgtgtttccc accattccgc cgtgacactt tggttggcca tggcacgcag gtcgttgctt
     3361 ggacgtgcca tcaagtagct cagaacggat gtttcgatgt aaacagatga cttcattgtg
     3421 ctctcagagc tgatggtgcg cagcggagct gcacactcta cggaactggt gaatctgccg
     3481 agaagaaccc gtgactttcc gtgggcgacc gtgtgatgtc ttgtccgaga gccgg
//
LOCUS       DAOQJH010000247         3522 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_16925_length_3522_cov_4.214306, whole genome shotgun sequence.
ACCESSION   DAOQJH010000247 DAOQJH010000000
VERSION     DAOQJH010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3522
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16925_length_3522_cov_4.214306"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /locus_tag="PLQ18_06710"
     CDS             complement(<1..245)
                     /locus_tag="PLQ18_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018718723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HNE01382.1"
                     /translation="MIRTPIAFIGGGNMARSLIGGLLADGCPATLLRVAEPSAGGREA
                     LRAQYGVQTSEHNPDVIDGAGVVVLAVKPQILREVALG"
     gene            complement(337..2067)
                     /locus_tag="PLQ18_06715"
     CDS             complement(337..2067)
                     /locus_tag="PLQ18_06715"
                     /inference="COORDINATES: protein motif:HMM:NF024901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNE01383.1"
                     /translation="MSVRMMALALIVALGPALPAAAAPPMMKWQDEQGRWHYGDRPPQ
                     AGARADALLETGQDAAAAKEYETWAKMGDARAQTKLGELYLEGRGVSRDEAKARELFG
                     IAAGNGYAEARFRLGDLLATGRGGALDLPGAVRWYRQAAEGGHAGAQGRYGTALVDGQ
                     GIARDVAQGLQWLMRGAEAGDADAEYRLGLCYLRGEGVLGDPRTAVEWFRRAATRGQP
                     GAAQQLAELAASGRVPGLELAEVMPGLVRAAESGFPPAQLSLGLIHLQGLTGTQNLAL
                     AVRWLGAAAEAREPLAQFTLAGLYANGEGVARDERRAVQLYRGAAEAGHPRAQFELAE
                     RLAKGAGVPRDDAEAVVWYARAAKQGLASAHTRLGEHYQRGRGIAADLLLAVREYRAA
                     AEAGHAPAQLALALLTRAGTGTERDPELAVLWLRRAADQGLGLAQVDLGLAYAEGSGV
                     ARDDEQAARWYARAARAGYAPGRFLLGMASLTGRGVQRDEAQAAVLLRAAAEQGYAPA
                     QTAYGLLLRDGRGMPADPVAAWKWLDLAALDGDQVALQARETLAARLPPAQLQGLRRD
                     ARLWRPKTGP"
     gene            complement(2064..2405)
                     /locus_tag="PLQ18_06720"
     CDS             complement(2064..2405)
                     /locus_tag="PLQ18_06720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE01384.1"
                     /translation="MRARRSACTGALMLVLALAAAPGALADDAAFAALLTAASRNLQS
                     PAAPFEEEDGTPPNLDAYGFDNWLAREQPGLAREYTRLDADARRQVYARYRAEPTLAA
                     IRDSILARSRP"
     gene            complement(2402..3109)
                     /locus_tag="PLQ18_06725"
     CDS             complement(2402..3109)
                     /locus_tag="PLQ18_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011628092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HNE01385.1"
                     /translation="MSSTPDPGLDERLARVLDRIRSAELRYGRTGGSVGLLAVSKTQP
                     AAAVRALAALGQSAFGENYLQEALDKRSELADLPLAWHFIGPVQSNKTRRLAEAFDWV
                     HAVDRLKIAERLSAQRPVQLPPLSVCIEVNVDGEASKSGVTPAALPELAHAVAALPNL
                     RLRGLMAIPAPAADFDAQRRPHARLRQAFEALQHDGLALDTLSIGMSDDLEAAIAEGA
                     TLVRIGTAIFGPRRAPA"
     gene            3234..>3522
                     /locus_tag="PLQ18_06730"
     CDS             3234..>3522
                     /locus_tag="PLQ18_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008734599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twitching motility protein PilT"
                     /protein_id="HNE01386.1"
                     /translation="MNIDELLAFGVKHNASDLHLSAGLPPMLRVDGDVRRINLPPMDH
                     KEVHGMIYDIMNDRQRKEYEENLETDFSFAIPGLARFRVNAFNQNRGAGAVF"
BASE COUNT          470 a         1344 c         1253 g          455 t
ORIGIN      
        1 ccgagggcga cctcgcgcag gatctgcggc ttgacggcga gcacgacgac gccggcgccg
       61 tcgatcacgt cggggttgtg ctcgctggtc tgcacgccgt actgcgcgcg cagcgcctcg
      121 cgaccgccgg ccgagggttc ggccacacgc agcagggtgg ccggacagcc gtcggcgagc
      181 aggccgccga tcaggctgcg ggccatgttg ccgccgccga tgaaggcgat gggggtgcgg
      241 atcatgcagc aaggcctcgg gcgtcgtacg ggaaccgcgc aaagctaagc gcggtccggc
      301 gaatccgcaa ccgcgcgccg cccggccgcg cctgcttcag gggccggtct tcgggcgcca
      361 gagccgcgca tcgcgccgca ggccctgcag ttgcgccggc ggcagccgcg cagccagcgt
      421 ctcgcgcgcc tgcagcgcca cctgatcgcc gtcgagggcg gccagatcca gccatttcca
      481 cgccgccacc ggatcggcgg gcatgccgcg gccgtcgcgc agcagcaggc cgtaggcggt
      541 ctgcgccggc gcatagccct gctcggccgc tgcgcgcagc agcacggcgg cctgcgcctc
      601 gtcgcgctgc acgccccgcc cggtcagcga cgccatgccg agcaggaagc gcccgggcgc
      661 gtagccggcg cgcgcggccc gcgcatacca gcgcgccgcc tgctcgtcat cgcgcgccac
      721 gccgctgcct tcggcgtagg caaggccgag atcgacctgc gccagcccga gcccctgatc
      781 ggccgcccgg cgcagccaca gcacggccag ttcgggatcg cgctcggtgc cggtgccggc
      841 gcgcgtcagc agcgccagcg caagctgcgc cggcgcatgg ccggcctcgg ccgcggcccg
      901 gtattcgcgc acggccagca gcagatcggc ggcgatgccg cgcccgcgct ggtagtgctc
      961 gccgagccgg gtatgggcgc tggcaagccc ctgcttggcg gcacgtgcgt accagaccac
     1021 ggcctcggca tcgtcgcgcg gcacgccggc gcccttcgcc agccgctcgg ccagctcgaa
     1081 ctgcgcgcgc ggatggccgg cctcggccgc cccgcggtag agctgcacgg cgcggcgctc
     1141 gtcgcgggcc acgccctcgc cgttcgcata cagcccggcc agcgtgaact gcgccagcgg
     1201 ctcgcgcgcc tccgccgcgg ccccgagcca gcgcacggcc agcgccagat tctgcgtgcc
     1261 ggtcagtccc tgcagatgga tcaggccgag cgacagctgc gccggcggaa agccgctttc
     1321 ggcggcgcgc accagaccgg gcatcacctc ggccagctcc agccccggca cccggccgct
     1381 ggctgcgagt tcggcgagct gctgcgcggc gccgggctgg ccgcgggtcg cggcgcggcg
     1441 gaaccattcg accgccgtgc gcggatcacc gagcacgccc tcgccgcgca gatagcagag
     1501 accgagccgg tattcggcat cggcgtcacc ggcctcggca ccgcgcatca gccactgcag
     1561 gccttgcgcg acatcgcgcg cgatgccctg gccatcgacc agcgccgtgc cgtagcggcc
     1621 ctgcgcgccc gcatgcccac cctcggccgc ctgccggtac cagcgcaccg cgccgggcag
     1681 gtcgagcgcg ccgccgcgcc cggtcgcgag cagatccccg agccggaagc gggcctcggc
     1741 atagccgttg ccggcggcga tgccgaacag ctcgcgcgcc ttcgcctcgt cgcgcgacac
     1801 cccgcggcct tcgaggtaca gctcgccgag cttggtctgg gcgcgggcgt cgcccatctt
     1861 cgcccaggtt tcgtattcct tcgccgccgc ggcgtcctgg ccggtttcga gcagcgcatc
     1921 ggcccgggcc ccggcctgcg gcggccggtc gccgtagtgc cagcggccct gttcgtcctg
     1981 ccatttcatc atcggcggcg cagccgcggc cggcagcgcc ggaccgagtg cgacgatcag
     2041 ggccagcgcc atcatgcgca cgctcatggc cgggaccgcg caaggatcga atcgcggatc
     2101 gccgcgagcg tcggctcggc ccgatagcgc gcatagacct gccggcgggc atcggcatcg
     2161 agccgggtgt actcgcgcgc cagaccgggc tgctcgcggg ccagccagtt gtcgaagccg
     2221 taggcatcga ggttgggcgg cgtgccgtcc tcctcctcga agggcgcagc cggcgactgc
     2281 aggttgcggc ttgcggccgt gagcagcgca gcgaacgcgg cgtcatcggc cagcgccccg
     2341 ggcgcggcgg cgagcgcgag caccagcatc agcgcgcccg tgcaggcaga gcgccgcgcc
     2401 ctcatgccgg cgccctccgc ggcccgaaga tcgccgtgcc gatgcgcacc agcgtcgcgc
     2461 cctcggcgat cgcggcttcg agatcatcgg acatgccgat cgacagcgtg tcgagcgcga
     2521 gcccgtcgtg ctgcagcgcc tcgaaagcct gacgcaggcg cgcgtgcggc cggcgctgcg
     2581 catcgaaatc cgccgccggc gccgggatcg ccatcagtcc gcgcaggcga aggttcggca
     2641 gcgccgcgac cgcgtgcgca agctccggca gtgcggccgg cgtcaccccg ctcttgctgg
     2701 cttcgccgtc gacgttgacc tcgatgcaca ccgacagcgg cggcagctgc accggtcgct
     2761 gcgccgacag acgctcggcg atcttcaggc gatcgactgc atgcacccag tcgaaggctt
     2821 cggcgagccg gcgggtcttg ttcgactgca ccggcccgat gaaatgccag gcgagcggca
     2881 ggtcggcgag ctcgctgcgc ttgtccaggg cttcctgcag gtagttctcg ccgaaggcgc
     2941 tctggccgag tgcggcgagt gcgcgcacgg ccgcggctgg ctgggtcttg ctgacggcga
     3001 gcagcccgac gctgcccccc gtgcggccgt agcgcagctc ggcgctgcgg atgcggtcga
     3061 ggacgcgcgc gaggcgctcg tccaggcccg gatcgggggt actgctcatg cggggactcc
     3121 ggtcggccgt ggctcggctc tgcttgcgat cggcttgcta tagtgcggcg cacttcccgt
     3181 atccagtcac acccggtcac gcacaacgtc acgtacacac ggagcggccg cgcatgaaca
     3241 tcgacgaact gctggccttc ggggtcaagc acaacgcctc ggacctgcac ctgtccgcgg
     3301 gcctgccgcc gatgctgcgt gtcgatggtg acgtgcggcg catcaacctg ccgccgatgg
     3361 accacaagga agtgcacggg atgatctacg acatcatgaa cgaccgccag cgcaaggagt
     3421 acgaggagaa cctcgaaacc gacttctcct tcgcgatccc cggcctcgcg cgcttccgcg
     3481 tcaacgcctt caaccagaac cgcggcgccg gcgcggtgtt cc
//
LOCUS       DAOQJH010000248         3520 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_16939_length_3520_cov_3.548629, whole genome shotgun sequence.
ACCESSION   DAOQJH010000248 DAOQJH010000000
VERSION     DAOQJH010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3520)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3520)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3520
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16939_length_3520_cov_3.548629"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(263..2695)
                     /locus_tag="PLQ18_06735"
     CDS             complement(263..2695)
                     /locus_tag="PLQ18_06735"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HNE01387.1"
                     /translation="MSHRFARTAGRTLAVLTALLALGIAGGWYWLRGSLPPLAGELSL
                     PGLQAPVSIVRDAAAVPHIRAQNVHDAWFALGVVHAQDRLWQMEMSRRIAGGRIAEAL
                     GATALDTDRLLRTMGLRRVAERNYRNLDAESRAVLDAYAAGVNAWIAAHGGPLPPEFL
                     LTGVRPEAWQPADSLSWLKVMALDLGSNWSGELTRLRLARQLPTARIQDFLPPVPYAG
                     PLPVADYAALYRGLDLPLAALGPQPRTDTDGLGSNNWVVAGSRSASGKPLLANDPHLG
                     LTAPAIWYFAHLEAPGLRVIGATLPGVPMVVIGRNERIAWGLTNTNPDVQDLYVERSD
                     PADPGRYQTPDGWQRFEQREEILHVRNAPDERLIVRTSRHGPLLPDTRTGDALPAGYG
                     LALAWTALAEDDASFRAASKLAYAQDWTGFNEALREFVAPQQNFVYADVDGHIGFLSP
                     GRIPVRRPDNDLLGLAPAPGWEARYDWAGWVPYEELPRVFDPADGALYSANERIVPPG
                     YRWHLTDEWQPAYRAARIRELLAATPAHTPASFRQMHADIRSGLAADLLPKLLQRAAA
                     TDYPPAARGWHERLARWDYGMRVDAAEPLVFSAWYRALTRRIYADELGPEFERLWDQR
                     TTFMNNVLDDRDGQGLWCDDVRTAATESCAQQVVAALGEALDELHARFGSDESRWHWG
                     DAHPAVAEHRPFSKLAWLRPLFELRRPVGGDVSTLDVARFFINDVEAPYAARHVASLR
                     MIVDLADPDRSTFMHSSGQSGNPLSPHYADLLDRWTRVESLPMSMQARDYEAGALGTL
                     TLRPPAAVSGGQ"
     gene            2978..>3520
                     /locus_tag="PLQ18_06740"
     CDS             2978..>3520
                     /locus_tag="PLQ18_06740"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNE01388.1"
                     /translation="MSPSAETAAAVPSTGIFEAVEPARAWTMVAGAIEQLCARSPAAV
                     PLAELAAAAGLSEGQFQRVFSAWAGISPKRFQQFLGKEYAKQALRAGQDVLNAALAAG
                     LSGPGRLHDLLVSAEAVSPGEWRAGGSGLTLCWGFADTPFGRACLTLAPRGLHRLAFA
                     DTADAELARLRADWPHARLVE"
BASE COUNT          517 a         1265 c         1273 g          465 t
ORIGIN      
        1 gaaaaacgct cgcggcggcc agcaggagag ccgccgcggg cgcgaggcgg gagtagcggc
       61 ggttggagcg ggggaagtcc acgcccatgg ccacgccaaa gagcagggcc atgccgagtg
      121 cggcgagcag cacggcgctt ccccctgtca agcgtcggat catcgtcgtg acttcacacc
      181 agaaaccggt tcggccgcat gttaacggcg cccgccgacc cggcacagcc gctgccaccg
      241 gaagatacag cgcaagcggg gttcactgac cacccgacac cgcggccggc ggtcgcagcg
      301 tcagcgtgcc gagcgcgccg gcctcgtagt cgcgcgcctg catcgacatc ggcagtgatt
      361 cgacgcgcgt ccagcgatcg agcagatcgg cgtagtgcgg cgacagcgga ttgccgctct
      421 ggccgctcga atgcatgaag gtcgaacgat ccggatcggc aaggtcgacg atcatgcgca
      481 gactggcgac gtggcgggcg gcgtacggcg cctcgacatc attgatgaag aagcgcgcga
      541 catcgagcgt cgagacatcg ccgccgaccg gccggcgcag ctcgaacagc ggccgcagcc
      601 aggcgagctt cgagaacggc cggtgctcgg cgacggccgg atgcgcgtcg ccccagtgcc
      661 agcgcgattc atcgcttccg aagcgcgcgt gcagctcgtc gagcgcctcg ccgagcgccg
      721 cgaccacctg ctgcgcgcag gactcggtcg cggccgtgcg cacgtcatca caccagagcc
      781 cctgcccgtc gcgatcgtcg agcacgttgt tcatgaaggt cgtgcgctgg tcccacagcc
      841 gctcgaactc gggcccgagc tcgtcggcgt agatgcgccg ggtcagcgcc cggtaccagg
      901 ccgaaaacac cagcggctcg gcggcatcga cgcgcatgcc gtaatcccag cgcgccagcc
      961 gctcgtgcca gccgcgcgcc gccggcgggt agtcggtggc cgctgcgcgc tgcagcagct
     1021 tcggcagcaa atcggcggcg agccccgagc ggatgtcggc gtgcatctgc cggaagctcg
     1081 ccggcgtgtg cgccggcgtc gcggccagca gctcccggat gcgcgcggcg cggtaggccg
     1141 gctgccattc gtcggtcagg tgccagcggt agccgggcgg tacgatgcgt tcgttggcgc
     1201 tgtagagcgc gccgtcggcc ggatcgaaca cgcgcggcaa ttcctcgtac ggcacccagc
     1261 cggcccagtc gtagcgcgcc tcccagcccg gcgccggcgc cagcccgagc aggtcgttgt
     1321 cgggcctgcg caccgggatg cgccccggcg acaggaagcc gatgtgaccg tcgacatcgg
     1381 cgtacacgaa gttctgctgc ggcgcgacga actcgcgcag cgcctcgttg aagccggtcc
     1441 agtcctgcgc gtacgcgagc ttcgaggcgg cccggaagct cgcgtcgtcc tcggcgagcg
     1501 ccgtccacgc cagcgccagc ccgtagccgg ccggcagcgc atcgccggtg cgggtgtccg
     1561 gcagcagcgg gccgtggcgg ctggtgcgca cgatcagccg ttcgtcgggc gcgttgcgca
     1621 cgtgcagaat ttcctcgcgc tgttcgaaac gctgccagcc gtccggcgtc tggtaacgcc
     1681 ccggatcggc cggatcgctg cgttcgacat aaagatcctg cacgtccggg ttggtgttgg
     1741 tcaggcccca ggcgatgcgc tcgttgcggc cgatcacgac catgggcacg ccgggcagcg
     1801 tcgcgccgat cacgcgcagg cccggggctt cgaggtgcgc gaagtaccag atcgccggcg
     1861 ccgtcagccc gaggtgcgga tcgttggcga gcagcggctt gccgctggca ctgcggctgc
     1921 cggcgaccac ccagttgttc gagcccaggc catcggtatc cgtgcggggt tgcggaccga
     1981 gggcggcgag cggcaggtcg agcccgcggt acagcgccgc gtagtcggcg accggcagcg
     2041 gtccggcgta cggcaccggc ggcaggaagt cctggatgcg cgcggtcggc aattgtctgg
     2101 cgagccgcag gcgcgtcagc tcgccgctcc agttgctgcc gagatcgagc gccatgacct
     2161 tcagccagga cagcgaatcg gccggctgcc aggcctcggg gcgcacgccg gtcagcagga
     2221 attcgggcgg cagcggcccg ccgtgcgcgg caatccaggc gttgacgccg gcggcgtagg
     2281 catcgagcac ggcgcgggat tcggcatcga ggttgcggta attgcgctcg gcgacgcgcc
     2341 gaagccccat cgtgcgcagc agccggtcgg tgtcgagcgc cgtcgcaccg agcgcttcgg
     2401 cgatgcgccc gccggcgatg cgccgcgaca tctccatctg ccacagccgg tcctgcgcat
     2461 gcacgacgcc gagggcgaac caggcatcgt ggacattctg cgcacggatg tgcggcacgg
     2521 ccgcggcgtc gcgcacgatg ctcaccggcg cctgcaggcc gggcaggctc agctcgccgg
     2581 cgagcggcgg cagcgagccg cgcagccagt accagccgcc ggcgatcccg agtgcgagca
     2641 gcgccgtcag caccgccagc gtccgccccg ccgtgcgcgc gaatcgatgc gacatcccca
     2701 tgaccgatcc cctcttccgg ccgctgccgc ggcgaaagtc cgcgatgatg cacatccggc
     2761 cggacgcgcg tcaccgcagc ttcatgacag tttcatcacc ccgtcatgtt cctgcgctgc
     2821 gacgagccac atggaggcag ccaccggccg ggcgtgcaca aaaaatcccg gtgcagcctc
     2881 cggaccggct gcgggcgaag ccgcaggaat cgggtgggcg gaccgccggg cagcggccag
     2941 aatgccgccc gcccgtcccc gtccgcggag agcccgcatg tccccgtctg ccgaaaccgc
     3001 tgccgccgtc ccgtccaccg gaatcttcga agccgtcgaa cccgcgcgcg catggacgat
     3061 ggtcgcgggc gcgatcgagc agctctgcgc gcggagcccg gccgcggtgc cgctcgccga
     3121 gctggcggcg gcggccggcc tgagcgaagg ccagttccag cgcgtgttct cggcctgggc
     3181 cggcatcagc ccgaagcgct tccagcagtt tctcggcaag gaatacgcga agcaggcgct
     3241 gcgcgccggg caggacgtgc tgaacgccgc gctcgccgcc ggcctgtcgg gtccgggccg
     3301 gctgcacgat ctgctggtca gcgccgaagc cgtgtcgccc ggcgaatggc gcgccggcgg
     3361 cagcggcctg acgctctgct ggggtttcgc cgacacgccg ttcggccgcg cctgcctgac
     3421 gctcgcgccg cgcggcctgc accggctggc gttcgcggac acggccgatg cggaacttgc
     3481 gcgcctgcgt gccgactggc cgcacgcacg gctggtcgag
//
LOCUS       DAOQJH010000249         3518 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_16947_length_3518_cov_3.863413, whole genome shotgun sequence.
ACCESSION   DAOQJH010000249 DAOQJH010000000
VERSION     DAOQJH010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3518
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16947_length_3518_cov_3.863413"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..651)
                     /locus_tag="PLQ18_06745"
     CDS             complement(<1..651)
                     /locus_tag="PLQ18_06745"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNE01389.1"
                     /translation="MFLGSAAAPLELEVISRLPAQAGDKPPLLFVHGAFAGAWCWEEH
                     FLDFFAGLGYPSYALSLRGHAGSAGRDRLHWNRLADYVTDVAEVAVGLGERTILIGHS
                     MGGMVVQKYLERTPAPAAVLMCSVPPSGLMWSSLQLMWRDPMLWMEMGVITHAGPQYG
                     SRSLARRALFSPDAPDAVVDRLMNRAQEESSRVLADLTWADLPAVTLVRRTPLLVLG"
     gene            complement(854..2089)
                     /gene="serA"
                     /locus_tag="PLQ18_06750"
     CDS             complement(854..2089)
                     /gene="serA"
                     /locus_tag="PLQ18_06750"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005672765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HNE01390.1"
                     /translation="MGKVSLDRREIRILLLEGIAPSAVETFRAAGYTNLEQHRKALPE
                     PELIERLREAYIVGIRSRTQLTPAVLAEAKKLIAVGCFCIGTNQVALEAAASAGVPVF
                     NAPFSNTRSVAELVMAEIVLLMRGIPAKNALCHRGGWSKSAAGSREVRGKRLGIVGYG
                     HIGTQVGILAEAFGMQVHYYDIEAKLALGGAQPVHSLAELLARSDVLTLHVPETPQTR
                     NMIGAAELARLPAGAQLINASRGTVVDIDALAAALASGHVAGAAIDVFPAEPQGNDDE
                     FVSPLRAFDQVLLTPHIGGSTEEAQENIGFEVAGKLVRYSDNGSTLSAVNFPEVSLPA
                     HPGKRRLLHIHENRPGLLSAINDALSRQRINIAGQYLQTRGGIGYVVIDIDDDEHAGK
                     LALKELRAIDGTLRTRILY"
     gene            2234..>3518
                     /locus_tag="PLQ18_06755"
     CDS             2234..>3518
                     /locus_tag="PLQ18_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017463623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNE01391.1"
                     /translation="MSDAAILARLGALLPPARLRTDADTLASHGRDWTRLYQPDPLAV
                     AFPESVAEVQALVAIANDTACPLVPSGGRTGLSGGAVAKSRELVLSFERMNRILGFDA
                     VDRSVTAEPGVILETLQDYAREHGLQYPVDFAARGSAQLGGTIATNAGGIKVIRWGMT
                     RDWVSGLKVVTGRGELLDLNRGLVKNNTGYDLRQLFIGSEGTLGLIVEATVRLAEAPQ
                     APAVMVLGLPDLEAIMSAFERLRAALRLTAFECFSAQALSYVTAHGQPRPFETETPWY
                     VLAEYDAAEERDTEAAMAAFETLAEDGIVLDGVLSQSDSQWRALWRLREDITESIAHR
                     LPYKNDVSVRVARVPEFLRAMDELFAREYPDFEVIWFGHVGDGNLHISVLQPPEMARA
                     DFVAACEQVSEHLFATLAALGGSISAEHGVGLVKKP"
BASE COUNT          538 a         1233 c         1242 g          505 t
ORIGIN      
        1 gccgagcacc agcagcggcg tgcgccgcac cagcgtcacc gccggcaggt cagcccaggt
       61 caggtcggcg agcacgcgcg aggattcctc ctgcgcgcgg ttcatcagcc ggtcgacgac
      121 ggcatcgggt gcatcgggcg agaacagcgc gcgccgggcg agcgagcggc tgccgtactg
      181 cggaccggcg tgcgtgatga cgcccatctc catccacagc atcggatcgc gccacatcag
      241 ttgcaggctc gaccacatca gccccgaggg cggcaccgag cacatcagca ccgccgccgg
      301 cgccggcgtg cgctccagat acttctgcac gaccatgccg cccatcgaat gaccgatcag
      361 gatcgtgcgc tcgccgaggc cgacggcgac ctcggcgaca tcggtcacgt agtcggcgag
      421 ccggttccag tgcaggcggt cacgcccggc gctgccggca tggccgcgca gcgacagcgc
      481 ataggacggg tagccgagac cggcgaagaa atcgaggaag tgttcctccc agcaccaggc
      541 accggcgaag gcgccgtgca cgaacagcag ggggggcttg tcgccggcct gcgcgggcag
      601 gcggctgatg acttccagtt cgagaggcgc ggcggcgctg cctagaaaca cggtgcgtaa
      661 cctcgggcgc ggatggggga atcggtcgga cagacgcgca gacagcaagg cagcgcgcgg
      721 gcatgcgcag gcgcaggccg cgtccggaca atcggtaacg cagacggcgg gcggctgcac
      781 gtcaacatcg gtcaggacac cgggatttgc gcggacggcg acgagggagc gtcggggcag
      841 agccggcggg gcttcagtac aggatgcgcg tgcgcagcgt gccgtcgatc gcgcgcagtt
      901 ccttcagcgc cagcttgccg gcgtgctcgt cgtcgtcgat gtcgatgacc acgtagccga
      961 tgccgccgcg ggtctgcagg tactggccgg cgatgttgat gcgctggcgt gacagcgcat
     1021 cgttgatcgc cgacagcagg ccggggcggt tctcgtggat gtgcagcagc cggcgcttgc
     1081 cggggtgtgc gggcaggctg acctcgggga agttcacggc cgacagcgtc gagccgttgt
     1141 cgctgtagcg caccagcttg ccggcgacct cgaagccgat gttctcctgc gcctcctcgg
     1201 tgctgccgcc gatgtgcggg gtcagcagca cctgatcgaa ggcgcgcagc ggcgacacga
     1261 actcgtcgtc gttgccctgc ggctcggccg ggaacacgtc gatcgccgcc ccggcgacat
     1321 ggccgctcgc gagcgccgcc gccagcgcgt cgatgtcgac gacggtgccg cgcgaggcat
     1381 tgatcagctg cgcgccggcc ggcagccgcg ccagttcggc cgccccgatc atgttgcggg
     1441 tctgcggcgt ctcgggcacg tgcagcgtca gcacgtcgga gcgcgcgagc agctcggcaa
     1501 gcgaatgcac cggctgcgcg ccgccgagcg cgagcttggc ctcgatgtcg tagtagtgca
     1561 cctgcatgcc gaaggcctcg gcgaggatgc cgacctgcgt gccgatgtgg ccgtagccga
     1621 cgatgccgag gcgcttgccg cgcacctcgc gactgccggc ggccgacttc gaccagccgc
     1681 cgcggtggca gagcgcgttc ttggccggga tgccgcgcat cagcagcacg atctcggcca
     1741 tcaccagctc ggcgacgctg cgcgtgttcg agaacggcgc gttgaacacc ggcacgccgg
     1801 cgctcgcagc ggcctcgagg gcgacctggt tggtgccgat gcagaaacag ccgacggcga
     1861 tgagtttctt cgcctcggcg agcacggccg gagtcagctg cgtgcgcgaa cggatgccga
     1921 cgatgtaggc ctcgcgcagg cgctcgatca gctcgggttc gggcagcgcc ttgcggtgct
     1981 gttcgaggtt ggtgtaaccg gcggcgcgga aggtctcgac cgcactcggc gcgatgcctt
     2041 cgagcagcag aatcctgatt tcgcgtcggt cgagggaaac cttgcccatg gtcggatgct
     2101 cctcggattg ccggcagacg cggccgggcc ggcgatgatg tcgcaaaatg ccggtggccg
     2161 gaaggccggg cagcagcccg gccctcgcct acgctgcacc agcgcagcct ctgtaaccct
     2221 gccggagacc gccatgtccg atgccgccat cctcgcccgc ctcggcgcgc tgctgccacc
     2281 cgcgcgcctg cgcaccgatg ccgacaccct cgccagccac ggccgcgact ggacccgcct
     2341 gtaccagccc gacccgctcg cggtcgcctt ccccgaatcg gtcgccgaag tgcaggccct
     2401 cgtcgcgatc gccaacgaca ccgcctgccc gctcgtgcct tcgggggggc gcaccggcct
     2461 ctccggcggc gcggtggcga agtcgcgcga gctggtgctg tccttcgagc gcatgaaccg
     2521 catcctcggc ttcgatgcgg tcgatcgcag cgtcaccgcc gaacccggcg tcatcctcga
     2581 aaccctgcag gactacgccc gcgagcatgg cctgcagtat ccggtcgact tcgcggcgcg
     2641 cggctcggcg cagctcggcg gcacgatcgc gaccaacgcc ggcggcatca aggtgatccg
     2701 ctggggcatg acccgtgact gggtcagcgg cctgaaggtc gtcaccggcc gcggcgagct
     2761 gctcgatctg aaccgcggcc tggtcaagaa caacaccggc tacgacctgc gccagctgtt
     2821 catcggctcc gaaggcacgc tcggcctgat cgtcgaggcc acggtgcgcc tcgccgaagc
     2881 gccgcaggca ccggcggtca tggtgctcgg cctgcccgac ctcgaagcga tcatgagcgc
     2941 cttcgagcgc ctgcgcgccg cgctgcgcct gacggccttc gaatgcttct cggcgcaggc
     3001 gctttcctac gtcaccgccc acggccagcc gcggccgttc gagaccgaaa cgccgtggta
     3061 cgtgctcgcc gagtacgacg ccgccgaaga gcgcgacacc gaagccgcga tggcggcctt
     3121 cgagacgctc gccgaggacg gcatcgtgct cgatggcgtg ctgtcgcaga gcgacagcca
     3181 gtggcgggcg ctgtggcgcc tgcgcgagga catcaccgag tcgatcgcgc accgcctgcc
     3241 gtacaagaac gacgtctcgg tgcgcgtcgc gcgcgtgccg gaattcctgc gcgcgatgga
     3301 tgaactcttc gcgcgcgaat atccggactt cgaggtgatc tggttcggcc acgtcggcga
     3361 cggcaacctg cacatctcgg tgctgcagcc gccggaaatg gcacgcgccg acttcgtcgc
     3421 cgcctgcgag caggtcagcg agcacctgtt cgcgacgctc gccgcgctcg gcggcagcat
     3481 ctcggccgag cacggcgtcg ggctggtcaa gaagccct
//
LOCUS       DAOQJH010000250         3508 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_17035_length_3508_cov_3.377932, whole genome shotgun sequence.
ACCESSION   DAOQJH010000250 DAOQJH010000000
VERSION     DAOQJH010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3508)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3508)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3508
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17035_length_3508_cov_3.377932"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..878)
                     /gene="argH"
                     /locus_tag="PLQ18_06760"
     CDS             complement(<1..878)
                     /gene="argH"
                     /locus_tag="PLQ18_06760"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015488761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HNE01392.1"
                     /translation="MSQTNQQWGGRFTESTDAFVQAFTASVQFDQRMYRQDIAGSIAH
                     ATMLAEAGVLNEADRAAIVAGLEAIRAEIERGEFEWSIALEDVHMNVEARLTERIGLA
                     GKRLHTGRSRNDQVATDIRLWLRDAIDAILAELARLQHGLVELAEREADTIMPGFTHL
                     QVAQPVSFGHHLLAWFEQLKRDSGRYRDCRARLNECPLGAAALAGTTYPIDRHRTASL
                     LGFAGPTENSLDSVSDRDFAIEFCSASALLMTHLSRFSEELVLWASAQFGFVDIPDRF
                     CTGSSIMPQKKNPDVP"
     gene            complement(967..2046)
                     /locus_tag="PLQ18_06765"
     CDS             complement(967..2046)
                     /locus_tag="PLQ18_06765"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNE01393.1"
                     /translation="MSWSLSSAHTDFPAFAPAWDALNRRLFNAHPLLDSAFIAPLIEL
                     YGPDTLRLARETVDGQLRSAALIRPLAPGAWRVFALGPIQMTPALMAPEADWDELLRS
                     LPGMALLLNVNLQDPQYSHLAPCPPVRRAFDHACTIGIALEGDFDRYWQGRSRHLRQN
                     LGRYQRRLREDGMAVEWRQYAEPDAIVAAVDRYSVLESAGWKGRNGTALHPDNLQGEF
                     FRRVMQRFAHRASARVWELWIDGQLAASRLAIGTPDMLVMLKTTYDEQLARYAPGMLL
                     LRQVIEEEFTAARHRRIEFYTNATPAQHAWATDSRWIRHFEVYRNRLSASVIDGVRLS
                     RAHPRALLGGTAAVAALAAFSALLN"
     gene            complement(2162..2617)
                     /locus_tag="PLQ18_06770"
     CDS             complement(2162..2617)
                     /locus_tag="PLQ18_06770"
                     /inference="COORDINATES: protein motif:HMM:NF021158.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HNE01394.1"
                     /translation="MTVPRHPARQIALAFACSVVLLAACTGPTGSERATVTSDAYRHA
                     RPGELTGSVSWLQRLLPPPGAGLTVELIEAGPRARVLAEQRLARLGAPPWRFRLEFDP
                     ARIDPAQRYVLRARVDFDGRTRFANRLDYPVLTHDAPAHVDVIVDAALP"
     gene            complement(2633..>3508)
                     /locus_tag="PLQ18_06775"
     CDS             complement(2633..>3508)
                     /locus_tag="PLQ18_06775"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A biosynthesis acyltransferase"
                     /protein_id="HNE01395.1"
                     /translation="AWLLQCLPQRARDALARLVLPLVWRLTGRQQHNARINLGLCFPQ
                     QPPEWREQVLREHIALAVRVTLDYGLLWFGSATAIERAVRFHGEEHLAAARAEGRRVI
                     LLVAHALALDFGICAFGIRHRALGPYKALADPVIDWLIARARTRFEGSVFEREAGMRP
                     VIRGFRDGQILVYLADEDLGPDGAVFVPFFAEPKATLATLGRLAKLGDARVIPSVTRW
                     NAAEGVYDCFHRPPLADFPSGDAERDARAMNAAVEALITPEPAQYMWTLRLFRTRPSG
                     RAKLYKQRKTPPAGG"
BASE COUNT          524 a         1145 c         1327 g          512 t
ORIGIN      
        1 tccggcacgt cggggttctt cttctgcggc atgatcgagc tgccggtgca gaagcgatcc
       61 ggaatgtcga cgaagccgaa ctgcgcgctc gcccacagca ccagctcctc gctgaagcgc
      121 gacaggtggg tcatcagcag cgccgaggcc gagcagaatt cgatcgcgaa gtcgcggtcg
      181 ctgaccgaat cgagcgagtt ctcggtcggt ccggcgaagc cgagcaggct ggcggtgcgg
      241 tggcggtcga tcggataggt ggtgccggcg agtgccgcgg cgccgagcgg gcattcgttc
      301 aggcgtgcgc ggcagtcgcg gtaacggccg ctgtcgcgct tgagctgttc gaaccacgca
      361 agcaggtggt ggccgaagct gaccggctgc gcgacctgca ggtgcgtgaa gccgggcatg
      421 atggtgtcgg cctcgcgctc ggcgagttcg accagcccgt gctgcaggcg cgcgagttcg
      481 gcgaggatcg cgtcgatggc gtcgcgcagc cacaggcgga tgtcggtggc gacctgatcg
      541 ttgcgcgagc ggccggtgtg caggcgcttg ccggcgaggc cgatgcgctc ggtcaggcgc
      601 gcctcgacgt tcatgtgcac atcttcgagc gcgatcgacc attcgaactc gccgcgctcg
      661 atctcggcgc ggatggcttc cagtccggcg acgatcgccg cgcggtcggc ctcgttcagc
      721 acgccggcct cggcgagcat ggtcgcgtgc gcgatcgagc cggcgatgtc ctggcggtac
      781 atgcgctggt cgaactgcac cgaggcggtg aaggcctgca cgaaggcgtc ggtggattcg
      841 gtgaagcgtc cgccccactg ctgattggtc tggctcatgg ctgcatcggt ccgccgccgc
      901 aggaaaaagg gcggcaagta tacggcgcga tccctgctgc ggaccgcgcg ccgatcgcgc
      961 cggggttcag ttgagcagtg ccgaaaacgc cgccagcgcg gccacggccg ccgtgccgcc
     1021 gagcagcgcg cgcgggtggg cgcggctcag gcgcacgccg tcgatgacgc tcgccgacag
     1081 gcggttgcgg tagacctcga agtgacggat ccagcggctg tcggtcgccc aggcgtgctg
     1141 cgccggcgtg gcgttggtat agaactcgat gcgccggtgc cgtgcggccg tgaattcctc
     1201 ctcgatgacc tggcgcagca gcagcatgcc cggtgcgtag cgggcgagtt gctcgtcata
     1261 cgtggtcttc agcatgacca gcatgtcggg cgtgccgatc gccagccggg aagcggcgag
     1321 ctgcccgtcg atccagagct cccagaccct ggcgctggca cggtgcgcga agcgctgcat
     1381 cacgcgccgg aagaactcgc cctgcaggtt gtccggatgc agcgccgtgc cgttgcggcc
     1441 tttccagccg gcgctttcca gcacgctgta gcgatcgacc gccgcgacga tcgcgtccgg
     1501 ctcggcgtac tggcgccatt cgacggccat gccgtcctcg cgcaaccggc gctggtagcg
     1561 gccgaggttc tgacgcaggt ggcgggagcg gccctgccag tagcggtcga aatcgccctc
     1621 cagtgcgatg ccgatggtgc aggcgtgatc gaaggcgcgg cgcaccggcg ggcagggggc
     1681 gagatgcgag tactgcgggt cctgcagatt gacgttcagc agcagcgcca tgccggggag
     1741 gctgcgcagc agttcgtccc agtcggcctc gggggccatc agcgccggag tcatctggat
     1801 cgggccgagc gcgaacacgc gccaggcacc gggggccagc ggccggatca gcgcggccga
     1861 gcgcagctgg ccatcgacgg tctcgcgggc gaggcgcagc gtgtccggac cgtacagctc
     1921 gatcagcggc gcgatgaatg cgctgtcgag cagcgggtgc gcgttgaaca gccgtcggtt
     1981 cagggcatcc catgccggtg cgaaggcagg gaaatccgta tgggcggagg aaagcgacca
     2041 ggacatgtcc aatctcggta ttgcgcgctt cctgcgccgg ctatcggggt cgggacacag
     2101 tccttcgctg tgtcaggccc ggcagcatag gcaaaaccac gttcagtaca agtgattccg
     2161 gtcatggcag cgctgcatcg acgatgacgt cgacgtgcgc cggggcatca tgagtcagca
     2221 ccgggtagtc gagacggttg gcaaagcggg tgcggccgtc gaagtcgacg cgggcgcgca
     2281 gcacgtaacg ctgcgccgga tcgatgcgtg ccgggtcgaa ttcgagccgg aagcgccacg
     2341 gcggtgcgcc gagtcgtgcc agccgctgct cggccagcac ccgggcccgg gggccggcct
     2401 cgatcagttc gaccgtcagt ccggcgccgg gcggcggcag caggcgctgc agccagctga
     2461 cgctgccggt cagttcgccg gggcgggcgt ggcggtaggc atcgctggtc acggtcgccc
     2521 gttcgctgcc ggtcgggccg gtgcaggcgg cgagcagcac gacgctgcag gcgaaggcga
     2581 gggcgatctg gcgggccgga tggcggggca cggtcatgat ggggcagggt cctcagccgc
     2641 cggcgggcgg ggtcttgcgc tgcttgtaga gcttggcgcg gccgctcggg cgcgtgcgga
     2701 acaggcgcag cgtccacatg tactgcgccg gctcgggggt gatcagcgcc tcgacggcgg
     2761 cgttcatcgc gcgcgcgtcg cgctcggcgt caccactcgg gaaatccgcg agcggcggcc
     2821 ggtggaagca gtcgtagacg ccttcggcgg cgttccagcg cgtcaccgac gggatcacgc
     2881 gcgcgtcccc gagcttcgcg agccgcccga gcgtcgcgag cgtcgccttc ggctcggcga
     2941 agaacggcac gaacaccgca ccgtcggggc cgaggtcctc gtcggccaga tacacgagga
     3001 tctgcccgtc ccgaaagccg cggatcaccg ggcgcatgcc ggcctcgcgt tcgaacaccg
     3061 aaccttcgaa gcgcgtgcgg gcgcgcgcga tcagccagtc gatgaccgga tcggccagcg
     3121 ccttgtacgg cccgagcgcg cggtggcgga tgccgaaggc gcagatgccg aaatcgagcg
     3181 ccagcgcgtg cgcgaccagc aggatcacgc gccggccttc ggcgcgggcc gcagcgaggt
     3241 gctcctcgcc gtggaagcgc acggcgcgct cgatcgccgt ggcgctgccg aaccacagca
     3301 ggccgtagtc gagcgtcacg cgcacggcca gcgcgatgtg ctcgcgcagc acctgctcgc
     3361 gccattcggg cggctgctgc ggaaagcaca ggccgagatt gatgcgggcg ttgtgctgct
     3421 gcctgccggt caggcgccag accagcggca gcacgagccg cgccagggcg tcacgcgcgc
     3481 gctgcggtag gcattgcagc agccaggc
//
LOCUS       DAOQJH010000251         3505 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_17057_length_3505_cov_4.669855, whole genome shotgun sequence.
ACCESSION   DAOQJH010000251 DAOQJH010000000
VERSION     DAOQJH010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3505
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17057_length_3505_cov_4.669855"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..474
                     /locus_tag="PLQ18_06780"
     CDS             <1..474
                     /locus_tag="PLQ18_06780"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNE01396.1"
                     /translation="ITSIAEQTNLLALNATIEAARAGEAGKGFAVVAGEVKELARATA
                     EATGRIHERIDNIQRDTAQAQTAIARTRDIIGRVNAFEQSIAQAAEQQSLAAAEMAGH
                     VAEAAQRGQHIAQVVRELAAGADGTVHASAEVLDSARALSETAGTLTERVGQFRT"
     gene            552..698
                     /locus_tag="PLQ18_06785"
     CDS             552..698
                     /locus_tag="PLQ18_06785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE01397.1"
                     /translation="MRAYIGQGLRADLERLDAEPVSRLLAALRAEGVPEETLAKAVEA
                     LTTA"
     gene            complement(712..2286)
                     /gene="gshA"
                     /locus_tag="PLQ18_06790"
     CDS             complement(712..2286)
                     /gene="gshA"
                     /locus_tag="PLQ18_06790"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HNE01398.1"
                     /translation="MYRSAQARLTRLLDAAGPVAVRGCLKGLEREALRVAPDGRIAQT
                     RHPRALGAALTHPWITTDYSEALLEFITPPVADTQDCTRFLDEVHRFVLPRLGDEKIW
                     PGSMPCVVDGEDGIPIAEYGSSNAGYMKHVYRRGLGYRYGKLMQVIAGVHFNFSLPEA
                     FWAALREIDGSIAAQPAYVSDGYFRLTRNVQRVGWLVPYLFGASPAVCRSFIGARGED
                     LDEWDDGTLYLPYATSLRMSGIGYQNRKEKKCGVHVRYDGLDAYVASLARAVNTPCPE
                     YEAIGVCRAGDWRQLSGNILQIENEYYSTVRPKQPPLGNEKVTAALARRGVRYVELRS
                     LDVDPYQPTGVDAVTLRFVEALLIFCTLADSPRIELQEQLDVNANLERVARAGRDPGL
                     LLARRGASIGLRDWGLGILAALEPVCAVLDGADPESPYTAALNEQRGKLEDPARTPSA
                     RVLAEMGEREEGFFDFGWRLATEHAATLRRRPLRPAREAEFRVLAERSHAEQAAMEAA
                     DDIGFEEYLRRYFAQR"
     gene            2587..>3505
                     /locus_tag="PLQ18_06795"
     CDS             2587..>3505
                     /locus_tag="PLQ18_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006081592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde 3-phosphate dehydrogenase
                     NAD-binding domain-containing protein"
                     /protein_id="HNE01399.1"
                     /translation="MSQAQHDLILQDWKNRQEIAEEMLPLLGRLYRDRGLVLLVYGRP
                     IVNSSTIEILKAHRYVRYHEGAELDVADTLAVLKAIAGMDVAPARLDIGKLAVGFRKA
                     GTAVSLADYLGEQLADIVGDHAQKSLEPKDVVLFGFGRIGRLLARLLIERTGAGNKLR
                     LRAIVVRKGKGADLQKRASLLRRDSVHGPFNGSITVDEENNAIIANGNFIKVIYADKP
                     GAVDLTEYEINDALIVDNTGKWADLEGLTQHLSGKGAKKVLLTAPSKGDGIKNIVYGV
                     NHGTIEDSDLVVSAASCTTNAITPVRISVY"
BASE COUNT          593 a         1259 c         1169 g          484 t
ORIGIN      
        1 atcacgtcga tcgccgagca gaccaatctg ctggcgctga acgccaccat cgaggccgca
       61 cgcgccggcg aggccggcaa gggtttcgcc gtggtcgccg gcgaggtcaa ggaactggcg
      121 agagccaccg ccgaggccac cggccgcatc cacgagcgca tcgacaacat ccagcgcgac
      181 accgcgcagg cccagaccgc catcgcccgc acccgcgaca tcatcggtcg cgtcaacgcc
      241 ttcgagcagt cgatcgcgca ggccgccgaa cagcagtcgc tcgccgccgc cgagatggcc
      301 ggccacgtcg ccgaggctgc gcagcgcggc cagcacatcg cccaggtcgt gcgcgaactc
      361 gccgccggcg ccgacggcac ggtgcacgcc agcgccgagg tcctcgactc cgcccgcgca
      421 ctgagcgaaa ccgccggcac gctgaccgag cgggtcggac agttcaggac ctgagcgggc
      481 gctgcaagag gtgggcggat ggcggaagcg catcgccccc cctgcccggc cattccggct
      541 accagcccct gatgcgcgcc tatatcgggc agggcttgcg cgccgatctg gagcggctcg
      601 atgccgaacc ggtcagccgg ctgctggcag cgctgcgggc cgagggcgtt cccgaggaaa
      661 cgctggcgaa agcggtcgag gccctgacga ccgcctgaac cgccggccgc ctcagcgctg
      721 cgcgaaatac cggcgcaggt attcctcgaa gccgatgtcg tcggcggcct ccatcgccgc
      781 ctgctcggcg tgcgagcgct cggcgagcac gcggaactcg gcctcgcgcg ccggccgcag
      841 cggccggcgg cgcagcgtcg cggcgtgttc ggtggccagg cgccagccga agtcgaagaa
      901 accctcctcg cgctcgccca tctccgcgag tacgcgcgcc gacggcgtgc gcgccgggtc
      961 ttcgagcttg ccgcgctgct cgttcagcgc cgccgtgtac ggcgattccg gatcggcgcc
     1021 gtcgagcacc gcacacaccg gttccagcgc cgcaaggatg ccgagccccc agtcgcgcag
     1081 gccgatgctg gcgccgcggc gcgccagcag cagaccggga tcacgcccgg cgcgtgccac
     1141 gcgttcgagg ttggcgttca cgtcgagctg ctcctgcagc tcgatgcgcg gactgtcggc
     1201 cagcgtgcag aagatcagca gcgcctcgac gaagcgcagc gtcaccgcat cgacgccggt
     1261 cggctggtac ggatcgacat cgagcgagcg cagctcgacg tagcgcacgc cgcggcgcgc
     1321 cagcgccgcc gtgaccttct cgttgccgag cggcggctgc ttgggccgca cggtgctgta
     1381 gtactcgttc tcgatctgca ggatgttgcc gctcaactgc cgccagtcgc cggcccggca
     1441 gaccccgatc gcctcgtact cgggacaggg cgtgttgacg gcgcgcgcga ggctcgcgac
     1501 gtaggcatcg aggccgtcgt agcgcacgtg cacgccgcac ttcttctcct tgcggttctg
     1561 gtagccgatg ccgctcatgc gcagcgaggt cgcgtacggc aggtacagcg tgccgtcgtc
     1621 ccattcgtcg agatcctcgc cgcgcgcgcc gatgaacgag cggcacacgg ccggcgaagc
     1681 gccgaacagg tagggcacca gccagccgac acgctgcacg ttgcgcgtca gccggaagta
     1741 gccgtccgac acataggccg gctgcgcggc gatgctgccg tcgatctcgc gcagcgccgc
     1801 ccagaaggcc tccggcagcg agaagttgaa atgcacgccg gcgatcacct gcatcagctt
     1861 gccgtagcgg tagccgagcc cgcggcggta gacgtgcttc atgtagccgg cattggagct
     1921 gccgtactcg gcgatcggga tgccgtcctc gccatcgacg acgcagggca tgctgcccgg
     1981 ccagatcttc tcgtcgccga ggcgcggcag cacgaagcga tgcacctcgt cgagaaagcg
     2041 cgtgcagtcc tgcgtatcgg cgaccggcgg cgtgatgaac tccagcagcg cctcggaata
     2101 gtcggtcgtg atccacggat gcgtcagcgc cgcgccgagc gcgcgcgggt gccgggtctg
     2161 cgcgatgcgg ccatccggcg caacgcgcag cgcctcgcgt tcgagcccct tcaggcagcc
     2221 gcgcacggcc accggaccgg cggcatcgag gaggcgggtc agacgggctt gggcggatcg
     2281 gtacacggag ggatcactcg ctgggggcac ggaacgcggc ggtccgggcc ggtcatccgc
     2341 gtcaggacgc gcatgatagc gcgcacatcc gcagcacccc gccgccggcg tgcatcgccg
     2401 ctctgcagcc agcacgccgc cgacacgcag cttgaccgtg aggacgcggt ttggttgacc
     2461 tgcgtgcttg ccgtgcaggg agcccgatca ttacaatccg cgcccgcttg cgactgtttc
     2521 tgcaggattt ttcgacatgc ggaacgatcg agcgagcagc aacccatgcc caacggaaag
     2581 cgaacaatga gccaagcaca gcacgatctg attctgcagg actggaagaa ccgtcaggaa
     2641 atcgccgaag agatgctgcc gctgctcggc cggctctatc gcgaccgcgg cctggtcctg
     2701 ctggtctacg gtcgtccgat cgtcaacagc tccacgatcg aaatcctcaa ggcccaccgc
     2761 tacgtccgct accacgaagg cgccgagctc gatgtcgccg acacgctggc cgtgctgaag
     2821 gccatcgccg gcatggacgt ggccccggcc cgcctcgaca tcggcaagct cgccgtcggc
     2881 ttccgcaagg ccggcaccgc tgtctcgctc gcggactacc tcggcgaaca gctcgccgac
     2941 atcgtcggcg atcacgcgca gaagtcgctg gaaccgaagg atgtcgtgct gttcggcttc
     3001 ggccgcatcg gccgcctgct ggcccgcctg ctgatcgagc gcaccggcgc cggcaacaag
     3061 ctgcgcctgc gcgcgatcgt cgtgcgcaag ggcaagggcg ccgacctgca gaagcgcgcc
     3121 agcctgctgc gtcgcgactc cgtgcacggc ccgttcaacg gctcgatcac ggtcgatgaa
     3181 gaaaacaacg ccatcatcgc caacggcaac ttcatcaagg tcatctatgc cgacaagccc
     3241 ggtgccgtcg acctgaccga atacgagatc aacgatgcgc tgatcgtcga caacaccggc
     3301 aagtgggccg acctcgaagg cctgacccag cacctgagcg gcaagggcgc gaagaaggtc
     3361 ctgctgaccg ctccgtccaa gggcgacggc atcaagaaca tcgtctacgg cgtcaaccac
     3421 ggcacgatcg aggacagcga cctggtcgtc tcggccgcct cgtgcacgac caacgccatc
     3481 accccggtgc ggatctcggt gtaca
//
LOCUS       DAOQJH010000252         3503 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_17083_length_3503_cov_3.468097, whole genome shotgun sequence.
ACCESSION   DAOQJH010000252 DAOQJH010000000
VERSION     DAOQJH010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3503)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3503)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3503
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17083_length_3503_cov_3.468097"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(56..598)
                     /locus_tag="PLQ18_06800"
     CDS             complement(56..598)
                     /locus_tag="PLQ18_06800"
                     /inference="COORDINATES: protein motif:HMM:NF017415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HNE01400.1"
                     /translation="MATAKSTFGKEQLQDLLEKTKSSSHEVLLAGLGALSRAGKGEAN
                     GKKKRRDDFAALVEEGRKIEPKLKASMQKAWSGLKEKGSKMPSPGFESGKLQGVFEER
                     VSAALARMGIPTRKEIKALNQKVDRLIAAAQGRPAAKKAAPAKKSAAAGKSAAAKKSG
                     ARKTAAAKKSAAPAAAPSAE"
     gene            complement(680..1081)
                     /locus_tag="PLQ18_06805"
     CDS             complement(680..1081)
                     /locus_tag="PLQ18_06805"
                     /inference="COORDINATES: protein motif:HMM:NF017415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HNE01401.1"
                     /translation="MKSKRNAPEAAPFMTALSEGIEKGKDSVHDLLLAGLGALGRAQK
                     ARKERVAGLVAEGRKVEPRVKRAIQDWRAKMRLDGKSGLRAPAWLDGDKVRRLASDRI
                     ESALRHTGLPLRKDVEALSRKLDKLIELQRT"
     gene            complement(1209..1889)
                     /locus_tag="PLQ18_06810"
     CDS             complement(1209..1889)
                     /locus_tag="PLQ18_06810"
                     /inference="COORDINATES: protein motif:HMM:NF025338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNE01402.1"
                     /translation="MAARPARRPARPNPTRERILEASWRLFNELGVPNVTTNHIADEV
                     DISPGNLYYHFRHKEDIVNALYDRYEQRFSDVLGYSADELSTPDDLWLALHLAFEIIY
                     AYRFIYRDLSDLTTAYPGLRLRFQRSLEASIGKTREYLQRMARSGVIDALPEEISALA
                     ANMALVTAYWVNLHALRQPSRAVSRASDEQVVAQGVFQVLSLVTPHLRGPARESFRAH
                     ALRYAQRN"
     gene            complement(1905..>3503)
                     /locus_tag="PLQ18_06815"
     CDS             complement(1905..>3503)
                     /locus_tag="PLQ18_06815"
                     /inference="COORDINATES: protein motif:HMM:TIGR02946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HNE01403.1"
                     /translation="LVTAEPPKESQTDWLKLPTQRRRKPKPQRGWETLTDVDTADHVF
                     LHALPRPGGQRELGELVSRLHVARLDRSRPLWEVHLIEGLEGGRYAMYTKLHHSQFDG
                     KRGMDLIRYIRSSKASARNLPPLWAVKPPASEAGAGGPAASESTLRKIVDWGKALRAM
                     SSVRQNVPGDGVIAPYSAPESILNQPLTERRRLGTVSLPIDRLKALGAAIEGGATINE
                     VFLAICGGALRRYLAELKALPDKPLIAACPVAVARKDDTTAGNAVAQILVSLGTNIAN
                     PKKRLLAVVESSRVNKGQMKELGTEAYGIYTELSMIPQMLAAKTSFGHRVLNANLIIS
                     NMPGPRERQYVNGAELESMYAASLLLAGQALNITASSYHDSLDVGIIACPEVCPGPQK
                     IAVYAGEELAVLERAMQVGAKQAAGKTTKRAATKRAAKKPPARKTAAKKPAATKPAAK
                     KPAATKPAATKPAATKPAAKKPAARKPAAEKPVAKKTAAKKTGARKAPAAAAPASVAA
                     SAPDQAVATQPAKKVSARRAAGQG"
BASE COUNT          433 a         1228 c         1179 g          663 t
ORIGIN      
        1 ttcaccttca ttagtccaat atacccaccg ttcacgcgct gcgccggcat gacgatcact
       61 cagcgcttgg cgccgcggcc ggtgccgcgg acttcttcgc ggccgcggtc ttcctcgcgc
      121 cggacttctt cgctgcggcg gacttcccgg ccgcggccga cttcttcgcg ggggcggcct
      181 tcttcgccgc aggccggccc tgtgccgccg cgatcagccg atcgaccttc tggttgagcg
      241 ccttgatctc cttgcgcgtc gggatgccca tgcgcgccag cgccgccgag acgcgttcct
      301 cgaacacgcc ctgcagcttg ccgctctcga agcccgggct cggcatcttg ctgccctttt
      361 ccttcagccc gctccaggcc ttctgcatcg acgccttgag cttgggctcg atcttgcggc
      421 cctcttcgac gagtgccgcg aagtcgtcac ggcgcttctt cttgccgttg gcttcgccct
      481 tgccggcgcg cgacaaggcg cccaggccgg ccagcaggac ttcgtgcgac gaactcttcg
      541 tcttctcgag caggtcttgc aactgctcct tgccgaacgt ggatttcgcg gtagccatca
      601 tttctctccg gggggatccg aagccctctc gccgaaaagc gaggatacgc gcgcgcgagg
      661 gggcctcgat tgaatttcgt caggttcttt gcagttcgat cagcttgtcg agcttgcgcg
      721 acaaggcctc gacgtccttg cgcaagggca ggccggtatg gcgcaacgcg ctctcgatcc
      781 tgtcgctcgc caggcgccgc accttgtcgc cgtcgagcca ggccggcgcc cgcaggccgg
      841 acttgccgtc gagccgcatc ttcgcgcgcc agtcctggat cgcccgcttc acgcgcggct
      901 cgaccttcct gccctcggcg accagaccgg cgacgcgctc cttgcgcgcc ttctgggcgc
      961 ggccgagcgc acccagtccg gccagcagca ggtcgtgcac ggaatccttg cccttttcga
     1021 tgccctcgga aagggcggtc atgaacggcg ccgcttccgg cgcgttgcgc ttcgatttca
     1081 tctgcagctt cctcgctgtg agttgatccg ctgtcgatcc gatggcgccg ggcccgctgc
     1141 cgtgcaacgg cggccggggc aggtgtcgac ggcggaaatg ctatcggcgc tcgcctagag
     1201 cattcacctc aattgcgctg ggcgtagcgc agcgcgtggg cgcggaacga ctcgcgtgcc
     1261 gggccgcgca ggtgcggcgt gacgagcgac agcacctgga acacgccctg cgcgaccacc
     1321 tgctcgtcgc tcgcgcgcga gaccgccctc gacggctggc gcagcgcgtg caggttgacc
     1381 cagtaggcgg tgaccagcgc catgttcgcc gccagcgccg agatctcctc gggcagcgcg
     1441 tcgatcacgc ccgagcgcgc catgcgctgc aggtactcgc gggtcttgcc gatgctcgcc
     1501 tccaggctgc gctggaagcg caggcgcaga ccgggatagg cggtggtgag gtcgctcagg
     1561 tcgcgataga tgaagcggta cgcgtagatg atctcgaacg ccaggtgcaa cgccagccac
     1621 agatcgtccg gggtgctcag ctcgtcggcc gaatagccga gcacgtccga gaagcgctgc
     1681 tcgtagcggt cgtacagcgc gttgacgatg tcttccttgt ggcggaagtg gtagtacaga
     1741 ttcccgggac tgatgtcgac ttcgtcggcg atgtggttgg tcgtgacgtt gggcacgccc
     1801 agctcgttga acagccgcca gctcgcctcg aggatccgct cgcgcgtcgg attcggtcgg
     1861 gcgggccggc gggcgggccg ggcggccatc gcgtcgcgcc gggcctatcc ttggcctgcg
     1921 gcgcggcgcg ccgacacctt cttcgccggt tgcgtggcga cggcttggtc gggtgcgctc
     1981 gctgcgaccg acgccggcgc ggccgccgcc ggcgccttcc ttgcgccggt cttctttgcg
     2041 gcggtcttct ttgcgaccgg cttctccgca gccggcttcc ttgcagcggg cttctttgca
     2101 gccggcttcg ttgcagcggg cttcgttgca gcgggcttcg ttgcagcggg cttctttgcg
     2161 gccggcttcg ttgcagcggg cttctttgcg gcggtcttcc ttgctggggg cttctttgcc
     2221 gcgcgcttcg tcgccgcgcg cttcgtcgtc ttcccggcgg cctgcttcgc gccgacctgc
     2281 atcgcacgct cgagaaccgc cagctcctcg ccggcataga cggcgatctt ctgcgggccg
     2341 ggacagacct ccgggcaggc gatgatgccg acatcgaggc tgtcgtgata gctcgacgcc
     2401 gtgatgttga gcgcctgccc ggccagcagc agcgacgccg cgtacatgga ttcgagctcg
     2461 gcgccgttga cgtactggcg ctcgcgcggc ccgggcatgt tcgagatgat cagattcgcg
     2521 ttcagcacgc ggtgcccgaa gctcgtcttc gcggccagca tctgcgggat catcgacagc
     2581 tcggtgtaga tgccgtaggc ctcggtgccc agctccttca tctgcccctt gttcacccgg
     2641 ctcgattcga ccaccgccag caggcgcttc ttcgggttgg cgatgttcgt tcccagcgac
     2701 acgaggatct gcgccacggc gttccctgcc gtcgtgtcgt ccttgcgcgc caccgccacc
     2761 gggcaggccg cgatcagggg tttgtcgggc agcgccttga gctcggccag atagcgccgc
     2821 agcgcgccgc cgcagatggc gaggaagacc tcgttgatcg tcgcgccgcc ctcgatggcg
     2881 gcaccgagcg ccttgagccg atcgatcggc agcgagacgg tgccgagccg gcggcgttcg
     2941 gtcaggggct ggttcaggat cgattcgggc gcgctgtagg gcgcgatcac gccgtcgccc
     3001 gggacgttct ggcgcacgct cgacatcgcc cggagcgcct tgccccagtc gacgatcttg
     3061 cgcagggtcg actccgacgc cgccggaccg cccgcgcccg cctcgctcgc cggcggcttg
     3121 accgcccaca gcggcggcag gttgcgcgcc gacgccttgg acgagcggat gtagcggatc
     3181 aggtccatgc cgcgcttgcc gtcgaactgc gagtgatgca gcttcgtgta catcgcgtag
     3241 cgcccgcctt ccaggccttc gatcaggtgg acttcccaca gcgggcgcga tcgatcgagc
     3301 cgggcgacat gcagccgcga caccagttcg cccagttcgc gctggccgcc cggcctgggc
     3361 agcgcgtgca gaaagacgtg gtcggcggtg tccacgtcgg tcaacgtctc ccagccgcgc
     3421 tggggcttgg gcttgcggcg ccgctgcgtc ggcagcttca gccagtcggt ctggctctcc
     3481 ttgggcggct cggccgtgac gag
//
LOCUS       DAOQJH010000253         3490 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_17184_length_3490_cov_5.137409, whole genome shotgun sequence.
ACCESSION   DAOQJH010000253 DAOQJH010000000
VERSION     DAOQJH010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3490)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3490)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3490
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17184_length_3490_cov_5.137409"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..340)
                     /locus_tag="PLQ18_06820"
     CDS             complement(<1..340)
                     /locus_tag="PLQ18_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012432425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNE01404.1"
                     /translation="MADDTRDTPTPPPATGPAGSALHAAIAEYGVIALVLQGGGALGS
                     YQGGVAQGLLEAGVEPNWVAGISIGALNAAIIAGNAPQHRVARLREFWDTICRSAHFQ
                     PTAEFLQGWVE"
     gene            complement(451..1233)
                     /locus_tag="PLQ18_06825"
     CDS             complement(451..1233)
                     /locus_tag="PLQ18_06825"
                     /EC_number="1.1.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006162745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HNE01405.1"
                     /translation="MSLHGKVALITGAASGIGRNTAETLAAAGAAVAIADIRIEAAEQ
                     TAAEIRAATGSPAFGLAMDVTDEQAVGHGTQAVIERLGGLDILVSNAGIQIVSPIEDF
                     AFSDWKRMLAIHLDGAFLTTRAALRHMYAHGGGTVIYMGSVHSHEASPLKSAYVAAKH
                     GLLGLARVLAKEGAAHNVRAHVVCPGFVRTPLVDKQIPEQAKELGISEEDVVKHVMLG
                     ETVDAQFTAVEDIGTMIRFLCEFPSTALTGQSFIVSHGWHMQ"
     gene            1455..>3490
                     /locus_tag="PLQ18_06830"
     CDS             1455..>3490
                     /locus_tag="PLQ18_06830"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNE01406.1"
                     /translation="MTPAHRALRRLILAFAVLTLIGVGAYWFVLLRDLDTQVETEAAH
                     LGEHAEYRALAAAESLDATLRQLDAVLTGARQDWIDEAGDFDALAQAALRALPPGLAI
                     QLFVIGTDGLLKWSSLGPAPANRLGDRDYFRRLAAAVDDPLEISEPVIGRLTGRWSIQ
                     FARRIERHGRFAGVVALSVSPERWARDIEKYAPSDGDSLALFRPDGAYLLRMPDPERH
                     YGQRVPADRPFLGGDPNQHVYTTAAVVDGLVRTYAWRRLDAGPIVVAGIGYEAALAPA
                     RAFAQRQILQAVLGTGLLLACAIVLRVLMRRADQATRALAESAERQRAVLSSMAEGVM
                     ILAPGGRIEYANAAACRELGLPSERELYHPADTVELDMLRPDGSPMPVEDYPSRRSAL
                     SGADFDNELLGRRAADGSVRWFTITTRALHRERSRAPYMVLMTFRDITALRSAEAEAR
                     LSQTVFEAAAEGILVTDGEHRIIAANPAFERLSGHRLDELCGKTPQLLTADLPQPVTW
                     AEVLDALATHDGWEGEGLIRRADARALAVLIHVTVLRDGAGRPWRHVALFSDITQRKR
                     REDEAWQRAHYDVLTGLPNRRLLDERLQRALALAEPGRHELAVLFVDLDRFKPVNDVH
                     GHALGDALLERVARRLEAAVREEDTVARYGGDEFVIAMLMPPGAPAAETVAVKLV"
BASE COUNT          479 a         1257 c         1251 g          503 t
ORIGIN      
        1 gctcgaccca gccttgcaaa aattcggccg tcggctggaa gtgcgccgag cggcagatgg
       61 tgtcccagaa ctcgcgcagc cgggccacgc gatgctgggg ggcgttgccc gcgatgatgg
      121 cggcgttcag cgcgccgatc gagatgccgg ccacccagtt cggctcgacg ccggcctcca
      181 gcaggccctg cgcgacgccg ccctgatacg aaccaagcgc gccgccgccc tgcagcacca
      241 gggcgatgac gccgtactcg gcaatggcgg cgtgcagcgc actgccggcc gggccggtcg
      301 ccggcggcgg ggtgggggtg tcgcgggtat cgtcggccat gcggggcagt ctcgtgcgaa
      361 caggggatcc agcggcccgg gggtctcgtg cccaccgtag cgcaggccgg cgccggacgc
      421 gccggcgggt ctgcgtgccg gctgcggggt ctactgcatg tgccagccgt ggctgacgat
      481 gaagctctgg ccggtcagcg ccgtgctcgg gaactcgcac aggaagcgga tcatcgtgcc
      541 gatgtcctcg accgcggtga actgcgcatc gacggtctcg ccgagcatca cgtgcttcac
      601 gacgtcctcc tcgctgatgc cgagctcctt cgcctgctcc ggaatctgct tgtcgaccag
      661 cggcgtgcgc acgaagcccg ggcagacgac gtgggcgcgc acgttgtggg ctgcgccttc
      721 cttcgccagc acgcgcgcca gcccgagcag gccgtgcttg gcggcgacgt aggccgattt
      781 gagcggcgag gcctcgtgcg aatgcaccga gcccatgtag atcaccgtgc cgccgccgtg
      841 cgcgtacatg tgccgcaggg cggcgcgcgt ggtcaggaag gccccgtcga gatggatcgc
      901 cagcatcctc ttccagtcgg agaacgcgaa gtcctcgatc gggctgacga tctggatgcc
      961 ggcgttcgag accaggatgt cgaggccgcc gaggcgctcg atgacggcct gcgtgccgtg
     1021 cccgaccgcc tgctcgtcgg tcacgtccat cgccagaccg aaggcggggc tgccggtggc
     1081 ggcccggatt tcggcggcgg tctgttcggc ggcttcgatg cggatgtcgg cgatcgcgac
     1141 cgcggcgccg gcagcggcca gggtttcggc ggtgttgcgg ccgatgccgc tcgcagcacc
     1201 ggtgatcagg gcgactttac cgtggaggct catgggggat tcctcttgcg ggtggacgaa
     1261 agcgcgaccg gccgggtgcc cggattcaat tgcaagaccc ggaccggcaa gacccggacc
     1321 gcaaaccgcg gcgatcttcg tgatgcggcc atgccgcccg gaccccggat cgaggctgca
     1381 gccgcggccg tgcctgcagg caccgaccgc cggataccga gaccgttgtc ttaagcctgg
     1441 cttacgctag gcacatgact cctgcgcacc gcgccctgcg ccgcctgatc cttgcctttg
     1501 ccgtgctgac cctgatcggt gtcggtgcct actggtttgt gctgctgcgc gatctcgata
     1561 cccaggtcga gaccgaagcg gcgcacctcg gcgagcacgc cgaataccgg gcgctggccg
     1621 cggccgagag cctcgacgcg acgctgcgcc agctcgatgc cgtgctcacc ggtgcacggc
     1681 aggactggat cgacgaggcc ggcgacttcg atgcgctcgc acaggcggcg ctgcgcgcgc
     1741 tgccgccggg gcttgcgatc cagctgttcg tgatcggcac cgacggcctg ctcaagtggt
     1801 cgagcctggg tccggcgccg gcgaaccggc tcggggatcg tgactacttc cgccgcctcg
     1861 ccgcagccgt ggatgacccg ctggagatca gcgaaccggt gatcggccgg ctgaccgggc
     1921 gctggagcat ccagttcgcg cggcgcatcg agcgccacgg ccgcttcgcc ggcgtcgtgg
     1981 cgctgtcggt gtcgcccgag cgctgggcac gcgacatcga gaagtacgcc ccctcggacg
     2041 gcgattcact ggcgctgttc cggcccgacg gggcctatct gctgcgcatg cccgaccccg
     2101 agcgccacta cggccagcgc gtgccggccg accggccctt tctcggcggc gacccgaacc
     2161 agcacgtcta cacgaccgct gccgtcgtcg acggtctggt gcgcacctat gcgtggcggc
     2221 gcctcgatgc cgggccgatc gtggtcgccg gcatcggcta cgaggccgcg ctcgcgccgg
     2281 cgcgcgcctt cgcgcagcgc cagatcctgc aggccgtgct cggcaccggc ctgctgctcg
     2341 cctgcgcgat cgtgctgcgc gtgctgatgc gccgcgccga ccaagccacg cgcgcgctcg
     2401 ccgaaagcgc cgagcgccag cgcgccgtgc tgtcgtcgat ggccgagggc gtcatgatcc
     2461 tcgcgccggg cgggcgcatc gagtacgcca acgccgctgc ctgccgcgaa ctcggcctgc
     2521 cgagcgaacg cgagctgtac cacccggccg ataccgtcga gctcgacatg ctgcgcccgg
     2581 acggcagccc gatgccggtc gaggactatc cgagccgccg cagtgcgctc agcggcgccg
     2641 acttcgacaa cgaactgctc ggccggcgcg ccgccgacgg cagcgtgcgc tggttcacga
     2701 tcacgacgcg cgcgctgcac cgcgagcgca gccgcgcgcc gtacatggtg ctgatgacct
     2761 tccgcgacat cacggcgctg cgcagcgccg aggccgaggc gcggctgagc cagaccgtgt
     2821 tcgaggccgc ggccgaaggc atcctcgtca ccgacggcga gcaccgcatc atcgccgcca
     2881 acccggcctt cgagcgcctg agcggccacc ggctcgacga gctgtgcgga aagacgccgc
     2941 agctgctcac cgctgatctg ccgcagccgg tgacctgggc cgaggtcctc gatgcgctgg
     3001 ccacgcatga cggctgggaa ggcgagggcc tgatccggcg tgcggacgcc cgggcgctgg
     3061 cggtgctgat ccacgtcacc gtgctgcgcg acggcgccgg ccggccgtgg cggcatgtgg
     3121 cgctgttcag cgacatcacg cagcgcaagc gccgcgagga cgaggcctgg cagcgcgcgc
     3181 actacgacgt gctgaccggc ctgccgaacc gccgcctgct cgacgagcgc ctgcagcgcg
     3241 cgctggcgct ggccgaaccc ggccggcacg agctcgccgt gctgttcgtc gatctcgacc
     3301 gcttcaagcc ggtcaacgat gtccacggcc acgcgctcgg cgacgcgctg ctcgaacgcg
     3361 tcgcgcgccg gctcgaagcc gcggtgcgcg aggaggacac ggtcgcgcgc tacggcggcg
     3421 acgagttcgt gatcgcgatg ctgatgccgc ccggtgcgcc cgccgccgaa accgtcgccg
     3481 tcaagctggt
//
LOCUS       DAOQJH010000254         3476 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_17293_length_3476_cov_3.738965, whole genome shotgun sequence.
ACCESSION   DAOQJH010000254 DAOQJH010000000
VERSION     DAOQJH010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3476)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3476)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3476
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17293_length_3476_cov_3.738965"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3298)
                     /locus_tag="PLQ18_06835"
     CDS             complement(<1..3298)
                     /locus_tag="PLQ18_06835"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HNE01407.1"
                     /translation="MSLGTFLNEGIPAIFERLRTVYDIDFNYYKPGTITRRIERRVAL
                     HPEHIEIDEYVRHVEQDDAELDLLYKDLLIGVTRFFRDPEAFEILRTRVVPGIVERAP
                     EDEDVRVWVCGCSTGEEAYSLAILFLEAFAAAGRAPRIKILATDIHRKSLLTAGEGVY
                     PESSLTEMPEALRQRYFIEQPDRTWRVTANLRKALIFSEHNLLKDPPFTRIDLVSCRN
                     LLIYLEPPAQLRAIAAFHFALRVEGCLYLGASEGLGELAIEFGIVDRHWKIFTKLRDN
                     RLLHDLRLPLGYSTKSSLRALPGIGETRLGRIYDVLLTQFIPTGILVNDRYEAVHVFG
                     DASRYLLPPSGKVTSDLFSMVGGHLRIALMTALRNAQQQNSAVHYRGIVHESSGGERR
                     FLNVGVQPLHDTTLNTRYYMVRIDEAASLPVSSSGTAADVQVAGRPLDVDAQTYAQIR
                     VLELELQQARESLQSTIEELETSNEELQATNEELLASNEELQSTNEELHSVNEELYSV
                     NAEHELKIQELNAVTSDLNNLISSTELATLFIGADFRLRLFTPQATTIFPLLPQDIGR
                     DLRHFKPVETDEHLLDDVRQVLKTHAAVERTLAWSRERTFLRRITTYQDTNKAIAGVV
                     LTYVDISETARLGRELDRQRLRLEAVVDTSPNGLLIVDAEGRITMANPALARIFGYSH
                     GALDGHRVEDLLPPEQREAHIALRASYLRAPMTRAMGQGRELLGLRRDGSRFPVEVSL
                     AAFRTDDGDFVQATVIDISERRRAEETVRVAQLENARLAALIEATGDAIIGTDELGAI
                     TSWNPGARALLGHAPATVLGQPLASLFPDGDTGGSAMIAEIHGSQSLRRDCVLRHADG
                     HDVDVAVTISPVRSAAGRLLGLAVVAHEAGERIRRAAERERQREQLEREVEARTQELR
                     TLNVQLGEALASAEAATRAKSAFLANMSHEIRTPLNAITGMAYLLKESITDAGQRSQI
                     NKIERAGHHLVEIINSILDLSKIETGKVALEAEELSLEAIGNDVATMCGPSARARGLE
                     LRSDYAPQTKALRGDATRLRQAWLNYAANAVKFTEKGSVTLRTRLLDDESERACVRFE
                     VEDTGIGIDPA"
BASE COUNT          471 a         1141 c         1275 g          589 t
ORIGIN      
        1 cggccggatc gatgccgatg ccggtgtcct cgacctcgaa gcgcacgcag gcgcgttcgg
       61 actcgtcatc gagcaggcgg gtgcgcagcg tcaccgagcc tttttcggtg aacttcacgg
      121 cgttggccgc gtagttcagc caggcctgac gcaggcgcgt ggcgtcgccg cgcagcgcct
      181 tcgtctgcgg cgcgtaatcg ctgcgcagct ccagcccgcg cgcgcgcgcc gacggcccgc
      241 acatcgtcgc cacgtcgttg ccgatcgcct ccagcgacag ttcctcggct tcgagcgcga
      301 ccttgccggt ctcgatcttc gagagatcga ggatgctgtt gatgatctcg accagatggt
      361 ggccagcgcg ctcgatcttg ttgatctgcg accgctggcc ggcgtcggtg atgctctcct
      421 tgagcaggta ggccatgccg gtgatcgcgt tcagcggcgt gcggatctcg tggctcatgt
      481 tggcgaggaa ggcgctcttg gcccgggtcg cggcctcggc actggcgagc gcctcgccga
      541 gctggacgtt gagggtgcgc agctcctgcg tgcgcgcctc gacctcgcgt tcgagctgct
      601 cgcgctggcg ctcgcgctcg gcggcgcggc ggatgcgctc gccggcctcg tgcgcgacca
      661 cggccaggcc gagcagccgg cctgcggcac tgcgcaccgg cgagatcgtc accgccacat
      721 cgacatcgtg gccgtcggcg tggcgcagca cgcagtcgcg gcgcagcgac tggctgccgt
      781 gaatctcggc gatcatggct gagcccccgg tgtcgccatc cgggaacagc gaggccagcg
      841 gctggccgag caccgtcgcc ggtgcatggc cgagcagcgc gcgggcgccg ggattccagc
      901 tcgtgatcgc gccgagttcg tcggtgccga tgatcgcgtc gccggtggcc tcgatcagcg
      961 ccgcgagccg ggcgttctcg agctgcgcga cgcggacggt ttcctcggcg cggcgacgct
     1021 cgctgatgtc gatgaccgtg gcctgcacga agtcgccgtc gtcggtgcgg aaggccgcga
     1081 ggctgacctc gaccgggaag cggctgccgt cgcggcgcag gccgagcaac tcgcgcccct
     1141 gccccatcgc ccgcgtcatc ggggcgcgca ggtagctcgc gcgcagggca atgtgggcct
     1201 cgcgctgctc gggcggcagc aggtcctcga cccgatggcc gtcgagcgca ccgtgcgagt
     1261 agccgaagat gcgcgccagc gccgggttgg ccatcgtgat gcggccctcg gcatcgacga
     1321 tcagcagacc gttcgggctg gtgtcgacga cggcttcgag ccgcaggcgc tggcggtcga
     1381 gttcgcgccc gaggcgggct gtctcgctga tgtcgacgta ggtcagcacc acgccggcga
     1441 tggccttgtt ggtgtcctgg taggtcgtga tgcggcgcag gaaggtgcgc tcgcggctcc
     1501 aggccagcgt gcgctcgacg gcggcgtggg tcttgagcac ctggcgcacg tcatcgagca
     1561 ggtgctcgtc ggtctccacc ggcttgaaat ggcgcaggtc gcggccgatg tcctgcggca
     1621 gcagcgggaa gatcgtggtc gcctgcggcg tgaacagccg cagccggaag tcggcaccga
     1681 tgaacagcgt cgccagctcg gtcgagctga tcaggttgtt caggtcgctg gtgacggcgt
     1741 tgagctcctg gatcttcagc tcgtgctcgg cgttgacgct gtagagctcc tcgttgaccg
     1801 agtgcagctc ctcgttggtg ctctgcagct cctcgttgga ggccagcagt tcctcgttgg
     1861 tggcctgcag ttcctcgttg ctggtctcga gttcctcgat cgtgctctgc agcgactcgc
     1921 gcgcctgctg cagttcgagt tcgagcacgc ggatctgcgc gtaggtctgc gcatcgacat
     1981 cgagcggacg gccggcgacc tgcacatcgg cggccgttcc cgagctgctg accggcaggc
     2041 tggcggcctc gtcgatgcgc accatgtagt agcgcgtgtt cagcgtcgtg tcgtgcagcg
     2101 gctgcacgcc gacgttcagg aagcgccgct cgccgccgct gctctcgtgg acgatgccgc
     2161 ggtagtgcac ggccgagttc tgctgctggg cgttgcgcag cgccgtcatc agcgcgatgc
     2221 gcaggtgccc gccgaccatc gagaacaggt cgctggtgac cttgcccgag ggcggcagca
     2281 ggtagcgcga ggcatcgccg aacacgtgca cggcctcgta gcggtcattg acgaggatgc
     2341 cggtcgggat gaactgcgtc agcagcacgt cgtagatgcg tccgagccgg gtctcgccga
     2401 tgccgggcag ggccctcagc gaactcttgg tgctgtagcc gagcggcagg cgcaggtcgt
     2461 gcagcaggcg gttgtcgcgc agcttcgtga agatcttcca gtgccggtcg acgatgccga
     2521 actcgatggc cagctcgccg agcccctcgc tcgcgccgag gtacaggcag ccctcgacgc
     2581 gcagcgcgaa gtggaaggcc gcgatcgcgc gcagctgcgc cggcggttcg agatagatca
     2641 gcaggttgcg gcagctgacc agatcgatgc gcgtgaacgg cgggtccttc agcaggttgt
     2701 gctcggagaa gatcagcgcc ttgcgcaggt tcgcggtcac gcgccaggtg cggtcgggct
     2761 gctcgatgaa gtacctctgg cgcagcgcct ccggcatctc ggtcaggctg ctctccgggt
     2821 agacgccctc gccggcggtc agcagcgact tgcggtggat gtcggtggcg aggatcttga
     2881 tgcgcggcgc gcgcccggcc gccgcgaagg cttcgaggaa caggatcgcc agcgagtagg
     2941 cttcctcgcc ggtcgagcag ccgcagaccc agacgcgcac gtcctcgtcc tcgggagcgc
     3001 gctcgacgat gccgggcacg acgcgcgtgc gcaggatctc gaaggcctcg gggtcgcgga
     3061 agaagcgcgt gacgccgatc agcaggtcct tgtacagcag gtcgagctcg gcatcgtcct
     3121 gctcgacgtg gcgcacgtac tcgtcgatct cgatgtgctc ggggtgcagc gcgacgcgcc
     3181 gctcgatgcg ccgggtgatc gtgccgggct tgtagtagtt gaagtcgatg tcgtagacgg
     3241 tgcgcaggcg ttcgaagatc gccgggatgc cctcgttcag gaacgtcccg agggacaacg
     3301 cgtacccctg gagctgggtg aaggcgctcg ggccaggcgg gtcggcccag tccaggccgg
     3361 acccgcccgg ccggggggtg atgacccggt cggtacgggc cgcccggttg aactctcccg
     3421 ggaacggggc cccgacctcc tggctggccg tcagccggga ccagaactcc ggcggg
//
LOCUS       DAOQJH010000255         3475 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_17304_length_3475_cov_3.397368, whole genome shotgun sequence.
ACCESSION   DAOQJH010000255 DAOQJH010000000
VERSION     DAOQJH010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3475)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3475)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3475
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17304_length_3475_cov_3.397368"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2107)
                     /locus_tag="PLQ18_06840"
     CDS             complement(<1..2107)
                     /locus_tag="PLQ18_06840"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HNE01408.1"
                     /translation="MPTATAQALVTASADGLRARVEGAAGAGADWTASREEAARRLRD
                     WAERHDRLAGLDDADSELLEIGREMARWLDDSGWLSGWLRRPGARRLILQSPDVEHDL
                     GRLLHDAPWELLTAPGAESGHLAGDPLRPLELARRLGAPEDPQQPVHGDLCLLFMAAA
                     PQGETVLDFEAEESSILEATRSLHQLLLTVEESGSLAGLQQRLCASEGVAPDIVHLSC
                     HGGVDDGLPVLYLEDERGQRQAVPAARLAEVLSASPELRLVVLSACGTAAQTSDGEGR
                     RSTLVTDLICSGVDQVLGWDGSVADRDATQFAAEFYKALTARQDVVAAAARARLRLYT
                     EHAQEARCGRHWHMARVYLGPRGGQTLLAQGGQRRALRSAVGEEHFLDKARAAVPVAT
                     RSHFVGRRRAIQQVLGAFEDGRAALVHGMGNLGKSSLAARIAHDRLPQLACVVVFGRY
                     DALEVWDRLLDACTPAERPPFRDRWRSRIAADERQLADALDDLLAGPLAARPVLLIID
                     DLERVLEEPRPDQPSVRVQPERRPMLAAVLRAFGRQQRSKLLLTSRYTFSLPGSGGRD
                     LAADLVLVPLRAMDESDRRKQQRALLRDLPERQVARLDLALLQRAGELADGNPGLQAL
                     LSQPVLAGEAQAAGQAFAVIEHYLQHGVPPAAIQHELASGRAADERNAVLAFFRRMAF
                     DTYRNALRSEQLRALQAGCL"
     gene            complement(2201..3337)
                     /locus_tag="PLQ18_06845"
     CDS             complement(2201..3337)
                     /locus_tag="PLQ18_06845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE01409.1"
                     /translation="MSNLHKDTLRPMRRRGLDSHARNGPFSDSTASGHEQAAHAYVRT
                     GGGSDRAKRVENHEYAPDVVCHQLEWTASDSQQGGVPILRGTADSGRLPAISRRLAGM
                     ARDHVQEASVMSAHAYLSQLQLAPVAVRATISDSTTHALRSGAGSAEAAADTAGIPLT
                     DVRAAVDVGSLVSFVDGLDAQSKDDVLDSLQLAQRAANAAADRFADVRNWYHQYLDVL
                     GQIGWVTGQFAFLEREQSEGDLRMDQAALSVLMAIATQNQLAILKEAVSALGSLASSD
                     GTISLFAQMSAHEGLGNFQMGAAQRAGNGVVELAMGAYHFRAVDSTDRIVFFKRSTRA
                     LKLWTAAQRMTLNTASYAENRAQIRQMLGEERQRAIAAIRLKPL"
BASE COUNT          480 a         1191 c         1250 g          554 t
ORIGIN      
        1 acaggcagcc ggcctgcagc gcgcgcagct gctcgctgcg cagcgcattt cggtaggtgt
       61 cgaaggccat gcgccggaag aaggcgagca cggcgtttcg ctcgtcggcc gcgcggccgg
      121 atgccagttc gtgctggatc gccgccggcg gcacgccgtg ctgcagatag tgctcgatga
      181 cggcaaacgc ctgcccggcg gcctgcgcct cgccggcgag caccggctgg ctgagcagcg
      241 cctgcagtcc ggggttgccg tcggccagct cgccggcgcg ctgcagcagg gcgaggtcga
      301 gacgagcgac ctgccgctcc ggcaggtcgc gcagcaacgc acgctgctgc ttgcgccggt
      361 cgctctcgtc catggcgcga agcggcacga gcaccaggtc ggcggcgagg tcgcgcccac
      421 ccgagccggg caggctgaag gtgtagcggc tggtcagcag cagcttgctg cgttgctgcc
      481 ggccgaaggc tctgagcacc gcagcgagca tcggccgccg ttcgggctgg acccggacgc
      541 tcggctggtc gggccgtggt tcttcgagca cgcgctcgag atcgtcgatg atcagcagca
      601 ccggccgcgc ggcgagcggg ccggcgagga ggtcgtcgag cgcgtcggcc agttgtcgct
      661 cgtcggcggc gatccggctg cgccagcgat ccctgaatgg cggccgctcg gcgggcgtgc
      721 aggcgtccag caggcggtcc cagacttcca gcgcgtcgta gcggccgaac accaccacac
      781 aggcgagctg cggcaggcgg tcgtgtgcga tgcgcgcggc caggctcgac ttgccgaggt
      841 tgcccatgcc gtgcaccagc gccgcccggc cgtcctcgaa ggcgccgagc acctgctgga
      901 tcgcgcgccg ccggccgacg aagtggctgc gcgtcgccac cggcaccgcg gcgcgtgcct
      961 tgtcgagaaa atgttcttcg ccaacggcgc tgcgcagcgc ccgccgctgc ccgccctggg
     1021 cgagcagcgt ttgcccgcca cgcggcccga ggtagaccct tgccatgtgc cagtgccggc
     1081 cgcagcgtgc ttcctgtgca tgctcggtgt acagccgcag ccttgccctg gccgcggcag
     1141 cgacgacatc ctgccgcgcg gtcagcgcct tgtagaactc ggcggcgaac tgcgtcgcgt
     1201 cgcggtcggc caccgacccg tcccagccga gcacctgatc gacgccgctg cagatcaggt
     1261 cggtcaccag cgtgctgcgt cgcccttcgc cgtcgctggt ctgcgccgcc gtgccacagg
     1321 cggagagcac gaccagccgc agttccgggc tggcggacaa gacctcggcg agtcttgccg
     1381 cgggcaccgc ttgccgctgg ccgcgttcgt cttcgaggta cagcacgggc aagccgtcgt
     1441 ccacgccgcc gtggcagctg aggtggacga tgtccggcgc gacgccttcg ctggcgcaca
     1501 ggcgctgctg cagaccggcg agcgagccgc tttcctcgac ggtcagcagc agttgatgca
     1561 ggctcctggt agcctcgagg atgctggatt cctcggcttc gaagtcgagc accgtttcac
     1621 cctgcggcgc ggccgccatg aacagcaggc agagatcgcc gtgcacgggc tgctgcgggt
     1681 cttccggggc gccgaggcgg cgtgccagct cgagcggccg cagcgggtcg cccgccaggt
     1741 ggccggactc ggcacccggc gcggtcagca gctcccacgg ggcgtcgtgc agcaggcgtc
     1801 ccaggtcgtg ctcgacatcg ggcgattgca ggatcagccg gcgcgcgccg ggacggcgca
     1861 gccagccgct cagccagccg gaatcgtcga gccagcgcgc catttcgcgg ccgatctcga
     1921 gcagctcgct atccgcgtcg tccaggccgg ccagccgatc atggcgttcg gcccagtcgc
     1981 gcagccgtcg cgcagcctcc tcgcggctgg cggtccagtc ggcgcccgcg ccggccgcgc
     2041 cttccacgcg ggcacgcaag ccatcagcgc tggcggtgac gagggcttgc gcggttgcag
     2101 tcggcatggt ggtgggttcc tttcggtttg atcgatgtct gttgctgctc tctgtcggaa
     2161 acacgcggcg cggcggcaac cgatgcccgt gccgggccga tcagagcggc ttgagccgga
     2221 tcgccgcgat cgcccgctgg cgttcctcgc cgagcatctg ccggatttgc gcccggtttt
     2281 ccgcatagct ggcggtgttc agcgtcatcc gctgggcagc cgtccacagc ttcagcgccc
     2341 gtgtgctgcg cttgaagaag acgatccggt cggtgctgtc cacggcccgg aagtggtagg
     2401 cgcccatcgc cagctcgacc acgccgttgc cggcacgctg cgccgcgccc atctggaagt
     2461 tgccgagccc ctcgtgggcg ctcatctgcg caaacaggct gatggtgccg tcgctgctgg
     2521 ccagcgagcc gagcgcggat accgcctcct tcaggatggc cagctggttc tgcgtcgcga
     2581 tcgccatcag gacggacagc gcggcctggt ccatgcgcag gtcgccttcg ctctgctcac
     2641 gttccaggaa ggcgaactgc ccggtcaccc agccgatctg gccgagcacg tcgaggtact
     2701 ggtggtacca gttgcgcaca tcggcaaagc ggtcggcggc agcgtttgcc gcacgctgcg
     2761 caagttgcag ggaatcgagc acgtcgtcct tgctctgcgc gtcgagtccg tcgacgaagc
     2821 tcaccaggct gccgacatcg acggcggcgc gcacatcggt gagcggtatc ccggccgtgt
     2881 ctgctgcggc ttcggcactg cccgcaccgg aacgcagcgc atgcgtcgtc gaatcggaga
     2941 tcgtggcgcg cacggcgacg ggcgcgagtt gcaactgtga gagataggca tgtgccgaca
     3001 tgaccgaggc ctcctgaacg tgatcgcggg ccatgccggc gaggcgacgg ctgatggccg
     3061 gcaagcggcc agagtccgcc gtcccgcgca ggattggcac gcctccctgc tgcgaatccg
     3121 atgccgtcca ttctagctga tggcacacga catctggcgc gtattcgtgg ttttcgacgc
     3181 gtttggctcg atccgatccg ccgccggttc gaacgtacgc gtgggcagcc tgttcatggc
     3241 cggaggcggt ggagtcggaa aacgggccat ttctggcatg gctgtccagg cctcgtctgc
     3301 gcatcgggcg cagcgtatcc ttgtggaggt tggacatgaa gagagtgaat cggcattacc
     3361 cgccggagtt tcggaaggcg tcggtgcaac aggtggttga tggcgggcgc agcgatccgg
     3421 cggtggcgcg gtcgttggag atgtcggcga agacgttggc gaactgggtc gcgcg
//
LOCUS       DAOQJH010000256         3463 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_17405_length_3463_cov_4.286092, whole genome shotgun sequence.
ACCESSION   DAOQJH010000256 DAOQJH010000000
VERSION     DAOQJH010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3463)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3463)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3463
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17405_length_3463_cov_4.286092"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            488..892
                     /locus_tag="PLQ18_06850"
     CDS             488..892
                     /locus_tag="PLQ18_06850"
                     /inference="COORDINATES: protein motif:HMM:NF024600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-hand domain-containing protein"
                     /protein_id="HNE01410.1"
                     /translation="MRNNKNSSLIAATLTLALGAGAAWAANPVNPGDAPTTTPSGRTP
                     SGQVMPGGSEGAKTDATVVPQPVDKTMFPTFAQADLNKSGTIEPTEIGMVKGLDFQKA
                     DVDHNGTLSKTEYEQAVQSATAPAGKLKDSRG"
     gene            1061..1969
                     /locus_tag="PLQ18_06855"
     CDS             1061..1969
                     /locus_tag="PLQ18_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003611522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNE01411.1"
                     /translation="MDSLTGLRVFVKVVETGSFTAAAEALELSRAMVSKHVQTLEARL
                     GVRLLQRSTRRVALTEAGSRYHARASAWLEDMDTTEAELGQLITEPRGTLRISAPMSF
                     GRVYVAPLLGEFLNRHPGLRIDLALADRAVDLIEDGYDLAVRIGELADSSLVCRRLAA
                     TRLLLCASPGYLARYGTPDHPAELSGHECLSYRLSDTPSDWHFSDADGHTATVRIDGR
                     VRADNGEALCEAAANGLGIVLQPDFIAGPFLQAGRLLALLPDWNTREIGIHAVYPERR
                     YLPARARRFIDFLAERYALAPWRMES"
     gene            1966..2568
                     /locus_tag="PLQ18_06860"
     CDS             1966..2568
                     /locus_tag="PLQ18_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003265247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HNE01412.1"
                     /translation="MSTKPVIGLLLAAGLSSRYRAVSGRHKLLEAVPDGLPGAGTPLA
                     LCAARSLRAGTDAVLAIVRPEDEPLAGLLAASGCEVLGFASPGLGASLAFGVRARPEA
                     GGWLVLPADLPFVRPSTVQAVRAALAVSPCAAPAYRNQRGHPVGFGARHYAALAALDG
                     DRGARDLLETQGWQRIGVDDPGCVADIDVPADLEACLRPR"
     gene            complement(2765..2908)
                     /locus_tag="PLQ18_06865"
     CDS             complement(2765..2908)
                     /locus_tag="PLQ18_06865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE01413.1"
                     /translation="MCPSPFVAVSHDSGLSAGHPMRRWVLRAWRFGLIGNRRAARMLS
                     RLA"
     gene            complement(3088..>3463)
                     /locus_tag="PLQ18_06870"
     CDS             complement(3088..>3463)
                     /locus_tag="PLQ18_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012639605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HNE01414.1"
                     /translation="DWSLPLLGRIAAGQPIEAVADEQTLDLRLLGGANRYALRVRGES
                     MIEAGILDGDTVIVEACEAARNGDIVVALIEGGEVTLKYYHPRPDGTVLLVPANARME
                     PMRFRADSVRVQGRVVGQLRLY"
BASE COUNT          518 a         1243 c         1161 g          541 t
ORIGIN      
        1 tcgatcgtcc tgctcgccac ccgtgccggt ccgctgccgg ccgcggtcgc caacttcgtc
       61 gagcaggcgg cgaccggtgc gcggctcggg gcaccaccaa ccaccggttg acgactggcg
      121 ggaatttcgt catcgctgcc gcaagcagta cctccgcggt ccgcttgccg tccgggcggc
      181 agttatccac agattgtctg gataattcct tcctctgagg cgcgctgcgc cgaaagcggc
      241 gtgtctgcac ggcgactttg caagtcattg atttttcgtg gagccatctc actccgccgc
      301 actgctgtcg ctgtttcccg acgggatgcc ggctgcccgc atgtggccgt ctcgcccgga
      361 acaaaacatc aagcaattgt attgaaacgg atttatctga catggcatgg atactggact
      421 actgccgctg ccaggccgga aaggccggcg gggaatgttc cccgatatcg ccacagaggt
      481 ttccgtcatg cgcaacaaca agaactcgtc cctgatcgct gcgaccctga ccctcgcgct
      541 cggcgccggt gccgcctggg cggccaatcc ggtcaacccc ggtgatgcgc cgaccacgac
      601 gccgtccggc cgtacgcctt ccggccaggt gatgccgggc ggttccgaag gtgcgaagac
      661 cgatgcgacc gtcgtgccgc agcccgtcga caagacgatg ttcccgacct ttgcccaggc
      721 cgacctgaac aagtccggca ccatcgagcc gaccgagatc ggcatggtca agggtctcga
      781 tttccagaag gccgacgtcg atcacaacgg cacgctgagc aagaccgagt acgaacaggc
      841 ggtccagtcg gccaccgcgc cggccggcaa gctcaaggat tcgcgcggct gacacggccc
      901 ccggtcgcgc gatacgccac tgccggggtt tcccggcagc ggcggcccct cgtcacggcc
      961 cgttcctcct gcgggggagc gggccgtaca cttgtcggtc cttcgcgcag caagacggct
     1021 ccggcggctt cggcccggac gccggggggc cttcccgatc atggactccc tcaccggcct
     1081 tcgggtcttc gtcaaggtcg tcgagaccgg cagcttcacg gccgcggccg aggcgctgga
     1141 gctgtcgcgg gcgatggtca gcaagcacgt gcagacgctg gaggcacgcc tcggcgtgcg
     1201 cctgctgcag cgctcgaccc ggcgcgtggc gctgaccgaa gccggcagcc gctaccacgc
     1261 gcgcgccagc gcctggctgg aggacatgga cacgaccgag gcggagcttg gacagctcat
     1321 caccgagccg cgcgggacac tgcgcatcag cgcaccgatg agcttcgggc gcgtctacgt
     1381 cgcgccgctg ctcggggagt tcctcaaccg tcacccgggc ctgcgcatcg acctggcgct
     1441 cgccgatcgc gccgtcgacc tgatcgagga cggctacgac ctcgccgtgc gcatcggcga
     1501 actcgccgac tccagcctcg tctgccggcg cctcgccgcc acccgcctgc tgctgtgcgc
     1561 ctcgcccggc tatctcgccc gctacggcac gccggaccat ccggccgagc tgtccggtca
     1621 cgaatgcctg agctaccggc tctccgacac gccttcggac tggcacttca gcgacgccga
     1681 cggccacacg gccaccgtgc gcatcgacgg ccgcgtgcgt gccgacaatg gcgaggcgct
     1741 gtgcgaggcg gccgcgaacg ggctcggcat cgtgctgcag ccggacttca tcgccggccc
     1801 tttcctgcag gccggccggc tgcttgcgct gctgccggac tggaacacgc gcgagatcgg
     1861 catccacgcc gtttacccgg aacgccgcta tctgccggcc cgggcccggc gcttcatcga
     1921 ttttctggcc gagcgctacg cgctggcgcc gtggcggatg gagtcatgag cacgaaaccg
     1981 gtcatcggcc tgctgctcgc cgccggcctc agcagccgtt accgcgccgt cagcggtcgg
     2041 cacaagctgc tcgaagcggt gccggatggc ctgccgggcg ccggcacgcc tctggcgctg
     2101 tgcgcggcgc gctcgctgcg ggccggcacg gacgcggtgc tggcgatcgt gcgaccggag
     2161 gatgaaccac tcgccggcct gctcgccgcg agcggctgcg aggttctggg cttcgcgagt
     2221 cccggcctcg gcgcgagtct cgcgttcgga gtgcgtgccc gcccggaggc cggcggctgg
     2281 ctggtgctgc cggccgacct gcccttcgtg cggccttcga ccgtgcaggc cgtgcgcgcc
     2341 gcactggcag tcagcccctg cgctgcccct gcataccgga accaacgtgg ccatccggtc
     2401 ggcttcggcg cacgacacta cgccgcactc gcggcgctcg atggcgatcg cggtgcgcgc
     2461 gacttgctgg aaacccaagg ctggcagcgg atcggggtcg atgatccggg ctgtgtggcc
     2521 gacatcgacg tgccggcgga tctggaagcc tgtctgcgcc cacgctgatg ccgcttcgcg
     2581 gggctttcag gcagcggacc gggcggtccg gaaaccggaa gggcgatgcc ctggaacaaa
     2641 cggtgcaaca agcggtgaac gcggtgcggc gtccgggagc gacaccgccc gggcctgcgt
     2701 cagccgctcg tggaaacccg gcgcccgcac gccggtgaat ccaccttcgg ctgcgcccgc
     2761 ggcctcacgc gaggcgcgac agcatgcgtg cggcgcggcg gttgccgatc aggccgaagc
     2821 gccaggcgcg caggacccag cgacgcatcg ggtgcccggc ggagagaccg gaatcatggg
     2881 atacggcgac gaacgggctc gggcacatgt ttgctgctcc tgtcttgatc ggaccggtga
     2941 cctcttgggc cccgtgaatg gatgccggtc gcgggggaac gcagccggca gacggacatt
     3001 tacgcatcgt tccccccatt ccatgcaagt catctgacaa cgatgcgacc ggaaatcacg
     3061 atccgggttg ccggtgtcag accggcgtta atacagcctt aactgaccca cgacgcgtcc
     3121 ctgcacccgt accgagtcag cccgaaagcg catcggctcc atgcgggcat tggccggcac
     3181 cagcagcacc gtgccgtccg ggcgcgggtg gtagtacttc agcgtcacct cgccgccttc
     3241 gatcagcgcg acgacgatgt cgccgttacg cgccgcttcg caggcttcga cgatcaccgt
     3301 gtcgccgtcg aggatgccgg cctcgatcat cgattcaccg cgcacgcgca gcgcgtagcg
     3361 gttggcaccg ccgagcaggc gcaggtccag cgtctgttcg tccgcgaccg cctcgatcgg
     3421 ctggccggcg gcgatgcgtc cgagcagcgg cagcgaccag tcc
//
LOCUS       DAOQJH010000257         3463 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_17407_length_3463_cov_3.918134, whole genome shotgun sequence.
ACCESSION   DAOQJH010000257 DAOQJH010000000
VERSION     DAOQJH010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3463)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3463)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3463
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17407_length_3463_cov_3.918134"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..999)
                     /locus_tag="PLQ18_06875"
     CDS             complement(<1..999)
                     /locus_tag="PLQ18_06875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE01415.1"
                     /translation="MAITYRLTTYGFQEGRPRGPARALMTGLLEGEPEDIERLADRVL
                     CGERLALAGGLDKADGERLRKALAGVGIEAKLYPESLSLLPVELEAPQAHTYRCPACH
                     HEQEVDPLAPDTCKSCGVIGSKFDEVREIKDVLDAERRKLEARISEAEAAEIEQQRQR
                     EHERLREIGRKRAEQQLGITRMTKLRALLRSPVFAPLAGGVAVAVLAGVGIWQYRVHQ
                     SPAPAAAQSSVAQIPGAVTPGPAPVQAVAADSSKRPQGAPPAEIEQAIAAAEAGTGGG
                     RPGLTPQQAVMAVNQLASKLPGGGEAGPAAAAGFTAVSVAGPEAVPPAAAAPAAALL"
     gene            1248..2186
                     /locus_tag="PLQ18_06880"
     CDS             1248..2186
                     /locus_tag="PLQ18_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014290865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HNE01416.1"
                     /translation="MPASAALLPVLAALGPVFGLIALGTVLRRCGFPGSGFWQPAERL
                     SYYLLLPALLLRELARADFGALAVGGVVVALLATIALMSVLALRLRRWLGNDGPAFGA
                     SFQGVIRLNTYVGLATALPLYGAAGGALAALALAVMVPVVNLLSVLVITHALGQARPA
                     HLLRQLAGNPLILACAAGIALNVSGLGLPPGSEGFTRILAQAALPLGLLAMGAALQLA
                     AVRGRAAAITVTVLLKLVLAPLLAAALARLCALPALESAVVVLFAALPSAPSAYILAR
                     QLGADAPLAAVVITVETLAAMLTLPLVVLLCAGPHS"
     gene            2183..>3463
                     /locus_tag="PLQ18_06885"
     CDS             2183..>3463
                     /locus_tag="PLQ18_06885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HNE01417.1"
                     /translation="MTAPQSQEPAAGVAGLPRGLRVTVIVGAVLVLAALVMLVAALLT
                     RQSALAELRAEAAPALQRSVSSLQGLLDTYQILPRVLAREPLILNLYAIPALSPATRE
                     TANEFLSAFNAAAGASETYVLDALGNTIAASNSESPVSFVGRNFAFRPYFHLAIAGWA
                     GLYFAHGTTSNRPGFYFSYPVKSSGGDAVGVAVVKIGQERLESAWSQSATPILVTDAA
                     GVVFITNREDFRYHALRPLPPVLAAELVGSRQYGDGPLAPLPVVGEREFDGVRLVSLH
                     EGRRSVEYLLAKAPVPNTDWEVSVLVPTATVEEQVGVAVLLAGSASAIATLFGLMLAQ
                     KRLRTHERLAYQARVAEALRESHDRLEAHVVERTRDLVEANQRLSREIQERERAEQIV
                     LKTQNELIQASKLAALGQLAAGIVHELNQPLAAIR"
BASE COUNT          390 a         1266 c         1274 g          533 t
ORIGIN      
        1 cagcagcgct gcggccgggg cagccgccgc aggcggtacg gcctccggcc cggccaccga
       61 caccgccgtg aagccggccg ccgccgcggg gccggcttca ccgccgcccg gcagcttcga
      121 tgccagctgg ttgaccgcca tcaccgcctg ctgcggtgtc aggcccggcc ggccaccgcc
      181 ggtgccggcc tcggccgcgg cgatcgcctg ctcgatctcg gccggcggtg cgccctgcgg
      241 acgcttgctg ctgtcggctg ccaccgcctg caccggggca ggacccggcg tcacggcgcc
      301 cggaatctgt gccacggacg actgcgcggc cgccggagcc ggcgactgat gcacgcggta
      361 ctgccagatg ccgacgccgg cgagcacggc gaccgccacg ccgccggcga gtggcgcaaa
      421 caccggactg cgcagcagcg cccgcagctt ggtcatgcgg gtgatgccga gctgctgctc
      481 ggcgcgcttg cggccgatct cgcgcaggcg ctcgtgctcg cgctggcgct gctgctcgat
      541 ttcggcggcc tcggcctcgc tgatgcgcgc ctccagcttg cgccgctcgg cgtcaagcac
      601 gtccttgatc tcgcgcacct cgtcgaactt gctgccgatc acgccgcagg acttgcaggt
      661 atcgggcgcg agcggatcga cttcctgctc gtggtggcag gcggggcagc ggtaggtgtg
      721 cgcctgcggt gcttcgagct cgaccggcag caggctcagg gattcgggat agagcttggc
      781 ctcgatgccg acgccggcga gcgccttgcg caggcgctct ccatccgcct tgtcgagacc
      841 gccggccagc gccagacgct cgccgcagag cacgcgatcg gcgagccgct cgatgtcttc
      901 gggttcgcct tccagcaggc cggtcatcag cgcgcgcgcc gggccgcgcg gccggccttc
      961 ctggaagccg taggtcgtga gtcggtaggt gatcgccatg actggcttgc tcctcgggct
     1021 gcgtcagtgc atccgccggg cggtgccgct gcgtgatgcc acggtccgtg tcgtttcctg
     1081 tgaggtttcg ccgatgcccg ggtctggccc cggccgggca cggcgaagtc ggggcaggct
     1141 tcaatatcac cggcctgtga cagggacaag cggcagccgg cgtagtgcag ccggccggag
     1201 cctcgcgggc atttcacatc gcgcaagcgc ttcgctacgc tgcatcgatg cccgcctccg
     1261 ccgccctgct ccccgtcctc gccgccctcg gcccggtgtt cgggctgatc gcgctcggca
     1321 ccgtgctgcg ccgctgcggc tttccgggca gcggcttctg gcagccggcc gagcggctga
     1381 gctactacct gctgctgccg gcgctgctgc tgcgcgagct ggcgcgcgcc gacttcggcg
     1441 cgctggccgt cggcggcgtg gtcgtcgcgc tgctcgcgac gatcgcgctc atgagcgtgc
     1501 tggcgctgcg gctgcgccgc tggctgggca acgacgggcc ggccttcggc gcgagcttcc
     1561 agggcgtgat ccggctcaac acctacgtcg ggctggcgac ggcgctgccg ctgtacgggg
     1621 ccgccggcgg cgcgctggcg gcgctcgccc tggcggtcat ggtgccggtc gtgaacctgc
     1681 tgagcgtcct cgtcatcacg cacgcgctcg ggcaggcccg gccggcccac ctgctgcgcc
     1741 agctcgccgg caacccgctg atcctcgcct gcgcggccgg catcgcgctg aacgtctcgg
     1801 gcctcggcct gccgccgggc agcgagggct tcacgcgcat cctcgcgcag gcagcactgc
     1861 cgctcgggct gctggcgatg ggtgcggcgc tgcagctcgc ggccgtgcgc gggcgggccg
     1921 cggcgatcac ggtgacggta ctgctgaagc tcgtgctggc gccgctgctc gccgccgcgc
     1981 tggcgaggct ctgcgcgctg ccggcgctgg agagcgccgt cgtcgtgctg ttcgcggcgc
     2041 tgccctcggc gccgtcggcc tacatcctcg cgcgtcagct cggggccgat gcgccgctcg
     2101 cggcggtggt gatcacggtc gagacgctgg cggcgatgct gacgctgccg ctggtcgtgc
     2161 tgctctgcgc agggccgcat tcatgacagc gccgcagtcg caggaaccgg cggccggcgt
     2221 cgctggcctg ccgcgcggtc tgcgcgtgac ggtgatcgtc ggtgccgtgc tggtgctcgc
     2281 ggcgctggtg atgctggtcg cggcgctgct gacgcgccag tccgccctcg ccgagctgcg
     2341 tgccgaggcg gcgccggcgc tgcagcgctc ggtcagcagc ctgcagggcc tgctcgacac
     2401 ctaccagatc ctgccgcgcg tgctggcgcg cgagccgctg atcctgaacc tgtacgcgat
     2461 cccggcgctg tcaccggcca cgcgcgaaac cgccaacgag ttcctgtcgg ccttcaacgc
     2521 cgcggccggg gcctccgaga cctacgtgct cgatgcgctc ggcaacacga tcgcggccag
     2581 caactccgaa tcgccggtgt ccttcgtcgg gcgcaacttc gcgttccggc cctactttca
     2641 tctcgcgatc gccggctggg ccgggctgta cttcgcgcac ggcacgacct cgaaccggcc
     2701 cgggttctat ttctcgtatc cggtcaagtc gagcggcggc gacgcggtcg gggtcgccgt
     2761 ggtcaagatc ggccaggagc gcctggaatc ggcctggtcg cagagcgcca cgccgatcct
     2821 ggtcaccgat gccgccggcg tggtgttcat caccaaccgc gaggacttcc gctatcacgc
     2881 gctgcggccg ctgccgccgg tgctggccgc cgagctggtc ggctcgcgcc agtacggcga
     2941 cgggccgctg gcgccgctgc cggtggtcgg cgagcgcgag ttcgacggcg tgcggctggt
     3001 cagcctgcat gagggccggc gcagcgtcga atacctgctg gcgaaggcgc cggtgccgaa
     3061 caccgactgg gaggtctcgg tgctggtgcc gacggcgacg gtcgaggagc aggtcggcgt
     3121 cgcggtgctg ctggccggct cggcgagcgc gatcgcgacg ctgttcgggc tgatgctggc
     3181 gcagaagcgc ctgcgcacgc acgagcggct cgcctatcag gcgcgcgtcg ccgaggcgct
     3241 gcgcgagtcg cacgaccggc tggaggcgca cgtcgtcgag cgcacgcgcg atctggtcga
     3301 ggccaaccag cggctgtcgc gcgagatcca ggaacgcgag cgcgccgagc agatcgtgct
     3361 gaagacgcag aacgagctga tccaggcgag caagctcgcc gcgctcggcc agctcgccgc
     3421 cggcatcgtc cacgagctga accagccgct ggcggcgatc cgc
//
LOCUS       DAOQJH010000258         3463 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_17410_length_3463_cov_3.572477, whole genome shotgun sequence.
ACCESSION   DAOQJH010000258 DAOQJH010000000
VERSION     DAOQJH010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3463)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3463)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3463
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17410_length_3463_cov_3.572477"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            257..1222
                     /gene="thiL"
                     /locus_tag="PLQ18_06890"
     CDS             257..1222
                     /gene="thiL"
                     /locus_tag="PLQ18_06890"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HNE01418.1"
                     /translation="MTAEFTLIDDLIARRTGAQRADVALGIGDDCALLVPPPGRQLAV
                     SLDLLVAGVHFLPDAEPVALGHKALAVNLSDLAASGAEPAWITLGLTLPGADAGWLAA
                     FMDGFAALAAEHGAQLVGGDTTRGPLCIAVQVHGFVPPGAALTRSGARAGDLVCITGT
                     PGEAAAGLALRFGRLELPEPFATHCLARLDRPTPRIRAGLALRGLASACIDVSDGLAQ
                     DLGHILSASGCGATLALGRLPLTAVSAALGAEAAWPYALAGGDDYELLFTLPAAQQAE
                     AEARLAAAGCPFSVIGRIDPEPGLRLLRPDGRPHALERGGYEHFA"
     gene            1206..1688
                     /locus_tag="PLQ18_06895"
     CDS             1206..1688
                     /locus_tag="PLQ18_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010323455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HNE01419.1"
                     /translation="MNTSPERAPRVFRDWRHFLAFGLGSGLVPVAPGTAGTLAAVPFA
                     WGLAALSPAAYLFIVLAAFALGVYVCGRTSRDLGVHDHGGIVWDEFVGYWITMYMAPP
                     GPGWLIAGFALFRLFDVWKPWPIRIFDVHVPGGLGIMLDDVLAGLYAWVLLQALTAVL
                     "
     gene            1817..2029
                     /locus_tag="PLQ18_06900"
     CDS             1817..2029
                     /locus_tag="PLQ18_06900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE01420.1"
                     /translation="MSADLQLISEALAQQNRLLREIANDLRTLVRVAELSHPEFADPT
                     PSRADNTIRPASIAVDPGLLPNGGRR"
     gene            2175..2543
                     /gene="ssrA"
                     /locus_tag="PLQ18_06905"
     tmRNA           2175..2543
                     /gene="ssrA"
                     /locus_tag="PLQ18_06905"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            2818..>3463
                     /locus_tag="PLQ18_06910"
     CDS             2818..>3463
                     /locus_tag="PLQ18_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010640951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HNE01421.1"
                     /translation="MTPETRARLQIDRKLVQAGWIIQDMKQLNLAAGPGVAVREYPTD
                     SGPADYVLFVRRHAAGVIEAKKDSLGEKLTATEPQTERYATASLKWRKDSTPLRFLFE
                     ATGQIIRFTDSADPVPRSREIFHFFKPETLAAWLAQPESLRCRLAGRMPALPEQNLRD
                     CQISAVNGLEKSLALNKPRALVHMATGAGKTFTAITSVYRLLKFGGAKRVLFLVD"
BASE COUNT          516 a         1235 c         1157 g          555 t
ORIGIN      
        1 agatcggccg gcgacagcgc cgcggcgccc tcggcgcgcg cggcatcggc ggccggcgtc
       61 cggccgttcg tggcagccgc ttcggccgca gcggtgaaac ccggcgcgag catcagcatc
      121 agccacagga cggcgaagcg tggatttggc atcgtgtacg tctcccccga ttccgctacg
      181 gcagtctggc tgtgaccttc ctgccgctgt ttttgttgcg acgcaatagt catacgaccc
      241 cggaaacgac gcctcgatga ccgcagagtt cacgctgatc gacgatctga tcgcccggcg
      301 caccggcgcg cagcgggccg atgtggcgct cggcatcggc gatgactgcg cgctgctcgt
      361 gccgccgccg ggccggcagc tcgcggtcag cctcgacctg ctggtcgccg gcgtgcactt
      421 cctgcccgat gccgagccgg tcgcgctcgg ccacaaggcg ctggcggtga acctgtcgga
      481 tctggccgca agcggcgccg aaccggcgtg gatcacgctc ggcctgacgc tgccgggggc
      541 cgatgccggc tggctcgcgg ccttcatgga cggcttcgcc gcgctcgccg ccgagcacgg
      601 cgcgcagctc gtcggcggcg acaccacgcg cgggccgctc tgcatcgccg tgcaggtgca
      661 cggcttcgtg ccgcccggcg cggcgctgac ccgcagcggg gcgcgagcgg gcgatctggt
      721 ctgcatcacc ggcacgcccg gcgaggccgc cgccgggctg gcgctgcgct tcggccggct
      781 ggagctgccc gagcccttcg ccacgcactg tctcgcccgc ctcgaccggc cgacgccgcg
      841 catccgcgcc gggctggcgc tgcgcggact ggcgagcgcc tgcatcgatg tctccgacgg
      901 cctcgcgcag gacctcggcc acatcctctc ggccagcggc tgcggcgcaa cgctcgcgct
      961 cggacgattg ccgctgaccg ccgtcagcgc cgcgctcgga gccgaggccg catggccgta
     1021 tgcgctcgcc ggcggcgatg actacgagct gctgttcacg ctgcccgcgg cgcagcaggc
     1081 cgaagccgaa gcccggctcg ccgccgccgg ctgtccgttc agcgtcatcg gccgcatcga
     1141 tcccgaaccc ggcctgcgcc tcctgcgccc cgatggccgg ccgcatgcgc tggaacgggg
     1201 cggttatgaa cacttcgcct gagcgcgcgc cgcgcgtctt ccgcgactgg cggcatttcc
     1261 tcgccttcgg cctcggcagc ggcctcgtcc cggtcgcgcc gggcacggcc ggcacgctcg
     1321 ccgccgtgcc gttcgcgtgg gggctggccg cgctgtcgcc ggcggcttac ctgttcatcg
     1381 tgctcgcggc cttcgcgctc ggcgtgtacg tctgcgggcg caccagccgc gatctcggcg
     1441 tgcacgatca cggcggcatc gtctgggacg agttcgtcgg ctactggatc acgatgtaca
     1501 tggccccgcc cggccccggc tggctgatcg ccggcttcgc gctgttccgg ctgttcgatg
     1561 tctggaagcc gtggccgatc cggattttcg atgtccacgt gcccggcgga cttggcatca
     1621 tgctcgacga cgtactggct ggactgtacg cctgggtact gctgcaggcc ctgaccgccg
     1681 tgctgtgacc cgcgcgcaac cgaaggtgcg caacaccccg caagccttga tccgtatcga
     1741 cgagtaccca tcagtaccct gccgcgccgc ccgccggcgg ccgtcaggac cacgacctga
     1801 tgcatcagga ggacgcatga gtgccgacct gcagttgatc tccgaagccc tggcccagca
     1861 gaaccgcctg ctgcgcgaga ttgccaacga cctgcgcacg ctggtgcgcg tcgccgagct
     1921 ttcccatccc gagttcgccg atccgacgcc ctcgcgcgca gacaacacca tccgcccggc
     1981 cagcatcgcc gtcgatccgg ggctgctgcc gaacggcggc aggcgctgag gccggaacgc
     2041 gaccggcaca cccggcttga aatccgccgc ggcaccgcca tatcggtcag cggtgtcgcg
     2101 cagtccgcaa gtccgctgcc cggcaccggc gctggcagcg gccttgcggt cggtgtacag
     2161 tcgccctccc ttcaggggcc gacctggttt cgacgtgggt ggcaaaacct gaggcgcatg
     2221 ccgaggacgt cgctaggcct cgtaaaaaaa gcgacaactc atagttgcca acgacgacaa
     2281 ctacgctctg gccgcctaac ccggccagcc tctgaccgga acttgctcgt gaagtccggg
     2341 tgcccgcaag ggcacatcag gggtcgacca gcacgagatc gcgcgcaagc ccgcccgggg
     2401 cccaggcgct aaaaccaagc gggaccgccg ttcgagaccc tgcccgtcgg gacgagaatg
     2461 gtcagatcaa tgactgggct aagcatgtag tgccaagggc ggagcgcttg cggacgcggg
     2521 ttcgattccc gccggctcca ccagaccagc aaggcccgga tgatccatcc gggccttttc
     2581 ttttgggcgt tccttctggc gatattgtcg cgttgcgcgg gatcacggcc ggccgctccc
     2641 gcgggggcga tactgggtgc tgccatgaac gggttatcgt acgccagcgc catttcagcg
     2701 gtggccgggc gatggcaggt ggacgatgtt tcggcgcctg tcagatgaat caaggagttg
     2761 tattgcccct gccctgtcgg tcatgggcaa cgcatacgga gctcgggggg agcacgcatg
     2821 acgccggaaa ccagggcacg cctgcagatt gaccggaagc tggtgcaggc cggctggatc
     2881 attcaggaca tgaaacagct caatctggca gccggcccgg gcgtggccgt gcgcgagtac
     2941 cccaccgaca gcggcccggc cgactacgtg ctgttcgtca ggcgccatgc ggcgggcgtg
     3001 atcgaagcca agaaggacag tctgggcgag aagctgaccg cgacggaacc ccagaccgag
     3061 cgctatgcca ccgccagtct caaatggcgg aaggacagca cgcccctgcg ctttctgttc
     3121 gaggccaccg gtcagatcat ccgctttacc gacagcgccg atcccgtacc gcgctcacgc
     3181 gaaatctttc atttcttcaa gccggaaacc ctggccgcat ggctggcgca gcctgaaagc
     3241 ctgcgctgtc gtctggccgg gcgcatgccg gctctgccgg aacagaatct gcgcgactgt
     3301 cagatcagtg ccgtcaacgg gctggagaaa tcgctggcac tgaacaagcc tcgcgccctc
     3361 gtgcatatgg ccaccggtgc cggcaagacc ttcacggcca tcacctcggt ttatcgcctg
     3421 ctcaagttcg gcggcgcgaa gcgtgtgctg tttctggtgg ata
//
LOCUS       DAOQJH010000259         3453 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_17496_length_3453_cov_4.664509, whole genome shotgun sequence.
ACCESSION   DAOQJH010000259 DAOQJH010000000
VERSION     DAOQJH010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3453)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3453)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3453
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17496_length_3453_cov_4.664509"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..586
                     /locus_tag="PLQ18_06915"
     CDS             <1..586
                     /locus_tag="PLQ18_06915"
                     /inference="COORDINATES: protein motif:HMM:NF016668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HNE01422.1"
                     /translation="LADGRLPGKFAALAAIATGRWPLAEVEAEWDAQIGRVADAGLNP
                     VFINSHEHVHMLPALYRVAARLARRHGIRWLRRTTPEWQGAQGLIRNLLLGAMAAATR
                     ADSPQPRLLGLAGSGRLGLPELRRALSGLAPGGRYELMCHPGEFDAAEVRDGRLLAYH
                     DWSRELAALTDPGFRDHCAQAGIRLVSFRALAAE"
     gene            595..1623
                     /locus_tag="PLQ18_06920"
     CDS             595..1623
                     /locus_tag="PLQ18_06920"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNE01423.1"
                     /translation="MVQLEVVDSSTPRVQPEPPTISVVLPAHNEGEGIRHAIEVVAGV
                     LRQCNVSHELIVVDDGSRDDTWEQIREAAARHGYVRGLRFSRNFGKEAALLAGLRAAR
                     GKAVITMDADLQHPPSAIPEMLKCWREGAMVVHAVKRDRSTDSFMQRARAKLFNGLIS
                     RLGRIDLESASDYKLLDRTAVEVLVERLPERERFFRGLAQWIGFPQASVPFSVAPRAA
                     GTGKWSLLGLVNLATTAVLSFTGAPLRLVTILGSVTLLFAAIVGGEALVSWVRGNAAA
                     GFLTLEFTLLIIGSFIMISLGIVGEYIAKIYEELKGRPSFLVHERAGFEDEAAETGRE
                     REPSRFAG"
     gene            complement(1702..>3453)
                     /locus_tag="PLQ18_06925"
     CDS             complement(1702..>3453)
                     /locus_tag="PLQ18_06925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE01424.1"
                     /translation="PGLAAARITRKVLKDNKGNGGVSGFRIKTLSPVEFTDLGLSDGW
                     GVEGSGLLTIDSLPFLKGAQIGVMLIDGELGFAAIFDAGEFKLGGPLKMTGGNLALAI
                     VPGGPLQASGRIDFALGEYASGSIAAGAGPGGVTLDGSLAFEKGLFSGEGHLRYAAGK
                     WSADGSFTLQRKFPGVDKLSLTLGYDGETQLLRLGGDADLSVPGFRKASCAVAADAAG
                     NIALSGSAELADTLPKIKAGRLTLTLNRPAGGDWSFGGSGELEPDLKGVPASAKLLLS
                     YADGLVTGKLAAAYATKLVGGTLTLNAKAGVGEQPEPLKVWGSGNVDFKAAPWLKATV
                     GLALAEDGSLTVAGKLGIPAPVELFPKKEYKKQILPLAAPSIPIFGFAVAGVNVGVFL
                     NIDGSLDFIASFGPGQLTEASVGITWSPDDEDATELDGKATFVIPAYAGLKLTVDAGL
                     GVGAAVISAIGGLSVWGELGVKASAGLGVDLKWKPGLGIKLNGEAKADAEAVLKLGAG
                     GFIKVVAKIIGTIYEERFPAGEFTLGSGLKIGVTLPVSYEPDKSFELDFSKIDIRTPE
                     ISAGDLVDKIKDQLPSP"
BASE COUNT          460 a         1259 c         1191 g          543 t
ORIGIN      
        1 tctcgccgac ggccgcctgc ccggcaagtt cgcagccctc gcggcgatcg cgaccggccg
       61 ctggccgctc gccgaagtcg aggccgagtg ggatgcgcag atcggccgcg tcgccgatgc
      121 cgggcttaat ccggtcttca tcaacagcca cgagcacgtc catatgctgc cggccctgta
      181 ccgggtcgcg gcccggctcg cgcgccgcca cggcatccgc tggctgcggc gcacgacgcc
      241 cgagtggcag ggcgcgcagg ggctgatccg caacctgctg ctcggcgcga tggccgccgc
      301 cacgcgtgcc gactcgccgc agccgcgcct gctcggtctt gccggcagcg gtcggctcgg
      361 tctgccggag ctgcgccggg cgctgtcagg tctggcgccc ggcggtcgat acgaactgat
      421 gtgccacccc ggcgagttcg atgccgccga ggtgcgcgac gggcggctgc tcgcctatca
      481 cgactggtcg cgcgagctcg ccgcgctgac cgaccccggt ttccgcgatc actgcgcgca
      541 ggccggcatc cgtctggttt ccttccgggc cctcgccgcg gagtgatcgt catcatggtc
      601 cagcttgaag tcgtcgattc gtccaccccg cgcgtgcagc cggagccgcc gaccatcagc
      661 gtcgtgctgc cggcgcacaa cgagggcgag ggcatccgcc acgccatcga ggtcgtcgcc
      721 ggcgtgctgc gccagtgcaa cgtcagccac gagctgatcg tcgtcgacga cggttcgcgc
      781 gacgacacct gggagcagat ccgcgaagcc gccgcccggc acggctacgt gcgcggcctg
      841 cgcttctcgc gcaacttcgg caaggaggcc gcgctgctcg ccggcctgcg tgcggcgcgc
      901 ggcaaggccg tgatcacgat ggatgccgac ctgcagcacc cgccgtcggc gatcccggag
      961 atgctgaagt gctggcgcga gggcgcgatg gtcgtgcacg cggtcaagcg cgaccgctcg
     1021 accgacagct tcatgcagcg tgcgcgcgcg aagctcttca acggcctgat cagccggctc
     1081 gggcgcatcg atctcgaaag cgcgtcggac tacaagctgc tcgatcgcac cgcggtcgag
     1141 gtgctggtcg agcgcctgcc cgagcgcgag cgcttcttcc gcggcctggc gcagtggatc
     1201 ggcttcccgc aggccagcgt gccgttcagc gtcgcgccgc gcgccgccgg caccggcaag
     1261 tggtcgctgc tcggcctcgt caacctcgcc accaccgccg tgctgtcgtt caccggcgcg
     1321 ccgctgcgcc tggtgaccat cctcggctcg gtgacgctgc tgttcgcggc catcgtcggc
     1381 ggcgaggcgc tggtgtcgtg ggtgcgcggc aacgccgcgg ccggcttcct gacgctcgag
     1441 ttcacgctgc tgatcatcgg cagcttcatc atgatcagcc tcggcatcgt cggcgagtac
     1501 atcgccaaga tctacgagga actgaagggc cggccgtcct tcctggtcca cgagcgcgcc
     1561 ggtttcgagg atgaagccgc cgagaccggt cgcgagcgcg agccctcgcg cttcgccggc
     1621 tgatccgcgc gcagacggcc tcccccgcct gtccccgacg gcgcatccgg cccggccggc
     1681 ggcgccgtcg tcgttcctgc ctcacgggct cggcagctgg tccttgatct tgtcgacgag
     1741 atcgccggcg ctgatctcgg gcgtgcggat gtcgatcttc gagaagtcga gctcgaagga
     1801 cttgtccggc tcgtagctga ccggcagcgt cacgccgatc ttcaggcccg agccgagcgt
     1861 gaattcgccg gccgggaagc gctcctcgta gatcgtgccg atgatcttgg cgacgacctt
     1921 gatgaagccg ccggcgccga gcttcagcac cgcctcggca tcggccttgg cctcgccgtt
     1981 gagcttgatg ccgaggccgg gcttccactt caggtcgacg ccgagcccgg cgctggcctt
     2041 aacgcccagt tcgccccaga cgctcaggcc gccgatggcg ctgatcaccg ctgcgccgac
     2101 cccgagtccg gcatcgacgg tcagcttcag cccggcgtag gcggggatca cgaaggtggc
     2161 cttgccgtcg agttcggtgg cgtcctcgtc gtccgggctc caggtgatgc cgaccgaggc
     2221 ctcggtgagc tggccgggcc cgaagctggc gatgaagtcg agcgagccgt cgatgttcag
     2281 gaacaccccg acgttcacgc cggcgaccgc gaagccgaag atcgggatcg agggcgccgc
     2341 gagcggcagg atctgcttct tgtattcctt cttcgggaac agctcgaccg gcgccgggat
     2401 gccgagcttg ccggcaacgg tcagcgagcc gtcctcggcc agcgcgaggc ccaccgtcgc
     2461 cttcagccac ggcgcggcct tgaagtcgac gttgccgctg ccccagacct tcagcggctc
     2521 gggctgctcg ccgacgccgg ccttggcgtt cagggtcagc gtgccgccga cgagcttcgt
     2581 ggcatacgcc gccgcgagct tgccggtgac gaggccgtcg gcgtagctca gcagcagctt
     2641 cgccgaggcc ggcacgccct tgaggtcggg ttcgagttcg ccgctgccgc cgaagctcca
     2701 gtcgccaccg gccgggcggt tcagcgtcag cgtcagccgc ccggccttga tcttcggcag
     2761 cgtgtcggca agctcggcgc tgcccgacag cgcgatgttg ccggcggcat cggcagcgac
     2821 cgcgcagctc gccttcctga agccgggcac gctgagatcc gcatcgccgc cgaggcgcag
     2881 cagctgcgtt tcgccgtcgt agccgagcgt cagcgacagc ttgtcgacgc cggggaactt
     2941 gcgctgcagc gtgaagctgc cgtcggccga ccacttgccg gcggcgtagc gcagatggcc
     3001 ctcgccgctg aacagcccct tctcgaaggc gagcgagccg tcgagcgtga cgccgccggg
     3061 accggcgccg gcggcgatcg agccgctcgc gtactcgccg agcgcgaagt cgatgcggcc
     3121 gctggcctgc aggggcccgc cgggcacgat cgccagcgcc aggttgccgc cggtcatctt
     3181 cagcggcccg ccgagcttga actcgccggc gtcgaagatc gccgcgaagc cgagttcgcc
     3241 gtcgatcagc atcacgccga tctgcgcgcc cttcaggaac ggcaggctgt cgatggtcag
     3301 caaaccgctg ccctcgacgc cccagccgtc gctcaggccg aggtcggtga actcgaccgg
     3361 gctcagcgtc ttgatgcgga agccgctgac gccgccgttg cccttgttgt ccttcagcac
     3421 cttgcgcgtg atgcgcgccg ccgcaagccc cgg
//
LOCUS       DAOQJH010000260         3453 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_17500_length_3453_cov_4.010594, whole genome shotgun sequence.
ACCESSION   DAOQJH010000260 DAOQJH010000000
VERSION     DAOQJH010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3453)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3453)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3453
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17500_length_3453_cov_4.010594"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1057
                     /locus_tag="PLQ18_06930"
     CDS             <1..1057
                     /locus_tag="PLQ18_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007158429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNE01425.1"
                     /translation="DEPTNNLDINTIRWLEDVINARNSTMIIISHDRHFLNSVCTHMA
                     DLDYGELRVYPGNYDDYMTASTQARERLLSDNAKKKAQIAELQTFVSRFSANASKARQ
                     ATSRARQIEKIKLEEVKPSSRVSPYIRFEQYKKLHRQAVTLESVSKGYDGNPPLFKKL
                     SLRIDAGERVAIIGPNGAGKTTLLRCLMQELEPDAGSVKWAEGADIGYYAQDHASDFA
                     DEATLFEWMACWTKGDDQLVRAALGRMLFGNDEIGKSVRVLSGGEQGRMLFGKLTLTR
                     PNVMVLDEPTNHLDMESIEALNLALENYPGTLIFVSHDREFVSSLATRILEFTPRGLV
                     DFTGSYEDYLRSQGIGG"
     gene            1271..3226
                     /locus_tag="PLQ18_06935"
     CDS             1271..3226
                     /locus_tag="PLQ18_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015667173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNE01426.1"
                     /translation="MSGASGSVPVRVLAGDGRGSSFSAADFRFDGQPAALAIAFVSAH
                     LDFAGVCSRLASAAGAVPLLAVSTAGELCSAGGGGGLYRPAAAHGAGVIVQVFAPALF
                     AQVSVQAVALPNDDIRRGAPTLDREARVQRIAGELARVQVPFAIDARDTLALAFVDGL
                     SACENYLMEAVYRSGRFPCLFVGGSAGGKLDFQHTWLYDGRRVLENHALLAFVKMAPG
                     MRYAALKSQNFRDTGRSWVVIDADPDLRRVAAVFDPQRGTVVPFAQALAQSFSVPVER
                     LGDKLNGHTFGIELDGELFVRSVAAIDTGTGALSFFCDVNPGDELKLLAATDFVAQTK
                     RDVQDFLRGKPKPLAVLMNDCVLRRLNNEPRLADLAGLWPVPAAGFSTFGELFGINVN
                     QTLSAVAFFAPGPSPFTDDLVDRFPVHYARFREYFTACRLRRAESLNGLRARVLAHLL
                     GQFEANTALAGEVAEALAQTAGIRSTVEAIRGALLTELATAGDNADVQALAEGFASFE
                     RHTQGFRDVLKTIDRVASQTNLLALNATIEAVRAGEAGRGFAVVATEVKKLANDTRAS
                     LGRTEEVIRSMTAALGELGTHIDSARNRFGALHAHQQGTVGQVETIVANAGLIERALA
                     GLGERAGAQRAAMAELAGDLDLLRRLD"
     gene            complement(3223..>3453)
                     /locus_tag="PLQ18_06940"
     CDS             complement(3223..>3453)
                     /locus_tag="PLQ18_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020654642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNE01427.1"
                     /translation="LVGKLFGVRYLATLFGMTLLSHQIGGFFGAWLGGLSRVGFGDYQ
                     WMWYADIVLALLAALANLPIRENRPLPAAAPA"
BASE COUNT          539 a         1226 c         1190 g          498 t
ORIGIN      
        1 cgacgagccg accaacaacc tcgacatcaa caccatccgc tggctggaag acgtgatcaa
       61 cgcgcgcaac agcacgatga tcatcatttc ccacgaccgc cacttcctga acagcgtctg
      121 cacgcacatg gccgacctcg actacggcga gctgcgcgtc taccccggca actacgacga
      181 ctacatgacc gcctcgacgc aggcgcgcga gcggctgctg tccgacaacg ccaagaagaa
      241 ggcgcagatc gccgagctgc agaccttcgt cagccgcttc tcggccaacg cctcgaaggc
      301 gcggcaggcg acctcgcgcg cgcgccagat cgagaagatc aagcttgagg aggtcaagcc
      361 gtcgagccgc gtcagcccct acatccgctt cgagcagtac aagaagctgc accgccaggc
      421 ggtgacgctc gaatccgtca gcaagggcta cgacggcaat ccgccgctgt tcaaaaagct
      481 gtcgctgcgc atcgacgccg gcgagcgcgt cgcgatcatc ggcccgaacg gcgccggcaa
      541 gaccacgctg ctgcgctgcc tgatgcagga actcgaaccc gacgccgggt cggtcaagtg
      601 ggccgagggc gccgacatcg gctattacgc gcaggaccac gcctcggact tcgccgacga
      661 ggcgacgctg ttcgaatgga tggcgtgctg gaccaagggt gatgaccagc tcgtgcgcgc
      721 cgcgctcggg cgcatgctgt tcggcaacga cgagatcggc aaatcggtca gggtgctctc
      781 gggcggcgag cagggccgca tgctgttcgg caagctgacg ctgacgcggc cgaacgtcat
      841 ggtgctcgac gagccgacca accacctcga catggaatcg atcgaggcgc tgaacctcgc
      901 gctcgagaac taccccggca cgctgatctt cgtcagccac gaccgcgagt tcgtgtcctc
      961 gctggcgacg cgcatcctgg agttcacgcc gcgcggcctc gtcgacttca ccggcagcta
     1021 cgaggactac ctgcgcagtc agggcatcgg cggctgagcc ggcgcgatgg ttttgtgaag
     1081 ccgcatgaaa gcggggctaa agccgcggcg ccggtgacgg atattgacgg atagcacggg
     1141 gatgcgtgcc gggcctgcag caggcgggag ggcggcatcc gcgtcttccg tctgcaagcc
     1201 cgccgaggcc gccatgcctg ccgaagtccg tttcccgtcc gaaagccttc cgctgcccga
     1261 gccgcccgcg atgtccgggg catccgggtc cgtgccggtg cgcgtgctcg ccggcgatgg
     1321 ccggggcagc agcttcagtg ccgccgattt ccgcttcgac ggccagccgg cggcgctggc
     1381 gatcgccttc gtgtcggcgc acctcgactt cgccggcgtc tgctcgcggc tcgcctcggc
     1441 cgccggcgcg gtgccgctgc tcgcggtcag caccgccggc gagctgtgct cggccggtgg
     1501 cggtggcggt ctgtaccggc cggcggctgc gcacggcgcc ggcgtcatcg tgcaggtgtt
     1561 cgcaccggcg ctgttcgcgc aggtcagcgt gcaggcggtg gcgctgccga acgacgacat
     1621 ccgccgcggc gcgccgacgc tcgatcgcga ggcgcgcgtg cagcgcatcg ccggcgaact
     1681 cgcgcgcgtg caggtgccgt tcgcgatcga tgcgcgcgac acgctggcgc tcgccttcgt
     1741 cgacggcctc tcggcctgcg agaactacct gatggaggcc gtctaccgca gcgggcgctt
     1801 cccgtgcctg ttcgtcggcg gctcggccgg cggcaagctc gatttccagc acacctggct
     1861 gtacgacggc cggcgcgtgc tcgaaaacca cgcgctgctc gccttcgtga agatggcgcc
     1921 gggcatgcgc tacgcggcgc tgaagagcca gaacttccgc gacaccggtc gcagctgggt
     1981 ggtcatcgac gccgatccgg acctgcgccg ggtggccgcg gtgttcgatc cgcagcgcgg
     2041 cacggtcgtg ccgttcgcgc aggcgctggc gcagtccttc agcgtgccgg tggagcggct
     2101 cggcgacaag ctcaacggcc acaccttcgg catcgagctc gacggcgagc tgttcgtgcg
     2161 ctcggtcgcg gcgatcgaca ccggcaccgg ggcgctgtcg ttcttctgcg acgtgaatcc
     2221 cggcgacgaa ctgaagctgc tggcggcgac cgatttcgtc gcgcagacga agcgcgacgt
     2281 gcaggacttc ctgcgcggca agccgaagcc gctggccgtg ctgatgaacg actgcgtgct
     2341 gcgccggctc aacaacgagc cgcgcctcgc cgatctcgcc ggtctgtggc cggtgccggc
     2401 ggccgggttc tcgaccttcg gcgagctgtt cggcatcaac gtcaaccaga cgctgagcgc
     2461 ggtggccttc ttcgcaccgg ggccgtcacc gttcacggac gatctcgtcg atcgcttccc
     2521 ggtgcattac gcgcgtttcc gcgagtactt cacggcctgc cggctgcgcc gcgccgagtc
     2581 cctgaacggc ctgcgcgcgc gcgtgctcgc ccatctgctc ggccagttcg aggccaacac
     2641 cgcgctcgcc ggcgaggtcg ccgaggcgct ggcgcagacc gccggcatcc gctcgaccgt
     2701 cgaagccatc cgcggcgcgc tgctgaccga gctggcgacc gccggcgaca acgccgacgt
     2761 gcaggcgctg gccgagggct tcgcgagctt cgagcggcac acgcagggtt tccgcgacgt
     2821 gctgaagacc atcgaccgcg ttgccagcca gaccaacctg ctggcgctca acgcgacgat
     2881 cgaggccgtg cgcgccggcg aggccgggcg cggcttcgcg gtggtcgcga ccgaggtcaa
     2941 gaagctcgcc aacgacacgc gcgcctcgct cgggcgcacc gaggaagtca tccgcagcat
     3001 gacggccgcg ctcggcgaac tcggcaccca catcgactcg gcgcgcaacc gcttcggcgc
     3061 gctgcatgcg caccagcagg gcacggtcgg ccaggtcgag accatcgtcg ccaacgccgg
     3121 cctgatcgag cgcgcgctgg ccgggctcgg cgagcgcgcc ggggcgcagc gcgcggcgat
     3181 ggcggaactc gccggcgacc tcgacctgct gcgccggctc gattaggccg gtgccgccgc
     3241 cggcagcggg cggttctcgc ggatcggcag gttcgcgagt gccgcgagca gcgcgagcac
     3301 gatgtcggcg taccacatcc actggtagtc gccgaaaccg acacggctga ggccgccgag
     3361 ccaggcgccg aagaagccgc cgatctggtg cgacagcagg gtcatgccga acagcgtcgc
     3421 cagatagcgc acgccgaaca gcttgccgac cag
//
LOCUS       DAOQJH010000261         3448 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_17536_length_3448_cov_3.801650, whole genome shotgun sequence.
ACCESSION   DAOQJH010000261 DAOQJH010000000
VERSION     DAOQJH010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3448)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3448)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3448
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17536_length_3448_cov_3.801650"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..216)
                     /locus_tag="PLQ18_06945"
     CDS             complement(<1..216)
                     /locus_tag="PLQ18_06945"
                     /inference="COORDINATES: protein motif:HMM:NF014604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliS"
                     /protein_id="HNE01428.1"
                     /translation="MTPAQQALAQYNRIGTVSAVEDASPHRLIQLLLDAGLERIAHAR
                     RALEAGDVAARGLAIGKALDIVAGLRGS"
     gene            complement(320..2344)
                     /gene="fliD"
                     /locus_tag="PLQ18_06950"
     CDS             complement(320..2344)
                     /gene="fliD"
                     /locus_tag="PLQ18_06950"
                     /inference="COORDINATES: protein motif:HMM:NF018852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HNE01429.1"
                     /translation="MAAISSPGLGSGLDVNNLVSQLMAAERKAPTARLDSASTLNDAR
                     ISALGKITSALDKLQTAAGTLASPNTFQTKTATVSDPSALSVTAARTAAAGSYTVEVS
                     QLARADKLASASFNTAFETVGTGTLTISFGTWNSGSSTFTPNATKPSTSIVIDATNNT
                     LSGIRDAINAAGAGVTASIVGDGSGQRLVFSGSESGAANGLKISVSDNDGNSTDAGGL
                     SQFAFDPAATAGSGRNLTQTVSAQDAKFKLDGLSLTSSTNLVGSAVSGLTLNLRTVTS
                     SPVTVDIARDDSAAAKAVSGLVDAYNEVVSAVKSASSFDSESKKGGPLIGDASVLSVM
                     GRLRGLMTSSIAGLGGVSSLKAVGVSFQKDGTLSFDSSTLADALSSNADSVARLFGAT
                     GKASDSRVSVVVSSSTTKTGNYAVDITTEPAGGLLTGAVTAPGFPLTIDGTNNGLSLL
                     IDGTATGALTLTQGSYASADDLAHELQSRINGASALKTAGAAVTVSWNTDHFEIRSNR
                     YGAASTVSLASGSAATALGLDTGTATAGTDVAGTIGGVAATGSGRILTASGDADGLRL
                     LISGAGTGARGSVDFKRGLGDQLKSLLDDVLADDGTIGRRSSGLSDESARIAKQRTAL
                     DDRMTELETRYRTQFTRLDSVMASLKATSDFLTSQLNAMTNSGSSSSSSN"
     gene            complement(2382..2819)
                     /locus_tag="PLQ18_06955"
     CDS             complement(2382..2819)
                     /locus_tag="PLQ18_06955"
                     /inference="COORDINATES: protein motif:HMM:NF015602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlaG"
                     /protein_id="HNE01430.1"
                     /translation="MTLSIPPAGGAIERPPATAVAVQQAISPGAAQNLTPEAVAPVDP
                     VAAIAALGDVRGRAGNEPQQQGSVEERERELQRATEDVNRYLSESHRALNFSIDRESG
                     ETVVKVMDTEKNEVIRQIPSEEVLALARRLRDGEEGYLVHAQA"
     gene            complement(2918..>3448)
                     /locus_tag="PLQ18_06960"
     CDS             complement(2918..>3448)
                     /locus_tag="PLQ18_06960"
                     /inference="COORDINATES: protein motif:HMM:NF012903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HNE01431.1"
                     /translation="VHLEAADGRNIIHSFTGVTAASTGVGAAATTFGTYTLHSASAID
                     VGAGTTNVVTGVANSGLGVGTYGETKTGSSLKDLDISTVDGANKALTAIDNALKQIDE
                     NRSTLGAVQNRFGSTLNTLATNAENLTAARSRIQDADFASETASLSKNQILQQAGTAM
                     LAQANQLSQGVLSLLR"
BASE COUNT          467 a         1137 c         1234 g          610 t
ORIGIN      
        1 gctgccgcgc aggccggcca cgatgtcgag cgccttgccg atggccaggc cgcgcgcagc
       61 gacatcgccg gcttccagcg cccggcgcgc atgggcgatg cgctcaaggc cggcgtcgag
      121 cagcagctgg atcagccggt gcggcgaggc atcttccacg gcactgacgg taccgatgcg
      181 gttgtactgc gcgagtgcct gctgggcggg ggtcatgtcg ggttcctctg gagtacgtcc
      241 cgcgcgtgcc ggcggggccg tgggtggatt ggcgcggccg cagccgggcc ggtgtgatcg
      301 cctgcgaaca ccgcatccgt cagttcgaac tgctggaact gctgccggag ttggtcattg
      361 cattcagttg cgaggtcagg aagtcgctgg tcgccttcag gctcgccatc accgagtcga
      421 gccgcgtgaa ctgcgtgcgg tagcgggttt cgagttcggt catgcggtcg tcgagggcgg
      481 tacgctgctt cgcgatgcgc gcgctctcgt cactgagacc gctgctgcgc cgcccgatcg
      541 tgccgtcatc ggcgagcacg tcatcgagca gggacttgag ctggtccccg agcccgcgct
      601 tgaagtcgac gctgccgcgc gcgccggtgc cggccccgga gatcagcagc cgcagcccgt
      661 cggcatcgcc gcttgcggtc aggatgcggc cgctgccggt ggcggcaacg ccgccgatgg
      721 tgccggcgac atcggtgccg gccgtggccg tgccggtatc gaggccgagc gcggttgccg
      781 ccgagccgct ggcgagcgac acggtcgagg ccgcgccgta gcggttcgag cggatctcga
      841 agtgatcggt gttccagctc acggtcaccg cggcaccggc ggtcttcagc gccgaggcac
      901 cgttgatgcg gctctgcagc tcgtgcgcga gatcatcggc actcgcatag ctgccctggg
      961 tcagcgtcag cgcaccggtg gcggtgccgt cgatgagcag gctcaggccg ttgttggtgc
     1021 cgtcgatggt cagcgggaaa cccggcgccg tgaccgcgcc ggtcagcagg ccgccggccg
     1081 gctcggtagt gatgtccacc gcgtagttgc cggttttggt cgtcgaggac gacacgacca
     1141 ccgacacgcg gctgtcgctg gccttgccgg tggcaccgaa caggcgggcg acggaatcgg
     1201 cattgctgct cagggcatcg gcgagcgtgc tcgaatcaaa gctcagcgtg ccgtccttct
     1261 ggaaggacac gccgacggcc ttcaggctcg acacaccgcc caggccggcg atcgaggaag
     1321 tcatcaggcc gcgcagccgc cccatgaccg acagcaccga ggcatcgccg atcagcggcc
     1381 cgcccttctt cgattcactg tcgaagctgg aggccgactt cacggccgac accacttcgt
     1441 tgtaggcatc gaccagaccc gacaccgcct tggccgcggc gctgtcgtcg cgggcaatgt
     1501 cgacggtgac cgggctgctg gtcacggtcc tgaggttcag cgtcaggccc gacaccgccg
     1561 agccgaccag attcgtgctg ctggtcagcg acaggccatc gagcttgaac ttggcgtcct
     1621 gggcgctgac ggtctgcgtc agattcctgc cgctgccggc ggtcgcggcc ggatcgaagg
     1681 cgaactgcga cagcccgccg gcatcggtcg aattgccgtc gttgtcgctg acgctgatct
     1741 tcagcccgtt cgcggccccc gactcgctgc ccgagaacac caggcgctgg ccgctgccat
     1801 cgccgacgat gctggccgtg acgccggcac cggcggcgtt gatcgcatcg cggatgccgg
     1861 agagcgtgtt gttggtggca tcgatgacga tgctggtcga gggcttggtg gcgttcggcg
     1921 tgaaggtgct gctgcccgag ttccaggtgc cgaacgagat cgtcagcgtg ccggtaccga
     1981 cggtctcgaa tgccgtgttg aagctggcac ttgcgagctt gtcggcgcgc gcgagctgcg
     2041 agacctcgac cgtgtagctg ccggcggcgg cggtgcgcgc ggcggtcacc gacagcgccg
     2101 acggatcgct gacggtcgcc gtcttggtct ggaaggtgtt cgggctcgcc agcgtgccgg
     2161 cggcggtctg cagcttgtcg agcgccgacg tgatcttgcc gagcgccgag atgcgcgcat
     2221 cgttcagcgt gctggcgctg tcgaggcgcg ccgtcggcgc cttgcgttcc gccgccatca
     2281 gctgcgagac gaggttgttc acgtcgagtc cggagccgag tccgggggag gatatggctg
     2341 ccatggcgct tgtgctctcg ggcaaaggtg acggaaatcc gtcaggcctg ggcgtgtacg
     2401 aggtatccct cctccccatc acgcaagcgt cgtgccagcg ccaggacttc ttcggacggg
     2461 atctggcgga tgacctcgtt cttctcggta tccatgacct tgacgacggt ctcgccgctt
     2521 tccctgtcga tgctgaaatt cagcgcgcgg tgcgactcgg acagatagcg gttgacgtcc
     2581 tcggtcgcgc gctgcagttc gcgctcgcgc tcctcgacgc tgccctgctg ctgcggttcg
     2641 ttgccggcac ggccgcgcac gtcaccgagc gcggcgatcg ccgccaccgg atcgaccggt
     2701 gcgaccgcct ccggcgtcag gttctgggcc gcgcccggcg agatcgcctg ctgtaccgcg
     2761 acggccgtcg ccggcgggcg ctcgatcgcg ccgccagccg gcggaatgct caaggtcatg
     2821 atccacctcc cgaaagccca aaggcccggg ggcgtactct ccgcccccgg gccgttttca
     2881 acgggcgctc atgcgcctcc tgtcagccgg tctgagctta gcggagcagg ctcagcacgc
     2941 cctgcgagag ctggttggcc tgcgcgagca tcgcggtgcc ggcctgctgc aggatctggt
     3001 tcttgctgag cgaggccgtt tccgaggcga agtcggcatc ctggatgcgc gaacgggcgg
     3061 cggtcaggtt ctcggcgttg gtggcgagcg tgttcagcgt cgagccgaag cggttctgca
     3121 cggcaccgag cgtcgagcgg ttctcgtcga tctgcttgag cgcgttgtca atcgccgtca
     3181 gcgccttgtt ggctccgtcc accgtcgaga tgtcgagatc cttcagcgag ctgccggtct
     3241 tggtttcgcc gtaggtgccg acaccgaggc cggagttcgc aacaccggtg acgacgttgg
     3301 tcgtgccggc accgacgtcg atcgcgctcg ccgagtgcag cgtgtaggtg ccgaaggtgg
     3361 tcgccgcagc gccgacgccg gtgctggccg cggtcacgcc cgtgaacgag tggatgatgt
     3421 tgcggccatc cgcggcttcg aggtgcac
//
LOCUS       DAOQJH010000262         3439 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_17610_length_3439_cov_4.072400, whole genome shotgun sequence.
ACCESSION   DAOQJH010000262 DAOQJH010000000
VERSION     DAOQJH010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3439)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3439)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3439
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17610_length_3439_cov_4.072400"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..229)
                     /locus_tag="PLQ18_06965"
     CDS             complement(<1..229)
                     /locus_tag="PLQ18_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HNE01432.1"
                     /translation="MLKSLLRALMRTLFRVEIRGRTDVFGHARTLIVANHESFLDGLL
                     LGLFLPVEPVFVVHTQVAVHPVFGRILRHIEH"
     gene            complement(429..749)
                     /locus_tag="PLQ18_06970"
     CDS             complement(429..749)
                     /locus_tag="PLQ18_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HNE01433.1"
                     /translation="MILSTTPTLEGHRIREYRGIVSAEAILGANVVKDWFAGLTDFIG
                     GRSGTYEGVLRDAREEAFAELTRAAQRLGANAVVGIDIDYEVLGEQNGMMMVAVSGTA
                     VVID"
     gene            complement(1168..1521)
                     /locus_tag="PLQ18_06975"
     CDS             complement(1168..1521)
                     /locus_tag="PLQ18_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015260575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HNE01434.1"
                     /translation="MNRTPRTENLTRLSSEEARQRIARGESESDWARVNAIPDDEIGA
                     DDPDALPMTDEELATAPILRRPGQRGLQKLPTKQRTTLRLSADVLAHFRADGAGWQTR
                     INDALKQWIAEHGER"
     gene            complement(1499..1780)
                     /locus_tag="PLQ18_06980"
     CDS             complement(1499..1780)
                     /locus_tag="PLQ18_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010643055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HNE01435.1"
                     /translation="MRYEWDEAKRQQNLVKHGLDFVHAVAVLEHPDLLLIADNRRDYG
                     ETRLIGFGPLTGRIHVVVHVARTAGCIRIVSFRKANHREVRFYEQNAQN"
     gene            complement(1873..2550)
                     /locus_tag="PLQ18_06985"
     CDS             complement(1873..2550)
                     /locus_tag="PLQ18_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007178512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016706 - 2-oxoglutarate-dependent
                     dioxygenase activity [Evidence IEA];
                     GO_function: GO:0031418 - L-ascorbic acid binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-dependent dioxygenase"
                     /protein_id="HNE01436.1"
                     /translation="MLLHIPEVLPRDRLAGFRERLARADWLDGRRSAGHQALAVKHNQ
                     QLADDDPLARELAAQALAALERNPLFLTATLPARIFPPMINRYAGGGSYGNHVDNAVR
                     VLPDGTRVRTDVSATLFLSEPEDYDGGELTIDDTYGTHTVKLPAGDLVIYPSTSLHRV
                     TPVTRGARIACVFWVQSLIRDDAQRTLLLDLDLAIQRLNRSGAEPDVAVALGGTYHNL
                     LRMWATP"
     gene            complement(2654..>3439)
                     /locus_tag="PLQ18_06990"
     CDS             complement(2654..>3439)
                     /locus_tag="PLQ18_06990"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNE01437.1"
                     /translation="ASSLAETFTPATGTRLTRDDRKRTDTMTSGRIGVIWQPSPTQSY
                     YAAFGTSFNPTAETLTISSNNAGLDPEENHNFEIGAKLDFLDGALSVRTALFRTEKIN
                     ARTLDPDQLNVNVLDGRWVTDGIELDVIGRITPQWSVFAGLAFMDPEITESNNPAEVG
                     KRPANAPRHTLSVWTTYDLGGGWEIGGGVQGAGKRYGSNTNTNYVPGYTRWDAEVAYK
                     QPDFDVRLNVVNLTDTQWYEAVYTGHAIPGTGRAATLSVDYRF"
BASE COUNT          511 a         1090 c         1230 g          608 t
ORIGIN      
        1 gatgctcgat gtggcgcagg atgcggccga agaccggatg cacggcgacc tgcgtgtgca
       61 cgacgaacac cggctcgacc ggcaggaaca ggccgagcag caggccgtcg aggaaggatt
      121 cgtggttggc gacgatcagc gtgcgtgcat gcccgaacac atcggtacgg ccgcggatct
      181 cgacgcggaa cagcgtgcgc atcagcgcgc gcaacaggga tttcagcatc tgcgggactc
      241 ccgctccgcc cgcggccccg cgggggaggg tgttcttgtc agggaaagcc ggcgccgctg
      301 cgcgggcgcc aggcgatgcg cgtccggtct tcggcccgcg gacgggcggg accgcgcggg
      361 ctcggccctt ccccgcgcgg gaaaagcccc ggtcagaagg aaaagcggtc ttcggcggcc
      421 gcgcgcgttc agtcgatgac gaccgccgtg ccgctgacgg cgaccatcat catgccgttc
      481 tgctcgccga gcacttcata gtcgatgtcg atgccgacca cggcattcgc cccgaggcgc
      541 tgggccgccc tggtcagctc ggcaaacgct tcctcgcgcg catcgcgcag cacaccttcg
      601 taggtgccgc tgcgcccgcc gatgaagtcg gtgaggccgg cgaaccagtc cttgacgaca
      661 ttggccccga ggatggcctc ggcgctgacg atgccgcggt actcgcggat gcggtggcct
      721 tcgagcgtcg gtgtggtgct caggatcatc ggcgtggcct cgtgggcggt ggggacgggg
      781 agggtatagc cgaagaagcg gcgtcgctgc gaacgcaggg cggtgtcgat gcgggaagcc
      841 cggcaggcgt gcgtccctgc gcggcatcgg cacgtccacg cgcgtcatct gcggacccat
      901 gcgcggcatc ggcacttcga tgcgcgccat gcgtgatccg ctgcccggcg tggcgcgacg
      961 gtggtcgcgc atgaaaaacc ggggcggcaa gccccggtcg tggatgggcg gtatcagggc
     1021 cgatcggcgg gcggtatgca cccggggcag gcgaagcgcc tgctcatccg tgtcgaccgt
     1081 tcgtctttcg ggatgcacgc gctgatgccg ggcaactggc gtgtcgcgga agaccggtgg
     1141 cggctacggg ctgctcgcga agcatcgtca gcgttcaccg tgttcggcaa tccattgctt
     1201 cagggcatcg ttgatccggg tctgccagcc cgccccatcc gcccggaagt gcgcaagcac
     1261 atcggctgac aggcgcaggg tcgtgcgttg cttcgtcggc agtttctgta atccgcgctg
     1321 gcccggccgg cgcaggatcg gtgcagtcgc gagttcttcg tccgtcatcg gcagcgcatc
     1381 cggatcgtcg gcaccgatct cgtcgtccgg gatcgcgttg acccgcgccc aatcgctttc
     1441 cgattctcca cgggcaatgc gctgacgggc ttcctcgctg ctcagtcgtg tcagattttc
     1501 agttctgggc gttctgttca taaaaacgca cctcccgatg gttggccttc cggaacgaca
     1561 cgatgcggat gcagccggca gtgcgcgcga cgtgtacgac aacatgaatg cgtcctgtca
     1621 gcggcccgaa tccgatcagg cgggtttcac cgtaatcacg tcgattgtca gcaatcagca
     1681 gcagatcagg gtgttccagt accgctacgg catggacgaa atccagtcca tgcttgacca
     1741 gattctgttg acgcttggcc tcatcccatt cgtagcgcat tgcttattgt aggtacaaaa
     1801 agaccgccaa ggcagggtcc tgctgctgct tcgctgattc cgtcgcctcc gtgcttccct
     1861 tcctcagcct cctcacggcg tcgcccacat cctcagcagg ttgtggtacg tcccgccgag
     1921 cgccaccgcc acgtccggtt ccgcgccgct gcgattcagg cgctggatcg cgaggtccag
     1981 gtcgagcagc agcgtgcgct gcgcatcgtc gcggatcagg ctctgcaccc agaacacgca
     2041 ggcgatgcgc gcgccgcgcg tgaccggtgt cacgcggtgc aggctggtcg acgggtagat
     2101 caccagatcg ccggccggca gcttgacggt gtgcgtgccg taggtgtcgt cgatggtcag
     2161 ttcgccgccg tcgtagtcct ccggctcgct gaggaacagc gtcgccgaga cgtcggtgcg
     2221 cacgcgcgtg ccgtcgggca gcacgcgcac ggcgttgtcg acgtggttgc cgtagctgcc
     2281 gccgccggcg tagcggttga tcatcggcgg gaagatgcgt gccggcagcg tcgcggtcag
     2341 gaacagcggg ttgcgctcca gcgccgccag cgcctgcgcg gcgagttcgc gggcgagcgg
     2401 atcgtcgtcg gccagttgct ggttgtgctt gacggcgagc gcctggtgac cggcgctgcg
     2461 ccggccgtcg agccagtcgg cgcgggcgag acgttcgcga aagccggcga gccggtcgcg
     2521 gggcaggact tcggggatgt gcagcagcat cgggacgggt ccgggcaggc ggaaacgggg
     2581 cgggcttgaa gcccctctcc cgcgagggga gaggggaggg tcgagacgcg ctttatcgct
     2641 ttattaagga gagtcagaac cggtaatcga cgctcagcgt cgcggcacgg ccggtgccgg
     2701 ggatggcgtg gccggtgtag acggcctcgt accactgcgt gtcggtcagg ttgacgacgt
     2761 tcaggcgcac gtcgaaatcc ggctgcttgt aggcgacttc ggcatcccag cgcgtgtagc
     2821 cgggcacgta gttggtgttg gtgttgctgc cgtagcgctt gccggccccc tgcacgccgc
     2881 cgccgatctc ccagccgccg ccgaggtcgt aggtcgtcca gacgctcagc gtatggcgcg
     2941 gcgcattggc cgggcgcttg ccgacttcgg ccggattgtt cgactcggtg atctccgggt
     3001 ccatgaacgc cagaccggcg aacacgctcc actgcggcgt gatgcgaccg atcacgtcga
     3061 gctcgatgcc gtcagtgacc cagcggccgt cgagcacgtt gacgttgagc tgatccggat
     3121 cgagcgtgcg ggcgttgatc ttctcggtgc ggaacagcgc cgtgcgcacc gacagcgcac
     3181 cgtcgaggaa gtcgagcttg gcgccgatct cgaagttgtg gttctcctcc ggatcgaggc
     3241 cggcgttgtt gctcgaaatc gtcagcgtct cggcggtcgg attgaacgag gtgccgaagg
     3301 cggcgtagta ggactgcgtc gggctcggct gccagatcac gccgatgcgg ccgctggtca
     3361 tcgtgtcggt gcgcttgcga tcgtcgcggg tcaggcgcgt gccggtcgcc ggggtgaagg
     3421 tctcggcgag cgagctggc
//
LOCUS       DAOQJH010000263         3432 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_17663_length_3432_cov_3.996447, whole genome shotgun sequence.
ACCESSION   DAOQJH010000263 DAOQJH010000000
VERSION     DAOQJH010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3432)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3432)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3432
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17663_length_3432_cov_3.996447"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..818
                     /locus_tag="PLQ18_06995"
     CDS             <1..818
                     /locus_tag="PLQ18_06995"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014248690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNE01438.1"
                     /translation="ATVTVHNRSRAPVEALAAKGLRTADSAGTAAAAGDFVILMLTDT
                     PAVDAVLEGPDGVLAGLRPGALVIDMGSTSPLATREFAARVAEAGGEWLDAPVSGGVV
                     GAEAATLSIMAGGSIEALERTRPLLERLGARLTHVGPSGCGQIAKTVNQMIVGLTIGA
                     VAEALALAGRAGADPARVRAALVGGFADSRILELHGLRMIDGDFTAGGRVSTQHKDLL
                     QALELAGAVGLELPATELNRRLYAECIAAGDGGLDHSALYRHYTRTATTGGEG"
     gene            824..1051
                     /locus_tag="PLQ18_07000"
     CDS             824..1051
                     /locus_tag="PLQ18_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013715677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2970 domain-containing protein"
                     /protein_id="HNE01439.1"
                     /translation="MSRDDETPTGRPLRLHELAASALWALLGVQKEANRRRDFARGRP
                     GQFVLIGVIATVLFVLLLIGLVRLALGLAGA"
     gene            complement(1054..1806)
                     /locus_tag="PLQ18_07005"
     CDS             complement(1054..1806)
                     /locus_tag="PLQ18_07005"
                     /inference="COORDINATES: protein motif:HMM:NF016214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HNE01440.1"
                     /translation="MADFRTHLIVGTAASGIGAGALLSIGLLSAAHAPACFALGAIGA
                     LLPDVDLDHSIPARVAFSLVALAGAFALVLALAPRWSLAELALAGAAAWVVLRHGVFG
                     AINRWTVHRGLVHSLPAAAAFALATVLAAQRLYGLPVEAAWLAGGFLGAGFVVHLLLD
                     EIYSVDLLGRRLKRSFGTALSFGDPRNLPGTLALYALAGGLYWLCPPAGGLGQRLLDA
                     GLYRQLGARLLPAHGWFEGLPGLALEGLRRLL"
     gene            complement(1904..2974)
                     /locus_tag="PLQ18_07010"
     CDS             complement(1904..2974)
                     /locus_tag="PLQ18_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007041986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2333 family protein"
                     /protein_id="HNE01441.1"
                     /translation="MSKTDSYDGKPRSLLSVLIHAYHPRTWRERGILYAAGVLLATYV
                     IIVGVLWLIWDSKPSLFEPREIALAMAGGDEKKLVAGYVTTATVIQVADTLLEKPGGY
                     LSNDRMPPGVFMDNVPNWEFGVLTAIRDISRSLRNDFSRAQTQSREDVDLQVAEPKFN
                     FDNNSWLLPPSESEYRDGIRALRRYLDRLADERRDDGQFFTRADNLRDYFSVVEKRLG
                     SLSQRLSASVGHIRVNTNLAGDPNARQSTPTPQEQLIKTSWLEIDDHFYEARGYCWAM
                     IEFLRAIEIDFKDVLANKNAQVSLRQIIHELEATQEPVWSPVILNGSGFALFANHSLV
                     MASYIARANAALIDLRNLLQQG"
     gene            complement(3174..>3432)
                     /locus_tag="PLQ18_07015"
     CDS             complement(3174..>3432)
                     /locus_tag="PLQ18_07015"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011440293.1"
                     /note="catalyzes the formation of malate from glyoxylate
                     and acetyl-CoA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HNE01442.1"
                     /translation="ISSQHIASWLHHGVVGREQVMETFRRMALVVDRQNAGDPLYRPM
                     APAYDGIAFQAALDLVFLGRETPNGYTEPHLHKRRRELKGS"
BASE COUNT          501 a         1172 c         1277 g          482 t
ORIGIN      
        1 gtgcgaccgt caccgtccac aaccgctcgc gcgcgccggt cgaggcgctg gccgccaagg
       61 gcctgcgcac cgccgacagc gccggcacgg cggctgcggc cggcgatttc gtgatcctga
      121 tgctgaccga cacgccggcg gtcgacgcgg tgctcgaagg tcccgacggc gtgctcgccg
      181 gcctgcgccc cggcgcgctg gtcatcgaca tgggttcgac ctcgccgctc gcgacgcgcg
      241 agttcgcggc gcgcgtcgcc gaggcgggcg gcgagtggct ggatgcgccg gtctcgggcg
      301 gcgtggtcgg ggccgaggcg gcgacgctca gcatcatggc cggcggcagc atcgaggcgc
      361 tcgaacggac ccggccgctg ctcgaacggc tcggtgcccg gctcacgcac gtcggcccga
      421 gcggctgcgg ccagatcgcc aagaccgtca accagatgat cgtcgggctg accatcggcg
      481 ccgtcgccga ggcgctcgcg cttgccgggc gcgccggcgc cgatccggcg cgcgtgcgcg
      541 cagccctcgt cggtggcttt gcggattcac gcatcctcga actgcacggc ctgcgcatga
      601 tcgacggtga cttcacggcg ggcgggcgcg tcagcaccca gcacaaggat ctgctgcagg
      661 cgctggaact cgccggggcc gtcgggctgg agctgccggc gacggaactg aaccgccggc
      721 tgtacgccga gtgcatcgcc gccggcgacg gcgggctcga tcacagcgcg ctgtatcgcc
      781 actacacgcg caccgccacc accggtggcg agggctgaag ccgatgtccc gcgacgacga
      841 aaccccgacc gggcggccgc tgcgcctgca cgaactggcg gcgagcgcgc tgtgggcgct
      901 gctcggcgtg cagaaggaag ccaaccgccg gcgcgacttc gcgcgcggcc ggcccgggca
      961 gttcgtgctg atcggcgtga tcgcgaccgt gctgttcgtg ctgctgctga tcgggctggt
     1021 gcggctcgcg ctcggtctgg ccggcgcctg aagctacagc agccggcgca ggccctcgag
     1081 cgccagcccc ggcagccctt cgaaccagcc gtgcgccggc agcagccggg cgccgagctg
     1141 gcggtacagc ccggcatcga gcaggcgctg accgagtccg ccggccggcg ggcagagcca
     1201 gtacaggccg ccggccagcg cgtacagcgc cagcgtgccg gggaggttgc gcggatcgcc
     1261 gaagctcagc gccgtgccga acgagcgttt caggcgccgg ccgagcagat cgacgctgta
     1321 gatctcgtcg agcagcagat gcacgacgaa gcccgcgccg aggaagcccc cggccagcca
     1381 ggccgcttcg accggcaggc cgtacaggcg ctgcgcggcc agcacggtcg cgagcgcgaa
     1441 ggccgctgcg gccggcagcg aatgcacgag gccgcgatgc acggtccagc ggttgatcgc
     1501 cccgaacacg ccatggcgca gcacgaccca ggcagcggca ccggccagcg ccagctcggc
     1561 cagtgaccag cgcggcgcca gcgccagcac cagcgcgaag gcgccggcca gcgcgaccag
     1621 gctgaaggcc acgcgcgccg ggatcgaatg atcgagatcg acatccggca gcagcgcgcc
     1681 gatcgcaccg agcgcgaagc aggccggggc gtgggccgcc gacagcaggc cgatgctgag
     1741 cagcgcgccg gcgccgatgc cgcttgcagc ggtgccgacg atcaggtggg tgcggaagtc
     1801 ggccaatttc agtgattcct cgcaggccgg caaggcagcg ggcgcggacc tcgcggtccg
     1861 cgcccggcgg ttttcggcag tgggtcggga tgaaaccgtc gcgtcagccc tgctgcagca
     1921 ggttgcgcag gtcgatcagc gcggcgttgg cgcgggcgat gtacgaggcc atgaccagcg
     1981 agtggttggc gaacagcgca aagcccgagc cgttcaggat caccggcgac cacaccggct
     2041 cctgcgtggc ttcgagttcg tggatgatct ggcgcagcga gacctgggcg ttcttgttgg
     2101 cgagcacgtc cttgaagtcg atctcgatcg cgcgcaggaa ttcgatcatc gcccagcagt
     2161 agccgcgcgc ctcgtagaag tggtcatcga tctccagcca gctggtcttg atcagctgct
     2221 cctgcggcgt cggcgtcgac tggcgggcgt tcgggtcgcc ggcgaggttg gtgttgacgc
     2281 ggatgtgccc gacgctggcc gacagccgct gcgacagcga gccgaggcgc ttctcgacca
     2341 ccgagaagta gtcgcgcaga ttgtcggcgc gcgtgaagaa ctggccgtcg tcgcgacgtt
     2401 cgtcggcgag ccggtcgagg tagcgacgca gcgcccggat gccgtcgcgg tactcggatt
     2461 ccgacggcgg cagcagccag gagttgttgt cgaagttgaa cttcggctcg gccacctgca
     2521 ggtcgacatc ctcgcgcgac tgcgtctgcg cgcggctgaa gtcgttgcgc agcgaccgcg
     2581 agatgtcgcg gatcgcggtc agcacgccga actcccagtt cggcacgtta tccatgaaca
     2641 cgcccggcgg catgcggtcg ttgctgagat aaccaccggg cttctcaagc agcgtgtcgg
     2701 cgacctggat cacggttgcc gtggtcacgt agccggcgac gagcttcttc tcgtcgccgc
     2761 cggccatcgc cagcgcgatc tcgcgcggtt cgaacagcga cggcttgctg tcccagatca
     2821 gccacagcac gccgacgatg atgacgtagg tcgccagcag cacgccggcg gcgtacagga
     2881 tgccccgctc gcgccaggtg cgcgggtggt aggcgtggat cagcaccgac agcagcgagc
     2941 gtggcttgcc gtcgtaggaa tcggtcttgc tcatggatga gttccctgcc ttgtgcgttc
     3001 ttgtcgcagc cgttgcgacc gcgtcggcag ctcgggagtt tctggtgccg ggcggtccgg
     3061 ccgcttcggg atgggccggc gcgcgggccg gcgtcgacga aaaagatagc ccggccgggc
     3121 gggagccgcg gccgggcgtg cgggagcgtc gtgcaggcgg ggcgtcgccg ggctcagctg
     3181 cccttcagct cgcgccggcg cttgtgcagg tgcggctcgg tgtagccgtt cggtgtctcg
     3241 cggccgagga acaccagatc gagcgcggcc tggaaggcga tgccgtcata ggccggcgcc
     3301 atcggccggt acagcgggtc gccggcgttc tggcggtcga cgaccagcgc catgcgccgg
     3361 aaggtctcca tcacctgctc gcggccgacg acgccgtggt gcagccagct ggcgatgtgc
     3421 tggctggaga tg
//
LOCUS       DAOQJH010000264         3425 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_17727_length_3425_cov_3.090208, whole genome shotgun sequence.
ACCESSION   DAOQJH010000264 DAOQJH010000000
VERSION     DAOQJH010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3425)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3425)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3425
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17727_length_3425_cov_3.090208"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3043
                     /locus_tag="PLQ18_07020"
     CDS             <1..3043
                     /locus_tag="PLQ18_07020"
                     /inference="COORDINATES: protein motif:HMM:NF012926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HNE01443.1"
                     /translation="VRDNVYSILCVWGCGLALRRHRGGDPRAYRFEQGAVKTMRGLLT
                     AMMKQAHKVERFKHTLAPIDALHAKYGSRTGEPVVADDGWGHLQIDATSVFLLMLVQM
                     IASGLRVVYSLDEVDFVQALAHYIARAYRTPDYGIWERGDKINTGDPEINASSVGMAK
                     AALEALRGFDLFGAEGGPASVIHVSSDDIARMRNLLEATLPRESRSKEVDAALLSVIG
                     FPAWAVEDAELVERTRHEVVAKLQGRYGCKRFLRDGHQTVLEDHTRLHYESGELARFE
                     RIESEWPLFFTYLMLDGLMRGRDGQAHEYRERLAPLLVDRGAGPLLPELYLVPEKSVA
                     AEKARPGSQPRLPNANVPLYWAQSLYWLALLIDEGWLHPGDIDPLNRRHRIGRRRECT
                     VQVALIADTAAVETALLDAGVRAQTPEAVLPVRVRQASELVEAMTNVGRNAALGLSGR
                     PRRGVGTLVTCQVFTIGGETVVFLPQFLERQDFYLNLSNRVLISRCKAEFAHVRRHWD
                     QAGQPVFALRLTARMLATDGAGELLDFLRALCAGSCEGLPVRVRPLSELIATAGRKRF
                     DRLRDYHFEFAPLEQERSAGNLLDIDPAAARELDAADLQRLEDLPPPAVPVRLAATRN
                     LHEVIALLEIAARRDGIDAPLPDGRSVRACLEAAYALAAEAGHWGLLRRCAGLLDKHD
                     PKLEDAVANIVAHRKQIALGRGYSDGSVVSTALGNREIVACLRQYAGDDPAGRMLVQE
                     VVLAVETLLKTDPAVFRDTMTIRAWPLVLLVVGDYAWEHRLSQPEAFRRVQTLGPWAF
                     MGRVQAVVRGDNPVSRLARLESLRRDDAAHGLAVPDPQEDAGAEAVVDWLAWRRRHGV
                     LTQLSAHFYEQVWAVLAHCEGLVLGERLDIGNCLDSARLRADMTAAETNFALTVNRLL
                     DKIQSPEYRQLNIEALAALAHLCSANPGLHVAGHIVIDVLTGHAVRLHWLQQHPDHAG
                     HYEAFRAEAWSALYASPPAEVESALIGAFAHLLGEARPAAA"
     gene            3069..3404
                     /locus_tag="PLQ18_07025"
     CDS             3069..3404
                     /locus_tag="PLQ18_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009914330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding protein"
                     /protein_id="HNE01444.1"
                     /translation="MNIGAAARASGVSERMIRHYEKLGLLAPAARTAAGYRTYTAGDV
                     HVLRFVRRARDLGFPVADIARLLALWQDRSRASAEVKRLAAGHIAELDARIASLSAMR
                     DALARLAGA"
BASE COUNT          465 a         1263 c         1224 g          473 t
ORIGIN      
        1 ggtgcgcgac aacgtctatt cgatcctgtg cgtgtggggc tgcggcctgg cgctgcgccg
       61 ccaccgcggc ggcgatccgc gcgcctaccg cttcgagcag ggcgcggtga agacgatgcg
      121 cggcctgctg accgcgatga tgaagcaggc gcacaaggtc gagcgcttca agcacacgct
      181 ggcgccgatc gacgcgctgc acgcgaagta cggcagccgc accggcgagc cggtggtcgc
      241 cgatgacggc tggggccatc tgcagatcga cgcgacctcg gtgttcctgc tgatgctggt
      301 gcagatgatc gcctcgggcc tgcgcgtcgt gtattcgctc gacgaggtcg acttcgtgca
      361 ggcgctcgcg cactacatcg cgcgcgccta ccgcacgccg gactacggca tctgggagcg
      421 cggcgacaag atcaacaccg gcgacccgga gatcaacgcc agctcggtcg gcatggccaa
      481 ggccgcgctg gaggcgctgc gcggcttcga cctgttcggc gccgagggcg gtccggcctc
      541 ggtgatccac gtgtcctccg acgacatcgc gcgcatgcgc aacctgctcg aagccacgtt
      601 gccgcgcgag tcgcgctcga aggaggtcga tgcggcgctg ctgagcgtca tcggctttcc
      661 ggcctgggcg gtcgaggacg ccgaactggt cgagcgcacc cgccacgagg tggtcgccaa
      721 gctgcagggc cgctacggct gcaagcgctt cctgcgcgac gggcaccaga ccgtgctgga
      781 ggatcacacg cgcctgcact acgagagcgg cgagctggcg cgcttcgagc gcatcgagtc
      841 cgaatggccg ctgttcttca cctacctgat gctcgacggc ctgatgcgcg ggcgcgacgg
      901 tcaggcgcac gaataccgcg agcgcctggc gccgctgctg gtcgatcgcg gcgccgggcc
      961 gctgctgccg gagctgtacc tggtgccgga aaaatccgtc gccgccgaga aggcgcggcc
     1021 cggctcgcag ccgcggctgc cgaacgccaa cgtgccgctg tactgggcgc agagcctgta
     1081 ctggctggcg ctgctgatcg acgagggctg gctgcatccc ggcgacatcg acccgctgaa
     1141 ccgccgccac cgcatcggcc ggcggcgcga atgcacggtg caggtggcgc tgatcgccga
     1201 cacggcggcg gtcgagaccg cgctgctcga tgccggcgtg cgcgcgcaga cgcccgaggc
     1261 cgtgctgccg gtgcgcgtgc gtcaggcgag cgagctggtc gaggccatga ccaacgtcgg
     1321 gcgcaacgcg gcgctcggtc tctccggccg gccgcggcgc ggcgtcggca cgctggtcac
     1381 ctgccaggtg ttcacgatcg gcggcgagac ggtcgtgttc ctgccgcagt tcctcgaacg
     1441 ccaggacttc tacctgaacc tcagcaaccg cgtgctgatc agccgctgca aggccgagtt
     1501 cgcgcacgtg cgccggcact gggaccaggc cggccagccg gtgttcgcgc tgcgcctgac
     1561 cgcgcgcatg ctcgccaccg atggcgccgg ggagctgctc gacttcctgc gcgcgctctg
     1621 cgccggcagc tgcgagggcc tgccggtgcg cgtgcgcccg ctgtcggagc tgatcgccac
     1681 cgccgggcgc aagcgtttcg accgcctgcg cgactaccac ttcgagttcg cgccgctcga
     1741 acaggagcgc agcgccggca acctgctcga catcgacccg gccgccgccc gcgaactcga
     1801 cgccgccgac ctgcagcggc tcgaagacct gccgccgccg gcggtgccgg tgcgccttgc
     1861 cgcgacgcgc aacctgcacg aggtcatcgc gctgctggag atcgccgccc ggcgcgacgg
     1921 catcgacgcg ccgctgccgg acgggcgcag cgtgcgcgcc tgtctggagg ccgcgtacgc
     1981 gctcgccgcc gaggccggcc actgggggct gctgcgccgc tgcgccgggc tgctcgacaa
     2041 gcacgatccg aagctcgagg acgcggtcgc caacatcgtc gcgcaccgca agcagatcgc
     2101 gctcgggcgc ggctacagcg atggctcggt ggtcagcacc gcgctcggca accgcgagat
     2161 cgtcgcctgc ctgcgccagt acgccggcga cgatccggcc gggcgcatgc tggtgcagga
     2221 agtcgtgctc gcggtcgaga cgctgctgaa gaccgacccg gcggtgttcc gcgacacgat
     2281 gacgatccgc gcctggccgc tggtgctgct cgtcgtcggc gattacgcct gggagcaccg
     2341 gctgtcgcag cccgaggcct tccggcgcgt gcagacgctc gggccgtggg cgttcatggg
     2401 acgcgtgcag gccgtggtgc gtggcgacaa tccggtcagc cggctggcgc ggctcgaatc
     2461 gctgcgccgc gacgatgccg cgcacggcct ggccgtgccc gacccgcagg aggacgccgg
     2521 agccgaggcc gtcgtcgact ggctggcctg gcgccggcgc cacggcgtgc tgacgcagct
     2581 gtcggcgcac ttctacgagc aggtctgggc cgtgctcgcg cactgcgagg ggctggtgct
     2641 cggcgagcgc ctcgacatcg gcaactgcct cgacagcgcg cggctgcgcg ccgacatgac
     2701 cgcggccgag accaacttcg cgctgaccgt caaccgcctg ctcgacaaga tccagtcgcc
     2761 cgagtaccgc cagctcaaca tcgaggcgct cgcggcgctc gcgcacctct gcagcgccaa
     2821 ccccggcctg cacgtcgccg gccacatcgt catcgacgtg ctgaccggtc acgccgtgcg
     2881 cctgcactgg ctgcagcagc atcccgatca cgccggccac tacgaagcct tccgcgccga
     2941 ggcctggagc gcgctgtacg cgagtccgcc ggccgaggtc gaatcggcgc tgatcggcgc
     3001 cttcgcccac ctgctcggcg aagcgcgtcc ggcagcggcc tgacgccgcc cgccgggagc
     3061 cgtccgcgat gaacatcggt gccgccgccc gcgcctcggg cgtctccgaa cgcatgatcc
     3121 gccactacga gaagctcggc ctgctcgccc cggccgcgcg cacggcggcg ggctaccgga
     3181 cctacacggc cggggatgtg catgtgctgc gtttcgtgcg ccgcgcgcgc gatctcggct
     3241 tcccggtggc ggacatcgcc cgactgctcg cgctgtggca ggaccgctcg cgcgccagcg
     3301 ccgaggtcaa gcgcctcgct gccggccaca tcgccgaact cgacgcgcgc atcgcgtcgc
     3361 tgagcgcgat gcgcgacgcg ctggcgcgcc tcgccggcgc ctgatgcggc gacgcgcgcc
     3421 cggac
//
LOCUS       DAOQJH010000265         3424 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_17735_length_3424_cov_4.306026, whole genome shotgun sequence.
ACCESSION   DAOQJH010000265 DAOQJH010000000
VERSION     DAOQJH010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3424)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3424)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3424
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17735_length_3424_cov_4.306026"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            71..1414
                     /locus_tag="PLQ18_07030"
     CDS             71..1414
                     /locus_tag="PLQ18_07030"
                     /inference="COORDINATES: protein motif:HMM:NF024905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HNE01445.1"
                     /translation="MTSSPAASLSSPQALAAATACFKPELYALAYERLRLDALAPTPD
                     NQAARAALAATVAKIERCVVRIQELPAIASRPVPPAEIFGGADFAAFRSPPLAGLKLL
                     IAQIRAPRSPLLRHALRLSAAVGAALALGRVLPYEAHGYWIVLTIALVLRPNFSITQQ
                     RQKDRLLGNLTGCVLAAALLAAEPPAVLLLAAIFVANTVAHAFVTLRYRYTATAGCVI
                     VLLQVHLTGSGAGEVTMLARVLDTVLGGLLSIGASHLLPSWERYAMPALLRGLLDAAG
                     AHARATLAEPMDELAYRLARKRYHDAVVALAGAFERMLAEPPDQQRAVAALERYVALG
                     YLLGTQLVALHMLRLHRGSEFEADAPERLRASAAAVAASLAAAAHAIDPAAAVPEALP
                     PPASALADTLADDALPGDWHAERLLVRRLALIRASAGELRERAGALAAAGWSAGP"
     gene            1547..1762
                     /locus_tag="PLQ18_07035"
                     /pseudo
     CDS             1547..1762
                     /locus_tag="PLQ18_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009026075.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
     gene            complement(1844..2155)
                     /locus_tag="PLQ18_07040"
     CDS             complement(1844..2155)
                     /locus_tag="PLQ18_07040"
                     /inference="COORDINATES: protein motif:HMM:NF020918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HNE01446.1"
                     /translation="MTKFSVPSLEAVTAPIVAAGKLSVAQLEKLTSFQFALVQDYAEL
                     SLSRLKAATEISDLAGLKAYGEASVGVAKDVGAKVQADAKALVELLTGFGAEYKGLVR
                     V"
     gene            2512..3054
                     /locus_tag="PLQ18_07045"
     CDS             2512..3054
                     /locus_tag="PLQ18_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013234696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron transporter"
                     /protein_id="HNE01447.1"
                     /translation="MKAPLRPVLAAVALLALPALAVAKEYPVGKPVVKNGMEIAAVYL
                     QPVRMEPDGMMRKAEDSDVHLEADIKAAKNNPNGFAEGDWMPYLSVQYELSKQGGETQ
                     SGEFMPMVASDGPHYGDNVKLQGPGKYTLTLTISPPGANPHAHFGRHTDKETGVGPWF
                     EPFKVSYDFAFAGIGKKGGY"
     gene            3084..>3424
                     /locus_tag="PLQ18_07050"
     CDS             3084..>3424
                     /locus_tag="PLQ18_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HNE01448.1"
                     /translation="MVRPSFLLRLHPGLRAVLGAALLAAAGAASAADAHTVTLIAKDG
                     RFEPATLEAPAGTRIRIEIENAGDTPIEFESTPLRKEKVLAPGARSVVVIQPLDPGRY
                     PFFDEFHPDTGR"
BASE COUNT          486 a         1270 c         1155 g          513 t
ORIGIN      
        1 ttcaatgtgt tgctgcagga gttcgaccgc ctcgccatga cgcccttcgt gctggaagag
       61 gattgccagc gtgacctcga gccccgcggc cagcttgtca tccccacagg ctctcgcggc
      121 ggcgaccgcc tgcttcaagc ccgagctcta cgcgctcgcc tacgagcgcc tgcgcctcga
      181 tgcgctggcg ccgacgccgg acaaccaggc ggcgcgcgcg gcgctggcgg cgaccgtcgc
      241 caagatcgag cgctgcgtcg tgcgcatcca ggagttgccg gccatcgcca gccggccggt
      301 gccgccggcg gagatcttcg gcggcgccga cttcgcggcc ttccgcagcc cgccgctcgc
      361 cgggctgaag ctgctgatcg cgcagatccg cgcgccgcgc tcgccgctgc tgcgccacgc
      421 cctgcgcctg agcgcggcgg tcggggcggc gctggcgctc gggcgggtgc tgccctatga
      481 ggcgcacggc tactggatcg tgctgaccat cgcgctggtg ctgcgcccga acttcagcat
      541 cacgcagcag cgccagaagg accgcctgct cggcaacctg accggctgcg tgctggccgc
      601 ggcgctgctg gcagccgagc cgccggccgt gctgctgctc gcggcgatct tcgtcgccaa
      661 caccgtcgcg cacgccttcg tgacgctgcg ctaccgctac acggccaccg ccggctgcgt
      721 gatcgtgctg ctgcaggtgc acctgaccgg cagcggggcc ggcgaggtga cgatgctcgc
      781 gcgcgtgctc gacaccgtgc tcggcggact gctgtcgatc ggtgcaagcc acctgctgcc
      841 gagctgggag cgctacgcga tgccggcgct gctgcgcggc ctgctcgatg ccgccggcgc
      901 acacgcacgc gccacgctcg ccgagccgat ggacgaactt gcctaccgcc ttgcgcgcaa
      961 gcgctaccac gacgccgtcg tcgcgctcgc cggggccttc gagcgcatgc tcgccgagcc
     1021 gcccgaccag cagcgcgcgg tcgcggcgct ggagcgctac gtcgcgctcg gctacctgct
     1081 cggcacgcag ctcgtggcgc tgcacatgct gcgcctgcac cgtggcagcg agttcgaggc
     1141 cgacgcgccc gagcgcctgc gcgccagtgc ggccgcggtc gccgccagcc tcgctgccgc
     1201 cgcgcacgcg atcgacccgg ccgccgccgt ccccgaagcc ctgccgcccc cggcgagcgc
     1261 gctcgccgac acgctcgccg atgacgcgct gcccggcgac tggcacgccg aacgcctgct
     1321 ggtgcgccgg ctggccctga tccgcgccag cgccggggag ctgcgcgagc gtgcgggtgc
     1381 gctggcggcc gcgggctgga gcgccggccc ctgagcccgg cggccgatgt ttccggcgtg
     1441 ctcccgccgg cgccgtccgc gcgccgtgcc ttcgcaccgc tccggcgctg ccctcgccga
     1501 ggtcactgct cctgccacgg ccggcgccgg atttcccgta gcgtgctcgc tcggcaagta
     1561 cagccccgtc ggcaacctga tgggctcgct ggtcggcggc aacctgtggc tggagggcgg
     1621 gttcgcgcga atgatgtatc tgtcgctgca caagctgcac aagctgcaca agctggcgct
     1681 gcacggcttc tggaaggtcg cgctcgatac gctcgcccgc atgatcacgc gccgcaccga
     1741 accgcacgtg aagctgcact gagcaccgcg caccgctgcc tgaaacgaca ccgcccggca
     1801 gaagccgggc ggtgcggggg ggcgtacagc ggacagcgtg cgatcagacg cgcacgaggc
     1861 ccttgtactc ggcgccgaag ccggtcagca gctcgaccag cgccttcgca tcggcctgga
     1921 ccttcgcgcc gacatccttc gcgacgccga ccgaggcttc gccgtaggcc ttcaggccgg
     1981 ccagatcgct gatctcggtg gcggccttca gacgcgacag gctgagctcg gcgtagtcct
     2041 gcaccagagc gaactggaac gaggtcagct tttcgagctg ggcgaccgac agcttgccgg
     2101 cggcaacgat cggggcggtg acggcttcga gggacgggac ggagaacttg gtcatggcgg
     2161 cttcctcaag gtgcaggcgg ggtgttgtgg gattcgtctg ccgaatccat gagcccatta
     2221 taggcatttc atgctgcagt gcaacatgaa tttccgctca ccctgtttgc cttgattcca
     2281 tcggactgca gccgggtccg ggcatgactg cagctgttct ccccggacgc tgcagctgcg
     2341 cgcaaccggc gggtctgtgc gcggtcgtgc ccccggtgat gcctggcgat gcagctcagg
     2401 ctgcgaggcc aaatcgttga cgttcgtcaa tggcaatgat tgtcatttgg caatgcagaa
     2461 ctatacagtg cggccgtacc cgccctttcc cgatccccag gagttcaccc gatgaaggct
     2521 ccgttgcgcc ccgtgctggc cgctgtcgcc ctgctcgccc tgcccgcgct tgccgtcgcc
     2581 aaggaatatc cggtcggcaa gccggtcgtg aaaaacggca tggagatcgc cgccgtctac
     2641 ctgcagccgg tgcgcatgga gccggacggc atgatgcgca aggccgagga ctccgatgtc
     2701 cacctcgaag ccgacatcaa ggccgcgaag aacaacccga acggtttcgc cgaaggcgac
     2761 tggatgccct acctgtcggt gcagtacgag ctgagcaagc agggcggcga gacgcagtcc
     2821 ggcgagttca tgccgatggt cgcctccgac ggcccgcact acggcgacaa cgtcaagctg
     2881 cagggcccgg gcaagtacac gctgacgctg accatcagcc cgccgggcgc caacccgcac
     2941 gcgcacttcg gccgccacac cgacaaggaa accggcgtcg gcccgtggtt cgagcccttc
     3001 aaggtcagct acgacttcgc cttcgccggc atcggcaaga agggcggcta ctgagccgcc
     3061 cgcgcccgca ggagcgccgt cccatggtcc gtccgtcctt cctcctccgt ctgcatcccg
     3121 gcctgcgcgc cgtcctcggc gccgcgctgc tcgcggcggc cggagcggcg tccgcggccg
     3181 atgcccacac cgtcacgctg atcgcgaagg acggccgctt cgagcccgcg acgctcgaag
     3241 cgccggccgg cacgcgcatc cgcatcgaga tcgagaacgc cggcgacacg ccgatcgagt
     3301 tcgagagcac gccgctgcgc aaggagaagg tgctcgcacc cggcgcgcgc tcggtggtcg
     3361 tgatccagcc gctcgacccg ggccgctacc cgttcttcga cgagttccac cccgacaccg
     3421 gccg
//
LOCUS       DAOQJH010000266         3423 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_17742_length_3423_cov_3.980107, whole genome shotgun sequence.
ACCESSION   DAOQJH010000266 DAOQJH010000000
VERSION     DAOQJH010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3423)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3423)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3423
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17742_length_3423_cov_3.980107"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..211)
                     /locus_tag="PLQ18_07055"
     CDS             complement(<1..211)
                     /locus_tag="PLQ18_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HNE01449.1"
                     /translation="MPRSPRPSNQLRVIGGRFRGRRLAFPDAPGLRPTPDRVRETLFN
                     WLMPVIDGARCLDLFAGSGALGIEAL"
     gene            325..1248
                     /gene="ftsY"
                     /locus_tag="PLQ18_07060"
     CDS             325..1248
                     /gene="ftsY"
                     /locus_tag="PLQ18_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002808872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HNE01450.1"
                     /translation="MSEASWFGRLKSRLARTRQTLSDGLGSLLLGRKQIDDELFEELE
                     TRLLMADVGIEATQHIIGRLTERVSRRQLADGAALMQALREELTALLAPCERPLDVST
                     QRPFVILMAGINGAGKTTTIGKLAQRFRSEGRSVLLAAGDTFRAAAVEQLQVWGERNG
                     VPVIAQGTGADSASVIFDAVQSAQARGTQVLIADTAGRLHTQQNLMDELRKIRRVIGK
                     IDAAAPHEVLLVLDAGTGQNALNQAVQFHEAIGVTGLVLTKLDGTAKGGIVFAIARRL
                     GLPIRYIGVGEGLEDLREFVAADYVSALLDA"
     gene            1401..2267
                     /gene="rpoH"
                     /locus_tag="PLQ18_07065"
     CDS             1401..2267
                     /gene="rpoH"
                     /locus_tag="PLQ18_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018232814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HNE01451.1"
                     /translation="MTTTALVPYAQQLPAATGDLDAYIRAVSGVPMLDADEEQDLARK
                     LQQRNDLEAARKLVLAHLRFVVHIARGYHGYGLPLGDLIQEGNVGLMKAVKRFDPEHG
                     VRLVSFAVHWIRAEIHEYILRNWRIVKVATTKAQRKLFFNLRSAKQRLGWFTHEEVET
                     VARELGVTPGDVLEMESRLSGNDLTYDPTPDDDDADGPVAPAAYLSNPADDPLAQLEA
                     EDWEARTGHRLGAALEGLDARARDILRRRWLDEDHKPTLHELAAEYGVSAERIRQIEA
                     GALKKLKTALAA"
     gene            2464..>3423
                     /locus_tag="PLQ18_07070"
     CDS             2464..>3423
                     /locus_tag="PLQ18_07070"
                     /inference="COORDINATES: protein motif:HMM:NF012730.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel"
                     /protein_id="HNE01452.1"
                     /translation="MTAAPAHHLVRCCYALAASPGYRRTKRFAYDLLENPASRLRPYF
                     DMAMCALILATVGLIIHEVKNPPTPFTLVFEDTVMYVFIAEYLARMWVWNDSHRLVIE
                     AWERAELLGLPFRLRPVLAALLRQKWAYATQPMAIIDLLAILPSYRPLRTLRFFLLFR
                     VFKLFRYTRSINEFARVLAEKRFEFYTLATFIGFVVFASATAIYILEAHVETSHVNTL
                     YDAVYWAVITIFTVGYGDIVPKTPEGRLVAIVLVVIGVAVISFSTSIVVNAFSEKMQT
                     MRDRRLYADTEKLHDYVIVCGYGRVGEVVVDRLVATGTPFVIID"
BASE COUNT          531 a         1255 c         1143 g          494 t
ORIGIN      
        1 acagcgcctc gatgccgagc gcaccgctgc cggcgaacag gtcgaggcag cgcgcgccgt
       61 cgatgaccgg catcagccag ttgaacagag tctcgcgcac gcgatccggc gtcgggcgca
      121 ggcccggagc gtcggggaac gcgagccgcc ggccgcggaa gcggccgccg atcacgcgca
      181 actggttgct cggccgggga ctgcggggca tcgggagggc tcgggcgatc ggcaacggca
      241 ggcggccggg ggcggcgatg atacgatgcg cgcgccccgc ccgccgcgcg cgggccatgt
      301 ccctgcacga gaatcccccg cgcaatgagc gaagcctcct ggttcggccg cctgaagagc
      361 cgactcgccc gcacccgcca gaccctcagc gacggcctcg gcagcctgct gctcgggcgc
      421 aagcagatcg acgacgagct gttcgaggaa ctcgaaaccc ggctgctgat ggccgatgtc
      481 ggcatcgagg ccacgcagca catcatcggg cggctgaccg agcgcgtgtc gcgccgccag
      541 ctcgccgacg gcgcggcgct gatgcaggcg ctgcgcgagg agctgaccgc gctgctcgcg
      601 ccctgcgagc gcccgctcga cgtgagcacg cagcggccgt tcgtgatcct gatggccggc
      661 atcaacggcg ccggcaagac cacgaccatc ggcaagctcg cgcagcgctt ccgcagcgag
      721 ggcaggagcg tgctgctcgc cgccggcgac accttccgcg ccgccgccgt cgagcagctg
      781 caggtctggg gcgagcgcaa cggcgtgccg gtgatcgcgc agggcaccgg tgccgattcg
      841 gcctcggtga tcttcgacgc cgtgcagtcg gcgcaggcgc gcggcacgca ggtgctgatc
      901 gccgacaccg ccgggcgcct gcacacgcag cagaacctga tggacgagct gcgcaagatc
      961 cgccgcgtca tcggcaagat cgacgccgcc gcgccgcacg aggtgctgct ggtgctcgac
     1021 gccggcaccg gccagaacgc gctgaaccag gccgtgcagt tccacgaggc gatcggcgtc
     1081 accggtctgg tgctgaccaa gctcgacggc accgccaagg gcggcatcgt gtttgcgatc
     1141 gcgcgccgcc tcggcctgcc gatccgctac atcggcgtcg gcgaggggct ggaggacctg
     1201 cgcgagttcg tcgccgccga ttacgtctcg gcgctgctcg acgcctgaag cttcgcggaa
     1261 agcctcgcga aaccactgcc gatacaggcg cgtatcgccg ctcaaccggg ggcgccggcg
     1321 gaactcccgc cggctttggc actcctatcg cccgactgct aacatcagcc cgcgaactgg
     1381 agttctctgg aggacttcgc atgaccacga ccgcactggt tccctacgcg caacagcttc
     1441 cggccgccac cggcgacctc gacgcctaca tccgcgcggt ttccggggtg ccgatgctcg
     1501 acgcggacga ggaacaggac cttgcccgca agctgcagca acgcaacgac ctcgaagcgg
     1561 cgcgcaagct cgtgctcgcg cacctgcgct tcgtcgtgca catcgcccgc ggctaccacg
     1621 gctacgggct gccgctcggc gacctgatcc aggaaggcaa cgtcggcctg atgaaggccg
     1681 tcaagcgctt cgacccggag cacggcgtgc gcctcgtcag cttcgccgtg cactggatcc
     1741 gcgccgagat ccacgaatac atcctgcgca actggcgcat cgtcaaggtc gcgaccacga
     1801 aggcgcagcg caagctgttc ttcaacctgc gcagcgccaa gcagcgcctc ggctggttca
     1861 cgcacgagga agtcgagacc gtcgcgcgcg aactcggcgt gacccccggc gacgtgctgg
     1921 agatggaaag ccggctgtcc ggcaacgacc tgacctacga cccgacgccg gacgacgacg
     1981 acgccgacgg cccggtggcg ccggccgcgt acctgtcgaa tccggccgac gatccgctcg
     2041 cgcaactcga agccgaggac tgggaggcgc gcaccggcca ccggctcggg gccgcgctcg
     2101 aaggcctcga tgcccgcgcc cgcgacatcc tgcgccggcg ctggctcgac gaggaccaca
     2161 agccgacgct gcacgagctg gccgccgagt acggcgtctc ggccgagcgc atccgccaga
     2221 tcgaggccgg cgcgctgaag aagctgaaga ccgcgctcgc cgcctgaacc tcacgccctt
     2281 ccctgccccg acgccggggc cgtccgctgc gacggccccg gcgtcggctt tcatgcgggc
     2341 ggccgggggc cacccgatcg tcccgcttga ccgggatcgc tgcaggcacg gccggctgcg
     2401 gccatactgc cccacctctg tctccttccg tcggcacggc cgcccgcccg aaccttcgcg
     2461 accatgaccg ccgcgcccgc ccatcacctc gtccgctgct gctatgcgct cgccgccagt
     2521 cccggctacc ggcgcaccaa gcgcttcgcc tacgacctgc tcgaaaaccc ggcctcgcgg
     2581 ctgcggccgt acttcgacat ggcgatgtgc gcgctgatcc tcgcgacggt cgggctgatc
     2641 atccacgagg tcaagaaccc gccgacgccg ttcacgctcg tgttcgagga cacggtcatg
     2701 tacgtgttca tcgccgagta cctggcgcgc atgtgggtgt ggaacgactc gcaccggctg
     2761 gtgatcgagg cctgggagcg cgccgagctg ctcggcctgc cgttccggct gcggccggtg
     2821 ctcgctgcgc tgctgcgcca gaagtgggcc tatgcgacgc agccgatggc aatcatcgac
     2881 ctgctggcga tcctgccctc gtaccggccg ctgcgcacgc tgcgcttctt cctgctgttc
     2941 cgcgtcttca agctgttccg ctacacgcgc tcgatcaacg agttcgcgcg cgtgctcgcc
     3001 gagaagcgct tcgagttcta cacgctggcg accttcatcg gcttcgtcgt gttcgcgtcg
     3061 gcgaccgcga tctacatcct cgaagcccac gtcgagacca gccacgtcaa cacgctctac
     3121 gacgccgtct actgggcggt gatcacgatc ttcacggtcg gctacggcga catcgtgccg
     3181 aagacgcccg agggccggct ggtggcgatc gtgctggtgg tgatcggcgt ggcggtgatc
     3241 tcgttctcga cctcgatcgt cgtcaacgcc ttcagcgaga agatgcagac gatgcgcgac
     3301 cggcgcctgt acgccgatac cgagaagctg cacgactacg tgatcgtctg cggctacggc
     3361 cgggtcggcg aggtcgtcgt cgaccggctg gtggccaccg gcacgccgtt cgtgatcatc
     3421 gac
//
LOCUS       DAOQJH010000267         3422 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_17753_length_3422_cov_3.568756, whole genome shotgun sequence.
ACCESSION   DAOQJH010000267 DAOQJH010000000
VERSION     DAOQJH010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3422)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3422)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3422
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17753_length_3422_cov_3.568756"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..817
                     /locus_tag="PLQ18_07075"
     CDS             <1..817
                     /locus_tag="PLQ18_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNE01453.1"
                     /translation="ALHGVAAWLATLGCALPLVIGFVLPAGRLLALAHADGDAQLGPR
                     FLTLAGNSLAISALTAALATLLALALAYGARLARGPARRAAGLLNRGIALGYAVPGSV
                     IAVGILIPVTRLDHAIAALWQSLGQPHPGLLLTGGLAALVYGYLVRFLSVAVQTVDTG
                     LSRITVSMDEAARSLGRGRGALLREVHLPLLRGSLASAALLVFVDTMKELPATLVMRP
                     FNFDTLATQVYTLAADERLAEASTAALAIVAVGVLPLILLSRHIAQGTQPQDS"
     gene            908..1498
                     /locus_tag="PLQ18_07080"
     CDS             908..1498
                     /locus_tag="PLQ18_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HNE01454.1"
                     /translation="MARRILILDGHPDRDPAPHFLPALLDAYADGARAAGHEVERLRV
                     ATLDFPWLRSGGDWEPANTPPDVTRLQAALTAADHWLLAYPLWFGNPPALLLGLLEHA
                     LRPGFAFTPPGPGAPPKPLLGGRSAHVLVTMNMPAFAYRWYFGAHGVKTLRRHLLGTA
                     GFRPVRTTLVGSVLTIGTGGRERWLQRLRAMGTEGR"
     gene            1547..2005
                     /locus_tag="PLQ18_07085"
     CDS             1547..2005
                     /locus_tag="PLQ18_07085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE01455.1"
                     /translation="MLSAPTAEATTAAAMRPASTALLLLAALAALPGCSTARLPPPED
                     LAGVPGYYASGRRGAVYYDVAIYLDADNRGAVVMDGGCEGPPRILPQTVVRSEGYVSF
                     QAMGCRDPRYAGVEVTQAELADGKVLGAELYLLRRRDSVLERGPSMTLSP"
     assembly_gap    2128..2227
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2324..3157)
                     /locus_tag="PLQ18_07090"
     CDS             complement(2324..3157)
                     /locus_tag="PLQ18_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006747566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNE01456.1"
                     /translation="MNPASVLAPVLTVAAKEFRDGLRNRWVAAIAIVCALLAVGLATF
                     GAAASGQVGFTGLATTIASLASLAVFLIPLIALLLAYDSIVGEDEQGTLLLLLTYPLS
                     RLQLLAGKFLGHGAILAVSTTLGFGLAGLVIGIGSDKVATGPLVAALGLFIVSATLLG
                     WAFVGMAYVISAGASEKSRAAGLALVVWFAFVLVFDLGLLGFLVATGGAIGRDWLPWL
                     LLLNPADVFRLANLAGFDANATAGPVSIGAGFPVPLLLAVQGAWVLAPFALAVWRFRR
                     R"
     gene            complement(3154..>3422)
                     /locus_tag="PLQ18_07095"
     CDS             complement(3154..>3422)
                     /locus_tag="PLQ18_07095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE01457.1"
                     /translation="KLDALRGLLIHVRGNPGPHALAALPAGSRTRMLADGRFEIALAV
                     HAKLDALRGLLTDPAVHDLDIEPPSLETLYAHFGSAVAGEGESA"
BASE COUNT          458 a         1241 c         1190 g          433 t
ORIGIN      
        1 ggcgctgcac ggcgtggccg cgtggctcgc aacgctcggc tgcgcgctgc cgcttgtgat
       61 cggcttcgtg ctgccggccg ggcggctgct cgcgctcgcg cacgccgatg gcgatgcgca
      121 gctcggtccg cgcttcctga cgctggccgg caacagcctg gcgatctcgg cgctgaccgc
      181 cgcgctcgcc acgctgctcg cgctcgcgct cgcctacggc gcccggctcg cgcgcggccc
      241 ggcgcgccgg gccgccgggc tgctgaaccg cggcatcgcc ctcggttacg cggtgccggg
      301 ctcggtgatc gcggtcggca tcctgatccc ggtgacacgg ctcgatcatg ccatcgccgc
      361 gctctggcag tcgctcggcc agccgcatcc gggcctgctg ctgaccggcg ggctcgcggc
      421 gctggtctac ggctatctgg tgcgtttcct gagcgtcgcg gtgcagacgg tcgacacggg
      481 cctgtcgcgc atcaccgtca gcatggacga ggccgcgcgc agcctcgggc gcgggcgcgg
      541 cgcgctgctg cgcgaggtgc acctgccgct gctgcgcggc agcctcgcga gcgccgcgct
      601 gctggtgttc gtcgacacga tgaaggaact gccggcgacg ctggtgatgc gcccgttcaa
      661 cttcgacacg ctggcgaccc aggtctacac gctggcagcg gacgaacgcc tcgccgaggc
      721 ctcgaccgcg gcgcttgcga tcgtcgcggt cggcgtgctg ccgctgatcc tgctgtcacg
      781 gcacatcgcg cagggaacgc agccgcagga cagctgaatc ctgccccggc gccggcaacg
      841 gggatggcca gcagccgggg gacggatgca tgctgcaacg catcacctgc ttgcggagtt
      901 caccggcatg gcccgacgca tcctgatcct cgacggtcac ccggaccgcg atccggcacc
      961 gcacttcctg ccggccctgc tcgatgccta tgccgatggc gcgcgcgcgg ccgggcacga
     1021 agtcgagcgg ctgcgcgtcg ccaccctcga cttcccgtgg ctgcgcagcg gcggcgactg
     1081 ggagccggcg aacacgccgc ccgatgtcac gcgcctgcag gcggcgctca cggccgccga
     1141 tcactggctg ctcgcgtatc cgctgtggtt cggcaacccg ccggcgctgc tgctcggcct
     1201 gctcgaacac gcgctgcgcc ccggcttcgc gttcacgccg cccggccccg gcgcgccgcc
     1261 gaaaccgctg ctcggcgggc gcagcgcgca cgtgctggtg acgatgaaca tgccggcctt
     1321 cgcctaccgc tggtacttcg gtgcccacgg cgtgaagacg ctgcgccggc acctgctcgg
     1381 caccgcgggc ttccgcccgg tgcgcacgac gctggtcggc agcgtgctga cgatcggcac
     1441 cgggggccgc gaacgctggc tgcagcggct gcgggcgatg ggcacggagg gccggtgacg
     1501 ccgcccgatt gccctgggcc cctaccggcg cgccggcgag gccggcatgc tgtcggcccc
     1561 gaccgccgag gccacgaccg ctgccgccat gcgccctgca tcgaccgccc tgctgcttct
     1621 cgccgccctc gccgccctgc ccggctgctc gaccgcgcgg ctgccaccgc ccgaggacct
     1681 cgccggcgtg cccggctatt acgcgagcgg ccggcgcggc gccgtgtact acgacgtcgc
     1741 gatctacctc gacgccgaca accgcggtgc cgtggtcatg gacggcggct gcgagggacc
     1801 gccgcgcatc ctgccgcaga cggtcgtgcg ctccgagggt tacgtcagct tccaggccat
     1861 gggctgccgt gacccgcgct atgccggcgt cgaggtcacg caggccgaac tcgccgacgg
     1921 caaggttctc ggggccgagc tgtacctgct gcgccggcgc gacagcgtgc tcgaacgcgg
     1981 gccgtcgatg acgctgtcgc cgtaaggccg cacaaccttc cgtgccggct ccggccccgc
     2041 tcctgcggcc ggatcacccg cctgcccggt gccccgctca gcccggctcc agcccgccgg
     2101 ccaccagcgc cacggtcacg tcgaggannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnggc gccgagaccg agcaggacgt ggcggcggct gggcatgtgc ggaacctctc
     2281 gcggtgaccg gaacggacgg gctgcaacga caacggcgat gactcagcgt cgccggaagc
     2341 gccagacggc gagcgcgaac ggcgcgagca cccacgcgcc ctgcacggcc agcagcagcg
     2401 gcacggggaa gccggcaccg atcgacaccg ggccggccgt ggcgttcgcg tcgaagccgg
     2461 ccaggttcgc aagccggaac acgtcggccg ggttcagcag cagcagccag ggcagccagt
     2521 cgcgcccgat cgcgccgccg gtcgcgacca gaaagccgag caggccgaga tcgaacacca
     2581 gcacgaacgc gaaccagacg accagcgcga gcccggcggc gcgggatttc tcgctggcac
     2641 cggcgctgat cacataggcc ataccgacga aagcccagcc gagcagcgtc gcgctgacga
     2701 tgaacagccc gagggccgcc accagcggcc cggtcgcgac cttgtccgag ccgatgccga
     2761 tgactaggcc ggcgagcccg aaaccgagcg tcgtcgatac ggcgaggatc gcgccgtgac
     2821 cgaggaactt gccggcgagc agctgcagcc gcgacagcgg ataggtcagc agcagcagca
     2881 gcgtgccctg ttcgtcctcg ccgacgatgc tgtcgtaggc gagcagcagc gcgatcagcg
     2941 gaatcaggaa taccgcgagg ctcgctagac tcgcgatcgt ggtcgccagc cccgtgaagc
     3001 cgacctgccc cgaggccgca gccccgaagg tcgccagccc cacggcgagc agcgcgcaga
     3061 cgatcgcgat cgccgccacc cagcggttgc gcaggccgtc gcggaactcc ttggcggcga
     3121 cggtcagcac cggcgcgagc accgatgcgg gattcatgcg gattctcctt caccggcgac
     3181 ggcgctgccg aagtgggcgt acagggtttc gagcgacggc ggctcgatgt cgagatcgtg
     3241 cacggccgga tcggtcagca ggccgcgcag tgcgtcgagc ttggcgtgca cagccagcgc
     3301 gatctcgaag cggccatcgg cgagcatgcg cgtgcggctg ccggccggca gtgcggcgag
     3361 cgcgtgcggt ccggggttgc cgcgcacgtg gatcagcagg ccgcgcagtg cgtcgagctt
     3421 gg
//
LOCUS       DAOQJH010000268         3416 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_17806_length_3416_cov_4.451056, whole genome shotgun sequence.
ACCESSION   DAOQJH010000268 DAOQJH010000000
VERSION     DAOQJH010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3416)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3416)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3416
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17806_length_3416_cov_4.451056"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1017
                     /locus_tag="PLQ18_07100"
     CDS             <1..1017
                     /locus_tag="PLQ18_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011384931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNE01458.1"
                     /translation="PGDHALHAFAEGWQLPVAAGFRRQDIFDNRRAQYAGELGTSVAP
                     TLARRVREADLLLVLGSRLGEMTTNGYTLVEAPLPRQTLIHVHPDALELNRVYRPALA
                     LATHPAQAAVQLAARPAPAALRWAEWTAAARAEHEANRQPDPMAGALDLGEVMRVLDA
                     ALPADAIVCNGAGNYTGWPQRFHCFSHYPSQLAPTSGAMGYGVPAAIAAAAVHPTRIA
                     VAFAGDGCFQMNGQELASAVQAGLKPLILVIDNGQYGTIRMHQERHFPDRRIATALCN
                     PDFAALARACGAFGERIECTGAFAPALERALDCDRAALLHLIVDPEQISTRQTLTALR
                     AATR"
     gene            1051..2550
                     /locus_tag="PLQ18_07105"
     CDS             1051..2550
                     /locus_tag="PLQ18_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013395347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNE01459.1"
                     /translation="MSDPLLRSLGLSPDALRDGDLIARSPIDGTVLARVRSHTPADID
                     AAIARAHLAFHRWRTVPAPRRGELVRLLGEELRANKAALGALVTLETGKIAAEGEGEV
                     QEMIDICDFAVGLSRQLHGLTIASERPGHRMMETWHPLGVTGVISAFNFPVAVWAWNF
                     ALALVCGNPVVWKPSEKTPLSALACQALFRRALLRWGEAPEGLSELLIGARAAGEQLA
                     DDARVALVSATGSTAMGRVLGPRVAARFGRTLLELGGNNAVIVAPSADLELALRGIVF
                     GAIGTAGQRCTSTRRLIVHTSIHDALLERLQAAFVSARVGDPRQAGTLVGPLIDEAAF
                     TAMQSALAAAVAEGGRLVCGGERVLKVRMPEAFYVTPALVTMPAQSAAVRRETFAPIL
                     YVLRYRTLDEAIALNNDVPQGLSSAIFTNDLREAGQFLGATGSDCGIANVNLGTSGAE
                     IGGAFGGDKETGGGRESGSDAWKSYMRRATNTLNESRELPLAQGIRFGS"
     gene            complement(2650..3207)
                     /locus_tag="PLQ18_07110"
     CDS             complement(2650..3207)
                     /locus_tag="PLQ18_07110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE01460.1"
                     /translation="MISMKQPSLVLHSHNVPGYKYQMWATWTVPKSLPASSLVDWIVS
                     AVDRSPEMALKNIIINTHGSPGYLHIGSGVGVKDLNEFKRLRGKSIGYIWVIACEVSK
                     GVDLGNVCPVDEYNGPYFCTELAKASGTFVVASEELQSVDPGFTVFSVFVPSGYVDDY
                     EGAVYRYSPAGGRTRISANGSDAYD"
BASE COUNT          493 a         1268 c         1168 g          487 t
ORIGIN      
        1 ccaggagatc acgcgctgca tgccttcgcc gaaggctggc agctgccggt cgccgccggc
       61 ttccgccggc aggacatctt tgacaaccgc cgcgcgcagt acgccggcga gctcggcacc
      121 tcggtcgcgc cgacgctggc gcggcgcgtg cgcgaggccg acctgctgct ggtgctcggc
      181 tcgcgcctcg gcgagatgac gacgaacggc tacacgctgg tcgaagcgcc gctgccgcgc
      241 cagacgctga tccacgtcca ccccgatgcg ctggagctga accgcgtcta ccgcccggcg
      301 ctcgcgctcg cgacgcaccc ggcgcaggcc gccgtgcagc tcgccgcgcg cccggccccg
      361 gccgccctcc gctgggcgga gtggaccgcc gcggcgcgcg ccgagcacga ggccaaccgc
      421 cagcccgacc cgatggccgg ggcgctcgat ctcggcgagg tcatgcgcgt gctcgatgcc
      481 gcgctgccgg ccgatgccat cgtctgcaac ggcgccggca actacaccgg ctggccgcag
      541 cgcttccact gcttctcgca ctatccgtcg cagctcgccc cgaccagcgg cgcgatgggc
      601 tacggcgtgc cggccgcgat cgccgcggcg gccgtgcatc cgacgcgcat cgccgtcgcc
      661 ttcgccggcg acggctgctt ccagatgaac ggccaggaac tggcgagcgc ggtgcaggcc
      721 ggtttgaagc cgctcatcct cgtcatcgac aacggccagt acggcaccat ccgcatgcac
      781 caggaacgcc acttcccgga ccggcgcatc gccacggcgc tctgcaaccc cgacttcgct
      841 gccctcgccc gcgcctgcgg cgccttcggc gagcgcatcg aatgcaccgg cgccttcgcg
      901 ccggccctcg aacgcgcgct cgactgcgat cgcgcggccc tgctgcacct catcgtcgat
      961 cccgaacaga tcagcacgcg ccagacgctg acggcgctgc gcgcggccac gcgctgagcg
     1021 ttctcccctt tccgcatcga gtccttgccc atgtccgacc ccctgctccg ctccctcggc
     1081 ctcagccccg atgcgctgcg cgacggtgac ctgatcgccc gttcgccgat cgacggcacc
     1141 gtgctcgcgc gcgtgcgcag ccacacgccc gccgacatcg acgcggcgat cgcccgggcg
     1201 catctggcct ttcacagatg gcgcacggtg ccggcgccgc ggcgcggcga gctggtgcgg
     1261 ctgctcggcg aggagctgcg cgccaacaag gccgcgctcg gcgcgctggt gacgctcgaa
     1321 accggcaaga tcgccgccga gggcgagggc gaagtgcagg agatgatcga catctgcgac
     1381 ttcgccgtcg gcctgtcgcg gcagctgcac ggcctgacca tcgcctccga gcgtcccggt
     1441 caccggatga tggaaacctg gcatccgctc ggcgtcaccg gcgtcatcag cgccttcaac
     1501 ttcccggtcg cggtctgggc ctggaacttc gcgctggcgc tggtctgcgg caatccggtg
     1561 gtctggaagc cgtccgagaa gacgccgctg agcgcgcttg cctgccaggc gctgttccgg
     1621 cgcgcgctgc tgcgctgggg cgaggcgccc gagggcctct cggagctgct gatcggcgcg
     1681 cgcgcggccg gtgagcagct cgccgacgat gcgcgcgtcg cgctggtgtc ggcgaccggc
     1741 tcgacggcga tgggccgggt actcggcccg cgcgtcgcgg cacgcttcgg gcgcacgctg
     1801 ctcgaactcg gcggcaacaa cgccgtgatc gtcgcgccct cagccgatct cgaactggcg
     1861 ctgcgcggca tcgtcttcgg cgccatcggc accgccggcc agcgctgcac cagcacgcgc
     1921 cggctgatcg tgcacacgtc gatccacgat gcgctgctcg aacgcctgca ggcggccttc
     1981 gtcagcgccc gcgtcggcga tccgcgtcag gccggcacgc tggtcgggcc gctgatcgac
     2041 gaagccgcgt tcacggccat gcagagcgcc ctcgccgcgg ccgtcgccga aggcggccgg
     2101 ctggtctgcg gcggcgagcg cgtgctgaag gtgcgcatgc ccgaagcctt ctacgtgacg
     2161 ccggcgctgg tgacgatgcc ggcgcagagc gcggccgtgc gccgcgagac cttcgcgccg
     2221 atcctgtacg tgctgcgcta ccgcacgctc gacgaggcga tcgcgctcaa caacgacgtg
     2281 ccgcaggggc tgtcctcggc gatcttcacg aacgatctgc gcgaggccgg gcagttcctc
     2341 ggcgcgaccg gctcggactg cggcatcgcc aacgtcaacc tcggcacctc gggcgccgag
     2401 atcggcggtg ccttcggcgg cgacaaggaa accggcggcg gacgcgaatc cggctccgat
     2461 gcctggaagt cctacatgcg ccgcgcgacc aacaccctca atgaatcgcg cgaactgccg
     2521 ctggcgcagg gcatccgctt cggcagctga cggcaacgga cggcggcccc ggccgtcgtg
     2581 ccgccgctca tccccgcggg gtcgggatga gggctgccgc cggccggcgg agggaatcag
     2641 acaggggaat cagtcatacg catcggagcc attggccgag atgcgcgtgc gcccgccggc
     2701 cgggctgtag cgatagaccg ccccctcgta gtcatcgacg tagcccgagg gcacgaacac
     2761 cgagaacacc gtgaagccgg gatcgaccga ctgcagttcc tcggaggcga cgacgaaggt
     2821 gccgctggcc ttggcgagct cggtgcagaa gtacgggccg ttgtactcgt ccaccgggca
     2881 gacgttgccg agatcgacgc ccttgctgac ctcgcaggcg atcacccaga tgtagccgat
     2941 cgacttgccg cgcaggcgct tgaattcgtt gagatccttc acgccgacgc cgctgccgat
     3001 gtgcaggtag ccgggcgagc cgtgcgtgtt gatgatgatg ttcttcagcg ccatctccgg
     3061 cgagcggtcg acggccgaga cgatccagtc gaccagcgaa ctcgccggca ggctcttcgg
     3121 caccgtccag gtcgcccaca tctggtactt gtaaccgggc acgttgtgcg aatgcagcac
     3181 gagcgaaggc tgcttcatcg aaatcatgac gatcactccg cggtggcccg cgccgggcag
     3241 cgcggtaagc gcaatctagg tgcgccgggg ccggtctgcg gaaaatcctg tcagatcagg
     3301 gtgtcatcgc gtccgggcgc gggcccggca gcgtcgagct tccgcaatac gcgggacggc
     3361 tcctcccccc ggacaggaac agaccggcaa tagtgccgat gcgcccgcgc cgtcag
//
LOCUS       DAOQJH010000269         3415 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_17819_length_3415_cov_3.628274, whole genome shotgun sequence.
ACCESSION   DAOQJH010000269 DAOQJH010000000
VERSION     DAOQJH010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3415)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3415)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3415
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17819_length_3415_cov_3.628274"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..960
                     /locus_tag="PLQ18_07115"
     CDS             <1..960
                     /locus_tag="PLQ18_07115"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNE01461.1"
                     /translation="RAAAQAQALDAGERQRLAAEAGIAGLNLRLSRLQGDLERLQQAL
                     ALAEDQRRQDAEAHAAALATERRQAAATLAEAHTQAAATLGVEHQRAAVERETAAAQL
                     AALEAEKARLERERAALAGRLDEQATALAATQTKLAERDATVTEQAERLDELDRQVRL
                     RLVERVEQLSRYASEFFGRLRTLFEDDPDIRVVGDRFVFQSEVLFGSGEAALSEAGKP
                     DLDKFAGVYRQLSGRLPEDLPVIIEIIGHTDREPIRASRFRSNWELSTARAQAVVDYL
                     IRQGIPPQRLAAVGMGEFHPVDPADTPEAFRRNRRIELKITSR"
     gene            complement(968..1741)
                     /locus_tag="PLQ18_07120"
     CDS             complement(968..1741)
                     /locus_tag="PLQ18_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNE01462.1"
                     /translation="MNELAGFLVGTVVGFTGVGGGALMTPILLFFFGVAPQTAVGTDL
                     LFAAITKFAGSLVHGSRGSIDWLVLRRLCYGSLPAAALTLYVLHGQRIDGEVQTVILR
                     TLGIALALTSLSVLFKNQMHLIGRKLRIGRPERFKRFQGPLTIFAGTILGALVTLTSV
                     GAGALGAVMLLYLYPLRLTPAKLVGTDLAHAIPLTLVAGLGHLSLGNIDTTLLGGLLT
                     GSIPGVLLGSWLSHRLDGAWVARGIAVLLLISGGKLLAI"
     gene            complement(1886..2347)
                     /locus_tag="PLQ18_07125"
     CDS             complement(1886..2347)
                     /locus_tag="PLQ18_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HNE01463.1"
                     /translation="MWLEREDFRTAVRLSPLVSIDLVVRDAAGRVLLGWRNNRPAQHC
                     WFVPGGSIRKQETLDAAFRRISHDELGLDCERTEARWLGVYEHFYDDHFAGTDFGTHY
                     VVLGHELAVGSRLTTLPDAQHAHWRWFEVDELLASPEVHRHSKWYFDPRAC"
     gene            2492..2971
                     /gene="rraA"
                     /locus_tag="PLQ18_07130"
     CDS             2492..2971
                     /gene="rraA"
                     /locus_tag="PLQ18_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008061048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008428 - ribonuclease inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0051252 - regulation of RNA metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HNE01464.1"
                     /translation="MGFKTADLCDEHADRLQIAEPVFGDYGGEISFFGPITTLKAFED
                     NSLVRKAVEEKGEGRVLVIDSGGSMRCAMVGDQLAALAEKNGWAGVIVNGCIRDSAAI
                     ADTEIGCKALGVHPLKTVKRNEGQRDVTLRFAGVSFVPGHYLYADEDGLVVSEQPLF"
     gene            3067..>3415
                     /locus_tag="PLQ18_07135"
     CDS             3067..>3415
                     /locus_tag="PLQ18_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tex-like N-terminal domain-containing protein"
                     /protein_id="HNE01465.1"
                     /translation="MNLSQTIATELAVRPAQVEAAVRLLDEGATVPFIARYRKEVTGG
                     LDDTQLRTLEERLGYLRELEDRRAAVLASIGEQGRLTDALRADILAADTKTRLEDLYL
                     PYKPKRRTKAQIAR"
BASE COUNT          576 a         1185 c         1171 g          483 t
ORIGIN      
        1 cgcgccgccg cccaggcaca ggccctcgac gccggcgaac gccagcggct ggcggcggag
       61 gccggcatcg ccgggctgaa cctgcgcctg tcgcggctgc agggtgatct cgagcgcctg
      121 cagcaggcgc tggctctcgc cgaggatcag cgccggcagg acgccgaggc ccatgccgcg
      181 gcgctggcga ccgagcgccg gcaggcggcg gcgacgctcg cggaggcgca cacgcaggct
      241 gcggcgacgc tgggcgtcga acatcagcgc gcggcggtcg aacgcgagac cgccgccgcg
      301 cagctcgcgg ccctggaggc cgagaaagcc cggctcgaac gcgagcgcgc cgcgctggcc
      361 gggcggctgg acgaacaggc gacggcgctc gccgcgacgc agacgaagct cgccgagcgc
      421 gacgcgaccg tcaccgaaca ggccgagcgc ctcgatgaac tcgaccggca ggttcggctg
      481 cggctggtcg agcgcgtcga gcagttgtcg cgctacgcgt cggagttctt cgggcggctg
      541 cgcacgctgt tcgaggacga tcccgacatc cgcgtcgtcg gcgatcgctt cgtgttccag
      601 tccgaggtgc tgttcggctc cggcgaggcc gcgctcagcg aggccggcaa gcccgatctc
      661 gacaagttcg ccggcgtcta ccggcagttg tccgggcggc tgcccgagga tctgccggtc
      721 atcatcgaga tcatcggcca caccgaccgc gagccgatcc gcgccagccg cttccgctcg
      781 aactgggagc tgtcgacggc gcgcgcgcag gcggtcgttg actacctgat ccgtcagggc
      841 atcccgccgc agcggctggc ggcggtcggc atgggcgagt tccatccggt cgatcccgcc
      901 gacacgccgg aggccttccg gcgcaaccgg cgcatcgagc tgaagatcac cagccgctga
      961 ggccgcttca gatcgccagc agcttgccgc cgctgatcag cagcagcacg gcgatgccac
     1021 gcgcgaccca ggcaccgtcg agccggtgac tgagccagga gccgagcagc acgccgggga
     1081 tcgagccggt cagcaggccg ccgagcaggg tggtgtcgat gttgccgagc gacaggtgac
     1141 cgaggccggc gaccagcgtc agcggaatcg cgtgcgcgag atcggtgccg accagcttcg
     1201 ccggcgtcag gcgcagcgga tagaggtaca gcagcatcac ggccccgagc gcgccggcac
     1261 cgaccgaggt cagcgtcacc agcgcaccga ggatggtgcc ggcgaaaatt gtcagcggac
     1321 cctggaagcg cttgaagcgt tccggccggc cgatgcgcag cttgcgcccg atcaggtgca
     1381 tctggttctt gaacagcacc gacaggctgg tcagcgccag cgcgatgccg agcgtgcgca
     1441 ggatcaccgt ctgcacctcg ccgtcgatcc gctgaccgtg cagcacgtac agcgtcaggg
     1501 ccgcggccgg caggctgccg tagcagagcc ggcgcagcac cagccagtcg atcgagccgc
     1561 gcgagccatg gacgagcgag ccggcgaact tggtgatcgc cgcaaacagc aggtcggtgc
     1621 cgaccgcggt ctgcggggcc acgccaaaga agaagagcag gatcggcgtc atcagggcac
     1681 cgccgccgac accggtgaat cccaccaccg tgccgaccag aaaaccggcc agttcattca
     1741 tggtcaagca ttcctgaagc aattgaaatc gggatcacag gcccgtgccg ctgccggtgt
     1801 gcggcatccg ggcgatacgt ttcattatta cattatgaat aatgttgttt taatgattaa
     1861 aagttatatc catcgtgccc ggttttcagc aggcccgcgg atcgaagtac cacttcgaat
     1921 gccggtgcac ctcggggctg gcgagcagtt cgtcgacttc gaaccagcgc cagtgcgcgt
     1981 gctgggcatc gggcagcgtc gtcagccgcg agccgaccgc cagctcgtgg ccgagcacga
     2041 cgtaatgggt gccgaagtcg gtgccggcaa agtgatcgtc gtagaagtgt tcgtagacgc
     2101 cgagccagcg cgcttcggtg cgttcgcagt ccaggccgag ctcgtcatgg ctgatgcgcc
     2161 ggaacgccgc gtccagggtt tcctgcttgc ggatgctgcc gcccggcacg aaccagcagt
     2221 gctgtgccgg gcggttgttg cgccagccga gcagcacgcg gccggcggca tcgcgcacga
     2281 ccagatcgat cgacaccagc ggcgacaggc gcacggcggt cctgaaatcc tcgcgttcga
     2341 gccacatgcg tccagacttc cggggggcag gcgcggccgg tttgccgcac tgccgcatgc
     2401 tacactcgcg cgccttgccg gtgccgcagt tcgccagcgt gatttgcatc tccatcaccg
     2461 cttcagaccc gctcgcagga gactccgacc gatgggcttc aagactgccg acctctgtga
     2521 cgaacacgcc gaccgcctgc agatcgccga accggtgttc ggtgactacg gcggcgagat
     2581 cagcttcttc ggtccgatca cgacgctgaa ggccttcgag gacaactcgc tggtgcgcaa
     2641 ggccgtcgag gaaaaaggcg agggccgggt gctggtcatc gacagcggcg gctcgatgcg
     2701 ctgcgcgatg gtcggcgacc agctcgcggc gctggccgag aagaacggct gggccggcgt
     2761 catcgtcaac ggctgcatcc gcgactcggc agccattgcg gataccgaga tcggctgcaa
     2821 ggcgctcggc gtgcacccgc tgaagaccgt caagcgcaac gagggccagc gcgacgtgac
     2881 gctgcgcttc gccggcgtca gcttcgtgcc cggacactac ctctatgccg acgaggacgg
     2941 tctggtcgtc tcggaacagc cgctgttctg aggcgggccg ctgtcctttt ttcctgactc
     3001 cgccgcatcg cccccagcgc cggttccgga gccatcgccc ccggaaccgc cggtttgccg
     3061 cccgccatga acctctccca gaccatcgcc accgaactgg ccgtgcggcc cgcgcaggtg
     3121 gaggccgccg tgcgcctgct tgacgagggc gcgaccgtgc cgttcatcgc ccgctaccgc
     3181 aaggaagtca ccggcggcct cgacgacacg cagctgcgca cgctcgagga acgtctcggc
     3241 tacctgcgcg agctggaaga ccgccgcgcc gccgtgctcg ccagcatcgg cgagcagggc
     3301 aggctgaccg acgcgctgcg ggccgacatc ctcgctgccg acaccaagac ccggctcgaa
     3361 gacctgtacc tgccgtacaa gcccaagcgc cgcaccaagg cgcagatcgc ccgcg
//
LOCUS       DAOQJH010000270         3407 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_17884_length_3407_cov_3.092482, whole genome shotgun sequence.
ACCESSION   DAOQJH010000270 DAOQJH010000000
VERSION     DAOQJH010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3407)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3407)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3407
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17884_length_3407_cov_3.092482"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1119
                     /locus_tag="PLQ18_07140"
     CDS             <1..1119
                     /locus_tag="PLQ18_07140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE01466.1"
                     /translation="EGGDAAAEAGAAVEAGGGESFASTETIETTETTEAVESVETAPA
                     RSSFAVPAGRYVRNGRPRLPRGTLANADAVTGAPEPVTGLVTAAVTAVAVAGAAEAVA
                     GEAPVTTDAAETTAAEPTMEAAAGSVPVETPAPVPEAAAPVEAPAAATDAPAEAAVPA
                     ETAPVAEDAPVPEDAPVPVVDGDIAEAVAEAVADILGEAQVAAGAIADMPAEAPETPA
                     EVMGDVEAGDTVVEAAVAVTEAPVPEMAGATDTPAIEAAPDGQPEVVVPPLPVDAPQP
                     VLPEAPVTTPEVLAGTEAAATTVTDADDTSASGEAPAETPVQTPEAAAAAEAPASPGP
                     AAEPELVEPPVTPPEALAATETGTAGSDGEPTPPQAAR"
     gene            complement(1436..1996)
                     /locus_tag="PLQ18_07145"
     CDS             complement(1436..1996)
                     /locus_tag="PLQ18_07145"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007039688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HNE01467.1"
                     /translation="MGLWQRLGSVFKNDRQASQPLRRDPKVAVRVLMVCLGNLCRSPT
                     AEAVLAHLAVQAGLGRVIEIDSCGLSASNRGEPPDRRAREAAARRGYSMDDLRTRRFD
                     RDDLNYFEYVIAMDREVLETLQGYCMTPVQDAKLHLLLDYAPQLPEREVPDPYFGGRS
                     GFERVLDLIEPAATGLLAHLRREYRL"
     gene            2026..2295
                     /locus_tag="PLQ18_07150"
     CDS             2026..2295
                     /locus_tag="PLQ18_07150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE01468.1"
                     /translation="MPLPDTARLTGLIGRELDWLGQRCRIVELLPEGPSLVLEAVGAE
                     PSIQANQYGEATRRAVETHTVALLSPRGDALNPLLPGLPALLAAL"
     gene            complement(2292..2813)
                     /gene="kdsB"
                     /locus_tag="PLQ18_07155"
                     /pseudo
     CDS             complement(2292..2813)
                     /gene="kdsB"
                     /locus_tag="PLQ18_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004567493.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
     gene            complement(2916..3356)
                     /locus_tag="PLQ18_07160"
                     /pseudo
     CDS             complement(2916..3356)
                     /locus_tag="PLQ18_07160"
                     /inference="COORDINATES: protein motif:HMM:NF024486.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
BASE COUNT          522 a         1196 c         1213 g          476 t
ORIGIN      
        1 gagggcggtg acgcggcggc cgaagcgggc gccgcggtcg aggccggtgg cggtgaatcc
       61 ttcgcgtcaa ccgaaaccat cgaaaccacc gaaaccaccg aagctgtcga gtccgtcgaa
      121 accgcaccgg cgcgcagcag cttcgcggtg ccggccggtc gctacgtgcg caacggccgg
      181 ccgcgcctgc cgcgcggcac gctggccaac gccgatgccg tcaccggagc cccggagccg
      241 gtcaccggcc tggtgactgc cgccgtgacc gcggtcgccg tggccggcgc agcggaggcg
      301 gtggcgggcg aggcgccggt cacgaccgat gccgccgaaa cgaccgcagc ggagccaacg
      361 atggaagcgg ccgccggcag cgttccggtc gaaaccccgg cgccggtgcc cgaagccgcg
      421 gctccggtcg aggcgccggc ggcggccacc gatgcaccgg ccgaagccgc agtgcctgcc
      481 gaaaccgcgc cggtcgcgga agatgcgccg gtgccggagg atgcgccggt gccggtcgtc
      541 gacggcgaca tcgccgaggc cgtggccgaa gcggttgccg acatcctcgg cgaggcgcag
      601 gtcgctgccg gtgccatcgc cgatatgccg gccgaagcgc cggagacgcc agccgaggtc
      661 atgggcgatg tcgaggccgg cgacacggtg gtcgaagctg ccgtagccgt aaccgaagcg
      721 ccggtcccgg aaatggccgg ggcgacggac acgccggcca tcgaggccgc gccggacggg
      781 cagcctgaag tcgtcgtgcc gccgctgccg gtcgatgcgc cgcagcccgt gctgcccgag
      841 gcaccggtca cgacgccgga ggtgctggcc ggtaccgaag cggccgcaac gacggtgacg
      901 gacgcggacg acaccagcgc gtcgggcgaa gcgccggctg aaacgccggt tcagacaccc
      961 gaagcggctg cagcggccga ggccccggca agccccggac cggcagcaga gcctgagctt
     1021 gtcgagccgc cggtcacgcc gccggaagcg ctggctgcga ccgaaaccgg cacggccggc
     1081 agcgacggcg agccgacccc gccgcaggcg gcgcgctgac gcagcagccc gcggagcgcc
     1141 agcaacaaag aaggggcgcc ggactttccg gcgccccttc gtgctgtctg cgcggctcag
     1201 cctcaggatg tgccggcgca ggaggcgcac cgctcgcgaa atccgggccg agatcgagat
     1261 tcccgtgcct tccagcgctt tgcgcgaacg atgtgctttg ttcctcagcg catcctgcgt
     1321 gctgccggcc cgggcaaatc catggattcc tcggatcccg tgacatgcct cggcaaggca
     1381 cgacacgcaa agccggcacc ggcaccgctt tcgctcggcc gcggcatgac cggactcaga
     1441 gccggtattc gcggcgcagg tgggcgagca ggccggttgc ggccggttcg atcaggtcga
     1501 gcacgcgctc gaagccgctg cgtccgccga agtacgggtc cggcacctcg cgctcgggca
     1561 gttgcggggc gtagtcgagc agcaagtgca gcttcgcgtc ctggaccggc gtcatgcagt
     1621 agccctgcag ggtttccagc acctcgcggt ccatcgcgat cacgtattcg aagtagttca
     1681 ggtcgtcgcg atcgaagcgg cgcgtgcgca ggtcgtccat cgagtagccg cgccgggcgg
     1741 cggcctcgcg cgcgcgccgg tccggtggtt cgccgcggtt gctggccgac agtccgcagg
     1801 agtcgatctc gatgacccgg ccgagcccgg cctgcacggc gaggtgcgcg agcacggcct
     1861 cggcggtcgg cgagcggcac agattgccga gacagaccat cagcacgcgc accgcgacct
     1921 tcggatcgcg ccgcagcggc tgcgaggcct gacggtcgtt cttgaacaca ctgccgaggc
     1981 gttgccacaa tcccatcacc gtcctcctgc cgccaggcac ctccgatgcc cctgcccgat
     2041 actgcccgac tcaccggcct gatcggccgc gaactcgact ggctcggcca gcgttgccgg
     2101 atcgtcgagc tgctgcccga aggtccgtcg ctggtgctcg aagccgtcgg cgccgagccg
     2161 tcgatccagg ccaaccagta tggcgaggcc acgcgccgcg ccgtcgagac ccatacggta
     2221 gcgctgctca gtccgcgcgg cgatgcgctg aacccgctgc tgcccggcct gccggcgctg
     2281 ctcgccgcgc tctagccgcc gagcgcggct tcggcacggc gcaggtcctc ggccgtatcg
     2341 acgccggccg gcggtgccgg gtcggtaaac agcacgcgga tcgcgatgcc gtgccacagc
     2401 gcccgcagct gctccagcgc ttcggcgcgc tccagcggcg acggtgccag tcgcggatac
     2461 gcgcgcagca ccccggcgcg atacgcatac aggccgatgt gccgccagta cggcagcgcc
     2521 gggtccggct cgcgttcacc gcgcgcgaag cggtcgcggt cccaggggat cgtggcgcgg
     2581 ctgaagtaca gcgcccggcc atcggcggcg cagaccacct tcacggcgtt cggatcgaac
     2641 agttcgtgcg cggcgagcgg cgcggcgagc gtcgcgatgc cggcgagcgc gtcggcggcg
     2701 agtgcttcgg cggtctggcg caccagtgcc ggcggcagca gcggttcgtc gccctggacg
     2761 ttgacgacga tcgcctcgtc cggccagccg cgttgttctg caacttcggc gaggtcagac
     2821 tcccgctgcg ccgggttggc tgtccgctcg atgttcgcga gctgcaactt cgcttcgtcg
     2881 atgtgttcct cgagtcgcat ctcgctgaag cggagatcag ccagctcctg caaggacaga
     2941 gcttgccgaa ctgtctcccg aggtacgccg tgaatcgaac accactcatc gaggaacgcc
     3001 tggctcctga ctgtgacgat ctcgcgccat gcctccatct ggttgtccag caaaaagggc
     3061 tgagcgtccg cggtcacgcg agtcgctttg gcgtcggcca gtctgatcag ccgtgcgctg
     3121 tcgttgatcc gggcaatccg gagcaacgcg ttatcgaggg cgacgttcgt ctgcgacgca
     3181 ataaggatca gcgcacgtgg attccgctcg agctcctgag cgacaagctc ggcgatgaag
     3241 gtggtcttac ctgttccggg cggaccctgt acaaggaagg catcctgaca accgagcgct
     3301 gacgccactg cgtgctgttt gtcgaggtca aggtcaagct ggaaccaccg gtcgatttcc
     3361 gctgggtccg gcggttggct agtgctcggg tcgatgatga ggccacg
//
LOCUS       DAOQJH010000271         3396 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_17978_length_3396_cov_3.096678, whole genome shotgun sequence.
ACCESSION   DAOQJH010000271 DAOQJH010000000
VERSION     DAOQJH010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3396)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3396)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3396
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17978_length_3396_cov_3.096678"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1141
                     /locus_tag="PLQ18_07165"
     CDS             <1..1141
                     /locus_tag="PLQ18_07165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNE01469.1"
                     /translation="GLGLGGLTKGPAVLVYLLPPALLAPLWIVEARPDWRRWYRALGL
                     SLLGGFALALAWAVPAAIAGGAEYRGAILWGQTAGRMVQAFAHQRPFWWYLPVLPLIL
                     LPWTLWLPLWRALPALRTEAGGSALRFCLACFVPGFLIFSAVSGKQVHYLLPLLPALL
                     LPAARALTLGACDRRPGRLAVALVIAPVVGLGVLLALAPSFVDHYRWPDWVGTVSPLW
                     GLAVAGLGLAWAPLLRTRRAGLWLAVLPALLGTLLYAGIARPATAALDGSPVAQAIGR
                     LQAEGVTVAHPGKYHGEFQFPGRLRQPLEVITRGSELEWARAHPQGALIVRHDELDPE
                     QAALAVIVQPYRQRYAALWRAQTLIEHPGLLAKESGESRGSEGEE"
     gene            1216..2259
                     /gene="dusA"
                     /locus_tag="PLQ18_07170"
     CDS             1216..2259
                     /gene="dusA"
                     /locus_tag="PLQ18_07170"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015281088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HNE01470.1"
                     /translation="MPPAAGAPHDPLAPARRLSVAPMLDWTTRDFRYFARLLSQRVLL
                     YTEMVTTGAILHGDPARHLGFDAAEHPLALQLGGSDPQALAACARLAVDDFGYDEINL
                     NCGCPSDRVQEGRFGACLMAEPPLVADCVAAMRAAVPAHVPVTVKTRIGIDELDRYEQ
                     LLHFVGTVAAGGCTVFVIHARKAWLSGLSPKENREIPPLRYEVAAQLKRDCPQLSISL
                     NGGVQTLAQARQHLEWADGVMIGRAAYENPWLLAGADAALWGDTPMPRSRHDVLAAFE
                     PYVERHLARGGRLAHLTRHLLGLFQGVPGARAWRQYLSTHAHRHGAGLDVLRAAAAKV
                     SREVTQRYVSDRT"
     gene            2414..2671
                     /locus_tag="PLQ18_07175"
     CDS             2414..2671
                     /locus_tag="PLQ18_07175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE01471.1"
                     /translation="MDPRINASTEDQLIARIRTLTPQQVAEVEDFVEFLAARAGRRSA
                     LNRLLDIAPALETAGAEPIREDEIAAEIEAARAERRTRQPS"
     gene            2698..2994
                     /locus_tag="PLQ18_07180"
     CDS             2698..2994
                     /locus_tag="PLQ18_07180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE01472.1"
                     /translation="MLSALLWRGTPYHLLSAISQRPELRIYSSTALPEKLADLLARPT
                     ANRRLTLIGRSAGAVLADYVEAIVLPIPAACRAWWPAMSVTIRSLPPQSRRAPT"
BASE COUNT          473 a         1271 c         1171 g          481 t
ORIGIN      
        1 cggcctcgga ctcggcgggc tgaccaaggg accggccgtg ctcgtctacc tgctgccgcc
       61 ggcactgctc gccccgctgt ggatcgtcga ggcccgcccc gactggcggc gctggtatcg
      121 cgcactcggc ctgtcgctgc tcggcggctt cgcgctcgcg ctcgcctggg cggtgccggc
      181 ggcgatcgcc ggcggtgccg agtaccgcgg cgcgatcctg tgggggcaga cggccggacg
      241 catggtgcag gctttcgcgc atcagcggcc gttctggtgg tatctgccgg tgctgccgct
      301 gatcctgctg ccgtggacac tatggctgcc gctgtggcgc gcgctgccgg cgctgaggac
      361 cgaagccggc ggcagcgccc tgcgcttctg cctcgcctgc ttcgtgcccg gcttcctgat
      421 cttctcggcg gtcagcggca agcaggtgca ttacctgctg ccgctgctgc cggcgctgct
      481 gctgccggca gcgcgcgcgc tgacactcgg tgcctgcgat cgccggcccg gacgcctcgc
      541 ggtcgcgctc gtcatcgcgc cggtcgtcgg actcggcgtg ctgctcgcgc tggcgccgtc
      601 cttcgtcgat cactaccgct ggccggactg ggtcggcacg gtgtcgccgc tctggggcct
      661 tgcggtggca gggctcggtc tggcctgggc accgctgctg cgcacgcgcc gggccgggct
      721 gtggctcgcg gtcctgccgg ccctgctcgg cacgctgctg tatgccggca tcgcccggcc
      781 ggcgaccgcc gcactcgacg gcagcccggt ggcgcaggcg atcggccggc tgcaggccga
      841 aggcgtcacg gtcgcgcatc ccggcaagta ccacggcgaa ttccagttcc cgggccggct
      901 gaggcagcca ctcgaagtca tcacgcgcgg cagcgagctc gaatgggcgc gcgcgcaccc
      961 gcagggcgcg ctgatcgtgc gccacgacga gctcgatccg gagcaggccg cgctcgcggt
     1021 catcgtccag ccctaccgcc agcgctacgc ggccctctgg cgcgcgcaga cgctgatcga
     1081 gcaccccgga ctgctggcga aggaaagcgg cgagagccgt ggcagcgagg gcgaggaatg
     1141 atgaccggcc cgctcgcagc cgccccggat gccgcagcac cgcagcagga tcggagcccg
     1201 cgcaacggcg ccggcatgcc gcccgctgcc ggcgcgccgc atgacccgct cgcccctgcc
     1261 cgccgcctgt cggtcgcgcc gatgctcgac tggacgacgc gcgacttccg ttacttcgcg
     1321 cgcctgctgt cgcagcgcgt gctgctgtac accgagatgg tcacgaccgg cgccatcctg
     1381 cacggcgacc cggcccggca cctcggcttc gacgccgccg aacacccgct ggcgctgcag
     1441 ctcggcggct ccgacccgca ggccctcgcc gcctgcgccc gcctcgcggt cgatgacttc
     1501 ggctacgacg agatcaacct gaactgcggc tgcccgtcgg accgcgtgca ggaagggcgc
     1561 ttcggcgcct gcctgatggc cgagccgcca ctcgtcgccg actgcgtggc cgcgatgcgc
     1621 gccgcggtgc cggcacacgt gccggtgacc gtgaagacgc gcatcggcat cgacgagctc
     1681 gaccgctacg agcagctgct gcacttcgtc gggaccgtcg ccgccggcgg ctgcacggtg
     1741 ttcgtgatcc acgcgcgcaa ggcctggctc tcgggcctgt cgccgaagga aaaccgcgag
     1801 atcccgccgc tgcgctacga ggtcgccgcg cagctcaagc gcgactgccc gcagctttcg
     1861 atcagcctca acggcggcgt gcagacgctg gcgcaggccc gccagcacct cgaatgggcc
     1921 gacggcgtca tgatcggccg cgccgcctac gagaacccct ggctgctcgc cggagccgat
     1981 gcggccctct ggggcgatac cccgatgccg cgcagccgcc acgacgtgct cgccgccttc
     2041 gaaccctacg tcgaacggca tctggcccgc ggcggacgac tcgcacacct cacgcgccac
     2101 ctgctcggtc tgttccaggg cgtgcccggt gcgcgcgcgt ggcggcaata tctatcgacg
     2161 cacgcgcacc ggcacggcgc cggcctcgac gtgctgcgcg cggcagcggc gaaggtgtcg
     2221 cgcgaagtga cgcaacggta cgtgtcagac aggacctgaa cgccgaccac gcctggccgc
     2281 ggcggcgcat cgggcaagcc gacggcctgt agccgtcggc ttgcgctcga caaatccggc
     2341 accacagccc gatcctgata acggcagaaa accgctgcgg gcacgggcgt accgcccctc
     2401 cggagaccca agaatggacc ccagaatcaa tgcgagcact gaagatcaac tgatcgccag
     2461 gatcaggacg ctgactccgc aacaggtggc ggaagtggaa gacttcgtgg aattcctcgc
     2521 tgcccgcgcc gggaggcgca gcgcactgaa ccggttgctg gacatcgccc cggcgctgga
     2581 aaccgccgga gccgaaccga tccgtgagga cgagatcgct gccgaaatcg aggcggcccg
     2641 cgccgagcgt cgcacccggc aaccgtcctg atgcggatcg tcttggatac cagcgtcgtg
     2701 ctgtcggcgc tgctgtggcg cggcacgccg taccacttgc tgagcgccat cagccagcgt
     2761 cccgaactgc ggatctacag cagcacggcg ctgcccgaaa aactcgccga cctgctggcc
     2821 cgccccaccg cgaaccggcg actgaccctg atcggcagat cagccggcgc tgtgctggcc
     2881 gattacgtcg aggccatcgt gctgccgatc ccggcagcgt gccgcgcgtg gtggccggcg
     2941 atgtcggtga cgatcaggtc attgccgccg cagtcgcggc gtgcgccgac ctgatcgtca
     3001 ccggggatcg caagcatctg ctgccgctcg gcagccatca gggtattgcg atcgtcgaag
     3061 ccgccgaagc gctccggcag atcaccggca cctgatcgca gcccctgcaa ctgcaagccc
     3121 cggcgtccgg gagggatgcc tgcgcgccag cggctgatcc ctgcgcggca tggacggggc
     3181 cttgcgcatc atcggcagac cctcgcacgg catgggccgc cccttgcacg tcatcggcca
     3241 atccttgcgc agcagacccg gacgcccctg aaacagccgc ggcccgcccg gtttcccgga
     3301 cgggccgcat ctgagcatcg ggcgggccga agcccgccgg ttcaggccgc cagcgtcagc
     3361 agcaggccgt tcaggcgctt gacgaaggtc gccggg
//
LOCUS       DAOQJH010000272         3390 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_18023_length_3390_cov_4.429985, whole genome shotgun sequence.
ACCESSION   DAOQJH010000272 DAOQJH010000000
VERSION     DAOQJH010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3390
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18023_length_3390_cov_4.429985"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..401)
                     /gene="dtd"
                     /locus_tag="PLQ18_07185"
     CDS             complement(<1..401)
                     /gene="dtd"
                     /locus_tag="PLQ18_07185"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019569284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HNE01473.1"
                     /translation="MIGLLQRVTRAQVTVAGEPVGAIAAGLLVLVGVERGDGEAQAAR
                     LLERLLGYRVFADADGRMNRSLADTGGGLLLVPQFTLPADTQKGTRPSFTPAAAPADG
                     ERLFRHLLGLAEAAHAPVASGRFGADMQVELV"
     gene            complement(398..1348)
                     /gene="pip"
                     /locus_tag="PLQ18_07190"
     CDS             complement(398..1348)
                     /gene="pip"
                     /locus_tag="PLQ18_07190"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HNE01474.1"
                     /translation="MRELYPDIRPYATRRLPVSPLHTLYVEETGTPGGLPVLFLHGGP
                     GAGCQPVHRRFFDPLRYRIVLFDQRGAGQSTPHAELTDNTTTALIADIELLRKTLGIE
                     RWVVFGGSWGSTLALAYAEAHPERVLGLVLRGIFLCRPQDIRWFYQSGAGRLFPEAWE
                     DYLAPIPETERGDLVSAYYRRLTGDDEVERMRCAKAWSVWEGTTLTLRPSPHTVDHFA
                     DPHMALSLARIECHYFMHDSFLAPDQLVRDVSRLAGIPGYIVHGRYDVICPLDGAWAL
                     HRAWPGSQLDIIADAGHAATEPGIIDALVRATDALADHYR"
     gene            complement(1836..2957)
                     /gene="hemH"
                     /locus_tag="PLQ18_07195"
     CDS             complement(1836..2957)
                     /gene="hemH"
                     /locus_tag="PLQ18_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005224071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HNE01475.1"
                     /translation="MSGPDSRASAPVHVHGRAETTGILLVNLGTPDAPTPAALRRYLA
                     EFLWDRRVVEIPRPLWWLILHGIILRTRPAKSAAKYATVWTPEGSPLLAIGRRQQAGL
                     QARLAAGDHGPLQVALGMRYGQPSIATALAELRAAGCTRLLVLPLYPQYSAATTATAF
                     DALAAELARWRRLPELRCVMQYHDEPAWVDAVAASLDQHAAAHGGLAERVLFSFHGLP
                     KRNLALGDPYHCQCLKSARLIAGRLGLADDRWAVAFQSRFGRAEWLRPYTSETLQQWA
                     RTGVRSVDVICPGFAADCLETLEEIAIENRLLFEQAGGERLRYVPALNDAPAHLDALA
                     QLVRRHCSGWPGFAGGPAPHAAAELAASRERALACGADD"
     gene            3068..>3390
                     /locus_tag="PLQ18_07200"
     CDS             3068..>3390
                     /locus_tag="PLQ18_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007162725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate dehydrogenase"
                     /protein_id="HNE01476.1"
                     /translation="MHGVFLDRASLDLGDLDFSALTHALDRFTGYAATAPDEVAVRIR
                     GADIVIVNKVVLDAATLATAAALKLICVAATGTNNVDLEAARARGIPVVNCRGYGTPA
                     VAQHA"
BASE COUNT          498 a         1180 c         1264 g          448 t
ORIGIN      
        1 accagttcga cctgcatgtc ggcgccgaag cgcccgctcg cgaccggggc gtgcgcggct
       61 tcggcgagcc cgagcagatg ccggaacagc cgctcgccgt cggccggtgc cgccgccggc
      121 gtgaagctcg ggcgcgtgcc cttctgcgta tcggccggca gcgtgaactg cggcaccagc
      181 agcaggccgc cgccggtatc ggcgaggctg cggttcatgc gcccgtcggc atcggcgaag
      241 acgcggtagc cgagcaggcg ttcgagcagg cgcgcggcct gcgcctcgcc gtcgccgcgc
      301 tcgacgccga ccagcaccag cagcccggcc gcgatcgcac cgaccggctc gccggcgacc
      361 gtcacctgcg cgcgcgtgac gcgctgcagc agcccgatca ccggtagtgg tcggcgagtg
      421 cgtcggtggc gcgcaccagc gcgtcgatga tgccgggctc ggtcgccgcg tggccggcat
      481 cggcgatgat gtcgagctgc gagcccggcc aggcgcggtg cagcgcccag gcgccgtcga
      541 gcgggcagat cacgtcgtag cggccgtgga cgatgtagcc ggggatgccg gcgaggcggc
      601 tgacatcgcg caccagctgg tcgggcgcga ggaagctgtc gtgcatgaag tagtggcact
      661 cgatgcgcgc cagcgacagc gccatgtgcg gatcggcgaa gtgatcgacc gtgtgcggac
      721 tcgggcgcag cgtcagcgtc gtgccctccc agaccgacca ggccttggcg cagcgcatgc
      781 gctcgacctc gtcatcgccg gtcaggcgcc ggtagtaggc cgagaccaga tcgccgcgct
      841 cggtctccgg gatcggtgcc agatagtcct cccaggcctc cgggaacagt cgcccggccc
      901 cggactgata gaaccagcgg atgtcctgcg gccggcacag gaagatgccg cgcagcacca
      961 gcccgagcac gcgctccggg tgggcctcgg cgtaggcgag cgccagcgtc gagccccagg
     1021 agccgccgaa caccacccag cgctcgatgc cgagcgtctt gcgcagcagt tcgatgtcgg
     1081 cgatcagcgc cgtcgtggtg ttgtcggtca gctcggcgtg cggcgtgctc tgaccggcgc
     1141 cgcgctggtc gaacagcacg atgcgatagc gcagcggatc gaaaaaccgc cggtgcaccg
     1201 gctggcagcc ggccccgggg ccgccgtgca ggaacagcac cggcaggccg ccgggcgtgc
     1261 cggtttcctc gacgtacagc gtgtgcagcg ggctgaccgg caggcggcgc gtcgcatacg
     1321 gacggatgtc gggataaagc tcgcgcatgt cgcaggctcc gccggaagcg gtgccggcca
     1381 cgggtgaacc ggtcgtttcc gggataagaa atttctgatg gtcgattcag cggatggcgc
     1441 agccgaaacc gcctgatggc gcatggctgt catgaaacag ctgtatacac tgtgcgccca
     1501 tgcaggaggg aacgggaagc ggcagacagg ccgcgccgca ggaagggcgg cagcgcagga
     1561 ggcgcggcac ggaagcccga ggcaggtccg gggcatgaaa cggtgagggg aacaccgctt
     1621 tcgggcccgg atcgaggcgg ggctgggaca cggtgcggaa cgcaccgggc ggtgcacggg
     1681 gcaccacgtc agggaagacg gctcagggag agcgacgtgg aaggcgcggg tcgggatggc
     1741 ccgtaccgga agcggtgtat caccttctca atgtttggct gctacgtttt tcagccgccg
     1801 tcgcgcatgc gatggcggca tttttctttt ggcggtcagt catccgcgcc gcaggcgagc
     1861 gcccgctcgc gcgaggcggc gagctcggcc gccgcgtgcg gcgccggtcc gccggcaaag
     1921 cccggccagc cgctgcaatg gcggcggacc agctgcgcga gcgcgtcgag gtgcgccggc
     1981 gcgtcgttca gcgccggcac gtagcgcagc cgctcgccgc cggcctgctc gaacagcagg
     2041 cggttctcga tcgcgatctc ttcgagcgtc tccagacagt cggccgcgaa gcccgggcag
     2101 atcacgtcga cgctgcgcac gccggtgcgc gcccactgct gcagggtctc gctggtgtag
     2161 ggcctgagcc actcggcgcg cccgaagcgc gactggaagg ccacggccca gcgatcgtcg
     2221 gcgagcccga gccgcccggc gatcaggcgc gcgctcttca ggcactggca gtggtaggga
     2281 tcgccgagcg cgaggttgcg cttcggcagg ccgtggaacg agaacagcac gcgctcggcc
     2341 aggccgccgt gggcggcggc gtgctgatcg aggctcgcgg cgacggcgtc cacccaggcc
     2401 ggctcgtcgt ggtactgcat cacgcagcgc aactccggca gccggcgcca gcgcgcgagt
     2461 tcggcggcga gcgcatcgaa ggccgtggcc gtggtcgcgg ccgagtactg cggatacagc
     2521 ggcagcacca gcagccgcgt gcagccggcg gcgcgcagct cggcgagcgc cgtggcgatc
     2581 gacggctggc cgtagcgcat gccgagcgcg acctgcagcg ggccatggtc gccggccgcg
     2641 agccgcgcct gcagcccggc ctgctggcgg cggccgatcg cgagcagcgg cgagccttcc
     2701 ggcgtccaga ccgtcgcgta cttggccgcc gacttcgccg ggcgcgtgcg caggatgatg
     2761 ccgtgcagga tcagccacca cagcggccgc gggatctcga cgacgcgccg gtcccacagg
     2821 aactcggcga gatagcggcg cagtgccgcc ggcgtcgggg cgtccggcgt gccgaggttg
     2881 accagcagga tgccggtcgt ctcggcacgg ccgtggacgt gaacaggggc tgaagcgcgg
     2941 gagtccggtc cggacatgcg ggaggcttcc tcggagcgtg cgggaacggg cgcatcgatt
     3001 atattccggg tttgccggcg ccccgccggg cccgcgttcc ccccgttgcc cgcaggtatc
     3061 ccgccccatg cacggcgtct tcctcgaccg cgcctcgctc gatctcggcg atctcgattt
     3121 ctcggcgctg acccatgcgc tcgatcgctt caccggctac gccgccaccg cccccgacga
     3181 ggtcgccgtg cgcatccgcg gcgccgacat cgtcatcgtc aacaaggtcg tgcttgatgc
     3241 cgcgaccctc gccacggccg cggcgctgaa gctgatctgc gtcgccgcca ccggcaccaa
     3301 caacgtcgat ctcgaagcgg cgcgcgcgcg cggcatcccg gtcgtcaact gccgcggcta
     3361 cggcacgccg gcggtcgcgc agcacgcgat
//
LOCUS       DAOQJH010000273         3390 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_18027_length_3390_cov_3.643178, whole genome shotgun sequence.
ACCESSION   DAOQJH010000273 DAOQJH010000000
VERSION     DAOQJH010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3390
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18027_length_3390_cov_3.643178"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1333
                     /gene="urtB"
                     /locus_tag="PLQ18_07205"
     CDS             <1..1333
                     /gene="urtB"
                     /locus_tag="PLQ18_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002682742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtB"
                     /protein_id="HNE01477.1"
                     /translation="VNAADGQPWTGAAGDAPAMTDAVRRVLASSIERARLRSEDRAER
                     LGAARALNDRPSASAEPALRAALAEEQDPEVVTALKLALAPLDLDSPDKDKKLAALAV
                     LANDGELTVLGRLQPLASGDDPEIRAAAQKAITAIGDRQWLRDQGANLFYGLSLGSVL
                     LLAALGLAITFGLMGVINMAHGELLMIGAYATYVVQNLFRAYLPGAFDWYLLAALPVA
                     FLSAAAVGWALERVVLRHLYGRPLETLLATFGLSLILIQTVRLIFGAQNVEVANPSWL
                     SGGIQLTQQLVLPYNRIATILFALAVLVTVWLLFQKTRLGLRVRAVTQNRGMAAAMGI
                     DTRRVDAGTFALGAGVAGLAGVALSQLGNVGPELGQAYIVDSFMVVVLGGVGKLAGAV
                     AGGMGLGIVNKFLEPSMGAVLGKIALLGFIILFIQWRPQGLFAMKGRAVEG"
     gene            1338..2438
                     /gene="urtC"
                     /locus_tag="PLQ18_07210"
     CDS             1338..2438
                     /gene="urtC"
                     /locus_tag="PLQ18_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006579907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtC"
                     /protein_id="HNE01478.1"
                     /translation="MHGIRNLHTTRGWVGFFVVAALLLVAVPVLNALPETSPLHVPDW
                     LIPLAGKFCCYALVALAMDLIWGYTGILSLGHGVYFALGGYAMGMYLMRSMAGEGVYR
                     SELPDFMVFLNWKELPWFWHGFEYFPFALLMALAVPGLLAFVFGWFAFRSRIKGVYFS
                     IITQAMTFALMLLFFRNETGFGGNNGLTDFKRLLGFTLSEPSVRVALYWLTALVLLGV
                     YLLARAIVRSRAGRVLTAIRDAESRVMFCGYEPLYYKLAIWTLSAVLCGLAGALYVPQ
                     VGIINPSELAPAQSIEMAVWVAVGGRGTLVGAVIGAASVNGLKSVFTAVAPEFWLYAL
                     GGLFVLVTLFLPEGLTGLFRRLRRTRAAEARA"
     gene            2435..3334
                     /gene="urtD"
                     /locus_tag="PLQ18_07215"
     CDS             2435..3334
                     /gene="urtD"
                     /locus_tag="PLQ18_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004580898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter ATP-binding protein UrtD"
                     /protein_id="HNE01479.1"
                     /translation="MNPVIPPVTPAGDALKGAQMLHDASEAPPKPPAAPREPLPADVR
                     DRPVPDVSHGAILYLESVSVSFDGFKAINDLNLSIDDGELRCIIGPNGAGKTTMMDII
                     TGKTRPDTGTVYFGQNVNLLELRESEIAQLGIGRKFQKPTVFQGHSVFENLELAMQAD
                     KRVRRALFARLSDEQRARIAGVLATIGLNGYERQLAGSLSHGQKQWLEIGMLLMQNPR
                     LLLVDEPVAGMTEQETEQTAELLNSLAGKHSVVVVEHDMAFVRSIARKVTVLHEGHVL
                     AEGTMDAIQSNPKVVEVYLGEGA"
BASE COUNT          466 a         1159 c         1200 g          565 t
ORIGIN      
        1 ggtcaacgcc gccgacggtc agccgtggac cggtgccgcc ggtgatgcgc cggcgatgac
       61 cgacgccgtg cgccgcgtgc tcgccagttc catcgagcgc gcgcgcctgc gctccgagga
      121 ccgcgccgag cgcctcggtg ccgcgcgggc attgaacgac cggccatcgg cgagcgccga
      181 accggcgctg cgcgccgcac tcgccgagga gcaggacccg gaagtggtga cggcgctgaa
      241 gctcgcgctg gcgccgctcg atctcgacag tcccgacaag gacaagaagc tcgccgcact
      301 cgccgtgctc gccaatgacg gcgagctgac cgtgctcggc cggctgcagc cgctcgcgag
      361 cggcgatgac cccgaaatcc gcgctgcggc gcagaaggcg atcacggcca tcggtgaccg
      421 ccagtggctg cgcgaccagg gcgccaacct gttctacggc ctgtcgctcg gcagcgtgct
      481 gctgctcgcc gcgctcgggc tggcgatcac cttcgggctg atgggcgtca tcaacatggc
      541 gcacggcgag ctgctgatga tcggcgccta cgcgacctac gtcgtgcaga acctgttccg
      601 cgcctacctg cccggggcct tcgactggta tctgctcgcg gcgctgccgg tggccttcct
      661 gagcgcggcg gcggtcggct gggcgctgga gcgcgtcgtg ctgcgccacc tgtacggccg
      721 gccgctcgaa accctgctcg cgaccttcgg cctgtcgctg atcctgatcc agaccgtgcg
      781 cctgatcttc ggtgcgcaga acgtcgaggt cgccaatccc tcgtggctgt cgggcggcat
      841 ccagctcacg cagcagctgg tgctgccgta caaccgcatc gcgacgatcc tgttcgcgct
      901 ggcggtgctg gtgacggtct ggctgctgtt ccagaagacc cggctcggcc tgcgcgtgcg
      961 cgcggtgacg cagaaccgcg gcatggccgc ggcgatgggc atcgacacgc gccgcgtcga
     1021 tgccggcacc ttcgcgctcg gcgccggcgt ggcggggctc gccggcgtgg cgctgtcgca
     1081 gctcggcaac gtcggcccgg agctcgggca ggcctacatc gtcgattcct tcatggtcgt
     1141 ggtgctcggc ggcgtcggca agctcgccgg cgcggtcgcc ggcggcatgg ggctcggcat
     1201 cgtcaacaag ttcctggagc cgtcgatggg cgcggtgctc ggcaagatcg cgctgctcgg
     1261 cttcatcatc ctgttcatcc agtggcggcc gcaggggctg ttcgcgatga agggccgggc
     1321 ggtggagggc tgaaccgatg cacggcatcc gcaatctgca tacgacccgc ggctgggtcg
     1381 gcttcttcgt ggtcgcggca ctgctgctgg tggcggtgcc ggtgctcaac gcactgcccg
     1441 agaccagtcc gctgcacgtg cccgactggc tgatcccgct cgccggcaag ttctgctgct
     1501 acgcgctggt ggcgctggcg atggacctga tctggggcta caccggcatc ctgtcgctcg
     1561 gccacggcgt gtacttcgcg ctcggcggct acgcgatggg catgtacctg atgcgcagca
     1621 tggccggcga gggcgtctac cgctcggagc tgccggactt catggtgttc ctgaactgga
     1681 aggagctgcc gtggttctgg cacggcttcg agtacttccc gttcgcactg ctgatggcgc
     1741 tcgccgtgcc ggggctgctc gccttcgtgt tcggctggtt cgcgttccgc tcgcgcatca
     1801 agggcgtgta cttctcgatc atcacgcagg cgatgacctt cgcgctgatg ctgctgttct
     1861 tccgcaacga gaccggcttc ggcggcaaca acggcctgac cgacttcaag cgcctgctcg
     1921 gcttcacgct cagcgagccc tcggtgcgcg tggcgctgta ctggctgacg gcgctggtgc
     1981 tgctcggcgt gtatctgctc gcccgcgcga tcgtgcgctc gcgtgccggg cgcgtgctga
     2041 ccgcgatccg cgacgccgag agccgcgtga tgttctgcgg ctacgagccg ctgtactaca
     2101 agctcgcgat ctggacgctg tcggcggtgc tctgcggtct ggccggtgcg ctgtacgtgc
     2161 cgcaggtcgg catcatcaac ccgagcgagc tggcgccggc gcagtcgatc gagatggctg
     2221 tctgggtcgc ggtcggcggg cgcggcacgc tggtcggggc cgtgatcggc gcggccagcg
     2281 tcaacggcct caagagcgtg ttcacggccg tggcgccgga gttctggctg tacgcgctcg
     2341 gcggcctgtt cgtgctggtg acgctgttcc tgcccgaggg cctgaccggc ctgttccggc
     2401 gcctgcgccg cacccgagcc gcggaggccc gcgcatgaat cccgtgatcc cgcccgtcac
     2461 ccctgccggt gatgccctga agggcgcgca gatgctgcac gacgcctccg aagcgccgcc
     2521 gaaaccgccg gctgcgccac gcgagccgct gccggccgac gtgcgcgacc ggccggtgcc
     2581 ggacgtttcg cacggcgcga tcctgtacct cgaaagcgtc agcgtcagct tcgacggctt
     2641 caaggcgatc aacgacctga acctgtcgat cgacgacggc gagctgcgct gcatcatcgg
     2701 cccgaacggc gccggcaaga cgacgatgat ggacatcatc accggcaaga cccgtccgga
     2761 caccggcacc gtgtacttcg gccagaacgt caacctgctc gaactgcgcg agtccgagat
     2821 cgcgcagctc ggcatcggcc gcaagttcca gaagccgacc gtgttccagg gccactcggt
     2881 gttcgagaac ctcgaactgg cgatgcaggc cgacaagcgc gtgcgccggg cgctgttcgc
     2941 gcgcctgagc gacgagcagc gcgcacgcat cgccggggtg ctcgcgacca tcggcctcaa
     3001 cggctacgag cgccagctcg ccggcagcct gtcgcacggt cagaagcagt ggctggagat
     3061 cggcatgctg ctgatgcaga acccgcgcct gctgctggtc gacgagccgg tcgccggcat
     3121 gaccgagcag gagaccgagc agaccgccga gctgctgaac tcgctggccg gaaagcattc
     3181 ggtcgtcgtc gtcgagcacg acatggcctt cgtgcgttcg atcgcgcgca aggtcacggt
     3241 gctgcacgag ggccacgtgc tcgccgaggg cacgatggac gcgatccaga gcaatccgaa
     3301 ggtggtcgag gtgtatctcg gggagggggc gtgatggcgg cggtgctgca ggaatccacg
     3361 gcggccgatg cgctgctgac ggtcgatcag
//
LOCUS       DAOQJH010000274         3387 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_18044_length_3387_cov_4.383253, whole genome shotgun sequence.
ACCESSION   DAOQJH010000274 DAOQJH010000000
VERSION     DAOQJH010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3387
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18044_length_3387_cov_4.383253"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..586)
                     /locus_tag="PLQ18_07220"
     CDS             complement(<1..586)
                     /locus_tag="PLQ18_07220"
                     /inference="COORDINATES: protein motif:HMM:NF015549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HNE01480.1"
                     /translation="MPSPDVLAAALRTCLPVAFGYVPLGMAFGVLLVQSGQPAWLAPL
                     MGLCIYAGAAQFLAVGLLAARVPLADIALATLLLNLRHAFYGLSMIDRLPVRGWRRWY
                     AVFALTDETWSLLTGTRPPPAFATPPAAHDFVFAVSALNQGWWLLGCTLGGLGGLALG
                     GLDTHGLEFTLTALFVVLLVEQWHAVREPWPFLLA"
     gene            1127..1567
                     /locus_tag="PLQ18_07225"
     CDS             1127..1567
                     /locus_tag="PLQ18_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE01481.1"
                     /translation="MNNKLMACLLAATLALPLSAPAQAGSGDALIGGGVGGALGAVVG
                     HEISGRDGAIVGGALGAALGAAATTQGDRDYRYRRVYNGPVYERPYVVERPYYAPPPP
                     RYYAPPPRYVVERRVYVEDRGYRRGPPPWARHHHRHHRHWRDWD"
     gene            1753..>3387
                     /locus_tag="PLQ18_07230"
     CDS             1753..>3387
                     /locus_tag="PLQ18_07230"
                     /inference="COORDINATES: protein motif:HMM:NF023459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="von Willebrand factor type A domain-containing
                     protein"
                     /protein_id="HNE01482.1"
                     /translation="MSAVPRLRAAGLVLIGLTATLVSACGGGGGPYAGAPVAGLPPPK
                     VTGALAGGMSGALLAKATEPRGAPAALPAAQFPQAGKSPVVRTAEQPVSTFSLDVDTA
                     SYALVRRLLNDGRLPPVDAVRIEEMVNYFPYAWPAPEARSRPLQVSTALLPTPWNPDT
                     RLLRIGIRGWAPATHERPPANLVFLIDVSGSMDAPDRLGLLQKSLGLLVERLRPEDRV
                     AIVSYAGGVRTVLEPTSGRERTKILAALDGLAAGGSTAGAAGLEQAYRLAREHFDRQA
                     VNRVILGTDGDFNVGLSDPAGLAQYVSEQRKAGVYLSVLTVGLDNLHDGTAQALAQAG
                     NGNAAYLDSVQEGRRVLVEQLAATMIPVADDAKVQVEFNPAEVSEYRLLGYETRALAR
                     ADFTNDAVDAGDLGVGHTVTALYEITPAGARARIEPGRYEAPAATVPNRHPGELAWVK
                     LRWKQPGAARSEALAQPVRAADAFAGIAAADADTRFAVAVAAFGQWLRQDTELRGYGI
                     DAIETLAQGARGEDGEGRRAEFVQLVRQAAALRGAPA"
BASE COUNT          496 a         1217 c         1204 g          470 t
ORIGIN      
        1 cggcgagcag gaacggccac ggctcgcgca ccgcgtgcca ctgctcgacc agcagcacga
       61 cgaacagcgc ggtcagcgtg aattcgagcc cgtgcgtgtc gagcccgccg agcgcgagtc
      121 cgccgagccc gccgagcgtg cagccgagca gccaccagcc ctggttcagc gcgctgaccg
      181 cgaacacgaa atcgtgcgcg gccggcgggg tggcgaaggc cggtggcggg cgcgtgccgg
      241 tcagcagcga ccaggtttcg tcggtcagcg cgaacaccgc ataccagcgc cgccagccgc
      301 gcaccggcag ccggtcgatc atcgacaggc catagaaggc atgacgcagg ttcagcagca
      361 gcgtcgcgag tgcgatgtcg gcgagcggca cccgggccgc gagcaggccg accgcgagga
      421 actgcgcggc cccggcgtag atgcagagcc ccatcagcgg ggccagccag gccggctggc
      481 cgctctgcac cagcagcacg ccgaacgcca tcccgagcgg cacatagccg aacgccaccg
      541 gcagacaggt gcgcagggcg gcagcgagca cgtcggggct gggcatgtgc ggcatccgga
      601 atgggggcga tccaaagtac cggatagtaa ctaaatacat ggctatcgtg tgggaatacc
      661 gcagtgccgc aacgcgcgtt cctgccgcgc atcccggccc tctgcggcgc ggagcattcg
      721 attgcgggct tgcgaccatg cagttatttt gaaatccggt ctgaccgcgc ctccctgccg
      781 cgttggccgc ccgcaacagt caaatcgcgg atcgtcgctg caaggcgaca atgcaagtcc
      841 ctgacggata tctgatcttt tccgcatccg gtccgctcgt cgtcgaaaag cgacgaatct
      901 ccccgtgaat tccgcgatgt ccaggccgca tggcccggtt ttttctggaa atttcggtgt
      961 tggcactacg attgctttgg ctgatgccaa gggaggcgcg agtcgcggtc cgggtttaag
     1021 aacggccccg tccccgatcc ggccgcggct cgcgcacccc tcctgacgtg acagccgtcg
     1081 gcagcggtac tgccgcaatt cgtctcctcc agaggagtca aaaatcatga acaacaaact
     1141 catggcctgc ctgctggcgg cgacgctggc gctgccgctg agcgcgccgg cgcaggccgg
     1201 cagcggcgat gcgctgatcg gcggtggcgt cggtggtgcg ctcggtgcgg tggtcggcca
     1261 cgagatcagc ggtcgcgacg gcgcgatcgt cggcggcgcg ctcggcgcgg cgctcggcgc
     1321 ggcggccacg acgcagggtg atcgcgatta ccgttaccgg cgcgtctaca acgggccggt
     1381 ctacgaacgt ccgtatgtcg tcgagcggcc gtactacgcc ccgccgccgc cgcgctatta
     1441 cgcgccgccg ccgcgctacg tcgtcgagcg ccgcgtctac gtcgaggatc gcggctatcg
     1501 ccgcggcccg ccgccgtggg cccggcacca ccatcgccat caccggcact ggcgcgactg
     1561 ggactgaata cccgctgagc gctgccggtt ctctgcgacg ccgcccccag actccgggcg
     1621 gcgttttttt gtgcgtgcgt cgcgccggcc gaccacactg cagacccccg cccgcatcgc
     1681 cgtcttctgc aagctgcctg cgcacccttc ttccgcaacc tccatccgca acctccatcc
     1741 gggagtcgtt ccatgtctgc cgtcccgcgc ctgcgcgccg ccggtcttgt gctgatcggc
     1801 ctgaccgcca cccttgtctc ggcctgcggg ggcggcggcg gtccgtacgc cggcgcaccg
     1861 gtcgccggcc tgccgccgcc gaaggtgacg ggcgcactgg cgggcgggat gtcgggggcg
     1921 ctgctggcga aggccaccga accgcgcggc gcaccggcgg cgctgccggc cgcgcagttc
     1981 ccgcaggccg gcaagagccc ggtggtgcgc acggccgaac agccggtctc gaccttcagc
     2041 ctcgatgtcg acacggcctc gtatgcgctg gtgcgccggc tgctcaacga cggccggctg
     2101 ccgccggtcg atgcggtgcg catcgaggag atggtcaact acttcccgta cgcgtggccg
     2161 gcgcccgagg cccgcagccg gccgctgcag gtgtcgacgg cgctgctgcc gacgccgtgg
     2221 aacccggaca cgcgcctgct gcgcatcggc atccggggct gggcgccggc cacgcacgag
     2281 cggccgccgg cgaatctggt gttcctgatc gacgtgtccg gctcgatgga cgcgcccgac
     2341 cgcctcgggc tgctgcagaa gtcgctcggc ctgctggtcg agcggctgcg gcccgaggac
     2401 cgcgtcgcga tcgtcagcta cgccggaggc gtgcgcacgg tgctggagcc gacctccgga
     2461 cgcgagcgca cgaagatcct cgccgcgctc gatggtctcg ccgccggcgg cagcacggcc
     2521 ggggcggccg ggctggagca ggcctaccgg ctggcgcgcg agcacttcga ccggcaggcg
     2581 gtcaaccgcg tgatcctcgg caccgacggc gacttcaacg tcggcctgtc cgatccggcc
     2641 gggctcgcgc agtacgtgag cgagcagcgc aaggcgggcg tgtacctgtc ggtgctgacc
     2701 gtcggtctcg acaacctgca tgacggcacg gcgcaggcgc tggcgcaggc cggcaacggc
     2761 aatgccgcct atctcgacag cgtccaggaa ggccggcgcg tgctggtcga gcagctcgcg
     2821 gcgacgatga ttccggttgc cgacgatgcc aaggtgcagg tcgagttcaa cccggccgag
     2881 gtcagcgaat accggctgct cggctacgag acgcgcgccc tggcgcgggc cgacttcacg
     2941 aacgatgccg tcgatgccgg tgacctcggc gtcggccaca cggtcacggc gctctacgag
     3001 atcacgccgg ccggcgcccg ggcgcgcatc gagcccgggc gctacgaggc accggcagcg
     3061 acggtgccga accgccatcc cggcgaactc gcctgggtga agctgcgctg gaagcagccg
     3121 ggcgcggcgc gcagcgaagc cctcgcgcag ccggtgcgtg cggccgacgc cttcgccggc
     3181 atcgccgccg ccgatgccga cacgcgcttc gcggtcgcgg tcgccgcctt cggacagtgg
     3241 ctgcgccagg acacggagct gcgcggctac ggcatcgacg cgatcgaaac cctggcgcag
     3301 ggcgcacgcg gcgaggatgg cgagggccgg cgggccgagt tcgtgcagct cgtgcgccag
     3361 gccgcggcac tgcgcggcgc gccggcg
//
LOCUS       DAOQJH010000275         3380 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_18108_length_3380_cov_3.626767, whole genome shotgun sequence.
ACCESSION   DAOQJH010000275 DAOQJH010000000
VERSION     DAOQJH010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3380)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3380)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3380
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18108_length_3380_cov_3.626767"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..708)
                     /locus_tag="PLQ18_07235"
     CDS             complement(<1..708)
                     /locus_tag="PLQ18_07235"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNE01483.1"
                     /translation="MTDLAAPAAPDRLSEAIELHRRGQGLAAEPIYLELLAADAGHPD
                     ALHYLGLLRHQQGRDDEALDLIGRALQAVPRYTDAWNNLGNVHLRAGRFDEAETAYRA
                     ALECDAGHAGAWNNLGLTLRARGLYADSAEAYAKLASLAPDFAEAHYQLGLMLRHTST
                     LASVLDAFRRCIEIDPTHARAHYQYGYMLYITGHTAEAADVFRQWLTFDAANPVPRHM
                     LAACSGEDVPARAADDYV"
     gene            complement(833..1249)
                     /locus_tag="PLQ18_07240"
     CDS             complement(833..1249)
                     /locus_tag="PLQ18_07240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE01484.1"
                     /translation="MKPVRALLAAVLLAAGTVAGAGGEMPAGKRVAPPAVAPLTLHGV
                     RYEAPPDTRDFGDDQAGGYLVARDARSGRELWRQRIYATAYDPKLESDVQDVFIASLR
                     AGRGGRTLEITDERGRRYTLDLGSRAVQEQGVQNRR"
     gene            complement(1246..1596)
                     /locus_tag="PLQ18_07245"
     CDS             complement(1246..1596)
                     /locus_tag="PLQ18_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013817568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE01485.1"
                     /translation="MNDDELEAGLPAPKRVGPPEVEPVTIGNVRYEVVHWGRERGLGQ
                     NGGYLEAFDAASGASLWLLQVYVIDYDPDLEEDVQDVFIESLKTGPRGTLKIVDERGR
                     RFVVDPATRTVTAK"
     gene            complement(1624..2637)
                     /locus_tag="PLQ18_07250"
     CDS             complement(1624..2637)
                     /locus_tag="PLQ18_07250"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020157154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M35 family metallo-endopeptidase"
                     /protein_id="HNE01486.1"
                     /translation="MNKFTEAYRKARDVLENQVFESKWQEFLFADCQARTLFGADGLA
                     VARADDPERVRKRLRDLCKSGNRKIGAVIVEAAQNPVSSGTLAERAATLKMLRHLYHV
                     VKKGGQNVWVYSPPKAYEKWVFDELTGDAKALEAKLNHEKKIFSSTEMRWMASALGVA
                     LKISEDTKAKLGGATGKKDDTDAVIKRWFLDEDSGDAQLTEARTKLLDGFKKIAVACA
                     SDKLVFADYADWIKTRSKYFGAAFRGGEGGGFPVIYLEGAFTRLTGNTGKMWLCAETI
                     IHEFSHHEVSTRDHRYDSSGLKPAKATLPYAKAIDNADSWGYFALDLAGYLSKSDRLN
                     VLK"
     gene            complement(2870..>3380)
                     /locus_tag="PLQ18_07255"
     CDS             complement(2870..>3380)
                     /locus_tag="PLQ18_07255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="lipase family protein"
                     /protein_id="HNE01487.1"
                     /translation="ASGNHGLVSVAAHSMKDSYIPGVGEDNWIGLERRCRDDLNDVRV
                     QGWLDQIASGGGHVLPLGARTLQMIGRALAWLLKKTSALLVGTFGTLLTAGMTVLDQL
                     AWLLAKSAELSIELTGYIAALIKAIFLFLGRTAIVGTVMTFGFVRWVLGLLFGTLTGL
                     ARSALSVLG"
BASE COUNT          505 a         1151 c         1158 g          566 t
ORIGIN      
        1 cacgtagtcg tcggcggcgc gcgccggcac gtcctcgccc gagcacgcgg cgagcatgtg
       61 gcgcggcacc ggattggccg catcgaaggt cagccactgg cggaacacgt cggcggcctc
      121 ggcggtgtgg ccggtgatgt acagcatgta gccgtactga tagtgcgcgc gggcgtgggt
      181 cggatcgatc tcgatgcagc ggcggaaggc atcgagcacg ctcgcgagcg tcgaggtgtg
      241 gcgcagcatc aggccgagct ggtaatgcgc ctcggcgaag tccggcgcca gcgacgcgag
      301 cttcgcgtag gcctcggccg aatcggcgta caggccgcgc gcgcgcagcg tcaggccgag
      361 gttgttccag gcgccggcgt gtccggcgtc gcattcgagc gcggcacggt aagcggtttc
      421 ggcctcgtcg aaacgcccgg cgcgcagatg cacgttgccg aggttattcc aggcgtcggt
      481 gtagcgcggc acggcctgca gcgcccggcc gatcaggtcg agcgcctcgt catcgcgccc
      541 ctgctggtgg cgcagcaggc cgaggtagtg cagcgcatcc gggtggccgg catcggcggc
      601 cagcagttcg agatagatcg gttcggcggc gaggccctgc ccgcgccggt gcagttcgat
      661 ggcttcggac agacggtctg gggcggccgg ggcggcgaga tcggtcatcg gaactccgga
      721 agcgggacgg cggcggacgg tgcggggttc agcgtatacc gccggccgcc gccgcgacga
      781 ccggccggcc gatgcgcgcc gcaggaaccc ggcgccggct tcggtccggg attcagcgcc
      841 ggttttggac tccctgctcc tgcacggccc ggctgcccag gtccagcgtg tagcgccgtc
      901 cgcgctcatc ggtgatttcc agggtccggc cgccgcgccc ggcgcgcagc gaggcgatga
      961 acacgtcctg cacatcggac tccagcttcg ggtcgtaggc agtcgcatag atgcgctggc
     1021 gccagagttc gcggccgctg cgggcgtcgc gggcgacgag gtagccaccg gcctgatcgt
     1081 cgccgaaatc gcgcgtgtcg ggcggtgcct cgtagcgcac cccgtgcagc gtcagcggtg
     1141 caacggccgg cggcgccacg cgcttgccgg ccggcatttc cccgcccgca ccggcgaccg
     1201 tgcccgctgc cagcagtaca gccgcgagca gcgcgcgcac cggcttcact tcgccgtcac
     1261 cgtgcgcgtg gccggatcga cgacgaagcg ccggccgcgc tcgtcgacga ttttcagcgt
     1321 gccgcgcggc ccggtcttca ggctctcgat gaacacgtcc tgcacgtcct cttcgagatc
     1381 ggggtcatag tcgatcacgt agacctgcag cagccacagc gacgcaccgc tcgccgcatc
     1441 gaaggcttcc aggtaaccac cgttctgccc gagcccgcgc tcgcgccccc agtgcacgac
     1501 ctcgtagcgc acgttcccga tcgtgaccgg ttccacctcc ggcggcccga cccgtttcgg
     1561 cgcgggcagg ccggcctcca gttcgtcatc gttcatggct gttctcccga aaagcggacc
     1621 gcatcacttc agcacgttga gacggtcgga cttcgacagg taaccggcca gatcgagggc
     1681 gaaatagccc cagctgtcgg cattgtcgat ggccttggca taaggcagcg ttgccttggc
     1741 aggtttcagg ccgctgctgt cgtagcggtg atcgcgggtg ctgacttcgt ggtgcgagaa
     1801 ctcatggatg atggtttccg cacacagcca catcttgccg gtattgccgg tcaggcgcgt
     1861 gaaggcgcct tcgagataga tcaccggaaa accgccgccc tcgccgccgc ggaaggcggc
     1921 accgaaatac ttgctgcggg tcttgatcca gtcggcgtaa tcggcgaaca ccagcttgtc
     1981 ggaggcacag gcgaccgcga tcttcttgaa accgtcgagc agtttggtgc gtgcctcggt
     2041 cagttgcgca tcgccgctgt cctcgtcgag aaaccagcgc tttatcacgg catcggtgtc
     2101 gtccttcttg ccggtggcgc cgccgagctt cgccttggtg tcttcggaaa tcttcagcgc
     2161 caccccgagt gccgaggcca tccagcgcat ttcggtgctg gagaagattt tcttctcgtg
     2221 attgagcttg gcctccagcg ccttggcatc accggtcagt tcgtcgaaca cccatttctc
     2281 gtaggccttc ggcggtgaat acacccagac gttctggccg cccttcttca cgacgtgata
     2341 gaggtgccta agcatcttca gcgtcgctgc gcgctcggcg agcgtgccgg aggaaaccgg
     2401 attctgcgcg gcctcgacga tcaccgcgcc gatcttgcgg tttccggact tgcagagatc
     2461 gcgcaggcgc ttgcgcacgc gctccgggtc gtcggcgcgg gccacggcca gtccgtcggc
     2521 gccgaacagc gtccgcgcct gacagtcggc aaacaggaat tcctgccact tgctttcgaa
     2581 cacctgattt tcgagcacgt ccctggcctt tctgtaggct tccgtgaact tgttcatgct
     2641 cgcgctcctc ccgggagacc tgagttttct ggccggggca gcggattgct gctgccggac
     2701 tccggccgtt cgtgcggcag ccccctgcgg tgactgccgc ggaatgccac gcgaaccacc
     2761 ccggtacgtg gcgcggatgg ccggccgcgt gccctcgggg ccggccggca gggtcgctga
     2821 agcaggtctg caaacccggc cgatgccgca aggccgccgg ccggcccgct cagccgagca
     2881 ccgacagcgc cgagcgggcc agcccggtca gcgtgccgaa cagcagcccc agcacccagc
     2941 gcacgaagcc gaaggtcatg accgtgccga cgatcgcggt gcggccgagg aacaggaaga
     3001 tggccttgat cagggccgcg atgtagccgg tcagctcgat cgacagctcc gcactctttg
     3061 cgagcagcca ggcgagctgg tcgagcacgg tcatgccggc ggtgagcagc gtgccgaagg
     3121 tgccgaccag cagggcgctg gtcttcttca gcagccaggc gagcgcccgg ccgatcatct
     3181 gcagcgtccg ggcgccgagc ggcagcacgt gaccgccgcc cgaggcgatc tggtcgagcc
     3241 agccctgcac gcggacatcg ttgaggtcgt cgcggcagcg ccgctccagc ccgatccagt
     3301 tgtcctcgcc gacgccgggg atgtagctgt ccttcatcga atgcgcggcg acgctgacca
     3361 gaccgtggtt gccgctggcg
//
LOCUS       DAOQJH010000276         3368 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_18218_length_3368_cov_3.520374, whole genome shotgun sequence.
ACCESSION   DAOQJH010000276 DAOQJH010000000
VERSION     DAOQJH010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3368)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3368)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3368
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18218_length_3368_cov_3.520374"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1178
                     /gene="pmbA"
                     /locus_tag="PLQ18_07260"
     CDS             <1..1178
                     /gene="pmbA"
                     /locus_tag="PLQ18_07260"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HNE01488.1"
                     /translation="LGVTVYFGRRKGSASTSDWREPALLETVRAACDIARYTAEDPWS
                     GLADPARLARAPLPELDLHHPWALSAEQAIDIARDCEAAARGFDARISNSDGASVGRH
                     ESLVVYGNSNGFLGGYPSTRHSLSCSVIAADPQADGRMERDYWYSSDRDASRLEAPDA
                     VGRRAAERVLRRLGARRIGTRTAPVLFVPELARGLVGHFLGAIRGGALYRKSSFLLDR
                     AGTAVFPDFMQIHERPHLPRALGSAAFDGEGVATQDRDLVCDGVLQGYVLDSYSARRL
                     GLETTGNAGGVHNVIVGSSAPAVGYDELLAAMGTGLVVTELMGHGVNAVTGDYSRGAA
                     GFWVENGEPVHPVEEITIAGNLKTMFETLQMIGSDIDLRGNVRCGSMLLAPMTIAGA"
     gene            1394..2824
                     /locus_tag="PLQ18_07265"
     CDS             1394..2824
                     /locus_tag="PLQ18_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012479071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HNE01489.1"
                     /translation="MESFSLTPIPLCVDLDGTLIRSDLLVESFFLLLKHRPLCLFLVP
                     LWLWRGKAVLKYEIARRVQPDYALLPYDTAFLDWLRAEHARGRPLWLCTASHRLLAEP
                     LAEHLGLFERVLASEDGLNLSGARKRERLVAALGERGYDYAGNAAIDLEVWRHARHAI
                     SVDLPARLRPELARVATIEREFAPRPAGLKVYAQALRLHQWVKNLLIFLPLLLSHQID
                     VRAIAASLTAFLAFGLCASSVYLLNDLLDLPSDRRHPRKRQRPFASGALGLVRGALLI
                     PALLALAGLLCLGLPAEFFGMLTLYYALTVAYSVKLKEFVLIDVIALAGLYTSRILAG
                     AAAVSLHNSFWLLAFSVFLFLSLALVKRYAELREMLRRGRERASGRDYSTHDLALLQT
                     LGIASAYNSVLVLALYINSDTSRQLYARPELIWLLCPVLLYWVSRVWLKTHRDEMHDD
                     PVVFAVRDRASRWLLFATGLIVLAAV"
     gene            2821..>3368
                     /locus_tag="PLQ18_07270"
     CDS             2821..>3368
                     /locus_tag="PLQ18_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNE01490.1"
                     /translation="MTATRACAASWGRYPRVVQHARPLTDRDAPLPASDTSWLPYGNG
                     RSYGDSCLNDGGGVLLARGLDRFIGFDPQSGVLACEAGVLLADILALAVPQGWFLPVT
                     PGTRYVTVGGALANDVHGKNHHRAGSFGRHLRGFELLRSDGSRRWCSAHEHADWYGAS
                     IGGLGLTGLVTRVELQLRRIANP"
BASE COUNT          424 a         1222 c         1181 g          541 t
ORIGIN      
        1 gcctcggcgt caccgtctat ttcggcagac gcaagggctc ggcgagcacc tcggactggc
       61 gcgagccggc gctgctcgaa accgtgcgcg ccgcctgcga catcgcccgc tacacggccg
      121 aggacccgtg gtccggcctc gccgatccgg cccggctggc gcgcgcgccg ctgccggaac
      181 tcgacctgca tcacccgtgg gcgctcagcg ccgagcaggc catcgacatc gcccgcgact
      241 gcgaggcggc ggcacgcggc ttcgatgcgc gcatcagcaa cagcgatggc gcctcggtcg
      301 gccggcacga gtcgctggtc gtctacggca actcgaacgg cttcctcggc ggctatccgt
      361 cgacacgcca ttcgctgtcg tgctcggtga tcgccgccga cccgcaggcc gacggccgca
      421 tggagcgcga ttactggtac agcagcgacc gcgacgccag ccgcctcgaa gccccggatg
      481 cggtcggccg gcgcgcggcc gagcgcgtgc tgcgccgcct cggcgcccgg cgcatcggca
      541 cgcgcacggc gccggtgctg ttcgtgccgg agctggcgcg cggcctggtc gggcacttcc
      601 tcggcgcgat ccgcggcggc gcgctgtacc gcaagtcgag cttcctgctc gatcgcgccg
      661 gcaccgcggt gtttccggac ttcatgcaga tccacgagcg cccgcacctg ccgcgcgccc
      721 tcggcagcgc cgccttcgac ggcgagggcg tcgccacgca ggatcgcgat ctggtctgcg
      781 acggcgtgct gcagggctac gtgctcgatt cgtattcggc ccgccggctc ggcctcgaaa
      841 ccaccggcaa cgccggtggc gtgcacaacg tcatcgtcgg gtcgagtgcg ccggccgtcg
      901 gatacgacga actgctcgcg gcgatgggca ccggcctggt cgtcaccgaa ctgatgggcc
      961 acggcgtcaa cgccgtcacc ggcgactact cgcgcggggc ggccggcttc tgggtcgaga
     1021 acggcgagcc ggtgcacccg gtcgaggaaa tcaccatcgc cggcaacctg aagacgatgt
     1081 tcgagacgct gcagatgatc ggcagcgaca tcgacctgcg cggcaacgtg cgctgcggct
     1141 cgatgctgct cgcgccgatg acgatcgcgg gggcgtgagc ggcgcccgat ccggcagggc
     1201 accggcgggc gggcgctgat gcgctgcaac aaatgtcacg atccgtcgat tgccgctcgt
     1261 ggcggcgcgc ggctactctg cgctccgccc tgatcgtcgc cggcgcgtcg ttgccgggtg
     1321 atccgccacc gttcccgtcc gcccccgcga ccggcgcctg ccgcagccgt ccggtacggc
     1381 tgcggctgca ccgatggagt cgttctcctt gacgccaatc ccgctgtgcg tggatctgga
     1441 cggcacgctg atccgttccg atttgctggt cgagtcgttc ttcctgctgc tgaagcaccg
     1501 gccgctgtgc ctgttcctgg tgccgctgtg gctctggcgc ggcaaggcgg tgctgaagta
     1561 cgagatcgcc cggcgcgtgc agcccgatta cgccctgctg ccctatgaca ccgccttcct
     1621 cgactggctg cgcgccgagc atgcgcgcgg ccggccgctg tggctgtgca cggcctcgca
     1681 ccggctgctg gccgagccgc tggccgaaca cctcgggctg ttcgagcgcg tgctggcgag
     1741 tgaggacggg ctcaatcttt ccggcgcacg caagcgcgag cgtctggtcg cggcgctcgg
     1801 cgagcgcggg tacgactacg ccggcaatgc cgcgatcgac ctcgaagtct ggcgccacgc
     1861 gcggcacgcg atcagcgtcg acctgccggc gcggctgcgc ccggagctgg cgcgggtggc
     1921 gacgatcgag cgcgagttcg cgccgcgccc ggcgggcctg aaggtctacg cgcaggcgct
     1981 gcgcctgcac cagtgggtga agaacctgct gatcttcctg ccgctgctgc tcagtcacca
     2041 gatcgatgtc cgcgccatcg ccgcgagcct gaccgccttc ctggccttcg gcctctgtgc
     2101 atcgagcgtc tatctgctca acgacctgct cgacctgccc tccgaccgcc gtcatccgcg
     2161 caagcgtcag cggccgttcg cgagtggcgc gctcgggctg gtgcgcggcg cgctgctgat
     2221 tccggcgctg ctggcgctgg cgggcctgct gtgcctcggc ctgccggcgg agttcttcgg
     2281 catgctgacg ctgtattacg cgctgacggt cgcgtactcg gtgaagctca aggagttcgt
     2341 gctgatcgac gtgatcgcgc tcgccgggct gtataccagc cgcatcctcg ccggcgccgc
     2401 ggcggtcagc ctgcacaact ccttctggct gctggcgttc tcggtgttcc tgttcctgag
     2461 tctcgcgctg gtcaagcgct atgcggagct gcgcgagatg ctgcgccgcg gccgcgagcg
     2521 tgccagcggg cgcgactaca gcacgcacga cctggcgctg ctgcagacgc tcggcatcgc
     2581 ctcggcctac aacagcgtgc tggtgctggc gctgtacatc aacagcgaca ccagccgcca
     2641 gctctacgcc cggccggagc tgatctggct gctgtgtccg gtgctgctgt actgggtcag
     2701 ccgcgtctgg ctgaagacgc atcgcgacga gatgcacgac gacccggtcg tgttcgcggt
     2761 gcgcgaccgc gccagccgct ggctgctgtt cgcaaccggg ctgatcgtgc tggcggcggt
     2821 atgacggcga cacgggcctg cgccgcctcc tggggccggt atccgcgcgt cgtgcagcat
     2881 gcccggccgc tcaccgaccg cgatgcgccg ctgccggcga gcgacacgag ctggctgccc
     2941 tacggcaacg gccgcagcta cggtgattcc tgcctcaacg acggcggcgg cgtgctgctc
     3001 gcacgcgggc tggaccgctt catcggcttc gatccgcaga gcggcgtgct cgcatgcgag
     3061 gccggcgtgc tgcttgccga tatcctcgcg ctggccgtgc cgcagggctg gttcctgccg
     3121 gtgacgccgg gcacgcgcta cgtgaccgtc ggcggcgcgc tcgccaacga cgtgcacggc
     3181 aagaatcacc accgcgccgg cagcttcggc cggcacctgc gtggcttcga gctgctgcgc
     3241 tcggacggca gccgccgctg gtgcagcgcg cacgaacacg ccgactggta cggcgcgagc
     3301 atcggcggcc tcggcctgac cgggctggtg acgcgcgtcg agctgcagct gcggcgcatc
     3361 gccaatcc
//
LOCUS       DAOQJH010000277         3358 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_18308_length_3358_cov_5.538299, whole genome shotgun sequence.
ACCESSION   DAOQJH010000277 DAOQJH010000000
VERSION     DAOQJH010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3358
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18308_length_3358_cov_5.538299"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1017
                     /locus_tag="PLQ18_07275"
     CDS             <1..1017
                     /locus_tag="PLQ18_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase component 1"
                     /protein_id="HNE01491.1"
                     /translation="KGISVQSECPIGLIGDDIEAVSKKKSKEYEGKTIVPVRCEGFRG
                     VSQSLGHHIANDAIRDWVFDKSDGTRHQFEPSPYDVAIIGDYNIGGDAWSSRILLEEM
                     GLRVIAQWSGDGTLAELEKTPKAKLNLIHCYRSMNYISRHMEEKYNIPWLEYNFFGPT
                     KIEESLRAIAAHFDDTIKANAERVIAKYKALTDAVIAKYRPRLEGKKVMLFVGGLRPR
                     HVIGAYEDLGMEVVGTGYEFAHNDDYQRTTHYVKDGTLIYDDVTGFEFEKFVEKVQPD
                     LVGSGIKEKYVFQKMGVPFRQMHSWDYSGPYHGYDGFAIFARDMDMAINSPVWGLTKA
                     PWKK"
     gene            1089..2627
                     /gene="nifK"
                     /locus_tag="PLQ18_07280"
     CDS             1089..2627
                     /gene="nifK"
                     /locus_tag="PLQ18_07280"
                     /EC_number="1.18.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015665399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016612 - molybdenum-iron nitrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0016163 - nitrogenase activity [Evidence
                     IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase molybdenum-iron protein subunit
                     beta"
                     /protein_id="HNE01492.1"
                     /translation="MSQNAEKVLDHFKLFREPEYVEMFESKKKNFEDAVPAEELERVR
                     EWAKSWEYREKNFAREALTVNPAKACQPLGAVFAAVGFESTLPFVHGSQGCVAYYRSH
                     FSRHFKEPTSCVSSSMTEDAAVFGGLQNMIDGLANSNAMYKPKMIAVSTTCMAEVIGD
                     DLNAFIKTAKEKGSVPMDMDVPYAHTPAFVGSHVTGYDNSMKGVLTHFWDNAERTPNE
                     SINLINGFDGYVVGNIRELKRVLGLMGVDYTLIGDQSDVWDTPTDGEFRMYDGGTKIE
                     DVKAAVNAKATLSMQEYCTEKTLAYCADKGQQTVALNHPVGIAGTDRLLMALSELTGK
                     EIPAELLKERGRLVDAIADSQAHIHGKSFAIYGDPDLCYGLTEFLLELGAEPKHVLST
                     NGNKAWAEKMQALFNSSPFGKGCVAYAGKDLWHMRSLLATEPVDFLIGNTYGKYLERD
                     IGTPLIRIGFPVFDRHHHHRSPIWGYQGGMNLLVKLLDKIFDEMDKDTNIPSKTDYSF
                     DIIR"
     gene            2841..3188
                     /locus_tag="PLQ18_07285"
     CDS             2841..3188
                     /locus_tag="PLQ18_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HNE01493.1"
                     /translation="MANVTFSSPSMKKDVTVYATAGDTQTLLAVAKQHKIPVHFECEN
                     GECGSCVVQVTVLTANAPYAIALTEKEKAVLKLAGKITPEQIDNAEVNDVPPPYRLAC
                     QFIVRNEDILVKF"
BASE COUNT          671 a         1075 c         1052 g          560 t
ORIGIN      
        1 aagggcatct cggtgcagtc cgagtgcccg atcggcctca tcggtgacga catcgaggcc
       61 gtctcgaaga agaagtcgaa ggaatacgaa ggcaagacga tcgtcccggt gcgttgcgag
      121 ggcttccgcg gcgtctcgca gtcgctcggc caccacatcg cgaacgacgc gatccgtgac
      181 tgggtgttcg acaagtccga cggcacgcgt catcagttcg agccctcgcc ctacgacgtc
      241 gcgatcatcg gcgactacaa catcggcggc gacgcctggt cctcgcgcat cctgctcgaa
      301 gagatgggcc tgcgcgtgat cgcccagtgg tccggtgacg gcacgctggc cgagctcgag
      361 aagaccccga aggcgaagct gaacctcatc cactgctacc gctcgatgaa ttacatctcg
      421 cggcacatgg aagagaagta caacattccc tggctcgaat acaacttctt cggcccgacc
      481 aagatcgagg aatcgctgcg tgccatcgcc gcgcatttcg acgacacgat caaggccaat
      541 gccgagcgcg tgatcgccaa gtacaaggcc ctgacggatg ccgtcatcgc caagtaccgt
      601 ccgcgcctcg aaggcaagaa ggtcatgctg ttcgtcggcg gcctgcgccc gcgtcacgtg
      661 atcggtgcct acgaagatct gggcatggaa gtggtcggca cgggttatga gttcgcccac
      721 aacgacgact accagcgcac cacgcattac gtgaaggacg gcacgctgat ttacgacgac
      781 gtcaccggct tcgagttcga gaagttcgtc gagaaggtcc agcccgacct ggtcggttcc
      841 ggcatcaagg aaaagtacgt gttccagaag atgggcgtgc cgttccgtca gatgcactcc
      901 tgggactact ccggtccgta tcacggctac gacggcttcg ccatcttcgc ccgtgacatg
      961 gacatggcga tcaactcgcc ggtctggggc ctgaccaagg cgccgtggaa gaagtgatta
     1021 gcgtgcgggg cggcaagccg acatacgccc cgcctttacc cgaattcatg tgattttgga
     1081 gacaggccat gagccagaac gctgaaaagg tgctggacca cttcaagctg ttccgcgagc
     1141 cggaatatgt ggagatgttc gagtccaaga agaagaactt cgaggacgcg gtgccggcgg
     1201 aagaacttga gcgcgtccgc gagtgggcga agagctggga ataccgcgaa aagaatttcg
     1261 cccgcgaagc cctgacggtg aatccggcca aggcctgtca gccgctcggt gcggtgttcg
     1321 ccgcggtcgg cttcgagagc acgctgccgt tcgtgcacgg ttcgcagggt tgcgttgcgt
     1381 actaccgctc gcacttcagc cgtcacttca aggagccgac cagctgcgtc tcctcgtcga
     1441 tgaccgaaga cgccgcggtg ttcggcggcc tgcagaacat gatcgacggc ctcgcgaaca
     1501 gcaacgcgat gtacaagccg aagatgatcg ccgtgtcgac cacctgcatg gcggaagtca
     1561 tcggtgacga cctcaacgcc ttcatcaaga ccgccaagga aaagggctcg gtgccgatgg
     1621 acatggatgt gccgtatgcg cacaccccgg ccttcgtcgg cagccacgtg accggctacg
     1681 acaactcgat gaagggcgtg ctgacgcact tctgggataa cgccgagcgc accccgaacg
     1741 agtcgatcaa cctcatcaac ggtttcgacg gctacgtcgt cggcaacatc cgcgagctca
     1801 agcgcgtgct cggcctgatg ggcgtggact acacgctgat cggtgaccag agcgacgtgt
     1861 gggatacgcc gaccgatggc gaattccgca tgtacgacgg cggcaccaag atcgaggacg
     1921 tgaaggccgc ggtcaacgcc aaggccacgc tgtcgatgca ggaatactgc accgagaaga
     1981 cgctcgccta ctgcgccgac aagggccagc agacggtcgc gctgaatcac ccggtcggca
     2041 tcgccggcac cgatcgcctg ctgatggcgc tctccgagct gaccggcaag gagatcccgg
     2101 cggagctgct gaaggagcgc ggccgtctgg tcgacgcgat cgccgactcg caggcgcaca
     2161 tccacggcaa gagcttcgcg atctacggcg atccggacct ctgctacggc ctgaccgagt
     2221 tcctgcttga actcggcgcc gagccgaagc acgtgctctc gaccaacggc aacaaggcct
     2281 gggccgagaa gatgcaggcg ctgttcaaca gctcgccgtt cggcaagggc tgcgtcgcct
     2341 acgccggcaa ggacctctgg cacatgcgct cgctgctggc gacggagccg gtcgacttcc
     2401 tgatcggcaa cacctacggc aagtacctgg agcgtgacat cggcaccccg ctgatccgca
     2461 tcggcttccc ggtgttcgac cgtcaccacc accaccgctc gccgatctgg ggctatcagg
     2521 gtggcatgaa cctgctcgtc aagctgctcg acaagatctt cgacgagatg gacaaggaca
     2581 ccaacatccc gtccaagacc gattacagct tcgacatcat ccgctgaggc tgcccggacg
     2641 caccgcgcgt gcgtgaaacc cgccccgagc ccggggcggg cgggggaacc ggaggcgccg
     2701 gctccgaccc ccactcccgg cggccccctg agtgcgcttt ctgcggagag ccggggccgc
     2761 ctctctattt cgggcgggtg ccgttgctga aggccggcga ccgtgaaggc ttccgtcgtt
     2821 acatctggag aatcaggatc atggccaacg ttacgttcag ttcgccgagc atgaagaagg
     2881 atgtcaccgt ctacgcgacg gccggcgaca cgcagacgct gctggcggtc gccaagcagc
     2941 acaagatccc ggtgcacttc gagtgcgaga acggcgagtg cggttcctgc gtcgtgcagg
     3001 tcaccgtgct gaccgccaac gccccgtacg cgatcgcgct gacggagaag gaaaaggcgg
     3061 tcctgaagct cgccggcaag atcacgccgg agcagatcga caacgccgag gtcaacgacg
     3121 tgccgccgcc ctaccgcctg gcctgccagt tcatcgtgcg caacgaggac atcctcgtga
     3181 agttctgatt gcggccggca cccggtgacg ggtgctgcca gggaagcgcc tcgacccgcc
     3241 gcctgcacgg cgggtcggtt tttttcggtt ccgtgaccgc caggcgacgg tcgtccgtgc
     3301 gggcagcgcc aaccttaccg ttctgtcatc agcgtttcat gctgcgttgc cacactgc
//
LOCUS       DAOQJH010000278         3358 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_18309_length_3358_cov_4.293672, whole genome shotgun sequence.
ACCESSION   DAOQJH010000278 DAOQJH010000000
VERSION     DAOQJH010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3358
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18309_length_3358_cov_4.293672"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..158
                     /locus_tag="PLQ18_07290"
     CDS             <1..158
                     /locus_tag="PLQ18_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013698826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aromatic amino acid aminotransferase"
                     /protein_id="HNE01494.1"
                     /translation="MFSYSGLSAAQVERLKTEHGIYAVGTGRICVAALNTTNLPTVAA
                     AIAEVLR"
     gene            251..326
                     /locus_tag="PLQ18_07295"
     tRNA            251..326
                     /locus_tag="PLQ18_07295"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:284..286,aa:Asn,seq:gtt)
     gene            415..490
                     /locus_tag="PLQ18_07300"
     tRNA            415..490
                     /locus_tag="PLQ18_07300"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:448..450,aa:Asn,seq:gtt)
     gene            699..854
                     /locus_tag="PLQ18_07305"
     CDS             699..854
                     /locus_tag="PLQ18_07305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE01495.1"
                     /translation="MSMLAIIGLLLPSATVAVFAEWRARRIGAGTARRKAAEPWQRSG
                     SLFFSHD"
     gene            complement(873..2276)
                     /gene="asnS"
                     /locus_tag="PLQ18_07310"
     CDS             complement(873..2276)
                     /gene="asnS"
                     /locus_tag="PLQ18_07310"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008213235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HNE01496.1"
                     /translation="MTDLPTIATLLGGTVAVGTPVTVQGWVRTRRDSKAGLSFVAVHD
                     GSCFDAIQCVVPDTLANYRSEVLHLGSGCSVRVTGTLVASGGKGQTVEVQAAALEVIG
                     LVEDPETYPVQPKPHTFEYLRTVAHLRPRTNTFGAVARVRHCLAQAIHRFFSAHGYCW
                     VHTPIITASDCEGAGELFRVSTLDFANLPRTAEGRIDYAQDFFGREAFLTVSGQLNVE
                     AYCLALSRVYTFGPTFRAENSNTSRHLAEFWMVEPEIAFADLAADADLAEALLKSVFR
                     AVLDERGDDLAFFDDRILKGCIARLEALVEQPFARIDYSEAVRVLEGCGETFQFPVAW
                     GADLQSEHERFLAERHVGGPVVVMNYPKAIKSFYMRLNDDGRTVAAMDVLAPGIGEII
                     GGSQREERLDVLDARMRECGLDPAGYGWYRDLRRYGTVPHAGFGLGFERLVSYVTGMA
                     NVRDVIPFPRTPGSAEF"
     gene            2373..3152
                     /locus_tag="PLQ18_07315"
     CDS             2373..3152
                     /locus_tag="PLQ18_07315"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HNE01497.1"
                     /translation="MQLIAVQTDIRWEDKPANHEHVRELLAAAAPPPGALAVLPEMCM
                     TGFSMKVAKIDEGEARPGEQFLSALAQEFGIFICAGLVTRADDGRGLNQSVTWAPDGS
                     ELARYSKCHPFSFAHEDRHYAKGDGPVLYEWAGLRVAPTICYDLRFPELYRIAAGRGA
                     QLYTVIANWPQRREAHWRALLVARAIENQAWVIGVNRCGDDPWLHYGGASLIIDPRGE
                     IIADAGSGPGTIRAEVHLDALLDYRASFPALADMRGEFVPV"
BASE COUNT          530 a         1169 c         1135 g          524 t
ORIGIN      
        1 gcatgttctc gtactcgggc ctgagcgccg cccaggtcga gcgcctgaag accgagcacg
       61 gcatctacgc ggtcggcacc gggcgcatct gcgtggccgc actgaacacc accaacctgc
      121 cgacggtcgc cgccgcgatc gcggaagtcc tgcgctgagc gtcgcgcagc ggccgcccgg
      181 atacgggcgg ccgctgcatc gcggttgacg cgcgtggcgg ccatctatag gatgcgcggc
      241 tcgcaacggt tccccgatag ctcagtcggt agagcaagtg actgttaatc actgggtcgg
      301 cggttcgagt ccgtctcggg gagccaatta ccgaatttac ctgtaccgcc gtgctgcatt
      361 gcaatgtctg cacgtcggca aagtgattca ccggtttacg acaatttaat tcattccccg
      421 atagctcagt cggtagagca agtgactgtt aatcactggg tcggcggttc gagtccgtct
      481 cggggagcca atttggaggg ttgcgatcat cgcaaccctc ttttactttc tccgccagcc
      541 atcgctgccg aataagcaga accgcccgcg ccctgcccgg cccgggaaac aaggcatcac
      601 agcgatggcc cgccacgttc gttacgctgt cccgcatgct ttctgatcga ttaaccgatc
      661 cgcacggaca gcggacattg gagggggagg tgcctgtcat gtctatgctc gccatcatcg
      721 gacttctgct gcccagcgcc acggttgccg tgtttgccga atggcgcgcc cgacgcatcg
      781 gtgccggcac cgcccggcgc aaggccgccg agccctggca gcgcagcggc agcctgttct
      841 tctcgcacga ctgactgcgc cccgcccggg gttcagaact cggcgctgcc gggcgtgcgc
      901 ggaaacggga tcacgtcgcg cacgttcgcc atgccggtca cgtagctgac caggcgctcg
      961 aaccccagtc cgaagcccgc atgcggcacc gtgccgtagc ggcgcagatc ccggtaccag
     1021 ccgtagccgg ccggatccag tccgcattcg cgcatgcgcg catcgagcac gtcgagacgc
     1081 tcctcgcgct ggctgccgcc gatgatctcg ccgatgccgg gcgcgagcac gtccatcgcc
     1141 gcgaccgtgc gcccgtcgtc gttcaggcgc atgtagaagc tcttgatcgc cttcgggtag
     1201 ttcatgacca cgaccggccc gccgacatgg cgctcggcca gaaagcgctc gtgctcggac
     1261 tgcaggtccg ctccccaggc gaccgggaac tggaaggtct cgccgcagcc ctccagcacg
     1321 cgcaccgcct cgctgtagtc gatgcgcgcg aacggctgtt cgaccagcgc ttcgaggcgc
     1381 gcgatgcagc ccttcaggat gcggtcgtcg aagaacgcga gatcatcgcc gcgctcgtcg
     1441 agcacggcgc ggaacacgga cttcagcagc gcctcggcca gatcggcgtc ggcggcgaga
     1501 tcggcaaagg cgatctccgg ctcgaccatc cagaactcgg cgaggtggcg cgaggtgttg
     1561 gagttctcgg cgcggaaggt cgggccgaag gtatatacgc gcgacagcgc caggcagtag
     1621 gcctcgacgt tcagctgccc ggacacggtc aggaaggcct cgcgcccgaa gaagtcctgc
     1681 gcgtagtcga tgcggccctc ggccgtgcgc ggcaggttgg cgaaatcgag cgtgctgacc
     1741 cggaacagct cgccggcgcc ctcgcagtcg ctggcggtga tgatcggcgt gtggacccag
     1801 cagtagccgt gcgcgctgaa gaagcggtgg atcgcctgcg ccaggcaatg gcgcacgcgc
     1861 gcgaccgcgc cgaaggtgtt ggtgcgcgga cgcaggtgcg cgaccgtgcg caggtattcg
     1921 aaggtgtgcg gcttcggctg caccggatag gtttcggggt cctcgaccag cccgatgact
     1981 tcgagcgccg cggcctgcac ctcgaccgtc tggcccttgc cgcccgaggc caccagcgtg
     2041 ccggtgacgc gcaccgagca gccgctgccg aggtgcagca cctcgctgcg gtagttcgcg
     2101 agcgtatccg gcaccacgca ctggatcgcg tcgaagcagg agccgtcatg caccgcgacg
     2161 aacgacagcc ccgccttcga atcgcggcgt gtgcgcaccc agccctgcac ggtgaccggc
     2221 gtgccgacgg cgacggtacc gccgagcagc gtggcaatcg tgggcagatc ggtcatcgtc
     2281 ggcactcccg gcaagtcagc agcgcggatc gcggcgccgg caatggtggc atattgccgc
     2341 cctgaccgtg acctcgcgca gggtgccccg ccatgcagct catcgccgtg cagaccgaca
     2401 tccgctggga agacaagccc gccaatcacg aacacgtgcg ggagctgctc gccgccgccg
     2461 ccccgccgcc gggcgcgctg gccgtgctgc ccgagatgtg catgaccggc ttcagcatga
     2521 aggtcgcgaa gatcgatgag ggcgaggcgc gcccgggcga gcagttcctg agcgcgctgg
     2581 cgcaggaatt cggcatcttc atctgcgccg gcctcgtcac gcgcgccgat gacggccgcg
     2641 gcctgaacca gtcggtgacc tgggcgccgg atggcagcga gctggcgcgc tacagcaagt
     2701 gccatccgtt ctcgttcgcg cacgaggacc gccactacgc caagggcgac ggccccgtgc
     2761 tgtacgaatg ggccgggctg cgggtcgcgc cgaccatctg ctacgacctg cgcttcccgg
     2821 agctgtaccg gatcgccgcc gggcgcggcg cgcagctcta cacggtcatc gccaactggc
     2881 cgcagcggcg cgaggcgcac tggcgggcgc tgctggtggc acgcgcgatc gagaaccagg
     2941 cctgggtaat cggcgtcaac cgctgcggcg acgacccgtg gctgcactac ggcggcgcca
     3001 gcctgatcat tgacccgcgc ggtgagatca tcgccgacgc cggcagcggc cccggcacga
     3061 tccgcgccga ggtccacctc gatgcgctgc tcgactaccg cgcgagtttc ccggcgctgg
     3121 ccgacatgcg cggcgagttc gtgccggtct gatgcctgcc ccgggtccgt aaaaacgacg
     3181 gggcggcgga ccttgcggcc ggccgccccg ccaggggggg actgcgcgtg acgtgcttac
     3241 ttgctggtgc gacggcgacg cgtcttcggc gccgaatcgc cgccctcgcc ctgcgactca
     3301 ccggcatccg ccacggtgtc atcggcatcg tgctcgccgc actgctgctg ccggcgac
//
LOCUS       DAOQJH010000279         3354 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_18341_length_3354_cov_4.572901, whole genome shotgun sequence.
ACCESSION   DAOQJH010000279 DAOQJH010000000
VERSION     DAOQJH010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3354)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3354)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3354
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18341_length_3354_cov_4.572901"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..199)
                     /locus_tag="PLQ18_07320"
     CDS             complement(<1..199)
                     /locus_tag="PLQ18_07320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE01498.1"
                     /translation="MTQKKNLAVLAAITLSLAVAGCAQPGAGGGGSSSGSGMSSTATG
                     AVAGAALGALAGGLSGKHDKAK"
     gene            complement(655..1332)
                     /locus_tag="PLQ18_07325"
     CDS             complement(655..1332)
                     /locus_tag="PLQ18_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006463578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase"
                     /protein_id="HNE01499.1"
                     /translation="MSELLPTVEIEPAVPATATVIWLHGLGADGHDFEPVVPELGLPP
                     GHTIRFVFPHAPRRPVTVNGGFVMRAWYDILGFDGPVSEDAAGIRASAAQITALIARE
                     NARGIPDARIVLAGFSQGAAMALHTGLRHRERLAGLLALSGYLPLAATLAAERSPANA
                     ATPVFLAHGEIDAVVRFPRGTATRDRLVQLGWQPEWHDYPIGHEVCWEELRDIGAFLN
                     RVLPARP"
     gene            1430..1576
                     /locus_tag="PLQ18_07330"
     CDS             1430..1576
                     /locus_tag="PLQ18_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008366727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HNE01500.1"
                     /translation="MPRTVSARSVLLVLAALLAFTVAGCGQKGDLYLPGGKPQKVDDR
                     NRSK"
     gene            1620..2870
                     /gene="lysA"
                     /locus_tag="PLQ18_07335"
     CDS             1620..2870
                     /gene="lysA"
                     /locus_tag="PLQ18_07335"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007192164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HNE01501.1"
                     /translation="MDHFEYRQGQLYAEDVPVADLARRFGTPCYVYSRATLTRHFRVF
                     DDAFGSHPHLVCYAVKANGNLGVLNVLARLGSGFDIVSVGELERVLAAGGDPGKVVFS
                     GVGKREDEIARALEVGIRCFNVESEAELERLQQVAAAHGVQAPVSLRVNPDVDAGTHP
                     YISTGLKENKFGIAIADAPAVYARAASRANLRVTGVDCHIGSQLTTLAPFTDTLDRLL
                     LLVERLAADGIGIDHIDLGGGIGIRYRDEQPPHPSQHIAAVLERLAGLPYAVYVEPGR
                     AIVGNAGLLLTRVEFLKCSEHKNFAIVDAAMNDLIRPSLYSAWMEIIPADATLERAQQ
                     RYDVVGPICETGDFLGKDRELAVAEGDLLAVRSAGAYGFTMSSNYNARPRAAEVLVDG
                     SEVHLVRARESLASLYAGESVLPA"
     gene            2867..3295
                     /locus_tag="PLQ18_07340"
     CDS             2867..3295
                     /locus_tag="PLQ18_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HNE01502.1"
                     /translation="MSFTNPGPDAIRARLAQVRSIAVVGQSDSPGRPSYGVALGLQGL
                     GYRIIPVNPRLNHALGLPAYASLDELPAVPDLVDVFRASEHVAGIVDDCIRLGVPALW
                     LQDGVIDAAAARRAQAAGIFVVMDRCLWRDCVQLGVRAAR"
BASE COUNT          496 a         1192 c         1156 g          510 t
ORIGIN      
        1 gcttggcctt gtcatgcttg ccgctgaggc cgccggccag ggcgccgagt gcggcaccgg
       61 cgacggcacc ggttgcggtg ctgctcatgc cgctgccgct gctgctgccg ccaccgccgg
      121 caccgggctg ggcgcagccg gccacggcga gcgagagcgt gatggcggcc agcacggcca
      181 ggttcttctt ctgggtcatg gagtcttcct ctgcttcccg gggaaaccgg cgcccgttgc
      241 ggtgggccgg tgcgaggacg tcgcgagggt caggacaggc aaggaccgcg caggtgcgac
      301 gcccggatcg gggcaaaaga atagccggtg tcaccagagg gacaccggct cgaaggcggg
      361 gtgggtcttg tgacggggcc ggacgacctc ctgccgaccg ggaacctcgt ctgttatggc
      421 tgctgatgcc agcgcaaaag catcttgcat gccaatcttt tcattgcatc agaacaatta
      481 cttgccgggt gcccgcggcg cagccgccgg ttttccggcg ccggcgtccg caaaacggcg
      541 tcaaaaatgc aaggtcgatg gcatttcggg aatgcagcgg cgggcggggt gctgcaatcc
      601 gcagcccgcc ggcgaactgc agcgcaggat gccagcgggc tcccgttcac cggctcaggg
      661 ccgggccggc agcacgcggt tcaggaaggc gccgatgtcg cgcagttcct cccagcagac
      721 ctcgtggccg atcggatagt cgtgccattc gggctgccag ccgagctgca ccagccggtc
      781 gcgcgtcgcc gtgccgcgcg ggaagcgcac gacggcatcg atctcgccgt gcgcgaggaa
      841 taccggcgtc gcggcgttgg ccgggctgcg ctcggcggcg agcgtcgcgg caagcggcag
      901 atagcccgac agcgccagca gcccggccag gcgttcccga tggcgcaggc cggtgtgcag
      961 cgccatcgcc gcgccctgcg agaagccggc gagcacgatg cgcgcatccg ggatgccgcg
     1021 ggcgttctcg cgcgcgatca gcgccgtgat ctgcgcggcc gaggcgcgga tgccggcagc
     1081 atcctcgctg accgggccat cgaagccgag gatgtcgtac caggcgcgca tcacgaaacc
     1141 gccgttgacg gtcaccggcc ggcgcggcgc gtgcgggaac acgaagcgga tcgtgtgtcc
     1201 cggcggcagg ccgagttccg gcaccaccgg ctcgaagtcg tggccgtcgg cgccgagccc
     1261 gtgcagccag atcacggtgg cggtcgccgg tacggcgggt tcgatttcga cggtcggcag
     1321 cagctcgctc atctcgggac acctgtcggt acggaggggg aagggtgcag tgtaagcgcc
     1381 gcggtatact cgccggccca tccgcctgcc gtcgccggaa acctccgcca tgccccgcac
     1441 cgtgtccgcc cgttccgtgc tgctcgtcct cgccgcgctg ctggcgttca ccgtcgccgg
     1501 ctgcggccag aagggtgacc tctacctgcc gggcggcaag ccgcagaagg tcgacgatcg
     1561 caaccgctcg aagtgatccg ccccgccccg tccgatcgcc gctccagaga atccgcgcca
     1621 tggaccattt cgaataccgc cagggtcagc tgtacgccga agacgtgccg gtcgccgacc
     1681 tcgcccggcg cttcggcacg ccgtgctacg tgtactcgcg cgcgacgctg acccggcatt
     1741 tccgcgtctt cgacgacgcc ttcggcagcc atccgcacct ggtctgctac gcggtcaagg
     1801 ccaacggcaa cctcggcgtg ctgaacgtgc tcgcgcgcct cggttcgggc ttcgacatcg
     1861 tctcggtcgg cgagctggag cgcgtgctgg ccgccggcgg tgatccgggc aaggtggtgt
     1921 tctccggcgt cggcaagcgc gaggacgaga tcgcccgcgc gctggaggtc ggcatccgct
     1981 gcttcaacgt cgagagcgag gccgaactcg aacgtctgca gcaggtcgcc gccgcgcacg
     2041 gcgtgcaggc gccggtcagc ctgcgcgtga accccgatgt cgacgccggc acgcatccgt
     2101 acatctccac cggcctcaag gagaacaagt tcggcatcgc cattgccgat gcaccggccg
     2161 tctacgcccg cgccgccagc cgggcgaacc tgcgcgtgac cggggtcgac tgccacatcg
     2221 gctcgcagct gaccacgctc gcgccgttca ccgacacgct cgaccggctg ctgctgctgg
     2281 tcgagcggct cgctgccgac ggcatcggca tcgatcacat cgacctcggc ggcggcatcg
     2341 gcatccgcta tcgcgacgag cagccgccgc acccgagcca gcacattgcc gcggtgctcg
     2401 aacgcctggc cggcctgccg tacgcggtct acgtcgagcc gggccgtgcg atcgtcggca
     2461 acgccgggct gctgctgacg cgcgtcgagt tcctgaagtg ttcggaacac aagaacttcg
     2521 cgatcgtcga tgcggcgatg aacgacctga tccggccgtc gctgtactcg gcgtggatgg
     2581 aaatcatccc ggccgacgcg acgctcgaac gcgcgcagca gcgctacgac gtggtcggcc
     2641 cgatctgcga gaccggcgac ttcctcggca aggaccgcga actcgccgtg gccgaaggcg
     2701 acctgctggc cgtgcgctcg gccggggcct acggcttcac gatgagcagc aactacaacg
     2761 cccgcccgcg cgccgccgag gtgctggtcg acggcagcga ggtccatctg gtgcgcgcgc
     2821 gcgaatcgct ggcgagcctc tacgccggcg aatcggtgct gccggcatga gcttcaccaa
     2881 ccccggcccc gatgccatcc gcgcccggct tgcgcaggtg cgcagcatcg ccgtcgtcgg
     2941 ccagtcggat tcgccgggcc ggccgagcta cggcgtcgcc ctcggtctgc aggggcttgg
     3001 ctaccggatc atcccggtca acccgcggct gaaccacgcc ctcggcctgc ccgcgtacgc
     3061 ctcgctcgac gaactgccgg ccgtgccgga tctggtcgac gtgttccgcg cctccgaaca
     3121 cgtcgccggc atcgtcgacg actgcatccg cctcggcgtg ccggcgctgt ggctgcagga
     3181 tggcgtcatc gatgccgccg ccgcgcgccg tgcgcaggcg gccggcatct tcgtggtcat
     3241 ggaccgctgc ctgtggcgcg actgcgtgca acttggcgtg cgtgcggccc gctgatcgac
     3301 attagttgca attcaacaac tgtgcttaac agccacacaa caactcctat catc
//
LOCUS       DAOQJH010000280         3345 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_18445_length_3345_cov_4.269301, whole genome shotgun sequence.
ACCESSION   DAOQJH010000280 DAOQJH010000000
VERSION     DAOQJH010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3345
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18445_length_3345_cov_4.269301"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..332)
                     /locus_tag="PLQ18_07345"
     CDS             complement(<1..332)
                     /locus_tag="PLQ18_07345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE01503.1"
                     /translation="MTADPEKTSAFHEAPAAAGATPAGTTLPASAAWRATLDAAVAAA
                     GQAWLAGRRTRVAPFARRHFGWRGAWRLNRRAFGKDLLRAPVNVLWAAPHLLLQLGAR
                     AGRKAGLKR"
     gene            complement(543..1853)
                     /locus_tag="PLQ18_07350"
     CDS             complement(543..1853)
                     /locus_tag="PLQ18_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019341195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNE01504.1"
                     /translation="MQDLHGHALSGASAQALDAFEIALRELRCLSGDPLGHAEAAVAA
                     APEFTMAHALRAWLFLVGTDAGAVPLAREALAQARALPADAREARHLAAIGHWCAGRW
                     RLAARTLEDLSLEHPHDLLALQLGQQLDFFTGDQRLLRDRIARALPAWSPDMPGYHAL
                     LGMYAFGLEENGDFHNAERYGRRAIELEPRDAWAQHAVVHVLEMQGRRDDGIRWMTGQ
                     SGWKQCGLLAVHNWWHLALHYLGLDDVDAALQLLDGPIDGHRPTLALELIDVSALLWR
                     LRLRGVDVGERWVSVAERWSACAGHGWYAFNDLHAAMAFACAGRADLLEQLDATQRAA
                     QTRADDNAGFLLAVGRAATEAVIAHVAGDHRRTVERLRDLRSHARAFGGSDAQRDLLD
                     QTLIHSARASGQPALARGLEAERSLLARQRAGARPAVALQPAVH"
     gene            complement(2011..2958)
                     /locus_tag="PLQ18_07355"
     CDS             complement(2011..2958)
                     /locus_tag="PLQ18_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010999337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HNE01505.1"
                     /translation="MSAIAPSAASCTSSSDRFSGRFSGRSASRRPARWRRQLLGAALA
                     ATAAASAGASPLLFSGTGAAATTAFDAFRGAIGGGSRITWDGVRLDGSDVNPATQVLI
                     PGSTVAIPENRFTAAGALFADPYAVAGDGFAAANPATAGQFPAFTPGNTFVMFDTDGS
                     DFDDRFIEQSFVLPGTTTAAGTRGFGAIFVGVEQAGSSSIEYFGRNALGERISLGQFA
                     VEAGSGPSDTQFLGVLFDAAIVADVVLTVGTHALFGFDGTTLRSFGAQTPGTDLAVTD
                     DFVFARPEALVTVPEPPPLALGFAALGAALTLRRLRPAD"
     gene            complement(3102..>3345)
                     /locus_tag="PLQ18_07360"
     CDS             complement(3102..>3345)
                     /locus_tag="PLQ18_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008216887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE01506.1"
                     /translation="TATWPPYVVKAGENPAGMSAITREDGSRQWSLAGRPLYFFAGDQ
                     KPGDARGDGVGGVWHLARNAAPAASVASSSGSSYGY"
BASE COUNT          479 a         1212 c         1231 g          423 t
ORIGIN      
        1 cgtttcagcc cggccttgcg tccggccctg gccccgagct gcagcagcag gtgcggtgcg
       61 gcccacagca cgttgaccgg cgcgcgcagc agatccttgc cgaaggcacg ccggttcagc
      121 cgccaggcgc cgcgccagcc gaaatgccgg cgcgcgaacg gcgcgacacg ggtgcggcgc
      181 ccggcaagcc aggcctgacc ggccgcggcg acggcggcat cgagcgtcgc gcgccaggca
      241 gcgctggccg gcagcgttgt gccggccgga gtcgcgccgg ccgcagcggg ggcttcgtgg
      301 aaggcagagg tcttttcggg atcggcagtc atccggatat cgacccggcg catggcgctg
      361 cggttcgcag ccctggccgg atgatgccgc agcgcgatgc ccgctgccgg ccgggatcgg
      421 caggcatccc ggtccgcagg gcagcgatca gaacaccatc acccgccatg attccgtgcc
      481 atgccgcccc ctcatccccg gtccttcccc cggtggggga aggatggaaa tcaactgccg
      541 gatcagtgca ctgcaggttg cagcgccacc gcgggtctgg cgccggcgcg ctggcgggcc
      601 agcagcgagc gttcggcttc caggccgcgg gccagcgccg gctggccgct cgcgcgcgcg
      661 gagtggatca gcgtctggtc gagcaggtcg cgctgcgcgt cgctgccgcc gaaggcgcgg
      721 gcgtggctgc gcaggtcgcg cagccgctcg accgtgcgcc ggtgatcgcc ggcgacgtgc
      781 gcgatcacgg cctcggtcgc cgcccgcccg accgccagca ggaagccggc gttgtcgtct
      841 gcgcgcgtct gcgccgcacg ctgcgtggca tcgagctgtt cgagcaggtc cgcccggccg
      901 gcacaggcga aggccatcgc cgcgtgcagg tcgttgaacg cataccagcc gtgcccggcg
      961 caggcgctcc agcgctcggc gaccgacacc cagcgctcgc cgacatcgac gccgcgcagg
     1021 cgcaggcgcc agagcagcgc gctcacgtcg atcagctcca gcgccagcgt cggccggtgc
     1081 ccgtcgatcg ggccgtcgag cagttgcagc gccgcatcga catcatcgag gccgaggtaa
     1141 tgcagggcca gatgccacca gttgtgcacg gcgagcagcc cgcactgctt ccagccggac
     1201 tgaccggtca tccagcggat gccgtcatcg cgccggccct gcatctccag cacgtgcacg
     1261 accgcgtgct gcgcccaggc gtcgcgcggc tccagttcga tcgcccggcg gccgtagcgc
     1321 tcggcgttgt gaaaatcgcc gttctcctcc agcccgaacg catacatgcc gagcagcgcg
     1381 tgatagccgg gcatgtccgg cgaccaggcc ggcagcgccc gggcgatgcg atcccgcagc
     1441 aggcgctggt cgccggtgaa gaagtcgagc tgctgcccga gctgcagcgc cagcaggtcg
     1501 tgcggatgct cgaggctcag gtcttcgagc gtgcgcgccg ccagccgcca gcgtccggca
     1561 caccagtgcc cgatcgccgc cagatgccgt gcctcccggg catcggccgg cagggcccgg
     1621 gcctgcgcca gcgcctcgcg cgccagcggc acggccccgg cgtcggtgcc gaccagaaac
     1681 agccaggcac gcagcgcatg cgccatcgtg aactccggcg cggccgcgac cgccgcctcg
     1741 gcgtggccga gcgggtcacc actcaggcag cgcagttcgc gcagcgcgat ttcgaaggcg
     1801 tcgagggcct gggcactggc gccgctgagc gcgtgaccgt gcagatcctg catcgtcatg
     1861 atgaaaactc cggaagtcgg gatggccggg tccctgcccg gcctgtcgtc aagggggatg
     1921 tgcggcgcgc gcagcgcggc cgctgtcgcg ggagatcgcc gcccgcaggc tgttgctggt
     1981 cgtggccggc gagcggcgga cagcaggctt tcagtcggcc ggccgcagcc ggcgcagcgt
     2041 cagcgcggca ccgagggccg cgaagccgag cgcgagcggc ggcggctccg gcaccgtgac
     2101 cagcgcctcg gggcgcgcga acacgaagtc gtcggtgacg gcgagatcgg tccccggcgt
     2161 ctgcgcgccg aagctgcgca gcgtcgtgcc gtcgaagccg aacagcgcgt gcgtgccgac
     2221 cgtcagcacc acgtcggcaa cgatcgccgc atcgaacagc acgccgagga actgcgtgtc
     2281 ggatggcccg ctgccggcct cgaccgcaaa ctgcccgagg ctgatgcgct cgccgagcgc
     2341 gttgcggccg aagtactcga tgctgctgct gccggcctgc tcgacgccga cgaagatcgc
     2401 cccgaagccg cgcgtaccgg ccgcggtcgt cgtgccgggc agcacgaagc tctgctcgat
     2461 gaagcggtcg tcgaagtcgc tgccgtcggt atcgaacatc acgaaggtgt tgccgggcgt
     2521 gaaggccgga aactggccgg cggttgccgg gttggcggcg gcgaagccat cgccggcgac
     2581 cgcatacgga tcggcgaaca gcgccccggc ggcggtgaag cggttctcgg ggatcgccac
     2641 cgtgctgccg gggatcagca cctgggtcgc cggattcacg tcgctgccat cgaggcgcac
     2701 gccgtcccag gtgatgcggc tgccgccacc gatcgcgccg cggaaggcat cgaaggcggt
     2761 cgtcgccgcg gcaccggtgc cggagaacag cagcggcgac gcaccggccg aggcggccgc
     2821 ggtcgcggcc agcgcagccc ccagcagctg ccgccgccag cgcgccggac gacggctggc
     2881 ggagcggcct gagaaacggc ctgagaaacg gtctgaggag ctggtgcagg aagcagcaga
     2941 aggcgcaatc gcggacatgg gaaacctccc gggtcgggca cgaaggcccg gctcgctgtc
     3001 gactggatgt gttctgtcat cgaatcgctg cgcctgcgcc gcgatccggg gcgcgcaaga
     3061 cccgctcgtg caggccggcg cctcagcgcc tgacagccgg ctcagtagcc gtagctgctg
     3121 ccgctgctgc tcgcgacgct cgccgccggc gcagcattgc gcgcgaggtg ccagacgccg
     3181 ccgacgccgt cgccgcgcgc atcgccgggc ttctggtcgc cggcgaagaa gtacagcggc
     3241 cggccggcca gcgaccactg gcggctgccg tcctcgcggg tgatcgcgct catgccggcc
     3301 gggttttcac cggctttgac cacatacggc ggccaggtcg cggtg
//
LOCUS       DAOQJH010000281         3343 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_18462_length_3343_cov_5.162409, whole genome shotgun sequence.
ACCESSION   DAOQJH010000281 DAOQJH010000000
VERSION     DAOQJH010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3343)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3343)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3343
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18462_length_3343_cov_5.162409"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            9..1421
                     /locus_tag="PLQ18_07365"
     CDS             9..1421
                     /locus_tag="PLQ18_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008569797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNE01507.1"
                     /translation="MNAAACPTGASPLATAVPASRLARIGLALRRHGRTIRMIQWAVV
                     FVYLALLLIPAALPLPPEDARLFGHLGTIAQFVFWGIWWPFVLISMVLFGRVWCGVFC
                     PEGALAEQASRVGLGRGIPRWLRWQGWPFVAFVLTTVYGQLVSVYQYPLAAALVLGGS
                     TVAAIAIGLLYGRNKRVWCRHLCPVSGVFSLLARLAPVHYRSDPERWTAKAGGPVQPV
                     NCAPLLDIRRLHGAAQCHACGRCAGQRGAVELTARAPSEEIVRHGAQDATRWDLRLVL
                     YGLCGVAMGAFHWTASPWFIDAKQAVAMWLLDHGGERLLDTDAPWWLLTHQPERHDVF
                     SWLDGLMIIAYIGATGLVVGAVLHLLCAIGARLLAPRPAPTAHLSLAFVPLAGAGVFL
                     GLSALTVSLLRGDGFALYWVGPLRELLLAAAALWTLRLGWGISGRYGAGLHRRLGATL
                     LVAAGAGFVVWGWALLFWLW"
     gene            complement(1522..1986)
                     /locus_tag="PLQ18_07370"
     CDS             complement(1522..1986)
                     /locus_tag="PLQ18_07370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE01508.1"
                     /translation="MLVPMSDRLNAYFDQLVQAGYRPRYDDDGDLVFHHDGFAIGISI
                     DEEDPEFLLVFIQNFHCSGTLTMQRVACDLMADLNRRFRLAKFSRRRQEVWVSAELLL
                     ARAGDFGPALPRLIELLASAARCFRKELRERMREIEHFDHALSAAAGLMLLH"
     gene            complement(2199..2999)
                     /locus_tag="PLQ18_07375"
     CDS             complement(2199..2999)
                     /locus_tag="PLQ18_07375"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006465104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase family protein"
                     /protein_id="HNE01509.1"
                     /translation="MNPETTTSTSNAANAAPVPAARPLAGKVAFVTGGARGIGAAIAQ
                     RLAADGAAVALTWARSEAEAAATVGTIEAAGGRALALRADSADDAALEAAIERTVAQF
                     GRLDILVNSAGVLALGSIEDFPLDGLDRTLAVNVRAVVIASRIAARHLGAGGRIITIG
                     STNAERMPFAGGAVYAMSKAAVAGLTKGMARDLGARGITVNTVQPGPVDTEMNPADGP
                     MAAGLLPMLATGRYGRAEEIASFVAYLAGPEAGYITGANLMIDGGFSA"
     gene            3144..>3343
                     /locus_tag="PLQ18_07380"
     CDS             3144..>3343
                     /locus_tag="PLQ18_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_248871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNE01510.1"
                     /translation="MDTLNGLECFVRSAEARSFAAAGRRLGLTPAAVGKNVARLEATL
                     GVQLFRRSTRRLTLTEAGEDLL"
BASE COUNT          424 a         1163 c         1229 g          527 t
ORIGIN      
        1 ctgccgccat gaatgccgct gcctgcccta ccggcgcgtc gccgcttgcg accgccgtcc
       61 ccgcctcgcg cctcgcacgc atcggcctgg cgctgcgccg gcacggacgc acgatccgga
      121 tgatccagtg ggcggtggtg ttcgtgtatc tggcgctgct cttgatcccg gcggccctgc
      181 cgctgccgcc cgaggacgcg cggctgttcg ggcatctggg cacgatcgcg cagttcgtgt
      241 tctggggcat ctggtggccg ttcgtgctga tcagcatggt gctgttcggc cgggtctggt
      301 gcggcgtgtt ctgccccgag ggcgccctcg ccgagcaggc gagccgcgtc gggctcgggc
      361 gcggcatccc gcgctggctg cgctggcagg gctggccgtt cgtcgccttc gtgctgacga
      421 cggtctacgg gcagctggtc agcgtctacc agtacccgct ggccgcggcg ctggtgctcg
      481 gcggctcgac ggtcgcagcg atcgcgatcg gcctgctgta cgggcgcaac aagcgcgtct
      541 ggtgccggca tctgtgcccg gtgagcggcg tgttctcgct gctcgcacgg ctggcgccgg
      601 tgcactatcg cagtgacccc gaacgctgga cggcgaaggc cggcgggccg gtgcagccgg
      661 tcaactgcgc gccgctgctc gacatccgcc gcctgcacgg tgctgcccag tgccatgcct
      721 gcgggcgctg cgccggccag cgcggcgcgg tcgagctgac ggcgcgcgca ccgtccgagg
      781 agatcgtccg ccacggtgcg caggatgcga cgcgctggga tctgcgcctg gtgctgtacg
      841 ggctctgcgg cgtggcgatg ggcgccttcc actggaccgc gagcccgtgg ttcatcgacg
      901 ccaagcaggc cgtggcgatg tggctgctcg atcacggcgg cgagcgcctg ctcgacaccg
      961 atgcgccgtg gtggctgctg acgcaccagc ccgagcgcca cgacgtgttc tcgtggctcg
     1021 acggcctgat gatcattgcc tacatcggcg ccaccggcct cgtggtcggc gccgtgctgc
     1081 atctgctgtg cgccatcggc gcgcggctgc tggcgccgcg cccggcgccg accgcgcacc
     1141 tgtcgctcgc cttcgtgccg ctcgccgggg ccggcgtgtt cctcggcctg tcggcgctga
     1201 ccgtgtcgct gctgcgcggc gacggcttcg cgctgtactg ggtcggcccg ctgcgcgagc
     1261 tgctgctcgc cgcggccgcg ctctggacgc tgcgcctcgg ctggggcatc agcggccgct
     1321 acggcgccgg cctgcatcgc cggctcgggg cgacgctgct ggtcgcggcc ggggccggct
     1381 tcgtggtctg gggctgggcg ctgctgttct ggctgtggtg agggcactgg cgccgcgctc
     1441 ctgaaccgcg gcccgtgccg ccggaaacga aaacgccgcc ttgcgcaaag gcggcgtttt
     1501 caccggtccg gcgacggatg ttcagtgcag cagcatcagc ccggccgcgg cgctgagcgc
     1561 gtggtcgaag tgctcgatct cgcgcatgcg ctcgcgcagt tccttgcgga agcagcgtgc
     1621 ggcgctcgcc agcagctcga tcagccgcgg cagcgccgga ccgaaatccc cggcgcgcgc
     1681 gagcagcaac tcggcgctga cccagacctc ctggcgacga cggctgaact tggcgagccg
     1741 gaaacggcgg ttgaggtcgg ccatcagatc gcaggcgacg cgctgcatcg tcagcgtgcc
     1801 cgagcagtgg aagttctgga tgaacaccag caggaactcc ggatcctcct catcgatgct
     1861 gatgccgatc gcaaagccgt cgtgatggaa gacgaggtcg ccgtcgtcgt cgtagcgcgg
     1921 acggtagccg gcctgcacga gctgatcgaa gtaggcgttc aggcggtcag acatcggcac
     1981 gagcatggga gcggctccgg acagcgacag cggcgagacg ttcgcatatt acgtacagtt
     2041 aatattgatt gcgcaagtcc cgcagacagt ccgtaacccc gtgcaacgat tgacgatttg
     2101 ctgccacccg ccatacgtcc tgccggtggt ggcttctgac gggaccgacc cggccccgcc
     2161 cggcgcccgc gctgccggac ggggaccggc ccggtgcttc aggccgagaa gccgccgtcg
     2221 atcatcaggt tcgcgccggt gatgtagccg gcctcgggac cggcgagata ggcgacgaag
     2281 ctcgcgatct cctcggcacg gccgtagcgg ccggtcgcga gcatcggcag cagccccgcc
     2341 gccatcgggc cgtcggccgg attcatctcg gtgtcgaccg ggccgggctg gacggtgttg
     2401 acggtgatgc cgcgcgcgcc gagatcgcgc gccatgccct tggtcagccc ggcgaccgcg
     2461 gccttgctca tcgcgtacac ggcaccgccg gcaaacggca tgcgctcggc gttggtgctg
     2521 ccgatcgtga tgatgcggcc gccggcgccg aggtgacgcg cggcgatgcg gctggcgatg
     2581 acgacggcgc gcacgttgac cgcgagcgtg cggtcgagcc cgtcgagcgg gaagtcctcg
     2641 atcgaaccga gcgcgagcac gccggcgctg ttgaccagga tgtcgagccg cccgaactgc
     2701 gcgaccgtgc gctcgatcgc ggcctcgaga gcggcatcgt cggcgctgtc ggcacgcagc
     2761 gccagcgccc ggccgccggc cgcctcgatc gtccctaccg tcgccgcagc ctcggcctcg
     2821 gaacgcgccc aggtcagcgc cacggcggcg ccatcggcgg cgaggcgctg cgcgatcgca
     2881 gcaccgatgc cgcgcgcgcc gccggtcacg aaggcgacct tgccggccag cgggcgggca
     2941 gcggggaccg gagcggcatt ggcggcattg ctggtgctgg tcgtggtttc aggattcatg
     3001 tcggactccg ctctgctggg ggtggctggg aacgaggacc agcatgcgcg tccgattagc
     3061 gactgattag tcggcaaaac cgcggttcag tcgataccgg tggtttacgc tggaaccctg
     3121 acttcgcagc cgcgggcacg acgatggata cgctgaacgg cctcgaatgc ttcgtgcgca
     3181 gcgccgaggc ccgcagcttt gcggcggccg gccgccggct cgggctgacg ccggccgcgg
     3241 tcggcaagaa cgtcgcgcgc ctcgaagcca cgctcggcgt gcagctgttc cggcgcagca
     3301 cgcggcggct gacgctgacc gaggccggcg aggatctgct gca
//
LOCUS       DAOQJH010000282         3343 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_18466_length_3343_cov_4.405718, whole genome shotgun sequence.
ACCESSION   DAOQJH010000282 DAOQJH010000000
VERSION     DAOQJH010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3343)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3343)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3343
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18466_length_3343_cov_4.405718"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..696
                     /locus_tag="PLQ18_07385"
     CDS             <1..696
                     /locus_tag="PLQ18_07385"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015281793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HNE01511.1"
                     /translation="WLPKVPPGHPCGRLHGHGFEVVLHANQDLGSADMGIDQDGLAVL
                     WAPLGAKLDHACLNDLPGLDCPTSEVLARWIWQRLKPRQPELSWVTVFETVTAGCQYD
                     GEHFRIWKEQRFEAATRLSCAPVEDRRAGLHGHSYAIRLHVDAPLDEVLGWTVDFGDV
                     KARFAPVYDRLDHQRLDALPGLADGDTAALAAWIRAEAAGALPELDRIDLYETPGNGV
                     ALCWGALAPALPG"
     gene            710..1351
                     /gene="queE"
                     /locus_tag="PLQ18_07390"
     CDS             710..1351
                     /gene="queE"
                     /locus_tag="PLQ18_07390"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015821849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase"
                     /protein_id="HNE01512.1"
                     /translation="MAYTVKELFYTLQGEGAQTGRAAVFCRFAGCNLWSGREADRARA
                     VCRFCDTDFLGTDGEGGGRFADAEALAQAVAARWPQGAGGRPYVVCTGGEPLLQLDAP
                     LIDALHARGFEVAVETNGTQPAPAGLDWICVSPKAGAPLVLTAGDELKLVYPQVEIEA
                     QPERFAALAFRHFFVQPLDGPERADALAACIDYCQRHPQWRLSLQTHKLIGIP"
     gene            1519..2412
                     /locus_tag="PLQ18_07395"
     CDS             1519..2412
                     /locus_tag="PLQ18_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014993458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNE01513.1"
                     /translation="MSVPEVESFFEPFTETWSHLLIAPEGCCAILDPVLDYDYRSGRT
                     GTASAGRIAARVRERGLTVEWILETHVHADHLSAGDHLRRTLGGRLAIGEHVCEVQQR
                     FARLYGAGADFRSDGSQFDQLFTDGERFRLGSLEIEVLHGPGHTPACVAYRVGTAVFV
                     GDLIFMPDVGTARCDFPGGSARALYRSVRRILDLPPDTVLYFCHDYPPEGRERSAYAS
                     VAAQRAANIDIHDGIGEDEFVARRLARDAGMPLPRLMLPALQVNMRAGALPPAEADGV
                     QYLKIPLNVLGAPDAADDPQP"
     gene            2409..>3343
                     /locus_tag="PLQ18_07400"
     CDS             2409..>3343
                     /locus_tag="PLQ18_07400"
                     /inference="COORDINATES: protein motif:HMM:NF013109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HNE01514.1"
                     /translation="MNPGRRTGFWLPWLAVSRAEGAAGLVADAIAGLVVAILLVPQAL
                     AYAQLAALPPQTGLYAGLLAPLAYALCGASRVLAVGPVAVVSLMTAAAVGPVVAATGA
                     EPQAVALALALAVGLFSLGFALLGLGALAHFLSHPVIAGLMSASALLIAVGQARHLLG
                     IAVDGATLPVLLPGLLTRLDQVNPLALAFGAGAIALLVLAGRGLAPLLQRLGLAGPTA
                     RLLARLAPVFVVLLAIGLVRGFGLEHALRTVGDIPPGLPGLTLPPADPAVWRAIAWPA
                     LLIAVIGFVESMAMAQTFAAPRRERIDPAQELRGLG"
BASE COUNT          398 a         1245 c         1198 g          502 t
ORIGIN      
        1 tggctgccga aggtgccgcc cggccatccg tgcgggcggc tgcacggcca cggtttcgag
       61 gtcgtgctgc acgccaacca ggatctcggc agcgccgaca tgggcatcga ccaggacggc
      121 ctcgccgtgc tgtgggcacc gctcggtgcc aagctcgacc acgcctgcct gaacgatctg
      181 cccggcctcg actgcccgac cagcgaggtg ctggcgcgct ggatctggca gcggctgaaa
      241 ccgcggcagc cggagctgtc gtgggtgacg gtgttcgaga ccgtcaccgc cggctgccag
      301 tacgacggcg agcacttccg catctggaag gaacagcgtt tcgaggccgc cacccggctg
      361 tcgtgcgcgc cggtggagga ccgccgcgcc ggcctgcacg gccacagcta cgcgatccgc
      421 ctgcacgtcg acgcaccgct cgacgaggtg ctcggctgga ccgtcgactt cggcgacgtg
      481 aaggcgcggt tcgcgccggt ctatgaccgg ctcgatcacc agcggctcga cgcactgccg
      541 gggctcgccg atggcgatac cgccgcgctc gccgcgtgga tccgcgccga ggccgccgga
      601 gccttgccgg aactcgatcg catcgatctc tacgagacgc ccggcaacgg cgtggcgctc
      661 tgctggggcg cgctcgcccc ggcgctgccg ggctgagcgg gcacgcacga tggcctacac
      721 ggtcaaggaa ctgttctaca cgctgcaggg cgagggcgcg cagaccggcc gcgcggcggt
      781 gttctgccgc ttcgccggct gcaatctgtg gagcgggcgc gaggccgacc gcgcgcgcgc
      841 cgtctgccgc ttctgcgaca ccgacttcct cggcaccgac ggcgaaggcg gcgggcgctt
      901 cgcggacgcc gaagccctcg cgcaggccgt cgccgcgcgc tggccgcagg gcgcgggcgg
      961 acggccctac gtggtctgca ccggcggcga accgctgctg cagctcgacg cgccgctgat
     1021 cgacgcgctg cacgcgcgcg gcttcgaggt cgcggtcgag accaacggca cgcagccggc
     1081 cccggccggc ctcgactgga tctgcgtgag ccccaaggcc ggcgcgccgc tggtgctgac
     1141 ggccggcgac gagctgaagc tcgtctaccc gcaggtggag atcgaggcac agcccgagcg
     1201 cttcgcggca ctcgccttcc ggcacttctt cgtgcagccg ctcgacggcc cggagcgcgc
     1261 agacgcgctc gccgcctgca tcgactactg ccagcgccac ccgcaatggc ggctgagcct
     1321 gcagacgcac aagctgatcg gcattccgta gcgcggccgc tcgcaccggc gagtccctga
     1381 ctgcccgatc ccgcgggcgc ggccggctgt cccttgcggc gaagaatgcc ggccggcccc
     1441 gcggcagccg gcggtgctga ctttgcgtat attcgattat ttgaatatat tcacgtcctc
     1501 cccctgccgg atgccgccat gtctgtgccc gaggtcgaaa gcttcttcga gccgttcacc
     1561 gaaacctggt cgcacctgct gatcgcgccc gaaggctgct gcgcgatcct cgatccggtg
     1621 ctcgactacg actaccgctc ggggcgcacc ggcacggcca gcgccgggcg catcgcggcg
     1681 cgcgtgcgcg aacgcgggct gacggtcgag tggatcctcg aaacccacgt gcacgccgat
     1741 cacctgtcgg ccggcgacca tctgcggcgc acgctcggcg ggcggctggc gatcggcgag
     1801 cacgtgtgcg aggtgcagca gcgcttcgcg cggctctacg gcgccggggc cgatttccgc
     1861 agcgacggat cgcagttcga ccagctgttc acggacggcg agcgtttccg gctcggctcg
     1921 ctggagatcg aggtcctgca cggtcccggc cacacgccgg cctgcgtcgc ctaccgggtc
     1981 ggcacggccg tgttcgtcgg cgacctgatc ttcatgcccg atgtcggcac cgcgcgctgc
     2041 gacttccccg gcggctcggc gcgggcgctg taccgctcgg tgcgccgcat cctcgacctg
     2101 ccgccggaca cggtcctgta cttctgccac gactatccgc ccgagggccg ggaacgctcg
     2161 gcatacgcga gcgtcgccgc acagcgcgcc gccaacatcg acatccacga cggcatcggc
     2221 gaggacgagt tcgtcgcccg gcgcctcgcc cgcgatgccg gcatgccgct gccgcggctg
     2281 atgctgccgg cgctgcaggt caacatgcgt gccggtgcgc tgccgccggc cgaagccgac
     2341 ggcgtgcagt acctgaagat tccgctgaac gtgctcggcg ccccggacgc agccgacgat
     2401 ccgcagccgt gaaccccggg cggcggaccg gattctggct gccgtggctc gccgtcagcc
     2461 gggccgaagg cgcggcgggg ctggtggccg atgcgatcgc cggccttgtg gtcgcgatcc
     2521 tgctggtgcc gcaggcgctg gcctacgcgc agctcgccgc cctgccgccg cagaccgggc
     2581 tgtatgccgg cctgctggca ccgcttgcct acgcgctctg cggcgcgagc cgcgtgctcg
     2641 cggtcgggcc ggtcgccgtc gtgtcgctga tgacggccgc cgcggtcggg ccggtggttg
     2701 ccgcgaccgg cgccgagccg caggcggtcg cgctcgcgct cgcgctcgcg gtcgggctgt
     2761 tctcgctcgg cttcgcactg ctcgggctcg gcgcgctcgc gcacttcctg agtcatccgg
     2821 tgatcgccgg cctgatgagc gcctcggcgc tgctgatcgc ggttggccag gccagacacc
     2881 tgctcggcat cgccgtcgac ggggcgacgc tgccggtgct gctgccgggt ctgctgaccc
     2941 gtctcgacca ggtgaatccg ctcgcgctcg ccttcggcgc cggcgcgatc gcgctgctgg
     3001 tgctcgccgg acgcgggctc gcccccttgc tgcagcgact cggcctcgcc ggcccgacgg
     3061 cccggctgct cgcgcggctg gcgccggtgt tcgtcgtgct gctcgcgatc gggctggtgc
     3121 gcggtttcgg gctcgaacac gcgctgcgca cggtcggcga catcccgccc ggtctgcccg
     3181 gcctgacgct gccgccggcc gatccggccg tctggcgcgc gatcgcgtgg ccggcgctgc
     3241 tgatcgcggt gatcggtttc gtcgaatcga tggcgatggc gcagaccttc gccgcgccgc
     3301 ggcgcgagcg catcgatccg gcgcaggaac tgcgcggcct cgg
//
LOCUS       DAOQJH010000283         3330 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_18583_length_3330_cov_3.779847, whole genome shotgun sequence.
ACCESSION   DAOQJH010000283 DAOQJH010000000
VERSION     DAOQJH010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3330)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3330)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3330
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18583_length_3330_cov_3.779847"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1250
                     /locus_tag="PLQ18_07405"
     CDS             <1..1250
                     /locus_tag="PLQ18_07405"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HNE01515.1"
                     /translation="MHHTRPVRRLLAAAALCVLAACASVDPKPSGPYVRQGPFPAPEN
                     LRAPVEFWKQVYGSWSLGQYALHDERYLDLVYEIVDLPGGAREGLSTDDKSVLRARRD
                     GLQAQLRELERKQLAGESLNADEKRLRERIVASSAGEAGVIGAAERLRAQRGIRERFA
                     RGLEIGARYDQRFREIFQSAGVPEDLAYLPHVESSFQASARSSVGAAGIFQFMPSTAK
                     SYMVVHTATDERLDPLVAAQGAARYLADAYSRLGDWGLAITSYNHGVGGLAKARSLHG
                     ADIGRIVWDYDGKAFGFASRNFYAQFLAARELAGRTVVTPARGIPVPRENVVLDHPTS
                     APHLASYYGLSVEQLSALNPGWSPAALGGRAMLPAGATVWVPDQTLARLAAAGQPATP
                     AELIAMADPPRTAAAPARKRGVR"
     gene            complement(1343..2020)
                     /gene="yrfG"
                     /locus_tag="PLQ18_07410"
     CDS             complement(1343..2020)
                     /gene="yrfG"
                     /locus_tag="PLQ18_07410"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014778889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HNE01516.1"
                     /translation="MASPPWTRIDTVLLDMDGTLLDLRFDNHFWLEHLPARWAALHGR
                     EPAAAREELLARYREVEGTLAWYCVEHWSRALAVDVVELKREVEHLIRFLPDSLDFLA
                     ALRQAGKRVVLATNAHPYSLALKAEKTGLGGHFDVLVSAHEFGVPKEHPKFWASLQAR
                     IGYDPPRTLFLDDSLPILRAARDYGHIGHLVSMLRPDSAQPLRARAEFAAVLRLGELL
                     PVLPSGE"
     gene            2115..2663
                     /gene="nudE"
                     /locus_tag="PLQ18_07415"
     CDS             2115..2663
                     /gene="nudE"
                     /locus_tag="PLQ18_07415"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012639467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HNE01517.1"
                     /translation="MRPLPEILATETVARSRLFRVEKVDLRFANGVEVEFERVPSGGH
                     GSVLIVPMLDADTCLLVREYAAGTHRYELGCPKGVVEAGEDPLAAANREIMEEVGHGA
                     RRLTLLRSATIAPGYFAHVTHLVLAEDLYPETRPGDEPEAIEVVPWRLSELDALLARP
                     DFTEARSIAALFLARAALAAAR"
     gene            2684..>3330
                     /gene="cysQ"
                     /locus_tag="PLQ18_07420"
     CDS             2684..>3330
                     /gene="cysQ"
                     /locus_tag="PLQ18_07420"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HNE01518.1"
                     /translation="MDTALAALAEPVLALAMRAAGEILRIYASDFAIHLKSDRTPVTA
                     ADLAAHAVLCEGLARLTPDIPVLSEESAPDEHPQRLGWPRLWLVDPLDGTREFVARSG
                     EFSINIALVEDHLPVFGLILMPVSGAAYWAWRGGGAWKLVPGSTPVPIRCRALGSGPV
                     RVAGSRRHTGHLLQTYLGYLGEHVYRGIGSALKACLIAEGEADVYPRFGLTSEWDT"
BASE COUNT          465 a         1257 c         1141 g          467 t
ORIGIN      
        1 cgatgcatca cacccgtccg gtccgccgcc tgctcgcggc agcggctctc tgcgtgcttg
       61 cggcctgcgc cagcgtcgat ccgaagccga gcggcccgta cgtgcgccag gggccgttcc
      121 cggcgcccga gaacctgcgc gcgccggtcg aattctggaa gcaggtctac ggcagctgga
      181 gcctcggcca gtacgcgctg cacgacgagc gctatctcga tctggtctac gagatcgtcg
      241 acctgcccgg cggcgcgcgc gaggggctga gcaccgatga caagtccgtg ctgcgcgccc
      301 gtcgtgacgg cctgcaggcg cagttgcgcg aactcgaacg caagcagctc gccggcgaat
      361 cgctgaacgc cgatgaaaaa cgcctgcgcg agcgcatcgt cgccagcagc gccggcgagg
      421 ccggcgtcat cggtgccgcc gagcggctgc gcgcgcagcg cggcatccgc gagcgcttcg
      481 cgcgcggcct ggagatcggc gcacgctacg accagcgctt ccgcgagatc ttccagtcgg
      541 ccggcgtccc cgaagatctc gcctacctgc cgcacgtcga atcctcgttc caggccagcg
      601 cccgctcctc ggtcggcgcc gccggcatct tccagttcat gccctcgacg gcgaagagct
      661 acatggtcgt gcacacggcc accgacgagc ggctcgatcc gctggtggcc gcgcagggtg
      721 cggcgcgcta tctcgccgat gcctacagcc ggctcggcga ctggggactc gcgatcacct
      781 cgtacaacca cggcgtcggc ggactggcca aagcgcgcag cctgcacggt gccgacatcg
      841 gccgcatcgt ctgggactac gacggcaagg ccttcggctt cgcgtcgcgc aacttctacg
      901 cgcagttcct ggccgcccgc gaactcgccg gacgcacggt cgtgacgccg gcccgcggca
      961 tcccggtgcc gcgcgaaaac gtcgtgctcg atcacccgac ctcggccccg catctggcaa
     1021 gctactacgg cctgtcggtc gagcagctct cggcgctgaa cccgggctgg tcgccggccg
     1081 ccctcggcgg ccgggcgatg ctgccggccg gcgcgacggt ctgggtgccc gatcagacgc
     1141 tcgcgcggct ggccgccgcc ggtcagccgg caacaccggc cgagctgatc gcgatggccg
     1201 acccgccgcg caccgccgct gctccggccc gcaagcgcgg cgtgcgctga tggcctgatc
     1261 cagccgcccc cggcttgtcc gaaaccgcga cggcgcgctc ccccgcgccg tcgcgcgttt
     1321 ccgggccccg gcagacccgg cctcattccc cggagggcag caccggcagc agctcgccga
     1381 gcctcagcac ggctgcgaac tcggcgcgcg cgcgcagcgg ctgcgcgctg tccgggcgca
     1441 gcatcgacac cagatggccg atgtgcccgt agtcacgcgc ggcgcgcagg atcggcaggc
     1501 tgtcgtcgag gaacagcgtg cgcggcgggt cgtagccgat gcgcgcctgc aggctggccc
     1561 agaacttcgg atgctccttc ggcacgccga attcgtgcgc gctgaccagc acgtcgaaat
     1621 gcccgccgag accggtcttc tcggccttca gcgccagact gtacggatgg gcgttggtcg
     1681 cgagcacgac gcgcttgccg gcctggcgca gtgctgcgag gaaatcgagc gaatccggca
     1741 ggaagcggat caggtgctcg acctcgcgct tcagctcgac cacgtcgacg gcgagcgccc
     1801 gcgaccagtg ctcgacgcag taccaggcca gcgtgccctc gacctcgcgg tagcgcgcca
     1861 gcagttcctc gcgggccgcg gccggctcac ggccgtgcag cgcggcccag cgcgccggca
     1921 ggtgttcgag ccagaaatgg ttgtcgaaac gcaggtcgag cagcgtgccg tccatgtcga
     1981 gcagcacggt gtcgatgcgg gtccacggcg ggcttgccat cgggtgcctc cgggggcagg
     2041 cagggggcgt ccgcccgatt atgacgcgcg cagcccgctt gtcgtcgtcg cggccgcgac
     2101 cgacaatcgc cgccatgcgc ccgctccccg aaatcctcgc gaccgaaacg gtcgcccgct
     2161 cgcgtctgtt ccgggtcgag aaggtcgacc tgcgcttcgc caacggtgtc gaggtcgaat
     2221 tcgaacgcgt gccgagcggt ggtcacggct cggtgctgat cgtgccgatg ctcgacgccg
     2281 acacctgcct gctggtgcgc gaatacgccg ccggcacgca ccgctacgaa ctcggctgcc
     2341 cgaagggcgt cgtcgaggcc ggcgaagacc cgctcgccgc cgccaaccgc gagatcatgg
     2401 aggaagtcgg ccacggcgcg cgccggctga cgctgctgcg ctcggcgacc atcgcccccg
     2461 gctacttcgc gcacgtcacg cacctcgtgc tcgccgagga cctctatccg gaaacccgcc
     2521 ccggcgacga gcccgaagcg atcgaggtgg tgccgtggcg gctgtcggag ctcgatgcgc
     2581 tgctcgcgcg gcccgacttc accgaggccc gctcgatcgc cgcgctgttt ctggcgcgcg
     2641 cggcactcgc cgcagcgcgc tgagcacccg gccggcccag ccgatggata ccgccctcgc
     2701 cgccctcgcc gaaccggtgc tggcactggc catgcgggcc gccggcgaaa tcctgcgcat
     2761 ctatgcctcc gacttcgcga ttcacctgaa gtccgaccgc acgccggtca cggcggccga
     2821 cctcgccgcg cacgccgtgc tctgcgaagg ccttgcacgc ctgacgccgg acatcccggt
     2881 gctgtccgag gaatcagcgc ccgacgaaca cccgcagcgg ctgggctggc cacggctgtg
     2941 gctcgtcgat ccgctcgacg gcacgcgcga gttcgtcgcc cgcagcggcg agttctcgat
     3001 caacatcgcg ctggtcgaag accacctccc ggtgttcgga ctgatcctga tgccggtcag
     3061 cggcgcggcc tactgggcct ggcgcggcgg cggcgcctgg aagctcgtgc cgggcagcac
     3121 gccggtgccg atccgctgcc gggcgctcgg cagcgggccg gtgcgcgtcg ccggctcgcg
     3181 ccggcatacc ggccacctgc tgcagaccta tctcggctac ctcggcgagc acgtctaccg
     3241 cggcatcggc agcgcgctga aggcctgcct gatcgccgag ggcgaggccg acgtctatcc
     3301 gcgcttcggc ctgaccagcg aatgggacac
//
LOCUS       DAOQJH010000284         3317 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_18707_length_3317_cov_4.238197, whole genome shotgun sequence.
ACCESSION   DAOQJH010000284 DAOQJH010000000
VERSION     DAOQJH010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3317
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18707_length_3317_cov_4.238197"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..424)
                     /locus_tag="PLQ18_07425"
     CDS             complement(<1..424)
                     /locus_tag="PLQ18_07425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE01519.1"
                     /translation="MPRISARLPAVAALALLAACTVTPGVISGPRGPAALPPDTVWLD
                     QNWSDEDRDLWWFTGQGSHMIPYDWFLALPGADGKPFRADPTLAGYGFLTTPATVRNP
                     DALPVGFVKDQRPATDIRGQWLGMTCAACHTAQLRIKGV"
     gene            complement(587..1318)
                     /locus_tag="PLQ18_07430"
     CDS             complement(587..1318)
                     /locus_tag="PLQ18_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010475612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HNE01520.1"
                     /translation="MPMDRNLLDIWVYLSAQPLLWLAVTLAAYLAGYRLFMASHQRAW
                     LNPVALAILLIAGLLAVTGTPYARYFEGAQFVHFLLGPATVALAVPLVRHWARIRRMW
                     LPLAVGLVAGSLTAALSAMAIAWGLGASLRTVLSMAPKSVTTPVAMGIAEKIGGLPSL
                     TAVLVILTGIVGAVLGPGLLARLGIREDEVRGYALGVAAHGIGTARAFQLGSLCGAFA
                     GLAIGLNALLTALLVPLLASMLGLS"
     gene            complement(1305..1691)
                     /locus_tag="PLQ18_07435"
     CDS             complement(1305..1691)
                     /locus_tag="PLQ18_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014241184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HNE01521.1"
                     /translation="MVPALLCLLGCQLLGEALVQLTRAPVPGPVLGMLLLALWLGLVA
                     RGRIPAALGQTADVLLAHLSLLFVPAGVGVMLHLQRIAAEWLAIVAALVLSTLLAIAA
                     TAIAMRAVLRRLPPPADDSDGRDAHG"
     gene            complement(1740..2483)
                     /locus_tag="PLQ18_07440"
     CDS             complement(1740..2483)
                     /locus_tag="PLQ18_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017716299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNE01522.1"
                     /translation="MSASTRPVAVVTGANRGLGFETSRQLARRGYLVVVTSRHPEAGE
                     VASERLQAAAAEGGGEIVLQPLDVTRPDSIAALGAFVQARCGRLDALVNNAGVLLDGH
                     GLDDATGSSVFAASLETLRTTMETNVHGPLLLAQEFVPLMRRRGYGRIVNVSSGMGQL
                     ADMGGQWPAYRLSKTALNALTRILAAETARTGILVNAVCPGWARTDMGGPKAERTPEE
                     AASGIVWAATLPDDGPSGGFFRDGKPIPW"
     gene            complement(2651..>3317)
                     /locus_tag="PLQ18_07445"
     CDS             complement(2651..>3317)
                     /locus_tag="PLQ18_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011630340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNE01523.1"
                     /translation="EVTVQYTDKNGNHELTFDKLLVSVGRRPATDGIADAAADLVLNE
                     RGFIHVDDECRTNLPGVWAIGDCVRGPMLAHKGSEEGIMVAEFIAGNHGHMNYDTIPW
                     VIYTQPEIAWVGKTEQQLKHEGVPVKIGTFPFAANGRAKAMDAAAGMVKIIAHAETDR
                     VLGAHICGPMASELIQELVVAMEFGASAEDLQMTIHGHPTLAEAVHEAALSVDKRALH
                     RVN"
BASE COUNT          511 a         1119 c         1249 g          438 t
ORIGIN      
        1 cgacgccttt gatgcgcagc tgcgcggtgt ggcaggccgc gcaggtcatg ccgagccact
       61 ggccgcggat gtcggtggcc gggcgctgat ccttgacgaa gccgaccggc agcgcgtcgg
      121 gattgcgcac ggtcgccggc gtggtcagga agccgtagcc ggcgagggtc ggatcggccc
      181 ggaacggctt gccgtcggcc ccgggcaggg cgaggaacca gtcgtacggg atcatgtgcg
      241 agccctgacc ggtgaaccac cagaggtcgc ggtcctcgtc ggaccagttc tgatcgagcc
      301 agacggtgtc gggcggcagc gcggccgggc cgcgcggacc ggagatcacg ccgggcgtga
      361 cggtgcaggc ggccagcagt gccagcgcgg caacagcggg caggcgggcg gaaatccgtg
      421 gcatcggggc ctccgaatga caggcagggc aacaggcgtg cgggtggaag gaggtgtgca
      481 gggaacggac gcattgcaat cggcgccgct gctgcgggtg gcagcgggcg cgcagtcgtg
      541 gacagggccg ggcgcggcct gcgaggaatc tagcagccgt ggccgttcag gacaggccga
      601 gcatgcttgc cagcagcggc acgagcagcg ccgtcagcag cgcgttcagc ccgatcgcga
      661 gtccggcgaa ggcgccgcac agactgccga gctggaaggc ccgcgccgtg ccgatgccgt
      721 gggcagcgac gccgagcgcg tagccgcgga cttcgtcctc gcggatgccg aggcgtgcga
      781 gcaggcccgg accgagcacg gcaccgacga tgccggtcag gatcaccagc accgcggtca
      841 gcgacggcag gccgccgatc ttctcggcga tgcccatcgc caccggcgtc gtcaccgact
      901 tcggcgccat cgacagcacc gtgcgcaggc tggcaccgag cccccaggcg atcgccatcg
      961 ccgacagcgc cgcggtcagc gagccggcga cgagcccgac cgcgagcggc agccacatcc
     1021 ggcggatgcg cgcccagtgg cgcaccagcg gcacggcgag cgcgacggtc gccgggccga
     1081 gcaggaaatg cacgaactgc gcgccttcga agtagcgcgc atacggcgtg ccggtcacgg
     1141 cgagcagccc ggcgatcagc aggatggcca gtgccaccgg gttcagccag gcgcgctggt
     1201 gcgaggccat gaacagccgg tagccggcca gatacgccgc gagcgtcacc gccagccaca
     1261 gcagcggctg cgccgacagg tagacccaga tgtcgagcag gttcctatcc atgggcatcc
     1321 ctgccgtcgc tgtcgtcggc gggcggcggc agccggcgca gcaccgcgcg catcgcgatg
     1381 gcggtcgcgg cgatcgcgag cagcgtgctc agcaccagcg ccgcgacgat cgccagccat
     1441 tcggcggcga tgcgctgcag atgcagcatc acgccgacgc cggccggcac gaacagcagc
     1501 gacaggtgcg caagcagcac gtcggcggtc tggccgagcg ccgcggggat gcgcccgcgc
     1561 gcgaccagac cgagccagag ggcgagcagc agcatgccga gcaccggacc ggggaccggt
     1621 gcacgcgtca gctgcaccag cgcctcaccg agcagctgac agccgagcag acagagcagg
     1681 gcgggaacca cggcgatcgg gactccggcg gcgggcggga gattgcgggc gcggcgcgct
     1741 caccacggga tcggcttgcc gtcgcggaag aagccgccgc tcgggccgtc gtcgggcagc
     1801 gtcgccgccc agacgatgcc ggaggcggcc tcctcgggcg tgcgctcggc cttcgggcca
     1861 cccatgtcgg tgcgcgccca gcccggacac acggcgttga cgaggatgcc ggtgcgggcg
     1921 gtctcggcgg cgaggatgcg cgtcagcgcg ttcagcgccg tcttcgacag ccggtaggcc
     1981 ggccactggc cgcccatgtc agcgagctgg cccatgccgc tcgatacatt gacgatgcgg
     2041 ccgtagccgc gccggcgcat cagcggcacg aactcctgcg cgagcagcag cgggccgtgg
     2101 acgttggttt ccatggtcgt gcgcagggtt tcgaggctgg cggcgaacac gctgctgccg
     2161 gtggcatcgt cgaggccgtg gccgtcgagc agcacgccgg cgttgttgac cagcgcatcg
     2221 agccggccgc agcgggcctg cacgaaggcg ccgagcgcgg cgatgctgtc gggtcgcgtc
     2281 acgtcgagcg gctgcagcac gatctcgccg ccgccctcgg cggccgcggc ctgcaggcgt
     2341 tcgcttgcga cctcgccggc ctcgggatgg cggctggtga cgacgaccag atagccgcgc
     2401 cgggcgagct ggcgcgaggt ttcgaatccg agtccgcggt tggcaccggt gacgaccgcc
     2461 accggccggg tgctggcact catggtcgga atctcctgcc ggtgcgggtc tgcgccggct
     2521 cctgtgtgtc cgattgtgga cggcatgcac ggcgaatgcc acgcatcgtc gcaagcaccg
     2581 gacatcgggg gagggccgga aaagacagac cccgccagaa ggcggggtcc ggggtggcgg
     2641 cgggctgcgc tcagttcacg cggtgcaatg cacgcttgtc gaccgacagc gcggcctcat
     2701 gcacggcttc ggccagcgtc ggatgaccgt ggatggtcat ctgcaggtcc tcggcgctcg
     2761 cgccgaattc catcgcgacc accagttcct ggatcagctc cgaggccatc ggcccgcaga
     2821 tgtgcgcacc gagcacgcgg tcggtttcgg cgtgggcgat gatcttgacc atgccggccg
     2881 cggcatccat ggccttggcg cgaccgttgg cagcgaacgg gaaggtgccg atcttgaccg
     2941 gcacgccctc gtgcttgagc tgctgttcgg tcttgccgac ccaggcgatc tcgggctgcg
     3001 tgtagatcac ccacgggatg gtgtcgtagt tcatgtggcc gtgattaccg gcgatgaact
     3061 cggcgaccat gatgccttcc tcgctgccct tgtgcgcgag catcgggccg cgcacgcagt
     3121 caccgatcgc ccagacaccg ggcaggttgg tgcggcactc gtcatcgacg tggatgaagc
     3181 cgcgctcgtt cagcaccaga tcggcggcgg catcggcgat gccgtcggtg gccgggcgcc
     3241 ggccgaccga gaccagcagc ttgtcgaagg tgagctcgtg gttgccgttc ttgtcggtgt
     3301 actggaccgt gacctcg
//
LOCUS       DAOQJH010000285         3315 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_18734_length_3315_cov_4.983436, whole genome shotgun sequence.
ACCESSION   DAOQJH010000285 DAOQJH010000000
VERSION     DAOQJH010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3315
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18734_length_3315_cov_4.983436"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..749
                     /locus_tag="PLQ18_07450"
     CDS             <1..749
                     /locus_tag="PLQ18_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HNE01524.1"
                     /translation="RRTAIGAPHRTRLRELEQELEEILASEGEGSPEAERLREEIRHL
                     RFRIEAIPFIDTFDLRYNNRIRQPKPTTQAVMFCIMDVSGSMDQERKDLAKRFFTLLY
                     LFLTRNYERIEVVFIRHHTVAKEVDEQEFFYSRETGGTVVSSALSLMADIVSERYSPG
                     AWNIYAAQASDGDNWQDDSPQCREILSARIMPFVQYFAYVEITPDRHQSLWEEYQKLQ
                     TTHKQFAMQEIGGAEDIYPVFRELFKRRMA"
     gene            746..2251
                     /locus_tag="PLQ18_07455"
     CDS             746..2251
                     /locus_tag="PLQ18_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015282309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HNE01525.1"
                     /translation="MSGKRLISEGSEWTFPLLETYDREIARIASEQFRLDTFRNQIEV
                     ITAEQMMDAYSSVGMPIGYHHWSFGKQFVATEKHYRRGQMGLAYEIVINSDPCIAYLM
                     EENTMTMQALVIAHACYGHNSFFKGNYLFRAWTSADSIIDYLVFARNYITRCEERYGI
                     ETVEELLDSCHALMNHGVDRYKRPAPLSMAKEQQRQREREEYLQLQVNDLWRTVPVRA
                     GAAVVEESARFPEEPQENLLYFIEKHAPLLEPWQREIVRIVRKIGQYFYPQRQTQVMN
                     EGWATFWHYTLLNRLYEEGRVSDGFMIEFLQSHTNVVFQPEFDSPYYSGINPYALGFA
                     MMSDLRRICENPTPEDRRWFPEIAGGDWLKVLDFAMRNFKDESFIAQYLSPKLIRDFK
                     LFAVLDDDKADTLEITAIHDDDGYRKVRQSLSDQYNLGSREPNIQVWNVDRRGDRSLT
                     LRHSVYNRRPLNAGTTYEVLKHMFRLWGFTVRLEAVDENGKVSLVNEVRKH"
     gene            2375..3217
                     /gene="mepA"
                     /locus_tag="PLQ18_07460"
     CDS             2375..3217
                     /gene="mepA"
                     /locus_tag="PLQ18_07460"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012971582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HNE01526.1"
                     /translation="MLRPLLSSLLLVPALAAAGPWGEVRLPSAELPQVYGGYAAGCIA
                     GAQTLPLSGPGYQAMRPSRNRYYGHPELLRFIEQLGYTAEARRFAPVLIGDLGQPRGG
                     PMLSGHRSHQIGLDVDVWFLDAPGGRAPSGAALESLSAQTMIDPYAGRVLARFGARQR
                     ALLRAAADDPAVERIFVNPVIKQSLCRSEADRGWLHKIRPWWGHDAHFHVRLYCPPDS
                     PQCSAQKPIEPGDGCDAGLDAWVEEIRQAARRPPLPTPPAPEPVLPEACTAVLQAPSA
                     SARR"
BASE COUNT          591 a         1126 c         1047 g          551 t
ORIGIN      
        1 cgcggcgcac cgcgatcggc gcgccgcacc gcacgcggct gcgcgaactc gaacaggaac
       61 tcgaagaaat cctcgccagc gagggcgaag gttcgccgga ggccgaacgc ctgcgcgagg
      121 aaatccgtca cctgcgcttc cgcatcgaag ccattccgtt catcgacacc ttcgacctgc
      181 gttacaacaa ccgcatccgc cagccgaaac cgacgacgca ggcggtgatg ttctgcatca
      241 tggacgtgtc cggctcgatg gatcaggaac gcaaggatct ggccaagcgt ttcttcacgc
      301 tgctgtatct gttcctgacc cgcaattacg aacgcatcga ggtggtgttc atccgccacc
      361 atacggtcgc caaggaagtc gacgagcagg agttcttcta ttcccgtgaa accggcggca
      421 ccgtggtgtc ctcggcgctg tcgctgatgg cggacatcgt ttcggagcgt tattcgccgg
      481 gcgcgtggaa catctacgcg gcccaggctt ccgacggcga caactggcag gatgactcgc
      541 cgcagtgccg ggaaatcctc agcgcgcgga tcatgccgtt cgtgcagtat tttgcctacg
      601 tcgagatcac acccgaccgg catcagagcc tgtgggagga gtaccagaag ctccagacca
      661 cccacaagca gttcgcgatg caggaaatcg gcggagccga agacatctat ccggtattcc
      721 gggaactgtt caagaggcgc atggcatgag tggcaaacgg ctgatttccg aaggctcgga
      781 gtggacattt ccactgctcg aaacctatga ccgcgaaatc gcgcgcatcg ccagcgagca
      841 gttccggctc gacaccttcc gcaaccagat cgaggtgatc accgccgagc agatgatgga
      901 tgcctattcc tcggtcggca tgccgatcgg ctaccatcac tggtcgttcg gcaagcagtt
      961 cgtcgccacc gaaaagcatt accggcgcgg tcagatgggc ctggcctacg agatcgtcat
     1021 caactccgat ccgtgcattg cctacctgat ggaagagaac acgatgacca tgcaggcgct
     1081 ggtcatcgcg catgcctgct acggtcacaa ctccttcttc aagggcaatt acctgttccg
     1141 ggcctggacc agtgccgatt cgatcatcga ttacctggtg ttcgcgcgca attacatcac
     1201 ccgctgcgag gagcgctacg gcatcgagac ggtcgaggaa ctgctcgact cctgccacgc
     1261 gctgatgaac cacggcgtcg accgctacaa gcgtccggca ccgctgtcga tggccaagga
     1321 acagcagcgc cagcgcgagc gcgaggaata cctgcagctg caggtcaacg acctctggcg
     1381 cacggtgccg gtgcgcgccg gtgcggcggt ggtcgaggaa agcgcgcgct tccccgagga
     1441 accgcaggag aacctgctgt atttcatcga gaaacacgcg ccgctgctcg aaccctggca
     1501 gcgcgagatc gtgcgcatcg tgcgcaagat cggccagtat ttctatccgc agcgccagac
     1561 ccaggtcatg aacgagggct gggcgacctt ctggcattac acgctgctga accggctgta
     1621 tgaagaaggc cgggtcagtg acggcttcat gatcgaattc ctgcagtcgc acacgaatgt
     1681 cgtgttccag ccggaattcg attcgccgta ttacagcggc atcaatccgt acgcgctcgg
     1741 cttcgcgatg atgagtgacc tgcgccgcat ctgcgaaaac ccgacgcccg aggaccggcg
     1801 ctggttcccg gaaatcgccg gcggcgactg gctgaaggtg ctcgacttcg cgatgcgcaa
     1861 cttcaaggac gagagcttca tcgcccagta cctgtcgccg aagctgatcc gtgacttcaa
     1921 gctgttcgcg gtgctcgacg acgacaaggc cgacacgctg gagatcaccg cgatccacga
     1981 cgatgacggc taccgcaagg tgcgccagtc gctgtcggac cagtacaacc tcggctcgcg
     2041 cgagccgaac atccaggtct ggaacgtcga ccgccgcggc gaccgttcgc tgacgctgcg
     2101 tcacagcgtc tacaaccgcc ggccgctgaa cgccgggacc acgtacgagg tgctcaagca
     2161 catgttccgg ctgtggggct tcacggtgcg gctcgaagcg gtcgacgaga acggcaaggt
     2221 cagcctcgtc aacgaggtgc gcaagcactg agccggacgc acgccctccc gccctgccgc
     2281 cgacgccgcg ccgagtgcgc ggcgtcgcct tgttgcaccg gcgccgatct tccgtagact
     2341 cgcgccccgc cccgccgagg actgtccgcc cctcatgctg cgtccgctgc tgtcgtccct
     2401 gttgctcgtc cctgccctcg ccgctgccgg cccctggggc gaagtccgcc tgccgagtgc
     2461 cgagctgccg caggtctacg gtggttatgc cgccggctgc atcgccggcg cgcagacgct
     2521 gccgctgtcc ggccccggct atcaggcgat gcgcccgtcg cgcaaccgct actacgggca
     2581 tccggagctg ctgcgcttca tcgaacagct cggctacacg gccgaggccc gccgcttcgc
     2641 gccggtgctg atcggtgatc tcggccagcc gcgcggcggg ccgatgctct cgggtcaccg
     2701 cagccaccag atcggcctcg atgtcgatgt ctggttcctc gatgcgcccg gtggccgcgc
     2761 accgtccgga gcagccctcg aaagcctgag cgcgcagacg atgatcgacc cgtacgccgg
     2821 gcgcgtgctc gcgcgcttcg gtgcccgcca gcgcgcactg ctgcgcgcgg cggccgacga
     2881 tccggcggtc gagcgcatct tcgtcaatcc ggtgatcaag cagagcctct gccgcagcga
     2941 ggccgatcgc ggctggctgc acaagatccg gccgtggtgg ggacacgatg cgcacttcca
     3001 cgtgcgcctg tactgcccgc cggacagccc gcaatgctcg gcgcagaagc cgatcgaacc
     3061 cggtgacggc tgcgatgccg ggctcgatgc ctgggtcgag gaaatccgtc aggccgcgcg
     3121 ccgcccgccg ctgccgacgc cgccggcacc cgaaccggtg ctgccggagg cctgcacggc
     3181 ggtgctgcag gcaccgtcgg caagcgcgag gcgctgatgc gaggccggct gaagctcgtg
     3241 ctgctcgccg gcttcgccgc cggcggctgc gcgtcgctgc cggcgggtct ggcgccgacc
     3301 tcggccgagc atgcg
//
LOCUS       DAOQJH010000286         3313 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_18757_length_3313_cov_3.490485, whole genome shotgun sequence.
ACCESSION   DAOQJH010000286 DAOQJH010000000
VERSION     DAOQJH010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3313
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18757_length_3313_cov_3.490485"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            224..1549
                     /locus_tag="PLQ18_07465"
     CDS             224..1549
                     /locus_tag="PLQ18_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRZ/ATZ family hydrolase"
                     /protein_id="HNE01527.1"
                     /translation="MQSIDTLLFPRWIVPVEPEAVVLDDMALAIDQGRILDLLPAAAA
                     RNRYHARNTLLLPNHCLLPGLVNAHTHLAMNLLRGYADDLPLMQWLTGHIWPAEARWV
                     GETFVHDGTLLAAAELIRGGVTCVNDMYFFPDVTARALGTAGLRAVVGLIAIDFPSAW
                     ASGPDEYLAKGLALHESLRHDPLLSTAFAPHAPYTVGEATLARIRALADELERPVHMH
                     VHETAGEIREAVAAHGCRPLARLDRLGLLSPALAAVHMTQLEDAEIARLAETGASVIH
                     CPESNLKLASGFCPVAKLEAAGVNVALGTDGAASNNDLDLFGEMRTAALLAKGVSGDA
                     AALPAHRALRMATLNGARALGLGEQTGSLVPGKWADVIAVDLGDLESRPLYHPISQLV
                     YASGRHQVTDVWVAGRQLLGNRELLTLDRSALLERAGHWRAQIGAGASA"
     gene            1585..2292
                     /gene="ubiG"
                     /locus_tag="PLQ18_07470"
     CDS             1585..2292
                     /gene="ubiG"
                     /locus_tag="PLQ18_07470"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014700795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008689 - 3-demethylubiquinone-9
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HNE01528.1"
                     /translation="MTTANVDQAEVAKFDALASRWWDPDGEFKPLHDINPLRLGFIKS
                     RAPLTDCAVLDVGCGGGVLSEALADEYAQVTGIDMAAAPLEIASLHLIESGLDVDYVQ
                     STAEDYADAHPDAFDVVTCMEMLEHVPEPASTIAACARLVRPGGHVFFSTLNRTPKAW
                     LLAVLGAEYLLRMLPRGTHEYAKFIRPSELARWAQLAGLEVREFTGVHYNPLSRRYTL
                     GPGLDVNYLVHCVRPQD"
     gene            2354..3025
                     /locus_tag="PLQ18_07475"
     CDS             2354..3025
                     /locus_tag="PLQ18_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011628658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HNE01529.1"
                     /translation="MTAILFDLDGTLLDTAPDLVGALNRMRIEDGLPALDYASLRPAA
                     SHGTDALLAAGYGSTRHDAGHAALRERYLAQYRARIAERTAPFPGMDDTLATLEARGI
                     AWGIVTNKPSALTGALLAALGLDARACAVVSGDTLAVQKPDPAPLLHACTLAGLDPAR
                     CWYIGDAARDIEAGSRAGMRTLVAGWGYLGPDDEPAAWGADGTLAVPTELLDWLDPGP
                     EAAHD"
     gene            3018..>3313
                     /locus_tag="PLQ18_07480"
     CDS             3018..>3313
                     /locus_tag="PLQ18_07480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE01530.1"
                     /translation="MTETPDDAGLAGRLAGLLPGALGGWLPAPVLADWSALATLSRTL
                     AGLEDSEARAEAIAALPPALARLAAADWWRPLPCGPVEDVATLLAEAQRAWRPYA"
BASE COUNT          461 a         1281 c         1118 g          453 t
ORIGIN      
        1 gttgccgaca acgcgacggc cgccggtcgc gcgcagaacc gccgcgtcga gatcaccgtc
       61 gaagtcacgc agtaagcttc gacctcgcct cgcacacccc gcacgccccg gccatcggcc
      121 ggggcgtcgt catttggccg gccgccggcg gatgcagaac cgctgccgca ggcacttcgc
      181 agaaccggca taatcgctgc catgcgccga gccggagccc tccatgcagt ccatcgacac
      241 cctgttgttt ccgcgctgga tcgtgccggt cgagcccgaa gcggtcgtgc tcgacgacat
      301 ggcgctcgcg atcgaccagg gccgcatcct cgacctgctg ccggccgctg ccgcgcgcaa
      361 ccggtatcac gcgcgcaaca cgctgctgct gccgaatcac tgcctgctgc cgggcctcgt
      421 caacgcgcac acgcatctgg cgatgaacct gctgcgcggc tacgccgatg acctgccgct
      481 gatgcagtgg ctgaccggcc acatctggcc ggccgaggcg cgctgggtcg gcgagacctt
      541 cgtgcatgac ggcacgctgc tcgccgcggc cgagctgatc cgcggcggcg tcacctgcgt
      601 caacgacatg tatttcttcc cggacgtgac ggcgcgcgcg ctcggcacgg ccggcctgcg
      661 cgccgtggtc ggcctgatcg ccatcgactt cccgagcgcc tgggcctcgg ggccggacga
      721 atacctcgcc aagggcctgg cgctgcacga atccctgcgc cacgacccgc tgctgagcac
      781 cgcgttcgcg ccgcacgcgc cgtacacggt cggcgaggcc acgctcgcac gcatccgcgc
      841 gctggccgac gagctcgaac gcccggtgca catgcacgtg cacgagaccg ccggcgagat
      901 ccgggaggcc gtcgccgcgc acggctgccg gccgctggcg cgcctcgacc gcctcggact
      961 gctgtcgccg gccttggccg ccgtgcacat gacccagctc gaagacgccg agatcgcccg
     1021 cctcgccgag accggcgcca gcgtcatcca ctgccccgaa tcgaacctga agcttgcctc
     1081 cggcttctgc ccggtcgcga agctcgaagc tgccggcgtc aacgtcgcgc tcggcaccga
     1141 cggcgcggcc agcaacaacg atctcgacct gttcggcgag atgcgcaccg ccgccctgct
     1201 cgccaagggc gtcagcggcg atgccgcggc gctgccggcg caccgggcgc tgcgcatggc
     1261 gacgctcaac ggcgcacgcg cgctcgggct cggcgagcag accggctcgc tggtgccggg
     1321 caagtgggcc gatgtcatcg ccgtcgatct cggcgatctc gaaagccggc cgctgtatca
     1381 cccgatctcg cagctcgtct acgcgagcgg ccgccaccag gtcaccgatg tctgggtcgc
     1441 cggccgccag ttgctcggca accgcgagct gctgacgctg gatcgcagcg cgctgctcga
     1501 acgcgccggg cactggcgcg cgcagatcgg tgccggcgcg agcgcctgaa cccgcaagtt
     1561 tccgccttga ccgaggaacg ccgcatgacg accgccaacg tcgatcaggc cgaagtcgcc
     1621 aagttcgatg ccctcgccag ccgctggtgg gatccggacg gcgagttcaa gccgctgcac
     1681 gacatcaacc cgctgcgcct cggcttcatc aagtcgcgcg ccccgctgac ggactgcgcg
     1741 gtgctcgatg tcggctgcgg cggcggcgtg ctgagcgaag ccctcgccga tgaatacgcg
     1801 caggtcaccg gcatcgacat ggccgctgca ccgctcgaaa tcgccagcct gcacctgatc
     1861 gaatccggac tcgatgtcga ttacgtgcag tcgacggccg aggactacgc cgacgcgcat
     1921 ccggacgcct tcgacgtggt cacctgcatg gagatgctcg aacacgtgcc ggaaccggcc
     1981 tcgaccatcg ccgcctgcgc gcggctggtg cgcccgggcg ggcacgtgtt cttctcgacg
     2041 ctgaaccgca cgccgaaggc ctggctgctg gccgtgctcg gggccgaata cctgctgcgc
     2101 atgctgccgc gcggcacgca cgagtacgcg aagttcatcc gcccctcgga gctggcacgc
     2161 tgggcgcagc tcgccgggct ggaggtgcgc gagttcaccg gcgtgcacta caacccgctg
     2221 tcgcgccgct acacgctcgg ccccgggctc gacgtgaact acctcgtgca ctgcgtgcgg
     2281 ccgcaggact gataccggtc acgaccgaac gcccgagcct ttcagcccct cccctgacga
     2341 cggaagcccc cgcttgaccg ccatcctgtt cgatctcgac ggcacgctgc tcgacaccgc
     2401 gccggatctg gtcggcgcgc tgaaccgcat gcgcatcgag gacggcctgc cggccctcga
     2461 ctacgccagc ctgcgcccgg ccgcctcgca cggcaccgac gcgctgcttg cggccggcta
     2521 cggcagcacg cgccatgacg ccggccacgc cgcgctgcgc gagcgctatc tcgcgcagta
     2581 ccgcgcacgc atcgccgagc gcaccgcgcc gtttcccggc atggacgaca cgctggccac
     2641 gctggaggca cgcggcattg cctggggcat cgtcaccaac aagccctcgg cgctgaccgg
     2701 cgcgctgctc gcggcgctcg gcctggacgc ccgcgcctgc gcggtggtca gcggcgacac
     2761 gctggcggtg cagaagcccg atccggcgcc gctgctgcac gcctgcacgc tcgccggtct
     2821 cgatccggcg cgctgctggt acatcggcga cgccgcgcgc gacatcgagg ccggcagccg
     2881 cgccggcatg cgcacgctgg tggccggctg gggctacctc ggcccggacg acgaacccgc
     2941 ggcctggggc gccgacggca cgctcgccgt gccgaccgaa ctgctcgact ggctggaccc
     3001 cggtccggag gccgcccatg actgaaacgc cggacgatgc cggacttgcc gggcgcctcg
     3061 ccggcctgct gcccggagcg ctcggcggct ggctgccggc gccggtgctc gcggactggt
     3121 cggcgctggc cacgctcagc cgcacgctgg caggcctgga ggattccgag gcccgcgccg
     3181 aggcgatcgc ggcactgccg ccggcgctgg cccgcctcgc cgccgccgac tggtggcgac
     3241 cgctgccgtg cggtccggtc gaggacgtgg cgacgctgct cgccgaggcg cagcgcgcgt
     3301 ggcgcccgta tgc
//
LOCUS       DAOQJH010000287         3308 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_18803_length_3308_cov_4.012604, whole genome shotgun sequence.
ACCESSION   DAOQJH010000287 DAOQJH010000000
VERSION     DAOQJH010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3308)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3308)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3308
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18803_length_3308_cov_4.012604"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>3308
                     /locus_tag="PLQ18_07485"
     CDS             <1..>3308
                     /locus_tag="PLQ18_07485"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HNE01531.1"
                     /translation="VYVIAPNCCLEYQNGRLVPREPETPRWLRKPIDVLLRSLARSCG
                     ARAIGVVFSGGGSDGTLGLKDIRNAGGLVLVQTPETAMQDSMPTSAIDAGLDDHVLAI
                     SAMPAVLIEHVQHRWHSEPVPPEPEPAEAEPEPEPEDADREKPDLEDVLALLRARQDF
                     DFRCYKKGMLARRVQRRMSALRLRESKDYLALLQRDSAEFDVLAADLMISVTQFFRNP
                     QAWDHLAEQVLPELCELASAERPLRAWVPACASGEEVYSLAILLLERLLPGQPHIQLF
                     ASDSSEAALNVARTGLYPDSITAHVTPARLARFFRAEGTRYRVIREVRDCVTFAVQNL
                     INDPPFGRIDLISCRNFMIYLEPEMQERMLALFHFALRPGGYLFLGNVESIGGQFDLF
                     EPVSHKWRVYRRIGTPRLARDGYPPTLRRPSGVQNWAVRLDAPRKAGQIPAAMAAPEL
                     SGAGPLLGAKVSGIAGYAQQIVLERYTRACVLVNRRYEILYFFGPTHEFLRQPVGEHT
                     ADLFAWTRDGLRSMLRSALHEISTTAANRVVRQHAAEAAGSGDIECIVEALRVPRELD
                     GLLLIAFTRLPVVGPPPREVAGTADASGTAIAQLEFELARAREDLSGMTEQLESAYEE
                     FTASNEEVMSINEELQSANEELETSKEELQSLNEELVTSNQQLETKNQQLEEINADLR
                     NLLASTQIATLFLDEHFCIRRYTPAMAQLMRVIPSDIGRSVEDISCDFDRVALLAQSV
                     RVLEHLAPIETEIRTYDGRWHLCRVLPYRTEDNRIGGVVVTFTDVSVRHRLEVRERLM
                     FALNDRLAELQDPADIVTAVSELIERHLGAAWSRYAPVDSAQQWLYVEGSSVGARVSL
                     TRLPADVLRTLRRGQPLISTGNAGADPGLHAALTTLGAPQQLPGTVLLFAPVVDGRLL
                     TLVGVGHTGVYLWTDEDLDLVSVLAERTWIAIEKIEVNRTLVRRATQLAATDRLKNEF
                     LATLGHELRNPLVPIRSTLDLLLGADQEGQLQAQLQLVDRQVQQLQRLVDDLLDASRI
                     EQIGQIRLRMQDLDLDDVLQMAIEGQQPRFVARRQDFSYLPPSSPLALVGDRVRLVQA
                     VGNLLHNASRYSPA"
BASE COUNT          512 a         1192 c         1126 g          478 t
ORIGIN      
        1 acgtctacgt gatcgcgccg aactgctgcc tcgaatacca gaacggccgg ctcgtgccgc
       61 gggagccgga aaccccgcgc tggctgcgca agcccatcga cgtgctgctg cgctcgctcg
      121 cacgcagctg cggcgcgcgc gcgatcggcg tggtcttctc gggcggcggc agcgacggca
      181 cgctcggcct caaggacatc cgcaacgcgg gcgggctcgt gctggtgcag acgcccgaga
      241 ccgcgatgca ggacagcatg ccgaccagcg cgatcgacgc cggtctcgac gatcacgtgc
      301 tggcgatctc cgccatgccg gcggtgctga tcgagcatgt gcagcatcgc tggcacagcg
      361 agcccgtgcc gccggagccg gaaccggccg aagcggagcc ggagccggag ccggaggatg
      421 cggatcggga gaagccggat ctggaggatg tgctggcgct gctgcgcgcg cgccaggact
      481 tcgacttccg ctgctacaag aaaggcatgc tggcgcggcg cgtccagcgg cgcatgagcg
      541 cgctgcgcct gcgcgagagc aaggactatc tcgcgctgct gcagcgtgac tccgcggagt
      601 tcgatgtgct ggccgccgat ctgatgatca gcgtcacgca gttcttccgc aacccgcagg
      661 cctgggacca tctggccgag caggtgctgc ccgagctctg cgagctggcc tcggccgagc
      721 ggccgctgcg ggcctgggtg ccggcctgcg cgagcggcga ggaggtctac agcctcgcga
      781 tcctgctgct ggagcgcctg ctgccggggc agccgcacat ccagctgttc gcttcggatt
      841 cgagcgaggc cgcgctcaac gtcgcgcgca ccggcctcta tccggacagc atcacggcgc
      901 atgtgacgcc agcgcggctg gcgcgcttct tccgcgccga gggcacgcgc taccgggtga
      961 tccgcgaggt gcgcgactgc gtgaccttcg cggtgcagaa cctcatcaac gatccgccgt
     1021 tcggccgcat cgacctgatc agctgccgca acttcatgat ctatctcgaa ccggagatgc
     1081 aggaacgcat gctggcgctg tttcacttcg cgctgcgtcc ggggggctat ctgttcctcg
     1141 gcaacgtcga gtcgatcggc gggcagttcg acctcttcga gccggtctcg cacaagtggc
     1201 gcgtgtaccg gcgcatcggc actccgcggc tggcgcgcga cggctacccg ccgacgctgc
     1261 gccgcccctc gggcgtgcag aactgggccg tgcgcctcga tgcgccgcgc aaggcggggc
     1321 agataccggc ggcgatggcg gcaccggagc tgagcggtgc ggggccgctg ctcggcgcca
     1381 aggtctcggg gatcgccggc tacgcgcagc agatcgtgct ggagcgctac acgcgcgcct
     1441 gcgtgctcgt caaccgccgc tacgagatcc tgtacttctt cggcccgacg cacgagttcc
     1501 tgcgccagcc ggtcggcgag cacaccgccg acctgttcgc gtggacgcgc gacggcctgc
     1561 gcagcatgtt gcgctccgcg ctgcacgaga tcagcacgac ggcggccaac cgcgtggtgc
     1621 gccagcacgc cgccgaggcc gccggcagcg gcgacatcga gtgcatcgtc gaggccctgc
     1681 gcgtgccgcg cgagctcgac ggcctgctgc tcatcgcctt cacgcgcctg cccgtcgtcg
     1741 gcccgccgcc gcgcgaggtc gccggcacgg ccgatgccag cggcaccgcg atcgcgcagc
     1801 tcgaattcga gctggcgcgc gcgcgcgagg acctctccgg catgaccgag cagctcgaaa
     1861 gcgcctacga ggagttcacg gccagcaacg aggaggtgat gtcgatcaac gaggagctgc
     1921 agtcggccaa cgaggagctc gaaacctcga aggaggagct gcagtcgctg aacgaggaac
     1981 tggtcaccag caaccagcag ctcgaaacca agaaccagca actggaggag atcaacgccg
     2041 atctgcgcaa cctgctcgcg agcacgcaga tcgcgacgct gttcctcgac gagcacttct
     2101 gcatccgccg ctacacgccg gcgatggcgc agctgatgcg cgtgatcccg agcgacatcg
     2161 gccgctcggt cgaggacatc agctgcgact tcgaccgcgt ggcgctgctc gcgcagtccg
     2221 tgcgcgtgct cgaacacctc gcaccgatcg aaaccgagat ccgcacctat gacgggcgct
     2281 ggcatctgtg ccgcgtgctg ccgtaccgca ccgaggacaa ccgcatcggc ggcgtcgtcg
     2341 tcaccttcac cgacgtgagc gtgcgccatc gcctggaagt gcgcgaacgg ctgatgttcg
     2401 ccctcaacga ccgcctcgcc gaactccagg acccggccga catcgtcacg gccgtttccg
     2461 agctgatcga gcgccatctc ggcgcggcct ggagccgcta cgccccggtc gacagcgcgc
     2521 agcagtggct gtacgtcgag ggctccagcg tcggggcgcg cgtgtcgctg acgaggctgc
     2581 cggccgacgt gctgcgcacg ctgcgccgcg gccagccgct gatcagcacc ggcaatgccg
     2641 gcgccgatcc gggcctgcac gcggcgctga cgacgctcgg cgccccgcag cagctgcccg
     2701 gcacggtgct gctgttcgcg ccggtcgtcg acggccggct gctgacgctg gtcggcgtcg
     2761 gccacaccgg cgtctatctg tggaccgacg aggatctcga cctcgtcagc gtgctcgccg
     2821 agcgcacgtg gatcgccatc gagaagatcg aggtcaaccg cacgctcgtg cgccgcgcca
     2881 cgcagctcgc ggccaccgac cggctcaaga acgagttcct cgcgaccctc ggccacgaac
     2941 tgcgcaaccc cctcgtgccg atccgcagca ccctcgacct gctgctcggc gccgatcagg
     3001 aagggcagct gcaggcgcag ctgcagctcg tcgaccgcca ggtgcagcag ctgcagcgcc
     3061 tcgtcgatga cctgctcgat gccagccgca tcgagcagat cggccagatc cgcctgcgca
     3121 tgcaggatct tgatctcgac gacgtgctgc agatggcgat cgagggccag cagccgcgct
     3181 tcgtcgcgcg ccgccaggac ttctcctacc tgccgccgtc cagcccgctc gcgctcgtcg
     3241 gcgaccgggt gcgtctggtg caggcggtcg gcaacctgct gcacaacgcc agccgctaca
     3301 gcccggcc
//
LOCUS       DAOQJH010000288         3300 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_18881_length_3300_cov_3.838829, whole genome shotgun sequence.
ACCESSION   DAOQJH010000288 DAOQJH010000000
VERSION     DAOQJH010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3300)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3300)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3300
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18881_length_3300_cov_3.838829"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1413)
                     /locus_tag="PLQ18_07490"
     CDS             complement(<1..1413)
                     /locus_tag="PLQ18_07490"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012822936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HNE01532.1"
                     /translation="MVLAGYLDPAQVEAVRSACWFSAEAHQGQYRKSGEPYVFHPIAV
                     ARILAEVHFDHETLMAAVLHDVIEDTKFGKDQVTERFGPEVAELVDGVTKLTQIQFSS
                     KLEAQAENFRKMFLAMAKDIRVIMVKLGDRLHNMRTLGAMPAEKRRRIARETLEIYAP
                     IAGRLGMNHLRLELENLGFAYLYPTRPRVLSEAVRKLRGNRREIISQIETRVRNRLLE
                     EGIKARVVGREKHLWGIYKKMHEKRLGLKEVYDVYAVRLIVDSVDTCYRTLGLVHGLY
                     KPIMGRFKDYVAIPKANGYQSLHTVLFGPHGVPIEVQIRTQDMHVMAEAGVAAHWLYK
                     SPGAQGNAAHARAREWLARLLDMQRRAGNSVEFLESVKMDLFPDEVYVFTPRGEIIEL
                     PKGATAVDFAYAIHSDVGNTCVAAKVDRRLVPLRTPLANGQTVEVIITPTARPNPAWL
                     GFVVTAKARALVWNFPDPIPQ"
     gene            complement(1546..1881)
                     /gene="rpoZ"
                     /locus_tag="PLQ18_07495"
     CDS             complement(1546..1881)
                     /gene="rpoZ"
                     /locus_tag="PLQ18_07495"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008293426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HNE01533.1"
                     /translation="MARVTVEDCLDRVENRFQLVLVASRRARQIALGQMPLVPEENDK
                     PTVIALRELAAGLIDPQALMREPQVLPEREREPVLADFSFDEPLDMAGGEPSPVTPPA
                     PGLDGEANA"
     gene            complement(2025..2642)
                     /gene="gmk"
                     /locus_tag="PLQ18_07500"
     CDS             complement(2025..2642)
                     /gene="gmk"
                     /locus_tag="PLQ18_07500"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013717758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HNE01534.1"
                     /translation="MTASGQLYIVSAPSGAGKTSLVRALLTQDAQVEVSVSHTTRAPR
                     PGERDGEHYHFVSDDAFARLVADDAFLEHAQVFDRRYGTARTGVAARLAAGRDVILEI
                     DWQGARQVRAAFPEAQSVFIVPPSVGELRRRLSGRGQDAAEVIERRMRDARSELMHWV
                     EFDYLIVNDDFGRALAELQALFTARRLRRSVQAERHGALLDALLD"
     gene            complement(2694..>3300)
                     /locus_tag="PLQ18_07505"
     CDS             complement(2694..>3300)
                     /locus_tag="PLQ18_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020395442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HNE01535.1"
                     /translation="LLGIAGELENLIGPGTGLRLADVLRWPGVVTEAEQDLEPLKRGV
                     LEVLDAALAELVATREREGARTAEMILSRCDAIAAQVAFVRQRRPEVVARLREKLTGR
                     LADLPAGADPGRLEQEMVIVAQRMDVEEELDRLDAHLAEIRDVLRRPEPVGRRLDFLM
                     QELNREANTLSSKSLDTETTRAAVELKVLIEQMREQIQNIE"
BASE COUNT          480 a         1137 c         1160 g          523 t
ORIGIN      
        1 ctgcgggatc gggtcgggga agttccacac cagggcgcgc gccttggcgg tgacgacgaa
       61 accgagccag gccggattcg ggcgcgcggt cggcgtgatg atgacctcga cggtctggcc
      121 gttggcgagc ggcgtgcgca gcggcaccag ccggcggtcg accttggcgg cgacgcaggt
      181 gttgccaaca tcggagtgga tcgcgtaggc gaagtcgact gcggtcgcgc ccttgggcag
      241 ctcgatgatc tcgccgcgcg gcgtgaacac gtagacctcg tccgggaaga gatccatctt
      301 cacgctttcg aggaactcga ccgaattgcc ggcccggcgc tgcatgtcga gcagccgtgc
      361 cagccattcg cgcgccctgg cgtgcgcggc gttgccctgc gcgcccggcg acttgtacag
      421 ccagtgcgcg gcaacaccgg cctcggccat cacgtgcatg tcctgcgtgc ggatctgcac
      481 ctcgatcggc acgccgtgcg gtccgaacag cacggtgtgc agcgactggt agccgttggc
      541 cttcgggatc gcgacgtagt ccttgaagcg gcccatgatc ggcttgtaca ggccgtgcac
      601 gaggccgagc gtgcggtagc aggtatcgac gctgtcgacg atcaggcgca cggcgtagac
      661 gtcgtagacc tccttcaggc cgaggcgctt ctcgtgcatc ttcttgtaga tgccccagag
      721 atgcttctcg cgcccgacca cgcgcgcctt gatgccctct tcgagcaggc ggttgcgcac
      781 gcgcgtctcg atctgggaaa tgatctcgcg gcggttgccg cgcagcttgc gcaccgcctc
      841 ggacagcacg cgggggcgcg tcggatacag gtacgcgaag ccgaggttct cgagttccag
      901 ccgcaggtgg ttcatgccga ggcggccggc gatcggggcg tagatctcca gggtctcgcg
      961 ggcgatgcgc cggcgcttct cggccggcat cgcgccgagc gtgcgcatgt tgtgcaggcg
     1021 gtcgccgagc ttgaccatga tcacgcggat gtccttcgcc atcgcgagga acatcttgcg
     1081 gaagttctcg gcctgcgcct ccagcttcga gctgaactgg atctgcgtga gcttggtgac
     1141 gccgtcgacc agttcggcga cctcggggcc gaagcgctcg gtgacctggt ccttgccgaa
     1201 cttggtgtcc tcgatgacgt cgtgcagcac ggccgccatc agggtctcgt ggtcgaaatg
     1261 cacctcggcg aggatgcgcg cgaccgcgat cgggtggaac acgtagggct cgccgctctt
     1321 gcggtactgc ccctggtgcg cttcggccga gaaccagcag gccgagcgca cggcctcgac
     1381 ctgtgcgggg tcgagatagc cggccagcac catgcacagc tcctcgatcc gggagttcag
     1441 ctcctcggac cagtcgctgc cgggcagcac ggcgctcgac gcgctcaccg atgcctcaac
     1501 gcgatcgctc gtgccgcagc gggcggagct tcaggccgca cgcgctcacg cgttcgcctc
     1561 gccgtcgagg ccgggggccg gcggcgtgac cggcgaaggt tcgccgccgg ccatgtccag
     1621 cggctcatcg aagctgaaat ccgccagcac cggctcgcgc tcgcgctccg gcagcacctg
     1681 cggctcgcgc atcagcgcct gcggatcgat caggccggcg gccagctcgc gcagcgcgat
     1741 gacggtcggc ttgtcgtttt cctcgggcac cagcggcatc tggccgagtg cgatctggcg
     1801 cgcgcggcgc gaggcgacca gcacgagctg gaagcggttc tcgacgcgat ccagacaatc
     1861 ttcaacggtg acgcgtgcca tgttcgggat ttcctcgaag attcaacggg tgacgacgct
     1921 gccggccgac cgtacggccc gcacgacgcg caccccggac aaaagtgagt cagtgtacgc
     1981 agacgccccc ggctgccaca accgccgtcc cggcgtgccg gccttcagtc gagcagcgca
     2041 tcgagcagcg cgccgtgacg ctcggcctgc acgctgcggc gcaggcgccg ggcggtgaac
     2101 agcgcctgca gctcggccag cgcccggccg aagtcgtcgt tgacgatcag gtaatcgaat
     2161 tcgacccagt gcatgagttc ggagcgcgca tcgcgcatgc gccgctcgat gacctcggcc
     2221 gcgtcctggc cgcggccgct caggcgccgg cgcagttccc cgaccgaggg cggcacgatg
     2281 aacaccgact gcgcttccgg aaacgcggcc cgcacctgac gcgcgccctg ccagtcgatc
     2341 tcgaggatca cgtcgcgccc ggccgcgagc cgcgccgcga caccggtgcg ggcggtgccg
     2401 tagcggcggt cgaacacctg cgcgtgttcg aggaaggcat cgtcggcgac cagccgtgcg
     2461 aaggcatcgt cgctgacgaa gtggtagtgc tcgccgtcgc gctcgccggg gcgcggagcg
     2521 cgcgtcgtgt gcgacaccga gacctcgacc tgcgcatcct gcgtcagcag cgcgcgcacg
     2581 aggctggtct tgccggcgcc cgagggcgcc gaaacgatgt agagctggcc ggatgcggtc
     2641 atcgcgggga ctccgcgcgg ggcttgccgc cctgcgctca ttcgatcatt cgatcattcg
     2701 atgttctgga tctgctcgcg catctgctcg atcagcacct tcagctccac ggcggcgcgc
     2761 gtggtctcgg tatcgagcga tttcgacgac agcgtgttgg cctcgcggtt gagctcctgc
     2821 atcaggaagt cgagccgccg gccgaccggc tcgggccggc gcagcacgtc gcggatctcg
     2881 gcgaggtggg catcgagccg gtcgagttcc tcctcgacat ccatgcgctg cgcgacgatg
     2941 accatctcct gttcgagccg gcccggatcg gcgccggccg gcaggtcggc gaggcgcccg
     3001 gtgagcttct cgcgcagccg cgcgaccacc tcgggccggc gctggcgcac gaacgcgacc
     3061 tgggcggcga tcgcatcgca gcggctgagg atcatctcgg cggtgcgcgc gccctcgcgc
     3121 tcgcgggtcg cgaccagctc ggcgagcgcg gcgtcgagca cctccagcac gccgcgcttc
     3181 agcggttcga gatcctgctc ggcctcggtg accacgcccg gccagcgcag cacgtcggcg
     3241 aggcgaagcc cggtgccggg accgatcagg ttttcgagct cgccggcaat gccgagcagg
//
LOCUS       DAOQJH010000289         3295 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_18924_length_3295_cov_5.234568, whole genome shotgun sequence.
ACCESSION   DAOQJH010000289 DAOQJH010000000
VERSION     DAOQJH010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3295)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3295)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3295
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18924_length_3295_cov_5.234568"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..1186
                     /locus_tag="PLQ18_07510"
     CDS             2..1186
                     /locus_tag="PLQ18_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006835 - dicarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HNE01536.1"
                     /translation="MIFCTVVVGIAGMEDMKKIGRVGGKALLYFELVTTAALAIGLLI
                     VNLAQPGAGINADPATIDTKAIATYTTAAQKSQTTVEFLMGVIPTNVVDALAKGEILQ
                     VLFFAVLFGFALAAMGEKGRPLVKMIDQFAHGMFGVVNIIMKVAPIGAFGAMAFTIGK
                     YGFHTLGNLALLMGAFYATCLLFIFGVIGAIARACGFSVWKFIKYIKEELLIVLGTSS
                     SESALPRLMDKLERLGCSKPVVGLVVPTGYSFNLDGTSIYLTMAAIFIAQACNIDLTL
                     TQELTILGVLMLTSKGAAGVTGSGFVTLAATLASIGTIPVAGLALILGIDRFMSEARA
                     LTNLIGNGVATIVIAKWEKELDETALHAHLDGRANVEAEIEADAHGERAPLVTATPAQ
                     AV"
     gene            1456..2469
                     /locus_tag="PLQ18_07515"
     CDS             1456..2469
                     /locus_tag="PLQ18_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HNE01537.1"
                     /translation="MLRITLKTLTLGVALALGGLAHAADPILIKFSHVVAQDTPKGKA
                     AEYFKKLAEERTKGAVKVEVYPNSQLYKDKEEVEALQLGAVQMLAPSLAKFGPLGVKE
                     FEVFDLPYILPNRDVLRRVTDGPVGRGLLDKLETKGIKGLAYWDNGFKIMSANKPIHL
                     PADMKGLKVRIQSSKVLDKQMRELGAIPQVMAFSEVYQALQTGVVDGTENPPSNMYTQ
                     KMHEVQKDATVTRHGYLGYAVIVNKKFWDDLPADIRTTLEGAMKDATKYANDIAEKEN
                     ADALEAIKASGKTTVYTPTDAELKQWRDALLPVHAEVASRVGKDLIDAFYKEAAAAGA
                     PIK"
     gene            2561..3205
                     /locus_tag="PLQ18_07520"
     CDS             2561..3205
                     /locus_tag="PLQ18_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HNE01538.1"
                     /translation="MKFLDHLEEWLIAFLIAGATLIVFVSVVHRYLAGLAIPGLQDWL
                     LSLDFSWAQELCIFMFIWMAKFGAAYGVRTGIHVGVDVLINRLSTPMRAKFVLFGLLA
                     GATFTGIVGTFGAGLVAHVMHTGSTTEVLEWPAWILYMAIPLGSYLMCFRFLQVAWAF
                     HRTGDLPHHDHGHVEGVDDPDKPEDINWFALDDDLHPREMSHPIRKPEDGGKPQ"
BASE COUNT          567 a         1155 c         1041 g          532 t
ORIGIN      
        1 gatcatcttc tgcaccgtcg tcgtcggcat cgccggcatg gaagacatga agaagatcgg
       61 ccgcgtcggc ggcaaggcgc tgctgtactt cgagctggtg accaccgccg cgctggcgat
      121 cggcctgctg atcgtcaacc tcgcgcagcc gggtgccggc atcaacgccg atccggcgac
      181 gatcgacacc aaggcgatcg cgacctacac gacggccgcg cagaagtcgc agaccacggt
      241 cgagttcctg atgggcgtga tcccgaccaa cgtcgtcgac gcgctggcca agggcgagat
      301 cctgcaggtg ctgttcttcg cggtgctgtt cggcttcgcg ctggcggcga tgggcgagaa
      361 gggccggccg ctggtgaaga tgatcgacca gttcgcgcac ggcatgttcg gcgtcgtcaa
      421 catcatcatg aaggtcgccc cgatcggtgc cttcggtgcg atggccttca cgatcggcaa
      481 gtacggcttc cacaccctcg gcaacctggc gctgctgatg ggcgcgttct acgcgacctg
      541 cctgctgttc atcttcggcg tcatcggtgc catcgcccgc gcctgcggct tctcggtctg
      601 gaagttcatc aagtacatca aggaagaact gctgatcgtg ctcggcacct cgtcctcgga
      661 atcggcgctg ccgcggctga tggacaagct tgaacgcctc ggctgctcga agccggtggt
      721 cggtctcgtg gtcccgaccg gctactcgtt caacctcgac ggcacctcga tctacctgac
      781 catggcggcg atcttcatcg cccaggcctg caacatcgac ctgacgctga cgcaggagct
      841 gaccatcctc ggcgtgctga tgctgacctc caagggcgcg gccggcgtca ccggctcggg
      901 cttcgtcacg ctggcggcga cgctcgcctc gatcggcacg atcccggtcg ccggtctggc
      961 gctgatcctc ggcatcgacc gcttcatgtc cgaggcgcgc gcgctgacca acctgatcgg
     1021 caacggcgtg gcgaccatcg tcatcgccaa gtgggagaag gaactcgacg agacggcgct
     1081 gcacgcccac ctcgacggcc gcgccaacgt cgaggccgag atcgaggccg acgcccacgg
     1141 cgagcgtgca ccgctggtca cggcgacgcc ggcgcaggcc gtctgaagcc cgacccgccc
     1201 ggacccttcg cggtccgggc aacccagcgg accggtccac gaccggtccg tgccgtgtcc
     1261 ggctcaccgg catttgccgc cggtcagccg gtattaccgc gggcagcgtc ctaagctgtc
     1321 cggcgcacgg gccgccatcg ggcagcggcc cctgcgtacc cgccccgtca ccccccgggc
     1381 ggccggggca tcactccaag cccgaaacgc gatcgttccg aagattccaa ttattagaga
     1441 cgaggagaaa aacccatgct gcgcatcacg ctcaagaccc tgaccctcgg cgtcgccctg
     1501 gcgctcggcg ggctcgccca cgcggccgat ccgatcctca tcaagttctc ccacgtcgtc
     1561 gcccaggaca cgccgaaggg caaggcggcc gaatacttca agaagctggc cgaggagcgc
     1621 accaaggggg cggtcaaggt cgaggtctac ccgaacagcc agctctacaa ggacaaggag
     1681 gaggtcgagg cgctgcagct cggcgccgtg cagatgctcg cgccttcgct cgcgaagttc
     1741 gggccgctcg gcgtcaagga attcgaggtc ttcgacctgc cctacatcct gccgaaccgc
     1801 gacgtgctgc gccgcgtcac cgacgggccg gtcgggcgcg gcctgctcga caagctggag
     1861 accaagggca tcaagggcct ggcctactgg gacaacggct tcaagatcat gtcggcgaac
     1921 aagccgatcc atctgccggc cgacatgaag ggcctgaagg tgcgcatcca gtcctcgaag
     1981 gtgctggaca agcagatgcg tgagctcggc gcgatcccgc aggtgatggc cttctccgag
     2041 gtctaccagg cgctgcagac cggcgtcgtc gacggcaccg agaacccgcc gtcgaacatg
     2101 tacacgcaga agatgcacga ggtgcagaag gacgccacgg tcacgcgcca cggctatctc
     2161 ggctacgcgg tcatcgtcaa caagaagttc tgggacgacc tgccggccga catccgcacg
     2221 acgctcgaag gcgcgatgaa ggacgccacc aagtacgcga acgacatcgc cgagaaggaa
     2281 aacgccgacg cgctcgaagc gatcaaggcc tccggcaaga ccaccgtcta cacgccgacc
     2341 gatgccgagc tgaagcagtg gcgtgatgca ctgctgccgg tgcatgccga ggtcgcctcg
     2401 cgcgtcggca aggacctgat cgacgccttc tacaaggaag ccgctgccgc cggcgcgccg
     2461 atcaagtaag ccgcccccgc gtccgcacct cgccggcccg gcggccctgc cgccgccggc
     2521 cggcgccccg cccggcccgt tgtgcgcgga ggaccccccc atgaaattcc tcgatcacct
     2581 ggaggaatgg ctgatcgcct tcctcatcgc gggtgcgacg ctgatcgtgt tcgtcagcgt
     2641 cgtgcaccgc tacctcgccg gcctggccat tcccggcctg caggactggc tgctgtcgct
     2701 cgacttctcg tgggcgcagg agctctgcat cttcatgttc atctggatgg ccaagttcgg
     2761 cgccgcctac ggcgtgcgca ccggcatcca cgtcggcgtc gacgtgctga tcaaccggct
     2821 gtcgacgccg atgcgcgcga agttcgtgct gttcgggctg ctcgccggcg cgaccttcac
     2881 cggcattgtc ggcaccttcg gcgccgggct ggtcgcgcac gtgatgcaca ccggcagcac
     2941 gactgaggtg ctcgaatggc cggcgtggat cctgtacatg gcgatcccgc tcggctccta
     3001 cctgatgtgc ttccgcttcc tgcaggtggc ctgggccttc caccgcaccg gcgatctgcc
     3061 gcaccacgac cacggccacg tcgagggcgt cgacgatccc gacaagcccg aagacatcaa
     3121 ctggttcgca ctcgacgacg acctgcaccc gcgcgagatg tcgcacccga tccgcaagcc
     3181 tgaagacgga ggcaagccgc aatgaatgcc ctgatcatct tcggactgct gttcggactg
     3241 atgctgaccg gcatgccgat ctcgatttcg ctcggcctga cggtgctgac cttcc
//
LOCUS       DAOQJH010000290         3281 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_19042_length_3281_cov_3.885307, whole genome shotgun sequence.
ACCESSION   DAOQJH010000290 DAOQJH010000000
VERSION     DAOQJH010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3281)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3281)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3281
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19042_length_3281_cov_3.885307"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..420)
                     /locus_tag="PLQ18_07525"
     CDS             complement(<1..420)
                     /locus_tag="PLQ18_07525"
                     /inference="COORDINATES: protein motif:HMM:NF024758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HNE01539.1"
                     /translation="MDERDLGPVARIATAGGRYVGVGLHVRPGGHVLSCAHVVADALG
                     CPDAQFRDEPPAGPVRVNFPFGPAPAAGYTACVADGGWQRNRRPDEAEEPSGRVDIAV
                     LRLDAPAPGVPCADFTADGWRWTGDGDAWTWGLPDGSD"
     gene            complement(442..807)
                     /locus_tag="PLQ18_07530"
     CDS             complement(442..807)
                     /locus_tag="PLQ18_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005159345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CU044_2847 family protein"
                     /protein_id="HNE01540.1"
                     /translation="MRTGDTKIVVFTLASGESVPAEVEDDLVGLSFRPVSRAPDPGEL
                     PRSVQEALARLQPLADAVLDTLSGLTARPDQVEGQFGIKLSAAAGAVIAKAGTEANFA
                     VKLNWKRAEPVPRKRYGKR"
     gene            complement(904..1206)
                     /locus_tag="PLQ18_07535"
     CDS             complement(904..1206)
                     /locus_tag="PLQ18_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009028767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE01541.1"
                     /translation="MRTALVSLALTTLLAGVPLADAVAWTRHGSSSGPHGSSSVDASG
                     SCADRSCSRSVTRTGPAGRTSTHQGEAGCADGSCSGSSTTTGPRGATRTRSGSLSR"
     gene            1517..2308
                     /locus_tag="PLQ18_07540"
     CDS             1517..2308
                     /locus_tag="PLQ18_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014746899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HNE01542.1"
                     /translation="MNDTTGAARSAPPRVGLFVTCLADTIRPSVAFATVKLLEDAGCR
                     VEAPASQTCCGQPAFNSGDRADARALAEQTIAAFEGFDYVVGPSGSCLGMLRHYPELF
                     ETGSPWHARAERLAAKCFEITSFLVDVLGVERVQARYDGTVTYHDSCSGLRELGVKAQ
                     PRKLLATVEGLTLTEMNENEVCCGFGGTFCIKYPDISNRMVTEKVANIRASGAGTLLA
                     GDLGCLMNMAGKLKRQGSDVRVWHVAEVLAGMTDGAPIADAAPSR"
     gene            2327..>3281
                     /locus_tag="PLQ18_07545"
     CDS             2327..>3281
                     /locus_tag="PLQ18_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019619992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HNE01543.1"
                     /translation="MQITSPRFKQNAHEALGDAALQRALGNLKAGFPGKRAAAVARMP
                     EFEQLRDAARDLKNHILEHLDFYLETFEANVKRRGGHVHWARDAEEARAIVLDICRKA
                     GAKTVTKGKSMITEEIGLNDHLAANGIEPIETDLGEYIIQLRGEHPSHIIAPAIHVNL
                     DQVSDAFHAHHGKYGKTERQTEPRALMNEAREILRDKFIAADVGITGANFCIAETGTT
                     VIVTNEGNGDLTQTLPRVHVAVTSIEKVVPTLEDATLLHRLLSRSATGQEIEVYGTWS
                     SGPRRPEDPDGPAEYHVVLLDNGRSNMLGNEFREMLRCIRCS"
BASE COUNT          507 a         1180 c         1118 g          476 t
ORIGIN      
        1 atcgctgccg tccggcagtc cccaggtcca ggcgtcgccg tcgccggtcc accgccagcc
       61 gtcggccgtg aagtccgcac acggcacgcc cggtgccggt gcgtcgaggc gcagcacggc
      121 gatgtcgacc cgtccgctgg gttcctcggc ttcgtccggc cggcggttgc gctgccagcc
      181 gccgtcggcg acgcacgccg tgtagccggc ggccggcgcg gggccgaacg gaaagttcac
      241 gcggaccggt ccggcgggcg gctcgtcgcg aaactgcgcg tcgggacagc ccagcgcatc
      301 ggccacgacg tgcgcgcagc tcagcacgtg gccgccgggc cgcacgtgca ggcccacgcc
      361 gacgtagcgc ccgccggccg tggcgatgcg agcgaccggc ccgagatccc tttcgtccac
      421 aggggcgtcc ctggcgtgga ttcagcgctt gccgtagcgc ttgcgcggca ccggttcggc
      481 ccgcttccag ttcagcttca ccgcgaagtt cgcttcggtg ccggccttgg cgatgaccgc
      541 gccggcagca gcactcaact tgatcccgaa ctgcccctcg acctgatcgg gtcgagccgt
      601 gagcccgctc agcgtatcaa gcacggcatc ggcaagtggc tgcagccggg cgagggcttc
      661 ctgcacgctg cgcggcagct cgccggggtc cggcgcgcgg ctgaccggcc ggaagctcag
      721 gccgaccaga tcatcttcga cctcggccgg cacgctttcg ccggaagcca gcgtgaacac
      781 cacaattttc gtgtcgccag tgcgcattgt cggaacctcc ccgggcggcc tgccgcggtg
      841 agtcaggcac gcgtgcagcg gtttcgcaag gcgtggacgg ccgggtgtcg agtcggcaag
      901 ggttcagcga ctgagcgacc ccgagcgcgt gcgcgtcgct ccgcgcgggc cggtcgtcgt
      961 gctgctgccg ctgcagctgc catcggcgca gcccgcctcg ccctgatgcg tggacgtccg
     1021 cccggccggg ccggtgcgcg tgacgctgcg cgagcagctg cggtccgcgc agctgccgga
     1081 ggcatcgacg ctgctgctgc cgtgcgggcc gctgctgctg ccgtggcggg tccacgcgac
     1141 ggcgtcggcg agcggcacgc cggcgagcag ggtggtgagg gcgaggctga cgagggcggt
     1201 gcgcatggcg ggctcccggg atgcaggcag ggaagggaca ggccgctccc atcctgccct
     1261 gacggtgtga cggctgcgtc gtgcgcaggt cacgactggt atcgggctgc cggtgccgct
     1321 tcgaccggac acctccggct gccgcccggc cggtgcgacc cggcgactac gctttccgca
     1381 tcccggcggc cggttttcac ggcgggttgc ccgggcagct cgtggtcaac aaaaaaacca
     1441 tgcgaactca tgactttggg ccagttctgg tacaatgatg gcgctggtaa aatggtctta
     1501 ccacttagga gagaagatga acgataccac tggcgccgcc cgcagcgccc cgccgcgcgt
     1561 cggcctgttc gtgacctgtc tggccgatac gatccggccg tcggtcgcgt ttgcgactgt
     1621 caagctgctc gaagacgccg gctgccgggt cgaggcaccg gcgagccaga cctgctgcgg
     1681 ccagccggcc ttcaactccg gggatcgcgc cgatgcccgc gccctcgccg agcagaccat
     1741 cgccgcgttc gagggcttcg actacgtcgt cgggccgtcg ggctcgtgcc tcggcatgct
     1801 gcgccactat ccggagctgt tcgagaccgg cagcccctgg catgcgcgcg ccgaaaggct
     1861 ggccgcgaag tgcttcgaga tcacgtcctt cctcgtcgac gtgctcggcg tcgagcgcgt
     1921 gcaggcgcgt tacgacggca ccgtcaccta ccacgattcc tgctccggcc tgcgcgaact
     1981 cggcgtcaag gcgcagccgc gcaagctgct cgccacggtc gagggcctga cgctcaccga
     2041 gatgaacgag aacgaggtct gctgcggctt cggcggcacc ttctgcatca agtatccgga
     2101 catctccaac cgcatggtca ccgagaaggt cgccaacatc cgcgccagcg gcgccggcac
     2161 gctgctcgcc ggcgatctcg gctgcctgat gaacatggcc ggaaagctca agcgccaggg
     2221 ctcggacgtg cgtgtctggc acgtcgccga agtgctggcc ggcatgaccg acggcgcgcc
     2281 cattgcggac gccgccccga gccgctgaag ccccggagcc cgagccatgc agatcaccag
     2341 tccccgtttc aagcagaacg cccacgaggc actcggcgat gccgcgctgc agcgtgccct
     2401 cggcaacctc aaggccggct ttccgggcaa gcgcgccgcc gcggtcgcgc gcatgcccga
     2461 gttcgagcag ctgcgcgatg ccgcgcgcga tctgaagaac cacatcctcg aacacctcga
     2521 cttctacctc gaaaccttcg aggccaacgt caagcgccgc ggcggtcacg tgcactgggc
     2581 gcgcgatgcc gaggaagcgc gcgcgatcgt gctcgacatc tgccgcaagg ccggggcgaa
     2641 gacggtcacc aagggcaagt cgatgatcac cgaggagatc ggcctcaacg atcacctcgc
     2701 ggccaacggc atcgagccga tcgagaccga tctcggcgag tacatcatcc agctgcgcgg
     2761 cgagcacccg agccacatca tcgcgccggc gatccacgtc aacctcgacc aggtttccga
     2821 cgccttccac gcccatcacg gcaagtacgg caagaccgag cgccagaccg agccgcgggc
     2881 gctgatgaac gaggcgcgcg agatcctgcg cgacaagttc atcgccgccg acgtcggcat
     2941 caccggggcg aacttctgca tcgccgagac cggcacgacg gtcatcgtca ccaacgaggg
     3001 caacggcgac ctgacgcaga cgctgccgcg cgtgcacgtg gccgtcacca gcatcgagaa
     3061 ggtcgtgccg acgctggaag acgccacgct gctgcaccgg ctgctgtcgc gctcggcgac
     3121 cgggcaggag atcgaggtct acggcacctg gtcctccggt ccgcgccggc ccgaagaccc
     3181 ggacggccct gccgagtacc acgtcgtgct gctcgacaac ggtcgctcga acatgctcgg
     3241 caacgagttc cgcgagatgc tgcgctgcat ccgctgctcg g
//
LOCUS       DAOQJH010000291         3281 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_19044_length_3281_cov_3.393366, whole genome shotgun sequence.
ACCESSION   DAOQJH010000291 DAOQJH010000000
VERSION     DAOQJH010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3281)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3281)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3281
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19044_length_3281_cov_3.393366"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..580
                     /locus_tag="PLQ18_07550"
     CDS             <1..580
                     /locus_tag="PLQ18_07550"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNE01544.1"
                     /translation="DDDVYGFLVPERPPALASVAPERSCYVTCTSKALMPGLRIGYVA
                     APSAWAERISAAVRTSVWMAPPLMAEIAARWIAEGTAERLMDWQREEIRARQQLAAGR
                     LAAHPYRAQPGGFHLWLPLPEPWREGEFVAAARARGVAVAPAEVFAVGRQPTPPAVRV
                     CVSAQPDRESLAQGLDVLAGLLAQPPSAPLLM"
     gene            677..1399
                     /locus_tag="PLQ18_07555"
     CDS             677..1399
                     /locus_tag="PLQ18_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008946318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein"
                     /protein_id="HNE01545.1"
                     /translation="MTEALFRDDAYLADCTATVTAVTRTGTGTTVECDRTVFYALGGG
                     QPGDTGTLTRADGRRLAILDTRKGETPGTIQHWLAADTEPPAVGERVHLALDWTRRHR
                     HMRMHTALHLLSAVIPAGVTGGSVSADKGRLDFDLPDQRLDKADIEARLNALIAGDHA
                     VGTRWISDAELDAQPELVKTLSVQPPRGQGRVRLVGIAGIDLQPCGGTHVARTGEIGR
                     ILVTKIEKKSTHNRRVILGFAD"
     gene            1558..3186
                     /locus_tag="PLQ18_07560"
     CDS             1558..3186
                     /locus_tag="PLQ18_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012042385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNE01546.1"
                     /translation="MPDDPHVAPSAAVAEALGVVHAYHARSKHRLERYAAGPETLDWS
                     AQPDPFRTYTGAGVQPLPLGADALASRWQAVHRPGAIPPQPLNADTLGTLLELTLGLA
                     AWKQAGPDRWAVRCNPSSGNLHPTEGYVLAQGLPGLADGLWHYRPREHALERRCAWPA
                     PAVSSPRLYVGFSAVLWRELWKYGERAFRYCQLDAGHALGALAYAAAALGWHLRPVSI
                     GHEALAALLGLDRDADFGKAEREEPELLVEVLAGGAASHTLPVIPPDAVWHGQANVLD
                     HWPMYRWPVVDEVARASRRPAGVSEHASPADAALPPLQPAATEVRAATLIRARRSAQR
                     FVRDAQQDLASFLRLLDALLPRTGLPPWSGLNAPVRVHPLLFVHRVEGLAAGVYVLPR
                     SASAVTTLRSALSEGFTWDQPEGVPAHLPLYRLADGTTAKITRTLSCHQALAADCTFT
                     LALLAEFDATLAAAPWAYRELHHEAGLIAQALYLHAEAEGLRGTGIGCFFDDAVHALL
                     GLADARFQSLYHFTVGRPQPDARIASEPPYPDRR"
BASE COUNT          417 a         1241 c         1152 g          471 t
ORIGIN      
        1 cgatgacgat gtctacggct ttctggtgcc ggagcgcccg ccggcgctcg cgagcgtcgc
       61 gcccgagcgc agctgctacg tgacctgcac gtcgaaggcg ctgatgccgg gcctgcgcat
      121 cggctatgtc gcggcgccgt cggcctgggc cgagcgcatc agtgcggccg tgcgcacctc
      181 ggtatggatg gcaccgccgc tgatggccga gattgccgcg cgctggatcg ccgagggcac
      241 ggccgagcgc ctgatggact ggcagcgcga ggagatccgc gcgcgccagc agctcgcggc
      301 cgggcggctg gccgcgcatc cgtaccgcgc gcagcccggg ggcttccacc tgtggctgcc
      361 gctgccggag ccgtggcgcg agggcgagtt cgtcgccgcg gcgcgcgcgc gcggggttgc
      421 cgtggcgccg gccgaggtgt tcgcggtcgg gcgtcagccg acaccgccgg cggtgcgcgt
      481 ctgcgtcagc gcccagcccg atcgcgaatc gctggcgcag gggctggacg tgctcgccgg
      541 cctgctggcg cagccgccgt cggcaccgct gctgatgtga cgacacggca tcggcccgga
      601 cttcgacgca tcctgccggt gtttcgctcg atcccgcccg tcccttgacg attcttgcct
      661 gccggagcct gccgccatga ccgaagcgct gtttcgcgac gacgcctacc tcgccgactg
      721 cacggcgacc gtcaccgccg tgacgcgcac cggcacgggg acgacggtcg aatgcgatcg
      781 caccgtgttc tacgcgctcg gcggcggtca gcccggggat accggcacgc tgacgcgggc
      841 cgacggccgc cggctggcga tcctcgatac gcgcaagggc gaaacccccg gcacgatcca
      901 gcactggctc gccgccgata ccgagccgcc cgccgtcggc gagcgcgtgc atctggcgct
      961 cgactggacg cgccggcacc ggcacatgcg catgcacacg gcgctgcatc tgctgtcggc
     1021 ggtgatcccg gccggcgtca ccggcggctc ggtcagcgcc gacaagggcc ggctcgactt
     1081 cgatctgccc gatcagcggc tcgacaaggc cgacatcgag gcgcgcctga acgcactgat
     1141 cgccggtgat cacgcggtcg gcacgcgctg gatcagcgat gccgaactcg atgcgcagcc
     1201 ggagctggtg aagacgctgt cggtgcagcc gccgcgcggt cagggccgcg tgcgcctggt
     1261 cgggatcgcc ggcatcgacc tgcagccctg cggcggcacg cacgtcgcgc gcaccggcga
     1321 gatcggccgc atcctggtca cgaagatcga gaagaaatcc acgcacaacc gccgcgtgat
     1381 cctcggcttc gcggactgac accgcgcccg cggctgtcgg caagcgcacg ccgcgtcggc
     1441 tttgtcggat atccgtgcat gccggcagga ttccatccgc atgatccgca agcgttttgt
     1501 ccggatggcg cactcctcgc atgctgcagc gcacattcct gacggaccgc tgccgccatg
     1561 cccgacgacc cgcacgtcgc cccttccgct gccgtcgccg aagccctggg cgtggtgcac
     1621 gcctatcacg cgcgcagcaa gcaccggctg gagcgctacg ccgccggccc ggagacgctg
     1681 gactggagcg cgcagcccga tccgttccgc acctacacgg gcgccggcgt gcagccgctg
     1741 cccctcggcg ccgatgcgct cgcgagccgc tggcaggccg tgcaccggcc cggcgcgatt
     1801 ccaccgcagc cgctgaacgc cgatacgctc ggcacgctgc tcgaactgac gctcggcctc
     1861 gcggcctgga agcaggccgg accggatcgc tgggcggtgc gctgcaatcc gtcgagcggc
     1921 aacctgcatc cgaccgaggg ctacgtgctc gcgcaaggcc tgccgggcct cgccgacggc
     1981 ctctggcact accggccgcg cgagcacgcg ctggagcggc gctgcgcgtg gccggccccg
     2041 gcggtctcgt cgccacgttt atatgtgggc ttcagcgccg tgctgtggcg cgaactctgg
     2101 aagtacggcg aacgcgcctt ccgctactgc cagctcgatg ccggccacgc gctcggcgcc
     2161 ctcgcctatg ccgcggccgc gctcggctgg cacctgcggc cggtgtcgat cggacacgag
     2221 gcactggcgg cactgctcgg tctcgatcgc gacgccgact tcggcaaggc cgagcgcgag
     2281 gagccggaac tgctggtcga ggtgctggcc ggtggcgctg cgtcgcacac gctgcccgtc
     2341 atcccgccgg acgccgtctg gcacgggcag gccaatgtgc tcgatcactg gccgatgtac
     2401 cgctggccgg tggtcgatga ggtcgcccgc gccagccgcc ggccggccgg cgtgtcggag
     2461 cacgcatcgc cggcggatgc cgcactgccg ccgctgcagc ccgctgcgac cgaggtccgc
     2521 gccgcgacgc tgatccgcgc acggcgcagc gcacagcgct tcgtgcgcga cgcgcagcag
     2581 gatctcgctt ccttcctgcg cctgctcgat gcgctgctgc cgcgcaccgg cctgccgccg
     2641 tggagcgggc tgaatgcgcc ggtgcgcgtg catccgctgc tgttcgtgca ccgcgtcgag
     2701 gggctggcgg ccggcgtcta cgtactgccc cgcagtgcgt ccgccgtgac gacgctgcgc
     2761 tcggcgctgt cggagggttt cacgtgggac cagcccgagg gcgtgccggc gcatctgccg
     2821 ctgtaccggc tcgccgatgg cacgaccgcg aagatcacgc gcacgctgag ctgccatcag
     2881 gcgctcgcgg ccgactgcac gttcacgctg gcgctgctcg ccgagttcga cgccacgctc
     2941 gccgccgccc cctgggcgta tcgcgaactg catcacgagg ccgggctgat cgcgcaggcg
     3001 ctgtacctgc acgccgaggc cgagggcctg cgcggcaccg gcatcggctg cttcttcgat
     3061 gacgccgtgc acgcgctgct cgggctcgcg gatgcgcgct tccagtcgct gtaccacttc
     3121 acggtcggcc ggccgcagcc ggatgcgcgc atcgccagcg agccgccgta tcccgaccgc
     3181 cgctgattcc cctgccccgt ccctttccgc ctggcccgga gcctgccgcc gatgagcgat
     3241 cgccattaca cccgcctgag cccggccgaa gccgaaaccc t
//
LOCUS       DAOQJH010000292         3265 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_19192_length_3265_cov_4.605919, whole genome shotgun sequence.
ACCESSION   DAOQJH010000292 DAOQJH010000000
VERSION     DAOQJH010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3265
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19192_length_3265_cov_4.605919"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..403)
                     /gene="recO"
                     /locus_tag="PLQ18_07565"
     CDS             complement(<1..403)
                     /gene="recO"
                     /locus_tag="PLQ18_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016353497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HNE01547.1"
                     /translation="MSPGLEPAWVLHTRPYRDTSLLLELLTLTRGRVGAVARGARGPR
                     SRSRGWLRPFQPLLVGLAGRGELATLTSAEAAGAAFWLAGERAVAGLYLNELLLKLLA
                     RDDPQPLLLGHYRAALVALAEPADPEPALRVF"
     gene            complement(422..1369)
                     /gene="era"
                     /locus_tag="PLQ18_07570"
     CDS             complement(422..1369)
                     /gene="era"
                     /locus_tag="PLQ18_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004583187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HNE01548.1"
                     /translation="MTEPTEPTATPDSDADAPNTRAGYVALLGRPNVGKSTLLNRLLA
                     FKLSITSPKPQTTRHTILGIQTLADTQIVYVDTPGLHLNGRKAMNRYMNRAAGNVLGY
                     VDAVVFVIEALRWTDEDAAVLERLADYAGPVLLAVNKVDRVTDKPKLLPFLQQMAEKR
                     AFAAIVPLSAQKGTNAAALETEIAKHLPANPFFFDEDQITTASKRFLAAELVREKLTR
                     VLSDELPYALTVEIEKFEVEGRLHRISAVIWVERKGQKAIVIGDKGGTLKEAGRLARL
                     DMEEMFDAKVHLETWVKVREGWSDDEKALRSLGFDESED"
     gene            complement(1427..2119)
                     /gene="rnc"
                     /locus_tag="PLQ18_07575"
     CDS             complement(1427..2119)
                     /gene="rnc"
                     /locus_tag="PLQ18_07575"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012729076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HNE01549.1"
                     /translation="MHTGLPSAAARLEPALGHRFRDPALRDAALTHRSAGGSNNERLE
                     FLGDALLGCVIGEALYQRLPRAPEGDLTRLRALLVREATLAELARELALGDALALGGS
                     ELKSGGYRRASILADAFEALIGAIYLDSDFETCRRVVLALFGPRLDALPSPSELKDPK
                     TRLQEYLQARQQPLPAYRIVEVSGEAHAQTFTVECTCSGLPAVVASGTSRRKAEQEAA
                     GRMLATLTAGGH"
     gene            complement(2133..2552)
                     /locus_tag="PLQ18_07580"
     CDS             complement(2133..2552)
                     /locus_tag="PLQ18_07580"
                     /inference="COORDINATES: protein motif:HMM:NF027463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4845 domain-containing protein"
                     /protein_id="HNE01550.1"
                     /translation="MRRQQPRPCARALRAGQRGMSPITVLLICGVIGFCMLMVFRIAP
                     IYIADATVSSAMKGIEQEANLARMSAYEIRNRLQRHFDVNDIDAIKSSDVKVTKDAGE
                     LVISIEYETRRPLIYNLDVVAHFTHEQRVKIASASGL"
     gene            complement(2549..>3265)
                     /gene="lepB"
                     /locus_tag="PLQ18_07585"
     CDS             complement(2549..>3265)
                     /gene="lepB"
                     /locus_tag="PLQ18_07585"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HNE01551.1"
                     /translation="LDVWKLAPARAAAGGVGGPRPMPWYVDWSRSFFPVIVVVLLLRS
                     FIVEPFRIPSGSMLPTLRAGDFILVSKFSWGLRLPVSNTEILSTGEVKRGDVAVFRYP
                     EDPSTAFIKRIVGLPGDRLVYRDKQLFVNGQAVEQTKLPGPVSDLEPGFEARQEQLGD
                     VKHLIQVAPRMLDSGAREYVVPPGHYFAMGDNRDNSRDSRYWGYLPAENLIGKAFMIW
                     MNWDCVTGNGHCGRIGRRIE"
BASE COUNT          480 a         1109 c         1158 g          518 t
ORIGIN      
        1 cgaacacgcg cagcgccggt tccgggtcgg caggttcggc gagcgccacc agcgcggcgc
       61 ggtaatggcc gagcagcagc ggctgcggat cgtcgcgggc gagcagcttc agcagcagtt
      121 cgttcaggta caggccggcg acggcgcgct cgccggcgag ccagaaggcg gcaccggcgg
      181 cctcggcgct cgtgagggtc gccagttcgc cgcgcccggc aagcccgacc agcagcggct
      241 ggaacggccg cagccagccg cggctgcgcg aacgcggtcc gcgcgcgcca cgcgccaccg
      301 cgccgacccg gccgcgcgtc agcgtcagca gttcgagcag caggctggtg tcgcggtacg
      361 gtcgcgtgtg cagcacccag gcgggttcga gtccggggga catcacgcca gccgcccggc
      421 ttcagtcctc cgactcgtcg aagccgaggc tgcgcagggc tttttcgtcg tcggaccagc
      481 cttcgcgcac cttgacccag gtctccaggt gcaccttggc gtcgaacatc tcttccatgt
      541 cgaggcgcgc cagccgcccg gcttccttga gcgtgccgcc cttgtcgccg atgacgatgg
      601 ccttctggcc cttgcgctcg acccagatca ccgccgagat ccggtgcagc cggccctcga
      661 cctcgaactt ctcgatctcg acggtcagcg catacggcag ctcgtcggag agcacgcgcg
      721 tgagcttctc gcgcaccagc tcggcggcga ggaagcgctt gctggcggtg gtgatctggt
      781 cctcgtcgaa gaagaacgga ttggccggca agtgcttcgc gatctcggtt tccagcgccg
      841 cggcgttcgt gcccttctgg gccgacagcg gcacgatcgc cgcgaacgcg cgcttttcgg
      901 ccatctgctg caggaagggc agcagcttcg gcttgtcggt gacgcgatcg accttgttga
      961 cggcgagcag caccgggccg gcgtagtcgg cgaggcgttc gagcacggcg gcatcctcgt
     1021 cggtccagcg cagcgcctcg atcacgaaca ccacggcatc gacgtagccg agcacgttgc
     1081 cggcggcgcg gttcatgtag cggttcatcg ccttgcgccc gttcaggtgc aggcccggcg
     1141 tatccacata aacgatctgc gtgtcggcga gcgtctggat gccgaggatg gtgtggcgcg
     1201 tggtctgcgg cttcggcgag gtgatgctga gcttgaaggc gagcaggcgg ttcagcagcg
     1261 tcgacttgcc gacgttgggc cggccgagca gcgcgacgta gccggcacgg gtgttcgggg
     1321 catcggcgtc gctgtccggc gttgcggtgg gttcggtcgg ttcggtcatg gtcacggcat
     1381 ccggacgggc gccgccggca gggcggcgcg acagggaaag gaatgttcag tggccgccag
     1441 cggtgagcgt cgccagcatg cggccggcgg cttcctgttc ggccttgcgc cggctcgtgc
     1501 cgctggcaac gacggccggc aggccgctgc aggtgcattc gaccgtgaag gtctgcgcgt
     1561 gcgcctcgcc gctgacctcg acgatgcggt aggccggcag cggctgctgg cgggcctgca
     1621 ggtattcctg cagccgcgtc ttcgggtcct tgagttcgga cggcgatggc agcgcatcga
     1681 gccgcggacc gaacagcgcc agcacgacgc ggcggcaggt ttcgaagtcg ctgtcgaggt
     1741 agatcgcgcc gatcagcgcc tcgaaggcat cggcgaggat cgaggcgcgc cggtagccgc
     1801 cgctcttcag ttcgctgccg ccgagggcga gcgcatcgcc gagcgccagc tcgcgcgcca
     1861 gttcggcgag ggtcgcctcg cgcaccagca gcgcgcgcag ccgcgtcagg tcgccttcgg
     1921 gcgcacgcgg caggcgctgg tacagcgcct cgccgatcac gcagccgagc agcgcatcgc
     1981 cgaggaattc gaggcgctcg ttgttgctgc caccggccga gcggtgcgtc agcgccgcgt
     2041 cgcgcagcgc cgggtcgcgg aagcggtggc cgagcgccgg ctcaagacgc gcagccgcgc
     2101 tcggcaggcc ggtgtgcatg ccggcgcccg cctcagaggc cggaggcaga ggcgatcttg
     2161 acacgctgtt catgcgtgaa atgcgcaacc acgtcgaggt tgtagatcag cggccggcgg
     2221 gtctcgtact cgatcgagat caccagttcg ccggcatcct tggtgacctt gacgtcgctc
     2281 gacttgatcg cgtcgatgtc gttgacgtcg aagtgccgct gcaggcggtt gcggatctcg
     2341 tacgccgaca tccgcgccag attggcttcc tgctcgatgc ccttcatcgc gctgctgacc
     2401 gtcgcgtccg cgatgtagat cggtgcgatc cggaacacca tcagcatgca gaagccgatc
     2461 acgccgcaga tcagcagcac ggtgatcggc gacatgccgc gctggcccgc ccgcagcgcg
     2521 cgggcgcagg gacggggctg ctgccttctc attcgatcct cctgccgatg cggccgcagt
     2581 ggccgttgcc ggtcacgcaa tcccagttca tccagatcat gaacgccttg ccgatcaggt
     2641 tctccgccgg caggtaaccc cagtagcggc tgtcgcggct gttgtcgcgg ttgtcgccca
     2701 tcgcaaaata gtggcccggc ggcacgacgt actcgcgcgc cccgctgtcg agcatgcgcg
     2761 gcgccacctg gatcaggtgc ttcacgtcgc cgagctgttc ctggcgcgcc tcgaaccccg
     2821 gttcgaggtc gctgaccggg ccgggcagct tcgtctgctc gaccgcctgc ccgttcacga
     2881 acagctgctt gtcgcgatag accagcctgt cgccgggcag gccgacgatg cgcttgatga
     2941 aggccgtcga cgggtcttcc ggatagcgga acaccgccac gtcgccgcgc ttcacctctc
     3001 cggtcgacag gatctcggtg ttgctgaccg gcaggcgcag gccccacgag aacttgctga
     3061 ccaggatgaa gtcgccggcg cgcagcgtcg gcagcatcga gccggacggg atgcggaagg
     3121 gctcgacgat gaaggagcgc agcagcagca cgacgacgat gaccgggaag aaggatcgcg
     3181 accagtcgac gtaccacggc atcggccgcg gcccgccaac cccgccggcg gcggcccggg
     3241 ccggtgccag cttccagaca tcgag
//
LOCUS       DAOQJH010000293         3265 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_19196_length_3265_cov_4.037383, whole genome shotgun sequence.
ACCESSION   DAOQJH010000293 DAOQJH010000000
VERSION     DAOQJH010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3265
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19196_length_3265_cov_4.037383"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            108..1454
                     /gene="metC"
                     /locus_tag="PLQ18_07590"
     CDS             108..1454
                     /gene="metC"
                     /locus_tag="PLQ18_07590"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004121 - cystathionine beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HNE01552.1"
                     /translation="MQDDTRLIHHGRAGAGAGYDGLVNPPVCHASTILFPTLDALEGR
                     TPVPMSYGRHGTPTQFALAEALSALHGGAGTVLVPSGLAAAVLAISTFAHVGTHVLIT
                     DSVYGPTRAYCDELARHRGVTVEYFDPLIGAGIEALLRPETALVFLESPGSLTFEIQD
                     VPAIAAVAHARGVHVAIDDTWSAGYFMKPLALGADISVQALTKYVGGHSDLMLGAVIC
                     NAGTLDAVKHQARLTGNASAPDDVSLALRGLRTLAVRLHQHELSGLDLARWLVQQPQV
                     ARVVHPGRPDHPQHDLWLRDFSGACGLFGFELHPCTRTQLAAFLDGLKLFGMGYSWGG
                     FESLIIPAHAPRTVRKWKTAGPWLRVHVGLEEIEDLINDLALGFQRLSAAAPPPGPAA
                     PAAAETAVAAAGSEPAAPGSDGPPDSDSEELPPAEMTEPAGEQPAAGDPPLPPAVA"
     gene            1637..2521
                     /gene="galU"
                     /locus_tag="PLQ18_07595"
     CDS             1637..2521
                     /gene="galU"
                     /locus_tag="PLQ18_07595"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017838728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HNE01553.1"
                     /translation="MKRKIRKAVFPVAGLGTRFLPATKASPKEMLPIVDKPLIQYAVE
                     EAVAAGVDTLVFVIGRTKNAIADHFDKAYELETELETRGKKDLLKVVQSIVPPHVSCV
                     YVRQAEALGLGHAVLCAKPIVGQEPCAVLLADDLIDAPRGGAMAQMAAHFDKYQCSIL
                     GVERVPPEETFKYGIVTPLPFAAHLSNVDSIVEKPKPEEAPSNLAVVGRYILTPRIFE
                     LLEKTPRGAGNEIQLTDAIAALLLEEQVLAYEFEGRRYDCGSKIGYLTATVEYGMKHA
                     ELGAQFRQYLQSMVCSIG"
     gene            complement(2534..2803)
                     /locus_tag="PLQ18_07600"
     CDS             complement(2534..2803)
                     /locus_tag="PLQ18_07600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE01554.1"
                     /translation="MDDGMPSRRSSTGFMMQQRLRGAKNSAWISLTGPDDRPETLRLR
                     MALMLSSAAGRKTDPYQLADASLLQRKNDDAPQGTGRQLFFTDFL"
BASE COUNT          520 a         1150 c         1079 g          516 t
ORIGIN      
        1 gcgggcgcgc agcggggatc ggctgtggcg attatggcaa cttgtgtccg gccgcaggga
       61 gcgggcgtgc taggctcgcc ggcctcgtcg agccgccgga gggcgccatg caggacgata
      121 cccgactgat ccatcacggg cgggccggag ccggggccgg gtatgacgga ctcgtcaatc
      181 ccccggtctg ccacgcctcg accatcctgt tcccgaccct cgatgcgctc gaaggccgca
      241 cgccggtgcc gatgagctac ggccgccacg gcacgccgac gcagttcgcg ctggccgagg
      301 cgctgagcgc gctgcacggc ggcgccggca cggtgctggt gccctcgggc ctcgcggcgg
      361 cggtgctggc aatctcgacc ttcgcgcacg tcggcacgca cgtgctgatc accgacagcg
      421 tctacggtcc gacccgcgcc tactgcgacg aactcgcgcg ccaccggggc gtgacggtcg
      481 agtacttcga tccgctgatc ggggccggca tcgaggcgct gctgaggccg gaaacggcgc
      541 tggtgttcct cgaatccccc ggttcgctga ccttcgagat ccaggacgtg ccggcgatcg
      601 ccgccgttgc ccatgcccgc ggcgtgcatg tcgcgatcga cgatacctgg agcgccggct
      661 acttcatgaa gccgctcgcg ctcggggcgg acatctcggt gcaggcgctg accaagtacg
      721 tcggcggtca ctccgacctg atgctcggcg ccgtgatctg caacgccggg acgctcgatg
      781 ccgtgaagca tcaggcgcgc ctgaccggca acgccagcgc gcccgatgac gtgtcgctgg
      841 cgctgcgcgg cctgcgcacg ctggccgtgc gcctgcacca gcacgagctg agcggtctgg
      901 acctcgcgcg ctggctggtg cagcagccgc aggtcgcgcg cgtcgtgcat ccgggtcgcc
      961 ccgaccatcc gcagcacgat ctctggctgc gcgacttcag cggtgcctgc ggcctgttcg
     1021 gcttcgagct gcatccgtgc acgcgcacgc agctcgcggc cttcctggat ggcctgaagc
     1081 tgttcggcat gggctattcg tggggcggct tcgagagcct gatcattccg gcgcacgcgc
     1141 cgcgcacggt gcgcaagtgg aagacggcgg ggccatggct gcgcgtgcac gtcgggctgg
     1201 aggagatcga ggacctgatc aacgatcttg cgctcggctt ccagcgcctg agcgccgccg
     1261 ccccgcctcc cggacccgct gcgccggcag cggccgaaac cgcagtggct gccgccggca
     1321 gcgagccggc tgcgcccggc agcgatggcc cgccggacag cgacagcgaa gaacttccgc
     1381 cggcggagat gaccgaaccc gcaggcgagc agcctgcagc cggtgatccg ccgttgccgc
     1441 cggcggtggc ctgaaaccgc tgcgtcaatc ttcgtaccat cgcgccggcg ttcgcttgtc
     1501 gggtctgtgg gcggcgcgca gaatgccgcc gctccacgga tcaccgcgat catttcaggc
     1561 cggaacccgc gatgtccggt catgtccagc cctattcagc cctatccagc agaagacaga
     1621 gcgaggaacc ccgaacgtga agcgcaagat tcgcaaggcc gtgttccccg tcgccggcct
     1681 cggcacccgc ttcctgccgg ccaccaaggc cagcccgaag gagatgctgc cgatcgtcga
     1741 caagccgctg atccagtacg cggtcgagga agcggttgcg gccggcgtcg atacgctggt
     1801 gttcgtcatc ggccgcacca agaacgcgat cgccgatcat ttcgacaagg cctacgagct
     1861 cgagaccgaa ctcgaaaccc gcggcaagaa ggatctgctg aaggtcgtgc agagcatcgt
     1921 gccgccgcac gtctcctgcg tgtacgtgcg tcaggccgaa gcgctcggcc tcggtcacgc
     1981 ggtgctctgc gccaagccga tcgtcggtca ggagccgtgc gcggtgctgc tcgccgatga
     2041 cctgatcgac gcaccgcgcg gcggggcgat ggcgcagatg gcggcgcact tcgacaagta
     2101 ccagtgctcg atcctcggcg tcgagcgcgt gccgcccgaa gaaaccttca agtacggcat
     2161 cgtcacgccg ctgccgtttg ccgcgcacct gtcgaacgtc gacagcatcg tcgagaagcc
     2221 gaagcccgag gaggcgccgt cgaacctggc ggtggtcggt cgctacatcc tgacgccgcg
     2281 catcttcgag ctgctcgaaa agaccccgcg cggtgccggc aacgagatcc agctcaccga
     2341 cgcgatcgcg gcgctgctgc tcgaagagca ggtgctggcc tacgaattcg aaggccggcg
     2401 ctatgactgc ggcagcaaga tcggctatct gaccgcgacc gtcgaatacg gcatgaagca
     2461 cgccgaactc ggcgcgcagt tccgccagta tttgcagtcg atggtctgca gcatcggctg
     2521 agcgctgccc ggctcacaaa aaatctgtaa agaaaagctg acgccccgtg ccctgcgggg
     2581 cgtcgtcgtt tttgcgctgc agcagcgaag cgtccgcaag ctgatacgga tcagttttcc
     2641 gtccggcggc cgatgacagc ataagggcca tccgcaaccg gagggtctca gggcggtcat
     2701 ccggccccgt cagactgatc cacgcggagt ttttcgcgcc ccggaggcgc tgctgcatca
     2761 tgaagcctgt tgatgacctg cgcgacggca tcccgtcgtc catcccccgc gagtcgcagc
     2821 ggacgctgcc ggcccgccat tccgaccccc gggtcgcggt gaccgtaaac ctggtttgcc
     2881 gtacacggcc cggctccgga agccgttctt cccgccccga ccgcaggacc ggcccggccc
     2941 tcaccggctg atccgcgctt tggcgcaacc ccctggaggt tttgattcgc tggccgagcc
     3001 tgccggcagg gatatactcg tcccggccga tgctgcaccg cactataaaa atcaaaggtc
     3061 aggcatgtgc agcggcagca ggtccagcgt cgattttccc cacgtgtgtc gggtcccggc
     3121 cgagccgtga ccgtcaccat ccggcccttc cccctggagt cagccatcat gagcaatccc
     3181 ttcagcctgc acggcaagcg cggcctggtc gtcggcatcg ccaacgagag ttcgatcgcc
     3241 tacgccgtcg cccgcaccat ccgcg
//
LOCUS       DAOQJH010000294         3260 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_19245_length_3260_cov_4.952262, whole genome shotgun sequence.
ACCESSION   DAOQJH010000294 DAOQJH010000000
VERSION     DAOQJH010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3260)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3260)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3260
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19245_length_3260_cov_4.952262"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..389
                     /locus_tag="PLQ18_07605"
     CDS             <1..389
                     /locus_tag="PLQ18_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013699525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE01555.1"
                     /translation="RGRLRVDYNGERREDALAADTGDPFALMLALGDARRSGRPPPNF
                     WQTPDREGLRRYSVVRGTDETLATPLGPLAAERYDCIRVPRAGEDTLRIVVWLAPSLD
                     WLPVQGTRLRNGSEKARFRLAAWARE"
     gene            complement(433..1002)
                     /gene="dcd"
                     /locus_tag="PLQ18_07610"
     CDS             complement(433..1002)
                     /gene="dcd"
                     /locus_tag="PLQ18_07610"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006398615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HNE01556.1"
                     /translation="MSIKSDHWIRRMAREARMIEPFEPGQVRADADGARIVSYGTSSY
                     GYDVRCADEFKVFTNVHSTVVDPKAFDARSFVEVQGDRCIIPPNSFALARTVEYFRIP
                     RDVLVICLGKSTYARCGIIVNVTPLEPEWEGHVTLEFSNTTPLPAVIYANEGVAQMLF
                     IGGDEVCGTSYADRGGKYQGQTGVTLPRT"
     gene            complement(1228..2319)
                     /gene="apbC"
                     /locus_tag="PLQ18_07615"
     CDS             complement(1228..2319)
                     /gene="apbC"
                     /locus_tag="PLQ18_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019025206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HNE01557.1"
                     /translation="MSSLTRPMVDAAIQAYVDPYLDQDLIAAKCLRDVRIDGGSVAVA
                     IELGFPSGEYPAKLAGELETRLRALPGVEAATVTVTTKIIAHEVQKGLKPLPGVKNVI
                     AVASGKGGVGKSTTSVNLALALAADGARVGLLDADIYGPSQPRMLGAMQQPESADGKS
                     IEPVIAHGVQSMSIGYLIEEETPMIWRGPMATQALEQLLRDTRWKDLDYLVIDLPPGT
                     GDIQLTLSQKVPVSGAVIVTTPQDIALLDARKGLKMFEKVEVPILGIVENMSIHICSN
                     CGHAEHIFGAGGGERMAAQYDTKFLGSLPLDIRIREQADNGHPTVAVDPDGQVAGLYR
                     HIARRAAARLALSAKDYSHRFPNIVIQKN"
     gene            complement(2445..2675)
                     /locus_tag="PLQ18_07620"
     CDS             complement(2445..2675)
                     /locus_tag="PLQ18_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011880139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNE01558.1"
                     /translation="MPLFDFRCPACEVTFELLVRSSTVPACPHCGSQTLDRQVARIAP
                     AATTPGLLRNARKVAMKEGHFSNYKPSERPKV"
     gene            2896..>3260
                     /locus_tag="PLQ18_07625"
     CDS             2896..>3260
                     /locus_tag="PLQ18_07625"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017338693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HNE01559.1"
                     /translation="MDMPELLKALILGLVEGATEFLPVSSTGHQIIVADLLDFTGERA
                     KTFEIFIQLGAILAVIWYYRAKIGHVLSTLGSESASRRLIANLLVAFLPAAVLGLAFS
                     KVIKAHLFNPVTVATALIVG"
BASE COUNT          510 a         1121 c         1100 g          529 t
ORIGIN      
        1 cgcgcgggcg gctgcgtgtc gattacaacg gcgagcggcg cgaggacgcg ctcgcggccg
       61 acaccgggga cccgttcgcg ctgatgctcg cgctcggtga cgcccgccgc agcggccggc
      121 cgccgccgaa cttctggcag acgccggatc gcgagggcct gcgccgctac agcgtcgtgc
      181 gcggcaccga cgagacgctg gccacgccgc tcggcccgct cgccgccgaa cgctacgact
      241 gcatccgcgt gccgcgcgca ggcgaggaca cgctgcgcat cgtcgtgtgg ctcgcgccct
      301 cgctcgactg gctgccggtg cagggcacgc ggcttcgcaa cggcagcgag aaggcgcgct
      361 tccggctggc agcctgggcg cgggagtgag gccgggcctg caagcgtctc cccgcccctc
      421 ttcaccttcc gctcaggtgc gcggcagcgt cacgccggtc tggccctggt acttgccgcc
      481 gcgatcggca tacgaagtgc cgcagacctc gtcgccgccg atgaacagca tctgcgcgac
      541 gccctcgttc gcgtagatca ccgccggcag cggcgtcgtg ttcgagaact ccagcgtcac
      601 gtggccttcc cactccggct cgagcggcgt cacgttgacg atgatgccgc agcgcgcgta
      661 ggtgctcttg ccgaggcaga tcaccagcac gtcgcgcggg atgcggaagt actcgaccgt
      721 gcgcgccagc gcgaaggagt tcggcgggat gatgcagcga tcgccctgca cctcgacgaa
      781 gctgcgcgca tcgaaggcct tcgggtcgac gaccgtcgaa tgcacgttgg tgaacacctt
      841 gaattcgtcg gcgcagcgca cgtcgtagcc gtagctcgac gtgccgtagg agacgatgcg
      901 cgcaccgtcg gcatcggcgc gcacctgacc gggctcgaac ggctcgatca tgcgcgcctc
      961 acgggccatg cgccggatcc agtggtcgga cttgatgctc acggacaaac ctcggctcgg
     1021 ccgcaggcgg gccggatcag atgaaggaag ggcgcgcgat tctaaccgcg cgcccttctt
     1081 tataccgatc accgcatacc gatcaccgct gaacacccga tcgtttcagc ccctcccccc
     1141 tcgcggggga ggggttgggg agagggggag aaaaaccatg cggttacgtg tgatcggtat
     1201 tacatgcgcc tgatgcgccg tccgccgtca gttcttctgg atgacgatgt tcgggaagcg
     1261 gtgcgagtag tccttcgccg acagcgccag ccgcgccgcg gcccggcggg cgatgtggcg
     1321 gtagaggccg gcgacctgtc cgtccggatc gacggcgacg gtcgggtggc cgttgtcggc
     1381 ctgctcgcgg atgcggatgt cgagcggcag gctgccgagg aacttggtgt cgtactgcgc
     1441 ggccatgcgc tcgccgccgc cggcaccgaa gatgtgttcg gcgtgaccgc agttcgagca
     1501 gatgtggatg ctcatgttct cgacgatgcc gaggatcggc acctcgacct tctcgaacat
     1561 cttcaggccc ttgcgcgcat cgagcagcgc gatgtcctgc ggcgtcgtga cgatcacggc
     1621 acccgagacc ggcaccttct gcgacagcgt cagctggatg tcgccggtgc ccggcggcag
     1681 gtcgatgacc agatagtcga gatccttcca gcgcgtgtcg cgcagcagct gttcgagcgc
     1741 ctgcgtggcc atcgggccgc gccagatcat cggcgtctct tcctcgatca gatagccgat
     1801 cgacatgctc tgcacgccat gcgcgatgac cggctcgatc gacttgccgt cggccgactc
     1861 gggctgctgc atcgcgccga gcatgcgcgg ctgcgacgga ccgtagatgt cggcatcgag
     1921 caggccgacg cgcgcaccgt cagcggcgag cgccagcgcc agattgaccg aggtcgtcga
     1981 cttgccgaca ccgcccttgc cggaggcgac ggcgatgacg ttcttgacgc cgggcagggg
     2041 cttgagaccc ttctgcacct cgtgcgcgat gatcttcgtc gtgaccgtga ccgtggcggc
     2101 ctcgacgccg ggcagcgcac gcagccgggt ttcgagttcg ccggcgagct tcgccgggta
     2161 ctcgccgctc gggaagccga gttcgatcgc gacggccacg ctgccaccgt cgatgcgcac
     2221 gtcgcgcagg cacttggcgg cgatcagatc ctgatcgagg taggggtcca cataggcctg
     2281 gatggcggca tccaccatcg gccgggtcag gctgctcatc acgactactc cgggcggttt
     2341 cagaaacgac ttgcgcagcc ggtgcggctg cggcgggatg cgggattgta tccgacgtat
     2401 ccgaccgctg cgctcggaaa acccggcttg catccgccgc aggctcagac cttcggacgc
     2461 tccgacggct tgtagttgct gaagtgccct tccttcatcg ccaccttgcg cgcgttgcgc
     2521 agcagccccg gcgtcgtggc cgccggtgcg atgcgggcga cctgcctgtc cagcgtctgg
     2581 ctgccgcagt gcggacaggc cggcacggtg ctcgaacgca ccagcagttc gaaggtgacc
     2641 tcacaggccg ggcagcgaaa atcgaacagc ggcatgaaat cctcccttgt cgcgaacccg
     2701 acagaagtga caaagcaacg gggtgtgcac aggatggatg caagccggat gccgatcacg
     2761 ccctgctcgc cgccgccgcg ccgccgcgcc gccgcgccgc cggcaggatt cacggcgcga
     2821 caagtccggc gaggcggcgc atccccgtag aatgcgcgcc gtgtcccacc cgactgccac
     2881 gaacgaggaa ctgccttgga catgccggaa ctgctgaaag ccctgatcct gggcctggtc
     2941 gaaggcgcga ccgaattcct gccggtgtcg agcaccggcc atcagatcat cgtcgccgac
     3001 ctgctggact tcaccggcga acgtgccaag accttcgaga tcttcatcca gctcggcgcg
     3061 atcctcgcgg tgatctggta ctaccgcgcg aagatcggcc acgtgctctc gacgctcggc
     3121 agcgaatcgg catcccggcg cctgatcgcc aacctgctcg tcgccttcct gccggccgcc
     3181 gtgctcgggc tggcgttcag caaggtgatc aaggcgcacc tgttcaaccc ggtcacggtc
     3241 gccacggcgc tgatcgtcgg
//
LOCUS       DAOQJH010000295         3256 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_19281_length_3256_cov_4.193689, whole genome shotgun sequence.
ACCESSION   DAOQJH010000295 DAOQJH010000000
VERSION     DAOQJH010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3256
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19281_length_3256_cov_4.193689"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..473
                     /locus_tag="PLQ18_07630"
     CDS             <1..473
                     /locus_tag="PLQ18_07630"
                     /inference="COORDINATES: protein motif:HMM:NF018866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1415 domain-containing protein"
                     /protein_id="HNE01560.1"
                     /translation="RFAVCDASQPAVIAERLAGELALLAAADPAIVQTTLLILPNAFA
                     DFLDFNDFLDVADALVEEAGLAGVLQVASFHPGYQFADEAPDDVSHWTNRSPHPVLHL
                     IREDDIERAAATHPDVDGIPDANIAKLRTLGRPAIEALLERCRAAGEAARKPAE"
     gene            470..2062
                     /locus_tag="PLQ18_07635"
     CDS             470..2062
                     /locus_tag="PLQ18_07635"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNE01561.1"
                     /translation="MMSPVAAPAAGRDAFWRDAALLVLALLAVHALNLAVHRDLWVQD
                     EARYGEVLREMLESGRWLVPHLNGFPYPDKPPVYFWLVAALGALTGQGELAFRLFTLL
                     STVAATFGTWVLGRRLAGPAAGRWAALCFVTLFGTLVVGQIARMDMTLCATCVWAWVA
                     LHAHETRRSRAALVGFWLASAAGVAIKGPIALLFTVLPALLRPLGARDWAGFAALKPL
                     HGLGLLVGGVLAWIAAVWLDGESAYLVTIWNEQLVGRAVNSWSHREPVWFYVTLLPVL
                     LMPWTGLAVSGARTFWQSEPIAARFVLACLLVALLAISAISGKLFIYLQPLLPLCAVL
                     AGVGCTTLRAQSRIGTEQALLPVVWFTLLAGGIGWGTHDVLGNNPRGFALATILGLCA
                     ILGFRLSRQPPRRWLIGWTGLSAVVSWVVFGALITLVNPLYSGKPLGEAIARLAPGSR
                     PVAIVHSTRGILNYYAGRLMTEVERTDIAAWYLLHPDALVVIQSAHLSYWFPEGKVPA
                     CTTDETYSVELKEYRVLAGCPR"
     gene            2043..2780
                     /locus_tag="PLQ18_07640"
     CDS             2043..2780
                     /locus_tag="PLQ18_07640"
                     /inference="COORDINATES: protein motif:HMM:NF025736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HNE01562.1"
                     /translation="MPAARADEGIARALLRAQPQRFAGQPLTATMFANVRARAHATLA
                     LAFALLALAALLTALIGDAPLSRHLSSRFWVAGQRSLLKWLTDAGLYAGYACFIALLV
                     LGWWRRQRLWFEVARVWLAAQLIGGLLVVRVLKMWIAHPRPDTGAGDAPLQWLGLTWN
                     AAYNSFPSGHSADIATSAFCLCLLLARPWQRAYVLGFAALIGLTRILLAKHWASDVLA
                     GSAVGLTVSALIAGFWLRRLATGDAPA"
     gene            2883..3218
                     /locus_tag="PLQ18_07645"
     CDS             2883..3218
                     /locus_tag="PLQ18_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013596560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE01563.1"
                     /translation="MASRRIRAALLLAAAVAAPLTAAVAGEFVPVRRVVEEVIVTEAP
                     PPPREEVIIERPSPRHVWVAGHWQRVGNEWVWQNGHWLQPPRLGAGWVPGHWDQRPRG
                     WVWVPGHWA"
BASE COUNT          368 a         1198 c         1150 g          540 t
ORIGIN      
        1 tccgctttgc agtctgcgac gcctcgcagc cggcggtcat cgccgaacgg ctggccggcg
       61 aactcgcgct gctcgccgcg gccgacccgg cgatcgtgca gaccacgctg ctgatcctgc
      121 cgaacgcctt cgcggacttc ctggacttca acgacttcct cgatgtcgcc gatgcactgg
      181 tcgaggaagc cgggctcgcc ggcgtgctgc aggtggcctc gttccatccc ggataccagt
      241 tcgccgacga ggcccccgac gatgtctcgc actggaccaa ccgctcgccg catccggtgc
      301 tgcacctgat ccgcgaggac gacatcgagc gcgccgccgc cacgcatccg gatgtcgacg
      361 gcatcccgga cgccaacatc gcgaagctgc gcacgctcgg acgcccggcg atcgaggcgc
      421 tgctcgaacg ctgccgggcc gccggcgaag ccgcccggaa gcctgccgaa tgatgtcacc
      481 ggtcgccgcc cccgctgccg gccgggatgc cttctggcgc gatgccgcgc tgctcgtgct
      541 cgccctgctc gccgtgcatg cgctgaacct cgccgtgcat cgcgatctct gggtgcagga
      601 cgaggcccgc tacggcgagg tgctgcgcga gatgctcgaa tccgggcgct ggctggtgcc
      661 gcacctgaac ggcttcccgt atccggacaa gccgccggtg tacttctggc tggtcgccgc
      721 actcggcgcg ctgaccggcc agggcgaact ggcgttccgg ctgttcacgc tgctgtcgac
      781 cgtcgccgcg accttcggca cctgggtgct cgggcgacgc ctcgccggtc cggcggccgg
      841 gcgctgggca gcactctgct tcgtgacgct gttcggcacg ctggtggtcg gccagatcgc
      901 gcgcatggac atgacgctct gcgcgacctg cgtctgggcc tgggtggcgc tgcacgcgca
      961 tgagacccgc cgctcgcgcg cggcgctcgt cggcttctgg ctcgcgagtg ccgccggcgt
     1021 cgcgatcaag ggtccgatcg cgctgctgtt cacggtgctg ccggcgctgc tgcgccccct
     1081 cggcgcgcgc gactgggcgg gcttcgccgc gctgaagccg ctgcacggcc tcggcctgct
     1141 ggtcggcggc gtgctcgcgt ggatcgcggc ggtctggctc gacggcgaga gcgcgtatct
     1201 cgtgaccatc tggaacgagc aactggtcgg ccgggccgtg aattcctggt cgcaccggga
     1261 gccggtctgg ttctacgtga cgctgctgcc ggtgctgctg atgccatgga ccgggctggc
     1321 ggtcagcggc gcgcgcacgt tctggcagag cgaaccgatc gcggcgcgct tcgtcctcgc
     1381 ctgcctgctg gtcgcgctgc tcgcgatctc ggcgatctcc gggaagctgt tcatctacct
     1441 gcagccgctg ctgccgctgt gcgcggtgct cgccggcgtc ggctgcacga cgctgcgcgc
     1501 gcagtcacgc atcggcaccg aacaggcgct gctgccggtg gtgtggttca cgctgctcgc
     1561 cggcggcatc ggctggggca cgcacgacgt gctcggcaac aacccgcgcg gcttcgcgct
     1621 ggccacgatc ctcggcctgt gcgcgatcct cggcttccgg ctgtcgcgcc agccgccgcg
     1681 gcgctggctg atcggctgga ccggactgtc ggccgtggtg tcctgggtgg tgttcggcgc
     1741 gctgatcacg ctcgtcaacc cgctgtactc cggcaagccg ctcggcgagg ccatcgcccg
     1801 gctggcgccc ggctcgcggc cggtggcgat cgtgcattcg acgcgcggca tcctgaacta
     1861 ctacgccggc cggctgatga ccgaggtcga gcgcaccgac atcgccgcgt ggtatctgct
     1921 gcatcccgat gcgctggtcg tgatccagtc cgcgcacctg tcgtactggt tcccggaggg
     1981 caaggtgccg gcctgcacga ccgacgagac ctactcggtc gagctgaagg aataccgtgt
     2041 ccttgccggc tgcccgcgct gacgagggca tcgctcgcgc gctgctgcgc gcgcagccgc
     2101 agcgcttcgc cgggcagccg ctgacggcga cgatgttcgc gaacgtgcgc gcccgcgccc
     2161 acgccacgct cgcgctcgcc ttcgcgctgc tcgcgctggc ggcgctgctg acggcactca
     2221 tcggcgatgc gccgctcagc cggcacctct ccagccgctt ctgggtcgcc ggacagcgca
     2281 gcctgctgaa gtggctgacc gacgccggcc tgtacgccgg ctatgcgtgc ttcatcgccc
     2341 tgctcgtgct cggctggtgg cggcgtcagc ggctgtggtt cgaagtcgcg cgcgtatggc
     2401 tcgccgcgca actcatcggc ggcctgctcg tcgtgcgcgt gctgaagatg tggatcgcac
     2461 acccgcgccc cgacaccggc gccggcgatg cgccgctgca gtggctgggc ctgacctgga
     2521 atgccgccta caacagcttc ccgtccgggc actcggccga catcgccacc agcgccttct
     2581 gcctctgcct gctgctggcg cgcccgtggc agcgcgccta cgtgctcggc ttcgcggcgc
     2641 tgatcgggct gacgcgcatc ctgctcgcca agcactgggc cagcgacgtg ctggccggca
     2701 gcgcggtcgg actgacggta tcggcgctga tcgccggttt ctggctgcgc cggctcgcca
     2761 ccggcgacgc cccggcttaa gcctcgccta agcgatccgg cgcgaacgcg gcgcggcgcg
     2821 ccggctcgaa gcgggcgtgc cggacgtttc cggtgtcatc cgtcctcggg aggaccgcca
     2881 tcatggcatc ccgtcgaatc cgggcagccc tgctgctcgc cgctgccgtc gcggcaccgc
     2941 tcaccgcggc cgtggccggg gagttcgtgc cggtacggcg cgtggtcgag gaagtcatcg
     3001 tcaccgaagc gccgccgccg ccgcgcgagg aagtcatcat cgagcgcccg tcgccgcgcc
     3061 acgtctgggt cgccggccac tggcagcggg tcggcaacga gtgggtctgg cagaacggcc
     3121 actggctgca gccgccgcgc ctcggcgccg gctgggtgcc gggtcactgg gatcagcgcc
     3181 cgcgcggctg ggtgtgggtg ccgggacact gggcgtgaac gccgttgccc gttgcgggct
     3241 ctgtctcgcg ctcgcc
//
LOCUS       DAOQJH010000296         3253 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_19305_length_3253_cov_3.683552, whole genome shotgun sequence.
ACCESSION   DAOQJH010000296 DAOQJH010000000
VERSION     DAOQJH010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3253
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19305_length_3253_cov_3.683552"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1183)
                     /locus_tag="PLQ18_07650"
     CDS             complement(<1..1183)
                     /locus_tag="PLQ18_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007672497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNE01564.1"
                     /translation="MAHVIVLGAGAMGLAAAHHLLKAGHTVELFEADRVAGGMAAHFD
                     FGGLSIERFYHFVCKADAPTFALLEELGLAQAMRWRPTKMGYFHDGKLYRWGDPLALL
                     AFPKLDPVSKLRYAWQMFMATKRTDWRDLDGISAAEWLRRGSGERAWKVLWEKLFTLK
                     FFELSEQVSAAWLWTRIKRVGTSRRSLMQEELGYIDGGSETLVKALVASIERQGGRLH
                     LGTPVREVTLEGGAVTGIVTAGGERVAAGHVISTVPMPLIPKLIPALPEALKARYAAL
                     RNIGAVCVIHKLRRAVTGNFWLNTNDARIEVPGLVEFSNLRPFADGTHIVYVPYYMPQ
                     THPKFARDDDFFVRESFGYLKLVNPQLTDADRLDSAVGRLQYAQPLCPPDSRCTWPKA
                     TG"
     gene            complement(1223..2164)
                     /locus_tag="PLQ18_07655"
     CDS             complement(1223..2164)
                     /locus_tag="PLQ18_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003484138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNE01565.1"
                     /translation="MDKNDKIVVLGAAGLVGQNLIVELKAQGYRQLVAIDKHAHNLGV
                     LAQLHPDVTTVLADLAEPGPWREHLRGAARVLQLQAQITGKEAQLFVRNNLDSTKLVL
                     EAVREHQVPFLVHISSSVVNSVADDDYTNTKKAQEKLVLDSGLPCCVLRPTLMFGWFD
                     PKHLGWLSRFMAKVPVFPIPGHGRYMRQPLYNRDFCRIIVQCMERAPDRAIYDIVGDT
                     RIDYVDIIHTIRRVKGLRTPIVHIPYTLFRWLLLGYALFSDKPPFTADQLKALTAGDD
                     FTGVDTEAEFGIRQMPFEDAIRESYCDPRYAHIVLER"
     gene            complement(2209..2946)
                     /locus_tag="PLQ18_07660"
     CDS             complement(2209..2946)
                     /locus_tag="PLQ18_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010961784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNE01566.1"
                     /translation="MRKVLIVGATSAIAEACARRFAAEGDALCLCARRAGRLDVLAAD
                     LRVRGAALVAVEVLDANDVAGHAAMLDRATAALGGLDTVLIAHGTLSDQAACEASVES
                     TLAEIHTNGLSTVALLTELAGRFERQRHGTLAVISSVAGDRGRQSNYVYGSAKALVTA
                     FTGGLRQRLSKAGVNVLTIKPGFVDTPMTAAFPKGPLWASVDTIAAGIVRAIERRSSV
                     VYLPGFWRPIMAIIRAIPEPVFRRIRL"
     gene            complement(2996..>3253)
                     /locus_tag="PLQ18_07665"
     CDS             complement(2996..>3253)
                     /locus_tag="PLQ18_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018177042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HNE01567.1"
                     /translation="ASPGWLSFPRPGYTLALDFPNRGAATFALLDRLDAVVREAAGAI
                     YPAKDARMSGALFRAAYPRWQRFADYLDPQFSSSFWRRVMA"
BASE COUNT          521 a         1070 c         1152 g          510 t
ORIGIN      
        1 ctccagttgc tttcggccag gtgcacctgg aatccggcgg gcagagcggc tgcgcgtact
       61 gcaggcgccc gaccgccgaa tcgaggcggt cggcgtcggt gagttgcgga ttgacgagct
      121 tcaggtagcc gaaggattcg cgcacgaaga agtcgtcgtc gcgcgcgaac ttcggatggg
      181 tctgcggcat gtagtacggc acgtagacga tgtgcgtgcc gtcggcgaag ggccgcaggt
      241 tcgagaattc gaccagaccc ggcacctcga tgcgcgcatc gttggtgttg agccagaagt
      301 tgccggtgac cgctcggcgc agcttgtgga tcacgcacac ggcgccgatg ttgcgaagcg
      361 ccgcgtagcg ggccttgagg gcttcgggca gcgccgggat cagcttcggg atcagcggca
      421 tcggcacggt gctgatgacg tgaccggccg cgacgcgctc gccgccggcc gtgacgatgc
      481 cggtcaccgc accgccttcg agcgtgacct cgcgcaccgg cgtgccgagg tgcaggcgcc
      541 cgccctggcg ctcgatgctc gcgaccagcg ccttcaccag cgtctcggag ccgccgtcga
      601 tgtagccgag ttcctcctgc atcaggctcc tgcgcgaggt gccgacgcgc ttgatgcgcg
      661 tccacagcca cgcggccgag acctgctccg acagctcgaa gaacttcagc gtgaagagct
      721 tctcccagag caccttccag gcgcgctcgc cgctgccgcg acgcagccac tcggcggcgc
      781 tgatgccgtc gagatcgcgc cagtccgtgc gcttcgtggc catgaacatc tgccacgcgt
      841 agcgcagctt cgagaccggg tcgagcttcg ggaaggcgag cagcgccagc gggtcgcccc
      901 agcggtacag cttgccgtcg tggaaatagc ccatcttcgt cggccgccag cgcatggcct
      961 gagcgaggcc gagttcttcg agcagcgcaa aggtcggtgc gtcggccttg cagacgaagt
     1021 gatagaagcg ctcgatgctc aggccgccga agtcgaaatg cgcggccatg ccgccggcga
     1081 cccggtcggc ttcgaagagc tcgaccgtgt gaccggcctt cagcaggtga tgcgcggcgg
     1141 cgaggcccat ggcgccggcg ccgagcacga tgacgtgtgc catgaggaca ggattctccg
     1201 ggtagggggc gagtgcgggc gctcagcgtt cgagcacgat gtgcgcgtag cgcggatcgc
     1261 agtagctttc gcggatcgcg tcctcgaacg gcatctgccg gatgccgaat tcggcttcgg
     1321 tgtcgacgcc ggtgaaatcg tcgccggcgg tcagcgcctt cagctggtcg gccgtgaacg
     1381 gcggcttgtc gctgaacagc gcatagccga gcagcagcca gcggaacagc gtgtacggga
     1441 tgtgcacgat cggcgtcctc aggcccttga cgcgcctgat cgtgtggatg atgtcgacgt
     1501 agtcgatgcg cgtgtcgccg acgatgtcgt aaatcgcccg gtccggcgcg cgctccatgc
     1561 actggacgat gatcctgcag aagtcgcggt tatagagcgg ctggcgcatg tagcggccgt
     1621 ggccggggat cgggaacacc ggcaccttcg ccatgaagcg cgacagccag ccgaggtgct
     1681 tcgggtcgaa ccagccgaac atcagcgtcg ggcgcagcac gcagcacggc aggccgctgt
     1741 cgagcacgag cttttcctgc gccttcttgg tgttggtgta gtcgtcatcg gcgaccgagt
     1801 tgaccaccga cgaactgatg tgcacgagaa acggcacctg atgctcgcgc acggcttcga
     1861 gcacgagctt cgtcgaatcc aggttgttgc gcacgaacag ctgcgcttcc ttgccggtga
     1921 tctgcgcctg cagctgcagc acgcgcgccg cgccgcgcag gtgttcgcgc cacgggccgg
     1981 gctcggcgag gtcggccagc accgtggtca cgtccggatg cagctgcgcc agcacgccga
     2041 ggttgtgcgc gtgcttgtcg atcgccacca gttgccggta gccctgcgcc ttcagctcga
     2101 cgatcaggtt ctggccgacg aggccggcgg cgccgagtac gacgatcttg tcgttcttgt
     2161 ccatcgggat cggttccggt cgggaatgca ggaaagggga gcttcggcct acaggcggat
     2221 gcgccggaac accggctcgg ggatcgcgcg gatgatcgcc atgatcggcc gccagaagcc
     2281 gggcaggtag acgacgctgc tgcgccgctc gatcgcccgg acgatgccgg cggcgatcgt
     2341 atcgacgctc gcccacagcg gccctttcgg gaaggcggcg gtcatcggcg tatcgacgaa
     2401 gccgggcttg atggtcagca cgttgacgcc ggccttcgag agccgctggc gcaggccgcc
     2461 ggtgaaggcc gtgaccagcg ccttggcgct gccatagaca taattgctct gccggccgcg
     2521 atccccggcc accgacgaga tcaccgccag cgtgccgtgg cgctggcgct cgaagcgccc
     2581 ggcaagctcg gtcagcagcg cgacggtgct caagccattg gtgtggatct cggcgagcgt
     2641 cgattcgacc gaggcctcgc aggccgcctg gtcggacagc gtgccgtgcg cgatcagcac
     2701 ggtgtcgagc ccgccgagcg cggccgtcgc acgatcgagc atcgctgcgt gcccggcgac
     2761 atcgttggca tcgagcacct caactgcgac caatgctgcc ccgcgcacgc gcaggtcggc
     2821 cgccagcacg tcgagccgcc cggcgcgccg tgcgcacagg cagagcgcgt cgccttcggc
     2881 ggcgaagcgg cgggcacagg cttcggcaat cgccgaggtc gcgcccacga tcaggacctt
     2941 gcgcatcatc ggctcccgga tgtgccagtc attgcggttg cgtgtccagc catcgtcagg
     3001 ccatgacccg acgccagaag ctcgacgaga actgcgggtc gaggtaatcg gcgaaacgct
     3061 gccagcgcgg atacgcggcc cggaacagcg cgccgctcat gcgcgcgtcc ttggccggat
     3121 agatcgcgcc ggcggcctcg cggaccaccg catcgagccg gtcgagcagc gcgaaggtgg
     3181 cggcgccgcg gttcgggaaa tccagcgcca gcgtgtagcc ggggcgcggg aacgacagcc
     3241 agcccggcga ggc
//
LOCUS       DAOQJH010000297         3250 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_19343_length_3250_cov_4.504538, whole genome shotgun sequence.
ACCESSION   DAOQJH010000297 DAOQJH010000000
VERSION     DAOQJH010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3250
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19343_length_3250_cov_4.504538"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1408
                     /locus_tag="PLQ18_07670"
     CDS             <1..1408
                     /locus_tag="PLQ18_07670"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HNE01568.1"
                     /translation="LPGDFGLVSMAAPIVMFIELLRSFSFDMALIQKADAEAAHFDTA
                     WTLNLLCTTLIALLMVLAAAPAADFYQEPRFAPVMAWLALGTFIQGFENIGTVNFRKE
                     LDFGQEFRLMLYKKLASITLTALLAWWLRNYWALVWGTLASRVFGVLLSYHMSHYRPS
                     LRLSHWRDLMHFSKWMVVNNLLFFLNLRGVDFVIARLFGAAVLGQYLVAIEISTILSV
                     ELVAAANRAFFPGFVKLSGEPNTVVRHCTTVMAAAAMWLLPLAMLLYLLGDLFVHAMF
                     GDKWPLTGELIGEFGLFGALTAFTGSLSYAYMAVGHPRFSSVAAGVQSLIVLGLLVLN
                     GYWGRPEWIGHCFVLTACLVLFVHAAMVKHVFPALQLLRWLRGVARMSLAAAGAGLVI
                     SSLKAPLLGSGLPEPVVLVGLGIGGVALYGAGVWLLWQLDHCKDGPERSILTRIPRLQ
                     AYIERRRSPYDWIAEGRI"
     gene            1409..2317
                     /locus_tag="PLQ18_07675"
     CDS             1409..2317
                     /locus_tag="PLQ18_07675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE01569.1"
                     /translation="MKLLQQRFAQLRRKWHELQFRRAAAGVRGIGPIRLAAEGPIVLS
                     MVQQRDVDAYLLALGTFVRRLPARRIVVVADPSLDAADQAVLRRQVPDIEIVAQHDFR
                     PEGLPSGGCWERLVAITRYVSEGYVIQLDADTVATGPLDEVAACVRDNRAFTLGTDDH
                     FVLQPASEAADFARSRASPDAHIQVHAEAALDRLPAEVFPAYIRGCAGFAGFPAGYEM
                     LPKLLQLSAVMEQAIGARWRQWGSEQFASNVMVANAPASCVLPHPKYCAPHRRRADSV
                     FLHFIGYVRYIDGTYARLARGYLKTA"
     gene            2365..3204
                     /locus_tag="PLQ18_07680"
     CDS             2365..3204
                     /locus_tag="PLQ18_07680"
                     /inference="COORDINATES: protein motif:HMM:TIGR01444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HNE01570.1"
                     /translation="MSLDLRRFAKLPLYLRAFGPLAGLRLYAAIERAGLPVKSETVQA
                     HAVPGYDAPVSLRRARADHATFWQCLVQRQYDLDRFPQTARLQQTYEAMLARGERPLI
                     IDGGANIGLAALWLARRFPQAQIVSVEPDTGNLALLRRNLAGLGARAQVLDGGLWPRS
                     GRLRIVNPESGSAAFRVEETTGDGPGTLRAYTIDEICTLAGCREPLIVKLDIEGAQQY
                     LFASATEWVGRAHLITLELDDWLFPWRGSSRPFFRVLGEHPFDYLLGGESVFCFRDAG
                     AAV"
BASE COUNT          426 a         1122 c         1137 g          565 t
ORIGIN      
        1 gctgccgggc gacttcggcc tggtgtcgat ggctgcgccg atcgtgatgt tcatcgagct
       61 gctgcgctcg ttcagcttcg acatggcgct gatccagaag gccgatgccg aagccgcaca
      121 tttcgatacc gcctggacgc tgaacctgct ctgcacgacg ctgatcgcgc tgctgatggt
      181 gcttgccgcc gcgccggcgg cggacttcta tcaggagccg cgctttgcgc cggtgatggc
      241 ctggctcgcg ctcggcacct tcatccaggg cttcgagaac atcggcaccg tcaacttccg
      301 caaggaactc gacttcggcc aggaattccg gctgatgctg tacaagaagc tcgccagcat
      361 cacgctgacg gcgctgctgg cgtggtggct gcgcaattac tgggcgctgg tctggggcac
      421 gctggcgagc cgggtgttcg gcgtgctgct gtcctaccac atgagccact accggccgtc
      481 gctgcgcctg agccactggc gcgacctgat gcacttctcg aagtggatgg tcgtcaacaa
      541 cctgctgttc ttcctgaacc tgcgcggcgt cgatttcgtc atcgcccgcc tgttcggtgc
      601 cgcggtgctc ggccagtacc tggtggcgat cgagatcagc acgatcctgt cggtcgagct
      661 ggtggccgcc gccaaccgtg ccttctttcc gggtttcgtc aagctctccg gcgagccgaa
      721 caccgtcgtg cgccactgca cgacggtgat ggcggcggcg gcgatgtggc tgctgccgct
      781 ggcgatgctg ctgtacctgc tcggcgacct gttcgtgcat gcgatgttcg gcgacaagtg
      841 gccgctgacc ggcgagctga tcggcgagtt cgggctgttc ggtgcgctga cggcgttcac
      901 cggctcgctg tcgtatgcgt acatggccgt cggccacccg cgcttcagct cggtggccgc
      961 cggcgtgcag agcctgatcg tgctggggct gctggtgctg aacggctact gggggcggcc
     1021 ggagtggatc gggcactgct tcgtgctgac cgcctgcctc gtgctgttcg tgcatgcggc
     1081 gatggtcaag cacgtgttcc cggccctgca gctgctgcgc tggctgcgcg gcgtcgcgcg
     1141 catgagcctc gccgccgccg gcgccggtct ggtcatcagc agcctgaagg cgccgctgct
     1201 cggcagcggc ctgccggagc cggtcgtgct ggtcgggctc ggcatcggcg gcgtggcgct
     1261 ctacggcgcc ggcgtctggc tgctctggca gctcgatcac tgcaaggacg gccccgagcg
     1321 cagcatcctg acccggattc cccgcctgca ggcgtacatc gaacgccggc gcagccctta
     1381 tgactggatc gcggaaggtc gcatctgaat gaagctcctg cagcaacgat tcgcgcagct
     1441 ccggcgcaag tggcatgaac tgcagttccg gcgcgccgcg gccggcgtgc gcggcatcgg
     1501 tccgatccgc ctcgccgccg aggggccgat cgtgctgtcg atggtccagc agcgcgatgt
     1561 cgatgcctac ctgctcgccc tcggcacctt cgtgcgccgg ctgccggcgc ggcgcatcgt
     1621 cgtcgtcgcc gatccgagcc tcgatgcggc cgatcaggcc gtgctgcgcc ggcaggtgcc
     1681 ggacatcgag atcgttgcgc agcacgattt ccgccccgag ggcctgccga gcggcggctg
     1741 ctgggagcgg ctggtcgcga tcacgcgcta cgtgagcgag ggctacgtga tccagctcga
     1801 cgccgacacg gtcgcgaccg gcccgctcga cgaggtggcc gcctgcgtgc gcgacaaccg
     1861 cgcgttcacg ctcggtaccg atgaccactt cgtgctgcag ccggcgagcg aggcggcgga
     1921 cttcgcacgc tcgcgtgcca gcccggacgc ccacatccag gtgcacgccg aggctgcgct
     1981 cgaccggctg ccggcggagg tgttcccggc ctacatccgc ggctgcgccg gcttcgccgg
     2041 tttcccggcc ggctacgaga tgctgccgaa gctgctgcag ctgtcggcgg tcatggagca
     2101 ggcgatcggg gcgcgctggc ggcagtgggg ctcggagcag ttcgcgtcga acgtgatggt
     2161 cgccaacgcg ccggcgagct gcgtgctgcc gcacccgaag tactgcgcgc cgcaccggcg
     2221 ccgggccgat tcggtgttcc tgcacttcat cggctacgtg cgctacatcg acggcaccta
     2281 cgcgcggctc gcacgcggct acctgaagac cgcctgaggc ccgcacggcg gcatcacgga
     2341 gagtttctgg ggagagtttc tggcatgagt ctggatcttc ggcgcttcgc gaaactgccc
     2401 ctgtacctgc gggccttcgg gccgctggcg gggctgcgcc tgtatgccgc catcgaacgc
     2461 gccgggctgc cggtgaagtc ggaaaccgtg caggcgcatg cggtgccggg ctacgacgcg
     2521 ccggtgagcc tgcgccgggc ccgcgccgat cacgcgacct tctggcaatg cctggtgcag
     2581 cgccagtacg acctcgaccg cttcccgcag acggcgcgcc tgcagcagac ctacgaggcc
     2641 atgctcgcgc gcggcgagcg gccgctgatc atcgacggcg gcgccaacat cgggctggcg
     2701 gcactgtggc tggcgcggcg tttcccgcag gcgcagatcg tctcggtcga accggacacc
     2761 ggcaatctgg cgctgctcag acgcaacctc gccgggcttg gcgcgcgcgc gcaggtgctc
     2821 gacggcggtc tctggccgcg cagcgggcgg ctgcgcatcg tcaaccccga gtccggctcc
     2881 gcggccttcc gcgtcgagga aaccaccggc gacggccccg gcacgctgcg cgcgtacacg
     2941 atcgacgaga tctgcacgct cgccggctgc cgcgagccgc tgatcgtcaa gctcgacatc
     3001 gagggggcgc agcaatacct gttcgcgagt gcgaccgaat gggtcgggcg cgcgcacctg
     3061 atcacgctcg aactcgacga ctggctgttc ccgtggcgcg gcagcagccg gccgttcttc
     3121 cgggtgctcg gcgagcatcc gttcgactac ctgctcggcg gcgagagcgt gttctgcttc
     3181 cgcgacgccg gggccgcggt gtgaacgccg atgcgtgaca tcgccatcct gctgatcgtc
     3241 acctacggcc
//
LOCUS       DAOQJH010000298         3246 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_19382_length_3246_cov_4.296772, whole genome shotgun sequence.
ACCESSION   DAOQJH010000298 DAOQJH010000000
VERSION     DAOQJH010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3246
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19382_length_3246_cov_4.296772"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(239..1189)
                     /locus_tag="PLQ18_07685"
     CDS             complement(239..1189)
                     /locus_tag="PLQ18_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015672644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase A"
                     /protein_id="HNE01571.1"
                     /translation="MQILFCHPNAAPGEAERWQAGLTARLGAAGLGVPGFTIRTPEVA
                     APPADYALLWNPPPAALLGQTRLRAAFALGAGVDALLGADVLPPGVPLYRLEDAGMAA
                     QMVEYALHAVLRWQRRFDEYEVLAARGEWRTLTPRPRPLIAVLGLGALGGAVASAFAG
                     LGYRVRGWSRSPRALAGVEGFAGPEALMACLDGAGVLIDLLPLTAGTRGLLDGRVFAR
                     LMPGALFVNLARGAHVVEADLLAALAGGRLSAAVLDVFGQEPLPAGHALRDRPEVTVT
                     PHIAALTRLDAALDSIAAAITAIEAGATAVPGRVDPARGY"
     gene            1272..3245
                     /locus_tag="PLQ18_07690"
     CDS             1272..3245
                     /locus_tag="PLQ18_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014618594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HNE01572.1"
                     /translation="MSLTARLKELVVGAPRDPLVAEARRRTALLTVLAWAGLGADGLS
                     SVCYGPEEAFLALGQHTHLGLYLAVATFATVFVIALAYNQVIELFPGGGGGYKVATQL
                     LGTRAGLVSGAALMVDYVLTIAISIAASMDALFSLLPPGWGGAKRYIELTLLLLLVLL
                     NLRGYRETMRVLLPIALGFVLVHGGLLIWGIGAHAGQLPDLIRASVAETGSLTADVGW
                     LMVTGLFLKAYSLGGGTYTGLEAISNNVNRIAEPRVRNGKRTMGLMALTLAMTAGGLI
                     LLYLLWAAEPVAGQTLNAVTFRSLISTWQVGDTLPEQLLLWLVLLLEAGLLLVAANTG
                     FYSGPGMLGNMAADAWVPRHFRSLSARLVTQNGVVLMGAGAFVILLWSGGEVSLLIVL
                     YTINVFLTLSLALAGLVCHWWRERHDDPRWLRRLSLALAACCLTSGILLVTTVEKFAE
                     GGWVTVLITSAVIALCAVVRRHYDAVRDELRKVDALLAPPLGPEEDDEPRPPKVDRSA
                     ATAIFLVGQNRGIAIHAFLWVFRMFPGHFRNCVFLAVGEVDAGAVNGQNALRSLRLRT
                     ENSLHYFVRVCARNDIAAETRAAFGPDPVEELHRLTVEVMDDFPNSVCFASKLIFASD
                     SWLGPMLHNQTALSMQRRMHLDGRQMVIVPLKV"
BASE COUNT          443 a         1202 c         1113 g          488 t
ORIGIN      
        1 cggccctggc gctggagctt ggtcaggcga tacatggcgg atgtccctct catccatccg
       61 tggcaacgcg acacagaata atacgatttg ccggccattc cgtacggcag atggcgtaat
      121 ccgccccgcc tcatgcctgc aggatctgcg atccggaccg gctgcacgca ggcccggccc
      181 ttgcggccgc gagccttggg tcagcgctgc cagccggacg ctgcgccgcc ggtgctgctc
      241 agtaaccgcg ggccggatcg acgcgaccgg gcacggccgt ggcgccagcc tcgatcgccg
      301 tgatggcggc agcgatgctg tcgagagccg cgtcgagccg cgtcagcgcc gcgatgtgcg
      361 gcgtgaccgt gacctccgga cgatcccgca gcgcatgacc ggccggcagc ggctcctgcc
      421 cgaacacatc gagcaccgcc gccgacagcc ggcccccggc gagcgcggcc agcaggtcgg
      481 cctcgaccac gtgcgcgccg cgcgcgaggt tcacgaacag cgcccccggc atcagccgcg
      541 cgaacacccg gccatcgagc aggccgcgcg tgccggcggt cagcggcagc aggtcgatca
      601 gcacgccggc gccatcgagg caggccatca gggcttccgg tcccgcgaag ccctcgacgc
      661 cggccagcgc ccgcggcgaa cggctccagc cgcgcacgcg gtagccgagt ccggcgaagg
      721 cggaagccac cgcgccgccg agtgcgccga ggccgagcac ggcgatcagc ggtcgcggcc
      781 gtggcgtcag cgtccgccat tcgccgcgcg ccgcgagcac ctcgtattcg tcgaagcgcc
      841 gctgccagcg cagcacggcg tgcagtgcgt attcgaccat ctgtgcggcc atgccggcgt
      901 cttcgagccg gtacagcggc acgccgggcg gcagcacatc ggcaccgagc agcgcatcga
      961 cgccggcccc gagcgcgaag gccgcacgca gccgcgtctg cccgaggagt gccgccggcg
     1021 gcggattcca cagcagcgcg taatcggccg gcggcgctgc aacctccggc gtccggatcg
     1081 tgaagccggg cacgcccagg ccggccgcgc cgagccgcgc ggtcagtccc gcctgccagc
     1141 gctcggcctc gccgggggcg gcgttcgggt gacagaacag gatctgcatg acctgcgtcc
     1201 tcgccggcgg ccgggaccgg cttgatagac tggccagtct agggggccga acgcccgcac
     1261 gggatcaccg catgtcgctg acagccagac tcaaggaact cgtcgtcggc gcgccgcgcg
     1321 acccgctggt cgccgaggcg cgccggcgca cggcactgct gaccgtactg gcctgggccg
     1381 gactcggcgc cgacggtctt tcatccgtgt gttacggccc cgaggaagcc ttcctcgcgc
     1441 tcggccagca cacgcacctc ggcctgtacc tcgccgtcgc gaccttcgcg accgtgttcg
     1501 tgatcgcgct cgcctacaac caggtcatcg agctgttccc gggcggcggc ggcggttaca
     1561 aggtcgccac gcaactgctc ggcacgcgcg ccggcctggt gtcgggcgcg gcgctgatgg
     1621 tcgattacgt gctgaccatc gccatctcga tcgccgcgtc gatggatgcg ctgttctcgc
     1681 tgctgccgcc gggctggggc ggcgccaagc gctacatcga gctgacgctg ctgctgctgc
     1741 tggtgctgct gaacctgcgc ggctatcgcg agaccatgcg cgtgctgctg ccgatcgcgc
     1801 tcggcttcgt gctcgtgcat ggcggcctgc tcatctgggg catcggtgcc cacgccggcc
     1861 agctgccgga cctgatccgc gcctcggtcg ccgaaaccgg ctcgctgacc gccgacgtcg
     1921 gctggctgat ggtcaccggc ctgttcctga aggcctattc gctcggcggc ggcacctaca
     1981 ccggcctcga agcgatctcg aacaatgtca accgcatcgc tgaaccgcgc gtgcgcaacg
     2041 gcaagcgcac gatggggctg atggcgctga cgctggcgat gaccgccggc ggcctgatcc
     2101 tgctgtacct gctgtgggcc gccgagccgg tcgccggcca gacgctgaac gccgtgacct
     2161 tccgctcgct gatctcgacc tggcaggtcg gcgacacgct gcccgagcag ctgctgctgt
     2221 ggctggtgct gctgctcgaa gccgggctgc tgctggtggc cgccaacacc ggcttctaca
     2281 gcggccccgg catgctcggc aacatggcgg ccgatgcctg ggtgccgcgg catttccgct
     2341 cgctgtcggc gcggctggtc acgcagaacg gcgtcgtgct gatgggtgcc ggcgccttcg
     2401 tgatcctgct gtggtcgggc ggcgaggtgt cgctgctgat cgtgctgtac acgatcaacg
     2461 tgttcctgac gctgtcgctg gcgctggccg ggctggtctg ccactggtgg cgcgagcgcc
     2521 acgacgaccc gcgctggctg cggcggctgt cgctcgcgct cgccgcctgc tgcctgacct
     2581 ccggcatcct gctggtgacc acggtcgaga agttcgccga gggcggctgg gtgacggtgc
     2641 tgatcaccag cgccgtcatc gcactctgcg cggtcgtgcg ccgccactac gacgccgtgc
     2701 gcgacgaact gcgcaaggtc gatgcgctgc tcgccccgcc gctcggcccc gaggaggacg
     2761 acgaaccgcg cccgccgaag gtcgaccgca gcgccgcgac cgcgatcttc ctggtcggcc
     2821 agaaccgcgg catcgccatc cacgccttcc tgtgggtgtt ccggatgttc ccggggcact
     2881 tccgcaactg cgtgttcctc gccgtcggcg aggtcgacgc cggcgccgtc aacggccaga
     2941 acgcgctgcg ctcgctacgg ctgcgcaccg agaactcgct gcactacttc gtgcgcgtct
     3001 gcgcccgcaa cgacatcgcc gccgaaaccc gcgccgcctt cggccccgac ccggtcgagg
     3061 agctgcaccg gctgaccgtc gaggtcatgg acgacttccc gaacagcgtc tgcttcgcct
     3121 cgaaactgat cttcgcctcc gactcctggc tcggcccgat gctgcacaac cagaccgcgc
     3181 tgtcgatgca gcggcgcatg cacctcgacg gccggcagat ggtcatcgtg ccgctgaagg
     3241 tctgaa
//
LOCUS       DAOQJH010000299         3243 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_19413_length_3243_cov_4.307089, whole genome shotgun sequence.
ACCESSION   DAOQJH010000299 DAOQJH010000000
VERSION     DAOQJH010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3243
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19413_length_3243_cov_4.307089"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..434
                     /locus_tag="PLQ18_07695"
     CDS             <1..434
                     /locus_tag="PLQ18_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018145083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HNE01573.1"
                     /translation="HYTDWTIGHVHSGALGWVALVSFGSFYHLFPRLYDTRIHSTKLI
                     YVHFWLATVGIVLYITSMWVAGIGQGLMLRGFDDYGNLAYSFIETVEFLHKPYVWRAI
                     GGAFFVAGILLMAYNLVMTALDARRERSKLEAKLAAKLART"
     gene            456..1190
                     /gene="ccoO"
                     /locus_tag="PLQ18_07700"
     CDS             456..1190
                     /gene="ccoO"
                     /locus_tag="PLQ18_07700"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017942921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit II"
                     /protein_id="HNE01574.1"
                     /translation="MRHEHIEVNSGLMIALVLAVISIGGLVEIVPLYFINDTVEKVDG
                     VRPYTPLELRGRDIYVREGCFLCHSQMIRPFRDEWLRYGHYSLAAESKYDHPFQWGSK
                     RTGPDLARVGNKFSNQWHVQHLVKPTSVVPESIMPNYPWLLDTELDYTDVADRMKALR
                     YTGVPYSLTQAEYEKNVKDFGKVVADQLDISQAKDNLMKQARTFNYDGLPDRVTEMEA
                     LVAYLQVLGTMVDFSKYDEGYFANFR"
     gene            1262..1483
                     /locus_tag="PLQ18_07705"
     CDS             1262..1483
                     /locus_tag="PLQ18_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002710160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit 3"
                     /protein_id="HNE01575.1"
                     /translation="MFDWLSWFTRLDNSKIFSLLLFFGTFCGIVIYVFGSRRRGRRLE
                     SYKHIPFQDDEPADRHDAPAHKATDGDER"
     gene            1480..2415
                     /gene="ccoP"
                     /locus_tag="PLQ18_07710"
     CDS             1480..2415
                     /gene="ccoP"
                     /locus_tag="PLQ18_07710"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015257063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit III"
                     /protein_id="HNE01576.1"
                     /translation="MSDTTTTTSTPGAVQTTGHAWDGDLQEFNNPLPRWWLWSFYATV
                     VFAVFYWFAYPAWPFGKTWTKGFTTATYTEGGQSHTMRWNTRSALLEDLDSGSDAKRQ
                     RQYLDKIAQTDYAAIAKDPQMVAFVNQAGKRLFETNCSACHGRGGQGVVGLFPNLVDD
                     DWLWGGSYQAIETTLKHGRNGYMPAFGSVLNATQIDDVANYVLSLSGVVPSSEASVRG
                     QKIFTGATGGCYMCHGDDGKGIKSMGAANLTDKIWTIANVPAAQSPADKLAAVRAVIG
                     SGVQNVRKMPAWGERLSAAEIKLMAVYVHQLGGGQ"
     gene            2569..>3243
                     /locus_tag="PLQ18_07715"
     CDS             2569..>3243
                     /locus_tag="PLQ18_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018950977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HNE01577.1"
                     /translation="MAEEAQLYQKRLAIHPRSVEGRFRKLKTGVVLLAYGVYFLLPWL
                     RWSRPTGVNQAVLFDLPGRRFYLFDLVVQAQDIFWLAGLLVIAATLLFFVTGLAGRVW
                     CGYFCFQTLWTDVFIMIEQAIQGERPARLRLAKQPWDAEKRRKMLLTWASWLGVAFLT
                     GLTFTLYWADAPTLVGEFLTGAAPFAAYATTGFLTATTFVMAGLAREQVCTYMCPYAR
                     FQSAMFD"
BASE COUNT          542 a         1096 c         1050 g          555 t
ORIGIN      
        1 cgcactacac cgactggacc atcggccacg tgcactccgg tgcgctcggc tgggtggcgc
       61 tggtcagctt cggttcgttc taccacctgt tcccgcgcct gtacgacacg cgcatccaca
      121 gcaccaagct gatctacgtg cacttctggc tcgcgacggt cggcatcgtg ctgtacatca
      181 cctcgatgtg ggtcgccggc atcggccagg gcctgatgct gcgtggcttc gatgactacg
      241 gcaacctcgc ctacagcttc atcgagaccg tcgagttcct gcacaagccc tacgtgtggc
      301 gcgccatcgg cggtgcgttc ttcgtcgccg gcatcctgct gatggcttac aacctcgtga
      361 tgaccgcgct cgatgcgcgt cgcgagcgca gcaagctcga agccaagctc gcggccaagc
      421 tggcccgcac ctgatccgac tggagtccaa gcgacatgag acacgagcat attgaggtca
      481 actccggcct gatgatcgcg ctggtgctgg ccgtcatcag catcggcggt ctggtcgaga
      541 tcgtgcccct gtacttcatc aacgacaccg tcgagaaggt cgacggcgtg cgcccgtaca
      601 cgccgctcga actgcgcggt cgcgacatct acgtgcgcga gggctgcttc ctgtgccact
      661 cgcagatgat ccggccgttc cgtgacgagt ggctgcgcta cggccactac tcgctggccg
      721 ccgagagcaa gtacgaccac ccgttccagt ggggctcgaa gcgcaccggt ccggatctgg
      781 cgcgcgtcgg caacaagttc tcgaaccagt ggcacgtgca gcatctggtc aagccgacct
      841 cggtcgtgcc cgagtcgatc atgccgaact acccgtggct gctcgacacc gaactcgact
      901 acaccgatgt cgccgatcgc atgaaggcgc tgcgctacac cggcgtgccc tactcgctga
      961 cgcaggccga gtacgaaaag aacgtcaagg acttcggcaa ggtggtggcc gaccagctcg
     1021 acatcagcca ggccaaggac aacctgatga agcaggcgcg caccttcaac tacgacggcc
     1081 tgccggaccg cgtcaccgag atggaggcgc tggtcgccta cctgcaggtg ctcggcacga
     1141 tggtcgactt cagcaagtac gacgaaggct acttcgcgaa cttccgctga gcattcggtg
     1201 attcggcgaa cgggccgcgc tccggcgacg gggcgggccc ggatgattcg gtaggcgcga
     1261 cgtgttcgac tggctgtcct ggttcacccg tctcgacaac agcaagatct tctcgctgct
     1321 gctgttcttc gggaccttct gcggaatcgt gatctacgtc ttcggcagcc gccgccgcgg
     1381 ccgccggctg gagtcgtaca agcacattcc gtttcaggac gacgagccgg ctgaccggca
     1441 tgacgcgccc gcgcacaagg cgacggacgg tgacgaacga tgagtgacac gacgacgaca
     1501 acgagcacgc ccggggcggt gcagaccacc ggtcacgcct gggacggcga cctgcaggag
     1561 ttcaacaatc cgctgccgcg ctggtggctg tggtcgttct acgcgaccgt ggtctttgcg
     1621 gtgttctact ggttcgcgta tccggcctgg cccttcggca agacctggac caagggcttc
     1681 acgaccgcga cctacaccga gggcggccag tcgcacacga tgcgctggaa cacgcgcagc
     1741 gccctgctcg aagacctcga cagcggctcg gatgccaagc gccagcgcca gtacctcgac
     1801 aagatcgcgc agaccgatta cgcggcgatc gccaaggacc cgcagatggt cgcgttcgtg
     1861 aaccaggccg gcaagcgcct gttcgagacc aactgctcgg cctgccatgg ccgcggcggt
     1921 cagggcgtgg tcgggctgtt cccgaacctc gtcgacgacg actggctgtg gggcggcagc
     1981 taccaggcca tcgagacgac gctcaagcac ggccgcaacg gctacatgcc ggccttcggc
     2041 agcgtgctga acgccacgca gatcgacgat gtcgcgaact acgtgctgtc gctgtccggt
     2101 gtcgtgccgt ccagcgaggc cagcgtccgc ggccagaaga tcttcaccgg cgccaccggc
     2161 ggctgctaca tgtgccacgg cgacgacggc aagggcatca agtcgatggg tgcggcgaac
     2221 ctgaccgaca agatctggac gatcgccaac gtcccggctg cccagagccc cgccgacaag
     2281 ctcgcggcgg tcagggcggt gatcggcagc ggcgtgcaga acgtgcgcaa gatgccggcc
     2341 tggggcgagc gcctcagtgc cgccgagatc aagctgatgg ccgtctacgt gcatcagctc
     2401 ggcggcggcc agtaagcaag ccggacgggt tccggcagcg gacgagtgcg aaccgtcagt
     2461 gccctggcgc cgcccggcgg cgatgcttga cggctccgtc ccacctccgg cggcgaccgc
     2521 agtgcctgcg ctgcggtcgc cgtgccgtta cgaccacccg gaaagtccat ggccgaagaa
     2581 gcacagctct accagaagcg tctggcgatc catccccgct ccgtcgaagg gcggttccgc
     2641 aagctcaaga ccggcgtcgt gctgctcgcc tacggtgtct acttcctgct gccgtggctg
     2701 cgctggtcgc ggccgaccgg cgtcaaccag gcggtgctgt tcgacctgcc gggccggcgc
     2761 ttctacctgt tcgatctggt cgtgcaggcg caggacatct tctggctcgc cggcctgctg
     2821 gtgatcgccg cgacgctgct gttcttcgtg accggtctgg ccgggcgcgt ctggtgcggc
     2881 tacttctgct tccagacgct ctggaccgat gtgttcatca tgatcgagca ggcgatccag
     2941 ggcgaacgcc cggcgcgcct gcggctggcg aagcagccct gggatgccga gaagcgccgc
     3001 aagatgctgc tgacctgggc gtcctggctg ggcgtggcct tcctgaccgg cctgaccttc
     3061 acgctgtact gggccgatgc accgacgctg gtcggtgagt tcctgaccgg ggccgcgccg
     3121 tttgcggcct atgcgaccac cggcttcctg accgcgacca ccttcgtgat ggccgggctc
     3181 gcgcgcgagc aggtctgcac ctacatgtgt ccgtacgcgc gcttccagag cgcgatgttc
     3241 gac
//
LOCUS       DAOQJH010000300         3240 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_19434_length_3240_cov_4.765149, whole genome shotgun sequence.
ACCESSION   DAOQJH010000300 DAOQJH010000000
VERSION     DAOQJH010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3240)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3240)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3240
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19434_length_3240_cov_4.765149"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(376..2646)
                     /locus_tag="PLQ18_07720"
     CDS             complement(376..2646)
                     /locus_tag="PLQ18_07720"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNE01578.1"
                     /translation="MTQYELVPSSSSSEGEGNDFVFLSESEPLETLASSANTDPDLWR
                     VLAVDDDPDFQRSMAFALSGMCVLGRKIELLQAGSHAEAAQILAREQDIAVMLLDVVM
                     ETDDAGLRLARAVREVLGNAELRIVLVTGQPGFAPVREVMEHYDINDYWSKSELGIER
                     LHTLLTANTRAWMQVHTIARARRGLQMIVEASNVLVGARNLNDFSARTIAEIASVLGV
                     APEGVVCVRRTRNEVCIIGAAGRYAGTVGKSIDSIATARVHEAISTCLREQRTLIRAD
                     CMVLYFPSSLAGSEYVAWVEIENELDATEQELLRVFAMNIGGGLHNVALFSRLDELAY
                     SDSLLGLPNRNALLRAIEAARSGSARQDHTLLLLDVDSFAGLNLALGPAYGDSLLRRI
                     GQQLRQRLDPTVLIARVGGDLFALLGPSHLIDPAEVRRKLADDLDAVKDGGTCTFGSA
                     ALPLARFEGNAGEALTTAMLLMRNAKRRGHGQHQDWIPSLAEEAGSRFELLNQFRDAL
                     ESDGFTIELQPQVNLTTGAVVGVEALARWRRADGSRVPPDVFIGLAETTGLIQPLSER
                     IYAQAFEAADQLAEAGFAHVHVAINVSARQFMSNGQLPKFQNHLGAKMISAKRIEVEI
                     TESTAMENYSVIRGTLESLRRCGHTVAIDDFGTGYSSLACLRNLPADLLKIDRSFVQE
                     IGGEADQTAIADLVIRLGQRLGLNTIAEGVETPAQAGWLRARGCNLAQGWLFSRPLPL
                     DVLIPWLEKGQGRVQV"
BASE COUNT          671 a         1060 c          914 g          595 t
ORIGIN      
        1 aacagcgtca gttcctcggc caccggcgcg gcgagtgccg gcagcagtgc aaacagcagg
       61 atcaaaaggg cgaggctggg gcgaagccgg ggaatccgca tgattcgcgc tccgggaagg
      121 atcgggcagg ccgcgggtgg cgtggcgccg ggacgcacga cgggggcgaa aaggttcagg
      181 gcacggatgc agggccggct ccgtgcgcat cgcacggatc gaccggagcg gagggcaaag
      241 tccggcagcc gccagcgaag gatgacacgg cgcttcgctc cggatcatgc cctccgggca
      301 cggcggcggt ttggcagacc ccgcagccgc ggcgcattcc ggatccggac ggaaccgggc
      361 tccggggtca acgcatcaga cctggactct gccctgccct ttctccagcc acggaatcaa
      421 tacatcaagc ggcagagggc gggaaaacag ccatccctga gccagattac aaccgcgtgc
      481 ccgcagccag ccggcctgtg ccggcgtctc cactccttcg gcaatcgtat tcaggcccag
      541 tctctgacca agccgaatca ccagatcagc aatggcagtc tgatccgcct cgccaccgat
      601 ttcctgcacg aagctcctgt caatcttcag cagatcggca ggcagattcc gcaggcaggc
      661 cagggaagaa taaccggtgc caaaatcatc tattgccacc gtatgtccgc aacggcgcag
      721 gctctccagc gtcccccgga ttacggaata attttccatc gcggtacttt ccgtgatttc
      781 aacttcgatc cgcttcgcgg aaatcatctt ggcgccgaga tgattctgga atttcggcaa
      841 ctggccattg ctcataaact gtcttgccga tacatttatc gcaacatgaa catgagcaaa
      901 tccggcctcg gcaagttgat cagccgcttc aaacgcctgc gcgtaaatgc gctcgctcaa
      961 aggctgtatc aggcctgtcg tttcagccag gccaataaaa acatccggcg gaacacggct
     1021 gccatccgcc cggcgccagc gggccagcgc ctccaccccc accactgccc cggtggtcag
     1081 gttgacctgc ggttgcagtt caatcgtgaa gccgtcactt tccagcgcgt cgcgaaactg
     1141 gttcagcaac tcgaacctgc tgccggcctc ctcggccagc gagggaatcc agtcctgatg
     1201 ctgcccatgc ccgcgccgct tggcattccg catcagcagc atggcggtcg tcagcgcctc
     1261 gccggcattg ccctcgaacc tggccagcgg caatgctgcg ctgccaaagg tgcaggtacc
     1321 gccgtccttc actgcatcga gatcatccgc gagcttgcga cgcacttccg ccgggtcgat
     1381 caggtggctg gggccaagca gtgcaaacaa gtcaccgccc acccgggcga tcagcactgt
     1441 tggatcaagc ctttgccgca gttgctgacc gatgcgtcgc aacagcgaat ccccatatgc
     1501 aggaccgagt gccagattga gtccggcaaa gctgtcgaca tccagcagca gcaaggtgtg
     1561 atcctgccgc gcactgcctg atcgggctgc ttcgatggct cgcagcagag cattccggtt
     1621 cggaagtccg agcagcgaat cgctataggc cagttcgtca agacgactga acaaggcgac
     1681 attgtgcaga cctccgccaa tgttcatggc gaagacacgc aacagctcct gctcggttgc
     1741 atccagttcg ttctcgatct ccacccaggc cacgtactcg gaaccggcca gggaagaagg
     1801 gaaatacagc accatgcagt ctgcccggat cagcgtgcgc tgctcccgca gacaggtgga
     1861 gatggcctca tgcacgcggg ccgttgcaat cgaatcgatg gatttcccga ccgtgccggc
     1921 atagcggccg gcggccccga tgatgcagac ctcgttccgg gtgcgccgca cacagacgac
     1981 gccctccggt gcgacgccga gcacggaggc aatctcggcg atcgtgcggg ccgagaaatc
     2041 gttcagattc cgtgcaccga ccagtacatt gctggcctcg acgatcatct gcaggccacg
     2101 acgggcacgg gcgatcgtat gcacctgcat ccaggcgcgg gtgttggcgg tcagcagcgt
     2161 gtgcagacgc tcgattccga gctccgactt cgaccagtag tcattgatgt catagtgctc
     2221 catgacctca cgtaccggcg caaaacccgg ctgcccggtc acgagcacga tgcgcagctc
     2281 ggcattcccg agcacttcgc ggaccgcccg ggccagcctg agacccgcgt cgtccgtttc
     2341 catgaccaca tcgagcagca tcaccgcaat gtcctgctcc ctggccagga tttgtgcggc
     2401 ctcggcatgg ctgccggcct gcagcagttc aatcttcctg ccgagcacgc acatgccgga
     2461 aagagcgaag gccatggagc gctggaagtc ggggtcatca tcgacagcca gcacccgcca
     2521 gagatcagga tccgtgttgg cactggaagc cagcgtctcc agcggctctg attctgacag
     2581 gaagacaaaa tcattccctt ccccttccga cgacgatgaa gagggcacca gttcgtattg
     2641 cgtcatgatt gcctcacaat tgcatgcttg tagcgcccat caactccgca ctggcagaat
     2701 gcctgaaagc aattctgatc catcccggac attttccatg aatctggcgt gattcatgcg
     2761 ttcaatcacc tttgatcctt gagatgcagg ttcaatcatt accaaatata tagtattttc
     2821 aatacgacaa ctgcagcagt aatgattcac gatttatagg taagcaatct ggaaatggca
     2881 tttttttgac acttattcag cctgtcattg aaacacctcg caccgtcaac aaatcgtcaa
     2941 caacctgacg gcctcgaacg aattaccgca tctgcccgtt caggacatgg ctttcagcga
     3001 tcttttcagg aagtccgcac gacacgcctg agcgatgccg gcttaccgcc ggccccgtgc
     3061 gcttcattgc aatgaccccg gagagggtgc aggcgctcgg tttcaccggt caccgcacgc
     3121 cggcgcgccg ccgccgggca gcgctcatca gcgcggtgcc gggtgtcacc ccacccgcag
     3181 ccggaacgcc cgctcctcgc cgctgccggc ctcgcgctcg atcatctcga ccctgccatc
//
LOCUS       DAOQJH010000301         3234 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_19499_length_3234_cov_3.542623, whole genome shotgun sequence.
ACCESSION   DAOQJH010000301 DAOQJH010000000
VERSION     DAOQJH010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3234)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3234)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3234
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19499_length_3234_cov_3.542623"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1753)
                     /locus_tag="PLQ18_07725"
     CDS             complement(<1..1753)
                     /locus_tag="PLQ18_07725"
                     /inference="COORDINATES: protein motif:HMM:NF039586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HNE01579.1"
                     /translation="MSMRRIAVRGLLVNAALLAVLLVALALMGEATGNSARFGELYSW
                     LLVGSALGFVLLAFLIGRNLWDLYRQVRNDEPGSRLTLRLFVLFSLISVAPVSVVYYF
                     SVQFIARSIDSWFDVRVEKALEDALELSRTALDLRMREVLKQTVRLAGQLGETPGPPS
                     TLLLAELREESDAQELAVLKADGTPLVVAASEAASLVPERPPDVVLAQLRQGGNYVAL
                     DPTAELGFQLRAVVPVPAHATIGDDLLLQALYPVTDRFNSLGHSVQAAFDAYRELVFL
                     REPLRTTFIMTLSLVLLFGVLSALSAAVYFARKFVQPVRDLADGTRAVAAGQYDKQLA
                     PAANDELGFLVQSFNEMTRNLASARDSALQSRHMLEGQRAYLETVLARLSSGVLTLDH
                     DGHPRTFNAATLHILGLDAGHPLSAEGESPLAHLLGAVAGHLAGESAEWRQEVTLFAP
                     GGRRVLMCRGSRLPDAVGLKGGHVLVFDDITTLVQAERDAAWTEVARRLAHEIKNPLT
                     PIQLSAERMRRRFLGNMSEEDAKVMDRATHTIVQQVEAMKAMVNAFSEYARAPTMSVA
                     PFSLNQLVTEVADLYRAQ"
     gene            complement(1761..2336)
                     /locus_tag="PLQ18_07730"
     CDS             complement(1761..2336)
                     /locus_tag="PLQ18_07730"
                     /inference="COORDINATES: protein motif:HMM:NF025692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HNE01580.1"
                     /translation="MTAFTDRHRTPCLALLLGLLCLWLAPVPAAADGFRVLRASSTSE
                     RGLLRLDALIDLQFTEAPLDALRNGVPLTIEIDVEIVRDRDLLWDETVASLSQRFRLE
                     YHALARQYVAVNLNTNDLRSFPTLAGALDAIGRLHDFPLIDRALLKPPGNYYGRLRAS
                     LDIEALPPPLRPVAYLSTDWRLGSTWYAWSL"
     gene            complement(2333..>3234)
                     /gene="rsmB"
                     /locus_tag="PLQ18_07735"
     CDS             complement(2333..>3234)
                     /gene="rsmB"
                     /locus_tag="PLQ18_07735"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019625971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HNE01581.1"
                     /translation="AAQPVAVHAHPAWLLERLQADWPADWPAIVEASNQQAPMMLRVN
                     LARESRDEALARLATAGIAAAAHAQVASAVVLEHPAEVSALPGFADGRLSVQDGAGQL
                     AAPLLAPEPGDRVLDACCAPGGKTGHLLETALGLELLALDIDATRLVRVRENLDRLGY
                     DAELLAADAAAPADWWDGRPFERILLDAPCSATGVIRRHPDIKALRQPADIDALAARQ
                     RALLDALWPLLAPGGILLYATCSLLRAENETVIAAFLAAHPDAAELVIDADWGLALRH
                     GRQILPGSAGLDGFYYARLLRTP"
BASE COUNT          488 a         1112 c         1212 g          422 t
ORIGIN      
        1 cctgggcgcg gtacaggtcc gccacctcgg tgaccagctg gttgaggctg aacggcgcga
       61 cgctcatggt cggcgcgcgc gcgtactcgc tgaaggcgtt gaccatggcc ttcatcgcct
      121 cgacctgctg cacgatggtg tgggtcgcgc ggtccatgac ctttgcgtcc tcctcgctca
      181 tgttgccgag gaagcgccgg cgcatccgct cggcggacag ctggatcggc gtcagcgggt
      241 tcttgatctc gtgcgcgagg cggcgcgcga cctcggtcca ggcggcgtcg cgctcggcct
      301 gcaccagcgt cgtgatgtcg tcgaacacca gcacgtgccc gcccttgagg ccgacggcat
      361 ccggcagccg cgagccgcgg cacatcagca cgcgccgccc gccgggggcg aacagcgtga
      421 cctcctgccg ccattcggcg ctctcgccgg cgagatgtcc ggcgacggcg ccgagcaggt
      481 gcgcgagcgg cgattcgccc tcggccgaca gcgggtggcc ggcatcgagg ccgaggatgt
      541 gcagcgtcgc ggcgttgaag gtgcgcgggt ggccgtcgtg atcgagcgtc agcacgcccg
      601 aggacaggcg cgcgagcacg gtttcgaggt aggcgcgctg accttcgagc atgtgccggc
      661 tctgcagcgc cgagtcgcgc gcgctggcga ggttgcgcgt catctcgttg aaggactgca
      721 cgaggaagcc gagctcgtcg ttggccgccg gtgcgagctg cttgtcgtac tggccggcgg
      781 cgactgcgcg cgtgccgtcg gcgaggtcgc gcaccggctg cacgaacttg cgcgcgaagt
      841 acacggccgc cgacagcgca ctcagcacgc cgaacagcag caccagcgac agcgtcatga
      901 tgaaggtcgt gcgcagcggc tcgcgcagga agaccagttc gcggtaggca tcgaaggccg
      961 cctgcaccga atgcccgagc gagttgaagc ggtcggtgac cgggtacagc gcctgcagca
     1021 gcaggtcatc gccgatggtc gcgtgcgccg gcaccggcac gaccgcgcgc agctggaagc
     1081 cgagttcggc ggtcgggtcg agcgcgacgt agttgccgcc ctggcgcagc tgcgcgagca
     1141 cgacatccgg cgggcgctcc ggcaccaggc tcgcggcctc gctggcggcg accaccagcg
     1201 gcgtgccgtc ggccttcagc acggccagct cctgcgcatc gctctcctcg cgcagctccg
     1261 cgagcagcag cgtcgacggc gggcccgggg tctcgccgag ctggccggcg aggcgcacgg
     1321 tctgcttgag cacctcgcgc atgcgcaggt cgagcgcggt gcgcgacagt tcgagggcat
     1381 cctccagcgc cttctcgacg cgcacgtcga accacgaatc gatcgagcgc gcgatgaact
     1441 gcaccgagaa gtaatacacg accgacaccg gcgcgaccga gatcagcgag aacagcacga
     1501 acagccgcag cgtcagccgc gagccgggtt cgtcgttgcg cacctgccgg tacagatccc
     1561 agaggttgcg cccgatcagg aacgcgagca gcacgaagcc gagcgcgctg ccgaccagca
     1621 gccacgagta cagctcgccg aagcgcgcgg agttgccggt cgcctcgccc atcagcgcca
     1681 gcgccaccag cagcaccgcc agcagcgccg cgttgaccag caggccgcgc actgcaatcc
     1741 gccgcatgct catgacgacc tcatagcgac cacgcgtacc aggtcgagcc gagccgccag
     1801 tccgtcgaca ggtaggcgac cgggcgcagc ggtggcggca gcgcctcgat gtcgaggctc
     1861 gcgcgcagcc gcccgtaata gttgccgggg ggcttgagca gcgcccggtc gatcagcggg
     1921 aagtcgtgca gccggccgat ggcatcgagc gcgccggcaa gcgtcgggaa gctgcgcagg
     1981 tcattggtgt tgaggttcac ggccacgtac tggcgcgcga gcgcgtggta ttcgagccgg
     2041 aagcgctggc tgaggctggc gacggtctcg tcccacagca gatcgcggtc gcggacgatc
     2101 tcgacatcga tctcgatggt gagcggcacg ccgttgcgca gcgcatcgag cggggcctcg
     2161 gtgaactgca ggtcgatcag cgcgtcgagg cgcagcaggc cgcgctcgct ggtcgaactg
     2221 gcgcgcagca cgcggaagcc gtcggcggcg gccggcaccg gcgcgagcca cagacacagc
     2281 agcccgagca gcagcgcgag gcacggggtc cggtggcggt cggtgaaagc ggtcacgggg
     2341 tcctgagcag gcgtgcgtag tagaagccgt cgaggccggc gctgcccggc aggatctggc
     2401 ggccgtggcg cagcgcgagc ccccagtccg cgtcgatgac gagttccgcg gcatcggggt
     2461 gggcggcgag gaaagccgcg atcacggtct cgttttcggc gcgcagcagc gagcaggtgg
     2521 cgtacagcag gataccgccc ggggccagca gcggccacag cgcatcgagc agcgcgcgct
     2581 ggcgggcggc gagcgcgtcg atgtcggccg gctggcgcag cgccttgatg tcgggatggc
     2641 ggcggatcac gccggtggcc gagcacggcg catcgagcag gatgcgctcg aagggccggc
     2701 cgtcccacca gtctgccggt gccgcggcat cggcggcgag cagttcggcg tcgtagccga
     2761 gccggtcgag gttctcgcgc acgcgcacga ggcgcgtggc atcgatgtcg agcgccagca
     2821 gttcgaggcc gagtgcggtt tcgagcaggt ggccggtctt gccgccgggc gcgcagcagg
     2881 catcgagcac gcgatcgccc ggctcgggcg cgagcagcgg cgcggcgagc tggccggcgc
     2941 cgtcctgcac cgacagccgg ccatcggcga agccgggcag ggcgctgacc tcggcggggt
     3001 gctccagcac gacggcactc gccacctgcg cgtgtgccgc ggcggcgatg ccggccgtcg
     3061 cgagccgcgc cagtgcctcg tcgcggcttt cacgcgccaa gttcacgcgc agcatcatcg
     3121 gggcctgctg gttgctggcc tcgacgatcg ccggccagtc cgcgggccag tcggcctgca
     3181 gccgctccag cagccaggcc ggatgcgcgt gcacggccac cggctgggcg gcca
//
LOCUS       DAOQJH010000302         3231 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_19525_length_3231_cov_3.823048, whole genome shotgun sequence.
ACCESSION   DAOQJH010000302 DAOQJH010000000
VERSION     DAOQJH010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3231
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19525_length_3231_cov_3.823048"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..241)
                     /locus_tag="PLQ18_07740"
     CDS             complement(<1..241)
                     /locus_tag="PLQ18_07740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE01582.1"
                     /translation="MSGVVMPDRSRRQPFWFSGGIGRASIRKSIRPPTLADVGGLMER
                     RGGADRAPEEAAQPLRHPQAGGSEHEPVGVAGAAAG"
     gene            260..1138
                     /locus_tag="PLQ18_07745"
     CDS             260..1138
                     /locus_tag="PLQ18_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011517883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HNE01583.1"
                     /translation="MRYGDDDRESGNIEDRRGESFGGGGGFGGGGLRIGGGIGTVVLV
                     LVALYFGVDPSVILGGGSPAPVQPPPGPAQTRAHDDEARFVGRVLADTESVWNAQLPA
                     QTGQAYVEPKLVLFSGATQTACGIGQAAMGPFYCPPDRKVYIDLSFYDDLRQRFRAPG
                     DFAQAYVVAHEIGHHIQNLLGISGRVHAARERMSEADGNRLSVRLELQADCLAGVWAR
                     HAEQTKHRLEPGDVEEALTAAAAIGDDRLQRQARGTVVPDSFTHGSSQQRVRWFRAGL
                     DSGRLDACDTFGAARL"
     gene            1241..>3231
                     /locus_tag="PLQ18_07750"
     CDS             1241..>3231
                     /locus_tag="PLQ18_07750"
                     /inference="COORDINATES: protein motif:HMM:NF025181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tubulin-like doman-containing protein"
                     /protein_id="HNE01584.1"
                     /translation="MNHVIVGLGGTGGKVIRALRKLIFQEFRSEEPEGVPLGWLYVDS
                     SDELMAPDDASWKTLGVSVQLPAASQLLITDANLAARLDDLDSYPGIKPWIGAREQWR
                     DILNSIVGVTLGGQKRRLGRFLFACKADKYREQLQTQVRRVQAGGSSETTFHVLCGLA
                     GGTGSGSLIDALVQLRELFPDAQRYRVLVYALLPDPYPPPNWDTGNYHANGYAALLEL
                     NALSAGAWQPHDVLGRKGRLALVDPFNGCYVFGNENEHGVTVDVQQELPGIVAEFLYQ
                     KIIRARHGGWPTLARMENAENGDGSPETAPASRRGERARRFLTFGIKRVAIPEEEIAE
                     FLGYGFARQALLALAHEHWQEALGYVEEPRPFDAGAFVRQKDVQQRWALDDDHLLMNV
                     PILAGDGQAPKWQTLTLEWETAIPHFKSLVRELDRTIWLDELAKYCQKRWDEDFRDLG
                     VTGFYETKLKARKDIAREIANRIERELANDWRIGARGLDACTRLVAAVIELLDERHAQ
                     LGERIARARAGEDEAQTRLNQNARDWAGMGVFAKLTGTPASLLDAHAVYLQELYVNRT
                     RGVALRAQQQLLPELIAALAELKTAIERAAGSLRTALERFGQRLEQRLADGATATGSD
                     LRRHLIRYYDPARVREVAHRLGLDAEVQRGQAAGARAAL"
BASE COUNT          472 a         1169 c         1156 g          434 t
ORIGIN      
        1 cgccggcggc agcgccggcg acacccaccg gttcgtgttc tgatccgccc gcctgcgggt
       61 gcctgagcgg ctgcgccgct tcctccggcg cccggtccgc cccgccccgc cgctccatca
      121 ggccgccgac atccgcgagg gtcggcggcc tgatgctttt ccggatcgat gcccggccga
      181 tgccgccact gaaccagaac ggttggcggc ggctccgatc cggcatcacg acaccggaca
      241 tccgggggag gcgaacgtca tgcgttacgg cgacgacgac cgcgagagcg gcaacatcga
      301 ggaccgccgc ggtgaatcct tcggcggggg cggcggcttc ggcggcggcg ggctcaggat
      361 cggcggcggc atcggcaccg tcgtgctggt gctggtggcg ctgtacttcg gggtcgaccc
      421 gtcggtgatc ctcggcggcg gttcgccggc gccggtgcag ccgccgccgg ggccggcgca
      481 gacgcgcgcg cacgacgacg aggcgcgctt cgtcggccgt gtgctcgccg acaccgagag
      541 cgtctggaac gcgcagctgc cggcgcagac cgggcaggcc tacgtcgagc cgaagctcgt
      601 gctgttctcc ggtgccacgc agaccgcctg cggcatcggc caggcggcga tggggccgtt
      661 ctactgtccg ccggatcgca aggtctacat cgacctgtcc ttctatgacg acctgcgcca
      721 gcgcttccgc gcgcccggcg atttcgcgca ggcttacgtc gtcgcgcacg agatcggcca
      781 ccacatccag aacctgctcg gcatctccgg gcgcgtgcac gcggcgcgcg agcgcatgag
      841 cgaggccgac ggcaaccggc tgtcggtgcg gctcgaactg caggccgact gtctggccgg
      901 cgtctgggcg cggcacgccg agcagacgaa gcatcgcctc gaacccggcg atgtcgagga
      961 agcgctgacc gccgctgccg cgatcggcga tgaccgcctg cagcgtcagg cccgcggcac
     1021 ggtcgtgccc gacagcttca cgcacggcag ctcgcagcag cgcgtgcgct ggttccgggc
     1081 cgggctggat tcggggcgcc tcgacgcctg cgataccttc ggcgccgcac ggctgtagcc
     1141 cgcgcgcccg gcgccgcgcc ggcgtcgcgc atccgccgcg cttgccctgt cccgccccgc
     1201 cgggcatggt tcgcccgaca gcggcggccg ggaggcaggc atgaatcatg tgatcgtcgg
     1261 gctcgggggc acgggcggca aggtcatccg cgcgctgcgc aagctgatct tccaggagtt
     1321 ccgcagcgaa gagcccgaag gcgtgccgct cggctggctg tacgtcgatt ccagcgacga
     1381 gctgatggcg ccggacgatg cctcgtggaa gacgctcggc gtctcggtgc agctgccggc
     1441 ggcgagccag ctgctgatca ccgacgccaa cctggcggcc cggctcgatg acctcgacag
     1501 ctaccccggc atcaagccgt ggatcggcgc gcgcgagcag tggcgcgaca tcctcaacag
     1561 catcgtcggc gtcacgctcg gcggccagaa gcgccggctc gggcgcttcc tgttcgcgtg
     1621 caaggccgac aaataccgcg aacagctgca gacgcaggtg cgccgcgtgc aggccggcgg
     1681 cagcagcgag acgaccttcc acgtgctgtg cgggctcgcc ggcggcaccg gcagcggcag
     1741 cctgatcgat gcgctcgtgc agctgcgcga actgttcccg gatgcgcagc gctaccgcgt
     1801 gctggtctac gcgctgctgc ccgatcccta cccgccgccg aactgggata ccggcaacta
     1861 ccacgccaac ggctacgcgg cgctgctgga gctgaacgcg ctgtcggccg gggcctggca
     1921 gccgcacgac gtgctcgggc gcaaggggcg gctcgcgctc gtcgatccgt tcaacggctg
     1981 ctacgtgttc ggcaacgaga acgagcacgg cgtcaccgtc gacgtgcagc aggagctgcc
     2041 gggcatcgtc gccgagttcc tgtaccagaa gatcatccgc gcccggcacg gcggctggcc
     2101 gacgctcgcg cgcatggaga acgccgagaa cggcgacggc tcgccggaaa ccgcaccggc
     2161 gagccgtcgc ggcgagcgcg cccggcgctt cctgaccttc ggcatcaagc gtgtcgcgat
     2221 ccccgaggag gagatcgccg agttcctcgg ctacggcttc gcgcgtcagg cgctgctcgc
     2281 gctcgcgcac gagcactggc aggaggcgct cggttacgtc gaggagccgc ggcccttcga
     2341 tgccggcgcc ttcgtgcgcc agaaggacgt gcagcagcgc tgggcgctcg atgacgatca
     2401 cctgctgatg aacgtgccga tcctcgccgg cgacgggcag gcgccgaaat ggcagacgct
     2461 gacgctggag tgggagacgg cgatcccgca cttcaagtcg ctggtgcgtg agctcgatcg
     2521 cacgatctgg ctcgacgagc tggcgaagta ctgccagaag cgctgggacg aggacttccg
     2581 cgacctcggc gtcaccggct tctacgagac caagctcaag gcgcgcaagg acatcgcccg
     2641 cgagatcgca aaccgcatcg agcgcgaact cgccaacgac tggcgcatcg gcgcacgcgg
     2701 cctcgatgcc tgcacgcgcc tcgtcgccgc cgtcatcgag ctgctcgacg agcgccacgc
     2761 gcaactcggc gagcgcatcg ccagggcgcg cgccggcgag gacgaggcgc agacccggct
     2821 caaccagaac gcccgcgact gggccggcat gggcgtgttc gcgaagctga ccggcacgcc
     2881 ggcgagcctg ctcgacgcgc acgccgtgta cctgcaggag ctgtacgtca accgcacgcg
     2941 cggcgtggcg ctgcgtgcgc agcagcaact gctgccggag ctgatcgcgg cgctggcgga
     3001 actcaagacc gcgatcgagc gcgccgccgg cagcctgcgc acggcgctgg agcgcttcgg
     3061 ccagcgcctc gaacagcgcc tcgccgacgg cgcgaccgcc accggctccg acctgcgccg
     3121 gcacctgatc cgttactacg atccggcgcg cgtgcgcgag gtcgcgcacc gcctcggcct
     3181 ggatgccgag gtgcagcgtg gtcaggcggc cggcgcgcgc gcggcgctgt t
//
LOCUS       DAOQJH010000303         3225 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_19578_length_3225_cov_2.814196, whole genome shotgun sequence.
ACCESSION   DAOQJH010000303 DAOQJH010000000
VERSION     DAOQJH010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3225)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3225)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3225
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19578_length_3225_cov_2.814196"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1848)
                     /locus_tag="PLQ18_07755"
     CDS             complement(<1..1848)
                     /locus_tag="PLQ18_07755"
                     /inference="COORDINATES: protein motif:HMM:NF040143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5682 family protein"
                     /protein_id="HNE01585.1"
                     /translation="MPAEVHLLGIRHHGPGCARAVLAALDALSPTLLLVEGPPEADGL
                     IGRIGDPALEAPVALLAHVADDPARAVFYPFAQFSPEWQALRWALEHGVPARFCDLAL
                     AVSMAEPKGAAGEADAEGETAAPADDAPPACEAAPACARWRDPFDSLAELAGFDDGEA
                     WWEYHVERSGAGTELALFSAVAEAVTALRAHADPHADDPWQAPREAAMREAIRAAQRE
                     GHERIAVVCGAWHVPALAAPLPPAKADKALLAGRAKVKIASTWVPWTHERLGLASGYG
                     AGIRVPGWYEHLWQHGEDAPLRWLARCAQLLRAHDLAAPTAQLIDALRLAEALAALRG
                     RARPDLPDLEQAAHATLCAGESLALTLIRRELEVGDRLGRVPDDVPAPPLARDLASEQ
                     KRLRLAPSAAHRDLDLDLRQPLDRERSCLLHRLRLLDLPWGEPRAGAPRGRGTFHEHW
                     QLIWRPEFAVNLIEAGRWGQTVAAAAAACALDAITRASGLAGLVDWLDRVLLAELPEA
                     AGRLLARVRDAAAQSGDVDALLDALLPLARLARYGDARERDAQQFLDVLHALLARIAI
                     NLPPAATGLDDEAATTLFGRINAVEAALATLADARAEADWRAALQALADH"
     gene            complement(1869..2972)
                     /locus_tag="PLQ18_07760"
     CDS             complement(1869..2972)
                     /locus_tag="PLQ18_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004984297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNE01586.1"
                     /translation="MSTTPAPVLRAHAEQLYAEELAALARLDERPRPPNWRLSPQAVV
                     TYLLGGRLADGSEVVPKYVGERRLIEVAVATLATDRALLLYGVPGTAKSWVSEHLAAA
                     ISGDSTCLIQGTAGTPEEAIRYGWNYARLLADGPSPAALVESPLLRAMRSGTLCRIEE
                     LTRIPSDVQDSLITVLSEKTLPVPELDSEIQAVRGFNVIASANNRDKGVNELSSALKR
                     RFNTVVLPLPDSLDTEVAIVAQRAASLGRALELPAEPPAIEEIRRIVTIFRELRAGQT
                     LDGRTRLKAPTGTLSTAEAISVVGHGLALAAHFGDGRLRAADWAASIEGAVIKDPVQD
                     TLVWREYIETVARERDGWKDVYRACRDNGGWPA"
     gene            complement(2969..>3225)
                     /locus_tag="PLQ18_07765"
     CDS             complement(2969..>3225)
                     /locus_tag="PLQ18_07765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE01587.1"
                     /translation="SQPELFVPLRAFLASRLPQTVHDHYAWSQLRAWLRLVPVAPALA
                     ALRDWRLHAAPELYPRYDDLLALLERRVHLPVAFLPAEPT"
BASE COUNT          419 a         1159 c         1233 g          414 t
ORIGIN      
        1 gtgatcggcg agtgcctgca gcgcggcgcg ccagtcggcc tcggcgcgcg catcggcgag
       61 cgtcgccagg gccgcctcga cggcgttgat gcgcccgaac agcgtcgtcg cggcttcgtc
      121 atcgaggccg gtcgcggccg gcggcaggtt gatcgcgatg cgcgcgagca gcgcgtgcag
      181 cacgtcgagg aactgctgcg cgtcgcgctc gcgggcatcg ccgtagcggg cgaggcgcgc
      241 gagcggcagc agcgcatcga gcagcgcatc gacatcaccg ctctgcgccg cggcatcgcg
      301 cacgcgcgcg agcagccgcc cggcggcctc tggcagctcg gcgagcagca cgcgatcgag
      361 ccagtcgacc agcccggcga ggccgctggc gcgcgtgatc gcatcgagcg cgcacgccgc
      421 ggcggccgca gcgaccgtct ggccccagcg cccggcctcg atcaggttca ccgcgaactc
      481 cggccgccag atcagctgcc agtgctcgtg gaaggtgccg cgcccgcgcg gcgcgccggc
      541 gcgcggctcg ccccagggca ggtcgagcag gcgcagccgg tgcagcaggc aggagcgttc
      601 gcgatcgagc ggctggcgca ggtcgaggtc gagatcgcgg tgcgcggcac tcggcgcgag
      661 ccgcaggcgc ttctgttcgc tggcgagatc gcgcgcgagc ggcggggccg gcacgtcgtc
      721 gggcacacgc ccgagccggt cgccgacttc gagttcgcgg cggatcagcg tgagcgcgag
      781 cgactcgccg gcgcacagcg tcgcgtgcgc cgcctgttcg agatcgggca gatccggccg
      841 ggcgcgtccg cgcagcgccg ccagcgcctc ggcgaggcgc agcgcgtcga tcagctgcgc
      901 ggtcggtgcc gccagatcgt gggcgcgcag caactgcgcg cagcgcgcca gccagcgcag
      961 cggcgcatcc tcgccgtgct gccacaggtg ttcgtaccag ccgggcacac ggatgccggc
     1021 gccgtagccg ctcgcgagtc cgagccgctc gtgcgtccac ggcacccagg tgctcgcgat
     1081 cttcaccttc gcccggccgg cgagcagcgc cttgtcggcc ttcgccggcg gcagcggtgc
     1141 ggcgagggcc ggcacgtgcc aggcgccgca gacgacggcg atgcgttcgt gcccttcgcg
     1201 ctgcgcggcg cggatcgcct cgcgcatcgc cgcctcgcgc ggggcctgcc acgggtcatc
     1261 ggcatgcgga tcggcgtgcg cgcgcagcgc cgtcaccgcc tcggcgaccg cggagaacag
     1321 cgccagctcc gtcccggccc cgctgcgctc gacgtggtac tcccaccacg cctcgccgtc
     1381 gtcgaagccg gcgagttcgg cgagggagtc gaaggggtcg cgccagcgcg cgcatgccgg
     1441 cgcggcttcg cacgcaggcg gtgcgtcgtc agccggtgct gcggtctcgc cttcagcgtc
     1501 ggcctcgccg gctgcgccct tcggctccgc catcgacacg gcgagcgcca gatcgcagaa
     1561 acgcgccggc acgccgtgtt cgagcgccca gcgcagcgcc tgccattccg gcgagaactg
     1621 cgcgaacggg tagaacacgg cacgcgccgg atcgtcggcg acgtgcgcga gcagcgcgac
     1681 cggcgcttcg agcgccggat cgccgatgcg cccgatcagg ccgtcggcct cgggcggccc
     1741 ttcgaccagc agcagcgtcg gcgacagcgc atcgagcgcc gcgagcaccg cgcgcgcgca
     1801 gccggggccg tggtggcgga tgccgagcag gtggacttcg gccggcatgg tcggtcagcc
     1861 ccgccggttc aggccggcca gccgccgttg tcgcggcagg cccggtagac atccttccag
     1921 ccgtcgcgct cgcgggcgac ggtctcgatg tattcgcgcc agaccagcgt gtcctgcacc
     1981 gggtccttga tcaccgcgcc ctcgatgctc gccgcccagt cggcggcgcg caggcggccg
     2041 tcgccgaagt gcgcggcgag cgcgaggccg tggccgacga ccgagatcgc ctcggcggtc
     2101 gacagcgtgc cggtcggggc cttgagccgg gtgcggccgt cgagcgtctg gccggcgcgc
     2161 agttcgcgga agatcgtgac gatgcggcgg atttcctcga tcgccggcgg ctcggccggc
     2221 agttcgagcg cgcgcccgag gctggcggcg cgctgcgcga cgatcgcgac ctcggtgtcg
     2281 agcgaatccg gcagcggcag cacgacggtg ttgaagcgcc gcttcagcgc gctcgacagc
     2341 tcgttgacac ccttgtcgcg gttgttggcg ctggcgatga cgttgaagcc gcgcacggcc
     2401 tggatctcgg aatcgagttc cggcaccggc agggtctttt ccgacagcac ggtgatcagg
     2461 ctgtcctgca cgtcggacgg gatgcgggtg agttcctcga tgcggcacag cgtgccgctg
     2521 cgcatcgcgc gcagcagcgg gctttcgacc agtgctgccg gcgacgggcc atcggcgagc
     2581 aggcgcgcgt agttccagcc gtagcggatg gcttcctcgg gcgtaccggc cgtgccctgg
     2641 atcaggcagg tcgaatcgcc gctgatcgcc gcggccagat gctccgagac ccagctcttg
     2701 gcggtgccgg gcacgccgta gagcagcagc gcacggtcag tcgcgagcgt tgcgaccgca
     2761 acctcgatca gccgccgctc gccgacgtac ttcggcacga cttcgctgcc gtcggcgagc
     2821 ctgccaccga gcaggtaggt gacgacggcc tgcggcgaca gccgccagtt cggcgggcgc
     2881 ggacgctcgt cgaggcgcgc gagcgcggcg agttcttcgg cgtacagctg ttcggcgtgc
     2941 gcgcgcagca ccggcgcagg ggtcgtgctc atgtcggctc cgcaggcagg aaggcaacgg
     3001 gcaggtgcac gcgccgttcg agcagcgcga gcaggtcgtc gtaacgcgga tacagctcgg
     3061 gcgcggcgtg caggcgccag tcgcgcagcg ccgcgagtgc cggcgcgacc ggcacgaggc
     3121 gcagccacgc gcgcagctgc gaccaggcgt aatggtcgtg aacggtctgc ggcaggcgcg
     3181 aggcgaggaa ggcgcgcagc ggtacgaaga gttccggctg cgagg
//
LOCUS       DAOQJH010000304         3222 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_19609_length_3222_cov_4.566151, whole genome shotgun sequence.
ACCESSION   DAOQJH010000304 DAOQJH010000000
VERSION     DAOQJH010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3222
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19609_length_3222_cov_4.566151"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(135..1118)
                     /locus_tag="PLQ18_07770"
     CDS             complement(135..1118)
                     /locus_tag="PLQ18_07770"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HNE01588.1"
                     /translation="MSISPDDPEPMYRIGTVSRLTGIATVTLRMWERRYNVVDPGRSS
                     GRNRLYSRDDIARLALIKRLVDAGHSISTVAGLTLVQLHERLEVQEKPRQLAALPVRV
                     LTPVRVAVLGDALPARIARAGSELAGLAIVASQRERARFLADAPAQEPQVLVIEVPAL
                     GPETAAGVEELRQACGARHVVIVYGFGRRATVAKLDTAYSTPLRAPVGMAELRIACRA
                     GLGVAALPLPERQPVDLTPSPDGIPQRRYDTDTLERIAASTPTLACECPHHLVDLVAG
                     LSAFEDYSAQCENRSPEDAALHHWLRATTAQARALMEEALTRVAEAEGLLR"
     gene            complement(1159..2256)
                     /locus_tag="PLQ18_07775"
     CDS             complement(1159..2256)
                     /locus_tag="PLQ18_07775"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004634965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HNE01589.1"
                     /translation="MKAVLALDPLLPLRSAAAGVGQRFDALAIELAERGWLPDAVLRA
                     GIRRLLAARLDSEARRVAQRTAWIESLHAGPIALATDAANRQHYEVPAAFFEHVMGRH
                     LKYSCGFWPEGVHTLDEAEAAMLALTCERAALADGQDILELGCGWGSLTLWMAERYPH
                     ARITAVSNSRTQRRYIQSRLRARGLRNVQLITADMNDFASGPSLNGAFDRVVSVEMFE
                     HMHNWPALLACVKGWLRAEGRLFLHVFCHRRYSYPFVDEGSDDWMARHFFTGGLMPGA
                     DLPMRFQDDLQLARHWWIDGRHYQATCEAWLAGQDRRRDEILRLFAADGDPAEAPRQF
                     RRWRLFFLACSELFGYREGSEWGVGHYLYTR"
     gene            complement(2410..3159)
                     /locus_tag="PLQ18_07780"
     CDS             complement(2410..3159)
                     /locus_tag="PLQ18_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010485798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HNE01590.1"
                     /translation="MTLAWAHQQRTHDATAVDVCWAAGMGGLAIAAALLAGGDPARRT
                     LVALLGGLWSARLAGFLYRNRVRGHAGEDARYRRLRAAFGRHAGAGFLAFYQGQALAT
                     ALFALPFLACAQAQAPLSGPAAIAALLVWALAVGGESLADRQLARWRAEPAHRGRTCR
                     TGLWAWSRHPNYFFEWLHWWTYPLLALGSEAFWPTLAGPLLMYATLHYATGIPHTERQ
                     ALAHRGADYAAYQREVPEFFPRPPRPPERGA"
BASE COUNT          474 a         1137 c         1201 g          410 t
ORIGIN      
        1 gcgcgcggag agggtcttcg cgcgctcgga cgtcacggcg agcagcgcat cgagccgttc
       61 gcagtccgga ccggcggctt gcgccagcgc ggcatccgat gcccatgtgg ccgcggcggc
      121 ccggtcggcg gcgctcagcg cagcagccct tcggcctcgg cgacgcgggt cagggcctct
      181 tccatcagcg cccgcgcctg cgcggtggtc gcgcgcagcc agtgatgcag cgccgcgtcc
      241 tcggggctgc ggttctcgca ctgcgcgctg tagtcctcga aggccgacag cccggccacg
      301 agatcgacga ggtgatgcgg gcattcgcag gcgagcgtcg gcgtcgaggc ggcgatgcgc
      361 tccagtgtgt cggtgtcgta gcgccgctgc gggatgccgt ccggcgacgg cgtcaggtcg
      421 accggctggc gctcgggcag cggcagcgcc gcgacgccga gcccggcgcg gcaggcgatg
      481 cgcagctccg ccatgccgac cggcgcgcgc agcggcgtgc tgtaggcggt gtcgagcttc
      541 gcgaccgtcg cgcgccggcc gaagccgtag acgatgacca cgtgccgcgc gccgcaggcc
      601 tggcgcagtt cctcgacccc ggcagccgtc tccggcccga gcgccggcac ctcgatgacc
      661 agcacctgcg gctcctgcgc cggggcatcg gcgaggaagc gcgcgcgctc gcgctggctg
      721 gcgacgatgg caaggcccgc cagttcgctg ccggcgcggg cgatgcgcgc cggcagcgca
      781 tcgccgagca cggccacgcg caccggcgtc agcacccgca ccggcagtgc cgcgagctgc
      841 cggggcttct cctggacttc gaggcgctcg tgcagctgca cgagcgtcag gccggcgacg
      901 gtgctgatcg agtgtccggc atcgaccaga cgcttgatca gcgccagccg ggcgatgtcg
      961 tcgcgcgagt acagccggtt gcgaccgctg gagcgaccgg gatcgacaac gttgtagcgc
     1021 cgctcccaca tgcgcagcgt gaccgtggcg atgccggtca ggcgggagac ggtgccaatg
     1081 cggtacatcg gttcgggatc gtcgggtgaa atgctcatga cggcttgtct cggatgccgg
     1141 acatccatgg ccgcaggctc agcgggtgta caggtagtga ccgacacccc attcactgcc
     1201 ctcccggtaa ccgaacagtt cgctgcacgc cagaaagaac aggcgccagc gccggaactg
     1261 ccggggggct tcggccggat caccgtcggc ggcaaacagc cgcaggattt cgtcgcgccg
     1321 gcggtcctgg ccggcgagcc aggcctcgca ggtcgcctgg tagtgacgcc cgtcgatcca
     1381 ccagtgacgg gccagttgca ggtcgtcctg gaaacgcatc ggcagatccg cgcccggcat
     1441 caggccgccg gtgaagaaat gtcgtgccat ccagtcgtca ctgccttcgt ccacgaacgg
     1501 ataactgtag cgtcgatggc aaaagacgtg caggaacaat cgcccttcgg cgcgcagcca
     1561 gcccttcacg cacgcgagca gcgccggcca gttgtgcatg tgctcgaaca tctcgaccga
     1621 cacgacgcga tcgaacgccc cgttcaggga gggcccgctg gcgaagtcgt tcatgtcggc
     1681 cgtgatgagc tgcacgttgc gcaggcctct tgcacgcagg cgcgactgga tgtagcgccg
     1741 ctgcgtgcgc gagttcgaca ccgccgtgat gcgtgcgtgc gggtagcgct cggccatcca
     1801 cagcgtcagc gagccccagc cgcagccgag ttcgaggatg tcctgaccat cggcgagcgc
     1861 ggcgcgctcg caggtcaggg ccagcatcgc cgcctcggcc tcgtcgagcg tgtgcacgcc
     1921 ctcgggccag aagccgcagg agtacttcag gtgccggccc atcacgtgtt cgaagaaggc
     1981 cgccggcacc tcgtagtgct ggcggttggc ggcgtcggtt gcgagcgcga tcgggccggc
     2041 gtgcagggat tcgatccagg ccgtgcgctg cgcgacgcgg cgcgcctcgc tgtcgagccg
     2101 cgcggcgagc agccggcgga tgccggcgcg cagcacggca tcgggcagcc agccgcgctc
     2161 ggccagttcg atcgcgaggg catcgaaacg ctggccgaca ccggccgcgg cactgcgcag
     2221 cggcagcagg ggatcgaggg cgaggacggc tttcatcgga acatctcctg cgggcacgga
     2281 cggggacggg gacggggcga cggtcgccgg gccgggcacg cagcagcgcc tcgctttcca
     2341 gccgccgcgc cccccatgga agaggcgccg tggagcggcg gaacccgggg catctgcgcg
     2401 tgaccggtct caggcacctc tttcgggagg gcgcggcgga cgggggaaga attccggcac
     2461 ctcgcgctgg taggcggcgt aatcggcgcc gcggtgcgcg agcgcctggc gttcggtgtg
     2521 cgggatgccg gtggcgtagt gcagcgtcgc gtacatcagc agcggtccgg ccagcgtcgg
     2581 ccagaaggcc tcgctgccga gcgcgagcag cggataggtc caccagtgca gccattcgaa
     2641 gaagtagttc gggtgtcgcg accaggccca cagcccggtg cggcaggtcc ggccccggtg
     2701 cgccggctcg gcccgccagc gcgcgagctg ccggtcggcc agggattcgc cgccgaccgc
     2761 gagcgcccag accagcagcg cggcgatggc ggccggcccg gacagcggcg cctgcgcctg
     2821 cgcgcaggcg aggaagggca gcgcgaacag cgccgtggcc agcgcctgcc cctgatagaa
     2881 ggccaggaag ccggcgccgg cgtgcctgcc gaaggccgcg cgcaaccgcc ggtagcgggc
     2941 atcctcgccg gcgtggccgc gcacgcggtt ccggtacagg aaaccggcaa gacgtgccga
     3001 ccacaggccg ccgagcagcg cgaccagcgt ccggcgcgcc ggatcaccgc cggcgagcag
     3061 cgccgccgcg atcgcgaggc cgcccatgcc ggcggcccag cagacatcga cggccgtggc
     3121 atcgtgcgtg cgctgctggt gcgcccacgc gagcgtcatc accagcgccg cggccgccca
     3181 gccgccgagg aacagggccg tgacgctcac cggccatgcc tc
//
LOCUS       DAOQJH010000305         3217 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_19661_length_3217_cov_4.041429, whole genome shotgun sequence.
ACCESSION   DAOQJH010000305 DAOQJH010000000
VERSION     DAOQJH010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3217
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19661_length_3217_cov_4.041429"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..2466)
                     /gene="glnE"
                     /locus_tag="PLQ18_07785"
                     /pseudo
     CDS             complement(1..2466)
                     /gene="glnE"
                     /locus_tag="PLQ18_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637050.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
     gene            complement(2686..>3217)
                     /locus_tag="PLQ18_07790"
     CDS             complement(2686..>3217)
                     /locus_tag="PLQ18_07790"
                     /inference="COORDINATES: protein motif:HMM:NF017870.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF945 family protein"
                     /protein_id="HNE01591.1"
                     /translation="MEFLPHHPVLAFENVHIEKTGEPLPLDARLALTLQPPTDPSAPD
                     AALQYLQAAQGQVAVPESFVLALVTEQVHTAMANEAEMAEMERRMQQEEAGAQADPDR
                     PEAGTEPDTPLPEPDPQQVAERARQVYDENLAQLLAQGWLKRADGRLSTVIDYRERKL
                     TLNGVELPVAQMLGGE"
BASE COUNT          438 a         1157 c         1185 g          437 t
ORIGIN      
        1 gtgctcgcgc atcttctcgc gcatctcgcg cacctcgcgg cgcagcacgg ccggttcgcg
       61 cacgcgggcg aggacttcct gacgcagcgc gcagaagcgc tcggcgatcg ccgccgagcc
      121 ggtcacggga cgcgcgcgca ccagcgcctg atgctcccag gtccaggccc cggcgcgctg
      181 gtaggcggcg tagccgtcga tgcttgcgac catcatgccg gcgcgaccgc tcgggcgcag
      241 gcgggtgtcg atctcgtaga gcacgccggc cccggtcggc gcggtcagca cgtggatcag
      301 gcgctgcgcg agccgcgcga agaacgtggc gttgtcgagc ggacgctcgc cgtcggtttc
      361 ctgctcctcg ccggcggaat cgtgcaggaa caccagatcg aggtccgagc cgtagccgag
      421 ttcgaggccg ccgagcttgc cgtaggcgat gatcgcgaag ccggcggtgc gcgcaacgcc
      481 gtcgacgacg cagcgcggca ggccgtggcg gacggtgacg tcggcccagg cctgcaccag
      541 cgcctcgtgc agcagggtct cggcgatcgc ggtcaggtga tcgctgacca ccatcagcgg
      601 cagtgccccg ctcacgtcgg cggcggcgac gcgcagcacg ttgatctgct tgaactggcg
      661 cagcaccagc aggtgcgcct cgtgatcgcc ttcgggcacg cgcgacagca tctgccggac
      721 ctcggcggcg agcgcctcgg ggccgggcgg cgcatagagc acggccgggt tcagcagctc
      781 atcgagcagc accgggaagc gcgtcagctg ctcggcgatc cacgggctgg ccccggcgag
      841 cgcgaccagt tggcccagcg ccacgggctt ctcgtgcagc agcgccagat acaccgagcg
      901 gcgcgcgacg gcttcgagca gcggtgccag cctcgccagc gtcaccgccg gctgcgggcc
      961 gttgccggcc tcgcgcagca gcgccggcat cagccggtcg agccgctcgc ggccgtgcgc
     1021 gctcatcgag cggtacgcga gactgccccg cagccggtcc agcgcttcga gcgcggccgg
     1081 cggatggtcg tagccggcgg cggcgagccg tgcggcggcg gcctcgcggc tgaccgtctc
     1141 gtgccagacc gccgccagcg cggccatgcc ctcggcgacc ggcgcctcgc gcccgagcgc
     1201 gaacacctgc gcgaagcgtt cggcgacgaa gctgcggtgc gcatcgagcg tctggcggaa
     1261 ggccggccag tcggcaaagc ccatcgagaa cgccagccgc agccggccgg tgtcgtcctc
     1321 gggcaggcgg tgggtctggc ggtcctgcca ggcctgcagg cggttctcgg tgcggcgcag
     1381 gcagtcgtag gcatgctgca ggcgatcgac ggcgtcctcg ggcagccggc ggctgtcggc
     1441 gagccgctca agcacctcgc ggatgccgcg cacgcgcagc ggcggctcgc gtccgccgta
     1501 gatcagctgg aaggcctgac cgatgaactc gatctcgcgg atgccgccgg ggccgagctt
     1561 gacgttgtcc tgcaggccct tgcgctcgac ctcctgcgcg atcatcgcct tgaggttgcg
     1621 caggctctcg aaggcgccgt aatcgagata acggcggaac acgaagggcc gcagcgtctc
     1681 cagcaggcgc tcgccggctt cgaggtcgcc ggctatcgcc cgcgccttga tcatcgcgta
     1741 gcgctcccag tcgcggccct gcgactggta gtaggtttcg agcgccgcga agctcgccac
     1801 cagcggcccg gaatcgccga acggccgcag gcgcatgtcg acgcggaaca cgaagccgtc
     1861 ggcggtcagc gcatcgagca cgcggatcag cttctgcgcc agacgcagga agaactcctg
     1921 gttctccagc ggccgcgggc cggcggtgct gccgtgctcg gggaaggcaa agatcaggtc
     1981 gatgtcggac gaatagttca gctcgcgccc gccgagcttg cccatgccga gcacgaccag
     2041 ccgctgcgcg cgcccgcgct cgtcgcgcgg ctcgccgtgg cgccggctcg ccgcgtcgta
     2101 cagccaggtc agcgcggcat cgacgcaggc atcggcgagc gcgctcaggt cttcgagggt
     2161 ttcggtgagc ttcgccgcgc cggacagatc ccgccaggcg atgcgcacca tctcgcgccg
     2221 gcgcagcgtg cgcaggcgcg ccgacagcac ggcctcatcg gaacaatccg cgagcgccgc
     2281 agcgacgcgc ccgccgatcg cgccgggcac gtcgggcgcc gcgagcgcct ccccggcgat
     2341 cagcccggcg agcaggcgcg gcgtgcgcgt gcagttttcg gcgacgaagt cgctgcaggc
     2401 ccagacccgg accagcgcgg cgtgccagcc ggcggcaacc ggttcgatgc cgtcgcgtgc
     2461 gcaggcctcg cagtagcgct gccagtgcag ggccacgccc ggacgcagcg gctgcggcac
     2521 ggtggccagt acggaatcga gttcatgcat ggtggtgcgc ctcctgccgg cgtctgcacc
     2581 gaaggggtgc atcggcgcag caggctccag cgtagccgcc ggtgcgcgcg ctggcagccg
     2641 gcgcgtggcc gccggctgac gcaggtcagt gcagggtggc cgggctcact cgccgccgag
     2701 catctgcgcg accggcagct ccacgccgtt cagcgtcagc ttgcgctcgc ggtagtcgat
     2761 gacggtgctc aggcgcccgt cggcacgctt gagccagccc tgcgcgagca gctgcgcgag
     2821 gttttcgtcg tagacctgcc gggcgcgctc ggcgacctgc tgcgggtcgg gttcgggcag
     2881 cggcgtgtcg ggctcggtgc cagcctccgg gcggtccgga tcggcctggg caccggcctc
     2941 ctcctgctgc atgcggcgct ccatctcggc catttcggcc tcgttggcca tggccgtgtg
     3001 cacctgctcg gtaacgaggg ccagcacgaa ggactccggc accgccacct gcccctgcgc
     3061 ggcctgcagg tactgcagcg cggcatccgg cgccgacgga tcggtcgggg gctgcagcgt
     3121 cagggccagc cgcgcatcga gcggcagcgg ctcgccggtc ttctcgatgt gcacgttctc
     3181 gaaggcaagc accgggtggt gaggcaggaa ttccatc
//
LOCUS       DAOQJH010000306         3207 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_19759_length_3207_cov_4.199239, whole genome shotgun sequence.
ACCESSION   DAOQJH010000306 DAOQJH010000000
VERSION     DAOQJH010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3207)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3207)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3207
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19759_length_3207_cov_4.199239"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1092)
                     /locus_tag="PLQ18_07795"
     CDS             complement(<1..1092)
                     /locus_tag="PLQ18_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HNE01592.1"
                     /translation="MPELARLPGRLLIVGFGSIGQGVLPLILRHLGVRAADIRIVSAT
                     DDGRAQAEAAGAGFCRCELNPDNLRSVLEPLLAAGDFLLNLSVAVSSLALVRLCRARG
                     ALYLDTCIEPWAGGYADPTLNPAQRSNYALREAALALRAETGPGPTAVLTHGANPGLV
                     SHFTKHALLDLARDTGRPAAPPVSRADWARLAQRLGVRVIHIAERDTQQPTTPKRPGE
                     FVNTWSIDGFAGEGLQPAELGWGSHERRLPPDGHRHARGCDAAIWLARPGASVRVRSW
                     TPLAGPYHGYLITHGESISLADALTVRGPGGSVSYRPTVHYAYHPCDAALLSLHELAG
                     SNWRLQPAQRLIVDEITGGRDELGVLLLGH"
     gene            1310..2809
                     /locus_tag="PLQ18_07800"
     CDS             1310..2809
                     /locus_tag="PLQ18_07800"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNE01593.1"
                     /translation="MLSAPTAAPERSPRLLAAITLFVLFDLLVLAVNFWITWQVEHDA
                     LAINVAGRQRMLTQRIAKSLLLLGRPEDATQARRDLRAAKELFGETLAAFEAGGHVRD
                     GDGVEQPFRALEGSDARALVGEARALWQPLAAQIEAALAGAPGALEPAQRAAVRDTEM
                     LLVRMNRLTGVMQAQSNASTVRLRLLQAVVVGLALLNFALIVSLFRQQLRAAAQAGQA
                     LAELIDHLQDGVAVLDGERRIVMANAALGRLFGVEVGRLTGRPVGELLSHEQERDALL
                     GLRPDGARFAAQCTEVDISLHGRPLTLLTIADVTAEQERQAQLRRMAFQDPLTSVPNR
                     LLALDRLESALLRSLRARRRCAVLFIDLDGFKPINDHYGHATGDLVLIHVARVLQAQV
                     RAADTVARYGGDEFLVILPDLQVADDAMTVAAKISAALHGPVRIGPYELPLGVSIGIA
                     SCPEHAATPEALIAAADAAMYRAKRAGGGLIERARPATQSVTDRRSQPG"
     gene            3076..>3207
                     /locus_tag="PLQ18_07805"
     CDS             3076..>3207
                     /locus_tag="PLQ18_07805"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015781269.1"
                     /note="heat shock protein involved in degradation of
                     misfolded proteins; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HNE01594.1"
                     /translation="MEQFRGTTILAVRRNGRIAIGGDGQVTLGNTVMKGNARKVRRLY
                     "
BASE COUNT          442 a         1136 c         1153 g          476 t
ORIGIN      
        1 gtggccgagc agcagcacgc cgagttcgtc gcgcccgccg gtgatctcgt cgacgatcag
       61 ccgctgcgcc ggctgcagcc gccagttgct gccggcaagc tcgtgcagcg acagcagcgc
      121 ggcatcgcag gggtgatagg cgtaatgcac ggtcggccgg tagctgacgc tgcctccggg
      181 gccgcgcacg gtcagcgcat cggcgagcga gatcgattcg ccgtgcgtga tcaggtagcc
      241 gtgatacggg ccggcgagcg gcgtccagct gcgcacgcgc acgctcgccc cgggtcgcgc
      301 cagccagatc gccgcatcgc agccgcgcgc gtgccggtgg ccgtcgggcg gcagccggcg
      361 ctcgtggctg ccccagccga gttcggccgg ctgcaggccc tcgccggcaa aaccgtcgat
      421 cgaccaggtg ttgacgaact cgccggggcg tttcggggtc gtcggctgct gtgtgtcgcg
      481 ttcggcgatg tggatcacgc gcacgccgag gcgctgcgcg agccgcgccc agtcggcgcg
      541 cgagaccggc ggtgcggccg gccggccggt atcgcgggcc agatcgagca gcgcgtgctt
      601 ggtgaaatga ctgaccagcc ccggattggc gccgtgcgtc agcaccgcgg tcggaccggg
      661 accggtctcg gcgcgcagcg ccagggccgc ctcgcgcagc gcgtagttgc tgcgctgggc
      721 cgggttcagg gtcgggtcgg cgtagccgcc ggcccagggt tcgatgcagg tgtcgagata
      781 cagcgcgccg cgtgcgcggc agagtcgcac cagcgccagc gacgatacgg ccaccgacag
      841 gttcagcagg aagtcgcccg ctgccagcag cggttcgagg acgctgcgca ggttgtcggg
      901 gttcagttcg cagcggcaga agccggcgcc ggcggcctcg gcctgggcgc ggccgtcgtc
      961 ggtggcgctg acgatgcgga tgtcggccgc acgcacgccg aggtggcgca ggatcagcgg
     1021 caggacgccc tggcctatgc tgccgaagcc gacgatcagc aggcgtccgg gcagacgtgc
     1081 aagttccggc atctggaatt ccacgtattt cgcagggtcg atggcgggag catgaccaat
     1141 ccgccgcagc cccgccaccc cggcgagtcc ctgaccgagt ccctgaccaa agcgtatgct
     1201 gcggtgcagc acgattatcg ttacagtgga ttcatataga agcagccaca ctgccagcac
     1261 cggcagccga tgccggttgt ccccgcgtca tccggagtga cgatcgacca tgctgagcgc
     1321 accgaccgcc gcgcccgaac gctcgccgcg cctgctcgcc gccatcacgc tgttcgtgct
     1381 gttcgacctg ctggtgctcg cggtcaactt ctggatcacc tggcaggtcg agcacgatgc
     1441 cctggccatc aacgtcgccg gccgccagcg catgctgacg cagcgcatcg ccaaatcgct
     1501 gctgctgctc ggccggcccg aggatgccac ccaggcccgt cgcgacctgc gcgccgccaa
     1561 ggagctgttc ggcgagacgc tcgcggcctt cgaagccggc ggccacgtgc gcgatggcga
     1621 tggcgtcgaa cagccgttcc gggcgctgga gggcagcgac gcgcgcgcgc tggtcggcga
     1681 ggcccgggcg ctgtggcagc cgctggccgc gcagatcgag gcggcgctcg ccggtgcgcc
     1741 gggagcactg gagccggcgc agcgcgccgc cgtgcgcgat accgagatgc tgctcgtgcg
     1801 catgaaccgc ctgaccggcg tcatgcaggc gcagtcgaat gcgagcacgg tgcggctgcg
     1861 cctgctgcag gccgtggtcg tcgggctggc gctgctgaac ttcgcgctga tcgtctcgct
     1921 gttccgccag cagctgcggg ccgcggcgca ggccggccag gcgctggccg agctgatcga
     1981 ccatctgcag gatggcgttg cggtgctcga cggcgagcgg cgcatcgtca tggccaatgc
     2041 cgcgctcggg cggctgttcg gcgtcgaggt cgggcgactg accggccgcc cggtcggcga
     2101 gctgctgagc catgagcagg agcgcgatgc gctgctcggc ctgcgccccg atggcgcacg
     2161 cttcgcggca cagtgcaccg aggtcgacat cagcctgcac ggccggccgc tgacgctgct
     2221 gaccatcgcc gatgtgaccg ccgagcagga gcgccaggcg cagctgcggc ggatggcttt
     2281 ccaggacccg ctgaccagcg tgccgaaccg gctgctggcg ctcgaccgcc tcgaatcggc
     2341 gctgctgcgc agcctgcgcg cgcgccgccg ctgcgcggtg ctgttcatcg acctcgacgg
     2401 cttcaagccg atcaacgacc actacggtca tgcgaccggc gatctggtgc tgatccacgt
     2461 cgcgcgcgtg ctgcaggcgc aggtgcgggc cgcggacacg gtcgcgcgct atggcggcga
     2521 cgagttcctc gtgatcctgc ccgacctgca ggtcgccgat gacgcgatga ccgtggccgc
     2581 gaagatcagt gcggcgctgc acggcccggt gcgcatcggt ccgtacgagt tgccgctcgg
     2641 cgtcagcatc ggcatcgcca gctgcccgga gcacgcggcc acgcccgagg cgctgatcgc
     2701 cgccgccgac gcggcgatgt accgcgccaa gcgcgccggc ggcggcctga tcgaacgggc
     2761 cagacctgcg acacaatctg tcaccgaccg gcgctcgcag ccgggctgaa cttttccggg
     2821 cctgcaggcg tccatcgtca tcgagcggat gttcttcgct tgcccgatcc cctccctggt
     2881 cgggcggacc cggccccccc tgccgggtcg tcttggagcc ccggaccctc cccctgtacc
     2941 ggggcttttt tatgcacggc tttccgggcg ctcttgaaac cgctgccgca gcgccgcatt
     3001 tcgacggttc gtgtgctgcc gcctcacccg cgcggcagcc gcttcgtcat cagggcagct
     3061 ccggaggcca cttcgatgga acagtttcgc ggcaccacga ttctcgcggt gcgccgcaac
     3121 ggccgcatcg ccatcggcgg tgacggtcag gtcacgctcg gcaacacggt catgaagggc
     3181 aacgcccgca aggtgcgccg gctctat
//
LOCUS       DAOQJH010000307         3196 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_19856_length_3196_cov_4.433938, whole genome shotgun sequence.
ACCESSION   DAOQJH010000307 DAOQJH010000000
VERSION     DAOQJH010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3196
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19856_length_3196_cov_4.433938"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1033
                     /locus_tag="PLQ18_07810"
     CDS             <1..1033
                     /locus_tag="PLQ18_07810"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HNE01595.1"
                     /translation="TASAPVSLPSVPAPRLLRAALPLLIAAPVVLGVQAGGVWVLAQL
                     AKPEPAASAPAATTAAPRTAAPLFASTSSSVPVLAKAPAAEAAAPPAPVISRAPTPEV
                     PAGERPVPAASVEPGAGKPVTAAPAQAPAPGPDAGPVQAASAPEAPVARVEAVAPKPA
                     PASADTADAAPPAVSPVSPTPPAVSPVTRPAASVLYVPPPYVPVAPYAVAAAQAPARI
                     AVTPVAHPAGDKAPAPAPAGTEPERQELLGPDWVRAQPVNNRTLQLMAGSSLEVLREY
                     VRSHDFADVPVAYIPSLRDGQPWYYLIAGSFASVQESSAAERVLKNVQSGLKPWTRRF
                     GGMQALIKP"
     gene            1049..2080
                     /gene="flgJ"
                     /locus_tag="PLQ18_07815"
     CDS             1049..2080
                     /gene="flgJ"
                     /locus_tag="PLQ18_07815"
                     /inference="COORDINATES: protein motif:HMM:TIGR02541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HNE01596.1"
                     /translation="MSTNALSSAAGTPPPVPTALGDFAGLARLRTQSAQQTPEAQREV
                     ARQFEALFIQQMLKTMRAASPGDSLVDSDASRTMRDFHDQKLAESLSQRGDFGIAQLL
                     LKQIAPDAASGRQAGPAIPAAGPAPKLDANALEVLRRVERVRDDGRLEATTAPVASAA
                     YKPGTPEAFVREVWPHAQAAARQLGVAPEVLVAQAALESAWGRSMPTRADGTPSFNLF
                     GIKADRRWQGARVENATTEFVGGRSVREVAGFRAYGSWGESFADYARFIQDNPRYRQA
                     LASAADNSAYLHELHKAGYATDPGYAAKIKRILGGDALNEALRGMGVNTGINTGVNLS
                     AGTAVPGPG"
     gene            2099..>3196
                     /gene="flgK"
                     /locus_tag="PLQ18_07820"
     CDS             2099..>3196
                     /gene="flgK"
                     /locus_tag="PLQ18_07820"
                     /inference="COORDINATES: protein motif:HMM:TIGR02492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HNE01597.1"
                     /translation="MADLLNTGLSSLRAAQLALSTAGHNIANVNTEGYSRQRATLETR
                     LPQYVGEGFIGTGVNVETISRSFDKFLTQQVRSHTATQSQLDVQQGLTEQVDSLLSDG
                     QASLAPLLQGVYDAMQAVAEAPAATAPRQTLLAQAQSLTDRFHEIDSQLGTLRTNVNQ
                     GIRDAVDQINDYAGALAKVNKEIGIASGTGGSSAPPNDLFDERDRLLQKLSEQVGVQA
                     VIAGNGTANVYVGSGQALVTGFDVQKLEVGPDAYTGEDLAVRYAGQRQGTDLAPYLTG
                     GKIGGYIAFRDGMLSRVQGDVGLFAAGIADAVNSQQTRGLDLQGRLGEAMFTTSAARV
                     FAHDSNDGDAQVSVTIARVPDLQRSDYELRYA"
BASE COUNT          475 a         1166 c         1147 g          408 t
ORIGIN      
        1 gactgcttcc gcccccgtct ccctgccgtc cgtccccgct ccccggctgc tgcgcgccgc
       61 gctgccgctg ctgatcgccg cgccggtcgt gctcggcgtg caggccggcg gcgtctgggt
      121 gctcgcgcag ctcgcgaaac ccgagccggc cgccagcgcc ccggccgcca cgacggcggc
      181 gccgcgcacg gcagcgccgc tgttcgcatc cacgtcttca tcggtgccgg tgctcgcgaa
      241 ggcaccggcc gccgaagcgg ctgcgccgcc cgctccggtt atcagccgtg caccgacgcc
      301 ggaagtgccg gccggcgaac gcccggtgcc ggcggcaagc gtggagccgg gcgccggcaa
      361 accggtgact gccgcaccgg cccaggctcc ggctccgggc ccggatgcag ggccggtgca
      421 ggcggcgagc gcgccggaag cgccggtcgc ccgggtggaa gcggtggcgc cgaagccggc
      481 acctgcctcg gcggatacgg cggatgccgc accgccggcc gtgtcgccgg tgtcgccgac
      541 gccgccggcc gtcagtccgg tgacgcggcc ggcggcgagc gtgctgtacg tgccgccacc
      601 ctatgtgccg gtggcgccgt acgcggttgc cgcagcgcag gctccggcgc gcatcgccgt
      661 gacgccggtc gcgcatccgg ccggggacaa ggcccctgcg ccggcgccgg ccgggacgga
      721 gcccgagcgc caggaactgc tcggccctga ctgggtgcgg gcccagccgg tgaacaaccg
      781 cacgctgcag ctgatggcgg gcagttcgct cgaggtgctg cgcgaatacg tgcgcagcca
      841 cgacttcgcc gacgtgccgg tcgcctacat cccgtcgctc cgcgacggcc agccctggta
      901 ctacctgatc gccggcagct ttgcgtcggt gcaggaatcc tcggccgccg agcgggtgct
      961 gaagaacgtg cagtccggcc tgaagccgtg gacgcggcgc ttcggcggca tgcaggccct
     1021 gatcaagccg taagcggagg ccgcagccat gtcgacgaat gcgctcagct ccgccgccgg
     1081 cactccgccg ccggtgccga ccgcgctcgg ggatttcgcc ggactggcgc ggctgcgcac
     1141 gcagagcgcg cagcagacgc cggaagccca gcgcgaggtt gcccgccagt tcgaggcgct
     1201 gttcatccag cagatgctga agaccatgcg ggccgcgagc cccggcgatt cgctggtcga
     1261 cagcgacgcc agccgcacga tgcgcgactt ccatgaccag aagctggccg agagcctgtc
     1321 gcagcgcggc gacttcggca tcgcgcagct gctgctgaag cagatcgcgc cggatgccgc
     1381 gagcggccgt caggccgggc cggcgatccc ggccgccgga ccggcgccga agctcgatgc
     1441 caatgcgctg gaggtgctgc gccgcgtcga gcgcgtgcgc gacgacggca ggctcgaagc
     1501 gacgacggcg ccggtggcga gcgccgccta caagcccggc acgccggagg ccttcgtgcg
     1561 cgaggtctgg ccgcacgcgc aggccgcggc gcgccagctc ggcgtcgcgc ccgaggtgct
     1621 ggtcgcgcag gcggcgctgg aatcggcctg gggccgctcg atgccgacgc gcgccgacgg
     1681 cacgccgagc ttcaacctgt tcggcatcaa ggccgatcgc cgctggcagg gcgcgcgcgt
     1741 cgaaaacgcc acgaccgagt tcgtcggcgg caggtcggtg cgcgaggtcg cgggcttccg
     1801 cgcctatggc tcgtggggcg agagcttcgc ggactacgca cgcttcatcc aggacaaccc
     1861 gcgctaccgg caggccctcg ccagtgccgc cgacaacagc gcctacctgc acgaactgca
     1921 caaggccgga tacgccaccg atccgggcta cgccgccaag atcaagcgca tcctcggcgg
     1981 cgacgcgctg aacgaggcgc tgcgcggcat gggtgtgaac acgggcatca acacgggtgt
     2041 gaacctgtcc gccggcaccg cggtgccggg tccgggctga cgcgagagga gggccgccat
     2101 ggccgatctg ctgaataccg gcctgtccag cctgcgtgcc gcgcagctcg cgctgtcgac
     2161 cgccggccac aacatcgcca acgtcaacac cgagggctac agccgccagc gcgccacgct
     2221 ggaaacccgg ctgccgcagt acgtcgggga aggcttcatc ggcaccggcg tcaacgtcga
     2281 gaccatctcg cgcagcttcg acaagttcct gacccagcag gtgcgctcgc acaccgcgac
     2341 gcagagccag ctcgacgtgc agcagggtct gaccgagcag gtcgacagcc tgctgtccga
     2401 tggtcaggcg agcctcgcgc cgctgctgca gggtgtgtac gacgcgatgc aggcggtcgc
     2461 cgaggccccg gctgcgaccg cgccgcgcca gaccctgctc gcgcaggcgc agtcgctgac
     2521 cgaccgcttc cacgagatcg actcgcagct cgggacgctg cgcaccaacg tcaatcaggg
     2581 catccgcgat gcggtcgacc agatcaacga ctatgccggc gcgctcgcga aggtcaacaa
     2641 ggagatcggc atcgccagcg gcaccggcgg cagttcggcg ccgccgaacg atctgttcga
     2701 tgagcgcgac cggctgctgc agaagctctc cgagcaggtc ggcgtgcagg cggtgatcgc
     2761 cggcaatggc acggccaacg tctatgtcgg ttccggtcag gcgctggtca cgggtttcga
     2821 cgtgcagaag ctggaggtcg ggcccgatgc ctataccggt gaggatctcg cggtgcgcta
     2881 cgccggccag aggcagggca ccgacctcgc gccctatctg accggaggca agatcggcgg
     2941 ctacatcgcc tttcgcgacg gcatgctgag ccgcgtgcag ggcgatgtcg gcctgttcgc
     3001 cgccggcatc gccgatgccg tcaacagcca gcagaccagg ggactcgacc tgcagggccg
     3061 gctcggcgag gcgatgttca cgacctcggc ggcgcgcgtg ttcgcccatg acagcaacga
     3121 cggcgatgcg caggtcagcg tcacgatcgc cagggtcccg gacctgcagc gttcggacta
     3181 cgaactgcgc tacgcc
//
LOCUS       DAOQJH010000308         3195 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_19867_length_3195_cov_3.508917, whole genome shotgun sequence.
ACCESSION   DAOQJH010000308 DAOQJH010000000
VERSION     DAOQJH010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3195
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19867_length_3195_cov_3.508917"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            240..1385
                     /locus_tag="PLQ18_07825"
     CDS             240..1385
                     /locus_tag="PLQ18_07825"
                     /inference="COORDINATES: protein motif:HMM:NF038122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NF038122 family metalloprotease"
                     /protein_id="HNE01598.1"
                     /translation="MSSPFSVRCARSGLVIATLLLPVLPVQALTFNFIADAGMDAQAY
                     DAFVRAGNRWSSVFTDPVTVNLRIGYSALASGVLAQASSTQTIVPYTNFVSALRADRT
                     SSQDTTAVSNLSADLKFLTNRTSDSPNGPGSATPYLDDDGSFNNQYLRMTSANAKALG
                     LMSATATGTDATITFSNAFSYDFKPWDGIVAGTFDFTGLASHEIGHALGFVSGVDILD
                     YYAGHGGPYGEDNFLLDPLDLFRYSVDSAALGVADFTADARAKYLSLNDGRTLLDSLS
                     TGVYYGDGRQDSHWKDNLGIGVMDPTAAAGELLKFGEADLRALDVVGWNRISATGSAA
                     SAPGSAGIAGTAAVPANAVDVDAPATLWLLGSAALLLLARRRTAGAG"
     gene            complement(1387..3024)
                     /locus_tag="PLQ18_07830"
     CDS             complement(1387..3024)
                     /locus_tag="PLQ18_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HNE01599.1"
                     /translation="MTDAAARCPRVSHFREILLWPLQLMPLRADSQIQNHWQVLEQSQ
                     AQHPWREVDDEFTGDPAGFQERHYGEFVTFLPHVRRFLYGDGKGRVSKPAESPIRVFR
                     RNDVKRVRCTFADPQRPVLVLEVAHVDLYFFYDLDVTVLTIELYGSDLDLQVVQDLMF
                     RFGRAYPVYWEADGSGGHCLQRVEWLAADGSVLAASDYEDRGRFLRHVCEYRAPTEAA
                     HWDFILRPLVQHHSDAGGVLRYRQLEYHRMPVMGYLAIDGDPRQLSRNDFMRLTFVTP
                     PGPPERAPYCGAEADGFEARHCYDRFWHTAPDATPGGRYLCCGEAFMMVGSAQSPYFY
                     TPGASLLEQFRHQYFLLFLIPHLHKAALFMMADRLGYWLKQLDIHDPESVKRFKREIR
                     RIKEIFLRFTHRYWFRQLSDQYQARELYRMQREFLGTEALYTEVRERIEDMNIYLDSD
                     SLRRQANTVVRLTVVTAFGLIGTTVTGFFGMNLLGFDGLSPAGKALYFLGVLAATTWV
                     TFYTVMKSKRLSDFLDLLSDEREPFWRKIAALLKVWR"
BASE COUNT          529 a         1045 c         1065 g          556 t
ORIGIN      
        1 catgtccgga ccccgcgcgg ctgcaaggcc gcgcggggtt tttcatgtcc ggtttcatgt
       61 ccggcttgca ggtgatgcgc tgcacaagac atgaacgatt gtaaagaatt tacaaggcac
      121 tgatgggttt gatgtttctg caataattgc ccgaatctgc agggtcgctc atccctgcat
      181 cccgccggca tcgccggtgt ccggtccgat tgcagtgcct tccctccggc aggtgtccca
      241 tgtcttcccc gttttccgtg cgctgcgcac gatccggcct tgtcatcgcc acgctgttgc
      301 tgccggtgct gccggtacag gcgctgacgt tcaacttcat cgccgatgcc ggcatggatg
      361 cgcaggccta cgatgccttc gtgcgtgccg gcaaccgctg gagcagcgtc ttcaccgatc
      421 cggtcacggt caatctgcgc atcggctatt cggcgctcgc cagtggcgtg ctggcccagg
      481 cttcctcgac gcagacgatc gtgccctaca cgaatttcgt cagcgccctg cgggccgatc
      541 gcaccagcag ccaggacacg acggcggtca gcaatctgtc ggccgacctg aaattcctga
      601 ccaaccgtac cagtgacagc ccgaacggtc cgggttcggc gacgccgtat ctcgacgatg
      661 acggttcctt caacaaccag tacctgcgca tgacctcggc caacgccaag gccctgggac
      721 tgatgtcggc gaccgcgacg ggcaccgatg cgacgatcac gttcagcaac gcgttcagtt
      781 acgacttcaa gccctgggac ggcatcgtgg ccgggacttt cgatttcacc ggtctggctt
      841 cccacgagat cggtcatgcg ctcggcttcg tgtcgggcgt cgacatcctc gattattacg
      901 ccggccacgg cgggccgtac ggcgaggaca acttcctgct cgatccgctg gacctgttcc
      961 gctacagcgt cgacagcgcc gcgctcggcg ttgccgattt cacggccgat gcgcgcgcca
     1021 agtatctgtc cctgaacgac gggcgcacgc ttctcgattc cctgtcgacc ggcgtctatt
     1081 acggcgacgg tcgtcaggac agtcactgga aggacaatct cggcatcggc gtcatggatc
     1141 ccaccgccgc cgccggtgaa ctgctcaaat tcggcgaggc agacctgcgg gcgctggatg
     1201 tggtcggctg gaaccggatt tccgcgaccg gcagcgcggc ttcggccccg ggcagtgccg
     1261 gcatcgccgg tacggcggcg gtcccggcca atgccgtcga tgtcgatgcg cccgccacgc
     1321 tgtggctgct cggcagtgca gccctcctgc tgcttgcccg gcgccggacc gcgggagcgg
     1381 gctgattcag cgccagacct tcagcagcgc ggcgatcttg cgccagaacg gctcgcgctc
     1441 gtcggacagc agatcgagaa agtccgacag gcgcttggat ttcatgaccg tgtagaacgt
     1501 cacccaggtc gtcgcggcga gcacgccgag gaaatacagc gccttgccgg ccggcgacag
     1561 tccgtcgaag ccgagcaggt tcatgccgaa gaacccggtc accgtcgtgc cgatcaggcc
     1621 gaaggcggtg accacggtca ggcgcaccac ggtgttggcc tggcggcgca gcgagtcgga
     1681 atcgagatag atgttcatgt cctcgatgcg ctcgcgcacc tcggtgtaga gggcctcggt
     1741 gccgaggaat tcgcgctgca tccggtacag ctcgcgcgcc tggtactggt cggagagctg
     1801 ccggaaccag tagcggtgcg tgaagcgcag gaagatttcc ttgatcctgc ggatctcgcg
     1861 cttgaagcgc ttgaccgatt ccgggtcgtg gatgtcgagc tgcttcagcc agtagccgag
     1921 ccggtccgcc atcatgaaca gcgccgcctt gtgcaggtgc gggatcagga acagcaggaa
     1981 gtactgatgg cggaactgct ccagcaggct cgcgcccggc gtgtagaagt acggcgactg
     2041 cgcgctgccg accatcatga aagcctcgcc gcagcagagg tagcgcccgc ccggggtggc
     2101 gtccggtgcc gtgtgccaga agcggtcgta acagtggcgg gcctcgaagc catcggcctc
     2161 ggccccgcag tacggcgcgc gttccggcgg gccgggcggg gtcacgaagg tcaggcgcat
     2221 gaagtcgttg cggctcagct ggcgcggatc gccgtcgatc gccagataac ccatgaccgg
     2281 catgcggtgg tattcgagct ggcggtagcg cagcacgccg cctgcgtccg agtgatgctg
     2341 caccagcggc cgcaggatga aatcccagtg cgcggcctcg gtcggcgcgc ggtactcgca
     2401 gacgtggcgc aggaatctgc cgcggtcctc gtaatcggag gctgcgagca cgctgccatc
     2461 ggcggccagc cattcgacgc gctgcaggca gtggccgccg ctgccgtcgg cctcccagta
     2521 gaccggatag gcgcggccga agcggaacat caggtcctgc acgacctgca ggtcgagatc
     2581 cgagccgtac agctcgatcg tcagcaccgt cacatcgagg tcatagaaga agtacaggtc
     2641 gacgtgtgcg acctccagca ccagcaccgg gcgctgcgga tcggcgaagg tgcagcgcac
     2701 gcgcttcacg tcgttgcgcc ggaacacgcg gatcggagat tccgccggct tcgacacccg
     2761 gcccttgccg tcgccgtaca ggaagcggcg cacgtgcggc aggaaggtca cgaactcgcc
     2821 gtagtggcgc tcctggaagc cggccggatc gccggtgaac tcgtcgtcga cctcgcgcca
     2881 cggatgctgc gcctgcgact gttcgagcac ctgccagtgg ttctggatct ggctgtcggc
     2941 gcgcagcggc atcagctgca gcggccagag caggatctcg cggaaatggc tcacgcgcgg
     3001 gcagcgggca gcagcatcgg tcatggcgga cggcactcgg gcgaggggct gcgagtatag
     3061 gcaggcgcag cgcaagggcc cgcgcgccgg gcgtagcgga taccatgggt gactgtgtca
     3121 ttccgtttcc gcgccggagt ccgtcctctt gcccccgaat ttgaccgctg ccgcgctgcg
     3181 tcgcggccgg gcgat
//
LOCUS       DAOQJH010000309         3187 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_19943_length_3187_cov_4.672414, whole genome shotgun sequence.
ACCESSION   DAOQJH010000309 DAOQJH010000000
VERSION     DAOQJH010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3187
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19943_length_3187_cov_4.672414"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(12..2819)
                     /locus_tag="PLQ18_07835"
     CDS             complement(12..2819)
                     /locus_tag="PLQ18_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HNE01600.1"
                     /translation="MLSDPLILGSALFYIGLLFAVAYWGDRAAEAGRSVIANPWTYTL
                     SLGVYCTAWTFYGSAGRAAHGGIAFLPVYLGPTLVLVLGWQLVRRIVRISQRNRITSI
                     ADLIGSRYGKSHLLAGLVTVIAVIGIMPYISIQLKAIATSYTVLRGYPEVSPLVRVAE
                     QPVWADTTLYAALALIAFAVLFGTRHIDNTERHEGLVAAIAFESVVKLGAFLLVGAYV
                     VWSLNDGFGDIFTRAAAHPELAKLMRFDAIGGGYAGWVTLTLLSACAFLFLPRQFQVL
                     VVENTDEHHLRTASWAFPLYLWLINLFVLPVALAGLMTFESRGLDADLFVLDLPIHGR
                     EPFLAVLVFVGGLSAATSMVIVETVALSTMVCNDLVMPLLLRRLRPGGRRDLSGLLLG
                     IRRIAIAGIVLLGYLYYRVIGESYTLVSIGLVSFAACAQFAPVILAGIYWKGATRRGA
                     YWALSAGFAVWAYTLLLPSFARSGWLPEGFVTDGPFGIELLKPYALFGLAGLDQYSHA
                     LVWSWLANVGGLLLGSLWDRPGALERVQASQFVDAWDSSSTARLLWRGTADIHELQTL
                     VARFLGPERAASAFAGYAQSRGLEPGRHGRAEAELVHFAEHLLAGAIGAASARVTVSS
                     VVKGEALSIEEVMTILDETSQAIQYSRRLEEQSRELEAAGRELRAANARLQELDRLKD
                     EFVSTVTHELRTPLTSIRAFAEILLGAPDIPDTQRAEFLGIIVKESERLSRLINQVLD
                     MAKLDSGRVDWRSEPVELRALLRDAANATSQLFRDKGAELTLDLDEAPAAGADTADGS
                     LAGAHALHLAQQPAVITGDSDRLQQVLINLLSNAVKFCPEVHGRVAVRLRVRENGVRI
                     EVADNGPGIPLAEQARIFEKFHQVSSQQAGKPKGTGLGLTISQRIVERHGGTIRVESI
                     EGEGACFIVELPAQVLANAAA"
     gene            complement(2877..>3187)
                     /locus_tag="PLQ18_07840"
     CDS             complement(2877..>3187)
                     /locus_tag="PLQ18_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020505563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HNE01601.1"
                     /translation="ACTSFQVVQECLNTMLRKASIPLDTAGMRDYLDIVLLPLVRVPA
                     SADLYRRSLEVRERYGFSFYDALIVATALEAGCTRLWSEDLQHGQRIGVLQVENPFRA
                     "
BASE COUNT          519 a         1096 c         1146 g          426 t
ORIGIN      
        1 tcaggcggtg ctcaggccgc cgcgttcgcc agcacctgcg ccggcagctc gacgatgaag
       61 caggcacctt cgccctcgat gctctcgacg cggatcgtgc cgccgtggcg ctcgacgatg
      121 cgctggctga tggtcagccc gaggccggtg cccttgggct tgccggcctg ctggctgctg
      181 acctggtgga acttctcgaa gatgcgcgcc tgctcggcga gcgggatgcc gggaccgttg
      241 tcggcgacct cgatgcgcac gccgttctcc cgcacgcgca ggcgcaccgc gacccggccg
      301 tggacttcgg ggcagaactt cacggcgttc gacagcaggt tgatcagcac ctgctgcagc
      361 cggtcgctgt cgccggtgat cacggccggc tgctgcgcga ggtgcagcgc gtgcgcaccg
      421 gcgaggctgc cgtcggccgt atccgcaccg gcggccgggg cctcgtcgag atcgagcgtc
      481 agctccgcgc ccttgtcacg gaacagctgg ctggtggcgt tggcggcatc gcgcagcagc
      541 gcgcgcagct cgaccggctc gctgcgccag tcgacgcgcc cggaatcgag cttggccatg
      601 tcgagcacct ggttgatcag ccggctcagg cgctcgctct ccttgacgat gatgccgagg
      661 aactcggcgc gctgcgtatc ggggatgtcg ggggcgccga gcaggatctc ggcgaaggcg
      721 cggatcgagg tcagcggcgt gcgcagctcg tgcgtgaccg tcgacacgaa ttcgtccttg
      781 agccggtcga gctcctgcag ccgggcgttg gcggcgcgca gctcccggcc ggcggcttcg
      841 agttcgcgcg actgctcttc gagccggcgg ctgtactgga tggcctgcga ggtctcgtcg
      901 aggatggtca tgacctcctc gatcgacagc gcctcgccct tgaccaccga ggacaccgtc
      961 acccgcgccg aggccgcgcc gatcgcgccg gcgagcaggt gctcggcgaa gtgcacgagc
     1021 tcggcctcgg cgcggccgtg gcggcccggt tcgaggccgc ggctctgcgc gtagccggcg
     1081 aaggcgctgg cggcgcgctc cggaccgagg aagcgcgcga ccagcgtctg cagctcgtgg
     1141 atgtcggcgg tgccgcgcca cagcagccgg gcagtgctgc tgctgtccca ggcatcgacg
     1201 aactggctgg cctgcacgcg ctccagcgca ccggggcggt cccacagcga gccgagcagc
     1261 aggccgccga cgttggcgag ccagctccag accagcgcgt gcgagtactg gtcgagcccg
     1321 gcgaggccga acagcgcgta gggtttcagc agctcgatgc cgaacggccc gtcggtgacg
     1381 aagccctcgg gcagccagcc cgagcgcgcg aacgacggca gcagcagcgt gtacgcccag
     1441 accgcgaagc cggccgacag cgcccagtag gcaccgcggc gcgtcgcgcc cttccagtag
     1501 atgccggcga ggatcaccgg cgcgaactgc gcgcaggccg cgaacgacac gaggccgatc
     1561 gacaccagcg tgtaggactc gccgatcacc cggtagtaca ggtagccgag cagcacgatg
     1621 ccggcgatgg cgatgcggcg gatgccgagc agcagcccgg agaggtcgcg ccgcccgccc
     1681 gggcgcagcc ggcgcagcag cagcggcatc accaggtcgt tgcagaccat cgtcgacagc
     1741 gccacggtct cgacgatgac catgctggtc gccgccgaca gcccgccgac gaacaccagc
     1801 acggcgagga agggctcgcg gccgtggatc ggcaggtcga gcacgaacag gtcggcatcg
     1861 aggccgcggc tctcgaaggt catcagcccg gcgagcgcca ccggcagcac gaacaggttg
     1921 atcagccaca gatacagcgg gaaggcccag ctcgcggtgc gcaggtggtg ctcgtcggtg
     1981 ttctcgacca ccagcacctg gaactggcgc ggcaggaata aaaaggcgca ggccgacagc
     2041 agcgtcagcg tcacccagcc ggcgtagccg ccgccgatcg catcgaagcg catcagcttc
     2101 gcgagttccg ggtgcgcggc ggcgcgcgtg aagatgtcgc cgaagccgtc gttgaggctc
     2161 cagaccacgt aggccccgac cagcaggaag gcgccgagct tgaccaccga ctcgaacgcg
     2221 atcgccgcga ccaggccctc gtggcgttcg gtgttgtcga tgtggcgggt gccgaacagc
     2281 accgcgaacg cgatcagcgc cagcgccgcg tacagcgtgg tgtcggccca caccggctgc
     2341 tcggcgacgc gcaccagcgg cgagacttcc ggatagccgc gcagcaccgt gtagctggtg
     2401 gcgatcgcct tcagctggat ggagatgtac ggcatgatcc cgatcaccgc gatcaccgtc
     2461 accagcccgg cgagcaggtg gctcttgccg tagcgcgagc cgatcaggtc ggcgatcgac
     2521 gtgatgcggt tgcgctggct gatgcgcacg atgcgccgca ccagctgcca gccgagcacc
     2581 agcaccagcg tcggccccag gtacaccggc aggaaggcaa tcccgccgtg cgcggcgcgc
     2641 ccggcgctgc cgtagaaggt ccaggccgtg cagtagacgc cgagcgacag cgtgtacgtc
     2701 cacggattgg cgatcaccga gcgcccggct tcggcggcac gatcgcccca gtacgcgacc
     2761 gcgaacagca agccgatgta gaacagcgca ctgccgagga tcagcggatc ggagagcatc
     2821 gcggcacctt cggccgggcg gaacgggatc gggatcggga gtgccggagc agacgctcag
     2881 gcgcggaacg ggttttcgac ctgcagcaca ccgatgcgct ggccgtgctg caggtcctcg
     2941 ctccagagcc gggtgcagcc ggcttcgagc gccgtggcga cgatcagcgc gtcgtagaag
     3001 ctgaagccgt agcgctcgcg gacctcgagg ctgcgccggt acagatcggc gctcgccggc
     3061 acccgcacca gcggcagcag cacgatgtcg agatagtcac gcatgcccgc cgtgtcgagc
     3121 gggatcgagg ccttgcgcag catcgtgttg aggcattcct gcacgacctg gaagctggtg
     3181 caggcac
//
LOCUS       DAOQJH010000310         3187 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_19944_length_3187_cov_4.228927, whole genome shotgun sequence.
ACCESSION   DAOQJH010000310 DAOQJH010000000
VERSION     DAOQJH010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3187
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19944_length_3187_cov_4.228927"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(123..2297)
                     /gene="tssI"
                     /locus_tag="PLQ18_07845"
     CDS             complement(123..2297)
                     /gene="tssI"
                     /locus_tag="PLQ18_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011979145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
                     /protein_id="HNE01602.1"
                     /translation="MPITQANRPVTVSCSLGTDKLLLRRLHGRERLGRLFEYELELAS
                     EDFAIDPAGLLGKPLGVELELPSGDGSRWFHGIVASFGQSGSDGRIATYRVRLRPWFW
                     LLTHCSDCRIFQAKSVPDIVKQVFENLGFADYELKLNGSYAPREYCVQYRETDFDFVS
                     RLLEKEGIFYYFRHEDGRHVLVLVDDNASLAAQPGYEEVPYFPPDQLGRRPRDHLWAW
                     SQALSVQTSALTLNDFDFERPSAQLLAASSASGPAAGYKRYDYPGAYTQTSPGEHYAR
                     TQLEALRTRAAECRFTGDARGLSCGALFTLSGCPNDKLNIELLITSAEYRLTQDAYHS
                     GPNAGKLDFELDIAAQDNQLPFRSEPLTPPAQVLGPQTAIVVGPAGEEIFTDKYGRVK
                     VHFHWDRYDGQDEHSSCWIRVAQVWAGKGWGGMMIPRIGQEVVVDFLEGDPDRPLITG
                     RVYNGEQPVPFALPANATQSGWRSRSSKGGSTANCNEIRMEDLKGSEELFIHAEKDEL
                     HEVENDQTLSVGHDRSETTGRDRTLVVGRDKSETVDRHKTVHIVGNHVERIDMNMEVS
                     VASNLTETVGINYSETVGAAMELTVGGLLNIAVGAAMSEEVGLQKTDVVGGAHSVTIG
                     GNRSIEVGGAVTETIDKDQTNKIGKNLTTEVGENCKETVKKDYSLQAKRIQLVADDEI
                     ALKTGSAELTLKKDGTILIKGAKITIKGSGDVVIKGSKIAEN"
     gene            complement(2319..>3187)
                     /locus_tag="PLQ18_07850"
     CDS             complement(2319..>3187)
                     /locus_tag="PLQ18_07850"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNE01603.1"
                     /translation="CHEYEAAAVHHRAALALDPQHLGATRALGHVLSRLGRLDEAAAA
                     YRAWLALEPGHPLAELRLAACGGAPAPARCSDAVVRGLFDQYAGHFDAHLRGTLDYHA
                     PERLADALALVLDAPAAALDVLDAGCGTGLCGPLLRPYARQLSGVDLSPKMLEQAAAL
                     ALYDALETAELCAWLNAHPGAFDLLVAADTLCYFGPLADFAAAAAAALRPGGWLAFTL
                     ETHTEALDHRLNACGRYSHAPDYPLRVLAGAGFGQLRSQPVVLRRERGAPVEGLVVLA
                     RRPATPGYACAD"
BASE COUNT          440 a         1077 c         1141 g          529 t
ORIGIN      
        1 gggccagtcg accagcggcc ggccctcggc gtcgatgccg ctccagagcc cctcggtgcc
       61 gccgctgccc gccggagccg gcgcgggcgc tgccggaatc cgcagcgtga tctgcttcat
      121 gctcagttct ccgcgatctt gctgcccttg atgaccacgt cgccgctgcc cttgatcgtg
      181 atcttcgcgc ccttgatcag gatggtgccg tccttcttca gcgtcagctc ggccgagccg
      241 gtcttcagcg cgatctcgtc atcggcgacc agctggatgc gcttggcctg cagcgagtag
      301 tccttcttga cggtttcctt gcagttctcg ccgacctcgg tggtcaggtt cttgccgatc
      361 ttgttggtct ggtccttgtc gatggtctcg gtgacggcgc cgccgacctc gatcgagcgg
      421 ttgccgccga tcgtcaccga atgcgcgccg ccgaccacgt cggtcttctg caggccgacc
      481 tcctcgctca tcgccgcgcc gacggcgatg ttcagcaggc cgccgacggt cagctccatc
      541 gccgcaccga cggtctcgga ataattgatg ccgacggtct cggtcaggtt cgaggcgacc
      601 gagacctcca tgttcatgtc gatgcgctcg acgtggttgc cgacgatgtg cacggtcttg
      661 tgccggtcga cggtctcgga cttgtcgcgc ccgaccacca gcgtgcggtc gcgcccggtg
      721 gtctcggagc ggtcgtggcc gaccgacagg gtctggtcgt tctcgacctc gtgcagttcg
      781 tccttctcgg cgtggatgaa gagttcctcg gagcccttga gatcctccat gcggatctcg
      841 ttgcagttcg ccgtcgagcc gcccttgctg gagcggctgc gccagccgct ctgcgtggcg
      901 ttggccggca gcgcgaacgg caccggctgc tcgccgttgt agacgcggcc ggtgatcagc
      961 ggccggtccg gatcgccttc gaggaagtcg acgacgacct cctggccgat gcgcggaatc
     1021 atcatcccgc cccagccctt gccggcccag acctgcgcga cgcggatcca gcacgagctg
     1081 tgctcgtcct ggccgtcgta gcggtcccag tggaagtgca ccttcacgcg cccgtacttg
     1141 tccgtgaaga tttcctcgcc ggccggaccg acgacgatcg cggtctgcgg cccgagcacc
     1201 tgtgccggcg gcgtcagcgg ctcgctgcga aacggcagct ggttgtcctg cgcggcgatg
     1261 tcgagttcga agtcgagttt cccggcgttc gggccgctgt ggtaggcgtc ctgcgtcagc
     1321 cggtattcgg ccgacgtgat cagcagttcg atgttgagct tgtcgttcgg gcagccgctc
     1381 agcgtgaaca gcgcaccgca cgacaggccg cgggcatcgc cggtgaagcg gcattcggca
     1441 gcgcgcgtgc gcagggcttc gagctgcgtg cgcgcgtagt gctcgccggg gctggtctgc
     1501 gtgtaggcgc cggggtagtc gtagcgcttg tagccggcgg ccggaccgct cgcgctcgag
     1561 gctgccagca gctgcgccga cgggcgctcg aagtcgaagt cgttcagcgt cagtgccgag
     1621 gtctgcacgc tcagcgcctg cgaccacgcc cacaggtgat cgcgcggccg gcgcccgagc
     1681 tggtcgggcg ggaagtacgg cacttcctcg tagccgggct gcgcggcgag gctggcgttg
     1741 tcatcgacga gcaccagcac atggcgcccg tcctcgtggc ggaagtaata gaagatgcct
     1801 tccttctcca gcaggcggct gacgaagtcg aaatcggtct cgcggtactg cacgcagtat
     1861 tcgcgcggcg cgtagctgcc gttgagcttc agttcgtagt cggcaaagcc gaggttctcg
     1921 aacacctgct tgacgatgtc cggcacgctc ttcgcctgga agatgcggca gtccgagcag
     1981 tgcgtcagca gccagaacca cggccgcagc cgcacgcggt aggtggcgat gcgcccgtcg
     2041 ctgccgctct gcccgaagct cgcgacgatg ccgtggaacc agcgcgagcc gtcgccggac
     2101 ggcagttcga gctcgacgcc gagcggcttg ccgagcagcc cggccgggtc gatcgcgaag
     2161 tcctcggagg cgagttcgag ttcgtattcg aacaggcgcc cgagccgctc gcggccgtgc
     2221 agccggcgca gcagcagctt gtcggtaccg aggctgcagc tgaccgtgac cggtcggttc
     2281 gcctgcgtga tcggcatcgg gggtctccgg ggatgcgttc agtccgcgca agcatagccg
     2341 ggcgtcgccg gccggcgcgc gagcacgacc agaccctcga ccggcgcgcc gcgctcgcga
     2401 cgcagcacga ccggctggct gcgcagctgc ccgaagccgg ccccggcgag cacgcgcagc
     2461 ggataatccg gggcgtggct gtagcggccg caggcattga gccggtgatc aagggcttcg
     2521 gtgtgggttt cgagcgtgaa cgccagccag ccgcccgggc gcagcgcggc agcggcagcg
     2581 gcggcaaagt ccgcgagcgg cccgaagtaa cagagtgtgt cggcggcgac cagcaggtcg
     2641 aacgcgccgg gatgagcatt gagccaggca cacagttcgg cggtttcgag ggcgtcgtac
     2701 agcgccagcg ccgccgcctg ttcgagcatc ttcggtgaca ggtcgacgcc gctcagctgc
     2761 cgtgcatacg ggcgcagcag cgggccgcac aggccggtgc cgcagccggc atcgagcacg
     2821 tcgagcgcgg cggccggggc gtcgagcacc agcgccagcg catcggcgag gcgctcgggc
     2881 gcgtggtaat cgagcgtccc gcgcaggtgc gcgtcgaaat ggccggcgta ctggtcgaac
     2941 agcccgcgca cgacggcatc gctgcagcgc gccggcgccg gtgcgccgcc gcaggcggca
     3001 aggcgcagtt cggcgagcgg atggccgggt tcgagcgcga gccaggcgcg gtaggccgcg
     3061 gcggcctcgt cgagccggcc gaggcgcgac agcacatggc cgagcgcgcg cgtggcaccg
     3121 aggtgctgcg gatcgagcgc gagcgccgcg cggtgatgca cggccgcggc ctcgtattcg
     3181 tgacagc
//
LOCUS       DAOQJH010000311         3182 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_20000_length_3182_cov_4.037416, whole genome shotgun sequence.
ACCESSION   DAOQJH010000311 DAOQJH010000000
VERSION     DAOQJH010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3182
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20000_length_3182_cov_4.037416"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..787)
                     /locus_tag="PLQ18_07855"
     CDS             complement(<1..787)
                     /locus_tag="PLQ18_07855"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765267.1"
                     /note="catalyzes the conversion of citrate to isocitrate
                     and the conversion of 2-methylaconitate to
                     2-methylisocitrate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase B"
                     /protein_id="HNE01604.1"
                     /translation="MLDAYRKHAEERAAQGIPALPLSAQQTADLVELLKNPPAGEEAF
                     LLDLLTHRVPAGVDQAAYVKAAFLAAVAKGEASSPLLNRVRATELLGTMLGGYNIQPL
                     IDLLDDAECAAAAGKALSHTLLMFDYKNAVKDKADAGNATAAAVLKSWADAEWFTSRP
                     KVPESVTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNPRPGIEADQPGSVG
                     PIKQLAALQAKGNPIAYVGDVVGTGSSRKSATNSVLWWTGEDIP"
     gene            1101..2744
                     /locus_tag="PLQ18_07860"
     CDS             1101..2744
                     /locus_tag="PLQ18_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011354992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid--CoA ligase"
                     /protein_id="HNE01605.1"
                     /translation="MSISDPGRVPAAQAATRLNLGQLLCNASRQFPKQLIVHGERRFT
                     YAEFGRRVHRLAAVLDALGVRAGETVAVLDWDSHRYLEAFLAVPMLGAVLHTVNVRLS
                     PEQIAWTIEHAADDVILVHEDFLPLLDAIRPRLAHVRRFVLLCDHAGGEALGGLPFAG
                     EYEALLAAATGDFAFPEVAEDARATTFYTTGTTGLPKGVFFSHRQLVLHTLGVRSTLS
                     GTGQGHIGADDVYMPITPMFHVHAWGMPLVALSMGLKQVYPGRYEPAKLLRLIVREGV
                     SFSHCVPAILQMLLNCPDGREMDLDGWKVLIGGSALPESLAIAALERGIDVFGGYGMS
                     ETCPILCVAQLTPEVLRRPFAEQGRIRCKAGRAAPLVQLRIVDDEMNDVAHDGIAQGE
                     VIARAPWLTEGYLHNPEAGAALWRGGWLHTGDIGTIDADGYLKITDRLKDVIKTGGEW
                     VSTLMLEDLILRLHEVAECAVVGIPDPRWDERPLALVVLRGGASLDPAMIRAHLAGFV
                     AEGLIPKYALPERIEQVEAIPKTSVGKLDKKAIRAQYAG"
     gene            complement(2766..>3182)
                     /locus_tag="PLQ18_07865"
     CDS             complement(2766..>3182)
                     /locus_tag="PLQ18_07865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE01606.1"
                     /translation="PDPAMAVAVTAALLDAGRVAAAIGHGSALLAGAALLATLPASGT
                     TRLCCGLALALWPVSGWLALRVALDARLFATLDADEASFTRFDACLPALGLYPSQPGP
                     RPLPLRCRGALRLWRSLLAVAGVQFALVLVAALARH"
BASE COUNT          461 a         1064 c         1174 g          483 t
ORIGIN      
        1 aggggatgtc ctcgccggtc caccacagca cggagttggt cgcggacttg cggctggagc
       61 cggtgccgac cacgtcgccg acgtaggcga tcgggttgcc cttggcctgc agcgcggcaa
      121 gctgcttgat cggaccgacc gagccgggct gatcggcttc gatgcccgga cgcgggttct
      181 tcagcatcgc cagcgcgtgc agcgggatgt ccggacgcga ccaggcgtcg ggagccgggg
      241 acaggtcatc ggtgttggtt tcgccggtga ccttgaatac ggtgacggtg acgctctccg
      301 ggaccttcgg gcggctcgtg aaccactcgg cgtcggccca ggacttcagc acggccgcgg
      361 cggtggcgtt gccggcatcg gccttgtcct tgacggcgtt cttgtagtcg aacatcagca
      421 gcgtgtgcga cagcgccttg ccggcggcgg cggcgcattc ggcgtcgtcg agcaggtcga
      481 tcagcggctg gatgttgtag ccgccgagca tggtgcccag cagctcggtg gcgcgcacgc
      541 ggttgagcag cggcgagctc gcctcgccct tggcgacggc ggcgaggaag gcggccttga
      601 cgtaggcggc ctggtcgacg ccggccggca cgcggtgggt cagcaggtcg agcaggaagg
      661 cttcttcgcc tgcgggcgga ttcttcagga gttcgaccag gtccgcggtc tgctgggcgg
      721 acagcggcag ggccggaatg ccctgggcag cgcgttcctc ggcgtgtttg cgataggcat
      781 ctaacacggt ttaaaccccc tcgttgcgtg atgagctgcc ggcgggcagc gggtctggaa
      841 aaccccgatc catgaagcga tgtgaggcgc ggttcgtgct gcgcctcgct ccggctgcgg
      901 ctctgtggcc gcttgtccgg aaaaatcgcg gcagtctaaa cggaatcggc gcacgcggaa
      961 aaccggaagc cgttgcggca tgcgacattg tggacattcc ccgggggctg gatggctgac
     1021 agtgcgcagc gcgcaggcca gactcggggt caaaggaccg gcagcagacc ggccgtcatg
     1081 agcaggggag tctccacacc atgtccattt ccgatcccgg ccgcgtgccg gccgcccagg
     1141 cggcgacgcg cctgaatctc ggccagctgc tctgcaacgc ctcgcgccag ttcccgaagc
     1201 agctcatcgt gcacggcgag cggcgcttca cgtatgccga gttcggccgg cgcgtgcacc
     1261 ggctcgctgc cgtgctcgat gccctcggcg tgcgcgccgg cgagacggtc gcggtgctcg
     1321 actgggattc gcaccgctac ctcgaagcct tcctcgcggt gccgatgctc ggggcggtgc
     1381 tgcacacggt gaacgtgcgc ctgtcgcccg agcagatcgc gtggacgatc gagcatgccg
     1441 cggacgacgt gatcctcgtg catgaggact tcctgccgct gctcgatgcg atccgcccgc
     1501 ggctggcgca cgtgcgccgc ttcgtgctgc tctgcgatca cgccgggggc gaggccctgg
     1561 gcggcctgcc gtttgccggc gagtacgagg cgctgctggc ggcggcgacc ggggactttg
     1621 ccttcccgga ggtggccgag gatgcgcgcg cgacgacctt ctacacgacc ggcaccaccg
     1681 gcctgccgaa gggcgtgttc ttcagccacc gccagctggt gctgcacacg ctcggcgtgc
     1741 gctcgacgct ttccggcacc gggcagggcc acatcggcgc cgacgatgtc tacatgccga
     1801 tcacgccgat gttccacgtg cacgcctggg gcatgccgct ggtggcgctg tcgatggggc
     1861 tgaagcaggt gtatccgggc cgttacgagc cggcgaagct gctcaggctg atcgtgcgcg
     1921 agggcgtgag cttctcgcac tgcgtaccgg ccatcctgca gatgctgctg aactgcccgg
     1981 acggccgcga gatggatctc gacggctgga aggtgctgat cggcggttcg gcgctgccgg
     2041 agtcactggc gatcgcggcg ctggagcgcg gcatcgacgt gttcggcggc tacggcatgt
     2101 ccgagacctg tccgatcctc tgcgttgcgc agctgacgcc cgaggtcctg cgccggccct
     2161 tcgccgaaca ggggcgcatc cgctgcaagg ccgggcgcgc cgcgccgctg gtgcagctgc
     2221 gcatcgtcga tgacgagatg aacgacgtgg cgcacgacgg catcgcgcag ggcgaggtga
     2281 tcgcccgcgc gccgtggctg accgagggct atctgcacaa tcccgaggcc ggcgcggcgc
     2341 tctggcgcgg cggctggctg cacaccggcg acatcggcac gatcgacgcc gatggctacc
     2401 tgaagatcac cgatcgcctc aaggatgtca tcaagaccgg cggcgagtgg gtgtcgacgc
     2461 tgatgctcga agacctgatc ctgcgcctgc acgaggtcgc cgaatgcgcg gtggtcggca
     2521 tccccgatcc gcgctgggac gaacggccgc tggcgctggt cgtgctgcgc ggcggggcca
     2581 gcctggaccc cgcgatgatc cgcgcgcatc tggccggttt cgtcgccgaa gggctgatcc
     2641 cgaagtacgc gctgcccgag cgcatcgaac aggtcgaggc gattccgaag accagcgtcg
     2701 gcaagctcga caagaaggcg atccgcgcgc agtacgccgg ctgagcgcgg ccggccggcc
     2761 gggtttcagt gccgcgcgag ggctgccacc agcaccagcg cgaactgcac gccggccacc
     2821 gccagcaggc tgcgccacag ccgcagcgcg ccgcgacagc gcagcggcag cggtcgcggg
     2881 ccgggctgcg acggatacag gccgagtgcc ggcaggcagg catcgaagcg cgtgaagctc
     2941 gcctcgtcgg catcgagcgt tgcgaacagc cgcgcatcga gcgcgacgcg cagcgccagc
     3001 cagccgctca ccggccacag cgccagcgcc agtccgcagc agagccgcgt cgtgccgctg
     3061 gccggcagcg tggcgagcag cgcggcaccg gcgagcagcg cgctgccgtg gccgatggcg
     3121 gcagcgaccc ggccggcatc gagcagcgcg gcggtgacgg ccacggccat tgccgggtcg
     3181 gg
//
LOCUS       DAOQJH010000312         3178 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_20055_length_3178_cov_4.415946, whole genome shotgun sequence.
ACCESSION   DAOQJH010000312 DAOQJH010000000
VERSION     DAOQJH010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3178)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3178)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3178
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20055_length_3178_cov_4.415946"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1182
                     /locus_tag="PLQ18_07870"
     CDS             <1..1182
                     /locus_tag="PLQ18_07870"
                     /inference="COORDINATES: protein motif:HMM:NF017262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HNE01607.1"
                     /translation="AAAALAAGDIVALALPWLRPEGKARLRSVRVVRVAARLGLALLL
                     PRLLAEGAALAGTSDWFEDETYAALAHTAYPLAAALALPGLLVLACLPKTGEWKRGWL
                     LAGAVPALLSFGVGGHAVHALTGGWLAPVLVVHAAAAAIWLGALWPTEYALRSVDPKI
                     DDQRPALAVLGRFAHRGRELLGALAVSGLLIACVQLGPPSPAWGNTYARLFMAKLGLA
                     LLLFALGALNRWCWLPALARGRRRAASRLRLSVRLCRGLLVAVVALSVALGTTMPPRA
                     GAGAVAPPPAALVERHWPSAYGPRLGLNVAMQAGRPAATLSVTGPDDQPFTIREAVLV
                     LERPEAGIEPLRRRLDERAPGRYGNDDLLLPIAGPWQGYVEIWIDDFSLERQPLAFTL
                     P"
     gene            1292..1957
                     /locus_tag="PLQ18_07875"
     CDS             1292..1957
                     /locus_tag="PLQ18_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012430134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxychromene-2-carboxylate isomerase"
                     /protein_id="HNE01608.1"
                     /translation="MTQPTDAPALPTLDWYFDYLSPFAYFQLERFTAEPPPGVVLRPQ
                     PVLLGALLGACGARGPAEIPLMRTLTYRHCLWQARRPGLPYRMPPAHPFNPLPALRLT
                     VALGAGPDTVRTVFRHLWAEGRTLEGEDWQALCARLGLSVAEAGALAASPAAKDGLRE
                     RTEAAIARGVFGVPCSIVHGELFWGNDTTGMLLDFLADPALFRDAEMQRVSTLPAAIQ
                     RRA"
     gene            1960..2397
                     /locus_tag="PLQ18_07880"
     CDS             1960..2397
                     /locus_tag="PLQ18_07880"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019644625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HNE01609.1"
                     /translation="MARIAHAEFEELCREHVPFVGSLGIRVETLDAGRCVLRVPFRDD
                     FLRPGGTVSGPIQMALADIVMYGVVLSLLGRVDLAVTTNLTCNFLRRPKPVDLIGDGR
                     ILKLGKRLAVGEVLLYSDGETEPVAHVTCTYSIPPGAGQPIAN"
     gene            complement(2626..2699)
                     /locus_tag="PLQ18_07885"
     tRNA            complement(2626..2699)
                     /locus_tag="PLQ18_07885"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2665..2667),aa:Cys,seq:gca)
     gene            complement(2789..2864)
                     /locus_tag="PLQ18_07890"
     tRNA            complement(2789..2864)
                     /locus_tag="PLQ18_07890"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2829..2831),aa:Gly,seq:gcc)
     gene            complement(2917..>3178)
                     /locus_tag="PLQ18_07895"
     CDS             complement(2917..>3178)
                     /locus_tag="PLQ18_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002684813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HNE01610.1"
                     /translation="IGREIAISALREWMAQIGSRATVAVSAVGKVKTALQMLALLLLL
                     YHEPLWGLPTRTLGIVALVIAAVLTLWSMLVYLAAAWRVLNK"
BASE COUNT          431 a         1141 c         1113 g          493 t
ORIGIN      
        1 gccgctgcag cgctcgcggc cggcgatatt gtggcgctgg cgctgccgtg gctgcggccc
       61 gaaggcaagg cgcggctgcg cagcgtgcgc gtcgtgcgcg tggcggcgcg cctcgggctg
      121 gcgctgctgc tgccgcgcct gctcgccgaa ggcgcggcgc tggccggcac ctccgactgg
      181 ttcgaggatg aaacctatgc agccctggcc cacaccgcct atccgctggc cgcggcgctg
      241 gcgctgcccg ggctgctggt gctcgcctgc ctgccgaaaa ccggcgaatg gaagcggggc
      301 tggctgctcg ccggtgccgt gccggcgctg ctgagcttcg gcgtcggcgg ccatgccgtg
      361 cacgcgctca ccggcggctg gctggcgccg gtgctggtcg tgcatgcggc ggctgcggcg
      421 atctggctcg gcgcgctgtg gccgaccgaa tatgcgctgc gcagcgttga cccgaagatc
      481 gacgaccagc ggccggcgct ggccgtgctc gggcgcttcg cgcatcgcgg gcgcgaactg
      541 ctcggcgcac tggccgtgag cggcctgctg atcgcctgcg tgcagctcgg tccgccgtcg
      601 ccggcctggg gcaacaccta cgcccggctg ttcatggcca agctcggact cgccctcctg
      661 ctgttcgcgc tcggcgcgct gaaccgctgg tgctggctgc cggcgctcgc ccgcggccgg
      721 cgccgggcgg cgagccggct gcggctgagc gtgcgcctct gccgcggcct gctggtggcg
      781 gtggtcgcgc tcagcgtcgc gctcggcacg acgatgccgc cgcgggccgg ggccggagcc
      841 gtggctccgc caccggcagc cctcgtcgag cggcactggc cgagcgccta cggaccccgg
      901 ctcgggctga acgtcgcgat gcaggccggc cggccggcgg cgacgctgag cgtcaccggc
      961 cccgacgatc agccgttcac gatccgcgag gccgtgctgg tgctcgaacg gcccgaagcc
     1021 ggcatcgagc cgctgcgccg gcggctggac gagcgggcgc cgggccggta cggcaacgac
     1081 gacctgctgc tgccgatcgc cggcccctgg cagggctacg tcgaaatctg gatcgacgac
     1141 ttctcgctcg aacgccagcc gctcgccttc acgctgccgt gagcatggca ctgcgcgttg
     1201 cccgcattcc ggccatgccg tattgcggct tgccggcgcc cggaccgagg tccgacgatg
     1261 cgcccgatcc cgcccgccgc gagccgccgc catgacgcag ccgaccgatg cccctgcctt
     1321 gccgacgctg gactggtact tcgattacct gtcgccgttc gcgtacttcc agctcgaacg
     1381 cttcacggcc gaaccgccgc cgggcgtggt cctgaggccg cagccggtgc tgctcggcgc
     1441 cttgctcggg gcctgcggcg cgcgcggccc ggccgagatt ccgctgatgc gcacgctgac
     1501 ctaccgccac tgcctgtggc aggcgcgtcg ccccggcctg ccgtaccgga tgccgccggc
     1561 ccatcccttc aatccgctgc cggcgctgcg gctgacggtc gcgctcggtg ccggccccga
     1621 caccgtgcgc acggtgttcc ggcatctgtg ggccgagggc cggacgctgg agggcgagga
     1681 ctggcaggcg ctgtgtgcgc ggctcgggct gtcggtggcc gaggccgggg cgctggccgc
     1741 gagtccggcc gcgaaggacg gcctgcgcga gcgcaccgaa gcggcgatcg cgcgcggcgt
     1801 gttcggcgtg ccgtgctcga tcgtccacgg cgaactgttc tggggcaacg acaccaccgg
     1861 catgctgctc gacttcctgg ccgatccggc gctgttccgt gatgcggaga tgcagcgcgt
     1921 cagcacgctg ccggccgcca tccagcgcag ggcctgagca tggcgcgcat cgcacacgcc
     1981 gaattcgagg aactctgccg cgagcacgtc cccttcgtcg gctcgctcgg catccgcgtc
     2041 gaaaccctgg acgccggccg ctgcgtgctg cgcgtgccgt ttcgcgacga cttcctgcgc
     2101 cccggcggca ccgtgtccgg tccgatccag atggcgcttg ccgacatcgt catgtacggc
     2161 gtcgtgctgt cgctgctcgg acgcgtcgat ctggcggtga ccaccaacct gacctgcaac
     2221 ttcctgcgcc gcccgaagcc ggtcgacctg atcggcgatg gccgcatcct gaagctcggc
     2281 aagcgcctgg cggtcggcga ggtgctgctg tattccgacg gcgagacgga accggtcgca
     2341 cacgtgacct gcacgtattc gattccgccg ggggccggac agcccatcgc gaattagccc
     2401 gcaagcgttg ttgcgtgctc ttcaggctga ccggcgtgtg cgtgaatgcc ggcgcgcgca
     2461 ggaatatctc cggcgcggcc ggcgggacga cgaaaggccg tacgccgggc atgccggaca
     2521 tcatgcatat aatgcagaaa ccctgcgctc cgggccgtcg atgacggtgg atcgcagggt
     2581 tccggcagga atcctgagtc gttaaaaact gcaggatcaa atagttggag gctagggtcg
     2641 gaatcgaacc ggcgtccacg gctttgcagg ccgctgcatg accactctgc cacctagcct
     2701 tcggcagtca ggtaacggca gtgctgccat taccggctga tccgaaaatc aaaaggcccc
     2761 gaaggaatcg aggcctctga tcgagaactg gagcgggaaa cgagactcga actcgcgacc
     2821 ctaaccttgg caaggttatg ctctaccaac tgagctattc ccgctcgacg tgggggcgca
     2881 ttataggccg cgaatccgga tcgtcaagcg ccggattcat ttattcagca cgcgccaggc
     2941 ggcggccaga tacaccagca tcgaccacag cgtcagcacc gccgcgatca ccagggcaac
     3001 gatgccgagc gtgcgcgtcg gcagccccca cagcggttcg tggtacagca gcagcagcag
     3061 tgccagcatc tgcagcgccg tcttgacctt gccgaccgcc gacaccgcca ccgtcgcgcg
     3121 gctgccgatc tgcgccatcc actcgcgcag cgccgagatc gcgatctcgc ggccgatg
//
LOCUS       DAOQJH010000313         3166 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_20180_length_3166_cov_3.663131, whole genome shotgun sequence.
ACCESSION   DAOQJH010000313 DAOQJH010000000
VERSION     DAOQJH010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3166
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20180_length_3166_cov_3.663131"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>3166
                     /locus_tag="PLQ18_07900"
     CDS             <1..>3166
                     /locus_tag="PLQ18_07900"
                     /inference="COORDINATES: protein motif:HMM:NF017930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HNE01611.1"
                     /translation="QESWFSLGRRLTAIGGEAALVSWSGSMFEYLMPPLVMPEYRGTL
                     LERTCAAAVARQIAYGRQRNVPWGISESGYNATDAQFNYQYRAFGVPGLGLKRGLGDD
                     LVIAPYATLMALPVDPPAAVRNLQRMAEAGFLGRHGFYEAIDYTPHRLPPNVTHAVVR
                     SFMAHHQGMAFLALAQTRLGPLMQQRFAADKALCATLLLLQERVPASAAYRSSEEAVG
                     SGPKPAAAGTLPERPNRSFDTADTALPQLVVLSNGSLNVLLTQAGGSRLERRGLALTR
                     WREDGTCDDRGLFFYLRDTASGAVWSAGFQPVRVRPPVFSATFSEGRAEFLRRDGDIE
                     LRMEVVVSPEDDIELRRLHLTNRGTSVRSIELTSFAEVALAPPAADAAHPAFSKLFVQ
                     TEAVPEAHALLATRRARGHGEAQPWLLHALLPRPEDPNTALAPASFETDRARFIGRTR
                     SSGAPLALAGAPGPLSGTTGAVLDPVIAIRHVFTIRPGESMIVDALTGGADSREAAQT
                     LLQKYRDPHMADRVFDLAWIHSQVVLRQLGVTETDAQLYWELAACVVFSDAHGRADAA
                     TLRQNRRGQSGLWSHAISGDLPIVLLRIQDVSNLDLARQLIQAHAYWRSKGLVADLVI
                     LNEDHASYRPLLLEQITGLIAAGIEASSTDKPGGIYVRNAEHVSHEDRVLLESVARFI
                     AWDRKGTLEQQLRRSATQPLLPPVLAPAADAVAPAAPVSLVREDLLLDNGFGGYTADG
                     REYLIRLRPDAPTPLPWANVIANAEGFGTVISECGSAYTWAVNAHEYRLTPWHNDPVS
                     DSCGEAFYVRDEDSGRYWSPTPLPARGRGDYLCRHGFGYSVFEHIEDGIVSELAVWVG
                     AGLPVKFSRVMLRNDSGRPRRLSLTGYVEWVLGEHRWKSAPHVITECDAICGALLAHN
                     AYSSEFPHMSAFFDADPGPQPAARSYTTDRTEFIGRNGSLAAPAAMGRTTLGNRTGAG
                     LDPCAAIRLPLTLAPGEQVELVFRLGAAPTHEVAQRAVAMRTPGVAWREWRAMQLHWE
                     ALLDTVQIDTPDPAVNLLGNGWLVY"
BASE COUNT          433 a         1181 c         1124 g          428 t
ORIGIN      
        1 caggagagct ggttctcgct cggacggcgg ctgaccgcga tcggcggcga ggcggcgctg
       61 gtgtcgtgga gcggctcgat gttcgagtac ctgatgccgc cgctggtgat gccggagtac
      121 cgcggcacgc tgctggagcg cacctgcgcg gccgccgtgg cgcgccagat cgcgtacggc
      181 cgccagcgca acgtgccctg gggcatctcg gaatccggct acaacgccac cgatgcgcag
      241 ttcaactacc agtaccgcgc cttcggcgtg ccgggcctgg gcctcaagcg cggcctgggc
      301 gacgatctgg tcatcgcgcc gtacgcgacg ctgatggcgc tgccggtcga tccgccggcg
      361 gccgtgcgca acctgcagcg gatggcggag gcgggcttcc tcggccggca cggcttctac
      421 gaggccatcg actacacgcc gcaccggctg ccgccgaacg tcacgcacgc ggtggtgcgc
      481 tcgttcatgg cccatcacca gggcatggcc ttcctggcgc tggcgcagac gcggctcgga
      541 ccgctgatgc agcagcgctt cgccgccgac aaggcgctct gcgcgacgct gctgctgctg
      601 caggagcgcg tgccggccag tgccgcctac cgcagcagcg aggaggccgt cggcagcggc
      661 ccgaagccgg ccgctgccgg caccctgccc gagcgcccga accgcagctt cgataccgcc
      721 gacacggcgc tgccgcagct cgtcgtgctg tccaacggca gcctgaacgt gctgctgacg
      781 caggccggcg gcagccggct ggagcgccgc gggctggcgc tgacgcgctg gcgcgaggac
      841 ggcacctgcg atgaccgcgg gctgttcttc tacctgcgcg acacggcttc gggcgccgtg
      901 tggtcggccg gtttccagcc ggtgcgcgtg cggcctccgg tgttctcggc gaccttctcg
      961 gaagggcgcg ccgagttcct gcgccgcgac ggcgacatcg agctgcgcat ggaggtcgtc
     1021 gtctcgcccg aggacgacat cgagctgcgc cgcctgcacc tgaccaaccg cggcacgagc
     1081 gtgcgcagca tcgagctgac cagcttcgcc gaggtggcgc tggcgccgcc ggccgccgat
     1141 gccgcgcatc cggccttcag caagctgttc gtgcagaccg aggccgtgcc cgaggcgcat
     1201 gcgctgctcg cgacgcgccg ggcgcgcggc cacggcgagg cgcagccctg gctgctgcat
     1261 gcgctgctgc cacggcccga ggacccgaac acggcgctgg cgccggcctc gttcgagacc
     1321 gaccgcgccc gcttcatcgg ccgcacccgc agcagcggcg ccccgctcgc gctcgccggc
     1381 gcgccgggac cgctgtccgg caccaccggc gccgtgctcg atccggtgat cgccatccgc
     1441 cacgtgttca cgatccgccc cggcgagtcg atgatcgtcg atgcgctgac cggcggggcc
     1501 gacagccgcg aggccgcgca gacgctgctg cagaagtacc gcgatccgca catggccgat
     1561 cgcgtgttcg acctcgcgtg gatccacagc caggtggtgc tgcgccagct cggcgtcacc
     1621 gagaccgacg cgcagctcta ctgggagctg gcggcctgcg tggtgttctc ggacgcccac
     1681 ggccgcgccg acgccgcgac cctgcgccag aaccggcgcg gacagtcggg gctctggagc
     1741 cacgcgatct cgggcgacct gccgatcgtg ctgctgcgca tccaggacgt gtcgaatctc
     1801 gatctggcgc ggcagctgat ccaggcgcac gcctactggc gcagcaaggg cctggttgcc
     1861 gatctggtga tcctgaacga ggaccacgcc agctaccggc cgctgctgct cgaacagatc
     1921 accggcctga tcgccgccgg catcgaggca agctcgaccg acaagcccgg cggcatctac
     1981 gtgcgcaacg ccgagcacgt ctcgcacgag gaccgcgtgc tgctggagtc ggtggcgcgc
     2041 ttcatcgcct gggaccgcaa gggcacgctg gagcagcagc tgcgccgcag cgccacgcag
     2101 ccgctgctgc cgccggtgct ggcgccggcc gccgatgccg tggcgccggc cgcgccggtc
     2161 agcctggtgc gcgaggacct gctgctcgac aacggcttcg gcggctacac ggccgacggc
     2221 cgcgagtacc tgatccgcct gcgccccgac gcgccgacgc cgctgccgtg ggcgaacgtc
     2281 atcgccaacg ccgaaggctt cggcaccgtg atctccgaat gcggctcggc ctacacctgg
     2341 gccgtcaacg cccacgagta ccgcctgacg ccgtggcaca acgatccggt cagcgacagc
     2401 tgcggcgagg ccttctacgt gcgcgacgag gacagcggcc gctactggtc gccgacgccg
     2461 ctgccggcgc gcggccgggg cgattacctc tgccgccacg gcttcggcta cagcgtgttc
     2521 gagcacatcg aggacggcat cgtcagcgag ctggcggtgt gggtcggggc cggcctgccg
     2581 gtgaagttct cgcgggtgat gctgcgcaac gacagcggcc ggccgcggcg gctgtcgctg
     2641 accggctacg tcgaatgggt gctcggcgag caccgctgga agtcggcacc gcacgtgatc
     2701 accgaatgcg acgccatctg cggcgcgctg ctcgcgcaca acgcctacag cagcgaattc
     2761 ccgcacatga gcgccttctt cgacgccgac cccggaccgc agccggccgc gcgcagctac
     2821 accaccgacc gcaccgagtt catcggccgc aacggctcgc tggccgcgcc ggcggcgatg
     2881 gggcgcacga cgctcggcaa ccgcaccggc gccggtctcg acccgtgcgc ggcgatccgg
     2941 ctgccgctga cgctcgcccc cggcgagcag gtggaactcg tgttccggct cggcgccgcc
     3001 ccgacccacg aggtcgcgca gcgggcggtg gcgatgcgca cgcccggcgt cgcgtggcgc
     3061 gaatggcggg cgatgcagct gcactgggag gcgctgctcg acaccgtgca gatcgacacg
     3121 cccgacccgg ccgtcaacct gctcggcaac ggctggctcg tctatc
//
LOCUS       DAOQJH010000314         3159 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_20262_length_3159_cov_3.748711, whole genome shotgun sequence.
ACCESSION   DAOQJH010000314 DAOQJH010000000
VERSION     DAOQJH010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3159
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20262_length_3159_cov_3.748711"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1549
                     /locus_tag="PLQ18_07905"
     CDS             <1..1549
                     /locus_tag="PLQ18_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019583678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HNE01612.1"
                     /translation="SPPPHHDIYSIEDLAQLIFDLKQVNPRALVSVKLVAEAGVGTIA
                     AGVAKAYADLITIAGYDGGTGASPLTSVKYAGSPWELGLTETHQTLRRNNLRDKVRLQ
                     TDGGLKTGLDVIKAAILGAESFGFGTGPMIAMGCKYLRICHLNNCATGVATQNNVLRL
                     NHYKGTVEKVMNYFRFIAQEVRELMAMLGITRFTDLIGRTDLLEILPGSTGKHARLDL
                     APILSDGGVAADKPRFCVEPHNAPFDKGELAEQMVRDTLAAIEAKRGGEFAYTVSNTV
                     RSIGARLSGEIARRYGDQGMNDAPVTLRLTGTAGQSFGVWNAGGLHMILEGDANDYVG
                     KGMAGGKLVIHPPRGSGFVEQDTTIIGNTCLYGATGGKLFAAALAGERFAVRNSGAIT
                     VVEGVGDHGCEYMTGGVVAVLGGTGVNFGAGMTGGFAFVLDEDNTFVDRYNHELIDIH
                     RISTEAMEPHRHYLRGLIEEFVAETGSPWGRQILADFRRYASKFWLVKPKAADLATLL
                     DTLLQAA"
     gene            1630..3045
                     /locus_tag="PLQ18_07910"
     CDS             1630..3045
                     /locus_tag="PLQ18_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006913024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNE01613.1"
                     /translation="MSKAKPMLFLDVSRQDPAKTPAEVRRHEFNEIYGQFDAKLAATQ
                     AGRCLHCGNPYCSWKCPVHNHIPHWLQLINEGNLFEAAELSHKTNSLPEICGRVCPQD
                     RLCEGACTLNDGFGAVTIGSVEKYITDEAIKAGWRPKLDHVKPTGKKVAIIGAGPAGL
                     GCADILVRNGVKAVVYDRHPQIGGLLTFGIPPFKLEKDVLQTRRELMEGMGVEFRLGV
                     EVGTDLSFQSLLDEYDAVFLGMGTYTYMKGGFPGEDLAGVHDALPFLISNVNRLLGYE
                     RSAADFIDVAGQRVVVLGGGDTAMDCNRTSIRQGATSVTCAYRRDEANMPGSKREVAN
                     AKEEGVKFLWNRQPVEIVGNGRVEGVKVVTTRLGAPDARGRRSPEVVPGSEEIIAADT
                     VLIAFGFRPDPKPWFAEFGIATDDRGRIAVRAAGSERQFQTGNPKVFAGGDMVRGSDL
                     VVTAVFEGREAALGILDYLEV"
BASE COUNT          524 a         1139 c         1042 g          454 t
ORIGIN      
        1 ctcgccgccg ccgcaccacg acatctactc gatcgaggac ctcgcgcagc tgatcttcga
       61 cctcaagcag gtcaacccgc gggcgctggt gtcggtgaag ctcgtcgccg aggccggtgt
      121 cggcacgatc gccgccggtg ttgccaaggc ctacgcggat ctcatcacga tcgccggcta
      181 cgacggcggc accggtgcct cgccgctgac ctccgtcaag tacgccggca gtccgtggga
      241 gctcggcctg accgagacgc accagacgct caggcgcaac aacctgcgcg acaaggtgcg
      301 gctgcagacc gacggcggcc tgaagaccgg cctcgatgtc atcaaggccg cgatcctcgg
      361 cgccgagagc ttcggcttcg gcaccggccc gatgatcgcg atgggctgca agtacctgcg
      421 catctgccac ctgaacaact gcgcgaccgg cgtggcgacg cagaacaacg tgctgcgcct
      481 gaaccactac aagggcacgg tcgagaaggt gatgaactac ttccggttca tcgcccagga
      541 agtccgcgag ctgatggcca tgctcggcat cacgcgcttc acggacctca tcggccgcac
      601 cgacctgctg gagatcctgc ccggcagcac cggcaagcac gcgcgcctcg atctggcacc
      661 gatcctctcc gacggcggcg tggcggccga caagccgcgc ttctgcgtcg agccgcacaa
      721 cgcgccgttc gacaagggcg agctggccga gcagatggtg cgcgacacgc tggcggcgat
      781 cgaggcgaag cgcggcggcg agttcgcata cacggtcagc aacaccgtgc gctcgatcgg
      841 tgcgcgcctc tccggcgaga tcgcgcgccg ctacggcgac cagggcatga acgacgcgcc
      901 ggtcacgctg cgcctgaccg gcaccgccgg ccagagcttc ggcgtctgga atgccggcgg
      961 cctgcacatg atcctcgaag gcgacgccaa cgattacgtc ggcaagggca tggccggcgg
     1021 caagctggtc atccacccgc cgcgtggctc gggcttcgtc gagcaggaca cgacgatcat
     1081 cggcaacacc tgcctgtacg gtgcgaccgg cggcaagctg ttcgcggcgg cgctcgccgg
     1141 cgagcgcttc gcggtgcgca actccggcgc gatcaccgtc gtcgagggcg tcggtgacca
     1201 cggctgcgag tacatgaccg gcggcgtggt cgcggtgctc ggcggcaccg gcgtcaactt
     1261 cggcgccggc atgaccggcg gcttcgcgtt cgtgctcgac gaggacaaca ccttcgtcga
     1321 tcgctacaac cacgagctga tcgacatcca ccgcatctcg accgaggcga tggaaccgca
     1381 ccgccactac ctgcgcggac tgatcgagga gttcgtcgcc gaaaccggca gcccgtgggg
     1441 ccggcagatc ctcgccgact tccgccgcta tgcgtcgaag ttctggctgg tcaagccgaa
     1501 ggccgccgat ctggccacgc tgctcgacac gctgctgcag gccgcctgac gcgctctgcc
     1561 gcgcaccggg gccccggccc cggtgccgcg gtgcctgccc gatatcgcct ctcacccaag
     1621 cggatcgtca tgtccaaagc caaaccgatg ctgttcctcg acgtgagccg tcaggatccg
     1681 gcgaagacgc cggccgaagt ccgccggcac gaattcaacg aaatctacgg ccagttcgac
     1741 gccaagctgg cggcgaccca ggccgggcgc tgcctgcact gcggcaaccc gtactgctcg
     1801 tggaagtgcc cggtgcacaa ccacatcccg cactggctgc agctgatcaa cgaaggcaac
     1861 ctgttcgagg cggccgagct gtcgcacaag accaactcgc tgcccgagat ctgcggccgc
     1921 gtctgcccgc aggatcgcct gtgcgaaggc gcctgcacgc tgaacgacgg cttcggcgcg
     1981 gtcacgatcg gttcggtcga gaagtacatc accgacgagg cgatcaaggc cggctggcgg
     2041 ccgaagctcg atcacgtcaa gcccaccggc aagaaggtcg cgatcatcgg ggccggcccg
     2101 gccggtctcg gctgcgccga catcctcgtg cgcaacggcg tcaaggccgt cgtctacgac
     2161 cgccatccgc agatcggcgg cctgctgacc ttcggcatcc cgccgttcaa gctcgaaaag
     2221 gacgtgctgc agacccggcg cgagctgatg gaaggcatgg gtgtcgagtt ccggctcggc
     2281 gtcgaggtcg gcaccgacct gagcttccag agcctgctcg acgagtacga cgccgtgttc
     2341 ctcggcatgg gcacgtacac gtacatgaag ggcggcttcc cgggcgagga cctcgccggc
     2401 gttcatgacg cgctgccctt cctgatctcg aacgtcaacc gcctgctcgg ctacgagcgc
     2461 agcgccgccg acttcatcga tgtcgccggc cagcgcgtcg tcgtgctcgg cggcggcgac
     2521 acggcgatgg actgcaaccg cacctcgatc cgccagggcg cgaccagcgt cacctgcgcg
     2581 taccgccgcg acgaggccaa catgcccggc tcgaagcgcg aggtcgccaa cgccaaggaa
     2641 gaaggcgtga agttcctctg gaaccgccag ccggtcgaga tcgtcggcaa cggccgggtc
     2701 gagggcgtca aggtggtcac cacccggctc ggcgcgcccg atgcccgcgg ccggcgcagc
     2761 cccgaggtcg tgcccggctc cgaggagatc atcgcggccg acaccgtgct gatcgccttc
     2821 ggcttccgtc cggacccgaa gccgtggttc gccgagttcg gcatcgccac cgatgatcgc
     2881 ggccgcatcg ccgtgcgcgc cgccggcagc gagcgccagt tccagaccgg caacccgaag
     2941 gtgttcgccg gcggcgacat ggtgcgcggt tccgatctgg tcgtgaccgc ggtcttcgaa
     3001 ggccgcgaag cggcactcgg catcctcgac tacctcgaag tctgagcctc ccgccccccg
     3061 cagcggctgc cgcaagcctc cggcggccgc tgcggcggcg catcgtggcc gatcggcgcg
     3121 cgatgcggca cactgccggc ctttccgcgc cgtgttgcc
//
LOCUS       DAOQJH010000315         3153 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_20332_length_3153_cov_5.369593, whole genome shotgun sequence.
ACCESSION   DAOQJH010000315 DAOQJH010000000
VERSION     DAOQJH010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3153
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20332_length_3153_cov_5.369593"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..836)
                     /locus_tag="PLQ18_07915"
     CDS             complement(<1..836)
                     /locus_tag="PLQ18_07915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE01614.1"
                     /translation="MNLAALLAETRLRLDDEAQPYRHADGVLTGWLNEAQREAAERAR
                     LFVDSATPEITRIALRDGVAAYPLDPRILFVQRARLDTAELVQVGIGDLLRSGCADWS
                     GEPRRYGLDMDAGTLTLLPAPDRATEDAVSVTAAVWLAGTLSVTTEAAIGASVGDLVT
                     LGGYADGAHVVTTVAGSAFGLAVADDPGAFVAATAARHRNLSLTVLRAPLADMEADDD
                     EPEIPARYHRDLIDWCCHLAYLLRDSDTESFQRATAFEQRFTQRFGPRLSARDHARLS
                     RNP"
     gene            complement(833..1000)
                     /locus_tag="PLQ18_07920"
     CDS             complement(833..1000)
                     /locus_tag="PLQ18_07920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE01615.1"
                     /translation="MSGDRYLRQINTGHIYAWAPELEDRPDMEPYEFPKPAPEKESEP
                     AKEPAKREAKP"
     gene            complement(997..1452)
                     /locus_tag="PLQ18_07925"
     CDS             complement(997..1452)
                     /locus_tag="PLQ18_07925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE01616.1"
                     /translation="MATVNKALGSGGALPANLRAVEVMARNFDFSTANGANGDIRHLI
                     LIPAGTLVKKVWLRVMTAEGATAIADVGDYLVSDGSVVDADGWLNDASLNAVALVSGS
                     TEAYAAAGGKLYTADSYLSLTLGTATTYDAAKGTLYAEVVNMTTPSDVA"
     gene            complement(1465..2388)
                     /locus_tag="PLQ18_07930"
     CDS             complement(1465..2388)
                     /locus_tag="PLQ18_07930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE01617.1"
                     /translation="MAYPAASGFNQNAGTLVPQTWAARLLANYYAATCLHSICNTNQV
                     NLIKDQGDTVIIRTTPTIAIKSGYKKGQKLEYDQPLTDRIELLIDKGDVYGFVVDDID
                     EKQSDVMFTDQWLIDATEQFKISVDRQAFSLVYPDAHAKNKGANSGKITGMYNMGAAG
                     APLAIDKNNAIDVIVNVNSVLTEQNVPKPGRWIVIPDWMRNKLMTSEIRQVQITGDSV
                     SPIRNGLIGRIDDTDIYVSNLLPYATDSGHYCNHILAGHRDAITFASQFVKNESFRAQ
                     DTFGTRYRGLQVWGIKVVKPEALVDVFAYAA"
     gene            complement(2506..>3153)
                     /locus_tag="PLQ18_07935"
     CDS             complement(2506..>3153)
                     /locus_tag="PLQ18_07935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE01618.1"
                     /translation="YDELRSMRSENDRLRAELAAMSRPEQTAPQQPANVVAGGEEYGF
                     GEGEAKWIADVARSALQDDIAALKQSAQAMEEQRAQAAAERFYADLDKAAPGWRETWQ
                     DAGFASWLDDNGVRQPLQAVVDANDARGIAFYINTYRQQKAATPAKPAAADRLAGMVE
                     PERGRAAELPAAKPMLRREDLHRLSMDFGGGRITKAEFDKQIVPFEAAEREGRLI"
BASE COUNT          476 a         1038 c         1018 g          621 t
ORIGIN      
        1 gggttgcgcg acagtcgcgc gtgatcgcgc gccgacagcc gcggcccgaa gcgctgcgtg
       61 aagcgctgct cgaacgcggt cgcgcgctgg aacgattcgg tgtcggagtc gcgcagcaga
      121 taggcgaggt ggcagcacca gtcgatcagg tcgcggtgat atcgggccgg aatctcgggc
      181 tcgtcgtcat cggcttccat gtcggccagt ggcgcgcgca gtacggtcag cgacaggttg
      241 cggtgccgcg ctgcggtggc ggcaacgaac gcgccggggt cgtcggcaac agcgaggccg
      301 aacgcactgc cggcaacggt cgtcacgacg tgcgcgccgt ccgcgtagcc gccgagcgtg
      361 accagatcgc cgaccgacgc accgatggcg gcctccgtcg tcaccgacag agtgccggcc
      421 agccagaccg ccgccgtgac gctgacggca tcctccgtag cgcgatcagg cgcaggcagc
      481 agcgtcagcg tgccggcatc catgtccagt ccgtagcggc gcggctcccc gctccagtct
      541 gcgcagccgg atcgcagcag atcgccgatg ccgacctgaa ccaactctgc cgtgtccagt
      601 cgtgcgcgct gcacgaacag gatgcgagga tcgagcgggt atgccgcaac gccatcacgc
      661 agcgcgatgc gtgtgatttc cggcgtggcg ctgtccacga acagccgcgc ccgctcggcc
      721 gcctcccgct gcgcctcgtt cagccaaccg gtcaacacgc catcggcgtg ccggtacggc
      781 tgcgcttcgt catcgaggcg caggcgggtt tcggcgagca gcgcggccag gttcacggct
      841 tggcctcacg cttcgccggt tccttcgctg gctctgattc cttttccggc gccggcttcg
      901 gaaactcgta tggctccatg tcggggcgat cttcgagttc cggcgcccat gcgtagatgt
      961 ggccagtgtt gatctggcgc aggtaccggt cgccgctcat gccacgtcac tcggcgtagt
     1021 catgttcacg acctcggcgt acagcgtgcc cttggccgcg tcgtaggtcg tggccgtgcc
     1081 gagcgtcagc gacaggtagc tgtcggccgt gtagagcttg cctccggccg ccgcgtaggc
     1141 ttcggtcgag ccggaaacca gcgccaccgc gttcagcgat gcatcgttga gccagccatc
     1201 cgcgtcgacc acgctgccgt cgctgacgag gtagtcgccc acgtccgcga ttgcggtcgc
     1261 cccttccgcg gtcatcacgc gcagccagac ctttttcacc agcgttccgg ccgggatcag
     1321 gatcaggtgg cggatgtcgc cgttcgcgcc gttggccgtc gagaagtcga agttgcgcgc
     1381 catgacctcc acggcgcgca ggtttgccgg cagagcgccg ccggagccga gtgccttgtt
     1441 tacggttgcc atgatcagcc ctcctcaggc cgcgtaggcg aatacatcga cgagggcttc
     1501 cggcttcacc accttgatgc cccatacctg caggccgcgg tagcgagtgc caaacgtgtc
     1561 ctgtgcccgg aacgactcgt tcttgacgaa ctgcgaggcg aacgtgatcg cgtcgcgatg
     1621 gccggcgagg atgtgattgc agtagtggcc ggaatcagtg gcatacggca gcaggttgct
     1681 gacgtagatg tcggtgtcgt cgatgcgccc gatcaggcca ttgcggatcg gcgacacgga
     1741 atcgccggtg atctgcactt gccggatttc ggacgtcatc agcttgttgc gcatccagtc
     1801 cgggatcacg atccagcggc ccggcttcgg cacgttctgc tcggtcagca ccgaattgac
     1861 gttgacgatc acgtcgatgg cgttgttctt atcgatggcg aggggagcgc cggcagcgcc
     1921 catgttgtac atgccggtga tctttccgga attcgcgccc ttgttcttgg cgtgcgcgtc
     1981 cgggtagacg agcgagaatg cctgccggtc cacggaaatc ttgaactgct cggttgcgtc
     2041 gatcagccac tgatccgtga acattacgtc ggattgcttt tcgtcgatgt cgtcaacgac
     2101 aaagccgtac acgtcacctt tgtcgatcag aagctcgatc ctgtcagtca gcggctgatc
     2161 gtattccagc ttctgaccct tcttgtaccc ggacttgatg gcgattgtcg gggtcgtgcg
     2221 gatgatgacc gtatcgccct gatccttgat cagattcacc tgattggtgt tgcagatgct
     2281 gtgcaggcaa gtggctgcgt agtagttggc gagcagtctt gctgcccacg tctgcggaac
     2341 aagcgtcccg gcgttctggt taaatccgga ggcggcagga tatgccatga ctgcaactcc
     2401 atgaaaaagg ggctgccgct ttcgcgcaca gcccctttgg gggtcggata gcagtcgtca
     2461 tcacgacgat tgcgttctca tgccgggctc gccccggctg gttgatcaga taagacggcc
     2521 ttcccgctcc gcggcctcga acggcacgat ttgcttgtca aactccgcct tggtgatccg
     2581 gcctccgccg aagtccatgc tgagcctgtg caagtcctcg cgcctcagca tcggctttgc
     2641 cgccggcaac tccgccgcgc gcccgcgctc cggctccacc atgccggcca gcctgtcggc
     2701 ggcagccggc tttgccggtg ttgctgcctt ctgctgccgg taggtgttga tgtagaacgc
     2761 gatgccgcgt gcatcgttgg cgtcaaccac ggcctgaagc ggttggcgca cgccgttgtc
     2821 gtcgagccac gaggcgaacc cggcgtcctg ccacgtctcc cgccagccgg gcgcagcctt
     2881 gtccagatcg gcatagaacc gttctgccgc ggcctgcgcc cgctgctctt ccatcgcctg
     2941 cgcggactgc ttgagcgcgg cgatgtcgtc ctgcagtgcg ctgcgcgcca cgtcggcgat
     3001 ccacttcgct tcgccttcgc caaacccgta ttcctcgcct cccgcaacga cgttggcggg
     3061 ctgctgcgga gcggtctgct cgggtctgct catggccgcc agttcggccc gcagccgatc
     3121 gttctcgctg cgcatggagc gcaactcgtc gta
//
LOCUS       DAOQJH010000316         3153 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_20333_length_3153_cov_5.124597, whole genome shotgun sequence.
ACCESSION   DAOQJH010000316 DAOQJH010000000
VERSION     DAOQJH010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3153
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20333_length_3153_cov_5.124597"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..277)
                     /locus_tag="PLQ18_07940"
     CDS             complement(<1..277)
                     /locus_tag="PLQ18_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013205688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HNE01619.1"
                     /translation="MTAPLVSRLPRVGTTIFTVMSALAQECGALNLSQGFPDFAVPPA
                     LLQAVVQHLQAGHNQYAPMTGVPRLREAIAAKTLAAYGVAPDPVHEVT"
     gene            884..2806
                     /locus_tag="PLQ18_07945"
     CDS             884..2806
                     /locus_tag="PLQ18_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrkA family serine protein kinase"
                     /protein_id="HNE01620.1"
                     /translation="MSIFERYATRYEESREEVLSLQEYLELCRSDPSAYASPAERLLM
                     AIGEPELIDTRLDPRLSRIFQNKVIKVYPAFREFYGMEDSIDQIVSYLRHSAQGLEER
                     KQILYLLGPVGGGKSSLAEKLKHLMEKVPFYAIQGSPVNESPLNLFDPDEDGRILEDD
                     FGIPRRYLKGLMSPWAVKRLKEFNGDITRFKVVRLHPSILKQVAVAKTEPGDENNQDI
                     SSLVGKVDIRKLEHYAQNDPDAYAFSGGLCLANQGLLEFVEMFKAPIKVLHPLLTATQ
                     EGNYNGTEGLSAIPFDGIILAHSNESEWMAFRNNRNNEAFLDRVYIVKVPYCLRVSDE
                     VHIYEKLLISSSLSEAPCAPGTLQMLAQFSVLSRLKEPENSSIFSKMRVYDGENLKDT
                     DPKAKSIQEYRDYAGVDEGMAGISTRFAFKILSQVFNFDHTEVAANPVHLLYVLERQI
                     EREQFPAEVEEKYLSYLKGWLAPRYAEFIGKELQQAYLESYSEYGQNIFDRYVTYADF
                     WIQDQEYRDPDTGESFDRASLNSELEKIEKPAGISNPKDFRNEIVNFVLRARAANNGR
                     NPVWTSYEKLRAVIEKKMFSNTEDLLPVISFNAKSSADEQKKHEDFVNRMTQKGYTAK
                     QVRLLSEWYLRVRKSS"
     gene            2841..>3153
                     /locus_tag="PLQ18_07950"
     CDS             2841..>3153
                     /locus_tag="PLQ18_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014087916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF444 family protein"
                     /protein_id="HNE01621.1"
                     /translation="MSHLVDRRLNGKNRSAVNRQRFLRRFKDQLKRAVADAMAGRSIK
                     DVDSGEKVSIPTRDLSEPVFSHGSGGRRETIHPGNKEFSTGDRVKRPQGGGGAGGGGK
                     QA"
BASE COUNT          645 a         1033 c          962 g          513 t
ORIGIN      
        1 cggtgacctc gtgcaccgga tcgggcgcaa ccccgtaggc cgcgagcgtc ttcgcggcaa
       61 tcgcctcgcg caggcgcggc acgccggtca tcggcgcgta ctggttgtga ccggcctgca
      121 ggtgctgcac gaccgcctgc agcagggccg gcggcacggc gaaatccgga aagccctgcg
      181 acaggttcag ggccccgcac tcctgtgcga gcgcactcat gaccgtgaag atcgtcgtgc
      241 cgacgcgcgg cagacgcgaa acgagcggcg ccgtcatgac gcggtctcct cgggatttca
      301 gctcggggtc ggagggaagc atgcggaccc gccagtgtac gggattcgcg cctgtacgag
      361 tccggcattg tcgacaggca tcgatgcacc aggaaggacg gcggctgcag cgccttgcgc
      421 cactgcaacg cacattcggg accgctcgcg caccattgcg tagcactctg caccatggca
      481 tgaaccagat tgatgcagta cgccattgca tggcggctcg attgcggcga tgcagcagaa
      541 cgcgccgggc ttgctacagt cgttcacgac tgccccccga cgccgggcgg gagcggcggc
      601 atacgctcgg cagcatgtcg tcgccggcag cgaccatgct gcggacggat gacaaaacgg
      661 ctacaagcgc tccgaaccgc cacccgccgt cacggtcctc cctgaggcag gcccgggacc
      721 ggaggcagca gatccggcgc caggcagcgc cagaacccca caccgtcgac ggcgacctgc
      781 accggtcggc ggtcccccct gcaggaatcc ccgaaattgg tccggctatt gctagtttcg
      841 ggacagatca tcgtgcattc cggcgccaga agagggttag gccatgagca tcttcgaacg
      901 atacgccacg cgatacgagg aaagccggga agaggtgctg agcctccagg aatacctgga
      961 actctgccgc agcgacccca gtgcctacgc ctcccctgcc gaacgcctgc tgatggcgat
     1021 cggcgagccg gaactgatcg atacgcgcct cgacccgcgc ctgtcgcgca tcttccagaa
     1081 caaggtcatc aaggtgtacc cggccttccg ggagttctac ggcatggagg attcgatcga
     1141 ccagatcgtc tcctatctgc gccactccgc gcagggtctg gaagaacgca agcagatcct
     1201 ctatctgctc ggcccggtcg gtggcggcaa atcctcgctg gccgagaaac tcaagcacct
     1261 gatggaaaag gtgccgttct atgccatcca gggttcgccg gtcaacgaat ccccgctgaa
     1321 cctgttcgat ccggatgagg acggccggat tctcgaggac gatttcggca ttccacgccg
     1381 ttacctgaaa ggcctgatga gcccgtgggc cgtcaagcgc ctgaaggaat tcaacggcga
     1441 catcacccgc ttcaaggtcg tgcgcctgca tccgtcgatc ctgaaacagg tcgcggtggc
     1501 caagaccgaa cccggtgacg agaacaacca ggacatttcc tcgctggtcg gcaaggtcga
     1561 catccgcaag ctcgaacatt acgcgcagaa cgatccggat gcgtatgcat tctcgggcgg
     1621 cctgtgcctc gcgaaccagg gcctgctgga attcgtcgag atgttcaagg caccgatcaa
     1681 ggtgctgcac ccgctgctga ccgccacgca ggaaggcaac tacaacggta cggaaggcct
     1741 gtcggcgatt ccgttcgacg gcatcatcct cgcacactcg aacgaatccg aatggatggc
     1801 gtttcgcaac aaccgcaaca acgaggcctt cctcgatcgc gtctacatcg tcaaggtccc
     1861 gtactgcctg cgcgtttccg atgaagtgca catctacgag aaactgctga tcagttcctc
     1921 gctgtccgaa gccccgtgcg cgcccggcac gctgcagatg ctggcgcagt tctcggtgct
     1981 gtcacgcctg aaggaaccgg aaaactccag catcttctcg aagatgcgcg tctatgacgg
     2041 cgaaaacctc aaggacaccg acccgaaggc caagtcgatc caggaatacc gcgactacgc
     2101 cggcgtcgac gagggcatgg ccggcatctc gacgcgcttc gcgttcaaga tcctgtcgca
     2161 ggtgttcaat ttcgaccaca ccgaggttgc cgccaatccg gtgcatctgc tgtacgtgct
     2221 ggagcgccag atcgaacgcg agcagttccc ggccgaggtc gaggagaaat acctgtccta
     2281 cctgaagggc tggctggcgc cgcgctatgc cgagttcatc ggcaaggaac tgcagcaggc
     2341 ctatctggaa tcgtattcgg aatacggcca gaacatcttc gaccgttacg tgacctacgc
     2401 ggacttctgg attcaggatc aggaataccg cgatccggat accggcgaat ccttcgaccg
     2461 cgcctcgctg aacagcgaac tcgaaaagat cgagaagccg gccggcatct ccaacccgaa
     2521 ggacttccgc aacgagatcg tcaacttcgt gctgcgtgcc cgtgccgcca acaacggccg
     2581 caacccggtc tggaccagtt atgaaaaact gcgcgccgtg atcgagaaga agatgttctc
     2641 gaacaccgag gacctgctgc cggtcatcag cttcaacgcc aagtcgtccg ccgacgagca
     2701 gaagaagcat gaggacttcg tcaaccgcat gacccagaag ggctacaccg ccaagcaggt
     2761 gcgtctgctt tccgagtggt acctgcgcgt gcgaaagtcg tcctgacgcc tgtcgcccct
     2821 gccccgcgag gaatgcttcc atgtcccatc tcgtcgatcg gcggctcaac ggcaagaatc
     2881 gcagcgccgt caaccggcag cgcttcctgc gccgcttcaa ggatcagctc aagcgcgccg
     2941 tggccgacgc catggcgggt cgctcgatca aggacgtgga cagcggcgag aaggtcagca
     3001 tcccgacccg cgacctgtcg gagccggtgt tcagccacgg ctccggcggt cgccgggaga
     3061 cgatccaccc cggcaacaag gagttctcga ccggcgaccg cgtcaagcgg ccgcagggcg
     3121 gtggcggggc cggcggcggc ggcaagcagg cca
//
LOCUS       DAOQJH010000317         3149 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_20379_length_3149_cov_4.807046, whole genome shotgun sequence.
ACCESSION   DAOQJH010000317 DAOQJH010000000
VERSION     DAOQJH010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3149
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20379_length_3149_cov_4.807046"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2432
                     /gene="gyrA"
                     /locus_tag="PLQ18_07955"
     CDS             <1..2432
                     /gene="gyrA"
                     /locus_tag="PLQ18_07955"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006786483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HNE01622.1"
                     /translation="GDVIGKYHPHGDTAVYDTIVRMAQPFSMRYMLVDGQGNFGSIDG
                     DAPAAMRYTEVRMSRIAHELLADIDKETVDFVPNYDGSETEPAVFPTRVPNLLVNGSA
                     GIAVGMATNIPPHNLGEVVDACIALIDDPTLGIPELMQYLPGPDFPTAAIINGSRGIR
                     DAYMTGRGRIYVRARTEIETDERTGRQTIVVTELPYQVNKARLLEKIAELVREKKLEG
                     ISELRDESDKDGMRMVIELKRGEVPEVVLNNLYQHTQMQSVFGINMVALLDGQPRLLN
                     LRQILEAFVRHRREVVTRRTIYELRKARERAHVLEGLSVALANIDAMIELIRRSPSPA
                     DAREGLMARVWEAGPVTAMLERAGAVSSRPEGLEAGLGLVGGGYRLSGAQAQAILDLR
                     LHRLTGLEQDRIHGEYQELLARIADLLDILASADRLMQVIRDELVELRTQYADARRTE
                     IQHTQVDLTLEDLISEEDMVVTLSHAGYAKAQPLTDYRAQRRGGRGKSATAMKDEDFI
                     DKLFIANTHDTLLCFSNRGKVYWIKVYELPQAGRVARGKPLVNLLPMEQGERINAVQP
                     VREFGEDQYLFFATTSGTVKKTPLSEYSRPRAAGIIAIDLRDGDELVDVAITDGRRDI
                     MLFTDAGKAIRFAETDVRPMGRNAAGVRGIRFKEEESDTDDEAAIDRSARVIALVVVG
                     ATGDILTVTENGFGKRTAPEEYPLRGRGGQGVISIQTSERNGAVVGAVQVDAADDVML
                     ITDGGTLVRTRVAEISVLSRNTQGVKLIAVQEGERLITVERIETDGEAAEDADSEPEA
                     GDGGDSDSGAG"
     gene            2484..>3149
                     /gene="serC"
                     /locus_tag="PLQ18_07960"
     CDS             2484..>3149
                     /gene="serC"
                     /locus_tag="PLQ18_07960"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019406881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HNE01623.1"
                     /translation="MSRVFNFSAGPAALPEPVLERARAELSDWHGHGMSVMEMSHRGR
                     DFTAIAEQAEADLRRLLGVPDDYAVLFVQGGASQQFAMVPLNLLPEGGSADYLDTGTW
                     SDKAIEEGARFGRINVVASSRDTRYTTIPAREGWRLDPQAAYVHYTPNETIVGVEFPE
                     VPEVGDRTLVADMSSTILSRPIDVRRFGLIYAGAQKNIGPAGLTVVIVRRELLGRARA
                     QCPT"
BASE COUNT          532 a         1083 c         1096 g          438 t
ORIGIN      
        1 tcggcgacgt gatcggcaag taccacccgc acggcgacac cgcggtctac gacaccatcg
       61 tgcgcatggc ccagccgttc tcgatgcgct acatgctggt cgacggccag ggcaacttcg
      121 gctcgatcga cggcgatgcg ccggcggcga tgcgttacac cgaagtgcgc atgagccgca
      181 tcgcgcacga gctgctggcc gacatcgaca aggaaaccgt cgacttcgtg ccgaactacg
      241 acggttccga gaccgagccg gcggtgttcc cgacccgcgt gccgaacctg ctggtcaacg
      301 gctcggccgg catcgcggtc ggcatggcga ccaacatccc gccgcacaac ctcggcgagg
      361 tcgtcgatgc ctgcatcgcg ctgatcgacg acccgacgct cggcatcccc gagctgatgc
      421 agtacctgcc gggcccggac ttcccgaccg ccgcgatcat caacggctcg cgcggcatcc
      481 gcgatgccta catgaccgga cgcgggcgca tctacgtgcg ggcgcgtacc gaaatcgaga
      541 ccgacgagcg caccggccgc cagaccatcg tcgtcaccga gctgccgtat caggtcaaca
      601 aggcgcggct gctcgaaaag atcgccgagc tggtgcgcga gaagaagctc gaaggcatct
      661 cggaactgcg cgacgagtcc gacaaggacg gcatgcgcat ggtcatcgag ctgaagcgcg
      721 gcgaagtgcc cgaggtcgtg ctgaacaacc tgtatcagca cacgcagatg cagagcgtgt
      781 tcggcatcaa catggtcgcg ctgctcgacg gccagccgcg gctgctgaac ctccggcaga
      841 tcctcgaagc cttcgtgcgt caccggcgcg aagtcgtcac gcggcgcacg atctacgagc
      901 tgcgcaaggc ccgcgaacgc gcccacgtgc tcgaaggcct gtcggtggcg ctcgccaaca
      961 tcgacgcgat gatcgagctg atccgccgct cgccgagtcc ggcggatgcc agggaagggc
     1021 tgatggcgcg cgtctgggag gccggaccgg tcacggcgat gctggagcgc gccggggcgg
     1081 tgtcctcgcg tcccgaagga ctcgaagccg ggctcgggct ggtcgggggc ggctaccggc
     1141 tctcgggcgc gcaggcgcag gcgatcctcg acctgcgcct gcaccggctg accgggctgg
     1201 agcaggaccg catccacggc gaataccagg agctgctcgc gcggatcgcc gacctgctcg
     1261 acatcctcgc gagtgctgac cggctgatgc aggtgatccg cgacgaactc gtcgagctgc
     1321 gcacgcagta cgccgacgcg cggcgcaccg agatccagca cacgcaggtc gacctgacgc
     1381 tggaggatct gatctccgag gaggacatgg tcgtcacgct gtcgcacgcc ggctacgcca
     1441 aggcgcagcc gctgaccgac taccgtgcgc agcgccgcgg cgggcgcggc aagtcggcga
     1501 cggcgatgaa ggacgaggac ttcatcgaca agctgttcat cgccaacacc cacgacacgc
     1561 tgctctgctt ctcgaaccgc ggcaaggtgt actggatcaa ggtctacgag ctgccgcagg
     1621 ccgggcgcgt cgcgcgcggc aagccgctcg tgaacctgct gccgatggag cagggcgagc
     1681 gcatcaacgc cgtgcagccg gtgcgcgagt tcggcgagga ccagtacctg ttcttcgcga
     1741 ccacctccgg cacggtcaag aagacgccgc tgtccgaata ctcgcgcccg cgcgccgccg
     1801 gcatcatcgc gatcgacctg cgcgacggcg acgagctggt ggacgtggcg atcaccgacg
     1861 gccggcgcga catcatgctg ttcaccgacg ccggcaaggc gatccgcttt gccgagaccg
     1921 acgtgcggcc gatgggccgc aacgccgccg gcgtgcgcgg catccgcttc aaggaagagg
     1981 aatccgacac cgacgacgag gccgcgatcg accgcagcgc gcgcgtgatc gcgctggtgg
     2041 tggtcggtgc caccggcgac atcctgaccg tcaccgagaa cggcttcggc aagcgcaccg
     2101 cgcccgagga atacccgctg cgcggccgcg gcggccaggg cgtgatctcg atccagacca
     2161 gcgagcgcaa cggcgcggtg gtcggtgcgg tgcaggtcga tgccgccgat gacgtgatgc
     2221 tgatcaccga cggcggcacg ctggtgcgca cgcgcgtcgc cgagatctcg gtgctgtcgc
     2281 gcaacaccca gggcgtgaag ctgatcgcgg tgcaggaagg cgagcggctg atcacggtcg
     2341 agcgcatcga gaccgacggc gaggccgccg aggatgccga cagcgagccc gaggccggtg
     2401 acggcggcga ctccgattcc ggtgccggct gaggccggcg gcacgggcag gtccgatttt
     2461 tcctctgttc gaggcgagtt ccgatgtcga gagtgttcaa tttcagcgcc ggtcccgcgg
     2521 cgctgcccga gccggtgctc gaacgtgcgc gcgcggagct cagcgactgg cacggccacg
     2581 gcatgtcggt catggagatg tcgcaccgcg gccgtgactt caccgccatc gccgaacagg
     2641 ccgaggccga tctgcgccgg ctgctcggcg tgccggacga ctacgcggtg ctgttcgtgc
     2701 agggcggggc ctcgcagcag ttcgcgatgg tgccgctgaa cctgctgccc gagggcggct
     2761 cggccgatta cctcgatacc ggcacctggt cggacaaggc gatcgaggag ggcgcgcgct
     2821 tcgggcgcat caacgtcgtc gccagcagcc gcgacacgcg ctacacgacg atcccggcgc
     2881 gcgagggctg gcggctcgat ccgcaggccg cctatgtgca ttacacgccg aacgagacca
     2941 tcgtcggcgt cgagttcccg gaggtgcccg aggtcggcga ccggacgctc gttgccgaca
     3001 tgtcctcgac gatcctgtcg cgccccatcg acgtgcggcg cttcgggctg atctacgccg
     3061 gtgcgcagaa gaacatcggc ccggccgggc tgaccgtcgt catcgtgcgc cgcgaactgc
     3121 tcggccgcgc ccgcgcgcag tgcccgacg
//
LOCUS       DAOQJH010000318         3137 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_20513_length_3137_cov_3.728423, whole genome shotgun sequence.
ACCESSION   DAOQJH010000318 DAOQJH010000000
VERSION     DAOQJH010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3137)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3137)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3137
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20513_length_3137_cov_3.728423"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2787)
                     /locus_tag="PLQ18_07965"
     CDS             complement(<1..2787)
                     /locus_tag="PLQ18_07965"
                     /inference="COORDINATES: protein motif:HMM:NF017081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNE01624.1"
                     /translation="MLHASSLLPPLTPALRGRLALLAAGYGVFAVVAILLSRQPGSLA
                     TVWFANAWALVFWLRSRQVLPRRLQALAVLVAAWIANYVCNTPATLSLAFAGVNLAEI
                     VFGGLLIERHARERPESAGAMLRLLVFGVFVPPLLGATLGALLLQIAGIAEAVPVWPL
                     WYCGSVIGALALLPLGVTATRAWGDARGRLETLAGVLGAALVAGLVLPNLAFPYVYVT
                     LPLVLAAMRLPVAGTALAVCVTTAVWALLLAFERIPASEGGPVWTLLTPMIVTLLPAL
                     LLAAAVAETRMQTRRLANRERFVRLVTDALPGMVGYWDSGLRCRYANRAYREWFGREP
                     ESLYGETMQSLLGPALFAKNEPYVRAVLRGEPQRFERTLVKADGSTGHTWAHYIPDCP
                     GPDRPGSGRPDGVNGPVAGFFVLVTDVTELKAAELALARLNAELAERSVQAEAANRAK
                     SEFIANVSHEIRTPMNGILGLLQVLEDTALDSRQRRYLRTIRQSARALLDILNNILDH
                     SRIEAGRLDLHREDFDLPELLEGVVDLFAPAAHQKRLELLLDAPAGLPARLTGDALRL
                     HQVLVNLLGNALKFTERGEICLGVERLPGAAPGCARLRLQVRDTGIGMNEAQLQRLFT
                     PYVQADGSTARRYGGTGLGLVISQQLVRLMGGEITVSSAPGAGSTFGFELELPLAGPP
                     VRSLAPVPGRLAGVRVLLADAGATARGIVGRALEHWGCDCVEAHDADALLDTALSAAG
                     SGRPFRFCLIDFRLPPQGALKAQQRLLAERNAGRLPASTTILLLTEGERLELDGEPLA
                     AGLPVLMKPVTASRLHDVLIEHLDHELGIDNTSVPAPGEDWHRLGAVLRGAQVLVVED
                     NAVNRMVAEELLERLGIRVTAAADGGSGVELALAGRYDAVLMDLHMPGMDGFEATARI
                     RADGRHDALPIIAMT"
BASE COUNT          485 a         1140 c         1129 g          383 t
ORIGIN      
        1 ggtcatcgcg atgatcggca gcgcatcgtg ccggccatcg gcgcggatgc gcgcggtggc
       61 ctcgaagccg tccatgcccg gcatgtgcag gtccatcagc accgcgtcat agcgcccggc
      121 cagcgccagc tcgacgccgc tgccgccgtc ggcggcagcg gtgacgcgga tgccgagccg
      181 ttcgagcagt tcctcggcga ccatgcggtt gacggcgttg tcctcgacca cgagcacctg
      241 cgcgccgcgc agcaccgcac cgagccggtg ccagtcctcg cccggagccg gcacgctggt
      301 gttgtcgatc ccgagttcat ggtccaggtg ttcgatcagc acgtcgtgca ggcgcgaggc
      361 ggtgaccggc ttcatcagca ccggcagccc ggcggcgagc ggctcgccgt cgagttcgag
      421 ccgctcgccc tcggtcagca gcaggatcgt cgtcgaggcc ggcagccggc cggcgttgcg
      481 ctcggccagc agccgctgct gcgccttcag cgcgccctgc ggcggcagcc ggaaatcgat
      541 caggcagaag cggaacggcc ggccggaccc ggcggccgag agcgcggtgt cgagcagcgc
      601 atccgcgtca tgggcctcga cgcagtcgca gccccagtgc tccagcgccc ggccgacgat
      661 gccgcgggcg gtggcgccgg catcggcgag cagcacgcgc acgccggcga gccgcccggg
      721 caccggtgcg aggctgcgca ccggcgggcc ggcgagcggc agttcgagtt cgaaaccgaa
      781 ggtcgaaccg gcacccggcg cgctgctcac cgtgatctcg ccgcccatca ggcgcaccag
      841 ctgctggctg atgacgaggc cgagaccggt gccgccgtag cggcgcgcgg tcgagccgtc
      901 ggcctgcacg tagggcgtga acagccgctg cagctgggcc tcgttcatgc cgatgccggt
      961 gtcgcgcacc tgcaggcgca gccgcgcgca gccgggagcg gcaccgggca ggcgctcgac
     1021 gccgaggcat atctcgccgc gctcggtgaa cttcagcgcg ttgccgagca ggttcaccag
     1081 cacctgatgc aggcgcagcg catcgcccgt gaggcgcgcc ggcagcccgg ccggggcatc
     1141 gagcagcagc tccagccgct tctggtgcgc agccggtgcg aacaggtcga cgacgccttc
     1201 gagcagttcg ggcaggtcga agtcctcgcg atgcaggtcg aggcggccgg cctcgatgcg
     1261 cgagtgatcg aggatgttgt tcaggatgtc gagcagcgcg cgcgccgact ggcggatcgt
     1321 gcgcaggtag cggcgctggc ggctgtcgag cgcggtgtcc tccagcacct gcagcaggcc
     1381 gaggatgccg ttcatcggcg tgcggatctc gtggctgacg ttggcgatga actcgctctt
     1441 ggcgcggttg gcggcctcgg cctgcacgct gcgctcggcc agttcggcgt tgagcctggc
     1501 gagcgcaagc tcggccgcct tcagctcggt gacatcggtg accagcacga agaagccggc
     1561 gaccggcccg ttcacaccgt ccggacggcc ggagcccgga cggtcagggc ccggacagtc
     1621 aggaatgtag tgcgcccagg tgtggccggt gctgccatcg gccttgacca gcgtgcgctc
     1681 gaagcgctgc ggctcgccgc gcagcaccgc acgcacgtag ggttcgttct tcgcgaacag
     1741 cgccggcccg agcagcgact gcatcgtctc gccgtacagg gattccggct cgcggccgaa
     1801 ccattcgcgg taggcacggt tcgcgtagcg gcagcgcaga cccgagtccc agtagccgac
     1861 catgcccggc agcgcatcgg tgacgaggcg cacgaagcgt tcgcgattgg ccaggcgccg
     1921 ggtctgcatc cgggtctcgg cgaccgcggc ggccagcagc agcgccggca gcagcgtcac
     1981 gatcatcggc gtcagcagcg tccagaccgg ccccccctcc gacgccggaa tgcgctcgaa
     2041 ggcgagcagc agcgcccaga ccgccgtggt gacgcagaca gccagcgccg tgccggccac
     2101 cggcaggcgc atggccgcga gcaccagcgg cagcgtcacg tagacatacg gaaacgccag
     2161 attcggcagc accagcccgg cgaccagcgc cgcccccagg actccggcca gggtttcgag
     2221 ccgcccgcgg gcatcgcccc aggcccgcgt cgccgtcacg ccgagcggca gcagcgccag
     2281 cgcgccgatc accgacccgc agtaccacag cggccacacc ggcacggctt cggcgatgcc
     2341 ggcgatctgc agcagcagcg ccccgagcgt cgcgccgagc agcggcggca cgaacacgcc
     2401 gaagacgagc aggcgcagca tcgcgccggc gctctcgggc cgctcgcggg catggcgctc
     2461 gatcagcagg ccgccgaaca cgatctcggc gagattgacg ccggcgaagg caagcgacag
     2521 cgtcgccggc gtgttgcaga cgtagttggc gatccaggcc gccaccagca cggcgagtgc
     2581 ctgcagccgc cgcggcagca cctgacggct gcgcagccag aacaccagcg cccaggcatt
     2641 cgcgaaccag acggtcgcga ggctgccggg ctggcgcgac agcaggatgg cgacgacggc
     2701 gaacacgccg tagccggccg ccagcaaggc cagtcgcccc cgcagggccg gcgtcagcgg
     2761 cggcagcaat gacgaagcgt gcagcatgat cccgcgcacc atccggtcga gggcggcccg
     2821 gcctcccggc tggtgccgcg gtcatgaagg gggtcccggc gcaggccgcc cggccactcc
     2881 tgagcaaaac atcgcatccc tgacgcgccg cggaccgcgc actatgcctg ttgcccggcg
     2941 caggcggcag cccccggagg cagcgatacg aacatgggcg agcgaagcgg cctgctgcgc
     3001 gccgatctgc gcatcgacga cctcgaccgc gggcatcacg gtgtctatgc cgtgcgcctc
     3061 gcgcgccatc cgtccgaaac cgccgaacgg ctgatgatcc gcctgctggt ctttgcgctg
     3121 aatgtgcccg acgacgg
//
LOCUS       DAOQJH010000319         3132 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_20567_length_3132_cov_4.237894, whole genome shotgun sequence.
ACCESSION   DAOQJH010000319 DAOQJH010000000
VERSION     DAOQJH010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3132
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20567_length_3132_cov_4.237894"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..536
                     /locus_tag="PLQ18_07970"
     CDS             <1..536
                     /locus_tag="PLQ18_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018953679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HNE01625.1"
                     /translation="RGSLSVDDEGTPGECTVLIEHGVLRNYMQDKQNARLMGMRPTGN
                     ARRESYAHLTMPRMTNTYMLPGAHDPAEIVASVERGLYAVNFGGGQVDITSGKFVFSA
                     SEAYLIENGRVTRPVKGATLIGNGPEAMKRVSMVGNDLKLDLGVGTCGKDGQSVPVGV
                     GQPTLRLDGITVGGTKT"
     gene            1066..2673
                     /locus_tag="PLQ18_07975"
     CDS             1066..2673
                     /locus_tag="PLQ18_07975"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF012630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031012 - extracellular matrix [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HNE01626.1"
                     /translation="MPAPASGPVRRRPAAAGGFRSLPLAGALLCGLGLAVPAQAFSIL
                     GVNAGGNLTSALGVDEMFRWNGIATVGGVRTIGYRLDPGFTLAQQAAIDRAFATWNTV
                     ASAQPNMIASPGAFDLETVAVHEIGHALGLAHSGVREGDTPNPRFFDTDQNAFNLMPN
                     PDRTTLARNVTSRPTVDFFPGPGLSSSVMSYDASLAPGYLRTQLEHDDVGGLRYAGAG
                     PDNLSGNGDDFEYRFVRINAAPIQDFNAFGQLVPGIDIVRNPTEHIDAGIIDVFNGNP
                     FGAGGIADMVYDRTGFTGNGTFSILGGDIYLSATTLEDPYRSFPGVFTFTDPACDADD
                     VCNGLGTAHFDTGQNGRTPFNVNFDYDSAVYPVQPAGVFRLGTLAVTNGETFAGSVPE
                     IGNTSNFHLNLHLASSDGANVGDLLLGYYSSPNVFPNLRDPGNADILYDARHPEFGGV
                     YILEGNTLAEALTNVNLLGHLGSIEFDGFELPPGPFADGNSFLAPLPGGSFTGPQGSF
                     LIGVPEPASLLLLSAALPLLHRRRCPA"
     gene            complement(2756..>3132)
                     /gene="yjgA"
                     /locus_tag="PLQ18_07980"
     CDS             complement(2756..>3132)
                     /gene="yjgA"
                     /locus_tag="PLQ18_07980"
                     /inference="COORDINATES: protein motif:HMM:NF003593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HNE01627.1"
                     /translation="PEDLAGAIAECRRISAHGGRYRQMQYIGKLMRRIDPQPILDVLA
                     RFDSTSAESKRRQHQVERWVEAMVAGDETVLGEYLTAYPQADRQQIRQLIRNAQRELS
                     TGKPPKSRRELFRLLRAGAWQD"
BASE COUNT          470 a         1129 c         1052 g          481 t
ORIGIN      
        1 ggcgcggctc gctcagcgtc gatgacgagg gcacgcccgg cgaatgcacg gtgctgattg
       61 agcacggcgt gctgcgcaac tacatgcagg acaagcagaa cgcccggctg atgggcatgc
      121 gcccgaccgg caacgcccgg cgcgagtcct acgcgcacct gaccatgccg cgcatgacca
      181 acacctacat gctgcccggc gcgcacgatc cggccgagat cgtcgcctcg gtcgagcgcg
      241 gcctgtacgc ggtcaacttc ggcggcggcc aggtcgacat cacctcgggc aagttcgtgt
      301 tctcggcgag cgaggcctat ctgatcgaga acggcagggt cacgcggccg gtcaagggcg
      361 cgacgctgat cggcaacggc cccgaggcga tgaagcgcgt cagcatggtc ggcaacgacc
      421 tgaagctcga cctcggcgtc ggcacctgcg gcaaggacgg ccagagcgtg ccggtcggcg
      481 tcggccagcc gacgctgcgg ctcgacggca tcacggtcgg cggcaccaag acctgagccg
      541 gagcgcggct gccggctccg tccttcgtcc gaagcgaccg gggccggtcc ggcgctgccg
      601 gcgatggcgc aggatgcggc aaggggtgcg tcgcgccgca tccggcggct gaagtccggt
      661 cccgggcatc cgccggggtg tcgcacgggc actcatttgt gtcagacaga tttacagggc
      721 agcccggtac agtcggggtg tctctgcgca aacgtatgat tgaaaggcaa atgccggacg
      781 tggcctgcct gatgcaatct ccgttgcaac cggcgtctcg ccggtgcggc ggacatgcga
      841 cccgtgtcca ccgggcacgg gccctgcgtc cggggtccgt acggcggcgc accggcgtcg
      901 cccggcggtc agggccaggc aggcaaagac acagggcgga ggcacccgga ccgagcgggg
      961 gaggagaccg gttcacgccg cggtcgctca ccgacggggg ccgtcatgaa gcgcaccgca
     1021 ctgcttcttt cgtctgatcc cttccctgtt cctgccgcga atcatgtgcc ggctcccgcg
     1081 tccggccccg tccgccggcg accggctgcc gccggcggtt tccgcagcct cccgctcgcc
     1141 ggcgcgctgc tctgcgggct cggcctcgcg gtgccggcgc aggccttctc gatcctcggc
     1201 gtcaacgccg gcggcaacct caccagcgcg ctcggcgtcg acgagatgtt ccgctggaac
     1261 ggcatcgcga cggtcggtgg cgtcaggacc atcggctacc ggctcgatcc gggtttcacg
     1321 ctggcgcagc aggcggcgat cgaccgggcg ttcgcgacct ggaacacggt cgcgagcgcg
     1381 cagccgaaca tgatcgcgtc gccgggggcc ttcgatctcg aaaccgtggc ggtgcacgag
     1441 atcggccatg cgctcgggct cgcgcactcc ggcgtgcgcg agggcgacac gccgaatccg
     1501 cgcttcttcg acaccgacca gaacgccttc aacctgatgc cgaatccgga tcgcacgacg
     1561 ctggcgcgca acgtcacgag ccgcccgacc gtggatttct tccccggtcc cggcctgtcc
     1621 tcgagcgtga tgagctacga cgccagcctc gcgccgggct atctgcgcac gcagctcgaa
     1681 cacgacgatg tcggcggcct gcgctatgcc ggtgccggcc ccgacaacct ctccggcaac
     1741 ggcgacgact tcgaataccg cttcgtgcgc atcaatgctg cgccgatcca ggatttcaac
     1801 gccttcggcc agctcgtgcc cggcatcgac atcgtccgca acccgaccga acacatcgat
     1861 gccggcatca tcgacgtgtt caacggcaac cccttcggtg ccggcggcat cgccgacatg
     1921 gtctacgacc gcaccggctt caccggcaac ggcacgttct cgatcctcgg cggcgacatc
     1981 tacctgagcg cgacgacgct ggaggacccg taccggagct tcccgggtgt gttcacgttc
     2041 accgatccgg cctgcgacgc cgacgacgtc tgcaacggtc tcggcaccgc gcatttcgat
     2101 accggccaga acggccgcac accgttcaac gtcaacttcg actacgacag cgccgtctat
     2161 ccggtgcagc cggccggcgt gttccggctc ggcacgctgg ccgtgaccaa cggcgaaacc
     2221 ttcgcaggct cggtgccgga gatcggcaac accagcaact tccacctgaa cctgcatctg
     2281 gcgtcgagcg acggcgccaa cgtcggcgac ctgctgctcg gctattactc gtcgccgaac
     2341 gtgttcccga acctgcgcga tccgggcaac gccgacatcc tctacgacgc gcgccatccg
     2401 gagttcggcg gcgtgtacat cctggaaggc aacacgcttg ccgaggcgct gaccaacgtc
     2461 aacctgctcg ggcatctcgg ctcgatcgag ttcgacggct tcgaactgcc gcccgggccg
     2521 ttcgccgacg gcaacagctt cctcgcgccg ctgccgggcg gttcgttcac gggaccgcag
     2581 ggcagcttcc tgatcggcgt gccggagccg gcgtcgctgc tgctgctgtc ggcggcgctg
     2641 ccgctgctgc atcggcgccg ctgcccggcc tgaagcctgc ccctgtccgc acgaccggcc
     2701 cccggcccct gcctgccggg ggccgccgcg cgggctgacc ggccggcttg ccggatcagt
     2761 cctgccaggc gccggcgcgc agcagccgga acagctcgcg gcgcgacttc ggcggcttgc
     2821 cggtcgagag ttcgcgctgg gcattgcgga tcagctggcg gatctgctgg cgatcggcct
     2881 gcgggtaagc ggtcaggtac tccccgagca cggtctcgtc gccggcgacc atcgcctcga
     2941 cccagcgctc gacctgatgc tggcggcgct tcgactcggc gctggtcgag tcgaagcgcg
     3001 cgagcacgtc gaggatcggc tgcggatcga tgcgtcgcat cagcttgccg atgtactgca
     3061 tctgacggta gcgtccgccg tgtgcgctga tgcgccggca ttcggcgatc gcgccggcga
     3121 gatcctcggg ca
//
LOCUS       DAOQJH010000320         3121 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_20695_length_3121_cov_4.068167, whole genome shotgun sequence.
ACCESSION   DAOQJH010000320 DAOQJH010000000
VERSION     DAOQJH010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3121
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20695_length_3121_cov_4.068167"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(251..1105)
                     /locus_tag="PLQ18_07985"
     CDS             complement(251..1105)
                     /locus_tag="PLQ18_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017841686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HNE01628.1"
                     /translation="MNDCKLIPAPAPLTAKDYRSDLKPVWCAGCGDYAVIAAITRALA
                     ELQLPREDVAFVSGIGCSSRIPAYSAVYGFHGVHGRALPLATGLKVARPDLTVLVAGG
                     DGDGFSIGGNHFLHACRRNADVTYIVMDNSVYGMTKGQASPTTAADWTGSVLTPDGPG
                     VPAFRPLEIALAAGASFIARAFSGDPTTLAAQIVAGIRHEGFSLIHVLCPCVTYRPEQ
                     QKEFKARVHAEWQPTDDRREAFGRLLDDDGYATGILYRNPRPAYRAPQVAATTLDAIA
                     AGFEVAAA"
     gene            complement(1111..2880)
                     /locus_tag="PLQ18_07990"
     CDS             complement(1111..2880)
                     /locus_tag="PLQ18_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018717902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HNE01629.1"
                     /translation="MDPSLNPQSVSIVLCGSGGAGVMTAGQMLLDAAAAAGLYGVMSR
                     ALGPQIRGGEAAALLRLANHPVEAPDDAFDVLLAVDWQNIERFAAELPLRADSLVIGD
                     PAAGELPAAILRAHPRRAEVALQALADETGSRVNMVALGLLAELIGLPAEAVYATLAA
                     ALADKGEAVVESGIRAALAGAQVVAALPAVPRLPAVAAAANRPVRWSLSGNEASGLGA
                     LRGGVRFVAAYPITPATEILEWLAPRLPPLGGCLVQAEDELASVNMILGGAFGGVPSL
                     TATSGPGLALMTESLGLGVASETPVVVCNVMRGGPSTGIPTKSEQSDLDIALHGLHGD
                     APHLVTAATGIADCAFTTQWTVQLAEALQVPAIVLSDQAMGQSRAVVDALPDPGFRAV
                     RLTAPAGVEGYQRYAVTESGVSPMAVPGTPGGSFVADGLEHAPRGTPSTQAAHHREQL
                     DKRHRKLEVHDYGEHWALLEGARDAETAVICFGSLTAAAREAVDRLNAAGTQVRLISL
                     RLLAPALPLAFAAALRGVRHALVVEQNHSRQFLGHLRSHYPLQVHTHGYHRPGPVAIR
                     PGEIVAAVRSLLAPVEAPVTVED"
BASE COUNT          446 a         1047 c         1211 g          417 t
ORIGIN      
        1 aacaaggaga gcgccaagat cgagtgcggc aaggcccact tcaaggcgct ggccgtcggc
       61 gagaacccgg cgcagtacat ggtggcccga aaccttgagg atgtcctgct ccgcgcgggg
      121 ctctgacaag gaggggcagt ccaccagttt tctgtcgtga tccggtttcc gcgggcagtt
      181 cggaatccgg ccgccttgtg atggctctgc caccggggca gagcctcgcg catcgattcc
      241 cggtccgccc tcaggccgcc gccacctcga agccggcggc gatcgcgtcc agcgtcgtcg
      301 ctgccacctg cggcgcgcgg taggccgggc gcgggttgcg gtacaggatg ccggtcgcgt
      361 agccgtcgtc atcgagcagg cggccgaagg cttcgcggcg gtcgtcggtc ggctgccatt
      421 cggcgtgcac gcgcgccttg aactccttct gctgctcggg gcggtaggtc acgcacgggc
      481 agagcacgtg gatcagcgag aagccttcgt ggcggatgcc ggcgacgatc tgcgcggcca
      541 gcgtggtcgg atcgccggag aaggcgcggg cgatgaagct cgcgccggcg gcgagtgcga
      601 tctccagcgg ccggaacgcc ggcacgccgg ggccgtcggg cgtcagcacg ctgccggtcc
      661 agtcggcggc cgtggtcggc gaggcctggc ccttggtcat gccgtagacg ctgttgtcca
      721 tgacgatgta ggtcacatcg gcgttgcgcc ggcaggcgtg caggaagtga ttgccgccga
      781 tcgagaagcc gtcgccgtcg ccgccggcga ccagcacggt cagatcgggc cgcgcgacct
      841 tgaggccggt ggcgagcggc agcgcccggc cgtgcacgcc gtggaagccg tagacggccg
      901 agtacgccgg gatgcgcgac gagcagccga tgccggagac gaaggccacg tcctcgcgcg
      961 gcagctgcag ttcggcgagc gcgcgcgtga tcgcggcgat gacggcgtag tcgccgcagc
     1021 cggcgcacca gaccggcttg aggtcggagc ggtagtcctt ggcagtcagc ggggctggcg
     1081 cggggatgag cttgcagtcg ttcatggcgg tcagtcctcg acggtcacgg gcgcctcgac
     1141 cggcgcgagc agggagcgga cggcggcgac gatctcgccg gggcggatcg cgaccgggcc
     1201 gggccggtgg tagccgtgcg tgtgcacctg cagcgggtag tggctgcgca gatggccgag
     1261 gaactgccgg ctgtggttct gttcgacgac gagcgcatgg cgcacgccgc gcagcgccgc
     1321 ggcgaaggcg agcggcagcg ccggggcgag caggcgcagc gagatcagcc gcacctgcgt
     1381 gccggcggcg ttgagccggt cgaccgcctc gcgtgccgcg gcggtcagcg agccgaagca
     1441 gatcacggcg gtctcggcgt cgcgcgcgcc ttcgagcagc gcccagtgct cgccgtagtc
     1501 atggacttcg agcttgcggt ggcgcttgtc gagctgttcg cggtggtgcg cggcctgcgt
     1561 cgacggtgtg ccgcgcggcg cgtgttcgag gccgtcggcg acgaagctgc cgcccggcgt
     1621 gccgggcacg gccatcggcg agacgccgga ttcagtgacg gcgtagcgct ggtagccctc
     1681 gacgccggcc ggcgcggtca ggcgcacggc gcggaaaccc ggatcgggca gcgcatcgac
     1741 gacggcgcgc gactggccca tggcctgatc gctcagcacg atcgccggca cctgcagcgc
     1801 ctcggcgagc tgcacggtcc actgcgtcgt gaacgcgcag tcggcgatgc cggtggcggc
     1861 ggtgacgagg tgcggtgcat cgccgtgcag gccgtgcagc gcgatgtcga ggtcggactg
     1921 ctcggacttg gtcgggatgc cggtcgacgg gccgccgcgc atgacgttgc agaccacgac
     1981 cggggtttcg gaggccacgc cgaggccgag cgattcggtc atcagcgcca ggcccggccc
     2041 ggaggttgcg gtcagcgacg gcacgccgcc gaaggcgccg ccgaggatca tgttgaccga
     2101 ggccagttcg tcctcggcct gcaccaggca gccgccgagc ggcggcaggc gcggggccag
     2161 ccattcgagg atctcggtcg ccggcgtgat cggatacgcg gcgacgaagc gcacgccgcc
     2221 gcgcagcgcc ccgagccccg aggcctcgtt gccgctcagc gaccagcgca ccggccggtt
     2281 cgcggcggcg gcgacggccg gcagccgggg cacggccggc agcgccgcga ccacctgcgc
     2341 cccggcgagc gcggcgcgga tgccggactc gaccacggcc tcgcccttgt cggcgagcgc
     2401 cgcggcgagt gttgcgtaca cggcctcggc cggcaggccg atgagttcgg cgagcaggcc
     2461 gagcgcgacc atgttgacgc ggctgccggt ctcgtccgcc agcgcctgca gcgcgacttc
     2521 ggcgcggcgc ggatgcgcgc gcaggatcgc cgccggcagc tcgccggcgg ccggatcgcc
     2581 gatgaccagg ctgtcggcac gcagcggcag ctcggcggcg aagcgctcga tgttctgcca
     2641 gtcgacggcg agcagcacgt cgaaggcatc gtcgggcgcc tcgaccggat gattcgccag
     2701 ccgcagcagc gcggcggcct caccgccgcg gatctgcggg ccgagggccc gcgacatcac
     2761 gccgtacagg ccggcggcgg cggccgcgtc gagcagcatc tgcccggcgg tcatgacgcc
     2821 ggcgccgccg gagccgcaca gcacaatgga aaccgattgc ggattcagtg atgggtccat
     2881 gagtctcgcg acctctcgca gctaaggcaa agccctgaaa tgaaaggcca acccgctggc
     2941 gggtcggccg ctgcggggca tttcagcacc ggcggggcgg cgaaatattg cgctgcgaca
     3001 acttctgccc gtgcgagcgg acgcatccga caaaatgctg atccatatca agaagttgtg
     3061 cgcaccattg cggtgcgcta cgcaccagcg tggggagctg tggcggtgcg ggggatgggc
     3121 a
//
LOCUS       DAOQJH010000321         3121 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_20696_length_3121_cov_3.928245, whole genome shotgun sequence.
ACCESSION   DAOQJH010000321 DAOQJH010000000
VERSION     DAOQJH010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3121
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20696_length_3121_cov_3.928245"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(289..828)
                     /locus_tag="PLQ18_07995"
     CDS             complement(289..828)
                     /locus_tag="PLQ18_07995"
                     /inference="COORDINATES: protein motif:HMM:NF021602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2335 domain-containing protein"
                     /protein_id="HNE01630.1"
                     /translation="MISSASRPTFAKCWTGLAMPTTEETGAGHHDGVRPVTPAVPPAR
                     TPDLPAAVADDLSTINSAAAHMVQVAAQAFSGPLPPPDLLEHYQRIQPDLVERLMRLT
                     ESEAAHRHALETQALAASERIEARGQRYGLLIGLAALAVAGMALSLGHEKAAMTIGGT
                     TVVGLATVFVVGRWKKPSE"
     gene            complement(761..964)
                     /locus_tag="PLQ18_08000"
     CDS             complement(761..964)
                     /locus_tag="PLQ18_08000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE01631.1"
                     /translation="MSKRLHTALRGIGSVLAIAPGPGVAGFRERVRRVRLYRDDREAL
                     RSDFERVAADLRKVLDRIGNADH"
     gene            complement(1489..2583)
                     /locus_tag="PLQ18_08005"
     CDS             complement(1489..2583)
                     /locus_tag="PLQ18_08005"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNE01632.1"
                     /translation="MEARYGFEAAERLARLHAAGLALERLTLALGNAETAAPAAREAA
                     LAASHTAWQQFSLAFSALGAGLAADSTLHHDYAAFERQVASRIAVLAPPDPGPAPGRP
                     PEDRPGEPAEPYRSASARLHAHTAAEAQQLTDAADIHRLRLEHYLQWLGGLLSGSALP
                     ALHLVRRRSADWQQRLRHLEYEATHDPLTQLPNRRLFTDCAVWGLAHALRCQRPACVL
                     LLDLDGFKAVNDRYGHAAGDRLLIDIAARLQQCLRESDLLARLGGDEFAILIPSSCSI
                     GDPDRLAVRLLQSIRTPQPDAAGRVPISASIGIACLPGHGEDIHTLLQAADTAMYAAK
                     RAGGNAFRHAATPAESPPCERAADADSRTA"
     gene            complement(2730..>3121)
                     /locus_tag="PLQ18_08010"
     CDS             complement(2730..>3121)
                     /locus_tag="PLQ18_08010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE01633.1"
                     /translation="GFDVHLHCCYASGPGLGTLGIENPPRGTGLQTLAPALRTVVSGL
                     CRCVFAGALTETEITHGSRRLRLNGLPLLDSNGAISMALIRILPAEPASGARRDTATG
                     FRQRGCDEAADWHGPAGALRQDAAHRR"
BASE COUNT          421 a         1011 c         1216 g          473 t
ORIGIN      
        1 actcataatg ctggggtcct cagttcgatt ctgagcgtag ccaccaaaaa tcaagggctt
       61 aggtgaaaac ctaggccctt gtccttttcg gggtcacgca gggctcaccc tcaagccgtg
      121 actgtgaccc cgggacccgc ggtcatgcgg ctggcgcgtc attctggacg gcgccggcat
      181 gcgggtggcc ggtgctggtt cgatgccgtg ctcgccaccg gcgcgaacgc tgccttccgt
      241 ccggttgccc ggccggtctc gtcatgccga tcagctatgg gatgggtatc actcggacgg
      301 cttcttccag cgtccgacga cgaacaccgt cgccagcccg acaacggttg tgccgcctat
      361 cgtcatcgcg gctttctcgt gaccgagaga cagcgccatg cccgccacgg cgagcgctgc
      421 caggccgatc agcaagccgt accgttgtcc gcgtgcctcg atacgctcgg acgctgcgag
      481 cgcctgcgtt tcgagggcgt ggcggtgtgc ggcttcggat tcggtcaggc gcatcagccg
      541 ctcgaccaga tcgggctgta tgcgctggta gtgttcgagc aggtcgggcg gcggtagcgg
      601 gccgctgaaa gcctgtgctg ccacctgcac catgtgtgcg gcagcgctgt tgatcgtgct
      661 caggtcgtca gcgaccgcgg cgggcaggtc aggagtgcgg gcaggcggta cggcgggcgt
      721 tacaggccgg acgccgtcgt gatgcccggc gccggtttcc tcagtggtcg gcattgccaa
      781 tccggtccag cactttgcga aggtcggccg cgacgcgctc gaaatcactg cgcagcgcct
      841 cgcggtcatc acggtacagg cgcacgcggc gcacgcgctc gcggaagccg gccacgcccg
      901 gaccgggcgc gatggcgagc acgctgccga tgccgcgcag cgcggtatgt aggcgtttgc
      961 tcatggactg cgattccctg ggtggccttc ggggcatggg cgattcatgg acagtatacg
     1021 gtgacggctg gtggcggacg tgccatcgca ggcagcttcc agcctcgcca cccgcgcgca
     1081 gggtgtattc agggccgatg ccatggcgat ccggtcgaag ggtgcccgga atgtgggcgg
     1141 cccggccgca gccggcgttc ccgctgcatg tgccgctcgc gcccggcggg cacatgcagg
     1201 atcaggagtg atccgacacc gggcacggca tccgccggtc cggtgcgcag gcagggccac
     1261 ggatcagggg cggcagggcg caaggtcatt ggctgcaatt tgcacgaggc tgtccaattg
     1321 gcagcgacgt ggcaaatcat ggccaatcgg ccagcctccg ccgtgcgcgt tcgagcggga
     1381 atggcaggtg atggccgtcg catcgccgtc gcgccctgcc gggcacggaa tccgccttct
     1441 ggggccgggc gtttttgcag gggcgccgcg gcgggcatcc gctgcgcttc aggccgtccg
     1501 ggagtcggcg tcggcggccc gttcgcatgg cggggattcc gccggcgtcg cagcgtggcg
     1561 gaaggcgttg ccgcccgcgc gcttggcggc gtacatcgcc gtgtcggcgg cctgcagcag
     1621 ggtgtggatg tcctcgccgt gtccgggcag gcaggcgatg ccgatgctgg cgctgatcgg
     1681 cacccggccg gcggcgtcgg gctgcggcgt gcggatgctc tgcagcaggc gcacggcgag
     1741 ccggtccgga tccccgatgc tgcagctgct cgggatgagg atggcgaatt cgtcgccgcc
     1801 gagccgggcg agcaggtcgg attcgcgcag gcactgctgc aggcgcgcgg cgatgtcgat
     1861 gagcaggcgg tcgccggcgg cgtggccgta gcggtcgttg acggccttga agccgtccag
     1921 atcgagcagc agcacgcagg ccggccgctg gcagcgcagc gcgtgcgcga gtccccagac
     1981 ggcgcagtcg gtgaacaggc gccggttcgg cagctgcgtc agcggatcgt gggtcgcctc
     2041 gtattccaga tgccgcaggc gttgctgcca gtccgccgac cggcgacgga ccagatgcag
     2101 ggcgggcagg gcgctgccgg acagcaggcc gccgagccac tgcaggtagt gctccaggcg
     2161 caggcgatgg atatcggcgg catcggtgag ctgctgcgct tcggccgcgg tatgggcgtg
     2221 cagccgggcg ctggcgctgc ggtatggctc tgcaggttcg ccgggccggt cctcgggagg
     2281 gcgcccggga gccggtccgg ggtcgggcgg cgcgaggacg gcgatgcggc tcgcgacctg
     2341 ccgttcgaag gcggcgtagt cgtggtgcag cgtgctgtcg gcggcaaggc cggcgccgag
     2401 cgcggagaac gcgaggctga actgctgcca ggcggtgtgg ctggcggcga gggccgcttc
     2461 gcgcgcggcc ggcgcggcgg tctcggcgtt gccgagggcg agcgtcaggc gttcgagggc
     2521 gagcccggcc gcgtgcagcc gggcgaggcg ctcggcggcc tcgaaaccgt atcgggcctc
     2581 cacgaccacg agtccgccca gagcggtgag cacaagggcg atgcccgtga cgagcgcgag
     2641 gaagcggcgg cgcggggacc gccggatcgg cacggacgtg cgcagagacg gaagcgctgc
     2701 cggctccggc atcggtacac cgccggcact cagcgtctgt gggcggcatc ctgccggagc
     2761 gcgccggcag ggccgtgcca gtcggcggcc tcgtcgcagc cgcgctggcg gaagccggtg
     2821 gccgtgtccc tccgggcgcc ggaggccggc tcggcgggca ggatgcggat cagggccatg
     2881 ctgatcgcac cattgctgtc gagcagcggc aggccgttca ggcgcagccg ccgcgagccg
     2941 tgcgtgatct cggtttcggt cagcgcaccg gcgaagacgc agcggcacag cccgctcacg
     3001 accgtgcgca gcgccggtgc gagcgtctgc aggccggtgc cgcggggcgg attttcgatg
     3061 ccgagcgtgc cgagtcccgg gccgctggcg tagcagcagt gcaggtgaac gtcgaagccg
     3121 a
//
LOCUS       DAOQJH010000322         3111 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_20800_length_3111_cov_5.582461, whole genome shotgun sequence.
ACCESSION   DAOQJH010000322 DAOQJH010000000
VERSION     DAOQJH010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3111
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20800_length_3111_cov_5.582461"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..846)
                     /locus_tag="PLQ18_08015"
     CDS             complement(<1..846)
                     /locus_tag="PLQ18_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4147 domain-containing protein"
                     /protein_id="HNE01634.1"
                     /translation="MPPESPAGLLRALFDAAVAAAQPALSLAAFLPEAPRGRTVVIGA
                     GKAATTMAQALDAAWPAALSGVVVTRHGQRQPCRRIEVLEAAHPVPDAHSVAAAARML
                     DAVRGLGADDLVICLISGGGSSLLVQPAAGLTLADKQAINRALLASGADIAAMNCVRK
                     HLSAIKGGRLAAACAPARVVTLAISDVPGDEPAVIASGPTVADATSCTDALAILDRYR
                     ITLPPAVRAALEAGELETPKPDDACFARCEYHLIATPMRSLEAAAAVARAAGVTPLIL
                     GDALEG"
     gene            1187..1381
                     /locus_tag="PLQ18_08020"
     CDS             1187..1381
                     /locus_tag="PLQ18_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007508463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HNE01635.1"
                     /translation="MTDTSTARSTRASVSFPPTLYEELVRIAAQKKVSVAWVIREAAE
                     RYVSERYPLFDGRPGSGEHE"
     gene            1378..2061
                     /locus_tag="PLQ18_08025"
     CDS             1378..2061
                     /locus_tag="PLQ18_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006464205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MT-A70 family methyltransferase"
                     /protein_id="HNE01636.1"
                     /translation="MSALPLSDADGDAAADLRRFVSGRSFGTILADPPWQFQNRTGKV
                     APEHRRLTRYGTLTLPEITALPVAEAAAEVCHLYLWVPNALLPEGLQVMAAWGFQYKS
                     NLVWHKIRKDGGPDGRGVGFYFRNVTELILFGVRGKNARTLAPGRSQVNFIASRKREH
                     SRKPDEQYELIEACSPGPFLELFARGERPGWQVWGNQADGEYYPSWDTYAHHSQVRDE
                     PAGVPVTDD"
     gene            complement(2082..2834)
                     /locus_tag="PLQ18_08030"
     CDS             complement(2082..2834)
                     /locus_tag="PLQ18_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018425767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglII/BstYI family type II restriction
                     endonuclease"
                     /protein_id="HNE01637.1"
                     /translation="MLESGFEIRCESHAAAILRIDFPEALEELESSLADIRIPITEII
                     GSGGGEAAGTQRLRRSLAGRGWTKTCFEIRKTLNGVERASISHEIDHVKTFANGTLAL
                     EIEWNNKDPFFDRDLENFKRLHAEGGISAGIIVTRGSRLQQQLRSLVRRYAETRNITD
                     FEALAAVGVSLTPRQRTNISQRLQRSDDPLSAFAEAWAGQFVADKFGAATTHWDKLMT
                     RVQRGVGNPCPLLLIGIAETAVDFNDVAADDP"
BASE COUNT          507 a         1051 c         1052 g          501 t
ORIGIN      
        1 gccttcgagc gcatcgccga ggatcagcgg cgtgacgccg gcagcgcgcg ccaccgccgc
       61 ggcggcttcg agcgagcgca tcggcgtcgc gatcaggtgg tactcgcagc gcgcgaagca
      121 ggcatcgtcc ggcttcggcg tttccagctc gccggcttcg agcgccgcgc gcacggccgg
      181 cggcagggtg atgcggtagc gatcgaggat cgccagcgca tcggtgcagc tcgtcgcatc
      241 cgccacggtc ggcccggagg cgatcaccgc cggctcgtcg ccgggcacgt cggagatcgc
      301 cagcgtcacg acccgcgccg gcgcgcaggc cgcggccagc cgcccgccct tgatcgccga
      361 aagatgcttg cgcacgcagt tcatcgccgc gatgtcggcg ccgctcgcga gcagcgcgcg
      421 gttgatcgcc tgcttgtcgg cgagcgtcag ccccgcggcc ggctgcacca gcagcgacga
      481 gccgccgccg gagatcaggc agatcaccag atcgtcggca ccgagcccgc gcaccgcatc
      541 gagcatgcgc gcagccgccg cgacgctgtg cgcatccggc accgggtgcg cggcttcgag
      601 gacctcgatg cgccggcacg gctggcgctg gccgtggcgc gtgacgacga cgccggacag
      661 ggctgcgggc cacgcggcat cgagcgcctg cgccatcgtc gtcgccgcct tgccggcacc
      721 gatgacgacg gtacgcccgc gcggcgcttc gggcaggaag gcggcgagac tcagcgccgg
      781 ctgggcggcg gcgaccgcgg catcgaacag cgcgcgcagc agtccggcgg gggattcggg
      841 gggcatggag gcggtctccg caggcatctg gaaacggatc ggcgctgcgt accaaccatc
      901 cgcagcgcca gcgcgtgaca agcccggtga taccgatcac atcggaacac accgtctttt
      961 cagcccctcg ccgcttgcgg gagcggggga gaacaacgca tttgcatggg atttggcagg
     1021 agtcgtgcgc ggcaccgcac ggcggagccg gccgcctcgg gagcgggacc cgcgcagccc
     1081 gccgtgcagg ccgcgggatc cgatccgagc gtagccgctg cgggggctgt tcgtgcagca
     1141 aggtggtgta atctggtgca gatgctgcca tccggacccc gccgccatga ccgatacctc
     1201 caccgcccgc agcacccgcg cctcggtgag ctttccgccg acgctctatg aggaactggt
     1261 gcgcatcgct gcgcagaaga aggtgtcggt ggcctgggtg atccgcgagg cggcggagcg
     1321 ttatgtcagc gagcgttatc cgctgttcga cggccgcccg ggcagcggtg agcacgaatg
     1381 agcgcgctgc ctctgagcga tgcagacggc gatgccgcgg ccgacctgcg ccgcttcgtc
     1441 agcggccggt ctttcggcac catcctcgcc gacccgccgt ggcagtttca gaaccgcacc
     1501 ggcaaggtcg cgcccgaaca ccggcggctg acgcgctacg gcacgctgac gctgccggaa
     1561 atcaccgcgc tgccggttgc cgaagccgcg gccgaggtct gtcacctgta tctgtgggtg
     1621 ccgaacgcgc tgctgcccga gggcctgcag gtcatggcgg cctggggctt ccagtacaag
     1681 agcaatctcg tctggcacaa gatcaggaag gacggcgggc cggacgggcg cggtgtcggc
     1741 ttctatttcc gcaacgtcac cgaactgatc ctgttcggcg tgcgcggcaa gaatgcccgc
     1801 acgctcgccc ccggccgcag ccaggtcaat ttcatcgcca gccgcaagcg cgagcattcg
     1861 cgcaagcccg atgaacaata cgagttgatc gaagcctgca gccccggtcc gttcctcgaa
     1921 ctgtttgcgc gcggcgaacg tcccggctgg caggtatggg gcaatcaggc cgatggtgag
     1981 tattacccga gctgggatac ctatgcgcac cattcgcagg tgcgggatga acccgccggc
     2041 gtgccggtca cggacgatta atccggtacg tcatcagcgg attacggatc gtcggctgca
     2101 acatcgttga aatcgacggc cgtctccgca atgccgatca gcagcagcgg acagggattg
     2161 ccgacgccgc gctgcacgcg cgtcatcagc ttgtcccagt gtgtcgttgc cgcaccgaac
     2221 ttgtcggcaa cgaactgacc cgcccaggct tcggcaaagg ccgacagcgg atcatcactg
     2281 cgctgcaggc gctggctgat attggtgcgc tggcgtggtg tcagcgacac acccactgcg
     2341 gccagcgcct cgaaatccgt gatgttccgc gtttcggcgt agcgcctgac gaggctgcgc
     2401 aactgctgct gcaggcgcga tccgcgcgtg acgatgatgc cggcgctgat gccgccttcg
     2461 gcatgcaggc gcttgaagtt ttcgaggtct cgatcgaaga acggatcctt gttattccat
     2521 tcgatttcga gtgcgagcgt gccgtttgcg aaagtcttga cgtgatcgat ctcatgcgaa
     2581 atcgacgcac gctcgacgcc gttcagggtc ttgcggattt caaagcaggt tttcgtccag
     2641 ccgcgcccgg caaggctgcg ccgcaagcgc tgcgtgccgg cggcctcgcc gccgccggag
     2701 ccgatgattt cggtaatcgg aatgcggatg tcggcaagac tcgattcgag ttcttcgagc
     2761 gcctcgggaa aatcgatccg caggatcgct gcagcatggg attcgcagcg gatttcaaaa
     2821 ccggattcca gcaaacgatc aagcatggcg tccgggctgt cccgtgatgc tgctgtcgga
     2881 agtcatcgga tccatcccat gcaccctgtt cccgtgtgtt tccggcaagt tcgtatcgaa
     2941 atctgtccga tcataaacaa acgacccgcc gggatctgca cccgcggcgg gtcgcctgca
     3001 gcgattggtg tcgcgtttca gtgcgccccg gctgccgccg ccccgcccgc cgctccgccc
     3061 tgcttcttcg gccgcgccag ccagacgacg ccgatcagca ccaggaagat c
//
LOCUS       DAOQJH010000323         3090 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_21037_length_3090_cov_5.078089, whole genome shotgun sequence.
ACCESSION   DAOQJH010000323 DAOQJH010000000
VERSION     DAOQJH010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3090
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21037_length_3090_cov_5.078089"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..567
                     /locus_tag="PLQ18_08035"
     CDS             <1..567
                     /locus_tag="PLQ18_08035"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNE01638.1"
                     /translation="EKLDYHAPDLLAARLRADLGTAQATRIIVDAGCGTGLCGLWLKP
                     YAQRLIGIDLSPGMLRKADARGDYDKLYEAELTRFLATYPAAFDVIVSADTLCYFGAL
                     EEFAAAARRSLKAGGWLGFTVERLDDDNPEPYRIRPHGRYAHRGDYVQATLAAAGFSG
                     LSLEPGVLRMELSHPVQGWVVRARVGDA"
     gene            693..968
                     /locus_tag="PLQ18_08040"
     CDS             693..968
                     /locus_tag="PLQ18_08040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE01639.1"
                     /translation="MLRECAAVIAQRIHAAFPARSPGADHGHVLQALLYFRHFRDAGI
                     EVGIGGILHAVLRDALQTRRMDRLTLQPVQRKEAERRHHRQRRVRRP"
     gene            complement(1119..1619)
                     /locus_tag="PLQ18_08045"
     CDS             complement(1119..1619)
                     /locus_tag="PLQ18_08045"
                     /inference="COORDINATES: protein motif:HMM:NF022161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2505 family protein"
                     /protein_id="HNE01640.1"
                     /translation="METLSVSHEIHCSADRFWTTFFDPDFNEQLFVQELGFRDYCVLE
                     QKESENGIECRSTGVIESHLATPLRTLTGQEMRYVQQGCFDRSSRCYRWQSVPARMAD
                     RLRLEGSMRIEPIGTDCVRRIVEFNVEARIFGIGGLLESKVVKELSAECEVSAAAMNR
                     YFEASH"
     gene            complement(2033..>3090)
                     /locus_tag="PLQ18_08050"
     CDS             complement(2033..>3090)
                     /locus_tag="PLQ18_08050"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNE01641.1"
                     /translation="DDEVARVVSELVHQRLRRRTQWRDYAILYRGNHQARPFETALRQ
                     QNVPYRITGGQSFFAQAEVKDVLAYLRLLVNPEDDAAFVRIANVPRRELGPATLEKLA
                     HYAAGRGLALLPACRELGLAQQLPERAAERLAGFAHWLDDVAERARSGEPAAVVREML
                     AELHYEDWLKEQANDPKQGARRMQSVHELTDWLARMGETGDGSAPPSLAERVAKMALI
                     DLLERQEGEEAHDEVTLMTLHAAKGLEFPHVFVVGIEEELLPHRNSIDAGTIEEERRL
                     AYVGITRAQKTLTLTLAQRRRKYGNWESCEPSRFLAELPQADLEWEGAGIELAPEVKK
                     ERGRERLAGLRALLDKP"
BASE COUNT          481 a         1012 c         1056 g          541 t
ORIGIN      
        1 gaaaagctcg actatcacgc gccggatctg ctcgcggcac ggctcagggc cgatctcggc
       61 accgcgcagg ccacgcgcat catcgtcgat gccggctgcg gcaccgggct gtgcgggctg
      121 tggctgaagc cctacgcgca gcgcctgatc ggcatcgacc tgtcgcccgg catgctgcgc
      181 aaggccgatg cccgcggcga ctacgacaag ctgtacgaag ccgagctgac gcgctttctc
      241 gccacctacc cggcggcctt cgacgtgatc gtctcggctg acacgctgtg ctacttcggc
      301 gcgctggagg aattcgccgc tgctgcgcgc cggagcctga aggccggcgg ctggctcggc
      361 ttcacggtcg agcggctgga cgacgacaat cccgagccat accgcatccg cccgcacggc
      421 cggtatgcgc atcgcggcga ctacgtgcag gcgacgctgg ccgcagccgg attcagcggg
      481 ctgtcgctgg agccgggcgt gctgcgcatg gagctgtcac atccggtgca gggctgggtc
      541 gtgcgtgcgc gggtcggcga cgcctgaact cccggccccg ggtccttgtc gcggagaccg
      601 gccacgccgc ccgaggagct gcgccggcag attggcgcct gtctggcgct gcgccatccg
      661 gaaatcgacg aaccggcacg gctgatcgag gaatgcttcg cgaatgcgca gcagtcattg
      721 cgcagcggat tcatgctgcg tttccggccc gaagcccggg cgcagatcac ggtcatgtat
      781 tgcaggcact tttgtatttc aggcactttc gcgatgccgg catcgaggtc ggcatcggcg
      841 gaatccttca cgccgtcctg cgtgatgcct tgcagacccg tcgcatggac aggctgacgc
      901 tgcagccggt gcagcggaaa gaggcggagc ggcggcatca ccggcagcgt cgcgtgcgcc
      961 gaccctgagc atcgtgcgca acgatacgcc gccggagcaa ggctgacccg aagcagccac
     1021 tccgcaatgc cggttggacg gtcgccgtgg cgcggatggc ggatccgcgc cggcagtgcc
     1081 cggaaatcag ggaaaaatga gtgccgggac gaaaggcttc agtggctggc ttcgaaatag
     1141 cggttcatcg ctgcggcgct gacttcgcat tcggccgaca attccttcac aaccttgctt
     1201 tccagcaggc caccgatgcc gaagatgcgc gcctcgacgt tgaattcgac gatgcggcgc
     1261 acgcagtcgg tgccgatcgg ctcgatgcgc atgctgcctt ccaggcgcag gcgatcggcc
     1321 atgcgcgcgg gcacgctctg ccagcggtaa cagcggctgc tgcggtcgaa gcagccctgc
     1381 tgcacgtagc gcatttcctg accggtcagc gtgcgcagcg gcgttgccag atgcgattcg
     1441 atgacgccgg tcgaacggca ttcgatgccg ttttcggatt ccttctgttc cagcacgcag
     1501 taatcgcgaa agccgagctc ctgcacgaac agttgttcgt tgaaatccgg atcgaagaac
     1561 gtcgtccaga aacgatcggc gctgcagtgg atttcgtggc tgaccgaaag ggtttccatg
     1621 attcgctccc tgtcttttgc cggggtgtga atgaggcccc ggggaatggg ccggttcaaa
     1681 gagcaatggt catcgacggc atcgggtgcg ggccgatgcg tttgatggat caggcacgga
     1741 gtttcggaac gaggcgatgc cggccgtccg ggacgccaca ttgatctgta ttgatctatg
     1801 tcaaatcttg caacgatgct ggcagggtaa tcgccagcat tcaggagtca acgcctgtga
     1861 cattacggtt gtgcaagctg ttgaaggcct gcaactccgg tcgcgaaagc agcgctttcc
     1921 ttaaaaaacg gcaatggcaa gcaggcgcga ttccgcccgg aaaaatccgc aggcaggcaa
     1981 atgacctggc cccggtccgc gtcatccgtc gcaaggcaag cgcggggcgc gctcagggct
     2041 tgtcgagcag cgcgcgcagc ccggcgaggc gttcgcggcc gcgctccttc ttcacttcgg
     2101 gggcgagctc gatgccggcg ccttcccatt cgaggtcggc ctgcggcagt tcggcgagga
     2161 agcggctcgg ttcgcagctt tcccagttgc cgtacttcct gcgccgctgc gcgagcgtca
     2221 gcgtcagcgt cttctgcgcg cgggtgatgc cgacgtaggc gaggcgccgc tcctcctcga
     2281 tggtgccggc gtcgatgctg ttgcggtgcg gcagcagttc ctcctcgatg ccgacgacga
     2341 acacgtgcgg gaactccagc cccttggcgg cgtgcagcgt catcagcgtc acctcgtcgt
     2401 gcgcctcctc gccctcctga cgctccagca ggtcgatcag cgccatcttc gcgacgcgct
     2461 cggccagcga gggcggcgcg ctgccgtcgc cggtctcgcc catgcgcgcc agccagtcgg
     2521 tgagttcgtg cacgctctgc atgcgccgcg cgccctgctt cgggtcgttc gcctgttcct
     2581 tcagccagtc ctcgtagtgc agctcggcga gcatctcgcg gaccacggcc gccggctcgc
     2641 cgctgcgcgc gcgttcggcg acatcgtcca gccagtgcgc gaaaccggcg aggcgttcgg
     2701 cggcgcgttc gggcagctgc tgcgcgaggc cgagttcgcg gcaggccggc agcagcgcca
     2761 gcccgcggcc ggcggcgtag tgcgcgagct tttcgagcgt cgccggcccg agttcgcgcc
     2821 gcggcacgtt ggcgatgcgc acgaaggcgg cgtcgtcctc ggggttcacg agcaggcgca
     2881 ggtaggcgag cacgtccttg acctcggcct gcgcgaagaa gctctggccg ccggtgatcc
     2941 ggtacggcac gttctgctgg cgcagcgccg tttcgaaggg ccgggcctgg tggttgccgc
     3001 ggtacaggat cgcgtagtcg cgccactgcg tacgccggcg caggcgctgg tgcacgagtt
     3061 cagagaccac acgtgcgacc tcgtcgtcgc
//
LOCUS       DAOQJH010000324         3090 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_21047_length_3090_cov_2.877100, whole genome shotgun sequence.
ACCESSION   DAOQJH010000324 DAOQJH010000000
VERSION     DAOQJH010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3090
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21047_length_3090_cov_2.877100"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1045
                     /locus_tag="PLQ18_08055"
     CDS             <1..1045
                     /locus_tag="PLQ18_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011551357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HNE01642.1"
                     /translation="AALPGMQRAVGMPDLHPGKVTPIGAAFASVGLIHPALVGNDIGC
                     GMGLWTTELKRAKAKPAALAGRLHGLDGPWDGDTAARLAAAGLAPTVCDAALGTIGGG
                     NHFAELQAIEAIHDAPAVAALGLEAERLLLLVHSGSRGLGEHILRAHVERHGHAPLAA
                     DSDDGRAYLEQHEHARRWAIENRALIAARISDCLRTTGERRLDVCHNSVSAALVDGCA
                     CWLHRKGAAPADAGPVVVPGSRGSFSYLVQPAPDSEATLYSIAHGAGRRWKRSDTRAR
                     LQRRFTADDLTRTALGSEVVCEDRELLYEEAPPAYKDIDTVIRDLVDAGLARVLAVLR
                     PLVTYKLRARVRA"
     gene            1042..1677
                     /gene="prfH"
                     /locus_tag="PLQ18_08060"
     CDS             1042..1677
                     /gene="prfH"
                     /locus_tag="PLQ18_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009150030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HNE01643.1"
                     /translation="MNAAGGWLQLSSGRGPAECGWVIAQLLPKLVAAATAAGLTAKLL
                     DSSPGPLPGTLASALLAVDGEGFEPFARDWTGSVQWIGTSPFRPAHKRRNWFVAIDRL
                     DAPRELPWRADDVRVETLRAGGPGGQHVNRTDSAVRLTHRPSGLQVLAREERSQHQNR
                     RLAEQRLAARLAALARTDRSDTDAERWRCHDRLIRGNARRVFVGPGFDERG"
     gene            1728..2768
                     /gene="rtcA"
                     /locus_tag="PLQ18_08065"
     CDS             1728..2768
                     /gene="rtcA"
                     /locus_tag="PLQ18_08065"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011333398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HNE01644.1"
                     /translation="MSSDFLLIDGAAGEGGGQILRSALSLSMLSGRAFRIERIRAGRE
                     RPVLLRQHLTAVQAAADLCAAEVEGAQAGSQTLAFRPGPIRAGDWHWSIGTAGSCTLV
                     LQTVLPALWHAPAPSRVRIEGGTHNPAAPPADFLQRAWLPLLAGIGLHADLRLLRHGF
                     YPAGGGTIEVTVTPAATLTPLRLEQRGTLRSTGAEALFAALPAGIAERELAEVARRLG
                     WPAESLKLRGLPAERGPGNALLLTLEHEALTEVFTAYGERGVSAETVARRVSNTAARY
                     LASRAAVGPYLADQLLLPLALAGGHFTTVEPSAHTRSNLDVIRQFLPDLRIGFDEIGP
                     DLWRIRAGRQEG"
     gene            2765..>3090
                     /locus_tag="PLQ18_08070"
     CDS             2765..>3090
                     /locus_tag="PLQ18_08070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE01645.1"
                     /translation="MTEPKRRHRPGPHGDDASAAALHQAMPGRGSRMVRMGAQMTRME
                     VPMARMGPQMMRMEVQMARMGAQMTPMEVQVTRMEVQMAPMEAPMARKEVQMTRMEVP
                     MARMET"
BASE COUNT          442 a         1158 c         1107 g          383 t
ORIGIN      
        1 ggctgcgctg cccggcatgc agcgtgcggt cggcatgccc gatctgcacc ccggcaaggt
       61 cacgccgatc ggtgcggcgt ttgcgagcgt cgggctgatc catccggcgc tggtcggcaa
      121 cgacatcggc tgcggcatgg gcctctggac gaccgaactg aagcgcgcga aggcgaagcc
      181 ggcggcgctg gccgggcggc tgcacgggct cgacgggccg tgggatggcg ataccgccgc
      241 gcgcctcgcc gccgcgggac tggcgccgac cgtgtgcgat gctgcgctcg gcaccatcgg
      301 cggcggcaat cacttcgccg agctgcaggc gatcgaggcg atccacgatg ccccggccgt
      361 cgccgcgctc gggctggagg ccgagcgcct gctgctgctc gtgcacagcg gctcgcgcgg
      421 cctcggtgaa cacatcctgc gcgcgcacgt cgagcgccac ggccacgcgc cgctcgctgc
      481 cgacagcgac gacggccggg cgtatctcga acagcacgag cacgcccggc gctgggcgat
      541 cgagaaccgc gcgctgatcg ccgcgcgcat cagcgactgc ctgcgcacca ccggcgagcg
      601 ccggctcgat gtctgccaca acagcgtctc ggccgcgctc gtcgacggct gcgcctgctg
      661 gctgcaccgc aagggcgcag cgcccgccga tgccgggccg gtggtcgtgc cgggttcgcg
      721 cggcagcttc agctatctgg tgcagccggc gcccgacagc gaggcgacgc tgtattcgat
      781 cgcgcacggc gccgggcgcc ggtggaagcg cagcgacacg cgcgcgcggc tgcagcggcg
      841 cttcacggct gatgacctga cccgcaccgc gctcggcagc gaagtggtct gcgaggaccg
      901 cgaactgctc tacgaggaag ccccgcccgc ctacaaggac atcgacacgg tgatccgcga
      961 cctcgtcgat gccgggctgg cgcgcgtgct ggcggtgctg cgcccgctcg tcacctacaa
     1021 gctgcgcgcg cgggtgcggg catgaacgcc gccggtggct ggctgcagct cagcagcggg
     1081 cgcggcccgg ccgaatgcgg ctgggtgatc gcgcaactgc tgccgaagct cgtcgccgcc
     1141 gccacggccg ccgggctgac ggcgaagctg ctcgacagca gccccggccc gctgcccggc
     1201 acgctggcat cggcgctgct cgccgtcgac ggcgaaggct tcgagccgtt cgcgcgcgac
     1261 tggaccggca gcgtgcagtg gatcggcacc agcccgttcc ggccggcgca caagcggcgc
     1321 aactggttcg tcgccatcga ccggctcgat gcgccgcgcg aactgccgtg gcgggccgat
     1381 gacgtgcgcg tcgagacgct gcgcgccggc ggccccggcg gccagcacgt caaccgcacc
     1441 gacagcgccg tgcgcctgac ccaccgcccg agcggcctgc aggtgctggc gcgcgaggaa
     1501 cgctcgcagc accagaaccg ccgcctcgcc gaacaacgcc tcgccgcgcg gctggccgct
     1561 ctcgcccgca ccgatcgcag cgacaccgac gccgaacgct ggcgctgcca cgaccgcctg
     1621 atccgcggca acgcccggcg cgtgttcgtc ggcccgggct tcgacgagcg cggctgacgg
     1681 accggcacgg cacatccggc acggcacata gataaggaac cgatccgatg tcatccgatt
     1741 tcctgctgat cgacggggcc gccggcgaag gcggcggaca gatcctgcgc agtgcgctca
     1801 gcctgtcgat gctgagcggc cgtgccttcc gcatcgagcg catccgcgcc ggccgcgagc
     1861 ggcccgtcct gctgcgccag cacctgaccg ccgtgcaggc tgccgccgac ctctgcgctg
     1921 ccgaagtcga aggcgcgcag gccggcagcc agacgctcgc cttccgcccc ggcccgatcc
     1981 gtgccggtga ctggcactgg tccatcggca ccgccggctc ctgcacgctg gtgctgcaga
     2041 ccgtgctgcc ggcgctctgg cacgcgcccg ctccgagccg cgtgcgcatc gagggcggca
     2101 cgcacaaccc ggccgcgccg ccggccgact tcctgcagcg cgcctggctg ccgctgctcg
     2161 ccggcatcgg cctgcacgcc gatctcaggc tgctgcgcca cggcttctac cccgccggcg
     2221 gcggcaccat cgaagtcacg gtgacgccgg ccgccacgct gacaccgctg cgactcgaac
     2281 agcgcggcac gctgcgcagc accggtgccg aagccctgtt cgcggccctg ccggccggca
     2341 tcgccgaacg cgaactcgcc gaagtggccc gccgcctcgg ctggccggcg gaaagcctga
     2401 agctgcgcgg cctgccggcc gagcgcggcc ccggcaacgc gctgctgctg acgctcgaac
     2461 acgaggcgct gaccgaggtg ttcacggcct acggcgagcg cggcgtcagc gccgaaaccg
     2521 tcgcccgccg cgtcagcaac accgccgcgc gctatctggc cagccgcgcc gcggtcggcc
     2581 cgtatctggc tgatcagctg ctgctgccgc tggcgctggc cggcggccac ttcacgaccg
     2641 tcgaaccctc ggcgcacacg cgcagcaacc tcgacgtgat ccgccagttc ctgcccgatc
     2701 tgcgcatcgg cttcgacgag atcggcccgg acctctggcg catccgcgcg ggcagacagg
     2761 agggatgacg gaaccgaagc ggcgccaccg tcccggcccc cacggcgatg acgcatccgc
     2821 cgccgccttg catcaggcga tgccgggcag gggttcgcgc atggtgcgga tgggtgcgca
     2881 gatgacgcgc atggaagtgc cgatggcacg catgggtccg cagatgatgc ggatggaagt
     2941 gcaaatggcg cgcatgggcg cgcagatgac gccgatggaa gtgcaggtga cgcgcatgga
     3001 agtgcagatg gcgccgatgg aagcgccgat ggcgcgcaag gaagtgcaga tgacgcggat
     3061 ggaagtgccg atggcgcgca tggaaacgtg
//
LOCUS       DAOQJH010000325         3075 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_21226_length_3075_cov_4.140397, whole genome shotgun sequence.
ACCESSION   DAOQJH010000325 DAOQJH010000000
VERSION     DAOQJH010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3075
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21226_length_3075_cov_4.140397"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..764
                     /locus_tag="PLQ18_08075"
     CDS             <1..764
                     /locus_tag="PLQ18_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006788016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent metalloprotease"
                     /protein_id="HNE01646.1"
                     /translation="EFEKAKDKIMMGAERKSMVMSESEKKLTAYHEAGHAIVGRTVPD
                     HDPVYKVSIIPRGRALGVTMFLPEQDRYSYSRQRLESQISSLFGGRIAEVLIFGSDSV
                     TTGASNDIERATEIARNMVTKWGLSDRLGPLTYSEDDGEVFLGRSVTRHKNVSDETAH
                     VIDEEIRSIIDRNYQRAEQILRDHMNALHKMSEALIKYETIDSDQIDDILAGREPRPP
                     QDYADVEPPAAGPGASASNGGKGDAGPIGGPAQQH"
     gene            830..1609
                     /gene="folP"
                     /locus_tag="PLQ18_08080"
     CDS             830..1609
                     /gene="folP"
                     /locus_tag="PLQ18_08080"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013792676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HNE01647.1"
                     /translation="MGVLNVTPDSFSDGGRWIEPAAALEHAYAMVEAGAALIDIGGES
                     TRPGAAEVSEAEELRRVIPLVEHLSAGLAVPVSVDTSKPAVMRAAIAAGAGLINDVRA
                     LRACGALEVVADSQAAVCLMHMQGEPRTMQQAPAYADVVAEVREFLLARAAACLAAGI
                     GSQRIVLDPGFGFGKTLTHNLSLLRHLDALTGGQWPVLVGLSRKSMIGAVLDLPLERR
                     VTGSVAAALLAAVAGARIVRVHDVRETVEALGILRAVRAAP"
     gene            1712..3049
                     /gene="glmM"
                     /locus_tag="PLQ18_08085"
     CDS             1712..3049
                     /gene="glmM"
                     /locus_tag="PLQ18_08085"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020485129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HNE01648.1"
                     /translation="MRYFGTDGIRGRVGEAPVTADFMLKLGWAAGKVLAGAGAQKVLI
                     GKDTRISGYMFESALEAGLSAAGVNIALLGPMPTPAIAYLTRTLRAAAGIVISASHNP
                     YWDNGIKFFARDGRKLPDEIEAAIELELDRPMTTAGPSELGKAERIIDAAGRYIEFCK
                     STVPAALDLRGLHIVIDCANGATYHTAPNVFSEIGARVTVLAASPDGLNINHECGSTR
                     PAALQKAVLEAGADLGIAFDGDGDRVIMVDRHGAVVDGDELLYIIACDRQRMHGLRND
                     AVVGTQMSNLGLELGLRELGIALYRSRVGDRFVMELLSEHGLCVGGESSGHIICLDRT
                     TTGDGIVSALQVLAAMTATGRDLRELLAGMHKCPQVMVNVPVRRRIDWGSCTPVVEAV
                     RATESDLAGRGRVLLRPSGTEPVVRVMVEGDDAARVRRLAHQLAEIVAASAAD"
BASE COUNT          506 a          981 c         1063 g          525 t
ORIGIN      
        1 aggagttcga gaaggccaag gacaagatca tgatgggtgc cgagcgcaag tccatggtca
       61 tgagcgagtc cgagaagaag ctgaccgcct accacgaggc cggtcatgcg atcgtcgggc
      121 gcaccgttcc ggaccacgat ccggtctata aggtcagcat catcccgcgt ggccgcgccc
      181 tcggcgtgac gatgttcctg cccgaacagg accgctacag ctactcgcgc cagcgtctgg
      241 agagccagat ctccagcctg ttcggcgggc gcatcgccga ggtgctgatc ttcggctcgg
      301 attcggtgac caccggtgcc tcgaacgaca tcgagcgggc gaccgagatc gcccgcaaca
      361 tggtcacgaa gtggggtctg tcggaccggc tcgggccgct gacctacagc gaggacgatg
      421 gcgaggtctt cctcggccgt tcggtcacgc gccacaagaa cgtctcggac gagaccgcgc
      481 acgtgatcga tgaggaaatc cgttcgatca tcgatcgcaa ctaccagcgt gccgagcaga
      541 tcctgcgcga tcacatgaac gcgctgcaca agatgtccga ggcgctgatc aagtacgaga
      601 cgatcgattc cgaccagatc gacgacatcc tcgccggtcg ggaaccgcga ccgccgcagg
      661 attacgccga tgtcgaaccg ccggccgccg ggccgggggc ctcggcatcg aatggcggca
      721 agggcgatgc cggcccgatc ggtggtcccg cgcagcagca ctgaggctga ccggtatcgt
      781 gcagttgcaa tgcggttcac gggccctgga tctttcaggg cccgtcgtca tgggggtcct
      841 gaacgtgacc cccgactcct tttcggatgg cggtcgctgg atcgagccgg cagcggcgct
      901 cgaacatgcg tacgccatgg tcgaggccgg tgcggcgctg atcgacatcg gcggtgaatc
      961 aacccggccc ggtgccgccg aggtatccga agccgaggaa ctgcgccggg tcattccgct
     1021 ggtcgagcac ctgagtgccg ggctggccgt gccggtttcg gtcgacacca gcaagccagc
     1081 ggtcatgcgt gccgccatcg cggccggtgc cggcctcatc aacgacgtcc gggccctgcg
     1141 tgcctgtggt gcgctggagg tcgttgccga cagtcaggcc gcggtctgtc tgatgcacat
     1201 gcagggagag ccgcgcacga tgcagcaggc gccggcgtac gcggatgtcg tggccgaggt
     1261 cagggagttt ttgttggccc gcgccgctgc ctgcctggcg gccggcatcg ggtcgcagcg
     1321 catcgtgctt gatcccggct tcggcttcgg caagacgctg acacacaacc tttcattgct
     1381 gcgccacctc gatgcgctga ccggtggaca atggccggtg ctcgtggggc tgtcgcgcaa
     1441 gtccatgatc ggcgccgtgc tcgacctgcc gctggagcgg cgggtcacgg gcagcgtggc
     1501 cgcagcactg ctcgcggcgg tggccggtgc ccgcatcgtg cgggtgcacg acgtgcgcga
     1561 gacggtcgag gcgctgggca tcctgagggc cgtgcgcgct gcaccctgag cgcagcgtcc
     1621 cggtcattcc ggtttttggg tttctggaat ttttggaagc gccagaccag ccgtccggcc
     1681 ggtcgtggcc agactgaata agggtagaca gatgcgttat ttcggtaccg acggcattcg
     1741 ggggcgggtc ggcgaggcac cggtcacggc cgatttcatg ctcaagctgg ggtgggcggc
     1801 cggcaaggtg ctggctggcg ccggtgccca gaaagtcctg atcggcaagg acacgcggat
     1861 ttcgggttac atgttcgaat cggcgctgga agccgggctt tcagccgccg gcgtcaatat
     1921 cgcgctgctc gggccgatgc cgacgccggc catcgcctat ctgacgcgca cgctgcgcgc
     1981 tgccgccggc atcgtcatca gcgcatcgca caatccctac tgggacaatg gcatcaagtt
     2041 cttcgcgcgt gatggtcgca agctccccga cgagatcgag gctgccatcg agctcgaact
     2101 cgatcggccg atgacgacgg ccgggccttc ggaactcggc aaggccgagc gcatcatcga
     2161 tgccgccggc cgctacatcg aattctgcaa gagcacggtg ccagccgcgc tggatctgcg
     2221 tggactgcac atcgtcatcg actgcgccaa cggcgcgacc taccacaccg caccgaacgt
     2281 gttttccgag atcggtgccc gggtcacggt gctggccgcc tcgccggacg gcctgaacat
     2341 caatcatgag tgcggatcga cccggccggc ggcgctgcag aaggcggtgc tcgaagccgg
     2401 ggccgatctc ggcatcgcat tcgatggcga tggcgatcgc gtgatcatgg tcgatcgcca
     2461 cggtgcggtc gtcgatgggg atgaactgct gtacatcatc gcctgtgatc gccagcgcat
     2521 gcatggcctg cgcaacgatg cggtcgtcgg cacgcagatg agcaacctgg ggctcgaact
     2581 cggcctgcgc gaactcggca ttgccttgta ccgttccagg gtcggcgacc gcttcgtgat
     2641 ggagctgctc agcgagcacg gtctgtgcgt cggtggtgaa agctccggac acatcatctg
     2701 tctggatcgc accacgaccg gcgacggcat cgtttcggcc ctgcaggtgc tggcagcgat
     2761 gacggccacc ggccgcgacc tgcgcgagct gctggcaggc atgcacaagt gcccgcaggt
     2821 catggtgaac gtgccggtgc gacgacgcat cgactggggc agctgcacgc cggtcgtcga
     2881 agccgtgcgc gcgaccgaat ccgatctggc cggacgcggg cgcgtgctgc tgcggccttc
     2941 cgggaccgag ccggtcgtgc gcgtcatggt cgaaggcgac gatgccgccc gggtacgccg
     3001 gctcgcgcat cagctcgccg agatcgtcgc cgcgagcgcg gccgactgag tcaggttgct
     3061 ttccggtaag gttat
//
LOCUS       DAOQJH010000326         3071 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_21276_length_3071_cov_3.813329, whole genome shotgun sequence.
ACCESSION   DAOQJH010000326 DAOQJH010000000
VERSION     DAOQJH010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3071
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21276_length_3071_cov_3.813329"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2217
                     /locus_tag="PLQ18_08090"
     CDS             <1..2217
                     /locus_tag="PLQ18_08090"
                     /inference="COORDINATES: protein motif:HMM:NF023326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3418 domain-containing protein"
                     /protein_id="HNE01649.1"
                     /translation="EDGDSLWPANEVPIHRALLAGLLGHTGLRQQEGSEFLGARGRRF
                     RIWPGSGLTKKPPRWLMAAELVETTQVWARTCARIQPEWLETLAAHLIKRAYSEPHWE
                     KRAAQVAATERVTLYGLPVVTGRKVNYGPIDPPLARELFIRHALVNGEFDCRAPFVAH
                     NAALIAEIARIEDKARRRDVLVDEEAIFRFYDARVPERVWSGAAFLKWLKAAGREDPQ
                     LLFLSRETLMAHAAEQVTDARFPDRLVLAGIEFPLAYRFEPGAADDGVTLTVPLAALN
                     QVPAARCEWLVPGLLPERLAALLKTLPKELRRRVVPVPDTVEKLLPELDAHADAEAPL
                     LPALVAALRRLTASEFPEDAFDPAAIPAHLGMNFRVVAPDGAVLAEGRDLRRLRAQFG
                     DAAREAFGTLASAFRAAADKARTPLRARGEPVIPASPAPAPKPAMPAEDPVARLERAE
                     VHDWDFGALAQTIEFVRNGIVLKGWPALVVEGERLAVRVLDTEARAAAAHRHGLHRLI
                     SLRLREPVKYLRRNLPGLAQMGLHYVAVGTQEQLRDDLLAAAIDRAFLVPALAGGGLP
                     RDRAGFEAMLERGRAQFVGCANEVCAQVGQALASFHAVRRRISGQVPLAWMEAAKDMH
                     EQLDALVYPGFVSATPPEWLRRLPVYLKALELRVERLGGRLEQDRQRSAEVQRLWRWY
                     LQRRAQLARRGADDPELERYRWMLEELRVSLFAQELKTLFPVSVKRIEEQGAKVRV"
     gene            complement(2228..>3071)
                     /locus_tag="PLQ18_08095"
     CDS             complement(2228..>3071)
                     /locus_tag="PLQ18_08095"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017678864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HNE01650.1"
                     /translation="PVAYQRLAHPAGELAAARAAAALGAGFVLSTLASVELEAVAALP
                     DAPPLWFQLYCQPERAASVELVQRAEAAGYSALVVTVDAPLAGIRNRERRAGFRLPPG
                     VSAANLRHGQTPFAPGSAQASLVFDGIMRTAPTWRELDWLLDQTRLPVILKGIVSPED
                     AALAIERGVAGLIVSNHGGRVLDTLPASAEALPVIAERVAGRVPILVDGGIRRGTDVL
                     KALALGASAVLIGRPYLHGLAAAGALGVAHVLKMLREELEVAMALTGCARLADIGPGV
                     LWRP"
BASE COUNT          412 a         1079 c         1166 g          414 t
ORIGIN      
        1 gaggacggcg actccctctg gccggccaac gaagtcccta tccaccgtgc gctgctcgcc
       61 ggcctgctcg gccacaccgg cctgcgtcag caggagggca gcgagttcct cggcgcgcgt
      121 ggccggcgct tccgcatctg gccgggatcg ggcctgacga agaagccgcc gcgctggctg
      181 atggctgccg agctggtcga gaccacgcag gtctgggcgc gcacctgcgc gcgcatccag
      241 ccggaatggc tggagacgct ggccgcgcac ctgatcaagc gcgcgtacag cgagccgcac
      301 tgggagaagc gcgccgcgca ggtggcggcg accgagcgcg tgacgctgta cggcctgccg
      361 gtcgtcaccg gccgcaaggt caactacggg ccgatcgatc cgccgctcgc gcgcgagctg
      421 ttcatccgcc acgcgctggt caacggcgag ttcgactgcc gcgcgccgtt cgtcgcccac
      481 aacgccgcgc tgatcgccga aatcgcccgc atcgaggaca aggcgcgccg gcgcgacgtg
      541 ctggtcgacg aggaggcaat cttccgcttc tacgatgcgc gcgtgcccga gcgggtatgg
      601 tcgggggcgg ccttcctgaa gtggctgaag gccgccgggc gtgaggatcc gcaactgctg
      661 ttcctgagcc gcgagacgct gatggcacac gcggccgagc aggtcaccga tgcgcgtttt
      721 ccggatcggc tcgtgctcgc cggcatcgag tttccgctcg cctaccgctt cgagccgggt
      781 gcggccgacg acggcgtgac gctgaccgtg ccgctcgccg cgctcaacca ggtgccggcg
      841 gcgcgctgcg aatggctggt gccggggctg ctgcccgagc ggctggcggc gctgctgaag
      901 acattgccga aggagctgcg ccggcgtgtg gtgccggtgc cggataccgt cgaaaagctg
      961 ctgcccgaac tcgacgcgca cgctgatgcg gaggcgccgc tgctgccggc gctggtcgcg
     1021 gcgctgcgcc ggctcaccgc cagcgagttc ccggaggatg ccttcgatcc ggctgcgata
     1081 ccggcgcatc tgggcatgaa cttccgcgtc gtcgcgcccg atggcgccgt gctcgccgag
     1141 ggccgtgacc tgcgccggct gcgcgcgcag ttcggtgatg cggcgcgcga ggccttcggc
     1201 acgctggcga gcgccttccg ggccgcggcc gacaaggcga ggacaccgct gcgcgcgcgc
     1261 ggcgaaccgg tcattccggc atcgccggca ccggcgccga agccggcgat gccggccgag
     1321 gatccggtcg cgcgtctgga gcgcgccgag gtccacgact gggacttcgg ggcgctggca
     1381 cagaccatcg agttcgtgcg caacggcatc gtgctgaagg gctggccggc gctggtcgtc
     1441 gaaggtgaga gactcgccgt gcgcgtgctc gataccgaag cgcgcgcggc ggccgcccac
     1501 cgccacggcc tgcaccggct gatcagcctg cgcctgcgcg agccggtcaa gtacctgcgc
     1561 cgcaacctgc cggggctcgc gcagatgggt ttgcactacg tggcggtcgg cacccaagaa
     1621 cagctgcgcg acgatctgct cgccgcggcg atcgatcgcg cctttctggt gccggcgctt
     1681 gccggcggcg ggctgccgcg cgatcgggcg ggcttcgagg ccatgctgga gcgcggccgg
     1741 gcgcagttcg tcggctgcgc gaacgaggtc tgcgcgcagg tcgggcaggc gctcgcgagc
     1801 ttccacgccg tgcgccggcg catcagcggc caggtgccgc tcgcgtggat ggaagcggcg
     1861 aaggacatgc acgagcagct cgacgcgctg gtctaccccg gcttcgtcag cgcgacgccg
     1921 cccgaatggc tgcgccggct gccggtatac ctgaaggcgc tggagctgcg cgtcgagcgt
     1981 ctcggcggcc ggctggagca ggaccgccag cgcagcgccg aggtgcagcg gctgtggcgc
     2041 tggtatctgc agcggcgcgc ccagctcgcg cgccgcggtg ccgacgaccc ggagctcgaa
     2101 cgctaccgct ggatgctgga ggaactgcgc gtgtcgctgt tcgcgcagga gctgaagacg
     2161 ctgtttccgg tgtcggtgaa gcgcatcgag gagcagggcg cgaaggtccg cgtctgagcc
     2221 ccgccgctca cggccgccac agcacgcccg ggccgatgtc ggcgagccgc gcgcagccgg
     2281 tcagcgccat cgccacttcg agttcctcgc gcagcatctt cagcacgtgc gcgacgccga
     2341 gcgcaccggc ggcggcgagg ccgtgcagat aggggcggcc gatcagcacg gcgctggcgc
     2401 cgagcgcgag cgccttcagc acgtcggtgc cgcggcggat gccgccgtcg acgaggatcg
     2461 gcacgcgccc ggcgacgcgc tcggcaatca ccggcagcgc ctcggcgctg gccggcaagg
     2521 tatcgagcac gcgcccgccg tggttcgaga cgatcaggcc ggcgacgccg cgttcgatcg
     2581 cgagcgcggc atcttcgggg ctgacgatgc ctttgaggat caccggcagg cgggtctggt
     2641 cgagcagcca gtcgagttcg cgccaggtcg gggccgtgcg catgatgccg tcgaacacga
     2701 ggctggcctg cgcgctgccg ggcgcgaacg gcgtctggcc gtggcgcagg ttggcggcgc
     2761 tgaccccggg cggcagccgg aagccggcac ggcgttcgcg gttgcggatg ccggcgagcg
     2821 gcgcatcgac ggtgaccacc agcgccgaat aaccggcggc ctcggcgcgc tgcacgagtt
     2881 caacactcgc cgcgcgttcg ggctggcagt agagctggaa ccacagcggc ggcgcatcgg
     2941 gcagcgccgc gaccgcttcg agctcgacgc tcgcgagcgt gctcagcacg aagccggccc
     3001 cgagtgctgc cgcggcgcgc gccgcggcca gctcgccggc cgggtgcgcg agccgctggt
     3061 aggcgaccgg c
//
LOCUS       DAOQJH010000327         3066 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_21345_length_3066_cov_4.906011, whole genome shotgun sequence.
ACCESSION   DAOQJH010000327 DAOQJH010000000
VERSION     DAOQJH010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3066
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21345_length_3066_cov_4.906011"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(55..837)
                     /gene="speD"
                     /locus_tag="PLQ18_08100"
     CDS             complement(55..837)
                     /gene="speD"
                     /locus_tag="PLQ18_08100"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006474337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HNE01651.1"
                     /translation="MGFNNLTKTLTFNIYDVCYTETAEEQMAYLGYIDEMYSAARLTD
                     ILSNVANIIGANILNIARQDYQPQGASVTILISEEPIAAGQLSNSTRPGPMPDTVAAH
                     LDKSHITVHTYPESHPENGISTFRADIDVSTCGRISPLKALNYLIHSFDSDIVTLDYR
                     VRGFTRDVEGRKLFIDHKINSIQNYLVQETRDRYQMIDVNVFQENIFHTKMMLKDFDL
                     NNYLFNKDKHDIAPREQVRLESLLRREMAEIFYGKNLRRGLS"
     gene            complement(1005..1421)
                     /locus_tag="PLQ18_08105"
     CDS             complement(1005..1421)
                     /locus_tag="PLQ18_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020558255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HNE01652.1"
                     /translation="MKASVKWLDNMTFVGESGSGHAVIMDAAPEAGGHNLGVRPMEML
                     LIGTGGCSAIDVMLILKKSRQDVTDCRVELDAERAETDPKVFTKIRMHFVVSGRGLSE
                     NHVRRAVDLSAEKYCSASIMLGQVAEVTHSYEIIAV"
     gene            complement(1486..2283)
                     /gene="trpC"
                     /locus_tag="PLQ18_08110"
     CDS             complement(1486..2283)
                     /gene="trpC"
                     /locus_tag="PLQ18_08110"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020503011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HNE01653.1"
                     /translation="MASLPDILKQIIARKHAEVAERSQAVPMVQLAARAAAAPAPRGF
                     TAALREKLNAELPAVIAEAKRASPSKGLLRDPFDPAAIAASYERHGAACLSVLTDRDY
                     FQGHEDYLVAARAACTLPVIRKDFIVDPYQVVEARAIGADCILLIAAALEDEPMRALH
                     DQARGLGMDVLVEVHDAEELERALALDLDMVGINNRNLRSFEVSLDTTLGLLAQIPGD
                     ILVVTESGILERADVARMRAAGVHTFLVGEAFMRAPDPGAKLAELFA"
     gene            complement(2287..>3066)
                     /gene="trpD"
                     /locus_tag="PLQ18_08115"
     CDS             complement(2287..>3066)
                     /gene="trpD"
                     /locus_tag="PLQ18_08115"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008931350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HNE01654.1"
                     /translation="GDVSGSFNVSTAAAIVVAAAGGTVAKHGNRAVSGKTGAADVLEA
                     AGVRLDLTPAQVEQCLAEVGVAFLFAPQYHGAMRHAIGPRREMAVRTIFNVLGPLTNP
                     AGAPHELMGVFSPALVEPLAEVLLRLGCRHVLVVHGEDGLDEISIGAPTLVAEGRDGR
                     VRSYTIAPEDFGLTRAPLDAIRVGSPAESLALIRRVLADEAGPARDIVVLNAGAAIHV
                     AGLAATLAEGVARAQAVIASGAAAGKLDQLVSCTRRLRDTE"
BASE COUNT          467 a          979 c         1094 g          526 t
ORIGIN      
        1 atgaccggca cgacgtactg gatctgaaag caggtctgaa accggccctg cgcctcagga
       61 caggccgcgg cgcaggttct tgccgtagaa gatctcggcc atctcacggc gcagcagcga
      121 ttcgagccgt acctgctcgc gcggcgcgat gtcgtgcttg tccttgttga acaggtagtt
      181 attcagatcg aagtccttca gcatcatctt cgtgtggaag atgttctcct gaaagacgtt
      241 cacgtcgatc atctggtagc ggtcacgggt ttcctgcacc aggtagttct ggatcgagtt
      301 gatcttgtgg tcgatgaaca gcttcctgcc ctcgacatcg cgcgtgaagc cgcgcacgcg
      361 gtaatcgagc gtgacgatgt cggagtcgaa gctgtgaatc aggtagttca gtgccttcag
      421 cggcgagatg cgtccgcagg tcgagacatc gatgtcggcg cggaaggtgc tgatgccgtt
      481 ctccggatgg ctctccggat acgtgtgcac cgtgatgtgg ctcttgtcga ggtgcgcggc
      541 gaccgtgtcc ggcatcggac cgggacgcgt cgagttcgac agctggccgg cggcgatcgg
      601 ctcctcggag atcaggatcg tgacgctcgc gccctgcggc tgatagtcct gacgcgcgat
      661 gttcaggatg ttggcgccga tgatgttggc gacgttgctg aggatgtcgg tcaggcgtgc
      721 ggcgctgtac atctcgtcga tgtagccgag atacgccatc tgctcctcgg ccgtctcggt
      781 gtagcagacg tcgtagatgt tgaacgtcag cgtcttggtg aggttgttga accccatcag
      841 cttcagtcgg ggcagtggct tcggtttcac cgcggctttt cctccgttgc cggccatgtc
      901 gggccgggct ggcttaaaaa aggcgcgagt ataccgaatg ccgatgacaa gtcatcggcg
      961 gcaaaggcat tttggcaccg gcacaacgcc ggcgatccgg ggcttcagac ggcgatgatc
     1021 tcgtagctgt gcgtcacttc ggcgacctga ccgagcatga tcgaggccga gcagtacttt
     1081 tcggccgaca ggtcgacggc gcggcgcacg tggttctcgg acaggccgcg gccgctgacg
     1141 acgaagtgca tgcggatctt cgtgaacacc ttcgggtcgg tttcggcgcg ctcggcgtcg
     1201 agctcgacgc ggcagtcggt cacgtcctga cgcgacttct tcaggatcag catcacgtcg
     1261 atcgccgagc agccgccggt gccgatcagc agcatctcca tcggccgcac gccgaggttg
     1321 tggccgccgg cctcgggcgc ggcatccatg atcacggcgt ggccgctgcc ggactcgccg
     1381 acgaaggtca tgttgtcgag ccacttgacg cttgctttca tcgcagggac ttccgtaggg
     1441 gacgggaacg gggaaaccgg caaggcggcg acggcgcggc cgccctcacg cgaacagctc
     1501 ggcgagcttc gcgcccgggt ccggcgcgcg catgaaggcc tcgccgacca ggaaggtatg
     1561 cacgccggcc gcgcgcatgc gtgccacgtc ggcgcgttcg aggatgccgc tctcggtgac
     1621 gacgaggatg tcgccgggga tctgcgcgag caggccgagc gtggtgtcga gcgagacctc
     1681 gaagctgcgc aggttgcggt tgttgatgcc gaccatgtcc agatcgagcg cgagcgcgcg
     1741 ttcgagctcc tcggcatcgt ggacctcgac cagcacgtcc atgccgagcc cgcgcgcctg
     1801 atcgtgcagc gcacgcatcg gctcgtcctc gagcgcggcg gcgatcagca ggatgcagtc
     1861 ggcgccgatc gcgcgcgcct cgaccacctg atacggatcg acgatgaagt ccttgcggat
     1921 caccggcagc gtgcaggcgg cgcgcgcggc gacgaggtag tcctcgtggc cctggaagta
     1981 gtcgcggtcg gtcagcaccg acaggcaggc cgcaccgtgg cgttcgtagc tcgcggcgat
     2041 cgcggccgga tcgaagggat cgcgcagcag gcccttgctc ggcgaggcgc gcttggcctc
     2101 ggcgatcacc gccggcagct cggcgttgag cttctcgcgc agcgccgcgg tgaagccgcg
     2161 cggtgccggg gcggctgcgg cacgggcggc gagctgcacc atcggcacgg cctggctgcg
     2221 ctcggcgacc tcggcgtgct tgcgggcgat gatctgcttc aggatgtcgg gaaggctggc
     2281 catggactac tccgtgtcgc gcaggcggcg ggtgcaggat acgagctggt cgagttttcc
     2341 ggcggcggcc ccgctggcga tgaccgcctg cgcgcgtgcg acgccttcgg cgagcgtcgc
     2401 ggcaaggccg gcgacgtgga tcgcggcgcc ggcgttcagc acgacgatgt cgcgcgccgg
     2461 gccggcctca tcggcgagca cgcgccggat cagcgccagc gattcggccg gcgagccgac
     2521 gcggatggca tcgagcggtg cgcgcgtcag gccgaagtcc tcgggcgcga tcgtgtagct
     2581 gcgcacccgg ccgtcgcgcc cttcggcgac tagcgtcggg gcgccgatcg agatttcatc
     2641 gagcccgtcc tcaccgtgca cgaccagcac gtgccggcag ccgaggcgca gcagcacctc
     2701 ggcgagcggc tcgaccagcg ccgggctgaa cacgcccatc agctcgtgcg gcgcgccggc
     2761 ggggttggtc agcggcccga gcacgttgaa gatcgtgcgc accgccatct cgcggcgcgg
     2821 accgatcgcg tgcctcatgg cgccgtgata ctgcggcgcg aacaggaagg cgacgccgac
     2881 ctcggcgagg cactgctcga cctgcgccgg cgtcagatcc agccgcacgc cggcggcttc
     2941 gagcacgtcg gccgcgccgg tcttgccgct gacggcgcgg ttgccgtgct tggcgaccgt
     3001 gccgccggca gcggcaacga cgatcgccgc ggccgtcgag acgttgaagc tgccggagac
     3061 atcgcc
//
LOCUS       DAOQJH010000328         3064 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_21361_length_3064_cov_4.459953, whole genome shotgun sequence.
ACCESSION   DAOQJH010000328 DAOQJH010000000
VERSION     DAOQJH010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3064
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21361_length_3064_cov_4.459953"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..388
                     /locus_tag="PLQ18_08120"
     CDS             <1..388
                     /locus_tag="PLQ18_08120"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNE01655.1"
                     /translation="SKPCGRIDREIDDDELVRVGALECRFVSTPGHTPGQGCWQVGGH
                     LFVGDTIFAGSVGRTDFPLSDPGLAQKSLAKLMTLPGELHVHCGHGPDTTLAAELKGN
                     PYLAHVRRALGIKEPRGGYARGGNWG"
     gene            395..1216
                     /locus_tag="PLQ18_08125"
     CDS             395..1216
                     /locus_tag="PLQ18_08125"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005893851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HNE01656.1"
                     /translation="MCELLGMSANVPTDIRFSFTGLMQRGGHTGPHRDGWGIAFYGER
                     GARLFHDPCPSCESEVARLVQQHSIKSRIVISHIRRANRGRVCLENTHPFTRELWGRS
                     WVFAHNGELKGIKRRPCGPYEPIGSTDSEHAFCWLLGRIRERFPQPPRVPATLWRYVA
                     GLAAELNQLGIFNFLLSDSRWLYAHCSTKLAWLTRRAPFGEAQLTDADMAVDFQGETT
                     PDDVVTVIATQPLTGNERWNLLAPGDFIVFDGGEPVWQHGVPAGCATPSAFLHPG"
     gene            complement(1239..1676)
                     /locus_tag="PLQ18_08130"
     CDS             complement(1239..1676)
                     /locus_tag="PLQ18_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016854055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HNE01657.1"
                     /translation="MLRRLAYVCLSVVLAGCASGLSERRARVLASAEALIGTPYRWGG
                     TDRSGLDCSGLVQLSYAEAGLHVPRTTTALLRDGRPAADPEPGDLLLFSENEPGKPTH
                     VGIYAGDGRMIHASSRGGRVLKSSLDTPYWRRNLIGCVRYLDG"
     gene            1784..2266
                     /locus_tag="PLQ18_08135"
     CDS             1784..2266
                     /locus_tag="PLQ18_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007507912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNE01658.1"
                     /translation="MKAASGMVVSMHYTLTDDAGEVLDTSQGRGPFDYLHGHGNIIPG
                     LESRLEGAEPGFKDKVTVAPADGYGERNDQAVFDVPRDQFPPDEEIRVGMQVHGEGPQ
                     GVIAFTVVDVAEDSIRLDGNHPLAGKTLHFDVEILDIRAASEEEIAHGHVHGPHGHHH
                     "
     gene            2314..>3064
                     /locus_tag="PLQ18_08140"
     CDS             2314..>3064
                     /locus_tag="PLQ18_08140"
                     /inference="COORDINATES: protein motif:HMM:NF036516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M61"
                     /protein_id="HNE01659.1"
                     /translation="MNAPIRYTLLATAPHTHRFLSRCTVAEPAADGQCFVLPAWIPGS
                     YLIREFAKNVIAFTACDGDGAPVAVTKTGKDGWRCAPCRGPLSVELEVHALDLSVRTA
                     WLDTTRAYANGTALFPRLPDRADAPCELEVVLPQGETYAGWRVATGLTALATDARGAG
                     LYAAADYDELVDCPIEIGDWQHARFTACGVPHEIVIAGRCTVDLDRLARDLERVCSAQ
                     IRLFGEPAPFERYLFLVMAVGDGYGGLEHRNS"
BASE COUNT          458 a         1108 c         1069 g          429 t
ORIGIN      
        1 ctcgaagccc tgcgggcgca tcgaccgcga gatcgacgat gacgaactcg tgcgggtcgg
       61 cgcgctcgaa tgccgcttcg tgtcgacgcc cggacacacg ccggggcagg gctgctggca
      121 ggtcggcggg cacctgttcg tcggcgacac gatcttcgcc ggctcggtcg ggcgcaccga
      181 ctttccgctc agcgatccgg ggctcgcgca gaagagcctc gcgaagctga tgacgctgcc
      241 cggcgagctg cacgtgcact gcggccacgg cccggacacg acgctggccg ccgagctgaa
      301 gggcaatccc tacctcgcgc acgtgcgccg tgcgctcggc atcaaggagc cgcgcggcgg
      361 ctacgcccgc ggcggcaact ggggctgagc cgcgatgtgc gagctgctcg gcatgagcgc
      421 gaacgtgccg accgacatcc gcttcagctt caccgggctg atgcagcgcg gcggtcacac
      481 cggtccgcac cgcgacggct ggggcatcgc attctacggc gagcgcggcg cgcggctgtt
      541 ccacgacccc tgcccgagct gcgaatccga agtcgcgcgc ctcgtgcagc agcattcgat
      601 caagagccgc atcgtcatca gccacatccg ccgcgccaac cgcggccggg tctgtctgga
      661 gaacacgcat ccgttcacgc gcgagctgtg ggggcgcagc tgggtgtttg cgcacaacgg
      721 cgagctgaag ggcatcaagc gccggccctg cggcccgtat gagccgatcg gcagcaccga
      781 cagcgagcat gcgttctgct ggctgctcgg gcgcatccgc gagcgcttcc cgcagccgcc
      841 gcgcgtgccg gcgacgctgt ggcgctacgt cgccgggctc gcggccgagc tgaaccagct
      901 cggcatcttc aacttcctgc tctccgacag ccgctggctg tacgcgcact gcagcacgaa
      961 gctcgcgtgg ctgacgcggc gcgcgccgtt cggcgaggcg cagctgaccg atgccgacat
     1021 ggctgtcgac ttccagggtg agaccacgcc cgatgacgtg gtcaccgtca tcgccacgca
     1081 gccgctgacc ggcaacgagc gctggaacct gctcgcgccc ggggatttca tcgtgttcga
     1141 cggcggcgaa ccggtctggc agcacggggt gccggccggc tgcgcgacgc cgtcggcgtt
     1201 cctgcatccc ggctgacgaa gcgctcgcgc acggccgctc agccgtcgag atagcggacg
     1261 cagccgatca ggttgcgccg ccagtacggc gtgtcgaggc tcgacttcag cacccggccg
     1321 ccgcggctcg atgcgtggat catgcggccg tcaccggcgt agatgccgac gtgcgtcggc
     1381 ttgccgggtt cgttctcgga gaacagcagc aggtcgccgg gctcggggtc ggcggccggc
     1441 cggccgtcgc gcagcagcgc ggtggtggtg cgcggcacgt gcaggccggc ttcggcgtaa
     1501 ctgagctgca cgaggccgct gcagtcgagg ccgctgcgat cggtgccgcc ccagcggtag
     1561 ggcgtgccga tcagcgcctc ggcactggcg agcacgcgcg cgcgccgttc ggacagcccc
     1621 gatgcgcagc cggccagtac gacggacagg cagacgtacg cgaggcggcg gagcatggcg
     1681 gcggcaccgg aaggcggcag ggcaggggat gcgcccgagt atagccggcg ggcgcctgtg
     1741 ctaaggtcgt gccttcccgt cccctcccag cggtaaaccc tccatgaaag cagcttccgg
     1801 catggtcgtc tccatgcatt acaccctgac cgacgacgcc ggcgaagtgc tcgacaccag
     1861 ccagggccgc ggtcccttcg actacctgca cggccacggc aacatcatcc ccggtctcga
     1921 aagccggctc gaaggtgccg agcccggctt caaggacaag gtcacggtcg caccggccga
     1981 cggctacggc gaacgcaacg accaggccgt gttcgacgtg ccgcgcgacc agttcccgcc
     2041 ggacgaggag atccgcgtcg gcatgcaggt gcacggcgaa ggcccgcagg gcgtcatcgc
     2101 cttcaccgtc gtcgatgtcg ccgaggacag catccgcctc gacggcaacc acccgctggc
     2161 cggcaagacg ctgcatttcg atgtcgagat cctcgacatc cgcgccgcgt ccgaggaaga
     2221 aatcgcccac ggccacgtcc acggcccgca cggccaccac cactgagccg ctgcgaccgc
     2281 ggggccggcc cccggtcgct tcccgatgcc tgcatgaacg caccgatccg ctacacgctg
     2341 ctcgccacgg cgccgcacac ccaccgcttc ctcagccgct gcacggtcgc cgagccggct
     2401 gccgacgggc agtgcttcgt gctgccggcg tggattccgg gcagttacct gatccgcgag
     2461 ttcgcgaaga acgtcatcgc gttcacggcg tgcgacggcg acggtgcgcc ggtggcggtc
     2521 acgaagaccg gcaaggacgg ctggcgctgc gcgccgtgcc gcggaccgct gagcgtcgaa
     2581 ctcgaagtcc atgcgctgga tctctccgtg cgcacggcct ggctcgacac cacgcgcgcc
     2641 tacgccaacg gcacggcgct gttcccgcgg ctgccggatc gcgccgatgc gccctgcgag
     2701 ctggaagtcg tgctgccgca gggcgagacc tacgccggct ggcgcgtcgc gaccgggctc
     2761 acggcgctcg ccaccgacgc gcgcggtgcc ggcctgtacg cggcggccga ttacgacgag
     2821 ctggtcgact gcccgatcga gatcggtgac tggcagcacg cgcgcttcac ggcctgcgga
     2881 gtgccgcacg agatcgtcat cgccgggcgc tgcacggtcg atctcgaccg gctcgcgcgc
     2941 gacctggagc gcgtctgcag cgcgcagatc cggctgttcg gcgagccggc gccgttcgag
     3001 cgctatctgt ttctggtcat ggcggtcggc gacggctacg gcgggctcga acaccgcaac
     3061 tcca
//
LOCUS       DAOQJH010000329         3062 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_21385_length_3062_cov_3.518789, whole genome shotgun sequence.
ACCESSION   DAOQJH010000329 DAOQJH010000000
VERSION     DAOQJH010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3062
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21385_length_3062_cov_3.518789"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..77
                     /locus_tag="PLQ18_08145"
     CDS             <1..77
                     /locus_tag="PLQ18_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013526052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thioredoxin reductase"
                     /protein_id="HNE01660.1"
                     /translation="AVTSAGTGCMAALDAERWLEAGEA"
     gene            321..1247
                     /locus_tag="PLQ18_08150"
     CDS             321..1247
                     /locus_tag="PLQ18_08150"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HNE01661.1"
                     /translation="MSLWPIFDSLAPVFLLIALGAALRRSGFLSLTTLTGLNRLTYWI
                     GLPCLTVHDIATGPFQPLAAAGLFTAVLAATVIGGVLAWYCAGACGAGRAARGTFVQA
                     AFRGNLYFISLPVIVYASPGHPEVRSAALIASAPVILLYSIAAVLVLEASRSTPGAGR
                     AGLWRSLATNPQLLACVIGLALAASGAGLPPSLERSLDALGRMALPLALLGIGGSLIG
                     AHIGSRLRPVFGAALIKTALLPLLGFGFGWLLGLAPEQLRSALILLSTPTASASYVLA
                     RQLGGDEALASGSIVLSTLLSAPVLALVLALV"
     gene            1524..2873
                     /gene="chrA"
                     /locus_tag="PLQ18_08155"
     CDS             1524..2873
                     /gene="chrA"
                     /locus_tag="PLQ18_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018152784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HNE01662.1"
                     /translation="MPPAPDRSDALTAPPAPGFGEALRYWLRLGCISFGGPAGQIAIL
                     HQDLVEHRRWISESRFLHALNYCMVLPGPEAQQLATYIGWLLHGTKGGIAAGALFVLP
                     SVFVLIALSWIYLAFGQVPAVAGVLWGIKPAVTAIVVFAAWRIGSRALKNPVLWAIAA
                     AAFLGIAVFDLPFPLIVIGAGLIGALGGRLAPQCFRGGGGHGAGKASYGPALIDDHTP
                     TPAHARIDRGRLTRLALGGFGLWAGGLGLLALVFGGVLVDMGLFFTKAALLTFGGAYA
                     VLPYVYQGAVEHFHWLTGAQMIDGLALGETTPGPLIMVVAFVGYVGAWTKEVLGPDAL
                     AWAGALGAVVATVFTFLPSFLFILIGGPWVEATRGDLKFTAPLTGITAAVVGVILSLA
                     LFFAEHVLWPAGFGGRFEWLAAVIGAGALVALIRYKAGVIPVIGVCAGLGLLHGLVG"
BASE COUNT          312 a         1119 c         1113 g          518 t
ORIGIN      
        1 aggccgtgac ctcggccggc accggctgca tggccgcgct cgatgccgag cgctggctgg
       61 aggccggcga agcctgagct tccccgggct gcgtgcgccg caccgcgggc ggcagcgcgg
      121 cccgatgagc gcggggccgg tgcagcagcc ggtgtgcgcc gacggacggg atgtcgtcgg
      181 cgcgtacctc cgcggtttcc ggccgggggc tttcgccgcc cgcccgttcc cgctgcgcgc
      241 atgcgccggg tcacactggg gccggcgtcg ccgcgcccgg ggccggcgca gcgccttgcg
      301 tcgtaccggg agcgcccgtc atgagcctct ggccgatctt cgattcgctg gcgccggtct
      361 tcctgctgat cgcgctcggc gccgcgctgc ggcgcagcgg cttcctgtcg ctgacgacgc
      421 tgaccggcct caaccggctg acctactgga tcggcctgcc ctgcctgacc gtgcacgaca
      481 tcgccaccgg cccgttccag ccgctggcgg cggccgggct gttcacggcc gtgctggccg
      541 cgaccgtgat cggcggcgtg ctggcctggt attgcgccgg ggcctgcggg gccgggcgcg
      601 ccgcgcgcgg caccttcgtg caggcggcgt ttcgcggcaa cctgtacttc atcagcctgc
      661 cggtgatcgt ctacgcctcg cccggccacc cggaggtgcg cagcgccgcg ctgatcgcct
      721 cggcgccggt gatcctgctc tacagcatcg ccgcggtgct ggtgctcgaa gccagccgca
      781 gcacgcccgg cgccggccgc gccgggctct ggcgcagcct cgcgaccaat ccgcaactgc
      841 tcgcctgcgt catcggcctc gcgctcgcgg ccagcggggc cggactgccg ccgagtctgg
      901 agcgcagcct cgatgcgctc gggcgcatgg cgctgccgct ggcgctgctc ggcatcggcg
      961 gctcgctgat cggtgcgcac atcggcagcc ggctgcggcc ggtgttcggg gcggcgctga
     1021 tcaagaccgc gctgctgccg ctgctcggct tcggcttcgg ctggctgctc ggactcgcgc
     1081 cggagcagct gcgctcggcg ctgatcctgc tgtcgacgcc gaccgcgtcg gcctcctacg
     1141 tgctcgcgcg ccagctcggc ggcgacgagg cgctcgcctc gggcagcatc gtgctgtcga
     1201 cgctgctgtc ggcgccggtg ctggcgctgg tgctcgcgct ggtctgaggg cgtcttgccg
     1261 gagcccgcga ttcagccgcg ctatacctcc gggagaggat tttggggtaa gggcttcccg
     1321 gccccccgga gcgaccccgc cagtcgtggt tccggaccgg acagcagcgc ggcgccgggt
     1381 gtcgcactgg gcagtccgtg gccgcttcgg caagctgtcg ggccaacgac atgtcggaag
     1441 ccgccaccgc ggtcgggggc cgccggaatc gttcttctcc cagactgccc gcaggtatcg
     1501 cgatgactgc ccccgaaccc ctcatgcccc cggcgccgga ccggtccgat gcgctgacag
     1561 caccgccggc gcccggcttc ggcgaggcgc tgcgctactg gctgcgcctc ggctgcatca
     1621 gcttcggcgg gccggccgga cagatcgcga tcctgcatca ggatctggtc gaacaccggc
     1681 gctggatcag cgaatcgcgc ttcctgcacg cgctgaacta ctgcatggtc ctgcccggcc
     1741 ccgaggcgca gcagctcgcg acctacatcg gctggctgct gcacggcacg aagggtggca
     1801 tcgccgccgg cgcgctgttc gtgctgccct cggtgttcgt gctgatcgca ctgagctgga
     1861 tttacctggc cttcggtcag gtgccggccg tggccggcgt gctgtggggc atcaagccgg
     1921 cggtcaccgc gatcgtcgtg ttcgcggcct ggcgcatcgg ctcgcgggcg ctgaagaacc
     1981 ccgtgctgtg ggccatcgcc gccgcggcct tcctcggcat cgccgtgttc gatctgccgt
     2041 ttccgctgat cgtgatcggc gccggcctga tcggggcgct cggcgggcgg ctggcgccgc
     2101 agtgcttccg cggcgggggc ggccacggcg ccggcaaggc gagctacggc ccggcgctga
     2161 tcgatgacca cacgccgacc ccggcgcacg cgcgcatcga ccgcgggcgg ctcacgcgcc
     2221 ttgcgctcgg tggcttcggg ctctgggccg gcggactcgg gctgctggcc ctcgttttcg
     2281 gcggcgtgct ggtcgacatg ggcctgttct tcacgaaggc ggcgctgctg accttcggcg
     2341 gtgcctacgc ggtgctgccg tacgtctatc agggcgcagt cgagcacttc cactggctca
     2401 ccggcgcgca gatgatcgac ggcctcgcgc tcggcgagac cacgccggga ccgctgatca
     2461 tggtcgtcgc cttcgtcggc tacgtcggtg cctggaccaa ggaggtcctc gggccggacg
     2521 ccctggcctg ggccggcgcg ctcggcgccg tggtcgcgac cgtgttcacg ttcctgccgt
     2581 ccttcctgtt catcctgatc ggcggcccct gggtcgaggc gacgcgcggg gatctcaagt
     2641 tcacggcccc gctgaccggc atcaccgccg cggtggtcgg cgtgatcctg agcctggcgc
     2701 tgttcttcgc cgagcacgtg ctgtggccgg ccggcttcgg cgggcgcttt gaatggctcg
     2761 cggccgtgat cggcgccggc gcgctggtgg cgctgatccg ctacaaggcc ggcgtgattc
     2821 cggtgatcgg cgtctgtgcg gggctcgggc tcctgcacgg gctggtgggc tgaactccgc
     2881 agcgcgtgac gcagacggca gccggccgcc gcctgttttg cacctgtccg gatgcagaac
     2941 ggaaattgct gacgcttcgg cctcggctcg acgttgagcg tcggtcacac gacacttgac
     3001 gaccgtttcc ccccgtccgg agtcccgcct cgatgagcct gccgctcgcg atcctcgtgc
     3061 tg
//
LOCUS       DAOQJH010000330         3047 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_21560_length_3047_cov_3.585227, whole genome shotgun sequence.
ACCESSION   DAOQJH010000330 DAOQJH010000000
VERSION     DAOQJH010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3047
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21560_length_3047_cov_3.585227"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(240..668)
                     /locus_tag="PLQ18_08160"
     CDS             complement(240..668)
                     /locus_tag="PLQ18_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018280879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HNE01663.1"
                     /translation="MQSFTYPVALTADEHDGGFVVTCRDIPEAITQCESPADCLEEAE
                     GALQAAVEARIMDDLDIPPASEPVHGEIAVSLPVTTALKAALYLSMRDAGLSKSALAR
                     RLGIDEKEARRMLTPGHPTKAVVLERALHALGKRIEIKVA"
     gene            complement(679..1083)
                     /locus_tag="PLQ18_08165"
     CDS             complement(679..1083)
                     /locus_tag="PLQ18_08165"
                     /inference="COORDINATES: protein motif:HMM:NF019540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HNE01664.1"
                     /translation="MPAAQGCAHAAQAFAHARHLHLPALHASIDACSAGIDACATAVA
                     VRSKMTDAAVHVMATPEARAMKGSEFIHKVRRYAKASGLAFEWRPELGKGSHGVLILG
                     GRRTVVRNLKDELKTGTLHAMLRQLGIAADDL"
     gene            complement(1146..>3047)
                     /locus_tag="PLQ18_08170"
     CDS             complement(1146..>3047)
                     /locus_tag="PLQ18_08170"
                     /inference="COORDINATES: protein motif:HMM:NF014871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HNE01665.1"
                     /translation="AAPVAEELTLFHVGDQESWLISAQGNRYDTPDDPISFYGGAARL
                     SALLQQQRAAALAAGRAVLVLNAGDSFLPGPRLTASLKHLAQAAPGGGQDFYDAIVLR
                     RLGFTAVCFGNHEFDLGPDVTAQFVAVAGTPYLSANLDFSGHAALQALVAEGRIAKTL
                     IVDTPKGNRIAVIGATTPRLPDISSPGAVTVRDRDPAADEDTKLLALAPLLQAEIDRA
                     RSAGASVVVLLSHLQGYRNELKLVPKLSGLDVFVSGGGHELMTSPGMATILDEPAMIR
                     GYPQVVALEDGSRVLGVTGNFGNRYVGELQLSLDAQGRVRRDADGVPLTGTGSRMHRV
                     SGNPADADHVLPDPVIEAGVVQPVRAFIAALSEQKIATTPVELNGVRGSAAESGAQAQ
                     YGVRNSETNLGNLVADAVRFAAGTDLAIQNGGGIRTSIRAGEVSAGDVFNVLPFTNLI
                     VQFRGLDAGQLRILMEHALANASPDGAASGRFAQVSGLRIVYDSRRPPLQLDADGRPA
                     GGGRILSMTLDDGTQLVEDGIVRTGVRPLSLATIDFTARGGDGYPFARLGLTPEPLVH
                     TIHYQEALLDYLSQPADKGGLGGVIDTRRYGPVDPQAGGGRLIDRALLKPVSGLSGRV
                     SVGRREAAV"
BASE COUNT          475 a         1080 c         1010 g          482 t
ORIGIN      
        1 agacgctgat ggcgaatccg catacaccgg aactggcgca gttcatcggg gcggtagcgg
       61 cggcgtaaag ggttgatacc gatcacggct gaacacgtca gtctttcagc ccctcgcccc
      121 tcgcgggaga ggggtaggaa agcgggagga aaacaacgca tttacgtgtg atcgatatga
      181 gattgccaat tccagagccc ttttcaccga accgcaccag gccggacctc cgcccgccgc
      241 tacgccacct tgatctcgat gcgcttgccg agagcgtgca gcgcgcgctc cagaacaacg
      301 gccttcgtcg gatggcccgg agtcagcatg cgccgcgctt ccttctcgtc gatgccgagt
      361 cgtcgggcga gcgccgactt gctgagcccg gcatcgcgca tcgagaggta cagcgcggct
      421 ttgagcgccg tcgtgaccgg cagggatacc gcaatctctc cgtgcacagg ctcgctcgct
      481 ggcgggatgt ccagatcgtc catgatgcga gcctcgacgg cggcctgcag ggcgccttcg
      541 gcttcttcca ggcaatccgc cgggctttcg cactgcgtga tcgcttcggg gatgtcccgg
      601 caggtcacga cgaacccgcc atcgtgctcg tcggccgtga gtgctacggg gtaggtgaag
      661 ctctgcatga tggcctcctt acagatcgtc cgccgcgatg cccagttgcc tgagcatggc
      721 gtgcaaagtg cccgtcttga gttcgtcctt gaggttgcgc acgaccgtgc gccgtccgcc
      781 gaggatcagc acgccgtggc tgcccttgcc aagctcgggc cgccactcga acgcgagccc
      841 actggctttt gcgtaccgcc ggaccttgtg gatgaactcg ctgcccttca tggccctggc
      901 ttccggcgtt gccattacat gcactgcagc gtcggtcatt tttgaccgaa cagcaaccgc
      961 tgtggcacat gcatcgatcc ctgcgctgca tgcatcgatc gatgcatgca gcgccggcaa
     1021 gtgcagatgc cgcgcatggg cgaatgcctg cgcggcatgg gcgcaccctt gcgccgcagg
     1081 catcgaccca tccacggcaa gtgcggaccc ttcggccggc agggccgatg ctcactcgtc
     1141 agggatcaaa ccgccgcctc gcgcctgccg acgctcaccc gccccgacag ccccgacacc
     1201 ggcttcagca gcgcccgatc gatcagcctg cccccgcccg cctgcggatc gaccggcccg
     1261 tagcgcctcg tgtcgatcac gccgccgagc ccgcccttgt ccgccggctg actcaggtag
     1321 tcgagcagcg cctcctgata atggatcgtg tgcaccagcg gctccggcgt cagcccgagg
     1381 cgtgcgaacg gatagccgtc gccaccgcgg gcggtgaagt cgatcgtcgc gagcgacagc
     1441 ggccggacac cggtgcggac gatgccgtcc tcgaccagct gcgtgccgtc atcgagcgtc
     1501 atcgacagga tgcgtccgcc gcccgcaggc ctgccgtcgg catcgagctg cagcggcggg
     1561 cggcgcgagt cgtagacgat gcgcaggccc gagacctgcg caaagcgccc gctcgcggcc
     1621 ccgtccggcg aggcattcgc cagcgcatgc tccatcagga tgcggagttg cccggcgtcc
     1681 agcccgcgga actgcacgat caggttcgtg aacggcagca cgttgaacac gtcgccggcg
     1741 ctcacctcgc cggcccggat cgaggtgcgg atgccgccgc cgttctggat cgcgaggtcg
     1801 gtgccggcgg cgaagcgcac ggcgtcggcg accaggttgc cgaggttggt ctcgctgttg
     1861 cgcacgccgt actgcgcctg cgcgccggac tccgccgccg agccgcgcac gccgttgagt
     1921 tcgacggggg tcgtggcgat cttctgctcg ctcagcgccg cgatgaacgc ccgcaccggc
     1981 tgcacgaccc cggcctcgat gaccggatcg ggcaggacgt gatcggcatc ggccgggttg
     2041 ccgctgacgc ggtgcatgcg gctgccggtg ccggtcagcg gcacgccgtc ggcatcgcgc
     2101 cgcacccgcc cctgcgcatc gagcgacagc tgcagttcgc cgacgtagcg attgccgaag
     2161 ttgccggtga cgccgagcac gcggctgcca tcctcgagcg cgacgacctg cggatagccc
     2221 cggatcatgg ccggctcatc gaggatcgtc gccatgccgg gcgaagtcat cagctcgtgg
     2281 ccgccgccgg agacgaacac gtcgaggccg ctgagcttcg gcaccagctt cagctcgttg
     2341 cggtagccct gcagatggct gagcagcacg acgacgctcg cgcccgccga gcgcgcgcgg
     2401 tcgatctcgg cctgcagcaa gggcgcgagt gccagcagtt tcgtgtcctc atccgcggcc
     2461 gggtcacggt cacgcaccgt gaccgccccg ggcgaggaga tgtccggcag ccgcggcgtg
     2521 gtcgcaccga tcacggcgat ccggttgccc ttcggcgtat cgacgatcag ggtcttcgcg
     2581 atccgtcctt ctgcgaccag cgcctgcagc gcggcatggc cggagaaatc gaggttggcc
     2641 gacagatacg gcgtgccggc caccgcgacg aactgcgcgg tcacgtccgg gccgaggtcg
     2701 aactcgtgat tgccgaagca gacggccgtg aagccgagcc ggcgcagcac gatggcatcg
     2761 tagaaatcct gcccgccccc cggggcggcc tgcgcgagat gcttcaggct cgccgtcagc
     2821 cgcggccccg gcaggaagga atccccggca ttgagcacca gcaccgcccg ccctgccgcc
     2881 agcgctgccg cacgctgctg ctgtagcagc gccgaaagcc gcgcggcccc gccgtagaag
     2941 ctgatcggat catccggcgt gtcgtaccgg ttgccctgcg cactgatcag ccaggattcc
     3001 tgatcaccga cgtggaacag cgtcagttcc tcggccaccg gcgcggc
//
LOCUS       DAOQJH010000331         3046 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_21570_length_3046_cov_5.026747, whole genome shotgun sequence.
ACCESSION   DAOQJH010000331 DAOQJH010000000
VERSION     DAOQJH010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3046
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21570_length_3046_cov_5.026747"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..915)
                     /locus_tag="PLQ18_08175"
     CDS             complement(<1..915)
                     /locus_tag="PLQ18_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002690706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HNE01666.1"
                     /translation="MLSRVAQNLYWLARYVERAEDTARLIGVTSNLLLDLPRTAGAAE
                     WEPLIYITGSHEGFREHFTRPDEVSIVSFLTGDLRNSGSILSSLTYARESLRTTRDVM
                     PREAWEELNRIYLFVRDHISDGVSPRGRDYFLKQVVSGCQRFNGYLEGCMSYTPARDF
                     VLLGRYLERADMTTRILDVRSGNLLTQSGDLSPYASIQWMSVLRSLSGYQMYRQAVRS
                     RVRGPDVLAFLLQDTRFPRTVRHCLDRIADCLRELPQPETALAQIARACAKTESADVE
                     ALVTTGLHEFIDELQVELGEVHNAINAAY"
     gene            complement(919..2376)
                     /locus_tag="PLQ18_08180"
     CDS             complement(919..2376)
                     /locus_tag="PLQ18_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019952341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HNE01667.1"
                     /translation="MSMLDWQQYPAGAFYDELVAPGGTPRQAARTLCEHLGKLSDAEL
                     TERRTAAELAISVMGITFTVYSEGNMIDRAWPFDIVPRIIPLTEWTRIEAGLKQRVQA
                     LNLFIDDLYHEQKVIRDGILPAEILAQSVNFRPQCVGITPPHRVWAHICGSDLVRDKD
                     GTVYVLEDNLRVPSGVSYMLENRVITKRVLPELFKTSRILPVDDYPAQLFEMLSSMSP
                     RPGEDPRIVVLTPGIYNSAYFEHAYLAQQMGVQLVEGPDLFVDDDDCCYMRTIYGPQR
                     VDVIYRRIDDLFLDPEAFIPDSMLGVRGLLRSWRAGKVGLANAPGAGVADDKVVYAFV
                     PELIKYYLDQDAILSNVPSYLCMRDKDRDYVLANLDKLVVKPANESGGYGMLVGPHST
                     PEKRAQFAELIKADPRNYMAQPTLAISTSPTLCDGNAAEPRHVDLRPFILSGPKPYVT
                     TGGLTRVALVKGSLVVNSSQGGGSKDTWIVDTEET"
BASE COUNT          489 a          962 c         1067 g          528 t
ORIGIN      
        1 gtacgcggcg ttgatggcgt tgtggacttc gccgagttcg acctgcagct cgtcgatgaa
       61 ctcgtgcagg ccggtggtca cgagcgcctc gacatcggcc gattcggtct ttgcgcaggc
      121 ccgtgcgatc tgtgcgagcg cggtttcggg ctgcggcagt tcgcgcaggc agtcggcgat
      181 gcggtccagg cagtggcgca cggtgcgcgg gaagcgcgtg tcctgcagca ggaaggcgag
      241 cacgtccgga ccgcgcacgc gcgagcgcac ggcctgacgg tacatctggt agcccgacag
      301 cgaacgcagc acgctcatcc actggatgct cgcgtacggc gacaggtcgc cgctctgcgt
      361 cagcaggttg cccgagcgca cgtcgaggat gcgcgtggtc atgtcggcgc gttcgaggta
      421 gcgcccgagc agcacgaagt cgcgcgccgg cgtgtagctc atgcagcctt cgaggtagcc
      481 gttgaagcgc tggcaaccgc tgacgacctg cttcaggaag tagtcgcggc cgcgcgggct
      541 gacgccgtcg gagatgtggt cgcgcacgaa cagatagatg cggttgagct cctcccaggc
      601 ctcgcgcggc atcacgtcac gggtcgtgcg caggctctcg cgggcgtagg tcagcgagct
      661 caggatcgag ccgctgttgc gcaggtcacc ggtcaggaag ctgacgatgc tgacttcgtc
      721 cgggcgggtg aagtgctcgc ggaaaccctc gtggctgccg gtgatgtaga tcagcggctc
      781 ccactcggcg gcaccggccg tacgcggcag atcgagcagc aggttgctgg tcacgccgat
      841 caggcgcgcg gtgtcctccg cgcgctcgac gtagcgggcc agccagtaga ggttctgggc
      901 gacgcgcgaa agcatggatc aggtctcctc ggtatcgacg atccaggtgt ccttgctgcc
      961 gccaccctgg gaggaattga cgacgagcga acccttgacc agcgcgacgc gggtcaggcc
     1021 gccagtggtc acgtagggct tgggaccgga caggatgaac gggcgcaggt cgacgtggcg
     1081 cggctcggcg gcgttgccgt cgcagagcgt cgggctggtc gagatcgcca gcgtcggctg
     1141 cgccatgtag ttgcgcggat cggccttgat cagctcggcg aactgcgcgc gcttctcggg
     1201 cgtcgagtgc ggtccgacca gcatgccgta gccgccggac tcgttggccg gcttgaccac
     1261 cagcttgtcg aggttggcga gcacgtagtc gcggtccttg tcgcgcatgc acaggtagct
     1321 cggcacgttc gacaggatcg cgtcctgatc gaggtagtac ttgatcagct ccggcacgaa
     1381 ggcatagacg accttgtcat cggccacgcc ggcgcccggg gcgttggcga gcccgacctt
     1441 gccggcacgc cacgagcgca gcaggccgcg cacgccgagc atcgagtcgg ggatgaaggc
     1501 ctcgggatcg aggaacaggt cgtcgatgcg ccggtagatc acgtcgacgc gctgcggccc
     1561 gtagatcgtg cgcatgtagc agcagtcgtc gtcatcgacg aacagatccg gcccctcgac
     1621 cagctgcacg cccatctgct gcgcgaggta ggcgtgttcg aaataggccg agttgtagat
     1681 gccgggggtc agcacgacga tgcgcggatc ctcgccgggg cgcggcgaca tcgagctcag
     1741 catctcgaag agctgcgccg ggtagtcatc gaccggcagg atgcggctgg tcttgaacag
     1801 ctcgggcagc acgcgcttgg tgatcacgcg gttctcgagc atgtacgaga cgccggaggg
     1861 cacgcgcagg ttgtcctcca gcacgtagac ggtgccgtcc ttgtcgcgca cgaggtcgga
     1921 gccgcagata tgcgcccaga cccggtgcgg cggcgtgatg ccgacgcact gcggccggaa
     1981 gttcaccgac tgcgcgagga tctcggccgg caggatgccg tcccggatga ccttctgctc
     2041 gtgatacagg tcgtcgatga acaggttcag ggcctgcacg cgctgtttca ggccggcctc
     2101 gatgcgcgtc cactcggtca gcgggatgat cctcggcacg atgtcgaacg gccaggcccg
     2161 gtcgatcatg ttgccttcgg aatagaccgt gaacgtgatg cccatgacgc tgatcgccag
     2221 ttcggcagcg gtgcgccgct cggtcagctc ggcatcggag agcttgccga ggtgctcgca
     2281 gagcgtgcgc gccgcctgcc gcggcgtacc gccgggcgcg accagttcgt catagaaggc
     2341 ccctgcgggg tattgctgcc agtcaagcat gctcatgatg gtgtgattcc ttgtgccggg
     2401 cgccgttgaa tgcggcgccg cctgcgacgc gcccccgaac tgcctctccc cggggggcgc
     2461 aacgactact tcaacagcaa gccccgtacc actcgccggg ctccgctgtc cgggatcgcg
     2521 gcagacggtg ccggcggctc agccctgccg gtcccgccgg acgtgcggca ggggccggac
     2581 gctcgcagcg ccatgatggg gcaggggttt agaccaaaag gccggtgagg cggctggttt
     2641 tgacggataa gcggctcgga catggcgttg tgcatttgat tggtgcgtcc tgcacggcaa
     2701 attgcacttc attggtgcgt gagggcaagg actgtcggat caccggcgga cttcgcagag
     2761 gcattcgcct ggctttggcc ccgcctgtct gccggatgct ccggaccgcg taattgtcac
     2821 ggactggaac gccggttgct agtacacttc gcgcccggcc gttgccgtga gagcccaggg
     2881 aacttccccc gatgacaatc cgcgttgcac tgaaccacaa gacttcttat cacttcgatc
     2941 gcccggtatc gctgtcgccg cacatcgtca ggctgcgtcc cgcgccgcat gctcgcacac
     3001 cgatcccggc gtattcgctg aagatcaatc cgcagccgca cttcat
//
LOCUS       DAOQJH010000332         3035 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_21710_length_3035_cov_3.189597, whole genome shotgun sequence.
ACCESSION   DAOQJH010000332 DAOQJH010000000
VERSION     DAOQJH010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3035
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21710_length_3035_cov_3.189597"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..873
                     /locus_tag="PLQ18_08185"
     CDS             <1..873
                     /locus_tag="PLQ18_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015433853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HNE01668.1"
                     /translation="EEGSWGLGPLAQRRFRLYLPPGATRARPVPVLLLLHGCGQDSAS
                     FAASTHVARLAQREQFAVLMPEQTQQANAQRCWNWFRSEAQVAAECLLLLSIVEHACL
                     RWPLRRDRLYALGLSAGGAMALTLGLRHPERFVAVGSHSGAVPASASNPLQAAAAMQG
                     RRLPDVKQLRLRLFGRRPPPMIVLHGDADRIVAPACAQASAALWLELLPAGTALKSRG
                     RELQRGERLGMHVTDWQHADGDPYVRAVRVHELGHAWSGGAADQAFSEPAGPDALTLA
                     WRFFDAATEARGRR"
     gene            1230..1379
                     /locus_tag="PLQ18_08190"
     CDS             1230..1379
                     /locus_tag="PLQ18_08190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE01669.1"
                     /translation="MYLQGRRLIRYSRGHVTVLDRSGLEAASCPRYEADRRIYARIMD
                     SMTRA"
     gene            complement(1387..2316)
                     /locus_tag="PLQ18_08195"
     CDS             complement(1387..2316)
                     /locus_tag="PLQ18_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HNE01670.1"
                     /translation="MGAAIQPTDLGFFATLAASPSLSAAARELGISTAAVSRHLAQIE
                     ARLGVLLVNRTSRRMGLTPEGEVFLEHARRILGEIDDLDQLLADARGTPKGLLRVNAT
                     LGFGRLHVAPVTSRYVASHPGVDVQLQLSVSPPPVTDDLYDVCVRFGEPADGRVIARR
                     LAGNRRLLCASPRYLAAHGVPQTPDDLRRCNCITIRQGDDAYGTWRLSDARAPATGEE
                     TVKVRGNLTTNDGEIAVHWALDGHGILLRAEWDIARYLQSGRLVQVLPDYRTPDADIY
                     CVYPRRHQLSQRVRTFVDFLAHAFATAGAAQPR"
     gene            2433..>3035
                     /locus_tag="PLQ18_08200"
     CDS             2433..>3035
                     /locus_tag="PLQ18_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010810612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HNE01671.1"
                     /translation="MKTYKIATIPGDGIGKEVVPAGREVLAAVAAAGQGFRFEFTDYD
                     WGGDYYRTHGVMMPEDGLETLRGQDAILFGSAGDPDIPDHITLWGLRLKICQGFDQYA
                     NVRPTRILPGIDAPLKRCRAEDLDWVIVRENSEGEYSGVGGRVHQGHPIEAATDVSMM
                     TRAGVERIMRFAFRLAESRPRKLLTVITKSNAQRHAMVMWD"
BASE COUNT          440 a         1106 c         1077 g          412 t
ORIGIN      
        1 gaggaaggca gctggggtct cgggccgctg gcgcagcggc gcttccggct gtacctgccg
       61 ccgggggcga cacgcgcccg gccggtgccc gtcctgctgc tgctgcacgg ctgcggacag
      121 gacagcgcca gcttcgccgc cagcacgcac gttgcgcgcc tcgcgcagcg cgagcagttc
      181 gcggtgctga tgcccgagca gacgcagcag gccaacgccc agcgctgctg gaactggttt
      241 cgcagcgagg cacaggtcgc cgccgaatgc ctgctgctgc tgtcgatcgt cgaacacgcc
      301 tgcctgcgct ggccgctgcg ccgcgaccgg ctgtacgcgc tcgggctgtc ggccggcggc
      361 gcgatggcgc tcacgctcgg tctgcgtcac ccggaacgct tcgtcgcggt cggctcgcat
      421 tcgggcgcgg tgccggcgag cgccagcaac ccgctgcagg ccgcggccgc gatgcagggc
      481 cggcgcctgc cggacgtgaa acagctgcgc ctgcgtctgt tcggacgccg gccgccgccg
      541 atgatcgtgc tgcacggcga cgccgatcgc atcgtcgcac cggcctgcgc gcaggcctcg
      601 gcggcgctgt ggctggagct gctgccggcc ggcacggcgc tgaaaagccg cggccgcgaa
      661 ctgcagcgcg gcgagcgcct cggcatgcac gtcaccgact ggcagcacgc cgacggcgat
      721 ccctacgtgc gcgccgtgcg cgtccacgaa ctcggccacg cctggagcgg cggcgccgcc
      781 gaccaggcct tctcggaacc ggccgggccg gacgcgctga cgctggcctg gcgtttcttc
      841 gacgcggcga ccgaggcgcg cggacggcgc tgaggcgcgc ccggacagcc gtggaaaccg
      901 gcgccgccag cgcgccggac gacaggcctc gccggcaggc gccacccggc ccggcggcac
      961 catcgcagcc catcgatccc ggacaacggc ctgcacgccc gcggcagacc tgcgtccgcc
     1021 cccgtccggg ccgtcaccgg accgacgccg ccttcccgca gctcccgcca cgttctgacc
     1081 aggccgcccg ccgccggagc ggcccgtccg gccgcgagac cgccagtcat acccatcaca
     1141 gctgaacacg ccgtcttttc agcccctcgc ccctcgcggg agaggggaaa acaacgcatt
     1201 aacgcattta cgcgtgatcg gtatcagtcg tgtacctgca gggccgccgg ctgatccgct
     1261 acagccgcgg ccacgtcacg gtgctcgacc gcagcggtct cgaagccgct tcgtgcccgc
     1321 gctacgaggc cgaccggcgc atatacgcgc ggatcatgga ttccatgacc cgcgcgtaac
     1381 cggggctcag cggggctgcg cggcgccggc tgtggcgaag gcgtgcgcga ggaagtcgac
     1441 gaaggtgcgc acgcgctgcg agagctggtg ccggcgcgga tacacgcagt agatgtcggc
     1501 atccggcgtg cggtagtcgg gcagcacctg caccagccgg ccgctctgca ggtagcgggc
     1561 gatgtcccat tcggcgcgca gcaggatgcc gtgaccgtcg agcgcccagt gcaccgcgat
     1621 ctcgccgtcg ttggtggtca ggttgccgcg caccttcacc gtttcctcgc cggtggccgg
     1681 ggcgcgggcg tcactgaggc gccaggtgcc gtaggcgtcg tcgccctggc ggatcgtgat
     1741 gcagttgcag cggcgcaggt cgtccggggt ctgcggcacg ccgtgcgccg cgaggtagcg
     1801 cggcgaggcg cagagcaggc ggcggttgcc cgccagccgc cgcgcgatca cgcgaccgtc
     1861 ggccggttcg ccgaagcgca cgcagacgtc gtagagatcg tcggtgaccg gcggcggact
     1921 gaccgacagc tgcagctgca cgtcgacgcc ggggtggctc gcgacgtagc gcgaggtcac
     1981 cggggcgacg tgcagccggc cgaagccgag cgtggcgttg acacgcagca gccccttcgg
     2041 cgtgccgcgc gcatcggcga gcagctgatc cagatcgtcg atctcgccga ggatgcgccg
     2101 cgcgtgctcc aggaacacct cgccctcggg cgtgagcccc atgcgccggc tggtgcggtt
     2161 gaccagcagc acgccgaggc gcgcctcgat ctgcgccaga tgccggctga ccgccgccgt
     2221 gctgatgccg agctcgcgcg ccgccgcgct caggctgggg ctggccgcga gcgtggcgaa
     2281 gaaaccgaga tcggtcggct ggattgcagc tcccatgagc gggcccgcct ttaacttcag
     2341 gttaatgacg gtttgagcct agccccgctc cgggagaagg acgagtccgt acagtccgct
     2401 cgtccacacc accccgagag aggagaaccc ccatgaagac ctacaaaatc gccaccatcc
     2461 ccggcgacgg catcggcaag gaggtcgtgc ccgcgggccg cgaggtactc gccgccgtcg
     2521 ccgctgccgg ccagggtttc cgcttcgagt tcaccgatta cgactggggt ggcgactact
     2581 accgtacgca cggcgtgatg atgccggagg acggcctgga gacgctgcgc ggccaggacg
     2641 cgatcctgtt cggctcggcc ggcgacccgg acatccccga ccacatcacg ctctggggcc
     2701 tgcgcctgaa gatctgccag ggcttcgacc agtacgcgaa cgtgcgtccg acccgcatcc
     2761 tgccgggcat cgacgcaccg ctgaagcgct gccgcgccga ggatctggac tgggtgatcg
     2821 tgcgcgagaa ctccgaaggc gagtactcgg gcgtcggcgg gcgcgtgcat cagggccatc
     2881 cgatcgaggc cgccaccgat gtttcgatga tgacccgcgc cggcgtcgag cgcatcatgc
     2941 gcttcgcttt ccgcctcgcc gaatcgcggc cacgcaagct gctgaccgtg atcaccaagt
     3001 ccaacgccca gcgccacgcc atggtgatgt gggac
//
LOCUS       DAOQJH010000333         3031 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_21763_length_3031_cov_2.920699, whole genome shotgun sequence.
ACCESSION   DAOQJH010000333 DAOQJH010000000
VERSION     DAOQJH010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3031
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21763_length_3031_cov_2.920699"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            10..150
                     /locus_tag="PLQ18_08205"
     CDS             10..150
                     /locus_tag="PLQ18_08205"
                     /inference="COORDINATES: protein motif:HMM:NF016432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HNE01672.1"
                     /translation="MLTLYYYEELNLRQIGEVMHVSESRISQIRTRALKRLRELVEEG
                     AR"
     gene            147..776
                     /locus_tag="PLQ18_08210"
     CDS             147..776
                     /locus_tag="PLQ18_08210"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006747142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0106141 - flavin prenyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HNE01673.1"
                     /translation="MSRAPIALALTGASGAQYGLRLLECLLAAGETVYLMYSKAAQIV
                     VGMETDWALPARPAEVRAALLERFGCPPAQLQVYGREEWTAPVASGSAVCRAMAICPC
                     TTGTLAAVAHGLSDTLIERAADVMLKEHRKLVLVVRETPFSAIHLENMLTLARAGAVI
                     LPANPGFYHRPQCVGELVDYIVARVLDQLGVAHALMARWGQPDDPETAS"
     gene            773..1369
                     /locus_tag="PLQ18_08215"
     CDS             773..1369
                     /locus_tag="PLQ18_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018952604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HNE01674.1"
                     /translation="MTLPDEIPLFPLDVVLFPGGPLPLRIFEPRYVSMVGRCLREDLP
                     FGVVPISAGSEAGGDASFHLLGSSARIRDFDRLPDGMLGITCRGERRFRVLEHRSQPD
                     RLVLGRVEWLPEPVREPVPPQFRSLERLLGRILDAEGIERYRALLEPDWDSADWVVNR
                     LAEVMPLPNGARHMLLGIDEPLERFELLYALLQDQRLV"
     gene            1497..3017
                     /locus_tag="PLQ18_08220"
     CDS             1497..3017
                     /locus_tag="PLQ18_08220"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HNE01675.1"
                     /translation="MTPDAVDPARLQFAFTAMAHFLFVPLSLGLAVLLAIMESVFVMT
                     GKAVYREMTQFWARPFAISFALGVATGLPLEYQFGTHWAAFARFGGDVFGLPLALEGL
                     MAFLLESTFIGLFFLGWQRLGRLQHLGVTWLLAIGSNLSAFWILIANAWMQHPVGARL
                     NPETLRMELSSFAELVFNPVAQVKFVHTVAACYVTGASFVLAVSAWYLLRGRVRALAR
                     RSFAIAAAFGLAAVLSVITLGDESGYELGNVQKVKLAAIESMWETGPPPAPFTLFGIP
                     DEQAMRTDHAVRVPWLLGLIATRSIDRPVAGLKELIAGSETRIRRGLIAYEAGERLRA
                     GERDAQLRAQFERHAGDYGYALLLKRYVRDPRQAGEADIARAARDTVPRVWPLFWGFR
                     IMVGCSLLMLGLYGWAFVASARDRLGQRGLLRAALWAWPLPWIASQCGWLISEYGRQP
                     WTVAGLLPTFLAASAHDAAAVTASLGAFIGLYALLFATGIFLIVRCVRRGPEALSA"
BASE COUNT          374 a         1075 c         1075 g          507 t
ORIGIN      
        1 gaccggctcg tgctcacgct ctactactac gaggaactga acctccgcca gatcggcgaa
       61 gtgatgcacg tctccgagtc gcgcatctcg cagatccgca cgcgcgcgct gaagcgcctg
      121 cgcgagctgg tggaggaggg cgcgcggtga gtcgtgcccc gattgcgctg gccctgaccg
      181 gtgcctccgg cgcgcagtac ggcctgcgcc tgctcgaatg cctgctcgcc gccggcgaaa
      241 ccgtctatct gatgtattcg aaggccgcgc agatcgtcgt cggcatggaa accgactggg
      301 cgctgccggc gcgaccggcc gaggtgcgtg cggccttgct cgaacgcttc ggctgcccgc
      361 cggcgcagct gcaggtgtac gggcgcgagg aatggacggc gccggttgca agcggctcgg
      421 ccgtgtgccg ggcgatggcg atctgcccgt gcacgaccgg cacgctggcg gcggtcgcgc
      481 acgggctgtc cgacacgctg atcgaacgcg ccgccgacgt gatgctgaag gagcaccgca
      541 agctggtgct ggtggtgcgc gagacgccgt tctcggcgat ccacctcgaa aacatgctga
      601 cgctcgcgcg tgccggggcc gtgatcctgc cggccaatcc cggtttctat caccggccgc
      661 agtgcgtcgg ggaactcgtc gactacatcg tcgcgcgcgt gctcgaccag ctcggcgtgg
      721 cgcacgcgct gatggcgcgc tggggccagc ccgatgaccc ggagactgcg tcgtgaccct
      781 gccggacgaa atcccgctgt tcccgctcga tgtcgtgctg ttccccggcg gtccgctgcc
      841 gctgcgcatc ttcgagccgc gctacgtcag catggtcgga cgctgcctgc gcgaggacct
      901 gcccttcggc gtcgtgccga tcagcgccgg cagcgaggcc ggcggcgacg cgagcttcca
      961 cctgctcggc agcagcgccc gcatccgtga tttcgaccgc ctgcccgacg gcatgctcgg
     1021 catcacctgc cgcggcgaac ggcgctttcg cgtgctcgaa caccgcagcc agcctgaccg
     1081 gctggtgctc gggcgcgtcg aatggctgcc cgagccggtg cgcgagccgg tgccgccgca
     1141 gttccgctcg ctggagcggc tgctcgggcg catcctcgat gccgagggca tcgaacgcta
     1201 ccgcgcgctg ctcgaacccg actgggacag cgccgactgg gtcgtcaacc gcctcgccga
     1261 ggtcatgccg ctgccgaacg gcgcgcggca catgctgctc ggcatcgacg agccgctgga
     1321 gcgcttcgaa ctgctgtacg cgctgctgca ggatcagcgg ctggtctgaa ggcgctcccg
     1381 gcgcacgccg ggagcggcag gcgcgagacc ggctgcgttg ctgcgttgca gcggaacagc
     1441 ggcgcttgcg ctggtgtcca ccgggtcacg cccgtccccc gcgagacccg ccgccgatga
     1501 cgcctgacgc cgtcgatcct gcacgcctgc agttcgcgtt caccgcgatg gcgcatttcc
     1561 tgttcgtgcc gctgtcgctc gggctcgccg tgctgctcgc gatcatggaa tccgtgttcg
     1621 tgatgaccgg caaggcggtg taccgcgaga tgacgcagtt ctgggcgcgg ccgttcgcga
     1681 tcagcttcgc gctcggcgtc gccaccggcc tgccgctcga ataccagttc ggcacgcact
     1741 gggcggcctt cgcgcgcttc ggcggcgacg tgttcgggct gccgctcgcg ctcgagggcc
     1801 tgatggcctt cctgctggaa tcgaccttca tcggcctgtt cttcctcggc tggcagcggc
     1861 tcgggcggct gcagcacctc ggcgtgacct ggctgctcgc gatcggctcg aacctgtcgg
     1921 cgttctggat cctgatcgcc aacgcctgga tgcagcaccc ggtcggtgcg cggctgaatc
     1981 cggaaaccct gcgcatggag ctttcgagct tcgcggagct ggtgttcaac ccggtcgcgc
     2041 aggtgaagtt cgtgcacacg gtcgccgcct gctacgtgac cggcgcctcg ttcgtgctgg
     2101 cggtcagcgc ttggtatctg ctgcgcggcc gggtgcgggc gctggcgcgg cgcagcttcg
     2161 cgatcgccgc ggccttcggg ctggcggcgg tgctgtcggt gatcacgctc ggcgacgaga
     2221 gcggttacga gctcggcaac gtgcagaagg tcaagctcgc cgcgatcgaa tcgatgtggg
     2281 aaaccgggcc gccgccggcg ccgttcacac tgttcggcat ccccgacgaa caggcgatgc
     2341 gcaccgatca cgccgtgcgc gtgccctggc tgctcgggct gatcgcgacg cgctcgatcg
     2401 accggccggt ggccgggctc aaggagctga tcgccgggag cgagacccgc atccgccgcg
     2461 gcctgatcgc gtatgaggcc ggcgagcgcc tgcgggccgg cgagcgcgat gcgcagctgc
     2521 gcgcgcagtt cgagcgccac gccggcgact acggctacgc gctgctgctg aagcgctacg
     2581 tccgcgatcc gcgtcaggcg ggcgaggccg acatcgcccg cgccgcgcgc gacaccgtgc
     2641 cgcgggtctg gccgctgttc tggggcttcc ggatcatggt cggctgcagc ctgctgatgc
     2701 tcggcctgta cggctgggcc ttcgtcgcca gtgcccgcga ccggctcggg cagcgcggcc
     2761 tgctgcgcgc cgcgctctgg gcgtggccgc tgccgtggat cgcgagccag tgcggctggc
     2821 tgatctccga atacggccgc cagccgtgga cggtcgccgg cctgctgccg accttccttg
     2881 ccgcctcggc ccacgatgcc gcggcggtca cggcgtcgct cggcgccttc atcggcctgt
     2941 acgcgctgct gttcgcgacc ggcatctttc tcatcgtgcg ctgcgtgcgc cgcggcccgg
     3001 aggcgctttc cgcatgaacg gcatcgacta c
//
LOCUS       DAOQJH010000334         3030 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_21770_length_3030_cov_3.957647, whole genome shotgun sequence.
ACCESSION   DAOQJH010000334 DAOQJH010000000
VERSION     DAOQJH010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3030
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21770_length_3030_cov_3.957647"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(143..2413)
                     /locus_tag="PLQ18_08225"
     CDS             complement(143..2413)
                     /locus_tag="PLQ18_08225"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNE01676.1"
                     /translation="MSAQDFHGSTGAAGVPAADDDDLLFLPEGPEAGPERIASPQRWR
                     VLAVDDDRDFQRSTAFALSGMRVLGRRIELLQAYGHAEAARLLAGERDIAVMLLDVVM
                     ETDDAGLRLARAVREVLGNAELRIVLVTGQPGLAPVHEVMERYDINDYWTKSELGVER
                     LHTLLTAGVRAWEQLRTVARARRGLQMIVEASNVLVGARSLRDFSARTIAEIASVLGV
                     PPEGVVCVRRARDDGRVIGAAGRYARSVDRPLEALEPAGVREAIRHCLQERRTLVLAD
                     CMVLYFPAELAGAEYAAWVEIERSLDDTEQELLRVFALNIGSGLHNVALFSRLDDLAY
                     RDQLLGLPNRNALLRALESALGSAARHHTALLLIDIDNFSGVNLTLGPAYGDALLKRI
                     GERLRDGFDPTVLLARLGGDVFGALGPVERLDPESVAALFADGAPSAADGGAWSVGTA
                     RLPLAEFTGTALEALASATLLLKQAKLRGHGQHQDWLPEIAVAAGRRFALLQALREAL
                     ARDEIGIELQPQIDLATGTVTGVEALARWQRADGTRVAPDAFIPLAEATGLIVPLGEH
                     FIELACAAAARLAAAGHGHIRVAVNVSAVQSARADLVASIERQLLASGAEARQMEIEL
                     TETVAMQNFGAVTDTLERLRARGLTVAIDDFGTGFSSLAYLRRLPADLIKIDRSFVQE
                     LGNGGESRTEIAELIIRLAERLGLRVLAEGVETEAQAGWLRMHGCHYAQGWLYARAMP
                     LDALLDWLARREHAGT"
     gene            complement(2556..>3030)
                     /locus_tag="PLQ18_08230"
     CDS             complement(2556..>3030)
                     /locus_tag="PLQ18_08230"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012703207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HNE01677.1"
                     /translation="VALNEVFVPILQKQFPEITDFYLPPEGCSYRLAVVTIRKQYPGH
                     AKRVMLGVWSFLRQFMYTKFVIVTDEDIDARRWEDVIWAMTTRMDPARDTVTIENTPI
                     DYLDFASPVSGLGSKIGFDATHKWPGETTREWGTPIRMDDAIRARVDSLWPELGL"
BASE COUNT          427 a         1082 c         1097 g          424 t
ORIGIN      
        1 gtgccgtccg tagcgcgccg gacgcaccag caggaggcga caccggcgcg gatgctgcac
       61 cgttgcgggg ccagccatcc gcctgcgtgc cggccagcgc cggcagccgc cgcggaagct
      121 tccgccggcg ccgggggaac cttcaggtgc cggcgtgctc gcgccgcgcc agccagtcga
      181 gcagcgcatc gagcggcatc gcccgcgcat acagccagcc ctgcgcgtaa tggcagccgt
      241 gcatgcgcag ccagccggcc tgcgcctcgg tctccacgcc ctcggcgagc acgcgcaggc
      301 ccaggcgctc ggcgagccgg atgatcagtt cggcgatctc ggtgcgcgat tccccgccgt
      361 tgccgagttc ctgcacgaag ctgcggtcga tcttgatcag gtcggccggc agccggcgca
      421 gataggcgag cgacgagaag ccggtgccga agtcgtcgat ggcgacggtc aggccgcgcg
      481 cgcgcaggcg ttcgagcgtg tcggtgacgg cgccgaagtt ctgcatcgcg acggtctcgg
      541 tcagttcgat ctccatctgc cgcgcctcgg cgccgctggc gagcagctgg cgctcgatgc
      601 tcgcgaccag gtcggcgcgc gccgactgca cggccgagac gttgaccgca acgcggatgt
      661 ggccgtgacc ggccgcggcg agccgggccg cggcggcgca ggcaagctcg atgaagtgct
      721 cgccgagcgg cacgatcagc ccggtggcct cggcgagcgg gatgaaggca tcgggtgcga
      781 cgcgcgtgcc gtcggcgcgc tgccagcgcg ccagcgcctc gacgccggtg acggtgccgg
      841 tcgcgaggtc gatctgcggc tgcagctcga tgccgatctc gtcgcgcgcg agcgcctcgc
      901 gcagcgcctg cagcagcgcg aagcgccggc cggcagcgac ggcgatctcc ggcagccagt
      961 cctgatgctg gccgtggccg cgcagcttgg cctgcttcag cagcagcgtg gcgctcgcca
     1021 gcgcctccag cgcggtgccg gtgaactccg caagcggcag gcgcgcggtg ccgacgctcc
     1081 aggcgccgcc gtccgcggcc gacggcgcac cgtcggcgaa cagggcggcg acgctttcgg
     1141 gatcgagccg ttcgaccgga ccgagcgcgc cgaacacgtc gccgccgagg cgcgcgagca
     1201 gcacggtcgg gtcgaagccg tcgcgcaggc gctcgccgat gcgcttgagc agggcgtcgc
     1261 cgtaggccgg gccgagcgtc aggttgacgc cggagaagtt gtcgatgtcg atcagcagca
     1321 gcgcggtgtg gtggcgggcg gcgctgccga gtgcgctttc cagcgcgcgc agcagcgcgt
     1381 tgcggttcgg cagcccgagc agctggtcgc ggtaggcgag atcgtcgagg cggctgaaca
     1441 gcgcgacgtt gtgcaggccg ctgccgatgt tcagcgcgaa cacgcgcagc agttcctgtt
     1501 cggtgtcatc gagcgagcgc tcgatctcga cccaggccgc gtattcggcc ccggccagct
     1561 cggccgggaa gtacagcacc atgcagtcgg cgagcaccag cgtgcgccgc tcctgcaggc
     1621 agtggcggat cgcctcgcgc acgccggccg gctccagggc ttcgagcgga cggtcgacgc
     1681 tgcgcgcgta gcggccggcg gccccgatca cgcggccgtc gtcgcgcgcg cggcgcacgc
     1741 agaccacgcc ttcgggcggc acgccgagca ccgaggcgat ctcggcgatc gtgcgcgccg
     1801 agaagtcgcg caggctgcgc gcgccgacca gcacgttgct ggcctcgacg atcatctgca
     1861 ggccgcgccg ggcgcgcgcc acagtgcgca gctgctccca ggcgcgcaca ccggcggtca
     1921 gcagcgtgtg caggcgttcg acgccgagtt cggacttggt ccagtagtca ttgatgtcgt
     1981 agcgctccat gacctcgtgc accggcgcaa gccccggctg cccggtcacg agcacgatgc
     2041 gcagctccgc gttgccgagc acctcgcgca ccgcgcgcgc cagccgcagg ccggcgtcgt
     2101 cggtttccat gaccacgtcg agcagcatca ccgcgatgtc gcgctcgccg gcgagcaggc
     2161 gcgcggcctc ggcgtggccg taggcctgca gcagctcgat gcgccggccg agcacgcgca
     2221 tgccggacag cgcgaaggcg gtcgagcgct ggaagtcgcg gtcatcgtcg acggcgagca
     2281 cgcgccagcg ctgcgggctc gcaatccgct ccggcccggc ctccggccct tcgggcagga
     2341 acagcagatc gtcgtcatcg gctgccggga cgccggccgc tccggtgctg ccatggaagt
     2401 cttgcgcgct catcgtccgt ctccgctgac cggccgcccg catgcgccgg tcttccctga
     2461 cgtgtgccgg gtcacggacc cggacgaggg cgcagcataa acgtttgccg gccattcatg
     2521 ttgcaccgca ccacaagctt tgcgcctcat gcggctcaca gccccagctc gggccacagg
     2581 ctgtcgacgc gcgcgcggat cgcgtcgtcc atgcggatcg gggtgcccca ctcgcgcgtg
     2641 gtttcgccgg gccacttgtg ggtggcatcg aagccgatct tcgaaccgag tcccgagacc
     2701 ggcgacgcga aatccagata gtcgatcggc gtgttttcga tcgtcaccgt gtcgcgcgcc
     2761 gggtccatgc gcgtggtcat cgcccagatc acgtcctccc agcggcgggc atcgatgtcc
     2821 tcgtcggtga cgatcacgaa cttcgtgtac atgaactggc gcaggaagct ccagacgccg
     2881 agcatcacgc gcttggcgtg gcccgggtac tgcttgcgga tggtcacgac cgccagccgg
     2941 tacgagcagc cctcgggcgg cagatagaag tcggtgatct ccggaaactg cttctgcagg
     3001 atcggcacga acacctcgtt cagcgccacg
//
LOCUS       DAOQJH010000335         3029 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_21786_length_3029_cov_3.362475, whole genome shotgun sequence.
ACCESSION   DAOQJH010000335 DAOQJH010000000
VERSION     DAOQJH010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3029
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21786_length_3029_cov_3.362475"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..221
                     /locus_tag="PLQ18_08235"
     CDS             <1..221
                     /locus_tag="PLQ18_08235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE01678.1"
                     /translation="LAELFADPGLRTIEFYGRAMDWHRRLTDDLRTIYPRGYIRWPQL
                     RRLVHWLRDARHGTASPRETLEHAETER"
     gene            202..882
                     /locus_tag="PLQ18_08240"
     CDS             202..882
                     /locus_tag="PLQ18_08240"
                     /inference="COORDINATES: protein motif:HMM:NF024806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNE01679.1"
                     /translation="MPKPSAELPALLLGMLLLAGPLAGFAQQNCGSLENAFGPYNYND
                     PLIRQSGYGGSKGTIIDLVEGAHLRTFESLSRPDFETMGSIDYTLRAIPNNPRALFLL
                     IRLHRKLNGNLPKPPRVVGDSSAWPGTPECYFERAMRFVPRDPIVRQLLGVYLHWNGK
                     YQQALDAYGQATDLGLKSAELYYNIGLAHFELKQYAEAKDNAHKAYDMGYPSPGLRNK
                     LGSVGQWP"
     gene            1067..2758
                     /locus_tag="PLQ18_08245"
     CDS             1067..2758
                     /locus_tag="PLQ18_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019558804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HNE01680.1"
                     /translation="MLCAEHPGALAHDEAGRCALLVGHPQWRDGALASVARRHGDAAA
                     ALAGWQRFGNGLPERLAGDFSLVIVDAPGGCVFAATDRIGRQPLYHAHCAGGLAFASS
                     AEALARLPGVDASIRPQAVHAYLYFHMVPSPLTIHAGIDKLPGAHRLEWRGADCDIAC
                     WWQPQFREETGADAGRLGREMRALIDNAVADLAAGHRCGVFLSGGLDSSTVAGVLARL
                     KPADADTFSIGFDVPGYDETGFARIAAGRFGTRQHVYYVSADDVLAAVQDISAHYDEP
                     FGNSSAIPAYYCARLAREAGVERLLAGDGGDELFAGNERYLKQKVFEHWLRLPPALRE
                     PLGAGLKALPAIGALGKAASYVRQAETPLPERLETYNFLHRIPAATMFEPDFLQAVDT
                     GWPLGWLRRLWTQAGEASALNRMMYLDWQRTLADNDLRKVERTCALAGVDVVYPLLDD
                     RLIEFSCRVPSALKLPGSRLRDFYKQAMRGFLPEEILNKSKHGFGLPFGIWMRSHAGL
                     RALAGDSLQKLARRGWLRPGFIDEALGLHGSGHAAYYGELVWVLMMLELWLDTHV"
     gene            complement(2813..>3029)
                     /locus_tag="PLQ18_08250"
     CDS             complement(2813..>3029)
                     /locus_tag="PLQ18_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNE01681.1"
                     /translation="YPNGKPGADYGPEHVAMVRELGFDCAVVTSHGVSVAATDPYQLA
                     RFTPWDRSESRFQLRMLRNSRRLHLAA"
BASE COUNT          449 a         1103 c         1029 g          448 t
ORIGIN      
        1 atctggccga gctgttcgcc gatccgggcc tgcgcacgat cgagttctac ggacgggcga
       61 tggactggca ccggcgcctg accgatgacc tgcgcacgat ctacccccgc ggctacatcc
      121 gctggccgca gctgcgccgg ctcgtgcact ggctgcgcga tgcccgccac ggcacggcct
      181 cgccccggga gacactggaa catgccgaaa ccgagcgctg agctgccggc cctgctgctg
      241 ggcatgctgc tgctggccgg gccgctggcc gggttcgcgc agcagaactg cggcagcctg
      301 gaaaatgctt tcgggccata caattacaat gacccgctga tacgccagag cggttacggc
      361 ggctccaaag gcaccatcat cgatctcgtc gaaggcgcac acctgcgtac tttcgaatcc
      421 ctgagccgtc ccgacttcga gacgatgggc agcatcgact acacgctgcg cgcgatcccg
      481 aacaatccgc gcgcgctgtt cctgctgatc cgcctgcacc gcaagctcaa cggcaacctg
      541 ccgaaaccgc cgcgcgtggt cggcgacagc agcgcgtggc cggggacacc ggagtgctac
      601 ttcgagcgtg cgatgcgctt cgtgccgcgc gatccgatcg tgcgccagct gctcggcgtc
      661 tacctgcact ggaacggcaa gtaccagcag gcgctcgatg cctacgggca ggccaccgat
      721 ctcggcctca agagcgccga actctattac aacatcggcc tcgcgcactt cgagctgaag
      781 cagtacgccg aagccaagga caacgcacac aaggcctacg acatgggcta tccgagtccg
      841 ggcctgcgca acaagctcgg ctcggtcggc cagtggccgt gagcgctgcg cgctgacgcc
      901 ggtcaccgga aatctccctg cccccgacga atccgccgat cccatgcatg cattccgtgg
      961 ctggcacagc ctggaccgcc ccgtccccga tgcggtgcaa gccgccctcc ttccgcccgc
     1021 aggcagcgtc gtcgcgcccg gaaccacgcg gagcgcgccc ggcacggtgc tctgcgccga
     1081 gcatcccggc gcactggcac acgatgaggc cggacgctgc gcgctgctcg ttggacatcc
     1141 gcagtggcgt gacggcgctc tggcctccgt ggcccgccgc cacggcgacg cagccgcggc
     1201 actcgccggc tggcaacgct tcggcaacgg gctgcccgaa cggctcgccg gtgatttctc
     1261 gctggtcatc gtcgatgcgc cgggcggctg cgtgttcgcc gccaccgacc gcatcggccg
     1321 ccagccgctc tatcacgcgc attgcgccgg cgggctggcg tttgcatcga gcgccgaggc
     1381 gctggcccga ctgccgggcg tcgatgcctc gatccggccg caggccgtgc atgcgtacct
     1441 gtacttccac atggtgccga gcccgctgac gatccacgcc ggcatcgaca agctgccggg
     1501 ggcgcaccgg ctggagtggc gcggggcgga ctgcgacatc gcctgctggt ggcagccgca
     1561 gtttcgcgag gagaccggcg ccgacgccgg ccgcctcggc cgggagatgc gcgcgctgat
     1621 cgacaacgcc gtcgccgatc tcgccgccgg gcaccgctgc ggggtcttcc tgtccggcgg
     1681 actcgattcc tcgaccgtcg ccggcgtgct cgccaggctc aagcccgccg atgccgacac
     1741 cttctcgatc ggcttcgacg tgccgggcta cgacgagacc ggcttcgcgc gcatcgccgc
     1801 cggacgcttc ggcacacgcc agcacgtgta ctacgtcagc gccgacgacg tgctcgccgc
     1861 cgtgcaggac atctcggcgc actacgacga acccttcggc aactcctcgg cgatcccggc
     1921 ctattactgc gcgcggctcg cccgcgaggc cggcgtcgag cggctgctcg ccggcgatgg
     1981 cggcgatgag ctcttcgccg gcaacgagcg ctacctgaag cagaaggtgt tcgagcactg
     2041 gctgcggctg ccgccggcgc tgcgcgagcc gctcggggcc ggcctgaagg cgctgccggc
     2101 gatcggcgcc ctcggcaagg ccgccagcta cgtgcgccag gccgaaacgc cgctgcccga
     2161 gcggctggaa acctacaact tcctgcaccg catcccggcg gcgacgatgt tcgagccgga
     2221 cttcctgcag gccgtcgaca ccggctggcc gctcggctgg ctgcgcagac tctggacgca
     2281 ggccggcgag gccagcgccc tgaaccggat gatgtatctc gactggcagc gcacgctcgc
     2341 cgacaacgac ctgcgcaagg tcgagcgcac ctgcgcgctc gccggcgtcg acgtggtcta
     2401 tccgctgctc gatgaccggc tgatcgagtt ctcgtgccgg gtgccgtcgg cgctgaagct
     2461 gccgggctcg cggctgcgcg acttctacaa gcaggcgatg cgcggcttcc tgcccgagga
     2521 gatcctgaac aagtccaagc acggcttcgg gctgccgttc ggcatctgga tgcgcagcca
     2581 cgccggcctg cgcgcgctcg ccggcgacag cctgcagaag ctcgcgcgcc gcggctggct
     2641 gcgcccgggc ttcatcgacg aggcgctcgg cctgcacggc agcggccacg ccgcctatta
     2701 cggcgagctg gtgtgggtgc tgatgatgct ggagctgtgg ctggacaccc acgtctgagc
     2761 gcggcatccc gcgcgcgccc ggtgcaggcc gggccgcgcg gggattgcgg cttcaggccg
     2821 ccagatgcag ccggcggctg ttgcgcagca tgcgcagctg gaagcgggac tcggagcggt
     2881 cccagggcgt gaagcgcgcg agctgatagg gatcggtggc ggcaaccgag acgccgtggc
     2941 tggtgaccac ggcgcagtcg aagccgagtt cgcgcaccat cgccacgtgc tcggggccgt
     3001 agtcggcgcc gggcttgccg ttcggatac
//
LOCUS       DAOQJH010000336         3016 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_21942_length_3016_cov_3.240797, whole genome shotgun sequence.
ACCESSION   DAOQJH010000336 DAOQJH010000000
VERSION     DAOQJH010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3016)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3016)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3016
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21942_length_3016_cov_3.240797"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1318)
                     /locus_tag="PLQ18_08255"
     CDS             complement(<1..1318)
                     /locus_tag="PLQ18_08255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNE01682.1"
                     /translation="MTDNGDSPDRPPYRPPAFASTDARSDAGSSAFPWLLFLIVLLPL
                     GLLMLGVAAYVGHARIDAMIAVERANTGIAVGVVSGRLNDELRVPVRHLQALAGELPV
                     RSAITSAEPGAAGLDAAFLSLMERNPNYDQVRWLDAGGRERVRLQREGGQVQRVASVA
                     LQDKSDRPYFREAIRLEAGEMYVSPLDLNIEHGRIEEPYKPMLRVATPVDDANGLRRG
                     LLIINVLAQPILDAAEGSLGSSGNPVTLLNADGYSLRSPEPEQDFGFMFGRSERFATR
                     HAGIWAQMTETPAGQVEDAEGLWTWRRVSLASTAGKTLAGTAPEFIALTQVPGARLVQ
                     QRREVWLAVGGITAALLLAHTLLSLALAFALAGRSRAETGAALARAQAHNAERLQAAQ
                     ARYRSVVEGNINGLLVVGPDGRIDLVNPALARMFGYTVDDLRGRSID"
     gene            complement(1477..1920)
                     /locus_tag="PLQ18_08260"
     CDS             complement(1477..1920)
                     /locus_tag="PLQ18_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015451774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNE01683.1"
                     /translation="MPEVIGIDHLYISVSDPARAEDFYDRAFVTALGFRKNRFALDGE
                     PHVQYYNRHFGYVLRPARSPATHDPYAPGLHHFCLRVDSIEDVVAAAGRLRAAGIEAS
                     PATHHPQYAPDYWACFFSDPDGIRLEITNFRQERRERHAQGAVSD"
     gene            complement(2150..>3016)
                     /locus_tag="PLQ18_08265"
     CDS             complement(2150..>3016)
                     /locus_tag="PLQ18_08265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE01684.1"
                     /translation="ACDAAGTRAALGGFVTPPAALRRQIDLPAAVIGEFAKLFGPTLR
                     CTDFALQDWAAESLTCSRRDREQAESVHPGDAPLLRLPYWGGRLLFGGSETAARHAGY
                     LEGALLAAARLAGELGRLAMTGNAAQAAPAAEREATLERLRACLQAPIADWRAGLLAE
                     YRRQLHVVLSGPHGGAAITQRALLGTVGVLYAAALDALVVSEAQVQGVAIERGRSALT
                     PPLLACFEGCNDALIATALEFNRGSCALSNFPQEHRPSAETLHAIRLDLAAAWREFAL
                     TLNARLLPRSVA"
BASE COUNT          435 a         1080 c         1070 g          431 t
ORIGIN      
        1 cgtcgatgct gcgtccgcgc agatcatcga ccgtgtagcc gaacatgcgc gccagcgccg
       61 ggttgaccag atcgatgcgc ccgtccgggc cgaccaccag cagaccgttg atgttgcctt
      121 cgacgacgct gcgataacgc gcctgcgccg cctgcaggcg ctcggcgttg tgcgcctgcg
      181 cacgcgccag cgccgcgccg gtttcggcgc gcgagcgccc ggccagcgcg aaggcgagcg
      241 ccagcgacag cagcgtatgc gcgagcagca gcgcggcggt gatgccgccg accgccagcc
      301 agacctcgcg acgctgctgc accagccgtg cgcctggcac ctgcgtcagc gcgatgaact
      361 ccggtgccgt gccggccagc gtcttgcccg ccgtgctggc gagactgacc cgccgccagg
      421 tccacagccc ttcggcgtcc tcgacctgtc cggccggcgt ctccgtcatc tgcgcccaga
      481 tgccggcgtg ccgggtcgcg aagcgctcgc tgcgcccgaa catgaagccg aagtcctgtt
      541 ccggctcggg gctgcgcagg ctgtagccgt cggcattcag cagcgtcacc ggattgccgc
      601 tgctgccgag gctgccctcg gcggcatcga ggatcggctg cgccagcacg ttgatgatca
      661 gcaggccgcg gcgcaggcca ttggcatcgt cgaccggcgt cgcgacgcgc agcatcggct
      721 tgtacggctc ctcgatgcgg ccgtgctcga tgttgagatc cagcggcgac acgtacatct
      781 cgccggcctc cagccggatc gcctcgcgga agtacggacg atcactcttg tcctgcagcg
      841 ccacgcttgc gacccgctgc acctgtccgc cctcgcgctg caggcgcacc cgctcgcgtc
      901 cgccggcgtc gagccagcgc acctggtcat agttcgggtt gcgctccatc agcgacagga
      961 aggctgcatc cagtccggcc gcgcccggtt cggcgctggt gatggcactg cgcaccggca
     1021 gctcgccggc cagcgcctgc aggtgtcgca ccggcacgcg cagttcgtca ttcagccgcc
     1081 ccgacacgac cccgacggcg atgccggtat tggcgcgttc gacggcaatc atcgcgtcga
     1141 tgcgggcatg gccgacgtag gccgcgacgc cgagcatcag caggccgagc ggcagcagca
     1201 cgatcaggaa cagcagccac ggaaacgcgc tgctgccggc atcgctccgg gcatccgtcg
     1261 acgcgaaagc cggcgggcga tagggagggc ggtctgggga gtcgccattg tcggtcatgg
     1321 tgcatccctg gatcggcact tgggcgccgg gtgtgggtgt ccgcgggcaa agcgctggcg
     1381 gacgtgtatg acatccttac cggcaggctt gaagtacctg attattgact gattaccaca
     1441 ttccggcaaa tgttgttgct gcgcttgcac actgcgtcaa tcgctgactg cgccctgcgc
     1501 gtgacgctcg cgccgctcct gccggaagtt ggtgatttcc agccggatgc cgtccggatc
     1561 gctgaagaag cacgcccagt agtccggcgc gtattgcgga tggtgggtgg ccgggctggc
     1621 ctcgatgccg gccgcgcgca gccgcccggc cgccgcaacc acgtcctcga tcgaatcgac
     1681 gcgcaggcag aagtgatgca ggcccggcgc gtacggatcg tgcgtcgccg ggctgcgcgc
     1741 cgggcgcagc acgtagccga agtggcggtt gtagtactgc acgtgcggct cgccatcgag
     1801 cgcgaagcgg ttcttgcgaa agccgagcgc ggtcacgaag gcgcggtcgt agaaatcctc
     1861 ggcacgcgcc ggatcgctga cgctgatgta gaggtgatcg atgccgatga cttcgggcat
     1921 ggctggggct ccggcaggga cagccgcagg cgcaccgggg tctgcggccg cggcgggatc
     1981 gtcagggatt caggcggcag ggcgctgcag gctcagccgc cccggacccg gtcgcgcttg
     2041 cagccctcag gctactcggc ggacggatcg ggtttgtaat agacgatgcc gcgcaggccg
     2101 gcgcagccct gtcggcgcgg ccgggccggc ggccggcgcg gccttcgttt caggcgacgc
     2161 tgcgcggcag cagccgggca ttgagcgtca gcgcgaactc ccgccaggcg gcggcgagat
     2221 cgagccggat cgcgtgcagc gtctcggccg aaggccggtg ctcctgcgga aagttcgaga
     2281 gcgcgcagga accgcggttg aattcgagcg cggtcgcgat cagcgcatcg ttgcagccct
     2341 cgaagcacgc gagcagcggc ggtgtcagcg ccgagcgtcc gcgctcgatc gccacgccct
     2401 gcacctgcgc ctcgctgacg accagcgcat cgagcgccgc ggcatacagc acaccgaccg
     2461 tgccgagcag cgcccgctgc gtgatcgccg ccccgccgtg cggcccggac agcacgacgt
     2521 gcagttgccg ccggtattcg gcgagcaggc cggcgcgcca gtcggcgatc ggggcctgca
     2581 gacaggcgcg cagccgctcg agcgtggcct cgcgctccgc tgccggcgcg gcttgcgccg
     2641 cgttcccggt catcgccagc cggccgagtt cgcccgccag ccgggccgcg gcgagcagcg
     2701 ccccttcgag atagcccgcg tgccgggccg cggtttccga acccccgaac agcagccgtc
     2761 cgccccagta cggcaggcgc agcagcggcg catcgcccgg atgcacggac tcggcctgct
     2821 cgcgatcgcg ccggctgcag gtcagcgact ccgccgccca gtcctgcagc gcgaagtcgg
     2881 tgcagcgcag cgtcggcccg aacagcttcg cgaactcgcc gatgaccgca gccggcaggt
     2941 cgatctgccg gcgcagcgcc gccggcggcg tgacgaagcc cccgagcgcg gcgcgcgtgc
     3001 cggcggcatc gcaggc
//
LOCUS       DAOQJH010000337         3006 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_22050_length_3006_cov_3.481193, whole genome shotgun sequence.
ACCESSION   DAOQJH010000337 DAOQJH010000000
VERSION     DAOQJH010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3006)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3006)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3006
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22050_length_3006_cov_3.481193"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..786)
                     /locus_tag="PLQ18_08270"
     CDS             complement(<1..786)
                     /locus_tag="PLQ18_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018227509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HNE01685.1"
                     /translation="MAADESLLAAATPRLRILMLVHGFNSLSQRLHVELAARGHVLSV
                     EYDIHDAITLEAVELFRPDVVLAPYLKRAIPEAVWRHVPCLIVHPGIPGDRGPAALDR
                     AILERRSHWGVTVLQAVAEFDAGPIWAHRSFPMREAPKASLYRREVTTAAVEAVLEAL
                     SRLAAGWQPEAQHEGLPGRTHRPVSLAERTIDWTSDRTADVLRKIHSADGYPGVRGEL
                     AGVTCRLYDAHAEHGLRGAPGALLARRGEALCRATVDGAVHTST"
     gene            complement(786..1784)
                     /gene="hypE"
                     /locus_tag="PLQ18_08275"
     CDS             complement(786..1784)
                     /gene="hypE"
                     /locus_tag="PLQ18_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012703485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HNE01686.1"
                     /translation="MQHGGGGRAMAQLVEELFVAAFANPALSALNDQAVLDAGLSGRL
                     VIATDSHVVSPLFFPGGDIGSLAVHGTVNDVAMAAAEPRWLAASFILEEGFALAELAR
                     IVDSMAQAARAAGVAIVTGDTKVVEAGKGDGVFITTTGVGVARAGFETGGTRARPGDV
                     VLVSGAIGDHGIAIMACREGLDFDVPIESDSAALHELVGALCAVLPPPALRVLRDPTR
                     GGLGATLNEIARASGVGMQLAETAIPVHEPVRAACEFLGLDPLYVANEGKLIAIVDPD
                     HAEAALAALRAHPLGREAARIGVCTDDGQRFVELETGFGGRRVIDWIAGEQLPRIC"
     gene            complement(1906..2265)
                     /locus_tag="PLQ18_08280"
     CDS             complement(1906..2265)
                     /locus_tag="PLQ18_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020165986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE01687.1"
                     /translation="MNLTASALLGVLEEAGTDVLTLAEGLGEDELLNSRITRTEIRRL
                     LISMAQTAASLPPALRTRVPEIDWDGWAALAIALNGSEAEVCDGLWHGVRSQVPATLM
                     WLRVYRQNQPELFAFVP"
     gene            complement(2331..>3006)
                     /gene="hypD"
                     /locus_tag="PLQ18_08285"
     CDS             complement(2331..>3006)
                     /gene="hypD"
                     /locus_tag="PLQ18_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HNE01688.1"
                     /translation="IRQAAAEGLGNFSVFCNHVLTPSAIASILESPEVRDLGSVKLDG
                     FVGPAHVSTVIGSRPYEYFAAEYAKPVVIAGFEPLDVLQAVRMLVRQVNAGQSCVENE
                     FARAVSRDGNRKAQKLVAEVFELRKHFDWRGLGRVPYSALRIKSAWADFDAERRFAPE
                     PRTVPDHKACECAAILRGVKRPQDCRLFGSACTPETPMGSCMVSSEGACAAHWSYGRF
                     RSARPA"
BASE COUNT          431 a         1038 c         1120 g          417 t
ORIGIN      
        1 ggtgctggtg tggaccgcgc cgtcgacggt cgcccggcag agcgcctcgc cgcggcgggc
       61 cagcagcgcg ccgggggcac cgcgcagccc gtgttcggcg tgggcgtcgt acagccggca
      121 ggtgacgccg gccagctcgc cgcgcacgcc ggggtagcca tcggcggagt ggatcttgcg
      181 cagcacgtcg gcggtgcggt cgctcgtcca gtcgatcgtg cgctcggcga gcgacaccgg
      241 ccggtgcgtg cgcccgggaa gtccctcgtg ctgcgcttcg ggctgccagc cggcggcgag
      301 gcgcgacagg gcttcgagca cggcctcgac cgccgcggtc gtgacctcgc gccggtacag
      361 gctcgccttc ggcgcctcgc gcatcgggaa gctgcgatgc gcccagatcg ggccggcatc
      421 gaactcggcg accgcctgca gcacggtcac gccccagtgc gaacggcgtt cgaggatcgc
      481 gcgatcgagc gccgccggcc cgcggtcccc ggggatgccc ggatgcacga tcaggcaggg
      541 cacatggcgc cagaccgcct cagggatcgc gcgcttgaga tagggcgcga gcacgacatc
      601 gggccggaac agttcgacgg cttcgagcgt gatcgcgtcg tggatgtcgt attcgaccga
      661 cagcacgtgg ccgcgggcgg cgagttcgac gtgcaggcgc tggctcagcg aattgaagcc
      721 gtgcacgagc atcaggatgc gcagccgcgg tgtcgccgcc gccagcaggg actcgtcggc
      781 ggccatcagc agatcctcgg cagctgttcg ccggcgatcc agtcgatgac gcgccggccg
      841 ccgaagccgg tttcgagttc gacgaagcgt tggccgtcat cggtgcagac gccgatgcgc
      901 gcggcctcgc gaccgagcgg atgcgcgcgc agcgcggcga gggcggcttc ggcgtgatcc
      961 ggatcgacga tggcgatcag cttgccttcg ttggcgacgt acagcgggtc gaggccgagg
     1021 aactcgcagg cggcgcgcac cggctcatgc accgggatcg cggtctcggc gagctgcatg
     1081 ccgacgccgg aagcgcgcgc gatctcgttc agcgtcgcgc cgaggccgcc gcgggtcgga
     1141 tcgcgcagca cgcgcagcgc cggcggcggc agcaccgcgc agagcgcgcc gaccagttcg
     1201 tgcagcgcgg cgctgtccga ttcgatcggc acgtcgaaat ccaggccttc gcggcaggcc
     1261 atgatcgcga tgccgtgatc gccgatcgcg ccgctcacca gcaccacgtc gccgggccgc
     1321 gcgcgcgtgc cgccggtctc gaagcccgcg cgcgcgacgc cgacgccggt cgtggtgatg
     1381 aacacgccat cgcccttgcc ggcctcgacc accttggtgt cgccggtgac gatggccacg
     1441 ccggcggcac gcgccgcctg cgccatcgaa tcgacgatgc gggcaagctc cgcaagcgca
     1501 aagccttcct cgaggatgaa gctcgccgcc agccagcgcg gctcggccgc cgccatcgcc
     1561 acgtcattga ccgtgccgtg cacggcgagg ctgccgatgt cgccgccggg gaagaacagc
     1621 ggcgagacga cgtggctgtc ggtggcgatc accagccggc cggaaagccc ggcatcgagc
     1681 acggcctgat cgttcagcgc cgacagcgcg gggtttgcga acgcggcgac gaacagctcc
     1741 tcgacgagct gcgccatcgc gcggccgcca ccgccgtgct gcatctcgat gcagccgtgg
     1801 cgcaggtcca gtttgcgggg aaagtctctc ggggggctca acggggcaga cctgtcggga
     1861 tcggggggaa atcggggcag ggaagcgctt cgtgcggccc ggggttcagg gcacgaacgc
     1921 gaacagctcg ggctggttct ggcggtagac gcgcagccac atcagcgtcg ccggcacctg
     1981 cgagcgcacg ccgtgccaga gcccgtcgca gacttcggcc tcgctgccgt tcagcgcgat
     2041 cgccagcgcc gcccagccgt cccagtcgat ctcgggcacg cgcgtgcgca gcgcaggcgg
     2101 caggctcgcg gcggtctgcg ccatgctgat cagcagccgg cgaatctcgg tgcgcgtgat
     2161 gcgcgagttc agcagctcgt cctcgccgag gccttcggcc agcgtcagca cgtcggtgcc
     2221 ggcttcttcg agcacgccga gcagggcgga agcggtcagg ttcatgcggg cgactccaga
     2281 aacaaacggg cgtgcgggtg gatgggcgga tgcggatgcg ggtgcggatt tcaggccggg
     2341 cgcgcgctcc ggaagcgccc gtagctccag tgcgcggcgc aggcgccttc gctggagacc
     2401 atgcacgagc ccatcggcgt ttccggcgtg caggcgctgc cgaacagccg gcagtcctgc
     2461 gggcgcttga cgccgcgcag gatcgccgcg cattcgcagg ccttgtgatc gggcacggtg
     2521 cgcggttcgg gcgcgaagcg gcgttcggcg tcgaagtccg cccaggcgga cttgatgcgc
     2581 agcgcgctgt acggcacgcg gccgagcccg cgccagtcga agtgcttgcg cagctcgaac
     2641 acctcggcga ccagcttctg cgccttgcgg ttgccgtcgc ggctgaccgc gcgcgcgaat
     2701 tcgttctcga cgcagctctg tccggcgttg acctggcgca cgagcatgcg cacggcctgc
     2761 agcacgtcga gcggctcgaa gccggcgatg acgaccggct tcgcgtattc ggcggcgaag
     2821 tattcgtacg ggcggctgcc gatcacggtc gagacgtgcg ccgggccgac gaagccgtcg
     2881 agcttgacgc tgccgagatc gcgcacctcg ggcgattcga ggatgctggc gatcgccgag
     2941 ggcgtcagca cgtggttgca gaacacgctg aagtttccga gcccttcggc cgcggcctgg
     3001 cggatc
//
LOCUS       DAOQJH010000338         3001 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_22110_length_3001_cov_4.758656, whole genome shotgun sequence.
ACCESSION   DAOQJH010000338 DAOQJH010000000
VERSION     DAOQJH010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 3001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3001
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22110_length_3001_cov_4.758656"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..422)
                     /locus_tag="PLQ18_08290"
     CDS             complement(<1..422)
                     /locus_tag="PLQ18_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017675368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HNE01689.1"
                     /translation="MNRKTLSERDICTQCITPALAAAGWDFSTQVREEVSFTAGRIIV
                     RGKLHSRGSRKRADYLLYYKPNLPIAVIEAKDNNHGLGDGMQQGLSYAETLDLPFVFS
                     SNGDGFLFHDRTGNAEPVETELALDQFPSPLELWQRYCT"
     gene            complement(655..1521)
                     /locus_tag="PLQ18_08295"
     CDS             complement(655..1521)
                     /locus_tag="PLQ18_08295"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005664175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNE01690.1"
                     /translation="MDIGFIGLGNMGLAMARNLVGAGHRLRAWNRSPIAPGALPGATL
                     VETPAEAFAAEVVFTMLADDAAIRAVLLDSGVLAGAAPGTVHVVSATISPTLAEELEA
                     LHLCGGLGYLSAPVFGRPDAAATAQLSILAAGDPATLAKVRPLFDVIGRRTFVLGDRP
                     AQANIAKLAGNMMIAMAIESMAEAVALTEAHGIDRATFLDLLLQTLFGCRVYETYGHK
                     IATGDYSAGFKMSLGLKDLRLATEAAGNVDAPLVMLNAVRARMSAAIEAGLGEQDWSG
                     IADYGLRGATTS"
     gene            complement(1787..2335)
                     /locus_tag="PLQ18_08300"
     CDS             complement(1787..2335)
                     /locus_tag="PLQ18_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017666264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNE01691.1"
                     /translation="MPDTPLLPRRLHHNAWTTRDMAATRAFYEDVIGLPLVATWCESD
                     RLFGRVRTYVHCFFGLADGSALAFFQFADAPDAEEFSPPIPETPFHHIALKVDKPLQQ
                     AIEQRIAAAGYGEPQTFVLDHGYCRSLYIRDPNGLLLEFTLDPPGAELIAHERQTDAR
                     ATLERWLAGDHASNNCYRGNTI"
     gene            2530..>3001
                     /gene="scpB"
                     /locus_tag="PLQ18_08305"
     CDS             2530..>3001
                     /gene="scpB"
                     /locus_tag="PLQ18_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018718608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HNE01692.1"
                     /translation="MQLKLILEAALLAAGEPLSPERLESLFTEAERPARAELRAALDA
                     LAQDLEARGIELVEVAGGWRLQVRSALAPWVSRLWEDKPGRYSRATLETLALIAWRQP
                     ITRGEIEAVRGVAVNSGIVRALLDRGWIRVLGHREVPGRPALYGTTREFLDHFGL"
BASE COUNT          447 a          938 c         1113 g          503 t
ORIGIN      
        1 gtgcagtaac gctgccagag ttccagcggc gacgggaact ggtcgagtgc gagctccgtt
       61 tcgactggct cggcgtttcc ggtgcggtca tgaaagagga aaccgtcgcc attgctgctg
      121 aagacgaaag gcagatcgag ggtttcggcg tagctcagcc cctgctgcat gccatcgccc
      181 aggccatggt tgttgtcctt ggcctcgatg actgcgatgg gcagattggg tttgtaatac
      241 agcaggtagt cggcgcgttt cctgctgccc cggctgtgca gcttgccgcg cacgatgatc
      301 cgccctgcgg tgaaggaaac ctcttcgcgt acctgtgtgg aaaaatccca gcccgccgcc
      361 gcgagggccg gggtgatgca ctgggtgcag atgtcgcgtt ccgaaagggt tttcctgttc
      421 atccctgtcg cggtctgtcg tggcctatta ccgtccgctc atcttcgcac agccgcaggg
      481 gcggttcgct gatgccggtc accacggccc cggtcagcga caccgctttc ttgtgccttc
      541 agggcaagct gctgcgggca gctcctgatg atcgccggcc tggcgaatcg gccgcgtgaa
      601 ccggctcgcc ggtgcggcgg cgaggtcagg gatggacttc gccgcgcgga agcctcagct
      661 cgtggttgca ccgcgcagtc cgtaatccgc gattccggac cagtcctgct cgccgagtcc
      721 ggcctcgatt gccgcgctca tgcgtgcccg cacggcgttg agcatcacca gcggcgcatc
      781 gacattgccc gcggcttcgg tggcgaggcg caggtccttg aggccgaggc tcattttgaa
      841 gccggccgag tagtcgccgg tggcgatctt gtggccgtag gtctcgtaga cccggcagcc
      901 gaacagcgtc tgcagcagca ggtcgaggaa ggtcgcgcgg tcgatgccgt gggcttcggt
      961 cagcgccacg gcttcggcca tcgactcgat cgccatcgcg atcatcatgt tgccggcgag
     1021 cttggcgatg ttggcctgcg ccgggcgatc gccgagcacg aaggtgcgcc ggccgatcac
     1081 gtcgaacagc ggccgcacct tcgcgagcgt ggccgggtcg ccggcggcga ggatgctgag
     1141 ctgcgcggtc gccgcggcat cggggcggcc gaacaccggg gcggacagat agccgaggcc
     1201 gccgcagaga tgcagggctt cgagttcctc ggcgagcgtc ggcgagatgg tcgccgatac
     1261 cacatgcacg gtgccgggcg cggcgccggc gagcacgccg gaatcgagca gcacggcgcg
     1321 gatcgcggca tcgtcggcga gcatcgtgaa cacgacctcg gcggcgaagg cttcggccgg
     1381 ggtttcgacc agcgtcgcgc cgggcagggc accgggcgcg atcggcgagc ggttccaggc
     1441 gcgcaggcgg tggccggcgc cgacgaggtt gcgggccatg gcgaggccca tgttgccgag
     1501 tccgatgaaa ccgatgtcca tgaatgcctc cgtacgcagg gcggaatgtc aggtcagggc
     1561 gggagggccg ttgcaggcgg ctcagggcgg atgcgtccct gccagcgttg cgccggtcct
     1621 gctcccccgg gggcagcgcg gcggaaaggg cggccggatc atgccggtca gacgtaagtg
     1681 catggttttt acaatcattt ccccaaacgc tctcccgcaa ggggcgagga gctgaacagc
     1741 cgagtgctcc ggtgtaaccg gtatcacatg ctttgcatgc gcaggatcag atggtgttgc
     1801 cgcgatagca gttgttcgaa gcatggtcgc cggccagcca gcgctccagc gtggcacggg
     1861 catcggtctg gcgttcgtgg gcgatcagct cggccccggg cgggtcgagc gtgaattcga
     1921 gcagcaggcc gttcggatcg cggatgtaca gcgagcggca gtagccgtga tcgagcacga
     1981 aggtctgcgg ttcgccgtag ccggcggccg cgatgcgctg ctcgatcgcc tgctgcagcg
     2041 gcttgtcgac cttcagcgcg atgtgatgga acggcgtttc cgggatcggc gggctgaatt
     2101 cctcggcatc gggcgcatcg gcgaactgga agaacgccag cgcgctgccg tcggcaagtc
     2161 cgaagaagca gtgcacatag gtgcggaccc tgccgaacag ccggtcggat tcgcaccagg
     2221 tcgcgaccag cggcaggccg atcacatcct cgtagaaggc acgggtggcg gccatgtcgc
     2281 gcgtggtcca ggcattgtga tgcaggcgac ggggcagcag gggggtgtcc ggcatcgcgg
     2341 gcgactcctc gggtggcggg cgggcggacg cgatcagggc ggcgtccggg cggatacggc
     2401 tacgggaata gcaaacgcgc ggcgccggtg ccgggtttcg tgatgcaccg catcagccgt
     2461 gcgtgcgggg cgggacgcgc gtatcctgcg ccgccggctt tgcgcgcgcg cccggagccc
     2521 gttcgtccga tgcaactgaa gctgatcctc gaagccgcac tgctcgcggc cggcgagccg
     2581 ctgtcgccgg aacggctgga gagcctgttc accgaagccg agcgtccggc gcgcgccgaa
     2641 ctgcgggcgg ccctcgatgc gctggcgcag gatctcgaag cgcgcggcat cgagctggtc
     2701 gaggtcgccg gtggctggcg gctgcaggtg cgttcggcgc tggcgccgtg ggtgtcgcgc
     2761 ctgtgggagg acaagcccgg acgttattcg cgcgcgacgc tggagacgct ggcgctgatc
     2821 gcctggcgcc agccgatcac gcgcggcgag atcgaggccg tgcgcggcgt cgccgtcaac
     2881 agcggcatcg tgcgtgcgct gctcgatcgc ggctggatcc gcgtgctcgg tcaccgcgag
     2941 gtccccggcc ggccggcgct gtatggcacg acgcgcgaat ttctcgatca tttcggtctg
     3001 a
//
LOCUS       DAOQJH010000339         2998 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_22159_length_2998_cov_4.305471, whole genome shotgun sequence.
ACCESSION   DAOQJH010000339 DAOQJH010000000
VERSION     DAOQJH010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2998
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22159_length_2998_cov_4.305471"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..738)
                     /locus_tag="PLQ18_08310"
     CDS             complement(<1..738)
                     /locus_tag="PLQ18_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011629603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF294 nucleotidyltransferase-like
                     domain-containing protein"
                     /protein_id="HNE01693.1"
                     /translation="MPASPDSVAAAAAGTELLDLAALGAALAAAGPPPVRAFREALRA
                     ADTRLRERFRQGVHARVLVPLRARLVDRLLALLWRHFIGEARTDLALLAVGGYGRGEL
                     HPGSDVDIMLLLAAPEDAGTGAAIEQLLTFLWDIGIEVGQSVRTVEDCVRECTSDITV
                     ATNLMEARLITGPEALYADMRTRIAPGRVWPPAAFFEAKRAEQQQRYRKFHDTEYNLE
                     PNVKEGPGGLRDLQMIGWVAKRHFGART"
     gene            complement(756..1523)
                     /gene="map"
                     /locus_tag="PLQ18_08315"
     CDS             complement(756..1523)
                     /gene="map"
                     /locus_tag="PLQ18_08315"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002809397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HNE01694.1"
                     /translation="MAISIKTPEEIEQMRVAGRLAAELLAMIEPHVREGVTTEELDRL
                     CHEYTVNVQQAIPAPLNYRGFPKSICTSVNHVVCHGIPGPKKLKRGDIVNVDVTVIKD
                     GWHGDTSRMFEIGEVPVIAKRVCRISKECMLIGIEMVRPGVRLGDIGHAIQRHAEANG
                     CSIVQEYCGHGIGRVFHEDPQVLHYGKPGSGPELVPGMTFTIEPMVNAGKRHVKLLPD
                     GWTVVTKDHSLSAQWEHTVAVTEHGVDVLTVRPGDPL"
     gene            1804..2550
                     /gene="rpsB"
                     /locus_tag="PLQ18_08320"
     CDS             1804..2550
                     /gene="rpsB"
                     /locus_tag="PLQ18_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013835515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HNE01695.1"
                     /translation="MSTVSMRDMLEAGVHFGHQTRYWNPKMGAYIFGARSKIHIVNLE
                     KTLPLFNDAMNFVGKLAGNGGRILFVGTKRSARTAVAEAAARCGMPYVNHRWLGGMLT
                     NFKTVRQSIKRLKDLEQMSADGSFERLSKKEVILLRREMEKLERSLGGIKDMPALPDA
                     LFVIDVGHEKIAIQEAGKLGIPVVGIVDTNNSPDGVDYVIPGNDDAIRAITLYADGAA
                     AAVMTARGALPVAARADEDFVEVPAERTAG"
     gene            2663..>2998
                     /gene="tsf"
                     /locus_tag="PLQ18_08325"
     CDS             2663..>2998
                     /gene="tsf"
                     /locus_tag="PLQ18_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017364515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HNE01696.1"
                     /translation="MSISAAMVKELRERTGAGMMECKKALTEAGGDIEAAVELMRKQG
                     LAKADKKAGRTAAEGTLVVAAGDKAVALVEVNCETDFVAKGDEFQGFAAAVAASALTA
                     SPADLDALLA"
BASE COUNT          484 a         1004 c         1051 g          459 t
ORIGIN      
        1 ggtccgggca ccgaaatggc gcttggcgac ccagccgatc atctgcaggt cgcgcaggcc
       61 cccggggcct tccttgacgt tcggttcgag gttgtactcg gtgtcgtgga acttgcggta
      121 gcgctgctgc tgctcggcgc gcttggcctc gaagaacgcc gccggcggcc agacgcggcc
      181 cggggcgatg cgcgtgcgca tgtcggcgta cagcgcctcg gggccggtga tcagccgcgc
      241 ttccatcagg ttggtcgcga ccgtgatgtc ggacgtgcat tcgcgcacgc agtcctcgac
      301 cgtgcgcacg ctctggccga cctcgatgcc gatgtcccac aggaaggtca gcagctgctc
      361 gatcgcggcg ccggtgccgg catcctcggg cgcggcgagc agcagcatga tgtcgacatc
      421 ggagcccgga tgcagctcgc cacggccata gccgccgacg gcgagcagcg cgagatcggt
      481 gcgcgcctcg ccgatgaaat ggcgccacag cagcgccagc agccggtcga cgaggcgcgc
      541 gcgcagcggc accagcacgc gtgcgtgcac gccctggcgg aagcgttcgc gcaaccgggt
      601 gtcggcggcg cgcagcgcct cgcggaaggc gcgcaccggc ggcggcccgg cggcggcgag
      661 ggctgcgcca agtgctgcca gatcgagcag ttcggtgcca gccgccgcgg cggcaacgga
      721 atcggggctg gcgggcatgg cgcctccgga agcgttcaga gcggatcgcc ggggcgcacg
      781 gtcagcacgt cgacgccgtg ctcggtcacg gccaccgtgt gttcccactg cgccgacagg
      841 ctgtgatcct tggtgaccac ggtccagccg tcgggcagca gcttcacgtg gcgcttgccg
      901 gcgttgacca tcggttcgat cgtgaaggtc atgccgggca cgagttcggg gccgctgccg
      961 ggcttgccgt agtgcagcac ctgcgggtcc tcatggaaca cgcggccgat gccgtggccg
     1021 cagtattcct gcacgatcga acagccgttg gcctcggcgt ggcgctggat cgcatggccg
     1081 atgtcaccga ggcgcacgcc cgggcgcacc atctcgatgc cgatcagcat gcattccttg
     1141 gaaatgcggc agacgcgctt cgcgatcacc ggcacctcgc cgatctcgaa catgcggctg
     1201 gtatcgccgt gccagccgtc cttgatgacg gtgacatcga cattgacgat gtcgccgcgc
     1261 ttgagtttct tcgggcccgg gatgccgtgg cagacgacgt gattgacgga tgtgcagatc
     1321 gacttcggaa agccgcggta attcagcggc gccgggatcg cctgctgcac attgaccgtg
     1381 tactcgtggc agaggcggtc gagctcctcg gtcgtgacgc cctcgcgcac gtgcggttcg
     1441 atcatcgcca gcagttcggc cgccagacgg ccggcgacgc gcatctgttc gatctcttcg
     1501 ggcgtcttga tggaaatggc catgcaggtc tcgacggcgg gagggcgggg cggcggcagc
     1561 ggcgcgaggc ggtgcgggcc ggcggacggc gaccgggcgc tgttgggtga aaagaccgcg
     1621 tatggtataa agccgccgcc ccgcgggcaa ttgcatgcgg ggccaacctt ccgaagtaca
     1681 acccgagact actcacacgt gaccggcacg tccggcgggg tgcccttttc gtcgtccgga
     1741 cgaacgggtc gccgggcggg gcgcgtggag gctcaacccc gaacttcgtt ccaaggagtc
     1801 ccgatgtcca ccgtttcgat gcgcgacatg ctggaggccg gcgtccactt cggccaccag
     1861 acccgttact ggaacccgaa gatgggtgcc tacatcttcg gcgcgcgcag caagatccac
     1921 atcgtcaacc tcgaaaagac gctgccgctg ttcaacgacg cgatgaactt cgtcggcaag
     1981 ctcgccggca acggcggccg catcctgttc gtcggcacca agcgctcggc gcgcacggcc
     2041 gtggccgaag ccgcggcgcg ctgcggcatg ccgtacgtca accaccgctg gctcggcggc
     2101 atgctgacca acttcaagac cgtgcgccag tcgatcaagc gcctgaagga tctcgagcag
     2161 atgagcgccg acggctcctt cgagcgcctg tcgaagaagg aagtcatcct gctgcgccgc
     2221 gagatggaaa agctcgagcg cagcctcggc ggcatcaagg acatgccggc gctgccggat
     2281 gcgctgttcg tcatcgacgt cggtcacgag aagatcgcga tccaggaagc cggcaagctc
     2341 ggcattccgg tcgtcggcat cgtcgacacc aacaactcgc ccgatggcgt cgattacgtg
     2401 atccccggca acgatgacgc gatccgcgcg atcacgctgt acgccgacgg cgccgccgcc
     2461 gccgtgatga ccgcccgcgg cgcgctgccg gttgccgccc gcgccgacga ggacttcgtc
     2521 gaggtcccgg ccgagcggac cgccggctga tccgtcccgt tccggccgca ggcccgcgag
     2581 cccaaggcaa gcgggccttt ttgcgcgtgc cgtccgggca tgcgctgcat ctttcagatg
     2641 aagcaaagag gaatccactg acatgtccat ttccgcagcc atggtcaagg aactgcgcga
     2701 gcgtacgggc gcgggcatga tggaatgcaa gaaggcgctg accgaggccg gcggcgacat
     2761 cgaggcggcg gtcgagctga tgcgcaagca ggggctcgcc aaggccgaca agaaggccgg
     2821 gcgcaccgct gccgagggca cgctggtggt cgccgccggt gacaaggccg tcgcgctggt
     2881 cgaggtcaac tgcgagaccg acttcgtcgc caagggcgac gagttccagg gcttcgccgc
     2941 tgcggtcgcc gcgagcgcgc tcaccgccag cccggccgat ctcgacgcac tgctggcc
//
LOCUS       DAOQJH010000340         2996 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_22183_length_2996_cov_3.956817, whole genome shotgun sequence.
ACCESSION   DAOQJH010000340 DAOQJH010000000
VERSION     DAOQJH010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2996)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2996)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2996
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22183_length_2996_cov_3.956817"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(119..925)
                     /gene="cysE"
                     /locus_tag="PLQ18_08330"
     CDS             complement(119..925)
                     /gene="cysE"
                     /locus_tag="PLQ18_08330"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013662130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HNE01697.1"
                     /translation="MAVQPLRDSAPATNDPLWETIRDEARITARQEPILASFLHASLL
                     NHRDLGAALSFTLAGKLDGATVPAMVVREVIEAAIEADPVLLDRVRADLLAVRERDPA
                     CPGYSVPLLYFKGFQAIQAYRVAHALWRDGREALALFLQSRIAEVFAVDIHPAARIGQ
                     GILLDHATGLVVGETAIIEDNVSMLQGVTLGGTGKESGDRHPKVRAGVLIGAGAKILG
                     NIEIGTGAKVGAGSVVLDPVPAHCTVVGVPARVVCQCREQEPSRSMDHHI"
     gene            1003..1416
                     /locus_tag="PLQ18_08335"
     CDS             1003..1416
                     /locus_tag="PLQ18_08335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE01698.1"
                     /translation="MILVLLVVAIGLGLWWFFGQHNAASLDNLQASGFHADYTLDHNV
                     SPVALDSRTRRIAFLGLTRVRVYDYRQVISWRFDDYENPGGNDRSEPQVHRELVFRLD
                     DAEHPVVRLAGFDAEAARRWLATLQRELPQAGRGR"
     gene            1582..1920
                     /locus_tag="PLQ18_08340"
     CDS             1582..1920
                     /locus_tag="PLQ18_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006478303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HNE01699.1"
                     /translation="MMSPKFLTAAAFAALLAATAARADELPLYTLTAKDGRFEPATIE
                     VTAGQKFRVEVVNAGSSAIEFESKPLRKEKVIAAGARATIDFQPLKAGSYRFFDEFHE
                     KTGQGEIVAR"
     gene            1954..2802
                     /locus_tag="PLQ18_08345"
     CDS             1954..2802
                     /locus_tag="PLQ18_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013587826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HNE01700.1"
                     /translation="MGDVLFVVWRESVEALLVIGILYAWLRRQPEAGQGLRYLWLGVG
                     LGLGAAVLLGTMVLKAASLLDGDSGEIFQIAMVLFASALIVQMVCWMRRHGRTLKAEL
                     EQGMQRNLASHNWWGMVAVIVIAIAREGSETVIFLYGLGVQQQGGGLLSFVFSALVGL
                     GLAFATFALLQAGTRALSWRTFFRFSEAMLLLLAAALLTTGVEKLVSLGWLPGLVDPL
                     WDSSWLLDDSTTTGGLVAALTGYRAHPALTMLIAYVAYWGVVSWLLRAPALSGKAPSQ
                     VAAARG"
     gene            complement(2891..>2996)
                     /locus_tag="PLQ18_08350"
     CDS             complement(2891..>2996)
                     /locus_tag="PLQ18_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HNE01701.1"
                     /translation="DDMTRVIPLAEAIPAAAELIAGQVRGRLVVDVNA"
BASE COUNT          424 a         1028 c         1054 g          490 t
ORIGIN      
        1 atgcgctgct tttcctgaag cagcttccct gatgcggcga aggccgtccg gtcgcacagg
       61 accggacggc cttcgtgatt tttccgggca gcggccggcg gcggggccgg ggcggccctc
      121 agatgtggtg atccatcgag cgcgacggct cctgctcgcg gcactggcag accacgcgcg
      181 ccggcacgcc gacgacggtg cagtgcgccg gcaccgggtc cagcacgacg ctgccggcgc
      241 cgaccttggc gccggtgccg atctcgatgt tgccgagaat cttggcgccg gcgccgatca
      301 gcacgccggc gcgcaccttc ggatggcggt cgccgctttc cttgccggtg ccgccgagcg
      361 tcacgccctg cagcatcgag acgttgtcct cgatgatggc ggtctcgccg accacgaggc
      421 cggtggcgtg atcgagcagg atgccctgac cgatgcgtgc cgccggatgg atgtcgaccg
      481 cgaacacctc ggcgatgcgg ctttgcagga acagcgccag tgcctcgcgg ccgtcgcgcc
      541 acagcgcgtg cgcgacgcgg taggcctgga tggcctggaa acccttgaag tacaagagcg
      601 gcaccgaata gcccgggcag gccgggtcac gctcgcgcac cgcgagcagg tcggcgcgca
      661 cgcgatcgag cagcaccgga tcggcctcga tcgcagcctc gatcacctcg cgcacgacca
      721 tcgccggcac cgtcgcaccg tcgagcttgc cggccagcgt gaatgacagc gccgcgccga
      781 gatcgcggtg attcagcagc gaggcatgca ggaagctcgc gaggatcggt tcctgacggg
      841 cggtgatgcg tgcctcgtca cggatggttt cccagagcgg gtcgttcgtg gcgggcgcgg
      901 agtcgcgcag gggctggacg gccatcggaa tacctcgccg gttcggaagg gccgccgtgt
      961 cagaatcggg cttcccgatc ctgagccgcg gaggcaactg gaatgatcct ggtcctgctc
     1021 gtcgtggcca tcggcctcgg cctctggtgg ttcttcggcc agcacaacgc ggcgtccctg
     1081 gacaatctgc aggcgagcgg ctttcacgcc gattatacgc tcgaccacaa cgtcagcccg
     1141 gtggccctcg acagccgcac acgccgcatc gccttcctcg gcctgacgcg cgtgcgcgtc
     1201 tacgactacc gccaggtcat cagctggcgt ttcgacgatt acgagaaccc cggcggcaat
     1261 gatcgttccg agccgcaggt ccaccgcgaa ctggtgttcc ggctcgacga tgccgagcac
     1321 ccggtggtcc ggctcgccgg cttcgacgcc gaggcggcgc gtcgctggct ggcgacgctg
     1381 cagcgcgaac tgccgcaggc cgggcgcggg cgctgaggag ctgacgcgaa tcccgcagaa
     1441 aattccgggt tgcgccaaat gagaattgtt tttatctgga cgggtcggca tcatcgctgc
     1501 aagcgtccgc ggacgcaggg ttccgttctg cttcccgtac cgattcctgc caccgatccc
     1561 gtctgccccc cggagtctgc cgtgatgagc ccgaagttcc tgaccgccgc tgccttcgcc
     1621 gcgctgctcg ccgctaccgc cgcgcgcgcc gatgaactgc cgctgtacac gctgaccgcg
     1681 aaggacggtc gcttcgagcc ggcgacgatc gaggtcacgg ccggccagaa attccgcgtc
     1741 gaggtcgtca atgccggcag ctcggcgatc gagttcgaga gcaagccgct gcgcaaggag
     1801 aaggtcatcg ccgccggcgc gcgcgcgacg atcgacttcc agccgctgaa ggccggcagc
     1861 tacaggttct tcgacgagtt ccacgagaag accggccagg gcgagatcgt cgcgcgctga
     1921 gcgcggccgg gagcagtcat catgatgggc atcatgggcg acgtgttgtt cgtggtctgg
     1981 cgcgagagcg tcgaggcgct gctggtcatc ggcatcctgt atgcgtggct gcgccgccag
     2041 cccgaggccg gtcagggcct gcgctatctg tggctcggcg tcggactcgg gctcggggcg
     2101 gccgtgctgc tcggcacgat ggtgctgaag gccgcgagcc tgctcgatgg cgacagcggc
     2161 gagatcttcc agatcgcgat ggtgctgttc gcatccgcgc tgatcgtgca gatggtctgc
     2221 tggatgcgcc gccacggccg cacgctgaag gccgaactcg aacagggcat gcagcgcaac
     2281 ctcgccagcc acaactggtg gggcatggtc gccgtcatcg tcatcgccat cgcccgcgag
     2341 ggcagcgaga cggtgatctt cctgtacggc ctcggcgtgc agcagcaggg cggcgggctg
     2401 ctgagcttcg tgttctcggc gctggtcggc ctcgggctgg cgttcgcgac cttcgcactg
     2461 ctgcaggccg gcacgcgcgc gctgtcgtgg cgcacgttct tccgcttcag cgaggcgatg
     2521 ctgctgctgc tcgccgcggc gctgctgacc accggcgtcg agaagctcgt ctcgctcggc
     2581 tggctgccgg gtctcgtcga tccgctgtgg gacagcagct ggctgctcga tgactcgacc
     2641 acgaccggcg gtctggtcgc ggcgctgacc ggctaccgtg cgcatccggc gctgacgatg
     2701 ctgatcgcct acgtcgcgta ctggggcgtc gtcagctggc tgctgcgcgc cccggcgctc
     2761 agcggcaagg caccgtcgca ggtcgccgcc gcacgcggct gaatccggca ccggctccgg
     2821 cccgcgcccc gctgcgcttg ctgctgcagc ggggcgcggc gtttccggcg ccggctccgg
     2881 catggccgga tcaggcattg acgtcgacga ccagccggcc gcgcacctgc ccggcgatca
     2941 gctcggccgc ggccgggatc gcctcggcga gcgggatcac gcgggtcatg tcatcg
//
LOCUS       DAOQJH010000341         2992 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_22233_length_2992_cov_3.932925, whole genome shotgun sequence.
ACCESSION   DAOQJH010000341 DAOQJH010000000
VERSION     DAOQJH010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2992)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2992)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2992
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22233_length_2992_cov_3.932925"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..661
                     /locus_tag="PLQ18_08355"
     CDS             <1..661
                     /locus_tag="PLQ18_08355"
                     /inference="COORDINATES: protein motif:HMM:NF013197.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE01702.1"
                     /translation="TLVNDWHGRIERVEAGTLQALRAHAVVSAARLSEHSRSAGASTA
                     ANVAAWVGPGRTVLTHGWSTTVLACLAQIAALGGLRVICTEARPLNEGRQLAARAAGL
                     GVPVTLITDAQLALAATAADVVLVGADTVLVDGAVVGKAGTWPMALAARAVDIPFVVA
                     AESCRRSPELSVSLEDQDPAEVGAPLPGVMLRNRRFDVTPPDLVTAWIDEHGVWTGEA
                     A"
     gene            754..1392
                     /locus_tag="PLQ18_08360"
     CDS             754..1392
                     /locus_tag="PLQ18_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017423007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HNE01703.1"
                     /translation="MTGIHDFPAFLLASVALAVLPGPDTLYVLGRTVAQGRRAGLWSL
                     AGIGGGCLGHVAASALGLSALIAASPFAFELVKLAGALYLAWIGVQLLREAWRHAGDG
                     VPLPAVAADEAGGWQLLRQGVITNLLNPKVGLFFISFLPQFVTPGVGTVGEIVLLGLT
                     FVGIGLVWILLLMHTTDLATRHFRGRAAVQRWFKASTGGLFVGLAAKLALDR"
     gene            1415..2245
                     /locus_tag="PLQ18_08365"
     CDS             1415..2245
                     /locus_tag="PLQ18_08365"
                     /inference="COORDINATES: protein motif:HMM:NF013431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HNE01704.1"
                     /translation="MERNHARASDGAVLAACRHGGHLLSWQTPDGRERLFLSPLSQPA
                     PGVAIRGGVPVCFPQFSGFGPLPKHGFVRDRIWQCTAAGTAADGRARLTYALEADAAT
                     RALWPQAFRCELELLAAGDTLAVTLAVHNTGEAPLSFTGALHTYFAVTDLDAARVSGL
                     EAHPYRDHAGAAAAGGGIDHSPDGAPLCFGAEVDRVYAGVRGAIRLATGAGTLQIEAD
                     GFPDAVIWNPGPEKGAALADLEAGAWRRYVCVEAAIVRTPVSVAAGALWRGTQRVRVI
                     "
     gene            2242..>2992
                     /locus_tag="PLQ18_08370"
     CDS             2242..>2992
                     /locus_tag="PLQ18_08370"
                     /inference="COORDINATES: protein motif:HMM:NF013703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HNE01705.1"
                     /translation="MTDAATAVPSRAAILRFAAPVMLANLTTPLLGIVDTAIMGRSPD
                     PALIAAVAVGSTVLTVLFWAFSFLRQSTVGLTAQALGAGEATELAAALLRPLALAALI
                     GTVLILLQAPIAALAFGLMTSAPEVETAARAYYGWRIAAAPFALANYALAAWLIGQRR
                     ARLVLGLQLAMNGGNAALTALFVLGFGLGPAGAGLGTALAEMLTCLATLGLPAVRAQF
                     APAVLRAVCSGGRAQFARLMRARAAGVASVDG"
BASE COUNT          321 a         1098 c         1114 g          459 t
ORIGIN      
        1 gacgctggtc aacgactggc atggacgcat cgagcgcgtc gaggccggta cgctgcaggc
       61 gctgcgcgcg catgccgtgg tcagtgccgc gcgcctgagc gagcactcgc gcagcgccgg
      121 cgcgagcacc gctgccaacg tcgccgcctg ggtcggaccg gggcgcaccg tgctgacgca
      181 cggctggagc acgacggtgc tcgcctgcct cgcgcagatc gccgcgctcg gcggcctgcg
      241 cgtgatctgc accgaggcgc gcccgctgaa cgagggccgc cagctcgcgg cccgcgccgc
      301 cggtctcggc gtgccggtga cgctgatcac cgatgcgcag ctggcgctgg cggcgaccgc
      361 ggccgacgtg gtgctggtcg gcgccgacac cgtgctcgtc gacggtgccg tggtcggcaa
      421 ggccggcacc tggccgatgg cgctggcggc gcgcgccgtc gacattccgt tcgtggtcgc
      481 cgccgagtcc tgcaggcgca gtccggaact gagcgtgtcg ctcgaagacc aggacccggc
      541 cgaggtcggg gcgccgctgc cgggcgtgat gctgcgcaac cgccgcttcg acgtgacgcc
      601 gccggatctg gtcacggcct ggatcgacga gcacggcgtc tggaccggcg aggcggcgtg
      661 aagcgcgctt gccagcggct cacgggccgg ctcgcggggc tgcgggggcg tggctagact
      721 gccgctccct tttcgtgacc ggagccgccc gcaatgaccg gcatccatga tttccctgcc
      781 ttcctgctcg cctcggtggc gctggccgtg ctgccggggc cggacacgct gtacgtgctc
      841 gggcgcaccg tcgcgcaggg ccggcgcgcc gggctctggt cgctggccgg catcggcggc
      901 ggctgcctcg gtcatgtcgc cgcctcggcg ctcgggctgt cggcgctgat cgccgcgtcg
      961 ccgttcgcgt tcgagctggt caagctcgcc ggtgcgctgt acctggcctg gatcggcgtg
     1021 cagctgctgc gcgaggcctg gcggcacgcc ggcgacggtg tgccgctgcc ggccgtggcc
     1081 gcggacgagg ccggtggctg gcagctgctg cgtcagggcg tgatcaccaa cctgctgaat
     1141 ccgaaggtcg ggctgttctt catctccttc ctgccgcagt tcgtgacgcc cggagtcggc
     1201 acggtcggcg agatcgtgct gctcggcctg accttcgtcg gcatcggtct ggtgtggatc
     1261 ctgttgctga tgcacacgac cgatctcgcg acccggcact tccgcggccg ggccgccgtg
     1321 cagcgctggt tcaaggccag caccggcggc ctgttcgtcg ggctggcggc gaagctcgcg
     1381 ctggatcgct gaactgctgc cggagtcacc gtccatggaa cgcaaccacg cccgcgccag
     1441 cgatggcgcc gtgctcgccg cctgccgcca cggcggccat ctgctgtcgt ggcagacccc
     1501 ggacggccgc gagcgcctgt tcctgagtcc gctcagccag ccggcgcccg gcgtcgcgat
     1561 ccgcggcggc gtgccggtgt gcttcccgca gttctcgggc ttcgggccgc tgccgaagca
     1621 cggcttcgtg cgcgatcgga tctggcagtg cacggcggcc ggcaccgctg ctgacggccg
     1681 cgcgcggctg acctatgcac tcgaagccga tgccgcgacc cgcgcgctgt ggccgcaggc
     1741 cttccgctgc gaactcgaac tgctcgcggc cggcgacacg ctggccgtga cgctggccgt
     1801 gcacaacacc ggcgaggcgc cgctgtcgtt caccggcgcg ctgcatacct acttcgccgt
     1861 caccgacctc gacgccgccc gcgtcagcgg tctggaagcg catccgtatc gcgaccacgc
     1921 cggtgcggcg gcagccggtg gcggcatcga ccattcgccc gatggcgcgc cgctttgctt
     1981 cggcgccgaa gtcgatcgcg tgtacgccgg cgtgcgcgga gcgatccggc tcgcgaccgg
     2041 tgccggcacg ctgcagatcg aggccgacgg ctttccggac gccgtgatct ggaaccccgg
     2101 gccggagaaa ggcgcagcac tcgccgatct cgaagccggg gcctggcgcc gctacgtctg
     2161 cgtcgaggcg gccatcgtgc gcacgccggt gtcggtcgcc gccggtgcgc tctggcgcgg
     2221 cacgcagcgg gtccgcgtga tttgacggac gccgcgaccg ccgtaccgtc gcgcgcggcg
     2281 atcctgcgct tcgcggcgcc ggtgatgctc gccaacctca cgacgccgct gctcggcatc
     2341 gtcgacaccg cgatcatggg ccgttcgccc gatccggcgc tgatcgccgc ggtcgcggtc
     2401 ggctcgacgg tgctgaccgt gctgttctgg gccttctcgt tcctgcgcca gagcacggtc
     2461 ggcctgaccg cgcaggcgct cggcgccggc gaggccacgg agctggccgc ggcgctgctg
     2521 cgcccgctgg cactggcggc gctgatcggc acggtgctga tcctgctgca ggcaccgatc
     2581 gcggcgctgg ccttcgggct gatgacgagt gctcccgaag tcgaaaccgc ggcacgcgcc
     2641 tattacggct ggcgcatcgc cgccgcgccg ttcgcgcttg ccaattacgc actcgccgcc
     2701 tggctgatcg gccagcgccg ggcgcgcctc gtgctcggcc tgcagctggc gatgaacggc
     2761 ggcaacgccg cgctgaccgc gctgttcgtg ctcggcttcg gcctcggccc ggccggggcc
     2821 ggactcggca ccgcgctcgc cgagatgctg acctgtctgg cgacgctggg gctgccggcc
     2881 gtgcgcgcgc agttcgcacc ggccgtgctg cgggcggtgt gcagcggcgg gcgcgcgcag
     2941 ttcgcgcggc tgatgcgcgc acgcgcggcc ggggtcgcgt cggtcgacgg cg
//
LOCUS       DAOQJH010000342         2975 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_22440_length_2975_cov_4.106507, whole genome shotgun sequence.
ACCESSION   DAOQJH010000342 DAOQJH010000000
VERSION     DAOQJH010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2975)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2975)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2975
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22440_length_2975_cov_4.106507"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..523)
                     /locus_tag="PLQ18_08375"
     CDS             complement(<1..523)
                     /locus_tag="PLQ18_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012486458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNE01706.1"
                     /translation="MAELPEPTSETLAPAAGNAPVRRVTVTGEKLRGADKVARIPVKI
                     APTTLETRLKKPEWIRARFPGSPEVQRLKGILRENRLHTVCEEASCPNLGECFGHGTA
                     TFMIMGDICTRRCPFCDVGHGRPNPLDPDEPLNMARTIAAMGLKYVVITSVDRDDLRD
                     GGAAHFAECIRRAR"
     gene            complement(525..1187)
                     /gene="lipB"
                     /locus_tag="PLQ18_08380"
     CDS             complement(525..1187)
                     /gene="lipB"
                     /locus_tag="PLQ18_08380"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:NF010922.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HNE01707.1"
                     /translation="MRSWWRRDLGRRSYAPVYAAMKAWTTARTADAGDEVWVVEHPPV
                     FTQGLNGKAEHLLATGDIPVVAVDRGGQVTYHGPGQIVVYPLLDIERAGIGVRTLVDA
                     LEGAVVDLLAARGIAACAKPDAPGVYVDGRKIASLGLRVRRGRCYHGLAVNIDMDLAP
                     FQRINPCGYAGLQMTQLSELCDDFAIQKATDELLHHLASRLGFADPLPLSELPTALSE
                     DA"
     gene            complement(1193..1462)
                     /locus_tag="PLQ18_08385"
     CDS             complement(1193..1462)
                     /locus_tag="PLQ18_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005962779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 domain-containing protein"
                     /protein_id="HNE01708.1"
                     /translation="MSEAPSDSLLEFPCDFPIKAFGEGDDFEALIVGLVRTHAPDLDP
                     ARVRVAASRTGRYIAVTVVVPAHSRAQLDAIYRDISGHARVKMAL"
     gene            complement(1509..2669)
                     /locus_tag="PLQ18_08390"
     CDS             complement(1509..2669)
                     /locus_tag="PLQ18_08390"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010487247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HNE01709.1"
                     /translation="MRLSVALRFLLSFVLLFGPGAAALAVDAVPAAPDLPVRAYVLVD
                     YASENVLAGKLADERIEPASITKLMAAYVIYQSIHEGKLKLDDQVLISENAWRTEGSR
                     MFAEVNSRVPADALLMGMVIQSGNDATVALAEHIAGSETTFVDLMNKWAKQLGLTRSH
                     FMNATGLPHPDHYMTAHDIAKLARALIHDFPEQYARYAIKEFTYNNITQHNRNRLLWL
                     DPSVDGVKTGHTSSAGYCLVTSAKREQGRMIAVVLGAKAEKDRVSASQALLNYGFSFY
                     TTRLMHEAGVALAEPRVWLGDTEKLPLGLAEPLGVTVPKGKAEGLKSEIQVDPQIEAP
                     VGRGSVHGKVVVTLDGNTVAEAPLVALQDVAEGSFFSRLLDHILMLFHKLIG"
     gene            complement(2762..>2975)
                     /locus_tag="PLQ18_08395"
     CDS             complement(2762..>2975)
                     /locus_tag="PLQ18_08395"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HNE01710.1"
                     /translation="QLGAFGDRRNAEALKTRIAGRFAQPVTIDGSRNDGLLRVKIGPF
                     QTEPEAERASLMVASLGLGRPRMVID"
BASE COUNT          459 a          965 c         1076 g          475 t
ORIGIN      
        1 cgcgcgcgcg ccggatgcat tcggcgaaat gcgcggcacc gccgtcgcgc aggtcatcgc
       61 ggtcgaccga ggtgatgacg acgtacttca ggcccatcgc cgcgatcgtg cgtgccatgt
      121 tcagcggctc gtccggatcg agcgggttcg gccggccgtg gccgacatcg cagaacgggc
      181 agcggcgcgt gcagatgtcg cccatgatca tgaaggtcgc cgtgccgtgg ccgaagcatt
      241 cgccgaggtt cgggcacgag gcttcctcgc agaccgtgtg caggcggttc tcgcgcagga
      301 tgcccttgag gcgctgcacc tccggcgaac ccgggaagcg tgcgcggatc cattccggct
      361 tcttcaggcg cgtttccagc gtggtcgggg cgatcttgac cgggatgcgc gcgaccttgt
      421 cggcgccacg cagcttctcg ccggtcaccg tgacgcggcg gacgggagcg ttgcccgcag
      481 cgggggcaag ggtttcggaa gtcggttcgg gcagctcggc catctcaggc gtcctcggac
      541 aaggcggtcg ggagttccga aagcggcagc ggatcggcga agcccagccg ggacgccaga
      601 tgatgcaata attcgtccgt cgctttctgt atcgcgaaat catcgcacag ctcggacagc
      661 tgtgtcatct gcaggccggc gtacccgcag gggttgatgc gctgaaacgg cgcgagatcc
      721 atgtcgatgt tgacggcgag tccgtgatag cagcgcccgc ggcgcacgcg caggccgagc
      781 gaggcgatct tgcgcccgtc gacgtagacc ccgggcgcat cgggcttcgc gcaggccgcg
      841 atgccgcgcg cggcgagcag gtcgacgacc gcgccttcga gcgcatcgac cagcgtgcgc
      901 acaccgatgc cggcgcgttc gatgtcgagc agcggataga cgacgatctg ccccggcccg
      961 tgataggtga cctgcccgcc gcgatcgacc gcgaccaccg ggatgtcgcc ggtcgcgagc
     1021 aggtgctcgg ccttgccgtt gaggccttgc gtgaacaccg gcgggtgttc gaccacccag
     1081 acctcgtccc cggcatcggc cgtgcgcgcc gtggtccaag ccttcatcgc cgcgtagacc
     1141 ggcgcgtagg aacgccggcc gagatcgcgc cgccaccagc tgcgcagcgg cttcacagcg
     1201 ccatcttcac gcgggcgtgg ccgctgatgt cgcggtagat cgcgtcgagc tgcgcgcgcg
     1261 agtgcgccgg cacgacgacg gtcacggcga tgtagcggcc ggtgcgcgag gcggcgacgc
     1321 gcacgcgcgc cgggtcgaga tcgggcgcgt gcgtgcgcac gagcccgacg atcagggctt
     1381 cgaaatcatc gccttcgccg aaggccttga tcgggaagtc gcagggaaat tcgagcagcg
     1441 agtcgctcgg cgcttcggac atggcaatca ccagcgggcg tgaccccgcc cgcaggcggg
     1501 ggcgggggtc agccgatcag cttgtggaac agcatcagga tgtgatcgag cagccgcgag
     1561 aagaagctgc cctcggccac gtcctgcagc gcgaccagcg gcgcctcggc gaccgtgttg
     1621 ccatcgagcg tgacgacgac cttgccgtgc actgaaccgc ggccgaccgg cgcctcgatc
     1681 tgcggatcga cctggatctc ggacttcagg ccctcggcct tgcccttggg caccgtgacg
     1741 ccgagcggct cggcgaggcc gagcggcagc ttttcggtat ccccgagcca gacgcgcggt
     1801 tcggccagcg ccacaccggc ctcgtgcatc agccgcgtcg tgtagaagct gaagccgtag
     1861 ttcagcagcg cctgactggc actgacccgg tccttctcgg ccttggcccc gagcacgacc
     1921 gcgatcatgc gtccctgctc gcgcttggcc gaggtcacga ggcagtagcc ggcgctgctg
     1981 gtgtggccgg tcttgacgcc atcgacgctc gggtcgagcc acagcaggcg gttgcggttg
     2041 tgctgggtga tgttgttgta cgtgaattcc ttgatcgcat agcgcgcgta ctgctcgggg
     2101 aagtcatgga tcagcgcgcg cgcgagcttg gcgatgtcgt gcgccgtcat gtagtgatcc
     2161 ggatgcggca ggccggtcgc gttcatgaag tgcgagcgtg tgaggccgag ctgcttcgcc
     2221 cacttgttca tcaggtcgac gaaggtcgtc tcggagccgg cgatgtgctc ggcgagtgcg
     2281 acggtcgcgt cgttgccgga ctggatgacc atgcccatca gcagcgcatc ggccggcacg
     2341 cgcgagttga cctcggcgaa catgcgcgag ccttcggtgc gccaggcgtt ctcgctgatc
     2401 agcacctgat cgtcgagctt gagcttgccc tcgtggatcg actggtagat cacgtaggca
     2461 gccatcagct tggtgatgct ggccggctcg atgcgttcgt cggcgagctt gccggcgagc
     2521 acgttctcgc tggcgtagtc gacgagcacg taggcccgga ccggcagatc cggggcggcg
     2581 ggcacggcat cgacggcgag ggcggctgcg ccgggtccga acagcagcac gaaggacaac
     2641 aggaatcgca aagcgacgga caggcgcatg ggcagatcga taccgggaaa cggaggaaac
     2701 gggggcggat tctagcgccc gccgccggac ggcggccgga ttgttgcggc gcgcaaacgc
     2761 atcagtcgat gaccatgcgc ggccggccga ggccgaggct cgcgaccatc aggctcgcgc
     2821 gctcggcttc gggctcggtc tggaacggcc cgatcttgac gcgcagcagg ccgtcgttgc
     2881 gcgagccgtc gatggtgacc ggctgcgcga agcgtccggc gatgcgggtc ttcagcgcct
     2941 cggcgttgcg ccggtcaccg aaggcgccga gctgc
//
LOCUS       DAOQJH010000343         2969 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_22537_length_2969_cov_3.537062, whole genome shotgun sequence.
ACCESSION   DAOQJH010000343 DAOQJH010000000
VERSION     DAOQJH010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2969)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2969)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2969
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22537_length_2969_cov_3.537062"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            103..513
                     /locus_tag="PLQ18_08400"
     CDS             103..513
                     /locus_tag="PLQ18_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017842820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE01711.1"
                     /translation="MSLKATWYRDKLLKKFRRGFHGYPVATVAYYGPDDRLASKAVVG
                     IAPDENAEVEMLERWYAETGDIRQDPAISEAIVRYIESHAVRSVLTPGRIIGCPHEAG
                     VHYPEGGTCPVCTYWAGRDRFTGERLDGEKESDA"
     gene            complement(632..1765)
                     /locus_tag="PLQ18_08405"
     CDS             complement(632..1765)
                     /locus_tag="PLQ18_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017938003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNE01712.1"
                     /translation="MRSPPLRNVLELMRREFYALGRDRVMLGLIVYAFSVLIYVNATA
                     TGIELRNAAIAFVDEDASTLSARLFDAPQPPYFQHPQRLALADVDAALDAGRFTFVVD
                     VPPGFQSDVLAGRAPRLQINVDATAMSQAGIGANYLQQIFSAEIDEFVRGRRQEAPQA
                     VTQVVRIAFNPNLQMPWFMGPMMIVNSITLLSIVLTGAALIREREQGTLEHLLVLPLT
                     PIEIMLAKIGATALVIVVATTFATVVMIHGVLSVPARGSLALFLVAAFVYSASIAAIG
                     IFLATLARSMPQLGLLSIPVVFPMAMLSGGATPLDSMPVAVQRIMQFSPSTHFTAIVQ
                     AILYRGAGFDVIWPQFVAMLAIGAVFLAIAVHRLRTTLAQAGS"
     gene            complement(1768..2820)
                     /locus_tag="PLQ18_08410"
     CDS             complement(1768..2820)
                     /locus_tag="PLQ18_08410"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNE01713.1"
                     /translation="MLGTLLLMLVMGYGISLDVEDLPYAVLDLDRTPESRAYLDSFKG
                     IRELAERAPATSSGDIDARLLAGELSFALELPPGFGAELRAGRRPEVGVRLDGAIPFR
                     AETARGYLLGIHAGFLADPRPAQLGPAPAETQTPLAGLEVRFRYNQDFRSVNAIVPGV
                     LALLLLVIPATLTALGVVREKELGSITNLYASPATRLEFLLGKQMPYAGLGLVNAAVM
                     ALLTVWLFGVPLKGSLPAFALGALLYVIAASAFGLLVSTVTSTQIAALFGTFILTMLP
                     SINFSGLIAPVSSMSGGAAVMGHSFPASYFLAIAVGTFTKALGFAALAPKYLQLTGFT
                     LVYVLLAWRLLPKQER"
     gene            2789..>2969
                     /locus_tag="PLQ18_08415"
     CDS             2789..>2969
                     /locus_tag="PLQ18_08415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE01714.1"
                     /translation="MTSISSSVPSSAKARRIGSRRSSSASRHAKVSSLRSEKPWPARS
                     SSPPADDSPAGPHPRV"
BASE COUNT          514 a         1010 c         1059 g          386 t
ORIGIN      
        1 tcatgcggtc agccgatgac gcagccggag cgcgggcgtg cgcgacgacg ggcggctcgg
       61 gtaccgtccc gctctccctc cccgcacgac gaggcacgca cgatgtcgct caaggccact
      121 tggtatcggg acaagctgct gaagaagttc cgccgcggtt tccacggcta tccggtcgcg
      181 accgtcgcct attacggccc cgacgaccgg ctggcgagca aggccgtggt cggcatcgcc
      241 ccggacgaga acgccgaggt cgagatgctg gagcgctggt acgcggaaac cggcgacatc
      301 cgccaggatc cggcgatcag cgaagccatc gttcgctaca tcgaaagcca tgcggtgcgg
      361 tcggtgttga cgccgggccg catcatcggc tgcccgcacg aggccggcgt gcattatccc
      421 gagggcggga cctgcccggt ctgcacctac tgggcagggc gcgaccggtt caccggcgaa
      481 cggctggacg gggagaagga aagcgacgcc tgagcggcag cggccggcag ggcagcgcgg
      541 ttcatgccgg atggccgcgc gacggcatcc gcagggcctg gtcgccgatc ggctccggac
      601 gccgggggac agccgctgac gatccggcgt ttcagcttcc cgcctgcgcc agcgtcgtcc
      661 tgagccggtg caccgcgatc gcgagaaaca cggcaccgat cgcgagcatc gcgacgaact
      721 gcggccagat cacgtcgaat ccggccccgc ggtacaggat cgcctgcacg atcgccgtga
      781 agtgcgtcga cggcgagaac tgcatgatcc gctgcaccgc aaccggcatg ctgtcgagcg
      841 gcgtggcgcc gcccgagagc atcgccatcg ggaacaccac cgggatcgac agcagcccga
      901 gctgcggcat cgagcgcgcc agcgtcgcga ggaagatgcc gatcgccgcg atcgaggcgc
      961 tgtagacgaa ggccgcaacc aggaacagcg cgagcgagcc gcgcgccggc accgacagca
     1021 cgccgtggat catcacgacg gtcgcaaacg tcgtggcgac gacgatcacc agcgccgtcg
     1081 cgccgatctt cgcgagcatg atctcgatcg gcgtcagcgg cagcaccagc aggtgttcga
     1141 gcgtgccctg ctcgcgctcg cggatcagcg ccgcgccggt cagcacgatc gacagcagcg
     1201 tgatgctgtt gacgatcatc atcggcccca tgaaccacgg catctgcaga ttcgggttga
     1261 aggcgatgcg cacgacctgc gtgaccgcct gcggcgcctc ctgccggcgt ccgcgcacga
     1321 actcgtcgat ctcggcgctg aagatctgct gcaggtagtt cgcgccgatg ccggcctggc
     1381 tcatggccgt ggcatcgacg ttgatctgca gccgcggcgc gcgtccggcg agcacgtcgc
     1441 tctggaagcc gggcggcacg tcgacgacga aggtgaagcg cccggcgtcg agcgcggcat
     1501 cgacatcggc gagtgccaga cgctgcgggt gctggaagta cggcggctgc ggcgcatcga
     1561 acagccgcgc cgacagcgtg ctcgcgtcct cgtcgacgaa ggcgatcgcg gcgttgcgca
     1621 gctcgatgcc ggtggccgtg gcattcacgt agatcagcac gctgaacgcg tagacgatca
     1681 gtccgagcat gacgcggtcg cgcccgagcg cgtagaactc gcggcgcatc agttcgagca
     1741 cgttgcgaag cggcggcgac cgcacgctca gcgctcctgc ttcggcagca gccgccacgc
     1801 gagcagcacg tagaccagcg tgaagccggt gagctgcagg tacttcggtg ccagcgccgc
     1861 gaagccgagc gccttcgtga aggttccgac cgcgatcgcg aggaagtagc tggccgggaa
     1921 ggagtgcccc atcactgctg cgccgccgct catcgacgac accggcgcga tcaggcccga
     1981 gaagttgatc gacggcagca tggtcaggat gaaggtgccg aacagcgccg cgatctgcgt
     2041 gctcgtgacc gtcgagacca gcagcccgaa ggccgaggcc gcgatcacgt acagcagcgc
     2101 cccgagcgcg aaggcgggga ggctgccctt cagcggcacg ccgaacagcc acacggtcag
     2161 cagcgccatc accgcggcgt tgaccagtcc gaggccggcg taaggcatct gcttgccgag
     2221 caggaattcg agccgcgtcg ccgggctcgc gtacaggttg gtgatcgagc cgagttcctt
     2281 ctcgcgcacg acgccgagcg cggtcagcgt cgccgggatc accagcagca gcagcgccag
     2341 caccccgggc acgatggcgt tgaccgagcg gaagtcctgg ttgtagcgaa agcgcacttc
     2401 gagcccggcg agtggcgtct gcgtctcggc cggtgccggc ccgagctggg ccggacgcgg
     2461 atcggcgagg aagccggcgt ggatgccgag caggtagccg cgcgcagtct cggcgcggaa
     2521 cgggatcgcg ccgtcgaggc gcacgccgac ctcgggccgg cgcccggcgc gcaactcggc
     2581 gccgaagccg ggcggaagct ccagcgcaaa gctcagctcg ccggcgagca gccgcgcatc
     2641 gatgtcgccg gacgaggtcg ccggggcgcg ttcggccagt tcgcggatgc ctttgaagct
     2701 gtcgaggtag gcgcggctct cgggcgtgcg gtcgagatcg agcacggcat agggcaggtc
     2761 ctcgacatcg agcgagatgc cgtagcccat gaccagcatc agcagcagcg tgccgagcag
     2821 cgcgaaggcg aggcggatcg ggtcacggcg cagctccagc gcctcgcgcc acgcgaaggt
     2881 cagcagcctg cgcagcgaga agccgtggcc ggcacgcagc tcctcgccgc ccgccgatga
     2941 ctcacctgcc ggacctcacc ctcgagtgg
//
LOCUS       DAOQJH010000344         2965 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_22587_length_2965_cov_4.217869, whole genome shotgun sequence.
ACCESSION   DAOQJH010000344 DAOQJH010000000
VERSION     DAOQJH010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2965
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22587_length_2965_cov_4.217869"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..630)
                     /gene="sucD"
                     /locus_tag="PLQ18_08420"
     CDS             complement(<1..630)
                     /gene="sucD"
                     /locus_tag="PLQ18_08420"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005958515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HNE01715.1"
                     /translation="MSILIDKNTKLICQGFTGSQGTFHSEQCLAYGTNLVGGVSPGKG
                     GSAHLGKPVFNTVHEAVRDAGANASMIYVPAPFAADAILEAADAGIKVIACITEGIPV
                     LDMLKVKAALKGSDVRLIGPNCPGLITPGECKMGIMPGHIHQPGKIGIVSRSGTLTYE
                     AVHQTTRIGLGQSTCVGIGGDPIHGMSFIDVLEMFEADSATKGIVMVGEI"
     gene            complement(644..1804)
                     /gene="sucC"
                     /locus_tag="PLQ18_08425"
     CDS             complement(644..1804)
                     /gene="sucC"
                     /locus_tag="PLQ18_08425"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HNE01716.1"
                     /translation="MNLHEYQAKELFRQFGVAVPRGIKATSPKEAVAAAKELGGSVWV
                     VKAQVHAGGRGKAGGVKLARSLQEVEDIAGSLLGTRLATKQTGPEGLPIHALLIEEGL
                     NIVDELYLSVLVDRSTKRIAFIGSSEGGMDIEEVAESTPEKILTVAIDPAAGFQAYEA
                     RQLGFGMGLAKPQVDHLVKVMNGLYDMFVAKDASLVEINPLIVTGEGKLLALDAKIGL
                     DDNALYRHKDIAEWRDSTQEDERENLAHEIGLNYVALDGNIGCMVNGAGLAMATMDMV
                     KLHGGEPANFLDVGGGATAERVAKAFKLILSSDKVRAIFVNIFGGIVRCDLIAEGIIA
                     AVKEVGLTIPVVVRLQGTNVEAGRKMLSESGMNIIAADDLTEAAKAAVAAAK"
     gene            2232..>2965
                     /locus_tag="PLQ18_08430"
     CDS             2232..>2965
                     /locus_tag="PLQ18_08430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE01717.1"
                     /translation="MDRRRGWSVLRAYVLYRLVLATLLLGITLAELAGKLFGAEDPRL
                     YWWTIGSYFGLLATGFLLSYLRRPDLIVQATLHAGLDIVVFTALMYASGGASGSLGAL
                     LVSAIAGSAILLPRRLVITVTAAAALAIIGVWLYGIWQSFALRTGAPYLSLDLLPPLV
                     DWALVHGASGGQTALICLALAAAAAAAYTIAERTRNSETLVLERTQELLEMAELNAAI
                     IRHLQSGILVVDRHARGDRRAGVGVG"
BASE COUNT          428 a         1023 c          994 g          520 t
ORIGIN      
        1 gatctcgccg accatgacga tgcccttggt ggcgctgtcg gcctcgaaca tctccagcac
       61 gtcgatgaag ctcatgccgt ggatcgggtc accgccgatg ccgacgcagg tcgactggcc
      121 gagaccgatg cgcgtggtct ggtgcacggc ctcgtaggtc agcgtgcccg agcgcgagac
      181 gatgccgatc ttgcccggct ggtggatgtg gccgggcatg atgcccatct tgcactcgcc
      241 cggggtgatc aggccggggc agttcgggcc gatcagccgg acgtccgagc ccttcagcgc
      301 ggccttgacc ttcagcatgt cgaggaccgg gatgccctcg gtgatgcagg cgatgacctt
      361 gatgccggcg tcggcggctt cgaggatcgc atcggccgcg aacggcgccg gcacgtagat
      421 catcgaggcg ttggcaccgg cgtcgcgcac ggcttcatgg acggtgttga acaccggctt
      481 gccgagatgg gccgaaccgc ccttgcccgg gctgacacca ccgaccaggt tggtgccgta
      541 ggccaggcac tgctcggagt ggaacgtacc ctgcgaaccc gtgaagccct ggcagatcag
      601 cttggtgttc ttgtcgatca ggatgctcat gtaactcccc ctctcacttc gcggcggcga
      661 cggcggcctt ggcggcttcg gtcaggtcgt cggcagcgat gatgttcatg ccgctttccg
      721 acagcatctt gcggccggcc tcgacgttcg tgccctgcag gcgcacgacg acgggaatgg
      781 tcaggccgac ttccttgacc gcggcgatga tgccttcggc gatcaggtcg cagcggacga
      841 tgccgccgaa gatgttgacg aagatcgcgc ggaccttgtc cgaggagagg atgagcttga
      901 acgccttggc gacgcgctcg gccgtggcac cgccgccgac gtcgaggaag ttcgccggct
      961 cgccgccgtg cagcttgacc atgtccatgg tcgccatcgc cagaccggcg ccgttgacca
     1021 tgcagccgat gttgccgtcg agcgcgacgt agttcaggcc gatctcgtgc gcgaggttct
     1081 cgcgctcgtc ttcctgcgtc gaatcgcgcc actcggcgat gtccttgtgg cggtacagcg
     1141 cgttgtcgtc gaggccgatc ttggcatcga gcgcgagcag cttgccctcg ccggtgacga
     1201 tcagcgggtt gatctcgacc agcgaggcgt ccttggcgac gaacatgtca tagagaccgt
     1261 tcatgacctt gaccagatga tcgacctgcg gcttggcgag tcccatgccg aagcccagct
     1321 ggcgggcctc gtaggcctgg aagccggcgg ccggatcgat ggcgacggtc aggatcttct
     1381 cgggcgtgct ttcggcgact tcctcgatgt ccatgccgcc ttcggaagaa ccgatgaacg
     1441 cgatgcgctt ggtgctgcga tcgaccagca ccgacaggta cagctcatcg acgatgttca
     1501 ggccttcctc gatcagcagt gcatggatcg gcaggccctc gggaccggtc tgcttggtcg
     1561 cgagccgggt gccgagcagc gagccggcga tgtcctcgac ctcctgcagg ctgcgcgcca
     1621 gcttgacgcc accggccttg ccgcggccgc ccgcgtgcac ctgcgccttg accacccaca
     1681 cggagccccc cagctccttg gcggcggcca cggcttcctt cggcgaagtt gccttgatgc
     1741 cgcgcggcac cgcgacgccg aactggcgga aaagctcctt tgcttggtat tcgtgcagat
     1801 tcatgcgaat tccaccccct ttcgatagga gccggtatcc tatcggacgg cgccacaaaa
     1861 gcctatcggc cgccgcccgt ccgcaagcgt gttgcgcgca ccgcccatga gtcgatacct
     1921 gctgctcctg ctgctcgtcc tcgccgccgt ctggtggctg cgccgcgccc ggcgcccgca
     1981 agccccggca aggccgccgg caccggcaca accggcaaaa cctgactggc ctgcaatggt
     2041 gcgctgcacg gagtgcggca tacacttccc ggcaaccgat gcggtgcgcg atgccgatgc
     2101 gcgtccgtat tgctgcccgg cgcaccgcga gcgccatgcg ctgcgcccac ccaaggactg
     2161 aacggccgcc gccgcacggg cgccggcccc gcctgaggcc ctgctgaacg atgatcgtta
     2221 cggacagcgt catggaccgg cgacgcggct ggtcggtgct gcgcgcctac gtgctgtacc
     2281 gcctggtgct ggccacgctg ctgctcggca tcacgctcgc cgagctggcc ggcaagctgt
     2341 tcggtgccga agacccgcgc ctttactggt ggacgatcgg cagctatttc ggcctgctcg
     2401 ccaccggctt cctgctgagc tacctgcgcc ggcccgacct catcgtgcag gcgacgctgc
     2461 acgccggcct cgacatcgtc gtgttcacgg cgctgatgta cgcaagcggc ggggcttccg
     2521 gcagcctcgg ggcgctgctg gtgtcggcga tcgccggcag cgcgatcctg ctgccgcgcc
     2581 ggctggtgat cacggtcacg gccgcggcgg ccctcgcgat catcggcgtc tggctgtacg
     2641 gcatctggca gagcttcgca ctgcgcaccg gcgcgccgta cctgagcctc gacctgctgc
     2701 cgccgctggt ggactgggcg ctcgtgcatg gcgcctccgg cggccagacg gcgctgatct
     2761 gtctggcgct ggccgccgcc gccgccgccg cgtacacgat cgccgagcgc acgcgcaaca
     2821 gcgagaccct ggtgctcgaa cgcacgcagg agctgctgga gatggcggag ctgaacgcgg
     2881 cgatcatccg ccacctgcag tccggcatcc tcgtcgtcga tcgtcacgcg cgcggggatc
     2941 ggcgcgccgg tgtcggcgtc ggtca
//
LOCUS       DAOQJH010000345         2965 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_22589_length_2965_cov_3.976976, whole genome shotgun sequence.
ACCESSION   DAOQJH010000345 DAOQJH010000000
VERSION     DAOQJH010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2965
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22589_length_2965_cov_3.976976"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..787)
                     /locus_tag="PLQ18_08435"
     CDS             complement(<1..787)
                     /locus_tag="PLQ18_08435"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010961561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HNE01718.1"
                     /translation="MAMTPSSIQLDADGRLRHFLTVEGLKRRHLVEILDRAEALSGVA
                     ERRMTKSPTLHGKTVVNLFFENSTRTRTTFELAAKRLSADVLNVNISTSSTHKGETLL
                     DTIRNIEAMQVDMFVVRHAVSGAAEFIARHVAPHIAVLNAGDGRHAHPTQAMLDMMTI
                     RRHKGEFANLRVAIVGDVLHSRVARSDIHALNILGTGEVRVVAPKTLLPADVGTLGVH
                     VFHDLEHGLRGVDVVIMLRLQRERMSGALLPSASEYFQQYGLTA"
     gene            complement(940..1413)
                     /gene="pyrR"
                     /locus_tag="PLQ18_08440"
     CDS             complement(940..1413)
                     /gene="pyrR"
                     /locus_tag="PLQ18_08440"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015475137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HNE01719.1"
                     /translation="MQLDAERLIDTLASGLRAHLARRGIGTPLLFGIRTGGVGLAERL
                     RVALGVAEPIGTLDISFHRDDDVRDGLDPQVRPSRIPVDPEGRDLVLVDDVLYTGRTV
                     RAALNELFDYGRPRSVTLAVLVDRGGRELPIQADVVAELVTAAPGERVELRWPGG"
     gene            complement(1389..1844)
                     /gene="ruvX"
                     /locus_tag="PLQ18_08445"
     CDS             complement(1389..1844)
                     /gene="ruvX"
                     /locus_tag="PLQ18_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003157748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HNE01720.1"
                     /translation="MPASAPRPRTVLAFDYGSVRIGVAVGSELTGSARPLATLAGRDW
                     TAIGRLIDEWRPDACVVGVPRHADGSESETTRGALRFARQLDGRYGLPVATHDERLSS
                     YAAAGRLAAAPASRRRPGPGLDAAAAALILESWFATVRNDPPCNSTPNA"
     gene            complement(1846..2406)
                     /locus_tag="PLQ18_08450"
     CDS             complement(1846..2406)
                     /locus_tag="PLQ18_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005963206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HNE01721.1"
                     /translation="MLEPSYLSHQFLIAMPALADPNFARTVTYLCEHGPQGALGLIVN
                     RPLDLTTADLLENLGIEAPPGTGALPVYFGGPVHPEQGFVLHRPLGRWQSTLATGDGL
                     AITTSRDILEAMARGQGPAEALIALGYAGWGPGQLERELAENAWLSCPADADVIFRLP
                     AAERWAAAAALLGVDLRLLCGDAGHA"
     gene            complement(2477..>2965)
                     /locus_tag="PLQ18_08455"
     CDS             complement(2477..>2965)
                     /locus_tag="PLQ18_08455"
                     /inference="COORDINATES: protein motif:HMM:NF024503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HNE01722.1"
                     /translation="PKPVPAPRPVAPPAPRRSGPLSAAELMGAGLDSIRQEAPLPAAS
                     SRGRSKRADPTDSSTLEGYYAAAWVAKVERIGALNFPEEARRRNLTGSLRLSVVIRAD
                     GTILETTVVRSSGQSALDEGARRIVELGAPYAPFPDELRRRYDTLVIVRDWQFRQGAQ
                     FQ"
BASE COUNT          432 a         1014 c         1121 g          398 t
ORIGIN      
        1 cggcggtcag gccgtactgc tggaagtatt cggaagccga gggcagcagc gcgccgctca
       61 tgcgctcgcg ctgcaggcgc agcatgatga ccacatcgac cccgcgcagc ccgtgttcga
      121 ggtcgtggaa cacgtgcacg ccgagcgtgc cgacatcggc cggcagcagc gtcttcggcg
      181 cgaccacgcg cacctcgccg gtgccgagga tgttcagcgc atggatgtcg gagcgcgcca
      241 cgcgcgagtg cagcacgtcg ccgacgatgg cgacgcgcag gttcgcgaat tcacccttgt
      301 ggcggcggat ggtcatcatg tcgagcatcg cctgcgtcgg atgcgcatgg cggccgtcgc
      361 cggcgttgag caccgcgatg tgcggcgcga cgtggcgggc gatgaactcg gccgcgcccg
      421 aaaccgcgtg gcgcacgacg aacatgtcga cctgcatcgc ctcgatgttg cggatggtgt
      481 cgagcagcgt ctcgcccttg tgcgtcgagg aggtgctgat gttgacgttc agcacgtcgg
      541 ccgacaggcg cttggcggcg agttcgaagg tcgtgcgcgt gcgcgtcgag ttctcgaaga
      601 acaggttgac gacggtcttg ccgtgcagcg tcggggactt ggtcatgcgc cgctcggcga
      661 cgcccgagag cgcctcggcg cggtcgagga tctcgaccag atgccggcgc ttcaggccct
      721 cgacggtcag gaagtggcgc agacgcccgt cggcgtcgag ctggatggac gatggagtca
      781 tggcgatgcc tggtcgaccc ggtgcgtgcg ccggcgccgt cgtcgggcga cagcggacgg
      841 ctcggaagct tcgatacggg atacagcggg aaacaacaaa attactgcgg aaagccgtgc
      901 cataccatcg cgacgatgca cgccgccgca gccggccgct cagccgcccg gccagcgcag
      961 ctcgacgcgc tcgccgggcg ccgccgtgac gagctcggca accacgtcgg cctggatcgg
     1021 cagctcgcgt ccgccgcgat cgaccagcac tgccagcgtc accgagcgcg gccggccgta
     1081 gtcgaacagt tcattgagcg ccgcgcgcac ggtgcggccg gtgtagagca cgtcatcgac
     1141 cagcaccaga tcgcggcctt cgggatcgac cgggatgcgc gagggccgca cctgcgggtc
     1201 gaggccgtcg cgcacgtcgt cgtcgcggtg aaacgagatg tcgagcgtgc cgatcggctc
     1261 ggcgacgccg agcgcgacgc gcaggcgctc ggcgagtccg accccgccgg tgcggatgcc
     1321 gaacagcagc ggcgtcccga tgccgcgccg ggccagatgc gcgcgcagcc cgctggccag
     1381 ggtgtcgatc aggcgttcgg cgtcgagttg catggcggat cgttcctgac ggtggcaaac
     1441 cagctttcga ggatcagggc ggcggcggcg gcatcgagcc ccggaccggg ccggcggcgc
     1501 gaggccgggg cggctgccag acgtccggcg gcggcgtacg aggacaggcg ctcgtcgtgg
     1561 gtcgcgaccg gcaggccgta gcggccgtcg agctggcgcg cgaagcgcag cgccccgcgc
     1621 gtggtttcgg attcgctgcc gtcggcgtgg cgcggcacgc cgacgacgca ggcatcgggc
     1681 cgccattcgt cgatcagccg gccgatggcc gtccagtcgc ggccggcgag cgtcgcgagc
     1741 ggccgggcgc tgccggtcag ctccgacccg accgcgacgc cgatgcgcac gctgccgtag
     1801 tcgaaggcca gcacggtccg tggccgcggt gcgctcgcgg gcacgtcagg cgtgaccggc
     1861 gtcgccgcag agcaggcgca gatcgacgcc gagcagcgcc gcggccgccg cccagcgctc
     1921 ggccgccggc agccggaaga tcacgtcggc atcggccggg caggacagcc aggcgttctc
     1981 ggcaagttcg cgttcgagct ggcccggccc ccagccggcg tagccgagcg cgatcagcgc
     2041 ctcggccggt ccctgaccac gggccatggc ttcgaggatg tcgcgcgagg tggtgatcgc
     2101 gagtccgtcg ccggtcgcga gcgtcgactg ccagcgcccg agcggccgat gcagcacgaa
     2161 gccctgctcc ggatggaccg gcccgccgaa gtagaccggc agcgccccgg tgccgggcgg
     2221 cgcctcgatg ccgaggtttt cgagcaggtc ggcggtggtg agatcgagcg gccggttcac
     2281 gatcagcccg agcgccccct gcggtccgtg ctcgcagagg taggtcacgg tgcgcgcgaa
     2341 gttcggatcc gcgagcgccg gcatcgcgat caggaactga tggctgagat acgagggttc
     2401 gagcatggcg gcagtatcgt gagcgtccgg gacttccaca agacacgacg gtgcggcacg
     2461 cgggcgcaag cgcccgtcac tggaactgcg caccctggcg gaactgccag tcgcgcacga
     2521 tcaccagcgt gtcgtagcgc cggcgcagct catcgggaaa cggcgcgtac ggcgcgccga
     2581 gctcgacgat gcgccgcgcg ccctcgtcga gcgccgactg gcccgaggag cgcacgacgg
     2641 tggtttcgag gatcgtgcca tcggcgcgga tcaccaccga caggcgcagc gaaccggtca
     2701 ggttgcgtcg gcgggcttcc tcggggaagt tcagcgcgcc gatgcgctcg accttcgcga
     2761 cccaggctgc ggcgtaatag ccttccagcg tcgagctgtc ggtcggatcg gcgcgcttgc
     2821 tgcgcccgcg gctgctcgcc gccggcagcg gggcctcctg acggatcgag tcgagaccgg
     2881 cgcccatcag ctcggcggcg ctcagcggtc cgctgcgccg cggcgccggc ggcgccaccg
     2941 ggcgcggggc cggcacgggt ttcgg
//
LOCUS       DAOQJH010000346         2964 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_22603_length_2964_cov_3.825713, whole genome shotgun sequence.
ACCESSION   DAOQJH010000346 DAOQJH010000000
VERSION     DAOQJH010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2964)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2964)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2964
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22603_length_2964_cov_3.825713"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1743
                     /locus_tag="PLQ18_08460"
     CDS             <1..1743
                     /locus_tag="PLQ18_08460"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNE01723.1"
                     /translation="SLLATETLEAAGGQLAVFNEDTATALGKVVPRGTRIENPLDLGD
                     AANPERYIAVLDALLEAPGVDCILVLHSPLSPELDLASAKAVADRAAKSRRLVITSWL
                     GAASAEPARAVFKQAHVATYDNPGDAVSAFTRIAQHRHNLELLMETPPSIPEAFKPDA
                     ERARALLQQALAAGRMRLDAYESMQVLDAYGIPTVPTRVARTPEEAAAIYPEFGHVVV
                     LKLLSPDIASKTEVGALALWLDSAEAVRSEAEAMIARVRTHVPEARIEGFVVQPMLRR
                     GGAYELAIGMRTGDTFGPLIRFAHGGTEADVIDDKAWALPPLNMALAQEMIGRTRISQ
                     RLKSGLARAADLDALALALIKISQMAIDLGELWELDINPLRVSGKGIIALDATIQVRA
                     FTGKRPRDRLAIRPYPSELEQDFTLPDGRGLRLRPIVPEDEPALQELVRRMPPDELRL
                     RFFQPIRELSHAMAARLTQLDYYREMALIVAARDVPGRAEIYGVVRITCDPDMDRAEY
                     AILVDHGMTGLGLGPMLMRRIIDYARRRGIQEIYGEVLWENEGMLKLCRALGFAVKPS
                     ADDPGIMHVSIKVD"
     gene            complement(1730..2725)
                     /locus_tag="PLQ18_08465"
     CDS             complement(1730..2725)
                     /locus_tag="PLQ18_08465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE01724.1"
                     /translation="MVAVTAVTVRRTLRALLCCLVLAPVAPLRADGGANLYAALERLP
                     ATPVEVRGATLRVAFADDGFDLPRARLLDWVRRGAEAVAVYYDGFPVRDYRLLIEPVD
                     GRRVAGGTTWAWRGPASRVSVGRMADEAVLAHDWVLVHEMVHTALPLLDRRHHWLEEG
                     IASYVEPLARAQAGQLSEAQVWAQFVGGMPLGLPQDGDEGLDRTHTWARTYWGGALFC
                     LLADVAIREESGGRAGLREALRAIAKDSRGMADEWPIERVLAAGDAATGTGVLTRLYA
                     THASAAVSPDLPALWRALGVVAQENAEPRFDDSAPLAAIRRSLTAPAPAGVLSRP"
BASE COUNT          432 a         1125 c         1034 g          373 t
ORIGIN      
        1 agcctgctcg cgaccgaaac cctggaggcc gccggcggcc agctcgcggt cttcaacgag
       61 gacaccgcca ccgcgctcgg caaggtcgtg ccgcgcggca cgcgcatcga gaacccgctc
      121 gatctcggcg atgcggccaa ccccgagcgc tacatcgccg tgctcgatgc gctgctcgaa
      181 gccccgggcg tcgactgcat cctggtgctg cactcgccgc tgtcgccgga gctcgatctg
      241 gcgagcgcga aggccgtcgc cgatcgcgcc gcgaagagcc ggcggctggt gatcaccagc
      301 tggctcggtg cggcttcggc cgagcccgca cgcgcggtgt tcaagcaggc gcacgtcgcg
      361 acctacgaca accccggcga cgcggtcagc gccttcacgc gcatcgccca gcaccgccac
      421 aacctcgaac tgctgatgga gacgccgccg tcgatccccg aggccttcaa gcccgacgcc
      481 gaacgcgcgc gcgcgctgct gcagcaggcc ctcgccgccg ggcgcatgcg cctcgatgcc
      541 tacgagtcga tgcaggtgct cgatgcctac ggcatcccga ccgtgccgac gcgcgtcgcg
      601 cgcacgcccg aggaagccgc ggcgatctat ccggagttcg gccacgtcgt cgtgctgaag
      661 ctgctgtcgc ccgacatcgc cagcaagacc gaggtcggcg cgctggcact gtggctcgat
      721 tcggccgagg ccgtgcgcag cgaagccgag gcgatgatcg cgcgcgtgcg cacgcacgtg
      781 ccggaggccc gcatcgaagg cttcgtcgtg cagccgatgc tgcgccgtgg cggcgcctat
      841 gaactcgcca tcggcatgcg caccggcgac accttcgggc cgctgatccg cttcgcgcac
      901 ggcggcaccg aggccgacgt catcgacgac aaggcctggg cgctgccgcc gctgaacatg
      961 gcgctcgcgc aggagatgat cggccgcacg cgcatcagcc agcgcctgaa atcgggcctc
     1021 gcccgcgccg ccgacctgga tgcgctggcg ctggcgctga tcaagatctc gcagatggcg
     1081 atcgatctcg gcgagctctg ggagctcgac atcaatccgc tgcgcgtctc cggcaagggc
     1141 atcatcgcgc tcgatgcgac catccaggtg cgcgccttca ccggcaagcg cccgcgcgac
     1201 cggctggcga tccgccccta tccgagcgag ctggagcagg acttcacgct gcccgacggc
     1261 cgcggcctgc gcctgcgccc gatcgtgccc gaggacgagc cggcgctgca ggagctggtc
     1321 aggcgcatgc cgcccgatga actgcgcctg cgcttcttcc agccgatccg cgagctgtcg
     1381 cacgcgatgg ccgcgcgcct gacccagctc gactactacc gcgagatggc gctgatcgtc
     1441 gccgcgcgcg acgtgccggg ccgcgccgag atctacggcg tcgtgcgcat cacctgtgat
     1501 ccggacatgg accgcgccga atacgcgatc ctcgtcgatc acggcatgac cggcctcggc
     1561 ctcggcccga tgctgatgcg ccggatcatc gactacgcac gccggcgcgg catccaggag
     1621 atctacggcg aggtgctgtg ggagaacgag ggcatgctga agctctgccg tgcgctcggc
     1681 ttcgcggtca agccgagcgc cgatgatccc ggcatcatgc acgtcagcat caaggtcgac
     1741 tgagcacccc ggccggtgcc ggcgcggtca ggctgcgccg gatcgccgca agcggcgccg
     1801 agtcgtcgaa gcgcggctcg gcgttctcct gcgccacgac gccgagcgcg cgccagagcg
     1861 ccggcaggtc ggggctgacc gctgcgctgg cgtgtgtggc gtacaggcgc gtgagcacgc
     1921 cggtgccggt cgcggcatcg ccggccgcga gcacccgctc gatcggccat tcgtcggcca
     1981 tgccccggct gtccttcgcg atcgcccgca gcgcctcgcg cagcccggcg cggccgcccg
     2041 attcctcgcg gatcgccacg tcggcgagca ggcagaacag cgccccgccc cagtaggtgc
     2101 gcgcccaggt gtgcgtgcga tccagcccct cgtcgccgtc ctgcggcagc ccgagcggca
     2161 tgccgccgac gaactgcgcc cagacctgcg cctcgctgag ctgcccggcc tgcgcgcgcg
     2221 ccagcggctc gacgtagctg gcgatgcctt cctccagcca gtgatggcgc cggtcgagca
     2281 gcggcagtgc cgtgtgcacc atctcgtgca ccagcaccca gtcgtgcgcg agcacggcct
     2341 cgtcggccat gcgcccgacc gagacgcggc tcgccggccc gcgccaggcc caggtcgtgc
     2401 cgccggcgac ccggcgcccg tcgaccggct cgatcagcag ccggtagtcg cgcaccggaa
     2461 agccgtcgta atagaccgcg accgcctcgg ccccgcggcg cacccagtcg agcagccggg
     2521 cccggggcag gtcgaagccg tcatcggcga aggccacgcg cagcgtcgcg ccccgcacct
     2581 cgaccggcgt cgccggcagc cgctccagtg ccgcataaag gttcgcaccg ccgtcggcac
     2641 gcagcggcgc gaccggcgcg agcacgaggc agcagagcag cgcgcgcaga gtgcggcgca
     2701 cggtgacggc ggtgacggca accatcgcgg tgactcccgg ggactacggt gttcggaacg
     2761 ggcgacacgc ccggctggcc cacggggccg ggcgatgccc ggtgcccgcg cggcgaaacg
     2821 gctgcggacg cccgcggcgc ggcacgatac tgaaagcccg gcacgaccgg gagaccggag
     2881 accgcagcgg cgatgtacgc aggcccgcct gaagacgatg ccgcggaccg ggcccgggcc
     2941 ttccggctgc gttcgcgctg gcgg
//
LOCUS       DAOQJH010000347         2962 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_22623_length_2962_cov_4.162711, whole genome shotgun sequence.
ACCESSION   DAOQJH010000347 DAOQJH010000000
VERSION     DAOQJH010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2962)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2962)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2962
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22623_length_2962_cov_4.162711"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            426..1676
                     /locus_tag="PLQ18_08470"
     CDS             426..1676
                     /locus_tag="PLQ18_08470"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HNE01725.1"
                     /translation="MTPHRLAPAAAGLLLCSSLPAQATIVLQTLTDAVAAPMYAAATA
                     ADPAHLYVAERDGTVRRVERTSGAVEATPFLDIRSQVNTGGEGGLLGIAFDPGYAGNG
                     HFYVNYLDNSQQTRVVRYTAAGGVVDPGSAQTVIAISQPQLNHNGGWIGFGPDNQLYV
                     ATGDGGGSDDNDAGHTPGIGNAQDLSSPLGKILRLDVGSDAFPADPQRNYAIPADNPL
                     LGGAAAPSEIWAYGLRNPFRAGFDRLTGDLWIGDVGQLTREEIDFQPAGIGGQDYGWR
                     LREGTIATPSGGVGGPNPDAVDPVHEYDHTVGRSVIGGTVYRGSIAELYGRYFFADFN
                     AGRLWTLDGSGVTDWTDLLTAIGAPLLGIVGFSEDVDGELYLMDLGGAIYRIAEIPAP
                     GGLALLLAGLGLLRLRAARPPVLG"
     gene            1845..2960
                     /locus_tag="PLQ18_08475"
     CDS             1845..2960
                     /locus_tag="PLQ18_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019485564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HNE01726.1"
                     /translation="MTPAPTLFRTLLLAALVGVSGSAVADRVKDLASVGGVRSNQLIG
                     YGLVVGLAGTGDQTSQTPFTVQSLRNMLTGLGVVLPANISLQLKNVAAVSVHAELPPF
                     AKAGQTVDVTVSSLGNAKSLRGGSLLLSPLRGTDGEVYAIAQGNVVVGGLGVEGAGSS
                     ITVNVPSSGRIANGATVERPVPDGMTHAEGPLMLNLRQSDFTTAQRVADAINRKLHSQ
                     VAAPMDATTITVDAPADPRYRVAFLASIENIEVEPAETAAKVIVNSRTGTVVVGSNVR
                     VFPAAITHGSLTVTITDDTTVSQPSPLSGGSTVVVPRQNIDVREEKRPMFLFKPGVKL
                     EDIVKAVNQIGASPSDLVAILEALKAAGALRAELLVI"
BASE COUNT          467 a         1063 c         1006 g          426 t
ORIGIN      
        1 tgtactgctg cgcctcggcg aacagggttt cggaacgggt catcgcggga gtctcgctgg
       61 ccggtgaagc gccggcagcc gccggcgggg aagggcgacc ttgtagcgca gccgccggca
      121 aacgcaaacc ccggcatggc cggtgtgtgg gtcgcgatta aaagcccgta agccgcttgc
      181 gaccccggca tgaccccata aggtttgccg caggtccggc cacggccgga cccggccgca
      241 tggacgtcgc aggcagcacc ggggtgcggc gccctgccga agcaccggga aacaccggtt
      301 gccgggtcga tgacttgccg acgggagttg cgccgcacca gcacgcggcg ccggggaaac
      361 agaaagcgcg gatgccggcg atgcccggca tccgccggaa ttcgcgcgac ccgaggacgc
      421 ttccgatgac tccgcaccgc cttgcaccgg ccgccgccgg cctgctgctt tgttccagct
      481 tgcccgccca ggccacgatc gtgctgcaga cgctgaccga tgccgtcgcg gcaccgatgt
      541 acgctgccgc gaccgctgcc gatccggcac acctctatgt cgccgagcgc gacggcaccg
      601 tgcgccgcgt cgagcgcacg agcggtgcgg tcgaggcgac gcccttcctc gacatccgca
      661 gccaggtcaa caccggcggc gagggcgggc tgctcggcat cgccttcgat ccgggctacg
      721 ccggcaacgg gcacttctac gtcaactacc tcgacaactc gcagcagacg cgcgtcgtgc
      781 gctacacggc cgccggtggc gtcgtcgatc cgggctcggc gcagacggtg atcgcgatca
      841 gccagccgca gctcaaccac aacggcggct ggatcggctt cgggccggac aaccagctgt
      901 acgtcgcgac cggcgatggc ggcggttccg acgacaacga cgccggccac acgccgggca
      961 tcggcaacgc ccaggacctg agctccccgc tcggcaagat cctgcgcctc gacgtgggca
     1021 gcgacgcctt cccggccgac ccgcagcgca actacgcgat cccggccgac aacccgctgc
     1081 tcggcggcgc ggccgcacca agcgagatct gggcctacgg cctgcgcaat ccgtttcgcg
     1141 ccggcttcga ccggctgacc ggcgatctgt ggatcggcga tgtcggccag ctcacgcgcg
     1201 aggagatcga cttccagccg gccggcatcg gcggtcagga ctacggctgg cggctgcgcg
     1261 agggcacgat cgcgacgccg tcgggcggcg tcggcgggcc gaatccggat gccgtcgatc
     1321 cggtgcacga gtacgaccac acggtcgggc gctcggtgat cggcggcacc gtctaccgcg
     1381 gctcgatcgc cgaactctac ggccgctact tcttcgccga cttcaacgcc ggccggctgt
     1441 ggacgctcga cggcagcggc gtcaccgact ggacggacct gctgacggcg atcggggcgc
     1501 cgctgctcgg catcgtcggc ttctcggagg atgtcgacgg cgagctgtac ctgatggatc
     1561 tcgggggcgc gatctaccgc atcgccgaga ttccggcacc gggtggcctg gcgctgctgc
     1621 tggccgggct cgggctgctg cggctgcggg cggcacggcc tccggtgctg ggctgaagcc
     1681 tccgtgcgtc cggcgccatc cggtcccgcg tcggccgcac ggcgccggaa atccggcgga
     1741 agttcatcgt catccataaa aaataaataa aatcaatgta ttgatctatg gcctgaatcc
     1801 tgcttgaagc ggcggcatcc acgccgtttc gcaggattcc cgtcatgacc ccagccccga
     1861 ccctgttccg tacgctgctg ctcgccgccc tcgtcggcgt atccggcagc gcggtcgccg
     1921 accgcgtcaa ggatctggcg agcgtcggcg gcgtgcgcag caaccagctg atcggctacg
     1981 gcctggtcgt cggtctggcc ggtaccggcg accagacctc gcagacgccg ttcacggtgc
     2041 agtcgctgcg caacatgctg accggcctcg gcgtcgtgct gccggccaac atcagcctgc
     2101 agctgaagaa cgtcgccgcg gtgtcggtgc atgccgagct gccgccgttc gcgaaggccg
     2161 gccagaccgt cgacgtgacc gtgtcctcgc tcggcaacgc caagagcctg cgcggcggtt
     2221 cgctgctgct gtcgccgctg cgcggcaccg acggcgaggt ctacgcgatc gcgcagggca
     2281 acgtcgtcgt cggcgggctc ggcgtcgagg gcgccggttc cagcatcacc gtcaacgtgc
     2341 cgagctccgg gcgcatcgcc aacggcgcga ccgtcgagcg cccggtgccg gacggcatga
     2401 cgcacgccga agggccgctg atgctgaacc tgcgccagag cgacttcacg accgcgcagc
     2461 gcgtcgccga cgccatcaac cgcaagctgc acagccaggt cgccgcgccg atggatgcga
     2521 cgacgatcac ggtcgatgcg ccggccgatc cgcgttaccg cgtcgccttc ctcgccagca
     2581 tcgagaacat cgaggtcgag ccggccgaga cggcggcgaa ggtcatcgtc aactcgcgca
     2641 ccggcaccgt cgtcgtcggc agcaacgtgc gcgtgttccc ggccgcgatc acgcacggca
     2701 gcctgaccgt gaccatcacc gatgacacga cggtcagcca gccgtcgccg ctcagcggcg
     2761 gcagcaccgt cgtcgtgccg cgccagaaca tcgacgtgag ggaagagaaa cggccgatgt
     2821 tcctgttcaa gcccggcgtg aagctcgaag acatcgtcaa ggccgtcaac cagatcggtg
     2881 cctcgccgtc ggatctggtc gcgatcctcg aagccctgaa ggccgccggt gcgctgcgcg
     2941 ccgaactgct ggtgatctga ac
//
LOCUS       DAOQJH010000348         2953 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_22746_length_2953_cov_3.597308, whole genome shotgun sequence.
ACCESSION   DAOQJH010000348 DAOQJH010000000
VERSION     DAOQJH010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2953)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2953)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2953
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22746_length_2953_cov_3.597308"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1107)
                     /locus_tag="PLQ18_08480"
     CDS             complement(<1..1107)
                     /locus_tag="PLQ18_08480"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HNE01727.1"
                     /translation="MGRPLPADGFSQAADAPAATVVEVDQVRYEAGLVELLYRQFGAI
                     FFGNALVLVFSVWLLRNYVPTLRLIGWALVVLAVNLTRRALVQAYERAPDRLSRPRRW
                     ARYYTVGAAMSGIVWGSVGLLFFNENNMTVTAYLCIVVAGMAGGSIATYGSWRPAYLM
                     FALPVALPFAARTLWEGGLVYTELGALCLFVLVLNLSFFSKIHATLCETVRLRLENQA
                     LVGELTIQKDNAEAATRVKARLLAAASHDLRQPVHSLGLFLGTLEELGRRPVLQPADV
                     GRIAVSMQRALGALSGLLGALLDLSRLDAGTVEPKPAPLALEDILDTLHSQFAGPARA
                     QGLRFASVPSRLWVETDRGLLLQVLSNLCTNALRY"
     gene            1251..1886
                     /locus_tag="PLQ18_08485"
     CDS             1251..1886
                     /locus_tag="PLQ18_08485"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNE01728.1"
                     /translation="MPTFVIAEDHALVREGLALRLSLEPGWEVIGETGDGSEVEGLVV
                     ERRPDVLILDLQLDGMQGLEVCTAVRRRLPDQLILVLTANPEADVARRALDAGADGYL
                     IKRGDGAELVHAIRTLLDGSGYISDDIGREVVSGGKVDTPVDALSPRERHVLALIAAG
                     HGSREIADQLSISVGTVRKHRENLMQKLDLHSIAEVTSFAIKNGLYTPPRG"
     gene            2578..>2953
                     /locus_tag="PLQ18_08490"
     CDS             2578..>2953
                     /locus_tag="PLQ18_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015927543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNE01729.1"
                     /translation="MNPRPSSRPRGATRDELVHIGVRMLTEKGYGQTGLDEILRTAGV
                     PKGSFYHYFPSKEAYGLAVVEHYAQYFAKRLARAFDDDSLTPLDRLRRFVTEACAGMI
                     RHEFRRGCLVGNLGQELGAHNDA"
BASE COUNT          493 a         1057 c          988 g          415 t
ORIGIN      
        1 gtagcgcagc gcattggtgc agaggttcga cagcacctgc agcagcaggc cgcgatcggt
       61 ctcgacccag aggcggctcg gcacgctggc gaagcgcagg ccctgcgcac gtgccggacc
      121 ggcgaactgg ctgtgcagcg tgtcaaggat gtcctcaagg gcgagcggcg ccggcttcgg
      181 ctcgaccgtg ccggcatcga gccgtgacag gtcgagcagg gccccgagca ggccggacag
      241 cgcaccgagc gcgcgctgca tgctgaccgc gatgcgtccg acatcggccg gctgcagtac
      301 cggccgccgg ccgagttcct ccagcgtgcc gaggaagagg ccgagcgaat gcacgggctg
      361 acgcaggtcg tgactcgccg ccgccagcag ccgtgccttg acgcgcgtcg cggcctcggc
      421 attgtccttc tggatggtca gctccccgac cagcgcctgg ttttcgaggc gcaggcgcac
      481 cgtctcgcac agcgtcgcgt ggatcttcga gaagaacgac aggttcagca ccagcacgaa
      541 caggcacagc gccccaagct cggtgtagac cagcccgccc tcccacagcg tgcgcgccgc
      601 aaacggcagc gccaccggca acgcgaacat caggtaggcc ggccgccacg agccgtaggt
      661 cgcgatcgaa ccgccggcca tgccggcgac gacgatgcag agatacgccg tgacggtcat
      721 gttgttctcg ttgaagaaca gcaggccgac cgagccccag acgatgccgg acattgccgc
      781 gccgacggtg tagtagcgcg cccagcgtcg cggccggctc aggcgatccg gtgcccgctc
      841 gtaggcctgc accagcgccc ggcgcgtcag gttgacggcg agcaccacca gtgcccagcc
      901 gatcagccgc agcgtcggca cgtagttgcg cagcagccag acggaaaaca ccagcaccag
      961 cgcattgccg aagaagatcg ccccgaactg ccggtacagc agctcgacca gcccggcctc
     1021 gtagcggacc tgatcgacct cgacgaccgt ggctgccggt gcatccgcgg cctgggaaaa
     1081 tccgtctgcc ggaagtggtc tgcccattgc ttcatcctgt gctgcatccg gataccgaac
     1141 cgcaggccgc gcgcccggcg gggctgcgac tcgcggtctc cggcacacgc ctgcgcgcct
     1201 gccgtgggtg ggatacgata tttgacgtaa tagcagcaga ggaaacggaa atgccgacgt
     1261 tcgtgatcgc cgaggaccat gcgctggtcc gggaaggatt ggccctgcgc ctgtcgctgg
     1321 agccggggtg ggaggtcatc ggcgagaccg gcgacggcag cgaagtcgag ggtctggtcg
     1381 tcgagcgaag gcccgatgtg ctgatcctcg acctgcagct cgacggcatg cagggcctgg
     1441 aggtctgcac cgcggtgcgc cggcgcctgc ccgatcagct gatcctggtg ctgactgcca
     1501 acccggaggc cgatgtcgca cgccgtgcgc tcgatgccgg tgccgacggc tacctgatca
     1561 agcgcggcga cggcgccgaa ctggtgcatg cgatccgcac gctgctcgat ggcagcggtt
     1621 acatcagcga cgacatcggc cgcgaggtcg tcagcggcgg caaggtcgac accccggtcg
     1681 atgcactgag cccgcgcgaa cgccacgtgc tggcgctgat cgccgccggg cacggcagcc
     1741 gcgaaatcgc cgaccagctg tcgatcagcg tcggcaccgt gcgcaagcac cgcgagaacc
     1801 tgatgcagaa gctcgacctg cacagcatcg ccgaggtcac gagcttcgcg atcaagaacg
     1861 gcctgtacac gccgccgcgc ggctgaattc cctgaatcct ccccgagtcc tcacgcctgc
     1921 gacgcaggcg gaccggatac gacgtttgcc ggattgttcc gccgtcctgc ggcatcgaca
     1981 ctccccccgt gcttcggccc ctgccggagc gcaccgcatc aacccgcaag gtgcagggaa
     2041 gtgcaccgca acccggggtc gccggtacgg aaaccagcgg gcaccggcgg gctggacgcc
     2101 ggccggtcgc tgaagcgggg ctgctgatac gggttgccat cggcacggag gccggcatcg
     2161 gacgggtgag tggccggcgt tgtcgcgggg acggcgccag gccacgggtc agtcacctgc
     2221 gcttccggta cggcggccgt catggccggg gcggcggaac ggttgggcgc aaccattccg
     2281 ccgtctccgg ccggcagcgc agatcgcccg caaccgtccc cccgcaccgc gccccctgcc
     2341 tgatccgcct gccccgcacc gtgccgcgtt ccgcggcggg ctgccggtcc ggtcttcccg
     2401 gcaaagcctt ccgtccgcag gccggcctgc acgcctgcgc tgccggaccg gcctgcacgc
     2461 cggaggcagc acggccgtcc ctccccccgc ctcttcgaaa tcggtatcgc tgccacgcag
     2521 aaggcggccg gaagtgaggc tgtcattctc tagacgaccg gtctaatatc cagtcccatg
     2581 aacccacgcc catcttcccg cccgcgcggc gccacgcgcg acgaactcgt gcacatcggt
     2641 gtgcgcatgc tgaccgagaa gggctacggc cagaccggtc tcgacgagat cctgcgcacc
     2701 gccggcgtgc ccaaaggttc gttctatcac tactttccga gcaaggaggc gtacggactc
     2761 gccgtcgtcg agcactacgc gcagtacttc gcgaagcgcc tcgcgcgcgc cttcgatgac
     2821 gactcgctca ccccgctcga ccggctgcgg cgcttcgtga ccgaagcctg cgccggcatg
     2881 atccggcacg aattccgccg cggctgcctg gtcggcaacc tcggtcagga actcggtgcg
     2941 cacaacgacg cct
//
LOCUS       DAOQJH010000349         2947 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_22825_length_2947_cov_5.359959, whole genome shotgun sequence.
ACCESSION   DAOQJH010000349 DAOQJH010000000
VERSION     DAOQJH010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2947
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22825_length_2947_cov_5.359959"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(94..621)
                     /locus_tag="PLQ18_08495"
     CDS             complement(94..621)
                     /locus_tag="PLQ18_08495"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HNE01730.1"
                     /translation="MSLLPPVTADRFVPAPLFRRLAAAVYDVLPLAGVLVMAEALATG
                     LGRLLLGAEGYGQHAGLAGNPWNTSFVFLVCFAYYGGFWVHGGQTLGLRTWKLRVQLP
                     GGGRIGWWHALLRFLAGLAWLVPGLGLYKALHAPVWMAIALSLACLIATLASRLPDRA
                     AGTEMVRLLRDGGGG"
     gene            738..1091
                     /locus_tag="PLQ18_08500"
     CDS             738..1091
                     /locus_tag="PLQ18_08500"
                     /inference="COORDINATES: protein motif:HMM:NF021460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2173 family protein"
                     /protein_id="HNE01731.1"
                     /translation="MSIVADLMNLPGVIAAGEYAFRGDRYSYRGQLTEDQARMCTILC
                     RTSTMGIVMQGRMAETALPGAGIRPRGWAMRGPEHAMCVVGNVFCVMKNQSGTMDSVL
                     ARLNTAMADVDMHLV"
     gene            1226..1612
                     /locus_tag="PLQ18_08505"
     CDS             1226..1612
                     /locus_tag="PLQ18_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018506659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2173 family protein"
                     /protein_id="HNE01732.1"
                     /translation="MARLGNLLDQPGVLAAFEFRASGELLDHELRAGSPITTDILELL
                     ASMCAANVSICGMQARGWEKLTGMSGFSNVREFTLVGFDWSVVVSCAQREAQRAAGQE
                     TIAPYQGVVLINRDARYEAACKALEA"
     gene            1623..1958
                     /locus_tag="PLQ18_08510"
     CDS             1623..1958
                     /locus_tag="PLQ18_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018952929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2173 family protein"
                     /protein_id="HNE01733.1"
                     /translation="MIKRLLAVDGVLAVCRFRDDGGMVESYGVGLDQDQLGKLSLVAH
                     EYRRMLQGHADLFSMFTNMRGWTPPRGWAVRGEKLTLCGVAGVVCILDNAEADYDEVL
                     KDMIEIAHW"
     gene            2181..>2947
                     /locus_tag="PLQ18_08515"
     CDS             2181..>2947
                     /locus_tag="PLQ18_08515"
                     /inference="COORDINATES: protein motif:HMM:NF040455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE01734.1"
                     /translation="MPSSFHRCLRALLLPAVLLVPISVPAAPAATEPVAAELRTRLAA
                     PEALAGEALRERPLLARFYAARAGKPAWSEPRLLDALLAAVDRSPEDGLDAGDYHRAR
                     LATAIDAARRGRLDATGLAALDVLATDAWLALAQDLLSGRLDPQQLDKDWTLRPRSRD
                     LVAALNEALTNRRIGESLAALAPAYPEYAQLRRGLAELRRQLAAGGWPTVGALAKGEK
                     IEPGQPHPRVREIRARLLATREVEATTPVLAPVSVPAP"
BASE COUNT          445 a         1072 c         1042 g          388 t
ORIGIN      
        1 cggccgggcg tcgatctccc ctctcccctt gcgggagagg ggccggggga gagggtgcgc
       61 atgcctgcgc aggcgttgcg actgcgcgtg cgttcagccg cccccgccat cccgtagcag
      121 ccggaccatc tcggtcccgg ccgcccggtc gggcaggcgg ctcgccagcg tcgcgatcag
      181 gcaggcgagc gacagcgcga tcgccatcca caccggcgcg tgcagtgcct tgtagagccc
      241 cagccccggc acgagccagg cgaggccggc caggaagcgc agcagcgcat gccaccagcc
      301 gatgcgcccg ccgccgggca gctgcacacg cagcttccag gtgcgcaggc cgagcgtctg
      361 gccgccgtgg acccagaagc cgccgtagta cgcgaagcag acgaggaaca cgaagctggt
      421 gttccacggg ttgccggcga gcccggcatg ctgcccgtag ccttcggccc cgagcagcag
      481 ccggccgagc ccggtggcca gcgcctcggc catgaccagc acgccggcga gcggcagcac
      541 gtcgtagacc gcggcggcga ggcggcgaaa cagcggggcg ggaacgaagc ggtcggcggt
      601 gaccggcggc agcagcgaca tggacaggac cggggcagga aaacggcggg gcatttaact
      661 atgttccgcg aggccgccga cagtgcggcc gacggccgca gcgccgtcac acgaccaagc
      721 accaccgggg agcctgaatg agcatcgtgg ccgatctgat gaacctgccc ggcgtcatcg
      781 ccgccggcga gtacgcgttc cgcggcgacc gctattccta tcgcgggcag ctcaccgagg
      841 atcaggcacg catgtgcacg atcctctgcc ggacctcgac gatgggcatc gtcatgcagg
      901 gccgcatggc cgaaaccgcg ctgcccggcg ccggcatccg tccgcgcggc tgggcgatgc
      961 gcggacccga gcacgcgatg tgcgtggtcg ggaatgtctt ctgcgtgatg aagaaccagt
     1021 ccggcacgat ggactcggtg ctcgcgcgcc tgaacacggc gatggccgac gtcgacatgc
     1081 acctcgtctg atccgcgccg gcagcaccac ccgtacgcga cctgccttcc agaagtcatc
     1141 ctgccgtcat ccggcgccat cccgccggcg gcacccgcga ccagaccatc ggcaaagacc
     1201 gacttcgtca ggagagagaa acaacatggc cagactcgga aacctgctcg accagcccgg
     1261 cgtgctcgcc gccttcgagt tccgcgccag cggcgaactg ctcgaccacg agctgcgcgc
     1321 cggttcaccg atcacgaccg acatcctcga actgctggcc agcatgtgcg cggccaacgt
     1381 cagcatctgc ggcatgcagg cgcgcggctg ggagaagctc accggcatga gcggcttctc
     1441 gaacgtgcgc gagttcacgc tggtcggctt cgactggtcg gtggtcgtca gctgcgcgca
     1501 gcgcgaggcc cagcgcgctg ccggccagga gacgatcgcg ccgtaccagg gggtcgtgct
     1561 gatcaaccgc gatgcccgct acgaagcggc ctgcaaggcg ctggaagcct gaggaggccg
     1621 acatgatcaa gcgcctgctc gcagtcgacg gcgtgctcgc cgtctgccgc ttccgcgacg
     1681 acggcggcat ggtcgagagt tacggcgtcg ggctggatca ggaccagctc ggcaagctgt
     1741 cgctggtcgc gcacgaatac cggcgcatgc tgcagggcca cgccgacctg ttctcgatgt
     1801 tcacgaacat gcgcggctgg acgccgccgc gcggctgggc ggtgcgcggc gaaaagctga
     1861 cgctgtgcgg cgtcgccggc gtcgtctgca tcctcgacaa cgccgaagcc gactacgacg
     1921 aggtcctgaa ggacatgatc gagatcgcgc actggtaggc aggccgggcg tgcggggagc
     1981 gcccggcatc ctccgtgcgc gttcgcccgc atccgcccgc gccggcccgg atgcggcaag
     2041 cggcggtcgg agcagacaat ttccggccgc cgcgcgcgac ggcagagggc tggctttgat
     2101 aagctgcgcc ggtggtttcg gcgcgacgat tgttgctgct ctgctacgaa accccgcccg
     2161 ttccgtaccg gagtctgcga atgccgtcgt ctttccatcg ctgtctgcgg gcgctgctgc
     2221 tgcccgccgt gctgctggtc ccgatttccg tccctgccgc cccggcggcg accgaaccgg
     2281 tcgccgccga gctgcgcacc cggctcgccg cacccgaggc gctggccggc gaggcgctgc
     2341 gtgagcgtcc gctgctcgcg cgcttctacg ccgcccgcgc cggcaagccg gcctggagcg
     2401 agccgcgcct gctcgatgcg ctgctcgccg ccgtcgacag atcccccgag gacggcctcg
     2461 atgccggcga ctatcaccgt gcccggctgg cgacggcgat cgacgccgcc cgccgcggca
     2521 ggctcgatgc gaccgggctg gccgcgctcg acgtgctcgc gaccgatgcc tggctcgcgc
     2581 tcgcgcagga cctgctctcc gggcggctcg acccgcagca gctcgacaag gactggacgc
     2641 tgcgcccgcg cagccgcgat ctggtcgcgg cgctgaacga ggcgctcacg aaccggcgca
     2701 tcggcgagtc gctcgcggcg ctggcgccgg cctaccccga atacgcgcag ctgcgccgcg
     2761 gtctcgccga gctgcgccgc cagctcgcgg ccggcggctg gccgacggtc ggtgcgctcg
     2821 cgaagggcga gaagatcgaa cccggccagc cgcacccgcg cgtgcgcgag atccgcgcgc
     2881 gcctgctggc gaccagggaa gtcgaggcga cgacgccggt gctggccccg gtcagcgtgc
     2941 cggctcc
//
LOCUS       DAOQJH010000350         2943 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_22882_length_2943_cov_3.200831, whole genome shotgun sequence.
ACCESSION   DAOQJH010000350 DAOQJH010000000
VERSION     DAOQJH010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2943)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2943)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2943
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22882_length_2943_cov_3.200831"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(229..699)
                     /locus_tag="PLQ18_08520"
     CDS             complement(229..699)
                     /locus_tag="PLQ18_08520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE01735.1"
                     /translation="MTIPAETELTAEARKAISDAFDTIEKNLPFLISLTPDERKGRVL
                     MGDRNKPFVERTLEGAHIAPELLPRNIVIANVERQLALYRALSPVVARMTSLAEQIED
                     THSQVGSEVYTQALEMYRHFKAAGRTEGLDELIDNMGKRFNGQGRKKKTPPKDE"
     gene            969..1280
                     /locus_tag="PLQ18_08525"
     CDS             969..1280
                     /locus_tag="PLQ18_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009515365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HNE01736.1"
                     /translation="MFTVVETSLFQRQWPFYWSEEERGAFAAYIAGHPAAGDVVPESG
                     GIRKVRWSRSGSGKSGGVRVIYYTRIHEGMVVLLTLYAKSKTDNLTGQQLKEIRRVLE
                     G"
     gene            1264..1581
                     /locus_tag="PLQ18_08530"
     CDS             1264..1581
                     /locus_tag="PLQ18_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019562154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNE01737.1"
                     /translation="MSSKGKPLDEAELEAWEATRDLAAELLQSVREMKAGQVHVVSSP
                     VIEARRKTGLSQSQFAALLGVSVRTLQGWEQGRKQPSGAARTLLAIASKNPKALLDVI
                     GEL"
     gene            complement(1594..2142)
                     /locus_tag="PLQ18_08535"
     CDS             complement(1594..2142)
                     /locus_tag="PLQ18_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015478202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNE01738.1"
                     /translation="MPPESLLSADPLCPACPGVRLRIATPADLPLILGYIRALADYER
                     LSDQVVADEALLGVHLFGERPAAEVLLAELDGAPAGFALYFTSFSTFLGRPGIYLEDL
                     WVEPACRRRGVARTLLVALARLALARGCGRFEWSVLDWNEPAIRFYRALGAVGMDEWT
                     VQRVSGAALVRLAGGEIAADAG"
     gene            complement(2177..>2943)
                     /locus_tag="PLQ18_08540"
     CDS             complement(2177..>2943)
                     /locus_tag="PLQ18_08540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE01739.1"
                     /translation="RGAASGRRAAGAPGMKDQMPLKLAARERASFDNYVPGANLAVLE
                     HLRRYTGSPGEPVLYLHGPDGSGRTHLVQAVCSAAAARGQTTVRVGLTDTERLPLAAL
                     DGLENLAVVCIEDLDAIAGQRTWEAALDQLCARLVARGGGLVMTGARVAVAAGFTHPG
                     FAKRVAQAMDFALAPLDAAGFAALLAARAGRLGLALPREVAALLLNHCADHPGRLIAA
                     LDALERALPQARGRLGVTGVRALLRAEGLLAAPPSG"
BASE COUNT          487 a         1010 c         1022 g          424 t
ORIGIN      
        1 ttcaaacata accagtttcg cttcaacgga aaccatgtta gcaatcaccc tatgctgact
       61 tccgaaaata cctagtcagc tcccctccaa aggcaagccg ctgcccgacc acggccagcc
      121 gctcgcctgc gcacaccacc ggcaccggac aaaacaaacc ccgctgcccg tgccggctcg
      181 cgcagtggcg ggccggcaca ggcagcgggg ttcagggatc agaccggatc actcgtcctt
      241 cggcggcgtc ttcttcttgc ggccctgacc gttgaagcgc ttgcccatgt tgtcgatcag
      301 ctcgtccaga ccctcggtgc ggcccgccgc cttgaagtgg cggtacatct ccagcgcctg
      361 cgtgtagacc tcggacccca cctggctgtg cgtatcctcg atctgctcgg ccagactggt
      421 catgcgcgcc accaccggcg acagcgcccg gtacagcgcc agctgccgct cgacattggc
      481 aatcacgatg ttgcgcggca gcagctccgg cgcaatgtgc gcgccctcca gcgtgcgctc
      541 gacgaacggc ttgttgcggt cccccatcaa cacccggccc ttgcgctcgt cgggcgtcag
      601 cgagatcagg aacggcaggt tcttctcgat ggtgtcgaag gcatcgctga tcgccttgcg
      661 cgcctcggcc gtcagttcgg tttcagcggg aatcgtcatc atcatctcct cctcccgtgg
      721 ttggcgccca cgccgcgccg gcaccgccgt cgcgcccctg cgcttcccgt cgcggtcctg
      781 ccacgcgaat acccccgaat ccgccggcgg aaccatcagg cagatgtcgt attgcggatg
      841 acgatttgtt caggcgaggc gggaaagccg gaaagccggt cccgaaactg ccggacagaa
      901 tggactgatg cggtggccca tcgtttgcgc aagtagacga ccctgcgccc gaggcgtagg
      961 gtcgcggcat gttcaccgtc gtcgaaacca gcctctttca gcggcagtgg ccgttttact
     1021 ggtcggaaga ggaacgcggc gctttcgccg cctatatcgc cgggcatcct gctgccggcg
     1081 atgtcgtgcc cgaatccggc ggcatacgca aagtacgctg gagtcgctcg ggctcgggca
     1141 agtccggcgg ggtgcgggtc atttactaca cccgcatcca tgagggcatg gtcgtactgc
     1201 tcaccttgta tgcgaaatca aagaccgaca acctgaccgg ccagcaactg aaggagatcc
     1261 gccgtgtcct cgaagggtaa gccactcgac gaagcagaac tggaagcctg ggaagccacg
     1321 cgggatcttg cggcagaact gctgcagtcc gtgcgtgaaa tgaaggcggg acaggtgcat
     1381 gtcgtgagtt cgccggtcat cgaagcgcgc aggaaaaccg gcttgtcgca atcgcagttc
     1441 gctgcattgc ttggagtgtc cgttcgcacc ctgcagggct gggaacaggg tcgcaagcaa
     1501 ccgagcggtg cagcccgcac cttgctggcg attgccagca aaaaccccaa ggcgctgctg
     1561 gatgtgatcg gcgaactgta agccggcaaa cgctcagccc gcatccgccg caatctcccc
     1621 gcccgccagc cgcaccagtg ccgcgccgct gacgcgctgc accgtccatt cgtccatgcc
     1681 gacggcgccg agcgcgcggt agaagcggat cgccggttcg ttccagtcga gcaccgacca
     1741 ttcgaagcgc ccgcagccgc gcgccagcgc cagccgcgcg agtgcaacca gcagcgtgcg
     1801 ggccacgccg cggcggcggc aggccggttc gacccagaga tcttcgaggt aaatgccggg
     1861 gcggccgagg aaggtcgaga agctggtgaa gtacagcgcg aagccggccg gggcgccgtc
     1921 gagttcggcg agcagcacct cggcggccgg gcgttcgccg aacagatgca cgccgagcag
     1981 cgcctcgtcg gcgaccacct gatcggacag acgctcgtag tcggcgagcg cgcggatgta
     2041 gccgaggatc agcggcaggt cggcaggcgt ggcgatgcgc agccgcacgc cgggacaggc
     2101 cgggcacagc ggatcggcgg acagcaggga ttcgggcggc acgggcaggg gctccacgga
     2161 cggcggggcg gagggttcag ccggacggcg gcgcggcgag caggccctcg gcgcgcagca
     2221 gcgcacgcac gccggtgacg ccgagccggc cgcgggcctg cggcagcgcg cgctccagcg
     2281 catcgagcgc ggcgatcagc cggccggggt gatcggcgca gtgattgagc agcagcgcgg
     2341 cgacctcgcg cggcagcgcc agcccgagcc ggccggcgcg ggcggcgagc agcgcggcga
     2401 agccggcggc atcgagcggg gcgagcgcga agtccatcgc ctgcgcgacg cgcttcgcga
     2461 agcccggatg cgtgaagccg gccgcgaccg cgacgcgggc accggtcatc accagcccgc
     2521 cgccgcgcgc caccagccgc gcgcagagct gatcgagcgc ggcctcccag gtgcgctggc
     2581 cggcgatggc gtcgaggtcc tcgatgcaga ccacggcgag gttttcgagg ccgtcgagcg
     2641 cggcgagcgg caggcgttcg gtgtcggtga ggccgacgcg caccgtcgtc tggccgcggg
     2701 cggcggcggc gctgcacacc gcctgcacca ggtgcgtgcg cccgctgccg tcggggccgt
     2761 gcaggtacag caccggctcg ccgggactgc cggtgtagcg gcgcaggtgt tcgagcacgg
     2821 cgaggttggc gccgggcacg tagttgtcga agctcgcgcg ctcgcgggcg gcgagcttca
     2881 gcggcatctg gtccttcatg ccgggcgctc cggcggcgcg ccggccggac gcagcgcctc
     2941 gat
//
LOCUS       DAOQJH010000351         2938 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_22948_length_2938_cov_3.344433, whole genome shotgun sequence.
ACCESSION   DAOQJH010000351 DAOQJH010000000
VERSION     DAOQJH010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2938)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2938)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2938
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22948_length_2938_cov_3.344433"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1291)
                     /locus_tag="PLQ18_08545"
     CDS             complement(<1..1291)
                     /locus_tag="PLQ18_08545"
                     /inference="COORDINATES: protein motif:HMM:NF017569.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNE01740.1"
                     /translation="MADPGLTQAASTPAAVVAAVETPLAPLSELPARLGTPAIVCAHG
                     ELHARLAVLPQLRAALIRGERPPPGWPHGVLREALFDTLDRLDLPAFCTGQPELADRV
                     LLSLLFHADRTIDEPAGTSEEDAAARAAERFAADWAEMVEEVREWRWVFDALGDVAKA
                     MRWNEARGLLKSAGWQDLVRIRRLLERLEPLSVAIRRLGRALLTDDPDPDSAPVLPQY
                     ESAVEHRRQWREITVPEVAGETCGLERSARVARMLPSEAQLLGHPRLRKLWFARFAER
                     TLLTYRERNRAREHVVVEVPVLKPLPQPLPERRMERGPIVVCVDTSGSMQGAAGEVAK
                     AAVLEAMRVAHAQQRPCYVYAFSGPEDIAERELPLSVDGVEALLDFIAQSFSGGTDIA
                     GPLTRALDRMDDAQWRLADVLIASDGEFGLPRLLVERL"
     gene            complement(1284..2663)
                     /locus_tag="PLQ18_08550"
     CDS             complement(1284..2663)
                     /locus_tag="PLQ18_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNE01741.1"
                     /translation="MSSELYERLRGLLDALSAHLIERDTEIRLSLLAALAGEHLLLLG
                     PPGTAKSELARRLRHALRDARYFERLLTRFSVPEELFGPLSIVALEQDRYERQTAGFL
                     PEASIAFIDEVFKANSAILNALLTLLNEREFDNGSRRLRTPLVTVLAASNEVPEDEAI
                     AAFHDRFLLRCDVQPVGAGRFPELLALPDDIAITVPDACRFDHAELDALRQAAAAVAL
                     PAAVVELIADLRAWTLEQGIAVTDRRWRKLAQLLRIAAHAHGRSQVDLWDCALLVYGV
                     GETAAQRATVAQWYGERLGTDRVLQPARLTRVVESFEAQLEFERTATDLNYDAAGRLA
                     LEAGAEDDDNGLRSSRRKGLSGFIRPKQYGERHIGARVEQVGALLAEFDAALGGLDRI
                     ALELEETVRLHLWLPPDFADRVREPLAVTRAVFAALRARVIDVREGFAALPLADEPGT
                     APAPIAVDG"
BASE COUNT          449 a         1025 c         1057 g          407 t
ORIGIN      
        1 cgaggcgctc gaccagcagc cgcggcaggc cgaattcgcc gtcgctggcg atcagcacat
       61 cggcgagccg ccactgcgca tcgtccatgc gatcgagcgc gcgcgtgagc gggccggcga
      121 tgtcggtgcc gcccgagaag ctctgtgcga tgaaatcgag cagcgcctcg acgccgtcga
      181 cgctcagcgg cagctcgcgc tcggcgatgt cctcggggcc ggagaacgca tagacgtagc
      241 agggccgctg ctgcgcgtgc gcgacgcgca tcgcttcgag caccgcggcc ttggcgacct
      301 cgccggccgc gccctgcatc gaacccgagg tatcgacgca gacgacgatc ggcccgcgct
      361 ccatgcgccg ctcgggcagc ggctgcggca ggggcttgag caccggcacc tcgacgacga
      421 cgtgctcgcg cgcgcggttg cgttcgcggt aggtgagcag cgtgcgttcg gcgaagcgcg
      481 cgaaccagag cttgcgcagc cgcggatggc cgagcagctg cgcttcgctc ggcagcatgc
      541 gcgcgacgcg cgccgatcgt tcgaggccgc aggtttcgcc ggcaacctcg ggcacggtga
      601 tctcgcgcca ctggcgccgg tgttcgacgg ccgattcgta ctgcggcagc accggggcgc
      661 tgtcggggtc ggggtcgtcg gtcagcagcg cacggccgag cctgcggatc gccaccgaca
      721 gcggctccag gcgttcgagc agccggcgga tgcgcaccag gtcctgccag ccggccgatt
      781 tcagcagacc gcgcgcctcg ttccagcgca tcgccttggc gacatcgccg agcgcgtcga
      841 acacccagcg ccactcgcgc acttcctcga ccatctccgc ccagtcggcc gcgaagcgtt
      901 cagcggcccg cgcagcagca tcctcttcgc tggtgccggc cggttcgtcg atggtgcggt
      961 cggcgtgaaa cagcagcgac agcagcacgc gatcggcgag ttccggctgg ccggtgcaga
     1021 aggccggcag atcgagccga tcgagcgtgt cgaacagcgc ctcgcgcagc acgccgtgcg
     1081 gccagccggg cggcggccgt tcgccacgga tcagcgccgc ccgcagttgc ggcagcacgg
     1141 cgaggcgcgc gtgcagctcg ccgtgtgcgc agacgatggc cggcgtgccg aggcgcgccg
     1201 gcagctcgga caagggtgca agcggggtct cgaccgcggc gacgacggcg gccggagtcg
     1261 atgcggcctg cgtcagcccg ggatcagcca tcgacggcga tcggcgccgg ggccgtaccg
     1321 ggctcatcgg cgagcggcag cgcggcgaag ccttcgcgca cgtcgatcac gcgcgcgcgc
     1381 agcgccgcaa acacagcacg ggtcacagcg agcggctcgc ggacgcgatc ggcgaaatcc
     1441 ggcggcagcc acagatgcag ccgcacggtc tcttccagtt cgagcgcgat gcgatccagc
     1501 ccgccgagcg cggcatcgaa ctcggcgagc agcgccccga cctgctcgac gcgcgcgccg
     1561 atgtgacgct cgccgtactg cttgggacgg atgaagccgg acaggccctt gcgccgcgag
     1621 ctgcgcaggc cgttgtcgtc gtcctcggcg ccggcttcga gcgcgaggcg cccggcggcg
     1681 tcgtagttca ggtcggtcgc cgtgcgctcg aattcgagct gcgcctcgaa ggactcgacg
     1741 acgcgcgtca ggcgcgccgg ctgcagcacg cgatcggtgc cgaggcgctc gccgtaccac
     1801 tgcgcgaccg tggcgcgctg cgcggcggtt tcaccgacgc cgtacacgag cagcgcgcag
     1861 tcccacagat cgacctggct gcgcccgtgc gcgtgtgcgg cgatgcgcag caactgcgcg
     1921 agcttgcgcc agcgccggtc ggtgacggcg atgccctgct ccaacgtcca ggcgcgcagg
     1981 tcggcgatca gctcgacgac ggcggccggc agcgccacgg cggccgcagc ctggcgcagc
     2041 gcatcgagct cggcgtgatc gaagcggcag gcatccggca ccgtgatcgc gatgtcatcc
     2101 ggcagcgcca gcagttccgg gaagcgtccg gcgccgaccg gctgcacgtc gcagcgcagc
     2161 aggaagcggt catggaaggc cgcgatcgcc tcatcctcgg gcacctcgtt gctcgcggcg
     2221 agcacggtca ccagcggcgt gcgcaggcgc cggctgccgt tgtcgaactc gcgctcgttc
     2281 agcagcgtca gcagcgcgtt caggatcgca ctgttggcct tgaacacctc gtcgatgaat
     2341 gcgatcgagg cctcgggcag gaagccggcg gtctggcgct cgtagcggtc ctgttcgagc
     2401 gcgacgatcg acagcggccc gaacagttcc tcgggcaccg agaagcgcgt cagcagacgc
     2461 tcgaagtagc gcgcatcgcg cagcgcatgc cgcagccggc gcgcgagttc gctcttggcg
     2521 gtgccgggcg gcccgagcag cagcaggtgc tcgccggcga gcgccgccag cagcgacagc
     2581 cggatctcgg tatcgcgctc gatcagatgg gccgacaggg catcgagcag cccgcgcagc
     2641 cgttcgtaca gctcactgct catgcgagcg acctccggaa gcggaatcct ggcagtctac
     2701 tgccaccggc cctgccatga ggggtccgga agcggcagac cgctgccgtg ttgccttcat
     2761 tccggagaaa ccattacggg acgggatgaa cttcgtttcc cggcaatggc ttgatgctga
     2821 tcgtttccgg agtttcaatt ccggagtttc tggtcagccg aaaagacgaa accccgatcc
     2881 atgcgtggat cggggtatcg gggataggag cctggcggtg acctactttc acatgggg
//
LOCUS       DAOQJH010000352         2936 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_22971_length_2936_cov_4.274557, whole genome shotgun sequence.
ACCESSION   DAOQJH010000352 DAOQJH010000000
VERSION     DAOQJH010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2936)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2936)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2936
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22971_length_2936_cov_4.274557"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(56..946)
                     /locus_tag="PLQ18_08555"
     CDS             complement(56..946)
                     /locus_tag="PLQ18_08555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HNE01742.1"
                     /translation="MSRRGSGGLKGLAGFLGSGEHQFGQSLMDESAAGVARLPVAQLV
                     PGRFQPRRTIDEAWLAELADSVRRLGVIEPVAVRPLGEGRYEILAGEMRWRAAQQAGL
                     VELPVIVHDVDDRTAASMALVENLLRRDLDPIEEAHALARLGSEFGLRNRELAELLSR
                     SESAISRTLGLLALAPGVQALLQARRLDAGHARVLLGLPEADQEALAQAAVDLGLSVR
                     ELEKRRQRLLAGERPALAEPVVPADAELDALERQFTQRLGAGVRLRYSAARQRGRIEI
                     DFRDLDGLRALLARLPEAGG"
     gene            complement(943..2166)
                     /locus_tag="PLQ18_08560"
     CDS             complement(943..2166)
                     /locus_tag="PLQ18_08560"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNE01743.1"
                     /translation="MEAFTMQALRFNFRTLCRNAAERGEQLQARLLDALGARHGAKAL
                     PDFGIERAAQLIGCTANYIRSLEARAEAPQPRLIEAGSLQRRVYNYNDVNHLRTVMGR
                     GVHRPDGAAAARVVFSNLKGGVAKTTMSLHFAQYCAREGYRVLLVDADPQATTTGAFG
                     FIPDLDLGDGDDLSQALLAGPGHLETAIRRTHWAQLDLLPSRLALQYVDWQLSQPAER
                     THAELGAPPVRLTRALAQVEDRYDLIVIDTPPSLGMLSINAIAAASLIVMPIVPHMYD
                     ISSSVQYFRILEQVCELYAREIDVARLAILLTKVDNSTETLNNIAVINGAYGELILSN
                     RMGLTKELQKSASDQLSIYEIDQPRGSRDTYHRAVMMLDAVNSELLLAVRQLWQQQVL
                     VGIEAAEAGHSRVRA"
     gene            2642..>2936
                     /locus_tag="PLQ18_08565"
     CDS             2642..>2936
                     /locus_tag="PLQ18_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020202204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly(3-hydroxybutyrate) depolymerase"
                     /protein_id="HNE01744.1"
                     /translation="MPRRPAIKPLRTPKTRTLAGPASHIGTGLRRLLSGIATPAEAPI
                     VHARAAGRWEEGSWGLGPLAQRRFRLYLPPGATRARPVPVLLLLHGCGQDSASF"
BASE COUNT          433 a         1039 c         1026 g          438 t
ORIGIN      
        1 accgccggcg gatgcgcgct gccgccttcg acttgccacg tggcaagttc cggcttcagc
       61 cgcccgcctc cggcagccgc gccagcagcg cgcgcaggcc gtcgaggtcg cggaagtcga
      121 tctcgatgcg gccgcgctgc cttgccgcgc tgtagcgcag gcgcacgccg gcgccgaggc
      181 gctgcgtgaa ctggcgttcg agcgcatcga gttcggcatc ggcaggcacg accggctcgg
      241 cgagcgccgg ccgttcgccg gcgagcagcc gctgccggcg cttttccagc tcgcgcacgc
      301 tcaggccgag atcgaccgca gcctgcgcca gcgcctcctg atccgcctcc ggcagtccga
      361 gcagcacgcg ggcgtgaccg gcatcgaggc ggcgcgcctg cagcagcgcc tgcacgccgg
      421 gggcgagcgc gagcaggccg agcgtgcgac tgatggcgga ttcggagcgg ctcagcagtt
      481 cggcgagttc gcggttgcgc aggccgaact cgctgccgag gcgcgcgagc gcgtgcgcct
      541 cctcgatcgg atcgaggtcg cggcgcagca ggttctcgac cagcgccatc gacgccgccg
      601 tgcggtcatc gacatcgtgg acgatgaccg gcagctcgac gagccccgcc tgctgcgcgg
      661 cacgccagcg catctcgccg gcgaggatct cgtagcggcc ttcgccgagc gggcgcacgg
      721 cgaccggctc gatgacaccg agccggcgca cgctgtcggc cagttcggcg agccaggctt
      781 cgtcgatcgt gcgccgcggc tggaagcggc cgggcacgag ctgcgcgacc ggcaggcgcg
      841 caacgccggc ggcggactcg tccatcaggc tctgcccgaa ctggtgctcg ccgctgccga
      901 gaaaaccggc caggcccttc agaccgccgc tgccgcgccg gctcatgcgc gcacccggct
      961 gtgcccggcc tcggccgcct cgatgccgac cagcacctgc tgctgccaga gctggcgcac
     1021 cgcgagcagc agttcgctgt tgacggcatc gagcatcatc accgcgcggt ggtaggtgtc
     1081 gcgcgagccg cgcggctggt cgatctcgta gatgctgagc tgatcggacg ccgacttctg
     1141 cagctccttg gtcaggccca tgcggttgct gaggatcagc tcgccgtagg cgccgttgat
     1201 cacggcgatg ttgttcagcg tctcggtgct gttgtcgacc ttggtcagca ggatcgccag
     1261 ccgcgccacg tcgatctcgc gcgcgtacag ctcgcagacc tgttcgagga tgcggaagta
     1321 ctgcaccgac gaggagatgt cgtacatgtg cggcacgatc ggcatcacga tcaggctggc
     1381 cgcggcgatg gcgttgatcg acagcatgcc gagcgacggc ggcgtgtcga tgacgatcag
     1441 gtcgtagcga tcctcgacct gcgcgagcgc gcgcgtcagg cgcaccggcg gcgcaccgag
     1501 ttcggcatgg gtgcgttcgg ccggctgcga cagctgccag tcgacgtact gcagcgccag
     1561 gcgcgagggc agcaggtcga gctgcgccca gtgcgtgcgc cggatcgcgg tttcgaggtg
     1621 cccggggccg gcgagcagcg cctgcgacag gtcatcgccg tcaccgaggt cgagatcggg
     1681 aatgaagccg aaggcgccgg tggtggtcgc ctgcggatcg gcatcgacca gcagcacgcg
     1741 gtagccctcg cgcgcgcagt actgcgcgaa gtgcagcgac atcgtggtct tggcgacgcc
     1801 gcccttgagg ttcgagaaca ccacgcgcgc cgccgcggct ccgtccggcc ggtgcacgcc
     1861 gcggcccatg accgtgcgca ggtggttgac gtcgttgtag ttgtatacgc gccgctgcag
     1921 gctgccggcc tcgatcagcc gcggctgcgg tgcctcggcg cgtgcctcca ggctgcggat
     1981 gtagttggcc gtgcagccga tcagctgcgc ggcgcgctcg atgccgaaat ccggcagcgc
     2041 cttggcaccg tggcgcgccc cgagcgcatc gagcagccgc gcctgcaact gctcgccgcg
     2101 ttcggcggcg tttcggcaca gcgtgcggaa gttgaagcgc agcgcctgca tcgtgaaagc
     2161 ctccatgatc catcggtgat ggccggcatc gtaggaaggc gcgtgcgcag aaatcaacgc
     2221 atcaggcatg aatgatacgc acgagtccgg atcacgcccg gatcaccgat cgcaggcata
     2281 tgtcgcgctg cggcatgaca ggcttttgac gcatgtcaca gaactgccgt ctttgtccac
     2341 agaatctgtg gaaaacagtc gcatcaatac aacgacttga cgtaattctg atacgagcga
     2401 cacgggtttc cgacacaggt cgcatgcctg caagcggcgg gccggcgagc gagggctgat
     2461 ctggcgcagt ccggtgcaag cctccgcttg caccacgcca gaacccgttc tgccggtcag
     2521 cccgatccgc cgcggcttgc cacgtggcaa gtctccacga tccgcaaacg ccgatccccc
     2581 gctgcccccg gcggctcggg taggctgcca acaccccccg atcgccgaat ccgtcgccct
     2641 tatgccgcgt cgtcccgcca tcaaacccct gcgtacgccg aaaacccgca cgctcgccgg
     2701 gcccgccagc catatcggta ccggcctgcg ccggctgctg tccggcatcg ccacaccggc
     2761 cgaggcgccg atcgtccacg cccgcgccgc cgggcgctgg gaggaaggca gctggggcct
     2821 cgggccgctg gcgcagcggc gtttccggct gtacctgccg ccgggggcga cgcgcgcccg
     2881 gccggtgccc gtcctgctgc tgctgcacgg ctgcgggcag gacagcgcca gcttcg
//
LOCUS       DAOQJH010000353         2934 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_22996_length_2934_cov_4.208753, whole genome shotgun sequence.
ACCESSION   DAOQJH010000353 DAOQJH010000000
VERSION     DAOQJH010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2934)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2934)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2934
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22996_length_2934_cov_4.208753"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(181..1332)
                     /gene="dnaJ"
                     /locus_tag="PLQ18_08570"
     CDS             complement(181..1332)
                     /gene="dnaJ"
                     /locus_tag="PLQ18_08570"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007464705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HNE01745.1"
                     /translation="MSKRDLYEVLGVAKNASDDEIKKAYRRLAMKLHPDRNPDDKVAE
                     EKFKEVKFAYESLSDARKRAAYDQFGHAGLEGGPGGGGPGGAGGPNFSDIFGDIFGDI
                     FTGGGGGGRGGRDSAYRGADLRYMLDLTLEEAVFGTTAKIRVPTLVGCQHCNGSGAKK
                     GTKPAQCPTCNGVGQVRMQQGFFSIQQTCPRCHGRGVVIMDPCEHCHGHGRVEEQKTL
                     SVKVPAGVDNGDRIRLSGEGEAGEGGGPAGDLYVQIRVKPHPIFTREENDLFCEVPIS
                     FTMAALGGEMEVPTLDGRVALKIPSETQTGRVFRLRSKGVKSVRGGLQGDLLCKVTVE
                     TPVNLTARQRELLEQFAESMGEGSHEHSPQSSGWFDKVKKFWDEMTGAG"
     gene            complement(1451..>2934)
                     /gene="dnaK"
                     /locus_tag="PLQ18_08575"
     CDS             complement(1451..>2934)
                     /gene="dnaK"
                     /locus_tag="PLQ18_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_820282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HNE01746.1"
                     /translation="FNDSQRQATKDAGRIAGLEVKRIINEPTAAALAFGMDKSGGKDR
                     KIAVYDLGGGTFDISVIEIADVDGEKQFEVLSTNGDTFLGGEDFDKRVIDYLVEQFQK
                     DTGINLASDPLALQRLKESAEKAKIELSSAQQTEINLPYITADASGPKHLNLKLTRAK
                     LESLVEDLIEKTIIPCRTALKDAGVSVGDIDDVLLVGGQTRMPKVQEAVKAFFGKEPR
                     KDVNPDEAVAIGAAIQGGVLGGTVKDVLLLDVTPLSLGIETLGGVMTKLIEKNTTIPT
                     KQTQTFSTADDNQTAVTVHVLQGEREQAHANKSLGKFDLQDIPLAPRGVPQIEVTFNI
                     DANGILHVSAKDKATGKEQSIVIKASSGLNEDEIQRMVKDAEAHAEEDRKFHELVNAR
                     NGADNLIHASEKTLKDLGEKVEPAERAALESAISELRTAAKGDNKDVITRKTEALGEL
                     SSKLATRLYSAEAGAEGAAGAGAKKPAEDGVVDAEFEEVKDRK"
BASE COUNT          389 a          997 c          990 g          558 t
ORIGIN      
        1 gaggcgttct gttgggatcg gtatcctgtg ctggcagtgc tgatggaagg cgtcggccgc
       61 agacagcaga aaagccgggc tgcgcgcccg gcttttcggg tctgacccag cgcggggtct
      121 gcatcggcgg cagccgggaa tcgtcccgca ccgccctgcc cgcgccggca ctgcagcgga
      181 tcagcccgcg ccggtcatct cgtcccagaa cttcttgacc ttgtcgaacc agccgctcga
      241 ctgcggcgag tgctcgtggc tgccctcgcc catgctctcg gcgaactgtt cgagcagctc
      301 gcgctggcgg gcggtcaggt tgaccggggt ttcgaccgtg accttgcaga gcaggtcgcc
      361 ctgcaggccg ccgcgcaccg acttcacgcc cttgctgcgc agccggaaca cgcgcccggt
      421 ctgggtttcg gagggaatct tcagcgccac gcgaccgtcg agcgtcggga cttccatctc
      481 gccgccgagg gccgccatcg tgaagctgat cggcacctcg cagaacaggt cgttctcctc
      541 gcgcgtgaag atcgggtgcg gcttgacgcg gatctgcacg tacaggtcgc cggccggtcc
      601 gccgccctcg ccggcctcgc cctcgccgga caggcggatg cggtcgccgt tgtcgacgcc
      661 ggccggcacc ttcaccgaca gcgtcttctg ctcctcgacg cggccgtggc cgtggcagtg
      721 ctcgcagggg tccatgatca cgacgccgcg gccgtggcag cgcgggcagg tctgctggat
      781 cgagaagaag ccctgctgca tgcgcacctg accgacgccg ttgcaggtcg ggcactgcgc
      841 gggcttggtg cccttcttcg cgccgctgcc gttgcagtgc tggcagccga ccagcgtcgg
      901 cacgcggatc ttggccgtgg tgccgaacac ggcctcttcg agcgtcaggt ccagcatgta
      961 gcgcaggtcg gcgccgcggt aggccgagtc gcgcccgccg cgtccgccgc cgccaccacc
     1021 ggtgaagatg tcgccgaaga tgtcgccgaa gatgtccgag aagttcggtc cgccggcccc
     1081 acccggaccg ccgccgccgg ggccgccttc gaggccggcg tggccgaact ggtcgtaggc
     1141 cgcgcgcttg cgcgcatcgg acagcgactc gtaggcgaac ttgacctcct tgaatttctc
     1201 ctcggcgacc ttgtcgtcgg ggttgcgatc cggatgcagc ttcatcgcca ggcggcgata
     1261 ggccttcttg atctcgtcgt cgctggcgtt cttggcgacg ccgagcactt catacaaatc
     1321 acgcttggac atcggcacga tccgtcgggg cggcgggcgg gtgcggaacc cgcctcaggg
     1381 acacgacaaa agccgggcgc cggggaaagt cccgacgccc ggccacacac gcacggctca
     1441 accggcgagc ttacttgcga tccttcactt cttcgaactc ggcatcgacg acgccgtcct
     1501 cggccggctt cttcgcaccg gcaccggcgg cgccttcggc tccggcctcg gccgagtaca
     1561 gccgggtcgc gagcttcgag gacagctcgc cgagcgcctc ggtcttcctg gtgatcacgt
     1621 ccttgttgtc acccttggcc gcggtgcgca actccgagat tgccgattcg agcgcggcgc
     1681 gctcggccgg ctcgaccttc tcgccgaggt ccttcagcgt cttttccgag gcgtggatca
     1741 ggttgtcggc gccgttacgg gcattgacca gctcgtggaa cttgcggtcc tcctcggcgt
     1801 gcgcctcggc gtccttgacc atgcgctgga tctcgtcctc gttgaggccc gagctcgcct
     1861 tgatgacgat gctctgctcc ttgccggtgg ccttgtcctt tgccgagacg tgcaggatgc
     1921 cgttggcgtc gatgttgaac gtcacctcga tctgcggcac gccgcgcggg gccagcggga
     1981 tgtcctgcag gtcgaacttg ccgagcgact tgttggcgtg cgcctgctcg cgctcgccct
     2041 gcagcacgtg cacggtgacg gccgtctggt tgtcgtcggc ggtcgagaag gtctgcgtct
     2101 gcttggtcgg gatggtggtg ttcttctcga tcagcttggt catgacgccg ccgagcgtct
     2161 cgatgccgag gctgagcggg gtcacgtcga gcagcagcac atccttgacc gtgccaccga
     2221 gcacgccgcc ctggatcgcg gcgccgatgg cgaccgcctc gtcggggttc acgtccttgc
     2281 gcggctcctt gccgaagaag gccttgaccg cctcctgcac cttcggcatg cgggtctggc
     2341 cgccgaccag cagcacgtcg tcgatgtcgc cgaccgagac accggcatcc ttgagcgccg
     2401 tgcggcacgg gatgatggtc ttctcgatca ggtcctcgac cagcgattcg agcttggcgc
     2461 gcgtcagctt caggttcagg tgcttcggac ccgaggcgtc ggcggtgatg tacggcaggt
     2521 tgatctcggt ctgctgcgcc gaggacagct cgatcttggc cttctcggcc gattccttca
     2581 ggcgctgcag cgccagcggg tcactggcga ggttgatgcc ggtgtccttc tggaactgct
     2641 cgacgaggta gtcgatgaca cgcttgtcga agtcctcgcc gccgaggaag gtgtcgccgt
     2701 tggtcgagag cacctcgaac tgcttctcgc cgtcgacatc ggcgatctcg atgaccgaga
     2761 tgtcgaaggt gccgccgccg aggtcataga ccgcgatctt gcggtccttg ccgccggact
     2821 tgtccatgcc gaaggcgagc gccgccgcgg tcggctcgtt gatgatgcgc ttgacctcca
     2881 gaccggcgat gcggccggcg tccttggtcg cctggcgctg cgagtcgttg aagt
//
LOCUS       DAOQJH010000354         2932 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_23017_length_2932_cov_3.673271, whole genome shotgun sequence.
ACCESSION   DAOQJH010000354 DAOQJH010000000
VERSION     DAOQJH010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2932)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2932)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2932
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23017_length_2932_cov_3.673271"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="PLQ18_08580"
     CDS             <1..189
                     /locus_tag="PLQ18_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013702940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HNE01747.1"
                     /translation="YMYLVSRVRPALATSYAYVNPVVAVLLGVSFGGESISAGGYVAM
                     ALVLAAVVLITRAGERQA"
     gene            complement(245..1651)
                     /locus_tag="PLQ18_08585"
     CDS             complement(245..1651)
                     /locus_tag="PLQ18_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HNE01748.1"
                     /translation="MYNANALPPDLLDELRALLGDRLSTGLAVREHHGRDESSHPAAL
                     PDAVVWPETTEEVSTIARLCHAFRVPMIPFGVGSSLEGHVIPLHGGVSIDLSHMNRIL
                     ALHVEDLDATVQAGVTRKQLNAWLHDSGLFFPIDPGADATLGGMTATRASGTNAVRYG
                     SMREAVLALTVVLADGRVIHTGTRARKSAAGYDLTRLFVGSEGTLGIVTEITVRLHPH
                     LEAISAAVCGFPDVASAARTVIQTIQTGVPVARAELLDALSMRAINAHSKTNWPEVPT
                     LFFEFHGTEAGVAEQAETVQALAHEHGGTDFRWATRQEERTQLWAARHDAYFACLQLR
                     PGSRSLTTDVCVPISRLAECIEATAADVAGMPMPVPLLGHVGDGNFHLLVLTDPDSAA
                     EREAAHALSHRLVLRALAMGGTCTGEHGVGFGKMGYLAEEHGEALDVMRAIKHALDPY
                     GLMNPGKIVETEPQDARR"
     gene            1962..2696
                     /locus_tag="PLQ18_08590"
     CDS             1962..2696
                     /locus_tag="PLQ18_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HNE01749.1"
                     /translation="MPELEGIGGWLFAVLQIIAIDILLGGDNAVVIALACRRLPEDKR
                     RLGVRWGVAGAIVLRIVMIAFALQLLLVPGLKLIGAALLLWIGIKLLQPEADDGHDGI
                     EGGATLLAAVRTVIVADAVMSIDNVVAVAAAARGELALVVFGILVSVPIIVWGSHFVL
                     RLMDRFPVIIVIGGALLGWIAGGLAVHDPLLERLGFDAPDWAHWPASLVGALAVVLIG
                     RQLAARAGRAAPAPLEDLLSEARRDE"
     gene            2741..>2932
                     /locus_tag="PLQ18_08595"
     CDS             2741..>2932
                     /locus_tag="PLQ18_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008525671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNE01750.1"
                     /translation="MSVSKILVPVDGTPESADAVAHALAIVAERGGELHLLHVRLPVE
                     SCHAKLLVDGRTLHDWHREE"
BASE COUNT          393 a         1001 c         1080 g          458 t
ORIGIN      
        1 tacatgtacc tggtcagccg cgtgcgcccg gcgctggcca ccagctacgc ctacgtgaac
       61 ccggtcgtcg cggtgctgct cggcgtcagc ttcggcggcg aatcgatcag cgccggcggt
      121 tatgtggcga tggcgctggt gctggccgcg gtcgtgctga tcacgcgggc cggcgagcgg
      181 caggcatgaa tgccgcggcc gggatggggc ggggcggccg gaggcttcgc aagtcgccgc
      241 gccgtcagcg ccgtgcgtcc tgcggctcgg tctcgacgat cttgcccgga ttcatcagcc
      301 cgtagggatc gagcgcgtgc ttgatcgcgc gcatcacgtc gagcgcctcg ccgtgttcct
      361 cggcgaggta gcccatcttg ccgaagccga cgccgtgctc gccggtgcag gtgccgccca
      421 tggccagcgc gcgcagcacc aggcggtgtg acagcgcgtg cgcggcctcg cgctcggcgg
      481 cgctgtcggg gtcggtcagc accagcaggt ggaagttgcc gtcaccgacg tggccgagca
      541 gcggcaccgg catcggcatg ccggcgacat cggcggccgt ggcctcgatg cattcggcca
      601 gccgcgagat cggcacgcag acatcggtgg tcagcgagcg cgaaccgggc cgaagctgca
      661 ggcaggcgaa gtaggcatcg tggcgcgccg cccagagctg cgtgcgttct tcctgacgcg
      721 tggcccagcg gaagtcggtg ccgccgtgct cgtgcgccag cgcctgcacg gtttcggcct
      781 gttcggcgac gccggcttcg gtgccgtgga actcgaagaa cagcgtcggc acctcgggcc
      841 agttcgtctt cgaatgggcg ttgatcgcgc gcatcgacag cgcatcgagc agctcggcgc
      901 gcgccaccgg cacgccggtc tggatggtct ggatcaccgt gcgcgccgcc gaggccacgt
      961 ccgggaagcc gcagaccgca gccgagatcg cctccagatg cgggtgcagg cgcacggtga
     1021 tctcggtgac gatgccgagc gtgccttcgg aaccgacgaa cagccgcgtc aggtcgtagc
     1081 cggccgccga tttgcgcgcg cgcgtgccgg tgtggatcac gcgcccgtcg gcgagcacga
     1141 cggtcagcgc cagcaccgcc tcgcgcatcg agccgtagcg cacggcgttg gtgccggagg
     1201 cgcgcgtcgc ggtcatgccg ccgagggtcg catcggcacc cggatcgatc gggaagaaca
     1261 gcccggaatc gtgcagccag gcgttgagct gcttgcgcgt gacgccggcc tgcacggtgg
     1321 cgtcgagatc ctcgacgtgc agcgccagga tgcggttcat gtgcgacagg tcgatgctga
     1381 cgccgccgtg cagcgggatc acgtggcctt cgagcgagct gccgacgccg aacgggatca
     1441 tcggcacgcg gaaggcatgg cagagccgcg cgatggtcga gacttcctcg gtggtttcgg
     1501 gccagaccac ggcatcgggc agcgccgccg gatggctcga ttcatcgcgg ccgtgatgct
     1561 cgcgcaccgc cagcccggtc gagaggcgat cgccgagcag cgcacgcagt tcatcgagca
     1621 gatcgggtgg cagggcgttg gcgttgtaca tggcggcagg ctcggggcgc gcggatcggg
     1681 cgaccggcta gtctacgcgg ccctgaaacc gtcgggcagg gtcgcacgta tcggtgaccg
     1741 gcatcgggcg tctgaatctg ctgaagcccg ggacgcctgc cctcaacgct tgcggcacgg
     1801 tcgtgccggg gcatctggtg cgtccggcgt ttgttccgga ccgcccgcgc gttcacaatc
     1861 gcgcgcttta cagctcaggc accgtgcgcc ggtgctgccg gagccgtacc gaatgtcatc
     1921 catccatatg cttgacgcca cgcgcgtcgg ggagacgcgc catgcctgaa ctcgaaggca
     1981 tcggcggctg gctgttcgcc gtgctgcaga tcatcgccat cgacatcctg ctcggcggcg
     2041 acaacgccgt ggtcatcgcg ctcgcctgcc ggcgcctgcc cgaggacaag cgccggctcg
     2101 gcgtgcgctg gggcgtggcc ggcgccatcg tgctgcgcat cgtcatgatc gcgttcgcgc
     2161 tgcagctgct gctggtgccg ggcctgaagc tgatcggggc ggcgctgctg ctgtggatcg
     2221 gcatcaagct gctgcagccc gaggccgacg acggtcacga cggcatcgag ggcggcgcga
     2281 cgctgctcgc cgcggtgcgc accgtcatcg tcgccgatgc ggtcatgagc atcgacaacg
     2341 tcgtcgccgt cgccgctgcg gcgcgcggcg agctggcgct ggtggtgttc ggcatccttg
     2401 tcagcgtgcc gatcatcgtc tggggcagcc acttcgtgct gcggctgatg gaccgcttcc
     2461 cggtcatcat cgtgatcggc ggggcgctgc tcggctggat cgccggcggg ctggcggtcc
     2521 acgatccgct gctcgaacgc ctcgggttcg acgcgccgga ctgggcacac tggccggcca
     2581 gcctcgtcgg ggcgctcgcc gtcgtgctga tcggccgcca gctcgcggcg cgcgccggac
     2641 gcgccgcgcc ggctccgctc gaagacctgc tgtccgaagc gcgccgcgac gaatgacgcc
     2701 cgccgcaccc gatcgcctgc tgcccccggg agttgccgac atgagcgttt cgaagatcct
     2761 ggtgccggtg gacggcacgc ccgaaagcgc cgatgcggtc gcgcacgcgc ttgcgatcgt
     2821 cgcggagcgc ggcggcgagc tgcatctgct gcacgtgcgc ctgccggtcg agtcctgcca
     2881 cgcgaagctg ctcgtcgacg gccgcaccct gcatgactgg caccgcgagg ag
//
LOCUS       DAOQJH010000355         2931 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_23028_length_2931_cov_3.985049, whole genome shotgun sequence.
ACCESSION   DAOQJH010000355 DAOQJH010000000
VERSION     DAOQJH010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2931)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2931)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2931
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23028_length_2931_cov_3.985049"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..773
                     /locus_tag="PLQ18_08600"
     CDS             <1..773
                     /locus_tag="PLQ18_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012352059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNE01751.1"
                     /translation="LDVAANVGFGLKGSKPLARTEREHRVATLLDLVGLKDAGRRYPH
                     ELSGGQQQRVALARALAPQPQLLLLDEPFSSLDASLRERLALDVRTLLKALGTTAILV
                     THDQLEAFALADRIGVMHGGRLRQWATPWQVYHRPADRLVAGFVGQGVWLRGERIDDR
                     HVQLPDLGPVPVPDAPAARWLDVLLRPDDFVRDTASPLRAEVVGRAFRGAECLYTLRL
                     PGGAEVLALLPSQHDHAPGERIGLRLDVDSVTGFAAGG"
     gene            949..1422
                     /locus_tag="PLQ18_08605"
     CDS             949..1422
                     /locus_tag="PLQ18_08605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE01752.1"
                     /translation="MDNHHLAVSLPADRLTRVLNLLRKLEAELPFLIMLSDEERDAMP
                     ILDERKLAYIDRALHLVEAHPDFMPRDFEADEFGTDVQLVKALGLIHSHLLPLARKLD
                     DSLRCAENDACRAALEVHRHASLEDPADTDSLDAMIDSLSPRRFRRCHVSNPAHP"
     gene            1512..>2931
                     /locus_tag="PLQ18_08610"
     CDS             1512..>2931
                     /locus_tag="PLQ18_08610"
                     /inference="COORDINATES: protein motif:HMM:NF016169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HNE01753.1"
                     /translation="MLHLYHSNRMEILAAQLAARLGEPVDDGWAPETLVVASNGMRRW
                     LCLDLADRLGICANLHFPLPSRLFWDIYRAVLPGVPTESAFATPILAWRVLAEFGGLD
                     DDPVWEPLRDYLAGSDDYGRYELARRIADTFDQYLVYRPKWIKNWEKGGAAPGGKGRE
                     DDEDDEDGDDCEGGWQAALWRRLSEVDATHRARLHRQFLKALKQLHERPAGIPARLSF
                     IGIPALPAAWLQTLLGLARWCEVDLYLLNPCAQFWGMIESERTIAKIAPEADHAALYL
                     ETGNRLLASMGRLGRDFHHLLADSTSDLPAAEAFEEPEADTLLAVVQRDLLRLENRGA
                     NHAPSEHDGFTLWAEPYLAAADDHSIQMHVCHSATREIEVLHERLLALFAADDSLAPT
                     DVVVMTPDIEAYAPAVEAVFGAAESGLFIPYGIADRGVRAESPLLDAFLALLELPGGR
                     WGAETVASLLDAAALRRRFGILE"
BASE COUNT          412 a         1056 c          982 g          481 t
ORIGIN      
        1 atctggacgt cgccgccaac gtcggcttcg gcctgaaggg ttcgaagccg ctggcgcgca
       61 ccgaacgcga gcaccgggtc gcgacgctgc tcgatctggt cggcctcaag gacgccggcc
      121 ggcgctatcc gcacgagctt tccggcggtc agcagcagcg tgtggcactg gcgcgcgcgc
      181 tggcaccgca gccgcagctg ctgctgctcg acgagccctt ctccagcctc gacgccagcc
      241 tgcgcgagcg gctggcgctc gatgtgcgca cgctgctcaa ggcactcggc acgacggcga
      301 tcctggtcac gcacgaccag ctcgaagcct tcgcgctcgc cgaccgcatc ggcgtcatgc
      361 acggcgggcg gctgcgccag tgggcgacgc cgtggcaggt ctatcaccgg ccggccgatc
      421 gcctcgtcgc cggcttcgtc ggtcagggcg tgtggctgcg cggcgagcgc atcgatgacc
      481 gccacgtgca gctgcccgac ctcgggccgg tgccggtgcc ggatgcgccg gcggcaagat
      541 ggctggacgt gctgctgcgc cccgatgact tcgtgcgcga cacggcctcg ccgctgcgcg
      601 ccgaggtcgt cgggcgggcg ttccgcggtg ccgagtgcct gtacaccttg cggctgcccg
      661 gtggcgccga ggtgctggcg ctgctgcctt cgcagcacga tcacgctccg ggcgagcgca
      721 tcggcctgcg cctcgatgtc gactccgtga ccggatttgc cgccggcggc tagcgatccg
      781 gcacatctgc gactatttcg tcatgtctga tgcggtggct acggcatctg tcgaagtgac
      841 tgccctctga cctctgccaa tactccgtcc gcgcgctgcc gtgccatgcc gtgctgttac
      901 cgtccccgac ctgctgccca tgtccccggt actgaaggaa ggaacgagat ggacaaccac
      961 caccttgccg tgagcctgcc tgccgatcgc ctgacccgtg tgctgaacct gctgcgcaag
     1021 ctcgaagccg aactgccgtt cctgatcatg ctgtccgacg aggagcgcga cgcgatgccg
     1081 atcctcgacg agcgcaagct ggcctacatc gaccgggcgc tgcacctcgt ggaggcgcat
     1141 ccggacttca tgccgcgcga cttcgaggcc gacgaattcg gcaccgacgt gcagctggtc
     1201 aaggcgctcg gcctgattca cagccatctg ctgccgctgg cgcgcaagct cgatgattcg
     1261 ctgcgctgcg ccgagaacga cgcctgccgc gccgccctcg aagtccatcg ccacgccagc
     1321 ctcgaggacc cggccgatac cgacagcctc gatgcgatga tcgacagcct gagtccgcgc
     1381 cgcttccgcc gctgccacgt gagcaacccg gcgcatccct gaacggctga cctgaacggc
     1441 tgacggaggg ccggcgcctg cgtgccatac tccggcgccc gccggcctcc cgtcgtctcc
     1501 gacccccgcg catgctgcac ctgtaccact ccaaccgcat ggaaatcctg gccgcgcagc
     1561 ttgccgcgcg gctcggtgag ccggtcgatg acggctgggc accggagacg ctggtggtcg
     1621 cctcgaacgg catgcgccgc tggctgtgtc tcgatctcgc cgaccggctc ggcatctgcg
     1681 ccaacctgca cttcccgctg ccgtcgcggc tgttctggga catctatcgc gccgtgctgc
     1741 ccggcgtgcc caccgaatcg gcgttcgcga ccccgatcct cgcctggcgc gtgctcgccg
     1801 agttcggcgg tctcgatgac gatccggtct gggagccgct gcgggactat ctcgccggca
     1861 gcgacgacta cggccgctac gagctcgcgc ggcgcatcgc cgacaccttc gaccagtacc
     1921 tggtctatcg gcccaaatgg atcaagaact gggagaaagg tggcgcggcc ccgggcggca
     1981 agggcaggga ggacgatgag gacgatgagg acggtgatga ctgtgagggc ggctggcagg
     2041 cggccttgtg gcggcgcttg tcggaggtcg atgccacgca ccgcgcacgc ctgcaccggc
     2101 agttcctcaa agccctgaag caattgcacg agcgtccggc cggcatcccg gcgcggctgt
     2161 ccttcatcgg catcccggcg ctgccggcgg cctggctgca gacgctgctc gggctggcgc
     2221 gctggtgcga ggtcgacctg tacctgctca atccctgcgc gcagttctgg ggcatgatcg
     2281 agtccgagcg cacgatcgcg aagatcgcac ccgaggccga tcacgcggcg ctgtacctcg
     2341 aaaccggcaa tcgcctgctt gcgtcgatgg gccggctcgg ccgcgatttc catcatctgc
     2401 tcgccgattc caccagtgac ctgccggccg cagaggcttt cgaggagccc gaggccgata
     2461 cgctgctcgc cgtcgtgcag cgtgacctgc tgcggctcga aaaccgcggt gccaaccacg
     2521 cgccctcgga gcacgacggg ttcacgctgt gggccgagcc ctacctcgcg gcagccgacg
     2581 accactcgat ccagatgcac gtctgccact cggcgacgcg cgagatcgag gtgctgcacg
     2641 agcggctgct ggcgctgttc gcggccgatg attcgctcgc gccgaccgat gtcgtggtca
     2701 tgaccccgga catcgaggcc tatgcaccgg cggtcgaggc ggtgttcggt gccgccgagt
     2761 ccggcctgtt catcccctac ggcatcgccg atcgcggcgt gcgcgccgaa agtccgctgc
     2821 tcgatgcctt tctggcgctg ctcgaactgc cgggcggacg ctggggcgcc gagacggtcg
     2881 cgagcctgct cgatgccgcg gcgctgcgcc ggcgcttcgg catccttgag g
//
LOCUS       DAOQJH010000356         2930 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_23040_length_2930_cov_4.209391, whole genome shotgun sequence.
ACCESSION   DAOQJH010000356 DAOQJH010000000
VERSION     DAOQJH010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2930)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2930)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2930
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23040_length_2930_cov_4.209391"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            327..1052
                     /locus_tag="PLQ18_08615"
     CDS             327..1052
                     /locus_tag="PLQ18_08615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE01754.1"
                     /translation="MNPVITANEVELEFDDDRTARDETRVRSVRSRVRTRKRNHRWSL
                     RSRALFAALVASLMLTVFISLVLSLRMLSLSEENDELRHQNELRDAELTQVRPELERL
                     RADVTELIAKRLPGLRQLVLDQVVPIDDYYLRNVMFTLVKRGKRAGYEYHLTLSNDTL
                     QMVLPRVNIYLFDRHGVHIGSARIGGTGEHVEEVPDLGPGETVTKVGLVTIGEGEEEP
                     VFFRVRVAEPYTAEERESPNPQR"
     gene            complement(1087..2010)
                     /gene="rarD"
                     /locus_tag="PLQ18_08620"
     CDS             complement(1087..2010)
                     /gene="rarD"
                     /locus_tag="PLQ18_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020887847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HNE01755.1"
                     /translation="MSASSETVASPAPGLHPEFATGALYAVLAYLTWGLMPAYWKLVP
                     ELSAPELIAHRVVWSVFVTGALVLAFRRGRELRATLTDTRRLGVLLVTALLVSVNWLV
                     FVWAVLNGHVLESSLGYFINPLFNVLLGVLLLKERLRPLQGLAVALAAAGVLNQGWQL
                     QALPWISLALAASFGAYGLIRKLAPIEPLVGLTVETLLLAPLALAGLLWLDAQGLGHF
                     GHGTPARDLIIIGAGLITALPLLWFANAAKRLRFSTLGFFQYLAPSVQLMLAVLAYGE
                     AFTAVHAVTFGCIWAALALYTWDGRRALRIA"
     gene            complement(2282..2815)
                     /locus_tag="PLQ18_08625"
     CDS             complement(2282..2815)
                     /locus_tag="PLQ18_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003224062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE01756.1"
                     /translation="MTETEFLNLVHQGAGRGSFEEGWESGGAWEIHAQVVIAAFLRSG
                     YGITDARELAYPGSQEHCDFGFTHKGRKYAVELKVENKKDGKFGGMSLDQAMLTDVNK
                     LHAFNADELWFLVIARSDNAKQRLLELAMRGDSWIVDQEAGFLAALCNIRTQPHGLPW
                     QRYEKQALKLEQSPFYQ"
BASE COUNT          517 a          970 c         1010 g          433 t
ORIGIN      
        1 ggcttcgggc agcggcgccg gggggctctc cggggtgtac ttgcagagcg ctcgggtcgg
       61 catggatcat gcgcgccgcc gcggcgtgcc gtatccacgt gaaacgatgt tcatcatcga
      121 ttcctcagcg ttgcgtccat catgccgggg tactgccgca gagcggtttg cgggccaggg
      181 acggacccgt gacgggggga ggaccgatgc actggttgca acgaatcgac gacgacatcc
      241 ggcgacagcg atcgctgcgc gatgacgatg atgaacatga accgtcggcc acggaccgcg
      301 ggcgggatac gggggagaaa gacaacgtga atccggtcat cacggccaac gaagtcgaac
      361 tggagttcga tgacgatcgc acggcacgcg acgaaacccg ggtgcgctcg gtgcgcagcc
      421 gcgtgcgcac ccgcaagcgc aaccaccgct ggagcctgcg cagccgcgcc ctgttcgcgg
      481 cgctggtcgc gagcctgatg ctgacggtgt tcatcagcct cgtgctgtcg ctgcgcatgc
      541 tgtcgctgtc cgaggaaaac gacgagctgc gccaccagaa cgagctgcgc gatgccgagc
      601 tcacgcaggt gcggcccgaa ctcgaacgcc tgcgcgccga tgtcaccgaa ctgatcgcca
      661 agcgcctgcc gggcctgcgc cagctcgtgc tcgatcaggt cgtgccgatc gacgactact
      721 acctgcgcaa cgtcatgttc acgctggtca agcgcggcaa gcgcgccggc tacgagtacc
      781 acctgacgct cagcaacgac acgctgcaga tggtgctgcc gcgcgtgaac atctacctgt
      841 tcgaccgcca cggcgtgcac atcggctcgg cgcgcatcgg cggcaccggc gagcacgtcg
      901 aggaagtccc ggatctcggg ccgggcgaga ccgtcaccaa ggtcgggctc gtgaccatcg
      961 gcgagggcga ggaggagccg gtgttcttcc gcgtgcgcgt cgccgaaccg tacacggccg
     1021 aagagcgcga atcgccgaac ccgcagcgct gagcccggcg cggcaaggcg ctgcgcgtcg
     1081 gccggttcag gcgatgcgca gcgcccggcg gccgtcccag gtgtacagcg ccagcgcggc
     1141 ccagatgcag ccgaaggtca cggcatgcac ggccgtgaag gcctcgccgt aggcgagcac
     1201 ggcgagcatc agctgcacgc tcggcgccag atactggaag aagccgagcg tcgaaaaccg
     1261 caggcgcttc gcggcgttcg cgaaccacag cagcggcagc gccgtgatca gcccggcacc
     1321 gatgatgatc aggtcgcgtg ccggcgtgcc gtggccgaag tggccgaggc cctgcgcatc
     1381 gagccacagc aggccggcga gggcgagcgg cgcgagcagc agggtttcga ccgtcaagcc
     1441 caccagcggc tcgatcggcg cgagcttgcg gatcagcccg taggcaccga agctcgccgc
     1501 cagcgccagc gagatccacg gcagtgcctg cagctgccag ccctggttca gcaccccggc
     1561 cgcggcgagc gccaccgcga gcccctgcag cggccgcagc cgctccttca gcagcagcac
     1621 gccgagcagc acgttgaaca gcggattgat gaagtagccg aggctcgatt cgagcacgtg
     1681 cccgttcagc accgcccaca cgaacaccag ccagttgaca ctgaccagca gcgcggtgac
     1741 gagcagcacg ccgagccggc gcgtgtcggt gagcgtcgcg cgcagctccc gcccgcgccg
     1801 gaaggccagc accagcgccc cggtcacgaa caccgaccag acgacgcggt gcgcgatcag
     1861 ctccggcgcc gacagctccg gcaccagctt ccagtaggcc ggcatcagcc cccaggtcag
     1921 gtaggcgagc acggcataca gcgcgccggt ggcaaactcg ggatgcagcc ccggcgcggg
     1981 gctggcgacg gtttcggacg acgcggacat ggcggcgggg ctcggtgcgg aagggcgcgc
     2041 agcatgggcg ggccggccgc ggccggcaag ccacgctccg gcatgagggg cgtgcagcga
     2101 cgtatcggcg ggtttgcggg ccggggagga tcgctgcggc aggaggcgtc acggcggcga
     2161 actcaggacg gcatgtgcgg gagtcgcggg ggaatcgtgc gtggaatatg gagacgatcc
     2221 ggcgattcat ccggtgcgga ttgcggtcgc cggcatgcac catgccggcg accggtgccc
     2281 ctcactggta aaacggcgac tgctccagct tcagcgcctg tttctcatag cgctgccacg
     2341 gcagtccgtg cggctgcgtc cggatgttgc agagcgccgc gagaaagccg gcctcctgat
     2401 cgacgatcca cgaatccccg cgcatggcga gttcgagcag ccgctgcttg gcattgtccg
     2461 aacgggcgat gacgaggaac cagagttcgt cggcgttgaa cgcgtgcagc ttgttcacgt
     2521 cggtcagcat cgcctgatcg agcgacatgc cgccgaactt gccgtccttc ttgttctcga
     2581 ccttcaactc gaccgcgtac ttgcggccct tgtgcgtgaa gccgaaatcg cagtgctcct
     2641 gcgagcccgg atacgccagt tcgcgggcat cggtgatgcc gtagccgctg cgcaggaacg
     2701 cggcgatgac gacctgcgcg tggatttccc aggcaccgcc gctctcccag ccttcctcga
     2761 aactgccgcg gccggcgccc tggtggacga gattcaggaa ttcggtttca gtcatggtcg
     2821 cattctcgtt tgatctgcgc tgagccgatt cattcgccga gtttttccgc gagcactgca
     2881 gtttgcgatg gttgagtttc tgaaaaaatt tcacttaatc gatttgagaa
//
LOCUS       DAOQJH010000357         2928 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_23083_length_2928_cov_2.755656, whole genome shotgun sequence.
ACCESSION   DAOQJH010000357 DAOQJH010000000
VERSION     DAOQJH010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2928)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2928)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2928
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23083_length_2928_cov_2.755656"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="PLQ18_08630"
     CDS             <1..181
                     /locus_tag="PLQ18_08630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE01757.1"
                     /translation="VGIDGRGNADAGRETPERAAKFEAVEGSHRRVIPGSWAYWAYWA
                     GPDAGAARPERGRAS"
     gene            complement(270..1157)
                     /locus_tag="PLQ18_08635"
     CDS             complement(270..1157)
                     /locus_tag="PLQ18_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017877258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HNE01758.1"
                     /translation="MDLQALRIFKAVADEGSVTRAAGRLHYVQSNVTARLRGLEEALG
                     TELFHRTGRRLAITPAGRTLLAYAEQILGLAEAARQAVRADDVPQGTLVIGSMETTAA
                     ARLPAALSAFHRAHPQVELQLEPGTTEALLADVLAYRLDCALVAGPQHHPELHAEPVF
                     VEELVLITEAAHPPVGGPRDLHRPQMLSFRSGCAYRGLLERWLIDGGVRPARIASFGT
                     FEAIIGCVAAGMGVSLMPASVVEPHRRLGTIRTHRIEAAYAQVATLLVWRRDQARHAA
                     REAFQATLRACAGSTEQAA"
     gene            1266..2474
                     /locus_tag="PLQ18_08640"
     CDS             1266..2474
                     /locus_tag="PLQ18_08640"
                     /inference="COORDINATES: protein motif:HMM:NF018485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfB/YjiJ family MFS transporter"
                     /protein_id="HNE01759.1"
                     /translation="MSTAELQNPSPSAADERRLLLAGIVALALAMGVGRFLYTPVLPL
                     MLREHALDAAGGGWLASVNFVGYLAGALYAALGVPAARRILVVALALVATALTTLGMG
                     LTDSYALWALLRFIAGVATALAMVLGGALVLERLPAARRASGSGYLYGGIGAGMAMTT
                     LAVAALDRLGVGTAGVWVLSGIATALLLPLCRPLLRPAPAAAAMARTACEAKPVRLGW
                     LVAAYGLAGFGYSVSATFLPVIVRTLGLPALVAESCWLVAGLAAIPASLLWGVIARRR
                     GTEFALAAALATEAAGVALPLLLPNAFGALAGGALFGASFLAVVGLALAVAREAEPTR
                     ASRAMGLMTASFGLGQILAPALSSALIGSGAGFGLPLALAAGALVVALLMLTRLHPQP
                     GVATRLETAP"
     gene            2471..>2928
                     /locus_tag="PLQ18_08645"
     CDS             2471..>2928
                     /locus_tag="PLQ18_08645"
                     /inference="COORDINATES: protein motif:HMM:NF015438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HNE01760.1"
                     /translation="MSTPAADTGIRIVGLFRGRRGVLPEEGQVSAIFREPLNAPCRLG
                     PEGIDGDRPVDRRWHGGPERALLHYPGEHYEYWRRHYPQHAAGFVPGGFGENLTTSGL
                     TEAQVRIGQRFRIGTALVEVSQPRRPCWKLDHRHDLPGLSRAVEATRRSG"
BASE COUNT          349 a         1096 c         1069 g          414 t
ORIGIN      
        1 cgtcggcatc gacggtcgcg gcaatgcaga cgccggtcgc gaaacgcccg agcgtgcggc
       61 gaaattcgag gctgtcgaag gcagtcatcg gcgggtgatc cccggaagct gggcgtactg
      121 ggcgtactgg gcaggcccgg acgcgggggc cgcccggccg gaacgaggcc gcgcatcgta
      181 aggcgcagcc ggcggcaccg ccaccgggca atgccggccg gccgctgccc gcttgaggtg
      241 cagcgggtgc agtctgccgc cgccgccgtt catgccgcct gctccgtgct gcccgcgcag
      301 gcgcgcagcg tcgcctggaa ggcctcgcgc gccgcatgcc gggcctgatc gcgccgccag
      361 accaacagcg tcgcgacctg tgcatacgct gcctcgatgc ggtgcgtgcg gatcgtgccg
      421 agccgccggt gcggctcgac gacgctcgcc ggcatcagcg acacgcccat gccggcggcc
      481 acacagccga tgatcgcctc gaaggtgccg aagctcgcga tgcgcgccgg gcgcacgccg
      541 ccatcgatca gccagcgctc cagcaggccg cggtaggcgc agccgctcct gaacgacagc
      601 atctgcggcc ggtgcaggtc gcgcggcccg ccgaccggcg ggtgcgcggc ctcggtgatc
      661 agcaccagtt cctcgacgaa caccggctcg gcgtgcagtt ccggatgatg ctgcggaccg
      721 gcgaccagcg cgcagtcgag gcggtaggcg agcacgtcgg cgagcagcgc ctcggtcgtg
      781 ccgggttcga gctgcagctc cacctgcgga tgcgcgcgat gaaaggccga cagcgccgcc
      841 ggcagccgcg ccgcggccgt ggtctccatc gaaccgatca ccagcgtgcc ctgcggcacg
      901 tcgtcggcgc gcacggcctg ccgcgcagcc tcggcaagcc cgaggatctg ctcggcgtag
      961 gcgagcagcg tgcgcccggc cggcgtgatc gccagccgcc ggccggtgcg gtggaagagt
     1021 tcggtgccga gggcctcctc cagcccgcgc agccgtgcgg tgacgttcga ctgcacgtaa
     1081 tgcagccgcc cggcggcgcg cgtgacgctg ccctcgtctg cgaccgcctt gaagatgcga
     1141 agcgcctgca ggtccatgat ccatcgctcc cggtgatagg acctgaacag aataattcat
     1201 tttattcgat agaatatccc gactaggctg cgcggacatt ccccaccgtc cgcgagggcg
     1261 ccgcgatgag caccgccgag ctgcagaatc cctccccgag cgcggccgac gaacgccggc
     1321 tgctgctcgc cggcatcgtc gcgctggcgc tggcgatggg tgtcggacgc ttcctgtata
     1381 cgccggtgct gccgctgatg ctgcgcgagc acgcgctcga tgccgccggc ggcggctggc
     1441 tcgcaagcgt caacttcgtc ggttacctcg ccggcgcgct gtacgcggcc ctcggcgtgc
     1501 cggcggcccg gcgcatcctc gtggtcgctc tggcgctggt cgcgaccgcg ctgaccacgc
     1561 tcggcatggg cctgaccgac agctacgcgc tgtgggcgct gctgcgcttc atcgccggcg
     1621 tcgcgacggc gctggcgatg gtgctcggcg gcgcgctggt gctcgaacgc ctgccggcgg
     1681 cgcggcgcgc gtccggctcc ggctatctgt acggcggcat cggtgccggc atggcgatga
     1741 cgacgctcgc ggtcgccgcg ctcgaccggc tcggcgtcgg cacggccggc gtctgggtgc
     1801 tgagcggaat cgcgaccgcg ctgctgctgc cgctctgccg gccgctgctg cgcccggccc
     1861 cggccgccgc ggcgatggcc cgcaccgcct gcgaggcaaa gccggtgcgc ctcggctggc
     1921 tggtggccgc ctacggactc gccggcttcg gctactcggt cagcgccacc ttcctgccgg
     1981 tgatcgtgcg cacgctcggg ctgccggcac tcgttgccga atcctgctgg ctggtcgccg
     2041 ggctggcggc gattccggcg agcctgctgt ggggcgtgat cgcgcgccgg cgcggcaccg
     2101 aattcgcgct ggctgcggcc ctcgccaccg aggccgccgg cgtcgcgctg ccgctgctgc
     2161 tgccgaacgc cttcggtgcg ctcgccggcg gtgcgctgtt cggcgcgagc ttcctcgccg
     2221 tggtcgggct ggcgctcgcc gtcgcccgcg aggccgaacc gacccgcgcc agccgcgcca
     2281 tgggcctgat gaccgcgagc ttcgggcttg gccagatcct cgccccggcg ctgtcctcgg
     2341 cgctgatcgg cagcggtgcc ggcttcggcc tgccgcttgc gctcgccgcc ggcgcgctgg
     2401 tcgtcgcgtt gctgatgctg acgcgcctgc acccgcagcc gggcgtcgcc acgcggctgg
     2461 agacggcgcc gtgagcacgc cggccgcgga caccggcatc cgcatcgtcg gcctgttccg
     2521 cggccggcgc ggcgtgctgc ccgaggaagg ccaggtcagc gcgatcttcc gcgagccgct
     2581 gaatgctcct tgccggctcg gccccgaagg catcgacggc gaccggccgg tcgaccgccg
     2641 ctggcacggc ggtccggagc gcgcgctgct gcactacccc ggcgagcact acgaatactg
     2701 gcgccgccac tacccgcagc acgccgccgg cttcgtgccc ggcggcttcg gcgagaacct
     2761 gacgacgagc ggcctgaccg aggcgcaggt acgcatcggc cagcgctttc gcatcggcac
     2821 ggcgctcgtg gaagtcagcc agccgcgccg gccgtgctgg aagctcgacc atcgccacga
     2881 cctgccggga ctttcccgcg cggtcgaggc gacgcggcgc agcggctg
//
LOCUS       DAOQJH010000358         2927 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_23093_length_2927_cov_3.900418, whole genome shotgun sequence.
ACCESSION   DAOQJH010000358 DAOQJH010000000
VERSION     DAOQJH010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2927)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2927)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2927
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23093_length_2927_cov_3.900418"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            192..1094
                     /gene="tagQ"
                     /locus_tag="PLQ18_08650"
     CDS             192..1094
                     /gene="tagQ"
                     /locus_tag="PLQ18_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010221290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated lipoprotein
                     TagQ"
                     /protein_id="HNE01761.1"
                     /translation="MHALPPQTGARRLAAALSLTLALGGCAGVQLGGPADTVAQTTSV
                     NYYPACYEPVRYLRESDDKMKSAVATGAVGGALAGGLLGALTGGDNAGRNALIGAAAR
                     ALIGGASSYYLEKQKQNAEDSARFAAYGTDIDRATGEYNRTTSAARNAQGCYQKEFQT
                     LVQLRKTRRISDTEGRARFNEIVSGISEANALMAAVDRRIGESLNTYTQAYEEDLKKT
                     GVERQAVTRAARSSKPVAVAGSAKPASTEAAVNTERKVQQASTARDESKAVSSGLTAQ
                     LKSFCSNPDVGDWGSGASACGKGV"
     gene            1168..2049
                     /locus_tag="PLQ18_08655"
     CDS             1168..2049
                     /locus_tag="PLQ18_08655"
                     /inference="COORDINATES: protein motif:HMM:NF024879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper domain-containing protein"
                     /protein_id="HNE01762.1"
                     /translation="MSLSEPKPGQRPLAAALSLLLALSGCAQMNSTMGVVQQTTVNYY
                     PKCYEPVGYLRESDQRMQQAVGTGVALGALGGAAIGAIATGDSRGAIAGAVLGGLAGG
                     ATAYYQQKQQQLAEDSARFAAYGTDIDRTTGEFNRTTSAARNAQRCYQKEFKALVQLR
                     KRGKVSDAEGRARFAEIVSGMSEANALMAAVDGRIGESLDTYTKAYESDLQKRGATRS
                     TVAQSANRGGGRGSSKSSGVPTEAVNVERKVQKATAAREEVKTVTASIAADTRSFCSS
                     PDIGDWGKGNATCAKSA"
     gene            complement(2290..>2927)
                     /locus_tag="PLQ18_08660"
     CDS             complement(2290..>2927)
                     /locus_tag="PLQ18_08660"
                     /inference="COORDINATES: protein motif:HMM:NF025062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNE01763.1"
                     /translation="SFIRDMLDDSDDLAIVEGVIGLARAFDRRVVAEGVETAEHGAVL
                     LAFGCTHAQGYGIARPMPADALPDWLARWQPPALWQHHAARWTRAELPLLGARIELQR
                     WIERLGTCLDLPPGEIGNTPALGTHDCRFGRWYHGPGRQRYGTHPVWAKIDAGHEEFH
                     ALGAELLAMHLAGEAESARARLGELRTQCDALLGLLDALLGSPEREHTDSP"
BASE COUNT          498 a         1031 c         1004 g          394 t
ORIGIN      
        1 gccgacaatc gcctgcgcga gttctgcacc aatccggatg tcggcgactg gctgccgccg
       61 ggcttcgact gcactaggct cagcgtctga atccggctgc cttcgcgatg ccggccgtgc
      121 cgtgctcccg gccagaacca gaacaacaga acaacagaac aacagaacaa cctttacccg
      181 gagtcttccc gatgcatgcc cttcctccgc agaccggtgc ccgcaggctc gccgcggcgc
      241 tgtccctgac cctcgcgctc ggcggttgcg ccggcgtcca gctcggcggc ccggcggata
      301 ccgtcgcgca gacgaccagc gtcaactact acccggcctg ctacgagccg gtgcgctacc
      361 tgcgcgaatc cgacgacaag atgaaatccg ccgtcgccac cggcgcggtc ggcggtgcct
      421 tggccggtgg cctgctcggc gcgctgaccg gcggtgacaa cgccggacgc aacgccctga
      481 tcggcgcggc cgcccgcgcg ctgatcggtg gcgccagcag ctattacctc gaaaagcaga
      541 agcagaacgc cgaggacagc gcgcgcttcg ccgcctacgg caccgacatc gaccgcgcca
      601 ccggcgagta caaccgcacg acctcggccg cgcgcaacgc ccagggctgc taccagaagg
      661 aattccagac gctggtgcag ctgcgcaaga cccgcaggat cagcgacacc gagggtcggg
      721 cgcgcttcaa cgagatcgtc tcgggcatca gcgaggccaa cgcgctgatg gctgccgtcg
      781 accggcgcat cggcgagagc ctcaacacct atacgcaggc ctacgaggaa gacctgaaga
      841 agaccggcgt cgagcgtcag gcggtcaccc gcgccgcccg ttcctcgaag ccggtcgccg
      901 tcgccggctc cgccaagccg gccagcaccg aagccgccgt caacaccgag cgcaaggtgc
      961 agcaggcctc gaccgcgcgc gacgaatcga aggccgtgag cagcggcctg accgcgcagc
     1021 tgaagagctt ctgttccaac cccgatgtcg gcgactgggg cagtggcgcc tcggcctgcg
     1081 gcaagggcgt ctgaatccgc ctggaaccct ctgctgcccg gcacggaagc agcctgagaa
     1141 acccacctgc acaggagtcc gtaggccatg tccctctctg aaccgaaacc cggccagcgt
     1201 ccgctggccg ccgccctgtc gctgctgctg gcgctgagcg gctgcgccca gatgaacagc
     1261 acgatgggcg tcgtgcagca gaccaccgtc aactattacc cgaagtgcta cgaaccggtc
     1321 ggctacctgc gcgagtccga ccagcgcatg cagcaggcgg tcggcaccgg ggtggcgctc
     1381 ggggccctgg gaggagctgc cattggcgcg atagcgaccg gcgattcgcg gggcgccatc
     1441 gccggcgcgg tgctcggcgg tctcgctggc ggcgccacgg cctactatca gcagaagcag
     1501 cagcagcttg ccgaggacag tgcccgcttt gctgcctacg gcaccgacat cgaccgcacg
     1561 accggcgagt tcaaccgcac gacctcggcc gcgcgcaacg cgcagcgctg ctatcagaag
     1621 gagttcaagg cgctcgtgca gctgcgcaag cgcggcaagg tcagcgacgc cgaaggccgg
     1681 gcccgcttcg ccgagatcgt ctcgggcatg agcgaggcca acgcgctgat ggcggcggtc
     1741 gacggccgta tcggtgaaag cctcgacacc tacaccaagg cctacgagtc cgacctgcag
     1801 aagcgcggcg cgacgcgcag cacggttgcg cagtccgcca accgtggcgg cgggcgtggt
     1861 tcgtcgaaga gcagcggtgt gccgaccgag gcggtcaacg tcgagcgcaa ggtacagaaa
     1921 gccacggccg cccgcgagga ggtcaagacc gtcaccgcca gcatcgctgc cgacacgcgc
     1981 agcttctgtt ccagccccga catcggcgac tggggcaagg gcaatgcgac ctgcgccaag
     2041 agtgcgtgag gccgttcata ccggtcccgg ctgagcacgc cgtcttttca gcccctctcc
     2101 cggtgcgggg gagggattgc ggagggagct caacaacaca ttcacgtgcc acgtgtgagc
     2161 ggcatgagcc gctggtgcag gctgccggcc tcgtttgccg gatacaccga tggtgcggct
     2221 ggcgggcgga tcgcgaacat cctgagcttc cggaagccgc atgaagccgc ttgcggctgc
     2281 gatgcagcgt cacggcgaat cggtgtgctc gcgctcggga ctgccgagca gcgcgtcgag
     2341 caggccgagc agcgcgtcgc actgcgtgcg cagctcgccg aggcgcgcgc gcgccgattc
     2401 ggcctcgccg gccaggtgca tggccagcag ttcggcgccg agggcgtgga attcttcgtg
     2461 gccggcatcg atcttcgccc agaccgggtg cgtgccgtag cgctggcggc cggggccgtg
     2521 gtaccagcgg ccgaagcggc agtcgtgcgt gccgagcgcc ggcgtgttgc cgatttcgcc
     2581 gggcggcagg tccagacagg tgccgaggcg ttcgatccag cgctgcagtt cgatgcgcgc
     2641 cccgagcagc ggcagctccg cacgggtcca gcgtgcggcg tgatgctgcc agagcgccgg
     2701 cggctgccag cgtgccagcc agtccggcag cgcgtcggcc ggcatcggcc gggcgatgcc
     2761 gtagccctgc gcatgcgtgc agccgaaggc cagcagcacc gcgccgtgct cggcggtttc
     2821 gacgccttcg gcgaccacgc gccggtcgaa ggcgcgcgcc agaccgatca cgccctcgac
     2881 gatggcaagg tcgtcgctgt catcgagcat gtcgcggatg aaggact
//
LOCUS       DAOQJH010000359         2923 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_23136_length_2923_cov_4.647490, whole genome shotgun sequence.
ACCESSION   DAOQJH010000359 DAOQJH010000000
VERSION     DAOQJH010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2923)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2923)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2923
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23136_length_2923_cov_4.647490"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1951
                     /locus_tag="PLQ18_08665"
     CDS             <1..1951
                     /locus_tag="PLQ18_08665"
                     /inference="COORDINATES: protein motif:HMM:NF017388.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PilC/PilY family type IV pilus protein"
                     /protein_id="HNE01764.1"
                     /translation="WEDHFFDVKNPGELSPALSRLFASVAAGDPSSAALSANVRTLQT
                     SALLFQASYNPADWSGEVKAYGIDAATGALSGLIWKASDHLPATASRNIVTWRPGSGG
                     IAFTWTNLDSTQKAALGNDSTLLDWLRGDQSREGTASNQLRVRTTRLGDVVNSDPVFA
                     GVSDAGYGRLPGSEGASYAAWVTAKESLAQMVYVGANDGMLHGFDATTGSERLAYVPN
                     AVFGRLAGLTARSYSHVYSVDGAPAVGDVHDGSRWRTLLAGTLGAGGKAVFGLDVSDP
                     ATFGPGNVLWEFTDADLGEGVRQALIVRTHDSAHPWVVLFGNGYNGTSGNAVLYALDA
                     LSGVQLAKIVLPVSGASGLATPVAWDADGDLVTDAVYAGDLLGNLWKFDLSGSAGGWR
                     VALGGQPLFTARGPTGLAQPITVQPTVGKGKDGVVRVFFGTGKYLEASDGQIETSPAV
                     QSFYGLVDAGSSITYTSRSSVLTAQTLIAEFPAGTNGPYPLRVSSDNTPADGAKGWYL
                     DLVPPNGVAAGERVVERAVIRYDRIVFPTMLPLASAGCSGGGDGWLMEVLSTTGGRPK
                     TASFDLNNDGRFDGSDGVTINGELVVVTGINPQVGVISGAPAVVVGAKSETKWVTGSA
                     GGTPFSVGERIDARSTGRQSWIQLQ"
     gene            2077..2895
                     /gene="aroE"
                     /locus_tag="PLQ18_08670"
     CDS             2077..2895
                     /gene="aroE"
                     /locus_tag="PLQ18_08670"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HNE01765.1"
                     /translation="MTDRYAVIGNPIAHSKSPRIHTLFAARTGQDLVYEAILGPQGGF
                     ADCATAFRAAGGRGLNVTVPFKQDAWVWADRLSTRAERAGAVNTLKLDEDGVLGDNTD
                     GAGLVRDLETNLGCTLAGRRVLLLGAGGAARGVLAPLLAAGPAAVTIANRTPDKAAEL
                     ALRFGDLGLCYGRSYDELAGERFDLLINATAAGLSGEVPPLPEGLLADAAWCYDMMYG
                     DEPTAFVRWARAHGATHTADGLGMLVEQAAEAFLIWRGVRPDTAPVLAALRGEG"
BASE COUNT          423 a         1050 c         1059 g          391 t
ORIGIN      
        1 gtgggaggac cacttcttcg acgtgaagaa ccccggcgag ctgtcgccgg cgctgagccg
       61 gctgttcgcg tcggtcgccg ccggcgatcc gtcctcggcg gccctgagcg ccaacgtgcg
      121 cacgctgcag acctcggcgc tgctgttcca ggcttcctat aaccccgccg actggagcgg
      181 cgaggtcaag gcctacggca tcgacgccgc caccggcgcg ctgtccgggc tgatctggaa
      241 ggcgagcgat cacctgccgg cgaccgcgag ccgcaacatc gtcacctggc gcccgggcag
      301 cggcggcatc gccttcacgt ggacgaatct cgacagcacg cagaaggcgg cgctgggcaa
      361 cgacagcacg ctgctcgact ggctgcgcgg cgaccagagc cgcgagggca cggccagcaa
      421 ccagctgcgc gtgcgcacga cccgcctcgg cgatgtcgtc aacagcgatc cggtgttcgc
      481 cggcgtcagc gacgccggat acggcaggct gccgggcagc gagggcgcga gctacgcagc
      541 ctgggtcacg gccaaggaaa gcctcgcgca gatggtctac gtcggcgcca acgacggcat
      601 gctgcacggc ttcgatgcga ccaccggcag cgagcgcctc gcctacgtgc cgaacgccgt
      661 gttcggccgc ctcgccggcc tcacggcgcg cagctacagc cacgtctaca gcgtcgacgg
      721 cgcgccggcg gtcggtgatg tccatgacgg cagccgctgg cgcacgctgc tggccggcac
      781 gctcggcgcg ggcggcaagg cggtgttcgg gctcgatgtc agcgatccgg ccacgttcgg
      841 ccccggcaac gtgctgtggg agttcaccga cgccgatctc ggcgagggcg tgcgtcaggc
      901 gctgatcgtg cgcacgcacg acagcgcgca cccgtgggtc gtgctgttcg gcaacggcta
      961 caacggcacg agcggcaacg ccgtgctgta cgcgctcgat gcgctgagcg gcgtgcagct
     1021 cgcgaagatc gtgctgccgg tcagcggcgc cagcggcctc gcgaccccgg tcgcctggga
     1081 tgccgacggc gatctggtca ccgatgccgt ctacgccggc gacctgctcg gcaacctgtg
     1141 gaagttcgac ctcagtggca gcgccggcgg ctggcgggtg gcgctcggcg gccagccgct
     1201 gttcacggcg cgcggcccga ccgggctcgc gcagccgatc accgtgcagc cgaccgtcgg
     1261 caagggcaag gacggcgtcg tgcgcgtgtt cttcggcacc ggcaagtatc tcgaagccag
     1321 cgacggccag atcgagacct cgccggcggt gcagagcttc tacggcctcg tcgatgccgg
     1381 cagcagcatc acctacacca gccgcagcag cgtgctgacc gcgcagaccc tcatcgccga
     1441 gttcccggcc ggcaccaacg gcccgtatcc gctgcgggtc agctccgaca acacgccggc
     1501 cgacggggcg aagggctggt atctggacct ggtgccgccg aacggtgtcg cggccggcga
     1561 gcgtgtcgtc gagcgcgccg tgatccgtta cgaccgcatc gtgttcccga ccatgctgcc
     1621 gcttgcgagc gccggctgct cgggtggcgg cgacggctgg ctgatggagg tgctgtccac
     1681 caccggcggc cggccgaaga ccgcctcgtt cgacctgaac aacgacgggc gcttcgatgg
     1741 cagcgacggc gtgacgatca acggcgagct cgtcgtcgtc accggcatca atccgcaggt
     1801 cggcgtcatc agcggcgcgc cggcggtggt cgtcggcgcc aagtccgaaa ccaaatgggt
     1861 gaccggctcc gccggtggca cgccgttcag cgtcggcgag cgcatcgatg cgcgcagcac
     1921 cgggcggcag tcgtggatcc agctgcagtg agggcgtgac ggcgggacgg cgggaaggac
     1981 agggcggcag cgcgggcgac ggcgtttttt ctcctcccgc cgcccgccgt ccgctcctac
     2041 actgccggcc cccgacctca cccggaagcc gccgtcatga ccgaccgcta cgccgtgatc
     2101 ggcaacccga tcgcccacag caagtcgccg cgcatccaca cgctgttcgc cgcccggacc
     2161 ggacaggatc tcgtctacga ggcgatcctc ggtccgcagg gcggtttcgc cgactgcgcc
     2221 acggcgtttc gcgcagccgg cgggcgcggc ctgaacgtca ccgtgccgtt caagcaggac
     2281 gcctgggtct gggccgatcg cctgagcacg cgcgccgagc gcgccggcgc ggtcaacacg
     2341 ctgaagctcg acgaggacgg cgtgctcggg gacaacaccg acggcgccgg gctggtgcgc
     2401 gacctcgaaa ccaacctcgg ctgcacgctg gccgggcggc gcgtgctgct gctcggggcc
     2461 ggcggcgcgg cgcgcggcgt gctggcaccg ctgctggcgg caggaccggc ggcggtgacg
     2521 attgccaacc gcacgccgga caaggccgcg gaactcgcgc tgcgcttcgg cgacctcggc
     2581 ctctgctacg gccgcagcta cgacgagctg gccggcgaac gcttcgacct gctcatcaac
     2641 gccaccgccg ccgggctttc gggcgaggtg ccgccgctgc ccgaagggct gctcgccgac
     2701 gctgcctggt gctacgacat gatgtacggc gacgaaccga ccgccttcgt gcgctgggcg
     2761 cgcgcgcacg gcgccacgca caccgctgac ggtctcggca tgctggtcga gcaggcggcc
     2821 gaggccttcc tgatctggcg cggcgtgcgt ccggatacgg cgccggtgct ggcggcgctg
     2881 cgcggggaag gctgaagcgg cgcggcaggg caggggccgg aac
//
LOCUS       DAOQJH010000360         2920 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_23179_length_2920_cov_3.220244, whole genome shotgun sequence.
ACCESSION   DAOQJH010000360 DAOQJH010000000
VERSION     DAOQJH010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2920
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23179_length_2920_cov_3.220244"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2226)
                     /locus_tag="PLQ18_08675"
     CDS             complement(<1..2226)
                     /locus_tag="PLQ18_08675"
                     /inference="COORDINATES: protein motif:HMM:NF024486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HNE01766.1"
                     /translation="MHESADALASDLLTLITRHPEGLRASELQARAAIAGRRPRLDAV
                     TAGLLTLQRQNLAGIDAHRRWHLRIPGTAIPASATGEVHAPPAASRGPVLAALPAHGS
                     EGEAWTASAPIHVQAGEQLHPSWAMLRDLLPYYAEALARNERAQLLGASERYGEQFLL
                     VAPGGRWWPTQQEKRVLDIGRAHLPAAFLTALSRRAREPVYIAYPLALVHPKDPQRQP
                     FLLPVTTLAAEWTLDADRLRVILPAQTPAIEWRWASGQRQRGHRIRELLDALDVQADD
                     EAWQAGSFVDWPSFVERLAGAVPADLRTALDPARPVRELDCGGPAGLYGALGLFLSSE
                     LQYARGAVRDLLALGQWSDAEFAATALAPLFTAGSNDGGAMAAAATVPVMEPLALGED
                     QLDAVRDGLNAPLTVVTGPPGTGKSQVAVAVLASAALSGRSVLFASRNHQAIEAVAGR
                     LTELLAHQPLLIRANARDGGEGISFARAIDAILARPGAEARREQLAESITVLSRLDAA
                     RMRALDTATAAALAAEELGRLEATIADREAALGIAQGADLPSVPIPMPAPTPRGWLMR
                     GFAPWLRRLRLRRLRRIALDWQRLGLAAPDESTLELHEQRLAALTELAELRRQRAALE
                     ASVRAGTGAADLEDPVRLGERMQASALQQLQTLAGVLAECAPTDRQALTALRGDVVLA
                     NHDGTAGAARLRERWAAQHTLILGHMPLWAVSNLGAASRIPLVPGLFDYVVIDEAAQC
                     DI"
     gene            complement(2398..>2920)
                     /locus_tag="PLQ18_08680"
     CDS             complement(2398..>2920)
                     /locus_tag="PLQ18_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011386781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNE01767.1"
                     /translation="SWVWDELYRVRNLRPSFRWGLLPGIAYAALDTYLLRGRAPWTLH
                     HHADNASLKTKDRARKIAYPKPDGVVSFDRLSSVFLSNTNHEEDQPAHLRLKDPKIAI
                     DTNLALYDAPEQRYCPAGVYEIVRDADGGNARLQINAQNCVHCKTCDIKDPTQNINWV
                     VPEGGGGPNYPNM"
BASE COUNT          423 a          988 c         1055 g          454 t
ORIGIN      
        1 gatgtcgcac tgggcggctt cgtcgatcac gacgtaatcg aacagccccg gcaccagcgg
       61 gatgcggctg gcagccccga ggttcgatac cgcccataac ggcatatggc cgaggatcag
      121 cgtgtgctgc gcggcccagc gctcgcgcag gcgtgccgcc ccggccgtcc cgtcgtggtt
      181 tgcaagcacg acatcgccgc gcagggccgt cagcgcctga cggtcggtcg gagcgcattc
      241 ggcgagcacg ccggcgaggg tctgcagctg ctgcagcgcc gaggcctgca tccgttcgcc
      301 gagcctgacc gggtcttcga ggtcggcagc accggtgcct gcccgcacgc tggcttccag
      361 ggcggcacgc tgtctgcgca gctcggccag ttcggtgagt gcggcgagtc gctgttcgtg
      421 cagttccagc gtcgattcgt ccggcgcagc caggccgagc cgctgccagt cgagcgcaat
      481 gcgccgcagc cgccgcagcc gcaggcgccg cagccatggg gcgaagccgc gcatcagcca
      541 gccacgcggc gtcggcgccg gcatcgggat gggcaccgat ggcaggtctg ccccctgcgc
      601 aatgccgagt gcagcctccc ggtcggcgat cgtggcttcc agccggccga gttcttcggc
      661 ggccagtgcc gcagccgtcg ccgtgtcgag ggcgcgcatg cgcgcggcat cgagccggga
      721 cagcacggtt atcgattcgg ccagctgctc gcgccgggct tcggcgccgg ggcgggcgag
      781 aatcgcgtcg atcgcacgcg cgaagctgat gccttcgcca ccgtcgcggg cgttggcacg
      841 gatcagcagc ggctgatgtg caagcagttc ggtgagccgt ccggcgacgg cttcgatggc
      901 ctgatggttg cgactggcga acaggacgct gcgcccgctc agcgcggcgc tggccagcac
      961 ggcgactgcg acctgcgact tgccggtgcc gggcgggccg gtcacgacgg tcagcggcgc
     1021 gttgagtccg tcccgcaccg catcaagctg gtcctcgcca agcgcgagcg gctccatgac
     1081 cggtacggtg gcggctgcgg ccatcgcgcc gccgtcgttt gagccagctg tgaacagcgg
     1141 ggccagcgcc gttgcggcga attcggcatc cgaccactgg ccgagtgcaa gcagatcgcg
     1201 taccgcgccg cgggcatact gcagctcgct cgacaggaac aggcccagcg cgccgtagag
     1261 gccggccggc ccgccgcagt cgagttcgcg taccggtcgg gcaggatcga gcgcagtgcg
     1321 cagatccgcc ggcacggctc cggccagccg ctcgacgaag ctcggccagt cgacgaagga
     1381 gccggcctgc caggcttcgt cgtcggcctg cacgtcgagc gcatcgagca gctcccgaat
     1441 ccggtgcccg cgctggcgtt gtccgctggc ccagcgccat tcgatcgccg gggtctgtgc
     1501 cggcaggatc acccgcagac ggtcggcatc cagggtccac tctgcggcca gggtcgtgac
     1561 cggcagcagg aatggctggc gctgcggatc cttcggatgc accagagcca gcggataggc
     1621 gatgtacacc ggctcacgtg cgcgccggga cagggcggtc aggaaggcgg cgggcagatg
     1681 tgcgcggccg atatcgagca cgcgcttttc ctgttgcgtc ggccaccagc ggccgccggg
     1741 cgcaacgagc aggaactgtt ctccgtatcg ctcgctggct cccagcagtt gcgcgcgttc
     1801 gttgcgggcg agtgcctcgg cgtagtacgg cagcagatcg cgcaacatgg cccaggacgg
     1861 atgcagttgt tcgccggcct gcacatggat gggcgctgat gcagtccacg cttcgccttc
     1921 gctgccatgc gccggaaggg cggccaggac cggcccccgg ctggccgctg gcggcgcatg
     1981 gacctcgccg gtcgccgatg ccgggatggc cgtgccggga atgcgcagat gccatcggcg
     2041 atgcgcatcg atcccggcca ggttctggcg ctgcagcgtc agcagtccgg ccgtcacggc
     2101 gtcgagccgg gggcgacggc cggcgatcgc ggcccgggcc tgcaactcgg aggcgcgcag
     2161 gccttccggg tgacgcgtga tcagcgtcag caggtccgaa gccagagcgt cggcggattc
     2221 gtgcacggtc taaacatccc tgcctgggcc tgcacagcgc agggccggat tcgaacggcg
     2281 cagcgcacgc cccggaatca tgaaaatccg tcaatcttat ctgcaagacc gggcctgctt
     2341 caaaaacaga agggaccgtc accggtccct tcgtccatcg ggggcggaca cagccgctca
     2401 catgttcgga tagttcggtc cgccgccgcc ctcgggcacg acccagttga tgttctgggt
     2461 cgggtccttg atgtcgcagg tcttgcagtg gacgcagttc tgcgcgttga tctgcaggcg
     2521 cgcgttgccg ccgtcggcat cgcgaacgat ctcgtagacg ccggccgggc agtagcgctg
     2581 ctccggcgca tcgtagagcg ccaggttggt gtcgatcgcg atcttcggat ccttgagccg
     2641 gagatgcgcg ggctggtctt cctcgtggtt ggtgttcgac aggaacaccg atgacaggcg
     2701 gtcgaagctc accaccccgt cgggcttggg ataggcgatc ttcctggcgc ggtccttggt
     2761 cttgaggctg gcgttgtcgg cgtggtgatg cagcgtccac ggcgcgcggc cgcgcagcag
     2821 ataggtgtcg agcgccgcgt aggcgattcc gggcagcagg ccccagcgga agctcggccg
     2881 caggttgcgc acgcggtaga gctcgtccca cacccagctc
//
LOCUS       DAOQJH010000361         2916 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_23239_length_2916_cov_4.543516, whole genome shotgun sequence.
ACCESSION   DAOQJH010000361 DAOQJH010000000
VERSION     DAOQJH010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2916)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2916)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2916
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23239_length_2916_cov_4.543516"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            198..1220
                     /locus_tag="PLQ18_08685"
     CDS             198..1220
                     /locus_tag="PLQ18_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010098600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HNE01768.1"
                     /translation="MRMSRRTSVLALLAGLIASSAYAAEKLPSFKGDINQTSVSGLSS
                     GAFMAAQLQVAYSATITGAGIVAGGPYYCAAGLIANTSICMGQVPFVAPNPALMVSAA
                     QGFAALGQIDSLDNLKNDKVYIFSGTDDSVVKQVAVDATASFFAYAGVPAENLLYVKT
                     VPAGHAFITPSFGNDCSANAGPYISHCIKDGTGYDQAGAILQHIYGTLKAPVTTLSGK
                     IVEFDQRPFAPAISSMAPTGYLYVPKSCADGAACKVHVAIHGCLQSAAAVGDDVYSRT
                     GYNNWADSNDIVVLYPQVNVSYTIPFNPQGCWDWWGYTGSNYALKSGVQMEAIWTMVQ
                     TLVAKP"
     gene            complement(1512..2402)
                     /locus_tag="PLQ18_08690"
     CDS             complement(1512..2402)
                     /locus_tag="PLQ18_08690"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HNE01769.1"
                     /translation="MNRRPPPPAVTELDPDGLRLSLRERLEFIELRLYWEGRINRGDI
                     AARFGVTLQVATADLNRYQALAPAAIEYDRNAKAYYAAAGFEPRLIRPDAQAWLMQLQ
                     ALGSGLIAPAQGWLGWQPEWATHAQPQRLVAAATLRQVLVAIRERRALHVCYQSMSRP
                     EPHWRWLSPHALAHDGWRWHARAYCHEHGDYRDFVLARFLDLEAPAGDAQGSAPEDID
                     WQTRVRLRIAANPALSEAQRRIIELDYGMTNGSAEIICRRSMLYYTRRQLGLVEGEAA
                     VAADRQLILLEASEEAPGTS"
     gene            2491..>2916
                     /locus_tag="PLQ18_08695"
     CDS             2491..>2916
                     /locus_tag="PLQ18_08695"
                     /inference="COORDINATES: protein motif:HMM:TIGR01596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas3''"
                     /protein_id="HNE01770.1"
                     /translation="MDGKPQDIAAVHYWGKTRPAWHLLPCHCLDVAATGREYLLRHHR
                     LRRCLAAALGLPEPVFLAWFTFFLALHDLGKFAQSFQARRTDVLLRLQPDLDAPTKTS
                     PERHDSLGYGFWNECLRPRLRNGDWLKLGPETPRNRVVPG"
BASE COUNT          488 a          985 c          923 g          520 t
ORIGIN      
        1 tgtcttgaca atgctctgaa ttacaaagga atcaggtagc gttgcacgac cctggtttcg
       61 agggtggcga ctgcgcttgc acgcgggtta taagcaccgg gcggccttta ccggtgacgc
      121 ggcgaaacag ccgcgcgccc gctgccgcga cttcgcacgc atgtttccac ctcccgcacg
      181 tcctgcaagg agctttcatg cgcatgtccc gtcgcacgtc cgttctggcc ctgcttgccg
      241 gcctgatcgc aagttctgct tacgccgccg agaaactgcc gtccttcaag ggcgacatca
      301 atcagacctc ggtgtccggc ctgtcgtccg gtgcgttcat ggctgcgcag ctgcaggtcg
      361 cgtattcggc cacgatcacc ggcgccggca tcgtcgccgg cggcccgtat tactgcgcgg
      421 ccgggctgat tgcgaatacc tcgatctgca tgggacaggt accgttcgtc gcgccgaatc
      481 cggcgctgat ggtatcggcc gcacagggct ttgccgcgct cggccagatc gacagcctcg
      541 acaacctgaa gaacgacaag gtctacatct tcagcggcac cgacgacagc gtcgtcaagc
      601 aggtcgccgt cgatgccacg gcctcgttct tcgcctatgc cggcgtgccg gccgagaacc
      661 tgctgtacgt gaagaccgtg ccggccggcc acgccttcat cacgccgtcc ttcggcaacg
      721 actgcagcgc caacgccggc ccgtacatca gccactgcat caaggacggc accggttacg
      781 accaggccgg cgcgatcctg cagcacatct acggcacgct gaaggcgccg gtgacgacgc
      841 tgagcggcaa gatcgtcgaa ttcgaccagc ggccgtttgc gccggcgatc agcagcatgg
      901 cgccgaccgg ctacctgtac gtgccgaaat cctgcgccga cggcgcggcc tgcaaggtgc
      961 atgtcgccat tcacggctgc ctgcagtcgg ctgccgcggt cggcgacgat gtctacagca
     1021 ggaccggtta caacaactgg gcggacagca atgacatcgt cgtgctgtat ccgcaggtca
     1081 atgtctccta cacgatcccg ttcaatccgc agggctgctg ggactggtgg ggttacaccg
     1141 gctcgaatta cgcactgaaa tccggcgtgc agatggaggc gatctggacg atggtgcaga
     1201 cgctggtcgc gaaaccctga ttcttttgcc ggccacatcc tgccggcccg taccggcacg
     1261 gaaatgacct cggacgccgg attcgcatgc gaatccggcg tttttcatga gtgccactgc
     1321 acaccgatgc cagtttcctg cccgctgcga ttcctgcgga ccgtgatgcc ggtcacaccg
     1381 gaacgccttg tctttcagcc cctcgcccct tgtgggagag gagttgggga gcgggggaga
     1441 aaagccatgc attcacgtgt gatcggtatg atcagtgtta tcagcccttc gcgccctgtg
     1501 gcagatacga ttcaggaagt gcccggcgct tcctcagagg cctccagcag gatcagctgc
     1561 cgatccgcag cgactgcggc ctcgccctcc acgagaccga gctgccggcg cgtgtaatag
     1621 agcatcgagc gccggcagat gatttccgcc gatccattcg tcatgccgta gtccagctcg
     1681 atgatccggc gctgcgcctc gctcagggcc ggattggcag cgatgcgcag ccttacccgg
     1741 gtctgccagt cgatgtcttc cggcgcactg ccctgcgcat cgcccgccgg cgcctcgagg
     1801 tcgagaaagc gcgcgagcac gaaatcccgg taatcgccgt gctcatggca gtacgcccgc
     1861 gcatgccagc gccagccgtc atgcgcgagc gcgtgcgggc tcagccagcg ccagtgcggt
     1921 tcggggcggg acatcgactg gtagcacacg tgcagggccc gccgctcgcg gatcgcgacc
     1981 agcacctgac gcagcgtggc cgccgccacc agacgctgcg gctgcgcatg cgtggcccac
     2041 tccggctgcc agccgagcca gccctgggcc ggcgcgatca gaccgctgcc gagggcctgc
     2101 agctgcatca gccaggcctg cgcatcgggg cggatcagcc gcggttcgaa gccggccgcg
     2161 gcgtagtacg ccttggcgtt gcggtcgtac tcgatcgccg cgggcgccag cgcctgatag
     2221 cggttcagat cggccgtcgc cacctgcagc gtcacgccga agcgcgccgc gatgtcgccg
     2281 cggttgatgc ggccttccca gtacaggcgc agctcgatga attcgagccg ctcgcgcagc
     2341 gacaggcgca gcccgtcggg atcgagttcg gtaacagcgg ggggcggggg gcgtctgttc
     2401 atatgtaaaa tatatcagta aaaaaaacag atggactttt tgacttgatg gattaggctt
     2461 gctgccgttg tcgcttcggg agggatcgac atggatggca agccacagga catcgcagcc
     2521 gtgcactact ggggcaagac gcgccccgcc tggcatctgc tgccctgcca ctgtctggat
     2581 gtggccgcga ccgggcgcga atacctgctg cgtcaccatc gtctgcgccg ctgcctcgcc
     2641 gctgcgctgg gtctgccgga accggtgttc ctcgcgtggt tcaccttctt cctcgcgctg
     2701 catgacctcg gcaagtttgc gcagagcttc caggcccggc gcaccgatgt cctgctgcgg
     2761 ctgcagcctg atctcgacgc cccgaccaag acttcgccgg agcgccatga cagcctcggc
     2821 tacggcttct ggaacgagtg cctgcgcccg cgcctgcgca acggcgactg gctgaagctc
     2881 ggcccggaaa cgccgcgcaa ccgcgtcgtg cccggc
//
LOCUS       DAOQJH010000362         2910 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_23310_length_2910_cov_3.633625, whole genome shotgun sequence.
ACCESSION   DAOQJH010000362 DAOQJH010000000
VERSION     DAOQJH010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2910)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2910)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2910
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23310_length_2910_cov_3.633625"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..430)
                     /locus_tag="PLQ18_08700"
     CDS             complement(<1..430)
                     /locus_tag="PLQ18_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018952806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HNE01771.1"
                     /translation="MPAALRSDWSLADWLAWQETLHPKGIDLGLERVRAVAARLDLLQ
                     PSARTIVVGGTNGKGSTLAFLEAMLLAAGRRVAAYTSPHLLRYEERLRLDGRECPAGD
                     WCRAFAAVDAARGDQSLTYFEFGTLAALWLARAFDPEVLVL"
     gene            complement(435..1319)
                     /gene="accD"
                     /locus_tag="PLQ18_08705"
     CDS             complement(435..1319)
                     /gene="accD"
                     /locus_tag="PLQ18_08705"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018716951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HNE01772.1"
                     /translation="MSWFEKLMPSRIRTEGGNKRQIPEGLWSKCDACSAVLYRAELER
                     NLHVCPKCDHHMRIRARERLASFLDADGRVEIGAEVEPTDFLKFKDSKKYKDRLAAAQ
                     KSTGEKDALVAMSGTVLGVPVVASAFDFEFLGGSMGSVVGERFVRAARVALETRVPYV
                     CFTASGGARMQEALVSLMQMAKTSAILARLAEAGVPFVSVLTDPTTGGVSASLAMLGD
                     LNVAEPKALIGFAGPRVIEQTVREKLPEGFQRSEFLLEHGAVDLIIDRRELRARLGAI
                     LAMLSGRPQPLAQAEAAV"
     gene            complement(1397..2209)
                     /gene="trpA"
                     /locus_tag="PLQ18_08710"
     CDS             complement(1397..2209)
                     /gene="trpA"
                     /locus_tag="PLQ18_08710"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011628981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HNE01773.1"
                     /translation="MNRIQTRFDALRTAGRRALIPYITAGDPDLGTTETLLHTLVASG
                     ADIIELGVPFSDPMADGPVIQRACERALAAGTRLRDVLALVRRFRERDADTPVVLMGY
                     LNPVEAMGYAAFAEAGAQAGVDGVLTVDLPPEEAHELTQALLASDLDPIFLLAPTSTE
                     GRIRRIGALARGYLYYVSLKGVTGSARLNTGEVAEKVARIKALVDVPVGVGFGIRDAD
                     SAAAVARVADAVVVGSALVSRIEALAGDPQQLNAEIGVLLGAMRTAMDAVTA"
     gene            complement(2223..>2910)
                     /locus_tag="PLQ18_08715"
     CDS             complement(2223..>2910)
                     /locus_tag="PLQ18_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HNE01774.1"
                     /translation="KDALNEALRDWVTNVDDTFYVIGTVAGPHPYPMMVRDFQCIIGR
                     EAREQVLAGFGRLPDALVACVGGGSNAIGLFHPFLNDASVAMYGVEAAGDGLDTGRHS
                     APLNAGRPGVLHGNRTYLMEDENGQIIETHSVSAGLDYPGVGPEHAWLKDIGRVRYGA
                     ITDDEALAAFHELCRTEGIIPALESSHALAYAKKLAPTLAADQLLVVNLSGRGDKDIF
                     TVAARDGITI"
BASE COUNT          457 a         1003 c         1035 g          415 t
ORIGIN      
        1 cgagcacgag cacttcggga tcgaaagccc gcgccagcca gagcgccgcc agcgtgccga
       61 attcgaaata ggtcagcgac tggtcaccgc gggccgcgtc gaccgccgcg aacgcccggc
      121 accagtcgcc ggccggacat tcgcggccat cgaggcgcag gcgctcctcg tagcgcagca
      181 gatgcggcga ggtgtaagcc gcgacgcgcc ggccggcggc gagcagcatc gcttcgagaa
      241 aggcaagcgt cgaacccttg ccgttggtgc cgccgaccac gatcgtgcgc gccgagggct
      301 gcagcaggtc gagcctggcc gccaccgcac gcacgcgctc cagcccgaga tcgatgccct
      361 tcggatgcag ggtttcctgc caggccagcc agtcggcgag cgaccagtcg ctgcgaagcg
      421 ccgcgggcat gtgctcagac cgcggcttcg gcctgggcga gcggctgcgg acgtcccgag
      481 agcatcgcga ggatcgcgcc gaggcgcgca cgcagttcgc gccggtcgat gatcaggtcg
      541 accgcgccgt gttcgagcag gaattcgctg cgctggaagc cttcgggcag cttctcgcgt
      601 acggtctgct cgatgacgcg cgggccggcg aagccgatca gtgccttcgg ctcggcgaca
      661 ttcagatccc cgagcatcgc cagactcgcc gaaacgccgc cggtggtcgg atcggtcagc
      721 accgacacga acggcacgcc ggcctcggcg agccgcgcca ggatggcgct ggtcttggcc
      781 atctgcatca gcgacaccag cgcctcctgc atgcgcgcac cgcccgaggc cgtgaagcac
      841 acatagggga cccgcgtttc cagcgccacg cgcgcggcgc gcacgaaacg ctcgccgacc
      901 accgagccca tcgagccgcc gaggaactcg aagtcgaagg ccgaggccac gaccggcacg
      961 ccgagcaccg tgccgctcat ggcgaccagc gcatccttct cgccggtgga tttctgcgcg
     1021 gcggccaggc ggtccttgta cttcttgctg tccttgaact tcaggaagtc ggtcggctcg
     1081 acctcggcgc cgatctcgac gcggccgtcc gcatcgagga agctcgccag ccgctcgcgc
     1141 gcgcggatgc gcatgtgatg gtcgcacttc gggcagacgt gcaggttgcg ctccagctcg
     1201 gcgcggtaca gcacggcgct gcaggcatcg cacttcgacc agagcccttc gggaatctgg
     1261 cgcttgttgc cgccctcggt gcggatgcgc gagggcatga gtttttcgaa ccagctcatg
     1321 tcgaccatgg cttgcacaac actcccgcca gcaaagcggc cggcgtcagg aatacggaaa
     1381 aggatcgcgg gccggctcag gccgtgacgg catccatcgc ggtgcgcatc gcaccgagca
     1441 gcacgccgat ctcggcgttc agctgctgcg gatcgccggc cagcgcctcg atgcgcgaga
     1501 ccagcgcgct gccgacgacg acggcatcgg cgacgcgcgc gaccgcggcg gcgctgtcgg
     1561 catcgcggat gccgaagccg acgccgaccg gcacatcgac cagcgccttg atgcgcgcga
     1621 ccttttcggc aacctcgccg gtattgagcc gggccgagcc ggtcacgccc ttcagcgaca
     1681 cgtagtacag gtagccgcgc gccagcgcac cgatgcggcg gatgcgcccc tcggtgctgg
     1741 tcggcgcgag caggaagatc ggatcgaggt cgctggcgag cagcgcctgc gtcagctcgt
     1801 gggcttcctc gggcggcagg tcgacggtca gcacgccgtc gacgccggcc tgcgcgccgg
     1861 cctcggcgaa ggccgcgtag cccatcgcct cgaccgggtt caggtagccc atcagcacga
     1921 ccggcgtgtc ggcatcccgc tcgcggaagc gccggaccag cgccagcacg tcgcgaaggc
     1981 gcgtgccggc ggcgagtgcg cgctcgcagg cacgctggat caccgggccg tcggccatcg
     2041 ggtccgagaa cggcacgccg agttcgatga tgtcggcacc gctggcgacc agcgtgtgca
     2101 gcagggtttc ggtggtgccg agatccggat cgccggcggt gatgtacggg atcagcgcgc
     2161 ggcgaccggc cgtgcgcagg gcgtcgaatc gggtctggat gcggttcatg gcggcccggc
     2221 tctcagatcg tgatgccgtc gcgcgcggcg acggtgaaga tgtccttgtc gccgcgaccg
     2281 gacaggttga cgacgagcag ctggtcggcg gccagcgtcg gggccagctt cttcgcgtag
     2341 gcaagtgcgt gcgagctttc gagcgccggg atgatgccct cggtgcggca gagctcgtgg
     2401 aaggcggcga gcgcctcgtc gtcggtgatc gccccgtagc gcacgcggcc gatgtccttc
     2461 agccaggcgt gctcgggacc gacgccggga tagtcgagac cggccgagac cgagtgcgtc
     2521 tcgatgatct ggccgttctc gtcttccatc aggtaggtgc ggttgccgtg cagcacgccg
     2581 gggcgaccgg cgttcagcgg ggccgaatgg cgaccggtgt cgaggccatc gccggccgcc
     2641 tcgacgccgt acatcgcgac cgaggcgtcg ttgaggaagg gatggaacag gccgatggcg
     2701 ttcgagccgc cgccgacgca ggccaccagc gcatcgggca gccggccgaa accggcgagc
     2761 acctgttcgc gcgcctcgcg gccgatgatg cactggaagt cgcgcaccat catcggatac
     2821 gggtgcggac cggcgacggt gccgatcacg tagaaggtgt catcgacgtt ggtgacccag
     2881 tcgcgcagcg cctcgttcag cgcatccttc
//
LOCUS       DAOQJH010000363         2896 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_23496_length_2896_cov_5.157339, whole genome shotgun sequence.
ACCESSION   DAOQJH010000363 DAOQJH010000000
VERSION     DAOQJH010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2896)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2896)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2896
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23496_length_2896_cov_5.157339"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1277)
                     /locus_tag="PLQ18_08720"
     CDS             complement(<1..1277)
                     /locus_tag="PLQ18_08720"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNE01775.1"
                     /translation="MNDDAALLQALFRAIPDMVWLKDIDGRYLACNPAFERYYGQMAE
                     AILGHTDFDFVDADTAAFYRRKDAEAAAAGVPRRNEEWIVYPDGARRLIETTKTPLHT
                     GNGQLLGVLGIARDITERKQAEEAALDQQARLRALLDSFPFPVWMKDADCRFVAVNRA
                     VALLTGHADPAALIGHEDLDFFPPDLCARYRADDLAVLRDGLPRQVEEPIEARGERRW
                     FETLKFPAFGTDDRVIGTVGYARDVTVRRDAEERLRAYERDYRHFFEDGRVAMLLVDP
                     ADGSIVDANHAAAIFYGWSRPQLLGLNLSGLSMLPAGALASRIEEAVAAAQTHFLCRH
                     RRADGSEPDVEVHAAPVRARGRSLLYLIVHDVTERVALQSALADSEARYRLLFENMHE
                     LLFDIAPDATVRLLNPAWTTITGFFCSTMATLGA"
     gene            complement(1415..1813)
                     /gene="pcaC"
                     /locus_tag="PLQ18_08725"
     CDS             complement(1415..1813)
                     /gene="pcaC"
                     /locus_tag="PLQ18_08725"
                     /EC_number="4.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006086103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047575 - 4-carboxymuconolactone
                     decarboxylase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-carboxymuconolactone decarboxylase"
                     /protein_id="HNE01776.1"
                     /translation="MSPEQFEKGLEVRRAVMGDAFVERALANLDDFSRPLQELVTGYA
                     WGEIWTRPGLDRRTRSLINLAMLTALNRGTEFKGHVRGAINNGCTREEIVEVLLQAAV
                     YCGMPAGVEAFRNAREVLDQIDAERAGGSQ"
     gene            1978..2178
                     /locus_tag="PLQ18_08730"
     CDS             1978..2178
                     /locus_tag="PLQ18_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010222926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2788 domain-containing protein"
                     /protein_id="HNE01777.1"
                     /translation="MTEAEFADWSLRLLLPALIGWMGFIVWGLARESKAGKFGTFVMF
                     GVLGLGVLGFIAKNVITFMLGL"
     gene            complement(2203..>2896)
                     /locus_tag="PLQ18_08735"
     CDS             complement(2203..>2896)
                     /locus_tag="PLQ18_08735"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019725993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNE01778.1"
                     /translation="IVNGRHADAVEQACAAIRAAVAGAEVSGISADVGSAEGCAQLLA
                     ALPAVDVLVNNVGIYGPQDFFEVSDETWQRFFEVNVLSAVRLARAYVPGMATRGWGRV
                     VFLSSESGLNIPADMIHYGFTKTALLSVSRGLAKRMAGSGVTVNAVLPGPTLTEGVQA
                     MLQAEVEKTGQPIEAVGAAFVAAHRPSSILRRLASAEEVANLIVYACSPQASATTGAA
                     LRVDGGCVDTIA"
BASE COUNT          427 a         1003 c         1013 g          453 t
ORIGIN      
        1 gcgcccaacg tggccatcgt cgagcagaag aagccggtga tcgtggtcca ggccggattg
       61 agcaggcgca cggtcgcatc cggtgcgatg tcgaacagca gttcgtgcat gttctcgaac
      121 agcagccggt agcgcgcctc gctgtcggcg agcgccgact gcagggcgac gcgctcggtg
      181 acatcgtgga cgatcaggta cagcagcgag cgtccgcgtg cgcgcaccgg tgccgcatgc
      241 acctcgacat cgggctcgct gccatcggcc cgccggtggc ggcagaggaa gtgcgtctgc
      301 gcggcagcca ccgcctcctc gatccggcta gcgagggctc cggccggcag catgctcagg
      361 ccgctcaggt tcagcccgag cagctgcggc cgtgaccagc cgtagaagat cgcggcggcg
      421 tggttggcat cgacgatgct gccgtcggcc ggatcgacca gcagcatcgc gacgcggccg
      481 tcctcgaaga aatgccggta gtcgcgctcg taggcgcgca ggcgttcctc ggcatcgcgg
      541 cgcacggtca cgtcgcgcgc gtagccgacg gtgccgatca cgcggtcatc ggtgccgaac
      601 gccgggaact tcagggtctc gaaccagcgc cgctcgccgc gggcctcgat cggttcctcg
      661 acctggcgcg gcaggccgtc gcggagcacg gccagatcgt cggcgcggta gcgggcgcac
      721 agatcgggcg ggaagaagtc caggtcctcg tgcccgatca gcgccgccgg gtccgcgtga
      781 ccggtcagca gcgccacggc gcggttgacg gcgacgaagc ggcagtcggc atccttcatc
      841 cacaccggga acgggaagct gtcgagcagc gcgcgcagcc gcgcctgctg gtcgagcgcg
      901 gcctcctcgg cctgcttgcg ctcggtgatg tcgcgggcga tgccgagcac gccgagcagc
      961 tggccgttgc cggtgtgcag cggcgtcttg gtggtctcga tcaggcggcg cgcgccgtcg
     1021 ggatagacga tccattcctc gttgcgtcgc ggcacgccgg cggcggcggc ttcggcgtcc
     1081 ttgcgccggt agaacgccgc cgtgtcggca tcgacgaaat cgaagtcggt gtgcccgagg
     1141 atggcctcgg ccatctggcc gtaatagcgc tcgaaggccg gattgcaggc cagatagcgg
     1201 ccgtcgatgt ccttcagcca gaccatgtcc gggattgccc ggaacagcgc ctgcagcagc
     1261 gcggcgtcat cgttcatgtc cgcccccctt cggacccggt ggcgcacggc ggccgcaatg
     1321 gcccggaccg gcggaccgcc agcgtaggcg gcagcggcgg ctggcgatag ctccggtatc
     1381 ggccgccgcc gtcaattcct gaaacggcgc agggtcattg tgatccgccg gcgcgctcgg
     1441 cgtcgatctg gtcgagcact tcccgggcat tgcggaaggc ttcgacgccg gccggcatgc
     1501 cgcagtagac ggctgcctgc agcagcacct cgacgatctc ctcgcgcgtg cagccgttgt
     1561 tgatcgcgcc gcgcacgtgg cccttgaact cggtgccgcg gttgagcgcc gtcagcatcg
     1621 ccaggttgat caggctgcgc gtgcgccggt cgaggccggg ccgcgtccag atctcgcccc
     1681 aggcgtagcc ggtcacgagt tcctgcagcg ggcggctgaa gtcatcgagg ttcgcgagcg
     1741 cacgctcgac gaaggcatcg cccatcacgg cgcggcggac ttcgagacct ttttcgaact
     1801 gttccgggct catggcggta ctccgggtca aggggcaggg gcagcaggga cagtctggat
     1861 gcggcggtgc agcatggtct tgacctgcat caaagcacag cccggcgccg atcggcagac
     1921 tcgcgcgtcc atcacgcttt ccgaatccgc ccgcctgcca ccgtttccgg agtccacatg
     1981 accgaagccg aatttgccga ctggtcgctg cgcctgctgc tgcctgccct gatcggctgg
     2041 atgggattca tcgtctgggg gctggcgcgc gagtccaagg ccggcaagtt cggcaccttc
     2101 gtgatgttcg gcgtgctcgg cctcggcgtg ctcggcttca tcgccaagaa cgtcatcacc
     2161 ttcatgctcg gactctgagc acgcccggcc acccgttgtg cttcaggcga tcgtgtcgac
     2221 gcagccgcca tcgacgcgca gcgccgcgcc ggtggtggcc gaggcctgcg gcgagcaggc
     2281 gtagacgatc aggttcgcaa cctcctcggc gctcgcgagc cggcgcagga tcgaactcgg
     2341 ccggtgtgcg gcgacgaagg ccgcaccgac cgcctcgatc ggctgaccgg tcttctcgac
     2401 ttcggcctgc agcatcgcct gcacgccttc ggtcagcgtc gggccgggca gcaccgcatt
     2461 gacggtgacc ccgctgccgg ccatgcgctt ggccagcccg cgcgacaccg acagcagcgc
     2521 ggtcttcgtg aagccgtaat ggatcatgtc ggccgggatg ttcaggcccg attccgagga
     2581 caggaacacc acccgccccc agccgcgcgt cgccatgccc ggcacgtagg cccgcgccag
     2641 ccgcacggcg gacagcacgt tgacctcgaa gaagcgctgc caggtctcgt cgctcacttc
     2701 gaagaaatcc tgcggaccgt agatgccgac gttgttgacc agcacgtcga ccgccggcag
     2761 cgcggccagc agctgcgcac agccttcggc gctgccgaca tcggcgctga tgccgctgac
     2821 ctcggcgcct gcgactgcgg cgcggatcgc cgcgcaggcc tgctcgacgg cgtcggcgtg
     2881 ccggccgttg acgatg
//
LOCUS       DAOQJH010000364         2896 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_23504_length_2896_cov_2.541359, whole genome shotgun sequence.
ACCESSION   DAOQJH010000364 DAOQJH010000000
VERSION     DAOQJH010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2896)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2896)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2896
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23504_length_2896_cov_2.541359"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..235)
                     /gene="yidD"
                     /locus_tag="PLQ18_08740"
     CDS             complement(<1..235)
                     /gene="yidD"
                     /locus_tag="PLQ18_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020565499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HNE01779.1"
                     /translation="MAAGEEAPGTARPAGVAQRLALLLIGAYRRWLSPYKGFRCAHAA
                     LHGGASCSQAVADIVAGHGLWQGRQHIRARFRAC"
     gene            complement(374..622)
                     /locus_tag="PLQ18_08745"
     CDS             complement(374..622)
                     /locus_tag="PLQ18_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HNE01780.1"
                     /translation="MKRALIPVAALAFAALVSGCSTVDRTVSAAQHGDWKCVGGTVAG
                     LAVGGIAGHGIGAGTGRTLATVGGAAAGGYVGNQLGCN"
     gene            complement(874..1398)
                     /locus_tag="PLQ18_08750"
     CDS             complement(874..1398)
                     /locus_tag="PLQ18_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020203214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HNE01781.1"
                     /translation="MNPQPPEAADADADPTADAALERALILATQAGLPLTPRPFATLA
                     EQLGSDADTVRAGFAAMQARGAIRRIGALPDHYALGYVANGMTVWDIDDAQLRDCGRR
                     LGALGFVSHCYHRPRSLPAWPYNLFAMVHAHDRTEVEARRAEMRALLGSACRADAVLY
                     STRILKKTGLRIGA"
     gene            complement(1395..1679)
                     /locus_tag="PLQ18_08755"
     CDS             complement(1395..1679)
                     /locus_tag="PLQ18_08755"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HNE01782.1"
                     /translation="MQAIPYTDARNQLARLLDRVTREHQPVLITRRKGEPAVLIALAD
                     FEHWRHEHYLLRRPDEARRLLDTLDEIRDGRHGEPQDPPQDGIDRAGTQP"
     gene            complement(1708..2181)
                     /locus_tag="PLQ18_08760"
     CDS             complement(1708..2181)
                     /locus_tag="PLQ18_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AsnC family transcriptional regulator"
                     /protein_id="HNE01783.1"
                     /translation="MSRPAATRPALDAADRALINRLQRGLPLVDAPYAAVGAELGLSE
                     AEVLQRLQSLLDRRVLTRFGPLFHAERLGGALLLAALAVPQDDFERIAALVNAFPEVA
                     HNYEREHALNMWFVLATATPAALEAAILGIEAATGLPVHRFPKLEEFYVGLYLPA"
     gene            complement(2178..2708)
                     /locus_tag="PLQ18_08765"
     CDS             complement(2178..2708)
                     /locus_tag="PLQ18_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005964498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsnC family protein"
                     /protein_id="HNE01784.1"
                     /translation="MNPPQLPEASRAAPAGAWRQALIAAVQDGLPLVPEPFAALAATL
                     GCDEAEVRAGLAALLADGTVRRLGVVVRHRELGFAANAMVVWDVPDTRVDALGAQLAA
                     EPAVTLCYRRPRVPPHWPYNLFCMIHGHDRAAVLAELAAIVGRHGLGELPHRPLFSRQ
                     RFKQRGARYLDRGAPS"
     gene            complement(2705..>2896)
                     /locus_tag="PLQ18_08770"
     CDS             complement(2705..>2896)
                     /locus_tag="PLQ18_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006475730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HNE01785.1"
                     /translation="EVNHNYEREHRFNLWFVVTAGSEGRLQAALGAIERATGLPVLRL
                     PLLQEYHIDLGFRLHGGRP"
BASE COUNT          404 a          940 c         1159 g          393 t
ORIGIN      
        1 ggcaggcgcg aaagcgggcg cggatgtgct gcctgccctg ccagagaccg tgtccggcaa
       61 cgatgtcggc gaccgcctgc gagcacgagg cgccgccgtg cagcgcggcg tgggcgcagc
      121 ggaaaccctt gtacggcgac agccagcgcc ggtaggcgcc gatcagcagg agcgccagcc
      181 gctgcgcgac gcccgcgggc cgggcggtgc cgggcgcttc ctcacctgct gccatcgctg
      241 ctcctgcgcc gtgagcgttt tgtcgatggc gccagtctgg cggccggtgg cgggcgggtg
      301 aggcagcgtc caaaaaaaac cgccgcgtgc attcacgcgg cggcgggggg agacgggggc
      361 ggggcggagt ggatcagttg cagccgagct ggttgccgac gtaaccgccg gcggcggcgc
      421 caccgacggt ggcgagcgtg cggccggtgc cggcaccgat gccgtgaccg gcgatgccgc
      481 cgacggcgag gccggcgacc gtgccgccga cgcacttcca gtcaccatgt tgggcggcgc
      541 tgacggtgcg atcgacggtg gaacagccgc tgacgagggc agcgaaggca agggcggcga
      601 ctgggatcag ggcgcgcttc atgatgggat gctcctcttg gggcggcgcg gggccgttat
      661 gaagatgctg cttctgatgc cggaagacgg ggataagttc gattgcgaat cccctgcgtc
      721 cagcgtagcc acgtccggcg cagcgcgcag gcgatcgggc gaatggattt gcacggctca
      781 ggccgatcgg gcaggacgat ggcaaggcat tgacgagcaa gcccgtccgc cttcgcgggg
      841 gaagggttgg ggagagggga cgaagccacg gtatcaggcg ccgatgcgca ggcccgtctt
      901 cttcaggatg cgcgtgctgt agagcacggc atcggcgcgg caggcgctgc cgagcagcgc
      961 gcgcatttcg gcgcggcggg cctcgacttc ggtgcggtcg tgcgcatgca ccatcgcgaa
     1021 caggttgtac ggccaggccg gcaggctgcg cgggcggtgg tagcagtggc tgacgaagcc
     1081 gagcgcgccg aggcggcggc cgcagtcgcg cagctgcgca tcgtcgatgt cccagaccgt
     1141 catgccgttg gcgacgtagc cgagcgcgta gtgatcgggc agcgcgccga tgcgccggat
     1201 cgcgccgcgt gcctgcatcg ccgcgaagcc ggcacgcacg gtgtcggcgt cgctgccgag
     1261 ctgttccgcc agcgttgcga acggccgcgg cgtcagcggc agcccggcct gggtggcgag
     1321 gatcagcgcg cgttcgaggg cggcgtcggc ggtggggtcg gcgtcggcgt cggcagcctc
     1381 gggcggctgc ggattcacgg ctgcgtaccg gcgcggtcga tgccgtcctg cggcgggtcc
     1441 tgcggctcgc cgtgccggcc gtcgcggatc tcgtcgaggg tgtcgagcag gcggcgggcc
     1501 tcgtcggggc ggcgcagcag gtaatgctcg tggcgccagt gctcgaagtc ggcgagcgcg
     1561 atcagcaccg ccggttcgcc cttgcgccgg gtgatcagta ccggctggtg ctcgcgcgtg
     1621 acgcgatcga gcaggcgggc gagctggttg cgggcatcgg tatacggaat cgcttgcatc
     1681 ggggggctcc cagcgcgggg ctgcggttca cgccggcagg tacaggccga cgtagaactc
     1741 ttcgagtttc ggaaaacggt ggaccggcag gccggtggcg gcctcgatgc cgaggatcgc
     1801 ggcttcgagg gcggccggcg ttgcggtggc gagcacgaac cacatgttca gcgcgtgctc
     1861 gcgctcgtag ttgtgcgcga cctcggggaa ggcgttgacg agggcggcga tgcgctcgaa
     1921 gtcgtcctgc ggcaccgcga gggctgcaag cagcagcgcg ccgccgagcc gctcggcatg
     1981 aaacagcggc ccgaagcgcg tcagcacgcg ccgatcaagc aggctttgca gccgctgcag
     2041 tacctcggcc tcgctcaggc cgagttcggc cccgaccgcc gcatacggcg catcgaccag
     2101 cggcaggccg cgctgcaggc ggttgatcag cgcgcggtcg gcggcgtcga gcgcaggccg
     2161 cgtggcggcg ggccggctca tgacggggct ccgcggtcga ggtagcgcgc gccgcgctgc
     2221 ttgaagcgct ggcggctgaa cagcggccgg tgcggcagtt cgccgagacc gtggcgcccg
     2281 acgatcgccg ccagttccgc gagcacggcg gcgcggtcgt gcccgtggat catgcagaac
     2341 aggttgtacg gccagtgcgg cggcacgcgc gggcggcggt agcagagcgt caccgccggc
     2401 tcggcggcga gttgtgcgcc gagcgcatcg acgcgcgtgt ccggcacgtc ccagaccacc
     2461 atggcgttgg ccgcaaagcc gagttcgcgg tggcgcacga ccacgccgag ccggcgcacg
     2521 gtgccatcgg cgagcagcgc ggcgaggccc gcgcgcacct cggcctcgtc gcagccgagc
     2581 gtggcggcga gggctgcgaa cggttccggc accagcggca ggccgtcctg cacggcggcg
     2641 atcagcgcct gacgccacgc tccggccggg gcggcgcgcg aggcctccgg gagctgcggc
     2701 ggattcatgg ccggcctccg tgcagccgga agccgaggtc gatgtggtac tcctggagca
     2761 gcggaaggcg cagcaccggc aggcccgtcg cccgttcgat ggcgccgagc gcggcctgaa
     2821 gtcgcccctc ggatccggcg gtgacgacga accacaggtt gaagcggtgc tcacgttcgt
     2881 agttgtgatt gacctc
//
LOCUS       DAOQJH010000365         2894 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_23528_length_2894_cov_3.692145, whole genome shotgun sequence.
ACCESSION   DAOQJH010000365 DAOQJH010000000
VERSION     DAOQJH010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2894)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2894)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2894
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23528_length_2894_cov_3.692145"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1000)
                     /locus_tag="PLQ18_08775"
     CDS             complement(<1..1000)
                     /locus_tag="PLQ18_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007148726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HNE01786.1"
                     /translation="MTQTPADDRPAPGPLAGLRVLELGQLIAGPFTGTLLAYFGAEVI
                     KIEPPEGDPIRQWRVVRDGTSLWWHSIGRNKKSVRIDLKTGEGRALVGQLAARCDVLV
                     ENFRPGTLEKWGLGPEVLKAANPGLVIARISGYGQDGPYAGKAGYASVCEGFGGLRYV
                     NGMPGEPPVRPNLSIGDTLAALHAVIGILMALHQRGRSGSGQVVDVALFEAVYNLLES
                     VVPEFDGAGVVREPSGTTVTGIVPTNTYRCADGRWAIIGGNGDSIYKRLMRAAGRADL
                     ADDPRLANNPGRVQHEAEIDAAIAAWTATLPLAEVVSSLETAGVPAGPIYSVRDMFA"
     gene            1337..>2894
                     /gene="recJ"
                     /locus_tag="PLQ18_08780"
     CDS             1337..>2894
                     /gene="recJ"
                     /locus_tag="PLQ18_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003246113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HNE01787.1"
                     /translation="MPTAIVRRPLPSPGERPELPADLPPLLARVYALRGVASARELDQ
                     ALTALPAPQRLKGIDRAVDLLSAALSTDARILIVADYDADGATACALAVRGLRALGAR
                     HVDYLVPNRQRHGYGLTPAIVELAAARRPELLITVDNGIASLEGVAAAQARGWQVLVT
                     DHHLPGERLPAADAIVNPNQPGCSFPSKALAGVGVMFYVLLALRARLRTSGWYGACGL
                     TEPNLARLLDLVALGTVADVVPLDHLNRVLVGQGLARIRSGAAVPGIRALAEVAGRDP
                     ARLGCSDIGFTLAPRLNAAGRLEDMALGIECLLCDDPVRARELAVTLDRLNRERRDIE
                     AVMQAAALEDLERLGLDAAGLPFGLCLYDPHWHQGVVGILAARVRERTHRPTIVFAPG
                     DPGWLKGSARSVPGLHLRDALAAVDARHPGLIERFGGHAAAAGLTLAEANYPDFAAAF
                     DAEVRRHLDAGDLRGVLYSDGELAPGDCTLAIAERLRDGGPWGQGFPEPLFDGEFEVV
                     AARTLKERHLK"
BASE COUNT          357 a         1013 c         1089 g          435 t
ORIGIN      
        1 cggcgaacat gtcgcggacg ctgtagatcg ggccggccgg cacgccggcg gtttcgaggc
       61 tcgacacgac ttcggcgagc ggcagcgtgg cggtccatgc ggcgatcgcg gcgtcgatct
      121 cggcttcgtg ctgcacgcgg ccggggttgt tcgcgaggcg tggatcgtcg gccaggtcgg
      181 cacggccggc ggcgcgcatc aggcgcttgt agatggaatc gccgttgccg ccgatgatcg
      241 cccagcggcc gtcggcgcag cggtaggtgt tggtcggcac gatgccggtc accgtcgtgc
      301 ccgagggctc gcgcacgacg ccggcgccgt cgaactcggg gaccacgctt tcgagcaggt
      361 tgtagacggc ctcgaacagc gccacgtcga cgacctgacc gctgccggag cgcccgcgct
      421 ggtgcagcgc catcaggatg ccgatcaccg cgtgcagcgc cgcgagcgtg tcgccgatcg
      481 acaggttcgg gcgcaccggc ggttctccgg gcatgccgtt gacgtagcgc aggccgccga
      541 agccctcgca gaccgaggcg tagccggcct tgccggcgta ggggccgtcc tggccgtagc
      601 cggagatgcg cgcgatcacg aggccggggt tcgcggcctt cagcacctcc ggacccaggc
      661 cccatttctc cagcgtgccg gggcggaaat tctcgacgag cacgtcgcag cgcgcggcga
      721 gctggccgac cagcgcccgg ccctcgccgg tcttcaggtc gatgcggacg ctcttcttgt
      781 tgcgaccgat gctgtgccac cacagcgagg tgccgtcgcg cacgacgcgc cactggcgga
      841 tcgggtcgcc ttcgggcggt tcgatcttga tgacctcggc gccgaagtac gcgagcagcg
      901 tgccggtgaa cgggccggcg atgagctggc cgagttcgag cacgcgcagc ccggcaagcg
      961 ggccgggggc cggacggtca tcggcaggag tctgggtcat ggcggggctt ccgggcgcgg
     1021 gctgcgggac gggtggatgg ccggggagtg tggcgacggc cgccggcgtc tgccagtcgc
     1081 aggcagcagg ctttggcagc gcgctgcgcg cagccgcaac ggtgaccggt gccggggctc
     1141 gccgatgtgc gcccggtgtc gcaggtcttg aatgaccatg gctggttcgt ggttgaattc
     1201 gcagttttgg agcggtgcag ccattacctt gggggcactg cggcgcaagc gtgccaggcg
     1261 ccggctccgt cccccttgcc gcgtccgcag catccggcga tgctgcgccg cgcccgccgc
     1321 gatgagcccg ttctgaatgc cgacagcaat cgtacgccga cccctgccga gccccggcga
     1381 acggccggaa ctgcccgccg acctgccgcc gctgctggcg cgggtctatg cgctgcgcgg
     1441 ggttgccagc gcccgtgagc tcgaccaggc gctgacggcg ctgccggcac cgcaacggct
     1501 gaagggcatc gatcgcgccg tcgacctgct gagcgcggcg ctgagcaccg atgcccgcat
     1561 cctgatcgtc gccgactacg acgccgatgg cgccaccgcc tgcgcgctgg cggtgcgcgg
     1621 gctgcgcgcg ctcggggcgc gccacgtcga ttatctggtg ccgaaccgcc agcgccacgg
     1681 ttacgggctg acgccggcga tcgtcgaact cgcggccgca cggcggccgg agctgctgat
     1741 caccgtcgac aacggcatcg ccagcctcga aggcgtcgcc gcggcgcagg cgcgcggctg
     1801 gcaggtgctg gtcaccgatc accacctgcc cggcgagcgg ctgccggcgg ccgatgccat
     1861 cgtcaatccg aaccagccgg gctgcagctt tccgtcgaag gcgctggccg gcgtcggcgt
     1921 gatgttctac gtgctgctgg cgctgcgcgc gcggctgcgc acgagcggct ggtacggcgc
     1981 ctgcggactg accgagccga acctcgcgcg cctgctcgat ctggtcgcgc tcggcaccgt
     2041 tgccgatgtc gtgccgctcg atcacctcaa ccgcgtgctg gtcgggcagg gtctggcccg
     2101 catccgcagc ggtgccgccg tgcccggcat ccgcgcgctg gccgaggtcg ccggccgcga
     2161 tccggcgcgc ctcggctgtt ccgacatcgg cttcacgctg gcgccgcgcc tgaacgccgc
     2221 cggccggctc gaagacatgg cgctcggcat cgaatgcctg ctctgtgacg atccggtgcg
     2281 cgcgcgcgag ctggccgtga cgctggaccg gctgaaccgc gagcggcgcg atatcgaggc
     2341 cgtgatgcag gcagcggcgc tggaggacct cgaacggctc gggctcgatg cggccgggct
     2401 gccgttcggt ctgtgcctgt acgacccgca ctggcatcag ggcgtggtcg gcatcctcgc
     2461 ggcgcgcgtg cgcgagcgca cgcaccggcc gacgattgtc tttgcgcccg gcgatccggg
     2521 ctggctgaag ggttcggcgc gctcggtgcc gggcctgcac ctgcgcgatg cgctggccgc
     2581 ggtcgatgcg cgccatccgg ggctgatcga gcgcttcggc ggtcacgccg ccgccgccgg
     2641 cctgacgctg gccgaggcga attatccgga cttcgctgcg gctttcgatg ccgaggtacg
     2701 ccgtcacctc gacgccgggg acctgcgcgg cgtgctgtat tccgacggtg aactggcgcc
     2761 gggcgactgc acgcttgcga tcgccgagcg cctgcgcgac ggcgggccgt ggggccaggg
     2821 ctttccggag ccgctgttcg acggcgagtt cgaggtcgtc gccgcccgca cgctgaagga
     2881 gcggcacctg aagc
//
LOCUS       DAOQJH010000366         2891 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_23560_length_2891_cov_3.970733, whole genome shotgun sequence.
ACCESSION   DAOQJH010000366 DAOQJH010000000
VERSION     DAOQJH010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2891)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2891)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2891
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23560_length_2891_cov_3.970733"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="PLQ18_08785"
     CDS             complement(<1..185)
                     /locus_tag="PLQ18_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012316347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="laccase domain-containing protein"
                     /protein_id="HNE01788.1"
                     /translation="MSGEPLALLQPDWPAPANVRAAATTRRGGVSRGPYAALNLGDHV
                     GDDPAAVAENRRLLNLYA"
     gene            complement(182..1195)
                     /gene="rluD"
                     /locus_tag="PLQ18_08790"
     CDS             complement(182..1195)
                     /gene="rluD"
                     /locus_tag="PLQ18_08790"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014576073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HNE01789.1"
                     /translation="MPEFERIDCLIPPECAGQRLDQALAALLPDHSRARLQQWLKDGT
                     LTVDGRPAKPRALVLGGERVSGAYERTEVIADRPQDIPLVVRYEDDDILVIDKPAGLV
                     VHPAAGNPDGTLVNALLHHAPVTAQLPRAGIVHRLDKDTSGLLVVAKSLRAHTALVEQ
                     LQARTMGREYQAIACGVLVSGGLVDVPIARHGTDRKRMAATPGGKPAITHFRVRERFR
                     AHTHLALKLETGRTHQIRVHMAFMHHPLLGDPVYGGRLRLPPGCSDELATALKGFRRQ
                     ALHARRLTLTHPGSGRRLSWSAPTPPDLAALLDVLRRDAGLDGLDARAGVDADGVEWE
                     YVE"
     gene            1304..2110
                     /locus_tag="PLQ18_08795"
     CDS             1304..2110
                     /locus_tag="PLQ18_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HNE01790.1"
                     /translation="MSARFLRLRLATVLAAALLAPGCSWLSEKADETRDWSAAKLYSE
                     AKDALNNGDYTRAIGYYDKLQARYPFGRYAQQAQLESVYAYYRDGEPDSAVAAADRFI
                     KTYPRHPYVDYVYYLKGLTNYNRASGPLDRLMPPDPTRTDASAALQSFTDFNDLVTKF
                     PDSRYAPDARARMLFLRNNLASYELNVARHYQSRGAHVAAVNRAKYVVENYASSPAAT
                     EALVIMVRSYTAMGLPELARDALRVLQKNDPQNPDIPKLTTLVGTAPGKG"
     gene            2129..2650
                     /locus_tag="PLQ18_08800"
     CDS             2129..2650
                     /locus_tag="PLQ18_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNE01791.1"
                     /translation="MWDFFETVRHRHSVRIYQQDMPVDEARLHAILEMACAAPSAGDL
                     QAYRIVVVRDADERRALSVAADNQGFIAEAPVCLVFCADPVRSGAEFGERGERLYALQ
                     DTTIAAAYAQLAIVAAGMGSAWIGYFDDQAVRDLLGLDPALQPVALMTVGYPAELPVP
                     TPRRRLAEVVSFR"
     gene            complement(2674..>2891)
                     /locus_tag="PLQ18_08805"
     CDS             complement(2674..>2891)
                     /locus_tag="PLQ18_08805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HNE01792.1"
                     /translation="AARCQSCHGADAHGKDGRARLAGQKPGYLLRVLNDFRQGHDRSP
                     DSVMRSMVQGLSEADVQALASFLGAQP"
BASE COUNT          413 a         1029 c         1031 g          418 t
ORIGIN      
        1 gcgtagaggt tgagcaggcg ccggttctcg gcgaccgcgg ccggatcgtc gccgacgtga
       61 tcaccgaggt tgagggcggc gtacgggccg cggctgaccc cgccgcggcg cgtcgtcgcc
      121 gcggcgcgca cgttcgccgg cgccggccag tccggctgca gcagggcaag cggttccccg
      181 ctcattcgac gtactcccat tcgacgccat cggcatcgac gccggcgcgt gcatcgagcc
      241 cgtcgaggcc ggcgtcgcgg cgcagcacgt cgagcagcgc ggcgagatcg ggcggcgtcg
      301 gcgcgctcca gctcaggcgc cggccgctgc ccggatgcgt cagcgtcagc cggcgcgcgt
      361 gcagggcctg gcgccggaag cccttcagcg ccgtcgcgag ttcgtcgctg cagcccggcg
      421 gcaggcgcag ccggccgccg tagaccggat cgccgagcag cgggtggtgc atgaaggcca
      481 tgtgcacgcg gatctggtgc gtgcggccgg tttcgagctt cagcgccaga tgcgtgtggg
      541 cgcggaagcg ctcgcgcacg cggaaatgcg tgatcgccgg cttgccgccg ggcgtggcgg
      601 ccatgcgctt gcggtcggtg ccgtggcggg cgatcggcac gtcgacgagc ccgcccgaga
      661 ccagcacgcc acaggcgatc gcctggtact cgcggcccat cgtgcgcgcc tgcaactgct
      721 cgaccagggc ggtatgcgcg cgcaggctct tggcgacgac gagcagcccc gaggtgtcct
      781 tgtcgagccg gtgcacgatg ccggcgcgcg gcagctgcgc ggtgaccggc gcgtggtgca
      841 gcagcgcgtt gaccagcgtg ccgtcggggt tgccggcggc cggatgcacg accagcccgg
      901 cgggcttgtc gatgaccagg atgtcgtcgt cctcgtagcg cacgaccagc gggatgtcct
      961 gcgggcggtc ggcgatgact tcggtgcgct cgtacgcgcc gctgacgcgc tcgccgccga
     1021 gcaccagcgc ccgcggcttc gcgggcctgc cgtcgacggt cagcgtgccg tccttcagcc
     1081 actgctgcag gcgggcgcgg gagtgatccg gcagcagcgc ggcgagcgcc tggtcgaggc
     1141 gctggccggc acactccggc gggatcaggc agtcgatgcg ttcaaattcg ggcatggaac
     1201 ggctcggaca gggggcgaga ggcggcccgg ggtgcaggca tggtcttcgt tcgggggcgc
     1261 aagatatact cggccgcccc gtcacctcgt acagatcagc cgaatgtccg ctcgcttcct
     1321 gcgtcttcgc ctcgcgaccg tcctggccgc tgccctgctt gcgcccggct gttcgtggct
     1381 gtcggagaaa gccgacgaga cccgcgactg gtcggcggcg aaactgtaca gcgaggccaa
     1441 ggacgcgctg aacaacggcg attacaccag ggccatcggc tactacgaca agctgcaggc
     1501 acgctacccg ttcgggcgct acgcgcagca ggcgcagctc gaatcggtct acgcatacta
     1561 ccgggacggc gaacccgatt cagcggtggc cgcggccgat cgcttcatca agacctatcc
     1621 gcgccacccc tacgtcgatt acgtctacta cctgaagggc ctgaccaact acaaccgcgc
     1681 cagcggtccg ctcgaccgcc tgatgccgcc ggacccgacg cgcaccgatg cctcggcggc
     1741 gctgcagtcc ttcaccgact tcaacgacct cgtcaccaaa ttcccggaca gccgctacgc
     1801 gccggatgcg cgcgcgcgca tgctgttcct tcgcaacaac ctcgccagct acgaactcaa
     1861 cgtcgcgcgc cactaccaga gccgcggcgc acacgtcgcc gccgtcaacc gcgccaagta
     1921 cgtggtcgag aactacgcga gcagcccggc ggcaaccgag gcgctggtga tcatggtgcg
     1981 ctcgtacacg gccatgggcc tgcccgagct ggcgcgtgat gcgctgcgcg tgctgcagaa
     2041 gaacgatccg cagaatccgg acatcccgaa gctgacgacg ctggtcggca cggcgccggg
     2101 caagggctga cagagaggcg cgccgcgcat gtgggatttc ttcgagaccg tccgtcaccg
     2161 tcactcggtg cgcatctacc agcaggacat gccggtcgac gaggcccgcc tgcacgcgat
     2221 cctggagatg gcctgcgccg ctccgtcggc cggtgacctg caggcctacc gcatcgtcgt
     2281 cgtgcgcgac gccgatgagc gccgggcgct gtcggtcgcg gccgacaatc agggcttcat
     2341 cgccgaggcg ccggtatgtc tggtgttctg cgccgatccg gtgcgctcgg gtgccgagtt
     2401 cggcgaacgc ggcgaacgcc tgtacgcgct gcaggacacg acgatcgccg ccgcctacgc
     2461 gcagcttgcg atcgtcgctg ccggcatggg ctcggcgtgg atcggctact tcgacgatca
     2521 ggccgtgcgc gacctgctcg gcctcgaccc ggcgctgcag ccggtggcgc tgatgacggt
     2581 cggctacccg gccgaactgc cggtgccgac gccgcgccgg cggctcgcgg aagtcgtcag
     2641 tttccgctga ggccgccggc ggcggcgcgc gcctcagggc tgcgcgccga ggaagctcgc
     2701 gagcgcctgc acgtcggcct cggacagacc ctgcaccatc gagcgcatca ccgaatccgg
     2761 cgagcggtcg tggccctggc ggaagtcgtt cagcacgcgc agcagatagc ccggcttctg
     2821 gcccgcgagc cgcgcccggc cgtccttgcc gtgcgcatcg gcgccgtggc agctctgaca
     2881 gcgcgccgcg t
//
LOCUS       DAOQJH010000367         2887 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_23615_length_2887_cov_3.417726, whole genome shotgun sequence.
ACCESSION   DAOQJH010000367 DAOQJH010000000
VERSION     DAOQJH010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2887)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2887)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2887
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23615_length_2887_cov_3.417726"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..725
                     /locus_tag="PLQ18_08810"
     CDS             <1..725
                     /locus_tag="PLQ18_08810"
                     /inference="COORDINATES: protein motif:HMM:NF022812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3160 domain-containing protein"
                     /protein_id="HNE01793.1"
                     /translation="RYEGYAEAWGRAREQLDADADESGPAAARLTLLRAGFDAAAGAP
                     AARERLTAMLGFWTYQRRLDQLYVKQGYTLAGKGLELPSPGRQRSGAMIEVEALPLLR
                     ALLGFVEHEQAVAAHPGRLAFRELLTHCIGIVERLQAGSSVDAQDEDFLNRLDQALLG
                     LAGEGDAPVIVDLHTDAASAEVLELATGPAREIRHGPATGARISVHQFRQPLSGRMTD
                     ADWRARLHSGEVPPPIDVGAGV"
     gene            722..2443
                     /locus_tag="PLQ18_08815"
     CDS             722..2443
                     /locus_tag="PLQ18_08815"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HNE01794.1"
                     /translation="MNPEPDRPLRRLWLLGVLLLVFGMDAGARNLALLVGINQYAGVQ
                     PLEGAVNDATELRSVLIERWGFAAQDIVTLLDVQATRAAILHELDRLLIRSRPGDLVL
                     VYFSGHGTSGRDRGFGLPLPYSTGAFVPVEFKAEGTREEMISRLLVGRTDLRPRIEAL
                     DRGGRQVLALIDACYSGNTLRGLYRNTLARPAFTKRYVALPPRGAGSEPAAKDPDLCG
                     YNCASAVEEAFPYRAVAYVSSASEGEAAVDLGSSVLASVPTYDGRPHGAFSDALLRAL
                     RGEGPLLADRDGDGRLSAAELHQVTLNFLRQRGLPHTPQLLPRLEDDRSGIAAAIFLQ
                     GTPPPGTVPAAPAAGRTLRVQLVDATPALTTVLQGLAQLTLVERDARLIVRRDGEEWQ
                     LQSAAGDEITRLPATGIKKVVAAVRAQAWVERVLAAPQDSGFRLELELSGQARGSVAV
                     GGQSIHFAVRSAQPAQLLVLTVSTDGSVAVLYPGNAAELQPVPAGTAVRIPSVDTESI
                     LVQAPFGTDQVLAFAFPHPEPRLRELIGGFWESGAPQLEALGDWLGDPALARAQLSLV
                     TRPGPGS"
     gene            2464..>2887
                     /locus_tag="PLQ18_08820"
     CDS             2464..>2887
                     /locus_tag="PLQ18_08820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE01795.1"
                     /translation="MTEFQSGSMKRVVAVLVGLGSLSLSGAFAEDCQRGEQMFEQSKT
                     EPQASQRIVLLERSASLCGRFATHYALGRERLALGVTGPAAEAFETALQLAGDDRAAM
                     LALGRLAEARYEQGRIGEAVAALEGAYGQLAAVSPPQAA"
BASE COUNT          419 a          939 c         1028 g          501 t
ORIGIN      
        1 atcgctatga aggatatgcc gaagcctggg gccgggcgcg cgaacaactg gatgccgatg
       61 ccgacgaatc ggggccggcg gcggcacgtc tgaccctgct gcgtgccggt ttcgatgcgg
      121 ctgccggcgc cccggcggcc cgggagcggt tgacggcgat gctcgggttc tggacctacc
      181 agcgccgcct cgatcaactc tacgtcaagc aggggtacac gctggccggc aaggggctgg
      241 aactgccatc gccgggccgg cagcgcagcg gagccatgat cgaagtcgag gccctgccgc
      301 tgttgcgggc gctgctcggc ttcgtcgaac acgaacaggc cgtcgccgca cacccggggc
      361 ggctggcgtt ccgggaactg ctcacgcatt gcatcggtat cgtcgagcgt ctgcaggccg
      421 gcagcagcgt tgatgcgcag gacgaggatt tcctgaaccg cctcgatcag gcgctgctcg
      481 ggcttgccgg cgagggggat gcaccggtca tcgtcgatct gcataccgat gcggccagcg
      541 ccgaggtgct ggaactggcg accggtccgg cgcgtgaaat ccggcacggt ccggccaccg
      601 gggcccggat ttcggtgcat cagttccgcc agccgctgtc ggggcgcatg actgacgccg
      661 actggcgcgc gcgcctgcac agcggtgagg taccaccacc gattgatgtc ggtgccggcg
      721 tgtgaatcct gagccggatc ggccgctacg acggctgtgg ctgctcgggg tgctgctgtt
      781 ggtgttcggc atggatgccg gggcgcgcaa cctggccttg ctggtcggca tcaaccagta
      841 cgctggggtc cagcccctcg aaggggccgt caacgatgcc acggaactgc gttcggtcct
      901 gatcgaacgc tggggattcg cagcgcagga catcgtcacg ctgctggacg tgcaggccac
      961 ccgggcggcg atcctgcacg aactcgaccg gctgctgatt cgcagccgcc ccggggatct
     1021 ggtgctcgtg tacttcagcg gtcacggcac cagcggtcgc gaccggggtt tcggtctgcc
     1081 gctgccgtat tcgaccggcg cattcgtacc ggtcgaattc aaagcggagg gcacgcgcga
     1141 ggaaatgatc tcgcgcctgc tggtcgggcg cacggacctg cgcccgcgca tcgaggcgct
     1201 ggatcgtggc ggccggcagg tgctggcgct gatcgatgcc tgttattcgg gcaatacctt
     1261 gcgcggcctg taccgcaata cgctggcacg gccagccttt accaagcgtt atgttgcgtt
     1321 gccgccccgc ggtgccggca gcgaaccggc ggcgaaggac ccggacctct gcgggtacaa
     1381 ctgcgccagt gctgttgaag aagcctttcc gtaccgggct gtggcctacg tatcctcggc
     1441 cagcgaaggc gaggcggcgg tggatctcgg ctccagcgtg ctggccagcg tgcctaccta
     1501 tgacggccgc ccgcacggag ccttcagcga tgccttgctg cgcgccctgc ggggggaggg
     1561 accattgctc gccgaccggg acggggatgg ccggctcagt gctgccgaac tgcatcaggt
     1621 gacgctgaat ttcctgcgcc agcgcggcct gccgcatacc ccgcagctgt tgccgcgcct
     1681 cgaagacgat cggagcggca ttgccgctgc cattttcctg cagggaacgc cacctccggg
     1741 tacggtgccc gctgcgcctg cggccggccg caccctgcgg gtccagctcg tcgacgcgac
     1801 gccggccctg accacggttc ttcagggact ggcgcaactg acactcgtcg aacgcgatgc
     1861 ccgcttgatc gtgcgccgtg atggagagga gtggcagctg cagtcagcgg ccggcgacga
     1921 gattacccgg ctgccggcaa cgggcatcaa gaaggtcgtg gcagccgtgc gggcacaggc
     1981 ctgggtcgag cgcgtgctgg cagcgccgca ggattcgggg ttccggctcg aactcgagct
     2041 ttccggtcag gctcgcggca gtgtggccgt cggcgggcag tcgatccatt tcgcggtacg
     2101 cagcgcgcag cccgcgcaac tgctggtact gaccgtatcc accgacggca gcgtcgcggt
     2161 gttgtatccg ggcaatgccg ccgaactgca gcccgtgccg gccgggacgg ccgtgcgcat
     2221 tccgtcggtc gataccgaat ccatcctggt acaggcgccg ttcggcactg atcaggtgct
     2281 ggcctttgcg ttcccgcacc ccgagccgcg attgcgggag ctgatcggcg gtttctggga
     2341 gtccggtgca ccgcagctgg aagccctggg cgactggctc ggtgatccgg ccctggcccg
     2401 tgcgcagctg tcgctggtga cccgtcccgg gcccggatcc tgaaccttct gccggaggca
     2461 gccatgaccg aattccagtc cggttccatg aagcgggtgg tggccgtgct tgtcgggctg
     2521 ggcagtctga gtctttcagg ggcttttgcc gaagactgtc agcgtggcga acaaatgttc
     2581 gagcaatcga agaccgagcc gcaggcgtcg cagcggattg tgctgctgga gcgttcagcg
     2641 tccctgtgtg gccgctttgc cactcattat gccctcgggc gtgaacggct ggcgctcggt
     2701 gtcaccggac cggcagccga agccttcgag accgccctgc agctggccgg cgatgaccgc
     2761 gctgcaatgc tggctctcgg gcgactcgcc gaggcccgtt acgaacaggg ccggattggt
     2821 gaggcggtgg cggcgctcga aggtgcttat ggccagctgg cagctgtctc gccgccgcag
     2881 gctgctc
//
LOCUS       DAOQJH010000368         2883 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_23670_length_2883_cov_4.039958, whole genome shotgun sequence.
ACCESSION   DAOQJH010000368 DAOQJH010000000
VERSION     DAOQJH010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2883
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23670_length_2883_cov_4.039958"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(81..1316)
                     /locus_tag="PLQ18_08825"
     CDS             complement(81..1316)
                     /locus_tag="PLQ18_08825"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNE01796.1"
                     /translation="MNSSSVSEAVARTDATELETLRAEVARYRHWMSEVTRVCRSAAD
                     GDLEPRLIHIDAEGDLAMMLHSINRMLDMSDALLRESSATLDYASHDKYFRPILPHGL
                     RGSFKRTAEQINGAIGEMQRKSISLHTAEDRRQKLAHSFEASIRGVAQTLAAAVTELA
                     STAESLKRIADLTVDRATSAAAASTQTAERMSRIAESTHGLAESIGDIGRRVSASTEL
                     AHQTAQDVQRADGTVRELAGCTSSIGRVSGLIQKIAGQTRLLALNATIEAVRAGSAGR
                     SFAVVASEVKALSNQTAGATDDIGNQINRIQGATDEVVRSIADITRSITDIDRNSSSI
                     SSTVQEQMRATDDISSAVRAAADGSRAVSADLQEMMEAATGTTSAADELLVAARELSH
                     MAEQLNAEVDDFVHHLYSS"
     gene            complement(1294..1818)
                     /locus_tag="PLQ18_08830"
     CDS             complement(1294..1818)
                     /locus_tag="PLQ18_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011386362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNE01797.1"
                     /translation="MAKYKVQPSGRERFFPENDIIVSKTDLKGHITYANRVFLDIAGL
                     GEDEALGEPHCLIRHPDMPRCVFRLLWDTIAGGSECFAYVKNMAVNGDHYWVLAHVTP
                     TFGPGGDIIGYHSNRRVPRRDAVTKAEALYRTLLAEEQRHTDRSAGLAASYALMQRLL
                     AEQGVAYDEFVLGL"
     gene            2140..2388
                     /locus_tag="PLQ18_08835"
     CDS             2140..2388
                     /locus_tag="PLQ18_08835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE01798.1"
                     /translation="MKITLTHIPETADIDAVRAFLREHGIDDAGEIVLIEGDGSLPAV
                     AFELEVSAAVAESIAAHLRGKLWDGHVIGAGLLLMSGH"
     gene            2502..>2883
                     /locus_tag="PLQ18_08840"
     CDS             2502..>2883
                     /locus_tag="PLQ18_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013106968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3141 domain-containing protein"
                     /protein_id="HNE01799.1"
                     /translation="MSQTNPNLGQPAPAGLPERLQALADRWSDWQDELGGVLGHDLQR
                     VVAAAAARQSRRCDQAARLATAAWQPGQTAGVLHYAVDSLQRTLLFWDTLRERGNDFI
                     ELYRAGNPPLLAFDYETLIDGRNFD"
BASE COUNT          396 a          970 c         1019 g          498 t
ORIGIN      
        1 aagcggagcg cgacgacgcc cccgaaacat taccgcatgg taacgcggcg ctgcccgggg
       61 gcgttacgga agtcgccggc tcagctgctg tacaggtgat gcacgaagtc gtcgacctcg
      121 gcgttgagct gctcggccat gtgcgacagc tcgcgcgccg ccaccagcag ttcgtcggcg
      181 gccgaggtcg tgccggtcgc ggcttccatc atctcctgca gatcggccga caccgcgcgc
      241 gagccgtcgg cggcggcacg cacggcggag ctgatgtcat cggtcgcgcg catctgctcc
      301 tgcaccgtgc tcgagatcga cgaggagttg cggtcgatgt cggtgatcga gcgcgtgatg
      361 tcggcgatcg agcgcacgac ctcgtcggtc gcgccctgga tgcggttgat ctggttgccg
      421 atgtcgtcgg tggccccggc ggtctggttc gacagcgcct tcacctccga ggcgaccacc
      481 gcgaagctgc gcccggcgct gccggcgcgc acggcctcga tcgtcgcgtt cagcgccagc
      541 agccgcgtct ggccggcgat cttctggatc aggccgctga cgcggccgat gctgctggtg
      601 cagccggcga gttcgcgcac ggtgccgtcg gcgcgctgca cgtcctgcgc ggtctggtgc
      661 gcgagttcgg tcgaggcgct gacgcgccgg ccgatgtcgc cgatcgattc ggccagaccg
      721 tgcgtggact cggcgatgcg gctcatgcgc tcggcggtct gcgtcgaggc cgccgcggcg
      781 ctggtggcgc gatcgacggt caggtcggcg atgcgcttga gcgattcggc ggtcgaggca
      841 agctcggtga ccgccgccgc cagcgtctgc gcgacgccgc ggatcgaggc ctcgaaggaa
      901 tgcgcgagct tctggcggcg atcctcggcc gtgtgcagcg agatgctctt gcgctgcatc
      961 tcgccgatcg cgccgttgat ctgttcggcc gtgcgcttga agctgccgcg caggccgtgc
     1021 ggcaggatcg gccggaagta tttgtcgtgg ctcgcgtaat cgagtgtcgc actcgattcg
     1081 cgcagcagcg cgtcgctcat gtcgagcatg cggttgatgc tgtgcagcat catggcgagg
     1141 tcgccttcgg cgtcgatgtg gatcagccgc ggttcgagat ccccgtcggc cgccgagcgg
     1201 cagacgcgcg tgacctcgct catccagtgc cggtagcgcg cgacttcggc acgcagcgtc
     1261 tccagctcgg tggcgtccgt gcgcgcgacc gcttcagaga ccgaggacga attcatcgta
     1321 ggcgactccc tgttcggcga gcaggcgctg catcagcgca tacgaggcgg cgagaccggc
     1381 actgcggtcg gtgtggcgct gctcttcggc gagcagcgtg cggtacagcg cttcggcctt
     1441 ggtgacggcg tcgcgccgcg gcacgcgccg gttggagtgg tagccgatga tgtcgccgcc
     1501 ggggccgaag gtcggcgtca cgtgggcgag cacccagtag tgatcgccgt tgacggccat
     1561 gttcttgacg taggcgaagc attcgctgcc gccggcgatg gtgtcccaca gcagccggaa
     1621 cacgcagcgc ggcatgtcgg ggtggcggat caggcagtgc ggttcgccca gggcctcgtc
     1681 ctcgccgaga ccggcgatgt cgaggaacac gcggtttgcg taggtgatgt gccctttcag
     1741 gtcggttttc gaaacgatga tgtcgttttc agggaaaaac cgttcacgcc cgctgggctg
     1801 gaccttgtac ttcgccatcg ctctctcccc cgcgtccgcg gcatcggcgg cgccggatgt
     1861 tggtttggtg agccagcaga ccgctatagg aagcgcggcc ggtgcgcggc cagccggcag
     1921 tctatgcaat gcctccatgc cccgcccggt gccgacggca tcgcggcagc ggtcgacacg
     1981 cgagttcact gccccatatc ggccgcttgc gcgcgggctg cagggcaggg cagtttttcg
     2041 ctgcactgct aggctgcgtg aggtccgcgc cggtgtttgc aggtcctgcc gggaccgctt
     2101 ccgtgcgctg ccgaacccgt cctgcaggga aaccgcctga tgaagatcac cctgacccac
     2161 atccccgaaa ccgccgacat cgatgccgtg cgcgccttcc tgcgcgagca cggcatcgac
     2221 gatgccggcg agatcgtgct gatcgagggc gacggcagtc tgccggccgt ggccttcgag
     2281 ctcgaggtca gtgccgcggt cgccgagtcc atcgccgcgc atctgcgcgg caagctctgg
     2341 gacggccacg tcatcggcgc cggcctgctg ctgatgtccg gtcactgacg gccggcctgc
     2401 accgcacgag ccgcagcatt gctgcgaaat cctgccttca tcgcactgca ccggcaggcc
     2461 ggcgcggtgc ttctcctgtc atctcccgtg agggtcagct catgagtcag acgaatccga
     2521 acctcgggca gccggcgccg gccggactgc cggaacgcct gcaggcgctc gccgaccgct
     2581 ggtcggactg gcaggacgaa cttggtggcg tgctcggcca tgacctgcag cgcgtcgtgg
     2641 ccgccgccgc cgcgcgtcag tcgcgccgct gcgatcaggc cgcacgtctc gcaaccgcgg
     2701 catggcagcc ggggcagacg gcaggcgtcc tgcactacgc cgtcgactcg ctgcagcgca
     2761 cgctgctgtt ctgggacacg ctgcgcgagc gcggcaacga cttcatcgag ctgtaccgtg
     2821 ccggcaatcc gccgctgctc gccttcgact acgagaccct gatcgacggg cgcaacttcg
     2881 acg
//
LOCUS       DAOQJH010000369         2880 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_23705_length_2880_cov_4.896991, whole genome shotgun sequence.
ACCESSION   DAOQJH010000369 DAOQJH010000000
VERSION     DAOQJH010000369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2880)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2880)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2880
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23705_length_2880_cov_4.896991"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..174)
                     /locus_tag="PLQ18_08845"
     CDS             complement(<1..174)
                     /locus_tag="PLQ18_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013593726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HNE01800.1"
                     /translation="MEGLADALLRAALTSVGGIDGCVTEFVRVTDTLLPRAVWLRRVP
                     ELAHGGRTPAGVPV"
     gene            complement(213..608)
                     /gene="gloA"
                     /locus_tag="PLQ18_08850"
     CDS             complement(213..608)
                     /gene="gloA"
                     /locus_tag="PLQ18_08850"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004462 - lactoylglutathione lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0051596 - methylglyoxal catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HNE01801.1"
                     /translation="MRYLHTMIRTGDLDRSIAFYRDVLGFRLVSRTDYPDGEFTLAFL
                     QAPGDDGNDGPMLELTYNWGVSSYEPGKGYGHVAFKVESMDAFAENLRARGHDFSWGP
                     GKAPNGRTAIAFIVDPDGYQIELIERADA"
     gene            complement(653..853)
                     /locus_tag="PLQ18_08855"
     CDS             complement(653..853)
                     /locus_tag="PLQ18_08855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE01802.1"
                     /translation="MVDAVLLREKLVQRRLPVFTIIDRRLVRMPQSITGRPAHTPGPA
                     VASLAGQRAELLAALELLFTGI"
     gene            complement(975..1220)
                     /locus_tag="PLQ18_08860"
     CDS             complement(975..1220)
                     /locus_tag="PLQ18_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019584372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system CcdA family
                     antitoxin"
                     /protein_id="HNE01803.1"
                     /translation="MAAVFDPDAPRTATGLGVNTGLLRQVREFGVPLDDSCERALAEA
                     VRAARMRAWQEENRPWIEAYNAWIAEHGCFGEEYRSF"
     gene            complement(1289..2494)
                     /locus_tag="PLQ18_08865"
     CDS             complement(1289..2494)
                     /locus_tag="PLQ18_08865"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011063107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HNE01804.1"
                     /translation="MSEINKVVLAYSGGLDTSVILKWLQDTYQCEVVTFTADIGQGEE
                     LEPARAKALAMGVQQIYIDDLREEFVRDFVFPMFRANTLYEGEYLLGTSIARPLIAKR
                     LIEIANETGADAISHGATGKGNDQVRFELGAYALKPGVKVIAPWREWDLTSRETLLDY
                     AEKHGIPVDRYGKKSPYSMDANLLHISYEGQVLENPWAEAEEDMWRWTVAPEAAPDKA
                     TYLELTFRAGDVTAIDGVDMSPATVLATLNRIGGDNGIGRLDLVENRYVGMKSRGCYE
                     TPGGTILLKAHRAIESLCLDREVAHLKDELMPRYASIIYNGYWWSPERRMLQAMIDAS
                     QAPVNGVVRLKLYKGNVMVAGRKSDDSLFDPTIATFEDDGGAYNQKDAEGFIKLNALR
                     MRIAANKGR"
     gene            complement(2629..>2880)
                     /locus_tag="PLQ18_08870"
     CDS             complement(2629..>2880)
                     /locus_tag="PLQ18_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012351389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HNE01805.1"
                     /translation="VERACGVCTTVHALASVRQNEVTKALAGWAAILAVPTVVFGLYG
                     MNFDKMPELHWHYGYPAIVVATFLVCVWLYLRLKRTGWL"
BASE COUNT          467 a          967 c          987 g          459 t
ORIGIN      
        1 caccggcacg cccgcgggcg tgcgcccgcc gtgcgcgagt tccggcacgc gccgcaacca
       61 caccgcgcgc ggcagcagcg tgtcggtgac gcgcacgaac tcggtcacgc agccgtcgat
      121 gccgccgacc gaagtcagcg cggcgcgcag cagcgcatcg gcgagcccct ccatcggcgc
      181 cagcagcagc cggccaccgg cggcgacggc cgtcaagcgt cggcgcgctc gatcagctcg
      241 atctggtagc cgtccggatc gacgatgaag gcgatcgcgg tgcggccgtt cggggccttg
      301 cccgggcccc agctgaagtc gtggccgcgg gcacgcaggt tttcagcgaa ggcgtccatc
      361 gactcgacct tgaacgcgac gtggccgtag cccttgccgg gttcgtagga cgacacgccc
      421 cagttgtagg tcagctccag catcggccca tcgttgccgt catcgcccgg ggcctggagg
      481 aaggcgagcg tgaattcgcc gtccgggtaa tcggtgcgcg acaccagccg gaaaccgagc
      541 acgtcacggt agaaggcgat cgagcggtca agatcgccgg tgcggatcat cgtgtgcagg
      601 tagcgcatgg cagatgccct cgggggtacg ccggtcaggg gaaggaccgg attcagatgc
      661 cggtgaacag cagctcgagt gcggcgagca gttccgcgcg ctggccggcc agcgaggcga
      721 cggcgggacc gggcgtgtgc gccggccggc cggtgatcga ttgcggcatc cgcaccagtc
      781 gtcgatcgat gatcgtgaag accggcagcc gccgctgcac gagcttctcg cgcaagagca
      841 ccgcatcgac caacgatgac gcgcgactcg aagacggcga tcagatcgtg ctgcacgtcg
      901 aggaggtacg gcacccgcgt ggccgtgccg gcattcggat tgcggcagac atcgaactgc
      961 gccatcgccg tgactcagaa gctccggtat tcctcgccga aacagccatg ctcggcaatc
     1021 caggcgttgt atgcctcgat ccacggacgg ttctcttcct gccacgcccg catgcgcgcc
     1081 gcgcgcacgg cctcggccag tgcgcgttcg cagctatcgt cgagcgggac gccgaactcg
     1141 cggacctggc gcagcaggcc ggtgttgacg cccagtccgg tcgcggtacg cggggcatcg
     1201 gggtcgaaga cggcggccat cgggggattc ctcggcgcag gcaggagatt ctgccggcga
     1261 gtatgacccg aaagccgccg ggcgcgactc agcggccctt gttggcagcg atgcgcatgc
     1321 gcagggcgtt gagcttgatg aagccctcgg cgtccttctg gttgtaggcg ccgccgtcgt
     1381 cctcgaaggt cgcgatggtc gggtcgaaca gcgaatcgtc cgacttgcga ccggcgacca
     1441 tgacgttgcc cttgtagagc ttcaggcgca cgacgccgtt caccggggcc tgcgaggcat
     1501 cgatcatggc ctgcagcatc cgccgctccg gcgaccacca gtagccgttg tagatgatgc
     1561 tcgcgtagcg cggcatcagt tcgtccttca ggtgcgcgac ctcgcgatcg aggcacagcg
     1621 actcgatcgc gcgatgcgcc ttcagcagga tcgtgccgcc gggggtttcg tagcagccgc
     1681 gcgacttcat gccgacgtag cggttctcga ccagatccag ccggccgatg ccgttgtcgc
     1741 cgccgatgcg gttcagcgtc gcgagcacgg tcgccggact catatcgacg ccgtcgatcg
     1801 cggtcacgtc gccggcgcgg aaggtgagtt cgagataggt ggccttgtcc ggcgcggcct
     1861 cgggcgcgac cgtccagcgc cacatgtcct cctcggcctc ggcccagggg ttttcgagca
     1921 cctggccttc gtaggagatg tgcagcaggt tggcgtccat cgagtacggc gacttcttgc
     1981 cgtagcgatc gaccgggatg ccgtgctttt cggcgtagtc gagcagcgtc tcgcgcgagg
     2041 tcaggtccca ttcgcgccac ggcgcgatga ccttgacgcc gggcttcagc gcgtaggcgc
     2101 caagctcgaa gcgcacctgg tcgttgccct tgccggtcgc gccgtgcgag atcgcatcgg
     2161 cgccggtctc gttggcgatc tcgatcaggc gcttggcgat cagcggccgg gcgatcgagg
     2221 tgccgagcag gtactcgcct tcatagaggg tgttggcgcg gaacatcggg aacacgaagt
     2281 cgcgcacgaa ctcctcgcgc agatcgtcga tgtagatctg ctgcacgccc atcgccagcg
     2341 ccttggcgcg tgccggttcg agctcctcgc cctgaccgat gtcggcggtg aaggtcacca
     2401 cctcgcactg ataggtgtcc tgcagccact tcaggatcac cgaggtgtcg agaccgccgg
     2461 aataggccag cacgaccttg ttgatttcgg acatgaactt ccccagcgcg cgtcggcggc
     2521 ggatacggat ggaacggtcg gccctgcacg ccagtcgcga tgccggccgt ggaaacgcgc
     2581 cagcttacgc gccggtcacg gccggtcaag gcagccggca agcccgcttc acagccagcc
     2641 ggtgcgcttg agccgcaggt acagccacac gcacaccaga aaggtcgcca cgacgatcgc
     2701 cggatagccg tagtgccagt gcagctccgg catcttgtcg aagttcatgc cgtacaggcc
     2761 gaacaccacg gtcggcaccg ccaggatcgc cgcccagccg gccagcgcct tcgtgacctc
     2821 gttctggcgc accgaggcga gcgcatgcac cgtggtgcag acgccgcagg cgcgttccac
//
LOCUS       DAOQJH010000370         2877 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_23752_length_2877_cov_5.051736, whole genome shotgun sequence.
ACCESSION   DAOQJH010000370 DAOQJH010000000
VERSION     DAOQJH010000370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2877)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2877)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2877
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23752_length_2877_cov_5.051736"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1399
                     /locus_tag="PLQ18_08875"
     CDS             <1..1399
                     /locus_tag="PLQ18_08875"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNE01806.1"
                     /translation="VNGVLVIDYDAAPIRESARRSAALLLLAGSVVIGLTLATLWLLL
                     RRHVIRPLGELDQATRALAAGDLGVRAPVGEDDEIGRAAASFNRMAGRLEQQMALVRA
                     HEAYLQEILDGLPDGVRVIRASDKRVVLANRAFCQQVGRTQSEILGAACHRSSHGRAE
                     PCAPTLVVCPLAELREVGATLKATHHHLRADGTQFPAEVSAVLVEIAGDAGPERYVVE
                     SIRDLGQAVRISHEQRLSELGLLAAGIAHEIHNPLGSVRLGVQGLTREIHEGRIRPEQ
                     IAEYMGLIDQEIDNCIAVTRRLLLLARPPDRSVQLVVLGDALRDTLRLLEFDAQARGI
                     RQRVEVPAEELRVFADEAEIRMIFLNLLQNAHHAMPDGGDLTARLARDGDHAVIEIAD
                     TGVGMEPEHASRIFDPFYSRRADGVSGTGLGLTIVRNFIQRTGGEVGVDSAPGKGTRF
                     TIRLLLAEHSPGVPS"
     gene            1396..2727
                     /locus_tag="PLQ18_08880"
     CDS             1396..2727
                     /locus_tag="PLQ18_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020559842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNE01807.1"
                     /translation="MKRKTLLIIDDDAIFNGLLRRQIESMGFAAVGAGSWAEAQQKLG
                     EIEPDAIILDFKLPDSDATRVLGEIRNQYPVIVLTGFGSIPNAVSAIREGAADFLTKP
                     VNVDELEMTVRRVLETAELRESNQFYRMQLAAHRPGPLVFDSAAMREVQAMIDAVGPT
                     DTTVLILGESGTGKELVAQAIHDRSARAQRELVAIDGSGLQESLFESELFGHERGAFT
                     GADRQKKGLIEEATGSTLFLDEVGDVGAAIQAKLLRVMETGTFRRLGGNKTLTADARF
                     VAATNRDIEAMSRDGRFRSDLYFRLSRFVITIPPLRERREDVEPLARHFLALLTRTAP
                     MSIAPEAMKVLLGYDWPGNVRELRNAVERAIILARPGSQVRAEHLSFIPRALAGETVL
                     RFAGEPTLEQIEREYLRQVLSRLGGNRQKTAAVLGISERNVYRLIEKHRTA"
     gene            2801..2876
                     /locus_tag="PLQ18_08885"
     tRNA            2801..2876
                     /locus_tag="PLQ18_08885"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2834..2836,aa:His,seq:gtg)
BASE COUNT          470 a          999 c          977 g          431 t
ORIGIN      
        1 cgtgaatggc gtgctggtga tcgactacga cgcggcgccg atccgcgaga gcgcacgacg
       61 cagcgcggcg ctcctgctcc tcgccggcag cgtggtgatc ggactgacgc tggcgacgct
      121 gtggctgctg ctgcgtcgcc acgtcattcg ccccctcgga gagctcgacc aggccacccg
      181 ggcgttggcc gccggcgacc tcggcgtgcg tgcaccggtc ggcgaggatg acgaaatcgg
      241 ccgcgcggcg gcgagcttca accgcatggc cggccgcctt gagcagcaga tggccctggt
      301 gcgcgcccat gaagcctatc tccaggagat tctcgacgga ctgccggacg gcgtgcgggt
      361 gatccgcgca tccgacaagc gcgtggtgct ggccaaccgc gccttctgcc agcaggtcgg
      421 tcgcacgcag tcggaaatcc tcggcgctgc ctgccatcgc agcagccacg ggcgggcgga
      481 gccctgcgca ccgacgctgg tcgtgtgccc gcttgccgaa ttgcgcgagg tgggggcgac
      541 attgaaggcc acgcatcacc acctgcgcgc cgacggcacg cagtttcccg ccgaagtcag
      601 cgccgtgctg gtcgaaatcg caggggatgc cggacccgag cgctatgtcg tcgaatccat
      661 ccgcgacctg ggccaagccg tgcgcatctc gcacgagcag cggctgtcgg aactcggcct
      721 gctcgcggcc ggcatcgccc acgagatcca caacccgctg ggatcggtgc ggctcggcgt
      781 gcagggactg acgcgcgaga tccacgaggg gcgcatccgc cccgagcaga tcgccgagta
      841 catggggctc atcgaccagg agatcgacaa ctgcatcgcc gtgacgcgac gcctgctgct
      901 gctggcacgc ccgccggacc gaagcgtgca actggtcgtg ctcggcgatg cgctgcgcga
      961 caccctgcgc ctgctcgagt tcgacgccca ggcgcgcggc atacggcagc gggtagaggt
     1021 gcccgcggag gagctgcgcg tcttcgccga cgaggccgag atacgcatga tcttcctcaa
     1081 cctgctgcag aacgcgcacc acgcgatgcc cgacgggggc gatctgacgg cgcggctggc
     1141 gcgcgacggc gaccatgcgg tcatcgagat cgccgatacc ggcgtcggca tggagcccga
     1201 acacgcatcg cgcatcttcg atccgttcta cagccgtcgc gcggacggcg tttccggcac
     1261 cggcctcggc ctcaccatcg tgcgcaactt catccagcgc accggcggcg aagtcggcgt
     1321 cgacagcgcg cccggcaagg gcacgcgatt caccatacgg ttgctgctcg cagagcattc
     1381 cccgggggtt ccgagttgaa aagaaagacg ctcctcatca tcgacgacga cgccatcttc
     1441 aacggcctgc tgcgccggca gatcgaatcg atgggattcg ccgccgtcgg cgccggctcg
     1501 tgggccgagg cacagcagaa gctcggcgag atcgagccgg atgccatcat cctcgacttc
     1561 aagctgcccg acagcgacgc cacccgcgtc ctcggcgaga tccgcaacca gtatccggtg
     1621 atcgtgctca ccggcttcgg ctccatcccg aacgccgtca gcgcgattcg cgagggcgcg
     1681 gccgacttcc tgaccaagcc cgtgaatgtc gacgaactcg agatgacggt gcgccgcgtc
     1741 ctcgagaccg ccgaactgcg ggaatcgaac cagttctacc ggatgcagct ggcggcccat
     1801 cgccccggcc cgctggtgtt cgacagcgca gcgatgcgcg aggtgcaggc gatgatcgac
     1861 gccgtcggcc ccaccgacac gacggtgctg atcctcggcg aatcgggcac gggcaaggaa
     1921 ctcgtcgccc aggccatcca cgaccgcagc gcgcgcgccc agcgcgagct ggtcgccatc
     1981 gacggcagcg gactgcagga aagcctgttc gagtccgagc tgttcggtca cgagcggggc
     2041 gccttcacgg gggccgaccg gcagaagaag ggcctcatcg aggaagcgac cggcagcacg
     2101 ctgttcctcg atgaagtcgg cgacgtcggc gcggcgatcc aggccaagct gttgcgcgtc
     2161 atggaaacgg ggacattccg gcgcctgggc ggcaacaaga cgctgacggc cgacgcgcgc
     2221 ttcgtcgcgg cgaccaaccg cgacatcgag gcgatgagcc gcgacggccg cttccgcagc
     2281 gacctgtatt tccgcctttc gcgcttcgtc atcaccatcc cgccgctgcg cgagcgtcgg
     2341 gaggacgtcg aaccgctcgc ccgtcatttc ctcgcgctgc ttacgcgtac cgcgccgatg
     2401 tccatcgccc cggaggccat gaaggtgctg cttggctacg actggccggg caatgtgcgc
     2461 gagctgcgca acgccgtgga gcgcgcgatc atcctggcgc ggcccggcag ccaggtgcgc
     2521 gccgagcacc tttcgttcat cccgcgcgcc ctggccggcg agaccgtgct gcgcttcgcc
     2581 ggcgagccga cgctggagca gatcgagcgc gagtacctgc gtcaggtgct gagccgcctg
     2641 ggcggcaaca ggcagaagac ggctgccgtg ctcggcatca gcgagcgcaa cgtgtatcgg
     2701 ctcatcgaga agcaccgcac ggcctgagtc gggcgggatt gtcccggggg ggccttcttc
     2761 gctataattc gcgccctgca cgccaggtgc aggtcggatg gtggctgtag ctcagttggt
     2821 agagtcccgg attgtgattc cggttgtcgt gggttcgagt cccatcagcc accccac
//
LOCUS       DAOQJH010000371         2877 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_23754_length_2877_cov_3.798724, whole genome shotgun sequence.
ACCESSION   DAOQJH010000371 DAOQJH010000000
VERSION     DAOQJH010000371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2877)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2877)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2877
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23754_length_2877_cov_3.798724"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2877)
                     /locus_tag="PLQ18_08890"
     CDS             complement(<1..>2877)
                     /locus_tag="PLQ18_08890"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNE01808.1"
                     /translation="QAITRVKPRLAFVGGVDNLSVQAVDDGRPVARPLSVTVRGDDPA
                     EVRRAADRVVDILRSLIGVTDVVDDEDQAEAAELRLVLKPGALLRAGLSAPQLLRELR
                     LLGEGETIAQLREQGRNVDVLLRGKAAPEPFFDIEEFLARPVRLPDGRSAPLGEFASA
                     EPASGRSVVRHQNLRRAISIEADVDQARVNTFALNRVIVAEWERTAAAGLPGVELVIG
                     GAFGSVAGHLGALVSLFALGSGLGYLVLATRAHSYRQPLVIFAAVPFAALAFVVGLAV
                     SGQPVGPYTGFAGTALLGLSVQSTLLLVSAVQRLLPIEGSVARAVALAAADRALPVLV
                     SALASVAGLAVFALGVPVAAPAWMALAGALIGGIPVIALCVLLVLPAICLYVLRAPVS
                     AVTAINVVDAEPGGGGWRERMRRGRRHAAIVALNESELNNAEPELRARHEAGLDALKV
                     GEWLQALRLFEDAAKRHPEAVQLQFGATQALLQYLFENEWDEGYHARAGRHLARARQL
                     ASGDPRLRPLERALAELEARHVPVAGEGTSSQPRVGMLRLSGTVGRPWRPASVARGQR
                     APDRGTDLPAGLHDLALALRNLVRQRKRTAAALGAIGFGAVALILAGGFIEWVLWATR
                     ESAVYGQLGHVQVVRQDFFVRGTADPRGFLIPGDAPPAGLRELPHVRAVTPRLSFSGL
                     ASTGETTVPYLGQGVVPEDEAPISIDYRIVSGKGLTTSDAREVIVGTGLAATLGVKPG
                     QKLILMSNSGSGAFTPVELKVRGLFSTSNKALNDVALRVPLKTAQDLLQVDGVHIWVV
                     LLDRTESTAAVLPQLRALLPAGLQAVPWSDLADFYNKTSELFQRQMFVLRLMIAVIIL
                     LSISNSLMMNVMERTGEIGTLCALGTRRRSILKIFSLEALALGLLGAATGTALGVTIA
                     SVASAVGIPMPPAPGMDKGFVAEILVTPALAGSTFGLAVIATFVS"
BASE COUNT          436 a         1071 c         1024 g          346 t
ORIGIN      
        1 ggagacgaag gtcgcgatga cggccagccc gaaggtcgag cccgccagcg ccggcgtgac
       61 caggatctcg gcgacgaagc ccttgtccat gcccggggcc ggcggcatcg ggatgccgac
      121 cgccgaggcc accgaggcga tggtcacgcc gagcgccgta ccggtcgccg cgccgagcag
      181 gccgagcgcg agggcttcga ggctgaagat cttcaggatc gagcgccggc gcgtgccgag
      241 cgcgcacagc gtgccgatct cgccggtgcg ctccatgacg ttcatcatca gcgagttcga
      301 gatgctgagc aggatgatca ccgcgatcat cagccgcagc acgaacatct ggcgctggaa
      361 cagctcgctg gtcttgttgt agaagtcggc gaggtccgac cacggcaccg cctgcaggcc
      421 agccggcagc agggcgcgca gctgcggcag cacggcggcc gtcgattcgg tgcgatcgag
      481 cagcaccacc cagatgtgca cgccatcgac ctgcagcagg tcctgtgcgg tcttcagcgg
      541 cacgcgcagg gcgacatcgt tcagcgcctt gttcgaggtc gagaacagcc cgcgcacctt
      601 cagctcgacc ggcgtgaacg cgccgctgcc ggagttgctc atcaggatca gcttctggcc
      661 gggcttgacg ccgagcgtcg ccgcgagccc ggtgccgacg atgacctcgc gcgcgtcgct
      721 ggtggtcagg cccttgccgc tgacgatgcg gtaatcgatg ctgatcggcg cctcgtcctc
      781 gggcaccacg ccctgaccga ggtacggcac cgtggtctcg ccggtgctcg ccagaccgct
      841 gaagctcagc cgcggcgtca cggcgcgcac gtgcggcagc tcgcgcaggc cggccggcgg
      901 cgcatcgccc gggatcagga agccgcgcgg atcggccgtg ccgcgcacga agaaatcctg
      961 gcgcacgacc tgcacatggc cgagctggcc gtagacggcc gattcgcgcg tcgcccacag
     1021 cacccattcg atgaagccgc cggcgaggat cagcgccacg gcgccgaagc cgatcgcgcc
     1081 gagcgcggcg gccgtgcgct tgcgctggcg cacgaggttg cgcagcgcca gcgccagatc
     1141 atgcaggccg gccggcagat cggtgccgcg atcgggcgcc cgctgcccgc gcgccacgct
     1201 cgccggccgc cacggccggc cgaccgtgcc cgacagccgc agcatgccga ccctgggctg
     1261 gctgcttgtg ccctccccgg ccaccggcac atgccgggct tcgagttcgg ccagcgcccg
     1321 ttccagcgga cgcaggcgcg gatcgccgga agcgagctgg cgcgcgcgcg ccagatgccg
     1381 gccggcacgg gcgtgatagc cctcgtccca ttcgttctcg aacagatact gcagcagcgc
     1441 ctgcgtggcg ccgaactgca gctgcacggc ctcggggtga cgcttggcgg cgtcctcgaa
     1501 caggcgcagc gcctgcagcc actcgccgac cttcagcgcg tcgagaccgg cctcgtggcg
     1561 ggcgcgcagc tcgggctcgg cattgttcag ctcggattcg ttcagcgcga cgatcgccgc
     1621 atgccgccgg ccgcggcgca tgcgctcgcg ccagccgccg ccgcccggtt cggcgtcgac
     1681 gacgttgatc gccgtgaccg ccgacaccgg cgcgcgcagc acgtacaggc agatcgccgg
     1741 cagcaccagc agcacgcaca gcgcgatgac cggaatgccg ccgatcagcg ccccggcgag
     1801 cgccatccac gccggcgccg ccaccggcac gccgagcgcg aataccgcga ggccggcgac
     1861 gcttgccagg gccgagacca gcaccggcag cgcccggtcg gccgcggcga gcgccacggc
     1921 tctggcgacc gagccctcga tcggcagcag ccgctgcacg gccgacacca gcagcagcgt
     1981 cgactgcacc gacagaccga gcagcgccgt gccggcaaag ccggtgtacg gaccgaccgg
     2041 ctggccgctg acggccagcc cgacgacgaa ggccagcgcc gcgaacggca cggccgcgaa
     2101 gatcaccagc ggctggcggt agctgtgcgc gcgcgtcgcg agcaccagat aaccgagtcc
     2161 gctgccgagc gcgaacaggc tgaccagcgc accgaggtga ccggcgacgc tgccgaaggc
     2221 gccgccgatg accagctcga cgccgggcag gccggcggcg gccgtgcgct cccattcggc
     2281 gacgatcacg cggttcagcg cgaaggtgtt gacgcgcgcc tgatcaacgt cggcctcgat
     2341 gctgatcgca cggcgcaggt tctggtggcg cacgacgctg cggccgctgg ccggctcggc
     2401 gctggcgaac tcgccgagcg gggcgctgcg gccatcgggc aggcgcaccg gccgcgccag
     2461 gaattcctcg atgtcgaaga acggttcggg cgcagccttg ccgcgcagca gcacgtcgac
     2521 gttgcggccc tgctcgcgca gctgcgcgat ggtctcgccc tcgccgagca ggcgcagctc
     2581 gcgcagcagc tgcggcgccg acagcccggc gcgcagcagc gcccccggtt tcagcaccag
     2641 gcgcagctcc gcggcctcgg cctgatcctc gtcgtcgacc acatcggtga cgccgatcag
     2701 gctgcgcagg atgtcgacga cgcggtcggc ggcgcgacgc acctcggccg gatcatcgcc
     2761 gcgcacggtg acgctgagcg gccgcgccac cggccggccg tcgtcgacgg cctgcacgct
     2821 caggttgtcg acgccaccga cgaaggcgag ccgcggcttg acgcgcgtga tcgcctg
//
LOCUS       DAOQJH010000372         2864 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_23940_length_2864_cov_4.613742, whole genome shotgun sequence.
ACCESSION   DAOQJH010000372 DAOQJH010000000
VERSION     DAOQJH010000372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2864)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2864)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2864
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23940_length_2864_cov_4.613742"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..600)
                     /locus_tag="PLQ18_08895"
     CDS             complement(<1..600)
                     /locus_tag="PLQ18_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011628432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S cluster-binding domain-containing
                     protein"
                     /protein_id="HNE01809.1"
                     /translation="MIAAVAHADDTPATDGSGARLLTDPATLLERLGLDAALLPAARR
                     AAERFPMRVPAGFVARMHPGDPADPLLRQVLPLADELATAGGFGADPVGDAAARVAPG
                     LLHKYAGRALLLVTGACAIHCRYCFRREYPYADTGASPAGWEAAFAHLHADRSIRELI
                     LSGGDPLTLSNRRLRELVQAADAVPQLERLADRLFATIPA"
     gene            651..1220
                     /gene="efp"
                     /locus_tag="PLQ18_08900"
     CDS             651..1220
                     /gene="efp"
                     /locus_tag="PLQ18_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013820328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HNE01810.1"
                     /translation="MATYSTNEFKSGLKIMLDGDPYAIVENEFVKPGKGQAFNRVRVR
                     NLKSGRVIERTFKSGDSVEGADVLDIDMQYLYCDGEFWHFMNPTSFEQLAADATAVGD
                     NARWLKENDLCQLTLWNGVPLSVLPPNFIIARITETDPGLKGDTSGGGGKPATLETGA
                     VVRVPLFVQTDEVIKVDTRTGEYVSRVKE"
     gene            1269..2264
                     /gene="epmA"
                     /locus_tag="PLQ18_08905"
     CDS             1269..2264
                     /gene="epmA"
                     /locus_tag="PLQ18_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012970956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P lysine aminoacylase EpmA"
                     /protein_id="HNE01811.1"
                     /translation="MTPLSGPADWRPSCAPEALRLRAGLLARMRAFFEARGVLEVDTP
                     ALSRAAVTDPALASFAVDWHGPEPGRLWLQTSPEYAMKRLLAAGSGPIWQLAKVFRDG
                     EAGRRHNPEFSLLEWYRPGFDHHALMDEVIDLVSELLAGRRALAEPERIRYQDAFLQH
                     AGVDPFAADVPALRVACTRHALVPPPGMPEAQVEPWLDLLLSHVIEPRLGCGRLSFLY
                     DYPPAQAALACIRRDETPAVGSRFELYLDGVELANGFHELGDAGEQRARFEADNRARV
                     RAALPVMPIDEALLAALASGLPACAGVALGFDRLLMLAANAGGLDAVLAFPVDRA"
     gene            complement(2382..2771)
                     /locus_tag="PLQ18_08910"
     CDS             complement(2382..2771)
                     /locus_tag="PLQ18_08910"
                     /inference="COORDINATES: protein motif:HMM:TIGR02809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HNE01812.1"
                     /translation="MYDLDPFNEALARSATWIAPVVQANKLFVASLEKLAAFQFGLLR
                     SYAGFGLGRLQAASDVTDVESLKAFSAAQLEAAGALRQKALDDAQAYADLLAGFKAEF
                     EQLIAANSTDFAPKPIVKVARRAASAA"
BASE COUNT          436 a          948 c         1051 g          429 t
ORIGIN      
        1 ggcgggaatg gtggcaaaca gccggtcggc taagcgttcg agttgcggca cggcgtcggc
       61 ggcctgcacc agctcacgca gccggcggtt cgacagcgtc agcggatcgc cgcccgacag
      121 gatcagctcg cggatcgacc ggtcggcgtg caggtgcgcg aaggccgcct cccagccggc
      181 gggactggcg ccggtgtcgg cataggggta ctcgcgccgg aagcagtagc ggcagtggat
      241 ggcgcaggca ccggtcacga gcagcagcgc ccgtccggca tacttgtgca gcaggcccgg
      301 cgcgacgcgt gctgcggcat cgccgaccgg atcggcgccg aagcccccgg ccgtggcgag
      361 ctcatcggcg agcggcagga cctggcgcag cagcggatcg gccggatcgc cgggatgcat
      421 gcgcgcgacg aatccggccg gtacccgcat cggaaaccgt tctgccgcac ggcgcgccgc
      481 cggcagcagg gccgcatcga ggccgagccg ttcgagcagg gtggcgggat cggtgagcag
      541 acgggccccg gacccgtcgg tggcaggcgt gtcgtcggca tgcgcgacag cggctatcat
      601 cgtcggcctt ctcaactcgg atacgctccg ttccacgcag gtcagcgacg atggcgactt
      661 atagtaccaa cgaattcaag tcgggtctga agatcatgct cgatggcgac ccgtatgcca
      721 tcgtcgagaa cgaattcgtc aagcccggca agggccaggc cttcaaccgc gtgcgcgtgc
      781 gcaacctgaa atccgggcgc gtgatcgagc gcacgttcaa gtccggcgac tcggtcgagg
      841 gcgccgacgt gctcgacatc gacatgcagt acctgtactg cgacggcgag ttctggcact
      901 tcatgaaccc gaccagcttc gagcagctcg ctgccgatgc cacggccgtc ggtgacaacg
      961 cccggtggct gaaggaaaac gacctctgcc agctgacgct gtggaacggc gtgccgctgt
     1021 cggtgctgcc gccgaacttc atcatcgccc gcatcaccga gaccgacccg ggcctgaagg
     1081 gtgacacctc cggcggcggc ggcaagccgg cgacgctcga aaccggcgcg gtcgtgcgcg
     1141 tgccgctgtt cgtgcagacc gacgaggtga tcaaggtcga cacgcgcacc ggcgaatacg
     1201 tttcgcgcgt caaggaatga accgtccttg ccggtgccgt gccgccccgg aatttccgtc
     1261 cgggcatcgt gacgccgttg tccggcccgg ctgactggcg gccgtcgtgt gcgcccgaag
     1321 ccctgcgcct gcgcgccggg ctgctcgcgc ggatgcgcgc ctttttcgag gcgcgcggcg
     1381 tgctcgaagt cgacaccccg gcgctgtcgc gggcggcggt gaccgatccg gcgctggcga
     1441 gcttcgccgt cgactggcac ggcccggagc ccgggcggct gtggctgcag acttcgccgg
     1501 agtacgcgat gaagcggctg ctcgccgccg gcagcggccc gatctggcag ctcgccaagg
     1561 tgtttcgcga cggcgaggcc ggtcgccggc acaaccccga attctcgctg ctcgaatggt
     1621 accggcccgg ctttgatcat cacgcgctga tggacgaggt catcgacctc gtcagcgagc
     1681 tgctcgccgg caggcgtgcg ctcgccgagc ccgaacgcat ccgctatcag gacgctttcc
     1741 tgcagcacgc cggcgttgat ccgttcgctg cggacgtgcc ggcgctgcgg gtggcctgca
     1801 cccggcacgc gctggtaccg ccgcccggca tgccggaagc gcaggtcgaa ccgtggctgg
     1861 acctgctgct cagtcacgtg atcgagccgc ggctcggctg cgggcggctg agcttcctgt
     1921 acgactatcc gccggcgcag gccgcgctcg cgtgcatccg ccgtgacgag acgccggccg
     1981 tcggcagccg tttcgagctg tatctggacg gcgtcgaact ggccaacggc ttccacgaac
     2041 tcggcgatgc cggcgagcag cgcgcccgtt tcgaggccga caatcgcgcc cgtgtgcggg
     2101 ccgccctgcc ggtgatgccg atcgacgaag cgctgctggc ggcgctggcc agcggtctgc
     2161 cggcctgcgc cggcgtcgcg ctcggcttcg accggctgct gatgctcgcg gccaatgccg
     2221 gcgggctgga tgcggtgctg gcctttccgg tcgatcgcgc ctgagcgggg ccggaaccgc
     2281 ggcgtgcgct ccggccgggg gaaccccgga ggacatgaaa aaagcccggc tgcacgagcc
     2341 gggccggatg ctgctgcgcc gggggagggc gggacgtgcg atcaggcggc cgaagcggcg
     2401 cggcgagcga ccttgacgat cggcttcggc gcgaagtcgg tgctgttggc ggcgatcagc
     2461 tgttcgaact cggccttgaa gccggcgagc aggtcggcgt aggcctgggc gtcgtcgagc
     2521 gccttctggc gcagcgcgcc ggcggcttcg agctgggcgg ccgagaaggc cttcaggctc
     2581 tcgacatcgg tcacgtcgct ggcggcctgc aggcggccga ggccgaagcc ggcataggag
     2641 cgcagcagcc cgaactggaa ggcggcgagc ttttcgaggc tggcgacgaa cagcttgttg
     2701 gcctgcacca ccggcgcgat ccaggtggcg gaacgggcaa gggcttcgtt gaacgggtcg
     2761 aggtcgtaca tggcgggcat cccggcggaa gtgaaggaat aaggtgaagg aagtttctgt
     2821 tgcgatgcag cataacagcg tggatgttgc gtcgcaacaa tcga
//
LOCUS       DAOQJH010000373         2862 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_23964_length_2862_cov_3.267189, whole genome shotgun sequence.
ACCESSION   DAOQJH010000373 DAOQJH010000000
VERSION     DAOQJH010000373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2862)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2862)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2862
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23964_length_2862_cov_3.267189"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1622
                     /locus_tag="PLQ18_08915"
     CDS             <1..1622
                     /locus_tag="PLQ18_08915"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE01813.1"
                     /translation="LGLSISRRLVAAAGGVLGAQRRTGGGSEFRFTWPVAPVPVPPGA
                     DAAQPPLPPGLVLLVAQDTFADLGDPLPVLDRLRVPVTALALAQLSAAARRAGPALMP
                     VLAWPHLDTDGRRAAVIDWLAAHPELAARTLLVVARGEPSEPAPSLRRQVARVLAHPP
                     LALLFDQTTLSAVSPQLPLAGYRVAVVEDNPVNQLVVTQYLERAGAEVSVHDQGGELL
                     DWLAQRVSGEGPQLILMDVQMQGMDGIEATRRLRADARWCGLPVVALTAHAFADEIER
                     CLAAGMNAHLSKPIEPSKLTEAVLHHALPPPGRPVVAAAAPDGPPVSPVVPDAVLLGL
                     RSIDGARLHERLGDGARAALCELLARAGDGENELLRLIVDNRRAELQRRAHQLKGVGG
                     ALCAHALAEAAAALEKRLRADAEGHEPLPVVETIRLADEYARVLGELREAGYTPAELP
                     PAAAGIDFVHGLARLKALCAATDLEALALARRLGEAQSVGWSAARRRDWAAAVAALER
                     YDFSAAEAGFARFVPSTHDTDRQEEPPHDSS"
     gene            1609..>2862
                     /locus_tag="PLQ18_08920"
     CDS             1609..>2862
                     /locus_tag="PLQ18_08920"
                     /inference="COORDINATES: protein motif:HMM:NF013502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HNE01814.1"
                     /translation="MTRRNRPRAGVPEPGSGVASRASPEADIPAPAEAPADGPPLPGA
                     AAPFLQEILPGSPAEAATETATEAATPAAGREPPGDLYVVGIGASAGGLEALRPLIAN
                     LVPNGRLSYLIAQHMSTQHRSLLVDLLARDAQVLVVEATGGAWLRPDTVYVTPPDRDI
                     LVSSGRIVLRRPGADIGPRPSVDALLNSLATEYAERAIGVILSGTGSDGAHGCRAIKA
                     RGGFTIVQEPGSAKYDAMPRAAQRADAVDLALPPELIARRLPELVDAPAGAVALQSAE
                     EGSGAEGGENGALRGLLERVLRATQTDFTDYKEATLGRQIGRRLAALRLESLSAYLDY
                     VDQRPEELGVLQRSFLISVTEFFRDPDAFRALERALEPLIAGHGGQGPIRVWVPGCAT
                     GEEAYSLAMLFAQRLGSRAVGSVKIF"
BASE COUNT          342 a         1076 c         1060 g          384 t
ORIGIN      
        1 gcctcgggct gtcgatcagc cggcgactgg tcgcggcggc cggcggcgtg ctcggcgcgc
       61 agcggcgcac cggtggcggc agcgagttcc gcttcacatg gccggtcgcg ccggtgccgg
      121 tcccgcccgg ggccgatgcc gcgcagccgc cgctgccgcc ggggctggtg ctgctggtcg
      181 cgcaggacac cttcgccgat ctcggcgatc cgctgccggt gctggaccgc ctgcgcgtgc
      241 cggtcaccgc cctggcgctc gcgcagcttt cggccgccgc ccggcgcgcc gggccggcgc
      301 tgatgccggt gctggcctgg ccgcacctcg ataccgatgg ccggcgtgcg gccgtcatcg
      361 actggctggc cgcgcacccg gagctggcgg cgcgcacgct gctggtggtc gcccgcggcg
      421 agccctccga accggcgccg tcgctgcgcc ggcaggtcgc gcgcgtgctc gcgcatccgc
      481 cgctggcgct gctgttcgac cagaccacgc tgagcgccgt ctcgccgcag ctgccgctcg
      541 ccggctaccg ggtggcggtg gtcgaggaca acccggtcaa ccagctcgtg gtcacgcagt
      601 atctggagcg ggccggggcc gaggtctcgg tccacgacca gggtggcgag ctgctcgact
      661 ggctcgcgca gcgtgtgagc ggggagggtc cgcaactgat cctgatggac gtgcagatgc
      721 agggcatgga cggcatcgag gcgacgcggc ggctgcgcgc cgatgcgcgc tggtgcgggc
      781 tgccggtcgt ggcgctgacc gcgcatgcct ttgccgacga gatcgagcgc tgcctcgcgg
      841 ccggcatgaa cgcgcatctg tccaagccga tcgagccttc gaagctgacc gaggcggtgc
      901 tgcaccacgc gctgccgccc cccggccggc cggtcgtagc ggcggcggcc ccggacggcc
      961 cgcccgtgtc gcccgtggtg ccggacgcag tgctgctcgg gctgcgcagc atcgacggcg
     1021 cgcggctgca cgagcgcctc ggcgacggcg cgcgggccgc gctctgcgag ctgctggccc
     1081 gcgccggcga cggcgagaac gagctgctgc gcctgatcgt cgacaaccgg cgcgccgagc
     1141 tgcagcgccg tgcgcatcag ctcaagggcg tcggcggcgc gctgtgcgca cacgcgctcg
     1201 ccgaggccgc cgccgccctc gaaaagcggc tgcgcgccga tgccgagggc cacgaaccgc
     1261 tgccggtcgt cgagaccatc cggctcgccg atgaatatgc gcgggtgctg ggagaactgc
     1321 gcgaggccgg ctacaccccc gccgagctgc cgccggcggc ggccggcatc gatttcgtcc
     1381 acggactcgc acgcctgaag gcgctctgcg cggccaccga tctcgaagcg ctggcgctgg
     1441 cgcgccggct cggcgaggcg cagtctgtcg gatggtcggc cgcccggcgc cgggactggg
     1501 cggcggccgt tgccgcgctc gagcgctacg atttctccgc tgcggaagcc ggtttcgccc
     1561 gcttcgttcc ttcaacgcac gacacggacc gtcaggaaga gcctccccat gactcgtcgt
     1621 aaccgcccac gcgccggcgt gccggagcca ggctccggcg tcgcctcgcg cgcctccccc
     1681 gaagcggaca ttccggcacc ggccgaagcg ccggcggacg ggccgccgct gcccggggcc
     1741 gctgctccgt tcctgcagga gattcttccg ggcagtccgg ccgaggccgc gaccgagacc
     1801 gcaaccgagg ccgcgacccc cgcggccggg cgcgagccgc cgggcgatct gtacgtggtc
     1861 ggcatcgggg cctcggccgg cggtctcgaa gccctgcgcc cgctgatcgc caacctcgtg
     1921 ccgaacggcc ggctgagcta cctgatcgcg cagcacatgt cgacgcagca ccgcagcctg
     1981 ctggtcgacc tgctggcgcg cgatgcgcag gtgctcgtgg tcgaggcgac cggcggtgcc
     2041 tggctgcgtc cggacacggt ctacgtgaca ccgccggatc gcgacatcct ggtttcgagc
     2101 ggccgcatcg tgctgcgccg ccccggcgcc gacatcggcc cgcggccctc ggtcgatgcg
     2161 ctgctgaact cgctcgcgac cgaatacgcc gagcgcgcga tcggcgtgat cctctccggc
     2221 accggctccg acggcgcgca cggctgccgc gcgatcaagg cgcgcggcgg cttcacgatc
     2281 gtgcaggagc cgggcagcgc caagtacgac gcgatgccgc gcgctgccca gcgcgccgat
     2341 gccgtcgatc tggcgctgcc gcccgagctg atcgcccggc gcctgcccga gctggtcgac
     2401 gcgccggccg gagcggtggc gctgcaatcg gccgaggaag gcagcggggc cgagggcggg
     2461 gaaaacggcg cgctgcgcgg cctgctcgaa cgcgtgctgc gcgccacgca gaccgacttc
     2521 accgattaca aggaagcgac gctcgggcgc cagatcggcc ggcggctcgc ggcgctgcgc
     2581 ctcgaatccc tgagcgccta cctcgattac gtcgaccagc gccccgagga actcggcgtg
     2641 ctgcagcgca gcttcctgat ctcggtgacc gagttcttcc gcgatccgga tgccttccgc
     2701 gcgctcgaac gcgcgctcga accgctgatt gccgggcacg gcggccaggg gccgatccgc
     2761 gtctgggtgc cgggctgcgc gaccggcgag gaagcctatt cgctggcaat gctgttcgcg
     2821 cagcgcctcg gcagccgcgc cgtcggcagc gtcaagatct tc
//
LOCUS       DAOQJH010000374         2858 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_24018_length_2858_cov_2.742419, whole genome shotgun sequence.
ACCESSION   DAOQJH010000374 DAOQJH010000000
VERSION     DAOQJH010000374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2858)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2858)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2858
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24018_length_2858_cov_2.742419"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..599)
                     /locus_tag="PLQ18_08925"
     CDS             complement(<1..599)
                     /locus_tag="PLQ18_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020202500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HNE01815.1"
                     /translation="MSPPRTVRAAAVQISPVFESADGTLGKVCDAIDEAALRGVELIV
                     FPETFVPYYPYFSFVRPPVQAGREHLRLHALSPTVPGPLTERVSERARRHGMVVVLGI
                     NERDHGTLYNAQLIFDADGTLLLKRRKITPTYHERMIWGQGDGAGLRPVDTRVGRVGA
                     LACWEHYNPLARYALMAQHEEIHCAQFPGSLVGPVFAEQ"
     gene            complement(610..1092)
                     /locus_tag="PLQ18_08930"
     CDS             complement(610..1092)
                     /locus_tag="PLQ18_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008920862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSMEG_0572 family nitrogen starvation response
                     protein"
                     /protein_id="HNE01816.1"
                     /translation="MPAVTRPAHATGEYLVDYEEKVFEDVKAAPGEKALVTFHTVAFE
                     GSVGLVNLLQATRLQRKGYETTILLYGPGVTLGVQRGFPTLGDEAFAGHQNYAKQLTR
                     FIQEGGKVYACRFALQALYGHGEGALVEGIRPIHPLDVLDLMLLHRKAGAVILDTWTL
                     "
     gene            1505..>2858
                     /locus_tag="PLQ18_08935"
     CDS             1505..>2858
                     /locus_tag="PLQ18_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019919589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HNE01817.1"
                     /translation="MSHTTRFWPQRLRSADGPAYLALADLIADDLRSGRLKSGDRLPP
                     LRELAAALGLNYSTVARGLAEARKRGLIDSRVGSGTYIRGAAPALPLRDGNSADMTMN
                     LPPEPDDPALLAQLQDSTAAAMRAADPWALLRYQDFGGSPQQREAGAHWLHTRFPEAR
                     AGQLMVCPGVHAVLYALLSQLARPGDTICVEALSYPGIKAIAAQLGLRLLALADDGEP
                     CAAALRDACRTAAPKALYLNPTLHNPSTRTLSRSRREALAEVALAYSLPIIEDDAYGR
                     LGRTAPAAFASLAPSLTYYVTGLSKTVGAGLRIAYVHAPGQRPMQRLAGALRALTVMA
                     SPLTTAIATRWIDDGTAARLVEAIRDESAQRLQLAARLLAAHPLRAHPEGFHVWLPLP
                     AGFDANVLAAWLRERGVPAVAAAAFSTDAQPPDALRVNLGGPGTRAQCAAGLQLVAEA
                     L"
BASE COUNT          430 a         1009 c          995 g          424 t
ORIGIN      
        1 atctgctcgg cgaacaccgg cccgaccagc gagccgggga actgcgcgca gtggatttcc
       61 tcgtgctgcg ccatcagcgc gtagcgcgcc agcgggttgt agtgctccca gcaggccagc
      121 gcgccgaccc gcccgacgcg ggtgtcgacc ggccgcagac cggcgccgtc gccctgaccc
      181 cagatcatgc gttcgtggta ggtcggcgtg atcttgcggc gcttcagcag cagcgtgccg
      241 tcggcgtcga agatgagctg ggcgttgtag agcgtgccgt gatcgcgctc gttgatgccg
      301 agcacgacga ccatgccgtg gcgccgggcg cgctccgaca cgcgctcggt cagcggcccc
      361 ggcacggtcg gcgacagcgc gtgcaggcgc aggtgctcgc gcccggcctg caccggcggg
      421 cgcacgaacg agaagtacgg gtagtagggg acgaaggtct ccgggaacac gatcagctcg
      481 acgccgcgca gggcagcctc gtcgatcgca tcgcagacct tgccgagcgt gccgtcggcg
      541 gactcgaaca cgggcgagat ctggacggcg gcggcacgta ccgtgcgcgg cgggctcatg
      601 gcgacggcct cagagcgtcc aggtgtcgag gatcaccgca ccggccttgc ggtgcagcag
      661 catcaggtcg agcacatcga gcggatggat cggccggatg ccctcgacca gcgcgccctc
      721 gccgtggccg tacagcgcct gcagcgcgaa gcggcaggcg tagaccttgc cgccttcctg
      781 aatgaagcgc gtgagctgct tcgcgtagtt ctggtgcccg gcgaaggcct cgtcgccgag
      841 cgtcggaaag ccgcgctgca cgccgagcgt gacgccgggg ccgtacagca ggatcgtggt
      901 ctcgtagccc ttgcgctgca ggcgcgtcgc ctgcagcagg ttgaccagac cgaccgagcc
      961 ttcgaaggca acggtatgga aggtgaccag cgccttttcg ccgggcgcgg ctttcacatc
     1021 ctcgaaaacc ttttcttcgt aatcgaccag atattcgccg gtggcatggg cggggcgggt
     1081 gactgcgggc atcgggattc tccggtgaat tcgagccggg atcggcagga ttcgactcgc
     1141 agcaaccgtg ccatcgcttt ggcaggtgat gcgatcaatc tgcgatgaag ccgccggcca
     1201 ctgcctgcaa atgcaggtgc gaatccggaa ccgggcgatg atcgatcgcc gcgcagcctg
     1261 ccgatcaatt atcaatcacc gggatttacg cgccatgcgc aggcattgcg cctccggaca
     1321 gtgcgaagat taaaaaaaca taaccatgaa caggctccgg tatctggccc gatgcattca
     1381 atctgcgatc aatccattcc ggtttccggg ggcgtgacca taagaatccg gattacactc
     1441 ggcggtgtgt acggccctgt gcgatttcct gcggaaaccg cctgcattcc ggagtccggc
     1501 tgccatgtcc cacaccacgc gtttctggcc gcagcgcctg cgttcggccg acggaccggc
     1561 gtatctcgcg ctcgccgacc tgattgccga cgatctgcgc agcggccggc tgaaatccgg
     1621 cgaccggctg ccgccgctgc gggagctggc cgcggccctc gggctcaact attcgacggt
     1681 ggcgcgcggc ctcgccgagg cgcgcaagcg cggcctgatc gacagccgcg tcggcagcgg
     1741 cacctacatc cgcggtgcgg caccggcgct gccgctgcgc gacggcaaca gcgccgacat
     1801 gacgatgaac ctgccgccgg agcccgatga ccccgcgctg ctcgcgcagc tgcaggacag
     1861 tacggctgcg gcgatgcgcg ccgccgatcc ctgggcgctg ctgcgctatc aggatttcgg
     1921 cggcagcccg cagcagcgcg aggccggcgc gcactggctg cacacgcgct ttccggaggc
     1981 gcgcgccggg cagctgatgg tctgccccgg cgtgcatgcg gtgctctacg cgctgctgag
     2041 ccagctcgcg cggcccggcg acaccatctg cgtcgaggcg ctgagctatc ccggcatcaa
     2101 ggccatcgcc gcccagctcg gcctgcgcct gctcgcgctc gccgatgacg gcgaaccctg
     2161 cgcggcggcg cttcgcgatg cctgccggac cgcggcgccg aaggcgctgt acctgaaccc
     2221 gacgctgcac aacccgagca cgcgcacgct gtcgcgcagc cggcgcgagg cgctggccga
     2281 ggtcgcgctg gcctacagcc tgccgatcat cgaggacgac gcctacggcc ggctggggcg
     2341 cacggccccg gccgccttcg cgagcctcgc accatcgctg acctactacg tgaccgggct
     2401 ttcgaagacg gtcggtgccg gcctgcgcat cgcctacgtg catgcgccgg gacagcgccc
     2461 gatgcagcgg ctcgccgggg cgctgcgtgc gctgacggtc atggcctcgc cgctgacgac
     2521 ggcgatcgcc acacgctgga tcgacgacgg cacggccgcg agactggtcg aggccatccg
     2581 cgacgagtcc gcgcagcgcc tgcagctcgc cgcgcgcctg ctggccgcgc atccgctgcg
     2641 cgcgcatccc gaaggctttc atgtctggct gccgctgccc gcgggcttcg acgcgaacgt
     2701 gctggccgcc tggctgcgcg agcgcggcgt gccggccgtc gccgccgcgg ccttcagcac
     2761 cgacgcgcag ccgccggacg cgctgcgcgt gaatctcggc gggccgggca cgcgcgcaca
     2821 gtgcgcggcc ggcctgcagc tcgtggccga agcactgg
//
LOCUS       DAOQJH010000375         2849 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_24141_length_2849_cov_3.084109, whole genome shotgun sequence.
ACCESSION   DAOQJH010000375 DAOQJH010000000
VERSION     DAOQJH010000375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2849)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2849)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2849
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24141_length_2849_cov_3.084109"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1882
                     /locus_tag="PLQ18_08940"
     CDS             <1..1882
                     /locus_tag="PLQ18_08940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE01818.1"
                     /translation="AADFPPDHLLEELRTRLSEPPACAPACATLEHLRLQLDPARLAI
                     TADYAAAADTLAALPLAGARVEPVAVTLDGQPAWLARAEDGAWAVRLPAGRHRVVLDV
                     PLPPELVTLDLPLVLAPAEVEVEAEGWQVSGVRNGRAGDGNLRLLRAQDDSAGETRLE
                     AGTLPPWLRVTRELVLGPADWSVETTVERLSQPGAAIGVEIPLLPGERPTGAGLRIEH
                     GRLALTLGPEDTESRWSSTLARSDTLTLAAGDGPWSEVWRLSAAPLWHVESEGTPLVH
                     RYTAGNGDWQPEWRPRPGEALKLAISRPEGVPGAVVTLDAARLTLTPGPDGALAILDL
                     RVRASRGLDHLLGLPEGALVQQVLADGRPLALSTVEGRLLLPVRPGAQELRIVWRQPG
                     ALGAHYVTPLPDPGLPGANLRVDVQVPADRWVLLTGGPRLGPAVLFWGVLAVLAAGAV
                     ALARLRFAPLGLIGWLLLAIGLAPLDPLLLLVPVGWFALCTWRERHGARLGPLAFNAL
                     QLGLALATFALLAVLVLAVQQGLLGAPDLQIAGNGSSGHLLRWYQDRSPPDWQPGTFW
                     SAPLWVYRLLMLGWALWLAWAVLGWLRWGWQCWGRDGHWRRRTAGRTAAATVWSDGNA
                     SE"
     gene            2013..2393
                     /locus_tag="PLQ18_08945"
     CDS             2013..2393
                     /locus_tag="PLQ18_08945"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNE01819.1"
                     /translation="MSNERRLFTRIPFVANVEVVIDGVPHPAELHDISLMGVLVRVDD
                     GASAALDTPVHVLLALDTERTVVIATHGRIVHNEDGRLGCRFDQVDLDSMIHLRRLME
                     LNLGDAERIKREIYEMVRLPEGQS"
     gene            complement(2535..>2849)
                     /locus_tag="PLQ18_08950"
     CDS             complement(2535..>2849)
                     /locus_tag="PLQ18_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007115378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE01820.1"
                     /translation="HGNRVEHVPDAYRRYLVNLFRKVLKLHGTPVRIEFKANVNPYEP
                     EGHVQKKQYHRPRREDREKKLAARKEAREQRKSAPATPAVPGQRPAARKPVPRKPGGR
                     KS"
BASE COUNT          318 a         1056 c         1043 g          432 t
ORIGIN      
        1 cgcggccgac ttcccgcccg atcatctgct cgaagaactg cgcacgcgcc tgtccgagcc
       61 gccggcctgt gcgcccgcct gcgcgacgct cgaacacctg cggctgcagc tcgatccggc
      121 ccgtctggcc atcaccgccg actacgcggc cgccgccgac acgctcgccg cgctgccgct
      181 cgccggcgcc cgcgtcgaac cggtcgcggt gacgctcgac ggccagccgg cctggctcgc
      241 acgcgctgag gacggtgcct gggccgtgcg tctgccggcc ggccggcatc gcgtcgtgct
      301 cgacgtgccg ctgccgcccg agctggtcac gctcgacctg ccgctcgtgc tcgccccggc
      361 cgaagtcgag gtcgaggccg agggctggca ggtcagcggc gtgcgcaacg gccgcgccgg
      421 ggacggcaac ctgcgcctgc tgcgcgcaca ggacgacagc gccggcgaaa cccggctcga
      481 agccggcacg ctgccgccgt ggctgcgcgt gacgcgcgaa ctcgtgctcg gcccggccga
      541 ctggagcgtc gagaccacgg tcgagcgcct gtcacagccc ggggcggcga tcggcgtcga
      601 gattccgctg ctgcccggcg agcgtccgac cggcgccggc ctgcgcatcg agcacggccg
      661 cctggcgctg accctcggcc ccgaggacac cgaaagccgc tggagcagca cgctcgcacg
      721 cagcgacacg ctcacgctcg ccgccggcga cgggccgtgg tccgaggtct ggcggctgtc
      781 ggccgcgccg ctctggcacg tcgaatccga gggcacgccg ctggtgcacc gctacaccgc
      841 cggcaacggc gactggcagc ccgaatggcg cccgcgtccc ggcgaggcgc tgaagctcgc
      901 gatcagccgc cccgagggcg tgcccggggc cgtggtcacg ctcgatgcgg cgcggctgac
      961 gctgacgccg gggccggacg gggcgctggc gatactcgat ctgcgcgtgc gcgccagccg
     1021 cggcctcgat cacctgctcg gcctgccgga gggggcgctg gtgcagcagg tgctcgccga
     1081 cggccggccg ctggcgctga gcacggtcga gggccggctg ctgctgccgg tgcgccccgg
     1141 cgcgcaggag ctgcgcatcg tctggcgcca gcccggtgcc ctcggtgcgc actacgtgac
     1201 gccgctgccc gatcccggcc tgcccggggc caacctgcgc gtcgatgtgc aggtgccggc
     1261 cgatcgctgg gtgctgctca ccggcgggcc gcggctcggc ccggccgtgc tgttctgggg
     1321 cgtgctggcg gtgctcgcgg ccggcgccgt ggcgctcgcg cgcctgcgct tcgcaccgct
     1381 cggcctgatc ggctggctgc tgctcgcgat cggcctcgcg ccgctcgatc cgctgctgct
     1441 gctggtgccg gtcggctggt tcgccctctg cacgtggcgc gagcgccacg gggcgcgcct
     1501 cgggccgctg gccttcaacg cgctgcagct cgggctggcg ctggcgacgt tcgcgctgct
     1561 cgcggtgctg gtgctcgccg tgcagcaggg cctgctcggt gcgccggacc tgcagatcgc
     1621 cggcaacggt tcaagcggcc atctgctgcg ctggtatcag gaccgcagcc cgccggactg
     1681 gcagcccggg acgttctggt cggcaccgct ctgggtctac cggctgctga tgctcggctg
     1741 ggcgctgtgg ctggcgtggg ccgtgctcgg ctggctgcgc tggggctggc agtgctgggg
     1801 ccgcgacggt cactggcgcc ggcgcacggc cggcaggacg gccgcagcga cggtctggag
     1861 cgacgggaat gcaagcgaat gaaacttctg acgcaagccg ccgatatcgc acttgtttcc
     1921 atgtattgca gcgcttgtac tcgcatgcct gccgggcata gcttgccggc catcccgctg
     1981 ccacacgcgg ccccaacagc caggtactcc gcatgtccaa cgaacgccgc ctgttcacgc
     2041 gcatcccttt cgtcgccaat gtcgaagtcg tgatcgacgg ggtgccgcat cccgccgagc
     2101 tgcacgacat ctcgctgatg ggcgtgctgg tgcgtgtcga cgacggcgcc agcgccgcgc
     2161 tcgacacgcc ggtgcacgtg ctgctcgcac tcgacaccga gcgcaccgtc gtcatcgcca
     2221 cgcacggcag gatcgtgcac aacgaggacg gccgcctcgg ctgccgcttc gatcaggtcg
     2281 atctcgactc gatgatccac ctgcgccggc tgatggagct gaacctcggc gacgccgagc
     2341 gcatcaagcg cgagatctac gagatggtgc gcctgccgga aggccagtcc tgacccggcc
     2401 ccgctgccgc gcccctttcc cccgccctgc cggagctgct ccggcagggc attttcttgc
     2461 gcccccggct gcaactgcgg agtctgcgca cccccggcac ccggttcgcg ggggatgcgc
     2521 cggggcgagg atgctcagct tttgcgtccg cccggcttgc gcggcaccgg cttgcgggcc
     2581 gccgggcgct ggccggggac ggccggggtg gcgggggcgg acttgcgctg ttcgcgggct
     2641 tccttgcgcg cggcgagctt cttctcgcgg tcctcgcggc gcgggcggtg gtactgcttc
     2701 ttctgcacgt ggccttcggg ttcgtagggg ttgacgttgg ccttgaactc gatgcgcacc
     2761 ggcgtgccgt gcagcttcag caccttgcgg aacaggttga cgagatagcg gcggtaggcg
     2821 tccggcacgt gctcgacgcg gttgccgtg
//
LOCUS       DAOQJH010000376         2841 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_24240_length_2841_cov_4.141062, whole genome shotgun sequence.
ACCESSION   DAOQJH010000376 DAOQJH010000000
VERSION     DAOQJH010000376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2841)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2841)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2841
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24240_length_2841_cov_4.141062"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..495)
                     /locus_tag="PLQ18_08955"
     CDS             complement(<1..495)
                     /locus_tag="PLQ18_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009111094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HNE01821.1"
                     /translation="MTAALRSDVAGGWQARLELGFAPVAARTALVHSAHRGPLRVQRP
                     FHPEADGSCHVYLLHPPGGVVGGDGLALDVQLAPGARALLTTPSASRFYRSAGARALQ
                     RQLLRVGAGARLDWLPQETIVFDGARLASTTRLELAGDAAACAWEIVCLGRPAAGEGW
                     THGEA"
     gene            complement(963..1622)
                     /gene="msrA"
                     /locus_tag="PLQ18_08960"
     CDS             complement(963..1622)
                     /gene="msrA"
                     /locus_tag="PLQ18_08960"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015956928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HNE01822.1"
                     /translation="MFGFGKKLTLPGAGEALPGREVAMRVPEKHYVNGHALRPPFPEG
                     FQQAMFGLGCFWGAERRFWQQPGVWSTAVGYAAGLTPNPGYEEVCSGRTGHAEVVLVI
                     WDPAETCYEALLKTFWEAHDPTQGMRQGNDVGTQYRSGIYAFDDAQLAAAEASKAAYQ
                     QALDARGLGTITTEIRPAPVFYYAEAYHQQYLAKNPGGYCGLGGTGVACPIGLDVTAG
                     G"
     gene            1844..2164
                     /locus_tag="PLQ18_08965"
     CDS             1844..2164
                     /locus_tag="PLQ18_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012734893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE01823.1"
                     /translation="MKLHRSLPVLPLLAALAGSGFALAPLPAGADTVIIIRDAPPPPR
                     YERRPGARPGHVWVPGHWGWYRGHHVWRAGHWVKARPHRHYVEPVWVHTPRGWRYEPG
                     YWRR"
     gene            complement(2207..>2841)
                     /locus_tag="PLQ18_08970"
     CDS             complement(2207..>2841)
                     /locus_tag="PLQ18_08970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HNE01824.1"
                     /translation="FCARRTPITIDGLMQELGCSRATVLQLTELLRDRMGAPLEHEGA
                     EYHYRRPEADDYTLPAMWLNAAELDTVLQLQALLGPIPPGPLAQALEPLRRRVARLVD
                     LQPGVSGSAWRRIRVSEPGARRVQPAHMAAIAQAVLSRLRLRLEGEFIAGEYSPQYLF
                     HCRGRWLLDAWHHGAEARRTIALEEIAAAGLLDKPAVDIEEDEPPFACLF"
BASE COUNT          404 a         1015 c         1006 g          416 t
ORIGIN      
        1 cgcctcgccg tgcgtccagc cctcgccggc cgccggccgg ccgagacaga cgatctccca
       61 ggcgcaggcg gcggcatcgc cggcgagctc cagccgcgtc gtgctcgcaa gccgcgcgcc
      121 gtcaaagacg atggtctcct gcggcagcca gtcgagccgc gccccggcgc cgacccgcag
      181 cagctgccgc tgcagcgccc gcgcgccggc actgcggtag aagcggctcg ccgaaggtgt
      241 ggtcagcagc gcccgcgcac cgggggcgag ctgcacgtcg agcgcgagcc cgtcaccgcc
      301 gaccacgccg cccggcgggt gcagcagata gacgtggcag ctgccgtccg cctcgggatg
      361 gaacggccgc tgcacgcgca gcgggccgcg atgggcgctg tgcaccagcg cggtgcgcgc
      421 tgcgaccggc gcaaagccga gttcgagccg cgcctgccag ccaccggcca catcgctgcg
      481 cagcgcggcc gtcatgcgag tcctgccgct tcagaccggt gacgggtggc accggcagcg
      541 tcaggggccg gtgggccggg gttgcggatc agggcatcgg gcatcggaca ggactcacga
      601 cagggctgcg ccggctgata cacgtttcgg gccgcccgcg acaagcccct gatccggccc
      661 ggggcgccgc cgccgtgagc ggcacagtcg atgaactttc gtttacctag tgcgaactgc
      721 accggcgcgc agatttcgcg gaattcaaaa cttaattttt attcatgtat ttatccgaga
      781 caggcactcg ggtatggatt ctgacccctg cagaccgctc ttgcgcagcg caccatgcct
      841 gcctacgctg caacgcgaca tctccggatg ttctcctcct gtggtggtat ctggccgggc
      901 ccgaaaagcc cggccttttg tttgtgcgtc agcggattac gccggactgc gcagtccgtc
      961 cgtcagccgc cggccgtcac gtccagcccg atcggacagg cgacgccggt accgcccagc
     1021 ccgcagtaac cgcccgggtt cttggccaga tactgctggt ggtaggcctc ggcgtaatag
     1081 aacaccggtg ccgggcggat ctcggtcgtg atcgtgccga ggccgcgcgc atcgagcgcc
     1141 tgctggtagg cggccttcga ggcttcggcg gctgcgagct gcgcatcatc gaaggcatag
     1201 atgccgctgc ggtactgggt gccgacatcg ttgccctggc gcatgccctg ggtcgggtcg
     1261 tgcgcttccc agaaggtctt cagcagcgcc tcgtagcagg tttcggccgg gtcccagatc
     1321 accagcacca cctcggcatg gccggtgcgg cccgagcaga cctcctcgta gcccggattc
     1381 ggcgtcaggc cggcggcgta accgaccgcg gtgctccaga cgccgggctg ctgccagaag
     1441 cggcgctcgg cgccccagaa gcagccgagg ccgaacatcg cctgctggaa gccttccggg
     1501 aacggcgggc gcagcgcgtg gccgttgacg tagtgcttct cgggcacgcg catcgcgacc
     1561 tcgcgcccgg gcagggcctc gccggcgccg ggcagggtga gcttcttgcc gaatccgaac
     1621 atgatgcagt cctccgcgga cggttcgccc cggcggggat gtccggggtc gcccttcaat
     1681 agggtccggg gcttacgcat gcaagggcgc agcggacgca ggcacggccc tgcgcgtacc
     1741 gcggacgtga cacggcgttg cgcgccggtg aaaacccgga cacccgaatc cggtcccatt
     1801 ccggtgcggt gaacgcctca ccgtgccatc gggagtcgtt gccatgaagc tccatcgaag
     1861 cctgcccgtt ctgccgctgc tcgccgcgct cgccggcagc ggcttcgcgc tcgcaccgct
     1921 gccggcgggc gcggacaccg tgatcatcat ccgcgacgcc ccgccgccgc cgcgttacga
     1981 gcggcgcccg ggcgcgcgcc ccggccacgt gtgggtgccg ggtcactggg gctggtatcg
     2041 cggtcatcac gtctggcggg ccgggcactg ggtcaaggca cgcccgcacc ggcactacgt
     2101 cgagccggtc tgggtgcata cgccgcgcgg ctggcgctac gagccgggtt actggcgccg
     2161 ctgagcggac cgggcgccac cgccgtggca ccgacgcgac gccggctcag aacagacagg
     2221 caaacggcgg ctcgtcttcc tcgatgtcga ccgccggctt gtccagcagt ccggcggccg
     2281 cgatttcttc gagcgcgatc gtgcggcgtg cctcggcgcc gtgatgccag gcatcgagca
     2341 gccagcggcc gcggcagtgg aacaggtact gcggcgagta ttcgccggcg atgaactcgc
     2401 cttccaggcg caggcgcagg cgcgacagca cggcctgggc gatggcggcc atgtgcgcgg
     2461 gctgcacgcg ccgggcgccc ggctcgctga cgcggatgcg ccgccacgcc gaaccgctca
     2521 cgccgggctg caggtcgacc agccgcgcca cccgccggcg cagcggctcc agcgcctgcg
     2581 ccagcggtcc gggcggaatc ggcccgagca gcgcctgcaa ctgcagcacg gtgtcgagtt
     2641 cggcggcgtt cagccacatc gccggcagcg tgtagtcgtc ggcctcgggg cggcggtagt
     2701 gatactcggc gccctcgtgt tcgagcgggg cacccatgcg atcgcgcagc agttcggtca
     2761 gttgcagcac ggtcgcgcgg ctgcagccga gttcctgcat cagtccgtcg atggtgatcg
     2821 gcgtgcggcg tgcacaaaac a
//
LOCUS       DAOQJH010000377         2841 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_24242_length_2841_cov_3.559225, whole genome shotgun sequence.
ACCESSION   DAOQJH010000377 DAOQJH010000000
VERSION     DAOQJH010000377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2841)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2841)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2841
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24242_length_2841_cov_3.559225"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(549..1151)
                     /locus_tag="PLQ18_08975"
     CDS             complement(549..1151)
                     /locus_tag="PLQ18_08975"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNE01825.1"
                     /translation="MQTHKYRPLKFRHLDTGVTCFRPRQHLPDRITQDSPATDAMTDL
                     RQVSAQMVSLQTPMNVALERMIKGGVRLLLVADPDGTIRGVITSRDIQGEKPMKLMEK
                     TGTKREELTVRDIMTLYPRLEVLDITDVLRATVGDIIATLREQGRQHALVVDTDPDSG
                     KEAVRGIFSLSQIGQQLGLEIHPQARPTTFAEIESALMRG"
     gene            complement(1286..1798)
                     /locus_tag="PLQ18_08980"
     CDS             complement(1286..1798)
                     /locus_tag="PLQ18_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016352675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNE01826.1"
                     /translation="MDQQQTSNPARGGRRILSCGVIVLRASAGGSGGNWEYLLLRAFN
                     YWDFPKGMREPGEHPLSTALREVREETTIDSLDFRWGEVFRETPPYNHGCKVARYYLA
                     VAPADATVDLPVSPELGRPEHSEFRWVTRREAWSLLTPRVRAILRWSDTILSRPPRQD
                     APAPLPSPAD"
     gene            complement(1815..>2841)
                     /gene="hemE"
                     /locus_tag="PLQ18_08985"
     CDS             complement(1815..>2841)
                     /gene="hemE"
                     /locus_tag="PLQ18_08985"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020503389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HNE01827.1"
                     /translation="VDATPVWMMRQAGRYLPEYRATRARAGSFMSLCTAPELACEVTL
                     QPLERFPLDAAILFSDILTVPDAMGLGLSFAEGEGPRFAHPVRSAAEIERLGVPDPEL
                     ELGYVMDAVRLIRRELAGRVPLIGFSGSPWTLATYMVEGGSSKEFAKVKGLMYEQPQV
                     LHKLLGTVTRAVIAYLNAQIGAGAQAVMVFDTWGGALSPATYRSFSLDYMARIVEGLN
                     REAEGRRVPVILFTKGGGAWLEAMADTGCDALGLDWTTDIGEARARVGSRVALQGNLD
                     PCVLYASPERIRAEVGQVLAGYGRGSGHVFNLGHGIHPHVDPEHAGAMVAAVHELSRA
                     YHGGQTD"
BASE COUNT          421 a          917 c         1034 g          469 t
ORIGIN      
        1 aaaaaccatg cgattacgtg tgatcggtat cagcccctcg ccgcccgtgc gggtgggggg
       61 gcggaaccgg tgacgggttc tgccgtgacc ggcacccggt gcgcacaacc gcggtgccgg
      121 tcgacgagac tgcggttgcg gtcgatggaa gcgcgggtca cacgctcagg tgggctgttt
      181 aatcagatta aaccgtgggt cacacgctca ggtgtgctgt ttaatcagat taaaccgcgg
      241 ttcacacgct cgggagtgct gtttaatcag attaaaccgc gggtcatacg ctcgggcgtg
      301 ctgtttaatc agattaaacc gcgggtcaca cgctcaggcg tgctgttcaa tcggattaaa
      361 ccgtgggtca cacgctcggg tgtgccgttc gcgtcgatgg cagcgcggtt cgcgcgggcg
      421 ccgtgcgctt gcggtcgata cgcccgaaac ggcgtgcttc gcagccgcag gagccggaaa
      481 acagcaaggg cgcctcgcgg cgcccttgtc gtttgtatcg gctgaacagc ggggaagatc
      541 gcggcgcctc agccgcgcat cagcgcgctc tcgatctcgg cgaaggtcgt cggtcgcgcc
      601 tgcgggtgga tttcgaggcc gagctgctgg ccgatctgcg acagcgagaa gatgccgcgc
      661 accgcctcct tgccgctgtc cggatcggtg tcgaccacca gcgcgtgctg gcggccctgc
      721 tcgcgcagcg tcgcgatgat gtcgccgacc gtggcgcgca gcacgtcggt gatgtcgagc
      781 acttcgaggc gcgggtacag cgtcatgatg tcgcgtacgg tcagttcctc gcgcttcgtg
      841 ccggtcttct ccatcagctt catcggcttc tcgccctgga tgtcgcggct ggtgatgacg
      901 ccgcggatcg tgccgtccgg gtcggccacc agcagcaggc gcacgccgcc cttgatcatg
      961 cgttccagcg cgacgttcat cggcgtctgc agcgacacca tctgggcgct gacctgacgc
     1021 aggtcggtca tcgcgtcggt cgccgggctg tcctgcgtga tgcgatcggg cagatgctgg
     1081 cgcgggcgga agcaggtgac cccggtgtcg aggtggcgga atttcagcgg gcggtacttg
     1141 tgagtctgca tgatggcggg cctccggggc ggtggtcttg aactgcctgg gcttcgttct
     1201 tggggacgcg ggccataggc atagacgcgg gggccggaac tgtaaatcgg cccgaccgcg
     1261 ccgcagggcg ggcttaatgt gaatttcagt cggcgggcga cggcagcggc gcgggcgcgt
     1321 cctggcgcgg cggacggctc aggatggtgt cggaccagcg caggatcgcg cgcacgcgcg
     1381 gcgtcagcag gctccaggcc tcgcgccgcg tcacccagcg gaattcgctg tgctcgggcc
     1441 ggccgagctc ggggctgacc ggcaggtcga ccgtggcatc ggccggggcc acggccaggt
     1501 aatagcgcgc gaccttgcag ccgtggttgt acggcggcgt ctcgcggaac acctcgcccc
     1561 agcggaaatc gagcgagtcg atcgtggttt cctcgcgcac ctcgcgcagc gccgtgctga
     1621 gcgggtgctc gccgggctcg cgcatgccct tcgggaagtc ccagtagttg aaggcgcgca
     1681 gcagcagata ttcccagttg ccgccgctgc cgccggcgga cgcgcgcagc acgatgacgc
     1741 cgcaggacag gatgcggcgg ccgccacggg ccgggttcga ggtctgctgc tggtccacga
     1801 cgctccctgt cgtctcagtc ggtctggccg ccgtgatagg cgcgcgacag ttcgtggacc
     1861 gcggcgacca tcgcgccggc gtgttccgga tcgacgtgcg ggtggatgcc gtggccgagg
     1921 ttgaagacgt gaccgctgcc gcgaccgtag ccggcgagca cctgaccgac ctcggcacgg
     1981 atgcgctcgg gcgaggcgta cagcacgcag ggatcgaggt tgccctgcag cgccacgcgg
     2041 ctgccgacgc gggcacgggc ctcgccgatg tcggtggtcc agtcgaggcc gagcgcatcg
     2101 cagccggtgt cggccatcgc ttcgagccag gctccgccgc ccttcgtgaa caggatcacc
     2161 ggcacgcgcc ggccttcggc ctcgcggttg aggccttcga cgatgcgcgc catgtagtcg
     2221 agcgagaagc tgcggtaggt ggccggcgac agcgcaccgc cccaggtgtc gaacaccatc
     2281 accgcctgcg caccggcgcc gatctgcgcg ttcaggtagg cgatgaccgc gcgcgtgacc
     2341 gtgccgagca gcttgtgcag cacttgcggc tgttcgtaca tcaggccctt gaccttcgcg
     2401 aactccttgc tggagccgcc ttcgaccatg taggtcgcga gcgtccacgg gctgccggag
     2461 aagccgatca gcggcacgcg accggcgagt tcgcggcgga tcaggcgcac ggcgtccatc
     2521 acgtagccga gttcgagttc gggatcgggc acgccgaggc gttcgatctc ggcggcgctg
     2581 cgcaccgggt gcgcgaagcg cggcccctcg ccctcggcga aggacaggcc gagccccatc
     2641 gcatcgggca cggtcaggat gtcggagaac aggatcgccg catcgagcgg gaagcgctcg
     2701 agcggctgca gcgtgacctc gcaggccagc tccggagcgg tgcagagcga catgaagctg
     2761 ccggcgcgcg cgcgcgtggc gcggtactcg ggcaggtagc ggccggcctg gcgcatcatc
     2821 cagaccggcg tcgcatcgac g
//
LOCUS       DAOQJH010000378         2840 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_24257_length_2840_cov_2.598564, whole genome shotgun sequence.
ACCESSION   DAOQJH010000378 DAOQJH010000000
VERSION     DAOQJH010000378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2840)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2840)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2840
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24257_length_2840_cov_2.598564"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            57..476
                     /locus_tag="PLQ18_08990"
     CDS             57..476
                     /locus_tag="PLQ18_08990"
                     /inference="COORDINATES: protein motif:HMM:NF037928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host attachment protein"
                     /protein_id="HNE01828.1"
                     /translation="MTATWILVADASRARLFSVSKSIGPLQEIGSFDHPGARAKTRDL
                     LADGQCSGNGSGDRECAEPRRHEALNFARELSEHLRLSRVQGLFDRLYIAAAPAFLGL
                     LRGRLDSPTAQLVIDEVSKDLTQLAPDAIRRHLPERL"
     gene            562..1998
                     /gene="gltX"
                     /locus_tag="PLQ18_08995"
     CDS             562..1998
                     /gene="gltX"
                     /locus_tag="PLQ18_08995"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018938723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HNE01829.1"
                     /translation="MQRFPAAGPIATMVKTRFAPSPTGFLHIGGARTALYSWLHARRH
                     GGQFVLRIEDTDLERSTPEAVAAILDGMAWLGLDHDEGPYFQTQRFGRYREVIAQMLA
                     AGTAYRCYCTKEELDALRAEQMAQKIKPRYDGRYRDYDGPPRPGVEPVIRFKNPLDGE
                     VVIDDLVHGPITIRNDELDDLVIARADGTPTYNFCVVVDDMDMGITHVIRGDDHINNT
                     PRQINILRALGATVPSYAHVAMILGDDGQKLSKRHGAVSVLQYRDEGFLPGAVLNYLV
                     RLGWSHGDQEVFTREEMIRLFDIGDVNKAASAFNTSKLLWLNQQYLMSTPPEQIAWLL
                     RPFLSALGIDPDSGPDPVEVVREMACRAQTLQELAAQSAFFWQDFEAYDEKSARQHLK
                     PEAAQPLACLRERLAALPAWEPAALHETVQAVATELELKMGKVAQPLRVAVVGRAASP
                     GIDVTLALTGREAVLRRLDRALAHLAAA"
     gene            2051..>2840
                     /locus_tag="PLQ18_09000"
     CDS             2051..>2840
                     /locus_tag="PLQ18_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005995390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HNE01830.1"
                     /translation="MSTPPNHFIRQIIDEDLAAGRLPAAGLRTRFPPEPNGYLHIGHA
                     KSICLNFGLARDYGGQCNLRFDDTNPCKEEVEFVEAIARDVRWLGFDWDSRQFYASDY
                     FERLYACAEALIGKGLAYVDDLTAEQIREYRGTLTEPGRDSPYRTRSAAENLDLFRRM
                     RAGEFADGTRVLRAKIDMASPNINLRDPAIYRIRHEHHHQTGDAWCIYPMYDFAHALS
                     DAFEGITHSICTLEFEDHRPLYDWCVANCPVEATPHQYEFSRLTL"
BASE COUNT          457 a         1070 c          907 g          406 t
ORIGIN      
        1 gcgtgcgaag gccagcggca tcccgggccc tccggtccgg gtttgtggag gtgagcatga
       61 ccgcaacgtg gatactggtg gcggacgcaa gccgcgcccg gctgttcagc gtcagcaagt
      121 ccatcggccc gctgcaggaa atcggcagct tcgaccaccc cggcgcgcgc gccaagaccc
      181 gtgacctgct cgccgacggc cagtgttccg gcaacggcag cggggatcgc gagtgtgccg
      241 agccgcggcg ccacgaggcg ctgaacttcg cgcgcgagct gtccgagcac ctgcgcctca
      301 gtcgcgtgca gggtctgttc gaccggctct acatcgccgc ggccccggcc ttcctcggcc
      361 tgctgcgcgg ccggctcgac agcccgaccg cgcagctggt gatcgacgag gtcagcaagg
      421 acctcaccca gctcgcgccc gacgcgatcc gccggcacct gcccgaacgg ctctgagctt
      481 tcctgcctgc gctgcggggg agaccggacg gtctcccccg cagcgtttcc ggcccccccg
      541 ccggccgccc ggatcgctag aatgcagcgc tttcccgctg ccggaccgat cgcgacgatg
      601 gtcaagaccc gcttcgcccc cagccccacc ggcttcctgc acatcggcgg tgcgcgtacc
      661 gcgctgtact cgtggctgca tgcccgccgc cacggcggcc agttcgtgct gcgcatcgag
      721 gacaccgatc tcgaacgctc gacgcccgag gccgtcgccg cgatcctcga cggcatggcc
      781 tggctcggcc tcgatcacga cgaaggcccg tacttccaga cgcagcgctt cgggcgctac
      841 cgcgaggtca tcgcgcagat gctcgcggcc ggcaccgcct accgctgcta ctgcacgaag
      901 gaagaactcg acgcgctgcg cgccgagcag atggcgcaga agatcaagcc gcgctacgac
      961 ggccgctacc gcgactacga cggcccgccg cgccccggcg tcgagccggt gatccgcttc
     1021 aaaaacccgc tcgatggcga ggtcgtcatc gacgatctcg tgcacgggcc gatcacgatc
     1081 cgcaacgacg aactcgacga cctggtcatc gcccgcgccg acggcacgcc gacctacaac
     1141 ttctgcgtcg tcgtcgacga catggacatg ggcatcacgc acgtgatccg cggcgatgac
     1201 cacatcaaca acacgccgcg ccagatcaac atcctgcgcg cactcggcgc gacggtgccg
     1261 agctacgcgc acgtggcgat gatcctcggc gacgatggcc agaagctctc gaagcgccac
     1321 ggcgcggtca gcgtgctgca gtaccgcgac gagggcttcc tgcccggtgc agtgctcaac
     1381 tacctcgtgc gcctcggctg gtcgcacggc gatcaggaag tcttcacgcg cgaggagatg
     1441 atccgcctgt tcgacatcgg cgatgtcaac aaggccgcct cggcgttcaa cacctcgaag
     1501 ctgctgtggc tgaaccagca gtacctgatg agcacgccgc ccgagcagat cgcctggctg
     1561 ctgcggccct tcctctcggc gctcggcatc gacccggaca gcggtcccga cccggtcgag
     1621 gtcgtgcgcg agatggcctg ccgcgcgcag acgctgcagg aactcgccgc gcagagcgcc
     1681 ttcttctggc aggatttcga ggcttacgac gagaaatccg caagacagca cctgaagccc
     1741 gaagctgcgc agccgctcgc ctgcctgcgc gagcgcctgg ccgcgctgcc ggcctgggag
     1801 ccggccgcgc tgcacgagac cgtgcaggcg gtcgcgaccg aactggaact caagatgggc
     1861 aaggtcgcgc agccactgcg cgtcgccgtc gtcgggcgcg ccgcctcgcc cggcatcgac
     1921 gtgacgctgg cgctgaccgg ccgcgaggcc gtgctgcgcc gcctcgaccg cgcgctcgcg
     1981 catcttgccg ccgcctgaac cttccgccgc cccgccgcgg aacctgtctg atcagttgct
     2041 ggacgccgcc atgagcaccc cgccgaacca tttcatccgt cagatcatcg acgaggacct
     2101 cgccgccggc cggctgccgg ccgccggcct gcgcacgcgc tttccgcccg agccgaacgg
     2161 ctacctgcac atcggccacg ccaagtcgat ctgcctgaac ttcggcctgg cgcgcgacta
     2221 cggcggccag tgcaacctgc gcttcgacga caccaatccg tgcaaggagg aggtcgagtt
     2281 cgtcgaggcg atcgcgcgcg acgtgcgctg gctcggcttc gactgggaca gccgccagtt
     2341 ctacgcctcg gactacttcg agcggctgta cgcctgcgcc gaggcgctga tcggcaaggg
     2401 actcgcctac gtcgatgacc tcacggccga gcagatccgg gaataccgcg gcacgctgac
     2461 cgagcccggc cgcgacagcc cgtaccgcac gcgcagcgcc gcggagaacc tcgacctgtt
     2521 ccggcgcatg cgcgctggcg agttcgccga cggcacgcgc gtgctgcgcg cgaagatcga
     2581 catggcctcg ccgaacatca acctgcgcga cccggcgatc taccgcatcc gccacgagca
     2641 ccaccaccag accggcgatg cctggtgcat ctacccgatg tacgacttcg cgcacgcgct
     2701 gtcggacgcc ttcgagggca tcacgcactc gatctgcacg ctggagttcg aggaccaccg
     2761 gccgctgtac gactggtgcg tcgccaactg cccggtcgag gcgacgccgc accagtacga
     2821 gttctcgcgc ctgacgctgc
//
LOCUS       DAOQJH010000379         2835 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_24323_length_2835_cov_4.560791, whole genome shotgun sequence.
ACCESSION   DAOQJH010000379 DAOQJH010000000
VERSION     DAOQJH010000379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2835)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2835)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2835
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24323_length_2835_cov_4.560791"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..437)
                     /locus_tag="PLQ18_09005"
     CDS             complement(<1..437)
                     /locus_tag="PLQ18_09005"
                     /inference="COORDINATES: protein motif:HMM:NF028500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE01831.1"
                     /translation="MTHPLAEAGRSVSASRSLDALKGVGPKLAGRLAKLGLRTVEDLL
                     FHLPLRWQDRTQVVPISELTIGEPAVIEAEVLGMETVGRTRQSFLVRVGDGSGTLVLR
                     FFHFGASLQAAMARGARLRLFGEVRSGPTGPEIIHPEHRPADAP"
     gene            complement(462..842)
                     /locus_tag="PLQ18_09010"
     CDS             complement(462..842)
                     /locus_tag="PLQ18_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016968788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HNE01832.1"
                     /translation="MAESLIEYEDACEEVRAVYDDIMRTRKVEHVNNFWKAIAHHPPT
                     LARTWEAMKAVMADDGGLDPLMKELIYIAVSVANGCDYCIASHTAAARRKGMTEAMHG
                     ELLAVIGMASATNALATAHRVPID"
     gene            complement(982..1719)
                     /locus_tag="PLQ18_09015"
     CDS             complement(982..1719)
                     /locus_tag="PLQ18_09015"
                     /inference="COORDINATES: protein motif:HMM:NF021515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2242 domain-containing protein"
                     /protein_id="HNE01833.1"
                     /translation="MSSVPAVSALLPAVAGRLLCAAVLAATAACSGPGSGQAVYKNEQ
                     FAASSDYSHRYAAPPVATCEAARRALLSQGYVIVDAKPEAIEARKTFQPDRENHTELN
                     FRITCAGDGDVDSDGFADAALLFANAEQTLYTVKKTSSSASVGVGVVGSISLPIGQSQ
                     DSLIKIGAETVPAGPFYQRFFALVQQYLGSSRPKPRPAPVTPPVAPPPPAAPAALPPV
                     PAPALPPGSAPVPPLPAPASAAPLTPT"
     gene            complement(1746..>2835)
                     /locus_tag="PLQ18_09020"
     CDS             complement(1746..>2835)
                     /locus_tag="PLQ18_09020"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009540384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase class II"
                     /protein_id="HNE01834.1"
                     /translation="LQMAVVLTYGAGLPIVKLGRMAGQFAKPRSAPVETRGDVTLPSY
                     RGDNVNGLEFTAAARAPDPQRQVQAYFQSAATMNLLRAFAQGGYADLHQVNRWTADFI
                     AASAQGDRYRELADHIGRTLKFMEACGLNGDNTPQIRETAFYTSHEALLLGYEQALTR
                     RDSLTGDWYDCSAHLLWIGERTRQPDGAHIEFCRGIGNPVGVKVGPKTKPGDVLRLCD
                     LLNPANEAGRLMLIARMGADTVGDALPALVRAVQAEGRKVVWSCDPMHGNTITSSTGY
                     KTRPFERVLAEVRGFFAVHRAEGTHAGGVHFEMTGQNVTECTGGAEGIDDEALAACYL
                     THCDPRLNGSQSLELAFLIAEMLKAERA"
BASE COUNT          381 a          990 c         1041 g          423 t
ORIGIN      
        1 ggcgcatcgg ccgggcggtg ctcgggatgg atgatttccg ggccggtcgg gccgctgcgc
       61 acctcgccga acagccgcag ccgcgcgccg cgcgccatcg ccgcctgcag cgaggcaccg
      121 aagtggaaga agcgcagcac cagcgtgccg ctgccatcgc cgacgcgcac caggaaggac
      181 tggcgcgtgc gcccgaccgt ctccatgccg agcacctcgg cctcgatcac ggccggctcg
      241 ccgatcgtga gttcgctgat cggcacgacc tgcgtgcggt cctgccagcg cagcggcagg
      301 tgaaacagca gatcctcgac cgtgcgcagg ccgagcttcg ccagccgccc ggcgagcttg
      361 gggccgacgc ccttcagcgc gtccagcgag cggctggcgg agacgctgcg accggcctcg
      421 gcgagcggat gcgtcatgcg ggcacgatcc cggagaccgg ttcagtcgat cggcacgcgg
      481 tgcgcggtgg cgagcgcgtt ggtggcgctc gccatgccga tcaccgccag cagctcgccg
      541 tgcatggcct cggtcatgcc cttgcgccgt gccgccgccg tgtgcgaggc gatgcagtag
      601 tcgcagccgt tggcgacgct gaccgcgatg tagatcagtt ccttcatcag cggatcgagc
      661 ccgccgtcat cggccatcac cgccttcatg gcctcccagg tgcgcgccag cgtcggcgga
      721 tggtgcgcga tcgccttcca gaagttgttg acgtgctcga ccttgcgcgt gcgcatgatg
      781 tcgtcgtaga cggcacgcac ctcctcgcag gcgtcctcgt actcgatcag ggattcggcc
      841 atggttccgg tcctcgggct gcacgggggc cggccgcgca ggcgcggcgg ccgggacggg
      901 cgatgcggcc ggacgccctg ccccgggcgc ccgctcatcg atcatgcacc gccggcagcg
      961 caacggtcag ccccgctgcg ctcaggtcgg cgtcagcggt gcggcgctgg ccggcgcggg
     1021 cagcggcggc accggcgccg atcccggcgg cagggccggt gccgggaccg gcggaagcgc
     1081 ggccggcgcg gccggcggag gtggtgcgac cggcggcgtc accggcgccg gtcgcggctt
     1141 cgggcggctg ctgccgagat actgctgcac cagcgcgaag aagcgctggt agaacggccc
     1201 ggccggcacg gtctcggcac cgatcttgat cagcgaatcc tgactctggc cgatcggcag
     1261 cgagatcgaa ccgaccacgc cgacgccgac gctggccgag gagctggtct tcttgaccgt
     1321 gtacagcgtc tgctcggcgt tcgcgaacag cagcgcggca tcggcaaagc catcgctgtc
     1381 gacatcgccg tcgccggcgc aggtgatgcg gaagttcagc tcggtgtggt tctcgcgatc
     1441 gggctggaag gtcttgcggg cctcgatcgc ctcgggcttc gcatcgacga tcacgtaacc
     1501 ttggctgagc agcgcgcggc gcgcggcctc gcaggtcgcg accggaggtg ccgcgtagcg
     1561 gtgcgaatag tcggacgagg ccgcgaactg ttcgttcttg tagacggcct ggccgctgcc
     1621 gggcccgctg caggccgcgg tggcggcgag cacggcggcg cagagaagcc ggccggcgac
     1681 ggccggcagc agcgcggata cggcgggtac ggacgacatc ggctcctcac cggctccggg
     1741 cgcgctcacg cgcgttcggc cttcagcatc tcggcgatca ggaatgcgag ttccagcgac
     1801 tgcgagccgt tcaggcgcgg atcgcagtgc gtcagatagc aggcggccag cgcctcgtcg
     1861 tcgatgcctt cggcgccacc ggtgcattcg gtgacgttct gcccggtcat ctcgaaatgc
     1921 acgccgccgg cgtgcgtgcc ctcggcgcgg tgcacggcga agaagccgcg cacctcggcg
     1981 agcacgcgct cgaacggccg ggtcttgtag ccggtgctcg acgtgatcgt gttgccgtgc
     2041 atcgggtcgc aggaccagac caccttgcgc ccttccgcct gcaccgcgcg caccagcgcc
     2101 ggcagcgcgt cgccgaccgt gtcggcgccc atgcgcgcga tcagcatcag ccggccggcc
     2161 tcgttggcgg ggttgagcag gtcgcagagg cgcagcacgt cgccgggctt cgtcttcggg
     2221 ccgaccttga cgccgaccgg gttgccgatg ccgcggcaga actcgatgtg cgcgccatcg
     2281 ggctggcgcg tgcgttcgcc gatccacagc aggtgcgccg agcagtcgta ccagtcgccg
     2341 gtcaggctgt cgcgccgggt cagcgcctgc tcgtagccga gcagcagggc ctcgtgcgag
     2401 gtgtagaacg cggtctcgcg gatctgcggc gtgttgtcac cgttcaggcc gcaggcctcc
     2461 atgaacttca gcgtgcggcc gatgtgatcg gcgagctcgc ggtagcggtc gccctgcgca
     2521 ctggcggcga tgaagtccgc cgtccagcgg ttgacctggt gcaggtcggc atagccgccc
     2581 tgcgcgaagg cgcgcagcag gttcatggtc gcggccgact ggaagtaggc ctgcacctga
     2641 cgctgcgggt ccggcgcgcg cgctgcggcc gtgaattcga ggccgttgac gttgtcgccg
     2701 cggtagctcg gcagcgtcac gtcgccgcgc gtctcgaccg gcgcactgcg cggcttcgcg
     2761 aactgcccgg ccatgcgccc gagcttcacg atcggcaggc cggcgccgta ggtcagcacc
     2821 acggccatct gcagc
//
LOCUS       DAOQJH010000380         2828 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_24433_length_2828_cov_3.812117, whole genome shotgun sequence.
ACCESSION   DAOQJH010000380 DAOQJH010000000
VERSION     DAOQJH010000380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2828)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2828)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2828
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24433_length_2828_cov_3.812117"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(125..499)
                     /locus_tag="PLQ18_09025"
     CDS             complement(125..499)
                     /locus_tag="PLQ18_09025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE01835.1"
                     /translation="MNTPFNTLFPKLFLVALAGLPAAVLAHGDAATGAHGGTLIESGE
                     VVLEWIGARNTICLSDHGSGAPIPSAQAGGRIIVAGAGAQELTPADGNCLRYPTALAA
                     GSKAVVSIRLPGRPPVQARLEP"
     gene            complement(604..>2828)
                     /locus_tag="PLQ18_09030"
     CDS             complement(604..>2828)
                     /locus_tag="PLQ18_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNE01836.1"
                     /translation="SLCYHLPVAQRLFEEPGARALYLFPTRALARDQEHALASLVRAA
                     GLGAAVATYDGDTAADARRAIRERAAVVITNPDMLHAGVLPHHAAWARFFASLRYVVV
                     DELHAYTGVFGSHVANVLRRLRQNRALAEPRPALAVIAQATLEVRSAIFYATLIIVLV
                     FVPLFALAGIEGRLFTPLGIAYMVSMLASMLVSVTVTPVLCHYLLARGGVLEHGDSPL
                     VAWLKRQDARLLGWSFRHTQGLALAAVGLVLAAGYGLTQLPRAFLPAFNEGSYTVSLL
                     LNPGTSLAESNRIGTLAERLLLSVPEVVHTGRRTGRAELDEHAEGVHSSEIDVDLKPS
                     ERPRAVVLAELRARLALLPAAVNIGQPISHRLDHLLSGVRAQIALKLFGDDPDTLRGL
                     AAGLEEKLRTLPGLVDVQLEKQVPIPQVQVQLDYARAARYGVTPAALGEALETLVNGR
                     VVTQLIERERRYELVLRLAEAERTPQRLGELLVGTPGGWIPLRLVADIIETVGPNQIA
                     RDDGRRRIVISANGDGRDMAAIVADLRALLAATPLPEGYHTTLEGQFQAQESATRLIA
                     GLALAAVGLIFVVLYARYRAVVFALLIMANIPLATVGGVAALWLAGQVLSVASLVGFI
                     TLAGISTRNGILKVSHYLNLMLHEGEDFSDAMIIRGSLERLTPVLMTALVAATGLVPL
                     LLTPDAPGREILYPVAVVIFGGLISSTVLDTILTPVLFRRFGRAAALRLAAAGGEERY
                     "
BASE COUNT          414 a          993 c         1068 g          353 t
ORIGIN      
        1 ctcccccggc ccctctcccg caaggggaga ggggagatcg acgcgcggtc gttggtggtc
       61 gatccatcct gtccgcggaa ccgctgactt gtgtagatac ccatgcccgc cggggcaggc
      121 gggctcacgg ttccagccgc gcctgcaccg gcggccgccc cggcagccgg atcgagacga
      181 cggccttgct gccggcggcg agcgccgtcg gatagcgcag gcagttgccg tcggccggcg
      241 tgagttcctg cgcgccggcg ccggcgacga tgatccggcc accggcctgc gcgctcggga
      301 tcggggcgcc gctgccgtga tcgctgaggc agatggtgtt gcgcgcgccg atccattcga
      361 gcacgacctc gccggactcg atcagcgtgc cgccgtgcgc gccggtggcg gcgtcgccgt
      421 gggcgaggac ggccgccggc aggccggcca gcgcgacgag gaacagcttc ggaaacaggg
      481 tgttaaacgg cgtattcatg gcttcggaac tccggatcag tcggggggat gcaggcgggc
      541 agcgcgcagg gccggggccg gccggcgagg cgcggatcgc cggcagccgg ccgccggtgc
      601 ccatcagtag cgctcctcgc caccggccgc cgcgagcctc agcgccgccg cgcggccgaa
      661 gcggcggaac agcaccggcg tcaggatcgt gtcgagcacg gtcgagctga tcaggccgcc
      721 gaagatcacg accgcgaccg ggtacaggat ctcgcgcccc ggtgcgtccg gcgtcagcag
      781 cagcggcacc aggccggtgg ccgcgaccag cgccgtcatc agcaccggcg tcaggcgttc
      841 gaggctgccg cggatgatca tcgcgtcgct gaagtcctcg ccctcgtgca gcatcaggtt
      901 caggtagtgg ctgaccttca ggatgccgtt gcgcgtgctg atgccggcga gcgtgatgaa
      961 gccgaccaga ctcgcgaccg acagcacctg cccggccagc cacagcgccg ccacgccgcc
     1021 gacggtcgcg agcgggatgt tggccatgat cagcagcgcg aacaccaccg cgcggtagcg
     1081 cgcgtacagc acgacgaaga tcagcccgac cgcggcgagc gccagcccgg cgatcagccg
     1141 cgtcgccgat tcctgggcct ggaactggcc ttcgagcgtc gtgtggtagc cctcgggcag
     1201 cggcgttgcc gccagcagcg cgcgcaggtc ggcgacgatc gccgccatgt cgcggccatc
     1261 gccgttggcc gagatcacga tgcgccggcg gccgtcgtcg cgggcgatct ggttcgggcc
     1321 gacggtctcg atgatgtcgg cgaccaggcg cagcggaatc cagccgcccg gcgtgccgac
     1381 cagcagctcg ccgaggcgct gcggcgtgcg ctcggcctcg gccagacgca gcaccagctc
     1441 gtagcgccgt tcgcgctcga tcagctgcgt gacgacgcgg ccgttgacca gcgtctccag
     1501 cgcctcgccg agcgcggccg gggtcacgcc gtagcgtgcg gcgcgcgcgt agtcgagctg
     1561 cacctgcacc tgcgggatcg gcacctgctt ctcgagctgc acgtcgacga ggccgggcag
     1621 cgtgcgcagt ttttcctcca gcccggcggc gaggccgcgc agcgtgtccg ggtcatcgcc
     1681 gaacagcttc agcgcgatct gcgcgcgcac gcccgagagc aggtgatcga gccggtgcga
     1741 gatcggctgg ccgatgttga ccgccgccgg cagcagcgcg agacgcgcgc gcagctcggc
     1801 gagcacgacg gcgcgcggac gttccgaagg cttcaggtcg acatcgatct cgctcgaatg
     1861 cacgccctcg gcgtgctcgt cgagttcggc gcggccggtg cgccggccgg tgtgcacgac
     1921 ctcgggcacc gacagcagca ggcgctcggc cagcgtgccg atgcggttcg actcggcgag
     1981 cgaggtgccg gggttcagca gcaggctgac cgtgtagctg ccctcgttga aggccggcag
     2041 gaaggcacgc ggcagctgcg tgaggccgta gccggccgcg agcaccagcc cgaccgcggc
     2101 cagcgccagc ccctgcgtgt gccggaacga ccagccgagc aggcgcgcat cctggcgctt
     2161 cagccaggcc accagcgggc tgtcaccgtg ttcgagcacg cccccgcgcg cgagcaggta
     2221 gtggcagagc accggcgtca ccgtcaccga caccagcatg ctcgcgagca tcgacaccat
     2281 gtaggcgatg ccgagcggcg tgaacagccg cccctcgatg ccggcgagcg cgaacagcgg
     2341 cacgaacacc agcacgatga tcagcgtcgc gtagaagatc gccgagcgca cctcgagcgt
     2401 cgcctgcgcg atcaccgcga gcgccggacg cggttcggcg agcgcccggt tctggcgcag
     2461 ccgccgcagc acgttggcga cgtgcgagcc gaagaccccc gtgtaggcgt ggagctcgtc
     2521 gacgacgacg tagcggagcg acgcgaagaa gcgcgcccac gccgcgtggt gggggagcac
     2581 gccggcgtgg agcatgtcgg ggttggtgat gacgaccgcg gcgcgctcgc ggatggcgcg
     2641 gcgcgcgtcg gcggcggtgt ctccgtcgta ggtggccacg gcggcgccga gccccgccgc
     2701 gcggacgagc gacgcgagcg cgtgctcctg gtcgcgggcg agggcgcgcg tggggaagag
     2761 gtagagggcg cgcgcgccgg gctcttcgaa gagccgctgc gcgacgggga ggtggtagca
     2821 gagcgact
//
LOCUS       DAOQJH010000381         2826 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_24469_length_2826_cov_4.822086, whole genome shotgun sequence.
ACCESSION   DAOQJH010000381 DAOQJH010000000
VERSION     DAOQJH010000381.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2826
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24469_length_2826_cov_4.822086"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1179)
                     /gene="leuS"
                     /locus_tag="PLQ18_09035"
     CDS             complement(<1..1179)
                     /gene="leuS"
                     /locus_tag="PLQ18_09035"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019333145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HNE01837.1"
                     /translation="MEESYQPQAVEAAAQRYWNEQRSFEVAADPAREKFYCLSMFPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGRNVVQPMGWDAFGMPAENAAIKNSVAPARWTY
                     ENIDYMRAQLKSLGLGIDWSRELATCTPDYYRWEQWLFTRLFEKGVIYRRNGVVNWDP
                     VDQTVLANEQVIDGRGWRSGALVERREIPMYYFRITQYADELLAALDDLPGWPEQVKT
                     MQRNWIGKSRGMDVVFPRVGADGGVKVYTTRPDTLMGATYVAVAAEHPLALEAAAGNP
                     ELAAFIDECRKGGVAEADIATQEKRGLPTGLCVRHPFTGRELPVWVANYVLMGYGEGA
                     VMAVPAHDERDFEFAHRYGLPVEPVIRTPAGDATPLPWQPAYAEHGTLIHSGEFDGLD
                     F"
     gene            1336..1851
                     /locus_tag="PLQ18_09040"
     CDS             1336..1851
                     /locus_tag="PLQ18_09040"
                     /inference="COORDINATES: protein motif:HMM:NF018946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HNE01838.1"
                     /translation="MTEPRHRLAEAYARLLERLHLHDADRDASEATPPLRARIDRAAA
                     DAHHLGELTKEEAGLLAHWARRDLADAGRHLAETGQELGSWLRFDTELVEERLLDWFG
                     RAADQSRLELLEFADTLERLSHYETGEVTGPGTLACEACGERIEFRGTGTIPSCPKCG
                     HTRFERPVDPS"
     gene            1947..2120
                     /locus_tag="PLQ18_09045"
     CDS             1947..2120
                     /locus_tag="PLQ18_09045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE01839.1"
                     /translation="MQRLIHRQRLRRIQDQQQQHALRRGRGQVWELAQMLDNADGKRD
                     SDRPAAEPVRQPH"
     gene            complement(2175..>2826)
                     /gene="lnt"
                     /locus_tag="PLQ18_09050"
     CDS             complement(2175..>2826)
                     /gene="lnt"
                     /locus_tag="PLQ18_09050"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HNE01840.1"
                     /translation="AELRAWAAETGTDLLIGAPDGDRARRDYRNAVASIGRTPGVYAK
                     HRLLPFGEYLPLRGLLMIFRDFVQIPMADFTAGAADQPLLVAAGVPVGVSICFESVFG
                     SEVRRALPAAQLLVVVSNDAWFGDSLAPHQHLQIAQMRALEAARPLVRATNTGLTAIV
                     DERGRITAQAPAFVETVLDGDVQPRSGVTPYVRFGDGPAAGLAAVLLLAGALRRRG"
BASE COUNT          434 a         1024 c          978 g          390 t
ORIGIN      
        1 gaagtcgagg ccgtcgaact cgccggaatg gatcagcgtg ccgtgctcgg cgtaggccgg
       61 ctgccagggc agcggcgtcg catcgccggc cggcgtgcgg atcaccggct cgaccggcag
      121 gccgtagcgg tgcgcgaact cgaagtcgcg ttcgtcgtgc gccggcacgg ccatcaccgc
      181 gccttcgccg taacccatca gcacgtagtt ggcgacccag accggcagct cgcggccggt
      241 gaacggatgg cgcacgcaca ggccggtcgg caggccgcgc ttttcctgcg tggcgatgtc
      301 ggcctcggcg acgccgccct tgcggcattc gtcgatgaac gcggcgagct ccggattgcc
      361 ggccgcggct tcgagcgcga gcgggtgctc ggcggcgacc gcgacgtagg tcgcgcccat
      421 cagcgtgtcg ggccgcgtcg tgtagacctt cacgccgccg tcggcaccga cgcgcgggaa
      481 cacgacatcc atgccgcgcg acttgccgat ccagttgcgc tgcatggtct tgacctgctc
      541 gggccagccc ggcagatcgt cgagcgcggc gagcagttca tcggcgtact gcgtgatccg
      601 gaagtagtac atcgggatct cgcgccgctc gaccagcgcg cccgaacgcc agccgcggcc
      661 gtcgatgacc tgctcgttgg cgagcacggt ctggtcgacc ggatcccagt tgacgacgcc
      721 gttcctgcgg tagatgacgc ccttctcgaa caggcgcgtg aacagccact gctcccagcg
      781 gtagtaatcg ggcgtgcagg tggcgagttc gcgcgaccag tcgatgccga gaccgagcga
      841 cttcagctgc gcgcgcatgt agtcgatgtt ctcgtaggtc cagcgcgccg gcgcgacgct
      901 gttcttgatc gcggcgttct cggccggcat gccgaaggcg tcccagccca tcggctgcac
      961 gacgttccgg ccgagcatgc gctggtagcg cgcgatcacg tcgccgatcg tgtaattgcg
     1021 cacatggccc atgtgcagcc ggccgctcgg gtacgggaac atcgacaggc agtagaactt
     1081 ctcgcgcgcc gggtcggcgg cgacctcgaa gctgcgctgc tcgttccagt agcgctgcgc
     1141 ggcggcctcg acggcctgcg gctggtagga ctcttcgatc atgctcggcc cggggctcag
     1201 gtggcgttcc gaaaaaggga cgaagcatac ccgaggccgc gccgtacggc acagtgcagc
     1261 acggaccgtg cttgccggcc gtgagcgccg ggtcgagact gcaggccggg aacgccgtac
     1321 ggaggaaacc ccgccatgac cgaaccgcgc caccgcctcg ccgaggccta cgcccggctg
     1381 ctcgaacgcc tgcacctgca cgacgccgac cgggacgcct cggaagccac gccgccgctg
     1441 cgcgcgcgca tcgaccgcgc cgccgcggac gcccatcacc tcggcgaact gacgaaggag
     1501 gaagccggcc tgctcgcgca ctgggcgcgg cgcgacctcg ccgacgccgg ccgccatctc
     1561 gccgagaccg gccaggagct cggcagctgg ctgcgcttcg acaccgaact ggtcgaggaa
     1621 cgcctgctcg actggttcgg gcgcgcggcc gaccagtcac ggctggagct gctggaattc
     1681 gcggacacgc tggaacggct cagtcattac gagaccggcg aggtcaccgg ccccggcacg
     1741 ctggcctgcg aggcctgcgg cgagcgcatc gagttccgcg gcaccggcac gattccgtcg
     1801 tgcccgaaat gcgggcatac gcgcttcgaa cgccccgtcg atccgtcgta agacggaagt
     1861 tcccggctta aaaaaatttc attttcagga acacgcgcgt atgctcgcgc tcgcccgcct
     1921 cggggcccgt taccggtacg accgccatgc agcgactcat ccaccggcag cgcctgcgcc
     1981 gtatccagga ccagcagcag cagcacgcgc tgcgtcgtgg ccgcggccag gtctgggaac
     2041 tcgcccagat gctcgacaac gccgacggca agcgcgattc cgatcgcccc gccgccgagc
     2101 cggtccggca accgcactga cgccatgacg aaccggcggt ccggcaggga agccggccgc
     2161 aaccgccggt ctcgtcagcc ccgccggcgc agggctccgg ccagcagcag cacggccgcc
     2221 agccctgccg ccggcccgtc cccgaagcgc acgtagggcg tgacgccgct gcgcggctgc
     2281 acgtcgccat cgagcacggt ctcgacgaag gccggcgcct gcgccgtgat ccgcccgcgt
     2341 tcatcgacga tcgccgtgag gccggtgttg gtcgcgcgca ccagcggccg cgcggcctcc
     2401 agcgcccgca tctgcgcgat ctgcaggtgc tggtgcggcg ccaggctgtc gccgaaccag
     2461 gcgtcgttgc tgaccaccac cagcagctgc gccgccggca gcgcgcggcg cacctcgctg
     2521 ccgaacaccg actcgaaaca gatcgacacg ccgaccggca cgccggccgc gaccagcagc
     2581 ggctggtcgg cggctccggc cgtgaagtcg gccatcggga tctgcacgaa gtcgcggaag
     2641 atcatcagca gcccgcgcag cggcaggtat tcgccgaagg gcagcagccg gtgcttggcg
     2701 tagacgccgg gcgtgcgccc gatcgacgcg acagcgttgc ggtagtcgcg ccgggcgcga
     2761 tcaccgtccg gcgcgccgat caggaggtcg gtgccggttt cggccgccca ggcgcgcagt
     2821 tctgcg
//
LOCUS       DAOQJH010000382         2815 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_24629_length_2815_cov_3.707609, whole genome shotgun sequence.
ACCESSION   DAOQJH010000382 DAOQJH010000000
VERSION     DAOQJH010000382.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2815)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2815)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2815
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24629_length_2815_cov_3.707609"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..365)
                     /locus_tag="PLQ18_09055"
     CDS             complement(<1..365)
                     /locus_tag="PLQ18_09055"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNE01841.1"
                     /translation="MLARLEIADFRNLAAVRLDCAPGLNLISGDNAAGKTSLLEAVYL
                     LGRARSFRTQRPAELIRRGAAHWRLVARLGADGSAGTLGLQRDGAQFTARLNGAPLPS
                     LAELARVLPVLLLTPDSHRL"
     gene            complement(385..1482)
                     /gene="dnaN"
                     /locus_tag="PLQ18_09060"
     CDS             complement(385..1482)
                     /gene="dnaN"
                     /locus_tag="PLQ18_09060"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008283669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HNE01842.1"
                     /translation="MKLVTPRESLLKPLAAVIGVVERRQTLPVLANVLMQARDGQLTL
                     TATDLEVELVAQAGLPADDGEITLPARKLHDILKSLPDEAEVTLAVDGERALLKSGRS
                     RFQLATLPAHDFPNTEDIPFEAELPIAQGALKRLIERTQFAMAQQDVRFYLNGLLLDW
                     TGSELRAVATDGHRLALCTLAVEGVSAAPLQVILPRKGVQELGRLLGDGETPAVLRIG
                     TNHAQVVLGDIRFTTKLIDGKFPDYERVLPRVGSRAILADRLVLRTALTRAAILSNEK
                     YRGVRLQAGDWLLKVQAHNPEQEEAEEEVEVNYSGEAFEIGFNVAYLLDALGALPGEL
                     VKLAFTDSSSSCLIQDPESDSARYVIMPMRL"
     gene            complement(1713..>2815)
                     /gene="dnaA"
                     /locus_tag="PLQ18_09065"
     CDS             complement(1713..>2815)
                     /gene="dnaA"
                     /locus_tag="PLQ18_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013981173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HNE01843.1"
                     /translation="RPRPAPALAPMPFAAAPEPPPPAAEQAQSNLNPNFTFDSFVAGK
                     SNQLARAACQQVAQNPGKAYNPLFIYGGTGLGKTHLMHAVGNQLRRQNPSARIVYLHS
                     EHFVADMVRALQHDRIDEFKRFYRSLDALLIDDIQFFAGKERSQEEFFHTFNALLEGN
                     QQVILTCDRYFKEVNGLEDRLKSRCGWGLTVVVEPPELETRVAILMSKAVQAQIVLPH
                     EAAFFIAKHIRSNVRELEGALLRVNANAQFTGRPVTLELCREALHDVISLHDKLVTID
                     NIQKTVAEYYKLRVADLNSPSRNRSIARPRQLAMALAKELTNHSLPEIGNAFGGRDHT
                     TVLHAVRKIAELREADTRIREDFNNLLATLST"
BASE COUNT          402 a          904 c         1027 g          482 t
ORIGIN      
        1 aggcgatggc tgtccggcgt cagcagcagc accggcagca cgcgcgccag ctcggcgagc
       61 gagggcagcg gcgcgccgtt gagccgggcc gtgaactgcg cgccgtcgcg ctgcaggccg
      121 agcgtgccgg cgctgccgtc ggcgccgagg cgcgcgacca gccgccagtg cgcggccccg
      181 cgccggatca gctcggccgg acgctgcgtg cggaacgagc gcgcgcgtcc gagcaggtag
      241 acggcttcga gcaggctggt cttgccggca gcgttgtcgc cgctgatcag gttcaggccg
      301 ggggcgcagt cgaggcgcac ggccgcgaga ttgcgaaagt cggcgatctc caggcgcgcg
      361 agcatgttcc ggcgtggccg gcggtcagag ccgcatcggc atgatcacgt agcgcgcgct
      421 gtcgctctcg ggatcctgga tcaggcagct cgaactggaa tcggtgaacg cgagcttgac
      481 cagctcgccg ggcagcgcgc cgagcgcatc gagcaggtag gcgacgttga agccgatctc
      541 gaaggcctcg ccgctgtagt tgacctcgac ttcctcctcg gcctcctcct gctcggggtt
      601 gtgcgcctgc accttcaaca gccagtcgcc ggcctgcagg cgcacgccgc ggtacttctc
      661 gttcgacagg atcgcggcgc gggtcagcgc cgtgcgcagc accagccggt cggcgaggat
      721 cgcgcgcgag ccgacgcgcg gcagcacgcg ctcgtagtcc gggaacttgc cgtcgatcag
      781 cttcgtcgtg aagcggatgt cgccgagcac gacctgcgcg tggttggtgc cgatgcgcag
      841 cacggccggc gtctcgccgt cgccgagcag ccggccgagt tcctgcacgc ccttgcgcgg
      901 caggatcacc tgcagcggcg cggcgctcac gccttcgacc gccagcgtgc agagcgccag
      961 ccggtgaccg tcggtggcga ccgcgcgcag ttcgctgccg gtccagtcga gcagcaggcc
     1021 gttcaggtag aagcgcacgt cctgctgcgc catcgcgaac tgcgtgcgct cgatcagccg
     1081 cttcagcgcg ccctgcgcga tcggcagctc ggcctcgaag gggatgtcct cggtattcgg
     1141 gaagtcgtgc gccggcagtg tcgcgagctg gaagcggctg cggccggact tcagcagtgc
     1201 gcgctcgccg tcgacggcga gcgtcacctc ggcctcgtcc ggcaggctct tcaggatgtc
     1261 gtgcagcttg cgcgccggca gcgtgatctc gccgtcgtcg gccggcagtc cggcctgcgc
     1321 gaccagttcc acttccagat ccgtggcggt cagcgtcagc tggccgtcac gggcctgcat
     1381 cagcacgttc gcgagcacgg gcagtgtctg gcggcgttcg acgacaccga tcacggcggc
     1441 gagcggcttc agcagcgatt cacggggcgt cacgagcttc atcggcgaac ctcttctgat
     1501 cctgtgtcct ttatataaaa gacttgttgt tgctgtgtgg cgtccgcgct gtggatgaat
     1561 ccggaaagcc cttgtcgatc agggcgatga aggaaactat cggtgcaaaa ccggtggacg
     1621 atagttgcgc tcgatcgcgg gtttcctgtg gatgaaaccg gggtttgtcc acaggccggg
     1681 ttttcccgga tccatccaca gcttgttcag gctcatgtgg acagggttgc caacaggtta
     1741 ttgaagtcct cgcggatgcg ggtgtcggcc tcgcgcagct cggcgatctt gcgcaccgcg
     1801 tgcaggacgg tcgtgtggtc gcgcccgccg aaggcgttgc cgatctcggg caggctgtgg
     1861 ttggtcagtt ccttggccag ggccatcgcc agctggcgcg ggcgggcgat cgagcggttg
     1921 cgcgagggcg agttcaggtc ggcgacgcgc agcttgtagt actcggcgac cgtcttctgg
     1981 atgttgtcga tcgtgaccag cttgtcgtgc agcgagatca cgtcgtgcag cgcctcgcgg
     2041 cagagctcca gcgtcaccgg ccggccggtg aactgcgcgt tggcgttgac gcgcagcagc
     2101 gcgccttcga gctcgcggac gttggagcgg atgtgcttgg cgatgaagaa cgccgcctcg
     2161 tgcggcagca cgatctgcgc ctgcacggcc ttgctcatca ggatcgcgac gcgcgtctcc
     2221 agctccggcg gctcgacgac cacggtcagg ccccagccgc agcgcgactt caggcggtcc
     2281 tccagaccgt tgacctcctt gaagtagcgg tcgcaggtca ggatcacctg ctggttgcct
     2341 tcgagcagcg cgttgaaggt gtggaagaac tcctcctgcg agcgctcctt gccggcgaag
     2401 aactggatgt cgtcgatcag cagcgcgtcg agcgagcggt agaagcgctt gaactcgtcg
     2461 atgcggtcgt gctgcagcgc acgcaccatg tcggcgacga agtgctcgga gtgcaggtag
     2521 acgatgcgcg ccgacgggtt ctggcggcgc agctggttgc cgaccgcgtg catcaggtgc
     2581 gtcttgccca gcccggtgcc accgtagatg aacagcgggt tgtaggcctt gccggggttc
     2641 tgggcgacct gctggcaggc ggcgcgcgcg agctggttcg acttgccggc gacgaagctg
     2701 tcgaaggtga agttcgggtt caggttgctc tgcgcctgct cggcagccgg cggcggcggc
     2761 tcgggcgcgg cggcgaacgg catcggcgcg agcgcgggag ccgggcgcgg gcggg
//
LOCUS       DAOQJH010000383         2813 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_24664_length_2813_cov_3.510152, whole genome shotgun sequence.
ACCESSION   DAOQJH010000383 DAOQJH010000000
VERSION     DAOQJH010000383.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2813)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2813)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2813
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24664_length_2813_cov_3.510152"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..433
                     /locus_tag="PLQ18_09070"
     CDS             <1..433
                     /locus_tag="PLQ18_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011615325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HNE01844.1"
                     /translation="GVWAPHAATLVIGVTFLLLLALVGPWTYTDLLAELAHGPAMAAG
                     PRLLLFAALLAGAAWGGLSAGRFRPLAPRLSALVRCTAGGALMGLGSLLIPGGNDGLL
                     LLGMPLLWPHAWLAFAVMAVTIALWLTLAARVPLRFSRRPA"
     gene            complement(414..1331)
                     /locus_tag="PLQ18_09075"
     CDS             complement(414..1331)
                     /locus_tag="PLQ18_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010166580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNE01845.1"
                     /translation="MDRLVAMAMFVRVVESGSFSAVARELDTTQPNVSKRVRALEARL
                     GSRLIARSTRQLSLTDEGRRYYEDCRRILDAVDAAEHSFLTGREVVAGPLRVAAPVSF
                     GRRRIAARLGDFLDRFPQVTVELLLSDDNSDLVADNIDVALRLGPLADSGLIARSLGQ
                     ARRITAATPGYLARRGEPRTPAELAGHDCLVFTPTPTVNLWHYRHGGERLSVSVGGRV
                     RTNSSEAIRELALAGLGIARAPRWLFGDELADGRIVPILRDYEPEPIPIHAVSPANRR
                     QSARVHAFIDYLASIWAADPQAGITPAGG"
     gene            1504..1770
                     /locus_tag="PLQ18_09080"
     CDS             1504..1770
                     /locus_tag="PLQ18_09080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE01846.1"
                     /translation="MNARTLITAAALTFAVAGFAQARESDSYPPIEWTHGDAAQTTPA
                     TAPRTAATDSFDARMRATGSDEHYCALDYPLNPKTGTNAEASLC"
     gene            2168..2467
                     /locus_tag="PLQ18_09085"
     CDS             2168..2467
                     /locus_tag="PLQ18_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HNE01847.1"
                     /translation="MSSNLHTTATDPEHLALQAELADIIASAVNLNRPATSIDPDEAL
                     YGSPGLGLDSIDILEVALAVSQRYGFSLRADSEDNLRIFSSLAHLAAHVAAHRSC"
     gene            2487..>2813
                     /locus_tag="PLQ18_09090"
     CDS             2487..>2813
                     /locus_tag="PLQ18_09090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE01848.1"
                     /translation="MSSNSVAPPATPGMARPWLKPLATAVAGAAYLGLIHVTTAPGEP
                     SDLGAAIAIVPPLVLSGCWAGRSLQAQPLRSAGWLVLCVLVAVLWPWLRDGFAWLYLL
                     QHAGAFA"
BASE COUNT          399 a         1054 c          919 g          441 t
ORIGIN      
        1 cggcgtctgg gcgccgcacg cggcgacgct ggtcatcggc gtgaccttcc tgctgctgct
       61 ggcgctggtc gggccgtgga cctacaccga tctgctcgcc gaactcgcgc acggtccggc
      121 gatggccgcc ggcccgcggc tgctgctgtt cgcggcgctg ctcgccggcg cagcctgggg
      181 cgggctgagc gccgggcgtt tccggccgct ggcaccgcgc ctgagcgcgc tggtccgctg
      241 cacggccggc ggtgcgctga tgggtctcgg cagcctgctg atccccggcg gcaacgacgg
      301 cctgctgctg ctcggcatgc cgctgctgtg gccgcacgcc tggctcgcgt tcgcggtcat
      361 ggccgtgacc atcgcgctgt ggctcacgct cgcagcgcgc gtgccgctgc gcttcagccg
      421 ccggccggcg tgatgccggc ctgcggatcg gccgcccaga tcgacgccag ataatcgatg
      481 aaggcatgca cgcgcgccga ctggcggcgg ttggccggcg acaccgcgtg gatcgggatc
      541 ggctccggct cgtagtcgcg caggatcggc acgatgcggc catcggccag ttcgtcgccg
      601 aacagccagc gcggcgcgcg cgcgatgccg agcccggcga gcgccagctc gcggatcgcc
      661 tcggagctgt tggtgcgcac gcgcccgccg acgctgaccg acagccgctc gccgccgtgg
      721 cggtagtgcc agaggttgac ggtcggcgtc ggcgtgaaca ccaggcagtc gtgcccggcg
      781 agttcggccg gcgtgcgcgg ctcgccgcga cgcgcgagat atcccggcgt cgcggccgtg
      841 atccggcgcg cctgcccgag cgagcgcgcg atcaggccgc tgtcggcgag cggcccgagg
      901 cgcagcgcca cgtcgatgtt gtcggcgacc agatcgctgt tgtcgtcgct gagcagcagc
      961 tcgaccgtca cctgcggaaa gcggtccagg aaatccccga gccgcgccgc gatccgccgc
     1021 cggccgaagc tgaccggcgc ggcgacacgc agcggcccgg cgacgacctc gcgcccggtc
     1081 aggaagctgt gctcggcggc atcgacggca tcgaggatgc gccggcagtc ctcgtaatag
     1141 cgccggccct cgtcggtcag cgacagctgg cgcgtgctgc gcgcgatcag ccggctgccg
     1201 agccgcgctt cgagcgcgcg cacccgcttg ctgacattcg gctgcgtggt gtcgagttcg
     1261 cgcgccaccg ccgagaagct gcccgattcg accacgcgca cgaacatcgc catcgccacc
     1321 agtctgtcca tcttcgctcc gataccccga tgctccgatt catgccgcca gggcatgccg
     1381 actatatcga ttgcgcgtct tatcgccggg gccggcgcgg ccgagactgc acgccatccc
     1441 gccgcactgc agcaaggccg cgacggggga tccgaccaga acctgcctcc cgaggtccac
     1501 gccatgaacg ccagaaccct gatcaccgct gccgccctga ccttcgccgt cgccggtttc
     1561 gcccaggccc gcgagagcga cagctacccg ccgatcgaat ggacgcacgg cgacgccgcg
     1621 cagaccaccc cggccaccgc gccgcgcacc gccgccaccg acagcttcga tgcccgcatg
     1681 cgcgccaccg gcagcgacga gcactactgc gcactcgatt acccgctgaa cccgaagacc
     1741 ggcaccaacg ccgaagcgag cctgtgctga tgccgtcgca ggcatccggt ctgatcagaa
     1801 acctgatcag aaaccccgct tcggcggggt ttctgctttt caggaagtcc gaacggcttt
     1861 caccgcagct ttatccggcc cgcccggcgc gaccgggttg caggcacagg ggcgattccg
     1921 ggcttcagac ggctgcattg ccggacgtca gccctgcttg catcatggcg actgtcgcgt
     1981 tgcagcatcg aacgagcgga tggcttgagc atcccgtgca caacgataaa atgctgaagt
     2041 tccagcatga acaaaatgta accgtaccgc ctgcaacctg ctgccatcac cggcctgcct
     2101 ggtcgcaggc ctgcggtgcc gccagtgcgg actgtcaaga ctccgcatcc agtcagggat
     2161 cgcctccatg tcgtcgaatc tgcataccac cgccactgac cccgaacatc tcgccttgca
     2221 ggccgaactg gcggacatca tcgcctctgc cgtcaatctg aaccggcctg ccaccagcat
     2281 cgaccctgac gaagccttgt acggcagtcc cggactcggt ctcgattcga tcgacatcct
     2341 cgaagtcgcc ctggccgtct cgcagcgcta cggcttttcg ctgcgcgccg acagcgagga
     2401 caatctccgc atcttctcct ccctcgcgca tctcgccgcc catgtcgcgg ctcaccgcag
     2461 ctgctaggtc cggcggcggt tccccgatga gttccaattc cgttgcgccg ccggccaccc
     2521 ccggcatggc gcgcccctgg ctgaaaccgc tggccaccgc cgtggccggt gctgcttacc
     2581 tcggcctgat ccacgtcacg accgcccctg gcgaaccgag cgacctcgga gccgcgatcg
     2641 ccatcgtgcc gccgctggtg ctgtcgggat gctgggccgg acgcagcctg caggcacagc
     2701 cgctgcgttc ggccggctgg ctggttctct gtgtcctggt cgcggtgctc tggccgtggc
     2761 tgcgcgacgg cttcgcctgg ctgtacctgc tgcagcacgc cggcgccttc gca
//
LOCUS       DAOQJH010000384         2810 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_24718_length_2810_cov_5.286751, whole genome shotgun sequence.
ACCESSION   DAOQJH010000384 DAOQJH010000000
VERSION     DAOQJH010000384.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2810
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24718_length_2810_cov_5.286751"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..800
                     /locus_tag="PLQ18_09095"
     CDS             <1..800
                     /locus_tag="PLQ18_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018319501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HNE01849.1"
                     /translation="FGPRLAGAGGFINISQNAKRVVFVGTFTAGGLRLAIGNGQLHIE
                     QEGRERKFVRAVEHRTYSGPVAAARGQKALYVTERCVFELSAGGLVLTEVAPGIDIER
                     DILAHMDFAPLIPQPPRLMDARIFLPATMGLRAGLLELPLEQRFTYDAAQNLFFIDFG
                     GYHVARPEQIEAIRGVVETRLGHLDKRVDTIVNYDDFSIAPELLDAYIEMVRDVVGRF
                     YSGVTRYTTNAFLRMKLGRALSERELTPALYGSRDEARAGLASGSQR"
     gene            882..1745
                     /gene="motA"
                     /locus_tag="PLQ18_09100"
     CDS             882..1745
                     /gene="motA"
                     /locus_tag="PLQ18_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008329974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HNE01850.1"
                     /translation="MFVIIGYVVIIGCILGGFIMAGGHLGVLMQPVEVLIIVGSAVGA
                     FFVGNSMTVVKEAGGALGACFKGGKYTQALYMETLTLLYLIFAKIRKEGLLALQADID
                     EPHSSKLFEQAPTVLHDHHAVEFITDYLRLLSSGNIELNQLDNLMDLDIETHHHQGER
                     PIKAIQAFADGLPAFGIVAAVLGVVHTMESVGIPPAELGKLIAAALVGTFLGILLAYG
                     FVGPLGSLLTQKLDESTKYYQAIKAAFIANLNGYDPMASVEFARKVMYSSERPGFTEL
                     EKHIQAARKAK"
     gene            1796..>2810
                     /gene="motB"
                     /locus_tag="PLQ18_09105"
     CDS             1796..>2810
                     /gene="motB"
                     /locus_tag="PLQ18_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018059340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HNE01851.1"
                     /translation="MSEQRPIIIKKVKKGHGGHHGGAWKLAYADFVTAMMAFFLLMWL
                     LGSTTKGSLQGIAEYFQNPFKVAMSGGEGSGDANSVIRGGGEDLSRSVGQVKRTNEGT
                     RQILTKASEDQAGKLRLLKDKLERMIAASATLKQFRDQLKLDLTREGLRIQIIDAANR
                     PMFELGSAVMQPYARDILREIAPVIGELPNVLTITGHTDARPLGNPAKGYSNWELSAD
                     RANAARRELVGAGLSEQKILRVIGLAESVPFVPQDPLDPTNRRIAIVVMNKDAEDSVR
                     ANIGESVEVREGAGVRPETIDPDRAPPVPARPQGRPALIPPPAVAPAPATNGSAPPAS
                     PAPA"
BASE COUNT          483 a          987 c          918 g          422 t
ORIGIN      
        1 agttcgggcc gcgcctggcc ggcgccggcg gcttcatcaa catcagccag aacgccaagc
       61 gcgtggtgtt cgtcggcacc ttcacggccg gcggcctcag gctcgcgatc gggaacgggc
      121 agctgcacat cgagcaggaa ggccgcgagc gcaagttcgt gcgcgccgtc gagcaccgca
      181 cctacagcgg tccggtcgcc gccgcgcgcg ggcagaaggc gctgtacgtg accgagcgct
      241 gcgtgttcga gctgagcgcg ggcgggctgg tgctgaccga ggtcgccccc ggcatcgaca
      301 tcgagcgcga catcctcgcg cacatggact tcgcgccgct gatcccgcag ccgccgcgac
      361 tgatggatgc gcgcatcttc ctgccggcga cgatgggcct gcgcgccggg ctgctggagc
      421 tgccgctgga acaacgcttc acgtacgatg ccgcgcagaa tctgttcttc atagatttcg
      481 gcggctacca tgtcgcccgc cccgaacaga tcgaagcgat ccgcggggtg gtcgagacgc
      541 ggctgggaca cctcgacaaa cgcgtcgaca ccatcgtcaa ctacgacgac ttcagcatcg
      601 ccccggaact gcttgacgcc tatatcgaaa tggtcaggga cgtggtcggg cgcttctatt
      661 cgggcgtcac ccgttacacg accaatgcct tcctgcgcat gaaactcggc cgcgcgctct
      721 ccgagcgcga gctgacgccg gcgctgtacg gcagtcgaga cgaggcgcgg gcaggcctgg
      781 catccggcag ccagcgctga cggcggcccg gaccgggtgc ggcggcgcga cgcgcccggt
      841 cgtccgacaa gcccggcccg cggggggccg caaggtcgcg catgttcgtc atcatcggct
      901 acgtggtcat catcggctgc atcctcggcg gtttcatcat ggccggcggc catctcggcg
      961 tgctgatgca gccggtggaa gtgctgatca tcgtcggctc ggcggtcggc gctttcttcg
     1021 tcggcaacag catgacggtc gtcaaggagg cgggcggggc gctcggtgcc tgcttcaagg
     1081 gcggcaagta cacgcaggcg ctgtacatgg agacgctgac gctgctctat ctgatctttg
     1141 cgaagatccg caaggaaggc ctgctcgcgc tgcaggccga catcgacgag ccgcacagca
     1201 gcaagctgtt cgagcaggcg ccgaccgtgc tgcacgatca ccacgccgtc gagttcatca
     1261 ccgattacct gcgcctgctg tcctcgggca acatcgagct gaaccagctc gacaacctga
     1321 tggacctcga catcgagacc caccaccatc agggcgagcg accgatcaag gccatccagg
     1381 ccttcgccga cggcctgccg gccttcggca tcgtcgcggc cgtgctcggt gtcgtgcaca
     1441 cgatggagtc ggtcggcatc ccgccggccg agctcggcaa gctgatcgcc gcggcgctgg
     1501 tcggcacctt cctcggcatc ctgctcgcct acggcttcgt cggcccgctc ggcagcctgc
     1561 tgacgcagaa gctcgacgag agcaccaagt actaccaggc gatcaaggcg gcgttcatcg
     1621 ccaacctgaa cggctacgac ccgatggctt cggtcgaatt cgcgcgcaag gtcatgtact
     1681 caagcgagcg cccgggcttc accgagctgg aaaagcacat ccaggccgcc cgcaaggcga
     1741 agtgaacgcc tgatcctgcc cggtcctccg ggtctgcccg cagcggttgc gcctgatgtc
     1801 cgaacagcgt ccgatcatca tcaagaaggt caagaagggc cacggcggtc accacggtgg
     1861 cgcgtggaag ctcgcctatg cggacttcgt gaccgcgatg atggcgttct tcctgctcat
     1921 gtggctgctc ggctcgacca ccaagggctc gctgcagggc atcgccgagt acttccagaa
     1981 ccccttcaag gtcgcgatga gcggcggcga gggctcgggc gatgccaaca gcgtcatccg
     2041 cggcggcggc gaggatctca gccgctcggt cggtcaggtc aagcgcacca acgaaggcac
     2101 gcgccagatc ctcaccaagg cttccgagga tcaggccggc aagctccggc tgctgaagga
     2161 caagctggag cgcatgatcg cggccagcgc gacgctcaag cagttccgcg accagctcaa
     2221 gctcgacctg acccgcgagg gcctgcgcat ccagatcatc gatgccgcca accggccgat
     2281 gttcgaactc ggcagcgcgg tcatgcagcc ctatgcgcgc gacatcctgc gcgagatcgc
     2341 accggtcatc ggcgaactgc cgaacgtgct gaccatcacc ggccacaccg atgcccgccc
     2401 gctcggcaac ccggccaagg gctactcgaa ctgggagctg tcggccgatc gcgccaacgc
     2461 cgcccgccgc gaactggtcg gtgccggcct cagtgaacag aagatcctgc gcgtgatcgg
     2521 gctggccgag agcgtgccgt tcgtgccgca ggacccgctc gatccgacca accgccgcat
     2581 cgcgatcgtg gtcatgaaca aggatgccga ggacagcgtg cgcgccaaca tcggcgaatc
     2641 ggtcgaggtc cgcgaaggtg caggcgtgcg gcccgaaacc atcgatccgg atcgcgcgcc
     2701 gccggtgccg gcacggcccc agggccggcc ggccctgatc ccgcctccgg ccgtcgcccc
     2761 cgcgccggcc accaacggca gcgcgccgcc cgcgtcacct gcgcccgcca
//
LOCUS       DAOQJH010000385         2796 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_24931_length_2796_cov_3.224735, whole genome shotgun sequence.
ACCESSION   DAOQJH010000385 DAOQJH010000000
VERSION     DAOQJH010000385.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2796)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2796)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2796
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24931_length_2796_cov_3.224735"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..532
                     /locus_tag="PLQ18_09110"
     CDS             <1..532
                     /locus_tag="PLQ18_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012849712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNE01852.1"
                     /translation="GHLVPGMQGRLQAVEGLEPGTGLLEVRGPNVMLGYLRHERPGLI
                     EPPAAAGETGWYSTGDIVSIDEDGYVFIRGRLKRFAKLAGEMVSLEVVEQIARTAAPE
                     ARHAAATRADPARGEAIVLFTTDAALGREALLAAARTLGAPELAVPRDIRALAEIPLL
                     GTGKTDYVRLQTLAAS"
     gene            622..1056
                     /locus_tag="PLQ18_09115"
     CDS             622..1056
                     /locus_tag="PLQ18_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNE01853.1"
                     /translation="MPNRPVREVIANQTILTAPAAISVSEAAVRMKESRVGAVMVVET
                     DGALSGIFTERDALFRVLAAGLDPKTTKLAEVMTPHPQTIAPERPFGHALHMMYECGF
                     RHVPVIDAGKPIGMVSARDALGPELAVFQTEVQQREHIEEIL"
     gene            1153..1449
                     /locus_tag="PLQ18_09120"
     CDS             1153..1449
                     /locus_tag="PLQ18_09120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE01854.1"
                     /translation="MRPSARAAVLAALLAGLPASLPADPLAIDAGDDVGTLLNKYQGK
                     RVTLRLVSGEELTGTVRLVSARLVHISELASREFYDAAVVTGSIAAVIVRTRTQ"
     gene            1563..2774
                     /gene="lplT"
                     /locus_tag="PLQ18_09125"
     CDS             1563..2774
                     /gene="lplT"
                     /locus_tag="PLQ18_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016538055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HNE01855.1"
                     /translation="MTPFPSAAAAPGPLWSRGMAAVLFAQFFSAFGDNAVLFSALALL
                     RAEAYPDWAAPLLQQFFVAAYILLAPFAGPFADSLPKGRVMLAGNGLKLAGAAGLTLG
                     LNPFLAYGLIGVGAAAYSPAKYGILGELTTGDNLVKANGLMEASTIAAILAGAIAGGV
                     LADWSVGGALLVCTGVYALAAAANLLIPRLPPAHPIADARPGALLADFLAACRTIWQH
                     PDTRFSILGTSLFWGSGVTMRFLLVAWVPIALGLHDNATPAYLNAMVALGIVLGAGLA
                     GALVRLEQVERALPAGVGIGLAVALLALVNALVPAYALLAVVGACGGFFIVPLNALLQ
                     ERGRETVGAGHAIAIQNLGENGAMLAMLGLYTVALGAGTPVLWIAPLFGLGLSAAIAA
                     LWISRRRARAH"
BASE COUNT          368 a         1034 c          969 g          425 t
ORIGIN      
        1 cgggcatctg gtgccgggca tgcaggggcg gctgcaggcg gtcgagggcc tggagccggg
       61 caccggcctg ctcgaagtgc gcgggccgaa cgtcatgctc ggctacctgc gccacgaacg
      121 cccgggcctg atcgagccgc cggcggcggc cggggaaacg ggctggtatt cgaccggcga
      181 catcgtctcg atcgacgagg atggctacgt gttcatccgc ggacggctga agcgcttcgc
      241 caagctcgcc ggcgagatgg tgtcgctcga agtcgtcgag cagatcgcgc gcacggccgc
      301 gcccgaagcc cggcacgcgg cggccacgcg cgccgatcct gcgcgcggcg aggccatcgt
      361 gctgttcacg accgatgccg cgctcgggcg cgaggcgctg ctcgcggcgg cgcgcacgct
      421 cggtgccccg gaactggccg tgccgcgcga catccgtgcg ctggcggaga tacccttgct
      481 cggcaccggc aagacggact acgtccggct gcaaacgcta gccgcatcgt gaaaacgttt
      541 gtagcttcag cagaccacga ctgtgctgca catcgactag aaaccactgg ttgagacaaa
      601 ccccgccgag aggtgcctgc catgcccaac cgccccgtac gcgaagtcat cgccaaccag
      661 acgatcctga cggctccggc cgccatcagc gtcagcgagg ccgccgtgag gatgaaggaa
      721 agccgcgtcg gcgccgtgat ggtcgtcgaa accgacggcg ccctcagcgg catcttcacc
      781 gagcgcgatg ccctgttccg cgtgctcgcc gccggccttg atccgaagac gacgaagctc
      841 gccgaggtca tgaccccgca tccgcagacg atcgcccccg aacgcccgtt cggccatgcc
      901 ctgcacatga tgtatgagtg cggcttccgc cacgtgccgg tcattgatgc cggcaagccg
      961 atcggcatgg tctcggcgcg cgatgcgctc ggccccgagc tggccgtgtt ccagaccgaa
     1021 gtgcagcagc gcgagcacat cgaggaaatc ctgtaagccc accgcgccag ccccgaccgg
     1081 gctgccgatc cggccgtgtg ccgtccgcga gcggtgcacg gccttttcat gtccgccgcg
     1141 aggagacccg ccatgcgtcc ttccgcccgc gccgccgtgc ttgccgcgct gctggccggc
     1201 ctgcctgcca gcctgcccgc cgatccgctc gccatcgacg ccggtgatga tgtcggcacg
     1261 ctgctgaaca agtaccaggg caagcgcgtg acgctgcgcc tggtttccgg tgaggaactg
     1321 accggcacgg tgcgtctggt cagtgcccgg ctcgtgcaca tcagcgaact cgccagccgc
     1381 gagttctacg acgcggcggt cgtgaccggc agcatcgccg cggtgatcgt gcgcacgcgc
     1441 acgcagtaag ttcgggcagg cagcaccggc gcgccggctg cctccgcccg cgcctcccgg
     1501 ccggccggcg accggcgccg gctacactgc cggcacccaa ccgccgcccg gatgcccccc
     1561 gaatgactcc gtttccgtct gcggccgccg cacccggccc gctgtggtcg cgcggcatgg
     1621 ccgccgtgct gttcgcgcag ttcttctcgg ccttcggtga caacgcggtg ctgttctcgg
     1681 cgctggcgct gctgcgcgcc gaggcctatc cggactgggc cgcgccgctg ctgcagcagt
     1741 tcttcgtcgc cgcctacatc ctgctcgcac ccttcgccgg gccgttcgcc gacagcctgc
     1801 ccaagggccg ggtgatgctg gccggcaacg gcctgaagct cgccggggcc gccgggctga
     1861 cgctggggct gaatcccttc ctcgcctacg ggctgatcgg cgtcggcgcc gcggcctatt
     1921 cgccggcgaa gtacggcatc ctcggcgagc tgaccaccgg cgacaatctg gtcaaggcca
     1981 acggcctgat ggaagcctcg accatcgccg caatcctcgc cggagccatc gccggcggcg
     2041 tgctcgccga ctggagcgtc ggcggtgcgc tgctggtctg caccggcgtc tacgcgctcg
     2101 ccgccgccgc caacctgctg atcccgcgcc tgccgccggc acacccgatc gccgatgccc
     2161 ggcccggcgc gctgctcgcg gatttcctcg ccgcctgccg cacgatctgg cagcaccccg
     2221 acacgcgctt ctcgatcctc ggcaccagcc tgttctgggg ctcgggcgtg acgatgcgct
     2281 tcctgctggt ggcctgggtg ccgatcgcgc tcggcctgca cgacaacgcc acgccggcct
     2341 atctgaacgc gatggtcgcg ctcggcatcg tgctcggggc ggggctcgcc ggcgcgctgg
     2401 tgcggctcga acaggtcgaa cgtgcgctgc cggccggcgt cggcatcggc ctggcggtgg
     2461 cgctgctcgc gctcgtgaac gcgctggtgc cggcctatgc gctgctcgcc gtggtcgggg
     2521 cctgcggcgg atttttcatc gtgccgctga acgcgctgct gcaggagcgc gggcgcgaga
     2581 cggtcggggc cggccacgcg atcgcgatcc agaacctcgg cgagaacggc gcgatgctgg
     2641 cgatgctcgg gctgtacacg gtcgcgctcg gggccggcac gccggtgctg tggatcgcgc
     2701 cgctgttcgg actcggcctg tcggcggcga tcgcggcgct ctggatcagc cgccggcgcg
     2761 cgcgcgcgca ctgagcccgg ggttcagcgg cgcgtc
//
LOCUS       DAOQJH010000386         2790 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_25046_length_2790_cov_3.021938, whole genome shotgun sequence.
ACCESSION   DAOQJH010000386 DAOQJH010000000
VERSION     DAOQJH010000386.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2790)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2790)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2790
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25046_length_2790_cov_3.021938"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..727)
                     /locus_tag="PLQ18_09130"
     CDS             complement(<1..727)
                     /locus_tag="PLQ18_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018904176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HNE01856.1"
                     /translation="MSDEVLKQLALQYHRAPTPGKIEVVPTKPLATQRDLALAYAPGV
                     TAACEAIARDPAEVANLTARGNLVAVVTNGSSVLSLGPLGPLAAKPVMEGKGVLFKKF
                     AGIDVFDIELDENDPSMLVEVIARMEPTFGGILLEDIKAPECFEVEQRLRERMKIPVF
                     DDDQHGTAIVVAAALLNGLRVVGKHISEIRLVCAGVGASAVACLDLLVRLGLPAARIS
                     VIDSEGVLHSGRAERLDPYKAKWV"
     gene            complement(849..1262)
                     /locus_tag="PLQ18_09135"
     CDS             complement(849..1262)
                     /locus_tag="PLQ18_09135"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008066480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HNE01857.1"
                     /translation="MSDPLPSLQPEGEPAIRTIAMPADANPAGDIFGGWLMAQMDLAA
                     GNVAARRARGRCVTVSVDHIAFLSPVYVGDEVSLHARVVRVGRTSMTIHVEAWRRSRD
                     GDDRVRVTEANFTFVALDQGRRPRVLPPPTAQDPC"
     gene            complement(1358..2536)
                     /locus_tag="PLQ18_09140"
     CDS             complement(1358..2536)
                     /locus_tag="PLQ18_09140"
                     /inference="COORDINATES: protein motif:HMM:NF025167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HNE01858.1"
                     /translation="MRIVDDAPPRAWRQFAWCSVRLLAVVLAMFAAGVVPVLAHPVAQ
                     GAMELQLEPGRIVLDVHASMEQVLVQAAYGPAATATLPERIRLHGDYLRAHLRLAADD
                     VALQAGPPEALAARPGRAAWRLVFPLPAGSRPARLRLWQDVLREIEYAPGNPWEASYV
                     LRVSGAGAGEGGLLASAQTLSLSLSPAPSAPAAAPPAPAVPVAQWIAHGLRHILAGYD
                     HLLFVAALTLGARRLGELVWLVGAFTLAHTLTLALSALDVIRLPSGLVEPMIAASIVA
                     VALLNVFGSTDARTPRRRLLCAFGFGLFHGLGFAGGLLEAFADLGGGAAVTAILAFSV
                     GVEGGHLAVVLPLWVLLAALRHRRAGSDPRALRRGASVAISLAGLLYLATALGHGPLA
                     "
     gene            complement(2523..>2790)
                     /locus_tag="PLQ18_09145"
     CDS             complement(2523..>2790)
                     /locus_tag="PLQ18_09145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE01859.1"
                     /translation="LPFAARDLQLRPNFLAADQYAWALYRAGRYDEAKAASEPVLAAG
                     AADAPLLVHAALIRLATGDAQQGRALLARAAAVNPRYEAFHAHR"
BASE COUNT          441 a         1009 c         1017 g          323 t
ORIGIN      
        1 gcacccactt cgccttgtac gggtcgagcc gctcggcgcg gccgctgtgc agcacgccct
       61 cggaatcgat gacgctgatg cgcgccgccg gcaggccgag ccgcaccagc aggtcgaggc
      121 aggcgaccgc cgaggcgccg acgccggcgc agacgaggcg gatctcggag atgtgcttgc
      181 cgaccacgcg caggccgttg agcagcgccg cggcgacgac gatggccgtg ccgtgctggt
      241 cgtcatcgaa caccgggatc ttcatgcgct cgcgcaggcg ctgctcgacc tcgaagcact
      301 ccggcgcctt gatgtcttcg agcaggatgc cgccgaaggt cggctccatg cgcgcgatga
      361 cctcgaccag catcgacggg tcgttctcgt cgagttcgat gtcgaacacg tcgatgccgg
      421 cgaacttctt gaacagcacg cccttgcctt ccatgaccgg cttcgcggcc agcggcccga
      481 gcggcccgag gctcagcacc gacgagccgt tggtgacgac ggcgaccagg ttgccgcgcg
      541 cggtgaggtt ggcgacctcg gccggatcgc gggcgatcgc ctcgcaggcg gcggtgacgc
      601 cgggcgcgta ggcgagcgcc aggtcgcgct gcgtggcgag cggcttggtc ggcacgacct
      661 cgatcttgcc gggcgtcggc gcccggtggt actgcagcgc aagctgcttc aggacttcgt
      721 cgctcatggc ggcgggacct ctgcgaatac ggcgactgcg atgaatgcgg cgaatacggc
      781 gaaaaatccg tcgaaactcg tcgaaatccg tcgggatgcg cccggaacag gacaggaacg
      841 tcagcggctc agcacgggtc ctgcgcggtc ggcggcggca gcacgcgcgg ccggcggccc
      901 tgatcgagcg cgacgaaggt gaagttggcc tcggtcacgc gcacgcggtc gtcgccgtcg
      961 cgcgagcggc gccaggcctc gacgtggatg gtcatcgacg tgcgcccgac gcgcacgacg
     1021 cgcgcgtgca ggctgacctc gtcgccgacg tagaccgggc tcaggaaggc gatgtgatcg
     1081 accgacaccg tcacgcagcg gccgcgggcg cggcgcgcgg cgacgttgcc ggcggcgaga
     1141 tccatctgcg ccatcagcca gccaccgaag atgtcgccgg ccggattggc atcggccggc
     1201 atcgcgatcg tgcggatcgc gggttcacct tcgggttgca gggaaggaag cggatcggac
     1261 atggacgacg gaaccggcag cggcggcggg gccgcacagc gtagcgcgag cctcggcaat
     1321 gctcatccgc gatgtgctgc accctgcgcg atggatttca cgccagcggg ccgtgcccga
     1381 gcgcggtggc caggtacagc agccccgcga gcgagatcgc gaccgaggcg ccccggcgca
     1441 gggcgcgcgg atcgctgccg gcgcgccggt ggcgcagcgc cgcgagcagc acccacagcg
     1501 gcagcacgac cgcgagatgc ccgccctcga cgccgacgct gaacgcgagg atggccgtga
     1561 cggccgcacc gccgccgaga tcggcgaagg cctccagcag cccgccggca aagccgaggc
     1621 cgtggaacag cccgaagccg aacgcacaca gcagccgccg gcgcggcgtg cgtgcatcgg
     1681 tgctgccgaa cacgttcagc agcgcaaccg cgacgatgct ggcggcaatc atcggctcga
     1741 cgaggccgga cggcagccgg atcacatcga gcgccgacag cgcgagcgtc agcgtgtgtg
     1801 cgagcgtgaa cgcaccgacc agccagacca gctcgccgag gcgccgcgca ccgagcgtca
     1861 gcgccgcgac gaacagcagg tggtcgtagc cggcgaggat gtgccgcagg ccgtgggcga
     1921 tccactgcgc caccggcacg gccggggccg gcggtgccgc ggccggagcg gacggcgccg
     1981 ggcttaacga caggctcagc gtctgcgccg aagccagcag cccgccttcg ccggctccgg
     2041 ccccggacac gcgcagcacg tagctcgcct cccacggatt gcccggcgcg tactcgatct
     2101 cgcgcagcac gtcctgccag agcctcaggc gcgccggccg gctgccggcc ggcagcggaa
     2161 acaccagccg ccacgccgcc cgccccggcc gcgccgccag ggcttcgggc ggaccggcct
     2221 gcagggccac gtcatcggcc gcgagcctca ggtgcgcacg cagatagtca ccgtgcaggc
     2281 ggatgcgctc gggcagcgtg gccgtggccg ccgggccgta ggcggcctgc accagcacct
     2341 gctccatcga cgcatgcacg tcgagcacga tgcggccggg ttcaagctgc agttccatcg
     2401 cgccctgcgc gaccggatgc gcaagcaccg gaacgacgcc ggcggcgaac atcgccagca
     2461 cgacggccag tagccgcacg ctgcaccacg caaactgccg ccacgcgcgc ggcggcgcgt
     2521 cgtcaacgat gcgcatggaa agcctcgtag cgcggattga ccgccgcggc ccgcgcgagc
     2581 agcgcccggc cctgctgtgc gtcgccggtc gcgagccgga tcagcgccgc gtgcacgagc
     2641 agcggcgcat cggcagcccc ggcggccagc accggctcgc tcgcggcctt cgcctcgtca
     2701 tagcgcccgg cgcggtacag cgcccacgcg tactgatcgg cggcgaggaa gttcggccga
     2761 agctgcaggt cgcgcgccgc gaacggcagc
//
LOCUS       DAOQJH010000387         2788 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_25077_length_2788_cov_3.865715, whole genome shotgun sequence.
ACCESSION   DAOQJH010000387 DAOQJH010000000
VERSION     DAOQJH010000387.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2788)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2788)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2788
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25077_length_2788_cov_3.865715"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(137..757)
                     /locus_tag="PLQ18_09150"
     CDS             complement(137..757)
                     /locus_tag="PLQ18_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009132331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HNE01860.1"
                     /translation="MTEPEPGKPRLLVVAGPNGSGKTTVTEQVLKHRWFDGCEYINPD
                     NIAEQQYGGWNSAEAVLQAAKHAQALRERCLAEGRDMAFETVFSADDKVQFIERALAA
                     GYFVRVFFVSTRHPSLNAARITYRVMNGGHDVSIAKIIARYRRSIVNCTLIARRVDRL
                     YVYDNSVDGAEARLMFRARYGELAKVYAGPIERWADSIHTALRAPA"
     gene            complement(750..968)
                     /locus_tag="PLQ18_09155"
     CDS             complement(750..968)
                     /locus_tag="PLQ18_09155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE01861.1"
                     /translation="MIQSAACRLDPLANDCEPTDEQLEMIMAAAARSAREIRARVDAE
                     LYRQIAVELEAAKARARARGSIFVQDHD"
     gene            complement(1064..2002)
                     /gene="msrP"
                     /locus_tag="PLQ18_09160"
     CDS             complement(1064..2002)
                     /gene="msrP"
                     /locus_tag="PLQ18_09160"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011385446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HNE01862.1"
                     /translation="MLIRRPSDLRPSEITSPELFHGRRRFLQGALAAGAAAAFGLPAV
                     ASAGEKLPNVVKSPYRVDEPLTPYDAVTEYNNFYELGTGKDDPQRNAARLKPKPWTVE
                     IAGACEQPGRVGFDDLIAGLALEERVYRHRCVEAWSMVVPWVGVPLGQVLAKYKPLSS
                     AKYVAFTTLMDPQQFPGQRLPVLEWPYREGLRMDEALHPLAILAVGVYGEVLPNQNGA
                     PLRLVVPWKYGFKGIKSIVKIEFVEHEPRTSWNLAAPDEYGFYANVNPAVDHPRWSQK
                     RERRIGDWLKRDTLAFNGYGEQVASLYSGMDLRKSF"
     gene            2219..2440
                     /locus_tag="PLQ18_09165"
     CDS             2219..2440
                     /locus_tag="PLQ18_09165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE01863.1"
                     /translation="MRENFSEDEESLDDDTEHESPAVVPTPPPAPSGPGSKAWRSIER
                     HRELRELRRHLDDFQFEDDPKYDPRNLPL"
     gene            2582..>2788
                     /locus_tag="PLQ18_09170"
     CDS             2582..>2788
                     /locus_tag="PLQ18_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010939017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HNE01864.1"
                     /translation="MIASFRDKDTCRLMEGEPVPRLAACRKAALRKLDMLAAAHVLQD
                     LRQPPGNRLEALQGNRAGQHSIRIN"
BASE COUNT          479 a          905 c          950 g          454 t
ORIGIN      
        1 aatcatggtc ggtcaggcgc agaacaagat cgacgccgac ggccagctca ccgacgccgg
       61 cacgcgcgaa ttcctcggca agcaactggc ggcgctggcg gcgctggcgc ggcagatggc
      121 gggctgaagg cgcggatcag gccggcgcgc gcagcgccgt gtggatgctg tcggcccagc
      181 gttcgatcgg gccggcatag accttcgcca gctcgccgta gcgggcccgg aacatcagcc
      241 gggcctcggc gccgtcgacc gagttgtcgt agacgtacag ccggtcgacg cggcgggcga
      301 tcagcgtgca gttgacgatc gagcgccggt agcgcgcgat gatcttggcg atcgatacat
      361 catggccgcc gttcatgacc cgataggtga tgcgggcggc gttgagggac gggtggcggg
      421 tcgagacgaa gaacacccgg acgaaatatc cggcggccag cgcccgctcg atgaactgca
      481 ccttgtcatc ggccgagaac accgtctcga aggccatgtc gcggccttcg gcgaggcagc
      541 gctcgcgcag cgcctgcgcg tgtttcgccg cctgcagcac ggcctcggcc gaattccagc
      601 cgccgtactg ctgctcggcg atgttgtcgg gattgatgta ttcgcagcca tcgaaccagc
      661 ggtgcttcaa cacctgctcg gtgaccgtgg tcttgccgga accgttcggt ccggcgacga
      721 ccagcaagcg tggcttgccg ggttcaggtt cagtcatggt cctgcacgaa gatgctgccg
      781 cgggcgcggg cgcgggcctt ggcggcttcc agctcgacgg cgatctgccg gtaaagctcg
      841 gcatcgacgc gcgcccgaat ctcgcgggcc gagcgggcag cggcggccat gatcatttcg
      901 agctgctcgt cggtcggctc gcagtcgttg gcgagcgggt cgaggcggca ggcggcggac
      961 tggatcattt cggtaacctc cggatggcag gtgcctgacg gaaaaagcct gttggcagtg
     1021 tatgcctgtc ggacaggcgg tcaatcgccg ccggcggccg gcctcagaac gacttgcgca
     1081 ggtccatgcc cgagtacagc gaggcgacct gctcgccgta gccgttgaat gccagcgtgt
     1141 cgcgcttcag ccagtcaccg atgcgccgct cgcgcttctg gctccagcgc gggtgatcga
     1201 cggccggatt gacgttggca tagaagccgt actcgtcggg cgcagcgagg ttccacgagg
     1261 tgcgcggctc gtgctcgacg aactcgatct tcacgatcga cttgatgccc ttgaagccgt
     1321 acttccacgg cacgaccagg cgcagcggcg cgccgttctg gttcggcagc acctcgccgt
     1381 agacgccgac ggcgaggatg gccagcggat gcagcgcctc gtccatgcgc agaccctcgc
     1441 gatacggcca ctccagcacc ggcaggcgct ggccgggaaa ctgctgcggg tccatcagcg
     1501 tcgtgaacgc cacgtacttc gcgctcgaca gcggcttgta cttcgcgagc acctgcccga
     1561 gcggcacgcc gacccagggc acgaccatcg accaggcctc gacgcagcgg tggcggtaga
     1621 cgcgctcctc cagcgccagt ccggcgatca gatcatcgaa accgacgcga ccgggctgct
     1681 cgcaggcgcc tgcaatctcg acggtccacg gcttcggctt caggcgtgcg gcgttgcgct
     1741 gcggatcgtc cttgccggtg ccaagctcgt agaagttgtt gtactcggtg acggcgtcgt
     1801 acggcgtcag cggctcgtcg acgcgatagg gacttttgac gacgttcggc agcttctcgc
     1861 cggcgctcgc gaccgccggc aggccaaacg cggcggccgc accggcagcg agcgccccct
     1921 gcaggaagcg gcggcggccg tggaacagtt cgggcgacgt gatctcggac gggcgcagat
     1981 cggacggacg gcggatcagc atgacggcga ctcccggatg tggacgaccg gactgcttac
     2041 gacgctgtgg cggccgtgga ttccatcgaa aaaactttgt ctgtgatcaa aaattctgat
     2101 ttctgttgac cgttgaaact ccgtataaac cgcgcaccct gaaaccgggg gtcggtgacc
     2161 gtggtgtccg gcccggaccg aaggttctgc tgcagcagcg tgcaggagtg acttgaacat
     2221 gcgtgaaaac ttcagcgaag acgaggaaag cttggacgac gacacggaac acgagtcgcc
     2281 ggccgtggtg ccgacgcccc cgcccgcgcc gtcgggcccc ggctccaagg cctggcgatc
     2341 catcgagcgg catcgcgaac tgcgcgaact tcgccgccac ctcgacgact tccagttcga
     2401 ggacgatccc aagtacgacc cgaggaacct gccgctctga tccggcacgc aaccgttttc
     2461 ccccgctgaa cccgcccggg cggtgcaggc aactgcaccg cccggagtgt ttcggggcag
     2521 gatcggggtg gcagaggtgc tttgacgcgg cgcgtttaac gcgaaacggt ataagctgtc
     2581 gatgatcgcg tcattcaggg acaaggacac ctgccggctc atggagggcg agcccgtgcc
     2641 ccgccttgca gcctgccgga aagccgcctt gcgcaagctc gacatgctcg cggctgccca
     2701 tgtccttcag gatttgcggc aaccaccggg taaccgtctg gaagccttgc agggtaaccg
     2761 ggccggacag cacagcatcc ggatcaac
//
LOCUS       DAOQJH010000388         2786 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_25115_length_2786_cov_2.623947, whole genome shotgun sequence.
ACCESSION   DAOQJH010000388 DAOQJH010000000
VERSION     DAOQJH010000388.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2786)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2786)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2786
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25115_length_2786_cov_2.623947"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..805
                     /gene="nusB"
                     /locus_tag="PLQ18_09175"
     CDS             <1..805
                     /gene="nusB"
                     /locus_tag="PLQ18_09175"
                     /inference="COORDINATES: protein motif:HMM:NF013216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HNE01865.1"
                     /translation="WQKGGQDITRIEAQFLGDLGVHRLMVALEARLAALPAAQSEVAA
                     ATGALDDAAAGEQLRVLRIAAERGARALPGGREGLADPLAVQGAVVQELAAVPNPPAP
                     AVAAALGLELAIRRLLRQLMQELEARGAYGADMAYLNGLNERELLGRESVTPDAARLN
                     EAVSEVVLADRAMLKADTGYFSTLLRGIPDRLDALNRLLAPWLGRPADQVDPIEKAIL
                     WIAAWELTESKDVPWRVAINEAIELAKRFGAEQSHKFVNGVLDKIVQQR"
     gene            complement(871..1554)
                     /locus_tag="PLQ18_09180"
     CDS             complement(871..1554)
                     /locus_tag="PLQ18_09180"
                     /inference="COORDINATES: protein motif:HMM:NF038353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxLYD domain-containing protein"
                     /protein_id="HNE01866.1"
                     /translation="MPSGATPHGLPQPFKDSSMRPLLLILPLTLASTAVAACAQDLPN
                     PDSAIEVVNASISTFRTEGPLLTTVIGELHNTTNDRIDELVIEARLTDASGKLIDVIS
                     EPVYGLVAPAGQNVAFRLQAQAAAAAEAYAGVSVRVISAQTHPPAPARARAPEPSRIP
                     DWLMAWGPMLLLIVVWLLLARKASSKGSFQERMLAGMQEQNALLGRQVSALEAIASKS
                     GTADKADGG"
     gene            2297..2686
                     /locus_tag="PLQ18_09185"
     CDS             2297..2686
                     /locus_tag="PLQ18_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018161791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNE01867.1"
                     /translation="MSAFTTACLPPETLELAPDGSECRPLVRLAGASCAHFRLAAGRC
                     SRAVEHRSVEEIWYVLDGRGELWRRQGTRAEVVALAAGVCVTIPLGTAFQFRAAAEAA
                     LSFVAVTLPPWPGEQEARLVEGGWPLD"
BASE COUNT          434 a          923 c          972 g          457 t
ORIGIN      
        1 gtggcagaag ggcgggcagg acatcacccg catcgaggcg cagttcctcg gcgatctcgg
       61 cgtgcaccgg ctgatggtgg cgctcgaagc ccggctcgcc gcgctgccgg cggcccagtc
      121 cgaggttgcc gcggccaccg gagccctcga cgatgccgcg gccggcgagc agctgcgcgt
      181 gctgcgcatc gctgccgagc gcggcgcgcg cgccctgccc ggaggccgcg agggactggc
      241 cgatccgctg gccgtgcagg gcgccgtcgt gcaggaactc gccgccgtgc cgaatccgcc
      301 ggccccggcc gtggccgccg cactcggcct cgaactcgcg atccggcgac tgctgcgcca
      361 gctgatgcag gaactcgaag cccgcggagc ctacggcgcc gacatggcgt atctgaacgg
      421 cctcaacgaa cgcgagctgc tcgggcgcga gtcggtgacg ccggatgccg cccgcctgaa
      481 cgaggcggtc agcgaggtcg tgctcgccga tcgcgccatg ctgaaggccg ataccggcta
      541 tttcagcacg ctgctgcgcg gcatccccga tcgcctcgat gcgctgaacc gcctgctcgc
      601 gccctggctc ggccgcccgg ccgatcaggt cgatccgatc gagaaggcca tcctgtggat
      661 cgccgcctgg gagctcaccg agtcgaagga cgtgccctgg cgcgtcgcga tcaacgaggc
      721 gatcgaactg gcgaagcgct tcggcgccga gcagagccac aagttcgtca acggcgtgct
      781 cgacaagatc gtgcagcagc gctgaagccc ggcgggccgg gccgggcggc gcggcctcga
      841 ccaccgcccg ccagcggccg acgccaagcc tcagccgccg tcggccttgt cggcggtgcc
      901 ggacttcgag gcgatcgctt cgagcgcgct gacctgacgg cccagcagcg cgttctgctc
      961 ctgcatgcct gccagcatcc gttcctgaaa cgaacccttg ctgctggctt tgcgggccag
     1021 cagcagccag accacgatca gcagcagcat cggcccccag gccatcagcc agtccgggat
     1081 tctggatggc tccggcgccc tggcgcgtgc tggcgctggc ggatgcgtct gcgcactgat
     1141 gacgcgcacg ctcacgcccg catacgcctc ggccgccgcc gccgcctgtg cctgcagacg
     1201 gaacgcaaca ttctggcctg cgggcgcgac caggccgtac accggctcgc tgatcacatc
     1261 gatgagcttg ccgctcgcat ccgtcagccg ggcttcgatg acgagttcat cgatcctgtc
     1321 gttcgtggtg ttgtgcagtt caccgatcac ggtggtcagc agcggccctt cggtcctgaa
     1381 ggtcgagatc gaggcattga cgacctcgat ggcgctgtcg gggtttggca ggtcctgggc
     1441 acaggcggcg acggcagtgc tcgcgagcgt gagcggcagg atcaggagca gggggcgcat
     1501 ggaggagtcc ttgaatggct ggggcaatcc atggggtgtt gcgccggatg gcacgccggc
     1561 acgatcgcca tgcaggcgca ggaaccggca ggtatcagga gggaaatccg gccgccacgc
     1621 tgcccgcccg cggcttcagg cgctgcgctt tgcagggacg ggcgggcgat cagtccccag
     1681 gggaccggca ccggatccgg aatctccgcg ggcagcatgc aaaccggcgt ctgacggcgt
     1741 caagcacgcg gctttcccgt ccggcgacgc aggccagccg tggaaatctg cgcatttcca
     1801 ttccagccct gcctctttcc agtggaaatg cgcgagcttc gaaggcaaac cgacaggaga
     1861 tgcacggaaa tattgcacat cgaagccttg ccgcacctga ttggagcatg atgtcgtgca
     1921 tttctcatgg aaagatgcgg gattcgatgg caatgttgcg agactgcaac ggaaatgtct
     1981 gcatttccat cgtgatcttg cggatttcca ctggaaagat acgtgctccg atgggaaacg
     2041 ggctcgccat gcaggaggct gcacgcattt gaagcctgtt tgcacactga tggtgcgcga
     2101 agctgtggca ttcatgccgt ccaccggctg atgccgatca caggtgcagg cgctgttgat
     2161 ctccctctcc ccaaatcctt cctcgcgagg gggaggggct gaaacgatcg ggggttcagc
     2221 ggtgatggcc atgaaaagcc tgcgatggcc gggagactgg cggccagact gacggcattc
     2281 ctgccggagt ctgcccatgt ccgcgttcac cactgcctgt ctgccgcccg aaaccctcga
     2341 actcgccccc gatggctcgg agtgccggcc gctcgtgcgg ctcgccgggg ccagttgcgc
     2401 gcatttccgc ctcgcggccg ggcgctgttc gcgcgcggtc gagcatcgga gcgtcgagga
     2461 gatctggtac gtgctcgatg gtcgcggcga gctgtggcgc cgccagggca cgcgggcgga
     2521 agtcgtcgcg ctcgcggccg gcgtctgcgt gacgatcccg ctcggcacgg ccttccagtt
     2581 tcgcgccgcc gcggaggcag ccctcagctt cgtcgccgtg accttgccgc cgtggccggg
     2641 ggagcaggag gcgcggctgg tggagggagg ctggccgctg gactgatgcc gttcgctccg
     2701 gaatgcctcg cttttcagtt cttcgcccgc cgtgggagag gggttgggga gagtgtgaga
     2761 aaaaacatgc agttacgtgg cgtcgg
//
LOCUS       DAOQJH010000389         2784 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_25139_length_2784_cov_4.056797, whole genome shotgun sequence.
ACCESSION   DAOQJH010000389 DAOQJH010000000
VERSION     DAOQJH010000389.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2784
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25139_length_2784_cov_4.056797"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            235..1092
                     /locus_tag="PLQ18_09190"
     CDS             235..1092
                     /locus_tag="PLQ18_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019752261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNE01868.1"
                     /translation="MLRFEGFAASRAAGGPQRLPALLLLGLLALARALPAAELEVGGP
                     AGSRRLDSAVLLATPATQDIDVPDDVAYGRPMRFRAVPLAPLLAAAGFTAADTVEAVA
                     LDGYVAQLPAAALLSDAADGAQAWLAIEPPDAPWPKLPRGEGTAGPFYLVWQEPQAGG
                     ITPEQWPFQLAVLRRVAAPAERWPALNPPAGAGSDAQRGLAVFVQHCLPCHRLNGAGE
                     ATIGPDLNRPMSPVEYFRPDLLRRYLREPAALRTWPGQGMPGFAESVLPAADLEALLA
                     YLAAMAAAR"
     gene            complement(1154..2536)
                     /locus_tag="PLQ18_09195"
     CDS             complement(1154..2536)
                     /locus_tag="PLQ18_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003271013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutB"
                     /protein_id="HNE01869.1"
                     /translation="MPYAHAFGATRHRFADLKDLLAKATPLRSGDVLAGLAAETAEQR
                     VAAQYALAELPLATFLNEAVIPYEDDEVTRLILDSHDAAAFAPVAHLTVGDFRDWLLS
                     AQADAGTLAALAPGLTPEMVAAVSKLMRVQDLIVVARKCRVVTRFRNTIGLPGRLSTR
                     LQPNHPTDDAAGIAATLLDGLLYGSGDAVIGINPATDNLTQVTGLLHLLDAVIHEYRI
                     PTQACVLTHVTTTLAAIGRGVPVDLVFQSIAGTEAANAGFGVTLALLDEAHQAALALQ
                     RGPLGDHVMYFETGQGSALSAGAHHGVDQQTCEVRAYAVARRFRPLLVNTVVGFIGPE
                     YLYDGKQILRAGLEDHCCGKLLGLPMGCDVCYTNHAEADQDDMDALLTLLGTAGVTYV
                     MGIPGADDIMLNYQTTSFHDALALRATLGLRPAPEFEDWLYASGVFGADGRPAEALPA
                     AFAAALARIG"
BASE COUNT          344 a          983 c         1056 g          401 t
ORIGIN      
        1 cggcccgggg caccttgccg gaccctgccg tgcctgccgc tcagccggga aggcagggtc
       61 tttcttccgc tctccccgat cccgcttccc ccgccgggcg gcgggttttt tgtcgatact
      121 tgccgtggtg tgtcgtcgtc ctgatccggc agtggttccc ggactcgtcc gggagccgct
      181 gcgtccggga gtggtgcaga taccgatgcc aggggaacag ggggagggat ggtcatgctg
      241 cgcttcgaag ggttcgcggc gagccgtgcc gcgggaggcc cgcagcggct gccggcgctg
      301 ctgctgctcg gcctgctggc gctggcgcga gcgctgccgg cggccgagct tgaagtcggc
      361 ggcccggccg gcagccggcg gctcgacagc gccgtgctgc tcgccacgcc ggcgacgcag
      421 gacatcgacg tgcccgacga tgtcgcctac ggccggccga tgcgctttcg cgccgtgccg
      481 ctcgcgccgc tgctggcggc ggccggcttc acggccgcgg acacggtcga ggcggtcgcg
      541 ctcgacggct acgtcgcgca gctgccggcc gcggccctgc tcagcgacgc cgccgacggc
      601 gcgcaggcct ggctcgcgat cgagccgccg gatgcaccgt ggccgaagct gccgaggggc
      661 gagggcacgg ccgggccgtt ctatctggtc tggcaggagc cgcaggccgg cggcatcacg
      721 cccgagcagt ggccgttcca gctcgccgtg ctgcgccggg tcgcggcccc ggccgaacgc
      781 tggccggcgc tgaatccgcc cgccggtgcg ggcagtgacg cgcagcgcgg actcgcggtg
      841 ttcgtgcagc actgcctgcc ctgccaccgc ctgaacggtg ccggcgaggc gacgatcggc
      901 ccggacctga accggccgat gagtccggtc gaatacttcc gccccgatct gctgcgccgt
      961 tacctgcgcg agccggcggc gctgcgcacc tggccggggc agggcatgcc gggcttcgcc
     1021 gagagcgtgc tgccggcggc cgatctcgaa gcgctgctcg cgtatctcgc ggcgatggcg
     1081 gcggcacgct gagcgccgcc ggcgatgcgg tcgcggccgg acgccatgca gttggccggc
     1141 cgtcgccggg ggttcagccg atccgcgcca gcgccgcggc gaaggctgcc ggcagcgcct
     1201 cggccgggcg gccgtcggcg ccgaacacgc cgcttgcgta cagccagtcc tcgaactccg
     1261 gcgccgggcg caggccgagc gtcgcgcgca gcgccagcgc gtcgtgaaac gaggtggtct
     1321 ggtagttcag catgatgtcg tcggcgccgg ggatgcccat cacgtaggtg acgccggcgg
     1381 tgccgagcag cgtcagcagc gcgtccatgt cgtcctggtc ggcttcggcg tggttcgtgt
     1441 agcagacatc gcagcccatc ggcaggccga gcagcttgcc gcagcagtga tcctccagcc
     1501 cggcgcggag gatctgcttg ccgtcgtaga ggtactccgg gccgatgaag ccgacgacgg
     1561 tgttgaccag cagcggccgg aagcggcggg cgaccgcgta ggcgcgcacc tcgcaggtct
     1621 gctggtcgac gccgtggtgc gcgccggccg acagcgccga gccctggccg gtctcgaagt
     1681 acatgacgtg gtcgccgagc gggccgcgct gcagcgccag cgccgcctga tgcgcctcgt
     1741 cgagcagcgc gagcgtcacg ccgaagccgg cgttggcggc ctcggtgccg gcgatcgact
     1801 ggaagaccag atcgaccggc acgccgcgcc cgatcgccgc gagcgtcgtc gtcacgtgcg
     1861 tcagcacgca ggcctgggtc gggatgcggt attcgtggat caccgcatcg agcagatgca
     1921 gcagcccggt gacctgcgtc aggttgtcgg tggccgggtt gatgccgatc accgcgtcgc
     1981 cgctgccgta gagcaggccg tcgagcaggg tggcggcgat gccggcggca tcgtcggtcg
     2041 ggtggttcgg ctgcagccgc gtcgacagcc gcccgggcag gccgatggtg ttgcgaaagc
     2101 gcgtgaccac gcggcacttg cgcgcgacga cgatcagatc ctgcacgcgc atcagcttgc
     2161 tgaccgcggc gaccatctcc ggcgtcagcc ccggcgcgag cgcggcgagc gtgccggcat
     2221 cggcctgcgc cgacagcagc cagtcgcgga aatcaccgac cgtcaggtgc gcgaccggcg
     2281 cgaaggccgc ggcatcgtgg ctgtcgagga tcaggcgcgt gacctcgtca tcctcgtacg
     2341 ggatcacggc ctcgttcagg aaggtcgcga gcggcagctc ggcgagcgcg tactgcgcgg
     2401 cgacgcgctg ctcggccgtt tccgcggcga ggccggcgag cacgtcgccg gagcgcagcg
     2461 gcgtggcctt ggcgagcagg tccttcaggt ccgcgaagcg gtggcgcgtg gcgccgaagg
     2521 cgtgggcgta gggcatcggg agactcctcg cggcggctgc accgtggctc acgcccgggt
     2581 gctgttgccg ccagtctcgc cgcatcaggc gacgccatcc acccgtttca tgcccgccgg
     2641 aacaaggcgc cggtgcgccc gggagcacac ctcgacaggc ccgtcgcctt cctgccgcgc
     2701 agggaaggcg acggtccggc gcgcggcgct tactccttga cctcgacctt ggcgccggcc
     2761 ttgagctttt ccagatagtc gttg
//
LOCUS       DAOQJH010000390         2782 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_25175_length_2782_cov_5.305097, whole genome shotgun sequence.
ACCESSION   DAOQJH010000390 DAOQJH010000000
VERSION     DAOQJH010000390.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2782)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2782)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2782
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25175_length_2782_cov_5.305097"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2782)
                     /locus_tag="PLQ18_09200"
     CDS             complement(<1..>2782)
                     /locus_tag="PLQ18_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018006795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004352 - glutamate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019551 - glutamate catabolic process to
                     2-oxoglutarate [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase"
                     /protein_id="HNE01870.1"
                     /translation="FSQSYMEETLARHAPITGQLAALFVARFDPDHPDAARCEQLAGG
                     IESALENVAVLDEDRILRRFLAVILATLRTNFYQTSQPGQPGTDGGPKEYLSFKFDPA
                     KIPELPLPRPMFEIWVYSPRAEAIHLRGGRVARGGIRWSDRREDFRTEVLGLVKAQQV
                     KNAVIVPVGSKGGFVVKRPPAEREALMQEVVHCYKTLMRGMLDITDNLVHGEVVPPAR
                     VVRYDADDPYLVVAADKGTATFSDIANGLALEYGFWLGDAFASGGSAGYDHKKMGITA
                     RGAWESVKRHFRELGKDIQNVDFSVAGIGDMSGDVFGNGMLLSRHIRLIAAFDHRHVF
                     IDPSPDAEASFAERERLFALPRSSWADYDAALISAGGGIWPRTAKSIPLSPEMRAILG
                     TDATRLSPAELINTILRAPVELLWNGGIGTYVKAADETHAEVGDRANDAIRVNGSELR
                     CKVVGEGGNLGFTQRGRIEYARAGGHILTDAIDNSAGVNCSDHEVNIKILLNQIVSAG
                     DLTLKQRNELLATMTDEVARLVLRQNVLQPQTISIAVREAPDLLGDHARVIRQLEKAG
                     RLNRRIEFLPPDDEIAEREQAGQGLTAPEIAVLLAYSKIALYDELLDSDVPEDPYLER
                     ELRGYFPEPLRERYAEAMERHPLRRQIIATAITNSMLNHMGSTFAFRVNEQCGARSPD
                     IARAYAAARAMFEAPRLWAAIDALDNRAAATLQLDMISDTQALLMRASLWLLRNRRPP
                     IAIEAEVARFAPDITRLLSMKNLMPGRAGATLQAEAARLEGHGVPAETAEWVASLPAL
                     YCTLDIIEVANQAQIAAEQVALVYFTLIDALELDWLAATIDRLGAGNHWQHRARSALL
                     DNLYTQQRRLAAAVVAERGPEEHPQPWIDAWLAHNHAAVERLAAVLTDLKSAPRVDSS
                     MLMVALGEVGVGA"
BASE COUNT          381 a          934 c         1029 g          438 t
ORIGIN      
        1 gcgccgaccc cgacctcccc gagcgccacc atcagcatcg acgaatcgac gcgcggggcg
       61 gacttgaggt cggtgagcac cgcggcgagg cgttcgaccg cggcgtggtt gtgggcgagc
      121 caggcgtcga tccacggctg cgggtgttcc tccggcccgc gctcggcgac gacggcggcc
      181 gcgaggcggc gctgctgggt gtagaggttg tcgagcagcg cgctgcgggc gcggtgctgc
      241 cagtggttgc cggccccgag gcggtcgatg gtcgcggcca gccagtcgag ttcgagcgcg
      301 tcgatcagcg tgaagtacac gagcgccacc tgctcggcgg cgatctgcgc ctgattggcg
      361 acctcgatga tgtcgagcgt gcagtacagc gccggcaggc tcgcgaccca ctcggcggtc
      421 tcggccggca cgccgtggcc ttcgaggcgc gccgcctcgg cctgcagcgt cgcaccggcg
      481 cggcccggca tcaggttctt catcgacagc aggcgcgtga tgtcgggcgc gaagcgcgcg
      541 acctcggcct cgatcgcgat cggcggccgg cggttgcgca gcagccacag cgaggcgcgc
      601 atcagcagcg cctgcgtgtc cgagatcatg tcgagctgca gcgtcgcggc ggcgcgattg
      661 tcgagcgcgt cgatcgctgc ccacagcctg ggggcctcga acatcgcgcg cgccgcggcg
      721 taggcgcggg cgatgtccgg cgagcgcgcg ccgcactgct cgttgacgcg gaacgcgaag
      781 gtcgagccca tgtggttcag catcgagttc gtgatcgccg tggcaatgat ctggcgccgc
      841 agcgggtggc gttccatcgc ctcggcgtag cgctcgcgca gcggctcggg gaagtagccg
      901 cgcagctcgc gttcgaggta ggggtcctcg ggcacgtcgg aatcgagcag ctcgtcgtac
      961 agcgcgatct tcgagtacgc gagcagcacg gcgatttccg gcgccgtcag gccctgaccg
     1021 gcctgctcgc gttcggcgat ctcgtcatcg ggcggcagga actcgatgcg ccggttcagc
     1081 cggccggcct tttcgagctg gcggatcacg cgcgcgtggt cgccgagcag atccggcgcc
     1141 tcgcgcaccg cgatcgagat cgtctgcggc tgcagcacgt tctggcgcag cacgaggcgc
     1201 gcgacctcgt cggtcatcgt cgccagcagc tcgttgcgct gcttcagcgt caggtcgccg
     1261 gcgctgacga tctggttcag caggatcttg atgttgacct cgtgatccga gcagttgacg
     1321 ccggccgagt tgtcgatggc atcggtcagg atgtggccgc cggcgcgcgc gtactcgatg
     1381 cgtccgcgct gcgtgaagcc gaggttgccg ccctcgccga ccaccttgca gcgcagctcg
     1441 ctgccgttga cgcggatcgc gtcgttggcg cggtcgccga cctcggcgtg cgtctcgtcg
     1501 gcggccttga cgtaggtgcc gatgccgccg ttccacagca gctcgaccgg cgcacgcagg
     1561 atggtgttga tgagttccgc cggcgacagg cgcgtcgcgt cggtgccgag gatggcgcgc
     1621 atctcggggc tgagcgggat cgacttggcc gtgcgcggcc agatgccgcc gccggccgag
     1681 atcagcgcgg cgtcgtagtc ggcccaggac gagcgcggca gcgcgaacag ccgctcgcgc
     1741 tcggcgaagc tcgcctcggc gtccggcgac gggtcgatga acacgtggcg gtggtcgaag
     1801 gccgcgatca ggcggatgtg gcgcgacagc agcatgccgt tgccgaacac gtcgccgctc
     1861 atgtcgccga tgccggcgac cgagaagtcg acgttctgga tgtccttgcc gagttcgcgg
     1921 aagtggcgct tgaccgattc ccaggcgccg cgcgcggtga tgcccatctt cttgtggtcg
     1981 tagccggccg agccgccgga ggcgaaggca tcgccgagcc agaagccgta ttcgagcgcc
     2041 agaccgttgg cgatgtccga gaaggtcgcc gtgcccttgt cggcggcgac caccagatac
     2101 gggtcatcgg cgtcgtagcg cacgacgcgc gccggcggca cgacctcgcc gtgcacgagg
     2161 ttgtcggtga tgtcgagcat gccgcgcatc agcgtcttgt agcagtgcac gacctcctgc
     2221 atcagcgcct cgcgctcggc cggcgggcgc ttgacgacga agccgccctt ggagccgacc
     2281 ggcacgatca cggcgttctt gacctgctgc gccttcacga gcccgagcac ctcggtgcgg
     2341 aagtcctcgc gccggtccga ccagcggatg ccgccgcgtg cgacgcggcc gccgcgcagg
     2401 tggatggcct cggcgcgcgg cgagtacacc cagatctcga acatcggccg cggcagcggc
     2461 agctccggaa tcttcgccgg gtcgaacttg aacgacaggt attccttcgg cccgccgtcg
     2521 gtgccgggct ggccgggctg gctggtctgg tagaaattgg tgcgcagcgt cgcgaggatc
     2581 accgcgagga agcggcgcag gatgcggtcc tcatcgagca cggcgacgtt ttcgagcgcc
     2641 gactcgatgc cgccggcgag ctgttcgcag cgcgccgcgt ccgggtgatc gggatcgaag
     2701 cgcgcgacga acagcgccgc gagctggccg gtgatcggcg cgtggcgcgc cagcgtctcc
     2761 tccatgtagc tctgcgagaa cg
//
LOCUS       DAOQJH010000391         2780 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_25197_length_2780_cov_4.602202, whole genome shotgun sequence.
ACCESSION   DAOQJH010000391 DAOQJH010000000
VERSION     DAOQJH010000391.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2780)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2780)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2780
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25197_length_2780_cov_4.602202"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(101..1075)
                     /locus_tag="PLQ18_09205"
     CDS             complement(101..1075)
                     /locus_tag="PLQ18_09205"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018746943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HNE01871.1"
                     /translation="MPFAGLDPDCILDAVEAAGHRCDGRLLALNSYENRVWQVGREDD
                     TPLVAKFYRAGRWRDAQILEEHAWSADLAAAELPVVAPLAAADGTTLFRHRGFRYALY
                     PRHGGRWPPLDDPETLYRLGRLLGRIHAHGAQLPFRERPALDVERFGHEPRATVLAGS
                     WLPAYLREAYATLSRGLLAAIEAAFAGLRWRSIRLHGDCHPGNLLWTDEGCHFVDLDD
                     AVSGPAVQDLWMLLSGERDERQLQLAELIEGYREFHDFDARELALIEPLRALRQLHYA
                     AWIARRWDDPAFPAAFPWFAGPRYWEEHVLALRECLAALGEPPLSLWD"
     gene            complement(1081..1665)
                     /locus_tag="PLQ18_09210"
     CDS             complement(1081..1665)
                     /locus_tag="PLQ18_09210"
                     /inference="COORDINATES: protein motif:HMM:NF015858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajG family lipoprotein"
                     /protein_id="HNE01872.1"
                     /translation="MRLSAVLRRSLLCAVLAVAGGCALSPQTLNIHPSLDVAAPPLGR
                     GRPLMLEVIDARTQPAVFGTRGGVYQTATVGPRNDVAAAVRQALAERLTAAGFVLSAP
                     QPGSRTLRVELLRIDYHASGEPAVGEVRTSCEVRAIAGNAASSYSGNYRANSVRQVVG
                     SPGVEDNEQIVNEIISQALQRMLADRGLLETLAR"
     gene            1822..>2780
                     /locus_tag="PLQ18_09215"
     CDS             1822..>2780
                     /locus_tag="PLQ18_09215"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HNE01873.1"
                     /translation="MADDCAIALGQSHLNLYQRIAEDLATAIANGEHVPGERVPSVRE
                     LARRLGVSISTVTQAYGLLVDRGVLEARPQSGYYVGLRARASAPEPSKSRPAVQPMPV
                     STSALTLEVLAGAGDPGLINLGNAVAAPDLLPTASINRCLMSVIREMPELSAVYDAPF
                     GRPELRAQVVRLAHGAGVRAAPEEVLITGGCQEGVTLCLRALARPGDTIAVESPTFFG
                     TLQAIESLGMHALEIPTHPRTGICLDALEQALAQWPIRACVVMPTNANPVGSCMELAE
                     RQKLVELLARHEIPLIEDDACGDLGFAPGPRPPACKAWDRDGGV"
BASE COUNT          427 a         1010 c          973 g          370 t
ORIGIN      
        1 gctacggctc ggatcacatc ctgaccgcgg cgaccgaggt gatcggcgaa ctcggtcgcg
       61 acatcggcaa cagccggaaa gccggaacgg tcgccgacga ctagtcccac agcgacaagg
      121 gcggctcgcc gagcgcggcg agacactcgc gcagcgccag cacgtgctcc tcccagtagc
      181 gcggtccggc gaaccacgga aaggccgccg ggaaggccgg atcgtcccag cgccgggcga
      241 tccaggcggc gtagtgcagc tggcgcagcg cgcgcagcgg ctcgatcagc gccagctcgc
      301 gcgcatcgaa gtcgtggaac tcgcgatagc cctcgatcag ttcggcgagc tgcagctgac
      361 gctcgtcgcg ctcgccggac agcagcatcc agagatcctg cacggccggg ccgctgacgg
      421 cgtcgtcgag atcgacgaag tggcagcctt cgtcggtcca gagcaggttg ccggggtggc
      481 agtcgccgtg cagccggatc gaccgccagc gcaagcccgc gaaagcggcc tcgatcgcgg
      541 cgagcaggcc gcggctgagc gtggcatagg cctcgcgcag atacgccggc agccaggacc
      601 cggcgagcac cgtcgcgcgc ggctcgtgac cgaagcgttc cacgtcgagc gccggccgct
      661 cgcgaaacgg cagttgcgcg ccatgcgcgt ggatgcgccc gagcaggcgg ccgagccggt
      721 acagcgtctc cgggtcatcg agcggcggcc agcggccgcc atggcgcgga tacagcgcat
      781 aacggaagcc ccggtgacgg aacagcgtcg tgccatcggc cgccgcgagc ggcgcgacca
      841 ccggcagctc ggccgcggcg aggtcggcgc tccaggcgtg ttcttcgagg atctgcgcat
      901 cgcgccagcg cccggcccgg tagaacttcg cgaccagcgg cgtgtcgtcc tcccgaccca
      961 cctgccagac ccggttctcg tagctgttca gcgccagcag ccggccgtcg cagcgatggc
     1021 cggcggcttc gacggcgtcg aggatgcagt ccggatcgag accggcaaac ggcagacggt
     1081 tcaccgggcg agtgtttcga gcaggccgcg atcggcgagc atgcgctgca gggcctggct
     1141 gatgatctcg ttgacgatct gctcgttgtc ctcgactccg ggcgagccga ccacctggcg
     1201 cacgctgttc gcgcggtagt tgccgctgta gctgctggcg gcattgccgg cgatggcgcg
     1261 cacctcgcaa ctggtgcgga cctcgccgac cgccggctcg ccgctggcgt gatagtcgat
     1321 gcgcagcagc tcgacgcgca gcgtgcggct gccgggctgc ggcgccgaca gcacgaagcc
     1381 cgctgccgtc agccgttcgg cgagcgcctg gcgcacggcc gccgccacgt cgttgcgcgg
     1441 cccgaccgtg gcggtctgat aaaccccgcc gcgggtgccg aacaccgccg gctgtgtgcg
     1501 cgcatcgatg acttcgagca tgagcggccg gccgcgaccg agcggcggcg cggccacgtc
     1561 gagcgaggga tggatgttca gggtctgcgg actcagtgcg cagccgcccg ccacggcgag
     1621 gacggcacac agcaggctac gacgcagaac ggcggacagg cgcatgcgac cgactccgga
     1681 gcgtgttgac cgcgtgcagc ataccgcaga gcaccaccgc tgccggacgc cgtgttcacc
     1741 gctgctgaca cggccggggc tgcgccgcac agcacagtct gcaagaatgt cgtccatcac
     1801 cagcacagac gatgcttgat catggccgac gactgcgcca tcgccctcgg gcaatcccat
     1861 ctgaacctgt accagcgcat cgccgaggat ctggccaccg cgatcgccaa tggcgagcac
     1921 gtccccggcg agcgcgtgcc ctcggtgcgc gagctggcgc gccggctcgg cgtcagcatc
     1981 tcgacggtca cccaggccta cggcctgctg gtcgatcgcg gcgtgctcga agcccggccg
     2041 cagtccggct actacgtcgg cctgcgcgca cgcgccagcg caccggaacc gtccaagtcc
     2101 cgcccggccg tgcagccgat gccggtgtcc accagtgcgc tgacgctcga agtgctggcc
     2161 ggggccggcg atccggggct catcaacctc ggcaacgcgg tcgccgcgcc cgacctgctg
     2221 ccgactgcgt cgatcaaccg ctgcctgatg tcggtgatcc gcgagatgcc ggagctgtcg
     2281 gcggtctacg acgcgccctt cggccgcccg gagctgcgtg cgcaggtcgt gcgcctcgcg
     2341 catggcgccg gcgtgcgcgc agcccccgag gaagtcctga tcaccggcgg ctgtcaggaa
     2401 ggcgtgacgc tctgcctgcg cgcgctcgcc cgccccggcg acacgatcgc ggtcgaatcg
     2461 ccgaccttct tcggcacgct gcaggcgatc gaatcgctcg gcatgcacgc cctggagatc
     2521 ccgacccatc cgcgcaccgg catctgtctc gacgccctcg aacaggcgct cgcgcagtgg
     2581 ccgatccgcg cctgcgtcgt gatgccgacc aacgccaatc cggtcggctc gtgcatggag
     2641 ctggcggaac gccagaagct cgtcgaactg ctcgcgcgtc acgagatacc gctgatcgag
     2701 gacgatgcct gcggcgacct cggcttcgca cccggcccgc ggccaccggc ctgcaaggcc
     2761 tgggaccgcg acggtggcgt
//
LOCUS       DAOQJH010000392         2766 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_25425_length_2766_cov_5.308373, whole genome shotgun sequence.
ACCESSION   DAOQJH010000392 DAOQJH010000000
VERSION     DAOQJH010000392.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2766)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2766)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2766
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25425_length_2766_cov_5.308373"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(10..945)
                     /gene="xerD"
                     /locus_tag="PLQ18_09220"
     CDS             complement(10..945)
                     /gene="xerD"
                     /locus_tag="PLQ18_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HNE01874.1"
                     /translation="MPEQDAGALTAIVPATEETAIEAYIDEVWMEMGLSTNTLAAYRA
                     DLRACAGWLSLRRCGMTLLQAGAADLQAYLAARHTLSPRSLARLQSALRHYYRHQLRH
                     GRIAVDPTGVIETPKLGRRLPGSLSEAEVEALLAAPDAGTSLGLRDRAMLELLYATGL
                     RVSELVTLGRDQVNPRGGVLRVSGKGNKERLVPIGETALDWLERYLAEARPALLGMRL
                     TEALFVTSRGESMTRQNFWYLIKRYAMTAGIDPMRLSPHTLRHAFATHLVNHGADLRV
                     VQLLLGHSDLSTTQIYTHVARERLKRLHERHHPRG"
     gene            986..1513
                     /gene="mobB"
                     /locus_tag="PLQ18_09225"
     CDS             986..1513
                     /gene="mobB"
                     /locus_tag="PLQ18_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006748889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HNE01875.1"
                     /translation="MLHPSVRPCLGFAACSGTGKTTLLLELIPRLVAAGLRLGLAKHS
                     HHALDLDRTGKDSQRLRMAGAAQVVVAAPRRLARIEACSRNLAECLALLARAPAAPDL
                     ILVEGWKHAPIPRLELHRPALGQPLLAPADPLILAVACDAPPHTPAGVPLLPLGDADA
                     IAACVLGWLAAGRLG"
     gene            1565..2434
                     /locus_tag="PLQ18_09230"
     CDS             1565..2434
                     /locus_tag="PLQ18_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012823357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNE01876.1"
                     /translation="MTPSASNPKRALSVLVTGCSSGIGHCAAHGLRARGHRVFAAARR
                     SADVERLRAEGLEAVMLDLDDDAGIGSGLDAVLDATGGRLDALFNNGAYGQPGAVEDL
                     TRAALREQFETNLFGTAVLTNRVIPVMRRQGHGRIVMNSSLLGYAAMAYRGAYNASKF
                     ALEGLTDTLRLELAGSGIHVSLIEPGPITSRFRANAEAAFHRHIDPAGSVHRAAYVAM
                     AERLARPGPAAPFTLGPEAVLDKLVHALESPHPRRRYPVTLPAHAFWWLRRLLPFCLI
                     DRILAAAGGSGRR"
     gene            complement(2536..>2766)
                     /locus_tag="PLQ18_09235"
     CDS             complement(2536..>2766)
                     /locus_tag="PLQ18_09235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE01877.1"
                     /translation="PHVKVGHRQAPIPDTPIHEWIGVSPFRSTEIRRRANPQSKAEAV
                     FRRQSRRISANLAAQTSLLPFFPRIPCEWSRA"
BASE COUNT          404 a          994 c          927 g          441 t
ORIGIN      
        1 ggcccgccgc tagccgcgcg ggtggtggcg ctcgtgcagg cgcttcaggc gctcgcgcgc
       61 gacgtgcgtg tagatctgcg tcgtcgacag atccgaatgc ccgagcagca gctgcacgac
      121 gcgcaggtcg gcgccgtggt tgaccagatg cgtcgcgaag gcatggcgca gcgtatgcgg
      181 tgacaagcgc atcgggtcga tgccggcggt catcgcgtag cgcttgatca ggtaccagaa
      241 gttctgccgc gtcatcgatt cgccgcgcga ggtcacgaac agcgcctcgg tgagccgcat
      301 gccgagcagc gccggacggg cctcggcgag atagcgctcc agccagtcga gcgcggtctc
      361 gccgatcggc accagccgct ccttgttgcc cttgccgctg acgcgcagca caccgccgcg
      421 cgggttgacc tgatcgcgac cgagcgtgac cagctcgctg acgcgcaggc cggtcgcgta
      481 cagcagttcg agcatggcgc gatcgcgcag cccgagcgag gtgccggcat ccggcgccgc
      541 cagcagcgcc tcgacttcgg cctccgacag cgagcccggc agccggcggc cgagcttcgg
      601 ggtttcgatc acgccggtcg ggtcgacggc gatgcggccg tggcgcagct ggtggcggta
      661 gtaatgccgc agcgccgact gcagccgcgc gaggctgcgc ggcgacagcg tgtggcgggc
      721 ggccagatag gcctgcaggt cagcggcgcc ggcctgcagc agcgtcatgc cgcagcgcct
      781 gagcgacagc cagccggcgc aggcgcgcag atcggcgcga taggccgcga gcgtgttggt
      841 gctcaggccc atctccatcc agacctcgtc gatataggcc tcgatcgccg tttcttccgt
      901 tgccggcacg atcgcggtca aggcgccggc atcctgctcc ggcatactgc cgattccgtc
      961 atccatctcc ctggtgctcc tgctgatgtt gcatccgtct gtgcgcccct gcctcggatt
     1021 cgccgcctgc agcgggacgg gcaagacaac gctgctgctc gaactgatcc cgcggctcgt
     1081 ggcggccggc ctgcggctcg gactcgccaa gcacagccac cacgccctcg acctcgaccg
     1141 taccggcaag gacagccagc ggctgcggat ggccggtgcc gcgcaggtgg tggtggccgc
     1201 accgcgacgg ctggcgcgca tcgaggcctg cagccgcaac ctggcggaat gtctggcgct
     1261 gctcgcacgc gcgccggccg ccccggacct gatcctcgtc gagggctgga agcatgcgcc
     1321 gattcccaga ctcgaactgc accggccggc actcggacag ccgctgctcg cccctgccga
     1381 cccgctgatc ctcgccgtcg cctgcgatgc gcccccgcac accccggccg gcgtgccgct
     1441 gctgccgctc ggcgatgcgg atgccatcgc cgcctgcgtg ctaggctggc tcgccgccgg
     1501 tcgcctcggg tgatgcatgg cagcataacg aactttcccg tcccgaccgt attgccggct
     1561 gagcatgacc ccatctgcat caaacccgaa gcgcgcactc agcgtcctcg tcaccggctg
     1621 ttcgagcggc atcggccatt gtgctgcgca cggcctgcgc gcacgcggtc accgcgtgtt
     1681 cgccgcggcg cggcgcagcg ccgatgtcga acggctgcgg gcggaaggac tggaagcggt
     1741 catgctcgat ctggatgacg atgccggcat cggctcgggt ctcgatgccg tgcttgacgc
     1801 caccggcggc cggctcgatg cgctgttcaa caatggtgcc tacggccagc ccggcgcggt
     1861 cgaggatctc acgcgcgccg ccctgcgcga gcagttcgaa accaacctgt tcggaaccgc
     1921 ggtgctgacc aatcgcgtga ttccggtcat gcgccgccag gggcacggcc gcatcgtcat
     1981 gaacagctcg ctgctcggct atgccgcaat ggcctaccgc ggcgcctaca acgccagcaa
     2041 gttcgcgctc gaaggcctga ccgacacgct gcgcctcgaa ctcgccggca gcggcatcca
     2101 cgtcagcctg atcgaacccg gcccgatcac cagccgcttc cgtgccaacg ccgaagccgc
     2161 cttccatcgc cacatcgacc cggcgggcag cgtgcatcgc gccgcctacg tggcgatggc
     2221 cgagcgcctc gccagacccg gcccggccgc cccgttcacg ctcggcccgg aagcggtgct
     2281 cgacaagctc gtgcatgcgc tcgaaagccc gcatccgcgt cgccgctatc cggtcacgct
     2341 accggcacat gcgttctggt ggctgcgccg gctgctgccg ttctgcctga tcgaccgcat
     2401 cctcgccgcc gccggcggca gcggcaggcg ctgaactgcg gttcagccgg tctgcttacg
     2461 acggttgcgg atctgccgat gaaccggctc ccgacaggaa gccggttttt tattgccggt
     2521 tgcggcaatt ccgcgtcaag ccctgctcca ttcgcaggga atgcgcggga agaagggcaa
     2581 aaggctcgtc tgcgctgcca ggtttgcgct gattcgcctg ctctgccggc gaaacaccgc
     2641 ttcggctttg gattgcggat tcgctcgccg ccggatttcg gtcgaccgaa aaggcgaaac
     2701 cccgatccat tcgtggatcg gggtatcggg gataggagcc tggcggtgac ctactttcac
     2761 atgggg
//
LOCUS       DAOQJH010000393         2762 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_25487_length_2762_cov_5.294052, whole genome shotgun sequence.
ACCESSION   DAOQJH010000393 DAOQJH010000000
VERSION     DAOQJH010000393.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2762)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2762)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2762
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25487_length_2762_cov_5.294052"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            125..2098
                     /locus_tag="PLQ18_09240"
     CDS             125..2098
                     /locus_tag="PLQ18_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020504866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNE01878.1"
                     /translation="MGAVSPAGLAASPLDELDPALRALVEGTAAVTGDAFFRSLVRGV
                     AHALGVRWAFVAEFADDHRFVRMLAYWDYDDFIAEETFELDGTPCERVLGGEPAFYPA
                     RVADLFPRNTDLRKMGAQSYLAIPLRTAAGAVLGHLAVLDDRPMAGQPRDLALFQVFG
                     ARAAAELERVRTERALREREARLGAILDSTLDAVLCLDGDRRVGLFNPAAEQVFRCRA
                     AEVLGQPVDRLLGADFLGVLQRYLQDGESAPLWLAQALDARRADGERFPAEATLSRAR
                     VDGRLLCTLILRDVNDRHQAEAALARLRQEKAYLQEQALGEAPDLIGRSAAMLAVRAQ
                     LDKVAATEAGVLLLGETGTGKELLARHLHRKSPRAAQLLVKMNCAALPSELIESELFG
                     HEKGAFSGAIASRKGRFELADGGTLFLDEVGELSLPAQAKLLRVLQEREFERVGGSRP
                     IRVDVRVVAATNRDLAQMVEAGLFRADLYYRLNVFPVRVPPLRERPEDIPLLVTHTLA
                     GLARRLGKPLHGVSPAARERLIAWSWPGNVRELQNVIERAAILASGDTIEVEDLGLMP
                     VSPGVALSPVTGLAAQPVPVEAAAAARPLPMAPAAEEGANLEAVERRHIEQVLTGCNG
                     VIEGPKGAAAALGLHPSTLRSRMQKLGMRGRRE"
     gene            2281..2739
                     /locus_tag="PLQ18_09245"
     CDS             2281..2739
                     /locus_tag="PLQ18_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013032605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE01879.1"
                     /translation="MKIYQQLMASLFAMIFQGLAFALILVAGWQFAGAWREGSEEVLS
                     IVVSGINTLVISLAMYELGVGVGKEYDPDNSPDNAYQTVRRTISRFVGTVCSALVLEG
                     LIMAIKYSQLDLAGNLVYPVALLISAAVLLIALGVFLRLTAPPAVQSEPR"
BASE COUNT          349 a          991 c         1006 g          416 t
ORIGIN      
        1 cggacctcat ggaagccggc ctgcacgagc acatggtctg gggtctgctg ctgctggtga
       61 cgatcgcgca cggcccgggc gcgctgtccg ccgatcgcct gctcggtcgc tggatcgccc
      121 ggcggtgggc gcagtgagtc cggccgggct cgccgcctcc ccgctcgacg agctcgatcc
      181 ggcgctgcgc gctctggtcg aaggcaccgc cgccgtcacc ggcgatgcct tcttccgcag
      241 cctggtgcgc ggtgtcgcgc acgcactcgg cgtgcgctgg gccttcgtcg ccgagttcgc
      301 cgacgatcac cgcttcgtgc gcatgctcgc gtactgggac tacgacgatt tcatcgccga
      361 ggagaccttc gagctcgacg gcacgccctg cgagcgcgtg ctgggcggcg aaccggcctt
      421 ctatccggcg cgcgtcgccg atctgttccc gcgcaacacc gacctgcgca agatgggcgc
      481 gcagagctat ctggcgattc cgctgcgcac cgcggccggc gccgtgctcg ggcatctggc
      541 ggtgctcgat gaccggccga tggccggaca gccgcgcgat ctggcgctgt tccaggtctt
      601 cggcgcccgc gccgcggcgg agctcgaacg cgtgcgcacc gagcgggcgc tgcgcgagcg
      661 cgaggcgcgc ctgggtgcga tcctcgattc gacgctcgat gccgtgctct gcctcgatgg
      721 cgatcggcgc gtgggcctgt tcaacccggc cgccgagcag gtgttccgct gccgcgccgc
      781 cgaggtgctc ggccagccgg tcgaccggct gctcggcgcg gacttcctcg gcgtgctgca
      841 gcgctatctg caggatggcg agagcgcgcc gctgtggctt gcgcaggcgc tcgatgcgcg
      901 gcgcgccgac ggcgagcgct tcccggccga ggcgacgctg tcgcgcgcgc gggtcgacgg
      961 gcggctgctc tgcacgctga tcctgcgcga tgtcaacgac cgccatcagg ccgaagccgc
     1021 gctggcgcgg ctgcgtcagg agaaggccta cctgcaggag caggccctcg gcgaggcccc
     1081 cgacctgatc gggcgctcgg cggcgatgct cgccgtgcgc gcccagctcg acaaggtcgc
     1141 cgccaccgag gccggcgtgc tgctgctcgg cgagaccggc accggcaagg aactgctcgc
     1201 ccgccacctg caccgcaaga gcccgcgcgc cgcgcagctg ctggtgaaga tgaactgcgc
     1261 ggcgctgccc tcggagctga tcgagagcga gctgttcggc cacgagaagg gggctttctc
     1321 cggcgccatc gccagtcgca agggccgctt cgagcttgcc gatggcggca cgctgtttct
     1381 cgatgaggtc ggcgagctgt cgctgccggc gcaggcgaag cttctgcgcg tgctgcagga
     1441 gcgcgagttc gagcgcgtcg gcggcagccg cccgatccgc gtcgacgtgc gcgtggtcgc
     1501 cgccaccaac cgcgacctgg cgcagatggt cgaggccggg ttgttccggg ccgatctgta
     1561 ctaccggctg aacgtcttcc cggtgcgcgt gccgccgctg cgcgagcggc ccgaggacat
     1621 cccgctgctg gtcacgcaca cgctcgccgg cctcgcgcgc cgcctcggca agccgctgca
     1681 cggcgtgagt cccgccgcgc gcgagcgcct catcgcctgg agctggccgg gcaacgtgcg
     1741 cgagctgcag aacgtcatcg aacgcgcggc gatcctcgcc agcggcgaca ccatcgaggt
     1801 cgaggatctc gggctgatgc cggtatcacc cggcgtcgcg ctcagcccgg tgacggggct
     1861 ggcggcgcag ccggtgccgg tggaggccgc cgccgcggca cgcccgctgc cgatggcgcc
     1921 ggcggcggag gaaggcgcca acctcgaagc cgtcgagcgc cgccacatcg agcaggtgct
     1981 caccggctgc aacggcgtca tcgaagggcc gaagggcgcg gcggcggcac tggggctgca
     2041 cccgagcacc ctgcggtcgc ggatgcagaa gctcggcatg cgcggcaggc gcgagtgatg
     2101 cccgctgccg cgctgtcctg cccatcggcc cggccggcgc gccggtggca tccgcctgcg
     2161 ggcggggagc atgcggatgt tccgccggcc atcctgccgg ctcgcgggcg gtggaatctt
     2221 tcgtgtccgt tgcggcccca gttcaccgac ccgcctgtgc agcctgagga ttcagcaacc
     2281 gtgaagattt accagcaact gatggccagc ctgttcgcga tgatcttcca ggggctggcg
     2341 ttcgcgctga tcctggtcgc cggctggcag ttcgccggcg cctggcgcga gggcagcgag
     2401 gaggtgctga gcatcgtcgt cagcggcatc aacacgctgg tgatctcgct ggcgatgtac
     2461 gaactcggcg tcggcgtcgg caaggagtac gaccccgaca actcgccgga caacgcctac
     2521 cagaccgtgc gccgcacgat cagccgcttc gtcggcacgg tctgcagcgc gctggtgctc
     2581 gaaggcctga tcatggcgat caagtacagc cagctcgatc tggccggcaa cctcgtctat
     2641 ccggtcgcgc tgctgatctc ggccgcggtg ctgctgatcg cgctcggcgt gttcctgcgc
     2701 ctgaccgcgc cgccggcggt gcagtcggag ccgcgctgat gccggcgtcc gcgcacgacc
     2761 gg
//
LOCUS       DAOQJH010000394         2762 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_25492_length_2762_cov_3.493905, whole genome shotgun sequence.
ACCESSION   DAOQJH010000394 DAOQJH010000000
VERSION     DAOQJH010000394.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2762)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2762)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2762
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25492_length_2762_cov_3.493905"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..599
                     /gene="gspJ"
                     /locus_tag="PLQ18_09250"
     CDS             <1..599
                     /gene="gspJ"
                     /locus_tag="PLQ18_09250"
                     /inference="COORDINATES: protein motif:HMM:TIGR01711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HNE01880.1"
                     /translation="GYTLLELLVALAVFAVVITAAYAGVVQFLRTAQRLEAEARRDAE
                     IQFAVDLFAQELAQAVARPVRDALGRREAALQLQGAQLLFTRTGWPAPPGQARTDLRR
                     VLYRHDHGQWLRGEQAAPDRIDAAPPSTSVLLGGLDAWHLRCLTRDGQWLEHWPPRGS
                     DEASAQLPLAIEAVFEFTDRGSVRRLLTLVSTWPGPGS"
     gene            596..1534
                     /gene="gspK"
                     /locus_tag="PLQ18_09255"
     CDS             596..1534
                     /gene="gspK"
                     /locus_tag="PLQ18_09255"
                     /inference="COORDINATES: protein motif:HMM:NF037980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HNE01881.1"
                     /translation="MSAPRQRGIALLIVLIALLLVAGIATRLAWVGAQSLRRAGTAQH
                     LAEARLRGLSAEAWARSLLAQDGRTTTTDHPGEAWATLPPVLPFEGGELRGRIVDLGG
                     RFNLNTLLNADGSVNEEALALFQGLLAALDIDPQRSAAVLDWLDADAEPRPGGAEDAQ
                     YALLARPRATGNRPFVDFGELGAVQGFDAAMLARLAPHVTALPIATPINVNTASGLLL
                     GLLLGYGRGSAQAALQLEQARPLPGYASEQAFLAQRIAVAPRYPLALNSRCFRLEVQI
                     DLAGVRVMQRSLLIRPDEAGRQAPLVLWRSFDGMAP"
     gene            1531..2700
                     /gene="gspL"
                     /locus_tag="PLQ18_09260"
     CDS             1531..2700
                     /gene="gspL"
                     /locus_tag="PLQ18_09260"
                     /inference="COORDINATES: protein motif:HMM:TIGR01709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HNE01882.1"
                     /translation="MKPAGTVIVRLRAAGLFEWQDGGGVHAGDAGAVAAALRRRRFVL
                     AIDTNELLLTEVPRLSRQRRLLAQALPAALEEMLAEDIDELHFATGPIEERLAVAVLR
                     RSRLIELLDAGWAAGLQPQAVLADVLLLPWRDGHWTLLHEDGQILLRCGRFRGYALSP
                     DELPALLELLQAEIGTGAHERPRLLLHGDALPPATIPPDWQASPQGKAGFWQICTDVV
                     RDRLDLSAGLQLRPRAEPRNYWPVALLLGLWAGVHLYADWSGNQALAREEAVLQGRVG
                     DLFRSLAPDIRRMVQPRVQLEQRLRERERQGGQETGLLGLLGALAPLLEAGDAQLMAL
                     SYREGSLDCTLSGGSLDQLENLRRGLDRAGRFSTQLQSAQRDGRVEGRLTIRLKP"
BASE COUNT          356 a          991 c         1016 g          399 t
ORIGIN      
        1 cgggctacac gctgctggaa ctgctcgtgg cactggccgt gttcgccgtg gtcatcaccg
       61 ccgcctatgc cggggtcgtg cagttcctgc gcaccgcgca gcgcctcgaa gccgaggccc
      121 ggcgcgatgc cgaaatccag tttgccgtcg atctgttcgc ccaggagctg gcccaggccg
      181 tggcccgccc cgtccgcgat gccctggggc ggcgcgaggc ggcgctgcag ctgcaggggg
      241 cgcagttgct gttcacgcgc acgggctggc cggctccgcc cggtcaggcg cgcaccgacc
      301 tgcgtcgcgt gctctatcgc cacgatcacg ggcagtggct gcgcggggaa caggctgcgc
      361 ccgatcgcat cgatgctgcg ccgccatcga cgagcgtgct gctcggcggc ctcgatgcct
      421 ggcatctgcg ctgtctgacg cgcgatggcc agtggctgga gcactggccg ccccgcggca
      481 gcgatgaagc ctcggcgcaa ctgccgctcg cgatcgaggc ggtgttcgag ttcaccgacc
      541 ggggcagcgt ccgccggctg ctcacgctgg tatcgacctg gccggggccc ggatcatgag
      601 tgcgccacgc cagcgcggca tcgcgctgct gatcgtgctg atcgcgctgc tgctggtcgc
      661 ggggatcgcc acgcggctgg cctgggtcgg tgcccagagc ctgcgccggg ccggcacggc
      721 ccagcatctg gccgaagccc ggctgcgcgg cctgagcgcc gaggcctggg cgcgcagcct
      781 gctcgcgcag gacggtcgca ccaccaccac cgatcacccg ggcgaggcct gggcaacgct
      841 gccgccggtg ctgccgttcg aaggcggcga gctgcgcgga cgcatcgtcg atctcggcgg
      901 gcggttcaac ctcaacacgc tgctcaatgc cgacggctcc gtgaacgagg aggcactggc
      961 actgttccag gggctgctcg cggcgctcga catcgatccg cagcgcagcg cagccgtgct
     1021 cgactggctc gacgccgacg cagaaccccg ccccggcggt gccgaggatg cccagtacgc
     1081 cctgctcgcc cggccgcgcg cgaccggcaa ccggccgttc gtcgactttg gcgaactggg
     1141 cgcggtgcag ggcttcgatg ccgcgatgct ggcgcgtctg gccccgcacg tgacggcgct
     1201 gccgatcgcg acgccgatca acgtcaacac ggccagcggc ctgctgctcg gcctgctgct
     1261 cggctacggt cggggcagcg cgcaggcggc cctgcagctg gaacaggccc ggccgctgcc
     1321 gggctatgcg agcgagcagg cgtttctggc gcagcggatc gctgtcgcgc cccgctatcc
     1381 gctggccctc aacagccgct gcttccggct ggaggtgcag atcgacctgg ccggggtgcg
     1441 cgtgatgcag cgcagcctgc tgatccgccc cgatgaagcc ggcagacagg ccccgctcgt
     1501 gctctggcgc agcttcgacg gcatggcccc atgaagccgg ccggcaccgt gatcgtccgg
     1561 ctgcgcgccg ccggcctttt tgaatggcag gacggcggcg gcgtgcatgc cggcgatgcc
     1621 ggggccgtcg ccgccgccct gcggcggcgg cgcttcgtgc tcgccatcga cacgaacgag
     1681 ctgctgctga ccgaagtccc gcggctgagc cgccagcggc gcctgctggc gcaggccctg
     1741 ccggcggcac tcgaggagat gctcgcggag gacatcgatg agctgcactt cgccaccgga
     1801 ccgatcgagg agcgtctggc cgtggccgtg ctgcgccgca gccggctgat cgagctgctc
     1861 gatgccgggt gggcggcggg gctgcagccg caggcggtgc tggccgacgt gctgctgctg
     1921 ccctggcggg acgggcactg gacgctgctc catgaggacg ggcagatcct gctgcgctgc
     1981 gggcgctttc gcggctatgc actcagcccc gacgaattgc cggcgctgct ggaactgctg
     2041 caggccgaaa tcggcaccgg cgcgcacgag cggccgcggc tgctgctgca cggcgatgcc
     2101 ctgccgcccg cgacgattcc gccggactgg caggcctcgc cgcagggcaa ggcaggcttc
     2161 tggcagatct gcaccgacgt ggtacgggac cgcctcgacc tgagcgccgg cctgcagctc
     2221 cggccccggg cggaaccgcg caactactgg cccgtggccc tgctgctcgg cctgtgggcc
     2281 ggggtgcatc tgtatgccga ctggagcggg aaccaggcgc tggcccgcga ggaagccgtg
     2341 ctgcagggcc gcgtcgggga cctgttccgc agcctggcgc ccgatatccg gcgcatggtc
     2401 cagccgcgcg tgcagctcga acagcgcctg cgcgagcgtg aacgtcaggg cggacaggaa
     2461 accggcctgc tcggcctgct cggtgcgctc gccccgctgc tcgaagccgg cgatgcgcag
     2521 ctgatggccc tcagctatcg cgagggcagc ctcgactgca cgctgagcgg cggcagtctc
     2581 gatcagctgg aaaacctgcg ccgcggcctc gaccgggcgg gccggttcag cacgcagctg
     2641 cagtcggcgc agcgcgacgg cagggtcgaa ggacgcctga cgatccggct gaagccatga
     2701 acgctttctg gtcacggctt tcgctgcgcg aacgctggct gctggcgggg acgggatgca
     2761 tc
//
LOCUS       DAOQJH010000395         2760 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_25527_length_2760_cov_3.780407, whole genome shotgun sequence.
ACCESSION   DAOQJH010000395 DAOQJH010000000
VERSION     DAOQJH010000395.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2760)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2760)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2760
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25527_length_2760_cov_3.780407"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..437
                     /locus_tag="PLQ18_09265"
     CDS             <1..437
                     /locus_tag="PLQ18_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HNE01883.1"
                     /translation="RLAGQIGLEPGCRNPFKSILARVIEVLYALGEARRLIDGYRVPE
                     APAIDCPPRAGTGYGATEAPRGLCYHRYTLAADGVITDAKIVAPTSVNQRQIEADLHE
                     YAASRLDQSDERLRHGCERLIRSYDPCISCSTHFLTLNIENG"
     gene            complement(624..929)
                     /locus_tag="PLQ18_09270"
     CDS             complement(624..929)
                     /locus_tag="PLQ18_09270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE01884.1"
                     /translation="MEALDLNKTDLRRTTSYQLHRLALLLVDRLDREQQRANLVAEVR
                     KWRLRRRMRMIVSELLSRRSLDEVLSMAAASASDAHPQERSGELSRRYVEMIRSFHA"
     gene            1788..2048
                     /locus_tag="PLQ18_09275"
     CDS             1788..2048
                     /locus_tag="PLQ18_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018237578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2282 domain-containing protein"
                     /protein_id="HNE01885.1"
                     /translation="MKTQTLLQSALATAIALGAGFGSIAHAADAPAEKCYGVVKAGKN
                     DCQTNTHACAGQAKADSQGDSWVYLPKGACEKITGGSLTKKA"
     gene            2057..>2760
                     /locus_tag="PLQ18_09280"
     CDS             2057..>2760
                     /locus_tag="PLQ18_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018422955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HNE01886.1"
                     /translation="MNDGAPVSGRQSAASMPAIAGIGLRGDHYEALLAGDFGDQAGPG
                     WVEVHSENYFGDGGWPLAVLDRVRAHWPVSLHGVGLGLGNADGLDRGHLARLQALVAR
                     VEPGWVSEHLCWTAAGGQHLNDLLPLPMSEEALALVCRHVQQVQEALGRPLLVENVSR
                     YLDFAASVIPEGEFLAELVARTGCGLLLDVNNLYVNHINHGDDPLAIVAALPAAAIGE
                     IHLAGHSRREVRGQALL"
BASE COUNT          478 a          940 c          907 g          435 t
ORIGIN      
        1 cccggctcgc cgggcagatc ggactggagc ccggctgccg caacccgttc aagtccatcc
       61 tcgcgcgtgt gatcgaggtg ctgtacgcac tcggcgaggc gcgccggctg atcgacggat
      121 accgcgtgcc cgaggctccg gccatcgact gcccgccgcg cgccggcacc ggctacggcg
      181 ccaccgaggc cccgcgcggc ctctgctatc accgctacac gctcgccgcc gacggcgtga
      241 tcaccgacgc gaagatcgtc gccccgacct cggtcaacca gcgccagatc gaggccgacc
      301 tgcacgagta cgccgccagc cgcctcgatc agtccgacga gcgcctgcgc cacggctgcg
      361 aacggctgat ccgcagctac gacccgtgca tctcctgctc gacgcacttc ctgacgctga
      421 acatcgaaaa cggttgagga aagccgctaa aaatataatt tttataccat catattgcgt
      481 gctgccactt ccgcagcagg aacaggtttc tgatgaccgg gcggagaccg gtgcgcatca
      541 ccggaagcga tctgacaggg cagcgctctg ccgaccgtag cgcgggatac ccgccggccc
      601 tctgcgggcc ggcagcgggg ggatcaggcg tggaagctgc ggatcatctc gacgtagcgg
      661 cgcgacagtt cgccgctgcg ttcctgcgga tgggcatcgg aagcgctcgc ggcggccatc
      721 gacagcactt cgtcgaggct gcggcggctc agcagttcgg acacgatcat gcgcatgcgc
      781 cggcgcagcc gccatttgcg cacctcggcc acgaggttgg cgcgctgctg ttcacgatcg
      841 agccggtcga ccagcagcag cgcgagccgg tgcaactggt aggacgtggt gcggcgcagg
      901 tcagtcttgt tcagatcgag ggcttccatg gctcatcggt cctccggcat ggggggtgag
      961 gaaatgtgaa taaatcttaa tgacactaga gtcattactc aacgcacgtc ctgtgcattg
     1021 cattgcagca tattcagcgg aatttacaaa tgtaaagtga tgccggatgc ggcgtaaggt
     1081 tgctgaccgc cgccggcacc ctcctgaaac attcatgcat cagtcgtgct aagtaccggt
     1141 gcaaagtgaa gaatctgagc agccacgccg tcggccggcc ctggccgtgt cgtggtctgg
     1201 ccgcaaggcg ggactgccgg aggcattcgg caggcgtgcc ggaagatgct gtttcatcgg
     1261 gttttcttac acttttccgg caaggggcgt gagcggcggc caaggctcac gcacgcgctg
     1321 cgccgctccg tcaccgccag cgcttgcccg gacacccttg aaaaaaaatc atccgaagcc
     1381 ccccgcaccg gaatccgggc tgcagggcgc ctgcacgccg gatgatccgg aatgcaccgc
     1441 ctgaaagcgt ggccatccgc tgctgcacgc ggagcgtgct tcccggacgg caggggcacc
     1501 cgggggcagg tggctgcgcg tgaagtcagc cgcgatcggg cgcgtcagcc cgcgaaatcc
     1561 cgtggccgtg gcgggacgga gcgccggacg cgaacccggc aatccgccgg ctcctgatca
     1621 aagatgaaca taaacattga cttgtctgga tgccttcccg gtgcatccgg ctggcacggc
     1681 gactgcgtaa ggggaatcgt ccgcagcagc acgaaaaggc caacacggcc gctgcacgga
     1741 tcctctccca gaccctgaac cgttttcccc catccggaga ttgcgccatg aagacccaga
     1801 ccctgctgca gtccgcgctc gccaccgcca tcgccctcgg cgccggtttc ggcagcatcg
     1861 cccatgccgc cgacgcgccg gccgaaaagt gctacggcgt ggtcaaggcc ggcaagaacg
     1921 actgccagac caacacccac gcctgcgccg gccaggccaa ggccgactcg cagggcgact
     1981 cctgggttta tctgcccaag ggcgcctgcg agaagatcac cggcggcagc ctgaccaaga
     2041 aggcgtgagc gacgccatga atgacggcgc gccggtatcc ggtcgccaga gtgctgcgtc
     2101 gatgccggca atcgccggca tcggcctgcg cggcgatcac tacgaagccc tgctggccgg
     2161 cgacttcggc gatcaggccg gtcccggctg ggtcgaggtc cacagcgaga actacttcgg
     2221 cgacggcggc tggccgctcg ccgtgctcga tcgcgtgcgc gcgcactggc ccgtcagcct
     2281 gcacggcgtc ggtctgggcc tcggcaacgc cgatggtctc gatcgcgggc atctggcgcg
     2341 gctgcaggcg ctggtcgcac gcgtcgagcc ggggtgggtg tccgaacatc tctgctggac
     2401 cgccgccggg ggccagcacc tgaacgacct gctgccgctg ccgatgagcg aggaagccct
     2461 cgccctcgtc tgccgtcacg tgcagcaggt acaggaagcc ctgggccggc cgctgctggt
     2521 cgagaacgtc tcgcgctatc tcgacttcgc cgcctcggtg atccccgagg gcgagtttct
     2581 cgccgagctg gtcgcgcgca ccggctgcgg gctgctgctc gacgtgaaca acctgtacgt
     2641 gaaccacatc aaccacggcg acgatccgct ggcgatcgtc gcggcgctgc cggcggcggc
     2701 gatcggcgag attcacctgg ccggtcacag ccggcgcgag gtgcgcggtc aggcgctgct
//
LOCUS       DAOQJH010000396         2756 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_25589_length_2756_cov_4.305442, whole genome shotgun sequence.
ACCESSION   DAOQJH010000396 DAOQJH010000000
VERSION     DAOQJH010000396.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2756)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2756)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2756
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25589_length_2756_cov_4.305442"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..488
                     /locus_tag="PLQ18_09285"
     CDS             <1..488
                     /locus_tag="PLQ18_09285"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010959391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HNE01887.1"
                     /translation="IARGERMDYALQKAVELGVQAIRPVFTARSNVQLDADRLDKRLA
                     HWRAVIVAACEQCGRNRLPVLAAPLRIEALLAETAAGDGGLRLVLDPLAQHGLRALAP
                     PAGLVTLLVGPEGGLAEAELAAATAAGWQGVRLGPRVLRTETAGVAALAALAALWGDL
                     G"
     gene            607..1065
                     /locus_tag="PLQ18_09290"
     CDS             607..1065
                     /locus_tag="PLQ18_09290"
                     /inference="COORDINATES: protein motif:HMM:NF019032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1499 domain-containing protein"
                     /protein_id="HNE01888.1"
                     /translation="MQILTTLIALAGCLAIGALVLNGVRLGEPPGMGRRLLTYLNNHV
                     AETRRGHDFPELELRCYRMSPAQLYDRVEQALRVLNWEIVEASPQAQSLHATVTSQLL
                     RFVDDVHIELRVAACGTELHVRSQSRIGRGDFGANARHVMELHLTLDRLL"
     gene            complement(1070..1555)
                     /locus_tag="PLQ18_09295"
     CDS             complement(1070..1555)
                     /locus_tag="PLQ18_09295"
                     /inference="COORDINATES: protein motif:HMM:NF023338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3429 domain-containing protein"
                     /protein_id="HNE01889.1"
                     /translation="MTPSAGPSAGPSPSAQDPARASETAQWLCYVGLLPFIGATATLA
                     LGGEAWHDTALQALIGYGAVILGFLGAVHWGLALREQPLDHRRHYFGWGVLPALAGWL
                     ALMIPPRTGLGLLFAGYALQYAADRHATVQDALPLWYERLRLKLSQVVLLSLAIAWAL
                     A"
     gene            1985..2725
                     /locus_tag="PLQ18_09300"
     CDS             1985..2725
                     /locus_tag="PLQ18_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020679915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HNE01890.1"
                     /translation="MSSTHSGLRACALACTLLLGACAHQPQLAPLDPEAIDRLPAYNR
                     WMFGVNEKLDEYLLKPVAQGYQAVTPQFLDDGITNIFGNLADLPIGINNLLQFKFREA
                     AQDFTRIVFNSTFGLLGFFDVASYFGIPKHDEDFGQTLAHWGVGEGSYFVLPLLGPST
                     LRDTGGLAVDAFNIDPVWEINRIPVRNSLVGLRIVDKRADLLRSERVTEGALLDRYTQ
                     IRDAWLQRRRSLIYDGNAPLEPRRLDVD"
BASE COUNT          419 a          987 c          966 g          384 t
ORIGIN      
        1 gcatcgcccg tggcgagcgc atggattacg cgctgcagaa ggccgtcgaa ctcggcgtgc
       61 aggcgatccg gccggtgttc acggcgcgct cgaacgtgca gctcgacgcc gatcgcctcg
      121 acaagcgcct cgcacactgg cgcgccgtca tcgtcgccgc ctgcgagcag tgcggccgca
      181 accgcctgcc ggtgctggcc gcgccgctgc gcatcgaggc gctgctcgcc gaaaccgccg
      241 ccggtgacgg cggcctgcgg ctggtgctcg acccgctggc acagcacggc ctgcgtgcgc
      301 tcgcgccgcc tgcgggcctg gtgacgctgc tggtcgggcc cgaaggcggg ctggccgaag
      361 cggagctggc ggcggccacg gcggccggct ggcagggcgt gcggctcggt ccgcgcgtgc
      421 tgcgcaccga gactgccggc gtcgcggcac tggccgcact ggcagcgctg tggggcgatc
      481 tgggctaggt tcgatcgcca gcaccgggaa ccccgcgcag cgctgcagac tccgtccccc
      541 gggctgctcg ccacaaccgc ggcctcccgc cacacgggct cgaaccacat gaggatgcga
      601 agaacgatgc agatcctgac gacgctgatc gcgctggccg gctgtctggc catcggggcg
      661 ctggtgctga acggggtgcg cctcggcgaa ccgcccggca tgggccggcg cctgctgacc
      721 tacctgaaca accacgtcgc cgagacccgg cgcggccacg atttcccgga gctggaactg
      781 cgctgctacc ggatgtcgcc ggcgcagctg tacgaccgcg tcgaacaggc gctgcgggtc
      841 ctgaactggg agatcgtcga ggccagcccg caggcgcagt cactgcacgc gaccgtgacc
      901 agccagctgc tgcgcttcgt cgatgacgtg cacatcgaac tgcgcgtcgc cgcctgcggc
      961 accgagctgc acgtgcgctc gcaatcgcgc atcggccgcg gcgacttcgg cgccaacgcc
     1021 cgccacgtca tggaactgca cctgacgctg gaccggctgc tgtagcgcgt caggccagcg
     1081 cccaggcgat cgccagcgac agcagcacga cctgcgacag cttcagccgc agccgctcgt
     1141 accagagcgg cagcgcgtcc tgcacggtcg cgtgacgatc ggcggcgtac tgcagcgcgt
     1201 agccggcgaa cagcaggccg aggccggtgc gcggcgggat catcagcgcc agccagccgg
     1261 ccagcgccgg cagcacgccc cagccgaagt agtgccggcg gtgatcgagc ggctgctcgc
     1321 gcagcgccag cccccagtgc acggccccga ggaaaccgag gatcaccgcg ccgtagccga
     1381 tcagcgcctg cagcgcggtg tcgtgccagg cctcgccgcc aagggcgagc gtggcggttg
     1441 cgccgatgaa gggcagcagg ccgacatagc agagccactg cgccgtttcg gacgcgcggg
     1501 ccggatcctg cgcgctgggg gatggaccgg cggatggacc ggcagatgga gtcatgatcg
     1561 cgggctcctt cagagcagct tttcgatcgc tgtgagcagt gtggggtctt gcgggccggt
     1621 ctgcgcagga aaacttgcaa caagccgtcc cgagcggtcg atccggcact tccggaagct
     1681 gcggcgcggc gcctgtccgg cggctgcgct gagcgcgcag cagaacggat gccgggcgtg
     1741 gccggccgcg gacggggttc cggcagcgac cggcggcacg agcgccagcc tcaggacgaa
     1801 tcaggaacgg cgcgagcgca aacggcatgg gggctccccg gaacgtcagg acgggcaggt
     1861 ctgatttgga acgcgtccgt cgccggcggt tcgcctgcct gccgatcgct gcatggccgg
     1921 gcgaggcggc atgcaggtat catcggccgc atcgttcctg tcttcagccc gaggatccgg
     1981 ggtcatgtca tccacccact ccggcctgcg cgcctgcgcg ctcgcctgca cgctgctgct
     2041 cggcgcctgc gcccaccagc cccagcttgc gccgctcgat cccgaagcga tcgaccggct
     2101 gccggcgtac aaccgctgga tgttcggcgt caacgagaag ctggacgaat atctgctcaa
     2161 gccggtcgcg cagggctatc aggcggtgac accgcagttc ctcgacgacg gcatcaccaa
     2221 catcttcggc aacctcgccg acctgccgat cggcatcaac aacctgctgc agttcaagtt
     2281 ccgggaagcc gcgcaggact tcacgcgcat cgtcttcaac tcgaccttcg gcctgctcgg
     2341 cttcttcgac gtggcgagct acttcggcat cccgaagcac gacgaggact tcggccagac
     2401 gctcgcgcac tggggcgtcg gcgagggcag ctacttcgtg ctgccgctgc tcggcccgag
     2461 cacgctgcgc gacaccggcg gcctggcggt cgatgcgttc aacatcgatc cggtctggga
     2521 gatcaaccgc atcccggtgc gcaactcgct ggtggggctg cgcatcgtcg acaagcgtgc
     2581 cgacctgctg cgcagcgagc gcgtcaccga aggcgcgctg ctcgatcgct acacgcagat
     2641 ccgcgatgcc tggctgcagc gccgccgcag cctgatctac gacggcaacg ctccgctgga
     2701 gccgcggcgc ctcgacgtcg actgagcgag cgctgcagcg cgcgatgccc cgcctg
//
LOCUS       DAOQJH010000397         2756 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_25598_length_2756_cov_2.819326, whole genome shotgun sequence.
ACCESSION   DAOQJH010000397 DAOQJH010000000
VERSION     DAOQJH010000397.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2756)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2756)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2756
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25598_length_2756_cov_2.819326"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            148..645
                     /locus_tag="PLQ18_09305"
     CDS             148..645
                     /locus_tag="PLQ18_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018260232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNE01891.1"
                     /translation="MTGPVPLPARRPETPADADFLSGLFRAHRAEAFAVPGLPPADLD
                     ALLRLQVQAQVAHYRRQWPQARAEIVLDAGAAPIGRLLSARSVRELRLIDIALLPACC
                     GCGLGTRLIAALIDEAGAAGLALTAHVARDNPARRLYARLGFREHDDGGPYLAIRHAL
                     PGREA"
     gene            complement(659..2497)
                     /locus_tag="PLQ18_09310"
     CDS             complement(659..2497)
                     /locus_tag="PLQ18_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018311055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HNE01892.1"
                     /translation="MSEPRYSVGIDLGTTHCVLAAADLSLTDGETVALALEPVAQLTA
                     PGAVEERTLLPSFLYLPHPDELAPADLALPWDTPEGFLVGELARSLGAKTPLRLVASA
                     KSWLCHPGIDRRGPVLPVEAPAEVARISPFDATVRYLEHLADAWDARHPDAPLDAQAL
                     TITVPASFDPAARELSAEAAQAAGLEDFILLEEPQAALYSWIEATAGGWRKQARVGDV
                     ILVADVGGGTADFSLIAVGERDGGLDFTRIAVGDHILLGGDNMDLALAHVLRAKLAAA
                     GTQLEPWQVQGLVHGCRAAKEQILGDAAADAVPVVVPSRGSRLIGGTLRTELTRVEVE
                     ATLVEGFFPRVGAEARPLARARTGLTRLGLPYAQDAGVTRHLAAFLARQLDAAPGCGI
                     ELPAGASFLHPTAVLFNGGVFKAAALEARVLDVLNAWLAAEGAPPARRLPGTQLDHAV
                     ARGAAYYGCVRRGRGVRIRGGTARSYYVGIESSMPAVPGIEPPVEALCVAPFGMEEGT
                     AAELPAQEVGLVVGEPVRFRFFGSPVRRDDRVGDVLEVWGGEELEELPEIEATLPAEG
                     RTPGEVVPVRLHAAVTELGTLRLEAVSRDASERWKVELEVRQADEA"
     gene            complement(2577..>2756)
                     /locus_tag="PLQ18_09315"
     CDS             complement(2577..>2756)
                     /locus_tag="PLQ18_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004315499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2760 domain-containing protein"
                     /protein_id="HNE01893.1"
                     /translation="LTLPAGFDAGAVRLTGRVLGQPPFTGTLVHCGWRASAVKLPKLA
                     EGHDVHVVAPAEVEL"
BASE COUNT          357 a         1018 c         1047 g          334 t
ORIGIN      
        1 caatgcgctg tacgcggcga cggccgatgc gacgatgtcc gccgcggcca tctcgacggc
       61 cggcagcagc cagccgcatc cgaaccgtca gccgtaccag tgcctgtact ggatcatcgc
      121 gaccgaaggc gtgtttccgt cgcgcgcatg acgggccccg tgccgctgcc ggcgcgccgg
      181 ccggaaaccc cggccgatgc ggacttcctg agcgggctgt tccgcgcgca ccgggcggag
      241 gcattcgccg tgcccggcct gccgccggcg gacctcgacg cattgctgcg cctgcaggtg
      301 caggcgcagg tcgcgcacta ccgccggcaa tggccgcagg cgcgcgccga gatcgtgctc
      361 gacgccggcg cggccccgat cgggcggctg ctgagcgccc gcagcgtgcg ggaactgcgc
      421 ctgatcgaca tcgcgctgct gccggcctgc tgcgggtgcg ggctcggcac gcggctgatc
      481 gcggcgctga tcgacgaggc cggcgccgcc ggcctggcgc tcaccgcgca cgtcgcgcgc
      541 gacaacccgg cgcgacggct gtatgcgcgg ctcggtttcc gcgagcacga cgacggcggg
      601 ccgtacctcg cgatccgcca cgccttgccc ggccgggagg cctagaggat gtctgcgttc
      661 aggcctcgtc ggcctgacgc acctccagct cgaccttcca gcgctcgctc gcatcgcgtg
      721 acaccgcttc gaggcgcagc gtgccgagtt cggtgaccgc cgcgtgcagg cgcaccggca
      781 cgacctcgcc gggcgtgcgg ccctcggccg gcagcgtcgc ctcgatctcg ggcagttctt
      841 cgagctcctc gccgccccag acctccagca cgtcgccgac gcggtcatcg cggcgcaccg
      901 gcgagccgaa gaagcggaag cgcaccggct cgccgaccac gaggccgacc tcctgcgccg
      961 gcagctcggc cgcggtgcct tcctccatgc cgaagggcgc gacgcacagc gcctcgaccg
     1021 gcggctcgat gccgggcacg gccggcatcg agctttcgat gccgacgtaa tagctgcgcg
     1081 ccgtgccgcc gcggatgcgc acgccgcggc cgcggcgcac gcagccgtaa taggccgcgc
     1141 cgcgtgcgac cgcgtgatcg agctgcgtgc caggcaggcg ccgggccggc ggcgcgccct
     1201 cggcggcgag ccaggcgttc agcacgtcga gcacgcgcgc ttcgagcgcg gccgccttga
     1261 acacgccgcc gttgaacagc accgccgtcg ggtgcaggaa gctcgcgccg gccggcagct
     1321 cgatgccgca gccgggtgcc gcgtcgagct ggcgggcgag gaaggccgcg aggtggcggg
     1381 tgacgccggc gtcctgcgca tacggcaggc cgagccgggt caggccggtg cgcgcgcgcg
     1441 ccagcggccg ggcctcggcg ccgacgcgcg ggaagaagcc ctcgaccagc gtcgcctcga
     1501 cctcgacgcg cgtcagctcg gtgcgcagcg tgccgccgat cagccgcgag ccgcggctcg
     1561 gcaccacgac cggcaccgca tcggccgcgg catcgccgag gatctgctcc ttggcggcgc
     1621 gacagccgtg cacgaggccc tgcacctgcc agggttcgag ctgcgtgccg gcggcggcga
     1681 gcttggcgcg cagcacgtgt gcgagcgcca ggtccatgtt gtcgccgccg agcaggatgt
     1741 gatcgccgac cgcgatgcgc gtgaagtcga gcccgccgtc gcgctcgccg accgcgatca
     1801 gcgagaagtc cgccgtgccg ccgccgacgt cggcgacgag gatcacgtcg ccgacgcgcg
     1861 cctgcttgcg ccagccgccg gcggtcgcct cgatccacga atacagcgcc gcctgcggtt
     1921 cttcgagcag gatgaagtct tcgaggccgg cggcctgcgc ggcctcggcg ctcagctcgc
     1981 gcgcggccgg gtcgaacgag gccggcaccg tgatcgtcag cgcctgcgca tcgagcggcg
     2041 cgtcggggtg gcgcgcgtcc caggcatcgg cgaggtgttc gaggtagcgc accgtggcat
     2101 cgaagggcga gatgcgcgcg acctcggccg gcgcctcgac cggcagcacc gggccgcggc
     2161 ggtcgatgcc ggggtggcag agccagctct tggcgctcgc gaccaggcgc agcggcgtct
     2221 tggcgccgag gctgcgcgcg agctcgccga ccaggaagcc ctcgggcgtg tcccagggca
     2281 gcgccagatc ggccggtgcc agctcatccg gatgcggcag gtacaggaag gacggcagca
     2341 gcgtgcgctc ctcgacggcg ccgggcgcgg tgagctgcgc aaccggttcg agggcgagcg
     2401 cgacggtctc gccgtcggtc aggctcaggt cggcggcggc gagcacgcag tgcgtggtgc
     2461 cgagatcgat gccgacggaa taacggggtt cgctcatggc ggtctccgga gagggacgga
     2521 agcggtggcc gcggggggcg ccgggaggcg ctccggtgaa ggcttcggcg ccgggcctac
     2581 agctcgacct cggccggtgc gacgacgtgc acgtcgtggc cctcggcgag cttcggcagc
     2641 ttcacggcac tcgcgcgcca gccgcagtgc accagcgtgc cggtgaacgg cggctggccg
     2701 agcacgcggc cggtcaggcg cacggcaccg gcatcgaagc cggccggcag cgtcag
//
LOCUS       DAOQJH010000398         2751 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_25674_length_2751_cov_3.516691, whole genome shotgun sequence.
ACCESSION   DAOQJH010000398 DAOQJH010000000
VERSION     DAOQJH010000398.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2751)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2751)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2751
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25674_length_2751_cov_3.516691"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..677
                     /locus_tag="PLQ18_09320"
     CDS             <1..677
                     /locus_tag="PLQ18_09320"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNE01894.1"
                     /translation="ATAAAGAPAGAAAETPAMAPTGTAAPLPELTVDTFRFLVERLAQ
                     TEHKRVQLLASGLDTLAVSGAEQITALREVLVQLLRNAVAHGIETPEERHLAGKTDVG
                     TIRLQARADASAGIELICHDDGRGLDPGRIRAAALQSGRWSEDELDAMGTAGLRALLF
                     EPGFTTAAEATLAAGRGVGMDVIKHRVEALGGTLSVASEPGRYCAWRITLPGLPGLGE
                     LSFETT"
     gene            723..1103
                     /locus_tag="PLQ18_09325"
     CDS             723..1103
                     /locus_tag="PLQ18_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007864515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE01895.1"
                     /translation="MPEPKPLTLLIVDDSHIIRRRIERSVAIDGLDIVGTAADGDEAV
                     RKFRKLLPDAVTMDLTMPGMDGLQTISAVLAIRPDTRILVVSALADRSTAIEAIRRGA
                     RGFLYKPFDDAQLNEALTELLELH"
     gene            1103..1558
                     /locus_tag="PLQ18_09330"
     CDS             1103..1558
                     /locus_tag="PLQ18_09330"
                     /inference="COORDINATES: protein motif:HMM:NF025070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HNE01896.1"
                     /translation="MLNETQLKVFVDHATHYFSAFAQEPAIVRVPYLISHDESLVYDY
                     TGVIGVSGAFRGCVYFTAPARMMQYLLMTQGETEHSQEHCLDVVGEIATTLAGNARRE
                     LGSDFMISVPIRIAGKPEQLTLARSARSYAIPIQWRVYSAALVVSLEDG"
     gene            1647..2273
                     /locus_tag="PLQ18_09335"
     CDS             1647..2273
                     /locus_tag="PLQ18_09335"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003139074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HNE01897.1"
                     /translation="MSSVYALKPRFQAWLRPAVARLAAAGITANQVTLAAAGASMLLG
                     VALYAAPNVPALWLLLPLFLFVRMALNAIDGMLAREHGQQSALGACLNELGDVFADAL
                     LYLPFARLPACPTRWVVAFVLLAVICEMAGVLMQTLGASRRYDGPMGKSDRAVACGAL
                     GLALGLGATPGPWLEVAFVIFSALGLWTLVRRVTRGLAERGAAPAGRA"
     gene            complement(2305..>2751)
                     /locus_tag="PLQ18_09340"
     CDS             complement(2305..>2751)
                     /locus_tag="PLQ18_09340"
                     /inference="COORDINATES: protein motif:HMM:NF023329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3421 domain-containing protein"
                     /protein_id="HNE01898.1"
                     /translation="GDDWKYRSLLETKLSLFEHFRGTGRTQNELEDRDGSKPDTGVLE
                     FVGPNQKLPSLPELAVVHLGGLTLRDSYLPSGVGVWVAASGGAVPFGAIVGGHEAAPG
                     NEPLYICRATHNGGVHPGKLRAAFKGCNIGWGGRETVVADYEVLVR"
BASE COUNT          384 a          994 c          961 g          412 t
ORIGIN      
        1 aagcgacggc cgctgccggg gcgccggccg gggccgcggc cgaaaccccg gcgatggccc
       61 cgaccggcac cgctgcgccg ctgccggagc tgaccgtcga caccttccgc ttcctggtcg
      121 agcgtctggc gcagaccgag cacaagcgcg tgcagctgct cgcgagcggc ctcgacacgc
      181 tcgcggtcag cggcgccgag cagatcacgg cgctgcgcga ggtgctggtg cagctgctgc
      241 gcaacgccgt cgcgcacggc atcgagaccc cggaggagcg ccacctcgcc ggcaagaccg
      301 atgtcggcac catccgcctg caggcacgcg ccgatgccag cgccggcatc gagctgatct
      361 gccacgacga cggccgcggc ctcgaccccg ggcgcatccg cgccgccgcg ctgcagagcg
      421 ggcgctggag cgaggacgag ctggacgcga tgggcacggc cgggctgcgc gcgctgctgt
      481 tcgagccagg cttcacgacc gccgccgaag ccaccctcgc cgccggccgc ggtgtcggca
      541 tggacgtgat caagcaccgc gtcgaggcgc tcggcggcac gctgtcggtg gcctcggaac
      601 cgggacgcta ctgcgcgtgg cgcatcacgc tgcccggcct gccgggactc ggcgaactca
      661 gcttcgagac gacctgatca tgccccctgc cctgcgacga cagactcatt gaggacagga
      721 ccatgccgga accgaagccg ctgacgctgc tgatcgtcga cgattcacac atcatccgcc
      781 gccgcatcga acgcagcgtc gccatcgacg gcctcgacat cgtcggcacc gccgccgatg
      841 gcgacgaggc cgtgcgcaag ttcaggaagc tgctgcccga cgccgtgacc atggatctga
      901 ccatgcccgg catggacggc ctgcagacga tctcggcggt gctggcgatc cggcccgaca
      961 cgcgcatcct ggtcgtctcg gcgctcgccg atcgctcgac cgcgatcgag gcgatccgcc
     1021 gcggcgcgcg cggcttcctc tacaagccct tcgatgacgc gcagctcaac gaggcgctga
     1081 cggaactgct ggaactgcac tgatgctgaa cgaaacccag ctcaaggtct tcgtcgacca
     1141 cgccacgcat tacttctcgg ccttcgcgca ggagccggcg atcgtgcgcg tgccctacct
     1201 gatcagccac gacgagtcgc tggtctacga ctacaccggc gtcatcgggg tttcgggcgc
     1261 gttccgcggc tgcgtgtact tcacggcccc ggcgaggatg atgcagtacc tgctgatgac
     1321 gcagggcgag accgagcact cgcaggagca ctgcctcgac gtggtcggcg agatcgccac
     1381 cacgctcgcc ggcaacgccc ggcgcgaact cggctccgac ttcatgatct cggtgccgat
     1441 ccgcatcgcc ggcaagcccg agcagctgac cctcgcccgc agcgcccgct cgtatgcgat
     1501 cccgatccag tggcgcgtct acagcgcggc gctggtcgtg agtctggagg acggctgagg
     1561 cgcgatgcga tccggctgct gatcctgtcg tgaggccggc cacctacgct gtggccgtct
     1621 cccccgccct cgcaggatcg tccccgatga gcagcgtcta tgccctgaaa ccccgcttcc
     1681 aggcctggct gcggccggcc gtggcccggc tggctgcggc cggcatcacg gcaaaccagg
     1741 tcacgctcgc cgccgccggc gcctcgatgc tgctcggcgt ggcgctgtac gcggcgccga
     1801 acgtgccggc gctgtggctg ctgctgccgc tgttcctgtt cgtgcgcatg gcgctgaacg
     1861 cgatcgacgg catgctggcg cgcgagcacg gccagcagtc ggcactcggc gcctgcctga
     1921 acgaactcgg cgatgtcttc gccgatgcgc tgctgtacct gccgttcgcg cggctgccgg
     1981 cctgcccgac gcgctgggtc gtggccttcg tgctgctggc ggtgatctgc gagatggccg
     2041 gcgtgctgat gcagacgctc ggtgccagcc gccgctacga cggcccgatg ggcaagagcg
     2101 atcgtgccgt cgcctgcggt gcgctcgggc tcgcgctcgg actcggggcg acgccggggc
     2161 cgtggctgga ggtggcgttc gtgatcttct cggcgctcgg gctctggacg ctggtgcgcc
     2221 gcgtcacgcg cggactggcc gagcgtggcg cggcgcccgc gggccgggcg tgaacgtgaa
     2281 cgtgaacgtg aacgtgaacg cgtattagcg caccagcacc tcgtagtcgg cgaccaccgt
     2341 ctccctgccg ccccagccga tgttgcagcc cttgaaggcc gcgcgcagct tgccggggtg
     2401 gacgccgccg ttatgcgtcg cgcggcagat gtacagcggt tcgttgccgg gcgcggcctc
     2461 gtggccgccg acgatggcgc cgaaaggcac cgctccgccg ctggccgcga cccagacgcc
     2521 gacgcccgag ggcaggtagc tgtcgcgcag cgtcaggccg ccgaggtgca cgacggcgag
     2581 ttcgggcagg gacggcagct tctggttcgg gccgacgaac tccagcacgc cggtgtcggg
     2641 cttgctgccg tcgcggtcct ccagttcgtt ctgcgtgcgg ccggtgccgc ggaagtgctc
     2701 gaacaggctg agcttggtct cgagcagcga gcggtacttc cagtcatcgc c
//
LOCUS       DAOQJH010000399         2749 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_25717_length_2749_cov_3.603935, whole genome shotgun sequence.
ACCESSION   DAOQJH010000399 DAOQJH010000000
VERSION     DAOQJH010000399.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2749
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25717_length_2749_cov_3.603935"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(275..538)
                     /locus_tag="PLQ18_09345"
     CDS             complement(275..538)
                     /locus_tag="PLQ18_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016390382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE01899.1"
                     /translation="MLSYVDCVGFAGLTEDEIEAIAEHEHVPEIVAAEFGCCLCETTE
                     GVPLMRRILLDDIRHAEAHGAVLHALHLRAVLAKFEAGHEVLH"
     gene            757..1197
                     /locus_tag="PLQ18_09350"
     CDS             757..1197
                     /locus_tag="PLQ18_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006156586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNE01900.1"
                     /translation="MNTGDKTAGTTYTISQLAREFDVTTRAIRFYEDQGLLIPERVGR
                     RRIYARRDRTRLKLILRGKRLGMTLGETRELFDLFDSSRDEARQLERFLRILGERRDI
                     LVQQQRDIEAVLHEINAAAAECERLLAEHAEPSPQDPAGPTVPA"
     gene            1376..2554
                     /locus_tag="PLQ18_09355"
     CDS             1376..2554
                     /locus_tag="PLQ18_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018457765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HNE01901.1"
                     /translation="MELPSLNFALGDTIDALRDTVRAFAQAEIAPRAAAIDRDNRFPA
                     DLWPRLGELGLLGITAPEDFGGAGMGYLAHMVAMEEISRASAAVGLSYGAHSNLCVNQ
                     LRRNGSAAQQARYLPKLISGEHVGALAMSEPGAGSDVLAMSLRAERRGDHFVLNGAKM
                     WITNGGDADTLVVYAKTDPDAGARGLTAFILEKDMPGFRHGPHLDKLGMRGSNTYPLF
                     FNDCEVPEDNVLGGVGNGVRVLMSGLDYERAVLAAGPLGIMQACMDVAVPYLHERRQF
                     GQAIGEFQLMQGKIADLYTTLSACRAYVYALGQALDRLGDQHVRSLRKDAAGAILYAA
                     EKATWMAGETIQILGGNGYVNDYPAGRLWRDAKLYEIGAGTSEIRRMLIGRELFEETA
                     "
     gene            2574..>2749
                     /locus_tag="PLQ18_09360"
     CDS             2574..>2749
                     /locus_tag="PLQ18_09360"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237874.1"
                     /note="Catalyzes the synthesis of acetoacetyl coenzyme A
                     from two molecules of acetyl coenzyme A. It can also act
                     as a thiolase, catalyzing the reverse reaction and
                     generating two-carbon units from the four-carbon product
                     of fatty acid oxidation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HNE01902.1"
                     /translation="MKDPVVIVGQARTALGEFRGALSALGAPALGAVAIRAAVTRAGV
                     DAQAIDAVFMGCVL"
BASE COUNT          463 a          987 c          868 g          431 t
ORIGIN      
        1 ggcgacgatg tcgtgcagcg tcgaggtgat cgccagcgga tcgggaacct ggatttcgaa
       61 tttctcggcc acacccatct tcgcccctcc ggcaggaccc ggcacgcttg gcgtttacgt
      121 aaacgcaaac ggccgggcat cgggcaagta aacgccagcg gatggggcga ttcaagacaa
      181 cggaccatga cagcagtcgc gccaccggcg gacggtggtg cgactcgggg cgactcggca
      241 aagattccgt tacaggtaag gacaggccgt gaagtcagtg cagcacttcg tgaccggctt
      301 cgaatttcgc cagcaccgca cgcagatgca gcgcgtgcag tacggcgccg tgcgcctcgg
      361 cgtgacggat gtcatcgagc aggatgcggc gcatcagcgg cacgccctcg gtcgtttcgc
      421 agaggcagca gccgaattcg gccgcgacga tttccggtac atgctcgtgt tcggcaatgg
      481 cttcgatctc gtcttcggtc aggcccgcga acccgacaca atcgacataa gacaacatga
      541 tggctctccc gacaaacgtg ctttccggct tcctcctccc aagcaaagca cgccggccgg
      601 accttgcaat gaaatgtttc tatgcccatc agcaacttga aagcaaacat cggcctgccg
      661 gcctgttgca gccggagccg gtcgattgac gtatacgtca atctgtttca ccttcgcccg
      721 ccatccgatc cccgccccaa cctctggaga gcgagcttga acaccggcga caagaccgcg
      781 ggcacgacct acacgatcag tcagctcgcc cgggaattcg acgtgacgac gcgggccatc
      841 cgcttctacg aggatcaggg tctgctcatt ccggagcgcg tcggccgccg gcgcatctac
      901 gcccgtcgcg accgcacgcg cctgaagctg atcctgcgcg gcaagcgtct cggcatgacg
      961 ctcggcgaaa cccgggaact gtttgatctg tttgacagct cgcgtgacga ggcccgtcag
     1021 ctcgaacgct tcctgcgcat cctcggtgag cgccgcgaca tcctcgtgca gcagcagcgc
     1081 gacatcgagg ccgtgctgca cgagatcaac gccgccgctg ccgagtgcga gcgcctgctc
     1141 gccgaacacg ccgaaccgtc gccgcaggac ccggccggac cgaccgtccc cgcctgaacg
     1201 gccagcgccc tgccccctgc ccctgcgcca ggcggtcgac gttcacgtca acgtcaccta
     1261 gcctgccggt gactgccccg ccggccgggc agccgggcct gcaccctgat gcctgcacgg
     1321 ccccgcccct gatccgatcc cgcgccggcc cggcccgctc cggagcacga cgatgatcga
     1381 actgccctcc ctgaacttcg cgctcggcga caccatcgac gccctgcgcg acacggtgcg
     1441 cgccttcgcg caggccgaga tcgccccgcg tgccgcggcg atcgatcgcg acaaccgctt
     1501 tccggccgat ctctggccac ggctcggcga actcggcctg ctcggcatca ccgcccccga
     1561 ggacttcggc ggtgccggca tgggctatct cgcgcacatg gtggcgatgg aggaaatctc
     1621 gcgtgcttcg gctgccgtcg gcctgtccta cggggcgcat tcgaatctct gcgtgaacca
     1681 gctccgccgc aacggcagcg cggcccagca ggcccgctac ctgccgaagc tgatcagcgg
     1741 cgagcacgtc ggcgcgctgg cgatgagcga gcccggtgcc ggctccgacg tgctggcgat
     1801 gagcctgcgc gccgagcgcc gcggcgatca cttcgtgctg aacggcgcga agatgtggat
     1861 caccaacggc ggcgatgccg acacgctggt cgtctatgcg aagaccgacc cggatgccgg
     1921 cgcgcgcggc ctcacggcct tcatcctcga gaaggacatg ccgggcttcc gccatggccc
     1981 gcacctcgac aagctcggca tgcgcggttc gaacacctat ccgctgttct tcaacgactg
     2041 tgaggttccg gaggacaacg tgctcggcgg cgtcggcaac ggtgtgcggg tgctgatgtc
     2101 cggactcgat tacgaacgcg cggtgctcgc cgccggaccg ctcggcatca tgcaggcctg
     2161 catggacgtg gccgtgccct acctgcacga acgccgccaa ttcggtcagg cgatcggcga
     2221 gttccagctg atgcagggca agatcgccga tctgtacacg acgctgtcgg cctgccgcgc
     2281 ctacgtctac gcgctcggcc aggcgctcga ccgcctcggt gaccagcacg tgcgctcgct
     2341 gcgcaaggat gccgccggcg cgatcctcta cgccgccgag aaagccacct ggatggccgg
     2401 cgaaaccatc cagatcctcg gcggcaacgg ctatgtcaac gactacccgg ccgggcggct
     2461 gtggcgcgac gccaagctct acgagatcgg cgccggcacc tcggagatcc gccgcatgct
     2521 gatcggccgc gaactgttcg aagaaaccgc ctgaaccgcg cccggccctc accgtgaagg
     2581 acccggtcgt catcgtcggc caggcacgca ccgcgctcgg cgaattccgc ggcgcgctgt
     2641 cggcgctggg cgctcccgca ctcggtgccg tggcgatccg cgccgccgtc acgcgggccg
     2701 gcgtcgacgc gcaggccatc gatgccgtgt tcatgggctg cgtgctgag
//
LOCUS       DAOQJH010000400         2747 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_25744_length_2747_cov_3.983655, whole genome shotgun sequence.
ACCESSION   DAOQJH010000400 DAOQJH010000000
VERSION     DAOQJH010000400.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2747)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2747)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2747
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25744_length_2747_cov_3.983655"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..499
                     /locus_tag="PLQ18_09365"
     CDS             <1..499
                     /locus_tag="PLQ18_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007041463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HNE01903.1"
                     /translation="IAAARHAGVRPQDAIAALGRFRGVARRMELRGEAAGVRVYDDFA
                     HHPSAIATTLAGLRASLDAGRRIVAVLEPRSNTMKLGVFKDALAGSLAAADAAVFHAA
                     PDLGWDLAASVASMGSRASIATTIDATVAAAVARVQPGDVLLVMSNGGFGGIHGRLLA
                     ALAGG"
     gene            502..1659
                     /locus_tag="PLQ18_09370"
     CDS             502..1659
                     /locus_tag="PLQ18_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013378233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNE01904.1"
                     /translation="MYVSRLALQNWRNFKQAEGAFGARTFIIGPNASGKSNLLDALRF
                     LRDVAAIGLRQSVLDKRGDVSALRCLAATYRSDVQIEVTLVEDDGPDAGSGWRYRLAF
                     NKDHRQEQPVIRCERVWHGEELLVERPNEEDRSDPERLTQTYLEQINANRGFRELAEF
                     FRSIAYRHPVPEVIRDPKGFSSAPVINDPYGRDFLQRLWQTPQRTREARLRRINQVLQ
                     VAVPQFRELRVEQDETGTPHLVGLYEHWRPNAARQSEAQFSDGTLRLLGLLWALLEDG
                     GPLLLEEPELSLHAAVVAQLPGLFERARRRKGGGRQVIVTTHSEDLLRDPGIAAEEVL
                     WLRPTAQGTLIHQPGDKERLLMQQGMSAADAVLPLSRPDGAEQLALALAPS"
     gene            1660..2280
                     /locus_tag="PLQ18_09375"
     CDS             1660..2280
                     /locus_tag="PLQ18_09375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE01905.1"
                     /translation="MRWPVDVLVEGPTDLFVARRLLHACGLEVGTVYPQRGCGYVCTM
                     APKLAPATVHAPLLVLLDYMDVEEPGDCPPRVVQRHRPALIRPEHYLLRLVVRELESW
                     LLADREAIAGWLGIGLARIPEDPQAEPDPKRTLVNLARLSRRRALREALVPRPGHAAV
                     VGPDYVGQTGRFIQEIWRPDRARRRAGSLDRCLLRLDDLRVRLDGG"
     gene            2434..>2747
                     /locus_tag="PLQ18_09380"
     CDS             2434..>2747
                     /locus_tag="PLQ18_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003491729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HNE01906.1"
                     /translation="MLPYWIALVLTLTAAAASAADPCRDPQTQAEMTRCAGEDYARAD
                     RELNAVYAQVRTALPEAERERLKLAQRAWIASRDADCELEPYGARGGSMEPMLMAACK
                     ADR"
BASE COUNT          395 a          943 c          960 g          449 t
ORIGIN      
        1 gattgccgcg gcccgccacg ccggcgtgcg cccgcaggac gccatcgccg cgctcgggcg
       61 cttccgcggc gtggcgcggc gcatggaact gcgcggcgag gccgctggcg tgcgcgtcta
      121 cgacgacttc gcgcaccacc cgagcgcgat cgcgaccacg ctcgccggcc tgcgcgccag
      181 cctcgatgcc ggccggcgca tcgtcgccgt gctggagccg cgctcgaaca cgatgaagct
      241 cggcgtgttc aaggacgcgc tggccggctc gctcgcggcc gccgatgccg cggtgttcca
      301 cgccgcgccc gatctcggct gggatctggc cgcgagcgtc gcgtcgatgg gcagccgcgc
      361 gagcatcgcc acgacgatcg acgcgaccgt cgccgcggcc gtcgcccgcg tgcagcccgg
      421 cgacgtgctg ctggtgatgt cgaacggcgg cttcggcggc atccacggcc gcctgctggc
      481 ggcgctggcg ggcggctgag catgtatgtc agtcgcctcg ccctgcagaa ctggcggaac
      541 ttcaagcagg ccgaaggcgc gttcggtgcg cgcaccttca tcatcggccc gaacgcatcg
      601 ggcaagtcca acctgctgga tgccctgcgc ttcctgcgcg atgtggcggc catcggcctg
      661 cgtcagtccg tgctcgacaa acggggggac gtgtcggcgc tgcgctgtct ggcggcgacc
      721 taccgttcgg atgtgcagat cgaggtgacc ctcgtggagg acgacggtcc ggatgccggc
      781 tccggctggc gctaccgtct ggcgttcaac aaggatcatc gccaggagca gccggtcatc
      841 cgctgcgagc gtgtctggca cggcgaggag ctcttggtcg agcgcccgaa cgaggaagat
      901 cgcagcgacc cggagcgcct gacccagacc tatctcgaac agatcaacgc caatcgcggt
      961 ttccgtgagc tggccgaatt cttccgcagc atcgcgtacc ggcatccggt gccggaagtg
     1021 atccgcgatc ccaagggctt cagctccgca ccggtcatca acgacccgta cggccgggat
     1081 ttcctgcagc ggctctggca aaccccgcag cgtacccgcg aagcccggct gcggcgcatc
     1141 aaccaggtcc tgcaggtggc cgtgccgcag tttcgggagc tgcgcgtcga acaggacgag
     1201 acgggcaccc cgcatctggt cggcctttac gaacactggc gcccgaacgc ggcgcggcag
     1261 tccgaggcgc agttttcgga tggcacgctg cgtctgctcg gtctgttgtg ggcgctgctc
     1321 gaagacggcg ggccgctgct gctcgaagaa ccggaactgt ccctgcacgc agccgtcgtc
     1381 gcgcagttgc cggggctgtt cgagcgtgcg cgccggcgca agggcggggg ccggcaagtc
     1441 attgtcacga cgcattccga ggatttgctg cgtgacccag gcatcgcggc cgaggaagtg
     1501 ctctggctgc gaccgaccgc acagggcacg ctgatccacc agccgggcga caaggaaagg
     1561 ctgctgatgc agcagggcat gagtgctgcc gatgccgtgc tgcccttgtc gcgcccggac
     1621 ggcgcggagc aactggcgct ggcactggcg ccgtcctgaa tgcgatggcc ggtcgatgtg
     1681 ctggtggaag gaccgacgga cctgttcgtc gcccggcgcc tgcttcatgc ctgcggtctg
     1741 gaggtcggca ccgtgtatcc gcagcgcgga tgcggctacg tatgcacgat ggcaccgaag
     1801 ctggcaccgg ccaccgtcca cgcgcccctg ctggtcctgc tcgattacat ggatgtggaa
     1861 gaacccggcg actgtccgcc cagggtcgtt cagcgtcatc gacccgcact gatccggcct
     1921 gaacactatc tgctgcggct cgtcgtgcgc gagctcgaaa gctggctgct cgcagaccgg
     1981 gaggcgatcg ccggatggct gggcatcggc ttggcccgta ttccggagga tccgcaagcc
     2041 gagcccgatc cgaagcggac gctggtgaat ctcgcccggc tgtcgcgccg ccgggccttg
     2101 cgtgaggccc tcgtgccgcg cccgggccat gcggcggtcg tcggaccgga ttatgtgggc
     2161 cagaccggac gcttcatcca ggagatctgg cgtccggatc gtgcccggcg gcgcgccggc
     2221 agcctcgatc gttgcctgct ccggctggac gatctgcgcg tgcgccttga tggcggataa
     2281 gacatgcagc cgatccctgg gtgtctgctg gatttcgaac tcgcgccaca ccgttcgatc
     2341 gtcgacactg atcactttga ctgcctgcct tggccgctgc cggattgcat tggtttgcgt
     2401 gcccgcccgc cctgtatccg gagttgcgtc gatatgctgc cgtactggat tgccctcgtg
     2461 ctcaccctga ctgctgctgc agcgagtgcc gccgacccct gccgcgatcc gcagacccag
     2521 gccgagatga cccgctgtgc cggcgaggac tacgcgcgcg ccgatcgcga gctgaatgcc
     2581 gtctacgcgc aggtgcgcac cgcgctgccc gaggccgagc gcgagcggct gaagctcgcg
     2641 cagcgcgcgt ggatcgcctc gcgcgacgcc gactgcgaac tggaaccgta cggtgcgcgc
     2701 ggcggttcga tggagccgat gctgatggcc gcgtgcaagg ccgatcg
//
LOCUS       DAOQJH010000401         2747 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_25753_length_2747_cov_2.669019, whole genome shotgun sequence.
ACCESSION   DAOQJH010000401 DAOQJH010000000
VERSION     DAOQJH010000401.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2747)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2747)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2747
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25753_length_2747_cov_2.669019"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..201)
                     /locus_tag="PLQ18_09385"
     CDS             complement(<1..201)
                     /locus_tag="PLQ18_09385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE01907.1"
                     /translation="MNTNTNVNARTAKHLRLIAAAAVLYAGFGAVAHAGDADSVYGEE
                     WRAVLQDRPGSYGYADRHMQPHM"
     gene            complement(396..1244)
                     /locus_tag="PLQ18_09390"
     CDS             complement(396..1244)
                     /locus_tag="PLQ18_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010959585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HNE01908.1"
                     /translation="MSDDMLIDTLRAELAAGRVVPFLGPGLLALCADGSAVPASPLEL
                     VGVMTRKVSVPHKLKNKLPGAAQYIENFKHRKTLKNLMDTAYAPAVTPSPLHAWLAAQ
                     PLPLIVTTWYDDVMGAALASRSDWGRIQGVSQAEHRDGWTHAFAADGTRVEAGAAEAW
                     TTLLYQPTGAVAPAGNYIVSDSDYVEVLTEIDIQTPIPERVQQLRSERSFLFLGCRFD
                     DQLARSWARQISKRSAGRHYAVLPEEPTKNELRFMEEYGIERIELPLAEFAARLTGAG
                     AIAEPA"
     gene            complement(1425..1691)
                     /locus_tag="PLQ18_09395"
     CDS             complement(1425..1691)
                     /locus_tag="PLQ18_09395"
                     /inference="COORDINATES: protein motif:HMM:NF040002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6129 family protein"
                     /protein_id="HNE01909.1"
                     /translation="MIQPAELADIGQSLETLTGTVGDLARALRERYPHLSFSVCDASD
                     VQEDPIAQTGRFDLHLIDAREHCLRLTQQFEQATGVLLAARRLH"
     gene            complement(1694..1906)
                     /gene="nifT"
                     /locus_tag="PLQ18_09400"
     CDS             complement(1694..1906)
                     /gene="nifT"
                     /locus_tag="PLQ18_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004448261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative nitrogen fixation protein NifT"
                     /protein_id="HNE01910.1"
                     /translation="MKVMIRRDSAGKLSVYVPKKDLEEPVVEQEKDDLWGGTVRLANG
                     WLLGLPEMAADTRLPITVEARRLEGD"
     gene            complement(1903..2196)
                     /locus_tag="PLQ18_09405"
     CDS             complement(1903..2196)
                     /locus_tag="PLQ18_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009568025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen fixation protein NifZ"
                     /protein_id="HNE01911.1"
                     /translation="MIESTTPRYDWGLQVTVTDHVYNDDGTFPDVEEDALIIAAGSIG
                     EIVRVGLHEESNMPVYLVDFGIRVVGLSEDEIVPYEGDGSAPVSETSPPGVPA"
     gene            complement(2203..2490)
                     /locus_tag="PLQ18_09410"
     CDS             complement(2203..2490)
                     /locus_tag="PLQ18_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen fixation protein NifZ"
                     /protein_id="HNE01912.1"
                     /translation="MKISRDSDVVELNGPPHFEFGEKVKSRRNIRNDGTFPGREVGDI
                     LVRKGDEGYVVSIGTFLQQFYIYGVEFVDEGYKVGMRRKELESLDYDDDED"
     gene            complement(2505..>2747)
                     /locus_tag="PLQ18_09415"
     CDS             complement(2505..>2747)
                     /locus_tag="PLQ18_09415"
                     /inference="COORDINATES: protein motif:HMM:NF013938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE01913.1"
                     /translation="IWVLTMHADADAAVRCEVARRAPVERLGLMARDADCRVRWEVAL
                     RALPDVLMRLLADAVEPVRELAGRRLDEPDYLAEEA"
BASE COUNT          422 a          915 c          952 g          458 t
ORIGIN      
        1 catgtgcggc tgcatgtgcc ggtcggcgta gccgtagctg ccggggcgat cctgcagcac
       61 cgcgcgccat tcctcgccgt agacgctgtc ggcatcgccg gcgtgggcga cggcgccgaa
      121 gccggcgtac agcacggcgg cagcggcgat caggcgcagg tgcttggcgg tcctggcatt
      181 cacattcgtg ttcgtgttca tggcggcgct cctcggggaa ggatttcggg atcgggacgg
      241 gagcgaacaa gctccacact tccctattcg cgcgcagccg gccggtggat gcagcaaccg
      301 gcaaaaatct ccccccgggc acgaagccgg cacaaaagcc gaccgccgca gtcccggcgc
      361 tgcttccgga actgcggcgg tcggtatggc gaggatcagg ccggctcggc gatggcaccg
      421 gcgccggtca gacgcgccgc gaattcggcg agcggcagct cgatgcgctc gatgccgtat
      481 tcctccatga agcgcagctc gttcttcgtc ggctcttcgg gcagcaccgc gtagtggcgc
      541 ccggccgagc gcttgctgat ctggcgcgcc cagctgcgcg cgagctgatc atcgaagcgg
      601 cagccgagga acaggaagct gcgctcgctg cgcagctgct gcacgcgctc cgggatcggc
      661 gtctggatgt cgatctcggt cagcacctcg acgtaatccg aatccgagac gatgtagttg
      721 ccggccggag caacggcgcc ggtcggctgg tacagcagcg tcgtccaggc ttcggcggcg
      781 cccgcctcga cgcgcgtgcc gtcggcggcg aaggcatgcg tccagccgtc gcggtgctcg
      841 gcctgcgaca cgccctggat gcggccccag tcgctgcgcg acgcgagcgc ggcgcccatc
      901 acgtcgtcgt accaggtcgt gacgatcagc ggcagcggct gcgcggcgag ccacgcatgc
      961 agcggcgacg gcgtcaccgc cggcgcgtag gccgtatcca tcaggttctt cagcgtcttg
     1021 cggtgcttga agttctcgat gtactgcgcg gcacccggca gcttgttctt gagcttgtgc
     1081 ggcaccgaaa ccttcctggt catgacgccg accagttcca gcggcgaggc cggcacggcg
     1141 ctgccatcgg cgcagagcgc cagcaggccg gggccgagga agggtacgac gcggccggcg
     1201 gccagttcgg cgcgcagggt atcgatcagc atgtcgtcgc tcatcgcggc ggactcctgg
     1261 aggcggttgc gttcggttgc gagcaggccc tggggcccgt tcgcgaagct ctgctgccgg
     1321 ccggcgcgcc ggcgccggcg acggtcgcgg tccgtcgccg gccgccggtg ccgtccccgc
     1381 gggtgacgga accggcgcga cggcatcgcg ggatcagtgc ggtttcagtg cagccgacgc
     1441 gcggccagca gcacgccggt ggcctgctcg aactgctgcg tcaggcgcag gcagtgctcg
     1501 cgggcatcga tcaggtgcag gtcgaagcgc ccggtctgcg cgatcgggtc ttcctgcacg
     1561 tcgctcgcgt cgcacaccga gaagctcagg tgcggatagc gttcgcgcag cgcacgggcc
     1621 agatcgccga ccgtgccggt cagggtttcg aggctctggc cgatgtcggc aagttcggcg
     1681 ggctggatca tggtcagtct ccttcgagcc ggcgcgcttc gacggtgatg ggcaggcggg
     1741 tatcggcggc catttccggc aggccgagca gccagccgtt ggcgaggcgc acggtgccgc
     1801 cccacaggtc gtccttttcc tgttcgacga ccggctcttc cagatccttc ttgggcacgt
     1861 acaccgacag cttgccggcc gaatcgcggc ggatcatgac tttcatgcag ggactcccgg
     1921 aggactggtt tcggaaacgg gtgcggagcc atcgccctcg tacgggacga tctcgtcttc
     1981 ggaaagcccg acgacgcgga tgccgaaatc gaccagatac accggcatgt tggattcctc
     2041 gtgcaggccg acgcgcacga tctcgccgat gctgccggcg gcgatgatca gcgcgtcttc
     2101 ctcgacatcc ggaaaggtgc cgtcgtcgtt gtaaacgtgg tcggtgacgg tgacctgcag
     2161 gccccagtca taacgcggcg tggtggattc gatcatggcc gttcagtcct cgtcgtcgtc
     2221 gtaatccagc gattcgagtt ccttgcgccg catcccgacc ttgtagcctt catcgacgaa
     2281 ctcgacgccg tagatgtaga actgctgcag gaaggtgccg atcgatacga cgtaaccctc
     2341 gtcgcccttg cgcacgagga tgtcgccgac ttcccggccc ggaaacgtgc cgtcattacg
     2401 gatgttgcgc cgggacttga ccttctcgcc gaattcgaaa tgcggcggtc cgttcagctc
     2461 gacgacatcg gaatcacgcg aaatcttcat gatggaatca ggcctcaggc ttcttcggcc
     2521 agataatccg gctcgtcgag gcgacggccg gcgagctcgc gcaccggctc gaccgcatcg
     2581 gcgagcaggc gcatcagcac atcgggcagc gcgcgcagcg cgacttccca gcgcacgcgg
     2641 cagtcggcat cacgagccat caggccgagg cgctcgaccg gggcgcggcg cgcgacctcg
     2701 cagcgcacgg cggcatcggc atcggcgtgc atcgtcagca cccagat
//
LOCUS       DAOQJH010000402         2743 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_25799_length_2743_cov_3.735863, whole genome shotgun sequence.
ACCESSION   DAOQJH010000402 DAOQJH010000000
VERSION     DAOQJH010000402.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2743
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25799_length_2743_cov_3.735863"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            305..568
                     /locus_tag="PLQ18_09420"
     CDS             305..568
                     /locus_tag="PLQ18_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004526661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HNE01914.1"
                     /translation="MYRIEFTRPALKDLRALPASLVATLRTKLEAVAAEPYGAHPNVK
                     RLSGSTGYRLRVGDWRVLYELEDDRLVVLVLRVAPRGQAYKRR"
     gene            572..949
                     /locus_tag="PLQ18_09425"
     CDS             572..949
                     /locus_tag="PLQ18_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011325399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNE01915.1"
                     /translation="MNEQVQFIEVDGQPAFAVVPVALWRRLAARAEDIEDADAIAQAK
                     AADDGARIPGAVAFAIAGGTHPVRAWREHHAMTQAALAQAAGISKGYLSQVEAGRRTG
                     TVDTLRALAQALGVTLADLDGSD"
     gene            complement(995..1642)
                     /locus_tag="PLQ18_09430"
     CDS             complement(995..1642)
                     /locus_tag="PLQ18_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008578112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HNE01916.1"
                     /translation="MSRRVRRLLTVVLVLVLTPALLMAWFMARVAIYADNRDEGQADA
                     AVVLGAAAWFTRPSPVFEERIKHALDLYKEGRVRAIVFTGGVGRGDTRSEAEVARNYA
                     LRRGVPESAIHVETASRSTFGNLVNARLLLERNGLRRVLLVSDPLHMRRAMTMARDLG
                     IDARPSPTPTTRFQSFSTQFDLLLKETFDYARYKLGRHFLDFDEPTAGYAAAGES"
     gene            complement(1671..2327)
                     /locus_tag="PLQ18_09435"
     CDS             complement(1671..2327)
                     /locus_tag="PLQ18_09435"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009555641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNE01917.1"
                     /translation="MKAIQLTETLYDYLIDVSLRETDVQRRLRAYTSTLERARMQIAP
                     EQGQFMQWLVRLTGARRIVEVGTFTGYSALAMALALPPDGRLIACDIDPVHTATAQQY
                     WQEAGVSDRIELRLGPALDTLDGLITEFGRNKIDLAFIDADKTAHLFYYERLLEMLRP
                     GGVILIDNTLWGGSVADPAAHDADTLAIRGLNLLLRDDDRIDLAHLPVGDGLALALKR
                     "
     gene            2405..>2743
                     /locus_tag="PLQ18_09440"
     CDS             2405..>2743
                     /locus_tag="PLQ18_09440"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNE01918.1"
                     /translation="MKPESAVRIVQISDCHLRSTPGAQSWGVDVDAGLRAVLTLATGL
                     QPALLLATGDLVHDEGTPAYERLRDDFLLPSGLPVAALPGNHDDSDALAACWSSGPVQ
                     RRRQFEAGGWQ"
BASE COUNT          409 a          926 c          999 g          409 t
ORIGIN      
        1 atgcgcggcc ggcatgtgcg tctgccgccg gtgacgccgg acctgttcgg cgatgacggt
       61 gcgtcatgag cgcgcaagcg caagtccacc cgcaggaggg cgcccgcacg ggcgcccgtc
      121 ccggctttcc ggtcgtgccc gctgctgcgc tcgctgcgcg tgcgcctgat gcctgcttcg
      181 agcgcgagct acaggagttt cgggactact ggcgcacgca cgtcgaggaa cgggcagggt
      241 agcggcgcgg ggcgggcaaa gcgttttgct gacgtgaccg ttagcggatt gctaatatgc
      301 gtgcatgtac cgcatcgagt tcacgcgccc ggcgctgaaa gacctgcgcg cgctgccggc
      361 atctctggtg gcgacgttgc gcacgaaact cgaagcggtc gcggccgagc cttacggcgc
      421 acacccgaac gtcaaacgac tgagcggctc gaccggctac cggttgcggg tcggtgactg
      481 gcgggtgctg tacgaactgg aggatgaccg gctggtggtg ctggtgctgc gcgtggcccc
      541 gcgcggacag gcttacaagc gaaggtgagg catgaacgaa caggtgcagt tcatcgaagt
      601 ggacgggcag ccggcgttcg ccgtcgtccc ggtcgccctg tggcgcaggc tagccgcacg
      661 ggccgaggac atcgaggacg ccgacgccat cgcgcaggcg aaagccgccg atgacggcgc
      721 gcgcattccg ggtgccgtcg ccttcgccat cgcgggcggt acgcatccgg tgcgggcgtg
      781 gcgcgagcac cacgccatga cgcaagccgc cctcgcacag gcggcgggca tctcgaaggg
      841 ctacctgtcg caagtcgagg ccggccggcg caccgggacc gtggacacgc tgcgcgcact
      901 ggcgcaggcg ctcggcgtga cgcttgcgga cctcgacggc agcgactgaa ccctggcgcc
      961 cgatgccctt gccggcgcgc atcggccgtg ccggtcacga ctcgcccgcg gccgcgtagc
     1021 cggcggtcgg ctcgtcgaag tccaggaagt ggcggccgag cttgtagcgg gcgtagtcga
     1081 aggtctcctt cagcagcagg tcgaactgcg tcgagaagct ctggaagcgc gtggtcggtg
     1141 tcggtgacgg gcgggcatcg atgccgaggt cgcgggccat cgtcatcgcg cggcgcatgt
     1201 gcagcgggtc cgagaccagc agcacgcgcc tgaggccatt gcgctccagc agcagccggg
     1261 cgttgacgag gttgccgaag gtcgagcgcg aggcggtctc gacgtggatg gccgactccg
     1321 gcacgccgcg gcgcagcgcg tagttgcgcg cgacctcggc ctcggagcgc gtgtcgccgc
     1381 gcccgacgcc gccggtgaac acgatcgcgc gcacgcggcc ttccttgtag agatcgagcg
     1441 cgtgcttgat gcgctcctcg aacaccggcg acggccgcgt gaaccaggcg gcggccccga
     1501 gcacgaccgc cgcatcggcc tggccttcat cgcggttgtc ggcgtagatc gccacgcgcg
     1561 ccatgaacca ggccatcagc agcgccggcg tcagcaccag caccagcacg acggtcagca
     1621 ggcggcggac gcggcggctc atcttcggta tcggtcctca cggccagtgg tcagcgcttc
     1681 agcgccagcg ccagaccgtc gccgaccggc aggtgcgcca ggtcgatgcg gtcgtcatcg
     1741 cgcagcagca ggttcaggcc acggatcgcc agcgtgtcgg cgtcgtgcgc ggccgggtcg
     1801 gcgacgctgc cgccccagag cgtgttgtcg atcaggatca cgccgccggg gcgcagcatt
     1861 tccagcaggc gctcgtaata gaagaggtgc gcggtcttgt cggcatcgat gaaggccagg
     1921 tcgatcttgt tgcggccgaa ctcggtgatc agcccgtcga gtgtgtcgag tgccggaccg
     1981 aggcgcagct cgatgcgatc ggagacgccg gcctcctgcc agtactgctg cgcggtcgcg
     2041 gtatgcaccg ggtcgatgtc gcaggcgatc agccggccgt ccggcggcag cgccagcgcc
     2101 atcgccagcg cgctgtagcc ggtgaaggtg ccgacctcga cgatgcgccg cgcgccggtc
     2161 aggcgtacca gccactgcat gaactggccc tgttcgggcg cgatctgcat gcgcgcgcgc
     2221 tccagcgtcg acgtgtacgc gcgcagtcgc cgctgcacgt cggtctcgcg cagcgacacg
     2281 tcgatcaggt agtcgtacag ggtctcggtc agctggatgg ctttcatggg gacgcggcgc
     2341 tccggcggcg gggggacaat gatagtcata tacccgtatc ccgccgattg ccggagcctt
     2401 gccgatgaaa cccgaatccg ccgtgcgcat cgtgcagatc agcgactgtc acctgcgcag
     2461 cacgccgggc gcgcagagct ggggtgtcga tgtcgatgcc ggcctgcgcg ccgtgctgac
     2521 cctcgcgacc gggctgcaac cggcgctgct gctcgcgacc ggcgatctgg tccacgacga
     2581 aggcacaccg gcctacgaac gcctgcgcga tgacttcctg ctgccgagcg gcctgccggt
     2641 cgcggctctg cccggcaacc acgacgactc cgacgccctc gccgcctgct ggagttcagg
     2701 tccggtgcag cggcgccggc agttcgaagc cggcggctgg caa
//
LOCUS       DAOQJH010000403         2741 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_25835_length_2741_cov_3.401713, whole genome shotgun sequence.
ACCESSION   DAOQJH010000403 DAOQJH010000000
VERSION     DAOQJH010000403.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2741)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2741)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2741
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25835_length_2741_cov_3.401713"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            374..904
                     /locus_tag="PLQ18_09445"
     CDS             374..904
                     /locus_tag="PLQ18_09445"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNE01919.1"
                     /translation="MSEEWFDVVDEHDRPLGRALRAEVHRLGLRHRSVHMLVFNAAGS
                     VFLQKRSLAKDNCPGLWDVSAAGHVGAGEDYDAAAPRELAEELGCVPAVAPELLFALE
                     AAEHTGWEFVRVYRVEAEGPFELDPVEIAEGRWFTPAEVDAWLAGRAAETTATLRIIW
                     SRVRAAAAGLPGADGR"
     gene            complement(901..2115)
                     /locus_tag="PLQ18_09450"
     CDS             complement(901..2115)
                     /locus_tag="PLQ18_09450"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HNE01920.1"
                     /translation="MSPLFPPPPEAGPGDDAMRLEIEAIADGVCRVLDGDFSIRVGTG
                     SADHTVQKLAMLINFLLATGERQTERLNRMNALLEARVAARTRELEEAQMQLRRALGR
                     LEATHAHSEALFDAALDVIITIDTGGIIRSANRATRATLGFEPGELLGRNVSVLMPAP
                     MRDEHDAYLHRYLATGEARVINVGREVTVRRRDGSTYLAFLTISEYRIGGRPYFVGFL
                     RDIAYFKQREQQLRRLAELDPLTGVPNRSGIIYALERLLTETGEQAPGLVGLFYIDLD
                     DFKPVNDTLGHDYGDALLIAVAERLRRRVRGSDLVGRLGGDEFVVVAGPVPTVGDIEH
                     IRVTLAEALAQPFEVLDRELGLGGSIGVALYPRDGTTPEKLLRVADQSMYATKRRRKQ
                     RRHGPAPDGQAG"
     gene            complement(2087..>2741)
                     /locus_tag="PLQ18_09455"
     CDS             complement(2087..>2741)
                     /locus_tag="PLQ18_09455"
                     /inference="COORDINATES: protein motif:HMM:NF019320.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="heme NO-binding domain-containing protein"
                     /protein_id="HNE01921.1"
                     /translation="FEGAMLGIFHVTLTELLALHGGPGAVARLLEQAGLPAGTRFRID
                     TCYPDTDAERLLATAAQVLGLDAAALHRAYAAHAGAWALRVFPSFFELAPDSREFLRS
                     QPRFAGLLDAGAQCAGPDPLPTGAPGPIEIETPAPDALRIRWRASPGLLALYRELLRW
                     VVRHYRDVADVRMQVNGSGTGMVAIYDLSWIRFAGDTRRHERFETTAPDESPVPPAA"
BASE COUNT          373 a          935 c         1026 g          407 t
ORIGIN      
        1 gagggccggg aaggggggcg gggcagcatg tccgaatcgt ccgtgcggga agggaaaccg
       61 ggctcgccgg tgcgccgctg ccgcagcgac cggtcgatgc gcccgcatgc tacccgcgcc
      121 cgccggacgt gcgcatacgc cgcctgccgg ggtcggaccg gaaaatccgc agccccgtgc
      181 ccgggtttcg caggtgctgg ttcaaattca tgttttatct ggatttcatt cgtgcgggcc
      241 gcccggatgg catccggaga gcccgcaatt aaattacgtc catacacggt ctgtgccgtt
      301 cctgctaggc gcctgccggc ccgccgtggc aaggtgcggc gttgccgccg tccggggcgc
      361 agggtgaaac gtgatgtccg aggaatggtt cgacgtggtc gacgagcacg accggccgct
      421 cgggcgcgcg ctgcgcgccg aggttcaccg gctcgggctg cgtcaccggt ccgtgcacat
      481 gctggtgttc aacgccgccg gctcggtgtt cctgcagaag cgctcgctcg ccaaggacaa
      541 ctgtccgggg ctctgggatg tctcggcggc cggccacgtc ggggccggcg aggactatga
      601 cgcggccgcc ccgcgcgagc tggccgagga gctcggctgc gtgccggccg tggcgcccga
      661 gctgctgttt gcgctcgagg ctgccgagca caccggctgg gagttcgtgc gcgtctaccg
      721 ggtcgaggcc gaagggccgt tcgagctgga cccggtcgag atcgccgagg gccgctggtt
      781 cacgccggcc gaggtcgatg cctggctggc ggggcgcgcc gccgaaacca cggcgacgct
      841 gcgcatcatc tggtcgcgcg tgcgtgcggc cgccgccggc ctgcccgggg ccgacggccg
      901 ctagccggcc tgaccgtccg gcgccgggcc gtgacggcgc tgcttgcgcc ggcgcttggt
      961 cgcatacatc gactggtcgg cgacgcgcag gagcttctcc ggcgtcgtgc cgtcgcgcgg
     1021 gtacagcgca acaccgatgc tgccgccgag gccgagttcg cgatcgagca cctcgaaggg
     1081 ttgcgccagc gcttcggcga gcgtgacgcg gatgtgctcg atgtcgccga ccgtcggcac
     1141 cgggccggcg accacgacga attcatcccc gccgagccgc ccgaccagat cggagccgcg
     1201 cacccggcgc cgcagccgct cggcgaccgc gatcagcagc gcatcgccgt agtcgtggcc
     1261 gagcgtgtcg ttgaccggct tgaagtcgtc gagatcgatg tagaacagcc cgaccagccc
     1321 cggcgcttgt tcgccggttt cggtgagcag gcgctccagc gcgtagatga tgccgctgcg
     1381 gttcggcacg ccggtcagcg ggtcgagttc ggcgagccgg cgcagctgct gctcgcgctg
     1441 cttgaagtag gcgatgtcgc gcaggaatcc gacgaagtac ggccgcccgc cgatgcggta
     1501 ctcgctgatg gtcaggaagg ccagataggt gctgccgtcg cgccggcgca ccgtcacctc
     1561 gcgcccgacg ttgatcacgc gcgcctcgcc ggtggcgaga taacggtgca ggtaagcgtc
     1621 gtgctcgtcg cgcatcggcg ccggcatcag cacgctgacg ttgcgcccga gcagctcgcc
     1681 cggctcgaag ccgagcgtgg cgcgggtggc gcggttggcc gagcggatga tgccgccggt
     1741 gtcgatggtg atgatcacgt cgagcgcggc gtcgaacagc gcctcgctgt gcgcgtgcgt
     1801 ggcttcgagc cgtccgagcg cgcggcgcag ctgcatctgg gcttcctcca gctcgcgcgt
     1861 gcgcgccgcg acgcgcgctt cgagcagcgc gttcatgcgg ttgagccgct cggtctggcg
     1921 ctcgccggtg gccagcagga agttgatcag catcgccagc ttctggaccg tgtgatcggc
     1981 cgagccggtg ccgacccgga tcgagaagtc gccgtcgagc acgcggcaga ccccgtcggc
     2041 gatcgcctcg atttcgaggc gcatcgcgtc gtccccgggg ccggcttcag gcggcggggg
     2101 gaacagggga ctcatcgggt gcggtggtct cgaagcgctc atgcctgcgc gtgtccccgg
     2161 caaagcggat ccaggacaga tcgtagatgg cgaccatgcc ggtgccgctg ccgttcacct
     2221 gcatgcgcac gtcggccacg tcgcggtaat ggcgcacgac ccagcgcagc agttcgcgat
     2281 acagcgccag caggccgggg ctggcgcgcc agcggatgcg cagcgcatcc ggggccgggg
     2341 tttcgatctc gatcggcccg ggggcgccgg tcggcagcgg gtccggcccg gcgcactgcg
     2401 cgccggcatc cagcagtccg gcgaagcgcg gctggctgcg caggaactcg cgcgaatccg
     2461 gcgccagttc gaaaaagctc gggaacaccc gcagcgccca ggcaccggcg tgcgcggcat
     2521 aggcccggtg cagcgccgcg gcgtcgagcc ccagcacctg cgcggcggtg gcgagcagcc
     2581 gttcggcgtc ggtgtccgga tagcaggtat cgatgcgaaa gcgcgtgccg gccggcaggc
     2641 cggcctgttc gagcagccgt gcgacggcgc ccgggccacc gtgcagggcg agcagttccg
     2701 tcagcgtgac gtggaagatc ccgagcatcg ctccctcgaa c
//
LOCUS       DAOQJH010000404         2737 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_25890_length_2737_cov_5.080537, whole genome shotgun sequence.
ACCESSION   DAOQJH010000404 DAOQJH010000000
VERSION     DAOQJH010000404.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2737
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25890_length_2737_cov_5.080537"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            79..531
                     /locus_tag="PLQ18_09460"
     CDS             79..531
                     /locus_tag="PLQ18_09460"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HNE01922.1"
                     /translation="MTDAHRAVVRWQRATADFDAKTFERTHTIVYDGGEQVRASTAPE
                     YGGDASAVDPEQGLVGALASCHMLTFLTIAALKRIVVDAYEDEASAETGRNADGRMMV
                     TRVTLRPRVRFAPGHEQDASALDALHARAHGNCFIANSLRSVVSIEPR"
     gene            complement(550..861)
                     /locus_tag="PLQ18_09465"
     CDS             complement(550..861)
                     /locus_tag="PLQ18_09465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE01923.1"
                     /translation="MLPLWFDMNARVWLHAQHVLLDNVVAMMRLVSGARCPAPVRLAD
                     RCDGCGGALQWRGPQAAPYQRCPACGLIRFHAPLPQLPAGSAISGETHRVLSFRPRRG
                     A"
     gene            1203..1553
                     /locus_tag="PLQ18_09470"
     CDS             1203..1553
                     /locus_tag="PLQ18_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017938676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 pili tip component"
                     /protein_id="HNE01924.1"
                     /translation="MHIRNLIREWERAGVRTLTVESCCVQLPLHDAARLAALAEMYPL
                     KSRAELITELLSAALDQVEAAFPYVQGSRVIAEDEQGDPMYEDAGLTPLFLRLTKRHA
                     ARLAEESAQQKDGA"
     gene            1713..1928
                     /locus_tag="PLQ18_09475"
     CDS             1713..1928
                     /locus_tag="PLQ18_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008316302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine-zipper protein"
                     /protein_id="HNE01925.1"
                     /translation="MKNLIKLSALAGLSVLAVGCASTSDLEAVRAEAQSAKATANQAL
                     ATANEAKSIAMDTNEKLSRMFKKSMYK"
     gene            complement(2018..>2737)
                     /locus_tag="PLQ18_09480"
     CDS             complement(2018..>2737)
                     /locus_tag="PLQ18_09480"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNE01926.1"
                     /translation="LRVYLQSQVDHDGRITGAEALLRWQHPQRGLVPPGEFIPIAECT
                     ELIVQLDRWVLGEVVALLARSAGCRPAPTLSVNVSPRMFASPGFVDELRTLLAPHPDS
                     ARRLVIELTEGVLVHDPEVVAERMAALGALGVRFSIDDFGTGYSSLTYLKRLPLHELK
                     IDRSFILEAPHDADDAALVGLIVSVAGHLRLEVVAEGVETAVHADWLRSHPGVRRQGY
                     LYGRPVPAGDWFGDYASGCDA"
BASE COUNT          466 a          941 c          913 g          417 t
ORIGIN      
        1 ccgcctggcc cgcgccggcg ccagcgtgcc gccggtctgc tgaagtattc cccaagtaat
       61 cgatacggaa gcctcccgat gaccgacgcc caccgggccg tcgtgcgctg gcagcgtgcg
      121 acggccgatt tcgatgcgaa gaccttcgag cgcacccaca ccatcgtcta cgacggcggc
      181 gaacaggtgc gcgcctcgac ggcgccggaa tacggcggcg atgccagcgc ggtcgatccc
      241 gaacaggggc tggtcggtgc gctggcgagc tgccacatgc tgaccttcct gaccatcgcc
      301 gccctgaagc gcatcgtcgt cgatgcctac gaggacgagg ccagcgccga gaccggccgc
      361 aatgccgacg gccgcatgat ggtcacgcgc gtgacgctgc gtccgcgcgt gcgcttcgcg
      421 cccggccacg agcaggacgc ctccgcgctc gatgcgctgc acgcgcgcgc acacggcaac
      481 tgcttcatcg ccaattcgct gcgcagcgtg gtctcgatcg aaccacgctg attttcggcc
      541 ggctgcggct caggcgccgc gtcggggccg gaaggacagg acgcgatgcg tctcgccgct
      601 gatagcgcta ccggccggaa gctgcggcag cggcgcatgg aagcggatca ggccgcaggc
      661 cggacagcgc tggtacggcg ccgcctgcgg gccacgccat tgcagggcac caccgcagcc
      721 gtcgcaacga tcggcgaggc gcaccggcgc cggacatcgc gcaccggaca ccagccgcat
      781 catcgccacc acgttgtcga gcagcacatg ctgggcatgc agccagacgc gggcattcat
      841 gtcgaaccac agcggtaaca ttgagcgttt tctcctgagc ggcgggcctg gtctgtagcc
      901 gggtttgagc gccttgcgga ccggacctgc agccttacgc cacaggtcct gaccacaatc
      961 cccattaagc aattgacgcg ccaaatatta ccgtttgttc attacggtat tttaaggtcg
     1021 cgcgcccggt cgaaaactgg cacaggctct ggatttgtca ataaggcgac tgccgatcgg
     1081 tattccggcc cgggtgacag tggtgcgtca aaatcgtgac atccctgctg ctgccgtcac
     1141 cggatgcagc ggcgccgtac cacgaccata ctgacgccga cccttcccgc ccgaggtatg
     1201 ccatgcacat ccggaatctg atccgcgaat gggagcgcgc cggtgtccgc acgctgacgg
     1261 tcgaatcctg ctgcgtgcag ctgccgctgc acgatgccgc gcggctggcg gcgctcgccg
     1321 agatgtatcc gctgaagagc cgggccgagc tcatcaccga actgctgtcg gccgcgctcg
     1381 atcaggtcga ggcggcgttc ccgtacgtgc agggctcgcg ggtgatcgcc gaggacgaac
     1441 agggcgatcc gatgtacgag gatgccggcc tgacgccgct gttcctgcgc ctgaccaagc
     1501 gtcacgccgc gcggctggcc gaggaatcgg cgcagcagaa ggacggcgcg tgaacggccg
     1561 tgcggccggc gacgcttgcg cggtgcaaca ttatttgtcg ccggcgcgga tcgccggctt
     1621 ggcaaggcaa accggagcga tacactagcg gggctttgtt gcgggcatgt ctgtccgcgt
     1681 tgtttttttg cctgactgtt taggagccga acatgaagaa cctgatcaag ctgtccgccc
     1741 tcgccggcct gtccgtcctc gctgtcggct gcgccagcac cagcgacctc gaagccgtgc
     1801 gcgccgaggc ccagtcggcc aaggccaccg ccaaccaggc gctggcgacc gccaacgagg
     1861 ccaagtcgat cgcgatggac accaacgaga agctgagccg catgttcaag aagagcatgt
     1921 acaagtaagt cgcccgccga ccgaacgtcc tgcttcaaaa aaaggccggg ttccccccgg
     1981 cctttttgct gcccggcacc ctggctctca gggtctctca ggcgtcgcag ccgctcgcgt
     2041 agtcgccgaa ccagtcgccg gccggcaccg ggcggccgta cagatagccc tgccggcgca
     2101 cgccgggatg actgcgcagc cagtcggcgt gcacggcggt ctcgacgccc tcggcgacca
     2161 cctccagacg caggtggccg gccaccgaca cgatcagccc gaccagcgcc gcatcgtcgg
     2221 cgtcgtgcgg tgcttcgagg atgaagctgc ggtcgatctt cagctcgtgc agcggcaggc
     2281 gcttcaggta ggtgaggctc gaatagccgg tgccgaaatc atcgatcgag aagcgcacgc
     2341 caagcgcccc gagcgcggcc atgcgctcgg cgacgacttc gggatcatgc accagcacgc
     2401 cttcggtcag ctcgatgacc agccgccggg cgctgtccgg gtgcggggcg agcagcgtgc
     2461 gcagttcgtc gacgaagccg gggcttgcga acatgcgcgg gctgacgttg accgacagcg
     2521 tcggtgccgg acggcagccg gcgctgcggg caagcagcgc gacgacctcg ccgagcaccc
     2581 agcggtcgag ctgcacgatc agctccgtgc actcggcgat cgggatgaac tcgcccggcg
     2641 gcaccaggcc gcgctgcggg tgctgccagc gcagcagcgc ctcggcgccg gtgatgcggc
     2701 cgtcgtgatc gacctgtgac tgcaggtaca cgcgcag
//
LOCUS       DAOQJH010000405         2733 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_25971_length_2733_cov_4.046303, whole genome shotgun sequence.
ACCESSION   DAOQJH010000405 DAOQJH010000000
VERSION     DAOQJH010000405.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2733)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2733)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2733
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25971_length_2733_cov_4.046303"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..889
                     /locus_tag="PLQ18_09485"
     CDS             <1..889
                     /locus_tag="PLQ18_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010810202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HNE01927.1"
                     /translation="NPVDTKVRAPKAAVEATPRVLGWDAAGTVEAIGSEVTLFRPGDE
                     VWYAGSIVRPGSNAEFQCVDERIVALKPRSLDFAQAAALPLTTITAWEALFERLGLDA
                     DGADAGRSLLIIGGAGGVGSIAIQLARRAGLTTIVTASRPETIEWCRALGATHVIDHR
                     QPLPAQLEALGFRQVDAIANFADTDAYWAVMCELIAPQGRICAIVENRAPLELGLLKA
                     KSAGFVWEFMFTRAQFGTPDMIAQHRLLTRVAGLVDAGELRTTLRETLGRIDAANLRE
                     AHRRLERGTGIGKLVLAGW"
     gene            1007..2038
                     /locus_tag="PLQ18_09490"
     CDS             1007..2038
                     /locus_tag="PLQ18_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) ABC transporter substrate-binding
                     protein"
                     /protein_id="HNE01928.1"
                     /translation="MSRRFPLLCAALFAALGLHALPSAAADAPVLNVYSARHYQTDEA
                     LYAGFTEQTGIRVNRIEGKEDELLERLKNEGAASPADVLVTVDAARLAKADALGLFAP
                     VESKTLAARIPEYLRTPTWIAFSTRARVIVYAKDSVNPADVASYESLADPKLKGKVCS
                     RPGAHPYNLSLMAGLIGHLGEAKATEWAQGLVANFARPPRGGDTDQIKAVAAGECGVA
                     ISNSYYLARLMRSDKPEDQAVVAKVGMVWPNQASYGTHINVSGAGVLKHAPNREAAVK
                     FLEYLASDRAQVYFADGNNEWPAVAGVKVDNPALAKLGPFKSDTQSVGVLAANQAEAL
                     KIFDRVGFR"
     gene            complement(1993..2343)
                     /locus_tag="PLQ18_09495"
     CDS             complement(1993..2343)
                     /locus_tag="PLQ18_09495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE01929.1"
                     /translation="MLLLLLLCCVLIGNRLIIKTFSSLLIRCLLMLPLLIPPAALLWW
                     SFGSSPLLNEPAMLRLRDHAVLAFPLTQLLYLSLLALAPLIEHRLRRQAMSVSAVPEV
                     QRNPTRSKIFSASA"
BASE COUNT          446 a          919 c          936 g          432 t
ORIGIN      
        1 gaatccggtc gacaccaagg tgcgggcgcc gaaggccgcg gtcgaagcga cgccgcgcgt
       61 gctcggctgg gatgccgccg gcaccgtcga ggcgatcggc agcgaagtga cgctgttccg
      121 gcccggggac gaggtctggt atgccggcag catcgtgcgc cccggcagca acgccgaatt
      181 ccagtgcgtc gatgaacgca tcgtcgcgct gaaaccgcgc tcgctcgatt ttgcgcaggc
      241 cgcggcgctg ccgctgacga cgatcaccgc ctgggaggcc ttgttcgagc gtctcggcct
      301 cgatgcggac ggggccgatg ccgggcgcag cctgctgatc atcggcgggg ccggcggcgt
      361 cggttcgatc gcgatccagc tcgcgcggcg cgccggcctg acgacgatcg tgaccgcctc
      421 gcggccggaa accatcgaat ggtgtcgtgc gctcggtgcg acccacgtca tcgatcaccg
      481 ccagccgctg ccggcgcagc tcgaagcgct cggtttccgg caggtcgatg cgatcgcgaa
      541 ctttgccgat accgatgcct actgggcggt gatgtgcgag ctgatcgcgc cgcagggccg
      601 catctgcgcg atcgtcgaga accgcgcgcc gctcgaactc gggctgctga aggccaagag
      661 cgccggtttc gtctgggaat tcatgttcac gcgcgcgcag ttcggcacgc cggacatgat
      721 cgcgcagcac cggctgctga cgcgcgtcgc cgggctcgtc gatgccgggg aactgcgcac
      781 gacgctgcgc gagacgctcg ggcgcatcga tgccgccaac ctgcgcgagg cgcaccgccg
      841 gctggagcgc ggcaccggca tcggcaagct ggtgctggcc ggctggtgag tcatgcggct
      901 gtcatcgcca tgcggatgac agccgttctc atttacagcg ggagatccat tcggcagact
      961 ccgcccgacc ctcgaatggt cgatcacctg taaggagtcc tgtccgatgt cgcgtcgatt
     1021 tccgctgctg tgcgccgccc tgttcgcggc gctcggcctg catgccctgc cctcggccgc
     1081 agccgatgcg ccggtgctga acgtctattc cgcccggcat taccagaccg acgaggcgct
     1141 gtatgccggt ttcaccgagc agaccggcat ccgcgtcaac cgcatcgaag gcaaggagga
     1201 cgaactgctg gagcgcctga agaacgaagg cgccgccagt ccggccgatg tgctggtcac
     1261 cgtcgatgcc gcgcgtctgg ccaaggccga tgcgctcgga ctgttcgcgc cggtcgaatc
     1321 gaagacgctg gccgcgcgca tccccgagta cctgcgcacg ccgacctgga tcgcgttctc
     1381 gacgcgcgcc cgcgtcatcg tctacgccaa ggattcggtg aatccggccg atgtcgccag
     1441 ctatgaatca ctcgccgatc cgaagctcaa gggcaaggtc tgctcgcgcc ccggcgcgca
     1501 cccgtacaac ctgtcgctga tggccggact gatcgggcat ctcggcgagg caaaagcgac
     1561 cgagtgggcg cagggcctgg tcgcgaactt cgcgcgtccg ccgcgcggcg gcgacaccga
     1621 ccagatcaag gcggtcgcgg ccggcgagtg cggcgtggcg atctcgaaca gctattacct
     1681 cgcgcggctg atgcgctcgg acaagcccga ggatcaggcc gtggtcgcga aggtcggcat
     1741 ggtctggccg aaccaggcga gctacggcac gcacatcaac gtctcgggtg ccggcgtgct
     1801 gaagcacgcg ccgaatcgcg aggcggccgt gaagttcctc gaatacctcg cgagcgaccg
     1861 ggcgcaggtc tacttcgccg acggcaacaa cgagtggccg gcggttgccg gcgtcaaggt
     1921 cgacaacccg gcgctcgcga agctcggacc gttcaagtcc gacacgcagt cggtcggcgt
     1981 gctggccgcg aatcaggccg aggcgctgaa gatcttcgac cgcgtcggat tccgctgaac
     2041 ctcgggcact gcggatacgg acatcgcctg ccggcgcagg cggtgttcga tcagcggggc
     2101 gagcgcgagc aggctcagat acagcagctg cgtcagcggg aatgccagca cggcatggtc
     2161 tcgcagcctc agcatcgccg gctcgttcag cagcgggctg ctgccgaagc tccaccacag
     2221 cagcgccgcc ggcggtatca gcagcggcag catcagcagg cagcggatca gcagggagct
     2281 gaaggtcttt atgatcaggc gattgccgat caggacacag cagaggagca aaagcagcag
     2341 catgtcggcg ttccttcgca acggggaggg ccggcgggcg ctgccggcgg ggtgtatcta
     2401 atcctgttgc accatttcag ttcaagctcc ggcatggctc ttctgaactt tttttcacga
     2461 ttccgggcct caagtccgcc cggatcgggc cggggccgcg tccggagccg gcgccggagg
     2521 ctcgacccgc gcaagccgat cccgcgcggg actgcctgaa aacgctgctt gcacccggca
     2581 ggcgccgtct acgcttgttc aaaaccattc aacagaagcc gtcgcacgct gcgcttcgca
     2641 gcgcctgcgt ccggggtttc gctcgcttca catgcctgcc gtccgccgac gacggccacg
     2701 taggtggcgt cgtggcgagc attcgcgcga cgt
//
LOCUS       DAOQJH010000406         2732 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_25989_length_2732_cov_3.269331, whole genome shotgun sequence.
ACCESSION   DAOQJH010000406 DAOQJH010000000
VERSION     DAOQJH010000406.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2732)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2732)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2732
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25989_length_2732_cov_3.269331"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1534)
                     /locus_tag="PLQ18_09500"
     CDS             complement(<1..1534)
                     /locus_tag="PLQ18_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006746174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNE01930.1"
                     /translation="MKLVRKQAGARPAAAATSPVAAFLGATVDRRAFLKSSGLALGAT
                     ALGAALGPTMMRRASAATDAGTHASNAAVEIKRTICSHCSVGCGIYAEVQNGVWVGQE
                     PAFDHPFNMGAHCAKGASIREHGHGERRLKYPMKLVDGQWQRISWDTALNEVSARLLK
                     IREESGPDAVYWLGSAKHSNEQAYLFRKFASMWGTNNVDHQARICHSTTVAGVANTWG
                     YGAMTNSYNDIAYAKSILMIGSNAAEAHPVSMQHILRAKEDGAKLIVIDPRFTRTAAH
                     ADHYVRIRPGTDVPFIWGLLWHVFQNNWQDDSFLQQRVFGMDEVRKEVEKWPPAEVER
                     VTGVQEKDAYTVAKLMAEHRPGTFVWCMGGTQHTIGNNNTRAYCIFQLALGNMGKAGG
                     GTNIFRGHDNVQGATDFGVLADSLPGYYGLAEGSWKHWAKVWGVDYEWLKARFDPNAY
                     DGGSGKPVKPMETSGIPVSRWIDGVLEDKALMTQRDNVRGMVFWGHAPNSQTRGTEMK
                     KAF"
     gene            complement(1545..1760)
                     /locus_tag="PLQ18_09505"
     CDS             complement(1545..1760)
                     /locus_tag="PLQ18_09505"
                     /inference="COORDINATES: protein motif:HMM:TIGR02811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HNE01931.1"
                     /translation="MSEKTPAAGLDRRAFLKLVGSGAGAGAVALALPGVAVAAPAAVR
                     PEPEEAGRLDAGYRETAHVRAYYDTCR"
     gene            complement(1842..2474)
                     /locus_tag="PLQ18_09510"
     CDS             complement(1842..2474)
                     /locus_tag="PLQ18_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014247045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD family protein"
                     /protein_id="HNE01932.1"
                     /translation="MTTMDAAPTTAPDEDDRLRAGTWALLAALLRRPPTAELLAQLAA
                     IDTPPAGEGGALAAAWQDLRAAAAVADPVALEREYHALFIGIGRGELLPFASWYLTGF
                     LMEKPLGELRADLARLGFERQPGICEPEDHIAALAEVLGLLIGEGSAAAAGFFAHHIE
                     PWAGRLFADLQTAESARFYRAAGRFAEAFLAFERQYGAMPALAPSSVPAG"
BASE COUNT          364 a          936 c          972 g          460 t
ORIGIN      
        1 cgaacgcctt cttcatctcg gtgccgcgcg tctgcgagtt cggcgcgtgg ccccagaaca
       61 ccatgccgcg cacgttgtcg cgctgcgtca tcagcgcctt gtcctcgagc acgccgtcga
      121 tccagcgcga caccgggatg ccggaggttt ccatcggctt gaccggcttg ccggagccgc
      181 cgtcgtaggc gttcgggtcg aagcgcgcct tcagccactc gtagtcgacg ccccagacct
      241 tcgcccagtg cttccacgaa ccttcggcga ggccgtaata gccgggcagg ctgtcggcga
      301 gcacgccgaa gtcggtcgcg ccctggacgt tgtcatgacc gcggaagatg ttggtgccgc
      361 cgccggcctt gcccatgttg ccgagcgcga gctggaagat gcagtaggcg cgcgtgttgt
      421 tgttgccgat cgtgtgctgc gtaccgccca tgcaccatac gaaggtgccg gggcggtgct
      481 cggccatcag cttcgcgacc gtgtacgcat ccttctcctg gacgccggtg acgcgctcga
      541 cctcggccgg cggccacttc tcgacttcct tgcgcacctc gtccatgccg aacacgcgct
      601 gctgcaggaa gctgtcgtcc tgccagttgt tctggaacac gtgccagagc aggccccaga
      661 tgaacggcac gtcggtgccg gggcggatgc gcacgtagtg atcggcgtgc gcggcggtgc
      721 gcgtgaagcg cgggtcgatg acgatcagct tcgcgccgtc ctccttggcg cgcaggatgt
      781 gctgcatcga caccgggtgc gcctcggcgg cgttgctgcc gatcatcagg atggattttg
      841 cgtaggcgat gtcgttgtag gagttcgtca tcgcgccgta gccccaggtg ttggcaacgc
      901 cggcgaccgt ggtcgagtgg cagatgcgcg cctggtgatc gacgttgttc gtgccccaca
      961 tcgacgcgaa cttgcggaac agataggcct gctcgttgct gtgcttggcc gagccgagcc
     1021 agtagacggc gtcggggccg gattcctcgc ggatcttcag cagccgcgcc gacacctcgt
     1081 tcagcgccgt gtcccacgag atgcgctgcc actggccgtc gacgagcttc atcgggtact
     1141 tcaggcgccg ctcgccgtgg ccgtgctcgc ggatcgaggc gcccttcgcg cagtgcgcgc
     1201 ccatgttgaa cggatggtcg aaggccggct cctggccgac ccagacgccg ttctgcacct
     1261 cggcgtagat gccgcagccg accgagcagt gcgagcagat cgtgcgcttg atctcgaccg
     1321 ccgcgttgct cgcgtgggtg ccggcatcgg tcgcagccga ggcgcgacgc atcatcgtcg
     1381 gcccgagcgc ggccccgagc gcggtcgcac cgagcgcgag tcccgaggat ttgaggaagg
     1441 cgcgccggtc gacggtcgcg ccgaggaagg ccgcgaccgg cgaggtcgca gcggctgccg
     1501 gccgtgcccc ggcctgtttc ctgacgagtt tcatgcggac ggcctcagcg gcaggtgtcg
     1561 tagtaggcgc gtacgtgcgc ggtctcgcga tagccggcat cgagccgccc ggcttcttcg
     1621 ggttcgggcc gcacggcggc cggtgcggcg accgccacgc ccggcagcgc cagcgccacg
     1681 gccccggcgc cggcaccgct gccgaccagt ttcaggaaag cgcggcggtc gaggccggcg
     1741 gcaggggttt tctcgctcat gtcggtgctc ctgtgcgtcc gggcgcgtcg tgatgcgggt
     1801 tccgggagtt ccggtggttc tgctggcggt gccggctgac ttcagccggc cggcaccgac
     1861 gacggcgcga gcgccggcat cgcgccgtac tggcgttcga aggcaaggaa ggcttcggcg
     1921 aagcggccgg cggcgcggta gaagcgcgcc gattccgccg tctgcaggtc ggcaaacagt
     1981 cgcccggccc agggctcgat gtgatgcgcg aagaagcccg cggccgccgc cgagccctcg
     2041 ccgatcagca ggccgagcac ctcggcgagg gctgcgatgt ggtcctcggg ctcgcagatg
     2101 ccgggctggc gctcgaagcc gagccgggcg agatcagccc gcagttcgcc gagcggcttt
     2161 tccatcagga agccggtcag gtaccaggat gcgaacggca gcagctcgcc gcggccgatg
     2221 ccgatgaaca gcgcgtgata ttcgcgctcg agcgcgaccg gatcggccac cgcggccgcc
     2281 gcgcgcaagt cctgccaggc cgccgccagc gcgccgccct cgccggccgg cggcgtgtcg
     2341 atcgccgcca gttgcgcgag cagctccgcc gtcggcggcc ggcgcagcag tgctgcgagc
     2401 agcgcccagg tgccggcgcg caggcggtcg tcttcgtcgg gcgcggtcgt cggcgcggcg
     2461 tccatggtcg tcatggggta tttcctcggg cggtgctcgc ggaaagcaaa ccatgtgcca
     2521 tcaactttat cgttccatga cagacacttg tccgggcacc gccgcattct ggccgtgtga
     2581 ccggtcgcgc ccgggtgatc cggcgcgggt cagaatgacg cagccggcct gcgccaccgg
     2641 ccggtgcgcc ccgctacgct ctgcgccatc ccgtccgtct gcggatgtct gtggaaagcg
     2701 tgtcgatgcc gcccctgctg tctttcctgc tg
//
LOCUS       DAOQJH010000407         2728 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_26050_length_2728_cov_3.173588, whole genome shotgun sequence.
ACCESSION   DAOQJH010000407 DAOQJH010000000
VERSION     DAOQJH010000407.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2728)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2728)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2728
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26050_length_2728_cov_3.173588"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            309..2714
                     /gene="glgX"
                     /locus_tag="PLQ18_09515"
     CDS             309..2714
                     /gene="glgX"
                     /locus_tag="PLQ18_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015877603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HNE01933.1"
                     /translation="MPVADTLLPSSATALGALWDGRGAHFAVFAEDAEAVELCLFDAR
                     GEERRLQLPDCTNGIWRGYLPRVQPGQRYGYRVYGPYDPQRGHRFNPHKLLIDPYARQ
                     LAGALRWNKALYGYRMGGAKADLVPDRTDSARYLPKAVLVDASFDWGNDRAPRTPWSD
                     TVIYELHVKGFTQRFPGLGDTERGSFGALGDPAVIDYLRRLGVTAVELLPIHAFARDH
                     HLLDQGLTNYWGYNTLSYFAPEPAYLSDGTLGQIKWAVRELHAAGIEVILDVVYNHTC
                     EGSELGPTLSWRGFGNASYYRLLPDNARYHINDTGCGNTVNFSHPAVIRMVMDSLRYW
                     VQEFHVDGFRFDLGVTLGREVHGFDPGCGFFDALLQDPLLARVKLISEPWDIGPGGYQ
                     IGQHPAGMAEWNGRFRDDVRCYWRSDDGLRSALAARLQGSAELFDHHRRKPWASVNFI
                     TAHDGFTLRDLVSYNDKHNEANGEDNRDGGNDNASCNWGCEGPTDDVIVNDARLRVSR
                     SLLMTLFFSHGTPMLLAGDEFGQTQAGNNNVYCQDNELSWLDWERAATGEGRALRTFV
                     ERLIRLRRSWPLLQGLYFQHGHHESDAGLRDVFWFDERGVELIDSDWHNVAARLLGLR
                     RAVRRADGRLDVLVLLHNADGAGHTFRLPPPLYDFRVLIDSAHPELDSDPVRDGRYVV
                     SPNTCVLLAGHQRREPLPDEVAVESAALAAAGAAPVDAISDPPDGPIDPAPLPPPEPA
                     PDPAPEPWPEPVPDPVPEPRPQPVPDPAPEPPPRPWPDPAPEPQPEPLPGFDDPGPLP
                     VPA"
BASE COUNT          395 a         1030 c          915 g          388 t
ORIGIN      
        1 cacaccggcc gcaacggccg gcggccggac ctcgcgctga actgaggacg gcctgccgca
       61 ctccgttaat cctgccgggc aaaagccgct tttggttttt cgggttccgc cggcgcgccc
      121 ggtggatcgt gtgacccccc gcacacggtc cgccgggcgt gtccggtgcc tgtccgggct
      181 ggcagccggc ggcagccgcg aaggccggag tcgccgcttg gggaagtccg gtcaccgggc
      241 ggcagaaccc ggtccgacct gccaccgtgt accggccctg ccgacgatcc gccctgcacg
      301 aggacaccat gcctgtcgcc gacaccttgc tgccgagttc cgccaccgcc ctcggcgccc
      361 tctgggatgg gcgcggtgcg catttcgccg tgttcgccga ggatgccgag gccgtcgagc
      421 tgtgcctgtt cgatgcccgc ggcgaggaac gccggctgca gctgccggac tgcacgaacg
      481 gcatctggcg cggctacctg ccgcgcgtgc agcccggcca gcgctacggc taccgcgtgt
      541 acgggccgta cgacccgcag cgcggtcacc gcttcaatcc gcacaagctg ctgatcgacc
      601 cgtatgcgcg ccagctcgcc ggggcgctgc gctggaacaa ggccttgtac ggctaccgca
      661 tgggcggtgc gaaggccgat ctggtgccgg atcgcaccga cagcgcccgc tacctgccga
      721 aggcggtgct ggtcgatgcc tcgttcgact ggggcaacga ccgggcgccg cgcacgccgt
      781 ggtcggacac ggtgatctac gagctgcacg tcaagggctt cacgcagcgc tttcccggcc
      841 tgggcgacac cgagcgcggc agcttcggcg cgctcggcga tccggcggtc atcgactacc
      901 tgcgccggct cggcgtcacc gccgtcgagc tgctgccgat ccatgccttt gcccgcgatc
      961 accacctgct cgaccagggc ctgaccaact actggggcta caacacgctg tcgtacttcg
     1021 cgccggaacc ggcctacctg tccgacggca cgctcggcca gatcaagtgg gcggtccgcg
     1081 agctgcacgc cgccggcatc gaggtgatcc tcgatgtcgt ctacaaccac acctgcgagg
     1141 gcagcgaact cggtccgacg ctgagctggc gcggcttcgg caacgccagc tactaccggc
     1201 tgctgccgga caacgcccgc taccacatca acgacaccgg ctgcggcaac accgtcaact
     1261 tctcgcatcc ggcggtgatc cgcatggtca tggattcgct gcgctactgg gtgcaggaat
     1321 tccacgtcga cggcttccgc ttcgacctcg gcgtcacgct cgggcgcgag gtgcacggct
     1381 tcgaccccgg ctgcggcttc ttcgatgcgc tgctgcagga cccgctgctc gcccgcgtca
     1441 agctgatctc cgaaccctgg gacatcggcc ccggcggcta ccagatcggc cagcacccgg
     1501 ccggcatggc cgagtggaac gggcgcttcc gcgacgacgt gcgctgctac tggcgcagcg
     1561 acgacggcct gcgcagcgcg ctggccgcgc gcctgcaggg ctcggccgaa ctctttgacc
     1621 accaccggcg caagccctgg gcgagcgtga acttcatcac cgcccatgac ggtttcacgc
     1681 tgcgcgacct cgtcagctac aacgacaagc acaacgaggc caacggcgag gacaaccgcg
     1741 acggcggcaa cgacaacgcc agctgcaact ggggctgcga gggaccgacc gacgacgtga
     1801 tcgtcaacga cgcgcgcctg cgcgtcagcc gcagcctgct gatgacgctg ttcttcagcc
     1861 acggcacgcc gatgctgctc gccggcgacg agttcgggca gacgcaggcc ggcaacaaca
     1921 acgtgtactg ccaggacaac gagctgtcgt ggctcgactg ggagcgcgcc gcaaccggcg
     1981 agggcagggc gctgcgcacc ttcgtcgagc gcctgatccg gctgcggcgc agctggccgc
     2041 tgctgcaggg gctgtatttc cagcacggtc accacgagtc cgacgcgggc ctgcgcgacg
     2101 tgttctggtt cgacgagcgc ggcgtcgagc tgatcgacag cgactggcac aacgtcgccg
     2161 cgcgcctgct cggcctgcgc cgtgccgtcc gccgtgccga tggccggctc gacgtgctgg
     2221 tgctgctgca caacgccgac ggggccgggc acacgttccg gctgccgccg ccgctgtatg
     2281 acttccgcgt gctgatcgac agcgcccacc ccgaactcga cagcgatccg gtgcgggacg
     2341 gccgctacgt ggtctcgccg aacacctgcg tgctgctcgc cggccaccag cgccgggagc
     2401 cgctgcccga cgaggtggcg gtcgaatcgg ccgcgctcgc ggctgccggc gcagcgccgg
     2461 tcgatgcgat cagcgatccc ccggacggcc cgatcgatcc ggcgccgctg ccgccgcccg
     2521 agccggcgcc ggacccggcg cccgagccct ggcccgagcc cgtacccgat ccggtgcccg
     2581 aaccgcgtcc gcagccggtc cccgacccgg ctccggagcc cccgccgcgc ccgtggcccg
     2641 atccggcgcc cgagccgcaa cccgaaccgc tgccgggctt cgatgatccc gggccgctgc
     2701 cggtgccggc atgaacgccg gcgtgcag
//
LOCUS       DAOQJH010000408         2723 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_26141_length_2723_cov_4.414543, whole genome shotgun sequence.
ACCESSION   DAOQJH010000408 DAOQJH010000000
VERSION     DAOQJH010000408.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2723)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2723)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2723
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26141_length_2723_cov_4.414543"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..426
                     /gene="fliN"
                     /locus_tag="PLQ18_09520"
     CDS             <1..426
                     /gene="fliN"
                     /locus_tag="PLQ18_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HNE01934.1"
                     /translation="AAATEPAAASSAPPPKPAARPAAPPPKPAAATRNSGGFGTNFDT
                     AGDPVPSANGRAANLDVVLDIPITLSMELGRTRLSIRELLALSQGNVVRLDRPAGEPL
                     DILVNGCLVARGEVVVVNDRFGVRIVEIVSPEERIKRLR"
     gene            426..920
                     /gene="fliO"
                     /locus_tag="PLQ18_09525"
     CDS             426..920
                     /gene="fliO"
                     /locus_tag="PLQ18_09525"
                     /inference="COORDINATES: protein motif:HMM:NF016250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HNE01935.1"
                     /translation="MTETRHRTPRAAWRLPALASLFAAGRAAAAPETPPPAAPAFDVS
                     SLVNLGIKLLVVIAVILALAWLARQMPTLRRSSGPITILGGLNVGNRERIVLVEVHGE
                     RVLLGVTPGSVTTLHVLDRLAVHGAGAGETDGEDSPRRRFDFRSALADYHRAREDGGG
                     PPHP"
     gene            952..1674
                     /gene="fliP"
                     /locus_tag="PLQ18_09530"
     CDS             952..1674
                     /gene="fliP"
                     /locus_tag="PLQ18_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003296595.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HNE01936.1"
                     /translation="MCAATALALAEPRVPAVTMTPGTGGVQEWSVSLQILAIMTALTL
                     LPSLVLMTTAFTRIVIVLALLRQALGTMQTPSNQVLIGLALFLTVFIMGPVLQRMHDE
                     ALTPYLEERIGFQEAVEKARVPLSQFMLAQTRESDLALFAKLAGRESFESPESVPFTL
                     LVPAYVTSELKTAFQIGFMLFIPFLIIDFVVSSVLMAMGMMMLSPLVISFPFKLMLFV
                     LVDGWPLVMGTLAASFHGPGSG"
     gene            1697..1966
                     /gene="fliQ"
                     /locus_tag="PLQ18_09535"
     CDS             1697..1966
                     /gene="fliQ"
                     /locus_tag="PLQ18_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018229920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HNE01937.1"
                     /translation="MTPETVITLGQRTLEVSALLSAPLLGAMLIVGLVVGLFQAATQI
                     NEQTLSFVPKLIVAVLVIVAGGGWMIEILTDFTERLYRSIPTLIG"
     gene            1989..>2723
                     /gene="fliR"
                     /locus_tag="PLQ18_09540"
     CDS             1989..>2723
                     /gene="fliR"
                     /locus_tag="PLQ18_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020159728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HNE01938.1"
                     /translation="MPPLTSSEIAGWVGGFVWPLFRIAAMLSVAPVIGARSLPRRVRA
                     ALALVLTAVTVPLLPPGPQVEPMSPAGILIGVQQVLIGLYIGFALRLAFAALETGGQM
                     ISTQMGLGYASLIDPQNGASTPLLAQFYGLLGMLIFLGLDGHLLLIETVVESFHLLPV
                     STDGLALQRWRELADAGAMIFAGAVKFALPAMASLLVVNLAFGVMTRAAPQLNIFAVG
                     LPVTLLLGLAILLYSLPALAPEFRRLI"
BASE COUNT          343 a          950 c          963 g          467 t
ORIGIN      
        1 gccgccgcca cggaacctgc ggccgcgagc tccgccccgc ccccgaagcc ggcggcccgc
       61 ccggccgccc cgccgccgaa gccggctgcc gcaacccgca acagcggggg attcggcacg
      121 aatttcgaca cggccggcga cccggttccg tccgcgaacg ggcgggcggc caatctcgat
      181 gtcgtgctcg acatcccgat cacgctgtcg atggagctgg gccgcacgcg gctgtcgatc
      241 cgcgagctgc tggcgctcag tcagggcaac gtcgtgcgcc tcgaccgccc ggccggcgaa
      301 ccgctggaca tcctcgtcaa cggctgtctg gtggcgcgcg gcgaggtcgt cgtcgtcaac
      361 gaccgcttcg gcgtgcgcat cgtcgagatc gtcagtcccg aggaacgcat caagaggctg
      421 cgctgatgac ggaaacgcga caccggaccc cgcgggccgc gtggaggctg ccggcactgg
      481 ccagcctgtt cgctgccggg cgcgccgctg cagcccccga aaccccgccg ccggcggcgc
      541 cggccttcga cgtgagttcg ctggtcaacc tcggcatcaa gctgctggtc gtgatcgcgg
      601 tgatcctggc gctcgcgtgg ctggcgcggc agatgccgac gctcaggcgc agctccggac
      661 cgatcacgat cctcggcggg ctcaacgtcg gcaaccgcga gcgcatcgtg ctggtcgagg
      721 tgcacggcga gcgcgtgctg ctcggcgtga cccccggcag cgtcacgaca ctgcacgtgc
      781 tcgaccgcct ggccgtgcac ggcgcgggcg cgggcgagac ggacggcgag gacagcccgc
      841 gccggcgttt cgacttccgc tcggcgctcg ccgattacca ccgggcacgg gaggacggcg
      901 gtggaccgcc gcatccgtag ggcgctcgcg ctcggcggcg gactcgtcgc gatgtgcgcc
      961 gccacggcgc tcgcgctcgc cgagccgcgc gtgccggcgg tgacgatgac gccgggcacc
     1021 ggcggcgtgc aggagtggtc ggtcagcctg cagatcctcg cgatcatgac ggcgctgacg
     1081 ctgctgccgt cgctggtgct gatgaccacg gccttcacgc gcatcgtcat cgtgctggcg
     1141 ctgcttcgcc aggcgctcgg cacgatgcag acgccgtcga accaggtgct gatcgggctg
     1201 gcgctgttcc tgaccgtgtt catcatgggg ccggtgctgc agcgcatgca cgacgaggcg
     1261 ctgacgccgt atctcgaaga gcgcatcggc tttcaggaag cggtcgagaa agcgcgcgtg
     1321 ccgctgtcgc agttcatgct cgcgcagacg cgcgagtcgg atctggcgct gttcgcgaag
     1381 ctcgccgggc gcgagtcctt cgaatcgccc gagagcgtgc cgttcacgct gctggtgccg
     1441 gcctacgtca ccagcgagct gaagaccgcc ttccagatcg gcttcatgct gttcatcccg
     1501 ttcctgatca tcgacttcgt ggtctccagc gtgctgatgg cgatgggcat gatgatgctg
     1561 tcgccgctgg tgatctcctt tccgttcaag ctgatgctgt tcgtgctggt cgacggctgg
     1621 ccgctggtca tgggcacgct ggccgcgagc ttccacggac cgggcagcgg ctgaacgcac
     1681 gcgaggcctt gctgccatga ctccggaaac cgtgatcacg ctcggccagc gcacgctgga
     1741 ggtctcggcg ctgctgtcgg cgccgctgct cggggcgatg ctgatcgtcg gcctggtcgt
     1801 cggcctgttc caggcggcga cgcagatcaa cgagcagacg ctgtccttcg tgccgaagct
     1861 gatcgttgcg gtgctggtca tcgtcgccgg cggcggctgg atgatcgaga tcctgaccga
     1921 cttcaccgag cgcctgtacc gcagcatccc gacgctgatc ggctgatgcg cgtgccggat
     1981 tcgccccgat gccgccgctg acgagcagcg agatcgccgg ctgggtcggc ggtttcgtgt
     2041 ggccgctgtt ccggatcgcc gcgatgctgt cggtcgcgcc ggtgatcggt gcgcgctcgc
     2101 tgccgcggcg cgtgcgcgcg gcactggcgc tggtgctgac ggcggtgacc gtgccgctgc
     2161 tgccgccggg gccgcaggtc gagccgatgt cgccggccgg catcctgatc ggtgtgcagc
     2221 aggtgctgat cggcctgtac atcggctttg cactgcgcct ggcgttcgcg gcgctcgaaa
     2281 ccggcgggca gatgatctcg acgcagatgg ggcttggcta cgcctcgctg atcgatccgc
     2341 agaacggtgc ctcgacgccg ctgctcgcgc agttctacgg cctgctcggc atgctgatct
     2401 tcctcggcct cgacggtcac ctgctgctga tcgagaccgt ggtcgagagc ttccacctgc
     2461 tgccggtgtc gaccgacggc ctggccctgc agcgctggcg cgagctcgcc gatgccggcg
     2521 cgatgatctt cgccggggcc gtgaagttcg cgctgccggc gatggcctcg ctgctggtcg
     2581 tcaacctcgc cttcggcgtc atgacccgcg ccgcgccgca gctgaacatc ttcgcggtcg
     2641 ggctgccggt gacgctgctg ctcgggctgg cgatcctgct gtattcgctg ccggcgctcg
     2701 cgccggaatt ccgtcgcctg atc
//
LOCUS       DAOQJH010000409         2722 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_26163_length_2722_cov_3.610424, whole genome shotgun sequence.
ACCESSION   DAOQJH010000409 DAOQJH010000000
VERSION     DAOQJH010000409.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2722)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2722)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2722
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26163_length_2722_cov_3.610424"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..118)
                     /locus_tag="PLQ18_09545"
     CDS             complement(<1..118)
                     /locus_tag="PLQ18_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014948618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HNE01939.1"
                     /translation="MFDGKSILITGGTGSFGRTFARTLLARHQPERVIVYSRD"
     gene            complement(181..1395)
                     /gene="flgL"
                     /locus_tag="PLQ18_09550"
     CDS             complement(181..1395)
                     /gene="flgL"
                     /locus_tag="PLQ18_09550"
                     /inference="COORDINATES: protein motif:HMM:TIGR02550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HNE01940.1"
                     /translation="MIRISTSYTQEQGVQYMRDAQKRVVDTQAELATGKRISSPSDDP
                     VGAARLIDLHHQIDSTARFQTNIAMATGRLATSDSILDEAGQLTQRARELVVQANNAP
                     LADSDRVSIAQELRQIRGQLLEHANSKDGNGDYLYAGYRTQTAPFIETTGGQVVYQGD
                     SGQRLLGIAPTRSVAVSDAGDSVFMNIRAGNGVFATTADAGNSGSAVIEPGDVLDPTA
                     FEHHRFQIDFQSSTDFTITDLSEVPPTGPGPVQTLPADGRIEFNGISVKLTGAPANGD
                     RFEVAPAPYQDAFTTLDKVITALETPVAATGAARTKFAQTMADAMQNLDQMHSNFLDT
                     RTQIGARLNGLETQESANSEFSLRMQSIANDIEGLDYAEASSRLAAYTLTLQAAQQSY
                     VKIQNLNLFNYL"
     gene            complement(1683..>2722)
                     /locus_tag="PLQ18_09555"
     CDS             complement(1683..>2722)
                     /locus_tag="PLQ18_09555"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HNE01941.1"
                     /translation="KEVEATTPVLAPVSVPAPAAPAAATTAAGGETAATPAQPAAAEP
                     APVETVLAEVYDATLVEAVHRFQARHGLTEDGVIGAATVAAMNVSVRERIAQVRLNLE
                     RWRWLPGDLGQRYIVVNIPGFELQVIERGRPVLEAKVVVGTQVKPTPVFTGRMQQVVF
                     APYWNVPGTIASEELLPKLRKNPGEFVKTNMKVITGSGAVVDPTAVNWGEYHPGNFPF
                     RLRQEPGAANPLGRVKFIFPNRYDVYLHDTSKPALFERSQRTYSHGCIRISRPAELAE
                     YLLKSDPRWNRAAIDAAMDAGRERAVPVPEPLPVHLLYWTSWVDAQGLLQFRADVYGR
                     DLALRKALSAL"
BASE COUNT          384 a          894 c          973 g          471 t
ORIGIN      
        1 catcgcggga atagacgatc acgcgctcgg gctggtggcg cgccagcagc gtgcgcgcga
       61 aggtccggcc gaaggagccg gtaccgccgg tgatcaggat gctcttgcca tcgaacatgg
      121 cgtgcctcgg cttgcagcgc actgcagtca gtttcggagg atgacgggtg acggaggtga
      181 ttacaggtaa ttgaacaggt tcaggttctg gatcttcacg taggactgct gcgcggcctg
      241 cagcgtcagc gtgtaggccg cgagccggct gctcgcctcg gcgtagtcca gcccttcgat
      301 gtcgttggcg atcgactgca tgcgcaggct gaattcgctg ttcgcggatt cctgggtttc
      361 gaggccgttg aggcgggcgc cgatctgcgt gcgcgtgtcg aggaagttcg aatgcatctg
      421 gtcgaggttc tgcatcgcgt cggccatggt ctgcgcgaac ttcgtgcgcg ccgcgccggt
      481 ggcggcgacc ggggtttcca gcgcggtgat gaccttgtcg agcgtcgtga acgcgtcctg
      541 gtacggcgcc ggtgcgacct cgaagcggtc gccgttcgcc ggcgcgccgg tgagcttcac
      601 cgagatgccg ttgaactcga tgcgaccgtc ggccggcagc gtctgcaccg gccccggccc
      661 ggtcggcggc acctcgctca ggtcggtgat cgtgaaatcg gtcgaggact ggaaatcgat
      721 ctggaagcgg tggtgctcga acgcggtcgg gtcgagcaca tcgccgggtt cgatgaccgc
      781 cgagccgctg ttgccggcat cggccgtggt cgcgaacacg ccgttgccgg cgcggatgtt
      841 catgaacacg ctgtcgccgg catcggagac cgccaccgag cgcgtcggcg cgatgccgag
      901 caggcgctgg ccgctgtcgc cctgatagac gacctggccg ccggtggtct cgatgaacgg
      961 cgcggtctgg gtcctgtagc cggcgtacag gtagtcgccg ttaccgtcct tgctgttggc
     1021 gtgttcgagc agctggccgc ggatctggcg cagctcctgc gcgatgctga cgcggtcgct
     1081 gtcggcgagc ggtgcgttgt tggcctgcac gaccagttcg cgcgcgcgct gcgtcagctg
     1141 cccggcctca tcgaggatgc tgtcgctggt cgcgagccgc ccggtcgcca tcgcgatgtt
     1201 ggtctggaag cgcgcggtgc tgtcgatctg gtgatgcagg tcgatcaggc gcgccgcgcc
     1261 gaccggatca tcggacggcg acgagatgcg cttgccggtc gccagttcgg cctgggtgtc
     1321 gacgacgcgc ttctgcgcat cgcgcatgta ctgcacgccc tgttcctggg tgtacgacgt
     1381 cgaaatgcgg atcatggcgc gctcctcgta ctgccgaaga tgcagaagac ggcaggcgcg
     1441 gccacacgcc gatccgcgca ccggccgcac ggagcggacg gttcggggca tcgcaacgga
     1501 gtgctggaca tggcgcgcaa cctgcgggga cggacggtga gccgtgcttc agcaggattc
     1561 gcgccagatc attaattcat tgtcatgaaa gcagaatccg gccttgcgcc ggattcttga
     1621 cgccgtcacc ggcgcggaaa tccgtcgccg gccgtttgcc ggcgacgaca ggccggcgcg
     1681 cgctacagcg ccgacagcgc cttgcgcagc gccagatcgc gtccgtagac atcggcccgg
     1741 aactgcagca ggccctgcgc atcgacccag ctcgtccagt acagcagatg caccggcagc
     1801 ggctccggca ccggcaccgc acgctcgcgc ccggcatcca tcgccgcatc gatcgcggcg
     1861 cggttccagc gcggatcgct cttcagcagg tattcggcca gctccgcggg cctggagatg
     1921 cggatgcagc cgtggctgta ggtgcgctgg ctgcgctcga acagcgccgg cttcgaggtg
     1981 tcgtgcaggt agacgtcgta gcggttcggg aagatgaact tcacgcgccc gagcggattg
     2041 gccgcgcccg gctcctgacg cagccggaac gggaagttgc cggggtggta ctcgccccag
     2101 ttcacggcgg tcggatcgac cacggcgccg ctgccggtga tgaccttcat gttggtcttc
     2161 acgaactcgc cgggattctt gcgcagcttc ggcagcagct cctcggaggc gatcgtgccc
     2221 ggcacgttcc agtacggcgc gaacacgacc tgctgcatgc gcccggtgaa caccggcgtc
     2281 ggcttgacct gcgtgccgac cacgaccttc gcttcgagca ccggcctgcc gcgctcgatc
     2341 acctgcagct cgaagcccgg gatgttgacg acgatgtagc gctggccgag atcgccgggc
     2401 agccagcgcc agcgttcgag gttcagccgc acctgtgcga tgcgctcgcg cacgctgacg
     2461 ttcatcgccg cgaccgtcgc cgcgccgatc acgccgtcct cggtcaggcc gtggcgggcc
     2521 tggaagcggt gcacggcctc gaccagcgtg gcgtcgtaga cctcggcgag cacggtctcg
     2581 accggcgccg gctccgcggc cgccggctgc gcgggcgtgg cggcggtctc gccgccggct
     2641 gccgtcgtgg ccgctgcggg tgcagccgga gccggcacgc tgaccggagc cagcaccggc
     2701 gtcgtcgcct cgacttcctt gg
//
LOCUS       DAOQJH010000410         2721 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_26176_length_2721_cov_3.017629, whole genome shotgun sequence.
ACCESSION   DAOQJH010000410 DAOQJH010000000
VERSION     DAOQJH010000410.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2721
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26176_length_2721_cov_3.017629"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            17..700
                     /locus_tag="PLQ18_09560"
     CDS             17..700
                     /locus_tag="PLQ18_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006747307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNE01942.1"
                     /translation="MIVRQMKLGPMDNFVYLIADRASARAAVVDPAWDVPAVLARAAG
                     LGLHITDALLTHSHQDHINGVGALLEATDARVHLHRDEAAFWGAELVRPALHRGGDTL
                     LLGSTPIEVLHTPGHTPGSVCYRVHDEVVTGDTLFVYGCGRCDLRGGDPNLMHDSLCR
                     LCRELPGGTLIRPGHDYGITPVSTMLEQMQGNPFLHFHEDQRAGFVEYRMHLHDREEP
                     YGPEPAPQG"
     gene            complement(758..2392)
                     /locus_tag="PLQ18_09565"
     CDS             complement(758..2392)
                     /locus_tag="PLQ18_09565"
                     /inference="COORDINATES: protein motif:HMM:NF012267.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNE01943.1"
                     /translation="MPDPDPSSAITSADARAQALAAAAAVAVTPTSTVDFVSQGRVLI
                     LGPGTAALTAAGRLCTTLQVVVLASGPAAVPVPAGVTLLYREQRRLTLDGQLGGFFAT
                     LDANGRSFDLGERIDAQRPVFDLVLDLGDEPAFRREWGPLGYYRPLDAEALEAALAAL
                     PDLCGEFEKPKFFAYDASICAHGQAGLDGCRRCIEACPAEAIESLLDRVRVNPNLCQG
                     GGACASACPSGAIVYRYPPAADTLLRLRKLLTTYRRAAGGERPVLVFMDEHAGIDPLA
                     WPGRVLPVLLAETASAGMDLWLGVLAYGAAAVRVLRAPDLAPKLERALDEQLGFARAI
                     LAGLGWPADALGWLAVDGDPLTGAEMPAMPAAGYAGSEAKRERLWAAIDHLHAHAPQP
                     AEVLALPQGAPFGQILVNPETCTLCMACVSVCAPKALADGGEEPKLRFVEAACVQCGL
                     CETACPEHAIERETRLLVPFEQRRRQRTLHEDQPHHCPQCGKAFATQSMIRTITGRLA
                     HHPMFAGDGLRRLLLCEDCRVRDLYRSEGLGQRPGP"
     gene            complement(2385..>2721)
                     /locus_tag="PLQ18_09570"
     CDS             complement(2385..>2721)
                     /locus_tag="PLQ18_09570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3306 domain-containing protein"
                     /protein_id="HNE01944.1"
                     /translation="RTQALRKLFHQPEYNVIDPLNDYIDDFTDFAPLGDVMTADLRHR
                     LEQEAEAVAQRLQVEERPPAGAAPSAVPADAAPAAVVPAVTDAPPAGEKLISSSPEAD
                     VPPESPSRA"
BASE COUNT          420 a          990 c          940 g          371 t
ORIGIN      
        1 cgaaggagtc cgacaaatga tcgtgcgcca gatgaagctc gggccgatgg acaacttcgt
       61 ctacctgatc gcggatcgcg ccagcgcccg cgccgccgtc gtcgatccgg cctgggacgt
      121 gccggccgtg ctggcgcgcg cggccggact cggcctgcac atcaccgacg cgctgctgac
      181 gcacagccac caggatcaca tcaacggcgt cggagcgctg ctcgaagcca ccgacgcccg
      241 ggtgcatctg catcgcgacg aggcggcgtt ctggggtgcg gagctcgtgc gtccggccct
      301 gcaccgcggc ggcgacacgc tgctgctcgg ctccaccccg atcgaggtgc tgcacacccc
      361 cggccacacg cccggttcgg tctgctaccg cgtccatgac gaggtcgtca ccggcgacac
      421 gctgttcgtg tacggctgcg gacgctgcga cctgcgcggc ggcgacccga acctcatgca
      481 cgattcgctc tgccggctct gccgcgaact gcccggcggc acgctgatcc gccccggcca
      541 tgattacggc atcacgccgg tctcgacgat gctcgaacag atgcagggca atcccttcct
      601 gcattttcat gaggatcagc gcgccggttt cgtcgaatac cgcatgcacc tgcacgatcg
      661 cgaggagccc tacgggccgg aaccggcgcc gcaaggctga aggcttgcgg ctgcacgcaa
      721 acgcccggcc ggcaggctgc gcctgcggtg cctgccctca gggtcccggc cgctgcccca
      781 gtccctcgct gcggtacagg tcgcgcacgc ggcagtcctc gcagagcagc agccggcgca
      841 ggccgtcgcc ggcgaacatc gggtgatggg cgagccgccc ggtgatcgtg cggatcatcg
      901 attgcgtcgc gaaggccttg ccgcattgcg gacagtggtg cggctgatcc tcatgcagcg
      961 tgcgctgacg gcggcgctgt tcaaacggca ccagcagccg ggtctcgcgc tcgatcgcat
     1021 gctccggaca ggcggtctcg cacagcccgc actgcacgca ggccgcctcg acgaagcgca
     1081 gcttcggttc ctccccgcca tcggcgagcg ccttcggcgc gcacaccgac acacaggcca
     1141 tgcacagcgt gcaggtttcg ggattgacga ggatctgccc gaacggtgcg ccctgcggca
     1201 gggccagcac ctcggccggc tgcggcgcgt gcgcatgcag atgatcgatc gccgcccaca
     1261 accgttcgcg cttggcctcg ctgccggcgt agcccgccgc cggcatggcc ggcatttcgg
     1321 cacctgtcag cggatcgccg tccacggcca gccagccgag tgcatcggcc ggccagccga
     1381 gtccggcgag gatcgcgcgc gcaaacccga gctgctcatc gagcgcccgt tcgagcttcg
     1441 gcgccagatc cggtgcgcgc agcacgcgca ccgctgccgc gccatacgcc agcaccccga
     1501 gccagaggtc catgccggcc gaggcggtct cggcgagcag caccggcagc acgcgccccg
     1561 gccaggccag cggatcgatg ccggcgtgct cgtccatgaa caccagcacc ggccgctcgc
     1621 cgccggcggc gcggcggtac gtcgtcagca gcttgcgcag gcgcagcagc gtgtcggccg
     1681 ccggcggata gcggtagacg atcgcaccgc tcggacaggc gctcgcgcag gcgccaccgc
     1741 cctgacagag gttcggattg acgcgcacgc gatcgagcag cgattcgatc gcctcagccg
     1801 ggcaggcttc gatgcagcgc cggcagccat cgagtccggc ctggccgtgc gcgcagatcg
     1861 aggcgtcgta ggcgaagaac ttcggcttct cgaactcgcc gcacaggtcc ggcagcgcgg
     1921 cgagcgcggc ttcgagggct tcggcgtcca gtggccggta atagccgagc ggtccccatt
     1981 cgcgcctgaa cgccggctcg tcgccgagat cgagcacgag atcgaacacc ggccgctgcg
     2041 catcgatgcg ttcgccgaga tcgaacgaac gcccgttcgc atcgagcgtc gcgaagaagc
     2101 cgccgagctg gccatcgagc gtcagccggc gctgctcgcg atacagcagc gtcacaccgg
     2161 ccggcaccgg cacggcggcc ggcccggagg cgagcacgac gacctgcagc gtcgtgcaga
     2221 gccgcccggc cgccgtcagc gccgccgtac ccggcccgag gatcagcacc cggccctgcg
     2281 agacgaaatc gacggtcgag gtcggcgtca ccgccacggc ggcggccgcg gccagcgcct
     2341 gcgcgcgcgc atccgcgctc gtgatcgcgg aggaaggatc gggatcaggc acgggaaggg
     2401 ctctccggcg gcacatcggc ctcaggactt gaggaaatca gcttctctcc ggcgggcgga
     2461 gcatcggtca cggccggcac gaccgctgcc ggcgcggcat cggcagggac cgcggacggg
     2521 gccgcgccgg ccgggggccg ttcttcgacc tgcagccgct gcgcgacagc ctcggcctcc
     2581 tgttcgagcc gatggcgcag gtcggcggtc atcacgtcac cgagcggcgc gaagtcggtg
     2641 aagtcatcga tgtaatcgtt gagcggatcg atgacgttgt attcgggctg atgaaacagc
     2701 ttgcgcaggg cctgcgtgcg c
//
LOCUS       DAOQJH010000411         2718 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_26219_length_2718_cov_5.243710, whole genome shotgun sequence.
ACCESSION   DAOQJH010000411 DAOQJH010000000
VERSION     DAOQJH010000411.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2718)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2718)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2718
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26219_length_2718_cov_5.243710"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /gene="ccsA"
                     /locus_tag="PLQ18_09575"
     CDS             <1..183
                     /gene="ccsA"
                     /locus_tag="PLQ18_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019595160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HNE01945.1"
                     /translation="KETWALIVWLNYAAWLHLRLTKGLRGPPMAWWAIIGLFVTAFAF
                     LGVNMWLSGLHSYGTL"
     gene            234..902
                     /locus_tag="PLQ18_09580"
     CDS             234..902
                     /locus_tag="PLQ18_09580"
                     /inference="COORDINATES: protein motif:HMM:NF013488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HNE01946.1"
                     /translation="MPKFPLIPAVLLAFACALLPATASADEAPAFKEGTEYTVLKTPV
                     ATSTAEQPGKVEVVELFWFGCPHCYRLEPAIQEWLKARPEEAVFVRVPAALAPNWELG
                     ARAYYAALSLDVEDKLHQPLFDAIHKDRKLALITDENALVSFVSGLGIDAEAYRKALH
                     SFDVETRLRKSKQLVVQYRVTGVPAVIVAGKYVTDIAMAGSPARVMDVVNFLVRKESG
                     KSGS"
     gene            989..1804
                     /locus_tag="PLQ18_09585"
     CDS             989..1804
                     /locus_tag="PLQ18_09585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011630582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HNE01947.1"
                     /translation="MERATEPALQRLRLLSYNIQSGLSTRRYSDYVTHSWRHVLPVPS
                     RMVNLDAIADLAAGYDIVGLQEVDPGSLRSGFVDQAAYLAARGGFPFVHDQSNRRIGM
                     ISHHANAVLSRHRPSAIEDRKLPGLIKGRGVLCVRYGDGPEALHLFIIHLALGRRGRM
                     QQLGFLAELIADARHAIVMGDLNCHAGSPEIAHLLARTSLAEPEALHLPTFPSWAPNR
                     QLDHILVSESIRVERVEVLRHTYSDHLPIAMEVVLPAAAWWSGIAPAAAASLP"
     gene            complement(1717..2532)
                     /locus_tag="PLQ18_09590"
     CDS             complement(1717..2532)
                     /locus_tag="PLQ18_09590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE01948.1"
                     /translation="MRLPVPVPVLRRGLALLLLAAAAPPSPAAEAVETFTGIARLADG
                     SVYYVERHRVEWRGERPVTSFTRYSDAAGQSFGEMDSRFPAPTPAPYTPAYRFEDRRL
                     GRSDQLQIHGDRAETRAIAGDGSPEKSGNYALAPDLVSGQGLHFLLRARLAAFVDDPG
                     FELHARLLIPLDAAIYPLRVRRLALDDGRLTLRIDIDHWLYRLFAPHMDIAYDVRSRR
                     LLWYEGPTHILDALRAVRTVRIDYRYGSEAAAAGAIPDHHAAAGSTTSIAIGR"
BASE COUNT          410 a          949 c          952 g          407 t
ORIGIN      
        1 aaggaaacct gggcgctgat cgtctggctg aactacgccg cctggctgca cctgcgcctg
       61 accaagggcc tgcgcggccc gccgatggcg tggtgggcga tcatcggcct gttcgtgacc
      121 gcgttcgcgt tcctcggcgt caacatgtgg ctgtcgggcc tgcattccta cggcacgctg
      181 tgagcgcgcc cggcgccgaa ccgtcttccc cccgtaccga aggaatcgtc cgcatgccga
      241 agtttccgct gatccccgcc gtgctgctgg ccttcgcctg cgcgctgctg ccggccaccg
      301 cttccgccga cgaggccccg gccttcaagg aaggcaccga gtacacggtg ctgaagacgc
      361 cggtcgccac gtcgacggcc gaacagcccg gcaaggtcga ggtcgtcgag ctgttctggt
      421 tcggctgccc gcactgctac cggctggagc cggcgatcca ggaatggctg aaggcgaggc
      481 ccgaggaggc cgtgttcgtg cgcgtgccgg cggcgctcgc gccgaactgg gaactcggcg
      541 cgcgcgccta ctacgcggcg ctgtcgctcg atgtcgagga caagctgcac cagccgctgt
      601 tcgatgcgat ccacaaggac cgcaagctgg cgctgatcac cgacgagaac gcgctggtca
      661 gcttcgtcag cggcctcggc atcgacgccg aggcctaccg caaggcgctg cattcgttcg
      721 atgtcgagac gcggctgcgc aagtccaagc agctcgtcgt gcagtaccgc gtcaccggcg
      781 tgccggccgt catcgtcgcc ggcaagtacg tcaccgacat cgccatggcc ggatcgccgg
      841 cgcgggtgat ggacgtcgtg aacttcctcg tgcgcaagga aagcggcaag agcggcagct
      901 agttgatgag ccgcatgagc cataactgag cgggtcgcgc tgcgcggcgc cggtggcggc
      961 gccgcccggc atcgccggga gccatgtcat ggagcgagcc accgagcccg cactgcagcg
     1021 cctgcggctg ctcagctaca acatccagtc cggcctgagc acgcgccggt attcggatta
     1081 cgtcacgcac agctggcgcc acgtgctgcc ggtgccgtca cggatggtca acctcgacgc
     1141 catcgccgat ctcgccgccg gctacgacat cgtcggcctg caggaggtcg accccggcag
     1201 cctgcgcagc ggcttcgtcg atcaggccgc gtatctggcc gcgcgcggcg gcttcccgtt
     1261 cgttcacgac cagtcgaacc ggcgcatcgg catgatctcg catcacgcca acgccgtgct
     1321 gtcgcgccac cgcccgagcg cgatcgagga ccgcaagctc cccggcctga tcaagggccg
     1381 cggcgtgctg tgcgtgcgct acggcgacgg ccccgaggcg ctgcacctgt tcatcatcca
     1441 cctcgcgctc ggccggcgcg ggcgcatgca gcagctcggc tttttggccg aactgatcgc
     1501 cgatgcccgc catgccatcg tcatgggcga cctgaactgt cacgccggca gcccggaaat
     1561 tgcccatctg ctggcgcgca cctcgctggc cgagcccgag gcgctgcacc tgccgacctt
     1621 cccgagctgg gcgccgaacc gccagctcga ccacatcctg gtgtcggagt cgatccgcgt
     1681 cgaacgcgtc gaggtgctgc gccacaccta ttcggatcac ctgccgatcg cgatggaggt
     1741 cgtgctgccg gccgcggcgt ggtggtccgg gatcgcgccg gccgcggcgg cctcgctgcc
     1801 gtagcggtag tcgatgcgca ccgtgcgcac cgcgcgcagt gcatcgagga tgtgcgtcgg
     1861 tccctcgtac cagagcaggc gacggctgcg cacgtcgtag gcgatgtcca tgtggggcgc
     1921 gaacagccgg tacagccagt gatcgatgtc gatgcgcagc gtcagccgcc cgtcgtcgag
     1981 cgcgagccgg cgcacgcgca gcgggtagat cgccgcatcg agcggtatca gcagccgcgc
     2041 atgcagctcg aagcccggat cgtcgacgaa ggccgcgagc cgcgcgcgca gcaggaagtg
     2101 caggccctgg ccgctgacca gatccggcgc aagcgcatag ttgccgcttt tctccggact
     2161 gccgtcaccg gcgatggcgc gggtctcggc ccggtcgccg tggatctgca gctgatcgct
     2221 gcgcccgagc cggcggtcct cgaagcgata cgccggcgtg tatggcgccg gggtcggcgc
     2281 cggaaagcgc gaatccatct cgccgaagga ctggccggcg gcatcgctgt agcgggtgaa
     2341 gctggtgacc ggccgttctc cgcgccattc gacccgatga cgctcgacgt aatacacgct
     2401 gccatcggcc agccgggcga tgccggtgaa ggtttcgacc gcttcggcgg ccggggacgg
     2461 cggcgcagcg gcggccagca gcagcagggc aagaccacga cgcaggacgg ggacagggac
     2521 gggcaggcgc atgatccggg ctccggcggc gggcaggggg attgccgcca cccgggatgc
     2581 gcgggcctgc cgaagatgga tgcacacgga tgctgccggc gtggctgcgc acgaggtgcg
     2641 ggccggcacg caggcagtgc gtcggctatg ctgcgccggc cgtgaaacac acggcgcacc
     2701 gttgttgctg cgccggac
//
LOCUS       DAOQJH010000412         2718 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_26229_length_2718_cov_2.938415, whole genome shotgun sequence.
ACCESSION   DAOQJH010000412 DAOQJH010000000
VERSION     DAOQJH010000412.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2718)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2718)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2718
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26229_length_2718_cov_2.938415"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..411
                     /gene="gspM"
                     /locus_tag="PLQ18_09595"
     CDS             <1..411
                     /gene="gspM"
                     /locus_tag="PLQ18_09595"
                     /inference="COORDINATES: protein motif:HMM:NF016493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HNE01949.1"
                     /translation="AALYALAWAPLAAENAARRQRIQALQADLAWMQQMAPRLQQAGA
                     RTSPVEDGNAQAPLPSRLETSLQRAGLGAGVRSIEASSDARVTIAFESVAFDALIGWL
                     EALQREQGITVGQAVLERLPAPGQVRARLEFGGG"
     gene            568..1212
                     /locus_tag="PLQ18_09600"
     CDS             568..1212
                     /locus_tag="PLQ18_09600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE01950.1"
                     /translation="MFAAGGGFHVCNHRGTENVIDVEPYGCRSDRRGGVRRPYRNGSG
                     PALAPARPGPTGAALDEMAALLDHLQRVAHPRRAPVLDALDRRYAQRWLAYADLLETQ
                     LAVQEAGVPPSLLERAHVPVILAGVRDGSLQTQGDVQQHCGLKEANASRVLALLDAAE
                     LVLRQRHGREKRLVAGPRLPEALQLLEDAGRLPLATTPARTATSRRGSSFFKAA"
     gene            1216..>2718
                     /locus_tag="PLQ18_09605"
     CDS             1216..>2718
                     /locus_tag="PLQ18_09605"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HNE01951.1"
                     /translation="MQTHITTFYSFKGGVGRSLLLANVGALLAQQAAPVLLWDLDLEA
                     PGLHHIPALRPKPVPKAGFLEWLQGRGERLGQSPSAAELNRLWKSVKPVPGREGLFIL
                     PACGDKADFAGLYQQIAWQGPMVEQPEQGLNLFRSLLAHLAEKGGFAHLLLDARTGVT
                     DLGGLMAAVLPHATVLVGGYGRQNRAGLLQVYRALQPAVDDRLPERGSLPPLARLLVT
                     SPVPDLPERRDECRRSWREDFVLAPEATQFEIPFDGRLLFEERLLAIEEPASPAGLAY
                     RQIADALAGLRDENQRTQKQASIAADIHDDDPRRTGAERVVRGKRFEERVARLLTLLD
                     YRVEPEQYLDNANKVDLIARRTAGLRQECFLVECKDWEKPVPKAELERFRSWLNGPQA
                     QQMRAEGLYVARSYTRDALSYAKSQGLIAVTPQELEAQLFDFRPYLDRLRRRFEESPL
                     ARHYVAQRVLLESEPELKDGVDLIEHAQAWANGAGRRLWLVLGDYGTGKSS"
BASE COUNT          421 a          842 c         1003 g          452 t
ORIGIN      
        1 gcggcgctgt atgccctggc ctgggctccg cttgcagccg aaaacgccgc ccggcgccag
       61 cgcatccagg cattgcaggc cgatctggcg tggatgcagc agatggcccc gcggctgcag
      121 caggccggtg cgcgaacatc cccggtcgaa gacggcaacg cccaggcccc cctgccgagc
      181 cgcctggaaa ccagcctgca gcgtgccggc ctcggcgccg gggtccgcag catcgaggcg
      241 agcagtgatg cgcgtgtcac gatcgcgttc gaatccgtcg ccttcgatgc gctgatcggc
      301 tggctcgaag ccttgcagcg cgagcagggg attaccgtcg ggcaggcggt actggagcgt
      361 ttgccggccc cggggcaggt gcgggcgcgg cttgaattcg ggggtggatg agagggcagg
      421 cccgtccgtc tgtctcgccg gcgatcgagt tcttgttccc gtctgccggt caggcacagg
      481 ccgggtcatg gcatcaacgc gatgaagcgc gccggcggca acccgctctt cgggtaccag
      541 agggcgacgg ggccgagagt caggcggatg ttcgccgccg gcggcggatt tcatgtgtgt
      601 aaccatcgag gcacggagaa cgtgattgat gttgagccgt acggttgtcg atcagatcgt
      661 cgtggcggcg tgcgccggcc ataccgcaat ggctctggtc cggctctggc accagctcga
      721 ccgggaccga ccggcgccgc gctcgacgag atggcggcgc tgctcgatca cctgcagcgg
      781 gtggcgcacc cgcggcgtgc gccggtgctc gatgcgctgg accggcgcta tgcgcagcgc
      841 tggctggcct atgccgatct gctcgaaacc cagcttgcgg tgcaggaagc cggtgtgccg
      901 cccagcctgc tggagcgcgc gcatgtgccg gtgatcctcg cgggcgtgcg cgacggcagt
      961 ctgcaaaccc agggcgatgt gcagcagcac tgcggcttga aggaggccaa tgccagccgt
     1021 gtgctggcgc tgctcgacgc cgccgaactg gtgctgcgcc agcggcacgg gcgtgagaag
     1081 cggttggtgg ccgggccgcg tctgcctgaa gccttgcagt tgctggagga tgccggccgg
     1141 ctgccacttg caacgacacc ggcacgcaca gcgacttcac ggcgcggcag ttcattcttc
     1201 aaggcggcct gagcggtgca gacccacatc acgaccttct acagcttcaa gggcggcgtc
     1261 ggtcgcagtc tgctgctggc caacgtcggc gcgctgctcg cacaacaggc agcgccggtg
     1321 ctgttgtggg atctggacct cgaagcgccg ggcctgcatc acattccggc gctgcgtccg
     1381 aaaccggtgc cgaaagcggg gtttctggaa tggctgcagg ggcgcggtga gcggctcggt
     1441 caaagcccgt ccgcggccga gctgaaccgg ctgtggaaga gcgtgaaacc cgtgccgggg
     1501 cgggaggggc tgttcatcct gccggcctgt ggcgacaagg cggatttcgc cgggctgtat
     1561 cagcagatcg catggcaggg gccgatggtc gagcagcccg agcaagggct gaacctgttc
     1621 cggtccctgc tcgcgcatct ggcggagaag ggcggtttcg cgcatctgct gctcgatgcc
     1681 cgtaccggtg tgaccgatct cggcgggctg atggccgcgg tgctgccgca tgcgacggtg
     1741 ctggtcggcg gatacggccg gcagaaccgc gccggtttgc tgcaggtgta ccgcgcgctg
     1801 cagccggcgg tggatgaccg actgccggag cggggcagtc tgccgccgct ggcgcgtttg
     1861 ctggtgactt cgccggtgcc ggatttaccc gagcggcggg acgaatgccg gcggagctgg
     1921 cgtgaggatt tcgtgctggc gccggaagcg acgcagttcg agattccgtt cgatggccgg
     1981 ctgctgttcg aggaacggct gctggcgatc gaggagccgg cttccccggc cgggctggcc
     2041 tatcggcaga tcgccgatgc actggccgga ttgcgggacg aaaaccagcg cacgcagaaa
     2101 caggcgagta tcgctgccga tatccatgac gacgatccgc gccggaccgg cgccgagcgg
     2161 gtggtgcgcg gcaagcgttt cgaggagcgg gtcgcccgcc tgctgacgct gctcgattac
     2221 cgggtcgagc ccgagcaata cctcgacaat gccaacaagg tcgatctgat tgcccgcagg
     2281 acggccggtc tgcggcagga atgctttctg gtcgaatgca aggattggga gaagccggtt
     2341 ccgaaagcgg aactggagcg attccgctcg tggctgaacg gtccgcaggc tcagcagatg
     2401 cgggccgagg gcttgtatgt cgcgcgctcc tatacgcgcg atgccctgag ttacgcgaaa
     2461 tcccaaggct tgatcgccgt cacgccgcag gagctggaag cgcaactgtt cgacttccgg
     2521 ccttatcttg atcgcttgcg ccggcgtttc gaggaaagcc cgctggcgcg gcattacgtg
     2581 gcgcagcggg tgttgctgga aagcgagccg gagctgaaag acggcgttga cctgatcgag
     2641 cacgcgcagg cctgggcaaa cggtgccggg cggcgcttgt ggctggtgct cggcgattac
     2701 ggcaccggca agtcatcc
//
LOCUS       DAOQJH010000413         2712 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_26320_length_2712_cov_4.393677, whole genome shotgun sequence.
ACCESSION   DAOQJH010000413 DAOQJH010000000
VERSION     DAOQJH010000413.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2712)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2712)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2712
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26320_length_2712_cov_4.393677"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            325..>2712
                     /gene="ligA"
                     /locus_tag="PLQ18_09610"
     CDS             325..>2712
                     /gene="ligA"
                     /locus_tag="PLQ18_09610"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008449329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HNE01952.1"
                     /translation="MSHLDPHARLLQLRDEIRRHEYLYYVLDQPEVPDAVFDALVIEL
                     RGIEDGHPQWITPDSPSQRVGGRPAAGFAPVRHALPMLSLDNAFTEADVRDFDRRARE
                     RLAAAGIEVDAIAYSAEPKLDGLAVSLRYEHGLLRQAATRGDGTTGEDITANVRTIRA
                     IPLRLAGGGWPEVLEVRGEVFMLRRDFERLNERARERGDKTFANPRNAAAGSLRQLDP
                     AVTAGRRLSFYAYGLGEVSDERALPGEHSAIIERLGDWELPVCPQRAVVFGPDGCLGY
                     YAGIGVQRAALAYDIDGVVYKVDCLDWQRRLGFVARAPRWASAHKYPAQEQLTTVEAI
                     DVQVGRTGAITPVARLAPVFVGGVTVTNATLHNFDEVARKDVRIGDTVIVRRAGDVIP
                     EVAGVLLDRRPMRSLLDPEHEPWQPPQHCPVCGSHIERPEGEAIARCSGGLTCAAQRK
                     EAIRHFAARRALDIDGLGERYIENLVDLGYVHGVADLYRLTLDDFLEMKRRADERDGI
                     TPETVAQGRIATKWAENLIGALARSRHTTLARLLFALGIRHVGESTAKTLADWLGALE
                     RVRHAPAPVLRALPDIGPEVAEAIDEFFLQPGNQQALDALLAGGVQPADEHPPAAALA
                     ERLNPVALLAALGAPKLSEARARQLAPALGAQGLAALPDLLADEARLAGTGLPAGHAQ
                     ALRDWLAVPESRARVQRTAGFAAGLLALLPAAPLIVRPLDGLTVVLTGTLSAMTRDEA
                     RARLEALGAKVAGSVSKKTGYVVAGAEAGSKLAKAQELGVEVLDEAAFLQRLAQWE"
BASE COUNT          373 a         1029 c          955 g          355 t
ORIGIN      
        1 ctgcactcct gcaggcggcg ggcgcctgaa cgacggcggt gaacggccgg gcggggcgga
       61 tcaatcgcgc gcccgctccc tgtcacccgg cctgctttcc agcccctctc cgcaccggtc
      121 gtcgccatcg cccgaccggc agaccctgcc gacccccgcc cggcgtcgag gcggagaggg
      181 ggacggcggg aaaaccttcc tgccgatcac gcatgaacgc cctggttcat cccctctctc
      241 caagcctcac ccgcgagggg ggataggctg aaaagaccgg gcgttcagct gcgagaccga
      301 catgacctgc tgcgcgtgac ccgcatgagc cacctcgatc cccacgcccg gctgctgcag
      361 ctgcgcgacg agattcgccg gcacgagtac ctgtactacg tgctcgacca gcccgaagtc
      421 cccgatgcgg tgttcgatgc gctggtcatc gaactgcgcg gcatcgagga cggacatccg
      481 cagtggatca cgccggattc gccgagccag cgcgtcggcg gcaggccggc ggcgggcttt
      541 gcgccggtgc gccacgcgct gccgatgctg tcgctcgaca acgccttcac cgaggccgac
      601 gtgcgcgact tcgaccgccg cgcgcgcgag cggctggccg cggccggcat cgaggtggat
      661 gcgatcgcct attcggccga gccgaagctc gacggcctcg ccgtcagcct gcgctacgag
      721 cacggcctgc tccgtcaggc cgcgacccgc ggcgacggca cgaccggcga ggacatcacc
      781 gccaacgtgc gcacgatccg cgcgatcccg ctgcgcctcg ccggcggcgg ctggccggag
      841 gtgctggagg tgcgcggcga ggtgttcatg ctgcgccgcg acttcgagcg cctgaacgag
      901 cgcgcccgcg aacgcggcga caagaccttc gcgaacccgc gcaacgccgc cgccggcagc
      961 ctgcgccagc tcgacccggc ggtgaccgcc gggcggcggc tgtcgttcta cgcctacggt
     1021 ctcggcgagg tctcggacga gcgcgcgctg cccggcgagc actcggcgat catcgagcgg
     1081 ctcggcgact gggagctgcc ggtctgcccg cagcgtgcag tggtcttcgg cccggacggc
     1141 tgtctcggtt attacgccgg catcggcgtg cagcgcgcag cgcttgccta cgacatcgac
     1201 ggtgtcgtct acaaggtcga ttgccttgac tggcagcgcc ggctcggctt cgtcgcgcgc
     1261 gcgccgcgct gggcgagcgc gcacaagtac ccggcgcagg agcagctcac cacggtcgag
     1321 gccatcgacg tgcaggtcgg gcgcaccggc gcgatcacgc cggtcgcgcg gctcgcgccg
     1381 gtgttcgtcg gcggcgtcac cgtcaccaat gccacgctgc acaacttcga cgaggtcgcg
     1441 cgcaaggacg tgcgcatcgg cgacaccgtc atcgtgcgcc gcgccggcga cgtgatcccc
     1501 gaggtcgccg gcgtgctgct cgatcgccgg ccgatgcgca gcctcctcga tcccgagcac
     1561 gagccctggc agccgccgca gcactgcccg gtgtgcggct cgcacatcga gcggcccgaa
     1621 ggcgaggcga tcgcgcgctg cagcggcggc ctgacctgtg ccgcgcagcg caaggaggcg
     1681 atccgccact tcgccgcgcg ccgcgcgctc gacatcgacg ggctcggcga gcgctacatc
     1741 gagaacctcg tcgatctcgg ctacgtgcac ggcgtcgccg acctgtaccg gctgacactc
     1801 gatgacttcc tggagatgaa gcgccgcgcc gacgagcgcg acggcatcac gcccgagacc
     1861 gttgcgcagg gcaggatcgc gacgaagtgg gccgagaacc tgatcggggc cctggcgcgc
     1921 agccggcaca cgacgctcgc gcgcctgctg ttcgcgctcg gcatccgcca cgtcggcgaa
     1981 tcgaccgcga agacgctcgc cgactggctc ggtgcgctcg aacgcgtgcg ccacgcgccg
     2041 gcaccggtgc tgcgcgcgct gcccgacatc ggtcccgagg tcgccgaggc catcgacgag
     2101 ttcttccttc agcccggcaa ccagcaggcg ctcgatgcgc tgctcgccgg cggcgtgcag
     2161 cccgccgacg aacatccgcc ggccgccgcg ctcgccgaac gtctgaaccc ggtcgcgctg
     2221 ctcgccgcgc tcggcgcccc gaagctcagc gaagcccgcg cccgccagct cgcgccggcg
     2281 ctcggggcgc aggggctggc cgcgctgccg gacctgctcg ccgatgaggc gcgcctcgcc
     2341 ggcaccggac tgccggccgg gcacgcacag gcgctgcgcg actggctcgc cgtgccggaa
     2401 tcccgcgcgc gcgtgcagcg caccgccggt ttcgcggccg gactgctcgc gctgctgccc
     2461 gccgcgccgc tcatcgtccg gccgctcgat ggcctcaccg tcgtgctgac cggcaccttg
     2521 tcggcgatga cccgcgacga ggccagggcc cgcctcgaag ccctcggcgc gaaggtcgct
     2581 ggcagcgtct cgaagaagac cggctatgtc gtcgccggcg ccgaggccgg cagcaagctc
     2641 gcgaaggcgc aggaactcgg cgtcgaggtg ctcgacgagg cggcgttcct gcagcggctg
     2701 gcgcagtggg ag
//
LOCUS       DAOQJH010000414         2710 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_26360_length_2710_cov_4.175518, whole genome shotgun sequence.
ACCESSION   DAOQJH010000414 DAOQJH010000000
VERSION     DAOQJH010000414.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2710)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2710)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2710
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26360_length_2710_cov_4.175518"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..243)
                     /locus_tag="PLQ18_09615"
     CDS             complement(<1..243)
                     /locus_tag="PLQ18_09615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE01953.1"
                     /translation="MASNPKEDAGRTPAAARAPRSRGLLGTVLLGLVLLVAVLALNPV
                     YTVESGEVVVLSTFGRYDPEPRLPGLHFRMPLVQSTH"
     gene            324..707
                     /locus_tag="PLQ18_09620"
     CDS             324..707
                     /locus_tag="PLQ18_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6394 family protein"
                     /protein_id="HNE01954.1"
                     /translation="MNLEKVVFGFFILLAATLNFGFVLGEIDNPVHHDVYELAAALVV
                     SLIATVLKLGDRTHMGAVLLATSLVADLQLIAAAVVWAIAAHAYGAGEAPRVVSSVVS
                     LASGAALANFISIVMLLAETINLRR"
     gene            720..2537
                     /locus_tag="PLQ18_09625"
     CDS             720..2537
                     /locus_tag="PLQ18_09625"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HNE01955.1"
                     /translation="MNLVHLLLQRRRPGSGGDAAPLDPREGSAAAINSVVFLFLRRMR
                     LPLVVLISVYAVSIAGLVAIPGVDPEGRPWRFDLFHAFYFISYTGSTIGFGEVPYAFT
                     PAQRLWVTVCIYLTVIGWLYAVGTILNLLQDPAFRRALAESRFAGEVRGLAEPFWIIC
                     GYGEAGSLLVRALCRRGVQTVVLDADAERLARLDLEDVGFDVPCLAADARASRILRLA
                     GLVHPHCLGVVAIAADDAIDVKVAITAKLLNPRLRVIARAEHASTAANLASFETDRII
                     DAYETFGEHLAMALVEPSLHMLYEYLIRQPGQPLPPRLVPPRGQWIVCGHGRFGSAAY
                     RHLAGAGLPVRVIESDPDDAPEDAVLGDATTANTLCSAGALSAAGLVAGTDDDADNLS
                     IAYTARLLNPDLFVIARANHREEEPLFAAAGLDLVISPSRLIVWRVLGELIAPQTNRF
                     LALAQRRGEAWAAPLLARLDACSEGLTPDTWETTIDAAQAPALHALLAAGRRPDFAAL
                     LRDPQDRSRPLPVLLLLRVGADGHEQLLPPPEAQPVAGDRLLFAGRSGSAATLAWIFG
                     SPKTLEYVLDGVEHPDGTVWRWLARRRAAHPRPVQGNTP"
BASE COUNT          338 a          980 c          967 g          425 t
ORIGIN      
        1 gtgcgtgctc tgcaccagcg gcatgcggaa gtgcaggccg ggcaggcgcg gttcggggtc
       61 gtagcggccg aaggtcgaca gcacgacgac ctcgccgctc tcgaccgtgt acaccgggtt
      121 cagcgccagc accgccacca gcagcaccag ccccagcagc acggtgccga gcaggccgcg
      181 cgagcggggc gcgcgggcag cggccggggt gcggccggcg tcctctttcg ggttcgaagc
      241 catgttccct cccgggcttt cacgaacggc ggcggccggc tgcctacact cggccgacaa
      301 cgacaccgcc agggggatgg tgcatgaatc tcgaaaaggt cgtcttcggc ttcttcatcc
      361 tgctcgccgc gacgctgaac ttcggcttcg tgctcggcga gatcgacaat cccgtgcatc
      421 acgacgtcta cgagctggcc gcggcgctgg tcgtgagcct gatcgcgacg gtgctgaagc
      481 tcggggatcg cacgcacatg ggcgcggtgc tgctcgcgac cagtctggtc gcggacctgc
      541 agctgatcgc ggcggccgtg gtctgggcga tcgcggcgca cgcctacggg gccggcgagg
      601 caccgcgggt cgtgtcctcg gtggtgtcgc tcgccagcgg cgcggcgctc gcgaacttca
      661 tctcgatcgt catgctgctc gccgaaacca tcaacctgcg ccgctgacga ccgccgaaga
      721 tgaacctcgt ccacctgctg ctgcagcgtc gccggcccgg ctccgggggc gacgcggcgc
      781 cgctcgatcc gcgcgagggc agcgcggcgg cgatcaacag cgtcgtgttc ctgttcctgc
      841 ggcgcatgcg cctgccgctg gtggtgctga tcagcgtcta cgcggtgtcg atcgccgggc
      901 tggtcgcgat tccgggtgtc gatccggagg gccggccgtg gcgcttcgac ctgttccacg
      961 ccttctactt catcagctac accggttcga cgatcggctt cggcgaggtg ccgtacgcgt
     1021 tcacgccggc gcagcggctg tgggtgacgg tgtgcatcta cctgaccgtg atcggctggc
     1081 tgtatgcggt cggcacgatc ctgaacctgc tgcaggaccc ggccttccgc cgggcgctgg
     1141 ccgagagccg cttcgccggc gaggtgcgcg ggctggccga gccgttctgg atcatctgcg
     1201 gctacggcga ggccggcagc ctgctggtgc gggcgctgtg ccggcgcggc gtgcagacgg
     1261 tcgtgctcga tgccgatgcc gagcgcctcg cgcggctcga cctggaggac gtgggcttcg
     1321 acgtgccgtg cctcgcggcc gatgcgcgcg cctcgcgcat cctgcggctg gccgggctcg
     1381 tgcatccgca ctgcctcggc gtggtcgcga tcgctgccga cgatgcgatc gacgtgaagg
     1441 tcgcgatcac cgcgaagctg ctgaacccgc gcctgcgcgt gatcgcgcgc gccgagcacg
     1501 cctcgaccgc ggccaatctc gcgtcgttcg agaccgaccg catcatcgat gcctacgaga
     1561 ccttcggcga gcatctggcc atggcgctgg tcgagccgag cctgcacatg ctctacgaat
     1621 acctgatccg ccagcccggc cagccgctgc cgccgcggct ggtgccgccg cgcgggcagt
     1681 ggatcgtctg cggtcacggg cgcttcggca gcgccgcgta ccggcatctg gccggcgccg
     1741 gcctgccggt gcgcgtgatc gagtccgatc cggacgatgc gcccgaggat gccgtgctcg
     1801 gtgatgcgac gaccgccaac acgctgtgct cggccggggc gctcagtgcg gccgggctgg
     1861 tcgccggcac cgacgacgac gccgacaacc tgtcgatcgc ctacacggcg cggctgctga
     1921 atccggacct gttcgtgatc gcacgcgcca atcaccgcga ggaggagccg ctgttcgcgg
     1981 ccgccggcct cgatctggtg atctcgccga gccggctgat cgtctggcgc gtgctcggcg
     2041 agctgatcgc gccgcagacc aaccgcttcc tcgcgctcgc gcagcgccgc ggcgaggcct
     2101 gggccgcgcc gctgctggca cggctcgacg cctgcagcga gggcctgacg cccgatacct
     2161 gggagacgac gatcgacgcc gcgcaggccc cggccctgca cgcgctgctc gccgccgggc
     2221 ggcggccgga cttcgcggcg ctgctgcgcg acccgcagga ccgcagccgg ccgctgccgg
     2281 tgctgctact gctgcgcgtc ggcgccgatg gccacgagca gctgctgccg ccgcccgagg
     2341 cgcagccggt cgccggcgac cggctgctgt tcgccggccg gagcggcagc gcggcgacgc
     2401 tcgcgtggat cttcggcagc ccgaagacgc tcgaatacgt gctcgatggc gtcgagcacc
     2461 ccgacggcac ggtgtggcgc tggctcgcac gccggcgggc cgcgcatccg cggccggtgc
     2521 agggaaacac tccttaacgt gcccctaagt cggcggcgac taggcttgcc tccatcgagc
     2581 cggacggcaa gccggagcga tatctcccag gtcacgcagc atgctggggg cgaccggcag
     2641 acccggtcgc cccctctttt tttgcctgcg ccttccgcct gccctgcctg cctcgtgccc
     2701 gccggttttc
//
LOCUS       DAOQJH010000415         2704 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_26471_length_2704_cov_4.040393, whole genome shotgun sequence.
ACCESSION   DAOQJH010000415 DAOQJH010000000
VERSION     DAOQJH010000415.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2704
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26471_length_2704_cov_4.040393"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(114..2144)
                     /locus_tag="PLQ18_09630"
     CDS             complement(114..2144)
                     /locus_tag="PLQ18_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019644557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl/propionyl/methylcrotonyl-CoA carboxylase
                     subunit alpha"
                     /protein_id="HNE01956.1"
                     /translation="MFDKILIANRGEIACRVIASCRRLGIRSVAVYSDADAQALHVGL
                     ADEAVRIGPAKAADSYLCAARIVGAALASGAQAVHPGYGFLSENAAFAEAVEAAGLVF
                     IGPPPAAIRAMGSKSAAKVRMAQAGVPVVPGYHGEDQSLERLTAEAIATGFPLLLKPS
                     AGGGGKGMKVVTHAGELGAQIESARREAAASFGDEQLLIERYLERPRHIEIQIFADAH
                     GRCVSLFERDCSLQRRHQKVIEEAPAPGLDPARRQAMGEAACTAALAIGYRGAGTVEF
                     IAEHEEAGDGRFFFMEMNTRLQVEHPVTEMITGLDLVEWQLRVAAGEPLPLAQAQIPL
                     HGHAIEARIYAEDPARDFLPASGRLVHLHWPAGDAHVRIDTGVRQGDAVTPHYDPLLA
                     KLIVHDEDRPRALARLRRALADCRIVGVANNLRFLAALAAHPGYAAGAVHTGFIADQR
                     ATLLPAPATAPMRVLALATLGRLLKAARDAARRAAASAEPDSPWQHGDGWRLNEDNHQ
                     VLRYRDGDDGPVQAVIAHYRPGHWRLELPDGSTATVAGRLDDEDVVHAEIDGRRTRAT
                     LVRTGAQLHVLGLDGAWTLTLHDPLAQAMAGEAAGAGRLSAPMPGTVVAVRVAPGEAV
                     EAGQVLLILEAMKMEHTLRAPRAGTVASIACTVGETVTEGTELLKLAGEEEG"
     gene            2217..2570
                     /locus_tag="PLQ18_09635"
     CDS             2217..2570
                     /locus_tag="PLQ18_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011519197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HNE01957.1"
                     /translation="MSIRIEDLNKLDFTDVIDPGAAPVPPTHPGEVLLHDFMEPLGLS
                     ANALARALHVPANRITAIVNGTRGISGDTALRLARYFGGDAQWWMHLQSRHELLSARL
                     ASGADIERSVQPRVA"
     gene            complement(2589..>2704)
                     /locus_tag="PLQ18_09640"
     CDS             complement(2589..>2704)
                     /locus_tag="PLQ18_09640"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011492297.1"
                     /note="Catalyzes the reversible hydration of unsaturated
                     fatty acyl-CoA to beta-hydroxyacyl-CoA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HNE01958.1"
                     /translation="ALIDDTARRIARLRASPEGREGVSAFLDKRAPAWRDG"
BASE COUNT          371 a         1010 c          933 g          390 t
ORIGIN      
        1 tttttctacc cctctcccca acccctctcc cgcaaggggc gaggggctga aaagcgaagt
       61 gcgccggtgg gatcggtatg cgtgcacccc ggcaggcacg ggcacaccgc cgttcagccc
      121 tcttcctcgc cggccagctt cagcagctcc gtgccttccg tgaccgtctc gcccacggtg
      181 caggcgatgc tggcgaccgt gccggcgcgc ggggcgcgca gcgtgtgttc catcttcatc
      241 gcttcgagga tcagcaatac ctgtccggcc tcgaccgcct cgcccggtgc gacccgcacc
      301 gcgaccaccg tgcccggcat cggggcgctc aggcgcccgg ccccggccgc ctcgccggcc
      361 atggcctgcg ccagcggatc gtgcagcgtc agcgtccagg cgccgtcgag gccgagcacg
      421 tgcagctgtg ccccggtgcg caccagcgtc gcgcgcgtgc gccggccgtc gatttcggcg
      481 tgcacgacat cctcgtcatc gagccggccg gcgaccgtcg ccgtcgagcc atccggcagt
      541 tccagccgcc agtgcccggg ccggtagtgc gcgatcacgg cctggaccgg gccatcgtcg
      601 ccatcgcgat aacgcagcac ctgatggttg tcctcgttga gccgccagcc gtcgccgtgc
      661 tgccacggcg aatccggctc ggcgctggcg gcggcgcggc gcgcggcatc gcgcgcggcc
      721 ttcagcagcc gcccgagcgt cgccagcgcc agcacgcgca tcggcgccgt ggccggcgcc
      781 ggcagcaggg tcgcgcgctg gtcggcgatg aagccggtat gcacggcgcc ggccgcgtat
      841 ccggggtggg ccgcgagcgc ggcgaggaag cgcaggttgt tcgccacccc gacgatgcgg
      901 cagtcggcga gcgcccgccg cagccgcgcc agcgcgcgcg ggcggtcctc gtcgtggacg
      961 atcagcttcg cgagcagcgg gtcgtagtgc ggcgtgaccg catcgccctg gcgcacaccg
     1021 gtgtcgatgc gcacgtgcgc atcgcccgcc ggccagtgca ggtgcacgag ccggccgctc
     1081 gccggcagga agtcgcgcgc cggatcctcg gcgtagatgc gcgcctcgat cgcgtggccg
     1141 tgcagcggga tctgcgcctg cgcgagtggc agcggttcgc cggcggcgac acgcagctgc
     1201 cattcgacca gatcgaggcc ggtgatcatt tccgtgaccg gatgctcgac ctgcaggcgc
     1261 gtgttcatct ccatgaagaa gaaccgcccg tcgccggctt cctcgtgctc ggcgatgaac
     1321 tcgacggtgc cggcgccgcg atagccgatc gccagcgccg ccgtgcaggc cgcctccccc
     1381 atcgcctgcc ggcgcgccgg atcgagaccc ggggccgggg cttcctcgat gaccttctgg
     1441 tgccggcgct gcagcgagca gtcgcgctcg aacagcgaca cgcagcggcc gtgggcatcg
     1501 gcgaagatct ggatctcgat gtgacgcggg cgttcgaggt agcgctcgat cagcagctgc
     1561 tcatcgccaa agctcgctgc cgcctcgcgc cgcgccgact cgatctgcgc accgagctcg
     1621 cccgcgtgcg tgacgacctt catgcccttg ccgccgccgc cggcgctcgg cttcagcagc
     1681 agcggaaagc cggtggcgat ggcttcggcc gtgagccgtt cgagcgactg atcctcgccg
     1741 tgatagcccg gcaccaccgg cacgccggcc tgcgccatgc gcaccttggc cgcgctcttc
     1801 gagcccatcg cccggatcgc cgccggcggc ggcccgatga acacgagccc ggcggcctcg
     1861 accgcctcgg cgaacgccgc gttctcggac aggaagccgt agcccggatg cacggcctgc
     1921 gcaccgctcg cgagcgcggc cccgacgatg cgcgcggcac acagatagct gtcggccgcc
     1981 ttcgccggcc cgatgcgcac ggcctcgtcg gcaagcccga cgtgcagcgc ctgcgcatcg
     2041 gcatccgaat acacggcgac gctgcggatg ccgagccgcc ggcagctcgc gatcacccgg
     2101 caggcgattt cgccgcggtt ggcgatgagg atcttgtcga acatgatcat gtcccgtacc
     2161 ggttcggtgc ggaccacagc atggacgcga tgacgctgtc cggattcggg ggttgcatga
     2221 gcattcgcat cgaggacctg aacaagctcg atttcacgga tgtgatcgat ccgggcgcag
     2281 caccggtgcc gccgacgcat ccgggcgagg tgctgctgca tgacttcatg gagccgctcg
     2341 gcctgtccgc gaacgcgctg gcccgggcgc tgcacgtgcc ggccaatcgc atcaccgcca
     2401 tcgtcaacgg cactcgcggc atcagcggcg ataccgccct gcggctggca cgctacttcg
     2461 gtggcgacgc gcaatggtgg atgcacctgc agtcgcgtca cgagctgctg agcgcccggc
     2521 tcgcgagcgg cgcggacatc gaacgctcgg tgcaaccgcg cgtcgcctaa aaacgcttca
     2581 ccccggcatc agccgtcacg ccacgccggc gcccgcttgt cgagaaacgc cgaaacccct
     2641 tcgcggccct cggggcttgc acgcaggcgg gcgatgcgcc gggcggtgtc gtcgatcagc
     2701 gccg
//
LOCUS       DAOQJH010000416         2701 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_26531_length_2701_cov_3.236584, whole genome shotgun sequence.
ACCESSION   DAOQJH010000416 DAOQJH010000000
VERSION     DAOQJH010000416.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2701
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26531_length_2701_cov_3.236584"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2451
                     /locus_tag="PLQ18_09645"
     CDS             <1..2451
                     /locus_tag="PLQ18_09645"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNE01959.1"
                     /translation="RLRVRLETLLAAGAWPAEVWLVAADGQVLARLRDEARLRDVLPP
                     EVVQLASGTLRAPSDRLFAWSPVGTGGWRLVLRQPLDAISAPSTLLLPGIWLLAGLAG
                     SFLLLFWQLRRRARRISGQMVRPLRAIEGLVRRIGRGDYTPPPPKLALLELQDTADAV
                     LEMGRRLGEANCLLVEQGRDLRRQAGFRQLLIDSVPVPLFYTDAGGRLLGGNLAFRAF
                     VGTRSDGGEAMARRLLQALPEAAPGLPAAAETLFDDGLDGPRHLLVSGAGFSGVGEAD
                     GGQVGVILDLTERRRSERELEAARDRALAASRQKSDFLASVSHEIRTPMNGVIGMTEL
                     LLHTGLSAEQHDYARTILGSAQALLQLLNDILDFSKIEAGQITLAADPFDPATLVEGA
                     AELLAARAWEKRIVLATRVDPRLPVPLYGDAGRVRQVLLNLIGNAVKFTDAGEVIVAA
                     EALSHDGRQALVRFSVTDTGLGIPDSARPRMFRPFSQLEGQGERRYAGTGLGLSICKR
                     LCELMGGDISFDSEPGRGSVFRVTLPFRRTGELALLEAGAGPAPAGSTALPVLPPAGV
                     LLDGLPAGAHAALAGTLRAFGLSVYDDQGIEAMAPALRLVLCGAAPEALRAQAAYPSL
                     PAVLLATRDEAGLAARAEAQGWSAVLALPLRRAALHEILGALLYGRSGSGTAPNGTLP
                     ARAAVVPRGATLLLAEDDRTNQRVASAHLRKLGFAVEVVGDGEAAVKAATGRHYPLIL
                     MDVQMPVMDGLQAAAAIRRAQAGGPASIIVALTANAMSGDRERFLAAGMDDYLSKPFT
                     GDALRRLLERWLPPEGEG"
     gene            complement(2461..>2701)
                     /locus_tag="PLQ18_09650"
     CDS             complement(2461..>2701)
                     /locus_tag="PLQ18_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017735316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HNE01960.1"
                     /translation="GPVLASLLGLGLICSALAYGLYYRLIADLGPTRALTVTFLIPPF
                     GVAWSALLLGEPLSPALAGSGVLIVAGTVLVLRGR"
BASE COUNT          338 a         1001 c          999 g          363 t
ORIGIN      
        1 cgtctgcgcg tgcggctgga aaccctgctc gccgccggtg cctggccggc cgaggtctgg
       61 ctggtggccg ccgacggtca ggtgctcgcg cgcctgcgcg acgaggcacg gctgcgcgac
      121 gtgttgccgc ccgaggtcgt gcagcttgcc tccggcacgc tgcgcgcacc gagtgatcgt
      181 ctgttcgcgt ggtcaccggt cggcaccggc ggctggcggc tggtgctccg ccagccgctc
      241 gatgcgatca gcgcgccctc gacgctgctg ctgcccggca tctggctgct cgccgggctc
      301 gccggcagct tcctgctgct gttctggcaa ctgcgccggc gggcgcggcg gatttccggg
      361 cagatggtgc ggccgctgcg cgcgatcgaa gggctggtgc ggcgcatcgg ccgcggcgac
      421 tacacgccgc cgccaccgaa gctcgcgctg ctcgaactgc aggacaccgc tgatgccgtg
      481 ctggagatgg gccggcgcct cggtgaggcc aactgcctgc tggtcgagca ggggcgcgac
      541 ctgcgccggc aggccggctt ccggcagctg ctgatcgaca gcgtgccggt gccgctgttc
      601 tacaccgacg ccggcggccg gctgctcggc ggcaatctgg ccttccgcgc cttcgtcggc
      661 acgcgcagcg acggcggcga ggcgatggcg cgccggctgc tgcaggcgct gcccgaagcc
      721 gcgcccggtc tgccggcggc cgccgaaacc ctgttcgacg acggtctcga cggcccgcgc
      781 cacctgctgg tgtccggcgc cggcttcagc ggggtcggcg aggcggacgg cggtcaggtc
      841 ggcgtcatcc tcgacctgac cgagcgccgg cgcagcgagc gcgagctcga agccgcccgc
      901 gaccgcgcgc tcgccgcctc gcgacagaaa tccgactttc tggcctcggt cagccacgag
      961 atccgcacgc cgatgaacgg cgtcatcggc atgaccgagc tgctgctgca caccgggctg
     1021 agcgccgagc agcacgatta cgcccgcacg atcctcggct cggcgcaggc gctgctgcag
     1081 ctgctgaacg acatcctcga cttctcgaag atcgaggccg gccagatcac gcttgccgcc
     1141 gatcccttcg atccggcgac gctggtcgaa ggcgcggccg aactgctggc ggcgcgggcc
     1201 tgggaaaaac gcatcgtgct cgcgacgcgc gtcgatccgc gcctgccggt gccgctgtac
     1261 ggcgacgccg ggcgcgtgcg ccaggtgctg ctgaacctga tcggcaacgc cgtgaagttc
     1321 accgatgccg gcgaggtcat cgtcgccgcc gaggcgctga gccacgacgg ccgccaggcg
     1381 ctggtgcgct tctcggtcac ggataccggc ctcggcatcc ccgactcggc gaggccgcgc
     1441 atgttccggc cgttcagcca gctcgaaggc cagggcgagc gccgctatgc cggcaccggg
     1501 ctggggctgt cgatctgcaa gcggctctgc gagctgatgg gcggcgacat cagcttcgac
     1561 tccgagccgg ggcgcggctc ggtgttccgg gtgacgctgc cgtttcggcg caccggtgag
     1621 ctggcgctgc tcgaagccgg tgccggcccg gcgccggccg ggtccaccgc gctgccggtg
     1681 ctgccgccgg ccggcgtgct gctcgacggc ctgccggccg gcgcacatgc cgcgctcgcc
     1741 ggcaccttgc gtgcgttcgg cctgagcgtg tacgacgacc agggcatcga ggcgatggct
     1801 ccggcgctgc ggctggtgct ctgcggggcc gcgcccgagg cgctgcgcgc acaggccgcg
     1861 tatccgtcgc tgcccgccgt gctgctcgcg acccgcgacg aggccggact cgcggcgcgc
     1921 gccgaagccc agggctggtc ggcggtgctg gcgctgccac tgcgccgcgc ggcgctgcac
     1981 gaaatcctgg gcgcgctgct gtacggccgc agcggcagcg ggacggcccc caacggcacg
     2041 ctgccggcac gtgccgcggt cgtgccgcgc ggcgcgacgc tcttgctcgc cgaggacgac
     2101 cgcaccaacc agcgcgtcgc cagcgcacac ctgcgcaagc tcggcttcgc ggtcgaggtg
     2161 gtcggcgacg gcgaggccgc ggtcaaggcg gcgaccggcc gccattaccc gctgatcctg
     2221 atggacgtgc agatgccggt catggacggc ctgcaggcgg ccgcggcaat ccgccgcgcg
     2281 caggccggcg gcccggcctc gatcatcgtc gcgctgaccg ccaacgccat gtccggcgac
     2341 cgcgaacgct tcctcgccgc cggcatggac gactacctga gcaagccctt caccggcgac
     2401 gcgctgcgcc ggctgctgga gcgctggctg ccgccggaag gcgagggctg agcgcgaacc
     2461 tcagcgtccg cgcagcacca acactgtccc cgccacgatc agcacgccgc tgccggccag
     2521 cgccggcgac agcggttcgc cgagcagcaa cgcactccac gcgacgccga agggcgggat
     2581 caggaaggtc acggtcagcg cgcgcgtcgg gccgaggtcg gcgatcagcc ggtaatacag
     2641 cccgtaggcg agggcgctgc agatcaggcc gaggccgagc aggctcgcga gcaccgggcc
     2701 g
//
LOCUS       DAOQJH010000417         2701 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_26532_length_2701_cov_3.198791, whole genome shotgun sequence.
ACCESSION   DAOQJH010000417 DAOQJH010000000
VERSION     DAOQJH010000417.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2701
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26532_length_2701_cov_3.198791"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(245..772)
                     /locus_tag="PLQ18_09655"
     CDS             complement(245..772)
                     /locus_tag="PLQ18_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015954918.1"
                     /note="PEP-CTERM proteins occur, often in large numbers,
                     in the proteomes of bacteria that also encode an
                     exosortase, a predicted intramembrane cysteine proteinase.
                     The presence of a PEP-CTERM domain at a protein's
                     C-terminus predicts cleavage within the sorting domain,
                     followed by covalent anchoring to some some component of
                     the (usually Gram-negative) cell surface. Many PEP-CTERM
                     proteins exhibit an unusual sequence composition that
                     includes large numbers of potential glycosylation sites.
                     Expression of one such protein has been shown restore the
                     ability of a bacterium to form floc, a type of biofilm;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HNE01961.1"
                     /translation="MLALAPFTQASALLWNWSYSGTGITAGGTFTTDDTPDGSGHYLI
                     SAITGSRNGIAITGLMPAGAPIPGNEPFNIDNLVSSGSEQLTGDGFGYQLADGNYVSP
                     FFASFLSPPGYLEVYSAAPFTPGFTNFGPEDSELPISFSAVPVPEPVGLALLAAGGGV
                     LGVLRRRTRAARPQA"
     gene            complement(933..1433)
                     /locus_tag="PLQ18_09660"
     CDS             complement(933..1433)
                     /locus_tag="PLQ18_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HNE01962.1"
                     /translation="MAGLSGPLGQLLATLPQTGRVDWIGLRPAPRAPMQEVDAVLAEA
                     GRGLAGDRWAGRPDGARQLTLLQAEHLPVIAGCAGLAAIEPARLRRNLVISGLNLLAL
                     KGRRFRLGEALCEYSGLCQPCSRMEAALGPGGWNAMRGHGGITARVLESGWVRIGDAL
                     AVEHLP"
     gene            complement(1421..2119)
                     /locus_tag="PLQ18_09665"
     CDS             complement(1421..2119)
                     /locus_tag="PLQ18_09665"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNE01963.1"
                     /translation="MTVAIRALSFDLDDTLWNIWPCIERAEAKLHDWLLAGYPRIAAQ
                     FDALALRRLCSDEATRDPAIAHDRSLIRRRGLTRAAELAGYAEFALDEAFEVFFAARN
                     QVEFFADALPVLERLAARYPVVALSNGNADLDRIGIAHLFAFHLSAAIVGTQKPHPAM
                     FEQAVARLGLPPAAILHVGDDPEHDVEGARAAGLRTVWVRRDGRDWPHGLAPPEFVIH
                     DLHELEALLATWPG"
     gene            2302..>2701
                     /locus_tag="PLQ18_09670"
     CDS             2302..>2701
                     /locus_tag="PLQ18_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020406387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HNE01964.1"
                     /translation="MLRFRGSPALSAFRRDKLLAAVQARVPAVRGVYAEYVHFVDGRL
                     DEAGTAVMTQLLEYGPALEARTGTTPPLLLVVPRPGTISPWSSKATDIAHACGLDEVR
                     RVERGIAWWFDGAGAADRPLLAPLVHDRMVE"
BASE COUNT          370 a          939 c         1013 g          379 t
ORIGIN      
        1 gacccatgcg tgagccttgc agaccctggc gcggcacacg cgattccttc gtgcggcagg
       61 caccgaccca tgcgcaaggc ttgcacttgc ctgccggagg cttgcggacc tgtcggaaag
      121 acttgcggac cttgccgcgc ggcttgcgct tgcctgcgcg cgacgtgcgg atgcctgcac
      181 ggccagcgtg gccggggtcg gcgaccccgg tcgtggtcgg cggggatggc tgcgcgcacc
      241 ggcctcaggc ttgcgggcgc gctgcgcgtg tgcgccgccg caacacaccg agcacgccgc
      301 cgcccgccgc aagcagcgcc agccccaccg gttccggcac cggcaccgcc gagaagctga
      361 tcggcagttc gctgtcctcg gggccgaagt tggtgaagcc gggcgtgaac ggggccgccg
      421 agtacacctc cagatagccg ggcggtgaca ggaagctggc gaagaacggg ctgacgtaat
      481 tgccgtcggc gagctggtag ccgaagccgt cgccagtcaa ttgctcactg ccggacgata
      541 cgaggttgtc gatgttgaac ggctcgttgc cgggaatcgg tgcgccggcc ggcatcaggc
      601 cggtgatggc gatgccgttg cggctgccgg tgatggcgct gatcaggtaa tggccgctgc
      661 cgtccggggt gtcgtcggtc gtgaacgtgc cgccggcggt gatgccggtg ccggaatagc
      721 tccagttcca cagcagcgcc gaagcctgcg tgaacggggc gagggcgagc attgcgccga
      781 gcagcacggc ggcgagacgg cgggcggggt gtcgggcgga gcgggtcgcg gacatgcagg
      841 actccgtggc gcaagggcca ggtctgggcg gagccccgag catggccggg cgcggcgtga
      901 gtcacggcaa gcagcgctgt ctccggagca aatcacggca ggtgttcgac cgcgagcgca
      961 tcgccgatgc gaacccagcc actttcgagc acgcgcgcgg tgatgccgcc gtggccgcgc
     1021 atggcgttcc agccgcccgg tccgagggcg gcttccatgc gcgagcaggg ctggcacagg
     1081 ccgctgtact cgcagagcgc ctcgccgagg cggaagcgcc ggcccttcag cgccagcagg
     1141 ttcaggccgg aaatcacgag attgcgccgc agccgtgccg gctcgatcgc ggccagtccg
     1201 gcacagccgg cgatcaccgg caggtgctcg gcctgcagca gcgtgagctg gcgtgcgccg
     1261 tcgggccggc cggcccagcg gtcgccggcc aggccgcggc cggcctcggc gagcaccgca
     1321 tcgacctcct gcatcggcgc acgcggcgcc gggcgcaggc cgatccagtc gacgcgcccg
     1381 gtctgcggca gcgtcgccag cagttgcccg agcggcccgc tcagcccggc catgtcgcga
     1441 gcagggcttc gagttcgtgc aggtcgtgaa tcacgaactc cggcggcgcg agtccgtgcg
     1501 gccagtcgcg cccgtcgcgc cgcacccaga ccgtgcgcag gccggccgca cgcgcgcctt
     1561 cgacatcgtg ctccggatcg tcgccgacgt gcaggatcgc cgccggcggc aggccgaggc
     1621 gcgcgacggc ctgctcgaac atcgccggat gcggcttctg cgtgccgacg atcgccgccg
     1681 acagatggaa cgcgaacagg tgtgcgatgc cgatgcgatc gagatcggcg ttgccgttgc
     1741 tgagcgcgac gaccgggtag cgcgcggcca ggcgttcgag caccggcagc gcatcggcga
     1801 agaactcgac ctggttgcgc gccgcgaaga acacctcgaa cgcctcgtcg agcgcgaatt
     1861 cggcgtaacc ggcgagttcg gcggcgcgcg tcaggccgcg gcggcggatc aggctgcggt
     1921 cgtgggcgat ggccgggtcg cgggtggcct cgtcgctgca gagccggcgc agcgccagcg
     1981 catcgaactg cgcggcgatg cgcggatagc cggcgagcag ccagtcgtgc agcttcgcct
     2041 cggcgcgctc gatgcacggc cagatgttcc acagcgtgtc gtcgaggtcg aagctcaggg
     2101 cgcggatggc gacggtcatg gcgcaggtct cggtcggacg cgatcgacgg gttgggaccc
     2161 cgggcgcgtg actttcgcat gaatccgcgc acggcagcgt gcgcgtgccc gccgggcgga
     2221 ccgccgtgct gcggcgcatc cggtacaatt ccgcgccttt tccgccccgc cccccgccgt
     2281 ttgccgagag gccgatcgcc catgctgcgc ttccgtggtt cccccgccct ttccgccttc
     2341 cgccgcgaca agctgctcgc ggccgtgcag gcgcgcgtgc ccgcggtgcg cggcgtctac
     2401 gccgaatacg tgcatttcgt cgacggccgc ctcgatgagg ccggcacggc ggtgatgacg
     2461 cagctgctcg aatacggtcc ggcgctcgaa gcccgcaccg ggacgacgcc gccgctgctc
     2521 ttggtcgtgc cgcggccggg cacgatctcg ccgtggtcgt cgaaggccac cgacatcgcg
     2581 cacgcctgcg ggctcgatga agtgcgccgc gtcgagcgcg gcatcgcctg gtggttcgac
     2641 ggcgccggcg cggccgatcg tccgctgctg gcgccgctcg tgcacgatcg catggtcgag
     2701 g
//
LOCUS       DAOQJH010000418         2700 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_26547_length_2700_cov_4.669943, whole genome shotgun sequence.
ACCESSION   DAOQJH010000418 DAOQJH010000000
VERSION     DAOQJH010000418.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2700
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26547_length_2700_cov_4.669943"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..396)
                     /locus_tag="PLQ18_09675"
     CDS             complement(<1..396)
                     /locus_tag="PLQ18_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012627858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HNE01965.1"
                     /translation="MTVPSYKRPQILALAPDPASAKAGEGLARRAKWLHYATDGRALW
                     GECQGSGSTPYRTQVDLAGPAFRCSCPSRKFPCKHGLGLMLLHADVPAASAETPDWVG
                     EWLAKRESRPATAPAAPATPPPPPADIVLL"
     gene            complement(536..1273)
                     /locus_tag="PLQ18_09680"
     CDS             complement(536..1273)
                     /locus_tag="PLQ18_09680"
                     /inference="COORDINATES: protein motif:HMM:NF015945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HNE01966.1"
                     /translation="MGIFSKIITAIRGGATEVGEAIVDSQAMRILDQEIRDAGEDLRL
                     AKDELTKLMAKRKLADEDVRALGSKVGEYEGYLGQVLAREAPDGSLAAQDAQLRDELA
                     AKIAELEGELGQKQSLAQEFAQRENSVKTAIRSAEGNLDKLKQRASMVKATDAVHKAQ
                     AQIASSHSGANARMTTAMDSLARLEQRQKEQSARFDAATQLEHETQSSERSLEDKLKA
                     SGLIGSGPSSSDVLARLRAKRLPDHSS"
     gene            complement(1310..1942)
                     /locus_tag="PLQ18_09685"
     CDS             complement(1310..1942)
                     /locus_tag="PLQ18_09685"
                     /inference="COORDINATES: protein motif:HMM:NF021457.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfI family protein"
                     /protein_id="HNE01967.1"
                     /translation="MEEQKKTSADYQRAFRERMRAAGYVKRDVWVPPEQSEALRACEL
                     ALRKGIVPIIVKRRRAGMGNTTNSFVWTTETLLAALTDTDAVRAGRLKVESIEGLEPC
                     IGIRFPELEDLPVFMIAGGTQLLVSTLLWRVDEVADPADFNKLLLRTHKILPLSTFGI
                     VTMPDGEYYELFGALSAASDLEEILEEIETLGDNVVQVYESFGSYLKRAD"
     gene            2076..>2700
                     /locus_tag="PLQ18_09690"
     CDS             2076..>2700
                     /locus_tag="PLQ18_09690"
                     /inference="COORDINATES: protein motif:HMM:NF022144.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2491 family protein"
                     /protein_id="HNE01968.1"
                     /translation="MRARTARENPGVNVVMGLLDSLFKLGKSRVREAVSGRKPERTLP
                     SALGLRIGASVEFDLLPVRMNAGAFRFTLPRTDEPMIVTAQGRFELGEGMIVHRFYSD
                     SSTMLQLLCRDLPGLEDQVEEITLYVPLESAYPENREQIHRWVGPQGRIGAPEFRIAD
                     GTRYRRIWFDDEPGPASPVRYTEEVFDEPDSFSRPRRIAQEAMLYGRE"
BASE COUNT          392 a          922 c          928 g          458 t
ORIGIN      
        1 gaggagcacg atgtcggcag gcggcggggg aggagttgcc ggagctgccg gggctgtcgc
       61 cgggcggctt tcgcgcttcg cgagccattc gccgacccag tccggggtct cggcactcgc
      121 ggccggcacg tcggcgtgca gcagcatcag gccgaggccg tgcttgcacg ggaacttgcg
      181 gctcgggcac gagcagcgga aggccggccc ggcgagatcg acctgggtgc ggtacggcgt
      241 gctgccgctg ccctggcatt cgccccacag cgcgcggccg tcggtggcgt agtgcagcca
      301 tttcgcacgt cgcgccagcc cttcgccggc tttcgcggaa gccgggtcgg gggcgagggc
      361 gaggatctgc gggcgcttgt acgacgggac cgtcaagagg ctaccggcat ggcgggaagg
      421 atggccggca gcatggacga tgaacggccg gtctgcagcc cgggcgcctc acgaaatccg
      481 ggcggccacc cgaggcggca cgccggcccg gcgcacgtgc cgccgcccgg cgggctcagg
      541 acgaatgatc cggcagacgt ttggcgcgca ggcgcgcgag cacgtcggag ctgctcggac
      601 cgctgccgat caggcccgaa gccttgagct tgtcctccag cgaacgctcg ctgctctgcg
      661 tctcgtgttc gagctgcgtg gcggcgtcga agcgcgccga ctgctccttc tggcgctgtt
      721 cgaggcgggc cagcgaatcc atcgccgtgg tcatgcgcgc gttggctccg gagtgcgagg
      781 acgcgatctg cgcctgcgcc ttgtgcacgg cgtcggtcgc cttgaccatc gaggcgcgct
      841 gcttgagctt gtcgaggttg ccctcggcgc tgcggatcgc ggtcttgacg ctgttctcgc
      901 gctgcgcgaa ctcctgcgcc agcgactgct tctggccgag ctcgccttcg agttcggcga
      961 tcttggccgc gagttcgtcg cgaagctgcg catcctgcgc tgccagcgag ccgtccggcg
     1021 cctcgcgcgc cagcacctgg ccgagatagc cctcgtattc gccgaccttg ctgccgagcg
     1081 cgcgcacgtc ctcatcggcg agcttgcgct tggccatcag cttggtcagt tcgtccttgg
     1141 ccaggcgcag atcctcgccg gcgtcgcgga tttcctgatc gaggatgcgc atcgcctgac
     1201 tgtcgacgat cgcctcgccg acctcggtgg caccgccgcg gatcgcggtg atgatcttgc
     1261 tgaagatgcc catggatgtc cggacctccg gaataagtct gtcagtcggt cagtcggcgc
     1321 gcttcaggta ggagccgaag gattcgtaga cctgcacgac gttgtcgccg agggtttcga
     1381 tttcttcgag gatttcctcg agatccgatg ccgcgctcag cgcgccgaac agctcgtagt
     1441 actcgccgtc aggcatcgtg acgatgccga aggtcgacag cggcaaaatc ttgtgcgtgc
     1501 gcagcagcag cttgttgaaa tcggccggat cggcgacctc gtcgacgcgc cagagcagcg
     1561 tgctgaccag cagttgcgtg ccgccggcga tcatgaacac cggcaggtct tccagttccg
     1621 ggaagcggat gccgatgcac ggctccagcc cctcgatgct ctcgaccttc agccggccgg
     1681 cgcgcaccgc gtcggtgtcg gtcagggccg ccagcagcgt ctccgtggtc cagacgaatg
     1741 aattcgtggt gttacccatc cccgccctcc tgcgcttgac gatgatcgga acgatgccct
     1801 tgcgcaacgc cagctcgcag gcgcgcagcg cctcgctctg ctccggcggc acccagacat
     1861 cgcgcttcac gtagccggcg gcgcgcatgc gttcgcggaa cgcgcgctgg tagtccgccg
     1921 aggttttctt ctgttcttcc atcgtgctga aggtccgccg actgcgtgcg gcgaggctgc
     1981 agcctcacct gtaaggatcg tagcaccggc aacggctgct atcgtagccg ggtcagaccg
     2041 tgcgctgccg gagacagccg gcacggaaag ctatagtgcg ggcgcgcacc gcgcgcgaaa
     2101 accccggggt gaatgttgtc atgggcctgc tcgattccct gttcaagctc ggcaagagcc
     2161 gcgtgcgcga agccgtcagc ggccgcaagc ccgagcgcac gctgccttcg gcgctcggtc
     2221 tgcgcatcgg cgcctcggtc gagttcgacc tgctgccggt gcgcatgaac gccggcgcct
     2281 tccgcttcac gctgccgcgc accgacgaac cgatgatcgt caccgcgcag ggccgcttcg
     2341 aactcggcga aggcatgatc gtgcaccgct tctacagcga cagttcgacg atgctgcaac
     2401 tgctctgccg cgacctgccc ggtctggagg accaggtcga ggagatcacg ctgtacgtgc
     2461 cgctggagag cgcctacccc gagaaccgcg agcagatcca tcgctgggtc gggccgcagg
     2521 ggcgcatcgg cgcgccggag ttccgcatcg ccgacggcac gcgctaccgg cgcatctggt
     2581 tcgacgacga gcccggcccg gcctcgccgg tgcgctacac cgaggaagtc ttcgacgaac
     2641 ccgacagctt cagccgcccg cgccgcatcg cccaggaggc catgctctac ggccgcgagg
//
LOCUS       DAOQJH010000419         2700 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_26552_length_2700_cov_3.046503, whole genome shotgun sequence.
ACCESSION   DAOQJH010000419 DAOQJH010000000
VERSION     DAOQJH010000419.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2700
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26552_length_2700_cov_3.046503"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..469)
                     /locus_tag="PLQ18_09695"
     CDS             complement(<1..469)
                     /locus_tag="PLQ18_09695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008291861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HNE01969.1"
                     /translation="MCGIIGIVGHGPVNQVIFDGLTVLQHRGQDAAGMMTCDGGRLYL
                     RKNNGLVRDVIRTRHMLRLLGSMGIGHVRYPTAGSSDSAESQPFYVNSPFGIAMAHNG
                     NLTNAHRLREELFRSDRRHLNTDSDSEVLLNVLAHELARNGRLSVEADDVFGAV"
     gene            complement(547..1059)
                     /locus_tag="PLQ18_09700"
     CDS             complement(547..1059)
                     /locus_tag="PLQ18_09700"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HNE01970.1"
                     /translation="MPQTMNWADYAILILIGLSMLLSLWRGFVREVISIVTWVLAFFL
                     AFNFSDLALAQLSHWVTLPETPSIRQLIGFATVFVGTLFVGGIVNLLIGQLVDGSGLG
                     PTDRMVGSLFGLARGVAIVALLVMLGSRTALVRDPWWQQSMLLPHFLPLAETLHSWLP
                     PEVASGVVFR"
     gene            complement(1383..2045)
                     /locus_tag="PLQ18_09705"
     CDS             complement(1383..2045)
                     /locus_tag="PLQ18_09705"
                     /inference="COORDINATES: protein motif:HMM:NF014207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HNE01971.1"
                     /translation="MQAAPVAAGLAEVLAAEAAPVDAGQSPSISGMPSVTTTPVAADA
                     GLNPAGVRGATASDAQAVPAPQAPLPVANPRAWAPLLGQHLMHLVQTGDTQATVRVNP
                     PQLGPVELRLELKDSLTHVSFYAHDAQVREALEQSLPRLRELLGAQGLQLGQSHVGDQ
                     ASQQQQQQPNPDGGSMAGSGDRRGNGRNDGMSGRDETAAVSGAEVVLPVRKLLGGVDH
                     YV"
     gene            2137..>2700
                     /locus_tag="PLQ18_09710"
     CDS             2137..>2700
                     /locus_tag="PLQ18_09710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE01972.1"
                     /translation="MAAGDTVMIGAAPVSCGSAAPGSGPAMSVAAAGLSAQMLAAATD
                     NTSTAPADGVDAGVVAGAVTALVGAAAAEGAVAAAGIPSIETAAVSGSVPPDACTSGI
                     DAAAPVGSAAPAAAPAVAALASPATGASGTATAAGTRKPSAAAMCACLVGAAAACGEA
                     SPVTDAAALPSSAAGASGPRPGKLPAVT"
BASE COUNT          419 a          960 c          944 g          377 t
ORIGIN      
        1 ggacggcgcc gaagacatca tcggcctcga cgctgagccg gccgttgcgc gccagctcgt
       61 gcgcgagcac gttcagcagc acctcggaat cggaatcggt gttcaggtgc cggcggtcgc
      121 tgcggaagag ttcctcgcgc agccggtggg cattggtcag gttgccgttg tgcgccatcg
      181 cgatgccgaa cggcgagttc acgtagaacg gctgcgattc ggccgaatcc gagctgccgg
      241 cggtcgggta gcgcacgtgg ccgatgccca tgctgccgag caggcgcagc atgtggcggg
      301 tgcggatcac gtcgcgcacc agaccgttgt tcttgcgcag gtacagccgg ccgccgtcgc
      361 aggtcatcat gccggcggcg tcctgacccc ggtgctgcag cacggtcagc ccgtcgaaga
      421 tcacctggtt gaccgggccg tgcccgacga tgccgatgat tccgcacatg gttgcttact
      481 ctcgggctgg gacggccgca cagccgccgg tggggggaat catcgatcgc tcggcgccgg
      541 cgctgcctag cggaacacga cgccgctcgc cacttccggc ggcagccagc tgtgcagggt
      601 ctcggcgagc ggcaggaagt gcggcagcag catcgactgc tgccaccacg gatcgcgcac
      661 cagcgccgtg cgcgagccga gcatcacaag cagcgccacg atcgccacgc cgcgcgccag
      721 cccgaacagc gaaccgacca tgcgatcggt cgggccgagg ccggaaccat cgaccagctg
      781 accgatcagc aggttgacga tgccaccgac gaacagcgtg ccgacgaaca cggtcgcgaa
      841 accgatcagc tgacggatcg acggcgtttc cggcagcgtc acccagtgcg acagctgcgc
      901 cagcgccaga tcggaaaaat tgaaggcgag gaagaaggcg agcacccagg tgacgatcga
      961 gatcacctcg cgcacgaagc cgcgccagag gctcagcagc atcgacaggc cgatcaggat
     1021 caggatggcg taatcggccc agttcatcgt ttgcggcacg cggagtttcc gtgccggctc
     1081 ggggctgccc ggcgagggcg cggattctat cagagcggcc ggcccgtcca aacccggatg
     1141 ccttcggggc catgcgcagc gatgtaaatc cggcacgaca gcgctgcagc ggcggcgttt
     1201 cggaccggca cagcgccatc ggcggcagga acggcagtcg tatcggcatc agcatccgaa
     1261 gattccggca acctcacgga ctgccgaaat gcgggtgcac cgcctgcccg acaagaccgg
     1321 tgccggaaac ccggccggca tggctgcgat gcaggccggt tcaggccgct tcagagagcg
     1381 gttcagacgt aatgatcgac accgccgagc agcttgcgca ccggcagcac gacttccgcg
     1441 ccgctcacgg cggccgtctc gtcgcgtccg ctcatcccgt cgtttcgtcc gttgccgcgc
     1501 cgatcgccgg agccggccat gctgccgccg tccggattcg gctgctgttg ctgctgctgc
     1561 gatgcctggt cgccgacgtg gctctggccg agctgcaggc cctgcgcgcc gagcagctcg
     1621 cgcagccgcg gcaggctctg ttcgagcgcc tcgcgcacct gcgcatcgtg ggcatagaag
     1681 ctgacgtggg tcagcgagtc cttcagctcc agacgcagct cgaccgggcc gagctgcggc
     1741 ggattgacgc gcacggtcgc ctgcgtgtcg ccggtctgca cgaggtgcat cagatgctgg
     1801 ccgagcagcg gtgcccaggc gcgcgggttg gcgaccggca gcggggcctg cggtgccggg
     1861 accgcctgcg cgtcgctggc ggtcgcaccg cgcacgccgg ccggattcag gccggcatcg
     1921 gcagcgaccg gtgtcgtcgt gaccgaaggc atcccggaaa tcgaaggcga ctgcccggcg
     1981 tcgaccggcg cggcttccgc ggccaggact tccgccagcc ccgccgccac cggcgcagcc
     2041 tgcacggatg ctccggctgc cgccagcgcc tgagcggcaa tgccggacgc tgtggcctgt
     2101 gacgccgacg gatcaccggc tggcgcggat cggcgcatgg cggccggcga caccgtgatg
     2161 atcggcgcag caccggtgtc ctgcggatca gcagcgcccg gcagcggccc ggcaatgtcg
     2221 gtggccgccg ccggactgtc ggcacagatg cttgcggcag ccacagacaa cacttcaacc
     2281 gcgccggctg atggcgtgga cgccggagtt gtcgcaggcg ctgtgaccgc actcgtcggc
     2341 gctgcagccg ccgagggcgc ggtggcagcc gccggcattc cgagcatcga gactgcggcg
     2401 gtttccggct ccgttcctcc cgacgcctgc acatccggca tcgatgcagc tgcgcccgtc
     2461 ggcagcgcag caccggctgc agcacccgcg gtcgccgctc tggcgtcgcc agccaccggc
     2521 gcctccggca ccgccacggc ggcgggcacc cgcaagcctt cggccgctgc catgtgtgcg
     2581 tgcctcgtcg gcgccgccgc ggcctgcggc gaagcctcgc cagtaaccga cgccgctgca
     2641 ctcccttcat ccgcagccgg cgcgtccggt ccgcggcccg gcaagctgcc ggcagtcacg
//
LOCUS       DAOQJH010000420         2687 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_26780_length_2687_cov_4.438070, whole genome shotgun sequence.
ACCESSION   DAOQJH010000420 DAOQJH010000000
VERSION     DAOQJH010000420.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2687
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26780_length_2687_cov_4.438070"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..763)
                     /locus_tag="PLQ18_09715"
     CDS             complement(<1..763)
                     /locus_tag="PLQ18_09715"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HNE01973.1"
                     /translation="MLSAPLPPDEAERLYALRSLDLLDTPREERFERITRLAVRLFGL
                     PIATVSLIDGDREWLKSVQGLALREIAREHSLAAHAILDNEPMIVSDAQADPRFGDYP
                     LVLGEPRFRFYAACPLHAGRRRHRVGTLSVAGRIAHRLAEADAQALRDLAAIAEAEIG
                     RSLASEVQSQLLRERDTARRQALLDALTGLWNRKAILDLLARELVRCEREGIGVSVVL
                     VDLDHFKAINDNHGHLTGDEALRAASAAMLQALRPY"
     gene            complement(874..2370)
                     /locus_tag="PLQ18_09720"
     CDS             complement(874..2370)
                     /locus_tag="PLQ18_09720"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNE01974.1"
                     /translation="MTKNPPETSRRNAFLHAHPWFVPQASAAELGSGQPVLLLDDTSG
                     LLRSVSAMLNAAGVPAVIGETDPQRLPARLSEQGCGVLLVDASLPELEPEVLLARLSA
                     EQPDLPVIVLTEAHDPEAAMRCLQAGAFDFLVKPVDRTRLLASVGRALEIRGLKHELA
                     SLTQTMLDDRLDHPEAFARLIAQDRSMGAACRYIEAIAPLSQPVLITGETGTGRKLLA
                     QALHAVSGRPGACVMVEVGGMDERRFAETLFGHRPGLHAEAGQPQEGRIGAAADGTLV
                     LLEVGELAPAAQQLLLNLLQERSFSPVGSGIVQECRARIVAISRHDLAHQADSGRLRK
                     DLYYRLRAHHVHVPPLRSRRGDVPLLVEHFVARAVEALGKGDVHIPAELYSLLRNHDF
                     PGNVAELEAMAYDAVASHRSGGTLGLASFRHCVSSTPDAPPPPPPVQSLSQLFPDRLP
                     TLKEAEAFLVAEALRRADNNQGIAAGLLGLTRQALNKRLVRGRQSSSH"
BASE COUNT          405 a          885 c          984 g          413 t
ORIGIN      
        1 cgtaggggcg cagcgcctgc agcatcgccg ccgaggcggc gcgcagcgcc tcgtcgccgg
       61 tcaggtggcc gtggttgtcg ttgatcgcct tgaagtgatc gagatcgacc agcacgacgc
      121 tgacgccgat gccctcgcgc tcgcagcgca cgagttcgcg cgcgagcagg tcgaggatgg
      181 ccttgcggtt ccagaggccg gtcagcgcgt cgagcagcgc ctgacgacgc gcggtgtcgc
      241 gctcgcgcag cagttgcgac tgcacctcgc tcgccagcga gcggccgatc tcggcctcgg
      301 cgatcgcggc cagatcgcgc agcgcctgcg catcggcctc ggccagccgg tgcgcaatcc
      361 ggccggcgac gctcagcgtg ccgacgcgat ggcgccggcg gccggcatgc agcggacagg
      421 ccgcgtagaa gcggaagcgc ggctcgccga gtaccagcgg atagtcgccg aagcgcggat
      481 cggcctgggc gtcgctgacg atcatcggtt cgttgtcgag gatggcgtgc gcggccagcg
      541 agtgctcgcg cgcaatctcg cgcagcgcca gtccctgcac ggacttcagc cattcgcgat
      601 caccgtcgat caggctgacc gtcgcgatcg gcagtccgaa caggcgcacg gccaggcggg
      661 ttatgcgctc gaaacgctcc tcgcgtggcg tatcgagcag gtccagcgag cgcagcgcgt
      721 acaggcgctc ggcttcgtct ggcggcagcg gggcggacag catgagcaat cacctgcggg
      781 tggcacgggg ggcatggccc cgaagcctgc ggtcctgcac gccgggcacg gcgtgcagtc
      841 cgcggatcgt tatttccggc cgccgggcct tgttcagtgg ctgctgctct ggcgcccgcg
      901 caccagtcgc ttgttcagcg cctgacgcgt caggccgagc aggccggccg cgatgccctg
      961 attattgtcg gcgcgacgca gcgcttcggc caccagaaag gcttcggctt ccttcagcgt
     1021 aggcagccgg tcggggaaaa gctgcgaaag actctgcacc ggtggtggtg gcggcggcgc
     1081 atcgggcgtg gaggacacgc agtggcggaa gctcgccagg ccgagcgtgc cgccgctgcg
     1141 gtggctggcg acggcgtcgt aggccatggc ttcgagttcg gcgacgttgc cggggaagtc
     1201 gtggttgcgc agcaggctgt agagctcggc aggaatgtgg acatcgccct tgccgagggc
     1261 ctcgaccgcg cgcgcgacga aatgctcgac cagcagcggc acgtcgccgc gccggctgcg
     1321 cagcggcggc acgtgcacgt ggtgggcacg cagccggtag tacagatcct tgcgcagccg
     1381 gccgctgtcg gcctggtgcg cgagatcatg acggctgatc gcgacgatgc gggcgcggca
     1441 ctcctgcacg atgccggagc cgaccgggct gaagctgcgt tcctgcagca ggttcagcag
     1501 cagttgctgc gcggccgggg caagctcacc gacttcgagc agtaccagcg taccgtcggc
     1561 cgccgcaccg atgcggcctt cctgcggctg gccggcttcg gcatgcaggc ccggacggtg
     1621 gccgaacagg gtttcggcga agcggcgctc gtccatgccg ccgacctcga ccatcacgca
     1681 ggcgccggga cggccggaaa cggcgtgcag ggcctgggcc agcagcttgc ggccggtgcc
     1741 ggtctcgccg gtgatcagca ccggctgcga cagcggcgcg atggcctcga tgtagcggca
     1801 ggcagccccc atgctgcggt cctgcgcaat cagccgggcg aaggcctcgg ggtgatcgag
     1861 ccggtcatcg agcatcgtct gggtcagcga ggccagctcg tgcttgagcc cgcggatctc
     1921 cagggcccgg ccgacgctcg ccagcaggcg cgtgcgatcg accggcttga ccagaaaatc
     1981 gaaggcgccg gcctgcaggc agcgcatcgc ggcctcggga tcatgggcct cggtcagcac
     2041 gatgaccggc aggtccggct gctcggcgct caggcgggcc agcaggactt cgggttcgag
     2101 ctcgggcagc gaggcatcga ccagcagtac gccgcagccc tgttcgctga gccgggccgg
     2161 caggcgctgc gggtcggttt caccgatcac ggccggcacg ccggcggcgt tgagcatggc
     2221 gctgaccgag cgcagcaggc cggaggtgtc gtcgagcagc agcacgggct gacccgaacc
     2281 gagctcggcg gcgcttgcct gtggcacgaa ccacggatgt gcatgcagga atgcattgcg
     2341 acgcgacgtt tccggggggt tcttcgtcat cgccggttct ccctcttgac cccagtacag
     2401 gggcatgtct cgtggattgc tatggacggc gagggttccg catcggttcg gttcttcgtc
     2461 atgctagctc cgatcatgta tgggtgccct gccctgggcg gatcgcaggc aggtcgccga
     2521 accgcggcgg tcccgctctg taggcgcaga tgccgcattc gcgccgggat gaccggagac
     2581 aacagcggca cctgcagact tcctgctcct ggaaaatcag gaccggaagg actggattac
     2641 gttcacgtat gcaaattttg gtccatgccc ggaatgacac acatccg
//
LOCUS       DAOQJH010000421         2675 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_26988_length_2675_cov_2.490458, whole genome shotgun sequence.
ACCESSION   DAOQJH010000421 DAOQJH010000000
VERSION     DAOQJH010000421.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2675)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2675)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2675
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26988_length_2675_cov_2.490458"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(59..463)
                     /locus_tag="PLQ18_09725"
     CDS             complement(59..463)
                     /locus_tag="PLQ18_09725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE01975.1"
                     /translation="MKHQKIILRLSLAALLLAVAGAAAARDYHHPPGYGWPVAPRGPT
                     STTLGGTLPQGRPMPRPGPYYTLPSPGGRTEIHDFSGQRYGTLERETGSRPPVVYAPD
                     GRRRYETDRDGTLRDPYGRRIEVLKPLPDGDW"
     gene            554..1738
                     /gene="dapE"
                     /locus_tag="PLQ18_09730"
     CDS             554..1738
                     /gene="dapE"
                     /locus_tag="PLQ18_09730"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011786013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HNE01976.1"
                     /translation="MSQAPASTTLALARELIARRSVTPADAGCQALMMERLAALGFEC
                     QRLRFGEVENFWARRGRAAPLFAFAGHTDVVPTGPLERWASDPFTPELRDGVLYGRGA
                     ADMKGSLAAMVTACERFIDAHPQHAGSIGFLITSDEEGPAIDGTVRVVDWLGARGEHI
                     DWCLVGEPSSAHTLGDTIKNGRRGSLHGHLTVHGVQGHVAYPHLVHNPIHALGPIIAV
                     LADEIWDHGNEFFPPTSFQISNVHGGAGATNVVPGTAELRFNFRFSTESTVEQLQERT
                     RLLVEMALLNEEVKAGWRFDATLEWELSGLPFLTEPGALVDAAVEAIYAETGTEAQLS
                     TSGGTSDGRFIAPTGAQVLELGPLNATIHKIDECVAADDLDALSRIYEGILQRLLARA
                     AG"
     gene            1840..>2675
                     /locus_tag="PLQ18_09735"
     CDS             1840..>2675
                     /locus_tag="PLQ18_09735"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HNE01977.1"
                     /translation="MSPSGWHILGAGAIGGLYATGLAAAGVPVTVLTRDTDSAAVLMR
                     EGLTVEEPDGHELHARPLAEPVVAGGASIRRLLVVTKAQDTVAALELIAHRLGPETRA
                     VLLQNGMGTADAVHLRFPRLMTVIGTTTNAAYRSTPRRLVRARRGETWIGDGPLPLPV
                     IERHILSEALAESGLHAAWDNDLKLRLWHKLVLNCAVNPVTALLDVPNGVLAGTGPAR
                     TLVAAVCIEAAAVMQAEGLPADARTLIEQVVRLAHESGRNVSSMRADVRAGRTTEIDF
                     ING"
BASE COUNT          376 a          987 c          926 g          386 t
ORIGIN      
        1 atgcgctgca tgacgtggcc tcctcgattc cggggcggcc accgtagcgc aggactcgct
       61 accagtcgcc gtccggcagc ggtttcagca cctcgatgcg ccggccgtag gggtcgcgca
      121 gcgtgccgtc gcgatcggtc tcgtagcgcc gccgcccgtc cggggcgtag acgaccggcg
      181 gacggctgcc ggtttcgcgt tcgagcgtgc cgtagcgctg gccgctgaaa tcatggattt
      241 cggtgcgccc gcccggcgag ggcagcgtgt agtacggccc gggccgcggc atcggccggc
      301 cctgcggcag cgtgccgccc agcgtcgtcg aggtcgggcc gcgcggtgcg accggccagc
      361 cgtagccggg cggatggtgg taatcgcggg cggccgcggc gccggcgacc gccagcagca
      421 gcgcggcgag gctcaggcgc aggatgatct tctggtgctt catgatcttg ccctccccgc
      481 cggtcgcagc cccgcggccg tggccggcag cacgtcgtac actacgccgc ttttcgaccc
      541 gccggagtcc ggaatgtccc aagcccccgc ctcgaccacg ctcgccctcg cgcgcgagct
      601 gatcgcccgc cgttcggtca cgcccgcgga tgccggctgt caggcgctga tgatggaacg
      661 cctcgccgcg ctcggtttcg agtgccagcg gctgcgcttc ggcgaagtcg agaacttctg
      721 ggcacgccgc ggtcgcgccg cgccgctgtt cgccttcgcc ggccataccg acgtggtgcc
      781 gaccggcccg ctcgaacgct gggcctccga tccgttcacg cccgagttgc gcgacggcgt
      841 gctgtacggg cgcggcgccg ccgacatgaa gggcagcctt gcagcgatgg tcacggcctg
      901 cgagcgcttc atcgatgcac atccgcagca cgccggctcg atcggcttcc tgatcaccag
      961 cgacgaggaa ggcccggcca tcgacggcac ggtgcgcgtc gtcgactggc tgggcgcgcg
     1021 cggcgagcac atcgactggt gtctggtcgg cgagccgtcc tcggcgcaca cgctcggcga
     1081 cacgatcaag aacggccggc gcggctcgct gcacgggcat ctgaccgtgc acggcgtgca
     1141 gggccatgtc gcctatccgc atctggtgca caacccgatc cacgcgctcg gaccgatcat
     1201 cgccgtgctt gccgacgaaa tctgggacca cggcaacgag ttcttcccgc cgacctcgtt
     1261 ccagatctcc aacgtccacg gcggcgccgg cgccaccaac gtcgtgccgg gcacggcgga
     1321 gctgcgcttc aacttccgct tctcgaccga atcgacggtc gagcagctgc aggagcgcac
     1381 gcggctgctg gtcgagatgg cgctgctgaa cgaggaggtc aaggccggct ggcgcttcga
     1441 tgcgacgctc gaatgggaac tctccggcct gcccttcctg accgagccgg gcgcgctggt
     1501 cgatgccgcc gtcgaggcga tctacgccga gaccggcacc gaggcgcagc tctcgaccag
     1561 cggcggcacc tcggacggcc gcttcatcgc cccgaccggt gcgcaggtgc tcgaactcgg
     1621 gccgctgaac gcgacgatcc acaagatcga cgaatgcgtc gccgccgatg atctcgatgc
     1681 actgtcgcgc atctacgaag gcatcctgca gcgcctgctg gcgcgcgccg ccggctgaga
     1741 caccccgccc gccgggagcg gcagccccgc acaagccggt tcacaagccc cggcctgacc
     1801 gctaccctgc cgctctttgc gcagggggac cgtaacacga tgtcaccttc cggctggcac
     1861 atcctcggcg ccggcgccat tggcgggctg tacgcgaccg ggctcgccgc tgccggcgtg
     1921 ccggtgacgg tgctgacgcg cgacaccgac agtgccgcgg tgctgatgcg cgagggcctg
     1981 acggtcgagg agcccgacgg ccacgagctg cacgcccgcc cgctcgccga gccggtcgtc
     2041 gccggcggcg cgagcatccg ccggctgctg gtggtgacca aggcgcagga cacggtcgcc
     2101 gcgctcgaac tgatcgccca ccgcctcggc cccgaaaccc gcgccgtgct gctgcaaaac
     2161 ggcatgggca cggccgatgc cgtgcacctg cgctttccgc ggctgatgac cgtgatcggc
     2221 accacgacca acgccgccta ccgcagcacc ccgcggcgac tggtgcgcgc acgtcgcggc
     2281 gaaacctgga tcggtgacgg cccgctgccg ctgccggtga tcgagcggca catcctcagc
     2341 gaggcgctgg ccgaatccgg cctgcacgcc gcctgggaca atgacctgaa gctgcggctc
     2401 tggcacaagc tcgtgctgaa ctgcgcggtc aacccggtca cggcgctgct cgacgtgccg
     2461 aacggcgtgc ttgccgggac cggtccggcg cgcacgctgg tggcggcggt ctgcatcgag
     2521 gctgccgcgg tcatgcaggc cgagggcctg ccggccgatg cgcgcacgct gatcgagcag
     2581 gtcgttcgcc tcgcgcacga aagcgggcgc aatgtgtcct cgatgcgcgc cgacgtgcgc
     2641 gccggccgca ccaccgaaat cgatttcatc aacgg
//
LOCUS       DAOQJH010000422         2671 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_27047_length_2671_cov_4.034404, whole genome shotgun sequence.
ACCESSION   DAOQJH010000422 DAOQJH010000000
VERSION     DAOQJH010000422.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2671)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2671)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2671
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27047_length_2671_cov_4.034404"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            26..1654
                     /locus_tag="PLQ18_09740"
     CDS             26..1654
                     /locus_tag="PLQ18_09740"
                     /inference="COORDINATES: protein motif:HMM:TIGR00831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HNE01978.1"
                     /translation="MSLPLAILVLLFAVLACGAVSRRSAMPLPLLLIPAGTLLALVPG
                     MHAVAPDPETFFLVFVPPLLFADAWLIPKREFLNLRYPILGLALGLVLCTVLATGYLV
                     HWLIPSVPLAAGFALGAVISPTDAVAVSSITERLRLPARLTHLLNGESLINDASGLVA
                     FRFSVAAALTGSFSAGAAAANFMLVAVGGVLAGLAATWVLGRLRRGLSHRGWADESIS
                     TGLTLLAPYASYLAAESLHLSGVLGAVAGGWYAAMQEARDTKPSERLVMLNVWQLLLF
                     FFNGLVFVLLGLRLPEVLEGVAGQPWPMLAGGALAITAVAMLIRLVWVFPASRAMLWL
                     TRRHAPQTPVPPWRQVLVAGWAAPRGAITLAAALSLPAGGFPERELLVFYASAVIVLT
                     LVVNALTLPALIRRLGLRADDVQLREMRSAHVALAQAAIRRLRAALDLQHAEHEMRYT
                     QRLIEDYERRIGRHCGRSAEEAMRIAPEFETEISLRRLGIAAERRELRRLRREALISD
                     EVIRDLLHDLDLREVGLDSVARQPSAASARAAEA"
     gene            1702..2664
                     /gene="arsS"
                     /locus_tag="PLQ18_09745"
     CDS             1702..2664
                     /gene="arsS"
                     /locus_tag="PLQ18_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767285.1"
                     /note="Some members of this family are selenoproteins;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenosugar biosynthesis radical SAM protein
                     ArsS"
                     /protein_id="HNE01979.1"
                     /translation="MHATRPLLDAIDFPALRRRRLETVQANLGYRCNQACLHCHVNAG
                     PTRREAMSRDTAEAVLELVAASGATTLDLTGGAPELNPHFRWLVETAHARGVQVMDRC
                     NLTILSEPGHEDLAGFLAAHRVEVVASLPCYLEDNVDGQRGAGVYARSIAGLRQLNAL
                     GYGDGRSGLMLNLVYNPTGPYLPPPQAALEADYRRELGGRHGIVFDRLFTLANMPIQR
                     FGSTLVSHGEFDAYMTLLRGAHRDDNLDQVMCRTLVSIGWDGTVYDCDFNQMLGLPLA
                     VPGRTRPQVRDLIGRDLAGNPIAVAGHCYGCTAGQGSSCGGALG"
BASE COUNT          310 a          973 c          958 g          430 t
ORIGIN      
        1 tccccccgtc cggagtcccg cctcgatgag cctgccgctc gcgatcctcg tgctgctgtt
       61 cgccgtgctt gcctgcggtg cggtgtcgcg gcgcagcgcc atgccgctgc cgctgctgct
      121 gatcccggcc ggcacgctgc tggcgctggt gccgggcatg cacgcggtcg cgcccgatcc
      181 cgagaccttc tttctggtgt tcgtgccgcc gctgctgttc gccgatgcct ggctgatccc
      241 gaagcgcgag ttcctgaacc tgcgctatcc gatcctcggg ctggcgctcg ggctcgtgct
      301 ctgcacggtg ctcgcgaccg gctacctcgt gcactggctg atcccgtcgg tgccgctcgc
      361 ggccggcttc gcgctcgggg cggtgatttc gccgaccgat gcggtcgcgg tgtcgtcgat
      421 caccgagcgg ctgcggctgc cggcgcggct gacgcatctc ctcaacggcg aaagcctgat
      481 caacgatgcc tcggggctgg tggcgttccg cttctcggtc gccgcggcgc tgaccggcag
      541 cttctcggcc ggcgcggcgg cggcgaactt catgctggtc gccgtcggcg gtgtgctcgc
      601 cgggctggcg gcgacctggg tgctcgggcg gctgcggcgc gggctgtcgc accgcggctg
      661 ggccgatgaa tcgatctcga ccggcctgac gctgctggcg ccgtatgcgt cctatctggc
      721 ggccgaatcg ctgcatctgt ccggcgtgct cggcgcggtc gccggcggct ggtacgcagc
      781 gatgcaggag gcgcgcgaca ccaagccgtc cgagcggctg gtcatgctca acgtctggca
      841 gctgctgctg ttcttcttca acggtctggt gttcgtgctg ctcggcctgc gcctgcccga
      901 agtgctcgaa ggcgtcgccg gccagccatg gccgatgctt gccggcggtg cgctggcgat
      961 cacggccgtg gcgatgctga tccggctggt ctgggtgttc ccggcctcgc gcgcgatgct
     1021 gtggctgacg cgccggcacg cgccgcagac gccggtgccg ccgtggcggc aggtgctggt
     1081 ggccggctgg gcagccccgc gcggggcgat cacgctggcg gcggcgctgt cgctgccggc
     1141 cggcggcttt ccggagcgcg agctgctggt gttctacgcg agcgcggtga tcgtgctgac
     1201 gctggtcgtc aacgcgctga cgctgccggc gctgatccgc cggctcggcc tgcgcgccga
     1261 cgacgtgcag ttgcgcgaga tgcgcagcgc ccacgtcgcg ctggcgcagg cggcgatccg
     1321 gcggctgcgc gccgcgctcg acctgcagca cgccgagcac gagatgcgct acacgcagcg
     1381 gctgatcgag gactacgagc ggcgcatcgg ccgccactgc ggtcgctcgg ccgaggaggc
     1441 gatgcgcatc gcgccggagt tcgagaccga gatcagcctg cgccggctcg gcatcgccgc
     1501 cgagcggcgc gagctgcgcc ggctgcggcg cgaggcgctg atcagcgacg aggtcatccg
     1561 cgatctgctg cacgatctcg acctgcgcga ggtcggcctc gacagcgtcg cccgccagcc
     1621 ctcggcggca tccgcgcgcg ccgccgaagc gtaaagtcag gctccccccg ccgccggaac
     1681 gcattccagg gacccgccgc catgcacgcc acgcgcccgc tgctcgatgc catcgatttc
     1741 ccggcgctgc gccgccgccg gctcgaaacc gtgcaggcca acctcggcta ccgctgcaac
     1801 caggcctgcc tgcactgcca cgtcaacgcc ggcccgacgc ggcgcgaggc aatgtcgcgc
     1861 gacaccgccg aagccgtgct cgaactcgtc gccgcgagcg gcgcgacgac gctcgacctg
     1921 accggcggcg cgccggagct gaatccgcac ttccgctggc tggtcgaaac cgcccatgcg
     1981 cgcggcgtgc aggtgatgga tcgctgcaac ctgaccatcc tgtccgagcc cggccacgag
     2041 gatctggcgg gctttctcgc cgcgcaccgg gtcgaggtcg tcgcctcgct gccgtgctac
     2101 ctcgaagaca acgtcgacgg tcagcgcggc gccggggtgt atgcgcgcag catcgccggc
     2161 ctgcggcagt tgaacgcgct cggctacggc gacggccgtt ccgggctgat gctgaatctg
     2221 gtctacaacc cgaccggtcc gtacctgccg ccgccgcagg ccgcgctcga agccgactac
     2281 cggcgcgaac tcggcgggcg ccacggcatc gtcttcgacc ggctgttcac gctcgccaac
     2341 atgccgatcc agcgcttcgg ctcgacgctg gtctcgcacg gcgagttcga cgcctacatg
     2401 acgctgctgc gcggtgcgca ccgcgacgac aacctcgatc aggtcatgtg ccggacactg
     2461 gtgtcgatcg gctgggacgg cacggtctac gactgcgact tcaaccagat gctcggcctg
     2521 ccgctcgccg tgccgggccg cacgcgcccg caggtgcgcg atctgatcgg ccgcgatctc
     2581 gccggtaacc cgatcgcggt ggccggtcac tgctacggct gcacggccgg gcaggggtcg
     2641 agttgcggcg gggcgctggg ctgagcgcgg g
//
LOCUS       DAOQJH010000423         2665 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_27152_length_2665_cov_2.741762, whole genome shotgun sequence.
ACCESSION   DAOQJH010000423 DAOQJH010000000
VERSION     DAOQJH010000423.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2665
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27152_length_2665_cov_2.741762"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(30..818)
                     /locus_tag="PLQ18_09750"
     CDS             complement(30..818)
                     /locus_tag="PLQ18_09750"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018719444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HNE01980.1"
                     /translation="MDGGWLRPLLFAGVLLALLALESRAPRRAWTQPRWPRVARHLAL
                     VGLDAVLLRLTLPLLATGAAALAEARGWGLLRALAVPAPLAGMLSLLLLDLAIWAQHV
                     AMHRVPLLWRLHRVHHTDTDFDTSTALRFHPLEIGLSMLWKIVLVLALGAPVAAVAGF
                     ELLLSACALFNHANLALPAPVDAWLRRLLITPDVHRIHHSIHRDETDSNFGFSVVWWD
                     HLFGTWRSAPRDGHATMTIGLDGFRAPQAQRLGALLLQPLRRDG"
     gene            complement(822..>2665)
                     /locus_tag="PLQ18_09755"
     CDS             complement(822..>2665)
                     /locus_tag="PLQ18_09755"
                     /inference="COORDINATES: protein motif:HMM:NF021606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HNE01981.1"
                     /translation="VIGTLWGGLRYRPELLASTAPFLLAFFGLFSLLPVLAAHRLPPD
                     PKRPLDAGLVFGVPVLTLALQAALLEGVEHGLALSALGCAAWYAALWRFCRQRPALAL
                     LGECQAAIALVSATLAVPLYFEAGTSSPLWALEGAGLVWVGARQDRRGLTGFGALLLF
                     AAGALWLDAHLPDMPVSRPFLRADALDTPLLALAALFAAAALGRLRPAPQPLRLETVL
                     AGWGAFWWLLWCLRETAMSTLAVDAASGVWLLFLAASSALAFLAWRRLALGWLRVPLY
                     GLPLLLAPLAMHDFLHRDHPLAGLGAAGWLAAFAVQFGLLRVESSAADAGDRGRRWRY
                     AAAVWLLLALALQEVAWLIGDVVHLAFVWTRAANGVLIAATLALLLDGERPAWPLAAY
                     GTAGRRLAGAPVALLLLAWFPAAGLHGPALPAPLPFVPLLNPVELAAVAALLVLARAG
                     GRPWFVPPAQLPAARTVLAALGFATLNLAVLRACHHLAGVPWSAAALWDSRLVQTALA
                     LVWATAAVVLMLTGTRRGQRGIWLAGAGLMALVVLKLFVVDLDGRDTLARILAFLGTG
                     TLLLLVGWLAPVPPRAAPGPSADTDADTESGPSPAPAAAPVDAPPAS"
BASE COUNT          439 a          996 c         1013 g          217 t
ORIGIN      
        1 gtggcggggg gcagcgggtc agcgcaggct cagccgtcgc ggcgcagcgg ctgcagcagc
       61 agcgcgccga ggcgctgggc ttgcggggcg cggaagccgt cgaggccgat cgtcatcgtc
      121 gcgtgtccgt cgcgcggtgc cgagcgccag gtgccgaaca ggtgatccca ccagacgacg
      181 ctgaagccga agttgctgtc ggtctcgtcg cggtggatcg agtggtggat gcggtgcacg
      241 tccggcgtga tcagcaggcg gcgcagccac gcatcgaccg gcgccggcag cgcgaggttg
      301 gcgtggttga acagcgcaca ggccgacagc agcagctcga agcccgcaac cgccgcgacc
      361 ggcgcaccga gcgcgagcac cagcacgatc ttccagagca tcgacagccc gatctccagc
      421 gggtgaaagc gcagcgccgt gctggtgtcg aagtcggtgt cggtgtgatg cacgcggtgc
      481 aggcgccaga gcagcggcac gcggtgcatc gcgacatgct gcgcccagat cgccagatcg
      541 agcagcagca ggctcagcat cccggcgagc ggcgccggca cggcgagcgc ccgcagcagg
      601 ccccagccgc gtgcctcggc gagcgccgcg gccccggtcg cgagcagcgg cagcgtgagg
      661 cgcagcagca cggcatcgag cccgaccagc gccaggtggc gtgcgacgcg cggccagcgc
      721 ggctgcgtcc aggcgcgacg cggcgcgcgc gattcgagcg ccagcagcgc gagcagcacg
      781 ccggcgaaca gcagcggacg cagccagcca ccgtccatcg atcagctcgc cggcggcgcg
      841 tcgaccggcg ccgcggcagg ggcgggggat ggcccgcttt cggtgtcggc gtcggtgtcg
      901 gcggacggtc cgggcgcggc gcgcggcggc accggtgcca gccagccgac cagcagcagc
      961 agggtgccgg tgccgagaaa ggcgaggatg cgcgccagcg tgtcgcgacc gtccagatcg
     1021 acgacgaaga gcttcagcac caccagcgcc atcagcccgg cgccggcgag ccagatgccg
     1081 cgctgcccgc ggcgcgtgcc ggtgagcatc agcacgaccg ccgccgtcgc ccagaccagc
     1141 gccagcgcgg tctgcacgag gcgcgaatcc cacagcgcag cggccgacca cggcacgccg
     1201 gcgaggtgat ggcaggcgcg cagcacggcg aggttcagcg tcgcgaagcc gagcgccgcc
     1261 agcacggtgc gcgcggccgg cagttgcgcc ggcggcacga accacggtcg gccgccggcg
     1321 cgcgcgagca ccagcagtgc ggcgacagca gccagttcga ccggattcag cagcggcacg
     1381 aacggcagcg gcgccggcag cgccgggccg tgcaggccgg cggccgggaa ccacgcgagc
     1441 agcagcagcg ccaccggtgc gccggcgagc cggcgaccgg ccgtgccgta cgcggcgagc
     1501 ggccacgccg ggcgctcgcc atcgagcagc agcgccagcg tcgccgcgat cagcacgccg
     1561 ttggcggcgc gcgtccacac gaaagcgaga tgcacgacat cgccgatcag ccacgccacc
     1621 tcctgcagcg ccagcgccag cagcagccag acggcggccg cgtagcgcca gcgccggccg
     1681 cgatcaccgg catcggccgc ggaggattcg acgcgcagca gcccgaactg cacggcgaac
     1741 gccgccagcc agccggcagc gccgaggccg gcgagcgggt ggtcgcggtg caggaagtcg
     1801 tgcatggcca gcggcgcgag cagcagcggc agcccgtaca gcggcacgcg cagccagccg
     1861 agcgccagcc gccgccaggc gaggaaggcg agcgcgctgc tcgcggcaag gaacagcagc
     1921 cagacgccgc tcgcagcatc gaccgccagc gtcgacatcg ccgtctcgcg caggcaccac
     1981 agcagccacc agaacgcccc ccagccggcg agcacggttt cgagccgcag cggctgcggc
     2041 gccgggcgca ggcgcccgag ggcggcggcg gcgaacagcg ccgcgagcgc gagcagcggc
     2101 gtgtcgagcg cgtcggcgcg caggaacggc cgcgacaccg gcatatcggg caggtgcgcg
     2161 tccagccaca gcgccccggc ggcgaacagc agcagcgcgc cgaagccggt cagcccgcgc
     2221 cggtcctgcc gggcgccgac ccagaccagc ccggcacctt cgagcgccca cagcgggctc
     2281 gacgtgccgg cctcgaagta cagcggcacg gcgagcgtcg ccgacaccag cgcgatcgct
     2341 gcctgacatt cgccgagcag cgcgagcgcc ggccgctgcc ggcagaagcg ccagagcgcc
     2401 gcataccagg ccgcgcagcc gagtgcgctc agcgccagtc cgtgttcgac gccttcgagc
     2461 agcgccgcct gcagcgccag cgtcagcacc ggcacgccga acaccagccc ggcgtcgagc
     2521 gggcgcttcg ggtccggcgg cagccggtgc gcggccagca ccggcagcag cgagaacagc
     2581 ccgaagaacg cgagcaggaa gggcgcggtg ctcgccagca gctccggccg gtagcgcagc
     2641 ccgccccaca gcgtgccgat cacgt
//
LOCUS       DAOQJH010000424         2664 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_27172_length_2664_cov_2.392871, whole genome shotgun sequence.
ACCESSION   DAOQJH010000424 DAOQJH010000000
VERSION     DAOQJH010000424.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2664)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2664)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2664
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27172_length_2664_cov_2.392871"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1257
                     /locus_tag="PLQ18_09760"
     CDS             <1..1257
                     /locus_tag="PLQ18_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012696582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNE01982.1"
                     /translation="ARSTAAAVELLESTGIPEPRRRAAAFPHQLSGGQRQRAMIAMAL
                     ACRPKLLIADEPTTALDVTLQVQVLDLLRELQAEFGMAILFISHDLNLVRRFAGRVCV
                     MHRGRIVEQGPVEAVFTAPREPYTRTLLASRPQRLIPDGEHPAQSGAEALLSARGVDV
                     RFVTRRDWLGRPRAAVHALVATDLDVHAGETLGIVGESGSGKSTLGLALLRLLPAAGR
                     IVVDGQEWSGLGQRRLRPLRPAFQVVFQDPFGSLSPRMTVEDIVGEGLRIHRPALDRA
                     QRRARIVAALDEVGLPETALWRYPHEFSGGQRQRIAIARALVLEPKLLLLDEPTSALD
                     ATVQKQVLALLRTLQQRHGMSYLFISHDLAVIRALAHRVLVLRGGQVVESGETAALFS
                     APQHPYTAALLEAALLPGAGAPAPAG"
     gene            complement(1305..>2664)
                     /locus_tag="PLQ18_09765"
     CDS             complement(1305..>2664)
                     /locus_tag="PLQ18_09765"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HNE01983.1"
                     /translation="QRPAPADFAATTDDGDLWQRLRGQLSFADTSHPDVQRQLQSLRP
                     RSIASVADNASNYLYFVLDATQSRGMPAEVALVPWFESGYRPNAVSPGRAAGLWQLMP
                     DTATHLGLSLGRGVDERKDVKASTEAALDYLARLNRNFDGDWLLTLAAYNAGPKTVQN
                     AIERNRRAGRPTAFWDLDLPRITQEYVPRILALSQMIASPERFGLNLPDAGENSALTH
                     VDLGPGVNPMLAAAAAGLSREELLRYNARLRGRDDTGSTELLLPKARAEALARVRPRI
                     EGTKLAVWEGYRLAPGDTIEAIAERFDTSPAALREVNREAGATLSFTAGREVLVPARG
                     PVRLTMAAAPAQRPKPQTGRLARAKKPGKPEPRPAEAGDGLFAGDVPLRERIRRLLTI
                     NLTVRPGDSLAVLSGQYSVAIEDLRRWNDLPASTPAGEPLEAGRTVVVYVDAIADDIP
                     RG"
BASE COUNT          329 a          947 c         1015 g          373 t
ORIGIN      
        1 gcccgcagta cggccgccgc ggtcgaactg ctggagagca ccggcatccc ggagccgcgg
       61 cgccgggccg ccgccttccc gcaccagctc tccggcggcc agcgccagcg ggcgatgatc
      121 gcgatggcgc tggcctgccg gccgaagctg ctgatcgccg atgagccgac cacggcgctc
      181 gacgtgacgc tgcaggtgca ggtgctcgac ctgctgcgcg aactgcaggc cgagttcggc
      241 atggcgatcc tgttcatcag ccacgacctg aacctggtgc gccgcttcgc cgggcgcgtc
      301 tgcgtcatgc accgcggccg catcgtcgag caggggccgg tcgaggcggt gttcacggcg
      361 ccgcgcgagc cgtatacgcg cacgctgctc gcgagccgcc cgcagcggct gatcccggac
      421 ggcgaacatc cggcgcaaag cggcgccgaa gcgctgctca gcgcgcgcgg cgtcgacgtg
      481 cgcttcgtga cgcgccgcga ctggctcggc cggccgcgcg ccgcggtgca tgcgctggtt
      541 gcgaccgatc tcgacgtgca tgcgggcgag acgctcggca tcgtcggcga gagcggttcg
      601 ggcaagtcga cgctcgggct cgcgctgctg cggctgctgc cggctgccgg gcgcatcgtc
      661 gtcgacgggc aggaatggag cggactcggc cagcgccggc tgcggccgct gcgcccggcc
      721 ttccaggtcg tgttccagga cccgttcggc tcgctgtcgc cgcgcatgac ggtggaggac
      781 atcgtcggcg agggcctgcg catccaccgc ccggcgctcg atcgcgcgca gcgccgtgcc
      841 cgcatcgtcg ctgcgctcga cgaggtcggc ctgccggaaa ccgcgctgtg gcgctatccg
      901 cacgagttct cgggcggcca gcgccagcgc atcgcgatcg cacgggcgct ggtgctggag
      961 ccgaagctgc tgctgctcga tgagccgacc agcgcgctcg atgcgacggt gcagaagcag
     1021 gtgctggcac tgctgcgcac gctgcagcag cgccacggca tgagctacct gttcatcagc
     1081 cacgacctgg cggtgatccg cgcgctcgcg caccgcgtgc tggtgctgcg cggcggtcag
     1141 gtcgtggagt ccggcgagac cgcggcgctg ttctcggcgc cgcagcaccc gtacacggcc
     1201 gcgctgctcg aagccgcgct gctgccgggc gccggtgcac cggcgccggc cggctgaagc
     1261 cgggacggga agccccccgg ccggcgcgaa actcttggcc ggcttcagcc gcgcgggatg
     1321 tcgtcggcga tcgcgtcgac gtagacgacg acggtgcggc cggcttcgag cggctcgccc
     1381 gccggcgtcg aggccggcag atcgttccag cggcgcaggt cctcgatcgc gacgctgtat
     1441 tgccccgaga gcacggccag cgaatcgccg gggcgcacgg tcaggttgat cgtcagcagc
     1501 cggcggatgc gctcgcgcag cggtacatcg ccggcgaaca ggccgtcgcc ggcttccgcc
     1561 ggccggggct ccggcttgcc gggctttttc gcgcgcgcaa gccgcccggt ctggggcttc
     1621 ggccgctgcg ccggggctgc ggccatggtc aggcgcaccg ggccgcgcgc cggcaccagc
     1681 acctcgcgtc cggccgtgaa gctcagcgtc gcaccggcct cgcggttgac ctcgcgcagc
     1741 gccgcagggc tggtgtcgaa gcgctcggcg attgcctcga tcgtgtcgcc cggcgccagc
     1801 cggtagcctt cccagaccgc gagcttcgtg ccctcgatgc gcgggcgcac gcgcgccagc
     1861 gcctcggccc gggccttcgg cagcagcagc tcggtgctgc cggtgtcgtc gcgaccgcgc
     1921 agccgggcgt tgtagcgcag cagttcctcg cggctcaggc cggcggcggc ggcggcgagc
     1981 atcgggttga cgccgggacc gaggtcgaca tgggtcagcg ccgagttctc gccggcatcc
     2041 ggcaggttca ggccgaagcg ctcggggctc gcgatcatct gcgacagcgc caggatgcgc
     2101 ggcacgtact cctgcgtgat gcgcggcagg tcgaggtccc agaacgcggt cgggcggccg
     2161 gcgcggcggt tgcgctcgat ggcgttctgc acggtcttcg gaccggcgtt gtaggcggcg
     2221 agcgtcagca gccagtcgcc gtcgaagttg cggttcaggc gcgccaggta atcgagcgcg
     2281 gcctcggtcg aggccttgac gtccttgcgc tcgtcgacgc cgcgaccgag gctcaggccg
     2341 agatgcgtgg cggtatcggg catcagctgc cagaggccgg cggcgcggcc cggcgacacc
     2401 gcgttcggcc ggtagccgct ctcgaaccac ggcaccagcg cgacctcggc cggcatgccg
     2461 cggctctgcg tggcatcgag cacgaagtac aggtagttcg aggcgttgtc ggccaccgag
     2521 gcgatgctgc gcgggcgcag cgactgcagc tggcgctgca cgtccgggtg cgaggtgtcg
     2581 gcaaaggaca gctggccgcg cagccgctgc cagagatcac cgtcatcggt ggtggcggcg
     2641 aaatcggcgg gggcggggcg ctgg
//
LOCUS       DAOQJH010000425         2662 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_27204_length_2662_cov_4.914845, whole genome shotgun sequence.
ACCESSION   DAOQJH010000425 DAOQJH010000000
VERSION     DAOQJH010000425.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2662
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27204_length_2662_cov_4.914845"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1177)
                     /locus_tag="PLQ18_09770"
     CDS             complement(<1..1177)
                     /locus_tag="PLQ18_09770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008114767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HNE01984.1"
                     /translation="MTMNDPLTPPRRWDTQSQNRAARLGRAAAFARGRHVAAADARAL
                     LEAVLEPGDRVCLEGNNQKQADFLAKTLVTVDPARVHDLHMVQSVLALPEHIDVFERG
                     IATKLDFSFSGPQGARLAKLVSAGRIDIGAIHTYLELFGRYFTDLTPRVSLIAAQAAD
                     AAGNLYTGPNTEDTPVIVEATAFKRGIVIAQVNEVVDTLPRIDIPADWVDFVIPAPTP
                     AYVEPLFTRDPAQISEVQVLMAMMAIQGIYAEYGVQRLNHGIGFDTAAIELLLPTYGE
                     SLGLKGKICRHWALNPHPALIPAIEAGWVESVHSFGGELGMEAYTAARPDVFFTGPDG
                     SMRSNRALCQAAGHYACDLFIGSTLQIDLAGNSSTATLGRIAGFGGAPNMGADARGRR
                     "
     gene            complement(1323..2087)
                     /gene="madM"
                     /locus_tag="PLQ18_09775"
     CDS             complement(1323..2087)
                     /gene="madM"
                     /locus_tag="PLQ18_09775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018706711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005342 - organic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015849 - organic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate transporter subunit MadM"
                     /protein_id="HNE01985.1"
                     /translation="MYETLETLLGKNGLLTAFAVVGATMWLSYWLSDRLTRGRIHGSA
                     IAILAGLVLAYVGGVAAGGKNGLADLKLFSGLGLMGGAMLRDFAIVATAFGVSFDELK
                     KCGLSGVLSLLVGVVVSFVLGAAVAYGFGYTDAVSMTTIGAGAVTYIVGPVTGAAIGA
                     SSEVIALSIAAGLVKSVLVMIGTPFVAKWIGLDNPRTAMIFGGMMGTTSGVCGGLAAT
                     DPKLVPYGAMTSTFYTGLGCLMGPSVLFLATRAMFG"
     gene            complement(2093..2512)
                     /gene="madL"
                     /locus_tag="PLQ18_09780"
     CDS             complement(2093..2512)
                     /gene="madL"
                     /locus_tag="PLQ18_09780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007158540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005342 - organic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015849 - organic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate transporter subunit MadL"
                     /protein_id="HNE01986.1"
                     /translation="MVIYGVALLSICTLIGMFAGDLLGQLIGVKANVGGVGFAMLLLI
                     LISDRLRRDLKLVPMSQRGIEFWSAMYIPIVIAMASTQNVAAAIKGGPAALLAGVIAT
                     IACAALIPVLDRWGRPKPAVSGNSASLSTTVIASGER"
BASE COUNT          431 a          868 c          966 g          397 t
ORIGIN      
        1 gccggcggcc gcgtgcatcg gcgcccatgt tcggcgcgcc gccgaagccg gcgatgcgcc
       61 cgagtgtcgc ggtcgagctg ttgccggcga gatcgatctg cagcgtcgag ccgatgaaca
      121 gatcacaggc gtagtggccg gcggcctgac acagcgcgcg gttcgaacgc atgctgccgt
      181 ccgggccggt gaagaacacg tcggggcgcg ccgccgtgta ggcctccatg ccgagttcgc
      241 cgccgaagga atgcacggac tccacccagc cggcctcgat cgccgggatc agcgccggat
      301 gcgggttcag cgcccagtgc cggcagattt tgcccttcag gcccagcgac tcgccgtagg
      361 tcggcagcag cagctcgatc gccgccgtat cgaagccgat gccgtggttc aggcgctgca
      421 cgccgtattc ggcgtagatg ccctggatgg ccatcatcgc catcagcacc tgcacctcgc
      481 tgatctgcgc cggatcgcgc gtgaacagcg gctcgacgta ggccggggtc ggcgccggga
      541 tcacgaagtc cacccagtcg gccgggatgt cgatgcgcgg cagcgtgtcg acgacctcgt
      601 tgacctgcgc gatgacgatg ccgcgcttga aggccgtcgc ctcgacgatc accggcgtgt
      661 cctcggtgtt cgggccggtg tagaggttgc cggcggcatc ggcggcctgc gcggcgatca
      721 ggctgacgcg cggggtgagg tccgtgaagt agcggccgaa cagttcgagg taggtgtgga
      781 tcgcgccgat gtcgatgcgc ccggcgctga cgagcttcgc gagccgcgcg ccctgcgggc
      841 cggagaacga gaagtcgagc ttcgtcgcga tgccgcgctc gaacacgtcg atgtgctcgg
      901 gcagcgccag caccgactgc accatgtgca ggtcgtgcac gcgcgccggg tcgaccgtga
      961 ccagcgtctt cgcgaggaag tcggcctgct tctggttgtt gccttcgagg cagacgcggt
     1021 cgccgggctc cagcacggct tcgagcagcg cgcgggcatc ggcggcggcg acgtgccggc
     1081 cgcgggcgaa agcggcggcg cgtccgaggc gggcggcgcg gttctgcgat tgcgtgtccc
     1141 agcggcgcgg gggggtgagg gggtcattca tggtcatcgc actccttgga gcagggcagg
     1201 ggatcggggt ggtgcctgcc ggtgcgatcc gtccggtttc cgtgatgaca agatgatctt
     1261 aataaaatta tcttgaaatt acggacacga agggggacgg accggcaccg tgccggcagc
     1321 tttcagccga acatcgcccg cgtggcgagg aacagcaccg acgggcccat caggcagccg
     1381 aggccggtgt agaaggtcga ggtcatcgcg ccgtagggca cgagcttcgg atcggtcgcg
     1441 gccagaccgc cgcagacgcc gctggtcgtg cccatcatgc cgccgaagat catcgcggtc
     1501 cgcgggttgt cgaggccgat ccacttcgcg acgaagggcg tgccgatcat gaccagcacc
     1561 gacttgacca gaccggcagc gatgctgagc gcgatgacct cggaactcgc gccgatcgcc
     1621 gcgccggtga ccgggccgac gatgtaggtc acggccccgg cgccgatcgt ggtcatgctg
     1681 acggcgtcgg tgtagccgaa gccatacgcg accgcggccc cgagcacgaa ggacacgacc
     1741 accccgacca gcagcgacag cacgccgctc agcccgcact tcttcagttc gtcgaagctg
     1801 acgccgaacg cggtcgcgac gatggcgaaa tcgcgcagca tcgcgccgcc catcaggccg
     1861 aggccggaga acagcttgag gtcggccagg ccgttcttgc cgccggcggc gacgccgccg
     1921 acgtaggcga gcacgagacc ggcgaggatc gcgatcgccg agccgtggat gcggccgcgg
     1981 gtcaggcggt cggacagcca gtacgacagc cacatggtgg cgccgaccac cgcgaatgcg
     2041 gtcagcaggc cgttcttgcc gagcagggtt tccagggttt cgtacatgat gctcaccgtt
     2101 cgccggaggc gatgaccgtc gtggacaggg aggcgctgtt gccgctcacc gccggcttcg
     2161 gccggcccca gcgatccagc accggaatca gcgccgcgca ggcgatcgtc gcgatgacgc
     2221 cggccagcag cgcggccgga ccgcccttga tcgcggcggc gacgttctgc gtcgaggcca
     2281 tcgcgatgac gatcgggatg tacatcgcgc tccagaactc gatgccgcgc tgcgacatcg
     2341 gtacgagctt caggtcccgg cgcagccggt ccgagatcag gatcagcagc agcatcgcga
     2401 atccgacgcc gccgacgttg gccttgacgc cgatgagctg gccgagcagg tcgccggcga
     2461 acatgccgat cagcgtgcag atcgacagca gggctactcc gtagatgacc atggcaaatc
     2521 tcctcgcttg gtgggcgggt ggggcaggtt gtgcaggttt ggagcgacgg ctctccggcc
     2581 gcctgttctc agggatgtct ccgggcacga cgggcgaaga cgggcacgac ggatcggcgg
     2641 gcgggtgcgg ggcacctcct cg
//
LOCUS       DAOQJH010000426         2654 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_27353_length_2654_cov_3.485187, whole genome shotgun sequence.
ACCESSION   DAOQJH010000426 DAOQJH010000000
VERSION     DAOQJH010000426.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2654)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2654)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2654
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27353_length_2654_cov_3.485187"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..533
                     /locus_tag="PLQ18_09785"
     CDS             <1..533
                     /locus_tag="PLQ18_09785"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HNE01987.1"
                     /translation="VTPGVPPQRRNAAFAQPARSELVRLLHTLKGASGSIGATGLQAE
                     SARLETLLRGGGEPGDIEALSTLLDATLATLARYLDETATPAAAAAVPVPPPASATLS
                     ASLRELQGLLESHRLVPDALVARLRSDCGGHEVAIKLLTAVDNFDYEQASRYLELLIT
                     EYEHDGLGTSGPSRPS"
     gene            493..1476
                     /locus_tag="PLQ18_09790"
     CDS             493..1476
                     /locus_tag="PLQ18_09790"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009110099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNE01988.1"
                     /translation="MTAWGPPDHHDLHEQPPRVLLVDDAPINLQLLASALGRGYELHV
                     ARDGAAALDCIRREPDLDLVLLDIHMPGLDGFEVLTWLREEFPERRLPVIFVTAAGDE
                     QAQTDGFALGAVDYIVRPFSLPVVRARVQTHVALKRQTEHLERLAWLDGLTGAANRRS
                     FDQRLAAEWRRALRNRTPLGLVLFDLDHFKAYNDRHGHPGGDDCLRRIAALGQQSIRR
                     PGDLIARYGGEEFALLLPDTDLAGVHALAERLRRRVETCFACSGGDCNVRGAPGPMTL
                     SAGCASRVPGFNEDPMTLILEADRRLYAAKVAGRNRVVATDDALPAAGGVA"
     gene            1473..>2654
                     /locus_tag="PLQ18_09795"
     CDS             1473..>2654
                     /locus_tag="PLQ18_09795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE01989.1"
                     /translation="MTAQVDVTRHHRPRVLVVDDAPVNRLVLSEALGEDYEVLLADDG
                     PGAVRLAGGEARPDLILLDVNMPGMDGYAVCEQLKESTRTRDIPVIFVTARNEPDDEV
                     RGFTAGAADYITWPLQLPVVQARVRTQLSCSGLIQRLDAQNRVLAERLAALADVGGEV
                     PARLYLRALEHTRDAVMITRADSTIVAVNHAFAALTGYPESEALGRTPRLLRSGRHPP
                     AFYKELYATLAARGRWSGELINRRRDGSLFPAVMTIAAVHDRDGELGHYVSAFSDVTM
                     IRQVQERMDYLTWHDALTGLPNRLLLTDRLRQTLKLGQCDTRVAVAMVLDIERFGALN
                     EALGAQVADAVLGVVASRLRRELCTGDTLARLDGDEFAVVLAGVYDSRESAAHHARLV
                     AG"
BASE COUNT          388 a          951 c          919 g          396 t
ORIGIN      
        1 tggtcacccc gggcgttccc ccgcaacgac ggaacgccgc gttcgcgcag ccggcgcgca
       61 gcgaactcgt gcgcctgctg cacacgctca agggtgccag cggcagtatc ggcgccaccg
      121 gcctgcaggc cgaatcggca cggctggaaa ccctgctgcg cggcggcggc gagcccgggg
      181 acatcgaggc gctgagcacg ctgctcgatg cgacgctggc gacgctggcc cggtatctcg
      241 atgagaccgc gacgccggct gccgctgcgg ccgtgccggt tccgccacct gcctccgcaa
      301 ccctgtcggc ctcgctgcgc gaactgcagg gcttgctcga atcacaccgg ctggtgccgg
      361 atgcgctggt cgcgcgcctg cgcagcgact gcgggggaca cgaagttgca atcaagctgc
      421 tcacggccgt ggataatttt gattatgaac aggcctccag atacctggag ttgctgataa
      481 cggagtacga gcatgacggc ctggggacct ccggaccatc acgaccttca tgagcagccg
      541 ccacgcgtgc tgctcgtcga tgacgcaccg atcaacctcc agttgctggc ctccgcgctc
      601 ggccgcgggt atgagctcca cgtcgcccgc gacggcgcgg cggcgctcga ctgcatccgt
      661 cgcgaacccg atctcgatct ggtcctgctc gacattcaca tgcccgggct cgacggcttc
      721 gaggtgctga cctggctgcg cgaggaattc cccgaacgcc ggctgccggt gatcttcgtc
      781 accgcggccg gagacgaaca ggcgcagacc gacggcttcg cgctcggcgc cgtcgactac
      841 atcgtccgtc cgttctcgct gccggtcgtg cgcgcccgcg tgcagacgca cgtcgcgctg
      901 aaacgccaga ccgaacatct ggagcgtctg gcgtggctcg acggcctgac cggcgccgcc
      961 aaccgtcgca gcttcgacca gcgcctcgcc gccgaatggc gccgtgcgct gcgcaaccgc
     1021 acgccgctcg ggctggtgct gttcgatctc gatcacttca aggcctacaa cgaccgccac
     1081 gggcatccgg gcggcgacga ctgcctgcgc cgcatcgcag ccctcggcca gcagagcatc
     1141 cgccgccccg gcgacctgat cgcgcgctac ggcggcgagg aattcgcgct gctgctgccg
     1201 gacaccgacc tcgccggtgt gcacgcgctg gccgagcgcc tgcgccggcg tgtcgagacc
     1261 tgcttcgcct gcagtggcgg cgactgcaat gtccggggtg cccccgggcc gatgacgctg
     1321 agcgccggct gcgcgagccg ggtgccggga ttcaacgaag atccgatgac gctgatcctt
     1381 gaggccgacc ggcgcctgta cgccgccaag gttgccgggc gcaaccgcgt cgtcgccacc
     1441 gacgacgcgc tgccggcggc aggcggtgtc gcatgacggc gcaggtcgat gtgacccgtc
     1501 atcaccggcc gcgcgtgctg gtcgtcgacg atgcgccggt caaccggctg gtgctgagcg
     1561 aggcgctcgg cgaggactac gaggtgctgc tcgccgacga cggaccgggg gcggtgcgcc
     1621 tcgccggcgg cgaggcgcgc ccggacctga tcctgctcga tgtcaacatg ccgggcatgg
     1681 acggctacgc ggtctgcgag cagctcaagg aatcgacccg cacccgtgac atcccggtga
     1741 tcttcgtcac cgcccgcaac gaacccgacg acgaggtgcg cggtttcacg gccggtgcgg
     1801 ccgactacat cacctggccg ctgcagctgc cggtcgtgca ggcgcgggtg cgtacgcagc
     1861 tgtcctgcag cggcctgatc cagcgcctcg acgcccagaa ccgcgtgctc gccgaacggc
     1921 tggccgcact cgccgatgtt ggcggcgagg tgccggcgcg gctgtatctg cgcgcgctcg
     1981 aacacacgcg cgacgcggtg atgatcacgc gcgccgacag cacgatcgtc gccgtcaacc
     2041 atgcgttcgc ggcgctgacc ggctatccgg agtccgaagc cctgggccgg acgccgcggc
     2101 tgctgcgctc gggccggcat ccgccggcgt tttacaaaga actgtatgcg acgctcgccg
     2161 cgcgcggccg ctggagcggc gagctgatca accgccgccg tgacggctcg ctgttcccgg
     2221 cggtcatgac catcgccgcc gtccacgacc gcgacggcga actcggccac tatgtgtcgg
     2281 cattcagcga tgtgacgatg atccgccagg tgcaggagcg catggattac ctgacctggc
     2341 acgacgcact gaccggcctg ccgaaccgcc tgctgctgac cgaccgcctg cgccagacgc
     2401 tgaagctcgg ccagtgcgac acgcgcgtgg ccgtggcgat ggtgctcgac atcgaacgct
     2461 tcggggcgct gaacgaggcg ctgggcgcgc aggtcgccga tgccgtgctc ggcgtggtcg
     2521 ccagccgcct gcgacgggag ttgtgcaccg gcgacacgct ggcacggctg gacggcgatg
     2581 agtttgcggt cgtgctcgca ggggtgtacg actcgcgcga gagtgccgcc catcacgcgc
     2641 ggctggtcgc cggg
//
LOCUS       DAOQJH010000427         2649 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_27436_length_2649_cov_3.638396, whole genome shotgun sequence.
ACCESSION   DAOQJH010000427 DAOQJH010000000
VERSION     DAOQJH010000427.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2649)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2649)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2649
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27436_length_2649_cov_3.638396"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..395
                     /locus_tag="PLQ18_09800"
     CDS             <1..395
                     /locus_tag="PLQ18_09800"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNE01990.1"
                     /translation="YERIAAASALAAATGLLGLAAEPPYALALGMMMLIGGGMMTTAA
                     ALNSLLQTVVEPRLRGRAMSLYVLAFAGVFPLGALMSGLLAGHFGVQATLALAGLGAL
                     AVVLHYLRRLPRLSARLAGEYRRLDLDG"
     gene            complement(414..770)
                     /locus_tag="PLQ18_09805"
     CDS             complement(414..770)
                     /locus_tag="PLQ18_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012452517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE01991.1"
                     /translation="MFRGILGAALLAAVTFVLPARAGDQDFTLVNRTGVEIHKVYASP
                     HSSDDWEEDILGDDTLPDGESVNITFSPKERAAMWDLRVEDSDGNFITWESLDLLQIS
                     RVTLHYKKGKVWATLD"
     gene            complement(1241..>2649)
                     /locus_tag="PLQ18_09810"
     CDS             complement(1241..>2649)
                     /locus_tag="PLQ18_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cyclic beta 1-2 glucan synthetase"
                     /protein_id="HNE01992.1"
                     /translation="YYQSGGAFGLRDQLQDAMALVHAAPQRLRAQLLLAASRQFIEGD
                     GQHWWHPPGGRGVRTRCSDDYLWLPLATCRYVGATGDTGVLDEVLPFLEGRPVPDGEE
                     SYYDLPSDSQQAASLYEHCRRAIRTGLRYGGHGLPLMLAGDWNDGMNLVGIDGRGESV
                     WLGFFLYAVLQDFAPLAARRGDDEFATLCRREAATLRDNLERHAWDGEWYLRAWFDDG
                     SPLGSQASAECRIDAIAQSWAVLSGAADPARSAQAMDSLERRLVRREAGIIQLFDPPF
                     SGHGPLDPGYIRGYLPGVRENGGQYTHSAVWAVMAFAALGERQRAWELFDLINPLNRT
                     RDAAGIERYRAEPYVMAADVYGVEPHTGRGGWTWYTGSAGWMYRLLLESLLGLTRHAD
                     ALRLDPLLPDGWPGYTLTYRHGASRWHIEVSRGRGGEGAQLSVDGRVRSDGRIPLVDD
                     GAEHRVELKLDGRQRQAA"
BASE COUNT          353 a          966 c          953 g          377 t
ORIGIN      
        1 gctacgaacg catcgccgcc gccagcgcgc tcgcggccgc caccggtctg ctcgggctcg
       61 cagccgaacc gccgtatgcg ctggcgctcg ggatgatgat gctgatcggc ggcggcatga
      121 tgacgacggc cgccgcgctc aacagcctgc tgcagaccgt cgtcgagccg cgcctgcgcg
      181 gccgggcgat gagcctgtac gtgctggcgt ttgccggcgt cttcccgctc ggcgccctga
      241 tgtccggcct gctcgccggg cacttcggcg tgcaggcgac gctggcgctg gccgggctcg
      301 gggcgcttgc ggtggtgctg cactacctgc ggcggctgcc gcggctgagt gcgcggctcg
      361 ccggcgagta ccgccggctc gacctcgacg gctgagccgc ggccagcggg cgctcagtcg
      421 agcgtcgccc acaccttgcc tttcttgtag tgcagcgtga cccgcgagat ctgcagcagg
      481 tcgaggctct cccaggtgat gaagttgccg tcactgtcct cgacgcgcag atcccacatc
      541 gcggcccgct ccttcgggct gaaggtgatg ttgacgcttt cgccatccgg cagcgtgtcg
      601 tccccgagga tgtcctcctc ccagtcgtcc gaactgtgcg gggacgcata gaccttgtgg
      661 atttcgacgc ccgtgcggtt gaccagcgtg aaatcctgat cgcccgcccg ggccggcagc
      721 acgaaagtca cggccgccag cagcgccgcc ccgagaatgc ctctgaacat gattttccga
      781 ctccctttct gcaaacgcat tctgcaaacg cagcccggcc tgccccggcg gcggccggtg
      841 cgggcagtga ctgatggtgg atgtgcaagc gaggcggacc gcggcgctgc cgcgccgccc
      901 tgcgccgggg ggaacgcgcg cgcattctcg aagaatgccc gggacggcca ccggcgcctg
      961 acaatctttc actaccgctg cgcgccggcc ggacggccgc ggtttcatcc gcccgtcggc
     1021 tgcgccccgg cgggcgggcg caccggctcg atgcctgcca cgccgccgac ctgcccggcc
     1081 agccattcga tcagccgccg cacgcgggca gctgcgcccg gggctggaag ccccgggcac
     1141 cagcggcttc gggctgcacg tggccccgtc cgggacatga gctgcgcgcg ggcggccgcg
     1201 gcgggcatat cccggcctgc cggctgtccg tgaccgccgc tcacgccgcc tgccgctgcc
     1261 gcccgtcgag cttcagctcg acgcgatgct cggcgccgtc atcgacgagc gggatgcggc
     1321 cgtcgctgcg cacgcgcccg tcgacgctca gctgcgcacc ctcaccgcca cggccacggc
     1381 tgacctcgat gtgccagcgg ctggcgccgt ggcgataggt gagcgtgtag cccggccagc
     1441 cgtccggcag cagcggatcg agccgcagcg catcggcgtg gcgcgtcagt ccgagcaggg
     1501 attcgagcag cagccggtac atccagccgg ccgagcccgt gtaccaggtc cagccgccgc
     1561 ggccggtgtg cggctcgacg ccatagacat cggccgccat cacgtagggc tcggcgcggt
     1621 agcgttcgat gccggcggcg tcgcgggtgc ggttcagcgg gttgatcagg tcgaacagct
     1681 cccaggcgcg ctggcgctcg ccgagcgcgg cgaaggccat caccgcccac acggcgctgt
     1741 gcgtgtactg gccgccgttc tcgcgcacgc ccggcagata gccgcggatg tagcccggat
     1801 cgagcgggcc gtggccgctg aagggcgggt cgaagagctg gatgatgccg gcctcgcggc
     1861 gcaccagccg gcgttcgagc gagtccatgg cctgcgccga gcgcgccgga tcggctgcgc
     1921 ccgagagcac cgcccagctc tgggcgatgg cgtcgatgcg gcattcggcg ctggcctgcg
     1981 agccgagcgg gctgccatcg tcgaaccagg cgcgcagata ccactcgccg tcccaggcgt
     2041 ggcgttcgag gttgtcacgc agcgtggccg cctcgcgccg gcacagcgtg gcgaactcgt
     2101 catcgccgcg gcgtgccgcc agcggcgcga agtcctgcag caccgcatac aggaagaagc
     2161 cgagccagac gctctcgccg cggccgtcga tgccgacgag gttcatgccg tcgttccagt
     2221 cgccggccag catcagcggc aggccgtgcc cgccgtagcg caggccggtg cggatcgcgc
     2281 gccggcagtg ctcgtagaga ctggcggcct gctgcgaatc gctcggcagg tcgtaatagg
     2341 actcctcgcc gtccggcacc ggccggcctt cgaggaaggg cagcacctcg tcgagcacgc
     2401 cggtatcgcc ggtggcgccg acgtagcggc aggtggcgag cggcagccac aggtaatcgt
     2461 ccgagcagcg cgtgcgcacg ccgcgcccgc ccggcggatg ccaccagtgc tggccgtcgc
     2521 cttcaatgaa ctggcgcgag gcggcgagca ggagctgcgc gcgcaggcgc tggggcgcgg
     2581 cgtgcaccag cgccatcgca tcctgcagct ggtcgcgcag gccgaaggcg ccgcccgact
     2641 ggtagtagc
//
LOCUS       DAOQJH010000428         2640 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_27602_length_2640_cov_5.374855, whole genome shotgun sequence.
ACCESSION   DAOQJH010000428 DAOQJH010000000
VERSION     DAOQJH010000428.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2640
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27602_length_2640_cov_5.374855"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1075
                     /locus_tag="PLQ18_09815"
     CDS             <1..1075
                     /locus_tag="PLQ18_09815"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012982124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HNE01993.1"
                     /translation="GYMDRLDYVSMMCNEHGYVLAIEKLLGVEVPLRAQYIRVMFDEI
                     TRILNHLMWLGAHGLDIGAMTVFLYCFREREDLMDCYEGVSGARMHATYYRPGGVARD
                     LPDSMPKYEYNAYRSKAEIDALNETRGGSMLDFLEAFTERFPGRVDEYETLLTENRIW
                     KQRTVGIGVVTPERALQLGFSGPMLRGSGIEWDLRRKRPYEVYDRLDFDIPVGTHGDC
                     YDRYLVRVEEMRQSNRIIRQCIRWLRDNPGPVMPEGHKVSPPKRTEMKESMEALIHHF
                     KLFSEGYCVPAGEVYAAVEHPKGEFGVYLISDGANKPYRLKVRAPGFAHLSAIDEMVR
                     GHMLADVVAVIGTQDIVFGEIDR"
     gene            1075..1572
                     /gene="nuoE"
                     /locus_tag="PLQ18_09820"
     CDS             1075..1572
                     /gene="nuoE"
                     /locus_tag="PLQ18_09820"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012824814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HNE01994.1"
                     /translation="MSQNKHAVLSEHACHEIDRWVAKYPPERKQSAVLAALREVQHEN
                     GGYLTNELMDAVADYLGLPAIAVYEVASFYSMYELKPVGRHNIAVCTNISCMLRGSDE
                     IVAHIEQKLGIRLGESTADGKFYLKKEEECLAACASAPMMQVDHVYYENLTTAEVDRI
                     LDSLE"
     gene            1580..>2640
                     /gene="nuoF"
                     /locus_tag="PLQ18_09825"
     CDS             1580..>2640
                     /gene="nuoF"
                     /locus_tag="PLQ18_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017925961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HNE01995.1"
                     /translation="MFLNGMLYATKTLENPWSFDTYVQQGGYEAWKKILRGELTSDEV
                     IDIVKASGLRGRGGAGFPTGLKWSFMPRKAPGQKYVVCNSDESEPGTCKDREILIYNP
                     HALVEGMAIAGFAIGATAGYNYMRGEFMDEPYLHFEQAVNEAYAAGLLGRNVQGSGID
                     FDLYPSLGAGAYICGEETALLESLEGKKGQPRFKPPFPANFGLYGRPTTINNTESLAS
                     VPYILRNGAQWFADLGIPGAGGCKIFSVSGHVAKPGNFEIRLGTPFKDLLELAGGVWK
                     GRKLKAVIPGGSSVPVVPAETMLKVNMDYDSISKGAGSMLGSGAVIVMDETTDMVRAL
                     QRLSRFYFSESCGQCTPCR"
BASE COUNT          495 a          798 c          856 g          491 t
ORIGIN      
        1 cggctacatg gaccggctcg actacgtgtc gatgatgtgc aacgagcacg gctacgtgct
       61 ggccatcgaa aagctgctcg gggtcgaagt accgctgcgc gcccagtaca tccgcgtgat
      121 gttcgacgaa atcacgcgca tcctgaacca tctgatgtgg ctcggcgccc acggtctcga
      181 catcggcgcg atgacggtct tcctgtactg cttccgtgag cgtgaagacc tcatggactg
      241 ctacgagggc gtctcgggtg cgcgcatgca tgcgacctat taccggccgg gcggtgttgc
      301 ccgcgatctg ccggacagca tgccgaagta cgaatacaac gcctaccggt cgaaggccga
      361 gatcgatgcg ctgaacgaga cgcgcggcgg ctcgatgctc gatttcctcg aagccttcac
      421 cgagcgtttc ccgggccggg tcgacgaata cgaaaccctg ctgaccgaaa accgcatctg
      481 gaagcagcgc acggtcggca tcggtgtcgt gacgccggag cgcgcgctgc agctcggttt
      541 cagcggcccg atgctgcgcg gttccggcat cgaatgggat ctgcgccgca agcgcccgta
      601 tgaggtctac gatcgcctcg acttcgacat tccggtcggc acgcacggcg attgctacga
      661 ccgctatctg gtgcgcgtcg aggagatgcg ccagtcgaac cggatcatcc gccagtgcat
      721 ccgctggctg cgggacaatc cggggccggt gatgccggaa ggccacaagg tttcgccgcc
      781 caagcgcacc gagatgaagg agtcgatgga agccctcatc catcacttca agctgttctc
      841 cgagggctac tgcgtgccgg ccggcgaagt ctatgcggcc gtcgagcatc cgaagggtga
      901 gttcggcgtg tatctgattt ccgacggtgc caacaagccg taccggctga aggtgcgggc
      961 gcccggtttc gcgcatctgt cggcaatcga cgaaatggtg cgcggtcaca tgcttgccga
     1021 cgtcgtcgcc gtgatcggta cgcaggacat cgtgttcggg gagatcgacc gctgatgagc
     1081 cagaacaagc atgccgtgct gtccgagcac gcctgtcacg agatcgaccg ctgggtcgcc
     1141 aaatatccgc cggagcgcaa gcagtcggcg gtgctggccg cgctgcgcga agtgcagcac
     1201 gaaaacggcg ggtatctcac gaacgaactg atggacgcgg tggccgatta tctcggcctg
     1261 cccgcgatcg cggtctatga ggtcgcgtcg ttctattcga tgtacgaact gaagcccgtc
     1321 ggccggcaca acatcgcggt ctgcacgaac atctcgtgca tgctgcgtgg ctcggacgaa
     1381 atcgtcgcgc acatcgagca gaaactcggc atcagactcg gcgaaagcac tgcggatggc
     1441 aagttctacc tgaagaagga agaggaatgc ctggccgcct gtgcgagtgc accgatgatg
     1501 caggtcgatc acgtctacta cgagaacctg acgaccgccg aggtcgaccg gattctcgac
     1561 agcctggagt gagcgctcga tgttcctgaa cgggatgctg tatgcgacga agaccctgga
     1621 aaatccatgg tcgttcgata cctatgtcca gcagggcggt tacgaggcgt ggaagaagat
     1681 cctgcgcggc gaactgactt cggatgaggt catcgacatc gtcaaggcct cgggcctgcg
     1741 cggccgcggc ggtgccggtt ttccgaccgg tctcaaatgg agtttcatgc cgcgcaaggc
     1801 tccgggtcag aagtatgtcg tctgcaattc ggacgagtcc gaacccggta cctgcaagga
     1861 ccgtgaaatc ctgatctata acccgcatgc gctggttgag ggcatggcga tcgccggttt
     1921 cgcgatcggg gccaccgccg gttacaacta catgcgcggc gagttcatgg acgagcccta
     1981 cctgcatttc gagcaggcgg tcaacgaggc ctacgccgcc ggtctgctcg gcaggaacgt
     2041 ccagggttcg ggcatcgatt tcgacctgta tccgtcgctc ggtgccggcg cctacatctg
     2101 cggcgaggaa accgcgctgc tcgaatcgct ggaaggcaag aagggccagc cgcgcttcaa
     2161 gccgccgttc ccggcgaatt tcggtctgta cggccgtccg acgacgatca acaacaccga
     2221 gtcgctggcc tcggtgccgt acatcctgcg caacggtgcc cagtggtttg ccgatctcgg
     2281 cattccgggg gccggtggct gcaagatctt ctcggtgtcg ggacacgtcg ccaagcccgg
     2341 caatttcgag atccgtctcg gcacgccgtt caaggatctg ctggaactgg caggcggggt
     2401 ctggaagggg cgcaagctca aggccgtgat ccccggcggt tcgtcggtgc cggtggtgcc
     2461 ggccgagacc atgctcaagg tcaacatgga ctatgactcg atctcgaagg gtgccggttc
     2521 gatgctcggc tccggcgcgg tcatcgtcat ggacgagacc accgacatgg tcagggcgct
     2581 gcagcgtctg tcgcgattct atttttccga gtcctgcggc cagtgcacgc cgtgtcgcga
//
LOCUS       DAOQJH010000429         2640 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_27608_length_2640_cov_3.496712, whole genome shotgun sequence.
ACCESSION   DAOQJH010000429 DAOQJH010000000
VERSION     DAOQJH010000429.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2640
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27608_length_2640_cov_3.496712"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..646
                     /locus_tag="PLQ18_09830"
     CDS             <1..646
                     /locus_tag="PLQ18_09830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE01996.1"
                     /translation="AAAVASRLVATEAALRQAIEAAVASAAREAVQSMAPALVQDLVY
                     AAVPEAVRSEVGDRLPLVQSAARDAALAVAEDAAGASAGRVRDLAASIVEERIAQGRN
                     EWLHSLDASIESGPLAARIGQMARDAAQQGARSAAESLFAQVREGLMSELRGELGTLR
                     GRVGDAPTREQLDEALAAARKAEAHAEATARQMRIGMVLLGLGIVASVAIGVLL"
     gene            855..>2640
                     /locus_tag="PLQ18_09835"
     CDS             855..>2640
                     /locus_tag="PLQ18_09835"
                     /inference="COORDINATES: protein motif:HMM:NF015859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNE01997.1"
                     /translation="MARAQRKANIEATSAGNADERRSWLPALATLIGGLLLTAAIFLL
                     LASGERERAHDEFERAARDRFLSVARELEQGLTGLQGLAAFVYTNPGMSAEAFDALSR
                     HLLGLSPGLDTLAWAARDTQGGGDRYLLSRLYPAEGASLVAGADLLARPEFAATVLAA
                     RDGGRLALSEPFPRGGDGESRGVLAAVPVYRTLISGADERRRRQDIVGVAIAVLDPQR
                     LVEGALSRLDPVKVELRLFDLGAPRGRQLLYHPLGSLRGAAMASYLADPEAFGAVEHL
                     QRRIEIAQREWLVVATPAPGQFFASLGSRPWWALGVGLALTLAVAFWLRATVGRHGQV
                     EAMVALREAELSESALRFHQLTEGAPLVLWISSIENGQMLYLSPAFESIWGFTPEQVF
                     QRPGLRRELVHPEDRDRLRRLVRSAAQNEGYDTEYRIQRQDGELRWIRERAHAVRNAA
                     GVVYRLAGLSEDVTAQHRHAAEAAVRDARLRASFESLPEAVLIVGSSGCIESMNPAAE
                     RQFGYAADELLGGPVAVLLPGYDPLAPLDPSRPLSGVRRGGGRFPALVPSSDYDDDNG
                     RHHVCLVHDLTALGQSQTVSSRERERASS"
BASE COUNT          317 a          944 c         1016 g          363 t
ORIGIN      
        1 ggcggcggcg gtcgcctcgc ggctggtggc gaccgaggct gcgctgcgtc aggccatcga
       61 ggccgcggtc gcaagcgccg cgcgcgaggc cgtgcagtcg atggcgccgg cgctggtgca
      121 ggatctcgtc tatgccgccg tgcccgaggc cgtgcgcagc gaggtcggcg accggctgcc
      181 gctggtgcag tcggcggcgc gcgacgcggc gctggcggtg gccgaggatg ccgccggggc
      241 cagtgccgga cgggtgcgcg atctcgccgc cagcatcgtc gaggagcgca tcgcgcaggg
      301 ccgcaacgaa tggctgcaca gcctcgatgc ctcgatcgaa tccggaccgc tcgctgcgcg
      361 catcggccag atggcgcgcg acgccgccca gcagggcgcg cgctcggccg ccgaatcgct
      421 gttcgcgcag gtgcgggaag ggctgatgag cgagctgcgc ggcgagctcg gcacgctgcg
      481 cggccgcgtc ggcgatgccc cgacccgcga acagctcgac gaggcgctcg ccgccgcgcg
      541 caaggccgag gcgcacgccg aggcgacggc ccggcagatg cgcatcggca tggtcctgct
      601 cggcctcggc atcgtcgcct cggtggccat cggcgtgctg ctctgaggct gcagcgctga
      661 tccgaccccg accccgcgcc cggcgcgggg ttttcgttgc tggcggcggc cggcgcgccg
      721 gcggcggacg ataatcgcgc ccggtgccgg cgccggaacg ggcagcagcg cctgccggcg
      781 gccgggaagg atgttctgga gtccgtctca atgcacggcc gtgcggccgg tgcagccagc
      841 gaggatcacg aggcatggcg cgtgcgcagc ggaaagcaaa catcgaggcc acgagcgcag
      901 ggaacgccga cgagcggcga tcgtggctgc cggcgctcgc gacgctgatc ggcggactgc
      961 tgctgaccgc ggcgatcttc ctgctgcttg cgagcggcga gcgcgagcgt gcccacgatg
     1021 agttcgagcg cgcggcgcgc gaccgcttcc tgtcggtggc gcgcgagctg gagcagggcc
     1081 tgaccggcct gcaggggctc gcggccttcg tctacaccaa ccccggcatg tctgccgagg
     1141 ccttcgatgc gctgtcgcgc cacctgctgg gtttgtcccc ggggctggac acgctggcct
     1201 gggcggcgcg cgacacgcag ggcggcggtg atcgctacct gctgtcgcgg ctgtacccgg
     1261 ccgaaggcgc gagcctggtc gccggtgccg atctgctcgc ccggcccgag ttcgccgcca
     1321 ccgtgctcgc ggcgcgcgac ggcggccggc tggcgctgtc cgagcccttc ccgcgcggcg
     1381 gcgacggcga gtcgcgcggc gtgctggcgg cggtgccggt gtaccgcacg ctgatcagcg
     1441 gcgccgacga acgccgccgc cgtcaggaca tcgtcggcgt cgcgatcgcc gtgctcgatc
     1501 cgcagcgtct ggtcgagggc gcgctgtcgc ggctcgatcc ggtcaaggtc gagctgcgcc
     1561 tgttcgatct cggcgcaccg cgcggccgcc agctgctgta tcacccgctc ggcagcctgc
     1621 gcggtgcggc gatggcgagc tatctcgccg atcccgaagc cttcggcgcg gtcgagcacc
     1681 tgcagcgcag gatcgagatc gcccagcgcg aatggctggt ggtggccacg ccggcgccgg
     1741 ggcagttctt cgcctcgctc ggcagccggc cgtggtgggc gctcggcgtc ggactggcgc
     1801 tgacgctggc ggtggcgttc tggctgcggg cgacggtcgg ccgtcacggc caggtcgagg
     1861 cgatggtcgc gctgcgcgag gccgagctgt ccgagagcgc gctgcgcttc catcagctca
     1921 ccgaaggcgc gccgctggtg ctgtggatct ccagcatcga gaacggccag atgctgtacc
     1981 tgagcccggc cttcgaatcg atctggggct tcacgcccga gcaggtgttc cagcgcccgg
     2041 gcctgcgccg cgaactcgtg catccggagg atcgcgaccg gctgcgccgg ctggtgcgca
     2101 gcgccgcgca gaacgagggc tacgacaccg agtaccgcat ccagcgccag gacggcgagc
     2161 tgcgctggat ccgcgagcgc gcccacgccg tgcgcaacgc cgccggcgtc gtctaccggc
     2221 tcgccggcct gtccgaggac gtgaccgcgc agcaccggca cgcggccgag gccgcggtgc
     2281 gggatgcgcg cctgcgcgcc tccttcgagt cgctgcccga ggccgtgctg atcgtcggtt
     2341 ccagcggctg catcgagtcg atgaatccgg cggccgagcg ccagttcggt tatgcggccg
     2401 acgaactgct cggcggaccg gtcgcggtgc tgctgccggg ctacgatccg ctggcgccgc
     2461 tcgatccgtc ccggccgctg agcggcgtgc gccgcggcgg cgggcgcttc ccggcgctgg
     2521 tgccgagcag cgattacgac gacgacaacg gtcgccatca cgtctgtctg gtccacgacc
     2581 tgaccgcgct cggccagagc cagaccgtca gctcgcgcga gcgcgagcgc gcgtcgtcga
//
LOCUS       DAOQJH010000430         2637 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_27664_length_2637_cov_3.483346, whole genome shotgun sequence.
ACCESSION   DAOQJH010000430 DAOQJH010000000
VERSION     DAOQJH010000430.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2637)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2637)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2637
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27664_length_2637_cov_3.483346"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..533
                     /locus_tag="PLQ18_09840"
     CDS             <1..533
                     /locus_tag="PLQ18_09840"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE01998.1"
                     /translation="ALPLRARPLLRALAASIASLAPAAATDPPPPAVTLLTQPKALRA
                     RVLLAEDNPINEEVACIILGELGCEVHAVTDGRAAVEAWRDGHWDMIFMDGQMPVLDG
                     IGATAEIRRLEQELDRAHTPIIALTAHAVHGDRERYLQAGMDDYVSKPFTPEDLERAL
                     AQWLGPADAQAVLRAG"
     gene            complement(629..1819)
                     /locus_tag="PLQ18_09845"
     CDS             complement(629..1819)
                     /locus_tag="PLQ18_09845"
                     /inference="COORDINATES: protein motif:HMM:NF018805.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin-like domain-containing protein"
                     /protein_id="HNE01999.1"
                     /translation="MRPLSRLPRWRPFTVLVAVLAGTGLWLWHSGHPPPPAPPPATAV
                     SAALGGGSDGAWARAYAPRPFTFPADHGAHADFRNEWWYVTGHLRAADGRRFGYQFTL
                     FRIGLHPGPLPADSAWRAQQWYLGHFAVSDLDDGRHQARERYSRAALGLAGATREPLA
                     IWLEDWRLDGGPDAGFPMRLRARDGGLSLDLQLTPLAPVVLQGEDGLSRKSSEPGNAS
                     YYYSFPRLASRGTLSLDGRNPALAVEGTSWLDREWSTSALGPGQSGWDWFALQLADGR
                     NLMLYRLRRSDGTTDPHSAGTLSDATGRTTRLSASGFTLTPEGRWTSPDGAAHYPAGW
                     QLDIPSLGLKLRVRPQLADQEMRLSVRYWEGAVAVDGHDAQGPVHGEGYLEMTRYEDG
                     PAAP"
     gene            complement(1847..>2637)
                     /locus_tag="PLQ18_09850"
     CDS             complement(1847..>2637)
                     /locus_tag="PLQ18_09850"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNE02000.1"
                     /translation="RLLHGDPARAWAAFDAGSVLIGEPLATRLRLGPGDELALATDHG
                     PRRYPIAGVYTDYGSEHGRALIHPATAARDFAPGAPASAGVFLKPGVDPQAARAGLEA
                     RLAPLQAVELRPNHTIVERSLAIFDRTFTITAVLRVLALVVAAAGVLGTLLALALERG
                     REFAVLRALGLLPGELLRLLAIECGLLGLAAGLLALPLGGLTGLILTDIVNRRAFGWS
                     LPLTLPLDALVQTVLLAVIAALAAGLYPAWRLARTPPAAALREE"
BASE COUNT          360 a          924 c         1028 g          325 t
ORIGIN      
        1 tggcgctgcc gctgcgcgcc cggccgctgc tgcgcgcgct ggccgcgagc attgccagcc
       61 tcgcccctgc ggccgccacc gatccgcccc cgccggcggt cacgctgctg acgcagccga
      121 aggcattgcg ggcgcgcgtg ctgctcgccg aggacaaccc gatcaacgag gaggtcgcgt
      181 gcatcatcct cggcgaactc ggctgcgagg tgcacgccgt caccgacggc cgcgccgcgg
      241 tcgaggcctg gcgcgacggc cactgggaca tgatcttcat ggatggccag atgccggtgc
      301 tcgacggcat cggcgccacc gccgaaatcc gccggctgga gcaggagctc gaccgcgcgc
      361 acacgccgat catcgcgctg accgcgcacg ccgtgcatgg tgatcgcgaa cgctatctgc
      421 aggccggcat ggacgactac gtcagcaagc cgttcacgcc cgaggacctc gaacgcgcgc
      481 tggcgcagtg gctggggccg gccgatgcgc aggcggtgct gcgggcgggc tgagcggggg
      541 ctgcggttcg cgtggccgaa aagcccggat cgccgtgccc gcgtgcggca tgcccgatcc
      601 cgtcgagcat cccgccgatg ccccggcctc agggcgcggc cggcccgtcc tcgtagcgcg
      661 tcatctccag atagccctcg ccgtgcacgg ggccttgcgc atcgtggccg tcgacggcga
      721 ccgcgccttc ccagtagcgc accgacagcc gcatctcctg atcggcaagc tgcgggcgca
      781 cgcgcagctt cagcccgagc gacgggatgt ccagctgcca gccggccggg tagtgcgcgg
      841 caccgtcggg gctggtccag cggccttcgg gggtgagggt gaagccggag gcggagaggc
      901 gcgtcgtgcg gccggtggcg tcactgagcg tgccggccga gtgcgggtcg gtcgtgccgt
      961 cgctgcggcg caggcggtac agcatcaggt tgcggccgtc ggcgagctgc agcgcgaacc
     1021 agtcccagcc gctctggccg gggccgagcg cgctggtgct ccattcgcga tcgagccagc
     1081 tcgtgccctc gaccgcgagc gcggggttcc ggccatcgag gctcagcgtg ccgcggctcg
     1141 cgaggcgcgg gaacgagtag tagtacgagg cgttgccggg ctcgctgctc ttgcggctca
     1201 ggccgtcctc accctgcagc accacgggtg cgagcggcgt gagctgcaga tcgagcgaca
     1261 ggccgccgtc acgggcacgc aggcgcatcg ggaagccggc atcgggaccg ccgtcgaggc
     1321 gccagtcctc gagccagatc gccagcggtt cgcgggtggc accggcgagg ccgagcgcgg
     1381 cgcggctgta gcgttcgcgg gcctgatgcc ggccgtcgtc gagatccgag accgcgaagt
     1441 ggccgaggta ccactgctgc gcgcgccagg ccgagtcggc cggcagcggg ccggggtgga
     1501 ggccgatgcg gaacagcgtg aactggtagc cgaagcggcg gccatcggcg gcgcgcaggt
     1561 gaccggtcac gtaccaccat tcgttgcgga agtccgcgtg cgcgccgtga tcggccggaa
     1621 acgtgaaggg gcgcggcgcg tatgcgcgtg cccaggcgcc gtcgctgccg ccgccgaggg
     1681 cagccgagac ggcggtggcc ggtggcgggg ccggcggtgg cgggtggccg ctgtgccaga
     1741 gccagagccc cgtgccggcg agcacggcga cgaggacggt gaacggacgc cagcgcggca
     1801 gccggctgag cgggcgcatc gctttcattc ctcgttcatt cctctttcat tcctcgcgca
     1861 gcgctgcggc cggcggcgtg cgtgccagcc gccaggccgg gtacaggccg gcggcgagcg
     1921 cggcgatgac cgcgagcagc acggtctgca cgagcgcatc gagcggcagc gtcagcggca
     1981 gcgaccagcc gaaggcgcgg cggttgacga tgtcggtcag gatcaggccg gtcagtccgc
     2041 cgagcggcag cgccagcagc ccggcggcca gcccgagcag gccgcattcg atggcgagca
     2101 ggcgcagcag ctcgcccggc agcaggccga gcgcgcgcag caccgcgaat tcgcgcccgc
     2161 gttcgagcgc gagcgcgagc agcgtgccga gcacgccggc ggcggcaacc accagcgcca
     2221 gcacgcgcag cacggcggtg atcgtgaagg tgcgatcgaa gatcgccagc gagcgctcga
     2281 cgatcgtgtg gttcgggcgc agctcgaccg cctgcagcgg cgcgaggcgt gcttcgagtc
     2341 cggcgcgcgc cgcctgcgga tcaacgcccg gtttcaggaa cacgccggcc gaggccggtg
     2401 cgcccggtgc gaagtcgcgc gcggcggtgg ccgggtggat cagcgcgcgg ccgtgttcgg
     2461 agccgtagtc ggtgtagacg ccggcgatcg ggtagcggcg cgggccgtgg tcggtcgcaa
     2521 gcgcgagttc atcccccgga ccgagccgca ggcgcgtcgc cagcggttcg ccgatcagca
     2581 cgctgccggc atcgaaggcc gcccaggcgc gggccgggtc gccgtgcagc agcctgt
//
LOCUS       DAOQJH010000431         2636 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_27681_length_2636_cov_4.801240, whole genome shotgun sequence.
ACCESSION   DAOQJH010000431 DAOQJH010000000
VERSION     DAOQJH010000431.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2636
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27681_length_2636_cov_4.801240"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="PLQ18_09855"
     CDS             <1..322
                     /locus_tag="PLQ18_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003632409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HNE02001.1"
                     /translation="IVLTMGMAVDANVLIYERLREELRLGLTPQAAIKAGFERAFATI
                     LDANVTHLIAGIALFSLGSGPVKGFAVTLIIGILTSMFTAVTVSRALVNLVYGGRKVG
                     RISV"
     gene            336..1268
                     /gene="secF"
                     /locus_tag="PLQ18_09860"
     CDS             336..1268
                     /gene="secF"
                     /locus_tag="PLQ18_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008931692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HNE02002.1"
                     /translation="MQLIPHTLDIRFMRFGRMSLILTLLLMLVCAGALVFKGLNYGLD
                     FTGGTVIEVRYAQAIEMPQVRSALAAGGFADAQTQYIGSAAEVLIRVPPHEGSDAAAL
                     STHILDALKLGNDGAVEVRRVEFVGPQVGGELVQNGSLAAVFAMLGILVYVAFRFEWR
                     LALGAIAATLHDVVFCIGVYALFGIEFDLTGLAAILAVIGYSVNDTVVVFDRIRENFR
                     RLRKDTPERVIDISINETLARTIMTSMTTLLAVVVLYVWGGDAIHTFSLIMIVGVLIG
                     TYSSIYVASATALHLGLSREDLLAKVKEEGDARP"
     gene            complement(1424..1768)
                     /locus_tag="PLQ18_09865"
     CDS             complement(1424..1768)
                     /locus_tag="PLQ18_09865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02003.1"
                     /translation="MQKKTLAFLAPPFAIARFGGRVAWCAAPIGTVWMSAIVGILIGI
                     QGDMSTATREPMLWVIALGLAIWAVSSVWALMVIGGVDHDVAHDADSTLEHTVHAEED
                     ELDPLEQIRGMR"
     gene            complement(2031..>2636)
                     /locus_tag="PLQ18_09870"
     CDS             complement(2031..>2636)
                     /locus_tag="PLQ18_09870"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011587798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HNE02004.1"
                     /translation="LAEESGAAGESDYQWIIDPLDGTTNFLHGHPQFAISIALKVRGR
                     LESALIHDPLRQETYAAARGEGATLNDRRIRVKPAAGLDGALLGTGFPFRAPRHMPAY
                     LACFAALAEHCGEMRRAGSAALDLAYVAAGRLDGFWEIGLAEWDMAAGVLLVQEAGGL
                     VGDFGGGAEYFRTGNVVAGTPKVFKGLLQTIQPHLTPALSR"
BASE COUNT          392 a          878 c          922 g          444 t
ORIGIN      
        1 catcgtgctg acgatgggca tggcggttga cgccaacgtg ctgatctacg agcgcctgcg
       61 cgaggagctg cgcctgggcc tgacgccgca ggcggcgatc aaggccggct tcgagcgggc
      121 gttcgcgacc atcctcgacg ccaacgtcac gcacctgatc gccggcatcg cgctgttctc
      181 gctcggctcc ggcccggtca agggcttcgc ggtgacgctg atcatcggca tcctgacctc
      241 gatgttcacg gccgtcaccg tcagccgcgc actggtcaac ctcgtctacg gcggtcgcaa
      301 ggtcggccgc atctcggtgt aacggagcgc gtgccatgca gctgattccc cacacgctcg
      361 acatccgctt catgcgcttc gggcgcatgt cgctgatcct gaccctgctg ctgatgctgg
      421 tctgcgccgg cgcgctggtg ttcaagggcc tcaactacgg gctcgacttc accggcggca
      481 ccgtcatcga ggtgcgctac gcgcaggcca tcgagatgcc gcaggtgcgc tcggcgctcg
      541 ccgccggcgg cttcgccgat gcgcagaccc agtacatcgg ctcggccgcc gaggtgctga
      601 tccgcgtgcc gccgcacgag ggcagcgacg ccgcggcgct cagcacgcac atcctcgatg
      661 cgctgaagct cggcaacgac ggtgcggtcg aggtgcgccg cgtcgagttc gtcggcccgc
      721 aggtcggcgg cgagctggtg cagaacggtt cgctggcggc ggtgttcgcg atgctcggca
      781 tcctcgtcta cgtcgccttc cgcttcgaat ggcggctcgc gctcggcgcg atcgctgcga
      841 ccctgcacga cgtggtgttc tgcatcggcg tctacgcgct gttcggcatc gagttcgacc
      901 tgaccggcct cgccgcgatc ctcgccgtga tcggctactc ggtgaacgac accgtcgtcg
      961 tgttcgaccg catccgcgag aacttccgcc ggctgcgcaa ggacacgccg gagcgcgtca
     1021 tcgacatctc gatcaacgag acgctggccc gcacgatcat gaccagcatg acgacgctgc
     1081 tcgcggtggt cgtgctctac gtctggggcg gcgacgcgat ccacaccttc tcgctgatca
     1141 tgatcgtcgg cgtgctgatc ggaacgtact cgtcgatcta cgtcgcctcg gcgactgcac
     1201 tgcacctcgg cctgtcacgc gaggatctgc tggcgaaggt gaaggaggag ggcgacgcgc
     1261 ggccgtgagg ccgggcacac cgccatcatc aggaatgctt cgcttttcag cccctcggcc
     1321 ctcgcgggag tgagatgggg agagaagggg gcatgaaaaa ggggaggccg gccggcctcc
     1381 cctttgtgtt ttccggccgc cttgcgacgg ccgcgcggcg cgttcagcgc atgccgcgga
     1441 tctgttcgag cgggtcgagt tcgtcttcct cggcgtgcac cgtgtgttcg agcgtgctgt
     1501 cggcatcgtg cgcgacatcg tgatcgacgc cgccgatgac catcagcgcc cacactgagg
     1561 acacggccca gatcgccagg ccgagggcga tgacccacag catcggttcg cgggtggcgg
     1621 tgctcatgtc gccctggatg ccgatcagga tgccgacgat cgcgctcatc cagaccgtgc
     1681 cgatcggagc tgcgcaccag gcgacgcggc cgccgaagcg ggcgatggca aacggcggcg
     1741 cgagaaaggc gagggttttc ttctgcatgc tgacctcctg aatccccgtg atcgggtatg
     1801 ccgcgcttgc ttatgggggc gggaactgtg gcggtacgtc ggagtgcgcg gtcagtcccg
     1861 aagttcgctg ccgtggcaga cagccttcgg ccgggtgcaa accggggtca gactagagca
     1921 gtccgatgcg gccgtgcagg ctcttgcgca tgacggcaac gaaagtcgtt gccatcatgg
     1981 catccggtaa tttatggcgc gccgcagcag agatccggag gcggggggat tcagcggctc
     2041 agtgccggcg tcaggtgcgg ctggatggtc tgcagcaggc ccttgaatac cttgggcgtg
     2101 ccggcgacga cgttgccggt gcggaagtac tcggcgccgc cgccgaagtc accgaccagg
     2161 ccgccggctt cctgcaccag cagcacgccg gcggccatgt cccattcggc cagaccgatc
     2221 tcccagaagc cgtcgaggcg gccggcggcg acgtaggcga gatcgagcgc cgccgagccg
     2281 gcgcggcgca tctcgccgca gtgctcggcc agcgccgcga agcaggccag atacgccggc
     2341 atgtgccgcg gcgcccggaa cgggaagccg gtgccgagca gcgcgccgtc gaggccggcc
     2401 gcgggcttga cgcggatgcg gcggtcattg agcgtcgcgc cctcgccgcg cgcggcggcg
     2461 taggtttcct gacgcagcgg atcgtggatc agcgcggatt cgaggcgccc gcgcaccttc
     2521 agcgcgatcg agatcgcgaa ctgcgggtgg ccgtgcagga agttggtcgt gccgtcgagc
     2581 ggatcgatga tccactggta atcggattcg ccggccgcgc cgctctcctc ggcgag
//
LOCUS       DAOQJH010000432         2629 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_27804_length_2629_cov_4.034188, whole genome shotgun sequence.
ACCESSION   DAOQJH010000432 DAOQJH010000000
VERSION     DAOQJH010000432.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2629)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2629)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2629
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27804_length_2629_cov_4.034188"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(8..1915)
                     /locus_tag="PLQ18_09875"
     CDS             complement(8..1915)
                     /locus_tag="PLQ18_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HNE02005.1"
                     /translation="MPNDAALAVADAPHTPKKNLTTLTLAALGVVYGDIGTSPLYALR
                     ECFSGPHGIIATEANVLGVISLIIWSLLLLVSVKYVAFVLRADFRGEGGILALMALAC
                     SRRFATRRRHWSVLILGVFGATLFYGDAMITPAISVLSAIEGLNVATTVFEPWVVPLT
                     LLVLIGLFLVQSHGTDRVGKFFGPVMLVWFGALAVFGVAEMLKRPDILAAVHPRHAYF
                     FFRDNQWHAFVTLGAVFLVMTGGEALYADMGHFGRRPIRRAWFALVLPAILLNYFGQG
                     ALLLANPAAAENPFYLLVPAPLLYPMVGLATAATVIASQAVISGVFSLTRQAMQLGYA
                     PRMSIEHTSAAEIGQIYIPAVNWTLLVAVLLLVLGFESSSRLAAAYGIAVTLTMVITT
                     LLLAVVTRRVWGWHPALVAVCILPFLAADLAFLGANALKIPQGGWFPLVVSALLFTFM
                     TTWKRGRKLLARRLREEAIPIKLFVDDAADGMIQRVSGTAIFLTSNPDGVPHTLLHNL
                     KHNKVMHERVVLLTVRTEEVPFVREDKRVEVETIGSGFYRVHMRFGFTEDPDVQAALA
                     LCGPHGLEFELMETTFFLGRETLISTSRPGMARWRERLFIAMSRSSARAMDFFNIPIN
                     RVVELGTQIEL"
     gene            complement(2024..2362)
                     /locus_tag="PLQ18_09880"
     CDS             complement(2024..2362)
                     /locus_tag="PLQ18_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF190 domain-containing protein"
                     /protein_id="HNE02006.1"
                     /translation="MNPSESGIALRFHLSEGRRHHGRLLWEWLLELARELDLPGGTAY
                     RALAGYGRHRRLHEAHFFELAGDVPIDVIFILRPADEARLLERIDAEGLALFYARMPI
                     EFGLTGPAGD"
     gene            complement(2396..>2629)
                     /locus_tag="PLQ18_09885"
     CDS             complement(2396..>2629)
                     /locus_tag="PLQ18_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015724429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CrcB family protein"
                     /protein_id="HNE02007.1"
                     /translation="LGVFQALPALAPEWRLALATGFLGGLTTFSTFSAEIVTLLARGE
                     LVWGGAAILMHVGGSLAATAVGLALVGWLRTLG"
BASE COUNT          461 a          892 c          935 g          341 t
ORIGIN      
        1 gccgccctca cagctcgatc tgcgtgccca gctccacgac gcggttgatc gggatgttga
       61 agaagtccat cgcccgcgcc gagcttcgcg acatcgcgat gaacagccgc tcgcgccagc
      121 gcgccatgcc ggggcgcgag gtcgagatca gggtttcgcg gccgaggaag aaggtcgtct
      181 ccatcagctc gaactccaga ccgtgcggcc cgcacagcgc cagcgccgcc tgcacgtccg
      241 ggtcctcggt gaagccgaag cgcatgtgca cgcggtagaa gccggagccg atggtctcga
      301 cctcgacgcg cttgtcctcg cgcacgaacg gcacttcctc ggtgcgcacg gtcagcagca
      361 cgacgcgctc gtgcatgacc ttgttgtgct tgaggttgtg cagcagcgtg tgcggcacgc
      421 cgtccgggtt cgaggtcagg aagatcgccg tgccggaaac gcgctggatc atgccgtcgg
      481 cggcatcgtc gacgaacagc ttgatcggga tcgcctcctc gcgcaggcgc cgggcgagca
      541 gcttgcggcc gcgcttccag gtggtcatga acgtgaacag cagcgcgctg accaccagcg
      601 ggaaccagcc gccctgcgga atcttcagcg cgttggcgcc gaggaaggcc agatccgcgg
      661 cgagaaacgg caggatgcac accgccacca gcgccggatg ccagccccag acccggcgcg
      721 tgaccacggc gagcagcagg gtcgtgatca ccatcgtcag cgtcacggcg atgccgtagg
      781 ccgctgccag ccggctcgat gactcgaagc cgagcacgag cagcagcacc gccaccagca
      841 gcgtccagtt caccgccggg atgtagatct ggccgatctc ggcggccgag gtgtgctcga
      901 tgctcatgcg cggcgcgtag ccgagctgca tcgcctggcg cgtcagcgag aacacgccgg
      961 aaatcaccgc ctgcgaggcg atcacggtcg ccgcggtcgc gagcccgacc atcgggtaca
     1021 gcagcggcgc cggcaccagc aggtagaacg gattctcggc cgccgcgggg ttggcaagca
     1081 gcagcgcgcc ctgaccgaag tagttgagca ggatcgccgg cagcaccagc gcgaaccagg
     1141 cgcggcggat cggccggcgc ccgaagtggc ccatgtcggc gtacagcgcc tcgccgccgg
     1201 tcatgaccag gaacacggca ccgagcgtga cgaaggcatg ccactggttg tcgcggaaga
     1261 agaagtacgc gtgccgcgga tgcacggcgg cgaggatgtc ggggcgcttg agcatctcgg
     1321 cgacgccgaa caccgccagc gccccgaacc agaccagcat caccggcccg aagaacttgc
     1381 cgacccggtc ggtgccgtgc gactgcacga ggaacaggcc gatcagcacc agcagcgtca
     1441 gcggcacgac ccagggctcg aacaccgtgg tcgcgacgtt gaggccctcg atcgccgaca
     1501 gcaccgagat cgccggcgtg atcatcgcgt cgccgtagaa cagcgtcgcg ccgaacacgc
     1561 cgaggatcag cacggaccag tgccggcggc gggtcgcgaa gcgccggctg caggcgagcg
     1621 ccatcagcgc caggatgccg ccctcgccgc ggaagtcggc gcgcagcacg aaggcgacgt
     1681 acttgaccga gaccagcagc agcagcgacc agatgatcag cgagatcacg ccgagcacgt
     1741 tggcctcggt cgcgatgatg ccgtgcgggc cgctgaagca ttcgcgcagc gcgtacagcg
     1801 gactggtgcc gatgtcgccg tagacgacgc cgagggcggc cagcgtcagc gtcgtcaggt
     1861 tcttcttcgg ggtgtgcggg gcgtcagcga cggccagtgc ggcatcgttc ggcatgcggg
     1921 cggtttccgg aagctcgggg tcggagggct tgcacagcca gagcggtccg caccgggcgc
     1981 ggtgcaggcc ggaccgtcag gaatgctgcg actgcaatat aggtcagtcc ccggcggggc
     2041 cggtgaggcc gaattcgatc ggcatgcggg cgtagaagag cgcgaggccc tcggcatcga
     2101 tgcgttcgag cagccgtgcc tcgtcggccg gacgcaggat gaagatcacg tcgatcggca
     2161 catcgccggc cagctcgaag aaatgcgcct cgtgcaggcg gcggtggcgg ccgtagccgg
     2221 cgagcgcccg gtaggcggtg ccgccgggca ggtcgagctc gcgcgccagc tccagcagcc
     2281 attcccacag cagccggccg tggtggcgtc ggccttcgga caggtggaag cgcagcgcga
     2341 tgccggattc gctcgggttc atggcttcat cgtctccccg tctttgcggt cttgctcagc
     2401 ccagggtccg cagccagccg accagcgcca gcccgacggc ggtcgcggcc agcgagccgc
     2461 cgacgtgcat caggatcgcc gcgccgcccc agaccagttc gccgcgtgcg agcagcgtga
     2521 cgatctcggc cgagaaggtc gagaaggtcg tcaggccgcc gaggaagccg gtcgcgagcg
     2581 ccagccgcca ttccggggcc agcgccggca gcgcctggaa cacgccgag
//
LOCUS       DAOQJH010000433         2625 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_27874_length_2625_cov_4.128405, whole genome shotgun sequence.
ACCESSION   DAOQJH010000433 DAOQJH010000000
VERSION     DAOQJH010000433.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2625)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2625)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2625
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27874_length_2625_cov_4.128405"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1042
                     /gene="proS"
                     /locus_tag="PLQ18_09890"
     CDS             <1..1042
                     /gene="proS"
                     /locus_tag="PLQ18_09890"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015280830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HNE02008.1"
                     /translation="DASGYAANVELAEALAPAGERPAAGEALTRVHTPKVKTIAELAA
                     FLKIPAERTLKAVVVDGSDGRPVLLLLRGDHELNEIKAGKLPEVAEPLTFSTPAAISA
                     AFGAQPGSLGPVGFDGVVIADRSAAVLADAVTGANEDDWHLSGFNFGRDCPEPRVADL
                     RNVVAGDPSPDGQGRLSIRRGIEVGHIFQLGRKYSDALAANVLGEDGQNLTVTMGCYG
                     IGVSRVVAAAIEQNWDERGILWPAAMAPFQLALLPMNAHKSAAVREAVEQLYADCRAA
                     GIDVLLDDREARPGVMFADMELIGIPHRVVIGEKGLAAGSVEYKGRRDAEALAVPLAG
                     LIDFLRERLGAA"
     gene            1211..1654
                     /gene="nudB"
                     /locus_tag="PLQ18_09895"
     CDS             1211..1654
                     /gene="nudB"
                     /locus_tag="PLQ18_09895"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HNE02009.1"
                     /translation="MTASPWKRPESVLVVVHTRGGLVLLLRRLAPPDHWQSVTGSLEW
                     HESAPKVAARRELLEETGIDGGRLIDTGVVNRYPILPPWRARYAPEATENREYVFLLE
                     LAEPCPIRLCPEEHDAVVWLPRLAAAQQVFSYTNREAVLAFVPRQ"
     gene            1711..2289
                     /locus_tag="PLQ18_09900"
     CDS             1711..2289
                     /locus_tag="PLQ18_09900"
                     /inference="COORDINATES: protein motif:HMM:NF016836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HNE02010.1"
                     /translation="MNQHSSRSRLRSAIALALCLGVGGLTLAGCASTATSESAGEYVD
                     NAAITAKVKEKYLADPGIKSLKIEVATYKGVVQLTGFVESADQKARAEQLAASVQGVE
                     SVKNEIVVLRKGETAGQYMDSAAITAKVKAKIAEDPHLSAFAIEVETQRGIVQLSGFV
                     DSSAQRTAAGRIARSVEGVKSVKNSLTVKPRS"
     gene            complement(2396..>2625)
                     /locus_tag="PLQ18_09905"
     CDS             complement(2396..>2625)
                     /locus_tag="PLQ18_09905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02011.1"
                     /translation="LTATAPTPAREPRRERRPRPDGETPRRRADRGDRKEPRREREPV
                     RASAAPEQPVESAFASLLREALAKQDAPEKP"
BASE COUNT          398 a          941 c          918 g          368 t
ORIGIN      
        1 cgatgcctcc ggctacgccg ccaacgtcga actggccgag gcactggctc cggccggcga
       61 acgcccggcc gccggcgagg cgctgacgcg cgtgcacacg ccgaaggtca agaccatcgc
      121 cgagcttgcc gccttcctga agatcccggc cgaacgcacg ctgaaggccg tcgtcgtcga
      181 cggcagcgac ggccggccgg tgctgctgct gctgcgcggt gaccacgagc tgaacgagat
      241 caaggccggc aagctgccgg aagtggccga gccgctgacg ttctcgactc cggccgcgat
      301 cagcgccgcc ttcggcgccc agcccggctc gctcggcccg gtcggcttcg acggcgtggt
      361 catcgccgat cgcagcgccg ccgtgctcgc cgatgccgtg accggcgcca acgaggacga
      421 ctggcacctc agcggcttca acttcggccg cgactgcccg gaaccgcgcg tcgccgacct
      481 gcgcaacgtc gtcgccggcg acccgagccc ggacggccag ggccggcttt cgatccgccg
      541 cggcatcgag gtcggccaca tcttccagct cgggcgcaag tacagcgacg cgctcgccgc
      601 gaacgtgctc ggcgaggacg gccagaacct gaccgtgacc atgggctgct acggcatcgg
      661 cgtctcgcgc gtcgtcgccg ccgcgatcga acagaactgg gacgagcgcg gcatcctgtg
      721 gccggcggcg atggcgccgt tccagctcgc gctgctgccg atgaacgcgc acaagagcgc
      781 cgccgtgcgc gaggcggtcg agcagctcta cgccgactgc cgggccgccg gcatcgacgt
      841 gctgctcgat gaccgcgagg cgcgccccgg cgtgatgttc gcggacatgg agctgatcgg
      901 catcccgcac cgcgtcgtca tcggcgagaa ggggcttgcc gccggcagcg tcgagtacaa
      961 gggccggcgc gacgccgaag ccctcgccgt gccgcttgca ggtctcatcg acttcctgcg
     1021 cgagcgcctc ggcgcggcct gagggaagac ccttcccggg ccaggcagct gcgcgcagga
     1081 cgcgcgcctg ccgggtgcgc aggcccggcg ccccgagcgg gcgcgcgtca ggctcatcga
     1141 cgcctccgga gcggccgcaa tggccgcttc ccgggtcttc ccggataccc gcaacttttg
     1201 caggtgcccg atgaccgcct cgccatggaa acgccccgaa tccgtgctcg tcgtcgtgca
     1261 cacgcgcggc ggcctcgtgc tgctgctgcg ccgcctcgcc ccgcccgatc actggcagtc
     1321 ggtgaccggc agcctcgaat ggcacgagtc cgccccaaaa gttgcggccc ggcgcgaact
     1381 cctggaggaa accgggatcg atgggggcag gctgatcgat accggcgtcg tcaaccgcta
     1441 tccgatcctg ccgccgtggc gggcgcgcta cgcgcccgaa gccacggaaa accgggaata
     1501 cgtgtttctg ctcgaactcg cggaaccttg tccgatccgg ttgtgtcctg aggaacacga
     1561 cgctgtcgtc tggctgccac gtttggcagc agcgcaacag gtgttttcgt atacgaatcg
     1621 cgaggccgtg ctcgcgttcg tccccaggca gtgaaatcgc tgtccagctg cactccggca
     1681 gccatccaga acaacagaag gagaatcccg atgaaccagc actcgagccg ttcgcgcctg
     1741 cgttccgcca ttgcactggc cctctgcctc ggcgtcggtg gtctgaccct cgccggttgt
     1801 gccagcacgg ccaccagcga gagcgccggc gagtacgtcg acaacgccgc gatcaccgcc
     1861 aaggtcaagg agaagtacct ggccgatccg ggcatcaaga gcctgaagat cgaggttgcg
     1921 acctacaagg gcgtggtgca gctgaccggc ttcgtcgagt cggccgacca gaaggcgcgc
     1981 gccgagcagc tcgccgcgag cgtccagggc gtcgagtcgg tcaagaacga gatcgtcgtg
     2041 ctgagaaagg gcgagaccgc cggtcagtac atggacagcg ccgcgatcac cgccaaggtc
     2101 aaggcgaaga tcgccgagga tccgcacctc agcgccttcg cgatcgaggt cgagacgcag
     2161 cgcggcatcg tgcagttgag cggcttcgtc gacagctccg cgcagcgcac ggcagccggc
     2221 cgcatcgcgc gctcggtcga gggcgtgaag tcggtgaaga acagcctgac cgtcaagccg
     2281 cgcagctgag cggctgccgc gtcgcccgtc atcggggccc ggccggagcg atccggccgg
     2341 gccccggcgt ttttcagcgc ggctccctgc cgacatggcg ggagccgttg ccgcctcagg
     2401 gcttttccgg ggcgtcctgc ttggccagcg cctcgcgcag cagcgacgcg aacgcgctct
     2461 cgaccggctg ctcgggcgcg gcgctggcgc gcaccggttc gcgctcgcgg cgcggttcct
     2521 tgcgatcacc gcgatcggca cggcggcgcg gcgtctcgcc atccggacgc ggccggcgct
     2581 cgcggcgcgg ctcgcgggcg ggagtggggg cggtggcggt gaggg
//
LOCUS       DAOQJH010000434         2621 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_27964_length_2621_cov_2.932580, whole genome shotgun sequence.
ACCESSION   DAOQJH010000434 DAOQJH010000000
VERSION     DAOQJH010000434.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2621
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27964_length_2621_cov_2.932580"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..749
                     /locus_tag="PLQ18_09910"
     CDS             <1..749
                     /locus_tag="PLQ18_09910"
                     /inference="COORDINATES: protein motif:HMM:NF014193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SURF1 family cytochrome oxidase biogenesis
                     protein"
                     /protein_id="HNE02012.1"
                     /translation="KRAIALYRMSTGAASLTIAPAGAALLRAEQPNFYRQLALELRTG
                     DDIPLEDLLAHLDSIGYEKREPVELRGEYRTEHEFLLLNRVRDGRVGIEAYTPFVPAG
                     GGDWLLVNRGWYESPQARPKIPDAPPAGVQTIRGLLAQPANPGLRLGTIEAAGPWPQP
                     VVWLDAPAAAHALGHGVLGAVLLLDAGLPGGFVRDWHPDPGEFGPERHRGYALQWFAL
                     AVTVVCTWIVLSRRQRRRDSLPEARTSPSA"
     gene            746..1369
                     /locus_tag="PLQ18_09915"
     CDS             746..1369
                     /locus_tag="PLQ18_09915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome oxidase assembly protein"
                     /protein_id="HNE02013.1"
                     /translation="MNPELTLLQLRPASVRRARLQLLLIIALFALPLAVAIGLFGQWR
                     PAGSAEHGELLNPVRALSPLSALATDDTPLAPDFLHGRWTLVYVASGSDCPALCRDAL
                     WRLRQVRLAIGRDIDRVQRLALFASMPDDASRQWLAREHPGLAAGAATTEARTEFTGD
                     WRTDGRPGGWIYLVDPHGNLVLRYAADGEAGGMLKDLTRLLKLSQIG"
     gene            1375..2415
                     /locus_tag="PLQ18_09920"
                     /pseudo
     CDS             1375..2415
                     /locus_tag="PLQ18_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015259764.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
BASE COUNT          318 a          964 c          920 g          419 t
ORIGIN      
        1 aaaagcgcgc catcgccctc taccgcatga gcaccggcgc ggcctcgctc accattgccc
       61 ccgccggcgc cgccctgctg cgcgccgaac agcccaactt ctatcgccag ctcgccctcg
      121 aactccgcac cggcgacgac atcccgctcg aagacctcct cgcccacctc gacagcatcg
      181 gctacgagaa gcgcgagccc gtcgaactgc gcggcgaata ccggacggag cacgagttcc
      241 tgctgctgaa ccgcgtgcgc gacggtcgcg tcggcatcga ggcctacacg cccttcgtgc
      301 cggccggcgg cggtgactgg ctgctggtca accgcggctg gtacgagagt ccgcaggccc
      361 gcccgaagat ccccgacgct ccaccggccg gcgtgcagac gatccgcggc ctgctcgcgc
      421 agccggccaa tccgggcctg cgcctcggca cgatcgaggc ggccgggccg tggccgcagc
      481 cggtggtctg gctcgatgcc cccgccgcgg cgcacgcgct cgggcacggc gtgctcggag
      541 ccgtgctgct gctcgatgcc gggctgccgg gcggcttcgt ccgtgactgg catccggatc
      601 ccggcgagtt cggccccgag cgccaccgcg gctatgcgct gcagtggttc gcgctggcgg
      661 tgaccgtcgt ctgtacctgg atcgtcctga gccgccggca gcgccggcgc gattcccttc
      721 ccgaggcccg aacgagtccg tccgcatgaa tccggaattg accctgctgc agcttcgccc
      781 cgcgagtgtg cgtcgcgcgc gtctgcaact gctgctgatc atcgcgctgt tcgccttgcc
      841 gctggcggtg gcgatcggcc tgttcggcca gtggcgcccg gccggcagcg ccgagcacgg
      901 cgaactgctg aacccggtgc gcgcgctgtc accgctgtcg gcgctcgcca ccgacgacac
      961 gccgctcgcg ccggacttcc tgcacgggcg ctggacgctg gtgtatgtgg cctccggcag
     1021 cgactgcccg gcgctctgtc gcgatgcgct gtggcggctg cgtcaggtgc gcctcgccat
     1081 cggccgcgac atcgaccgcg tgcagcgcct ggcgctgttc gcctcgatgc ccgatgacgc
     1141 gagccgccag tggctggcgc gcgagcatcc ggggcttgca gccggcgcgg ccacgactga
     1201 ggcccgcacg gaattcaccg gggactggcg cacggacggg cgtcccggcg gctggatcta
     1261 cctcgtcgat ccgcacggca atctggtgtt gcgctacgcc gccgacggcg aggccggcgg
     1321 catgctgaag gacctgaccc gcttgttgaa gctctcgcag atcggctgat tcccatgcag
     1381 accctgtcgc ccgcagtttc cctgacctcc gccgcagcgc ctgccgtgcc gggccggcgg
     1441 ctggcgtttt cgcagctggc catcgcggcc tgcgtgctgg cgctgtgcgt ggtcgtgctc
     1501 ggtgcatatg tgcgcctgtc gcacgccggc ctcggctgtc cggactggcc gggctgctac
     1561 ggccacctcg atgtaccgga atcggtccgg cacatcgagg ccgccaacgc cgcgtggccg
     1621 gaccgcccgg tcgagccgga gaaggcctgg aaggagatga tccaccgcta tgcggccggc
     1681 acgctcgggc tgctgatcct cgcgctcgcc gtgagtgcct ggcgccggcg gcatgagccg
     1741 gggcagatgc tggccctgcc gctggcgctg gtcgccctgg tgctgttcca ggcgctgctc
     1801 ggcatgtgga ccgtgacgct gctgctgaag ccgctgatcg tgctcggaca cctgctcggc
     1861 gggctggcga cgctcgggct gctgttctgg ctggcgctgc gccagtccgg ctggcagccg
     1921 cgcccggccg aagccgaacg ccgccggctc ggcggctggg cgctgctggc gctcgtggtg
     1981 ctgatcgtgc agatcgcgct cggcggctgg accagcacca actatgcggc gctcgcctgc
     2041 ccggacctgc cgacctgtca gggccgctgg tggccgccga ccgatttcgc cgatgcctac
     2101 ctgccgtggc gcggactcgg catcaactac gagggcggcg tgctcgacaa cgaggcgcgc
     2161 atgaccatcc acgtcatgca ccgcgtcggc gcgctgctcg cactcgccgt gctcggcgcg
     2221 ctggcgtggc ggctggcgcg aagcctcgat gccggcctgc gccgcggcgg ctggctgctg
     2281 ttcgccgcgc tcgtggcgca gctgctgctc ggcctcggca acgtgctgct gtcgctgccg
     2341 ctgccggtcg cggtcgccca caacgccggg gcggccgtac tgctgctggt cgtgctcggc
     2401 ctgaaccacc tgctgcatca atggcatgca ggagaactgc ctgttcaggt cgtgggcgtc
     2461 gtatccaacc acgacgacat gcgccggctg gtcgagtggc acgatctgcc gtaccaccac
     2521 ctgccgatga gcgagggcaa ggccgctcag gaggcacttt tccgagaggt gatcgaggcg
     2581 agcgaggccg agctcaccgt gctggcgcgc tacatgcagg t
//
LOCUS       DAOQJH010000435         2612 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_28144_length_2612_cov_4.804458, whole genome shotgun sequence.
ACCESSION   DAOQJH010000435 DAOQJH010000000
VERSION     DAOQJH010000435.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2612
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28144_length_2612_cov_4.804458"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..408
                     /gene="pgk"
                     /locus_tag="PLQ18_09925"
     CDS             <1..408
                     /gene="pgk"
                     /locus_tag="PLQ18_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HNE02014.1"
                     /translation="LPVDVVVADELSALARANQVAADAVQPGDMILDIGPKSAAQLAY
                     IVAHAGTIVWNGPVGVFEYDQFGGGSKMLASAIAHSDAFSIAGGGDTLAAIAKYRIAD
                     QVGYISTGGGAFLEFLEGKPLPAITVLEARAAG"
     gene            512..1576
                     /gene="fba"
                     /locus_tag="PLQ18_09930"
     CDS             512..1576
                     /gene="fba"
                     /locus_tag="PLQ18_09930"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249246.1"
                     /note="catalyzes the reversible aldol condensation of
                     dihydroxyacetonephosphate and glyceraldehyde 3-phosphate
                     in the Calvin cycle, glycolysis, and/or gluconeogenesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase class II"
                     /protein_id="HNE02015.1"
                     /translation="MALISMRQLLDHAAEHGYGVPAFNVNNLEQMRAIMEAAAQTDSP
                     VIVQASAGARKYAGAPFLRHLILAAIEEFPEIPVCMHQDHGTSPAVCQRSIQLGFSSV
                     MMDGSLGEDGKTPTSYDYNVEVTRQTVAMAHACGVSVEGELGCLGSLETGMAGEEDGI
                     GAEGMLDHSQLLTDPEEAAQFVKATKVDALAIAIGTSHGAYKFTRPPTGDVLAISRIK
                     EIHARIPNTHLVMHGSSSVPQDWLAIINEYGGDLGQTYGVPVSEIVEGIRHGVRKVNI
                     DTDLRMASTGTIRRFLARNPKEFDPRKYLGETVKAMKGICVERYEAFGTAGQASKIRA
                     LSLEAMTKRYASGELDPQVH"
     gene            1652..2224
                     /locus_tag="PLQ18_09935"
     CDS             1652..2224
                     /locus_tag="PLQ18_09935"
                     /inference="COORDINATES: protein motif:HMM:NF017496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HNE02016.1"
                     /translation="MTVVAERRPFTADEFLVWEAQQALRHEYLAGDVFAMTGGTLDHN
                     RLTRNLLMALCRRLDGTPCEAFSSDVMLRVEAADAYFYPDVIVTCSEADRRAERVISE
                     PRVVAEVLSPSTAAHDWGAKFEAYSHLPGLAEYVLVEAERIAVHVLRPNAAQRWERWH
                     CGAGDSLELTSLGLDIPLAELYAGTASAGG"
     gene            2350..>2612
                     /locus_tag="PLQ18_09940"
     CDS             2350..>2612
                     /locus_tag="PLQ18_09940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02017.1"
                     /translation="MNAATDTAVRDFFDWQDRLAQRVRPLDEARVHGQLITRLQALLR
                     ERLRGLPARVWAPGPAVHLAEGARTVHPDLAVSSDEADLCSGLP"
BASE COUNT          431 a          930 c          873 g          378 t
ORIGIN      
        1 ctgccggtcg acgtggtcgt cgccgatgaa ctctcggcgc tcgcgcgtgc caatcaggtg
       61 gcggccgatg ccgtgcagcc cggcgacatg atcctcgaca tcggcccgaa gtcggccgcg
      121 cagctcgcgt acatcgtcgc gcacgccggc accatcgtct ggaacggacc cgtaggcgtg
      181 ttcgagtacg accagttcgg cggcggctcc aagatgctcg cctcggcgat cgcgcactcc
      241 gatgccttct cgatcgccgg cggcggcgat acgctggccg cgatcgccaa gtaccggatc
      301 gccgatcagg tcggctacat ctcgaccggc ggcggcgcct tcctggagtt cctcgaaggc
      361 aagccgctgc cggcgatcac ggtgctcgaa gcccgcgcag ccggctgagg cgcgccgtca
      421 ctacacaatg gcgccgtcgc gacggcggcg ccggcaaaac caccgcagga tcgacccagc
      481 aaggggcagg cccgccccgg gagcacgaac gatggccctg atttcgatgc gtcagctgct
      541 ggatcacgcc gccgaacacg gctacggcgt cccggccttc aacgtcaaca acctcgaaca
      601 gatgcgcgcg atcatggaag ccgccgcgca gaccgacagc ccggtgatcg tccaggcctc
      661 ggccggcgcc cgcaagtacg ccggcgcgcc gttccttcgg cacctgatcc tcgccgcgat
      721 cgaggaattc ccggagattc cggtctgcat gcatcaggat cacggcacct cgccggcggt
      781 ctgccagcgc tcgatccagc tcggattttc ctcggtgatg atggacggct cgctcggcga
      841 ggacggcaag accccgacca gctacgacta caacgtcgag gtcacccgcc agaccgtggc
      901 gatggcgcac gcctgcggcg tgtcggtcga gggcgagctc ggctgcctcg gctcgctcga
      961 aaccggcatg gccggcgagg aggacggcat cggtgccgag ggcatgctcg atcactcgca
     1021 gctgctcacc gatccggagg aagccgcgca gttcgtgaag gccaccaagg tcgacgcact
     1081 ggcgatcgcg atcggcacca gccacggcgc ctacaagttc acgcgcccgc cgaccggcga
     1141 cgtgctggcg atctcgcgca tcaaggagat ccacgcgcgc atcccgaaca cgcatctggt
     1201 catgcacggc tcgtcgagcg tgccgcagga ctggctcgcg atcatcaacg agtacggcgg
     1261 cgacctcggc cagacctacg gcgtgccggt ttccgagatc gtcgagggca tccgtcacgg
     1321 cgtgcgcaag gtcaacatcg acaccgacct gcgcatggcc tcgaccggca cgatccgccg
     1381 cttcctcgcc cgcaacccga aggagttcga cccgcgcaag tacctcggcg agacggtcaa
     1441 ggcgatgaag ggcatctgcg tcgagcgcta cgaagccttc ggcaccgccg gccaggcctc
     1501 gaagatccgg gcgctgtcgc tggaggcgat gacgaagcgc tacgccagcg gcgagctcga
     1561 tccgcaggtg cactgaggct gcgacgccgg tcgcatgcca gcgcgaccgg cgttagcatc
     1621 gcaagtcccg aaccactgcg ggaacgcccc gatgactgtt gtcgccgagc gccggccgtt
     1681 cacggccgat gaattcctcg tctgggaagc gcagcaggcg ctgcgccacg agtatctggc
     1741 cggcgacgtg ttcgcgatga ccgggggcac gctcgatcac aaccggctga cacgcaacct
     1801 gctgatggcc ttgtgcagac ggctggacgg tacgccctgc gaggcgtttt cgagcgatgt
     1861 gatgctgcgc gtcgaagcag ccgatgcgta tttctatccc gacgtcatcg tcacctgcag
     1921 cgaagccgat cgtcgtgccg agcgcgtgat ctcggaaccg cgagtcgttg ccgaggtgct
     1981 gtcaccgagc acggcggccc atgactgggg cgcgaagttc gaggcttaca gccatctgcc
     2041 cggtctcgcc gagtacgtgc tggtcgaggc cgagcgcatc gccgtgcacg tgctgcgtcc
     2101 gaacgccgcg cagcgctggg agcgctggca ctgcggggcg ggcgacagcc tggaactgac
     2161 gagtctcggc ctcgacatcc cgctcgccga actctacgcc ggcaccgcat cggccggcgg
     2221 ctgacaccgg atcgtcatcc cgctgctgca tcgtcgatcc cgcatcccga aaccagcgcc
     2281 gcgaccgccg gacggtccgc ggtccgtcat cgaccggccc gagggccgcg acaggagtgc
     2341 aagcccgtca tgaacgccgc caccgacacc gccgtacggg acttcttcga ctggcaggac
     2401 cgcctcgcgc agcgcgtgcg gccgctcgac gaggcccgcg tgcatgggca gctgatcacg
     2461 cgcctgcagg cgctgctgcg cgaacggctg cgcgggctgc cggcgcgggt ctgggcgccg
     2521 gggccggccg tgcatctggc cgaaggcgcg cgcaccgtgc atccggacct cgcagtcagc
     2581 agcgacgagg ccgatctctg ctcgggcctg cc
//
LOCUS       DAOQJH010000436         2612 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_28145_length_2612_cov_3.588189, whole genome shotgun sequence.
ACCESSION   DAOQJH010000436 DAOQJH010000000
VERSION     DAOQJH010000436.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2612
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28145_length_2612_cov_3.588189"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..610)
                     /locus_tag="PLQ18_09945"
     CDS             complement(<1..610)
                     /locus_tag="PLQ18_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020558494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Era-like GTP-binding protein"
                     /protein_id="HNE02018.1"
                     /translation="MNIWKRARDGWPHWWSAVLDRLNPAATPTAATPEPGAPGMAGPS
                     ASAADTHLGLARDSLRALLADEAVPAAVRERLAEDYSALSLLLERLEHGHLHIAVFGR
                     VSVGKSALLNALLGEQRFATSPLHGETTAASSARWAADPELPGVQRLEDGGVFLIDTP
                     GIDEVGGEARERLAREVAGGADLILFVCEGDLTATELNALREL"
     gene            complement(510..1982)
                     /locus_tag="PLQ18_09950"
     CDS             complement(510..1982)
                     /locus_tag="PLQ18_09950"
                     /inference="COORDINATES: protein motif:HMM:NF014474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HNE02019.1"
                     /translation="MRRWRPLLFALGLALTLALALHVTDFALTLWQRLSEASPLFLTL
                     YGLLIALVAWLVLRLMRPRRPVAAAAPPPPDAPQIDRQLEAALQAGLPVERYRAELAE
                     LERRRAGGRVIVACFGEVSAGKSSLIRALLPGAEVVVSPVGGSTRTIVHHVWTSPAGD
                     ELVLADVPGLNEADGTLDAEAEGEAVRAHAVIYVTGGDLARAELAALERLLSLGKPLL
                     VALNKADRYTAAELALVQARIRERLAGRAELVAVSAGGTEEVVRIGADGSETRIERER
                     PADVHALGVALQRTLDADAGALESLRDAAVFTLIGRELDAALAAERAARAETVVETYA
                     RKAVFGALAAVGPGTDVLIQGWLGMQMLKELSAVYGVPAQEIDLKRFLDLASRHVGRR
                     MNLLLALAGNVLKAFPGVGTVLGGALHAIAYGLIFESLGHAAARSLASRGALVPGVAV
                     HLFEDALGEHLETRARRLAALVVGRARSAEPGRDADRSDA"
     gene            complement(1990..2427)
                     /locus_tag="PLQ18_09955"
     CDS             complement(1990..2427)
                     /locus_tag="PLQ18_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011629200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HNE02020.1"
                     /translation="MARVKLDLPATFAFRTSMQVRVGDINYGRHLGNDALLGLLHEAR
                     VRWLAAHGQSELDCWGAGLIMVDSVIVYRAEAFLGETLLFDVATADVHGKGCDLVYRV
                     SAAGDGRDIAHAKTGILFFDYATRRVRAMPEEFRSALCAAAGA"
BASE COUNT          383 a          944 c          975 g          310 t
ORIGIN      
        1 cgagttcgcg cagcgcgttg agctcggtcg cggtcaggtc gccctcgcag acgaacagga
       61 tcaggtcggc gccgccggcg acctcgcgtg ccagccgctc gcgcgcctcg ccgccgacct
      121 cgtcgatgcc gggcgtgtcg atcaggaaca cgccgccatc ctccagccgc tgcacgccgg
      181 gcagctccgg atcggccgcc cagcgcgccg agcttgcggc cgtggtctcg ccgtgcagcg
      241 gactggtcgc gaagcgctgc tcgccgagca gggcgttcag cagcgccgac ttgccgacgc
      301 tgacccggcc gaacacggcg atgtgcagat ggccgtgttc gaggcgttcg agcagcagcg
      361 acagcgccga gtaatcctcg gccaggcgct cgcgcacggc cgccggcacg gcctcgtcgg
      421 cgagcagcgc gcgcaggctg tcgcgcgcga gaccgaggtg cgtgtcggcc gcggacgcgg
      481 aagggccggc catcccggga gcgccgggct caggcgtcgc tgcggtcggc gtcgcggccg
      541 ggttcagccg atcgagcacg gccgaccacc agtgcggcca gccgtcgcgc gcgcgtttcc
      601 agatgttcac cgagggcatc ctcgaacagg tgtaccgcca cgcccggcac cagcgcaccg
      661 cggctcgcga gcgagcgggc ggcggcgtga cccaggctct cgaagatcag accgtaggcg
      721 atcgcatgca gtgcgccgcc gagcacggtg ccgacgccgg gaaaggcctt cagcacgttg
      781 ccggccagcg ccagcagcag gttcatgcgc cgcccgacgt ggcggctggc gagatcgagg
      841 aagcgcttca ggtcgatctc ctgtgccggc acaccgtaga cggccgaaag ctccttcagc
      901 atctgcatgc cgagccagcc ctggatcagc acgtccgtgc ccggcccgac cgcggccagc
      961 gcgccgaaca cggccttgcg cgcgtaggtc tcgacgacgg tctcggcgcg cgcggcgcgc
     1021 tcggcggcaa gggctgcgtc gagttcgcgc ccgatcagcg tgaacaccgc ggcatcgcgc
     1081 agggattcga gcgcaccggc atcggcatcg agcgtgcgct gcagcgccac gccgagcgcg
     1141 tgcacgtcgg ccgggcgctc acgctcgatg cgcgtctcgc tgccgtcggc gccgatgcgc
     1201 acgacttcct cggtaccacc ggcactgacc gcgacgagct cggcccgtcc ggccagccgc
     1261 tcgcggatgc gcgcctgcac cagcgcgagt tccgcggcgg tgtagcgatc ggccttgttc
     1321 agcgccacca gcagcggctt gcccagactc agcaggcgct cgagcgcggc gagttcggcg
     1381 cgcgcgagat cgccgccggt cacgtagatc accgcgtgcg cacgcacggc ctcgccctcg
     1441 gcctcggcat cgagcgtgcc gtcagcctcg ttgaggccgg gcacgtcggc gagcacgagt
     1501 tcgtccccgg ccgggctggt ccagacgtga tggacgatcg tgcgcgtgct gccgccgacc
     1561 ggcgagacca cgacctcggc gcccggcagc agcgcgcgga tcaggctgga cttgccggcc
     1621 gagacctcgc cgaaacaggc gacgatcacg cgcccgcccg cgcgccggcg ttcgagttcg
     1681 gcgagctcgg cgcggtagcg ctcgaccggc agcccggcct gcagggcggc ttcgagctgg
     1741 cggtcgatct gcggggcgtc gggcggcggc ggcgcggcgg ccgcgaccgg ccggcgcgga
     1801 cgcatcaggc gcagcaccag ccacgcgacc agcgcgatca gcagcccgta cagcgtcagg
     1861 aacagcgggg atgcctccga caggcgctgc cacagcgtca gcgcgaagtc ggtgacgtgc
     1921 agcgccagcg ccagggtcag cgccagcccg agcgcgaaca gcagcggtcg ccagcgacgc
     1981 atgcgcgact caggccccgg cggcggcgca gagcgccgag cggaattcct ccggcatcgc
     2041 gcgcacgcgc cgcgtcgcgt agtcgaagaa caggatgccg gtcttggcgt gcgcgatgtc
     2101 gcggccatcg ccagcggcac tgacgcggta gacgaggtcg cagcccttgc cgtggacatc
     2161 ggcggtggcg acatcgaaca gcagcgtctc gccgaggaag gcctcggcgc ggtagacgat
     2221 gacggaatcg accatgatca gccccgcccc ccagcagtcg agttcgctct ggccgtgcgc
     2281 ggccagccag cgcacgcgcg cctcgtgcag caggccgagc agcgcgtcgt tgccgaggtg
     2341 gcggccgtag ttgatgtcac cgacgcgcac ctgcatcgag gtgcggaagg cgaaggtcgc
     2401 gggcaggtcg agcttgacgc gggccatgcg gtcacctcgg ggcgcggtgg atgaagcgtg
     2461 gacggggggg cggaaatgcg aacgccgggc ggcttgcgac cgcccggcgt ccgggacggg
     2521 aaacgacggc gcccaggcgg tgcaggccgc gaacaggcgc tcctccagcg cggtgcgcgc
     2581 ggcaagcgtg gcaccgaggc gcgcgagatc gg
//
LOCUS       DAOQJH010000437         2609 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_28226_length_2609_cov_2.467502, whole genome shotgun sequence.
ACCESSION   DAOQJH010000437 DAOQJH010000000
VERSION     DAOQJH010000437.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2609)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2609)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2609
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28226_length_2609_cov_2.467502"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2189)
                     /locus_tag="PLQ18_09960"
     CDS             complement(<1..2189)
                     /locus_tag="PLQ18_09960"
                     /inference="COORDINATES: protein motif:HMM:NF037694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HNE02021.1"
                     /translation="MRALPLLCLLFAWLSAMPAHAFEDKTLGVAADQYRAALIAQAGK
                     VLPPDTQAKADAALKAKKWGEAIVPLELLVRAHPDDGPLWLKLARARGNSNPQDERAR
                     PAAYAALRLAKSAEEQAAALFVLGRLTEVNQDGRAALAMYDEGLALVPDEGIARRAAN
                     LRATLIIKPVNAAVEEESATPRFCVEFSDPLSKARQYHYEDFLQTEPAVRLSVTVRDT
                     KLCAEGVEWGQGYRVTVRAGVPGAKGDRTASSETFTLAFPDRKPSVAFRGNGYVLPRK
                     AGQMLPVVSVNLPAAELKLLRINDRNLIREVLNGSFDEALYGSDEDAIASDRGEEVWH
                     GSVEFAGDKNRETVTLLPLKTVLPETKPGVYVLTARPPRKQAPAAGDEDDDYDYDVHA
                     TQWLIVSDLGLSVLRGADGLHVFVRSLESGRPAPNVELRLLARNNTELATVTSDAEGR
                     AKFDPGLLRASGGRAVRGLYAYGPGGDFNLLDLGQPAFDLSDRGVDGRVQPVGLDAFL
                     YTERGVYRPGEAVELSALLRDAGASAVENLPVTLIVRRPDGEEAERRVLAPGAPGAYH
                     TRLELPANARSGTWTARLHAEPDGPAIGERRFQVEDFVPQQIKVELATDQPFLTPDSA
                     VVLAISGQFLYGAPAAGLPVEMDATLEADPDPYPQFAGYRFGLVQDEWEPAYQNLEGL
                     PQATDEEGRAVARIEAPPVPDTGRPLRLSVRVGIEEPGGRPVRRVVRL"
BASE COUNT          416 a          910 c          880 g          403 t
ORIGIN      
        1 aggcgcacga cgcggcgcac cggccgtccg cccggttcct cgatgccgac gcgcacgctc
       61 aggcgcagcg gccggccggt atcgggcacc ggcggcgcct cgatgcgcgc caccgcccgg
      121 ccctcctcgt cggtagcctg cggcagacct tccaggttct ggtaggccgg ttcccattcg
      181 tcctgcacca gtccgaagcg gtagcccgcg aactgcggat acggatcggg atcggcttcg
      241 agcgtcgcgt ccatctcgac cggcaggccg gcggccggcg cgccgtacag gaactggccg
      301 ctgatcgcga gcacgaccgc cgaatccggc gtcaggaacg gctggtcggt ggcgagttcc
      361 accttgatct gctgcggcac gaagtcctcg acctggaagc gccgctcgcc gatcgccggc
      421 ccgtccggct cggcgtgcag gcgcgcggtc caggtgccgc tgcgggcgtt ggccggcagt
      481 tcgaggcggg tgtgatacgc gcccggtgcg ccgggggcga gcacgcgccg ctcggcctcc
      541 tcgccgtcgg ggcggcgcac gatcagcgtc accggcaggt tctcgaccgc gctcgcgccg
      601 gcatcgcgca gcagcgcgct cagctccacc gcctcgccgg gccggtagac gccgcgctcc
      661 gtatacagga aggcatcgag cccgaccggc tgcacacggc catcgacgcc gcgatcggac
      721 agatcaaacg ccggctggcc gagatcgagc aggttgaagt caccgcccgg cccgtaggca
      781 tagaggccgc gcaccgcgcg cccgccgctg gcgcgcagca ggccgggatc gaacttcgcg
      841 cgcccctcgg catcgctggt cacggtcgcg agttcggtgt tgttgcgcgc cagcaggcgc
      901 agttcgacgt tcggcgccgg ccggccggat tcgagcgaac gcacgaacac gtgcaggcca
      961 tcggcgccgc gcagcacgct caggccgagg tccgagacga tcagccactg cgtcgcgtgc
     1021 acgtcgtagt cgtagtcatc gtcctcgtcg ccggcggcgg gggcctgctt gcgcggcggg
     1081 cgggcggtca gcacgtagac gccgggtttc gtctccggca gcacggtctt gagcggcagc
     1141 agcgtcaccg tctcgcggtt cttgtcgccg gcgaactcga cgctgccgtg ccagacctcc
     1201 tcgccgcggt cgctggcgat cgcatcctcg tcggagccgt acagcgcctc gtcgaagctg
     1261 ccgttcagca cttcgcggat caggttgcga tcgttgatgc gcagcagctt cagttccgcc
     1321 gccggcaggt tgacgctgac caccggcagc atctgcccgg ccttgcgcgg cagcacgtag
     1381 ccgttgccac ggaaggccac gctcggcttg cggtccggga acgccagcgt gaaggtctcg
     1441 ctgctggccg tgcgatcgcc tttcgccccc ggcacgccgg cgcgcaccgt cacccggtag
     1501 ccttgtcccc actcgacacc ctcggcgcag agcttggtgt cgcgcaccgt caccgacagc
     1561 cgcaccgccg gctcggtctg caggaagtcc tcgtagtggt actggcgggc cttcgagagc
     1621 gggtcgctga attcgacgca gaagcgcggc gtcgcggact cttcctccac cgccgcgttg
     1681 accggcttga tgatgagtgt cgcgcgcagg ttggcggcgc gcctggcgat gccctcgtcc
     1741 ggcaccagcg ccagcccctc gtcatacatc gcgagcgccg cgcgcccgtc ctgattaacc
     1801 tcggtcagcc gcccgagcac gaacagcgcc gccgcctgtt cctcggcgct cttggcgagg
     1861 cgcagcgccg catacgccgc cggccgcgcg cgctcgtcct gcggattgct gttgccgcgg
     1921 gcgcgcgcga gcttcagcca cagcggcccg tcatcggggt gcgcgcgcac cagcagctca
     1981 agcggcacga tcgcctcccc ccacttcttc gccttcaacg ccgcatcggc cttcgcctgc
     2041 gtatccggcg gcagcacctt gccggcctgc gcgatcagcg ccgcccggta ctgatcggcc
     2101 gcgacgccga gggtcttgtc ttcaaaggca tgggcaggca tggcggacag ccacgcaaac
     2161 agcaggcaca gcagcggcag ggcacgcatg gtgaagggat tccctgggga gcgtatggac
     2221 agaagcggag gatgccgcgc aggggcggga cggcgggtaa ggataggcgg tcgggacggg
     2281 ggcgggagta cggcgggtga cgggtggaaa ttgtcgcgga ctgggaccag tatcgggact
     2341 cggtgccgag acaaggatcc ggtgatgtca attgaataca atttgaccgt ctatgaaagt
     2401 gtggcgaatt agagctattc cgaagaaata tcggaactgc ccaaccgctt ggcgggcgat
     2461 accagttaca agggcatgca tttacacccc ctaacttggc tcaacacttt accaaaccaa
     2521 acacacataa ccatttatac aggaaaacag ccatgaacaa tcagcggaac aacatcagca
     2581 aaacgatcgg cacaattgtt ttgtcaata
//
LOCUS       DAOQJH010000438         2603 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_28322_length_2603_cov_3.408163, whole genome shotgun sequence.
ACCESSION   DAOQJH010000438 DAOQJH010000000
VERSION     DAOQJH010000438.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2603)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2603)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2603
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28322_length_2603_cov_3.408163"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..548
                     /locus_tag="PLQ18_09965"
     CDS             <1..548
                     /locus_tag="PLQ18_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014249644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNE02022.1"
                     /translation="SGFDVGARLRAVREANGLSQRELARRAGVTNGTISLIEQNKNSP
                     SVSSLKKVLDGIPMTLAEFFAQDEPPQDQVFFRAAELVTLTRGAVSFRQVGARAGHAL
                     QILHEHYAPGADTGRAMLRHDGEEGGIVLRGRIELTVGDRREILGPGDAYHFSSRIPH
                     RFRNPGDEECELISACTPPYL"
     gene            742..2439
                     /locus_tag="PLQ18_09970"
     CDS             742..2439
                     /locus_tag="PLQ18_09970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018411571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNE02023.1"
                     /translation="MNDPLSQTTEFLRGIEAALDPFGIGRACRETVTAWLQQPQQLAE
                     SMARLSADVLRVQAHAWKKALGVESDDVEPVSGFDERFQDPAWTAHPGFDALKEFYLL
                     QTHWLQDAIFATPGLDPASVQRAAFWVRQVLDAIAPTNFFWTNPLAIAKAIESGGTTL
                     RQGLTHFARDLAAGDVLMVEPDAFELGRELAATEGVVVFRNELLEVIQYTAQTPQVHA
                     MPVVLVAPWINKFYILDLKPRTSLVRHLVSQGFTVFVTSWRNAGAEARDTGFEDYLMH
                     GVRAAIDTARAITGSPEVHAVGYCLGGTALSTLMAWYAAGDEPCPVAHWTLFTTLTDF
                     SDPGEIGVFLTPETVDFIERNMQRKGYLDGAEMARSFRLLRPNSLIWHYVVHSYLYGA
                     ELPPFDVLFWNMDTTRMPATMHAWYLRELYLGNRLAKGEITLGGRRLQLGRVQQPLYA
                     VGCEQDHIAPWKQTFLTCGLVGGPVRYVLATSGHILGIINPPVDPPKRRYWVGDASGC
                     SDGPAWLTGVAKVPGSWWEDWVPWLAARCGELRELPPLGTAEYPVLAAAPGTYVLQK"
BASE COUNT          400 a          946 c          867 g          390 t
ORIGIN      
        1 acagcggctt cgatgtcggc gcacggctgc gcgcggtgcg cgaggccaac ggcctgtcgc
       61 agcgcgaact ggcgcgccgc gccggcgtca ccaacggcac gatctcgctg atcgagcaga
      121 acaagaacag cccctcggtc tcgtcgctga agaaggtgct cgacggcatc ccgatgacgc
      181 tcgccgagtt cttcgcgcag gacgaaccgc cgcaggacca ggtgttcttc cgcgccgccg
      241 agctggtgac gctgacgcgc ggtgccgtgt ccttccgcca ggtcggggcc cgcgccggcc
      301 acgcgctgca gatcctgcac gagcactacg cgcccggggc cgacaccggc cgcgcgatgc
      361 tgcgccacga cggcgaggaa ggcggcatcg tgctgcgcgg acgcatcgag ctgacggtcg
      421 gcgaccggcg cgagattctc ggccccggcg acgcgtacca cttctccagc cgcatcccgc
      481 atcgcttccg caacccgggc gacgaggaat gcgagctgat ctcggcctgc acgccgccgt
      541 atctgtgaga tttgcgagat ctgcgcgggt ctccgtgagc cgggcgcgcg actttctgta
      601 aacatcgtgc gcgcgccgcc gatgacagcc ggaatgaggg cctgacggct cgccggccac
      661 gaccgcggcc tctacgctct gtgccccgca atcccgcacg tgccaccggg ttgattccgc
      721 ccggttccgg agccgtcgct gatgaacgat cccctgtccc agaccaccga gttcctgcgc
      781 ggcatcgaag ccgcactcga tccgttcggc atcggccgtg cctgtcgcga gacggtcacg
      841 gcctggctgc agcagccgca gcagctcgcc gagtcgatgg cgcgcctcag tgccgacgtg
      901 ctgcgcgtgc aggcgcacgc ctggaagaag gcgctcggcg tcgagtccga tgatgtcgag
      961 ccggtctcgg gtttcgacga gcgctttcag gacccggcct ggaccgcaca tccgggcttc
     1021 gatgcgctga aggagttcta cctgctgcag acgcactggc tgcaggacgc gatcttcgcc
     1081 acccccggcc tcgatccggc ctcggtgcag cgcgcggcgt tctgggtgcg gcaggtgctc
     1141 gatgcgatcg cgccgaccaa cttcttctgg accaatccgc tcgcgatcgc caaggccatc
     1201 gaaagcggcg gcacgacgct gcgccagggc ctgacccact tcgcccgcga cctcgccgcc
     1261 ggcgacgtgc tgatggtcga gcccgatgcc ttcgaactcg gccgcgagct ggccgccacc
     1321 gaaggcgtcg tggtgttccg caacgagctg ctggaagtga tccagtacac ggcgcagacg
     1381 ccgcaggtcc acgcgatgcc ggtcgtgctg gtcgcaccgt ggatcaacaa gttctacatc
     1441 ctcgacctga agccgcgcac cagcctcgtg cgccatctgg tatcccaggg cttcacggtg
     1501 ttcgtgacca gctggcgcaa cgccggcgcc gaggcacgcg acaccggctt cgaggactac
     1561 ctgatgcacg gcgtgcgcgc ggcgatcgac accgcgcgcg cgatcaccgg ctcgcccgaa
     1621 gtgcacgcgg tcggctactg cctcggcggc acggcgctgt cgacgctgat ggcctggtac
     1681 gccgccggcg acgagccctg cccggtcgcg cactggacgc tgttcacgac gctgaccgac
     1741 ttctccgacc ccggcgagat cggcgtcttc ctgacgccgg aaaccgtgga cttcatcgaa
     1801 cgaaacatgc agcgcaaggg ctatctcgac ggcgccgaga tggcgcgcag cttccggctg
     1861 ctgcgcccga acagcctgat ctggcactac gtcgtgcaca gctacctgta cggcgccgag
     1921 ctgccgccgt tcgacgtgct gttctggaac atggacacga cgcgcatgcc ggcgacgatg
     1981 cacgcctggt acctgcgcga gctgtacctc ggcaaccggc tcgcgaaggg cgagatcacg
     2041 ctcggcggcc ggcggctgca gctcgggcgc gtgcagcagc cgctgtacgc ggtcggctgc
     2101 gagcaggacc acatcgcgcc gtggaagcag accttcctca cctgcggcct ggtcggcgga
     2161 ccggtgcgct acgtgctcgc gacctccggc cacatcctcg gcatcatcaa tccgccggtc
     2221 gatccgccga agcgccgcta ctgggtcggc gacgccagcg gctgcagcga cggcccggcc
     2281 tggctcaccg gcgtcgccaa ggtgccgggc tcgtggtggg aggactgggt gccgtggctc
     2341 gccgcacgct gcggcgaact gcgcgaactg ccaccgctcg gcaccgccga atatccggtg
     2401 ctcgctgcag cgcccggcac ctacgtgctg cagaagtagc cgcgcacgca ctcacggctc
     2461 aacggcggca ggggatttcg cagcccctgc acgcccgcga ctgatccgcc tcagcgcccg
     2521 agaaaacggc gcagacgctc cagcgcgagt tcgatgcgat ccagcccggt cgtgtacgcg
     2581 aagcgcacat gcgtcgcggc ctc
//
LOCUS       DAOQJH010000439         2592 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_28522_length_2592_cov_4.489160, whole genome shotgun sequence.
ACCESSION   DAOQJH010000439 DAOQJH010000000
VERSION     DAOQJH010000439.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2592
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28522_length_2592_cov_4.489160"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..771)
                     /gene="lgt"
                     /locus_tag="PLQ18_09975"
     CDS             complement(<1..771)
                     /gene="lgt"
                     /locus_tag="PLQ18_09975"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020482397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HNE02024.1"
                     /translation="MDVLIWSVDPEIVRFGGFTLRWYGVLFATAFLAGYEIMRRIYHA
                     EGKPEDELDTLLIYMMLGTVLGARLGHVLFYDPAYYLAHPLRILAVWEGGLASHGGAI
                     GIFLALWLYTRRRPDQPYLWLLDRLSIPTAFGGACIRTGNLFNSEILGNPSELPWAVV
                     FARVDVIPRHPVQVYEALAYLVIFLGLYALYRRTRRATPEGRLLGSFLVAVFGVRFLL
                     EFLKTPQAAYESGFAITVGQWLSLPLIAVGVWLLLRSRR"
     gene            complement(777..1856)
                     /locus_tag="PLQ18_09980"
     CDS             complement(777..1856)
                     /locus_tag="PLQ18_09980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005224996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HNE02025.1"
                     /translation="MNFVLAWFRRVFSDAQAVSLIIVIVTLVAIVLLLGRMLAPVLAG
                     IVIAYLLEGLVAKLERTGLQRWPAVLIVFFGFMGLVGFALTGPLPLLSEQATQLARQL
                     PDMLLKAQKYLLSLPEQYPRVVSEQQIADAIAMVKREIAGVGQNLLTWSLASVLSVIT
                     VIVYLILVPVLVFFFLKDKVPIVRWCRQFLPADTRLAGRVWDDVDRQIGNYVRGKVWE
                     VLVNWVICYLVFAWLGLQYAMLLGFMVGLSVIVPYIGAAVVTVPVALVGLFQWGWGPD
                     FMWLIGAYAILMVLDGNVLVPWLFSEVVDLHPVAIIVAVLTFGGLWGFWGVFFAIPLA
                     TVVQAVLKIWPKAVVVAVPGHGGVS"
     gene            2071..>2592
                     /locus_tag="PLQ18_09985"
     CDS             2071..>2592
                     /locus_tag="PLQ18_09985"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNE02026.1"
                     /translation="MNRPPETPLPAHAHAELLQRLQALIAYVSRRRQVEGDLTQIAAD
                     TASMFGAEACSIMLFKDGGSELRLHLTARSRPLPKELRGQGLSEAVGEGLAGRVAASG
                     RALLVADLATSEFAGISRHHTGSCICAPIPVAGRVRGVLNLSRPQPFTHDDLALACIA
                     GLLLGLALELARIQ"
BASE COUNT          486 a          932 c          858 g          316 t
ORIGIN      
        1 ccgccgcgaa cgcagcagca gccagacgcc gaccgcgatc agcggcaggc tcagccactg
       61 tccgaccgtg atcgcgaagc cgctctcgta ggcggcctgc ggcgtcttca ggaattcgag
      121 caggaagcgc acgccgaaca ccgcgaccag gaagctgccg agcagccggc cctcgggcgt
      181 ggcgcggcgc gtgcgccggt acagcgcata cagcccgagg aagatcacga gatacgcgag
      241 cgcctcgtag acctgcaccg ggtggcgcgg gatcacgtcg acgcgcgcga acaccacggc
      301 ccacggcagc tcgctcgggt tgccgaggat ctcggagttg aacaggttgc cggtgcggat
      361 gcaggcaccg ccgaacgcgg tcgggatgct caggcgatcg agcagccaca gatacggctg
      421 gtcgggccgc cggcgcgtgt acagccagag cgccaggaag atgccgatcg cgccgccgtg
      481 gctcgcgagc ccgccctccc agaccgcgag gatgcgcagc ggatgcgcga ggtaataggc
      541 cgggtcgtag aacagcacgt gcccgagccg ggcgccgagc acggtgccga gcatcatgta
      601 gatcagcagc gtgtcgagtt cgtcctcggg cttgccctcg gcgtgataga tgcggcgcat
      661 gatctcgtaa ccggccagga aggccgtcgc gaacagcacg ccataccagc gcagcgtgaa
      721 gccaccgaac cggacaatct cggggtcgac gctccagatc agcacgtcca tcggaatcag
      781 ctcactccgc cgtgcccggg cacggccacg acaaccgcct tcggccagat cttcagcacc
      841 gcctgcacga cggtcgccag cgggatcgcg aagaacacgc cccagaaccc ccacaggccg
      901 ccgaaggtca gcaccgcgac gatgatcgcc accgggtgca ggtcgacgac ctcgctgaac
      961 agccacggca ccagcacgtt gccgtcgagc accatcagga tcgcgtaggc cccgatcagc
     1021 cacatgaagt ccggccccca gccccactgg aacagcccga ccagcgccac cggcacggtc
     1081 acgaccgccg cgccgatgta gggcacgatc accgacagcc cgaccatgaa gccgagcagc
     1141 atcgcgtact gcaggccgag ccaggcgaac accaggtagc agatcaccca gttgaccagc
     1201 acctcccaga ccttgccgcg cacgtagttg ccgatctggc ggtcgacatc gtcccagacc
     1261 cggccggcga ggcgggtgtc ggccggcagg aactgccggc accagcgcac gatcggcacc
     1321 ttgtccttca ggaagaagaa caccagcacc ggcaccagga tcaggtagac gatgacggtg
     1381 atgacgctga gcaccgaggc cagcgaccag gtcagcaggt tctggccgac gccggcgatc
     1441 tcgcgcttga ccatcgcgat cgcatcggcg atctgctgct cgctgaccac gcgcgggtac
     1501 tgctcgggca ggctcagcag gtacttctgc gccttcagca gcatgtccgg cagctggcgc
     1561 gcgagctgcg tggcctgctc cgagagcagc ggcagcggcc cggtcagcgc gaaccccacg
     1621 agccccatga agccgaagaa cacgatcagc actgccggcc agcgctgcag ccccgtgcgt
     1681 tcgagcttgg cgaccaggcc ttcgagcagg tacgcgatga cgatgccggc gagcaccggt
     1741 gccagcatcc gcccgagcaa cagcacgatc gcgaccagcg tgacgatgac gatgatcagc
     1801 gacacggcct gagcatcgct gaacacgcgc ctgaaccagg cgaggacgaa attcatgcag
     1861 gctccggtcg accggtcctt cgccggcgga tgatttgaag aatctgggaa aggacggggg
     1921 caggacagaa aaccgggcgg accgcgcagc cggggtggac gatgcagggg ccgctacggc
     1981 tgcagccgca tcggcgcgat gcgggccgac actctaccat ccaacgccat cgagccgctt
     2041 tcacgacgat ccccacggag gtccgtgccg atgaaccgcc cccccgaaac gccgctgccc
     2101 gcccatgccc acgccgagct gctgcagcgc ctgcaggccc tgatcgccta cgtcagccgc
     2161 cgccggcagg tcgagggtga cctgacgcag atcgccgccg acaccgcctc gatgttcggc
     2221 gccgaggcct gctcgatcat gctgttcaag gacggcggca gcgagctgcg cctgcacctg
     2281 accgcgcgct cgcgaccgct gccgaaggaa ctgcgcggcc aggggctcag cgaggcggtc
     2341 ggcgaggggc tggccggccg ggtcgcggcc agcggccggg cgctgctggt ggcggatctc
     2401 gccacgtccg aattcgccgg catcagccgc caccacaccg gcagctgcat ctgtgccccg
     2461 atcccggtcg ccggacgcgt gcgcggcgtg ctgaacctga gccgcccgca gccgttcacg
     2521 cacgacgacc tggcgctcgc ctgcatcgcc ggcctgctgc tcggcctggc gctggagctc
     2581 gcccgcatcc ag
//
LOCUS       DAOQJH010000440         2586 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_28636_length_2586_cov_4.554326, whole genome shotgun sequence.
ACCESSION   DAOQJH010000440 DAOQJH010000000
VERSION     DAOQJH010000440.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2586
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28636_length_2586_cov_4.554326"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(134..209)
                     /locus_tag="PLQ18_09990"
     tRNA            complement(134..209)
                     /locus_tag="PLQ18_09990"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(174..176),aa:Ala,seq:cgc)
     gene            complement(279..878)
                     /gene="amrA"
                     /locus_tag="PLQ18_09995"
     CDS             complement(279..878)
                     /gene="amrA"
                     /locus_tag="PLQ18_09995"
                     /inference="COORDINATES: protein motif:HMM:TIGR00296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HNE02027.1"
                     /translation="MNIEALEHLSLEERAMLLRVARAAIDRGLASRQPPEVEVAAFPP
                     ALREVAATFVTLKLDGELRGCIGTLEARDPLILDVALHAYAAAFDDPRFLPVRRDEFE
                     RLDISVSVLTRPEPLKVSNQAELLEQLRPGRDGLILDYPGHRATFLPAVWKDLPDPLR
                     FVRNLKRKAGLTESFWSPQLRFLRYGSESFDEHDLAPAA"
     gene            complement(868..1692)
                     /gene="amrB"
                     /locus_tag="PLQ18_10000"
     CDS             complement(868..1692)
                     /gene="amrB"
                     /locus_tag="PLQ18_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019643053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HNE02028.1"
                     /translation="MAISPARTLRPPAVAGLFYPEDPVELRASVTAALAGVATAAGAA
                     APKALIVPHAGYVYSGPIAASAYRRISRLHGRIERVVLLGPSHRIGFRGIALTSHAAY
                     VTPLGTVPIDREACALIAALPGVVELDAAHAQEHSLEVHLPFLQLTLGDFRLLPLVVG
                     DADAASVARVLERLWGGAETLVVVSTDLSHYHPYREAQQLDAATSRAIEQLRFESIGP
                     EQACGCRPLGGLLHLAREHGLTVSAIDLRNSGDTAGGRDRVVGYGAYVCHEHPREH"
     gene            1808..>2586
                     /gene="amrS"
                     /locus_tag="PLQ18_10005"
     CDS             1808..>2586
                     /gene="amrS"
                     /locus_tag="PLQ18_10005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013034474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HNE02029.1"
                     /translation="MQRQLFTAPAPARYWHRLDDGRLQCDLCPRFCKLHDGSRGLCFV
                     RGRDGDALVLDTWGRSSGFCIDPIEKKPLNHWLPGTPVLSFGTAGCNLACKFCQNWDI
                     SKSRDTDRLAAQATPAMIAAAAADSGCRSVAYTYNDPVIFLEYAVEVAAACRARGIAS
                     VAVTAGYVCAGPRAEFFAAMDAANVDLKAFTEDFYHRVCGGHLAPVLETLVYLKRETR
                     VWFELTTLLIPGYNDGDDEIARLAEWVGRELGPEVPLHFTA"
BASE COUNT          404 a          859 c          920 g          403 t
ORIGIN      
        1 aaccctcgac cttcgcattg cgaacgcgac gctctcccag ctgagctacc gccccgaaac
       61 ctgttgatcg tctgcccggc cgggttgccg gggtgaaaaa acccgccggc tctggcagcc
      121 ggcgggtggg tgttggtgga gcggaagggg atcgaaccct cgaccttcgc attgcgaacg
      181 cgacgctctc ccagctgagc taccgccccg ttacccttgc gggtgaggcg cgcatcatac
      241 acatcgcccc cccggctcgc cagccctgcg gccgggattc aggccgcggg ggcgaggtcg
      301 tgctcgtcga aggattcgct gccgtagcgc aggaagcgca gctgcggcga ccagaagctc
      361 tcggtcaggc cggccttgcg cttgaggttg cgcacgaagc gcagcgggtc gggcaggtcc
      421 ttccagaccg ccggcaggaa ggtcgcgcgg tggccggggt agtcgaggat caggccgtcg
      481 cggcccgggc gcagttgttc gagcagttcg gcctggttgc tgaccttcag cggctccggg
      541 cgcgtcagca ccgacaccga gatgtcgagc cgctcgaact cgtcgcggcg caccggcagg
      601 aagcgcggat cgtcgaaggc ggcggcgtag gcgtgcagcg cgacatcgag gatcagcgga
      661 tcgcgggctt cgagcgtgcc gatgcagccg cgcagctcgc cgtcgagctt cagcgtcacg
      721 aaggtcgcag cgacctcgcg cagcgccggc gggaaagcgg ccacctcgac ctcgggcggc
      781 tggcgcgagg ccaggccgcg atcgatcgcg gcccgcgcca cacgcagcag catcgcgcgc
      841 tcttcgaggc tcaggtgttc gagggcttca atgttcacgg gggtgttcat ggcagacgta
      901 agcaccgtat ccgacgaccc ggtcgcgtcc gccggcggta tcgccggaat tgcgcagatc
      961 gatggcagac accgtcagac cgtgctcgcg ggcgaggtgc agcagaccgc ccaggggacg
     1021 gcagccgcag gcctgctcgg ggccgatcga ctcgaaccgc agctgttcga tggcgcgcga
     1081 cgtggcggca tcgagttgct gcgcttccct gtaggggtgg tagtggctga gatccgtgct
     1141 caccacgacc agggtttccg cgccgcccca gaggcgttcc agcacgcggg cgacgctggc
     1201 ggcatcggca tcgccgacga ccagcggcag cagccggaag tcgccgagcg tcagctgcag
     1261 gaagggcagg tggacctcca ggctgtgctc ctgcgcatgg gcggcgtcga gttcgacgac
     1321 gccgggcagg gccgcgatca acgcgcaggc ttcgcggtcg atcggaaccg tgccgagcgg
     1381 tgtcacataa gccgcgtgac tcgtcagcgc gatgccgcga aagccgatgc gatgactcgg
     1441 gccgagcagc acgacacgtt cgatgcgtcc gtgcagccgg ctgatccggc gataggcgct
     1501 ggcggcgatc ggtcccgaat agacgtagcc ggcgtgcggc acgatcagcg ccttcggagc
     1561 ggctgcaccg gcggcggtgg cgactccggc gagggccgcc gtgacgctgg cccgcagttc
     1621 caccggatct tccgggtaga acaggccggc aacggccggc gggcgcaggg tgcgggcagg
     1681 gctgatcgcc atcgtctttg tcgcagcgga ctgcggcctc cactcggact ggatcgacac
     1741 tccgtcgaat tctgaacgaa ttctaaatat ttccctgata tgagtcgcag gtacagaggg
     1801 tgttgccatg cagcgacaac tgttcacagc ccccgcaccg gcccgttact ggcaccggct
     1861 cgacgatggc cgcctgcagt gcgacctgtg tccgcgcttc tgcaagctgc acgacggcag
     1921 tcgcggcctc tgcttcgtgc gcgggcgtga cggcgatgcg ctggtgctcg acacctgggg
     1981 gcgctcgtcg ggcttctgca tcgatccgat cgagaagaag ccgctgaacc actggctgcc
     2041 gggcacgccg gtgctgtcct tcggcactgc cggctgcaat ctggcatgca agttctgcca
     2101 gaactgggac atctcgaagt cgcgcgacac cgaccggctc gccgcgcagg cgacgccggc
     2161 gatgatcgcc gcggcggccg cggacagcgg ctgtcgcagc gttgcgtaca cgtacaacga
     2221 cccggtgatc tttctcgaat acgcggtcga ggtcgccgcc gcctgccgcg cgcgcggcat
     2281 cgccagcgtc gcggtgacgg ccggctacgt ctgcgccggg ccgcgcgccg agttcttcgc
     2341 ggcgatggat gcggccaacg tcgatctgaa ggccttcacc gaggatttct atcatcgcgt
     2401 ctgcggcgga catctggcgc cggtgctgga gacgctcgtg tacctgaagc gcgagacccg
     2461 ggtctggttc gagctgacga cgctgctgat ccccggctac aacgacggcg atgacgagat
     2521 cgcgcgcctc gccgagtggg tgggccgcga actcggaccc gaagtgccgc tgcacttcac
     2581 ggcctt
//
LOCUS       DAOQJH010000441         2575 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_28856_length_2575_cov_4.413492, whole genome shotgun sequence.
ACCESSION   DAOQJH010000441 DAOQJH010000000
VERSION     DAOQJH010000441.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2575)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2575)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2575
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28856_length_2575_cov_4.413492"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            14..1060
                     /gene="hflK"
                     /locus_tag="PLQ18_10010"
     CDS             14..1060
                     /gene="hflK"
                     /locus_tag="PLQ18_10010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020160435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HNE02030.1"
                     /translation="MLPGAGAAALLLIWPLAGSFYIVEEGRRGVVVRFGRYVETTMPG
                     LRWHLPYPLERVDIVDVEQVRSEEIGYRSGGRQPASRPVPKEALMLTQDENIVDVRLA
                     VQYQVKDPRAYLFRVADPDTTLRQAVESATREVIGRSSMDFVLTEGRADIVAQIRELA
                     QAVLDQYHTGLRINSVALQDAQPPEEVQEAFGDAIKAREDEQRQKNEAEAYANEIIPK
                     ARGQAARRLQEADAYKAQVIAQAEGEAARFTQLLVQYSKAPQLTRQRLYLETMETVLD
                     ASSKVFIDLDNGGSPLLYLPLDRLLQRSGQPGATGTLLPAPGHDTGNGNPGGAAAGSD
                     PARLRDTGRTREVR"
     gene            1060..1923
                     /gene="hflC"
                     /locus_tag="PLQ18_10015"
     CDS             1060..1923
                     /gene="hflC"
                     /locus_tag="PLQ18_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013819456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HNE02031.1"
                     /translation="MNPRILIAVAGVLAAMLLSASLFTVGEAQKAIRFRLGEIVDTDY
                     PPGLHFKWPLINNVRKFDGRVQTLDTEPERFLTAEKKNVIVDSFVKWRIADTARFYTA
                     VAGDPLQANLRLDQITKDSMRSEFSKRTVQDVVAGDRRQIMDILSTTLRREAANLGVE
                     VVDVRIKRIDLPPDVSSSVFRRMRAERERVARDFRSRGAEAAERIRADADRQSTVLLA
                     EAYRDAERVRGEGDARAAGLYANAYGQDPDFFAFYRSLGAYRASFASKDDVIVLSPDS
                     EFFRYFKKPQP"
     gene            2028..2213
                     /locus_tag="PLQ18_10020"
     CDS             2028..2213
                     /locus_tag="PLQ18_10020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009725853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HNE02032.1"
                     /translation="MWHDLLSAFGLMLVMEGVVPFLSPQALRTALVRLASLSDRELRV
                     GAALSMALGVAVLYWIR"
     gene            2252..>2575
                     /locus_tag="PLQ18_10025"
     CDS             2252..>2575
                     /locus_tag="PLQ18_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HNE02033.1"
                     /translation="MTVKYRWLLPEGIEEILPAGARRVERLRRDLLDLYGRWGYELVM
                     PPFIEFLDALLTGFGRDLELETFKLTDQLSGRLLGVRADMTPQVARIDAHQLARAQPN
                     RLCYLG"
BASE COUNT          395 a          920 c          884 g          376 t
ORIGIN      
        1 cgggcgcggc aagctgctgc ccggtgccgg cgcggcggcg ctgctgctga tctggccgct
       61 ggccggcagt ttctacatcg tcgaggaagg ccggcgcggc gtggtcgtgc gcttcggccg
      121 ctacgtcgag acgacgatgc cgggcctgcg ctggcacctg ccgtatccgc tggagcgcgt
      181 cgacatcgtc gatgtcgagc aggtgcgctc cgaggagatc ggctaccgct ccggcggccg
      241 ccagccggcc tcgcgcccgg tgccgaagga agcactgatg ctgacccagg acgagaacat
      301 cgtcgacgtg cggctggccg tgcagtacca ggtcaaggac ccgcgcgcat acctgttccg
      361 cgtcgccgat cccgacacga cgctgcgtca ggccgtcgag agcgcgacgc gcgaggtcat
      421 cggcaggagt tcgatggatt tcgtgctgac cgagggccgc gccgacatcg tcgcgcagat
      481 ccgcgaactc gcgcaggccg tgctcgatca gtaccacacg ggcctgcgca tcaacagcgt
      541 cgcgctgcag gatgcgcagc cgcccgagga agtccaggag gccttcggcg acgcgatcaa
      601 ggcgcgcgag gacgagcagc gccagaagaa cgaggccgag gcctacgcca acgagatcat
      661 cccgaaggcc cgcggccagg cggcgcgccg gctgcaggag gccgacgcct acaaggcgca
      721 ggtgatcgca caggccgagg gcgaggcagc gcgcttcacg cagctgctcg tgcagtactc
      781 gaaggcgccg caactcacgc gccagcgcct gtacctcgaa accatggaaa ccgtgctcga
      841 cgcgagcagc aaggtgttca tcgacctcga caacggcggc agcccgctgc tgtacctgcc
      901 gctcgatcgc ctgctccagc gctccggcca gcccggcgcc accggcacgc tgctgccggc
      961 cccgggtcac gataccggca acggcaatcc gggcggcgcg gcagccggca gcgatccggc
     1021 acgccttcgc gataccggcc gtacccggga ggtccgctga tgaatccccg catcctgatc
     1081 gccgtcgccg gcgtgctcgc ggcgatgctg ctgtcggcat cgctgttcac ggtcggcgag
     1141 gcccagaagg ccatccgctt ccgcctcggc gagatcgtcg ataccgacta tccgccgggc
     1201 ctgcacttca agtggccgct gatcaacaac gtgcgcaagt tcgacggtcg cgtgcagacg
     1261 ctcgacaccg aacccgagcg cttcctgacc gccgagaaga agaacgtcat cgtcgattcg
     1321 ttcgtgaagt ggcgcatcgc cgataccgcc cgcttctaca ccgcggtcgc cggcgatccg
     1381 ctgcaggcca acctgcgcct cgaccagatc accaaggaca gcatgcgcag cgagttctcg
     1441 aagcgcacgg tgcaggacgt ggtcgccggc gatcgccgcc agatcatgga catcctgtcg
     1501 acgacgctgc ggcgcgaggc ggccaatctc ggcgtcgagg tcgtcgacgt gcgcatcaag
     1561 cgcatcgacc tgccgccgga cgtgtcgagt tcggtgttcc ggcgcatgcg cgccgagcgc
     1621 gagcgcgtcg cgcgtgactt ccgctcgcgc ggcgccgagg ccgccgagcg catccgcgcc
     1681 gatgccgatc gccagagcac ggtgctgctc gccgaggcct accgcgacgc cgagcgcgtg
     1741 cgcggcgagg gcgatgcgcg tgccgccggg ctgtacgcga acgcctacgg ccaggacccg
     1801 gacttcttcg cgttctaccg cagtctcggt gcctaccgcg cgagcttcgc cagcaaggac
     1861 gacgtgatcg tgctgtcgcc cgacagcgag ttcttccgct acttcaagaa gccgcagccc
     1921 tgaggctgcg ggcgcgtcgc gtcgccacgg gcgggccctc gcaaggaggc ccgcccgttg
     1981 cattgcggcc gcccgcgcgc aggggccgcg cgccagcggt gtgcatgatg tggcatgatc
     2041 tgctgtctgc cttcggcctg atgctggtga tggagggcgt cgtgcccttc ctgagtccgc
     2101 aggcgctgcg cacggcgctg gtgcggctcg cgagcctcag tgaccgcgag ctgcgcgtcg
     2161 gcgccgcact gagcatggcg ctcggcgtcg ccgtgctgta ctggatccgc tgagcgcgcg
     2221 agcgcgcccg gcccgaccgg aagtttcatg catgaccgtc aaataccgct ggctgctgcc
     2281 ggagggcatc gaggaaatcc tgcccgccgg tgcgcgccgc gtggagcgcc tgcgccgcga
     2341 cctgctcgac ctgtacggac gctggggcta cgaactcgtg atgccgccgt tcatcgagtt
     2401 cctcgatgcg ctgctgaccg gcttcggccg tgacctcgaa ctcgaaacct tcaagctgac
     2461 cgaccagctc tccgggcggc tgctcggcgt gcgcgccgac atgacgccgc aggtcgcgcg
     2521 catcgacgcg caccagctcg cgcgggcgca gccgaaccgc ctctgctatc tcggc
//
LOCUS       DAOQJH010000442         2570 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_28953_length_2570_cov_4.513320, whole genome shotgun sequence.
ACCESSION   DAOQJH010000442 DAOQJH010000000
VERSION     DAOQJH010000442.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2570
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28953_length_2570_cov_4.513320"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..260
                     /locus_tag="PLQ18_10030"
     CDS             <1..260
                     /locus_tag="PLQ18_10030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02034.1"
                     /translation="PCGESGRIFRKSGLAAVYVRGQADSGRAGRAEWEMRAGGTTGSI
                     RWQDAQSLCARRAPDGAGINRCEAMSIWREAIGPFMATTRY"
     gene            complement(307..942)
                     /locus_tag="PLQ18_10035"
     CDS             complement(307..942)
                     /locus_tag="PLQ18_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009617717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HNE02035.1"
                     /translation="MEHTGLFIGALVTVYLLPGPDMLLILQTAGTQGRRAALAVAAGL
                     ALARCIHVTLAGLGLAALLHNSPQTFEGLRLVGAAYLVWLGLHIARSPLPCAQDPARE
                     PPMPAHSLAAAALRGLLTNLTNPKALLFCSMLLPQFVSVDATDPAGPFFRLGAVLVGI
                     GLLFDGLYVFAGIVIGHWLRRSVLRRRMQQWGFGALLVGFGLRLALVGRMD"
     gene            1075..1566
                     /locus_tag="PLQ18_10040"
     CDS             1075..1566
                     /locus_tag="PLQ18_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014996537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HNE02036.1"
                     /translation="MPILKPDRLDRRILEALQRDARLSNVELAEAIGLSPSPCLRRVR
                     LLEEAGVISGYHAALDRNAVGLGLTVFVGVKVGPHHEEASTAFREAVVQLPEVVSCHL
                     VSGEADFLLQVVVPDLPAYERFLLGTLLKLPGVSDIRSNFAIATVKAQTALPLAHLGA
                     GGG"
     gene            1731..>2570
                     /locus_tag="PLQ18_10045"
     CDS             1731..>2570
                     /locus_tag="PLQ18_10045"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNE02037.1"
                     /translation="MHDIAIVGGGLCGLALLRLLSGSGLDVVLFEARARLGGRVLSVP
                     ARGGHPGGDLGPGWYWPDTQPLLNGLITELGLRTFAQHDQGISLYVGGPDQPPASLGE
                     RPVHDGARRIDGGMGRIIEGLAHALPPARLQTGCRLHRLRDHGDHVELGFTDTAFGRT
                     VRARHVVLALPPRLIDETLAFEPALPAELRAAMRDTPTWMAAQAKLLAACEQPVWRVF
                     GCSGNVGNVPPKAATIGPRDVLHAKKRVECHGLTTMGTFSSWQRMTSRLITHGPVTPL
                     VPAS"
BASE COUNT          400 a          872 c          896 g          402 t
ORIGIN      
        1 gcccatgtgg tgagtccgga agaattttcc gaaaatcagg attggctgcc gtgtatgtgc
       61 gcggacaggc tgattccgga agagcgggca gggcggaatg ggagatgcgt gcaggtggta
      121 cgacgggcag cattcgatgg caggatgccc agtcgctttg tgcccgccgt gcgccggatg
      181 gcgccggcat caaccgctgt gaggcaatga gcatctggcg tgaagccatc gggccgttca
      241 tggcgacgac ccggtattga tcatcgcaag ctgtcgttct cccttgcggc cacggcaccg
      301 gcccgctcag tccatccgcc cgaccagcgc cagcctcagc ccgaagccca ccagcagcgc
      361 cccgaatccc cactgctgca ttcgccggcg cagcacgctg cgtcgcagcc agtggccgat
      421 gacaatgccg gcaaagacat acagcccatc gaacagcaga ccgatgccga ccagtacggc
      481 accgagccgg aagaacgggc cggccgggtc ggtggcatcg accgatacga actgcggcag
      541 cagcatcgag cagaacagca gggccttggg attggtgaga ttggtcagca gcccgcgcaa
      601 tgcggcagcg gccagactgt gcgccggcat cggcggctcg cgggccgggt cctgtgcgca
      661 gggcagcgga ctgcgtgcga tgtgcaggcc gagccagacc agatacgccg cgccgaccag
      721 gcgcagcccc tcgaaggtct gcggcgagtt gtgcagcagg gccgccaggc cgagtccggc
      781 cagcgtcacg tgaatgcagc gcgccagcgc cagccctgcg gccaccgcca gcgcagcccg
      841 gcggccctgc gtaccggcgg tctgcaggat cagcagcatg tccggaccgg gcagcagata
      901 gaccgtgacc agcgcaccga tgaacagccc cgtgtgttcc atgaccgggc ctcccgtctg
      961 tggaatcgag cgggcagggt agacgcatga ccgaagccgg ggattgcgta ttcagccatc
     1021 catgcctacg gttctggcag aacatgccat tgattgatct ttaatctgtt gatcatgcca
     1081 atcctgaaac ccgaccggct cgaccggcgc attctcgaag ccttgcagcg cgatgcgcgc
     1141 ctgagcaacg tcgaacttgc cgaggccatc ggtctgtcgc cgtcgccctg cctgcggcgc
     1201 gtgcgcctgc tggaagaggc cggcgtcatc agcggctatc acgcggcgct ggatcgcaac
     1261 gcggtcgggc tggggctgac ggtgttcgtc ggcgtcaagg tcggtccgca ccatgaagaa
     1321 gcctcgacgg cctttcgcga ggccgtcgtg caactgcccg aggtcgtctc ctgccatctg
     1381 gtttccggcg aggccgactt cctgctgcag gtggtcgtgc ccgatctgcc ggcctatgaa
     1441 cgcttcctgc tcggcacgct gctgaagctg cccggcgtca gcgacatccg cagcaacttc
     1501 gcgatcgcca cggtcaaggc gcagacggcg ctgccgctcg cgcatctggg ggcgggcggc
     1561 ggctgacgcg gcagcgatcc gacaattccg acggttcctg tcggattccc taccgccccg
     1621 ctcgggctgc catgacgtgc aagtgcctga accggcggtt gcctgtgtca atttgcatgg
     1681 aacttgctcc tgtgccccgc gcagattcgg gttcggggaa gggctgggcc atgcatgaca
     1741 ttgcgatcgt cggtggcggg ctgtgcgggc tggcgctgct gcggctgctc agcggcagcg
     1801 ggctcgatgt ggtgctgttc gaggcgcggg cgcggcttgg cgggcgggtg ctgtcggtgc
     1861 cggcgcgcgg cgggcatccg ggcggcgatc tcgggccggg ctggtactgg ccggacacgc
     1921 agccgctgct gaacggcctg atcaccgaac tggggctgcg caccttcgcg cagcacgatc
     1981 agggcatctc cctgtacgtc ggcgggccgg accagccgcc ggcgagcctc ggcgagcggc
     2041 cggtgcatga cggtgcgcgg cgcatcgacg gcggcatggg ccgcatcatc gaggggcttg
     2101 cgcacgcgct gccgcccgcc cgcctgcaga ccggctgccg gctgcaccgg ctgcgtgacc
     2161 acggcgatca cgtcgaactc ggtttcaccg acacggcctt cggacgcacc gtgcgcgccc
     2221 ggcacgtcgt gctggcgctg ccgccgcggc tgatcgacga gacgctcgcg ttcgagccgg
     2281 ccctgccggc ggagctgcgc gcggcgatgc gcgacacgcc gacctggatg gcggcgcagg
     2341 ccaagctgct cgccgcctgc gagcagccgg tctggcgcgt gttcggctgc tccggcaacg
     2401 tgggcaacgt gccgcccaag gccgccacca tcggcccgcg cgacgtgctc cacgcgaaaa
     2461 agagggtcga gtgtcatggc ctgaccacca tgggcacatt ctcctcctgg cagcgcatga
     2521 cctcgcgcct gatcacgcac ggcccggtca cgcccctggt accggcctcc
//
LOCUS       DAOQJH010000443         2554 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_29269_length_2554_cov_2.523009, whole genome shotgun sequence.
ACCESSION   DAOQJH010000443 DAOQJH010000000
VERSION     DAOQJH010000443.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2554
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29269_length_2554_cov_2.523009"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            109..495
                     /locus_tag="PLQ18_10050"
     CDS             109..495
                     /locus_tag="PLQ18_10050"
                     /inference="COORDINATES: protein motif:HMM:NF022795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3TM-type holin"
                     /protein_id="HNE02038.1"
                     /translation="MALDPVSAGLDLVNSIVTRVWPDASEADRARAATALTALQGQLA
                     VNEAEARSPSVWTSGWRPGVGWVCVAAFAWRYVGYPVVSLGCALWAPGVHLPDPGIDP
                     ALTELLIGMLGLGGLRTVEKMKGVAR"
     gene            492..767
                     /locus_tag="PLQ18_10055"
     CDS             492..767
                     /locus_tag="PLQ18_10055"
                     /inference="COORDINATES: protein motif:HMM:NF013427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HNE02039.1"
                     /translation="MKPFEHEPAADVLDVAAERAQIELDAALADRRCRNDRLFRGTAD
                     PDRVCEECGAPIGARRLELVPGATRCVDCQEIAERCAASAGLERIAG"
     gene            764..1486
                     /locus_tag="PLQ18_10060"
     CDS             764..1486
                     /locus_tag="PLQ18_10060"
                     /inference="COORDINATES: protein motif:HMM:NF022432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2786 domain-containing protein"
                     /protein_id="HNE02040.1"
                     /translation="MNRADVIRKIRACLALSASPEPAEAAAALRQAEKLMREHAVELA
                     DVHASETTQELAKSGFKTDPPAYAVILSGMVARAFQCDRIGMPGRTLTRTETSETYVP
                     ALWVFIGIAPRPELAVYAYEVLLRQLQSARRRYVRNLDKRCKPKTREARSEAFTVGWL
                     NAVQQEVNRFAGRSEDDERAVLAFLRSHYPNLRTSKTELKASARHAAAVHDGLQAGRA
                     AQLRHGLNEAPPRPALTGGSGS"
     gene            1483..1998
                     /locus_tag="PLQ18_10065"
     CDS             1483..1998
                     /locus_tag="PLQ18_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005488851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HNE02041.1"
                     /translation="MNIAWGHSVSPEFKTRVVELATELGCDPSHLMACMAFESGGTFS
                     PSVRNAAGSGAVGLIQFMPATAAGLGTSTDALARMTPLAQLEYVRRHFLPFKGRLATL
                     EDVYMTILWPAAVGRPAASVLFARDSDRPKVYFQNRGLDLNKDGSITKAEAAAKVQAA
                     LVLGMKAGNIG"
     gene            2014..>2554
                     /locus_tag="PLQ18_10070"
     CDS             2014..>2554
                     /locus_tag="PLQ18_10070"
                     /inference="COORDINATES: protein motif:HMM:TIGR01641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor head protein"
                     /protein_id="HNE02042.1"
                     /translation="MAEPQLGAVPFAEAIAHLRQKVRLPTARSDDLHGRVHAKAFTVA
                     GAQKAGLLADLHEAIVAARAGGQSIGQFRKAFDEIVTRHGWSYHGKRGWRTRVIYDTN
                     MATSAMAGQWQQFQSTAGTRPYLQYQTVGDGRVRPQHRAWHGTVRPIGDGFWDSHYPP
                     NGWGCRCTVRSLSDVQMQRD"
BASE COUNT          417 a          834 c          923 g          380 t
ORIGIN      
        1 aatacgaagt gcatcgctgg ctcgagatag gcaagggcca cctgcagcag ggcttcatgg
       61 cgctgacgcg ggccgtcacc agaccgaccg ggttctgagg gcgaggagat ggcactggac
      121 cccgtgtcgg ccgggctcga cctggtcaac agcatcgtca cccgcgtctg gcccgatgct
      181 tccgaggccg accgcgcccg ggccgcgact gcgctgacgg ccctgcaggg ccagctcgcg
      241 gtcaacgagg ccgaggcgcg cagcccctcg gtctggacct ccggctggcg gcccggcgtc
      301 ggctgggtct gcgtcgctgc gttcgcctgg cgctacgtcg gttatccggt cgtctcgctc
      361 ggctgcgcac tgtgggcgcc gggcgtgcac ctgccggacc ccggcatcga tccggcactg
      421 accgagttgc tgatcggcat gctcgggctc ggcgggctgc gtacggtcga gaagatgaag
      481 ggagtcgcac gatgaagccg ttcgaacatg aacccgcggc cgatgtgctc gacgtggcgg
      541 ccgagcgcgc gcagatcgaa ctggacgcag cgctggccga ccgccggtgc aggaacgacc
      601 ggctgttccg cggtaccgct gatccggatc gggtgtgcga ggagtgcggc gcgccgatcg
      661 gcgcccgccg cctggagctt gtacccgggg ccacgcgctg cgtggactgt caggagatcg
      721 ccgagcgctg cgccgccagc gccggactgg agaggatcgc cggatgaatc gcgctgacgt
      781 gattcgcaag attcgtgcgt gcctggcgct ctccgccagt ccggagccgg ccgaagccgc
      841 tgcagcgctg cgtcaggccg aaaaactgat gcgcgagcat gcggtcgagc tggccgacgt
      901 gcatgcgtcc gagacgacgc aggagcttgc gaaatccggc ttcaagaccg acccgccggc
      961 gtacgccgtg attctctccg gcatggtcgc ccgcgcgttc cagtgcgacc ggatcggcat
     1021 gcccggtcgt acgctcacgc gcaccgagac cagcgagacc tacgttcctg cgctatgggt
     1081 tttcatcggg atagcgccgc ggcccgaact ggcggtctac gcctacgagg tgctgctgcg
     1141 ccagctccag tccgcgcgcc ggcgctacgt tcgcaacctc gacaaacgct gcaagccgaa
     1201 gactcgcgag gcgcgatccg aggcattcac ggtcggctgg ctgaacgcgg tgcagcagga
     1261 agtgaatcgc ttcgccgggc gtagcgagga cgacgaacgc gcggtgctgg ccttcttgcg
     1321 aagtcactac ccgaatctgc gcaccagcaa gaccgagctc aaggccagtg cacgccacgc
     1381 tgcagcggtg cacgacggtc tgcaggccgg ccgggcggcg cagttacggc atgggctcaa
     1441 cgaagcgccg ccgcggccgg cgctgacggg aggaagcgga tcatgaacat cgcatggggc
     1501 cattcggttt caccggaatt caagacacgc gtggtcgagc tcgccacgga gctgggctgc
     1561 gacccgagcc acctcatggc ctgtatggca ttcgagtccg gcggcacgtt cagcccgagc
     1621 gtgcgcaacg ctgctggcag cggtgcggtc gggctgatcc agttcatgcc agcgacggcc
     1681 gcggggctcg gcacgagcac cgacgcgctg gcgcgcatga cgccgctcgc gcagctcgaa
     1741 tacgtgcgcc gacacttcct gccgttcaaa ggccggctgg cgacgctgga ggatgtctac
     1801 atgacgatcc tctggccggc tgcggtcggc cggccggcag cgtcggtgct gttcgcacgc
     1861 gacagcgatc gaccgaaggt gtacttccag aatcgcgggc tggacctgaa caaggacggc
     1921 tcgatcacca aggccgaggc cgcagcgaag gtgcaggcgg cgctggtgct cggcatgaag
     1981 gcggggaaca tcgggtgatc gaggtcgcac gacgtggctg agccacagct cggggcggtg
     2041 ccgttcgcgg aggcgattgc gcatctgcgg caaaaggtga ggctgccgac cgcgcgcagc
     2101 gacgatttgc acgggcgcgt gcatgcgaag gcgttcacgg ttgccggtgc gcagaaggcc
     2161 ggcctgctcg ccgatctgca cgaggcgatc gtcgcggcgc gcgccggcgg gcagagcatc
     2221 gggcagttcc gcaaggcgtt tgatgagatc gtcacccggc acggctggag ctaccacggc
     2281 aaacgcggct ggcggaccag agtcatctac gacacgaaca tggccacttc ggcgatggcc
     2341 gggcagtggc agcagttcca gagcaccgcg ggcacacggc cttacctgca gtaccagacg
     2401 gtcggtgatg gccgtgtgcg acctcagcat cgggcgtggc acgggaccgt gcggccgatc
     2461 ggcgacgggt tctgggacag ccactatccg ccgaacggct ggggctgccg gtgcaccgtg
     2521 cgctccctct cggatgtgca gatgcagcgc gacg
//
LOCUS       DAOQJH010000444         2548 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_29377_length_2548_cov_3.790213, whole genome shotgun sequence.
ACCESSION   DAOQJH010000444 DAOQJH010000000
VERSION     DAOQJH010000444.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2548)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2548)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2548
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29377_length_2548_cov_3.790213"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..301
                     /locus_tag="PLQ18_10075"
     CDS             <1..301
                     /locus_tag="PLQ18_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HNE02043.1"
                     /translation="LPADTILNVNVPAVPWSELRGWQVTRLGHRHKAEPAVCTRDPRG
                     RPIWWIGPAGAESDAGPGTDFQAIREHCVSVTPIQVDLTRHAALDTVARWLDAPA"
     gene            369..1013
                     /locus_tag="PLQ18_10080"
     CDS             369..1013
                     /locus_tag="PLQ18_10080"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007155462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HNE02044.1"
                     /translation="MTSARTRTRLVARLREQGIDDERVLAVMRETPRHLFIEEALASR
                     AYEDTALPIGLGQTISQPYIVARMTQALLAGREALPLRVLEIGTGSGYQSAVLAPLVG
                     QVYSVERIERLSLRARERLAALGLRNVHLKHADGSIGWPGCGPYDGILVTAAAERLPP
                     ALPEQLATGGRLVAPLGPAGGVQELLIVERKAIGYETHSLGAVVFVPLLAGVQP"
     gene            1038..1829
                     /locus_tag="PLQ18_10085"
     CDS             1038..1829
                     /locus_tag="PLQ18_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008931303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HNE02045.1"
                     /translation="MPFLLHRYRAPVTRALAALLLAGCAAYGPERPSEYVVVRGDTLY
                     AIAWRFGIERNDLVRWNGITNPDRILVGQRLVLAPPETGAPAMRNAGPVVVAAEARDS
                     PRSTPVRSARVPRRTTAGVAAARPVAARASADDGDDSLARGPWRWPVRGELLRRYDAD
                     GPGRKGIEIGAGLGQTVSAAQGGEVVYSGSGLPGYGRLIIIKHSESMLSAYGFLGKIL
                     LNEGDKVRAGQAIGEVGTSSENRPALHFEIRQNGRPVDPLRYLPG"
     gene            1979..>2548
                     /locus_tag="PLQ18_10090"
     CDS             1979..>2548
                     /locus_tag="PLQ18_10090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020411739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNE02046.1"
                     /translation="MPRRTPDLGGFTEEPEAFAVVEEEEETLLDTDEAGPDADDVAAE
                     ALVEAGLDEAEPVEDDERTLSKTPLYGKDFSQDELDATRLYLGEIGFSPLLTAQEEVY
                     FARRALRGDEAARKRMIESNLRLVVKISRRYMNRGLTLLDLIEEGNLGLIHAVEKFDP
                     ERGFRFSTYATWWIRQTIERAIMNQTRTIR"
BASE COUNT          402 a          863 c          878 g          405 t
ORIGIN      
        1 gctgccggcc gacaccatcc tgaacgtcaa cgtgccggcc gtgccgtgga gcgagctgcg
       61 cggctggcag gtcacgcggc tcggtcaccg gcacaaggcc gagccggcgg tctgcacccg
      121 cgatccgcgc ggccggccga tctggtggat cggtccggcc ggcgccgaga gcgacgccgg
      181 acccggtacc gacttccagg cgatccgcga gcactgcgtg tcggtgacgc cgatccaggt
      241 cgacctgacg cgccatgccg cgctcgatac ggtcgcacgc tggctggatg cgccggcttg
      301 atcccggcca tggccgctcg cccgtggacg cagccgtgag ccgcccggac gccttccgcg
      361 gcatcggcat gacctcggcc cgcacccgca cccggctggt cgcgcggctg cgcgagcagg
      421 gcatcgacga tgagcgcgtg ctggcggtga tgcgggagac gccgcgccac ctgttcatcg
      481 aggaggcgct ggccagccgc gcctatgagg acaccgcgct gccgatcggc ctcggccaga
      541 ccatctcgca gccgtacatc gtcgcgcgca tgacgcaggc gctgctcgcc gggcgcgagg
      601 cgctgccgct gcgggtgctg gagatcggca ccggttcggg ttatcagagt gccgtgctcg
      661 ccccgctggt cgggcaggtc tacagcgtcg aacgcatcga gcgcctgtcg ctgcgcgcgc
      721 gtgagcggct cgcggcgctg gggctgcgca acgtgcatct gaaacacgcc gacggcagca
      781 tcggctggcc cggatgcggt ccgtatgacg gcattctggt caccgcggcg gccgaacggc
      841 tgccgccggc gctgccggag cagctcgcga ccggcgggcg gctggtggcg ccgctcggtc
      901 cggccggcgg cgtgcaggaa ctgctgatcg tcgagcgcaa ggcgatcggc tacgaaaccc
      961 attcacttgg cgccgtcgtg ttcgtgccgc tgctcgccgg cgtccagccc tgaaccccca
     1021 gcctgccgga catgcccatg ccgttcttgc ttcaccgcta tcgtgcgccc gtgacgcggg
     1081 cgctcgccgc cctgctgctc gccggctgtg cggcctacgg tcccgagcga ccgtccgaat
     1141 acgtggtcgt gcgcggcgac acgctgtatg cgattgcctg gcgcttcggc atcgaacgca
     1201 atgatctggt gcgctggaac ggcatcacca atcccgaccg catcctggtc ggccagcggc
     1261 tcgtgctcgc cccgcccgag accggcgcgc cggcgatgcg caacgccggt ccggtggtcg
     1321 tcgctgccga agcccgggac agtccgcgca gcacaccggt gcgcagcgcg cgcgtgccgc
     1381 ggcgcacgac agccggcgtc gccgcggcac ggccggtggc tgcacgcgca tcggccgatg
     1441 acggcgatga ttcgctcgcg cgcggcccct ggcgctggcc ggtgcgcggc gagctgctcc
     1501 gccgttacga tgccgacggc cccggccgca agggcatcga gatcggtgcc ggtctcggcc
     1561 agaccgtgag cgccgcgcag ggcggggagg tcgtctatag cggcagtgga cttcccggat
     1621 atggccgact tatcatcatc aagcattccg aatccatgct cagcgcctat ggatttctcg
     1681 gaaaaatcct tctgaatgag ggtgataagg tccgcgccgg tcaggcgatc ggcgaggtcg
     1741 gcaccagcag cgagaatcgt ccggcgctgc atttcgaaat ccgccagaac ggccggccgg
     1801 tcgatcccct ccgctatttg ccgggttgaa cttcctgacc gtccaccccg acaatcgggt
     1861 cggggggcgg cggattgcgc ctcgatgacg caaggccggc tgaagcctgc gcagataaag
     1921 attaagaaaa ggacatattc cccaccacca gagccgagca gggtgtcagg aggtttccat
     1981 gccgcgtcgt actcccgatc tgggcggatt taccgaagaa cccgaagctt ttgcggtcgt
     2041 cgaggaggaa gaggaaacct tgctcgatac cgatgaagcc ggtcccgatg ctgatgacgt
     2101 tgcggccgaa gcgctggtcg aggccggtct ggatgaggcc gagccggtcg aggatgacga
     2161 gcgcacgctc agcaagacgc cgctgtacgg caaggatttc agccaggacg aactcgatgc
     2221 cacgcggctg tatctcggcg agatcggctt ttcaccgctg ctgaccgcgc aggaggaagt
     2281 ctatttcgcg cgccgggcat tgcgtggcga tgaagccgca cgcaagcgca tgatcgaaag
     2341 caatctgcgt ctggtcgtga agatttctcg ccgttacatg aatcgcggcc tgacgctgct
     2401 cgacctgatc gaggaaggca atctcggcct gattcacgcc gtcgagaaat tcgatcccga
     2461 gcgcggattc cggttttcga cctatgcgac ctggtggatt cgccagacca tcgagcgcgc
     2521 gatcatgaac cagacgcgca cgatccgg
//
LOCUS       DAOQJH010000445         2547 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_29398_length_2547_cov_3.248796, whole genome shotgun sequence.
ACCESSION   DAOQJH010000445 DAOQJH010000000
VERSION     DAOQJH010000445.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2547
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29398_length_2547_cov_3.248796"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1085
                     /locus_tag="PLQ18_10095"
     CDS             <1..1085
                     /locus_tag="PLQ18_10095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HNE02047.1"
                     /translation="WIRNTVGDACAAHALLAAAMAGRGQPAESLTLFHARFTVADRQR
                     IETDVVARFGRNGDPAGRRGRIVIGSQVLEQSLDVDFDVLVSDLAPIDALIQRAGRMC
                     RHRRDASGAVRTDDDPRPDARVPCLWVHGPAPVADAGADWYRAMFRGAAAVYPNHRQL
                     WLTAAELERRGGFAMPDDARALIETVFGDDAEAPAALDRSDINTDGERHAATAMAQFN
                     ALQLDAGYQRSGSQWADDSVTPTRLGDAETVLRLAVYVDGDWRPLHDEAQGWERSQVR
                     LRQRHARRRAPLSDPMLEAAAVSAESRLPDRGKWSVLLPLFPDGEACWRATVLDANDQ
                     PCTLWYGPRFGLLREQEQNVSKESCP"
     gene            1082..>2547
                     /gene="casA"
                     /locus_tag="PLQ18_10100"
     CDS             1082..>2547
                     /gene="casA"
                     /locus_tag="PLQ18_10100"
                     /inference="COORDINATES: protein motif:HMM:NF021030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
                     /protein_id="HNE02048.1"
                     /translation="MNLIDDTWIPVRRHSGIRALIAPWQIAESDDPVIAIDSGRPDFD
                     DALFQFLIGLLQTLCAPTDELDWADRLDPPAVERLRERFATVADAFGLDGDGPRFMQD
                     FDAKLGPAENDIDSLLIEAPGGQTKRNNADHFIKGGLVGGICPACAAQALLTLNTNAP
                     AGGAGIRTSLRGGGPLTTLLRFDPQARRDGQPVMLWHDLWLNVLTRQRFFGHWHGADD
                     ARAWFPWLSPTRTSEKNEQITAADAHPAVAFWATPRRIRLDWSSTTAGHCDLCGAESE
                     RLVQRYASRPRGANYTTWLHPLSPYYRLKATSTEWLPQHPQPGGIGYRHWPELLFGDE
                     ASTRRPAAVIVEKTDRVLGEVDPQRWNTGLPLQLWAGGYDMDNMKARCWYEARMPVFT
                     SGKPEVRKELRHLSAQFVEAAGLVRACLGSAVRAAWFARPADAKGDFGFLDRAFWDAT
                     ETPFFRLVQTLSGIDLTRDASDALRLQARESWLQTLRTT"
BASE COUNT          411 a          858 c          860 g          418 t
ORIGIN      
        1 gctggatccg caataccgtc ggcgatgcct gtgccgcgca tgcgctgctc gcggcggcaa
       61 tggccggacg cggccagccg gccgagtcgc tgacgctgtt ccacgcccgc ttcacggtcg
      121 ccgatcgcca gcgcatcgag accgatgtcg tcgcccgctt cgggcgcaac ggtgacccgg
      181 cgggtcggcg cggccggatc gtgatcggct cccaggttct ggagcagtcg ctggatgtgg
      241 atttcgacgt gctggtctcc gacctcgcac cgatcgacgc gctgatccag cgcgcagggc
      301 gcatgtgccg gcaccggcgc gatgcaagcg gtgccgtgcg caccgacgac gatccccggc
      361 cggatgcccg tgtgccctgc ctgtgggtgc atggtccggc gccggtcgct gatgccggag
      421 ccgactggta tcgcgcgatg ttccggggcg ctgcagcggt atatccgaac catcgccagc
      481 tctggctgac cgccgccgaa ctcgaacgcc gcggcggttt cgcgatgccg gacgacgcgc
      541 gggcgctgat cgagaccgta ttcggcgatg acgccgaagc acccgccgca ctcgatcgca
      601 gcgacatcaa cactgacgga gagcggcacg cggcaacggc catggcgcaa ttcaacgcgc
      661 tgcagctcga tgcgggttac cagcgcagcg gcagccagtg ggccgacgac agcgtgaccc
      721 cgacccggct cggcgacgcc gaaaccgtgc tgcggctggc cgtgtacgtc gatggcgact
      781 ggcgtccgct gcatgacgag gctcagggct gggaacgctc gcaggtaagg ctgcgccagc
      841 gccacgccag acgccgcgcc ccgctcagcg acccgatgct cgaagccgcg gcggtatccg
      901 ctgaatcccg gctgccggac cgcggcaaat ggagcgtgct gctgccgctg ttcccggacg
      961 gcgaagcgtg ctggcgcgcc acggtgctgg atgccaacga tcagccctgc acgctctggt
     1021 acgggccgcg cttcgggttg ttgcgtgaac aggaacaaaa cgtcagcaag gaatcctgcc
     1081 catgaatctg atcgacgaca cctggattcc cgtccgccgc cacagcggca tccgggcact
     1141 gattgcaccg tggcagattg ccgaatccga tgatccggtc attgccatcg attccggccg
     1201 gccggatttc gatgatgcac tgtttcagtt cctgatcggc ctgctgcaga ccttgtgcgc
     1261 tccgactgac gaactcgact gggcagaccg gctggatcca ccggctgtcg agcggttgcg
     1321 cgagcgtttc gccacggttg ccgatgcgtt tgggctggac ggcgacgggc cgcggttcat
     1381 gcaggatttc gatgcaaagc tcggtccggc ggaaaacgat atcgacagcc tgctgatcga
     1441 ggcgccgggc ggacagacca aacgcaataa cgccgatcat ttcatcaagg gcgggctggt
     1501 cggcgggatt tgcccggcct gtgccgcgca ggcgctgctg acgctgaata ccaatgcgcc
     1561 ggccggcggt gccggcattc gcacttcgct gcgcggcggc gggccgctga cgacgctgct
     1621 gcggttcgat ccgcaggcgc gccgtgacgg tcagccggtg atgctctggc atgatctctg
     1681 gctcaacgtg ctgacccgcc aacgcttctt cggtcattgg cacggtgccg acgatgcccg
     1741 cgcctggttt ccgtggctgt cgccgacgcg caccagcgaa aagaacgagc agatcaccgc
     1801 ggccgatgcc catccggccg ttgccttctg ggccacgccg cgacgcatcc ggctcgactg
     1861 gagcagcacc acggcgggcc actgcgatct ctgcggtgcc gaatccgaac gactcgtcca
     1921 gcgttacgcc agtcgcccgc gcggcgcgaa ctacaccacc tggctgcatc cgctgtcgcc
     1981 gtattaccgg ctgaaagcga cttcgaccga atggctgccg cagcatccgc aacccggagg
     2041 catcggttac cggcactggc cggaactgct gttcggcgac gaggcctcga cccggcggcc
     2101 ggcggccgtg atcgtcgaga aaaccgatcg cgtactcggc gaagtcgatc cgcaacgctg
     2161 gaataccggc ctgccgttgc agctatgggc cggcggttac gacatggaca acatgaaggc
     2221 gcgctgctgg tacgaggcca ggatgccggt attcacctcg ggcaagccgg aggttcgcaa
     2281 ggaactgcgg catctgtccg cgcaattcgt cgaggccgcg ggactggtac gcgcctgtct
     2341 gggcagtgcg gtacgcgccg cctggtttgc acgccccgcc gatgcgaaag gggatttcgg
     2401 ttttctcgac cgcgcgttct gggatgcgac cgaaacgccg ttcttcaggc tggtgcagac
     2461 cttgtccggg atcgatctga cccgggacgc cagcgacgcc ctgcggctgc aggcacgcga
     2521 atcctggctg cagaccctgc gcacgac
//
LOCUS       DAOQJH010000446         2546 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_29408_length_2546_cov_5.302288, whole genome shotgun sequence.
ACCESSION   DAOQJH010000446 DAOQJH010000000
VERSION     DAOQJH010000446.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2546)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2546)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2546
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29408_length_2546_cov_5.302288"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..599)
                     /gene="aceE"
                     /locus_tag="PLQ18_10105"
     CDS             complement(<1..599)
                     /gene="aceE"
                     /locus_tag="PLQ18_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006478378.1"
                     /note="E1 component; part of pyruvate dehydrogenase; forms
                     a complex with DlaT and LpdC; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HNE02049.1"
                     /translation="MTAEQYKDVDPQETREWLAALDGVIAHVGAERAHWLIEELIERT
                     RLAGDLAPFSANTPYVNTVPVEKQAPIPGDQDIEHRIRSFVRWNAAMMVLRANRETNV
                     GGHIASFASAATLYEVGYNHFWRAPSDGHNGDLLFIQGHSSPGIYSRAFLLGRLSAEQ
                     MDNYRQETDGKGLSSYPHPWLMPDFWQFPTVSMGLGPLLA"
     gene            994..>2546
                     /locus_tag="PLQ18_10110"
     CDS             994..>2546
                     /locus_tag="PLQ18_10110"
                     /inference="COORDINATES: protein motif:HMM:NF026177.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HNE02050.1"
                     /translation="MTLRRLRIGTRLALGFGAILVIVAATLTAAMLHDLAGIQTASRA
                     ERLNGSIGLVIASMQREQRAAEALATLVASQPSVVEAFAAGDRERLLAEFGGAFAALK
                     TGQNLEQFQFHGPGAISFLRVHQPQQHGDDLSAARRTIVDAQQSGKAVAGLEADAGGF
                     GLRAVVPIRRGEQLLGTVEFGVAMGQGFFDELMRWYLLRARLVTLDDAGQLRRLADTA
                     GNWPDLGEAELRAAAKGTPAMGDIITGDAHLAVLIMPIADYAGRTAGALEVFQDQTLF
                     EQTARQIRQTALALGAGLLLLGCLLAWWLARGIARPLGAMAEAAQAIAAGDTSCEVRY
                     VGRDEVGRLADAFRAMLAYLRELTQAVDALDRGDTARRHTARSEADRLGTSVARTQAT
                     VAELLAETQSLVHAAEVGELARRGRTERFSGDYGALVASVNHLLDTRAAARERETAAE
                     AERLAAARAATEQLATQVETLLARVDAAARGDLTVQVDGSDEDAIGRVGAGLSRLLGD
                     LRGHLGAIG"
BASE COUNT          368 a          912 c          881 g          385 t
ORIGIN      
        1 gcgagcagcg ggccgaggcc catcgacacg gtcgggaact gccagaagtc cggcatcagc
       61 cacggatgcg ggtaagagct caagcccttg ccgtcggtct cctggcggta gttgtccatc
      121 tgctcggcgc tcaggcgccc gagcaggaag gcgcgcgagt agatgccggg ggacgagtga
      181 ccctggatga acagcaggtc accgttgtgg ccgtcgctcg gcgcacgcca gaagtggttg
      241 tagccgacct cgtagagggt cgcggccgac gcgaagctgg cgatgtggcc gccgacgttg
      301 gtctcgcggt tggcgcgcag caccatcatc gccgcgttcc agcgcacgaa cgaacggatg
      361 cggtgctcga tgtcctgatc gcccgggatc ggcgcctgct tctcgaccgg gacggtgttg
      421 acgtacgggg tgttggcgct gaacggggcc agatcaccgg ccagccgcgt gcgctcgatc
      481 agctcctcga tcagccagtg cgcccgctcg gcgcccacat gggcgatgac accgtcgagg
      541 gcagccagcc attcgcgggt ctcctgcgga tcgacatctt tgtattgttc ggcagtcatg
      601 acgaaacctc atggcatcga ggtgaatgtt gcgacggccg tcctcctcct cccgggaacc
      661 agacctcggg cctggacggc atcctttgtg tgtctgcaga tgctgctgcg tcgcctaccg
      721 ttgtcggtct gcagcgacgc tggctgcgcg tggatcactc gcgcccgccg atgcgcatcc
      781 taaaagacca tctatgtatt tttccacgat gtgaagtgcc gccatttcgc agagttttca
      841 cgatgtgaaa tggcgaaaac cgggttccga ccgcccggta accatagatc gtgaattttc
      901 gtgtgccgaa aatctgcgcc tgctccagcg cagggaaacg gctccccggg gtaccgcacg
      961 ctgcgccccc cctcgcctgt tcaggacttc gccatgacgc tacgccgcct gcgcatcggc
     1021 acccgcctcg ccctcggatt cggcgcgatc ctcgtgatcg tcgccgcgac gctgaccgct
     1081 gcgatgctgc acgacctcgc cggcatccag acggcgagcc gcgccgagcg gctgaacggc
     1141 tcgatcggtc tggtcatcgc ctcgatgcag cgcgagcagc gcgccgccga ggcgctggcg
     1201 acgctggtgg cgagccagcc ctcggtggtc gaggccttcg cggccgggga tcgcgagcgc
     1261 ctgctcgccg agttcggcgg cgcgttcgcg gcgctcaaaa ccgggcagaa cctcgaacag
     1321 ttccagttcc acgggcccgg cgcgatctcc ttcctgcgcg tgcaccagcc gcagcagcac
     1381 ggcgacgatc tctccgccgc gcgcaggacc atcgtcgacg cccagcagag cggcaaggcg
     1441 gtcgccgggc tcgaagccga cgccggcggc ttcggcctgc gcgcggtcgt gccgatccgc
     1501 cgcggcgagc agctgctcgg cacggtcgag ttcggcgtgg cgatgggtca gggcttcttc
     1561 gacgagctga tgcgctggta tctgctgcgc gcgcggctgg tgacgctcga tgacgccggc
     1621 cagctccggc gcctggccga caccgccggc aactggcccg acctcggcga ggccgaactg
     1681 cgcgccgccg ccaagggcac gccggcgatg ggcgacatca tcaccggcga cgcgcatctg
     1741 gcggtgctga tcatgccgat cgccgattac gccggccgca cggccggtgc gctggaagtg
     1801 ttccaggacc agaccctgtt cgagcagacc gcccggcaga tccgccagac cgcactcgcg
     1861 ctcggtgccg gcctgctgct gctcggctgc ctgctcgcct ggtggctggc gcgcggcatc
     1921 gcccggccgc tcggcgcgat ggccgaagcc gcgcaggcga tcgccgccgg cgacaccagc
     1981 tgcgaggtcc gctatgtcgg ccgcgacgag gtcggcaggc tcgccgatgc cttccgcgcc
     2041 atgctcgcct acctgcgcga gctgacgcag gcggtcgatg cgctcgatcg cggcgacacc
     2101 gctcgccgcc acacggcgcg cagtgaggcc gaccggctcg gcaccagcgt cgcgcgcacg
     2161 caggcgacgg tcgccgagct gctggccgaa acgcagtccc tggtgcacgc ggccgaggtc
     2221 ggcgagctgg cgcggcgcgg gcgcaccgag cgtttcagcg gcgactacgg cgcgctggtc
     2281 gcgagcgtca accacctgct cgacacccgc gcggccgccc gcgaacgcga gaccgccgcc
     2341 gaggccgaac gcctcgccgc cgcccgggcc gccaccgagc agctggccac gcaggtcgag
     2401 acgctgctcg cccgcgtcga tgccgccgcg cgtggcgacc tgaccgtgca ggtcgacggc
     2461 agcgatgaag acgccatcgg ccgggtcggt gccggactct cgcgcctgct cggcgacctg
     2521 cgcggccatc tcggcgccat cggcag
//
LOCUS       DAOQJH010000447         2529 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_29749_length_2529_cov_3.993129, whole genome shotgun sequence.
ACCESSION   DAOQJH010000447 DAOQJH010000000
VERSION     DAOQJH010000447.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2529
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29749_length_2529_cov_3.993129"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(106..828)
                     /gene="phoU"
                     /locus_tag="PLQ18_10115"
     CDS             complement(106..828)
                     /gene="phoU"
                     /locus_tag="PLQ18_10115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012536519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HNE02051.1"
                     /translation="MSVSTDGHTVKRFDQQLASLRSLVLEMGGLVEDQINRAVQALQD
                     EDIAAAREVVARDHVVNGFDVRADEEVVNLLALRQPVGSDLRMVLSMAKTVTDLERIG
                     DEAQKIARMVTQIYDGNSAPNARLLRDVANMARLAANMLHGALDALARMDVDKAVEIA
                     CGDEELDSEFQSALRRLVTFMMEDPRTIGHAIDVLFMIKALERIGDHSKNIAEYVVYL
                     VKGKDVRHVTIETLAAQGRNGS"
     gene            complement(865..1707)
                     /gene="pstB"
                     /locus_tag="PLQ18_10120"
     CDS             complement(865..1707)
                     /gene="pstB"
                     /locus_tag="PLQ18_10120"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015047843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HNE02052.1"
                     /translation="MPELHPQPEADHTAPAPVGHRTVGTVTVDNPKIAARDVNVFYGE
                     KHAIKHVDIDIGINEVIAFIGPSGCGKSTFLRCLNRMNDTIDGCRVEGAITLDGQDIY
                     DRELDVVQLRARVGMVFQKPNPFPKSIYDNVAYGPRIHGMASSRSELDDLVQWSLEKA
                     GLWNEVKDRLQQPGTGLSGGQQQRLCIARAIAVSPEVILMDEPCSALDPIATAKIEEL
                     IDELRENYSIVIVTHSMQQAARVSQRTAYFHLGDLIEVGETEQIFTNPVHKLTEGYIT
                     GRFG"
     gene            complement(1792..>2529)
                     /gene="pstA"
                     /locus_tag="PLQ18_10125"
     CDS             complement(1792..>2529)
                     /gene="pstA"
                     /locus_tag="PLQ18_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006968792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HNE02053.1"
                     /translation="LKLRFNRAFFANGDSRDPEQAGVRGALFGTFLTLLITLTLSFPI
                     GVAAAVYLEEFAPKNRMTDVIEVNINNLAAVPSIVFGLLGLAVFIAFFGMPRSAPLVG
                     GLVLTLLTLPTIIIASRAALKAVPPSIREAALGMGASRIQMVGHHVLPLALPGMLTGA
                     IIGMARAIGETAPLLMIGMVAFIVDVPGSLTAPATALPVQIYLWADSPERAFVERTSA
                     AIIVLLAFMVLMNLLAVILRRRFERRW"
BASE COUNT          387 a          819 c          893 g          430 t
ORIGIN      
        1 gaggcttgtc cggccatccg ccgcgcgcgg gcacgtcgga tcaacgtaga cggacccgcc
       61 ggtgcctgcc agcggtgcgc cggagccgcc ctgggcggcg tgcgttcagc tgccgttgcg
      121 gccctgggcg gcgagcgtct cgatcgtgac gtggcgcacg tccttgccct tgaccagata
      181 cacgacgtat tcggcgatgt tcttggagtg atcgccgatg cgctccagcg ccttgatcat
      241 gaacagcacg tcgatcgcat gcccgatcgt gcgcgggtcc tccatcatga aggtcaccag
      301 ccggcgcagc gccgactgga actcgctgtc gagctcctcg tcgccgcagg cgatctcgac
      361 cgccttgtcg acatccatgc gcgccagcgc atcgagcgcg ccgtgcagca tgttggcggc
      421 gaggcgcgcc atgttggcga catcgcgcag cagccgggca ttcggcgcgc tgttgccgtc
      481 gtagatctgc gtgaccatgc gcgcgatctt ctgcgcctcg tcgccgatgc gttcgaggtc
      541 ggtgaccgtc ttggccatcg acagcaccat gcgcaggtcc gagccgaccg gctggcgcag
      601 cgccagcagg ttgacgactt cctcgtcggc gcgcacgtcg aagccgttga cgacgtgatc
      661 gcgcgcgacc acctcgcgcg ccgcggcgat gtcctcgtcc tgcagcgcct gcacggcacg
      721 gttgatctgg tcctcgacga ggccgcccat ttcgagcacg agactgcgca ggctcgcgag
      781 ctgctggtcg aagcgcttga cggtgtggcc gtcggtactc acggacatgg cggatactcc
      841 ctgggggcga gcgggcaggg cggctcagcc gaagcggccg gtgatgtagc cttcggtcag
      901 cttgtgcacg gggttcgtga agatctgctc ggtctcgccg acctcgatca ggtcaccgag
      961 gtggaagtac gcggtgcgct gcgacacgcg tgcggcctgc tgcatcgagt gcgtgacgat
     1021 gacgatcgag tagttctcgc gcagctcgtc gatcagttcc tcgatcttcg cggtcgcgat
     1081 cgggtcgagc gccgagcagg gctcgtccat caggatcacc tcggggctga cggcgatcgc
     1141 gcgcgcgatg cagaggcgct gctgctggcc gccggaaagt ccggtgccgg gctgctgcag
     1201 gcggtccttg acctcgttcc agaggccggc cttctccagg ctccactgca cgaggtcatc
     1261 gagctccgaa cgcgagctgg ccatgccgtg gatgcgcggg ccgtaggcga cgttgtcgta
     1321 gatcgacttc gggaacgggt tcggcttctg gaacaccatg ccgacgcgtg cgcgcaactg
     1381 cacgacatcg agctcgcggt cgtagatgtc ctgaccgtcg agcgtgatcg cgccctcgac
     1441 gcggcagccg tcgatggtgt cgttcatgcg gttgaggcag cgcaggaacg tcgacttgcc
     1501 gcagcccgag gggccgatga aggcgatgac ttcattgatg ccgatgtcga tgtcgacgtg
     1561 cttgatcgcg tgcttctcgc cgtagaacac gttcacgtca cgcgcggcga tcttcgggtt
     1621 gtcgacggtc accgtaccga cggtgcggtg gccgacgggg gccggggcgg tgtggtccgc
     1681 ctcgggctgc gggtgaagct ccggcatcgg ggtttcctcg attcggttca gcggttccgg
     1741 tcgggtcatc gtagtgtctt ccggcatgca gatgcggtgg gtgtgctgcg cttaccagcg
     1801 gcgctcgaag cgccggcgca ggatcacggc gagcaggttc atcagcacca tgaatgccag
     1861 cagcacgatg atcgcggccg aggtgcgctc gacgaaggcc cgctcggggc tgtcggccca
     1921 gaggtagatc tgcacgggca gggccgtggc cggagcggtc aggctgcccg gcacgtcgac
     1981 gatgaacgcg accatgccga tcatcagcag cggcgcggtc tcgccgattg cgcgcgccat
     2041 gccgatgatc gcgccggtca gcatgccggg cagcgccagc ggcagcacgt gatggccgac
     2101 catctggatc cgcgatgcgc ccatgccgag cgcagcctcg cggatcgacg gcggcaccgc
     2161 cttcagcgcg gcgcgcgagg cgatgatgat ggtcggcagc gtgagcagcg tcagcaccag
     2221 gccgcccacc agcggcgcgg acctcggcat gccgaagaag gcgatgaaca ccgccagccc
     2281 gagcaggccg aacacgatcg agggcaccgc ggcgaggttg ttgatgttga cctcgatcac
     2341 gtcggtcatg cggttcttcg gcgcgaattc ctcgagatag accgcggccg ccacgccgat
     2401 cgggaacgac agcgtcagcg tgatcagcag tgtcaggaac gtgccgaaca gcgcgccgcg
     2461 aacgcccgcc tgctccggat cccgcgagtc gccgttggcg aagaacgcgc ggttgaagcg
     2521 cagcttcag
//
LOCUS       DAOQJH010000448         2526 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_29819_length_2526_cov_3.118980, whole genome shotgun sequence.
ACCESSION   DAOQJH010000448 DAOQJH010000000
VERSION     DAOQJH010000448.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2526
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29819_length_2526_cov_3.118980"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(288..773)
                     /gene="hypD"
                     /locus_tag="PLQ18_10130"
                     /pseudo
     CDS             complement(288..773)
                     /gene="hypD"
                     /locus_tag="PLQ18_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012494720.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
     gene            complement(770..1048)
                     /locus_tag="PLQ18_10135"
     CDS             complement(770..1048)
                     /locus_tag="PLQ18_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004381987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HNE02054.1"
                     /translation="MCLAIPARIVETLPDDEALVDLGGVRKRISLALVEDCAVGDYVI
                     VHVGYALNRLDPDEAARTLALFAEAGLLGDETGDDAGDGAGRDAEVRS"
     gene            1309..1725
                     /locus_tag="PLQ18_10140"
     CDS             1309..1725
                     /locus_tag="PLQ18_10140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017522436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HNE02055.1"
                     /translation="MEILYPAILEPQSGGGFCVRFVDFDEAFTEGETVEEAQFNAIDV
                     LTLTLEGRLEEGLPIPEPTPDLSGAVYIAPDARTQSALLIRRARGERSLADIARVLQT
                     TWPAAERLEDPRHWPSLRQLDRAAAALGKRLVLRFE"
     gene            complement(1764..2153)
                     /locus_tag="PLQ18_10145"
     CDS             complement(1764..2153)
                     /locus_tag="PLQ18_10145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02056.1"
                     /translation="MSKHAQHHLDAFSRRWLDLWTAVPVVVAIRVGRMMTADPSLSAR
                     DHREFTRMGSEKFDALGESWLAMVLQWQNECLRLAMTPWWLAPDALQQAGNEFGRSLA
                     KVAHQGLEPIERRASANARRLVRLRPR"
BASE COUNT          418 a          886 c          875 g          347 t
ORIGIN      
        1 tacgactcgt cgttccgcga ggccagcgaa cggcagtcga cgagtgacag cgcctattct
       61 cacgcgcgcg aacttgcccg cacagcccaa ttcatgcgcg aatggagcag cggcacacaa
      121 acagacttta cgaactacgc agcacgtcgc ctcgccgagc gtggcctcct gcgcgaggac
      181 gatccgatca gcttgcagcg cgcggtaact gaaatcgcct attcctatgc ccgcggcggg
      241 aattcgcata caggttacgt cccgaccaac agtccactta atccgtcgag cccttcggcc
      301 gcggcctggc ggatcgcgac cgcggtgggc ggcgtggtgg tctcgaagcc gatcgcgaag
      361 aagaccacct cgcggtctgg gtgtctgcgc gcgagtgcca gcgcgtcgtc gatcgaatag
      421 accatgcgca cgtcggcacc gcgcgcgcgc gccttcagca ggctgtcgcc gcccgagccg
      481 ggcacgcgca gcatgtcgcc gtagctcgcg agcatcacgc ccgggcgttc ggcgagtgcg
      541 atcgccatct ccagccggcc ggtcggcagc acgcataccg ggcagccggg gccgtggatc
      601 agccggacgt tggccggcag cagatcctcg acgccgtagc gggcgatcgc gtgcgtgtgg
      661 ccgccgcaga actccatgaa gcggtactcg cgcgccggat cgacctcgcg ggcgatcgcc
      721 gcggccagcc ggcgggccag cgcgccgtca cggaactcat cgacgtactt cacgaacgca
      781 cctccgcatc ccggccggcc ccgtcaccgg cgtcatcacc ggtctcgtca ccgagcagcc
      841 cggcctcggc gaacagcgcc agcgtgcgcg ccgcctcgtc cggatcgagc cggttcagcg
      901 cgtagccgac gtgcacgatc acgtaatccc cgaccgcaca gtcctcgacc agcgccagcg
      961 agatgcgctt gcgcacgccg ccgagatcga cgagtgcctc gtcatccggc agggtttcga
     1021 cgatgcgggc gggaatggcg aggcacatgg cgagcgttcc gtcagggagg acaggggatc
     1081 gggcggctgc atgcggaagc agggcagagc cggacgcagg cggtcgatcg tggcagaaag
     1141 gaaaccggcc gggtcacggc gtggatgcag gcgacgcgga gcggtgcgaa aacgtgcgtc
     1201 gggcgaaacg tacatcaccc gatacacgca cgacccggtc ccgggccgga tacgctggcg
     1261 ggcatcgggt gccagaccgg cgtccggctc aagcgctagg gcttcagcat ggaaattctg
     1321 tatccggcca ttctcgaacc gcagtccgga ggcggtttct gcgtgcgttt cgtcgatttc
     1381 gacgaagcct tcaccgaagg cgaaaccgtc gaggaagcgc agttcaacgc gattgatgtg
     1441 ctgacgctga cgctggaagg tcgtctggaa gaagggctgc cgattcccga gccgaccccc
     1501 gatctttccg gagccgtcta catcgccccc gatgcccgca cgcagtcggc gctgctgatc
     1561 cgccgggcac ggggggagcg ttcgctggcc gacatcgcgc gcgtgctgca gaccacctgg
     1621 ccggccgccg agcggctcga agacccgcgc cactggccga gtctgcgtca gctcgatcga
     1681 gccgccgcgg cgctcggcaa gcggctggtg ctgcgtttcg aatagcccgg gcagctcgga
     1741 cggcagcgaa ggcgcgcggc ggctcagcgc gggcgcagtc gcaccaggcg ccgggcgttg
     1801 gccgaggcgc ggcgctcgat cggctccagc ccctgatggg cgaccttggc cagcgagcgc
     1861 ccgaactcgt tgcccgcctg ctgcagcgca tcgggtgcca gccaccacgg cgtcatcgcc
     1921 agtcgcaggc attcgttctg ccactgcagg accatcgcca gccagctctc gccgagggca
     1981 tcgaacttct ccgagcccat gcgcgtgaat tcgcgatgat cgcgcgccga cagcgacggg
     2041 tcggcggtca tcatgcggcc gacgcggatc gccacgacga ccggtacggc cgtccacaga
     2101 tcgagccagc ggcgggaaaa ggcatcgagg tggtgctggg catgtttcga catgacgggg
     2161 ggactccggc tttcgggggg gattcggatc aatccggctg gccggatgag caatgcgggc
     2221 ctcgcgcgac gggctggcag aactgaccgc agccgccccc cggcggttcg gacgcaggcc
     2281 ggaatacttc ggcacgccct gcgcgcgcgc ggcccccgcg cggcccggaa cgccagcgga
     2341 cgagcggcct tcaggcatcg tcgcccccca tcgccaccca ggcctgcccg agcgccagcc
     2401 cgccgtcgtt cggcggcagc tcgcaggctt cgagcacgcg caggccggcg gcttcgagca
     2461 gcgcgcgcag gccgccgacc agcagcaggt tcgcgcagca gccgccggcg agcgcgacgg
     2521 tatcga
//
LOCUS       DAOQJH010000449         2519 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_29960_length_2519_cov_4.355925, whole genome shotgun sequence.
ACCESSION   DAOQJH010000449 DAOQJH010000000
VERSION     DAOQJH010000449.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2519)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2519)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2519
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29960_length_2519_cov_4.355925"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1382)
                     /locus_tag="PLQ18_10150"
     CDS             complement(<1..1382)
                     /locus_tag="PLQ18_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013916999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HNE02057.1"
                     /translation="MAATLHRGLEQRHITLMSLGAAIGVGLFLGSGAAIKLAGPAILI
                     AYAIGGLAMFFIMRALGEMAINQPVAGSFGRYAHEYLGPLAGYLTGWNYWFLWIVTAM
                     AEITAVGIYMQMWFPGSPAWLWALAAFVSMTAVNLIAVKAYGEFEFWFAAIKVVAIVA
                     MILIGGGVIFFGLGNGGEAVGISNLWAHGGFLPNGVTGVLMSLQMVMFAYLGIEMIGL
                     TAGEVKDPKKALSRAIDSVFWRILIFYVGALFVVMALYPWNEIGTQGSPFVLTFQKVG
                     IGGAAGIINFVVLTAALSSCNSGIYSTGRMLFNLAEQGQALPVFSRVSRGGVPVWAIL
                     GSSAMLLIGVLLNYLVPEKVFVWVTSISTFGAVWTWAMILCSQLRFRTLKGAPAEYRM
                     PWWPYSAWFSLGFLALVVGLLAWFPDTRIALYVGPLWLVLLVVLYHVLGHHRSAPQSG
                     SLPAALAADTA"
     gene            complement(1465..1842)
                     /locus_tag="PLQ18_10155"
     CDS             complement(1465..1842)
                     /locus_tag="PLQ18_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HNE02058.1"
                     /translation="MQIVSTPAAPAAIGPYAQGVIAAGLLYTSGQIPLDADGHFVAGD
                     IEVQTTQVLANLRAVIEAAGAGFADVIRTTVYVADLDDFPRLNAVYARHFGEHRPARS
                     TVQVARLPRDARVEIEAIVRLPG"
     gene            complement(2228..>2519)
                     /locus_tag="PLQ18_10160"
     CDS             complement(2228..>2519)
                     /locus_tag="PLQ18_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012978524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02059.1"
                     /translation="TMSDRSVLEAADFELADNAIHAGEEGSPSAPAAPVAAFSGDPAG
                     DGWFAAVERCERELLARLWPQFPSSRRLAAHLGTSHTMIAKKLRKYRIGRES"
BASE COUNT          475 a          833 c          849 g          362 t
ORIGIN      
        1 gccgtatccg ccgccagcgc agccggcaac gacccggact gcggggcact gcggtgatgg
       61 ccgagcacgt ggtacagcac caccagcagc accagccaga gcggaccgac gtagagcgcg
      121 atgcgggtat ccgggaacca ggccagcagg ccgacgacca gcgccaggaa gccgaggctg
      181 aaccaggcgc tgtacggcca ccacggcatg cggtattcgg ccggcgcgcc cttgagggtg
      241 cggaagcgca gctgcgagca caggatcatc gcccaggtcc acaccgcgcc gaaggtcgag
      301 atgctggtga cccagacgaa caccttctcg ggcacgaggt agttcagcag cacgccgatc
      361 agcagcatcg cgctggagcc gaggatcgcc cagaccggca cgccaccgcg cgacacccgg
      421 ctgaacaccg gcagcgcctg gccctgctcg gccagattga acagcatgcg cccggtgctg
      481 tagatgccgc tgttgcacga ggacagcgcg gcggtcagca cgacgaagtt gatgatgccg
      541 gccgcaccac cgatgccgac cttctggaag gtcagcacga acgggctgcc ctgcgtaccg
      601 atctcgttcc acggatacag cgccatgacc acgaacagcg cgccgacgta gaagatcagg
      661 atgcgccaga acaccgagtc gatcgcgcgc gacagcgcct tcttcgggtc cttcacctcg
      721 ccggcggtca ggccgatcat ctcgatgccc agatacgcga acatcaccat ctgcagcgac
      781 atcagcacgc cggtcacgcc gttcggcagg aagccgccgt gtgcccacag gttggagatg
      841 ccgaccgcct cgccgccgtt gccgaggccg aagaagatca cgccgccgcc gatcaggatc
      901 atcgcgacga tcgcgacgac cttgatcgcc gcgaaccaga actcgaactc gccgtaggcc
      961 ttcacggcga tcaggttcac cgccgtcatc gacacgaagg ctgcgagcgc ccacagccag
     1021 gccggcgagc cggggaacca catctgcatg tagatgccga cggcggtgat ctcggccatc
     1081 gccgtgacga tccacaggaa ccagtagttc cagccggtca ggtagccggc cagcgggccg
     1141 aggtattcat gcgcgtagcg gccgaaggag ccggcgaccg gctggttgat cgccatctcg
     1201 ccgagcgcac gcatgatgaa gaacatcgcg aggccgccga tcgcataggc gatcaggatg
     1261 gccgggccgg caagcttgat cgccgcgccc gagccgagaa acagcccgac gccgatggcg
     1321 gcgccgaggg acatcagcgt gatgtgtcgt tgttcgagac cacgatgcag ggtggctgcc
     1381 atggtgttgc tccgctaggt ggtgcctgca ggaagaaccg ccccggtctg tgggtgccgg
     1441 gtgcggaaag gcggcgcgcg gggatcagcc cggcagccgc acgatggctt cgatctcgac
     1501 gcgcgcatcg cgcggcaggc gcgcgacctg cacggtcgag cgcgccggcc ggtgctcgcc
     1561 gaagtgccgg gcatagacgg cgttcaggcg cgggaagtcg tcgaggtcgg cgacgtagac
     1621 ggtggtgcgg atcacgtcgg cgaagcccgc gccggcggcc tcgatcacgg cgcgcaggtt
     1681 cgcgagcacc tgcgtggtct ggacctcgat gtcgccggcg acgaagtggc cgtcggcatc
     1741 gagcggaatc tggccggacg tgtacagcag gccggcggcg atgacgccct gcgcgtaggg
     1801 gccgatcgcg gccggcgcgg cgggggtgga aacgatctgc atgggattct ggctccgtgg
     1861 atcgggacgg cccccggacg ggaggcgtaa ccatccttgg caatcccgat gccaggcatc
     1921 agaaacgctt gtatttcagc agcttgaagg atttttgctc gatccggcgc cggaccttcc
     1981 gtacacgatc gaatacagca gacggccggc caccggcaga accggcatca agttgatccg
     2041 gatcaatgac ttgatcggca gggaagcttc tgtaagcgat tggctacgcg tatgcgtccg
     2101 gctacagatg cgccgggtcc gctgtccatc cgctttcggc aagggtgatg ccagataata
     2161 tgatggtata aaaattaatc aaaccaagct tccagccttt ccatccggcc gtccggaacg
     2221 cccgccttca ggactcccga ccgatccggt acttgcgcag cttcttcgcg atcatcgtgt
     2281 gcgaggtgcc cagatgcgcg gcgagccggc ggctcgacgg gaactgcggc cacagccggg
     2341 ccagcagttc gcgctcgcag cgctcgacgg ccgcgaacca gccatcgccc gccggatcgc
     2401 cggaaaaagc cgccaccggc gccgccggtg cggacggcga cccttcctcg ccggcgtgga
     2461 tcgcgttgtc ggcgagttcg aagtcggcgg cttcgagcac gctgcgatca ctcatcgtc
//
LOCUS       DAOQJH010000450         2518 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_29987_length_2518_cov_2.735688, whole genome shotgun sequence.
ACCESSION   DAOQJH010000450 DAOQJH010000000
VERSION     DAOQJH010000450.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2518
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29987_length_2518_cov_2.735688"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="PLQ18_10165"
     CDS             complement(<1..237)
                     /locus_tag="PLQ18_10165"
                     /inference="COORDINATES: protein motif:HMM:NF021614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098003 - viral tail assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HNE02060.1"
                     /translation="MSDGCEIVTLRYPVEFGGRRIEELRLRRPRAGDLIATDRVQGAH
                     AKDAALAGHLAEVEPEVIRALDGADWMAVREVLAG"
     gene            complement(234..413)
                     /locus_tag="PLQ18_10170"
     CDS             complement(234..413)
                     /locus_tag="PLQ18_10170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02061.1"
                     /translation="MQEYEVKVACYFAGAEHAPGERVWLFQGQATYLVAGGFLVEVPS
                     APTPALADEKKGTKA"
     gene            complement(423..929)
                     /locus_tag="PLQ18_10175"
     CDS             complement(423..929)
                     /locus_tag="PLQ18_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019236446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HNE02062.1"
                     /translation="MVPYVLKNMNLLVDGFGYAGKVTVLTPPKLSRKVEEFRAGGMDG
                     SVELDMGLEKLEATFTLAEFDKGVLALWNLRDLDSVLLRFRGAIERDDTGDVRAVEVT
                     LRGRWKELDHGDWKPGEKAELKVTVACTFYSYTEDGVPLIVEDVVNMISLANGVDRLS
                     GLRTALGV"
     gene            complement(944..2467)
                     /locus_tag="PLQ18_10180"
     CDS             complement(944..2467)
                     /locus_tag="PLQ18_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018283447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HNE02063.1"
                     /translation="MPEQFLHGVEVVEIDTGPRPISTVRSSVIGIVGTAPAADGATQA
                     SLQTGVVTTKNALVWTAKSPAYAGAVGNAITVELKNPGTASAALSVTAVGTAITVSLA
                     TDAQKVITSTAALVLAAVNALPAAAALVTIARASGSDGTGVVGATAKVALAGGENQPF
                     PVDTPVLITSRREAARLGTSGTLPAAIDGIFDQAGAVVVVVRVTQAAGGDADATTVSN
                     VIGTVTGGGQYTGALALLGAEAAVGVVPRILIAPGYTQMQSVLSNFLGIADKLRAVVI
                     ADGPNTTDAAAIQYRQLWGSPRVYLVDPWVRVWDTTLSAERIEPASARVAGMISRSDN
                     DRGFWWSPSNQEMYGILGPARPVDFALGDVTSRANILNEAGLATIIQQQGFRLWGNRG
                     CAMDPKWAFLCVRRTADMINESLLRAHLWAVDRNITRTYVQDVVEGVNAYLRHLRAVG
                     AIIGGSCWADPALNTPDQIAQGKVYFDFDFSAAYPAEHITFRSHLVNDYLVEILKAA"
BASE COUNT          361 a          958 c          787 g          412 t
ORIGIN      
        1 acccgccagc acctcgcgca ccgccatcca gtccgcgcca tcgagcgcgc ggatcacctc
       61 cggctcgacc tcggccagat gcccggccag cgccgcgtcc ttcgcgtgcg ccccctgcac
      121 ccgatcggtc gcgatcaggt caccggcccg cggccggcgt aaccgcagtt cctcgatgcg
      181 ccggccgccg aactcgaccg gatagcgcag cgtcacgatt tcacacccgt cgctcatgcc
      241 ttcgtccctt tcttctcatc cgccaatgct ggcgtcggcg ccgatggcac ttccaccagg
      301 aacccgcccg cgaccagata cgtcgcctgc ccctgaaaca gccacacccg ctcgcccggt
      361 gcatgctcgg cacctgcgaa gtagcaggcc accttcacct cgtattcctg catcgcgccc
      421 ccttacacgc ccagcgccgt gcgcagaccg ctcagccgat ccacgccgtt cgccaggctg
      481 atcatgttca ccacgtcctc gacgatcagc ggcacgccgt cctcggtgta cgagtagaac
      541 gtgcaggcca ccgtcacctt cagctcggcc ttttcgcccg gcttccagtc gccgtgatcc
      601 agttccttcc agcgcccgcg cagcgtcacc tcgactgcac gcacgtcgcc ggtgtcatcg
      661 cgctcgatcg cgccccggaa gcgcagcagc acgctgtcca ggtcacgcag gttccacagc
      721 gccagcacgc ccttgtcgaa ctccgcgagc gtgaaggttg cctccagctt ctccaggccc
      781 atgtcgagct cgaccgagcc atccatgccg ccggccctga actcctcgac cttgcgactg
      841 agcttcggcg gcgtcagcac cgtcaccttg ccggcgtagc cgaagccatc gaccagcaag
      901 ttcatgttct tcagaacgta aggcaccacg attcagccct ccttcacgcg gccttcagga
      961 tttcgaccag gtagtcgttg accagatgcg agcggaacgt gatgtgctcc gccggatagg
     1021 ccgcgctgaa gtcgaagtcg aagtacacct tgccctgcgc gatctggtcc ggcgtgttca
     1081 gcgccggatc ggcccagcac gagccgccga tgatggcgcc gactgcgcgc aggtggcgca
     1141 ggtatgcgtt cacgccctcg accacgtcct gcacgtaggt gcgcgtgatg ttgcggtcga
     1201 ccgcccacag gtgggcacgc agcaggctct cgttgatcat gtcggccgtg cggcgcacgc
     1261 acaggaacgc ccacttcggg tccatcgcgc agccgcggtt gccccacagc cggaagccct
     1321 gctgctggat gatcgtcgcg aggccggctt cgttgaggat gttggcgcgg ctggtcacgt
     1381 cgccgagggc gaaatcgacc ggccgcgccg gcccgaggat gccgtacatc tcctggttgc
     1441 tcgggctcca ccagaacccg cggtcgttgt ccgagcggct gatcatcccg gccacgcgcg
     1501 cgctcgccgg ctcgatgcgt tcggccgaca gcgtcgtgtc ccagacgcgc acccacgggt
     1561 ccaccaagta gacgcgcggg ctgccccaga gctggcggta ctggatcgcc gcggcgtccg
     1621 tggtgttcgg gccgtcggcg atcacgaccg cgcgcagctt gtcggcgatg ccaaggaagt
     1681 tcgacagcac cgactgcatc tgcgtgtagc cgggcgcgat caggatgcgc ggcaccacgc
     1741 ccacggccgc ctcggcgccg agcagcgcca gcgcgccggt gtactgcccg ccgccggtca
     1801 cggtgccgat gacgttgctg accgtcgtcg cgtccgcatc gccgccggca gcctgcgtca
     1861 cgcgcaccac gacgacgacc gcgccggcct ggtcgaagat gccgtcgatc gccgccggca
     1921 gcgtgccgct ggtgccgagc cgcgccgcct cgcgccgcga ggtgatcagc accggcgtat
     1981 cgaccgggaa gggctggttt tcgccgccgg cgagcgcgac cttggccgtg gccccgacca
     2041 cgccggtgcc gtccgagccg ctcgcgcgcg cgatcgtcac cagcgctgcc gctgccggca
     2101 gcgcgttgac cgccgccagc accagcgcgg ccgtcgacgt gatcaccttc tgtgcatcgg
     2161 tcgcgagcga cacggtgatc gcggtgccga ccgctgtcac gctcagcgcc gcgctcgcgg
     2221 tgccggggtt cttcagctcg acggtgatcg cgttgccgac cgccccggcg taggccggcg
     2281 acttcgccgt ccacaccagc gcgttcttcg tggtcacgac cccggtctgc aggctcgcct
     2341 gcgtcgcacc gtcggccgcc ggcgcggtgc cgacgatgcc gatgacgctc gaacggacgg
     2401 tgctgatcgg gcgcgggccg gtgtcgatct cgacgacttc gacgccgtgc aggaattgct
     2461 cgggcatctt ctgtatctcc ttcagctcag gatttcatcg gcccgcgcct gcgtcagc
//
LOCUS       DAOQJH010000451         2514 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_30079_length_2514_cov_2.642944, whole genome shotgun sequence.
ACCESSION   DAOQJH010000451 DAOQJH010000000
VERSION     DAOQJH010000451.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2514
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30079_length_2514_cov_2.642944"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..728)
                     /locus_tag="PLQ18_10185"
     CDS             complement(<1..728)
                     /locus_tag="PLQ18_10185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNE02064.1"
                     /translation="MPTVPAKSRSPALPPRLLAAIRAEQQAGERLIGQVQLAVVLTFG
                     LLWAIAPSPPSPAPSPVPWALAGYLLVTLTRLAWTQRRRLPDWAAYLSIVADFALLYG
                     LIWSFHVQYMQPPAFSLKAPTLLYAFIFVVLRVLRVELRFVIAAGAVAVAGWAALVGL
                     AIGGNLHDAAITRDYVHYLTANAVLLGGEFDKLATMLTVTLILALAVARGQRLLVRAT
                     VESNAAMQLSRFLPMAVAVRIAAAT"
     gene            1187..2305
                     /gene="ald"
                     /locus_tag="PLQ18_10190"
     CDS             1187..2305
                     /gene="ald"
                     /locus_tag="PLQ18_10190"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009023204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HNE02065.1"
                     /translation="MQIGVPKEIKNHEYRVGLTPASVRELVAHGHTVFVEHDAGLGIG
                     CDDAQYLAAGATVLGTAAEVFERAAMIVKVKEPQPVECRRLRAGQLLFTYLHLAPDPE
                     QTRLLVESGVTAIAYETVTGARGGLPLLAPMSEVAGRMAIQAGAHALEKAHGGRGVLL
                     GGVPGVAPAQVTVIGGGVVGAGAIRMAVGMGAHVTVLDRDIDRLRALDIEYGPRLTTL
                     FATRDAIEAQVLESDLVVGAVLVPGAAAPKLVTRAQLAQMRPGAVLVDVSIDQGGCFE
                     TSHPTTHAEPTYVVDGIVHYCVANMPGAVPRTSTFALNNATLPYTLALADKGFPQALF
                     DDAGLMAGLNVHAGRITCRAVAEALDYECVEPGEWLRG"
BASE COUNT          384 a          919 c          886 g          325 t
ORIGIN      
        1 gtggcggcgg cgatgcgcac ggccaccgcc atcggcagaa agcgcgacag ctgcatcgcg
       61 gcgttggact cgaccgtcgc ccgcaccagc agccgctgcc cgcgcgcgac cgcgagcgcg
      121 aggatcagcg tcacggtcag catggtcgcg agcttgtcga actcgccgcc gagcagcacg
      181 gcgttggcgg tcaggtaatg cacgtagtcg cgcgtgatcg ccgcatcgtg caggttgccg
      241 ccgatcgcga gcccgaccag cgccgcccag ccggccaccg ccaccgcacc ggccgcgatc
      301 acgaagcgca gctcgacgcg cagcacgcgc agcacgacga agatgaacgc atacagcagc
      361 gtcggcgcct tcagcgagaa cgccggcggc tgcatgtact ggacgtggaa gctccagatc
      421 agcccgtaca gcagcgcgaa gtcggcgacg atcgacaggt acgccgccca gtccggcagc
      481 cgccggcgct gcgtccacgc cagccgcgtc agcgtcacca gcagatagcc ggccagcgcc
      541 cacggcaccg gactcggcgc cggactcggc gggctcggtg cgatcgccca cagcaggccg
      601 aaggtcagca ccacggccag ttgcacctgg ccgatcagcc gctcgccggc ctgctgctcg
      661 gcgcggatcg cggcgagcag gcgcggcggc agcgcggggc tgcgggattt cgcggggacg
      721 gtcggcatcg ggcgacactc cgggcacggg acaggcaagg cacttcaggg accgtgccag
      781 cgcctgcagg ccggcatttg tctgtccgat ccgccacttc cgcgcctgcg cgggcggtcg
      841 tcgaccatga ccgcccgcgc cgcgtcgtgg cccgccactg ccgatcgcag ccacgaatcc
      901 gtgcggatcg ccgatccgtg cctgccgcgc agggggcatc ccttgccacg catcgaagtg
      961 cagatgccgc gcatcggtcg tcccttgcca cgcagggaag cgcccctgct tgccggacgg
     1021 cttgcggact gccgcgacca cgcggccgtg cgcgctggcc atgaatctgt ccagccgtca
     1081 aatctgcttg ccagccggcc ctgccggccg caaacgcgcg gcgcatgctt gccgccacgc
     1141 cacgaccgca gcccgtgcgg ccggcgattg caggagtgcc cgtgccatgc agatcggtgt
     1201 cccgaaagag atcaagaacc acgaataccg ggtcggactg acccccgcga gcgtgcgcga
     1261 gctggtcgcg cacggccaca cggtattcgt cgaacacgat gccggactcg gcatcggctg
     1321 cgacgatgcg cagtacctcg ccgcaggcgc cacggtcctc gggacggccg cggaagtctt
     1381 cgaacgcgcc gcaatgatcg tgaaggtcaa ggaaccgcag ccggtcgagt gccggcgcct
     1441 gcgcgccggg cagctgctgt tcacttacct gcatctggcc ccggacccgg aacagacccg
     1501 gctgctggtc gaatccggcg tcaccgccat cgcctacgaa accgtcaccg gtgcacgcgg
     1561 cggcctgccg ctgctcgcgc cgatgtccga agtcgccggc cgcatggcga tccaggccgg
     1621 cgcgcatgcg ctggaaaagg cccacggcgg ccgcggcgtg ctgctcggcg gcgtgcccgg
     1681 cgtggcgccg gcgcaggtga ccgtgatcgg cggcggcgtg gtcggggccg gcgcgatccg
     1741 catggcggtc ggcatgggcg cgcacgtcac cgtgctcgat cgcgacatcg atcggctgcg
     1801 cgcgctcgac atcgaatacg gcccgcgcct gacgacgctg ttcgcgaccc gcgacgcgat
     1861 cgaggcgcag gtgctcgaat ccgatctggt ggtcggggcg gtgctggtgc cgggcgcggc
     1921 ggcgccgaag ctggtcacgc gcgcgcagct cgcacagatg cgccccggcg cggtgctggt
     1981 cgatgtctcc atcgaccagg gcggctgctt cgagacctcg cacccgacca cgcacgccga
     2041 accgacctac gtcgtcgacg gcatcgtgca ttactgcgtc gccaacatgc cgggcgcggt
     2101 gccgcgcacc tcgaccttcg cgctgaacaa cgccacgctg ccgtacacgc tggcactggc
     2161 cgacaagggc ttcccgcagg cgctgttcga cgatgcgggc ctgatggccg ggctgaacgt
     2221 gcacgccggg cgcatcacct gccgcgcggt cgccgaggcg ctcgactacg agtgcgtcga
     2281 gccgggcgaa tggctgcgcg gctgaagccg ggcttgcgca ggccggactg tcgatctccc
     2341 cttgcccccg cgggcatggc tatctacaca acttgcagca gcggctcccg gactcgcccg
     2401 ggcagcgcgg atggacccgc acgacggcaa ggcattggcg aacaagcccc tcccccctgg
     2461 cgggggaggg gttggggaga gggggcaagg tccgggcgca gtgccggcgt ctcc
//
LOCUS       DAOQJH010000452         2506 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_30258_length_2506_cov_3.220726, whole genome shotgun sequence.
ACCESSION   DAOQJH010000452 DAOQJH010000000
VERSION     DAOQJH010000452.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2506)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2506)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2506
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30258_length_2506_cov_3.220726"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1283
                     /locus_tag="PLQ18_10195"
     CDS             <1..1283
                     /locus_tag="PLQ18_10195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02066.1"
                     /translation="IRFRLDPGSTASEDGETIVLGRQNGTGGGWGQLGQAALASGAFP
                     VGLAPRALKRTQAEIYNQRLWTLPEFHAARPDVIARCTVQARIPTALPATVCTRAYDY
                     VNVDGGTIDNEPFEIGRRTLAEDHGREHTPRSPHEADCAVLMIDPFPCPPVFTEDYQV
                     PEGLKLIPAIKHLLSAAIQQMRFKLPELMLADHPDVYSRFLITPVRYAADGRRVQLPG
                     TPIACGTLGGFGGFLNRAFRAHDYLLGRLNCQQFLRRHFVLAGDNPLFGGTAGAGSEY
                     DRLYGFEDRYDQKPVQLVPILPLFGSAAGAAIDGFQPQAPFGLPWPQIRESELQPVQG
                     GLKTRADAVVGALLKENVRSVLGRLALNSAWSVFGRRRVLDKAMDMIRDALAVAGLLA
                     GVAPPPVPTGGPDRTLPPVRSLDAGAPGGKVSNG"
     gene            1436..2062
                     /locus_tag="PLQ18_10200"
     CDS             1436..2062
                     /locus_tag="PLQ18_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004717645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase subunit alpha"
                     /protein_id="HNE02067.1"
                     /translation="MFTGIVQAAVPVVALVERPQLVTLTLELPERLCTGLAIGSSVAV
                     NGTCLTITGFEGRLVHFDAMLETLRVTNLGRLAVGDRVNVERAARHGDEIGGHVLSGH
                     VHALAEIIRIERPENNHEVVFRAPAALMKYLLPKGFVALNGCSLTIGRVEGDTFDVWL
                     IPETLRATTYGELQVGALVNLEIDAQTQAIVDTVERVLAERGLLAAAG"
     gene            2165..>2506
                     /locus_tag="PLQ18_10205"
     CDS             2165..>2506
                     /locus_tag="PLQ18_10205"
                     /inference="COORDINATES: protein motif:HMM:NF014715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - glucose binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HNE02068.1"
                     /translation="MSSSASDRTPDLIADIGGTNARFALSDGPGSTPRAVQVLATSAY
                     ADLAGAADAYLQHAGGPRPQRAAVAIANPVTGDRVEMTNNAARFSREATRTALGLERL
                     ELLNDFKALALS"
BASE COUNT          358 a          954 c          830 g          364 t
ORIGIN      
        1 gcatccgctt ccggctcgac cccggcagca cggccagcga ggacggcgag accatcgtgc
       61 tcggccgcca gaacggcacc ggcggcggct ggggccagct cggccaggcc gcgctcgcga
      121 gcggcgcctt cccggtcggc ctcgcgccgc gggcgctcaa gcgcacgcag gccgagatct
      181 acaaccagcg gctgtggacg ctgcccgagt tccatgcggc ccgacccgat gtcattgccc
      241 gctgcaccgt acaggccaga atcccgaccg cgctgccggc cacggtctgc acgcgcgcct
      301 acgactacgt caacgtcgat ggcggcacca tcgacaacga gcccttcgag atcggccggc
      361 gcacgctcgc cgaagaccat ggccgcgaac acaccccgcg cagcccgcac gaggccgact
      421 gcgcggtgct gatgatcgac ccgttcccgt gcccgccggt gttcaccgag gactaccagg
      481 tgcccgaggg cctgaagctg atcccggcga tcaagcacct gctcagcgcc gcgatccagc
      541 agatgcgctt caagctgccc gagctgatgc tcgccgatca tccggatgtc tacagccgct
      601 tcctgatcac gccggtgcgc tacgcagccg acggcaggcg cgtgcagctg cccggcacgc
      661 cgatcgcgtg cggcacgctc ggcggcttcg gcggcttcct gaaccgcgcc ttccgcgccc
      721 acgactacct gctcgggcgc ctgaactgcc agcagttcct gcgccggcac ttcgtgctcg
      781 ccggcgacaa cccgctgttc ggcggcacgg ccggcgccgg gtccgaatac gaccgcctgt
      841 acggttttga ggaccgctac gatcagaaac cggtgcaact ggtcccgatc ctgccgctgt
      901 tcggcagcgc cgccggtgcg gcgatcgacg gcttccagcc gcaggctccg ttcggcctgc
      961 cgtggccgca gatccgcgaa tccgagctgc aaccggtgca gggcggcctg aagacccgcg
     1021 ccgatgccgt ggtcggggcg ctgctgaagg agaacgtcag atccgtcctc gggcgcctgg
     1081 cgctgaacag cgcctggagc gtgttcggcc gtcgccgcgt gctcgacaag gccatggaca
     1141 tgatccgcga cgccctcgcc gtcgccggcc tgctcgccgg cgttgcgccg ccaccggtgc
     1201 cgaccggtgg tccggaccgc acgctgccgc cggtgcgcag cctggatgcc ggggcgccgg
     1261 gcgggaaggt cagcaacggc tgaacgtctg ctccgggcct catcggcagc ggctgccgac
     1321 accccgcccg cgctaccatc cgcaggaccc gcttcctgtt gcggtgcagc gcccccgtgc
     1381 gaccggcacg cacgggtccg cagcgccgcc cgccccgtcc tccggaggta tcgccatgtt
     1441 caccggcatc gtccaggccg ccgtgcccgt cgtcgcgctc gtcgagcgtc cgcaactcgt
     1501 caccctgacg ctggagctgc ccgagcggct gtgcaccggg cttgcgatcg gctccagcgt
     1561 cgcggtcaac ggcacctgcc tgaccatcac cggcttcgag ggccggctcg tgcatttcga
     1621 tgcgatgctg gagacgctgc gcgtgaccaa cctcggccgg ctcgcggtcg gggatcgcgt
     1681 caacgtcgag cgcgcggcgc gccacggcga cgagatcggc ggccacgtgc tgtcgggcca
     1741 tgtgcatgca ctcgccgaaa tcattcgcat cgagcggccc gagaacaatc acgaagtcgt
     1801 gttccgggcg ccggcggcgc tgatgaagta cctgctgccg aagggcttcg tggcgctgaa
     1861 cggctgcagc ctgaccatcg gccgcgtcga gggcgacacc ttcgatgtct ggctgattcc
     1921 ggaaaccctg cgcgccacca cctacggcga actgcaggtc ggcgcactcg tgaacctcga
     1981 gatcgacgcc cagacccagg ccatcgtcga taccgtcgag cgcgtgctcg ccgagcgcgg
     2041 gctgctggca gcggccggct gaacgacgga cgcggaacac cggcgcccgc agggcgtcgg
     2101 tatccggcaa tcccttcgct cctccccttc ctttcttccc tccccgctgc cgagtccgtt
     2161 gcccatgtct tcgagcgctt ccgaccgcac gcccgacctg atcgccgaca tcggcggcac
     2221 caatgcccgt ttcgcgctca gcgacggccc gggcagcacg ccgcgcgccg tgcaggtgct
     2281 cgcgacctcg gcctacgccg atctcgccgg ggcggccgac gcctatctgc agcacgccgg
     2341 cgggccgcga ccgcagcggg cggcggtggc gatcgccaat ccggtcaccg gcgatcgcgt
     2401 cgagatgacc aacaatgccg cgcgcttctc gcgcgaggcg acgcgcacgg cgctcggtct
     2461 tgagcggctc gaactgctga acgacttcaa ggcgctggcg ctgtcg
//
LOCUS       DAOQJH010000453         2503 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_30316_length_2503_cov_4.294935, whole genome shotgun sequence.
ACCESSION   DAOQJH010000453 DAOQJH010000000
VERSION     DAOQJH010000453.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2503)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2503)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2503
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30316_length_2503_cov_4.294935"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1063)
                     /locus_tag="PLQ18_10210"
     CDS             complement(<1..1063)
                     /locus_tag="PLQ18_10210"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015046137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HNE02069.1"
                     /translation="MTRAQIENVNVVSTEMLLTPAELHKRLPLTADARAAVVAGRETV
                     EAILDRRDPRLFVVVGPCSIHDPAAAMDYARRLKALSDELGDTLYLIMRVYFEKPRTT
                     TGWKGLINDPDMDDSFKIQKGLGVARQLLTDIAELGLPTGTEALDPISPQYLSDLITW
                     AAIGARTTESQTHREMASGLSTPVGFKNGTDGGLAVAINALLSVSKPHSFLGIDGEGQ
                     CSIIRTRGNRYGHVVLRGGPKPNYDSVTIALCEQELAKNKLPANIVVDCSHANSSKKH
                     ELQPLVLNDVVNQIVDGNRSILGVMIESNLNAGNQPIPADLSQLKYGVSVTDACVDWE
                     TTERMLRDAHAKLKDVLPKR"
     gene            complement(1169..2122)
                     /locus_tag="PLQ18_10215"
     CDS             complement(1169..2122)
                     /locus_tag="PLQ18_10215"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HNE02070.1"
                     /translation="MRELNSSVQPAATHRLPSLIALRAFEAAARLGSFQAAAAELHVT
                     PSAISHQIRALEGELGVPLFERLDKRVVLARGGQELFPAVREAMELLQAASLRVRRLA
                     GERRLVLSSAPLFATRWLLPRLADFHARHPDWELHVRATLEEEDFRSGEIDLAIRWGD
                     RDRHWPQHLAHEELLIERITAVASPDLAARLAPGSGPGALIDGAPLLASAFRDAEWQR
                     WFDAAGCLKPAPLPSAHFASTAHMLEAAVAGMGWALVDPRFAGRELAQGLLVAPWPLA
                     GCEGQHFHAVCPPAHTGRPGVARFVSWVRERLAAEAPATAG"
     gene            2281..>2503
                     /locus_tag="PLQ18_10220"
     CDS             2281..>2503
                     /locus_tag="PLQ18_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012881596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX system Lon protease-like protein BrxL"
                     /protein_id="HNE02071.1"
                     /translation="MERDALDDKLIAVFSGKVVRKDLLHQIKGGENVPSYVLEYLLGR
                     YCASDDEDEIRLGIETVKDTLRKNYFRQDE"
BASE COUNT          382 a          835 c          900 g          386 t
ORIGIN      
        1 ggcgcttcgg cagcacgtcc ttcagcttcg cgtgcgcgtc gcgcagcatg cgctcggtgg
       61 tctcccagtc gacgcaggcg tcggtgaccg agacgccgta tttgagctgc gagagatcgg
      121 ccgggatcgg ctggttgccg gcgttgaggt tgctttcgat catgacgccg aggatcgagc
      181 ggttgccgtc gacgatctgg ttgacgacat cgttcagcac cagcggctgc agttcgtgct
      241 tcttgctgga gttggcgtgg ctgcagtcga cgacgatgtt ggccggcagt ttgttcttcg
      301 ccagttcctg ctcgcagagg gcaatggtga ccgagtcgta gttcggcttg ggaccgccgc
      361 gcagcacgac gtggccgtag cggttgccgc gcgtgcggat gatcgagcac tgaccctcgc
      421 cgtcgatgcc gagaaaactg tgcggcttcg acaccgacag cagcgcgttg atcgccaccg
      481 ccagtccgcc gtcggtgccg ttcttgaagc cgaccggcgt cgacagcccc gaggccatct
      541 cgcggtgcgt ctgcgattcg gtcgtgcgcg cgccgatcgc cgcccaggtg atcaggtccg
      601 acaggtactg cgggctgatc gggtcgagcg cctcggtgcc ggtcggcagg ccgagttcgg
      661 cgatgtcggt cagcagctgc cgggcgacgc cgaggccctt ctggatcttg aaggaatcat
      721 ccatgtccgg gtcgttgatc aggcccttcc agccggtggt cgtgcgcggc ttctcgaagt
      781 acacgcgcat gatcaggtac agcgtgtcgc cgagttcgtc ggacagcgcc ttcaggcgcc
      841 gcgcgtagtc catcgccgcc gccgggtcgt ggatcgagca cgggccgacg acgacgaaca
      901 ggcgcgggtc acggcggtcg aggatggcct cgacggtctc gcgaccggcg acgacggcgg
      961 cgcgggcgtc ggcagtcagc ggcaggcgct tgtgcagttc cgcgggggtc agcagcatct
     1021 cggtcgagac gacgttgacg ttttcgatct gggctctggt catggccgca cttccctgga
     1081 aggggggagg agaaaacggc tgcggacagc cggttaggcg tgcgatacgc aacgggatgc
     1141 gcggtttagc gcgcgtgcgc ggcaggcgtc aaccggcagt tgccggagcc tcggccgcca
     1201 ggcgctcgcg cacccagctc acgaagcgcg ccacgcccgg gcggccggtg tgcgccggcg
     1261 ggcagacggc gtggaagtgc tggccctcgc agccggcgag cggccacggc gccaccagca
     1321 ggccctgtgc cagctcgcgc ccggcgaagc gcggatcgac cagcgcccag cccatgccgg
     1381 cgaccgcggc ttcgagcatg tgtgcggtgc tcgcgaagtg cgcactcggc agcggcgccg
     1441 gcttcaggca gccggcggca tcgaaccagc gctgccattc ggcgtcgcga aaggcgctgg
     1501 caagcagcgg ggcgccgtcg atcagtgcgc cgggaccgct gcccggagcg agccgcgcgg
     1561 ccagatcggg gcttgcgacc gcggtgatgc gctcgatcag cagctcctcg tgcgcgaggt
     1621 gctgcggcca gtgccggtcg cggtcgcccc agcggatcgc cagatcgatc tcgccgctgc
     1681 ggaagtcctc ctcttccagc gtcgcgcgca cgtgcagctc ccagtccggg tggcgggcgt
     1741 ggaagtcggc caggcgcggc agcagccagc gcgtcgcgaa cagcggcgcg ctcgacagca
     1801 ccagccgccg ctcgccggcg agccggcgca cgcgcaggct cgccgcctgc agcagctcca
     1861 tcgcctcgcg cacggccgga aacagctcct gcccgccgcg cgccagcacg acgcgcttgt
     1921 cgaggcgctc gaacagcggc acgccgagtt cgccttcgag cgcgcggatc tggtggctga
     1981 tcgccgaggg cgtcacatgc agctccgccg ccgcggcctg gaagctgccg aggcgcgcgg
     2041 cagcctcgaa ggcgcgcagc gcgatcagcg acggcagccg gtgcgtcgcg gccggctgca
     2101 cagatgaatt cagttcacgc atcgggaaaa ttcgtcgttt gtcgcgggtg tgacaagtcc
     2161 atgatcctgc gggcgtcatc caacctgtca ggagatgccg ccatgcagca tacgcttgaa
     2221 tcccgcgaca gcatccgccg cctcgccaac ggcacgatcg accacgggga gggctagccg
     2281 atggagcgcg acgcgctgga cgacaagctg atcgcggtgt tctcaggcaa ggtggtccgc
     2341 aaagacctcc tccaccagat caagggcggg gagaacgtcc catcctacgt gctggagtac
     2401 ctgctcggcc gctactgcgc ctccgacgat gaggacgaga tccgcctcgg catcgagacg
     2461 gtcaaggaca cgctgcgcaa gaactacttc cgccaggacg agg
//
LOCUS       DAOQJH010000454         2496 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_30465_length_2496_cov_4.133961, whole genome shotgun sequence.
ACCESSION   DAOQJH010000454 DAOQJH010000000
VERSION     DAOQJH010000454.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2496)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2496)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2496
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30465_length_2496_cov_4.133961"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..437)
                     /locus_tag="PLQ18_10225"
     CDS             complement(<1..437)
                     /locus_tag="PLQ18_10225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HNE02072.1"
                     /translation="MPIPRDETRAARARADGLDLKRLLLRRVTLFALAICLGGIVTVL
                     VQTQAELRRQVARIGPTLLERLINEEAMQPRGSFLMGLEELKLASLDPLGEWLDICVE
                     AEDLFGRQRVRRCFGERLALPAALRGLPALLLDGRELYRGPII"
     gene            complement(726..1967)
                     /locus_tag="PLQ18_10230"
     CDS             complement(726..1967)
                     /locus_tag="PLQ18_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNE02073.1"
                     /translation="MNSLFWPGRPFVLAATLACALLSACSVPARLPAVPAEQTTQATV
                     PGMVNVRYWPDVDTAALERLSLETIRRMQAYRAAQGQTGPLPPAHFLALSGGGDDGAF
                     GAGLLNGWTATGTRPSFGIVTGISTGALIAPFAFLGPAYDPVLRKVYTETRQADIFLT
                     RPVFAAINNDAMADTQPLWTLIGQNVDEDFLARIGAEYRKGRLLLIATTDIDARRPVL
                     WDMGAIATSGAPGALDLFRRIMLASAAIPGVFPPVLIDVEVDGKSHQEMHVDGGAAAQ
                     VFLYPPSLNVKVLSKRYGIERERHAWIIRNARLDPSWASVERRTMSIAGRAVSSLIQY
                     QGVGDLYRIYATTQRDGVDYNLAYIGSDFRVKHRDDFDPEYMRPLYEYGERLARNGYP
                     WQKTPPGYIAPPPAKTMVVQH"
     gene            2269..2448
                     /locus_tag="PLQ18_10235"
     CDS             2269..2448
                     /locus_tag="PLQ18_10235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02074.1"
                     /translation="MNHTIPENLTVAVETTARLLGEIRRQRNLRQQMSDPSMRTNAAT
                     RQPPVPHHGSPGDTH"
BASE COUNT          386 a          849 c          878 g          383 t
ORIGIN      
        1 tggatgatcg gaccgcggta cagctcgcgg ccatcgagca gcagcgccgg caggccgcgc
       61 agcgccgccg gcagcgccag gcgctcgccg aagcagcggc gcacgcgctg gcggccgaag
      121 aggtcctcgg cctcgacgca gatgtccagc cattcgccga gcgggtccag cgacgcgagt
      181 ttcagctcct ccagccccat caggaagctg ccgcgcggct gcatggcttc ctcgttgatc
      241 agccgctcca gcagcgtcgg cccgatgcgc gcgacctgcc ggcgcagttc ggcctgcgtc
      301 tgcaccagca ccgtgacgat gccgccgagg cagatcgcca gcgcgaacag cgtcacgcgc
      361 cgcagcagca ggcgcttgag gtcgagcccg tctgcccgtg cgcgcgcagc gcgggtttcg
      421 tcacggggga tcggcatcgt ggccacctgt ctccgggctg tcgggttccg ggaaatcatg
      481 aagcagtccg ggcgcgggga aagggcaggg gacggacgcg ggcttgccgg cccgcaatcg
      541 cgagggacgg agggcttttt tgaggtccgg gatgcctcgc cgccccgggg ctttcagggc
      601 cggggcgggc gggaagtggg gggcgagccg gggcggcggg tgctgtccgg cacgtccggc
      661 cggacggcaa tggcctgctg gcgaggctgg cagccggtgc gccggtcgcg gattgcccgc
      721 gcctgtcagt gctggaccac catcgtcttt gccggcggcg gtgcgatgta gcccggcggc
      781 gtcttctgcc acggatagcc gttgcgggcg aggcgttcgc cgtattcgta cagcggacgc
      841 atgtactcgg ggtcgaagtc gtcgcggtgc ttgacccgga agtcgctgcc gatataggcg
      901 aggttgtagt cgaccccgtc gcgctgcgtg gtcgcgtaga tgcggtacag atcgccgacg
      961 ccctggtact ggatcagcga cgacaccgcc cggccggcga tgctcatcgt gcggcgctcg
     1021 acgctggccc aggacggatc gagccgggcg ttgcggatga tccacgcatg gcgctcgcgc
     1081 tcgatgccgt agcgcttcga cagcaccttc acgttcagcg acggcggata caggaacacc
     1141 tgcgcggcgg caccgccgtc gacgtgcatc tcctgatggg acttgccgtc gacctcgaca
     1201 tcgatcagca ccggcgggaa cacgcccggg atcgccgccg aggccagcat gatgcgccgg
     1261 aacagatcga gcgcgccggg cgcaccgctc gtggcgatcg cgcccatgtc ccacagcacc
     1321 ggccggcggg cgtcgatgtc ggtggtcgcg atcagcagca gccggccctt gcggtattcg
     1381 gcgccgatgc gtgcgaggaa gtcctcgtcg acgttctgcc cgatcagcgt ccagagcggc
     1441 tgcgtgtcgg ccatcgcatc gttgttgatc gccgcgaaca ccggccgcgt caggaagatg
     1501 tccgcctgcc gggtctcggt gtagaccttc ctcagcaccg ggtcgtaggc cgggccgagg
     1561 aacgcaaacg gcgcgatcag cgccccggtg ctgatgccgg tgacgatgcc gaagctcggg
     1621 cgcgtgccgg tggccgtcca gccgttcagc agcccggccc cgaaggcacc gtcatcgcca
     1681 ccccccgaca gcgcgaggaa atgcgccggc ggcagcggtc cggtctggcc ctgggccgcg
     1741 cggtatgcct gcatgcgccg gatggtttcg aggctgagcc gctccagggc ggcggtgtcg
     1801 acatccggcc agtaacggac gttgaccatg ccgggcacgg ttgcctgcgt ggtctgttcg
     1861 gcgggcacgg caggcagccg tgccggcacc gaacaggccg acagcagcgc gcatgccagg
     1921 gtggcagcca gcacgaacgg ccggccgggc cagaagagac tgttcaaggc ggttgtcctc
     1981 gatgcgatac cagcgcctac ggtggcatgc cgagcgtaga catgtctcgc gcgcgtgccc
     2041 ggggccgcgc atggcttgcg tgtccgtgcc ggcggtgcag ccccgcggac tcgcccgcag
     2101 ccttgcctca gtccgcagac gaacggtgtt gcaacttctt tcgtacatta cgaatacgac
     2161 agatatcgta ttgagagaag tcgcagtggt aagcaatgct gtcgttgtac agtgtttctg
     2221 acagccaatc agctgtttag ccgccttcga acgccaagga cagacgccat gaaccacacc
     2281 attcccgaaa acctgaccgt cgccgtcgaa accaccgccc gcctgctcgg cgagatccgc
     2341 cgccagcgca acctgcgcca gcagatgagc gatccgtcca tgcgcacgaa cgccgccacg
     2401 cgccagccgc cggtgcccca ccacggcagc cccggcgaca cgcactgatt ccggctgatt
     2461 ccggctgatt ccggctgatt ccggctgatt ccggct
//
LOCUS       DAOQJH010000455         2495 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_30487_length_2495_cov_3.861885, whole genome shotgun sequence.
ACCESSION   DAOQJH010000455 DAOQJH010000000
VERSION     DAOQJH010000455.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2495)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30487_length_2495_cov_3.861885"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..830
                     /locus_tag="PLQ18_10240"
     CDS             <1..830
                     /locus_tag="PLQ18_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003277225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNE02075.1"
                     /translation="PPARLGSAVAASASAMQTAVITGPALAGFVYAAGPAAVFAASAL
                     LWALASLQLARIEPLNRPATSAPPPGLDTLFAGITFIRSRPVVLGAISLDLFAVLLGG
                     ATALLPIYARDILLTGPLGLGLLRSAPAIGALAMSLWLSRHPPQRGVGRLMFAAVAVF
                     GVATLVFALSTQFALSLAALVVLGASDMISVVIRASLVQLETPDEMRGRVSAVNSIFI
                     GASNQFGEFESGLTAALFGTVPAAALGGLGTLLVVLVWMRLFPDLRRRERLLETGTG"
     gene            986..1720
                     /gene="aqpZ"
                     /locus_tag="PLQ18_10245"
     CDS             986..1720
                     /gene="aqpZ"
                     /locus_tag="PLQ18_10245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014007892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HNE02076.1"
                     /translation="MHFSKRLGAEFIGTFWLVLGGCGSAVLAAAFPQVGIGLHGVALA
                     FGLTVLTMAYAIGHVSGCHLNPAVTVGLTVGGRFPAQEVVPYVVAQVLGATAAAFVLY
                     LIATGKAGASIGGFAANGYGELSPGKYGLGAALITEVVMTAMFLFVIMGATDARAPAG
                     FAPIAIGLCLTLIHLISIPVTNTSVNPARSTSQALFAGSEYLGQLWLFWLAPIVGGAI
                     GALVYRAVCSELKPAVAPVEDLRPAA"
     gene            1846..>2495
                     /locus_tag="PLQ18_10250"
     CDS             1846..>2495
                     /locus_tag="PLQ18_10250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012331932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HNE02077.1"
                     /translation="MSDARLRATLTGAVAILLWATLALGATLSAGLPPFELLALTFSI
                     GGLAGLAVVARGGRLARLRQPPAAFALGVAGLFGYHFFYFVALAHAPPVDASLINYLW
                     PLLIVLFSGLLPGERLQRHHLGGGLLGLAGAWVLISGGGTAPRLEYLPGYVSALACAF
                     VWSGYTVLNRRFAGLPTDGVAGQCLAVAVLAALCHAAWEPTVRPSAVQWAVIGWLGL"
BASE COUNT          246 a          943 c          882 g          424 t
ORIGIN      
        1 tgccgcccgc ccggctcggc agcgcggtcg cggcctcggc ctcggcgatg cagaccgccg
       61 tgatcaccgg cccggcgctg gccggtttcg tctacgccgc cgggccggca gcggtgttcg
      121 cggccagcgc gctgctgtgg gcgctcgcga gcctgcagct ggcgcgcatc gagccgctga
      181 accgcccggc caccagcgca ccgccgccgg ggctcgacac gctgttcgcc ggcatcacct
      241 tcatccgcag ccggccggtc gtgctcggcg cgatctcgct cgacctgttc gcggtgctgc
      301 tcggcggcgc gaccgcgctg ctgccgatct acgcgcgcga catcctgctg accggcccgc
      361 tcggcctcgg cctgctgcgc tcggcaccgg cgatcggcgc gctggcgatg tcgctgtggc
      421 tgtcgcgcca tccgccgcag cgcggcgtcg gccggctgat gttcgcggcc gtcgcggtgt
      481 tcggcgtcgc aacgctggtg ttcgcgctgt cgacgcagtt cgcgctgtcg ctggcggcgc
      541 tggtcgtgct cggggcctcg gacatgatca gcgtcgtgat ccgcgcctcg ctggtgcagc
      601 tcgaaacccc ggacgagatg cgcggccgcg tcagcgcggt caattcgatc ttcatcggcg
      661 cctcgaacca gttcggcgaa ttcgaatccg gactgaccgc ggcgctgttc ggcaccgtgc
      721 cggccgccgc gctcggcgga ctcggcacgc tgctcgtggt gctggtgtgg atgcggctgt
      781 tcccggacct gcgccggcgc gagcgcctgc tggaaaccgg caccggctga aagcccccga
      841 ggcggccccg ctgtccgaaa attaacctct gcgacggaat ggctctgcca tatcgggtac
      901 gccgtccgcc gggccccggt ggccggacgg cggcactccg tgcatctccc cccgatccgg
      961 ccattccccc gccaggaggt ttcccatgca tttcagcaag cgactcggtg ccgagttcat
     1021 cggcacgttc tggctcgtgc tcggcggttg cggcagtgcc gtgctggccg cggcgtttcc
     1081 gcaggtcggc atcggcctgc acggcgtggc gctcgccttc ggcctgaccg tgctgacgat
     1141 ggcctacgcg atcggccatg tttccggctg ccacctgaac ccggcggtca cggtcggcct
     1201 gaccgtcggc ggccgcttcc cggcgcagga ggtcgtgcct tatgtcgtcg cgcaggtgct
     1261 cggggcgacg gcagcggcct tcgtgctgta tctgatcgcc accggcaagg ccggcgcgtc
     1321 gatcggcggc ttcgcggcca acggctacgg cgagctgtcg cccggcaagt acggcctcgg
     1381 cgcggcgctg atcaccgagg tggtcatgac cgcgatgttc ctgttcgtga tcatgggcgc
     1441 gaccgatgca cgcgcaccgg ccggcttcgc gccgatcgcg atcggcctgt gcctgacgct
     1501 gatccacctg atcagcatcc cggtcaccaa cacctcggtg aacccggcgc gctcgacctc
     1561 gcaggccctg ttcgccggca gtgaatacct cggtcagctc tggctgttct ggctcgcgcc
     1621 gatcgtcggc ggcgccatcg gcgcactcgt ctaccgcgcg gtgtgcagcg agctgaagcc
     1681 ggccgtggca ccggtcgagg atctgcgccc ggccgcctga gccggcatct gcccctgccc
     1741 tgcccccggg cgcccgcgcg gcgcccgggg cgtctgcggc ggctggcatc cgcggcggcg
     1801 ctctggaagg atgcgcgacc cgtcccgccc ggagtatctg ccccgatgtc cgatgcccgc
     1861 ctgcgcgcca cgctgaccgg cgccgtcgcg atcctgctgt gggcgacgct cgcgctcggc
     1921 gcgacgctct ccgccggcct gccgccgttc gagctgctgg cgctgacctt ctcgatcggc
     1981 ggcctcgccg gcctcgccgt cgtcgcacgc ggcggccggc tcgcgcggct gcgccagccg
     2041 ccggcggcgt tcgcgctcgg cgtcgccggg ctgttcggct atcacttctt ctatttcgtc
     2101 gcactcgcgc acgcgccgcc ggtcgacgcc agcctgatca actacctgtg gccgctgctg
     2161 atcgtgctgt tctcgggcct gctgcccggc gagcgcctgc agcgccacca cctcggcggc
     2221 ggcctgctcg gcctcgccgg ggcctgggtg ctgatcagcg gcggcggcac ggcgccgagg
     2281 ctcgaatacc tgccgggcta tgtgtcggcg ctggcctgcg ccttcgtgtg gtcgggctac
     2341 acggtgctga accgccgctt cgccggcctg ccgaccgatg gcgtcgccgg ccagtgcctc
     2401 gcggtcgcgg tgctggcggc gctctgccac gcggcctggg agcccacggt ccggccctcg
     2461 gccgtgcagt gggcggtgat cggctggctc ggact
//
LOCUS       DAOQJH010000456         2492 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_30550_length_2492_cov_4.918753, whole genome shotgun sequence.
ACCESSION   DAOQJH010000456 DAOQJH010000000
VERSION     DAOQJH010000456.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2492
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30550_length_2492_cov_4.918753"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..753
                     /locus_tag="PLQ18_10255"
     CDS             <1..753
                     /locus_tag="PLQ18_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNE02078.1"
                     /translation="AAASALREQNRTLEARVAERTQALSQLASELAERNSELDRVRDA
                     IFLAFCSLAEARDNETGNHLLRTQHYVRVLALQLRRQPQYAGQLDDTAIELIFKSAPL
                     HDIGKVGIPDAILLKPGPLNAVETARMHEHPVIGRDAILMAERLLGAPLPFLRHAREI
                     AYSHHERWDGLGYPEGLAGEAIPLSARLMALADVYDALISRRVYKPPLPHATAVDMIL
                     ADRGRRFDPLVTDAFAAEAATFAGIAGRYRDE"
     assembly_gap    1191..1290
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1291..1627
                     /locus_tag="PLQ18_10260"
     CDS             <1291..1627
                     /locus_tag="PLQ18_10260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02079.1"
                     /translation="QPQPQPQQIPHLQGGKDMASEDAIEVVAKFGEEKDGSAWATLEY
                     YMENNPRQTEWRRIPGVVTGADKDDAMAKAEAIAVELSDLEIQQLQAAVRRLYEKGRM
                     IKRLEFPTT"
     gene            1673..1936
                     /locus_tag="PLQ18_10265"
     CDS             1673..1936
                     /locus_tag="PLQ18_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013457093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNE02080.1"
                     /translation="MTHIIAEPCIGCKDTACVDVCPVDCIYEHRNDSAAQSFIQPEEC
                     IDCGLCVDACPVEAIFPMEEVPDKWHRFIAMNYEQFRLEAPGI"
     gene            complement(2185..>2492)
                     /locus_tag="PLQ18_10270"
     CDS             complement(2185..>2492)
                     /locus_tag="PLQ18_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016403116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HNE02081.1"
                     /translation="NLSATFSPELSDLTLYVIDVAAGDKIPRKGGPGITRSDLLIINK
                     IDLAPHVGASLEVMDRDAKKMRGERPFVFTNMKTGEGLEAVIAFIEEKGMLDVALPA"
BASE COUNT          470 a          815 c          703 g          404 t
ORIGIN      
        1 gccgccgctt cggcactgcg cgagcagaac cgcacgctgg aggcgcgcgt cgccgagcgc
       61 acgcaggcac tgtcgcagct cgcctccgaa ctggccgagc gcaacagcga actcgaccgc
      121 gtgcgcgacg cgatcttcct ggccttctgc tcgctcgccg aagcccgcga caacgaaacc
      181 ggcaaccacc tgctgcgcac ccagcactac gtgcgcgtgc tggcgctgca gctgcgccgc
      241 cagccgcagt acgccggaca gctcgatgac acggcgatcg agctgatctt caagtccgcg
      301 ccgctgcacg acatcggcaa ggtcggcatc ccggacgcga tcctgctcaa gcccggcccg
      361 ctgaacgccg tcgagaccgc gcgcatgcac gagcacccgg tgatcggccg cgacgcgatc
      421 ctgatggccg aacgcctgct cggcgccccg ctgccgttcc tgcgccacgc gcgcgagatc
      481 gcctattcgc accacgagcg ctgggacggt ctcggctacc cggaagggct ggccggcgag
      541 gccatcccgc tgtcggcccg gctgatggcg cttgccgatg tctacgacgc gctgatcagc
      601 cgccgcgtct acaagccgcc cctgccgcac gccaccgccg tcgacatgat cctcgccgac
      661 cgcggccggc gcttcgaccc gctggtcacc gatgccttcg cggccgaggc cgcgacgttt
      721 gccgggatcg cggggcggta tcgggatgag tgagcgggat gcggcatccg cagtgccatt
      781 cgggtactgc cggccaggcc gcatgcccct gtcgtcgtga acgcaccgcc acgacagatt
      841 gcttacccgt cattcgccag acgcacgcat tcctgtccgg ccatgccaca tgaatggcat
      901 cgccatttca aatgaaattc gaatgaaacc cgcatgaaat ccggcttccg gttacggatc
      961 accagcaatg cttgacggat gcaccccgaa acccctgctt gccggaggct ttcacacgaa
     1021 ctccgccaac attaactttc cctcacccga ctgcggtttc tgcccgcgat tgcttggcca
     1081 tccgatggcc tgctgctagc ctcaaccccg cattccacat gcatgcgggg gctgaccgcg
     1141 gcattgcagc gattgcggca agcacccgca ggtcatgccc gtctgcaacc nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gcaaccgcaa ccgcaaccgc aacaaatccc
     1321 acaccttcag ggaggcaaag acatggccag tgaagatgca attgaagtgg tcgccaaatt
     1381 cggcgaagaa aaagacggca gcgcgtgggc gactctcgaa tactacatgg aaaacaatcc
     1441 gcgccagacc gaatggcgcc gcatccccgg ggtcgtcacg ggtgcagaca aggacgatgc
     1501 catggcgaaa gccgaggcga ttgccgttga actcagcgat ctcgaaatcc agcaactgca
     1561 ggcggccgtg cgcaggcttt atgaaaaagg aaggatgatc aagcgactcg aattcccgac
     1621 gacctgatcc accatcctct gattttcgca aggaatttca aggagtccga acatgacgca
     1681 catcattgcc gaaccctgca tcggctgcaa ggacaccgcc tgcgtggacg tctgcccggt
     1741 ggactgcatt tacgagcatc gcaacgattc ggccgcacag tctttcatcc agccggagga
     1801 atgcatcgac tgtggccttt gcgtcgatgc ctgtccggtc gaagccatct ttccgatgga
     1861 ggaagtgccg gacaaatggc accggttcat cgccatgaac tacgaacaat tcaggctgga
     1921 agcaccgggc atctgatacc gatcacatcg gaacgcttcg tttttcagcc cctcgcccct
     1981 tgcgggagag gggctggaaa gagggaaggc aagaaaaatc atgccctgac cggtgatcgg
     2041 catcgaccgg ttgcggtgcg ctcatggatg cgtaccgcgc cattgccgat tgccgtcacc
     2101 catgcgcatg aatgcgtccc ctgcccggct tctgctcaaa ggaaaccggg ctgcagccgt
     2161 caggccggct gaatgcaggc gccctcaggc cggcaacgcc acatccagca ttcccttctc
     2221 ctcgatgaac gcgatcacgg cctccagccc ctcgccggtc ttcatgttcg tgaacacgaa
     2281 cggccgctcg ccgcgcatct tcttcgcatc gcggtccatg acttccagcg aggcgccgac
     2341 gtgcggggcg aggtcgatct tgttgatgat cagcagatcc gagcgcgtga tgcccggtcc
     2401 gcccttgcgc ggaatcttgt cgccggcggc gacatcgatc acgtacagcg tcaggtcgga
     2461 aagctcgggg ctgaaggtgg ccgagaggtt gt
//
LOCUS       DAOQJH010000457         2488 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_30644_length_2488_cov_4.544595, whole genome shotgun sequence.
ACCESSION   DAOQJH010000457 DAOQJH010000000
VERSION     DAOQJH010000457.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2488)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2488)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2488
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30644_length_2488_cov_4.544595"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..581)
                     /gene="minD"
                     /locus_tag="PLQ18_10275"
     CDS             complement(<1..581)
                     /gene="minD"
                     /locus_tag="PLQ18_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006793462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HNE02082.1"
                     /translation="MAKIIVVTSGKGGVGKTTTSASFATGLALRGHKTVVIDFDVGLR
                     NLDLIMGCERRVVYDFVNVINNEATLNQALIKDKRVESLYVLPASQTRDKDALHKEGV
                     ERVLEELKQSFDYIICDSPAGIERGAMMAMYFADEALVVTNPEVSSVRDSDRILGILA
                     SKSRRAEQAEEPVREHLVLTRYSPERVEKGDMLS"
     gene            complement(593..1336)
                     /gene="minC"
                     /locus_tag="PLQ18_10280"
     CDS             complement(593..1336)
                     /gene="minC"
                     /locus_tag="PLQ18_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012702040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HNE02083.1"
                     /translation="MTQTPTAEAPFELKGSLFTLTVLHLRETDLGAIEQALLVKIAQA
                     PAFFANTPVVVDLDALADTAARPDFAGLARILRAHGLVPVGVRHGSAAQLAAAAAAGL
                     PMLPESRPALRREATTDAGGDAKPADPGPHNRVVVQPVRSGQQVYAADGDLTLLGPVS
                     AGAEVLADGSIHVYGPLRGRALAGVRGKLEARIFCLGLEAELVSVAGRYRILEKNGEG
                     WGKAVQIYLSGEHLIIEPLTVEAGAHRKS"
     gene            complement(1434..>2488)
                     /locus_tag="PLQ18_10285"
     CDS             complement(1434..>2488)
                     /locus_tag="PLQ18_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HNE02084.1"
                     /translation="DLPQPGAAELTDGYRTFLGLCSHEYFHAWNVKRIRPAAFTPYDY
                     QRENYTRLLWAFEGITSYYDDLMLRRSGLIDEAAWLQLLGETATRVWRGPGRHRQSVA
                     ESSFDAWTKFYRPDADTPNAVVSYYAKGALVALALDLTLRRLSRGTRSLDDLMRALWQ
                     RYGRPGLGVPEDGIESLAAELAGQPLADFFAACVHGTGDLPLAELLAGHGLVFRLRPA
                     EGATDKGGRAGSGTGGRVDAGLRTTLAGEELRVAHVLDGGPAQAAGLAAGDVLVALDG
                     LRVQPRGFERQLGRYAPGARVELLGFRRDELMRFPLELAAAPADTVWITPDPQADAEA
                     LQRRQAWLGETGVSPG"
BASE COUNT          350 a          873 c          898 g          367 t
ORIGIN      
        1 gacagcatgt cgcccttctc gacgcgctcg ggcgagtagc gcgtcagcac cagatgctcg
       61 cgcaccggct cctcggcctg ctcggcgcgg cgcgacttgc tggcgaggat gccgaggatg
      121 cggtcggaat cgcgcaccga cgacacctcg ggattggtga cgaccagcgc ctcgtcggcg
      181 aagtacatcg ccatcatcgc gccgcgctcg atgccggccg gcgagtcgca gatgatgtag
      241 tcgaagctct gcttgagctc ctcgagcacg cgctcgacgc cttccttgtg cagcgcatcc
      301 ttgtcgcgcg tctgcgaggc cggcagcacg tacaggctct cgacgcgctt gtccttgatc
      361 agcgcctggt tcagcgtcgc ctcgttgttg atgacgttga cgaagtcgta cacgacgcgc
      421 cgctcgcagc ccatgatcag gtcgaggttg cgcagaccca cgtcgaagtc gatgacgacg
      481 gtcttgtgcc cgcgcagcgc gagcccggtg gcaaagctcg cactggtcgt ggtcttgccc
      541 acgcccccct tcccggaggt gacgacgatg atcttggcca agggtgtgtc tcctaggact
      601 tgcggtgcgc gccggcttcg acggtgagcg gctcgatgat caggtgttcg ccggacaggt
      661 agatctgcac ggccttgccc cagccttcgc cgttcttttc gaggatgcgg tatcggccgg
      721 cgaccgacac cagctcggct tcgaggccga ggcagaagat gcgggcttca agcttgccgc
      781 gcacgccggc cagcgcccgg ccgcgcagcg gtccgtagac atggatgctg ccatcggcga
      841 gcacctcggc accggcactg accgggccga gcagcgtcag gtcgccgtcg gccgcgtaga
      901 cctgctggcc ggagcgcacc ggctgcacga cgacgcggtt gtgcgggccg gggtctgcgg
      961 gtttcgcatc gccgccggca tcggtcgttg cttcgcgccg cagcgccgga cggctttccg
     1021 gcagcatcgg cagcccggcg gcggcggcgg cggcgagctg cgcggcactg ccgtgacgca
     1081 cgccgaccgg caccagcccg tgggcgcgca ggatccgcgc cagcccggcg aaatccggcc
     1141 tcgccgccgt atcggcgagc gcgtcgagat cgacgacgac cggggtgttg gcgaagaacg
     1201 ccggggcctg ggcgatcttg acgagcagcg cctgctcgat cgcgccgaga tcggtctcgc
     1261 gcagatgcag cacagtcagc gtgaacaggc tgcccttgag ctcgaagggc gcctcggcgg
     1321 tcggcgtctg ggtcatggcg tcgggagcgt acgtcccctg tccggaattg ttgtgatctg
     1381 ttgcgggccg cagacggcgg tgactctaaa cactcgcccc cgaccgttca agctcacccc
     1441 ggcgacacgc cggtctcacc gagccaggcc tgacggcgct gcagggcttc ggcgtccgcc
     1501 tgcgggtcgg gcgtgatcca gacggtgtcg gccggggcgg cggcgagttc gagcgggaag
     1561 cgcatcagct cgtcgcgccg gaaccccagc agctcgacgc gcgcccccgg cgcgtagcgc
     1621 ccgagctggc gctcgaagcc gcgcggctgc acgcgcaggc cgtcgagggc gaccagcacg
     1681 tccccggccg cgagcccggc cgcctgcgcc ggcccgccgt cgagcacgtg cgcgacgcgc
     1741 agctcctcac cggcgagcgt cgtgcgcaag ccggcatcga cgcgcccgcc cgtgccgctg
     1801 ccggcccggc cgcccttgtc ggtggcgccc tcggccggac gcagccggaa cacgaggccg
     1861 tgtccggcga gcagctccgc gagcggcagg tcgccggtgc catgcacgca ggcggcgaag
     1921 aagtcggcga gcggctggcc ggcaagctcg gcggccagcg attcgatgcc gtcctcgggc
     1981 acgccgaggc cgggccggcc gtagcgctgc cacagcgcgc gcatcaggtc atcgagcgag
     2041 cgcgtgcccc ggctcaggcg gcgcagtgtc aggtcgaggg cgagcgccac cagcgcgccc
     2101 ttggcgtagt agctgaccac ggcgttcggg gtatcggcgt cggggcggta gaacttggtc
     2161 cacgcatcga agctcgactc cgccaccgac tggcgatgcc gccccggccc gcgccagacg
     2221 cgggtcgcgg tctcgccgag cagctgcagc caggcggcct cgtcgatcag gccggagcgc
     2281 ctgagcatca ggtcgtcgta atacgaggtg atgccctcga aggcccacag cagccgcgtg
     2341 tagttctcgc gctggtagtc gtacggcgtg aacgccgccg gccggatgcg cttgacgttc
     2401 caggcgtgga agtactcgtg gctgcacaga ccgagaaagg tccggtagcc gtcggtgagc
     2461 tcggcagccc ccggctgcgg caggtcgt
//
LOCUS       DAOQJH010000458         2486 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_30698_length_2486_cov_3.042781, whole genome shotgun sequence.
ACCESSION   DAOQJH010000458 DAOQJH010000000
VERSION     DAOQJH010000458.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2486)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2486)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2486
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30698_length_2486_cov_3.042781"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            337..897
                     /locus_tag="PLQ18_10290"
     CDS             337..897
                     /locus_tag="PLQ18_10290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02085.1"
                     /translation="MGRTIHVRSRHPLRAVAGLAVLLPALIGPVAPAAEAVPAARLAG
                     GALVLTAWRQARNRGSCAPQAFADTGEPAARPRRAYFSGGWAVAFDLPGRRSAFGIAG
                     TGTAAPGDSELAQWPQQLPLDGGLAGYGLSGRAPYPADDPDGFGRESLAYVRLDGQGC
                     LYNVWSQLGRAHLQRLLQQLRPVALP"
     gene            977..1441
                     /locus_tag="PLQ18_10295"
     CDS             977..1441
                     /locus_tag="PLQ18_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003605301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNE02086.1"
                     /translation="MSRPPLPPFTAETAAQKARMAEDAWNSRDPARVALAYTEDSLWR
                     NRAEFIRGREQIVAFLSRKWARELDYRLIKEVWAHAGERIAVRFAYEWHDDSGQWYRA
                     YGNENWEFDEAGLMRRRHASINDLPIAESARLFHWPAGRRPDGHPGLSELGL"
     gene            1581..2177
                     /locus_tag="PLQ18_10300"
     CDS             1581..2177
                     /locus_tag="PLQ18_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014745696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNE02087.1"
                     /translation="MPRLVTERADVLPLLAEVFREHGYEGASLQLISQATGLGKGSLY
                     HFFPGGKAEMAAAVLAEIDDWFERELYAPLRAAEDESVAAAIDAMFAAVDAYFRGGGR
                     VCLVGALALGAARDRFGAAIASYFARWVGALADALQRTGCDTTTATALAEATVAGIQG
                     ALVLTRALDDPAVFRRTLLALRERLPPGNPVDAGNAGP"
BASE COUNT          372 a          862 c          884 g          368 t
ORIGIN      
        1 gcgtgacgta gatcgccgac ggcacgatca gcgagcacag caccagcagc cagaaacgga
       61 tttcgacgaa cacggaatgc ggcatggcag gctcgctcgg aagacgggga gggcgcggac
      121 cgccgcgccg gcgaccgggc cgctgcggcg gtggcggccg gaagatcgcg gctcgcagcc
      181 gaggcagcgc agtgtgaggc aagttccggg gctgcggtca tgccccggag gccgtgatgg
      241 cccgcatgca accggaccat gacgggcgaa accggccggc ttgcgccagc gtcccctgcc
      301 atggttaccg ccatccgatc gcatcgaggg ctgtccatgg gcaggaccat ccacgtccgc
      361 agccgtcatc cgctccgcgc cgtcgccggt ctcgccgtgc tgctgccggc gctgatcggc
      421 ccggtcgcgc cggcggccga agcggtgccg gcggcgcggc tcgccggggg agcgctggtg
      481 ctgacagcct ggcgacaggc ccgcaaccgt ggcagctgcg caccgcaggc cttcgccgac
      541 accggcgagc ccgcggcccg gccgcggcgt gcgtattttt ccggcggctg ggccgtggcc
      601 ttcgacctgc ccggccggcg cagcgccttc ggcatcgccg gcaccggcac ggcggccccc
      661 ggcgacagcg aactcgcgca gtggccgcag cagctgccgc tcgatggcgg gcttgccggc
      721 tatgggctgt ccggccgcgc accgtatccg gcggacgatc cggacggctt cggtcgcgaa
      781 tcgctcgcct atgtgcgcct cgacggtcag ggctgtctgt acaacgtctg gagccagctc
      841 ggccgcgcgc atctgcagcg cctgctgcag cagttgcgac cggtggcctt gccgtagcgg
      901 cggcgcttgc ggaggagccg gcggtgcatg tacctttcgg tacagtctga aacgaccagt
      961 acagaaaggt gccgccatgt cccgtccgcc actgccgccg ttcaccgccg aaaccgctgc
     1021 gcagaaggcg cgcatggccg aggacgcctg gaacagccgc gatccggcgc gcgtcgcgct
     1081 cgcgtacacc gaggacagcc tctggcgcaa ccgcgccgaa ttcatccgcg gccgcgagca
     1141 gatcgtcgcc ttcctgagcc gcaagtgggc gcgcgaactc gactaccggc tgatcaagga
     1201 agtctgggcg catgccggcg agcgcatcgc cgtgcgcttt gcctacgaat ggcacgacga
     1261 ctccggccag tggtatcgcg cctacggcaa cgagaactgg gagttcgacg aagccggcct
     1321 gatgcgccgc cggcatgcct cgatcaacga cctgccgatc gccgaatccg cgcgcctgtt
     1381 ccactggccg gccggccggc ggcccgacgg gcatccgggc ctgagcgagc tggggctgtg
     1441 agacggatgc ggcactggca gcgctgtacg ggccgaagct cctgcccggc agcaccggca
     1501 tcgccgtttt tccacaccgg agaaagaagg gcgcaaggcc ggcaggctgc ggatgcgagc
     1561 ggattgcccg ggagaccccc atgccgcgcc tcgtgaccga acgcgccgac gtgctgccgc
     1621 tgctggccga agtgtttcgc gagcatggct acgaaggtgc gagcctgcag ttgatctcgc
     1681 aggccaccgg gctcggcaag ggcagccttt atcacttctt cccgggcggc aaggcggaga
     1741 tggccgcggc cgtgctggca gagatcgacg actggttcga gcgcgagctg tacgcaccgc
     1801 tgcgcgcggc cgaggacgag tctgtggccg cggcgatcga cgcgatgttc gcggccgtcg
     1861 atgcctattt ccgcggcggc gggcgcgtct gtctggtcgg cgcactggcc ctcggcgccg
     1921 cccgggatcg cttcggcgca gccatcgcca gctacttcgc ccgctgggtc ggcgcgctgg
     1981 ccgatgcact gcagcgcacc ggctgcgaca cgacgacggc caccgcgctg gccgaggcga
     2041 ccgtcgccgg catccagggt gcgctggtgc tgacgcgcgc cctcgacgac ccggcggtgt
     2101 tcaggcgcac gctgctggcg ttgcgcgaac gcttgccgcc gggtaatccg gttgatgccg
     2161 gcaatgccgg gccctgaagg tcacggcctt tttcctgccg gtccggcgcg ggtagacacg
     2221 gcctatccgt ccgatcgtcg gcggcctgac ggtcccgttg cctgacgctg cgcagggtcg
     2281 aacctgatgc aggcgctgtc tgcggagttc ggacgaatct ggccgctcga tcccggcccc
     2341 ggtacgatcc tgccagcctg taggctgggt tgaggaacga aacccagcat tgggaatggc
     2401 acgagaaagc agatgctcac gagcgaacaa atatgcaatt caaggctcca ccatctaatg
     2461 cgatggctgc tgggtttcct tcgtca
//
LOCUS       DAOQJH010000459         2479 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_30856_length_2479_cov_4.514026, whole genome shotgun sequence.
ACCESSION   DAOQJH010000459 DAOQJH010000000
VERSION     DAOQJH010000459.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2479)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2479)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2479
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30856_length_2479_cov_4.514026"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(165..416)
                     /locus_tag="PLQ18_10305"
     CDS             complement(165..416)
                     /locus_tag="PLQ18_10305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02088.1"
                     /translation="MRILLANLPADADAGAVTAFLAGHGMADATDVTLVPGSGSEPAA
                     TFELPVSAAVAETVALHLSGKAWQGQVLRATVFVTPAGD"
     gene            complement(544..>2479)
                     /locus_tag="PLQ18_10310"
     CDS             complement(544..>2479)
                     /locus_tag="PLQ18_10310"
                     /inference="COORDINATES: protein motif:HMM:NF022783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3141 domain-containing protein"
                     /protein_id="HNE02089.1"
                     /translation="VTIDARKRPYLIVDPRAGHGAGIGGFKPESQVGVALRAGHPVYF
                     VIFFRDPVPGQRLRDVAAAEARFLAEVVRRHPDSDKPCVIGNCQGGWAVMGMVAAHPD
                     AAGPLVINGAPMSYWAGTTGHNPMRYSGGMLGGSWLAQLTSDLAAGSFDGAWLVQNFE
                     NLDPAHNYWQKFYKLYAEVDTEASRFRDFEKWWGGYVTLTGSEIRAIVDNLFIGNRLA
                     AGEIPLDRGETLDLRRIRSPIVVFCSEGDNITPPQQALNWILDVYRDDATLRAFGQTI
                     VYLRHLKVGHLGVFVSGSVARKEYTEIVGTLDAIERLPPGLYEMLIDEDGRTPAGDPR
                     YRVELAQRSMSDIVALDTDSRREEDYFRVVAALSELNAKAYDLLASPVLQALAVPESV
                     ELQKMMHPLRAGHWAFSDWNPWLTALGPWVSWARSMRAPVEAGHPLRQLEQLWVDGIG
                     DLFDLYRDTRDMSVELTFYGLYGFLNVLGIPKPGERERSATSDAERVQREIAAWVDGH
                     IASGGYLEGYARMALLLFHAQGGIGRDGFVQVLERLDAMPEVAALDREARRRIVREQS
                     LIIAHAPERALATLAELLVTPGERERALAALDALFAGEALSDAAAALRKLLRSTFDSG
                     TSGPVRSGSPGRSRKSSPAA"
BASE COUNT          391 a          839 c          898 g          351 t
ORIGIN      
        1 cgcggatggt gcgggcgacg gcgtaggcga tcgaactctc gttggcgatg ccgaccacca
       61 ggccgcgcct gccgtgcagg ctgaagggat tgctcatcag tcgtgttcct catcccgttg
      121 tgatcctgac ccggccgggg ccgggccgct tgtggagggc gtgctcagtc cccggccggc
      181 gtcacgaaca cggtcgcgcg cagcacctgg ccctgccagg ccttgccgct caggtgcagg
      241 gcaacggtct cggcgaccgc ggcactgacc ggcagctcga aggtggcggc cggctccgag
      301 ccggagccgg gcacgagggt gacatcggtg gcatcggcca tgccgtgtcc ggcgagaaag
      361 gccgtgacgg ccccggcatc ggcgtcggcg ggaaggttgg caagcaggat gcgcatggtg
      421 cggactccgg aggaagccaa ggagcggaac gaccggcgcg cctgcgggcg cgccggtccg
      481 gctgacgaaa gcccgccggc ggaaaagacg gcgggctggc aagaaaaacg gaaaccggcg
      541 tgttcaggcg gccggtgacg atttgcggga acgtccggga gaacccgacc gtaccgggcc
      601 tgaggtcccg gaatcgaagg tgctgcgcag cagcttgcgc agcgcggccg ccgcatccga
      661 cagcgcctcg ccggcgaaca gcgcatcgag cgcggcgagg gcccgctcgc gctccccggg
      721 cgtgaccagc agttccgcca gcgtggccag cgcgcgctcg ggcgcgtgcg cgatgatcag
      781 gctctgctcg cgcacgatgc gccggcgcgc ctcgcgatcg agcgcggcga cctcgggcat
      841 cgcatcgagg cgctccagca cctgcacgaa gccgtcgcgg ccgatgcccc cctgcgcgtg
      901 gaacagcagc agcgccatgc gcgcgtagcc ttcgaggtat ccgccgctgg cgatatggcc
      961 atcgacccag gccgcgatct cgcgctgcac gcgctcggca tcgctggtgg cgctgcgctc
     1021 gcgctcgccc ggtttcggga tgccgagcac gttcaggaag ccgtagaggc cgtagaacgt
     1081 cagctcgacg ctcatgtcgc gcgtgtcgcg gtacaggtcg aacaggtcgc cgatgccgtc
     1141 gacccagagc tgttcgagct ggcgcagcgg atgtccggct tcgaccggtg cgcgcatcga
     1201 gcgcgcccag ctcacccacg ggccgagcgc cgtgagccac gggttccagt cgctgaaggc
     1261 ccagtgaccg gcgcgcagcg gatgcatcat cttctgcagc tcgacgctct cgggcacggc
     1321 cagcgcctgc agcaccggcg atgcgagcag gtcgtaggcc ttcgcgttca gctccgacag
     1381 cgcggcgacg acgcggaagt agtcctcctc gcggcgcgag tcggtgtcga gcgcgacgat
     1441 gtcgctcatc gaacgctgcg cgagttcgac gcgatagcgc ggatcgccgg ccggcgtgcg
     1501 gccgtcctcg tcgatcagca tctcgtagag gccgggcggc aggcgctcga tggcatcgag
     1561 cgtgccgacg atctcggtgt attccttgcg cgcgaccgaa ccggagacga acacgccgag
     1621 gtggccgacc ttcaggtggc gcagatagac gatggtctgg ccgaaggcac gcagcgtcgc
     1681 atcgtcgcgg tagacatcga ggatccagtt cagcgcctgc tgcggcggcg tgatgttgtc
     1741 gccctcggag cagaacacca cgatcggcga gcggatgcgg cgcaggtcca gggtctcgcc
     1801 gcgatcgagc ggaatctcgc cagccgcgag ccggttgccg atgaacaggt tgtcgacgat
     1861 ggcgcggatc tcggagccgg tcagcgtcac gtagccgccc caccacttct cgaagtcgcg
     1921 aaagcgcgag gcctcggtgt cgacctcggc gtagagcttg tagaacttct gccagtagtt
     1981 gtgcgccgga tcgaggttct cgaagttctg caccagccag gcgccgtcga agctgccggc
     2041 agccagatcg ctggtcagct gcgccagcca gctgccgccg agcatgccgc cgctgtagcg
     2101 catcgggttg tgaccggtcg tgccggccca gtacgacatc ggcgcgccgt tgatgaccag
     2161 cggcccggcg gcatcgggat gcgcggcaac catgcccatc accgcccagc cgccctggca
     2221 gttgccgatc acgcagggct tgtcgctgtc cggatggcgg cgcacgacct cggcgaggaa
     2281 gcgcgcctcg gcggcggcca cgtcgcgcag ccgctgaccc ggcaccggat cgcggaagaa
     2341 gatcacgaag tacaccggat ggccggcccg cagcgcgacg ccgacctgcg attcgggctt
     2401 gaagccgccg atgccggcac cgtggccggc acgcggatcg acgatcaggt acgggcgctt
     2461 gcgtgcatcg atcgtgacg
//
LOCUS       DAOQJH010000460         2478 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_30881_length_2478_cov_3.592654, whole genome shotgun sequence.
ACCESSION   DAOQJH010000460 DAOQJH010000000
VERSION     DAOQJH010000460.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2478)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2478)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2478
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30881_length_2478_cov_3.592654"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="PLQ18_10315"
     CDS             complement(<1..287)
                     /locus_tag="PLQ18_10315"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006020020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HNE02090.1"
                     /translation="MSSPWTPDSWRRHPAQQMPEYADAAALAAAEAQLAKYPPLVFAG
                     EARTLKKSLAEVAEGRAFVLQGGDCAESFAEFSANNIRDTFRVLLQMAVVLT"
     gene            492..917
                     /gene="dksA"
                     /locus_tag="PLQ18_10320"
     CDS             492..917
                     /gene="dksA"
                     /locus_tag="PLQ18_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012823400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HNE02091.1"
                     /translation="MTNELQLPGNYRPSADEPYMNERQLEYFRRKLLAWREELLAEAQ
                     STIDAMREAPTDGADEADRASRESDTSFELRTRDRYRKLLNKIESALKRIADGSYGYC
                     DETGEEIGLGRLEARPIATLTVEAQERRERRQSLQADPH"
     gene            1249..>2478
                     /locus_tag="PLQ18_10325"
     CDS             1249..>2478
                     /locus_tag="PLQ18_10325"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNE02092.1"
                     /translation="MNPALRTAEGHYTLAALDAFAWMLTPVWVFDLTVPTILWANAAA
                     LELWEARDLAELQARDFSDLTDTTRQRLARALAEIRDGRTCAEQWTFYPKGRPVHLIV
                     NLTGIVLSDGRLTTLHEARPVVPEQPDPLALRGIEALTHTPVMITLHDAEGRVLMQNP
                     AAQRSFGITDAAREQLRARCADPLDAASLLDALEPEAGWVGEIPRLTTAGLRWHRVKA
                     HRTTDPVTGAAAVLVSEHDVHELHEARTRLAHSEARLRSIVEGVAAVLWEVELPSGRL
                     SYVSPQIEQLSGYPSTEWYDPQVWLARIDAEDREHVRCSLSEQLAAGRDGTLEYRLQR
                     ADGSLAWWRTHFRVQRGLDGRPLRAIGFLLDISDRVATEERLRVAELVFDHADQGIFV
                     TDAEQRIRYVNPAFETIS"
BASE COUNT          389 a          917 c          831 g          341 t
ORIGIN      
        1 gtgagaacca ccgccatctg aaggagcacg cggaaggtgt cgcggatgtt gttggcggag
       61 aactcggcga agctctcggc acagtcaccg ccctgcagca cgaaggcgcg cccctcggcg
      121 acctcggcga gcgacttctt cagcgtgcgc gcctcgccgg cgaacaccag cggcgggtac
      181 ttcgcaagct gcgcctcggc ggcggcgagc gcggcggcat cggcgtattc gggcatctgc
      241 tgcgccggat gccggcgcca gctgtcaggg gtccacgggc tgctcacgct actcatgctg
      301 ctcacgccat tcatgccact catccacgtc gtctcgccgg tacgcaggcc ggcacgggca
      361 gggccggagc tgcaaacaag ccggcaagcc tcgcccatcc ttgtcaatcc cgcaagcgat
      421 ggtcatatca tgcggtctct ggcgcctgcc ctgccggccc gcctgcgcca gcccacagga
      481 gcccataact aatgaccaac gaactgcaac tgccgggcaa ttatcgtccg agcgccgacg
      541 agccctacat gaacgagcgc cagctcgaat acttccggcg caagctgctc gcctggcgtg
      601 aggaactgct cgccgaggcg cagtcgacga tcgacgcgat gcgcgaggct ccgaccgatg
      661 gcgccgacga ggccgaccgt gccagccgcg agagcgacac cagcttcgag ctgcgcacgc
      721 gcgatcgcta ccgcaagctg ctgaacaaga tcgaatcagc actgaagcgc atcgccgacg
      781 gcagctacgg ctactgcgac gagaccggcg aggaaatcgg tctcggccgg ctcgaagccc
      841 ggccgatcgc gacgctgacg gtcgaggcgc aggagcgccg cgagcgtcgc cagtcgctgc
      901 aggccgaccc gcactgaacg ccggacccgg ggacggctgg ctgacgctgc gcgaactcga
      961 cgcccgccac ggcgccggca agggcacggc gttccgggcg tttcgcgccg cgctgcccgg
     1021 cctcgtcgaa ggccgtgact tccgccggct cgatgccgtc gccgatgcgg ccgaaatcgc
     1081 cgtcctgcgc gctggcgggc ggacctacgc ctccagcgtc cacatcgtgc tgctcagtcc
     1141 ggcgacggcc gcacggctgc tgacgcactg aaagcccgtg cccggtgcac atccgctgcg
     1201 agcggattat cgtgtgcggt cgtccccgtc ctgcccggat cgcccgccgt gaaccccgcc
     1261 ttgcgcaccg ccgaaggcca ttacacgctg gccgcgctcg atgcattcgc ctggatgctg
     1321 acgccggtct gggtgttcga cctgacggtg ccgaccatcc tgtgggccaa cgccgcggcg
     1381 ctcgaactct gggaggcacg cgatctcgcc gagctgcagg cgcgcgactt ctccgatctc
     1441 accgacacga cgcgccagcg cctggcccgc gcgctggccg aaatccgcga cgggcgcacc
     1501 tgcgccgagc agtggacctt ctatccgaag ggccgcccgg tgcacctgat cgtcaacctg
     1561 accggcatcg tgctctccga cggccggctg acgacgctgc acgaggcccg gccggtcgtg
     1621 cccgaacagc ccgatccgct ggcgctgcgc ggcatcgagg ccctcacgca cacgccggtg
     1681 atgatcacgc tgcacgacgc agaaggccgc gtgctgatgc agaacccggc cgcgcagcgc
     1741 agcttcggca tcaccgatgc ggcccgcgag cagttgcgcg cgcgctgcgc cgacccgctc
     1801 gacgccgcct cgctgctgga cgcactcgaa cccgaagccg gctgggtcgg ggaaatcccg
     1861 cgcctgacca ccgccggcct gcgctggcat cgcgtcaagg cgcatcgcac gaccgacccg
     1921 gtcaccgggg cggcggccgt gctggtctcc gagcacgacg tgcatgagct gcacgaggcg
     1981 cgcacgcggc tcgcgcacag cgaggcgcgg ctgcgctcga tcgtcgaagg cgtggccgcc
     2041 gtgctctggg aggtcgagct gccgtccggg cggctgtcgt acgtgtcgcc gcagatcgag
     2101 cagctctcgg gctatccgag caccgagtgg tatgacccgc aggtctggct cgcgcgcatc
     2161 gacgccgagg accgcgagca cgtgcgctgc agcctgagcg aacagctggc ggccgggcgc
     2221 gacggcacgc tcgaataccg cctgcagcgc gccgacggca gcctcgcgtg gtggcgcacg
     2281 cactttcgcg tgcagcgcgg cctcgacggc cggccgctgc gggcgatcgg cttcctgctc
     2341 gacatcagcg accgcgtcgc caccgaggag cggctgcgcg tcgccgaact ggtgttcgat
     2401 cacgccgatc agggcatctt cgtcaccgac gccgaacagc gcatccgcta cgtgaacccg
     2461 gccttcgaga cgatctcc
//
LOCUS       DAOQJH010000461         2477 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_30916_length_2477_cov_3.317919, whole genome shotgun sequence.
ACCESSION   DAOQJH010000461 DAOQJH010000000
VERSION     DAOQJH010000461.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2477)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2477)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2477
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30916_length_2477_cov_3.317919"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="PLQ18_10330"
     CDS             complement(<1..209)
                     /locus_tag="PLQ18_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HNE02093.1"
                     /translation="MRRNPDAARYRANLQGEIDSAALYRAMADSAGDAAQAELYRRLA
                     AVEDAHAAFWQRRLKAVGVDTKRERP"
     gene            complement(289..711)
                     /locus_tag="PLQ18_10335"
     CDS             complement(289..711)
                     /locus_tag="PLQ18_10335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010658490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HNE02094.1"
                     /translation="MNVLYRTEATATGGRTGTARTADGAFEVRLVTPKELGGPGGSGN
                     NPEQLFAAGYSACFLGAIKFVAGQKKVKIAEDSTVTAQVGIGPRDDGQGFGLDVALSI
                     DLPGLDRETAEAIVQQAHIVCPYSHATRGSLDVRLSLK"
     gene            complement(834..1550)
                     /locus_tag="PLQ18_10340"
     CDS             complement(834..1550)
                     /locus_tag="PLQ18_10340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017255830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HNE02095.1"
                     /translation="MTAILRKAGDRGVAEHGWLSSRHTFSFGHYDDPRHRGFSDLLVI
                     NDDRVAPGMGFGQHPHRDMEIFSYVLDGALEHRDTLGNGSVIRPGDVQMISAGRGIAH
                     SEFNHSRSDEVHFLQIWIVPEVRGIAPQYQQQHFDAASKRGRLRVILSPDGREGSLKL
                     HQDAVVHAGLFADDETASLAIAAGRVVYVHVARGSVQVNGVALQAGDGLKLIDAGAVN
                     ISDGQDAEVLVFDLRPETRH"
     gene            complement(1749..2234)
                     /locus_tag="PLQ18_10345"
     CDS             complement(1749..2234)
                     /locus_tag="PLQ18_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020163997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HNE02096.1"
                     /translation="MSTNTYAAASVTTAATTVTTAATGAVNRYAPAAGRVLLGGLFLV
                     SGLGKLAAPAATIGYITAAGLPAPELAYAGALAVEIGLAGALIAGYRTQLVALLMAVF
                     TLVTAFAFHFQPADQGQVIHFLKNLAITGGLLQVAAFGAGAFSLDALRSRRLRPVIAA
                     A"
     gene            2377..>2477
                     /locus_tag="PLQ18_10350"
     CDS             2377..>2477
                     /locus_tag="PLQ18_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003191903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNE02097.1"
                     /translation="MNALEDMQLFATTVEAGSFTAAAERLGLSKQFV"
BASE COUNT          352 a          817 c          933 g          375 t
ORIGIN      
        1 gggcgctcgc gcttcgtatc gacgccgacc gccttcagcc gccgctgcca gaacgccgcg
       61 tgcgcgtcct cgaccgccgc gaggcgccgg tacagctcgg cctgcgcagc atcgccggcg
      121 ctgtcggcca tcgcccggta cagcgcggcg ctgtcgatct cgccctgcag gttggcgcgg
      181 tagcgggcgg catcggggtt gcgacgcatg gcgggcgtcc tcgcagcggg cggatcacgg
      241 atgacagagg ccgcccgcac gcgggcaagg tgcgggcggc cggatcactc acttgaggct
      301 caggcgcacg tcaaggctgc cgcgggtggc gtgcgagtac ggacagacga tgtgcgcctg
      361 ctgcacgatg gcctcggcgg tctcgcgatc gaggccgggc aggtcgatgc tcagcgccac
      421 gtcgaggccg aagccctggc cgtcgtcgcg cgggccgatg ccgacctggg cggtgacggt
      481 gctgtcctcg gcgatcttca ccttcttctg gccggcgacg aacttgatcg cgccgaggaa
      541 gcaggccgag tagccggcag cgaacagctg ctcggggttg ttgccgctgc cgccggggcc
      601 gccgagttcc ttcggcgtga ccagccggac ttcgaaggcg ccgtcagcgg tgcgggcggt
      661 gccggtacga ccgccggtgg cggtggcttc ggtgcggtac aggacgttca tggcggtgct
      721 cctcgcagac ttcgcggggg ggcgatgccc gccacgcagg cgggcatccg ggggtcgggg
      781 gtggagggaa tcggccggtg cgccgctcgc ggacggcgca ccggtcacgg ccttcagtgc
      841 cgggtttcgg ggcgcaggtc gaagaccagc acctcggcgt cctgcccgtc gctgatgttc
      901 acggccccgg catcgatgag cttcaggccg tcgccggcct gcagcgcgac accgttgacc
      961 tgcacgctgc cgcgggcgac gtgaacgtag acgacgcgac cggcggcaat cgccaggctt
     1021 gccgtttcgt catccgcgaa cagcccggcg tggacgaccg cgtcctgatg cagcttcagc
     1081 gaaccctcgc gcccgtccgg cgacaggatc acccgcagcc ttccgcgctt gctcgccgca
     1141 tcgaagtgct gctgctggta ctgcggcgcg atgccgcgca cctcgggcac gatccagatc
     1201 tgcaggaagt gcacctcgtc gctgcgcgag tggttgaact cgctgtgcgc gatgccgcgc
     1261 ccggcgctga tcatctgcac gtcgccgggg cggatcaccg agccgttgcc gagcgtgtcg
     1321 cggtgttcga gcgcgccgtc gagcacgtag gaaaagatct ccatgtcgcg gtgcgggtgc
     1381 tggccgaagc ccatgcccgg cgcaacgcga tcgtcgttga tgaccagcag gtccgagaag
     1441 ccgcgatggc gcggatcgtc gtagtggccg aacgagaagg tgtggcgcga gctgagccag
     1501 ccgtgctcgg cgacgccgcg gtcaccggcc ttgcgcagga ttgcagtcat ggtcttgctc
     1561 ccgttgcttc ggaggcggac cggcgcgggg gggcgccggg gccgggggtt caggattcag
     1621 gcgtcgtcca tggcgggcgg atgcggccgg ggtgccgggg gtttcccctc tcccccaacc
     1681 cctcccccgc gaggggggga ggggcttgaa ggcaggcacc tgcgatccgc tcagccggat
     1741 caggtgtttc aggccgcggc gatgaccggg cgcaggcgac ggctgcgcag tgcatcgagg
     1801 ctgaaggcgc cggcgccgaa ggccgcgacc tgcagcaggc cgccggtgat cgccagattc
     1861 ttcaggaagt ggatcacctg gccctggtcg gcgggctgga agtggaaggc gaaggccgtg
     1921 accagcgtga acaccgccat cagcagcgcc accagttgcg tgcggtaacc ggcgatcagc
     1981 gcacccgcca gcccgatctc gaccgccagc gcaccggcgt aggccagttc cggcgccggc
     2041 aggccggcgg cggtgatgta gccgatcgtg gccgccggcg cggcgagctt gccgaggccg
     2101 ctgaccagga acagtccgcc gagcagcacg cggccggcgg ccggggcgta gcggttgacg
     2161 gcgccggtcg cagcggtggt gaccgtggtg gcagcagtgg tgacggaagc ggcggcgtag
     2221 gtattggtgc tcatggcgat gacctcttga gtgcgggttt ccggtttcgg tggctgccgg
     2281 tctggccggt gccttgccga tggagcgcac tctacgcagc cacgatcacg ccataaatca
     2341 gccagaatgc gtcacatcgt ccactggaga aggacaatga acgcgctcga agacatgcag
     2401 ttgttcgcca ccacggtcga ggccggcagc ttcacggccg cggccgaacg cctcgggctg
     2461 tcgaagcagt tcgtcag
//
LOCUS       DAOQJH010000462         2475 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_30954_length_2475_cov_3.465289, whole genome shotgun sequence.
ACCESSION   DAOQJH010000462 DAOQJH010000000
VERSION     DAOQJH010000462.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2475)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2475)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2475
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30954_length_2475_cov_3.465289"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1561
                     /locus_tag="PLQ18_10355"
     CDS             <1..1561
                     /locus_tag="PLQ18_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013258603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNE02098.1"
                     /translation="YVLLLGVLGVLFLRLPTSFLPDEDQGTLYAQITLPVGATQERAL
                     DVIAQVENYFLDHEKDAVRSVFAVSGFGFGGNGQNVATAFIKLRDWSLRDRDELRAPA
                     IVERATKTLGRIRDAQVFVMNPPQIRGLGNSTGFDVQMQDSGGVGHEALVAARERFLE
                     LARQEPLLTRVRPQGLDDTPQLQVDIDTAKAGALGLTMSEVNDALGTALGGDYVNDYL
                     DRGRVKKVYVQGDAPFRMRPEDISRWYVRNGDGAMVPFSDFSHAYWVYGSPRLERFNG
                     SSSIEIVGEAAPGRSSGEAMAAVERIMGQMPQGIAYEWTGLSRQERQSGSQAPYLYAV
                     SVLVVFLALAALYESWSIPFAVLLVVPLGVIGAVTATELRGLENDVYFQVGLLTTIGL
                     SAKNAILIVEFAHELQRKGMGLIEATLSAAHQRLRPIVMTSAAFMLGVLPLAISTGAG
                     SGSRHSIGTGVLGGMLSATVLALFFVPLFFVLVRRYLGGRERAQAQAGIPADPAASPA
                     LPAHPSTGDAR"
     gene            1558..>2475
                     /locus_tag="PLQ18_10360"
     CDS             1558..>2475
                     /locus_tag="PLQ18_10360"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HNE02099.1"
                     /translation="MKRLSPPAVALAAVLASGCMNLAPDYVRPSLPVADAWPAGTKPP
                     VAQPLPAPVWRDYYRDPRLRQLIETALADNRDLRLAALDIDKARALYRVQAAESLPQV
                     NAGLSQSVQRTPGQLSQSGRATVSRQYTAQLGVSAWELDLWGRLKNLETQALESYFSS
                     AEARRATQLTLIAEVANAWLTLAADRERLALARETLASQQASYRLTERTVSSGIGSGL
                     DLAQARTSVETARGDVATYSAQVEQDRNALRLLLGSEPPENLLPDKLPADAATLAELP
                     VGLPSSVLLQRPDILAAEHTLIGANASIGA"
BASE COUNT          345 a          887 c          884 g          359 t
ORIGIN      
        1 gtacgtgctg ctgctcggcg tgctcggcgt gctgttcctg cgcctgccga cgagcttcct
       61 gcccgatgag gaccagggca cgctgtacgc gcagatcacg ctgccggtcg gggcgacgca
      121 ggagcgcgcg ctcgacgtga tcgcccaggt cgagaactac ttcctcgacc acgagaagga
      181 cgccgtgcgc tcggtgttcg cggtttccgg cttcggcttc ggcggcaacg gccagaacgt
      241 cgcgaccgcc ttcatcaagc tgcgcgactg gagcctgcgc gaccgtgacg aactgcgcgc
      301 cccggcgatc gtcgagcgcg cgacgaagac gctcgggcgc atccgcgatg cgcaggtgtt
      361 cgtgatgaac ccgccgcaga tccgcggcct cggcaactcg accggcttcg acgtgcagat
      421 gcaggacagc ggcggcgtcg gtcacgaggc gctggtggcg gcgcgcgagc gcttcctcga
      481 actcgcacgc caggagccgc tgctgacgcg cgtgcgcccg cagggcctcg atgacacgcc
      541 gcagctgcag gtcgacatcg acaccgccaa ggccggcgcg ctcgggctga cgatgagcga
      601 ggtcaacgac gcgctcggca ccgcactcgg cggcgactac gtcaacgact acctcgaccg
      661 cggccgcgtc aagaaggtct acgtgcaggg cgacgcgccg ttccggatgc ggcccgagga
      721 catcagccgc tggtacgtgc gcaacggcga cggtgcgatg gtgccgttct cggacttctc
      781 gcatgcgtac tgggtctacg gctcgccgcg cctggagcgc ttcaacggca gctcgtcgat
      841 cgagatcgtc ggcgaggccg cgccggggcg cagttccggc gaggcgatgg cggcggtcga
      901 gcggatcatg ggccagatgc cgcagggcat cgcctacgaa tggaccggcc tgtcgcggca
      961 ggagcgccag tccggctcgc aggcgccgta tctgtacgcg gtgtcggtgc tcgtggtgtt
     1021 cctggcgctg gcggcgctgt acgagagctg gtcgatcccg ttcgcggtgc tgctggtcgt
     1081 gccgctcggc gtcatcggcg cggtcacggc caccgagctg cgcgggctgg agaacgacgt
     1141 gtacttccag gtcgggctgc tgacgacgat cggcctgtcg gcgaagaacg ccatcctgat
     1201 cgtcgagttc gcgcatgagc tgcagcgcaa gggcatgggc ctgatcgagg cgacgctgtc
     1261 ggccgcgcac cagcgcctgc ggccgatcgt gatgacctcg gcggcgttca tgctcggcgt
     1321 gctgccgctc gcgatcagca ccggcgccgg ttccggcagc cggcattcga tcggcaccgg
     1381 cgtgctcggc ggcatgctgt cggcaaccgt gctggcgctg ttcttcgtgc cgctgttctt
     1441 cgtgctggtg cgccgctacc tcggcgggcg cgagcgtgcg caagcgcagg ccggcattcc
     1501 tgccgatcct gccgcttccc cggcgctgcc ggcacacccg agcaccggag acgcccgatg
     1561 aagcgcctga gcccgcctgc cgtcgcgctg gccgccgtgc tcgcgagcgg ctgcatgaac
     1621 ctcgcccccg actacgtgcg tccgtcgctg ccggtcgccg atgcctggcc ggccggcacg
     1681 aaaccgccgg tggcacagcc gctgccggcc ccggtctggc gcgattacta ccgcgatccg
     1741 cgcctgcgcc agctgatcga gaccgcgctc gccgacaacc gcgacctgcg tctggcggcg
     1801 ctcgacatcg acaaggcgcg cgcgctgtac cgcgtgcagg ccgccgaatc gctgccgcag
     1861 gtgaacgccg gcctgtcgca gagcgtgcag cgcacgcccg ggcagctgtc gcagagcggc
     1921 cgggcgacgg tgtcgcgcca gtacacggcg cagctcggcg tcagcgcctg ggagctcgat
     1981 ctgtgggggc ggctgaagaa cctcgaaacc caggcgctgg aaagctactt ctccagcgcc
     2041 gaggcgcgac gcgcgacgca gctgacgctg atcgccgagg tcgccaatgc ctggctgacg
     2101 ctcgccgccg accgcgagcg gctggcgctg gcgcgcgaga cgctagccag ccagcaggcc
     2161 tcgtaccggc tgaccgagcg cacggtcagc tccggcatcg gctcggggct cgatctggcg
     2221 caggcgcgca ccagcgtcga gaccgcgcgc ggcgatgtcg ccacctactc ggcgcaggtc
     2281 gagcaggacc gcaacgcgct gcgcctgctg ctcggcagcg agccgccgga aaacctgctg
     2341 ccggacaagc tgccggccga tgccgcgacg ctcgccgagc tgccggtcgg cctgccgtcc
     2401 tcggtgctgc tgcagcgccc cgacatcctc gccgccgagc acacgctgat cggcgccaac
     2461 gccagcatcg gcgcc
//
LOCUS       DAOQJH010000463         2473 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_30985_length_2473_cov_4.749380, whole genome shotgun sequence.
ACCESSION   DAOQJH010000463 DAOQJH010000000
VERSION     DAOQJH010000463.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2473)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2473)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2473
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30985_length_2473_cov_4.749380"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..461
                     /locus_tag="PLQ18_10365"
     CDS             <1..461
                     /locus_tag="PLQ18_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005792988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNE02100.1"
                     /translation="PVAAVIGRPEVMDAPNPGGLGGTYAGNPVACAAARAVLDVFADE
                     HLLDRARQLGEHLRARLEALKTRFASIAEVRGLGPMVAFELCTDADPARPAAELTKAL
                     TALAAERGLILLACGVYGNVIRILVPLTAEPAIVDEGLDILAGCLETLGA"
     gene            663..>2473
                     /locus_tag="PLQ18_10370"
     CDS             663..>2473
                     /locus_tag="PLQ18_10370"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNE02101.1"
                     /translation="MKSKVASALPQLLLALLVLAIVASLGVAGLRLRERLNAPPPDTP
                     NAAHALASTTERALLRVALTIGRLQTGEAPVALREAHAQLAAVLTEAGASTPAASQLL
                     ALPDAAAAWGEARGLADELAPLVKLPQLDAPLLAGRFVPRLDATAARIGKVADAADAA
                     SRQPDPDHNADLRALIALALAGLGGLLLAGTLAHRQSQLARLREQVAHGIAERTVGQQ
                     QTSERLVTLSHAIEQSPAIVLIADASGCVEYANPRFETVTGYPVSEILGKRVGTLPTD
                     RLDADERAQMWAELSAGKIWQRESESRTRRDDPYWERTQIAPVRDLQGELTHFVVVKE
                     DVSERKQFEAKLLRQARYDELTGLPNRLLAMERLTAEIDRSDRADGAISALLFVDVQG
                     LKRVNDSYGHSFGDRLLVAIGMRLLERIGRHDMVARFGGDEFLLLLARRSDVGAVRRF
                     AKELVKAFTSAIDIQGQQVHTSITVGGALAPLDGRDADTLLRCADTARSHANAEGGST
                     CRFYDESMRRRALETAQLDNLLRGALERNEFSLAYQPVVDLASGRPIGAEALLRWNSP
                     VLGPVSPDRFIQLAEDTGLIVPIGEWVLEQACRELAA"
BASE COUNT          348 a          939 c          852 g          334 t
ORIGIN      
        1 atccggtcgc ggccgtcatc ggccggcccg aggtcatgga cgcgccgaac cccggcggtc
       61 tcggcggcac ctacgccggc aacccggtgg cctgtgccgc ggcgcgcgcg gtgctcgacg
      121 tattcgccga cgagcacctg ctcgatcgcg cccgccagct cggcgagcac ctgcgcgccc
      181 ggctcgaagc gctgaagacg cgcttcgcga gcatcgccga ggtgcgcggc ctcgggccga
      241 tggtcgcctt cgaactctgc accgacgccg atccggcgcg cccggccgcc gagctgacga
      301 aggcgctgac ggcgctcgcc gccgagcgcg ggctgatcct gctggcctgc ggcgtctacg
      361 gcaacgtcat ccgcatcctc gtgccgctga ccgccgaacc ggcgatcgtc gacgagggcc
      421 tggacatcct cgccggctgt ctggagacgc tcggcgcctg agccgctccc ccgcaccggc
      481 cggccccgtc tccccgaccc ggccgaccgg tgcggcatct tccgcaaagc agcattgatc
      541 gaggtcgtta cagactcatt cgtcgctgtc ccgcccattt tccttatgga taatggccgt
      601 tcgcccatca cctccttcac ataaaggagc cgccgggccg gcggcagtcg ggagacgtgc
      661 tcatgaagtc caaggtcgcc tccgccctgc cgcaactgct gcttgcgctg ctggtgctcg
      721 ccatcgtcgc cagcctcggc gtcgccggcc tgcgcctgcg cgaacgactg aacgccccgc
      781 cccccgatac gccgaacgcc gcacacgcac tggcgagcac gaccgagcgc gcgctgctgc
      841 gcgtcgcgct gacgatcggc cggctgcaga ccggcgaggc accggtcgcg ctgcgcgagg
      901 ctcacgcgca gctcgcggcc gtgctcaccg aggccggcgc cagcaccccg gcggcgagcc
      961 agctgctggc gctgccggac gccgccgccg cctggggcga ggcgcgcgga ctcgccgatg
     1021 aactcgcacc gctggtgaag ctgccgcagc tcgacgcgcc gctgctcgcc ggacgcttcg
     1081 tgccgcggct cgacgccacc gccgcgcgca tcggcaaggt ggccgatgcc gccgacgccg
     1141 ccagccgcca gcccgacccg gaccacaacg ccgacctgcg cgccctgatc gcgctcgccc
     1201 tcgccggtct gggcgggctg ctgctcgccg gcacgctggc gcatcgccag tcgcagctcg
     1261 cgcggctgcg cgagcaggtc gcgcacggca tcgccgaacg caccgtcggc cagcagcaga
     1321 cctccgagcg gctggtgacg ctgtcgcacg cgatcgagca gagcccggcc atcgtgctga
     1381 tcgccgatgc cagcggctgc gtcgaatacg ccaatccgcg cttcgagacc gtgaccggct
     1441 atccggtcag cgagatcctc ggcaagcgcg tcggcacgct gccgaccgac cggctcgatg
     1501 ccgacgagcg cgcgcagatg tgggccgagc tgtcggccgg caagatctgg cagcgcgaga
     1561 gcgaatcgcg cacgcgccgc gacgatccgt actgggagcg cacgcagatc gcgccggtgc
     1621 gcgatctgca gggcgagctg acgcacttcg tcgtggtcaa ggaggatgtc agcgagcgca
     1681 agcagttcga ggcgaagctc ctgcgtcagg cgcgctacga cgaactgacc ggcctgccga
     1741 accggctgct ggcgatggag cgcctcaccg ccgagatcga ccgctccgac cgggccgacg
     1801 gcgcgatctc ggcgttgctg ttcgtcgacg tgcagggtct caagcgcgtc aacgacagct
     1861 acggccacag cttcggcgac cgcctgctgg tggcgatcgg catgcgcctg ctcgaacgca
     1921 tcggccgcca cgacatggtc gcgcgcttcg gcggcgacga gttcctgctg ctgctggcac
     1981 ggcgcagcga cgtgggtgcc gtgcgccgct tcgcgaagga gctggtcaag gccttcacgt
     2041 cggcgatcga catccagggc cagcaggtgc acacctcgat cacggtcggc ggcgcgctgg
     2101 cgccgctcga cggccgcgat gccgacacgc tgctgcgctg cgccgacacc gcgcgcagcc
     2161 acgccaatgc cgagggcggc agcacctgcc gcttctacga cgagagcatg cgccggcgcg
     2221 cgctggagac cgcgcagctc gacaacctgc tgcgcggggc gctggagcgc aacgagttct
     2281 cgctcgcgta ccagccggtc gtcgatctcg ccagcggccg gccgatcggc gccgaggcgc
     2341 tgctgcgctg gaacagcccg gtgctcggcc cggtgtcgcc ggatcgcttc atccagctcg
     2401 ccgaggacac cggcctgatc gtgccgatcg gcgaatgggt gctcgaacag gcctgccgtg
     2461 aactggcggc ctg
//
LOCUS       DAOQJH010000464         2470 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_31037_length_2470_cov_4.077847, whole genome shotgun sequence.
ACCESSION   DAOQJH010000464 DAOQJH010000000
VERSION     DAOQJH010000464.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2470)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2470)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2470
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31037_length_2470_cov_4.077847"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..591)
                     /gene="lolA"
                     /locus_tag="PLQ18_10375"
     CDS             complement(<1..591)
                     /gene="lolA"
                     /locus_tag="PLQ18_10375"
                     /inference="COORDINATES: protein motif:HMM:TIGR00547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HNE02102.1"
                     /translation="MSLLSRLPAGLLLACALLLSVPARAEPSVTQYFHDLQSLQAGFT
                     QRVSDAQGRIVQESAGSMAMQKPGRFRWDYTAPSPQTIVADGQRLWVHDPGLDQVTVR
                     PLDAALGGTPLALLSGAAPIEDVFKVGPVQNRGGLSWYLLTPKDDGSDFKALRVGFRG
                     DTLAALELEDALGGRTRVEFSHLQRNGRVDPAQFRFT"
     gene            complement(825..>2470)
                     /locus_tag="PLQ18_10380"
     CDS             complement(825..>2470)
                     /locus_tag="PLQ18_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007162302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HNE02103.1"
                     /translation="PLPAPAAAAPVAVPAAAPRPQPPARAEGVSEAAAALFGLRPKRE
                     VLDSLPPLELLDLPREQAAGYSPEALDGMSRQVESKLAEFGVDVTVVNVEPGPVVTRF
                     ELDLAPGVKVSQISNLAKDVARGLSVVSVRVVEVIPGKSVIGLEIPNRRRQIVSLREI
                     IAAPVWQGAGSPLTVALGHDIGGRPVVGNLAKMPHVLVAGTTGSGKSVAVNCMLISML
                     YKAGAEQLRLILIDPKMLELSIYEGIPHLLAPVVTDMKDAANALRWCVAEMERRYRLM
                     AALRVRNIAGFNKQVSEAIELGEPIPDPLWKNEDSLDPERPLLEPLPYVVVVIDELAD
                     MMMIVGKKVEELIARLAQKARAAGIHLILATQRPSVDVITGLIKANIPTRIALTVASK
                     IDSRTILDQMGAEALLGHGDMLYLPPGTGFPQRVHGAFVDDHEVNAVVEYLKQTGEPD
                     YVTGILDEPVDGAAFPGDPLAGGGGDSDGDSDPLYDQAVRIVVESRRASVSGVQRRLK
                     IGYNRAARLVEEMERAGIVGPIQSNGSREVLVGGAQADA"
BASE COUNT          371 a          865 c          866 g          368 t
ORIGIN      
        1 cgtgaagcgg aactgcgccg gatcgacgcg gccgttgcgc tgcagatggc tgaactcgac
       61 gcgcgtgcgc ccgccgaggg catcctccag ttcgagcgcg gccagcgtgt cgccgcggaa
      121 accgactcgc agcgccttga agtcgctgcc gtcgtccttc ggcgtcagca gataccagct
      181 caggccgccg cggttctgca ccggcccgac cttgaacacg tcctcgatcg gtgcggcccc
      241 cgacagcagc gccagcggtg tgccgccgag cgcggcatcg agcgggcgca cggtgacctg
      301 gtcgaggccg gggtcatgca cccacaggcg ctgaccatcg gcgacgatgg tctgcgggct
      361 cggcgccgtg taatcccagc ggaagcgccc gggcttctgc atcgccatcg aaccggccga
      421 ttcctgcacg atccgcccct gcgcatcgct gacgcgctgc gtgaagccgg cctgcagcga
      481 ctgcaggtcg tggaagtact gcgtgaccga tggctcggcg cgcgccggca cgctcagcag
      541 cagcgcgcat gcgagcagca ggccggcggg aagtctggac agcagggaca tgacaggcct
      601 cgacgaacag ggaacacgga ccgcacagcc gcgcggctgc ggtatcgacc gcggcgccgg
      661 ccgccggtgc gccgccgtta ccggatcgcg acaaaccggc ggccggagtg ctcataccga
      721 tcacatcagc acgcttcgct tttcagcccc tcgcccctcg tgggagaggg gttggggaga
      781 gggggagaaa aaccatgcgt ttatgcgttt acgtgtgatc ggtatcaggc atcggcctgg
      841 gcgccgccga ccagcacctc gcgcgaaccg ttggactgga tcggcccgac gatgccggcg
      901 cgctccatct cctcgaccag ccgtgcggcg cggttgtagc cgatcttcag ccgccgctgc
      961 acgccggaga ccgaggcgcg gcggctctcg acgacgatgc gcacggcctg gtcgtacagc
     1021 ggatcgctgt cgccgtcgct gtcgccgccc ccgccggcca gcggatcgcc cgggaatgcg
     1081 gctccgtcca ccggctcatc gaggatgccg gtcacgtagt cgggctcgcc ggtctgcttc
     1141 aggtactcga cgacggcatt gacctcgtga tcgtcgacga aggccccgtg cacgcgctgc
     1201 gggaagccgg tgccgggggg caggtagagc atgtcgccgt ggccgagcag ggcttcggcg
     1261 cccatctggt cgaggatcgt gcgcgagtcg atcttcgagg cgacggtcag cgcgatgcgc
     1321 gtcgggatgt tggccttgat caggccggtg atcacgtcga ccgacggccg ctgcgtggcg
     1381 aggatcaggt ggatgccggc ggcgcgcgcc ttctgcgcca gccgcgcgat cagctcttcg
     1441 accttcttgc cgacgatcat catcatgtcg gccagctcgt cgatgaccac gaccacgtag
     1501 ggcagcggtt cgagcagcgg ccgttcggga tcgaggctgt cctcgttctt ccacagcggg
     1561 tccgggatcg gctcgccgag ttcgatggcc tcggagacct gcttgttgaa gccggcgatg
     1621 ttgcgcacgc gcagcgccgc catcagccgg tagcggcgct ccatctcggc gacgcaccag
     1681 cgcagcgcgt tggcggcgtc cttcatgtcg gtgaccaccg gcgccagcag gtgcgggatg
     1741 ccctcgtaga tcgacagctc cagcatcttc gggtcgatca ggatcaggcg cagctgctcg
     1801 gcgcccgcct tgtacagcat gctgatcagc atgcagttga ccgccaccga cttgccggag
     1861 ccggtcgtgc cggccaccag cacgtgcggc atcttggcga ggttgccgac caccggccgg
     1921 ccaccgatgt cgtggccgag cgcgaccgtc agcggcgaac ccgctccctg ccagaccggc
     1981 gcggcgatga tctcgcgcag cgacacgatc tgccggcgcc ggttcggaat ctccagtccg
     2041 atcactgact tgccggggat gacctcgacg acgcgcacgc tgaccaccga cagcccgcgc
     2101 gccacgtcct tggcgagatt gctgatctgg ctgaccttga cgccgggggc gaggtcaagc
     2161 tcgaagcgcg tgaccaccgg ccccggttcg acgttgacga cggtcacgtc gacgccgaac
     2221 tcggcgagct tggactcgac ctggcgcgac atgccgtcga gcgcctccgg cgagtagccg
     2281 gcggcctgct cgcgcggcag gtccagaagt tcgagcggcg gcagcgaatc gagcacctcg
     2341 cgcttcggcc gcagcccgaa cagcgccgcc gcagcctcgg acacgccttc ggcacgcgcc
     2401 ggcggctgcg gtcgcggcgc agccgccgga accgccacgg gcgcggcggc cgcgggcgcc
     2461 ggcaggggag
//
LOCUS       DAOQJH010000465         2459 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_31292_length_2459_cov_2.768719, whole genome shotgun sequence.
ACCESSION   DAOQJH010000465 DAOQJH010000000
VERSION     DAOQJH010000465.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2459
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31292_length_2459_cov_2.768719"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..639)
                     /gene="cca"
                     /locus_tag="PLQ18_10385"
     CDS             complement(<1..639)
                     /gene="cca"
                     /locus_tag="PLQ18_10385"
                     /EC_number="2.7.7.72"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011627785.1"
                     /note="catalyzes the addition and repair of the essential
                     3'-terminal CCA sequence in tRNAs without using a nucleic
                     acid template; phosphohydrolase activities include
                     hydrolysis of pyrophosphate, 5'-nucleoside tri- and
                     diphosphates, NADP, and 2'-AMP with the production of Pi,
                     metal-dependent phosphodiesterase activity for 2',3'-cAMP,
                     2',3'-cGMP, and 2',3'-cCMP, and hydrolysis 2',3'-cyclic
                     substrates with the formation of 2'-nucleotides and
                     3'-nucleotides; these phosphohydrolase activities are
                     probably involved in the repair of the tRNA 3'-CCA
                     terminus degraded by intracellular RNases; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional CCA tRNA nucleotidyl
                     transferase/2'3'-cyclic
                     phosphodiesterase/2'nucleotidase/phosphatase"
                     /protein_id="HNE02104.1"
                     /translation="MEIHLVGGAVRDALLGRAVGERDWVVTGATPDELLALGYKRVGK
                     DFPVFLHPETGEEYALARTERKTAPGYHGFAIDAAPHVTLEDDLARRDLTVNAIAQAA
                     DGRLIDPFGGRRDLEARVLRHVSPAFAEDPVRILRLARFAARYAPLGFTVAPETQALM
                     RAMVAAGEVDALQPERVWQELARALGEPVPGRFIEVLRECGALARLLPELDRL"
     gene            794..2410
                     /locus_tag="PLQ18_10390"
     CDS             794..2410
                     /locus_tag="PLQ18_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HNE02105.1"
                     /translation="MNPQNPQNPQDPNLPQSPQASQNPQTPPAATVPPVARFARARHG
                     AGHRRGLALESTGLRRSLLAAALVAAFGAGAVLHGGSELIEQAHAEAPAVTPAPARIV
                     TPDFGALVERYGPAVVNIQVEGTVRTAAQPPQIQIDPNDPLYDFLRRFGLPGLRGRGG
                     PAPDVVRGEGSGFIVRADGVILTNTHVVDGAEHVTVRLTDRREFKARVLGKDDQSDIA
                     VLKIDASDLPTVRIGDPARSRVGDWVLAIGSPFGFENSVSAGIVSAKARTLPDDNYVP
                     FLQTDVAVNPGNSGGPLFNADGEVIGVNSQIYSRTGGYQGISFAIPIDVAMKVEQQIV
                     AHGKVTRGRLGVAIQDVSAPLAAAFGLKTPHGALVSSVEPGSAGEAAGLEAGDVIVAL
                     DGQTIERSADLPPRIADSKPGSHVELTVIRKGEEKTLKATVGEFRAAAREAGDEAAPG
                     QGRLGLALRPLSPQEKRGNDLDSGLLVQQASGPAARAGVAPGDVVLAINGEPVNTVAQ
                     LKERLAHAGKQIALLIQRGEARIFVPVDLG"
BASE COUNT          360 a          931 c          862 g          306 t
ORIGIN      
        1 gagccggtcg agttcgggca ggagccgtgc gagcgcgccg cactcgcgca gcacctcgat
       61 gaagcgcccc ggcaccggtt cgccgagcgc gcgcgccagc tcctgccaga cgcgctcggg
      121 ctgcagcgca tcgacctcgc cggcggcaac catggcgcgc atcagcgcct gcgtttccgg
      181 cgcgaccgtg aagccgagcg gtgcatagcg ggccgcgaag cgcgcaagcc tgaggatgcg
      241 caccgggtcc tcggcgaagg ccggcgacac gtgccgcagc acccgcgctt cgagatcgcg
      301 ccggccgccg aagggatcga tcagccggcc gtcggcggcc tgggcgatgg cgttgacggt
      361 caggtcgcgc cgggcgaggt catcttccag cgtcacgtgc ggggcggcgt cgatcgcgaa
      421 gccgtggtag ccgggcgcgg tcttgcgctc ggtgcgcgcc agcgcgtact cctcgccggt
      481 ttcggggtgc aggaacaccg ggaagtcctt gccgacgcgc ttgtagccga gcgccagcag
      541 ttcatcgggc gtggcgccgg tcaccaccca gtcgcgctcg ccgaccgccc gcccgagcag
      601 cgcatcgcgc accgccccgc cgaccagatg aatttccatc gctgccctcc tcatccttcc
      661 acacaggttc cgcgcaggtt ccacgcacgg ccgttacgcc gcgttaagcc acgggtaagc
      721 cgtggcgaca tcgtcctgcc tatcgtgcgc ccattgaacg cggcagccga agcgcccgcg
      781 aggaggcacg cacatgaacc cgcagaaccc gcagaacccg caggacccga accttccgca
      841 gtccccgcaa gcgtcgcaga acccgcagac gccgccggcc gcgaccgtcc cgcccgtcgc
      901 ccgcttcgcg cgcgcccggc acggtgccgg tcatcgccgc ggactggcgc tcgaatccac
      961 cggcctgcgc cgcagcctgc tcgccgcggc gctggtcgcg gccttcggcg ccggtgccgt
     1021 gctgcacggc ggcagcgagc tgatcgagca ggcgcatgcc gaggctccgg cggtcacgcc
     1081 ggcgccggcg cgcatcgtca cgcccgactt cggcgcactg gtcgagcgct acggcccggc
     1141 cgtcgtcaac atccaggtcg aaggcacggt gcgcacggcc gcgcagccgc cgcagatcca
     1201 gatcgacccg aacgatccgc tgtatgactt cctgcgccgc ttcggcctgc ccggcctgcg
     1261 cgggcgcggc gggcccgcgc cggacgtggt gcgcggcgag ggctcgggct tcatcgtgcg
     1321 cgccgacggc gtgatcctga ccaacaccca cgtcgtcgac ggcgccgagc acgtcaccgt
     1381 gcgcctgacc gaccggcgcg agttcaaggc cagggtgctc ggcaaggatg accagagcga
     1441 catcgccgtg ctgaagatcg acgcgagcga cctgccgacc gtgcgcatcg gcgacccggc
     1501 gcgctcgcgc gtcggcgact gggtgctcgc gatcggcagc ccgttcggct tcgagaacag
     1561 cgtctcggcc ggcatcgtca gcgccaaggc gcgcacgctg cccgatgaca actacgtgcc
     1621 gttcctgcag accgatgtcg cggtcaaccc cggcaactcc ggcggcccgc tgttcaatgc
     1681 cgacggcgag gtcatcggcg tcaactcgca gatctactcg cgcaccggcg gctatcaggg
     1741 catcagcttc gcgatcccga tcgacgtggc gatgaaggtc gagcagcaga tcgtcgcgca
     1801 cggcaaggtc acacgcggcc ggctcggcgt cgcgatccag gatgtctcgg cgccgctcgc
     1861 cgccgccttc ggcctgaaga cgccgcatgg cgcgctggtc agcagcgtcg aacccggcag
     1921 cgccggcgag gccgccggcc tcgaagccgg cgacgtgatc gtcgcgctcg acggccagac
     1981 catcgagcgc tcggccgacc tgccgccgcg catcgccgac agcaagcccg gcagccacgt
     2041 cgagctgacg gtgatccgca agggcgagga aaagacgctg aaggcgacgg tcggcgagtt
     2101 ccgcgccgcc gcccgcgagg ccggcgacga ggccgcgccc ggtcagggcc ggctcgggct
     2161 ggcgctgcgg ccgctgagtc cgcaggagaa gcgcggcaac gacctcgaca gcggcctgct
     2221 ggtgcagcag gcgagcggcc cggccgcgcg cgccggcgtg gcgcccggcg acgtggtgct
     2281 cgcgatcaac ggcgagccgg tcaacaccgt cgcgcagctg aaggagcgcc tcgcgcatgc
     2341 cggcaagcag atcgcgctgc tgatccagcg cggcgaggcc cgcatcttcg tgccggtcga
     2401 tctcggctga ccggtccctc ccgatccatc actgaccccc gcgtgcagcg ccggcaggc
//
LOCUS       DAOQJH010000466         2457 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_31330_length_2457_cov_2.623647, whole genome shotgun sequence.
ACCESSION   DAOQJH010000466 DAOQJH010000000
VERSION     DAOQJH010000466.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2457
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31330_length_2457_cov_2.623647"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1766)
                     /locus_tag="PLQ18_10395"
     CDS             complement(<1..1766)
                     /locus_tag="PLQ18_10395"
                     /inference="COORDINATES: protein motif:HMM:NF016736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HNE02106.1"
                     /translation="MPLPLPNLDDRRFDDLVAELRARLASHVPEWELAPGDPGWALID
                     VLAWLAETILYRANLIPERQRRAFLDLLGLPLRAAAPARGVVCIDSAPNAPQPVPLLP
                     AGSRLRAGAVTCTTETELQPSALELLAVQKARLDAATLGARGVSLDELRALYNRPVTP
                     FEPRALDPATGADFASALDGALWLALLRPRNAPQDAAALRAAFAGRTLSLGLAPDEEA
                     PDGLVARALPARSLKLELVHARSGAEPGWLALEILDDRSLGARQPGVLRFRLPANALL
                     APPLYDDPKDAGLGVRPPALPAGRSGDELVHWLRLSCPDAPGLKLAWLGFNAVDVVAL
                     DQRRDVLLAVGDGRPDQVCALPDGNVDAASLLLEVETPQTTAAGWERWSPVERFAAAG
                     REAPVYALDAASGRIRFGDGIRGRVPAPGRRIRAVEYRHGGGAAGNLAPGQLRSVVEA
                     PMLRVRHERALRGGADAETIAEAERRLPDWLNHRERAVTAADFRMLALETPGSGIARA
                     EVVRGLIPGDTLAALQRNVPGAVAVFVLAARAPDDPAPPRPGAAQLREVWAWLRERCP
                     IGTELYVLSPRFVPLAVSVAVGV"
     gene            complement(1782..2213)
                     /locus_tag="PLQ18_10400"
     CDS             complement(1782..2213)
                     /locus_tag="PLQ18_10400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015438131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HNE02107.1"
                     /translation="MDRQHDGPVRAARGWPLGGLPDPADAGAGATAGQLAWTSGERAI
                     RESLINILLTRPGERLQRPDFGAGVRDWIHRPNDATSRGLLAAVIERALRRDEPRIAL
                     EAVEVVAPPDDPTQIGITIRYRILASGQRDGLALTLAPERP"
     gene            complement(2213..>2457)
                     /locus_tag="PLQ18_10405"
     CDS             complement(2213..>2457)
                     /locus_tag="PLQ18_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007686320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02108.1"
                     /translation="DTGSLIAGLRGKDRAKDALDGNASQGAGFGFGMLCSFSLPILTL
                     CALILLMVIVALLNFVFRWLPFVYLCIPWPKREGDG"
BASE COUNT          322 a          874 c          978 g          283 t
ORIGIN      
        1 acgccgaccg cgaccgagac cgccagcggc acgaagcgcg gcgacagcac gtagagttcg
       61 gtgccgatcg ggcagcgttc gcgcagccag gcccagacct cgcgcagctg cgcggcgccc
      121 ggccgcggcg gggccgggtc gtcgggggcg cgcgccgcca gcacgaacac cgcgactgca
      181 ccggggacgt tgcgctgcag ggcggccagc gtgtcgccgg ggatcagccc gcgcacgacc
      241 tcggcgcggg cgatgccgct gcccggggtt tcgagcgcga gcatccggaa gtcggcggcg
      301 gtcaccgcgc gttcgcggtg gttcagccag tccggcagcc ggcgttcggc ctcggcgatg
      361 gtctcggcgt cggcaccgcc gcgcagcgcg cgttcgtggc gcacgcgcag catcggcgcc
      421 tcgacgacgc tgcgcagctg ccccggcgcc agattgccgg ccgcgccgcc gccgtggcgg
      481 tattcgacgg cgcggatgcg ccggcccggt gccggcacgc ggccgcggat gccgtcgccg
      541 aagcggatgc ggccgctcgc ggcatcgagt gcgtagacgg gcgcctcgcg cccggcagcg
      601 gcgaagcgct cgaccggcga ccagcgctcc cagccggccg cggtggtctg cggcgtctcg
      661 acttcgagga gcagcgatgc ggcatcgacg ttgccatcgg gcagcgcgca gacctgatcc
      721 ggccggccgt cgccgacggc gagcagcacg tcgcggcgct ggtcgagcgc gacgacatcg
      781 acggcgttga agccgagcca cgcgagcttc aggcccggcg cgtcggggca ggacaggcgc
      841 agccagtgca cgagttcgtc gccgctgcgc ccggccggca gcgccggcgg acgcacgccg
      901 aggccggcat ccttcggatc gtcgtagagc ggcggtgcga gcagcgcgtt ggccggcagc
      961 cggaagcgca gcacgccggg ctggcgggcg ccgagcgagc ggtcatcgag gatttccagc
     1021 gccagccagc cgggttcggc gccgctgcgt gcatgcacga gttcgagctt caggctgcgg
     1081 gccggcagcg cgcgcgcgac cagcccgtcg ggtgcttcct cgtccggcgc gaggccgagc
     1141 gacagcgtgc ggccggcgaa ggccgcgcgc agcgccgcgg cgtcctgcgg cgcgttgcgc
     1201 gggcgcagca gcgccagcca cagcgcgccg tcgagggcgg acgcgaagtc ggcaccggtg
     1261 gccggatcga gcgcgcgcgg ctcgaacggc gtcaccggcc ggttgtagag cgcgcgcagt
     1321 tcatcgagcg acacgccgcg cgcaccgagc gtcgcggcat cgagccgtgc cttctgcacc
     1381 gccagcagtt cgagcgcgct cggctgcagc tcggtttcgg tcgtgcaggt gacggcgccg
     1441 gcgcgcagcc ggctgccggc cggcagcagc ggcaccggct gcggcgcgtt cggcgcgctg
     1501 tcgatgcaga cgacgccgcg cgccggggcc gcggcgcgca gcggcaggcc gagcaggtcg
     1561 aggaaggcac ggcgctggcg ttccgggatc aggttggcgc ggtacaggat cgtctcggcg
     1621 agccaggcca gcacgtcgat cagcgcccag cccggatcgc cgggcgcgag ttcccattcg
     1681 ggcacgtgac tcgcgaggcg cgcgcgcagc tcggcgacca gatcgtcgaa gcggcggtcg
     1741 tcgaggttcg gcagcggcag cggcatggcg gctccggacg gtcaggggcg ttcgggggcc
     1801 agcgtcagcg cgaggccgtc gcgctggccg ctggcgagga tgcggtagcg gatcgtgatg
     1861 ccgatctgcg tcgggtcatc gggcggggcg acgacctcga cggcttcgag cgcgatgcgc
     1921 ggctcgtcgc gccgcagggc ccgctcgatg acggcggcga gcaggccgcg gctggtggcg
     1981 tcgttcggac gatggatcca gtcgcgcacg ccggcgccga agtccggacg ctgcaggcgc
     2041 tcgccgggcc gggtcagcag gatgttgatc aggctctcgc ggatcgcgcg ctcgccgctc
     2101 gtccacgcga gctgcccggc cgtcgcaccg gcgccggcat cggccggatc gggcaggccg
     2161 ccgagcggcc agccgcgggc tgcgcgcacc gggccatcgt gttgccggtc catcagccgt
     2221 ccccctcgcg cttcggccac gggatgcaca gataaacgaa gggcagccag cggaagacga
     2281 aattcagcag cgcgacaatg accatcagca ggatcagcgc gcacagcgtc aggatcggca
     2341 ggctgaacga gcagagcatg ccgaagccga agccggcgcc ctgactggcg ttgccgtcga
     2401 gggcgtcctt cgcgcggtcc ttgccgcgca ggccggcgat caggctgccg gtgtcgg
//
LOCUS       DAOQJH010000467         2449 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_31479_length_2449_cov_3.954470, whole genome shotgun sequence.
ACCESSION   DAOQJH010000467 DAOQJH010000000
VERSION     DAOQJH010000467.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2449)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2449)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2449
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31479_length_2449_cov_3.954470"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /gene="narH"
                     /locus_tag="PLQ18_10410"
     CDS             <1..207
                     /gene="narH"
                     /locus_tag="PLQ18_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015258540.1"
                     /note="with NarGJI catalyzes the reduction of nitrate; the
                     beta subunit is an iron sulfur cluster containing electron
                     transfer subunit; one of 3 nitrate reductases in E. coli
                     and in E. coli is expressed when nitrate levels are high;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HNE02109.1"
                     /translation="LAIANYEDRFVIPTSHREYASATYDAFSERGGCGFSFGNGCADG
                     GSKLNLFGGKKQTTRRANPIRFVK"
     gene            236..958
                     /gene="narJ"
                     /locus_tag="PLQ18_10415"
     CDS             236..958
                     /gene="narJ"
                     /locus_tag="PLQ18_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015258539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HNE02110.1"
                     /translation="MLTLKIVSALLCYPQPDLMDALPELAALVREDDELPVVERGRLV
                     DFIDELADSDLWTLQEHYVARFDRGRKLSLHLFEHIHGESRDRGQAMVDLLDVYRGAG
                     FELKARELPDYLPLFLEFLATRPHEEARRLLDDASHVIALLGARLAERHDPYAVLFDA
                     LLALIELPADLGEIRAQAASEGPDRTITDMDKIWEEEQVTFLASQAPDGGCGVAAAGT
                     AQPLAFVPPRQMRAPAPSQPSR"
     gene            992..1672
                     /gene="narI"
                     /locus_tag="PLQ18_10420"
     CDS             992..1672
                     /gene="narI"
                     /locus_tag="PLQ18_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006747608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HNE02111.1"
                     /translation="MSYLNDLLFGVYPYIAGTVFLIGSLLRFDRDQYTWKAHSSQMLS
                     NAGFRRASNLFHIGIIGIFFGHLAGLLTPVAVWHALGVSASFKQWVAIVLGGLFGTMC
                     FIGLTALVHRRLNDARVRANSSRMDIVILLLLYGQLILGLLTIPVSLFHLDGANMLTL
                     MAWAQHIVTFDGPGAAAIAANVGGLFKLHLLLGMTIFLLFPFSRLVHVWSVPLGYVKR
                     PYQIVRQR"
     gene            1742..2419
                     /locus_tag="PLQ18_10425"
     CDS             1742..2419
                     /locus_tag="PLQ18_10425"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010398573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HNE02112.1"
                     /translation="MPDISAFVSGFLKFQAEHFGEDGLFETLRHGQHPSTLLIGCCDA
                     RVDPALLTGCKPGDIFIVRNVANLVPPCTPDAPPGVSSAIEFAICGLGVERIIVLGHA
                     RCGGIRYLMDPQPLTDETDFLGRWMQIAEPARTRVLHELAHKSPEERLRALELASILL
                     SLDNLMSYPWLRRRVEAGGLTLHGWYFDIAEGALLAYSARRQQFLPVVCPLDRSTHRK
                     YDAGPTP"
BASE COUNT          355 a          902 c          789 g          403 t
ORIGIN      
        1 ctggcgatcg ccaactacga ggaccgcttc gtgatcccga cttcgcaccg cgagtacgcc
       61 tcggcgacct acgacgcctt cagcgagcgc ggcggctgcg gtttcagctt cggcaacggc
      121 tgcgccgatg gcggcagcaa gctgaatctc ttcggcggca aaaagcagac gacccgccgc
      181 gccaacccga tccgcttcgt caagtgaagc accggacacc cgaggaatac gcatcatgct
      241 gacgctgaag atcgtgtccg cgctgctctg ctacccgcag ccggatctca tggacgccct
      301 gcccgaactg gccgcactgg tgcgcgagga cgatgaactg ccggtcgtcg agcgcggccg
      361 gctggtggac ttcatcgacg aactggccga ttccgacctc tggacgctgc aggagcatta
      421 cgtcgcgcgc ttcgaccgcg gccgcaagct gagcctgcac ctgttcgagc acatccacgg
      481 cgaatcgcgc gaccgcggtc aggcgatggt cgatctgctc gatgtctacc gcggcgccgg
      541 cttcgagctg aaggcccggg agctgccgga ctacctgccg ctgttcctcg aattcctggc
      601 cacgcgcccg cacgaggagg cgcgccgcct gctcgatgac gcgagccacg tcatcgcgct
      661 gctcggtgcg cgcctggccg agcgccacga cccgtacgcg gtgctgttcg acgcgctgct
      721 ggcgctgatc gagctgcccg cagacctcgg cgagatccgc gcacaggccg ccagcgaagg
      781 cccggaccgc acgatcaccg acatggacaa aatctgggag gaggaacagg tgaccttcct
      841 cgccagccag gcgccggacg gcggctgcgg cgtcgcggcg gccggcacgg cgcagccgct
      901 ggccttcgtg ccgccgcggc agatgcgcgc gccggcgcct tcgcagccgt cccgctgagc
      961 cgccggctcc tgaatcctga ggaattccgt catgtcctac ctgaacgacc tgctgttcgg
     1021 cgtctacccg tacatcgccg gcaccgtgtt cctgatcggc agcctgctgc gcttcgaccg
     1081 cgaccagtac acgtggaagg cgcattcgag ccagatgctg tcgaacgccg gcttccggcg
     1141 cgccagcaac ctgttccaca tcggcatcat cggcatcttc ttcggccatc tggccggcct
     1201 gctgacgccg gtcgcggtct ggcacgcgct cggcgtgtcg gcgagcttca agcagtgggt
     1261 ggcgatcgtg ctcggcggcc tgttcggcac gatgtgcttc atcggcctga cggcgctggt
     1321 gcaccggcgc ctgaacgatg cgcgcgtgcg tgcgaattcg agccgcatgg acatcgtgat
     1381 cctgctgctg ctgtacgggc agctgatcct cggcctgctg accatcccgg tgtcgctgtt
     1441 ccacctcgac ggtgccaaca tgctgacgct gatggcctgg gcgcagcaca tcgtcacctt
     1501 cgacggcccg ggcgccgcgg cgatcgccgc caacgtcggc gggctgttca agctgcacct
     1561 gctgctcggc atgacgatct tcctgctgtt cccgttcagc cgcctcgtac acgtctggag
     1621 cgtgccgctc ggctacgtga agcgcccgta ccagatcgtg cgccagcgct gaacggccgc
     1681 ctgtccgggc ggggaagacg cgcaagccgc cttccccgcc gtcttgcccg gtagcgccgc
     1741 catgcctgac atctccgcct tcgtttccgg cttcctgaag ttccaggccg agcacttcgg
     1801 tgaggacggc ctgttcgaga cgctgcgcca cggccagcac ccgtcgacgc tgctgatcgg
     1861 ctgctgcgac gcccgcgtcg atccggcgct gctgaccggc tgcaagcccg gcgacatctt
     1921 catcgtgcgc aacgtcgcca acctcgtgcc gccgtgtacg ccggacgccc cgcccggcgt
     1981 cagttcggcg atcgagttcg cgatctgcgg cctcggggtc gagcgcatca tcgtgctcgg
     2041 ccatgcgcgc tgcggcggca tccgctacct gatggacccg cagccgctga ccgacgagac
     2101 cgacttcctc ggccgctgga tgcagatcgc cgaaccggca cgcacgcgcg tgctgcacga
     2161 actcgcgcac aagtcgcccg aggagcgcct gcgcgcgctg gagctggcga gcatcctgct
     2221 gtcgctcgac aacctgatga gctacccgtg gctgcgccgg cgcgtcgagg ccggcgggct
     2281 gacgctgcac ggctggtatt tcgacatcgc cgaaggggcg ctgctcgcct actctgcgcg
     2341 ccgccagcag ttcctgcccg tggtctgccc gctcgatcgc agcacgcacc gcaagtacga
     2401 cgccggcccg acgccctgac ccggccgccg cgaccgcccg cctccgccc
//
LOCUS       DAOQJH010000468         2443 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_31617_length_2443_cov_4.299414, whole genome shotgun sequence.
ACCESSION   DAOQJH010000468 DAOQJH010000000
VERSION     DAOQJH010000468.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2443)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2443)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2443
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31617_length_2443_cov_4.299414"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..399)
                     /locus_tag="PLQ18_10430"
     CDS             complement(<1..399)
                     /locus_tag="PLQ18_10430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02113.1"
                     /translation="MSSQDSQRRTAAIFWSLLIGGLALRLIFFAGPIGSDDSNYYMYS
                     EQLLNGRLEGSAHHHASRMLFMLLLGIPTVLSHSLYTGCMLSIAVSIAGGLLVYAFMR
                     RHASMNAALVAFGMVMLSGTSILYASVFVPD"
     gene            complement(901..1431)
                     /locus_tag="PLQ18_10435"
     CDS             complement(901..1431)
                     /locus_tag="PLQ18_10435"
                     /inference="COORDINATES: protein motif:HMM:NF023363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3455 domain-containing protein"
                     /protein_id="HNE02114.1"
                     /translation="MNRSACIVRSLALSAALAAAGCASVQPPETPDYLKPPADQSAYL
                     MTRGSGDYLYECRATPGAPKGHAWQFKATEATLSDADGKPVGRIGAGPLWTAADGSRF
                     TGVPQARKPVEQDKAVPWLLLGTKDNSGDGVFGRTKSVQRVDTEGGLFPNAPCTRRQA
                     GQIARVPYKATYYYYR"
     gene            1693..>2443
                     /locus_tag="PLQ18_10440"
     CDS             1693..>2443
                     /locus_tag="PLQ18_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011390216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HNE02115.1"
                     /translation="MTVSLSRLLSSDDPLPVSVLNPGAHSPLLLLCDHASHAVPAALA
                     GLGLPAAELTRHIGWDPGAALVSAHLAGRFDAAAVLSGFSRLVIDCNRAPGDPTSIPE
                     ISDGTVVPGNRGLDEAAVLARLDACFWPYHQVITARIAQMRSDGRVPVVVAVHSFTPC
                     LNCAGGARPWQIGVLWNHDPRLAVPLIETLRAQGVCVGDNEPYSGRDSSYTVETHAAA
                     AGLPHVLIELRQDLLGDPAGCAHWADVLGDAL"
BASE COUNT          380 a          798 c          840 g          425 t
ORIGIN      
        1 gtccggtacg aacacgctcg catacaggat gctggtgccc gagagcatga ccatgccgaa
       61 ggccacgagc gccgcgttca ttgaggcgtg gcggcgcatg aatgcgtaga cgagcaggcc
      121 cccggcgatc gacaccgcaa tcgacagcat gcagccggta tacaggctgt ggctcagcac
      181 ggtcggaatg ccgagcagca gcatgaacag catccgggaa gcatgatggt gggcggaccc
      241 ttcgagtcgg ccgttcagca gctgttcgga atacatataa tagttggaat cgtcagaacc
      301 gatcgggccg gcaaagaaga tcagccgcag ggccagcccg ccgatcagca aactccagaa
      361 gattgccgcg gtccgtcgct ggctgtcctg actcgacatc gtcgcctccg aaactgctgt
      421 gcttcggcga gtatgtcatc gggcggcctc gaacagtatg gccttgccgc ccgctgcggc
      481 ttccgtggtc cgcggcagga ctggtgcagg acaaacacat cttcttgcaa tgcgaaacag
      541 ccgctgagag gtaggcattt ctggtgaagt ctggtcggaa tccagacctc gccggaccga
      601 agttttcaga aacttacagg gaagctgcta tcacgcttct gcttgacttt tcgcatgacg
      661 agaagatgcg tttgccttgg gaccggtgcg attcccgcaa aaggtttccg cacaatgagg
      721 aaatcccgcc gggaattcgg aaaatttttc ccggcggaga cgattttttt caaacatttt
      781 cctccctgcc cccgcgggtc ggtgccggca atgcatgaaa gggattttcc gctgacgaca
      841 agatgcgccg gcgcggcggt ccgcggcaca ccgccgccac tgcgccggcc acgaggaggt
      901 tcagcggtag tagtaatacg tcgccttgta cggcacgcgc gcgatctggc cggcctgcct
      961 gcgcgtacac ggcgcgttcg ggaacaggcc gccttcggtg tcgacgcgct gcacgctctt
     1021 cgtgcggccg aacacgccat ccccgctgtt gtccttcgtg ccgagcagca gccacggcac
     1081 ggccttgtcc tgttcgaccg gcttgcgggc ctgcggcacg ccggtgaagc ggctgccgtc
     1141 ggcggccgtc cacagcgggc cggcgccgat cctgccgacc ggcttgccgt cggcgtcgct
     1201 cagcgtcgcc tcggtggcct tgaactgcca ggcgtggccc ttcggggcgc ccggggtcgc
     1261 gcggcattcg tacaggtaat cgccgctgcc gcgggtcatc aggtaggcac tctggtcggc
     1321 cggcggcttc aggtagtccg gggtttcggg cggctggacg ctcgcgcagc cggcggcggc
     1381 gagggcggcg gacagcgcga ggctgcgcac gatgcaggcg gatcggttca tgtggttatc
     1441 tccgggtggt gatgcggcgc gacgacggcg cgcggcgaac cggaatgcgc aggccgtcgt
     1501 cgccggcaac tgtagaagcg cgcccgcacg ccggtgatta ccggccgttg aatggcgggc
     1561 aatgacgtgc aatgacgttg aatgacaccg ggcggcgggc cagggaaccg ggcgctgcgg
     1621 gcagggttca ctacgctggc tgtgaacctt gcgctgtcgc cctgtccttc agggccttcg
     1681 ggaatgtccg cgatgaccgt atccctgtcc cgcctgctga gctccgatga tccgctgccg
     1741 gtcagcgtgc tgaatcccgg cgcgcactcg ccgctgctgc tgctctgtga tcacgccagc
     1801 cacgccgtgc cggctgcgct tgccggactc ggcctgccgg cggcggaact gacgcgccac
     1861 atcggctggg accccggtgc ggcgctggtc agcgcgcatc tggccgggcg cttcgatgcc
     1921 gcggcggtgc tgtcgggctt ttcgcggctg gtcatcgact gcaaccgcgc gccgggcgac
     1981 ccgacctcga tcccggaaat cagtgacggc accgtcgtgc ccggcaaccg ggggctggac
     2041 gaggcggcgg tgctcgcgcg gctcgatgcc tgcttctggc cgtatcacca ggtcatcacc
     2101 gcgcgcatcg cgcagatgcg cagcgatggc cgcgtgccgg tcgtcgtcgc cgtgcacagc
     2161 ttcacgccct gcctgaactg cgccggcggc gcgcggcctt ggcagatcgg cgtgctgtgg
     2221 aaccacgacc cgcgtctggc cgtgccgctg atcgagaccc tgcgcgcgca gggcgtctgc
     2281 gtcggtgaca acgaaccgta ttcggggcgc gattcgagct acacggtcga gacccacgcc
     2341 gccgccgccg gcctgccgca cgtgctgatc gagctgcgtc aggacctgct cggcgatccg
     2401 gccggctgtg cgcactgggc cgacgtgctc ggcgatgcgc tgg
//
LOCUS       DAOQJH010000469         2438 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_31734_length_2438_cov_4.258498, whole genome shotgun sequence.
ACCESSION   DAOQJH010000469 DAOQJH010000000
VERSION     DAOQJH010000469.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2438
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31734_length_2438_cov_4.258498"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..797
                     /locus_tag="PLQ18_10445"
     CDS             <1..797
                     /locus_tag="PLQ18_10445"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017915645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HNE02116.1"
                     /translation="TDFGDDKDRVMRKRDGTFTYFLPDVAYHRTKWERGHVRAITELG
                     ADHHGSLARVRAGLRALDIGIPTGWPEYVLHQMVTVMRGGEEVKISKRAGGYVTLRDL
                     IDEVGRDATRFFFLMRKADSHLVFDVDLARQQSLDNPVYYLQYAHARVCSVLRQAGEK
                     GLDYDEAAGFAALARLVEPHEETLLTLLDRYPEVIELAAGSDEPQLLPAYLRELAQAF
                     HAYYNAHAFLVDDAALRNARLALILAVRRVLASGLDLLGVSAPETM"
     gene            808..1578
                     /locus_tag="PLQ18_10450"
     CDS             808..1578
                     /locus_tag="PLQ18_10450"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HNE02117.1"
                     /translation="MSVRRDYKPRTAEKKPANRGVRVLGWAVVTLALFGAGAGGWGWW
                     QKHRAAKAEEAAAAAAAAGQAGPAGGKPSLATLKAQDARAVGSSSTAARPGALPELPP
                     SRFSFYHDLQKRQVQIQQDEIGRSGKGPVPEGALNAQTIVATPPAQAPAPAPKPAPAA
                     SPPPAAAQQTASATPAASGRGYLVQAGVFSTSAQAERVRASLALLGVAARIEAGTTAD
                     GAAVSRVRLGPYKDAAQAKAVTDKLGEAGIKAIAIKLD"
     gene            complement(1594..2130)
                     /locus_tag="PLQ18_10455"
     CDS             complement(1594..2130)
                     /locus_tag="PLQ18_10455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010342824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HNE02118.1"
                     /translation="MNPRLLPVALAAALALGPALADEAPLPVATQLDLDRYAGRWYEI
                     ARLPMPYEDDCTGDVRAEYARRPDGGIEVINRCRTAAGESIARGSARVLGPGRLQVRF
                     APDWLAWLPWVWADYQVIELDPQYRWALVGQPSRRYLWVLARTPQLDAAVTEGLLARA
                     RELGFDLTELIRTPQDGG"
BASE COUNT          354 a          879 c          883 g          322 t
ORIGIN      
        1 cgaccgactt cggcgacgac aaggatcgcg tgatgcgcaa gcgcgacggc acgttcacgt
       61 acttcctgcc cgatgtcgcc taccaccgca ccaagtggga gcgcggccac gtgcgcgcga
      121 tcaccgagct cggcgccgac caccacggct cgctcgcgcg cgtgcgcgcc ggcctgcggg
      181 cgctcgacat cggcatcccg accggctggc ccgagtacgt gctgcaccag atggtcaccg
      241 tgatgcgcgg cggcgaggag gtgaagatct ccaagcgcgc cggcggttac gtgacgctgc
      301 gcgatctgat cgacgaggtc ggacgcgatg cgacgcggtt tttcttcctg atgcgcaagg
      361 ccgattcgca tctggtgttc gatgtcgatc tggcccgcca gcagtcgctc gacaacccgg
      421 tctactacct gcagtacgcg catgcgcgcg tctgcagcgt gctgcgtcag gcgggcgaga
      481 aggggctcga ctacgacgag gcggccgggt tcgcggcgct ggcgcggctg gtggagccgc
      541 acgaggaaac cctgctgacg ctgctcgacc gctatccgga agtcatcgaa cttgcagccg
      601 gcagcgacga gccgcagctg ctgccggcct atctgcgcga actcgcgcag gccttccatg
      661 cctactacaa cgcgcacgcc ttcctggtcg atgacgcggc gctgcgcaac gcccgcctcg
      721 ccctgatcct cgcggtgcgc cgcgtgctcg ccagcggcct cgacctgctc ggcgtttcgg
      781 cgccggagac catgtgagcc atagccgatg agcgtgcgcc gcgactacaa gcccaggacg
      841 gctgaaaaga agcctgccaa ccgcggcgtg cgcgtgctcg gctgggccgt cgtcacgctg
      901 gcgctgttcg gtgccggagc cggcggctgg ggctggtggc agaagcacag ggcggcaaag
      961 gccgaggagg cggccgccgc agcggcagcg gccggtcagg ccggcccggc cggcggcaag
     1021 ccgtcgctgg cgacgctgaa ggcgcaggat gcgcgcgcgg tcggcagctc ctcgaccgcc
     1081 gccaggcccg gcgcgctgcc cgagctgccg ccgtcgcgct tttcgttcta tcacgacctg
     1141 cagaagcgtc aggtgcagat ccagcaggac gagatcggcc gcagcggcaa gggtccggtg
     1201 cccgaaggcg cgctgaacgc gcagaccatc gtcgccaccc cgccggcgca agctccggcg
     1261 ccggctccga aaccggctcc ggccgccagc ccgccgccgg ccgccgcgca gcagacggcg
     1321 agcgccacgc ccgccgccag cggtcgcggc tatctggtgc aggccggcgt gttctcgacc
     1381 agcgcgcagg ccgagcgcgt gcgcgcgagc ctcgcgctgc tcggcgttgc agcgcgcatc
     1441 gaggccggca cgaccgccga tggcgccgcc gtcagccgcg tgcgcctcgg gccgtacaag
     1501 gacgccgccc aggccaaggc cgtcaccgac aagctcggcg aggccggcat caaggcgatc
     1561 gcgatcaagc tcgactgacg ggcagggcgg atttcagccg ccgtcctgcg gcgtgcggat
     1621 cagctccgtc agatcgaagc cgagttcgcg cgcccgcgcg agcaggcctt ccgtgaccgc
     1681 ggcatcgagc tgcggcgtgc gcgccagcac ccacaggtag cggcgcgaag gctggccgac
     1741 cagcgcccag cggtactgcg ggtcgagttc gatgacctgg taatcggccc agacccaggg
     1801 cagccacgcg agccagtccg gtgcgaagcg cacctgcagc cggccggggc cgagcacgcg
     1861 ggcgctgccg cgggcgatcg attcgccggc ggccgtgcgg cagcggttga tgacttcgat
     1921 gccgccatcc gggcggcggg catattcggc gcgcacgtcg ccggtgcagt cgtcctcgta
     1981 cggcatcggc agccgggcaa tctcgtacca gcgcccggcg tagcgatcga ggtcgagctg
     2041 cgtggccacc ggcagcgggg cctcgtcggc aagggcaggg cccagggcca gggcggcggc
     2101 gagcgcgaca ggcagcaggc ggggattcat cggggcggct ccggggtttt gcgggtgccg
     2161 gcagccgacc gccgccggcg ccgccggttc ccgctgcctc aggttgatca cgcaagcctg
     2221 cgtctttcag tccttcgccc ctcccgggag acagcatcgg tatctacaca agtcagcggt
     2281 tccgcgggcc ggatggatcg accgatcaac gcccgggcgt cgatctcccc tctccccttg
     2341 cgggagaggg gccgggggag agggggagac gccggcactg cgcccggacc ttgcccccct
     2401 ctccccaacc cctcccccgc gaggggggag gggcttgt
//
LOCUS       DAOQJH010000470         2438 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_31736_length_2438_cov_3.892572, whole genome shotgun sequence.
ACCESSION   DAOQJH010000470 DAOQJH010000000
VERSION     DAOQJH010000470.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2438
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31736_length_2438_cov_3.892572"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2438
                     /locus_tag="PLQ18_10460"
     CDS             <1..>2438
                     /locus_tag="PLQ18_10460"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNE02119.1"
                     /translation="ADILARHFTEVPGGYVIAPAIRARMVFARHDVLSAPPFLNLDLV
                     TCRNLFIYLRPAAQERLLAVFHAALGSEGLLLLGRRESIRTESPLFTPLDGSARLFVR
                     RPARAHPPTGARCTPTLPTLPAAARNRLGLYGAVITALAPNAVVIDETGNCLHFFGEV
                     RSFLGFVRNEPDLRLANLLPEPWRPAYQVALSRARRATVKDGIDSVDLPQITGYGSAT
                     GRWQLSIRPLHGEELGHGWLVSFEAQSVLAKDPAHNSGIEGNIGFESELDDLNRRYQE
                     ALAAAATVNDELQATNEEMQAANEELEATSEEMQAANEELESTNTELEIRLNELAVLN
                     AELNEILGSAGQMQIVLDADLRVCRFSPSATRLYALDQNDIGRPLALVQPIEPLPGLD
                     ARLRRVLSTGLPLMEEIDAGDRALMLQITPVGKSAATRRLILGATDTTALRSARRAQQ
                     IGEERLRESENRFQRIVEAIPVGIAQHDPNGDVRFLNPYFTQLFGYSIEDIPTVEDWY
                     RLAWPDADARARARQQWQEDLRAQRLGLDTPAREQQIRCRDGNLRSVEIALARGGSGP
                     YDGIYAVFSDVTEQREVMQRLEQAMLAAQSAARAKSEFLANMSHEIRTPMNAIIGLSE
                     LALHGELPPRQHQYVQRIHQAGVSLLGVVNDILDFSKIEAGKLSIEQAPFSLGRLLRD
                     VLDLVAGNAHTRGLYLYASVDPRVPDALTGDALRLQQVLLNLLGNAVKFTPQGGIVLS
                     IRPGGSPRATTLQREFVFEVTDTGIGIEAEERQRLFQPFEQSDPSTTRRYGGTGLGLT
                     ICRRLASLMEGRLD"
BASE COUNT          399 a          896 c          804 g          339 t
ORIGIN      
        1 cgccgatatc ctcgcccggc acttcaccga ggtgcccggc ggctacgtga tcgctccagc
       61 gatccgggcg cgcatggtgt tcgcacgcca tgacgtgctc tcggccccgc cgttcctgaa
      121 cctcgatctc gtcacctgcc gcaacctgtt catctacctg cgccccgccg cccaggaacg
      181 gctgctcgcc gtcttccatg ccgccctcgg cagcgaaggc ctgctgctgc tcgggcggcg
      241 cgaatcgatc cgcaccgaaa gcccgctgtt cacaccgctc gatggcagtg cccggctgtt
      301 cgtgcgccgg cccgcccggg cccacccccc gaccggagca cgctgtacgc cgacgctgcc
      361 gacgctgccg gcagcggcca gaaaccggct cgggctgtac ggggccgtga tcacggcgct
      421 ggccccgaat gcggtcgtga tcgacgagac cggcaactgc ctgcacttct tcggcgaggt
      481 caggagcttt ctcggcttcg tgcgcaacga acccgacctg cgcctcgcga acctgctgcc
      541 ggaaccgtgg cgaccggcct atcaggtggc gctgagccgg gcccggcgcg ccaccgtcaa
      601 ggacggcatc gacagcgtcg atctgccgca gatcaccggc tacggctcgg ccaccggccg
      661 ctggcagctc agcatccggc cgctgcacgg ggaggagctc ggccatggct ggctggtgag
      721 cttcgaggcc cagtcggtgc tggcgaagga cccggcgcac aactccggca tcgagggcaa
      781 catcggcttc gaaagcgagc tcgatgacct gaaccgccgc tatcaggaag cgctcgccgc
      841 cgccgccacc gtcaacgacg aactgcaggc caccaacgag gaaatgcagg ccgccaacga
      901 ggagctcgaa gccacctccg aggaaatgca ggccgccaac gaggagctgg aaagcaccaa
      961 caccgagctg gaaatccggc tcaacgaact ggcggtgctc aacgccgagc tgaacgagat
     1021 tctcggctcg gccggccaga tgcagatcgt gctcgatgcc gacctgcgcg tctgccgctt
     1081 cagcccgtcc gcgacgcgcc tgtacgcgct cgaccagaac gacatcggcc ggccgctggc
     1141 gctggtgcag ccgatcgagc ccctgcccgg actcgacgcg cggctgcgtc gggtgctgag
     1201 cacggggctg ccgctgatgg aggagatcga cgccggcgac cgcgcgctga tgctgcagat
     1261 caccccggtc ggcaagagcg cggccacgcg ccggctgatc ctcggtgcca ccgacacgac
     1321 ggcgctgcgc agtgcccggc gcgcccagca gatcggcgag gagcgcctgc gcgagagcga
     1381 gaaccgcttc cagcgcatcg tcgaggcgat tccggtcggc atcgcgcagc acgatcccaa
     1441 tggcgacgtg cgcttcctga acccgtattt cacgcagctg ttcggctact cgatcgagga
     1501 catccccacc gtcgaggact ggtaccggct cgcatggccg gacgccgacg cgcgggccag
     1561 ggcccgccag cagtggcagg aggacctgcg cgcgcaacgc ctcggcctcg acactccggc
     1621 ccgcgagcag cagatccgct gccgcgacgg caacctgcgc agcgtcgaga ttgcgctcgc
     1681 acggggcggc agcgggccgt acgacggcat ctatgcggtg ttcagcgatg tcaccgagca
     1741 gcgcgaggtc atgcagcgcc tcgaacaggc gatgctggcc gcgcagtcgg ccgcgcgggc
     1801 caagtccgaa ttcctcgcca acatgagcca cgagatccgc acgccgatga acgccatcat
     1861 cgggctgtcg gaactcgcgc tgcacggcga gctgccgccg cgccagcacc agtacgtgca
     1921 gcgcatccat caggctggcg tgtcgctgct cggcgtcgtc aacgacatcc tcgacttctc
     1981 gaagatcgag gccggcaagc tgtcgatcga gcaggcaccg ttctcgctcg gccggctgct
     2041 gcgcgacgtg ctcgatctgg tcgccggcaa cgcccacacg cgcgggctgt acctgtacgc
     2101 cagcgtcgat ccgcgcgtac ccgatgccct gaccggcgat gcgctgcgcc tgcagcaggt
     2161 gctgctgaac ctgctcggca atgccgtcaa gttcacgccg cagggcggca tcgtgctgtc
     2221 gatccggccg ggcggcagcc cgcgggcgac cacgctgcag cgcgagttcg tgttcgaggt
     2281 caccgacacc ggcatcggca tcgaagcgga ggagcgccag cgcctgttcc agcccttcga
     2341 acagtccgac ccctcgacga cccggcgcta cggcggcacc ggcctcgggc tcacgatctg
     2401 ccgccggctg gcgagcctga tggaaggccg gctggacc
//
LOCUS       DAOQJH010000471         2434 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_31830_length_2434_cov_2.707020, whole genome shotgun sequence.
ACCESSION   DAOQJH010000471 DAOQJH010000000
VERSION     DAOQJH010000471.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2434)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2434)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2434
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31830_length_2434_cov_2.707020"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1478
                     /gene="infB"
                     /locus_tag="PLQ18_10465"
     CDS             <1..1478
                     /gene="infB"
                     /locus_tag="PLQ18_10465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006082716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HNE02120.1"
                     /translation="HVDHGKTSLLDYIRRTKVAAGEAGGITQHIGAYHVETGKGTITF
                     LDTPGHAAFTAMRARGAKATDIVVLVVAADDGVMPQTVEAIQHARAAGVPIVVAVNKM
                     DKNGIDLERVKSELSQHGVISEEWGGENLFAYVSAKSGQGIDGLLDQILVQAEVLELT
                     AAVDGPAKGVVIESRLDKGRGPVATLLVQSGTLRKGDIILTGLEYGRVRAMLDETGRP
                     IDEAGPSIPIEVLGLSGAPNAGDEAVVVSDERKAREIALFRQGKFREVKLARQQKAKL
                     ENVFSQMAEGEIATLNVVLKTDVQGSAEALSEALTKLSTDEVRVRIISSGVGAINESD
                     ANLAVASEAVMIGFNVRADSAAKKVIEEEGVDLHYYSVIYDVIDEIKKALVGMLSPEV
                     KEQIIGLVQVRQVFRHPKFGAIAGCMVVDGVVKRSAPIRVLRENVVIFEGELDSLRRF
                     KDDVAEVRQGFECGIGVRNYNDVREGDQIEVFERIEVQRTL"
     gene            1500..1907
                     /gene="rbfA"
                     /locus_tag="PLQ18_10470"
     CDS             1500..1907
                     /gene="rbfA"
                     /locus_tag="PLQ18_10470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008373830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HNE02121.1"
                     /translation="MAKEFSRTRRLGEQVRRDLAALMRSEIDDRRVAMVSITQVEISR
                     DLAHAKVWVTYLGPEAERPAVLKQLAEHAGLLRQQLGRGLHVRTVPRLAFVYDESIEH
                     GAKLDALIRQAVAEDAARHPDDADGHPGDDDPA"
     gene            1904..>2434
                     /gene="truB"
                     /locus_tag="PLQ18_10475"
     CDS             1904..>2434
                     /gene="truB"
                     /locus_tag="PLQ18_10475"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018953536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HNE02122.1"
                     /translation="MSSRETQPRVRRGRRDVHGILLLDKPRGLSSNAALQAVKWLYAA
                     RKAGHGGSLDPLATGLLPCLFGEATKVSGLLLDAGKRYRFTARLGVTTTTGDAEGALL
                     AERPLPPIDAGVLETVFTRFRGPIEQVPPMHSALKRDGRPLYELARQGIEVERAPRSV
                     TIHSLQLLDWSAGSFSA"
BASE COUNT          404 a          847 c          840 g          343 t
ORIGIN      
        1 gccacgtcga ccacggcaag acctcgctgc tcgactacat ccgccgcacc aaggtcgcgg
       61 ccggcgaggc cggcggcatc acgcagcaca tcggcgccta ccacgtcgag accggcaagg
      121 gcacgatcac cttcctcgat acccccggcc acgcggcgtt cacggcgatg cgtgcccgcg
      181 gtgcgaaggc gaccgacatc gtcgtgctcg tggtcgcggc cgacgacggc gtcatgccgc
      241 agacggtcga ggcgatccag cacgcgcgcg ctgccggcgt gccgatcgtc gtggccgtca
      301 acaagatgga caagaacggc atcgatctcg aacgcgtcaa gagcgagctg tcgcagcacg
      361 gcgtgatctc ggaagagtgg ggtggcgaga acctcttcgc ctacgtgtcg gcgaagtccg
      421 gacagggcat cgacggcctg ctcgaccaga tcctcgtgca ggccgaggtg ctggagctga
      481 ccgccgccgt cgacggcccg gccaagggcg tggtcatcga gtcgcgcctc gacaagggcc
      541 gcggcccggt cgcgacgctg ctggtgcagt ccggcacgct gcgcaagggc gacatcatcc
      601 tgaccggcct cgaatacggc cgcgtgcgcg cgatgctcga cgagaccggc cggccgatcg
      661 acgaggccgg tccgtcgatt ccgatcgagg tgctcggcct ctccggcgcc ccgaacgccg
      721 gtgacgaggc ggtcgtggtc agcgacgagc gcaaggcgcg cgagatcgcg ctgttccgcc
      781 agggcaagtt ccgcgaggtc aagctggcgc gccagcagaa ggccaagctc gagaacgtct
      841 tcagccagat ggccgagggc gagatcgcga cgctcaacgt cgtgctgaag accgacgtgc
      901 agggctcggc cgaggcgctg tccgaggcgc tgaccaagct gtcgaccgac gaggtgcgcg
      961 tgcgcatcat ttcgtccggc gtcggcgcga tcaacgaatc ggatgccaac ctcgccgtcg
     1021 cctccgaggc ggtgatgatc ggcttcaacg tccgtgccga cagcgcggcg aagaaggtca
     1081 tcgaggagga aggcgtcgac ctgcactact acagcgtcat ctacgacgtc atcgacgaga
     1141 tcaagaaggc gctggtcggc atgctctcgc ccgaggtcaa ggagcagatc atcggcctcg
     1201 tgcaggtgcg tcaggtgttc cgccatccga agttcggcgc gatcgccggc tgcatggtcg
     1261 tcgacggcgt ggtcaagcgc tcggcgccga tccgcgtgct gcgcgagaac gtcgtcatct
     1321 tcgagggcga gctcgactcg ctgcgccgct tcaaggatga cgtcgccgag gtccgccagg
     1381 gtttcgagtg tggtatcggc gtgcgcaact acaacgacgt gcgcgaaggc gaccagatcg
     1441 aggtcttcga gcgcatcgaa gtccagcgca cgctgtaagc gcaacggagc ccgcagccga
     1501 tggccaagga gttctcgcgt acccgccggc tcggcgaaca ggtgcgccgt gatctcgccg
     1561 cgctgatgcg cagcgagatc gacgaccggc gcgtggcaat ggtctcaatc acgcaggtcg
     1621 agatcagccg cgacctcgcg cacgccaagg tctgggtcac ctacctcggc cccgaggccg
     1681 agcgtccggc cgtgctgaag cagctcgccg agcacgccgg tctgctgcgc cagcagctcg
     1741 ggcgcggcct gcacgtgcgc accgtgccgc ggctcgcctt cgtctacgac gaatcgatcg
     1801 agcacggcgc gaagctcgat gcgctgatcc gtcaggcggt cgccgaagac gcggcccgcc
     1861 accccgacga cgcggacggt catcccggcg acgacgatcc ggcatgagtt cccgtgaaac
     1921 ccagccgcgc gtgcgccggg gacggcgcga cgtgcatggc atcctgctgc tcgacaagcc
     1981 ccgcggcctg tcctcgaacg ccgcgctgca ggcggtgaaa tggctgtacg cggcccgcaa
     2041 ggccgggcac ggcggcagcc tcgatccgct cgccaccggc ctgctgccct gcctgttcgg
     2101 cgaggcgacc aaggtctcgg gcctgctgct cgacgccggc aagcgctatc gcttcacggc
     2161 caggctcggc gtcaccacca cgaccggcga tgccgaaggc gcgctgctcg ccgagcggcc
     2221 gctgccgccg atcgatgccg gcgtgctcga aaccgtgttc acgcgctttc gcggcccgat
     2281 cgaacaggtg ccgccgatgc attcggcgct gaagcgcgac ggccggccgc tgtacgagct
     2341 cgcccgccag ggcatcgagg tcgagcgcgc cccgcgcagc gtcacgatcc acagcctgca
     2401 gctgctcgac tggagcgccg ggagcttctc ggcc
//
LOCUS       DAOQJH010000472         2429 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_31916_length_2429_cov_4.194608, whole genome shotgun sequence.
ACCESSION   DAOQJH010000472 DAOQJH010000000
VERSION     DAOQJH010000472.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2429)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2429)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2429
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31916_length_2429_cov_4.194608"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..381)
                     /locus_tag="PLQ18_10480"
     CDS             complement(<1..381)
                     /locus_tag="PLQ18_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019556461.1"
                     /note="has catalase and peroxidase activities; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase-peroxidase"
                     /protein_id="HNE02123.1"
                     /translation="MSESQCPFHHTSGGGTSNRDWWPNQLNLDILRQHAGKSSPTGAG
                     FDYAKAFAGLDYAALKQDLHALMTDSQDWWPADYGHYGPMFIRMAWHSAGTYRTGDGR
                     GGAGSGSQRFAPLNSWPDNGNLDKA"
     gene            790..1212
                     /locus_tag="PLQ18_10485"
     CDS             790..1212
                     /locus_tag="PLQ18_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNE02124.1"
                     /translation="MHAHLTMITLAVEDLERAVQFYRDGLGLPTDGIVGTEFEHGAVA
                     FFELAGGLALALWPRASLAHETGSVAGTPGTTDFALAHNVTTIEEVDALLAQAQAAGA
                     QIVRPGAPAFWGGYTGWFRDPDGHLWEIAWNPSRLPQA"
     gene            complement(1223..2164)
                     /locus_tag="PLQ18_10490"
     CDS             complement(1223..2164)
                     /locus_tag="PLQ18_10490"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNE02125.1"
                     /translation="METGKTSGGLPPDFEGLHACIDHLPGNVMLADAGFILRYANRAA
                     LRTLRLLEPEIQRVFGVRFEQLLNGSIHRFHREPERIERLLRDYVQTLPREASFTFGS
                     VTLRTHINAVLSDGRLLGYCVIWDDISRESALEAEIALSRERAGQLAADLLHRLSELR
                     LSVEEIARTCTQVVEVATRGRVASDLAASSVGRLGQQGEAIGGVLDLIGKVAEQTKLL
                     ALNAGIEASRAGEAGRGFAVVAREIKDLAAATAGATGDIAKRIGTIRDGVEVTVEAIE
                     ALRRIIDELHHLQTQVAAAVEEQSAVVAELERARLQP"
BASE COUNT          350 a          853 c          811 g          415 t
ORIGIN      
        1 cgccttgtcg aggttgccgt tgtcgggcca gctgttcagc ggcgcgaagc gctggctgcc
       61 gctgccggca ccgccgcggc cgtcgccggt gcggtaggtg ccggcgctgt gccaggccat
      121 gcggatgaac atcggtccgt aatggccgta atccgccggc caccagtcct gcgaatcggt
      181 catcagcgca tgcaggtcct gcttcagggc cgcgtaatcc agaccggcga aggccttggc
      241 gtagtcgaaa ccggctccgg tcggactgga cttgccggcg tgctggcgca ggatgtccag
      301 attcagctga ttgggccacc agtcacgatt cgacgtgccg ccaccggagg tgtgatggaa
      361 cgggcattgg ctttcggaca tctcgtcatc tccacttgcg ttctggtttc gtactgcggg
      421 tccggcccgg tctgtatagc cgcccgccca ccggtgcgac caattgaacc tgccaaagcc
      481 cgcgatagcc tgccgcgatg gttgatgcgg gatgacgccc gaatgccgct gcggcccgca
      541 gcgatggatc acggcagatg ccggttccgg taataccttc cgtccgatgc cgattgccgg
      601 cacggccggg tttcagaatt cctgcatttc accgcgaaaa cttcatgctt tccggccgcc
      661 aatcccgtcc ggccggctga aaacggcttc tgcgcgcagg accggcatgc ggattctccc
      721 ggcatgacga cgcacgtcct gccatattca gaggtcgagg gctgcaggcc ccgaacggag
      781 accgccgcca tgcatgcaca cctgacgatg atcacgctgg ccgtcgagga tctcgaacgc
      841 gccgtgcagt tctaccgcga cggcctcggc ctgccgaccg atggcatcgt cggcaccgag
      901 ttcgagcacg gtgccgtcgc gttctttgaa ctggccggcg gcctggcgct ggcgctgtgg
      961 ccgcgcgcga gtctggccca tgaaaccggc agcgtcgccg gaacgcccgg cacgacggat
     1021 ttcgcgctcg cgcacaacgt cacgaccatc gaggaggtcg atgcgctgct cgcgcaggcg
     1081 caggcggccg gcgcgcagat cgtccggccg ggcgcgccgg ccttctgggg cggttacacc
     1141 ggctggttcc gcgatccgga cgggcatctg tgggagatcg cgtggaaccc gtcacggctg
     1201 ccgcaggcct gatccgcccg gctcacggct gcaggcgcgc gcgctccagc tcggcgacca
     1261 ccgccgactg ctcctcgacc gcggctgcca cctgcgtctg caggtgatgc agctcgtcga
     1321 tgatccggcg cagcgcctcg atcgcttcga cggtcacctc gacgccgtca cggatcgtgc
     1381 cgatgcgctt ggcgatgtcg ccggtcgcgc cggcggtcgc ggcggcaaga tccttgatct
     1441 cgcgcgcgac caccgcgaag ccgcgtccgg cctcgccggc gcgcgaagcc tcgatgccgg
     1501 cgttgagcgc cagcagcttg gtctgctcgg cgaccttgcc gatcaggtcg agcacgcccc
     1561 cgatcgcctc gccctgctgg ccgagccgcc cgaccgaact ggcggccagg tcgctggcca
     1621 cgcgcccgcg ggtcgcgacc tcgacgacct gcgtgcaggt gcgtgcgatc tcctcgacgc
     1681 tgaggcgcag ctcggagagc cggtgcagca ggtcggcggc gagctggccg gcgcgttcac
     1741 gactcagggc gatttccgcc tccagcgcgg attcgcggct gatgtcgtcc cagatcacgc
     1801 agtaccccag cagccggccg tcgctgagca cggcattgat gtgcgtgcgc agcgtgacgc
     1861 tgccgaacgt gaagctcgcc tcacgcggca gcgtctgcac gtagtcgcgc agcagccgct
     1921 cgatgcgctc gggctcgcgg tggaagcgat ggatcgagcc gttcagcaac tgctcgaagc
     1981 gcacgccgaa gacccgctgg atttcaggtt cgagcagccg cagcgtgcgc agcgccgcgc
     2041 ggttggcata gcgcagaatg aaaccggcat ccgccagcat gacgttgccg ggcaggtgat
     2101 cgatgcaggc atgcagcccc tcgaaatccg gaggcagccc tccggaggtc tttccggttt
     2161 ccatgtcgta taccccttct cgctttattg gtctgacctg tctggcctgt atggctggcc
     2221 cggcctgtcc ggccagacct gcccgtgcgt ctggaaacca gcctccgccg gcccggatgc
     2281 acgatgtgga cggcaccggt cgctccggtc gccgctgatc atgttcccct gccggcccgg
     2341 ggccgtgcag cctgttaccg ccatttcatc gcccccggat cggcgcgcac gccggcgacg
     2401 cttcgcgtta ggctgcgcga ccgatccgc
//
LOCUS       DAOQJH010000473         2421 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_32100_length_2421_cov_4.139899, whole genome shotgun sequence.
ACCESSION   DAOQJH010000473 DAOQJH010000000
VERSION     DAOQJH010000473.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2421)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2421)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2421
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32100_length_2421_cov_4.139899"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1718
                     /locus_tag="PLQ18_10495"
     CDS             <1..1718
                     /locus_tag="PLQ18_10495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007588603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysine decarboxylase"
                     /protein_id="HNE02126.1"
                     /translation="MFHQFFGENMLRADVCNAVEELGQLLDHTGPVAASERNAARIFN
                     ADHLFFVTNGTSTSNKIVWHSTVAPGDIVVVDRNCHKSILHAITMTGAIPVFLTPTRN
                     HYGIIGPIPLKAFKPDNIRKKIEANPFARAALEKHPDRKPRILTLTQSTYDGVLYNVE
                     MIKDMLGDTVDNLHFDEAWLPHAAFHPFYRDMHAIGTDRPRSKQALVFATHSTHKLLA
                     GLSQASQIVVQDSEAKKLDRHRFNEAYLMHTSTSPQYAIIASCDVAAAMMEPPGGTAL
                     VEESISEALEFRRAMRAVEEEFDADSWWFKVWGPDRLAEEGIGEREDWILRPEDRWHG
                     FAKLTDGYNMLDPIKATIITPGLDVDGSFAERGIPAAIVTKYLAEHGIIVEKTGLYSF
                     FIMFTIGITKGRWNTLVTELQQFKDDYDRNQPLWRVLPEFVARFPQYERIGLRDLCRQ
                     IHGVYRDNDIARLTTEMYVSNIEPAMKPTDAFAAQVHEEIDRVPIDELEGRITSILLT
                     PYPPGIPLLIPGERFNRTIMRYLQFARDFNARFPGFETDIHGLVTEDVDGVLHYYVDC
                     VQQSG"
     gene            1829..>2421
                     /locus_tag="PLQ18_10500"
     CDS             1829..>2421
                     /locus_tag="PLQ18_10500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003395611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNE02127.1"
                     /translation="MLSTANLTMQFGPKPLFENVTVKFGAGNRYGLIGANGCGKSTLM
                     KILGGDLESTNGIVMREPNTRLGKLRQDQFAYENLSVIDTVILGHEELAAVKTERDAI
                     YALPEMSEDDGMRVADLEVRFAELDGYTAEARAGELLLGLGIEEALHWGPMSAVAPGW
                     KLRVLLAQALFSDPDVLLLDEPTNHLDINTIRWLEDV"
BASE COUNT          448 a          848 c          748 g          377 t
ORIGIN      
        1 agatgttcca ccagttcttc ggcgagaaca tgctgcgtgc cgacgtctgc aacgccgtcg
       61 aggaactcgg ccagctgctc gatcacaccg gcccggtcgc ggccagcgag cgcaatgccg
      121 cgcgcatctt caatgccgat cacctgttct tcgtgaccaa cggcacctcg acctcgaaca
      181 agatcgtctg gcactcgacg gtcgcgcccg gcgacatcgt cgtcgtcgac cgcaactgcc
      241 acaaatccat cctgcacgcg atcacgatga ccggcgcgat cccggtgttc ctgacgccga
      301 cgcgcaatca ctacggcatc atcggtccga tcccgctgaa ggcgttcaag cccgacaaca
      361 tccgcaagaa gatcgaggcc aacccgttcg cgcgcgccgc gctcgaaaag cacccggacc
      421 gcaagccgcg catcctgacg ctgacgcagt cgacctacga cggcgtgctc tacaacgtcg
      481 agatgatcaa ggacatgctc ggcgacaccg tcgacaacct gcatttcgac gaggcctggc
      541 tgccgcacgc ggcgtttcat ccgttctacc gcgacatgca cgcgatcggc accgaccggc
      601 cgcgctcgaa gcaggcgctg gtgttcgcga cgcattcgac gcacaagctg ctcgccggcc
      661 tgtcgcaggc ctcgcagatc gtcgtgcagg attccgaggc caagaagctc gaccgccacc
      721 gcttcaacga agcctacctg atgcacacct cgacctcgcc gcagtacgcg atcatcgcga
      781 gctgcgacgt ggctgcagcg atgatggaac cgccgggcgg cacggcgctg gtcgaggaat
      841 cgatcagcga ggcgctcgaa ttccgccgcg cgatgcgcgc ggtcgaggag gaattcgacg
      901 ccgattcgtg gtggttcaag gtctggggac cggaccggct ggccgaggag ggcatcggcg
      961 agcgcgagga ctggatcctg cgccccgagg accgctggca cggcttcgcg aagctgaccg
     1021 acggctacaa catgctcgac ccgatcaagg cgacgatcat cacgcccggg ctcgatgtcg
     1081 acggcagctt cgccgagcgc ggcatcccgg cggcgatcgt gaccaagtat ctggccgagc
     1141 acggcatcat cgtcgagaag accgggctgt actcgttctt catcatgttc acgatcggca
     1201 tcaccaaggg ccgctggaac acgctggtca cggagctgca gcagttcaag gacgactacg
     1261 accgcaacca gccgctctgg cgcgtgctgc cggagttcgt cgcgcgcttc ccgcagtacg
     1321 agcgcatcgg cctgcgggat ctctgccggc agattcacgg cgtctaccgc gacaacgaca
     1381 tcgcgcgcct gaccaccgag atgtacgtct cgaacatcga gccggcgatg aagccgaccg
     1441 atgccttcgc cgcccaggtg cacgaggaga tcgaccgcgt gccgatcgac gagctcgaag
     1501 gccgcatcac cagcatcctg ctgacgccgt acccgcccgg catcccgctg ctgattccgg
     1561 gcgaacgctt caaccgcacg atcatgcgct acctgcagtt cgcgcgggat ttcaatgccc
     1621 gcttcccggg cttcgagacc gacatccacg gcctcgtgac cgaggatgtc gacggcgtgc
     1681 tgcattacta cgtggactgc gtgcagcagt cgggctgaag caccgttccc gcctgccatc
     1741 gccggcccgc gcgtgcgggc cggcgatttt tcatttatgg ctttcccctg atccatgcgc
     1801 gcccgccgcg ccgcgagtga ccgacacgat gctttccacc gccaacctca cgatgcagtt
     1861 cgggccgaag cccctgttcg agaacgtcac cgtcaagttc ggagccggca accgctacgg
     1921 cctgatcggc gccaacggct gcggcaagtc gacgctgatg aagatcctcg gcggcgatct
     1981 ggagtcgacc aacggcatcg tcatgcgcga gccgaacacg cgcctcggca agctgcgcca
     2041 ggaccagttc gcctacgaga acctgagcgt catcgacacc gtgatcctcg gccacgagga
     2101 gctggcggcg gtgaagaccg agcgcgacgc gatctacgcg ctgcccgaga tgagcgagga
     2161 cgacggcatg cgcgtcgccg acctcgaagt gcgcttcgcc gaactcgacg gctacaccgc
     2221 cgaggcgcgc gccggcgagc tgctgctcgg cctcggcatc gaggaggccc tgcactgggg
     2281 gccgatgagc gcggtcgcgc cgggctggaa gctgcgcgtg ctgctcgcgc aggcgctgtt
     2341 ctcggacccc gacgtgctgc tgctcgacga gccgaccaac cacctcgaca tcaacaccat
     2401 ccgctggctg gaagacgtga t
//
LOCUS       DAOQJH010000474         2419 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_32143_length_2419_cov_3.661168, whole genome shotgun sequence.
ACCESSION   DAOQJH010000474 DAOQJH010000000
VERSION     DAOQJH010000474.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2419)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2419)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2419
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32143_length_2419_cov_3.661168"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..507)
                     /locus_tag="PLQ18_10505"
     CDS             complement(<1..507)
                     /locus_tag="PLQ18_10505"
                     /inference="COORDINATES: protein motif:HMM:NF027806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HNE02128.1"
                     /translation="MRESTLIFLLLLSAALALQIWLALRQLRHVRAHREQVPPAFAAD
                     IPLAAHRRAADYTLERTRFGLLALVVDTLVLLGWTLGGGFAALDAAWRGFGWGETATG
                     VAVIGSAIAIGSLLVLPLKLWRVFVIEARHGFNRTTPALFASDLVKGSLLGAALGLPL
                     LALALWLMA"
     gene            602..1150
                     /gene="orn"
                     /locus_tag="PLQ18_10510"
     CDS             602..1150
                     /gene="orn"
                     /locus_tag="PLQ18_10510"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007161168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-exoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HNE02129.1"
                     /translation="MTPNPEHLIWIDLEMTGLDPDSDCIIEIATIVTDNALNVLAEGP
                     VFAIHRSDDVLNGMDEWNTRQHAKSGLSARVRASSISAAAAEQATLDFLAGWVPKGKS
                     PMCGNSICQDRRFLARGMPALEAWFHYRNLDVSTLKELGKRWFPERVKGFQKGNAHLA
                     LDDIRESIAELRFYRERLFVSD"
     gene            1480..1878
                     /locus_tag="PLQ18_10515"
     CDS             1480..1878
                     /locus_tag="PLQ18_10515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02130.1"
                     /translation="MKAHIVPAALLLACAAALAACDDGQRPATSATQTTTEQKTETAK
                     ESAAAAASAAGEAVRHAGAALGAGASAAKDVTVEAAREAADKLREGYDATTQAAGRAV
                     ERGREALRDEPVGTRTGTTPADDTATRTTN"
     gene            complement(2165..>2419)
                     /locus_tag="PLQ18_10520"
     CDS             complement(2165..>2419)
                     /locus_tag="PLQ18_10520"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNE02131.1"
                     /translation="AINLDWSTAFDPGRRPRTKPIEHLRAALLAAGVDPAREIVVYCQ
                     THHRSAHTWVVLKALGYPRVRGYAGAWSEWGNTPGLPIEP"
BASE COUNT          399 a          889 c          836 g          295 t
ORIGIN      
        1 cgccatcagc cacagcgcca gcgccagcag cggcaggccg agcgcggcgc caagcagcga
       61 gcccttgacc agatcgctcg cgaacagcgc cggcgtcgtg cggttgaagc cgtggcgcgc
      121 ctcgatcacg aacacccgcc agagcttcag cggcagcacc agcaggctgc cgatcgcgat
      181 cgccgagccg atcaccgcga cgccggtcgc ggtctcgccc cagccgaagc cgcgccaggc
      241 cgcatcgagc gccgcgaagc cgccgccgag cgtccagccg agcagcacca gcgtgtcgac
      301 gaccagcgcc agcaggccga agcgcgtgcg ttcgagcgtg tagtcggccg cgcgccggtg
      361 cgcggcgagc ggaatgtcgg ccgcgaaggc cggcggcacc tgctcgcggt gggcgcgtac
      421 gtggcgcaac tggcgcagcg ccagccagat ctgcagcgcc agcgccgcgg acagcaacag
      481 caggaaaatc agcgtagact cgcgcattcg ccggaaaggc cgtgtgggac accggtcccg
      541 agcgtagccc gcgccccgcg cacgcggcaa ccggcccctg cccccgccga gaagccgcgc
      601 catgacgccc aatcccgagc acctgatctg gatcgatctg gagatgaccg gactcgatcc
      661 ggacagcgac tgcatcatcg agatcgccac gatcgtcacc gacaacgcgc tgaacgtgct
      721 ggccgaaggc ccggtgttcg cgatccaccg ctccgacgac gtgctgaacg gcatggacga
      781 gtggaacacg cgccagcatg cgaagtcggg gctgagcgcg cgcgtgcgcg ccagcagcat
      841 cagcgccgcc gcggccgagc aggcgacgct cgatttcctc gccggctggg tgccgaaggg
      901 caagtcgccg atgtgcggca acagcatctg ccaggaccgg cgcttcctcg cgcgcggcat
      961 gccggcgctg gaggcgtggt tccactaccg caacctcgac gtgagcacgc tgaaggagct
     1021 tggcaagcgc tggttcccgg agcgcgtcaa gggcttccag aagggcaacg cccacttggc
     1081 gctcgatgac atccgcgaat cgatcgccga actgcgcttc taccgcgagc gtctgttcgt
     1141 gagcgactga acgatccgcg cccgcctgca aggcgctgtc tccacggcgg ccggcccggc
     1201 aacggaccgg ccgccggcgt tttggcgtcg ccggcaggcg acaactcaag cccttcgtgc
     1261 ggcacgcttc cgccgcgccc gcccggcaac tgtcgccgtt gcccgacggt ttgcgcacgc
     1321 tcgtggcgct ccggcgccgg cagcggacgg gcattccgcc ctcaagcgcc tgcaagcacc
     1381 tgatcgccgg cggatttccc ggacatggca cgaaatcgcg cattcacggt ccatgcaagg
     1441 caggtcgagc cctgcccatc ctggtgaccg gaacctctca tgaaagcgca catcgtcccc
     1501 gccgccctgc tgctcgcctg cgccgccgcg ctcgccgcct gcgacgacgg ccagcgtccg
     1561 gccacatcgg ccacacaaac caccaccgaa cagaaaaccg aaaccgcgaa ggaatcggct
     1621 gcagccgcgg cctcggccgc cggcgaggcg gtccgccacg ccggtgccgc gctcggtgcc
     1681 ggtgccagcg ccgcgaagga cgtgaccgtc gaagccgcgc gcgaggccgc cgacaagctg
     1741 cgcgaaggtt acgacgcgac cacgcaggcc gccggccgcg ccgtcgagcg cgggcgcgag
     1801 gctctgcgtg acgagccggt cgggacccgc accggcacga cgccggccga tgacacggcc
     1861 acacggacga cgaactgaaa gcccgcaggc ctgacggacg ggtcagcctg aacgggaggt
     1921 ttttccccca ccggcccgca tggaacgcga gccggctgat gcgaccggag ctgacgcgca
     1981 ggctgcgcgg gtcgcaacgg aaccgcggac cggattccgg gtgaatgagg acggttcata
     2041 cgcattcccc gtgcgagggg atggtggtcg accgaccggg ctgcgggtcc cggccggtcg
     2101 ttttttttgt gccgctcccg tcatgcgagc agagcgcagg ccggcctcgc gccgggcggc
     2161 gtgctcaggg ttcgatcggc agtcccggcg tgttgcccca ctccgaccag gcgccggcat
     2221 agccgcgcac gcgcgggtag ccgagtgcct tcagcaccac ccaggtgtgc gccgagcggt
     2281 gatgcgtctg gcagtagacg acgatctcgc gcgccggatc gacgccggcg gccagcagcg
     2341 ccgcgcgcag gtgctcgatc ggcttggtgc gcggccgccg gcccggatcg aaggccgtgc
     2401 tccagtcgag gttgatcgc
//
LOCUS       DAOQJH010000475         2418 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_32167_length_2418_cov_3.495556, whole genome shotgun sequence.
ACCESSION   DAOQJH010000475 DAOQJH010000000
VERSION     DAOQJH010000475.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2418
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32167_length_2418_cov_3.495556"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..250)
                     /locus_tag="PLQ18_10525"
     CDS             complement(<1..250)
                     /locus_tag="PLQ18_10525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02132.1"
                     /translation="MNAVVRPPETARRHAGELELRAPWYAAASDGCTLHRYLDDDFVP
                     RFLGELAAGGPADDGWTQADRFGGSALPVLRLPVHRAFY"
     gene            complement(255..911)
                     /locus_tag="PLQ18_10530"
     CDS             complement(255..911)
                     /locus_tag="PLQ18_10530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HNE02133.1"
                     /translation="MAAEPQQHALAGAVGTLQLAIVTDTRDPENRGRVQVRLVALEVE
                     LWAPVLVASAGNGYGVACLPKIGEIVALGFVGPELPLVLGSLWTGAAPPQPGAAPVEQ
                     RYSVKTPAGTELRFDDAGSPQLLIETPAGQRLRLSDSGNGLLRIEIAGNTITVDGSSV
                     RVQAVEEISLSAPQVNISAGQLKVAAGMSQFSGVVKCDTLISNAVVSASYTPGAGNVW
                     "
     gene            complement(901..1935)
                     /locus_tag="PLQ18_10535"
     CDS             complement(901..1935)
                     /locus_tag="PLQ18_10535"
                     /inference="COORDINATES: protein motif:HMM:NF017739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="contractile injection system protein, VgrG/Pvc8
                     family"
                     /protein_id="HNE02134.1"
                     /translation="MSDSAFTAARPWCRIDGSLRADVQTAVLACTAGGRIGEPAHAEV
                     RLLNWGGRGGADAGFLFDSLQPGQTLTLGFGDDEVFTGDITAVEERYGGGAPQLVLLA
                     EDALHRLARSRHSRSFEALSPAALLTQLAAAARLQADCNVDAPVATWHQLNESDLALL
                     CRVLGRYDIEPWLDGTKLVARRPEAPTGTLALNAMDSAELRLIADLAQQPTAVEVRGY
                     DLASGSEVRGSARTLQPPPGAVTAAARLAALGWPGAEVFPQPAAGSQTDADGLAAAAF
                     GRRAGAWVHGDMRLRGNPKLRRGGYVELSGVSKRFAGRYRMTGVWQSFDLTEGFRTRL
                     KLERGDWNGS"
     gene            complement(1960..2130)
                     /locus_tag="PLQ18_10540"
     CDS             complement(1960..2130)
                     /locus_tag="PLQ18_10540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02135.1"
                     /translation="MPVHIEELDTTMTEPPPAPANDAQAETDARPVQPDPLLLALALE
                     LLAERRARLQFD"
     gene            complement(2202..>2418)
                     /locus_tag="PLQ18_10545"
     CDS             complement(2202..>2418)
                     /locus_tag="PLQ18_10545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02136.1"
                     /translation="TAGVGVGVGAGVGVGAGVDAGVGVFVPPGPAPSLGGGFTFGASA
                     RLGTHLGAAVETPAPAVRAGVSIRFGV"
BASE COUNT          324 a          864 c          903 g          327 t
ORIGIN      
        1 cgtagaacgc gcggtgcacc ggcaggcgca gcaccggcag cgcactgccg ccgaagcggt
       61 cggcctgcgt ccagccgtca tcggccggtc cgccggcggc gagttcgccg aggaaacgcg
      121 ggacgaagtc gtcatcgagg tagcggtgca gcgtgcagcc atcgctcgcc gctgcgtacc
      181 acggcgcgcg cagttcgagt tcgccggcgt ggcggcgggc ggtctcgggc ggacggacga
      241 cggcgttcat cgcttcacca gacgttgccg gcgcccggcg tgtagctcgc cgagaccacc
      301 gcgttgctga tcagcgtgtc gcacttgacc acgccgctga actggctcat gccggccgcg
      361 accttgagct gtccggcgct gatgttgacc tgcggggcgc tgaggctgat ctcttccacg
      421 gcctgcacgc gcacgctgct gccatcgacg gtgatcgtgt tgccggcgat ctcgatgcgc
      481 agcaggccgt tgccgctgtc gctcaggcgc aggcgctggc cggccggggt ctcgatcagc
      541 agctgcgggc tgccggcgtc atcgaagcgc agctcggtgc cggccggcgt cttcacgctg
      601 tagcgctgct cgaccggcgc ggcgccgggc tggggcgggg ctgcgccggt ccacaggctg
      661 ccgagcacca gcggcagctc cgggccgacg aagccgagcg cgacgatctc gccgatcttc
      721 ggcaggcagg cgacgccgta gccgttgccg gccgaggcca cgagcaccgg cgcccacagc
      781 tcgacctcga gcgcaacgag gcgcacctgc acgcggccgc ggttctcggg gtcgcgcgtg
      841 tcggtgacga tcgccagttg cagcgtgccg acggcaccgg cgagggcgtg ctgctgcggt
      901 tcagctgcca ttccagtccc cgcgttcgag cttcagccgc gtgcggaagc cttcggtcag
      961 atcgaaggac tgccagacgc cggtcatccg gtagcgcccg gcgaagcgct tcgacacgcc
     1021 ggacagttcc acgtagccgc cgcggcgcag cttcgggttg ccgcgcagcc gcatgtcgcc
     1081 gtgtacccag gcgccggcac ggcggccgaa cgcagcggcg gcgaggccgt cggcgtcggt
     1141 ctgtgagccg gcggccggct gcgggaagac ctcggcaccg ggccagccga gtgcggccag
     1201 ccgcgcggct gcagtcaccg cgccgggcgg gggctgcagc gtgcgtgccg agccccggac
     1261 ttcgctgccg ctcgcaaggt cgtagccgcg tacctcgacc gcggttggct gctgcgcgag
     1321 gtcggcgatc aggcgcagct cggcgctgtc catcgcattg agtgcgagcg tgccggtcgg
     1381 cgcttcgggg cggcgggcga cgagcttcgt gccatcgagc cagggctcga tgtcgtagcg
     1441 tccgagcaca cggcagagca gcgctaggtc gctttcgttg agctgatgcc aggtcgcgac
     1501 cggcgcatcg acgttgcagt cggcctgcag ccgggcggca gcggcgagct gcgtgagcag
     1561 cgccgccggc gacagcgcct cgaagctgcg gctgtggcga ctgcgtgcca gccggtgcag
     1621 cgcgtcttcg gcgagcagca cgagttgcgg cgcgccgccg ccgtagcgtt cctcgactgc
     1681 ggtgatgtcg ccggtgaaaa cctcgtcgtc gccgaagccg agcgtcagcg tctggcccgg
     1741 ctgcagcgaa tcgaacagga aaccggcatc ggcaccgcca cgcccgcccc agttcagcag
     1801 cctgacttcg gcgtgcgcgg gctcgccgat gcggccaccg gccgtgcagg cgagcacggc
     1861 cgtctgcacg tcggcgcgca gactgccgtc gatgcggcac cacgggcggg cggcggtgaa
     1921 ggcggagtca gacatcggcc gggttcctgt accggggcat cagtcgaact gcagccgggc
     1981 ccggcgctcg gcgagcagtt cgagcgcgag cgcgagcagc agcggatcgg gctgcaccgg
     2041 gcgcgcgtcg gtctcggcct gcgcgtcgtt cgccggcgcc ggcggcggct cggtcatggt
     2101 cgtgtcgagt tcctcgatat gcaccggcat cgcccggact cctgaaagtg ttcgccagag
     2161 tgcttgccag aaagcccgcc aaagtgcccc ccggacgccg ctcagacgcc gaagcggatc
     2221 gacacgccgg cgcgcacggc cggtgccggg gtttccaccg cggcgccgag atgcgtgccg
     2281 agccgggccg aggcgccgaa cgtgaagccg ccgccgagcg acggtgccgg acccggcggc
     2341 acgaacacgc cgaccccggc atcgacgccg gccccgacgc cgactccagc cccgaccccg
     2401 acgccgactc cggccgtg
//
LOCUS       DAOQJH010000476         2410 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_32366_length_2410_cov_5.789384, whole genome shotgun sequence.
ACCESSION   DAOQJH010000476 DAOQJH010000000
VERSION     DAOQJH010000476.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2410)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2410)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2410
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32366_length_2410_cov_5.789384"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..280)
                     /locus_tag="PLQ18_10550"
     CDS             complement(<1..280)
                     /locus_tag="PLQ18_10550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02137.1"
                     /translation="MELTEAEIAMLPLVVRQQYEADLAASRRAADEQAAKQPETDRPE
                     EIAPENPAPAAPVASDDGLRHRFDVLQGKYNAEVPRLHDELRATRSENE"
     gene            complement(375..590)
                     /locus_tag="PLQ18_10555"
     CDS             complement(375..590)
                     /locus_tag="PLQ18_10555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02138.1"
                     /translation="MLSKPDERVTRALAILARDAQFQCLREWLEAERARLAQAMSAER
                     SDVELRMQQGAAQTLSRILDAAERAAS"
     gene            complement(574..>2410)
                     /locus_tag="PLQ18_10560"
     CDS             complement(574..>2410)
                     /locus_tag="PLQ18_10560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02139.1"
                     /translation="AKQPHERRMLDALRRRKGEYDPAKLAAIRAAGGSEIFIGITGQK
                     CRDAEAWIRDIDMPAGETPWAIDPSPVPELPPDEIEEIRREVLTRAQAIMASGMPLEM
                     SPEDYQERIADRMRGEMQEEAKERARRMADLVETQMQAAKWNRVFRAFVGDLVTFPAA
                     CIRGPVIRRRKRMAWVQDGADWRADVADELRPEFDRVSPLDLYPSADATDMARSYMCE
                     VMSMTRSDLAAMIGVPSFSEAAVRAALDEYDRGGARGDWLAYNDSERERVEGRDSARA
                     GSDTDTMRALSYWGSVPGKWLTEWGLAGITDPQAEYQVNVWLVGRHAVRAMLNDDPLG
                     RDPYHITSFARVPGSFWGSGVPDLCEDAQDGANAAARALANNVAFASGPLTEMDVSRF
                     PNGIAPPIQPWQRFEVDSYGVQRTDPAVRFYQAEMHGAELINVIEFWLDKADQVTGIP
                     RYMHGDQEATGAATTMGGLSMLMAASAKGLKDVLGNIDADVLEPAVERMIEHNNRYST
                     DPAVKGDLHARALGGNSMMMRERQAQMRSEFLDRTNNPNDMQIMGIEGRAELLREAVK
                     PLELSDPEKVVPDEDALQQRLMQQQAAQQQAAMQQQAMSANAQQA"
BASE COUNT          332 a          857 c          775 g          446 t
ORIGIN      
        1 gctcgttttc gctgcgcgtg gcgcgcaact catcgtgcag ccgcgggact tcggcgttgt
       61 acttgccctg cagcacgtcg aaccggtgcc gcagcccgtc gtcgctcgca accggagccg
      121 caggagcggg attctcgggt gcgatctctt ccgggcggtc ggtttccggc tgcttcgcgg
      181 cctgctcgtc agcagcccgg cggcttgcag ccagatcggc ctcgtactgc tggcgcacga
      241 ccagcgggag catggcgatt tccgcctcgg tcaattccat ctcgcgagcc tcgctgaggt
      301 attcgcacgg gatacggccg tctcacgacg gtctgatgta cgtcgcgagc ccatgcgggt
      361 gttcgcgacg attctcacga tgcagccctt tcggctgcgt cgagaattct ggaaagcgtc
      421 tgcgctgcgc cctgctgcat gcgcaactcc acgtcggagc gctcggcgga catggcctgc
      481 gcgagacgcg cgcgctcggc ctccagccac tcgcgcaggc actggaactg cgcgtcccgc
      541 gcgagaatcg ccagcgcgcg cgtgacgcgc tcgtcaggct tgctgagcat tcgccgacat
      601 cgcttgctgc tgcatcgcgg cctgttgctg cgccgcctgc tgctgcatca gccgctgctg
      661 cagcgcatct tcgtccggca cgaccttctc cggatccgac aactccagcg gcttgaccgc
      721 ttcgcgcagc agttcggctc ggccttcgat gcccatgatc tgcatgtcgt tcgggttgtt
      781 ggtcctgtcg aggaattcgg agcgcatctg cgcctgccgc tcgcgcatca tcatcgagtt
      841 gccgccgagc gcccgcgcat gcaggtcgcc cttgacggcc gggtcggtgc tgtagcggtt
      901 gttgtgctcg atcattcgct caacggccgg ctccagcacg tccgcgtcga tgttgccgag
      961 aacgtccttc aggcccttcg cgctcgccgc catcagcatc gacagcccgc ccatcgtcgt
     1021 ggccgcgccg gtggcctcct gatcgccgtg catgtagcgc gggatgccgg tcacctggtc
     1081 ggccttgtcc agccagaatt cgatgacgtt gatcaactcg gcgccgtgca tctcggcctg
     1141 gtagaagcgc acggccggat cggtgcgctg cacgccgtag gaatccactt cgaatcgctg
     1201 ccacggctgt atgggcggcg caatgccgtt cgggaaccgg ctgacatcca tctcggtcag
     1261 cgggccggac gcgaaggcga cgttattggc cagcgcgcgc gccgcggcat tcgcgccgtc
     1321 ctgcgcgtcc tcgcacagat caggcacgcc gctgccccag aagctgccgg ggacgcgggc
     1381 gaatgacgtg atgtggtatg ggtcgcgccc gagcggatca tcgttcagca tcgcgcgcac
     1441 cgcgtgccgg ccgaccagcc agacgttcac ctgatactcg gcctgcgggt cggtgatgcc
     1501 ggcgagaccc cattccgtca gccacttgcc cggcacactg ccccagtagg acagcgcgcg
     1561 catcgtgtcg gtgtcgctgc cggcgcgcgc tgaatcgcgg ccctcgacgc gctcgcgctc
     1621 gctgtcgttg tacgcaagcc agtcgccgcg ggcgccgccg cggtcgtact catcgagcgc
     1681 ggcgcgtacc gcagcctccg agaacgacgg cacaccgatc atggccgcca gatcggagcg
     1741 cgtcatcgac atgacttcgc acatgtagct gcgcgccatg tccgtcgcgt ccgccgacgg
     1801 gtacaggtcg agcgggctga cgcgatcgaa ctcgggtcgc agttcatcgg ccacgtctgc
     1861 gcgccagtcg gcaccgtcct gcacccaggc catgcgcttg cgccggcgga tgaccgggcc
     1921 gcggatgcac gcagccggga acgtcaccag atcgccgacg aatgcgcgga acacgcgatt
     1981 ccacttcgca gcctgcatct gggtctcgac caggtcggcc attcggcgcg cgcgctcctt
     2041 cgcctcttcc tgcatttcgc cgcgcatgcg gtcggcaatc cgctcctgat aatcctcagg
     2101 gctcatttcg agcggcatgc cggacgccat aattgcttgc gcccgcgtca ggacttcccg
     2161 ccggatttcc tcaatctcgt ccggcggcag ctccggcact ggcgacgggt cgatagccca
     2221 cggcgtttcg ccggccggca tgtcgatgtc ccgaatccat gcttcggcat cgcggcactt
     2281 ctgaccggtg atgccgatga aaatctcact gcccccggcg gcgcggatcg ccgcgagctt
     2341 ggccgggtcg tactcgccct tgcgccggcg cagcgcgtcc agcatgcgcc gctcgtgcgg
     2401 ctgcttggcg
//
LOCUS       DAOQJH010000477         2410 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_32368_length_2410_cov_4.534183, whole genome shotgun sequence.
ACCESSION   DAOQJH010000477 DAOQJH010000000
VERSION     DAOQJH010000477.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2410)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2410)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2410
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32368_length_2410_cov_4.534183"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..691
                     /locus_tag="PLQ18_10565"
     CDS             <1..691
                     /locus_tag="PLQ18_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015821894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNE02140.1"
                     /translation="IGAAYGSAGERCMAISVAVAVGEATADALVARLAPRVAGLRVGP
                     GDDALAEMGPLVSAEHRARVAGYIARGIEEGAEAVVDGRGLRIEGCEDGFFLGGTLFD
                     RVRPDMAIWREEIFGPVLAIVRVPDYAAALALVNSHEYGNGAALFTRDGDTARHFTEH
                     VSAGMVGINVPIPVPMAFHSFGGWKASLFGDHHVHGPEGVRFYTRLKTVTSRWPTGIR
                     AGAQFAMPTLG"
     gene            940..2097
                     /locus_tag="PLQ18_10570"
     CDS             940..2097
                     /locus_tag="PLQ18_10570"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNE02141.1"
                     /translation="MSAVRSIADHLFAPLRVQSTDIADLFGVWNHSSQLRRQRRVVLK
                     SRTRLMAALFALLVPLWILPDLLLLDTDHWLPLAGLRVASAVVFMGLAWSRDPGCGRL
                     EALLRVGLLLAVPTVFHLLAAPLLADLDTGGPRGVLLQVYELLPFVVVAGLAVFPLTV
                     LETLLYALPIFMAYLYGVHINGEFRFAENVGTLWLLLLVTGAAMVSSLSQLQYMIALV
                     RRASADTLTGALNRAAGEETLELLFGMAVARGEPLALMFIDLDRFKSINDNWGHEAGD
                     TVLRAMAGQVRTALRRGDVLVRWGGEEFLVLLANSDVHGARLVVERLRAAGFGERPDG
                     APLTASIGLAERLIDRVSDWPELIEIADQRMYAAKLGGRDRAILPGETIVR"
     gene            2194..>2410
                     /locus_tag="PLQ18_10575"
     CDS             2194..>2410
                     /locus_tag="PLQ18_10575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE02142.1"
                     /translation="MAVPLKLLVIEDVETDFLLAVRVLTRHGYAPQATRVDRLDALEA
                     ALAEGGWELAISDYNLPALSFDASFARV"
BASE COUNT          309 a          837 c          871 g          393 t
ORIGIN      
        1 gatcggcgcc gcctacggct cggccggcga gcgctgcatg gcgatctcgg tggcggtcgc
       61 ggtcggcgag gccaccgccg atgcgctggt cgcacggctg gcaccgcgcg tcgccggcct
      121 gcgcgtcggt cccggggacg atgcgctggc cgagatggga ccgctggtca gcgccgaaca
      181 ccgcgcgcgc gtcgccggct acatcgcccg cggcatcgag gagggcgccg aggcggtcgt
      241 cgatggccgc ggcctgcgca tcgagggctg cgaggacggc ttcttcctcg gcggcacgct
      301 gttcgaccgc gtgcgcccgg acatggcgat ctggcgcgag gagatcttcg gcccggtgct
      361 cgcgatcgtg cgcgtgccgg actacgcggc cgcactggcg ctggtcaaca gccacgagta
      421 cggcaacggc gcggcgctgt tcacgcgcga tggcgatacc gcgcgccact tcaccgagca
      481 cgtcagcgcc ggcatggtcg gcatcaacgt gccgatcccg gtgccgatgg ccttccacag
      541 cttcggcggc tggaaggcga gcctgttcgg tgaccaccac gtgcacggtc ccgagggcgt
      601 gcgcttctat acccggctga agacggtcac cagccgctgg ccgaccggca tccgcgccgg
      661 tgcgcagttc gccatgccga cgcttggctg aaccatcccg cacacaattg cgacgctgct
      721 tgcgtcgggt tcgcatttgc gaagccggca cgaatggccg gaaacattga tcctgagcaa
      781 tgcaggcgac ggtgtgctcc cgcatcgtgc atgaccgtcg catcacgggg ccgtgatgcg
      841 caatggccgt ggctgcggct tgcgaatcac cgtgccccgg accggtcagg aagccggtga
      901 tccgcggggc gcaccaccga ccccccgagg cccctgccga tgtctgcggt ccgctcgatc
      961 gccgatcacc tgtttgcgcc gctgcgcgtg cagtcgaccg acatcgccga cctgttcggc
     1021 gtctggaacc attcctcgca gctgcgccgc cagcggcgcg tggtgctgaa gtcgcgcacg
     1081 cggctgatgg ccgcgctgtt cgcgctgctg gtgccgctgt ggatcctgcc cgacctgctg
     1141 ctgctcgaca ccgaccactg gctgccgctc gccggcctgc gcgtggcgag tgcggtggtg
     1201 ttcatggggc tcgcgtggtc gcgggatccg ggctgcgggc ggctggaggc gctgctgcgc
     1261 gtcggcctgc tgctggcggt gccgaccgtg ttccacctgc tcgcggcgcc gctgctcgcc
     1321 gatctcgata ccggcggccc gcgcggcgtg ctgctgcagg tctacgagct gctgcccttc
     1381 gtcgtcgtcg ccgggctggc ggtgtttccg ctgacggtgc tggagacgct gctgtatgcg
     1441 ctgccgatct tcatggccta tctgtacggc gtgcacatca acggcgaatt ccgcttcgcc
     1501 gagaacgtcg gcacgctgtg gctgctgctg ctcgtgaccg gcgcggcgat ggtgtcgagc
     1561 ctgagccagc tgcagtacat gatcgcgctg gtgcgccggg ccagcgccga cacgctgacc
     1621 ggggcgctga accgcgccgc cggcgaggaa acgctcgaac tgctgttcgg catggcggtc
     1681 gcgcgcggcg agccgctggc gctgatgttc atcgatctcg accgcttcaa gagcatcaac
     1741 gacaactggg gtcacgaggc cggcgacacg gtgctgcgcg cgatggccgg gcaggtgcgc
     1801 acggcgctgc gccgcggcga cgtgctggtg cgctggggcg gcgaggaatt cctcgtgctg
     1861 ctcgccaaca gcgacgtgca cggggcgcgg ctggtggtcg agcggctgcg cgcggccggc
     1921 ttcggcgagc ggcccgacgg cgcgccgctg acggcgagca tcgggctcgc cgagcgcctg
     1981 atcgaccgcg tcagtgactg gccggagctg atcgagatcg ccgaccagcg catgtacgcg
     2041 gccaagctcg gcgggcgtga ccgggcgatc ctgcccggcg agaccatcgt ccgctaagct
     2101 gctccggtcg ttgcctcaaa gcccactcaa gggctagggt tatgcggctc cccgccggga
     2161 cgcaagcctt cgaggcccac gggagcccac gtcttggccg taccgctgaa gctgctggtc
     2221 atcgaggatg tcgaaaccga cttcctgctc gccgtgcgcg tgctgacgcg ccacggctat
     2281 gcgccgcagg ccacgcgcgt cgatcgcctc gatgccctcg aagccgcgct cgccgagggc
     2341 ggctgggagc tggcgatctc ggactacaac ctgccggccc tgagcttcga tgccagtttc
     2401 gcgcgcgtgt
//
LOCUS       DAOQJH010000478         2402 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_32583_length_2402_cov_3.944610, whole genome shotgun sequence.
ACCESSION   DAOQJH010000478 DAOQJH010000000
VERSION     DAOQJH010000478.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2402)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2402)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2402
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32583_length_2402_cov_3.944610"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..336)
                     /gene="arfB"
                     /locus_tag="PLQ18_10580"
     CDS             complement(<1..336)
                     /gene="arfB"
                     /locus_tag="PLQ18_10580"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012701998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HNE02143.1"
                     /translation="MHIPDLPEAELDFRPIRAQGPGGQNVNKVETAVQLRFDIGASSL
                     PEAVKARLLRLRDARIHAGGIIVIKAQRHRSQADNRADALARLQALIRSVWTAPKPRR
                     PTQPSRAARE"
     gene            complement(352..867)
                     /locus_tag="PLQ18_10585"
     CDS             complement(352..867)
                     /locus_tag="PLQ18_10585"
                     /inference="COORDINATES: protein motif:HMM:NF022214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 family protein"
                     /protein_id="HNE02144.1"
                     /translation="MPSPRDSLPAVTPAFDPETPAGIGGWLLLMLLGLVLTPLRVAWS
                     TYGMLQPVLMPGVWEQLTEPGQPVYHPLWAPLIVAETAGNGLTLLLALLALWLFLRRS
                     RRAPAVIIGLFVWSLLFAITDQAATGLIPMIPPPTAETLVDALVAPAVAVAIWVPYLL
                     RSKRVRATFVL"
     gene            complement(900..1991)
                     /locus_tag="PLQ18_10590"
     CDS             complement(900..1991)
                     /locus_tag="PLQ18_10590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010094048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HNE02145.1"
                     /translation="MNALLPRLRTLAAALATLALLAHAATGTAEPLRRYNVDPASVSV
                     SGISSGGYMAVQFAVAYSGIVHGVGVVAGGPYGCANASITTALDVCMAGKRPIRVADL
                     VSLTKRRARSGAIDPVAGLAAQRVWIFTGSIDSTVVPKVGDALEQYYRAFVPAASITR
                     VSSVRAEHTMPTTDFGNDCTIKGDPYISDCDFDGAGELLQWIHGPLQPPAATAPGGRL
                     TPFEQNEFSSFPAAHSLGSTGWVYVPQACEDGARRCRLHVAFHGCRQFADFLYVDANN
                     QPARYGTTFAEHAGYNRWADANDFIVLYPQTRRGPELPGDLFGVTSANPKGCWDWWAY
                     DSTQYAEKNGPQMLAVRRMIARISGEQPH"
     gene            complement(2073..>2402)
                     /locus_tag="PLQ18_10595"
     CDS             complement(2073..>2402)
                     /locus_tag="PLQ18_10595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02146.1"
                     /translation="ACQRLQRFAAEQRLALPGNTVAAAGLPAAAVSRRIEVSAAPAVA
                     VHDCAAWGPGSIAYRDCRQRERARLDAWCRQLNRDADRLGGEARQTALDWREAVCSAA
                     ERYTVMR"
BASE COUNT          354 a          813 c          905 g          330 t
ORIGIN      
        1 ctcacgggcg gcgcggctcg gctgcgtcgg ccggcgcggt ttcggtgccg tccagaccga
       61 gcggatcagc gcctgcaggc gcgcgagcgc atcggcacgg ttatcggcct gcgaacggtg
      121 gcgctgggcc ttgatgacga tgatgccgcc ggcgtggatg cgcgcatcgc gcaggcgcag
      181 cagccgcgcc ttgaccgcct cgggcagcga cgaggcgccg atgtcgaagc gcagctgcac
      241 ggcggtctcg accttgttga cgttctggcc gcccggcccc tgcgcgcgga tcggccggaa
      301 gtcgagttct gcctcgggca ggtcagggat gtgcatgccg gacaccggac ttcagagcac
      361 gaaggtcgcg cgcacgcgct tcgagcgcag caggtacggc acccagatcg cgaccgcgac
      421 cgccggtgcg accagcgcat cgaccagcgt ttccgcggtg ggcggcggaa tcatcgggat
      481 cagcccggtg gccgcctggt cggtgatcgc gaacagcagc gaccagacga acaggccgat
      541 gatcacggcc ggtgcccgcc gcgagcggcg caggaacagc cacagcgcca gcagcgcgag
      601 cagcagcgtc aggccgttgc cggcggtttc ggcgacgatc agcggtgccc acagcgggtg
      661 atagaccggt tggccgggct cggtcagctg ctcccagacg ccgggcatca gcaccggctg
      721 cagcatcccg tacgtcgacc aggccacgcg cagcggcgtc agcaccagcc cgagcagcat
      781 cagcagcagc cagccgccga tgccggccgg ggtttcgggg tcgaaggccg gcgtgacggc
      841 cggcagggaa tcgcgggggc tcggcatcgg ggtgtccgga ggtctgggca gggcgggctt
      901 cagtgtggct gctcgccgct gatgcgcgcg atcatccggc gcacggcgag catctgcgga
      961 ccgttcttct cggcgtactg cgtgctgtcg taggcccacc agtcccagca gcccttcgga
     1021 ttggccgacg tgaccccgaa caggtcgccc ggcagctccg ggccgcgccg ggtctgcgga
     1081 tacagcacga tgaagtcatt ggcatcggcc cagcggttgt agccggcgtg ctcggcgaag
     1141 gtcgtgccgt agcgggccgg ctgattattc gcatccacgt acaggaagtc cgcgaactgg
     1201 cggcagccgt ggaaggcgac gtgcagccgg cagcggcgcg ccccgtcctc gcaggcctgc
     1261 ggcacgtaga cccagccggt gctgccgagg ctgtgtgcgg ccgggaagct gctgaactcg
     1321 ttctgttcaa agggcgtcag gcggccgccc ggtgcggtgg ctgccggcgg ctgcagcggc
     1381 ccgtggatcc actgcagcag ctcgccggcg ccgtcgaaat cgcagtcgct gatgtacggg
     1441 tcgcccttga tcgtgcagtc gttgccgaag tcggtggtcg gcatcgtgtg ctcggcgcgc
     1501 acgctgctga cgcgcgtgat cgaggcggcc ggcacgaagg cccggtaata ctgttccagc
     1561 gcatcgccaa ccttcggcac gacggtcgag tcgatgctgc cggtgaagat ccacacgcgc
     1621 tgcgcggcca ggcccgcgac cggatcgatc gcgccgctgc gcgcccggcg cttggtcagc
     1681 gacaccagat cggcgacgcg gatcggacgc ttgccggcca tgcacacgtc gagcgcggtc
     1741 gtgatgctgg cgttggcgca accatacggg ccgccggcga cgacgccgac cccgtgcacg
     1801 atccccgaat aggcgaccgc gaactgcacg gccatgtagc cgccggagga aatccccgat
     1861 accgacacac tggccgggtc gacgttgtag cggcgcagcg gttcggccgt gccggtggcg
     1921 gcgtgcgcga gcagggccag cgtggcgagt gcagcagcga gcgtgcgcag gcggggcagg
     1981 agagcattca tgccggggtc ctcattcggc ggcgacaggg gcgggcatcg ccgcaccggg
     2041 gggcgcgcgg cgcacgtcag gggatttcgg gttcagcgca tgaccgtata ccgctctgcg
     2101 gccgagcaga cggcttcgcg ccagtcgagt gccgtctgcc gggcctcgcc gccgaggcga
     2161 tcggcatcgc ggttgagttg ccggcaccag gcatcgagcc gggcacgctc ccgctggcgg
     2221 cagtcgcgat acgcgatcga gccgggaccc caggccgcgc agtcatggac ggcaacggcc
     2281 ggggcggcgc tcacctcgat ccggcgtgac acggcagccg ccggcagccc tgcggccgcg
     2341 acggtattgc cgggcagggc cagtcgctgt tcggcggcga agcgctgcag ccgctggcag
     2401 gc
//
LOCUS       DAOQJH010000479         2389 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_32894_length_2389_cov_3.947729, whole genome shotgun sequence.
ACCESSION   DAOQJH010000479 DAOQJH010000000
VERSION     DAOQJH010000479.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2389)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2389)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2389
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32894_length_2389_cov_3.947729"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="PLQ18_10600"
     CDS             <1..200
                     /locus_tag="PLQ18_10600"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HNE02147.1"
                     /translation="LPRVRERFYRGSAGLASHGAGLGLYLVEQFVQLSGGRLDIDSPP
                     GQGTVVRLVLPVHGPRAAAAA"
     gene            218..1906
                     /locus_tag="PLQ18_10605"
     CDS             218..1906
                     /locus_tag="PLQ18_10605"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNE02148.1"
                     /translation="MDTNGAVILCVEDEAEIRRDLVDELLDAGYEVHEAGSGSEAIGR
                     LAGLQPDLILCDVNMPGLSGLQLLEQVRARGDALGEVPFVLLTAYGDREHQIRGRALG
                     ADDYLIKPVDYDLLLATLASRLRNARRVHARVQSRIGDLESQLAGVERRTATLPDRMA
                     LQAALADGRGRRRRGLLVASFDELSRHVQQFGSSFQDEAEQALIGQVQLLAPAQAYRL
                     DSETFGLLFADADALDVSALGRLVEIECRSPGGAAPVRLSTTLCYVHCEPEAGCDVAD
                     RLEDACLAVRFGRRDGGHTLLRLDAAAVQRLRMAGHVEDHLLRALRDGEFTLHFQPKL
                     QLADDRLVGAEALVRWHSRDLGPISPGVFIPIAERCNLIEALSVWVLDEAARAAAVLA
                     REGLDLAIAVNASGVELHAGFPARVREVMAAHGLGAGQLDIELTETSVIADIRHAAGI
                     VAELRGLGVGVAIDDFGTGYGSLSYLRALPVDTVKIDQSFVRGLPGNAIDRQIIESVL
                     TLAHALGIGTVAEGVETAEQEREMRRLGCGQIQGYHLARPMPLTELIRFVRERR"
     gene            1935..2315
                     /locus_tag="PLQ18_10610"
     CDS             1935..2315
                     /locus_tag="PLQ18_10610"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE02149.1"
                     /translation="MTMTPAGTTLLVVEDEQELREYLVEELEDLGYQVCEAGNGALAL
                     ALLRERPVDLVLTDIMMPQLGGAGLYHATRCDPALAQLPFLFLTALSSAEMLATLPES
                     EQPHILKKPVDYDELADAIRARLA"
BASE COUNT          332 a          835 c          872 g          350 t
ORIGIN      
        1 agctgccgcg cgtgcgcgag cgtttctacc gcggcagtgc cgggcttgcg agtcacgggg
       61 ccgggctcgg cctgtatctg gtcgagcagt tcgtgcagct gagcggcggg cggctggaca
      121 tcgacagccc gccggggcag ggcacggtcg tgcgtctggt gctgccggtg catggtccgc
      181 gcgccgcggc agcggcctga acggaggagg gcccgtcatg gacacgaacg gtgcagtgat
      241 cctgtgcgtc gaggatgaag ccgaaatccg ccgcgatctc gtcgacgaac tgctcgatgc
      301 cggctacgag gtgcacgagg ccggcagcgg cagcgaggcg atcgggcggc tcgccggcct
      361 gcagcccgac ctgatcctct gcgatgtcaa catgcccggc ctgagcggcc tgcagctgct
      421 cgaacaggtg cgtgcgcgcg gggatgcgct cggcgaggtg ccgttcgtgc tgctgaccgc
      481 ctacggcgac cgcgaacacc agatccgcgg ccgcgcgctc ggggccgatg actacctgat
      541 caagccggtc gactacgacc tgctgctcgc cacgctcgcg agccgcctgc gcaacgcccg
      601 gcgcgtgcat gcgcgcgtgc agtcgcgcat cggcgatctc gaatcgcagc tggccggggt
      661 ggagcgcagg acggccacgc tgccggaccg gatggccttg caggcagccc ttgccgacgg
      721 gcgcggccgg cgccggcgcg gcctgctggt tgcgtccttc gatgagctca gccgccacgt
      781 ccagcaattc ggcagcagct tccaggacga ggccgagcag gcgctgatcg ggcaggtgca
      841 gctgctggct ccggcgcagg cctaccgcct cgattccgag accttcggcc tgctgttcgc
      901 cgatgccgat gcgctcgatg tgtcggcact cgggcggctg gtcgagatcg aatgccgcag
      961 cccggggggc gccgcgccgg tgcgcctgag cacgacgctc tgctacgtgc actgcgagcc
     1021 cgaggccggc tgcgacgtgg ccgaccggct ggaggatgcg tgcctcgccg tgcgcttcgg
     1081 ccggcgcgac ggcggccata cgctgctgcg cctcgacgcg gcggccgtgc agcggctgcg
     1141 catggccggc cacgtcgagg atcacctgct gcgggcgctg cgcgacggcg agttcacgct
     1201 gcacttccag cccaagctgc agctcgccga tgatcgtctg gtcggtgccg aggcgctggt
     1261 gcgctggcac agccgggatc tcggaccgat ctcgcccggc gtgttcatcc cgatcgccga
     1321 gcgctgcaac ctgatcgagg cgctgagcgt ctgggtgctc gacgaggccg cgcgcgccgc
     1381 ggcggtgctc gcgcgcgagg ggctggatct tgcgatcgcc gtcaatgcct cgggtgtcga
     1441 actgcacgcc ggcttcccgg cgcgcgtgcg cgaggtcatg gccgcgcatg gtctcggtgc
     1501 cggccagctc gacatcgagc tgaccgaaac ctcggtgatc gccgacatcc gccacgccgc
     1561 cggcatcgtc gccgagctgc gcgggctcgg cgtcggcgtc gcgatcgacg atttcggcac
     1621 cggctacggc tcgctttcgt acctgcgcgc gctgccggtc gataccgtca agatcgacca
     1681 gagcttcgtg cgcggcctgc ccggcaacgc catcgaccgt cagatcatcg agagcgtgct
     1741 gacgctcgcg cacgcgctcg gcatcggcac ggtcgccgaa ggcgtcgaga ccgccgagca
     1801 ggagcgcgag atgcgccgcc tcggctgcgg ccagatccag ggctatcacc tggcgcggcc
     1861 gatgccgctc accgaactga tccgcttcgt gcgcgagcgg cgctgagccc gccacagaca
     1921 agaaacggga cgggatgacg atgacaccgg ccggcacgac gctgctcgtg gtcgaggacg
     1981 agcaggagct gcgcgagtac ctggtcgagg aactcgaaga cctcggctat caggtctgcg
     2041 aggccggcaa cggcgcgctg gccctggcgc tgctgcgcga gcggccggtc gatctggtgc
     2101 tgaccgacat catgatgccg cagctcggcg gtgccgggct ctaccacgcc acgcgctgcg
     2161 atccggcact cgcgcagctg ccgttcctgt tcctgaccgc gctgtcctcg gccgagatgc
     2221 tggcgacgct gccggagagc gagcagccgc acatcctgaa gaagccggtc gactacgacg
     2281 agcttgccga cgcgatccgg gcgcggctgg cgtagccaga cggacgccgc tagccggcgt
     2341 gcgggcgttc gagcacgacc gcgaccccca tgccgccggc cgtgcagac
//
LOCUS       DAOQJH010000480         2377 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_33237_length_2377_cov_4.322136, whole genome shotgun sequence.
ACCESSION   DAOQJH010000480 DAOQJH010000000
VERSION     DAOQJH010000480.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2377
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33237_length_2377_cov_4.322136"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..324)
                     /locus_tag="PLQ18_10615"
     CDS             complement(<1..324)
                     /locus_tag="PLQ18_10615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019465684.1"
                     /note="This protein performs the mismatch recognition step
                     during the DNA repair process; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HNE02150.1"
                     /translation="MMQQYLRTRAEHPGVLLFYRMGDFYELFYDDARKAARLLDITLT
                     ARGASAGEPIPMAGVPYHALEGYLAKLVRLGESVAICEQIGDPATSKGPVERKVVRVV
                     TPGTLT"
     gene            1051..>2377
                     /gene="glgC"
                     /locus_tag="PLQ18_10620"
     CDS             1051..>2377
                     /gene="glgC"
                     /locus_tag="PLQ18_10620"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HNE02151.1"
                     /translation="MHPTKPVMPNVESRFVSRLTRETLALVMAGGRGTRLRNLTQHRA
                     KPAVPFGGKFRIIDFTLSNCINSGIRRIGVLTQYKSHALIRHLERGWSFLRGQFGEFI
                     ELLPAEQNSDLSSWYVGTADAVYQNIEFIRRHQPRYVLVLAGDHVYNMDYGPMLAHHA
                     ASGADITIGCIEVPLSEAVDFGVMEIDESTRVHGFIEKSPNPKPMPGRPDTALASMGI
                     YVFEARYLLDVLKRDAGMRNSGHDFGHDVIPEAIRAGRVHAFPFRDLFDPDRQGYWRD
                     VGTVDAFWRANIELCGAEPVLNLDDERWPVWTGEEQSAPARFSSVPDGPRSVIEDALC
                     CGGCTVTGATVERSVLFTGARVEAGSRIEESLLLPDVRVGRNCRIRRAVIDEGCVVPD
                     GLVIGEDAASDQRLFHRSSEGIVLVTQEMLDNLQARGAGAAMPLPAVPPE"
BASE COUNT          384 a          793 c          823 g          377 t
ORIGIN      
        1 ggtcagcgtg ccgggcgtga ccacgcgcac caccttgcgc tcgaccggcc ccttgctggt
       61 cgccggatcg ccgatctgct cgcagatcgc caccgactcg ccgaggcgca cgagcttcgc
      121 gagatagcct tcgagcgcgt ggtagggcac gccggccatc gggatcggct cgccggccga
      181 ggcgccgcgc gcggtcagcg tgatgtcgag caggcgtgcg gccttgcggg cgtcgtcgta
      241 gaacagctcg tagaaatcgc ccatccggta gaacagcagc accccggggt gctcggcccg
      301 ggtgcgcagg tactgctgca tcatcggcgt gtggcgggcg tacggatcgg cggcagcgac
      361 ggtcacggca ggtctggggc tcgggcggac gcgggaccgc gcatggtgag acgcgcgcgc
      421 cacggcagca agcacggtgc gggctttgcg cgatgtgcaa gtgcagcgac agagcggatc
      481 gcgcggcgca gcaggctgaa cgtttggcaa tgaaagtgaa ctgtcgttgc gggaacgctt
      541 tgcccttgcg ctcatcgaaa gtgctgttcg tcgcttccgg cgtacgggcc gtgattgccg
      601 gaccggcacg gcccgccacc gcaaacgcac ggcgtgcagc gtccccaccg atgcagggcc
      661 acgggccgaa atgtccgggc gagcgtcgct gcgcgcccgc ggcaggcgaa cgggccgcag
      721 gcgcattggc agcgggacgg gcgggattgc cgatggccgc gtcaggcgca caccgcggtt
      781 caggtaatga ttccggtggt tccggcgtaa tggtttcacc cgatggtttc acccgcagtt
      841 tcagcccatt caaggcagta ccggatcaag ggtcggcaga cggaacgggc cggcatgcgg
      901 gtggcccgca atcagcaggg aaaccggcag gcgtctgatt cccgatgctg ccggtccgga
      961 acttccggtg cgggctgcca ttccagatgc gatgacaatg ggccgtgcgc cagccggcga
     1021 acggcggttc gatccgagga gattcagccg atgcacccga ccaagcccgt gatgccgaac
     1081 gtcgagtccc gtttcgtcag tcgtctgacc cgtgaaaccc tggcgctggt catggccggc
     1141 gggcgcggca cgcgcctgcg caacctgacc cagcaccgcg ccaagccggc cgtgcccttc
     1201 ggcggcaagt tccggatcat cgacttcacg ctgtcgaact gcatcaacag cggcatccgc
     1261 cgcatcggcg tgctgacgca gtacaagagt cacgcgctga tccgccatct ggagcgcggc
     1321 tggagcttcc tgcgcggcca gttcggcgaa ttcatcgaac tgctgccggc cgaacagaac
     1381 tccgatctca gttcctggta tgtcggcacc gccgacgccg tttaccagaa catcgaattc
     1441 atccgccgcc accagccgcg ttacgtgctg gtgctggccg gggatcacgt ttacaacatg
     1501 gattacgggc cgatgctcgc gcatcacgcg gccagcggtg ccgacatcac gatcggctgc
     1561 atcgaggtgc cgctgtccga ggccgtcgat ttcggggtca tggaaatcga cgaaagcacg
     1621 cgcgtacacg gcttcatcga aaaatccccg aatccgaaac ccatgcccgg ccgtccggac
     1681 accgcgctgg cgagcatggg catttacgtg ttcgaagcgc gttatctgct cgatgtattg
     1741 aagcgcgacg ccggcatgcg taattccggg catgatttcg gccatgacgt gattccggaa
     1801 gcgatccgcg ccgggcgcgt ccacgccttc ccgttccgcg acctgttcga tcccgacagg
     1861 cagggctact ggcgcgatgt cggtaccgtc gatgccttct ggcgcgccaa catcgagctc
     1921 tgcggcgccg agccggtgct gaacctcgat gacgagcgct ggccggtgtg gaccggcgag
     1981 gaacagtcgg cgccggcgcg cttctcgtcg gtgccggacg gcccgcgcag cgtgatcgag
     2041 gatgcgctct gctgcggcgg ctgcacggtg accggcgcca cggtcgagcg ctcggtgctg
     2101 ttcaccggcg cccgcgtcga ggccggcagc cgcatcgagg aatccctgct gctgcccgac
     2161 gtgcgcgtcg ggcgcaactg ccgcatccgc cgcgccgtca tcgacgaggg ctgcgtcgtg
     2221 cccgacggcc tcgtgatcgg cgaggacgcc gccagcgacc agcgcctgtt ccaccgcagc
     2281 agcgaaggca tcgtgctggt cacgcaggag atgctcgaca acctgcaggc gcgcggggcc
     2341 ggggccgcca tgccgctgcc ggccgtgccg ccggaga
//
LOCUS       DAOQJH010000481         2376 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_33271_length_2376_cov_3.457131, whole genome shotgun sequence.
ACCESSION   DAOQJH010000481 DAOQJH010000000
VERSION     DAOQJH010000481.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2376
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33271_length_2376_cov_3.457131"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..691)
                     /locus_tag="PLQ18_10625"
     CDS             complement(<1..691)
                     /locus_tag="PLQ18_10625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02152.1"
                     /translation="MPDTDWKQRYFDVLAEHEQAEAAWKAREEMLGFAVNRLSLLGQG
                     LDARLDPHLESLRAGLRRGDSTERLRAEAGRLADLLDELRRLGHAGRDDELLHLLELL
                     RLPPSRRKALDPLAVRVRQTQPPRGELIEAIAAELNHILLDPSRAPLAAGLPAPGSLA
                     GEDLDGAAPEAEPERSGRTLTDLLAALRLPWTPELELARETLRQRLAQPVPAAAWPQA
                     IEAGAELLNRAL"
     gene            1106..2104
                     /locus_tag="PLQ18_10630"
     CDS             1106..2104
                     /locus_tag="PLQ18_10630"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNE02153.1"
                     /translation="MTPAISVILPVRDGGDWLREAVDSVRAQSFGDFELLLIDDGSTD
                     DAIARLPPDARLRVLANPGRGLVAALNHGMAQAQGAFLARMDADDRCRPQRFERQLAL
                     LAARPEVAICGVQVELLQPDGGPPPPGGLAYRQWLNALCEPADIARELFIEAPLPHPG
                     WLIRRTAWTRVGPYRDGPWPEDYDWLLRAHLAGLTMAKPADGVLLDWREHPQRLTRRD
                     PRCARANLIAVKAPALAVVLGGREVLLWGATKTGGRLHDALTAQGVRIRAFVDLDPRK
                     RAGRKRDLPVLAPEAAAAAADAAILVTVSQGAKKPAIRAPRGALGRREGIDWWFAT"
BASE COUNT          298 a          893 c          874 g          311 t
ORIGIN      
        1 ccagcgcccg gttcagcagc tcggcgccgg cctcgatcgc ctgcggccag gccgcggccg
       61 gcaccggctg cgccaggcgc tggcgcaggg tctcgcgcgc gagttcgagt tccggcgtcc
      121 acggcaggcg cagcgccgcg agcagatcgg tcagcgtgcg gccgctgcgt tccggctcgg
      181 cttccggcgc cgcaccgtcg aggtcctcgc ccgcgaggct gccgggtgcc ggcaggccgg
      241 cggcgagcgg ggcgcgcgag ggatcgagca ggatgtggtt cagctcggcg gcgatcgcct
      301 cgatcagttc gccgcgcggc ggctgcgtct ggcgcacgcg cacggccagc ggatcgagcg
      361 ccttgcgccg cgacggcggc aggcgcagca gttcgagcag gtgcagcagt tcgtcatcgc
      421 gaccggcatg accgaggcgg cgcagctcgt cgagcagatc ggcgaggcgc ccggcctcgg
      481 cgcgcagccg ctcggtgctg tcgccgcggc gcaggccggc acgcaggctt tcgaggtgcg
      541 gatcgagccg cgcatccagc ccctgcccga gcagcgacag ccggttgacc gcgaagccga
      601 gcatctcctc acgcgccttc cacgccgcct cggcctgctc gtgctcggcg agcacatcga
      661 aataacgctg cttccagtcg gtgtccggca ttgcgaaact ccgggcgaaa cccagcgggg
      721 ctggcgatcg gacgggcgga acctgcgtgc ggacgagcct gcgcagcgcg ccggcccggc
      781 cgtttctccc cggtccgccg gggggaaggg caatgcatga catcggaacg tgaccggcac
      841 ggcccgccat tgcggccggg gcgccccggc ggccaggcgg ttctgccctt gcggagtccg
      901 cgatccccga cgcaggttcc ggcgaggaca cttgcgggcc gccgatgctg cgccggtccg
      961 cgacggccag caagcggcaa gcgcagcaga cttccggagt gctcttgcaa gaccctgccc
     1021 ccctcgcctt tcccgccccg aaccgtgcag ccgcccgtgg attgcgccgc cccgcgcgcc
     1081 gcattcaccc ctcgctcagc cgccgatgac gcctgcgatt tcggtgatcc tgccggtgcg
     1141 cgacggcggc gactggctgc gcgaggccgt cgacagcgtg cgagcacaga gcttcggcga
     1201 cttcgagctg ctgctgatcg acgacggctc gaccgacgac gccatcgcgc gcctgccgcc
     1261 ggatgcgcgc ctgcgcgtgc tcgccaaccc cggccgtggg ctggtcgcgg cgctcaacca
     1321 cggcatggcg caggcgcaag gcgcgtttct ggcgcgcatg gacgccgacg accgctgccg
     1381 gccgcagcgc ttcgagcgcc agctcgcgct gctggcggcg cgcccggagg tggcgatctg
     1441 cggcgtgcag gtcgagctgc tgcagcccga cggcggtccg ccgcctccgg gcggcctcgc
     1501 gtaccggcaa tggctgaacg cgctctgcga gccggccgac atcgcccgcg agctgttcat
     1561 cgaagcgccg ctgccgcatc ccggctggct gatccggcgc acggcctgga cgcgggtcgg
     1621 gccgtatcgc gacggcccgt ggccggagga ttacgactgg ctgctgcgcg cccacctggc
     1681 cggcctgacg atggccaagc ccgccgatgg cgtgctgctc gactggcgcg agcacccgca
     1741 gcgcctgacc cgccgtgacc cgcgctgcgc ccgcgcgaac ctgatcgcgg tgaaggcccc
     1801 ggcgctcgcc gtcgtgctcg gcgggcgcga ggtgctgctg tggggcgcga cgaagaccgg
     1861 cggccgcctg cacgacgcgc tcacggcgca gggcgtgcgc atccgcgcct tcgtcgatct
     1921 cgacccgcgc aagcgcgccg ggcgcaagcg cgacctgccg gtgctggccc ccgaggccgc
     1981 cgccgccgcc gccgacgccg cgatcctcgt aaccgtctcg cagggcgcga agaagccggc
     2041 gatccgcgcc ccgcgcggcg cgctcggccg gcgcgagggc attgactggt ggtttgcgac
     2101 ctgagcggcc ggcacactcc gcgcccggtc ttccgtgccg accgcccttt tccccgccag
     2161 ccgctccgaa ccccgcatga acccgcaggc cctgtcgtcg tgcatccttg caggcggcgt
     2221 cgcgaacagc gtgcgcatgt ccggaggact ggccgaatga gcgaactgca gatctatcgg
     2281 aacgccgcag gcgcggtgga agtacggctc gatggcaaca gcgtctggct gcggcaggag
     2341 cagatggcgg tgctgttcgg gcgggatcgc acggtg
//
LOCUS       DAOQJH010000482         2376 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_33280_length_2376_cov_2.580353, whole genome shotgun sequence.
ACCESSION   DAOQJH010000482 DAOQJH010000000
VERSION     DAOQJH010000482.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2376
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33280_length_2376_cov_2.580353"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..250)
                     /locus_tag="PLQ18_10635"
                     /pseudo
     CDS             complement(1..250)
                     /locus_tag="PLQ18_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011086305.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
     gene            564..2222
                     /locus_tag="PLQ18_10640"
     CDS             564..2222
                     /locus_tag="PLQ18_10640"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HNE02154.1"
                     /translation="MRSSIRRLAVLCLSLIAGAALAAPQVRDARVAEARDRVQLILDL
                     SEPARYTTYFVDQPPQVVIDLPDAEPSDALKDRSYFGKSKAIRSIRGGKRQDGGIRLI
                     VRLDQPARPSTWLIPAADGKPDRLVIEMPAGKAQARGERLPDIDFESSPRTAGRPAER
                     APQRLARAVRRDIVIAIDPGHGGKDTGAIGLRDVREKDVVLAIGRRLATLIDREPGMR
                     AVMTRRGDTFLPLRSRMSVARKQKADLFISIHADSAPNGSLASGSSVYVLSTRGASSE
                     AARWLADRENASDMAGGLRLAAAHDREIASVLLDMSQGASMESSLDLGKRVLNQLDHI
                     GPLHSNSVERAAFAVLKSPDIPSILIETAYLSNPAEESRLASDEGQRQLAQAIMDGIR
                     GYYRNRLPHSVMVADTDNAPRSRAGADRDDDGDAAPAARPAVARAVSKPEARKDTLPK
                     LTLTSSGQESRAGKAKVKPDAGAKPASHNTPAPAARPASARPAVAQSSASAAARNHVV
                     QRGESLQDIARQYRLPVTRLLAMNRLPSNQLRVPAGTTLVVPGS"
BASE COUNT          399 a          889 c          774 g          314 t
ORIGIN      
        1 tggcagagag cacgagcagc cggcttgcac agccgcgcgc caagatcatg ccgcaaccga
       61 acatggcccc gccgatcagt gcgcccgaaa tgctgccacg ctgcgccagc tgccgcgctt
      121 ctgagacatc gagaagaccg gcgacgatcg cagcctgcgt acacgcaacc gccgccgaaa
      181 acgccagcac ccagatcgcg accttgggcc ccagctcccg gtgcgcgaac tcgatcacgc
      241 cggcacgcag cggcacgcaa cgctcacggc tgcgagcgcg accgggcagg cgctgctgac
      301 ggcgctggag ctgcccgccg cgcaggtctg atgccggtcg cacgcatcgc gcgtgcatgt
      361 cgggtttccc cgtcgtcccg gaccttcctg cacgaagaat gaagctctga agcgcgtgta
      421 ttcctgcgtg acgggcacga aacgcaacga ctatcactgt cgcaacaacc gaatgcagaa
      481 aagccacagc ttgccctgca gatacgtgtc gtgcgtgtca tactcctgcg tatcttttct
      541 gccgcccgta cacgcccacg ccgatgcgct cgtccatccg tcgtctggcc gtcctctgcc
      601 tgtctctcat cgccggtgcc gcccttgcag cgccgcaggt gcgggatgcg cgcgtcgccg
      661 aagcgcgcga tcgcgtgcag ctgatcctcg atctgtcgga accggcgcgc tacacgacgt
      721 atttcgtcga tcagccgccg caggtcgtca tcgacctgcc cgacgccgag ccttccgatg
      781 cgctgaagga tcgcagctac ttcggcaagt cgaaggccat ccgcagcatc cgcggcggca
      841 agcgccagga cggcggcatc cggctgatcg tgcggctcga ccagccggcg cgaccgtcga
      901 cctggctgat cccggcggcc gacggcaagc cggaccggct ggtgatcgag atgccggccg
      961 gcaaggcgca ggcacgcggc gagcggctgc ccgacatcga cttcgaaagc tcgccgcgca
     1021 ccgccggccg gccggccgag cgggcaccgc agcggctggc acgcgccgtg cgccgcgaca
     1081 tcgtcatcgc catcgacccc ggccacggcg gcaaggacac cggcgcgatc ggcctgcgcg
     1141 acgtgcgcga gaaggacgtc gtgctcgcga tcggccggcg gctggcgacg ctgatcgacc
     1201 gcgaaccggg catgcgcgcg gtgatgaccc gccgcggcga caccttcctg cccctgcgct
     1261 cgcgcatgag cgtcgcacgc aagcagaagg ctgatctctt catctcgatc cacgccgact
     1321 cggccccgaa cggctcgctc gccagcggct cgtcggtgta cgtgctgtcg acgcgcggcg
     1381 catcgagcga ggcggcgcgc tggctcgccg atcgcgagaa cgcctccgac atggccggcg
     1441 gcctgcggct ggcggccgcg cacgatcgcg agatcgcctc ggtgctgctc gacatgtcgc
     1501 agggcgcgtc gatggaatcg agcctcgacc tcggcaagcg cgtgctcaac cagctcgatc
     1561 acatcggtcc gctgcactcg aacagcgtcg agcgcgccgc cttcgcggtg ctgaaatccc
     1621 cggacattcc gtcgatcctg atcgagaccg catacctgtc gaatccggcc gaggaatcgc
     1681 ggctcgcgag cgacgaaggc cagcgccagc tcgcgcaggc gatcatggac ggcatccgcg
     1741 gctattaccg caaccgcctg ccgcacagcg tcatggttgc cgataccgac aatgccccgc
     1801 gcagccgcgc cggcgccgat cgggacgacg atggcgatgc cgctccggct gcccggccgg
     1861 cggtcgcccg cgctgtcagc aaacccgaag cccgcaagga cacgctgccg aaactgacgc
     1921 tgaccagctc cggccaggaa tcccgggccg gcaaggccaa ggtcaagccc gatgccggcg
     1981 ccaagcccgc gagccacaac acgccggccc ctgccgcccg cccggccagc gcccggccgg
     2041 cggtcgcgca gagcagcgcc agtgccgcag cgcgcaacca cgtcgtgcag cgcggcgaat
     2101 cgctgcagga catcgcccgc cagtaccggc tgccggtcac gcgcctgctg gcgatgaacc
     2161 gcctgccgtc gaaccagctg cgcgtgccgg ccggcacgac gctggtggtg ccgggcagct
     2221 gagcccgcgg ctccggcgac gatgccgcag ctgtagccgc cggacagaca gcgcctgtct
     2281 gcatctgccg cgctgacgac tgcaccatcg gttcccgaac ttgtccggac agcgcggacg
     2341 gatcagaacg acggcaagac gttgatgaac aagccc
//
LOCUS       DAOQJH010000483         2375 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_33302_length_2375_cov_3.158190, whole genome shotgun sequence.
ACCESSION   DAOQJH010000483 DAOQJH010000000
VERSION     DAOQJH010000483.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2375)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2375)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2375
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33302_length_2375_cov_3.158190"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..795)
                     /locus_tag="PLQ18_10645"
     CDS             complement(<1..795)
                     /locus_tag="PLQ18_10645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02155.1"
                     /translation="MPSTSAASSCSSFWWCQDRAHAVAHQFAAQQVHQRTLARTPVAG
                     DRDGQRRMRVLVAQEVREPARQRPEGQRIGITRLQRLVGGRRIADRRGRCVFGARLAG
                     REQQQAEPEPPDRVQPRRRKACVDRPVAQRRIGVALAAAPGLAAVVGCQQHRPFAGDP
                     ATATVSSEAHAVEQLRARDVGLLPGRAAVEAAQDRATLSDHEQAAAIGRRFHVVHRRS
                     AVGQAGHLLPARTTVRDGPEPSAEVQRPAATAIPCRPHAVQDQSVAA"
     gene            1235..>2375
                     /locus_tag="PLQ18_10650"
     CDS             1235..>2375
                     /locus_tag="PLQ18_10650"
                     /inference="COORDINATES: protein motif:HMM:NF019313.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase fold protein"
                     /protein_id="HNE02156.1"
                     /translation="MRPAAFAQGLAALIAALTALTAAVRAVRAFGVRPEALLASLATK
                     TRVSDLGAQTSFRYRYRQEFAEVTQALAPRTMLILIDDLDRCQPTQVLEMLEVVNFLV
                     SAGDCFIVLGIDKEKVQGALGLAFRELAQEMVDQGSDAADTRSDEARARDKRQRYARD
                     YLEKLINLEVPVPLLDGTDPARGRGMLAGESAAREQAGLRLHRWRERLGSGLRVLAGL
                     FLFIAAAAGGLYLAGLTPALPPPATVGPATTTPAGSPGAAGGDTRPASAGSSAPMPAA
                     QGAASVIGQQAGLPWPLLVAVPALALLLGGLVAALRRPDIVVRDSPRFGEALDIWYPY
                     LLSHHRPTPRTLKRYQNRIRYVAMRQRPAEPATDPLERLLRRWLGV"
BASE COUNT          306 a          835 c          886 g          348 t
ORIGIN      
        1 ggcagcgacg gactggtcct gcaccgcgtg cgggcgacag gggatggccg tcgccgctgg
       61 gcgctggacc tcggcggacg gctctggacc gtcgcggacg gtggtacggg ctggcagaag
      121 gtggccggcc tgcccgacgg cacttcggcg atgtacgacg tggaagcgtc ggccgatggc
      181 cgcagcctgt tcgtggtcgg agagagtggc acggtcctgc gcagcctcga cggcggcacg
      241 acctggcaga agcccgacgt cgcgagcacg cagttgctct acggcgtgcg cttcgctgct
      301 gacggtcgcc gtggctggat cgccggcgaa gggccggtgc tgctgacaac cgacgacggc
      361 ggcgagacct ggcgcggcgg cgagcgcaac accgatccgg cgctgcgcga ctggccggtc
      421 tacgcacgct ttccggcgcc gtggctgtac ccggtctggg ggctcgggct cggcctgctg
      481 ctgctcgcgg cccgcaagcc gcgcgccgaa tacgcaccgg ccgcgtcgat cggcgatgcg
      541 ccggcctccg acaagccgct ggagccgggt gatgccgatg cgctggcctt cgggccgctg
      601 gcgcgcgggc tcgcgaactt cctgcgcaac gagcacacgc atccgccgct gaccgtcgcg
      661 atcaccggca actggggtac gggcaagagt tcgctgatga acctgctgcg cggcgaactg
      721 gtgcgctacg gcgtgtgcgc ggtcttggca ccaccagaag gaagaacagc tgctcgcggc
      781 gctggtcgac ggcatccgcc ggcaggcgat cccgtcgccg ctcggcttcg acggcctgcg
      841 ctttcgcgca cggctgctgt ggctgcgctc tggtcagcac tggccgtggg cgttgctgtt
      901 cgtgctgctc gcatgctggt ccggggccta cttcctgcag gacttcccgc tgcgcttcga
      961 gcgggtctgg gcgatcacgc agcaggtcgt tgccggcatc cgggccgggg agtcgcaggc
     1021 cgatccgccg aagtccggac cggcgaaatc atcggaagac ccctccgggc ttgccgctgc
     1081 gaaggccgcc ggcaacccgc cggactccgg ggccgggacg acctcgaaaa atgcgtctgc
     1141 cggttccggg caggatgcag gtgccgggca gggaagggcg gttgcaggcg ttgcgacagc
     1201 ctctgttgcg cctgctccgg aggcggagcc tgccgtgcgg ccggccgcgt tcgctcaggg
     1261 gctggcggcg ctgatcgctg cgctgacggc gctgacggcg gcggtcaggg ccgtgcgcgc
     1321 cttcggcgtc aggcccgaag ccttgcttgc gagcctcgcg accaagaccc gcgtcagcga
     1381 tctcggcgcg cagacgagct tccgctaccg ctaccggcag gagttcgccg aggtcacgca
     1441 ggcgctcgcg ccgcgcacga tgctgatcct gatcgacgac ctcgatcgct gccagccgac
     1501 gcaggtgctg gagatgctgg aggtcgtcaa cttcctcgtc tcggccggcg actgcttcat
     1561 cgtgctcggc atcgacaagg aaaaggtgca gggcgccctc gggctggcgt tccgggagct
     1621 ggcgcaggag atggtcgatc agggcagcga tgcggcggat acccgcagcg acgaggcccg
     1681 cgcccgcgac aagcgccagc gctacgcgcg cgattacctc gaaaaactca tcaacctcga
     1741 ggtgccggtg ccgctgctcg acggcaccga tccggcgcgt ggccgcggca tgctcgccgg
     1801 cgagtcggct gcgcgggaac aggccggact gcgcctgcac cgctggcgcg aacggctggg
     1861 cagcggcctg cgcgtgctcg ccggcctctt cctgttcatc gcggcggcag ccggcggact
     1921 gtatctggcc ggcctgacac cggcgctgcc gccgccggcg acggtcggcc cggcgacgac
     1981 gacaccggcc ggcagccccg gtgcggccgg cggcgatacc cgcccggcct cggccggatc
     2041 gtctgcgccg atgcctgcgg cgcaaggcgc tgccagcgtg atcggccagc aggccggctt
     2101 gccgtggccg ctgctggtcg cggtgccggc gctggcgctg ctgctcgggg ggctggtagc
     2161 cgccctgcgc cggccggaca tcgtcgtgcg cgattcaccg cgtttcggcg aggcgctcga
     2221 catctggtac ccgtatctgc tcagccacca ccggccgacg ccccggacgc tgaagcgcta
     2281 ccagaaccgc atccgctacg tcgcgatgcg ccagcgcccg gccgagcctg cgaccgatcc
     2341 gctagagcgc ctgctgcgcc gctggctggg ggtgc
//
LOCUS       DAOQJH010000484         2371 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_33407_length_2371_cov_3.265112, whole genome shotgun sequence.
ACCESSION   DAOQJH010000484 DAOQJH010000000
VERSION     DAOQJH010000484.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2371)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2371)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2371
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33407_length_2371_cov_3.265112"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..470)
                     /locus_tag="PLQ18_10655"
     CDS             complement(<1..470)
                     /locus_tag="PLQ18_10655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02157.1"
                     /translation="MEQPPRPPDAPPPAPPSGAARQQALLGWAGIAFALMALACFVGI
                     GIYLLGSAREVRLDGVTSREALLVLAFPPLALFVTGLCCAGFGYLLLNAAGAVGRTVI
                     PAQDYRLVSALVEAEKEHGIELYIRLSSLNGLTGVFTRLGITGLPLATITLTLL"
     gene            820..1554
                     /locus_tag="PLQ18_10660"
     CDS             820..1554
                     /locus_tag="PLQ18_10660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015145082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dinitrogenase iron-molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HNE02158.1"
                     /translation="MSESEVEPITQEAALRVALAARAMPNIALAQLVQVLHNRLGDHI
                     DTDKLRTVTVTDLKTGLGSVDGEEDNEDILIGLDAMKMAVRILWGDDDGAADLPPVES
                     YAEGDMPGSVRIALASDSGTTLNGHFGSCLRFLVYQVSPTESRLVGIRDALEADFAED
                     KNAFRTSLISDCHVLYVVSIGGPAAPKVIRADIYPIKRPEGGEAAEVIAQFQQAMIKS
                     PPPWLSKILGVAAGQRVKNYDAGTRA"
     gene            1602..1928
                     /locus_tag="PLQ18_10665"
     CDS             1602..1928
                     /locus_tag="PLQ18_10665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02159.1"
                     /translation="MTEKDRFEAATEMVATAIQTAGVFGENQRITRLIVGNLGRMAAE
                     LDAEPGSPGGRALIRHALAGIDAAEAALVPKLIEGWEKIDEIGVEEVHYEGPGHIQPV
                     YILRNT"
     gene            complement(1962..2369)
                     /locus_tag="PLQ18_10670"
     CDS             complement(1962..2369)
                     /locus_tag="PLQ18_10670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017952984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SxtJ family membrane protein"
                     /protein_id="HNE02160.1"
                     /translation="MDRHEEVVGSSDRSFGFVFAGVFTVLTLVKLWRGWTDWGYLWLG
                     LAIAFALAAAAAPSVLAPLNRIWLKIGLALHKVMTPLIMGLLFFLVVTPIGLIMRATG
                     KDLLRLKRTGAEKSYWIDRTPPGPAPDTMKNQF"
BASE COUNT          409 a          810 c          828 g          324 t
ORIGIN      
        1 aacagcagcg tcagcgtgat ggtcgcgagc ggcaggccgg tgatgccgag gcgcgtgaac
       61 acgccggtca ggccgttgag gctgctcagg cggatgtaca gctcgatgcc gtgctccttc
      121 tcggcctcga ccagcgcgct gaccagccgg tagtcctgcg ccgggatcac ggtgcgcccg
      181 accgccccgg cggcgttgag cagcaggtag ccgaagccgg cgcagcagag cccggtcacg
      241 aacagcgcca gcggcggaaa ggccagcacc agcagggcct cgcgcgaggt cacgccatcg
      301 agcctgacct cgcgcgcgct gccgagcaga tagatgccga tgccgacgaa gcaggcgagc
      361 gccatcagcg cgaacgcgat gccggcccag ccgagcaggg cctgctggcg ggcggccccg
      421 gaaggcggcg cgggcggggg ggcgtccggc gggcgcgggg gctgttccat cgtcatcgtc
      481 ctcggcagtc agagggagtg ataccgatca cacgtgaatg cgctgtttat ccccctctcc
      541 ccaacccctc ccccgcgagg ggggaggggc tgaaacgatc gggtgttcag ccgtgatggg
      601 tatgagggag tgctcgtgtc gctgacgtat agcgcccggc accggcgcgc gcgcgacctg
      661 tcgcaaaccc gacaggggcc gacaatgcgg gggcgcgagc ccggggcgtg gtttcctgat
      721 gaggatcagg ttgttacgca gtcgtgccgg actggcatcg cctgtgcagt gcagcatttc
      781 cggtacgacc gccccctcac tgcccccgga gatgccgcga tgtccgagtc cgaagtcgag
      841 cccatcacgc aggaagccgc gctgcgcgtc gccctcgctg cgcgcgccat gccgaacatc
      901 gcgctggcgc agctggtgca ggtgctgcac aaccgcctcg gcgatcacat cgacaccgac
      961 aagctgcgca ccgtcaccgt caccgacctc aagaccggcc tcggcagcgt cgacggcgag
     1021 gaggacaacg aggacatcct gatcggcctc gacgcgatga agatggccgt gcgcatcctc
     1081 tggggcgacg acgacggcgc cgccgacctg ccgccggtcg agtcctacgc cgagggcgac
     1141 atgcccggct cggtgcgcat cgcgctcgcc tcggacagtg gcacgacgct gaacggccac
     1201 ttcggctcct gcctgcgctt cctcgtctat caggtctcgc cgaccgagag ccggctggtc
     1261 ggcatccgcg acgcgctcga agccgacttc gccgaggaca agaatgcctt ccgcacctcg
     1321 ctgatcagcg actgccacgt gctgtacgtg gtgtcgatcg gcggcccggc ggcgccgaag
     1381 gtgatccgcg ccgacatcta tccgatcaag cgtcccgaag gcggcgaggc cgccgaggtc
     1441 atcgcgcagt tccagcaggc gatgatcaag tcgccgccgc cgtggctgtc gaagatcctc
     1501 ggcgtcgccg ccgggcagcg cgtgaagaac tacgacgccg gcacgcgcgc ctgatggccg
     1561 cggccgcagg tcgccggcag gcgccggcga cggagacggg catgaccgaa aaagaccgct
     1621 tcgaggcggc cacggaaatg gttgccaccg cgatccagac cgccggcgtg ttcggcgaga
     1681 accagcgcat cacgcggctg atcgtcggca acctcgggcg catggccgcc gaactcgacg
     1741 ccgaaccggg cagccccggc ggccgggcgc tgatccgcca tgcgctggcc ggcatcgatg
     1801 cggccgaagc agcgctggtg ccgaagctga tcgaaggctg ggagaagatc gacgagatcg
     1861 gcgtggagga agtccactac gaaggcccgg gccatatcca gcccgtctat attcttcgca
     1921 acacctaggg gaagggcgga ctgccgcagc gggtcctgcg gtcagaactg gtttttcatc
     1981 gtgtcgggcg cggggcccgg cggggtccgg tcgatccagt agctcttttc cgcgcccgtc
     2041 cgcttcagcc gcagcaggtc cttgccggtg gcgcgcatga tcagcccgat cggcgtcact
     2101 accaggaaga acagcaggcc catgatcagg ggcgtcatca ccttgtgcag cgccaggccg
     2161 atcttcagcc agatccggtt gagcggggcg aggacggacg gcgcggcggc ggcggccagc
     2221 gcgaaggcga tcgcgaggcc cagccagagg tagccccagt cggtccagcc ccgccacagc
     2281 ttcaccagcg tcaggacggt aaaaacgcca gcgaaaacaa agccgaacga ccgatcggag
     2341 ctgccgacga cctcttcgtg ccggtcgatt c
//
LOCUS       DAOQJH010000485         2369 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_33462_length_2369_cov_3.755402, whole genome shotgun sequence.
ACCESSION   DAOQJH010000485 DAOQJH010000000
VERSION     DAOQJH010000485.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2369
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33462_length_2369_cov_3.755402"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(89..1036)
                     /locus_tag="PLQ18_10675"
     CDS             complement(89..1036)
                     /locus_tag="PLQ18_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018866733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide-inducible genes activator"
                     /protein_id="HNE02161.1"
                     /translation="MTLTELRYIVAVARERHFGRAAEACFVSQPTLSVAVRKLEDELG
                     VVLFERGPGEVTVTPVGRRVVGQAQRVLEEADAIRALALSGKDELAGVLRLGAIYTIG
                     PYLLPQLIPRLKRVAPHMPLQIEENYTAVLGERLKQGKLDVLILSLPFEESGVLTEAL
                     YEEPFVVVMPAGHALEHERAIDVHALAQEDLLLLGPGHCFRDQVLQVCPECNRAAVAS
                     DADNMQRTLEGSSLETIRLMVATGMGVTVLPQTSMTAYASAPELLSVRPFTEPAPKRV
                     VALAWRKSFPRPAAIRVLAAAVRACPLGESIEVLEGATA"
     gene            complement(1100..1402)
                     /locus_tag="PLQ18_10680"
     CDS             complement(1100..1402)
                     /locus_tag="PLQ18_10680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02162.1"
                     /translation="MTMRTSIPGIVAGLALTGMAAAAVAADGAWIPRGKPMRQFPSCY
                     QMWVCVAKDKPEGPFSGMPRGTWGACDTSYTAGAAGSQAESCEKCLAGPPEDACKP"
     gene            1975..>2369
                     /locus_tag="PLQ18_10685"
     CDS             1975..>2369
                     /locus_tag="PLQ18_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HNE02163.1"
                     /translation="MAYQHIKMPATGEKITVVDGKLSVPDQPVVGYVEGDGIGPDITR
                     ACLRVWDAAVEQAYDGKRKIHWCELFMGEKAAEIYDGNYFPDETLEALKDLIVSIKGP
                     LTTPVGGGFRSLNVALRQELDLYACVRPVR"
BASE COUNT          344 a          851 c          827 g          347 t
ORIGIN      
        1 gatcgaaggc ttgcaggcgc tcgatcgcga ccccggctga agcctgcgcg ccggccagcg
       61 gccgggacgg ggcggcgacg ggccggattc aggccgtcgc gccctccagc acctcgatgg
      121 actcgccgag cggacaggcg cgcacggccg ccgccagcac gcggatcgcc gccggccgcg
      181 ggaagctctt gcgccaggcc agcgcgacga cgcgcttcgg cgccggttcc gtgaacggcc
      241 gcacgctcag cagttcgggc gcgctggcgt aggcggtcat cgaggtctgc ggcagcacgg
      301 tcacgcccat gccggtggcg accatcagcc ggatggtttc gagcgagctg ccttcgagcg
      361 tgcgctgcat gttgtcggcg tccgaggcca ccgcggcgcg gttgcactcg gggcagacct
      421 gcagcacctg gtcgcggaag cagtggccgg ggccgagcag cagcagatcc tcctgcgcga
      481 gcgcgtgcac gtcgatcgcg cgctcgtgct ccagcgcgtg gccggccggc atcacgacga
      541 cgaagggctc ctcgtacagc gcctcggtca gcacgccgct ctcctcgaag ggcagcgaca
      601 ggatcagcac gtcgagcttg ccctgcttca ggcgctcgcc gagcacggcc gtgtagtttt
      661 cctcgatctg cagcggcatg tgcggcgcga cgcgcttcag gcgcgggatc agttgcggca
      721 gcaggtaggg gccgatcgtg tagatcgcgc cgaggcgcag cacgccggcg agctcgtcct
      781 tgcccgacag cgccagcgcc cggatcgcgt cggcttcctc cagcacgcgc tgcgcctgcc
      841 cgaccacgcg ccggccgacc ggcgtgacgg tgacctcgcc ggggccgcgc tcgaacagca
      901 cgaccccgag ttcgtcctcg agcttgcgca ccgccaccga cagcgtcggc tggctgacga
      961 agcaggcctc ggcggcacgg ccgaagtggc gttcgcgtgc caccgcgacg atgtagcgca
     1021 gttcggtcag cgtcatgtac gggcttcctc ggctgagaac gccgccgcag ccgcagccgg
     1081 tgggcgatcc gggccggcgt cagggcttgc aggcgtcctc gggcgggccg gcgaggcatt
     1141 tctcgcagct ttcggcctgg ctgccggccg cgccggcggt ataactggtg tcgcaggcgc
     1201 cccaggtgcc gcgcggcatg ccgctgaacg gtccctcggg cttgtccttc gcgacgcaga
     1261 cccacatctg gtagcaggac ggaaactgcc gcatcggctt gccgcgcggg atccacgcgc
     1321 cgtcggccgc gacggccgcg gcggccatcc cggtgagggc cagtccggcg acgattccgg
     1381 ggatcgaggt gcgcatcgtc atcttgcggt cgctctcagg cgggtcggtc cgccgttcct
     1441 ggccgaccgg agcaccgatg gtagccgcct gccggcgccc gcaacaagaa atccgcgccc
     1501 cgcacgcgcc gcgcccgccg gtttcacctg ccggaaaagc ccctgcctcc ggcaatcccc
     1561 gctgcgcgcc ccgaaatcgc cattcggccc cggcccgccg ccggaggcgg ccggcggcgc
     1621 gattgccacc ccgcacgttt gcgtgtagtt ttgcgcgcgg ctggagcacc cggcatccat
     1681 aacgagatcg acacagatcg acaaggccgg gcaaggggtt ggcagcgttt cggcgtaccg
     1741 tattcgtggc tcccgcggcc gtttccggct gcgagccttc ctccgcaggc gacagacccg
     1801 tccggcagct ccggcctgcc ggtgcgacct gcgcgtttgc tgtacggacc atcccgccgc
     1861 aacgtttccg gccccgaaca tcgggggtcg cgacaagtca tcaggtcttt ccagcctgac
     1921 gcgccgcgtg aacaagtctg tccgccgcag cgagcggacg aggaaggagg tctcatggct
     1981 taccagcaca tcaagatgcc cgccaccggc gagaagatca ccgtcgtcga cggcaagctc
     2041 agcgttccgg accagccggt cgtcggctac gtcgagggcg acggcatcgg ccccgacatc
     2101 acccgcgcct gtctgcgcgt gtgggatgcg gcggtggagc aggcctacga cggcaagcgc
     2161 aagattcact ggtgcgagct gttcatgggc gagaaggctg ccgagatcta cgacggcaac
     2221 tacttcccgg acgaaaccct ggaagccctg aaggacctga tcgtctcgat caagggtccg
     2281 ctgaccacgc cggtcggtgg cggcttccgc tcgctgaacg tcgcgctgcg ccaggaactc
     2341 gacctgtatg cctgcgtgcg cccggtgcg
//
LOCUS       DAOQJH010000486         2366 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_33546_length_2366_cov_3.279965, whole genome shotgun sequence.
ACCESSION   DAOQJH010000486 DAOQJH010000000
VERSION     DAOQJH010000486.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2366
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33546_length_2366_cov_3.279965"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..495
                     /locus_tag="PLQ18_10690"
     CDS             <1..495
                     /locus_tag="PLQ18_10690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010961260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNE02164.1"
                     /translation="DLLPLVEGCTVSTKYGAVKTDHILFIASGAFHLAKPSDLVPELQ
                     GRLPIRVELDALSAQDFVRILTEPHASLTEQYKALLATEGFGVTFAPDAVQRLAEIAH
                     SVNERTENIGARRLHTVLERLLEDVSFEAADRDGQTLLIDAAYVDAHLGQLVQDEDLS
                     RFIL"
     gene            619..1068
                     /locus_tag="PLQ18_10695"
     CDS             619..1068
                     /locus_tag="PLQ18_10695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02165.1"
                     /translation="MLVPALALALLGGCTIPLPAWLGGGGGGPPSPAKPAAGVVAPLS
                     IDDAGLLATRERRLELGLRLSSRSGETQWVSVRFQTPDGQDCVSLKELPAQGRLLFTC
                     AQARLVAELDYPVTIESWRDAGLQSAFAPIRTTLRFTRNDLVAAGVR"
     gene            complement(1082..1852)
                     /locus_tag="PLQ18_10700"
     CDS             complement(1082..1852)
                     /locus_tag="PLQ18_10700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012613435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HNE02166.1"
                     /translation="MLGDHPLRFESSAVTHVGMVRKVNEDAVLDQSGRGMWAVADGMG
                     GHAAGDFASRTIVQTLSRLPPAPTISVYVRSVQTSLQHINELLRQEAARRERETIGAT
                     VAVLVAAADELACLWAGDSRLYRFRRPRLEQLTRDHSYVQELVDAGALSPEEAQRHPM
                     GNVVTRAVGAHETLEVDAHPVITRPGDVLLLCTDGLSKVVSDDEIAQVLATRRPAEAT
                     RELVNLALLRGAPDNVSLVVVRVDADTGTVTQPVTPVV"
     gene            complement(1926..2174)
                     /locus_tag="PLQ18_10705"
     CDS             complement(1926..2174)
                     /locus_tag="PLQ18_10705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02167.1"
                     /translation="MFGHFEDLADAGSMAGRLAQQRLRPDWRGSSLWWTLGSERIEPC
                     VILAEGLPPAGTLGSFFDGDFARAGYAAGDTAGSTHDQ"
     gene            2179..>2366
                     /locus_tag="PLQ18_10710"
     CDS             2179..>2366
                     /locus_tag="PLQ18_10710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HNE02168.1"
                     /translation="MPGSANAIPAVTHLRHLAAIEGEHVWEAELETGRTHQIRAHVAS
                     LGFPIVGDHRYGPGGRTTG"
BASE COUNT          330 a          837 c          840 g          359 t
ORIGIN      
        1 gacctgctgc cgctggttga aggctgcacg gtgtcgacca agtacggagc cgtgaagacc
       61 gaccacatcc tgttcatcgc ctcgggcgcc ttccatctgg ccaagccctc ggatctggtg
      121 ccggagctgc agggtcgcct gccgatccgc gtcgaactcg atgccctgag cgcgcaggac
      181 ttcgtgcgca tcctgaccga gccgcacgcc tcgctgaccg agcagtacaa ggccctgctc
      241 gcgaccgagg gcttcggcgt caccttcgcg cccgacgccg tgcagcgcct cgccgagatt
      301 gcccacagcg tcaacgagcg caccgagaac atcggcgcgc gccggctgca caccgtgctc
      361 gaacggctgc tggaggatgt ctcgttcgaa gccgcggacc gcgacggcca gacgctgctc
      421 atcgacgcgg cctacgtcga tgcgcacctc ggccagctgg tgcaggacga ggatctcagc
      481 cgcttcatcc tctgacgcat ggcgcgatcc gaagcccggc gggcgcttat agtgggcgcc
      541 cgctgccgtt tccggatgcc gccaccgatg cccgtcccga ctgtcccttg ccctgtccgc
      601 cgggtccgcg cccgtgcggt gcttgtcccc gccctcgcgc tcgccctgct cggcggctgc
      661 acgatcccgc tgccggcctg gctcggtggc ggtggcggcg gaccgccgtc accggcgaag
      721 ccggcggccg gggtggtggc accgctgtcg atcgacgatg ccggcctgct cgcgacgcgc
      781 gagcggcggc tcgaactggg gctgcggctg tcgagccgtt cgggcgagac gcagtgggtg
      841 tcggtgcgtt tccagacgcc cgacggccag gactgcgtga gcctgaagga gctgccggcg
      901 cagggccggc tgctgttcac ctgcgcgcag gcacggctcg tcgccgaact cgattacccg
      961 gtcacgatcg agagctggcg tgacgccggc ctgcagagcg ccttcgcgcc gatccgcacg
     1021 acgctgcgct tcacgcgcaa cgatctggtc gccgccggcg tgcgctgacg ggcgcgcgtg
     1081 ctcagacgac cggcgtcacc ggctgcgtga ccgtgccggt gtcggcgtcg acgcgcacga
     1141 cgacgaggct gacgttgtcg ggcgcgccgc gcagcagcgc cagattgacg agttcacggg
     1201 tggcctccgc cggccggcgg gtggcgagca cctgcgcgat ctcgtcgtcg ctgacgacct
     1261 tgctcaggcc gtcggtgcag agcagcagca cgtcgccggg gcgggtgatc accgggtgag
     1321 catcgacctc cagcgtctcg tgggcaccga cggcgcgcgt gacgacgttg cccatcgggt
     1381 ggcgctgggc ttcctccggg ctcagggcgc cggcatcgac gagttcctga acgtagctgt
     1441 gatcgcgcgt cagctgttca agcctcggcc ggcggaagcg gtacaggcgg ctgtcaccgg
     1501 cccacagaca ggccagttcg tcagcggcgg cgaccagcac cgcgaccgtc gcgccgatgg
     1561 tctcgcgctc gcggcgtgcg gcttcctgac gcagcagctc gttgatgtgc tgcaggctgg
     1621 tctgcaccga gcggacatag acgctgatcg tcggcgccgg cggcaggcgc gacagcgtct
     1681 gcacgatggt ccgggacgcg aaatcaccgg ctgcgtgacc gcccatgcca tcggccacgg
     1741 cccacatgcc gcggcccgac tggtcgagca cggcatcctc gttgaccttg cggaccatgc
     1801 ctacgtgcgt gacggcgctc gattcgaagc gcaaggggtg atcgccgagc atcgcgctgt
     1861 ccccggtgtc gtcggtgcga ctggaaggta gtggcagcgg cgcgtcggac ggtaacaagt
     1921 tgtcatcatt gatcatgcgt ggatcctgcg gtgtcgcccg cggcatagcc ggcccgggcg
     1981 aagtccccgt cgaagaagct gccgagcgtg ccggcaggcg gcaggccctc ggccagaatc
     2041 acgcagggct cgatgcgctc cgagcccagc gtccaccaca gcgagctgcc gcgccagtcc
     2101 gggcgcaggc gctgctgcgc gagccggccg gccatgctgc cggcatcggc gaggtcttcg
     2161 aagtgcccga acatcggcgt gcccggcagc gccaacgcga tcccggccgt cacgcacctg
     2221 cgccatctcg cggcgatcga gggcgagcat gtgtgggagg ccgagctcga gacgggtcgt
     2281 acgcaccaga tccgcgcgca cgtggcctcg ctcgggtttc ccatcgtcgg cgatcatcgc
     2341 tacgggcccg gcggacgaac gacggg
//
LOCUS       DAOQJH010000487         2361 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_33659_length_2361_cov_3.793582, whole genome shotgun sequence.
ACCESSION   DAOQJH010000487 DAOQJH010000000
VERSION     DAOQJH010000487.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2361
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33659_length_2361_cov_3.793582"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..487)
                     /locus_tag="PLQ18_10715"
     CDS             complement(<1..487)
                     /locus_tag="PLQ18_10715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phytoceramidase"
                     /protein_id="HNE02169.1"
                     /translation="MHDRDTFGNLLLFALIALQLGGPALPFLAQPPGYHAFADTRALA
                     GLANAADVLSNLGFLLAALAGAWTLLRLPATATAVARERDQRRACAVFFAGLALTAAG
                     SAYYHLAPDDGRLVWDRLPMTIVFAGAFASVGAAKYPRVWSARLLVLMLLSAPMSVVW
                     WA"
     gene            complement(683..>2361)
                     /locus_tag="PLQ18_10720"
     CDS             complement(683..>2361)
                     /locus_tag="PLQ18_10720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010962290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNE02170.1"
                     /translation="KPALARGELHCIGATTLDEYRQYIEKDAALERRFQKVLVDEPSV
                     EDTIAILRGLKERYEVHHKVDITDPAIVAAAQLSHRYITDRQLPDKAIDLIDEAASRI
                     RMEMDSKPEAMDRLDRRLIQLKMERVALKKENDDASRKRLDALETEVTRLEREYADLD
                     EVWKAEKAMLTGAADIKAELEKARLDFDAAKRSGDLNRMSELQYGRIPELERRMKAAE
                     AGESHDFQLLRNRVTDEEIAEVVSKWTGIPVAKMLEGEREKLLRMEDVLGRRVVGQAE
                     AVRAVADAIRRSRAGLADPNRPNGSFLFLGPTGVGKTELCKALASFLFDTEEALVRID
                     MSEFMEKHSVARLIGAPPGYVGYEEGGYLTEAVRRKPYSVILLDEIEKAHADVFNVLL
                     QVLDDGRLTDGQGRTVDFRNTVVVMTSNLGSDTIMEMAGEDNYGAMKAAVMAILAGHF
                     RPEFLNRVDETVVFHPLGREQIRSIAAIQIEGLRKRLAARDIALEVGAGALDKLGEAG
                     FDPVYGARPLKRTIQQMLENPLAKRILAGEFGPGDTVLVEPDLSLRRRAA"
BASE COUNT          345 a          836 c          809 g          371 t
ORIGIN      
        1 ccgcccacca caccacgctc atcggcgctg acagcagcat cagcaccagc agccgggcgc
       61 tccagacccg cgggtacttg gcggccccga ccgacgcgaa ggcgccggca aagacaatcg
      121 tcatcggcag ccggtcccag accagccggc catcatcggg cgcgaggtgg tagtacgcgg
      181 agccggccgc cgtcagcgcc agtccggcga agaacaccgc gcaggcccgg cgctggtcgc
      241 gttcgcgggc cacggccgtg gccgtggccg gcagccgcag cagcgtccac gcgccggcca
      301 gcgccgcgag caggaagccg agattcgaca gcacgtcggc cgcattggcg aggccggcga
      361 gcgcgcgcgt gtcggcgaag gcgtgatagc cgggcggctg ggccagaaac ggcagcgccg
      421 ggccgccgag ctgcagcgcg atcagcgcga acagcagcag gtttccgaag gtatcccgat
      481 cgtgcatcgc cttcctcact ccgtgcatcc ggggggttcc gggggccggc cgtcgtggcg
      541 cggtgaggcg cgatgattcc gaagccgccg gacgacggca agcgccggtg cgggcgcgac
      601 ggggcgcctg caccggccgc ccgccgcaaa gcaaaacccc tgccgcgcgc tgcggcaggg
      661 gtctgcaggc acaagaggat gctcaggccg cgcgccggcg cagcgacagg tccggctcga
      721 ccagcaccgt gtcgcccggc ccgaactcgc cggcgaggat gcgcttcgcc agcgggttct
      781 ccagcatctg ctggatcgtg cgcttcagcg gccgcgcgcc gtagaccgga tcgaaaccgg
      841 cttcgccgag cttgtcgagc gcaccggcac cgacctcgag cgcgatgtcg cgcgccgcga
      901 ggcgcttgcg caggccctcg atctggatcg ccgcgatcga gcggatctgc tcgcgcccga
      961 gcgggtggaa cacgacggtc tcgtcgacgc ggttcaggaa ctccggccgg aagtgcccgg
     1021 cgaggattgc catcaccgcc gccttcatcg cgccgtagtt gtcctcgccg gccatctcca
     1081 tgatcgtgtc ggagccgagg ttcgaggtca tcacgacgac ggtgttgcgg aagtccacgg
     1141 tccgcccctg accgtcggtc aggcgtccgt catcgagcac ctgcagcagc acgttgaaga
     1201 catcggcgtg cgccttctcg atctcgtcga gcaggatcac gctgtagggc ttcctgcgca
     1261 cggcctcggt caggtagccg ccttcctcgt aaccgacgta gcccgggggc gcaccgatca
     1321 ggcgcgcgac cgagtgcttc tccatgaact cgctcatgtc gatgcgcacc agcgcttcct
     1381 cggtatcgaa caggaagctc gcgagcgcct tgcagagctc ggtcttgccg acgccggtcg
     1441 gcccgaggaa caggaagctg ccgttcggcc ggttcggatc ggcgagcccg gcgcgcgagc
     1501 ggcggatggc gtccgccacc gcacgcacgg cctcggcctg accgacgacg cgccggccga
     1561 gcacgtcttc catgcgcaag agcttctcgc gctcgccttc gagcatcttc gcgaccggga
     1621 tgccggtcca cttgctgacg acctcggcga tctcctcgtc ggtgacgcgg ttcctcagca
     1681 gctggaagtc gtgcgactcg ccggcctcgg ccgccttcat gcgccgctcc agctccggga
     1741 tgcgcccgta ctgcagctcc gacatgcggt tcagatcgcc cgaacgcttc gccgcatcga
     1801 agtcgagccg cgccttttcg agttcggcct tgatgtcggc cgcgccggtc agcatggctt
     1861 tttcggcctt ccagacctcg tcgagatcgg cgtactcgcg ctccaggcgc gtgacctcgg
     1921 tctccagcgc gtcgaggcgc ttgcgcgagg catcgtcgtt ttccttcttc agggccacgc
     1981 gctccatctt cagctggatc agccgccggt cgagccggtc catcgcctcg ggcttggagt
     2041 ccatctccat gcggatgcgc gaggccgcct catcgatcag gtcgatggcc ttgtccggca
     2101 gctggcggtc ggtgatgtag cggtgcgaaa gctgggccgc ggcgacgatc gccgggtcgg
     2161 tgatgtcgac cttgtggtgg acctcgtagc gctccttcag gccgcgcagg atggcgatgg
     2221 tgtcctcgac cgaaggctcg tcgaccagca ccttctggaa gcggcgttcg agcgcggcat
     2281 ccttctcgat gtactggcgg tactcatcga gcgtggtggc gccgatgcag tgcagctcgc
     2341 cgcgcgccag cgccggcttg a
//
LOCUS       DAOQJH010000488         2356 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_33803_length_2356_cov_3.714907, whole genome shotgun sequence.
ACCESSION   DAOQJH010000488 DAOQJH010000000
VERSION     DAOQJH010000488.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2356
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33803_length_2356_cov_3.714907"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..885
                     /locus_tag="PLQ18_10725"
     CDS             <1..885
                     /locus_tag="PLQ18_10725"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNE02171.1"
                     /translation="NGQEVQGFNFIVPSNTAREYLGPAGTTNTAGLVDQGWNAGLEQY
                     WAGHFRTAKTKFEEVVSLQPNHAEARRLITETQERILRGEEKPDAPAPAPLPIALTPP
                     APGPATAPVPTPAPAADGTGTTLIIAIAAIAVLIVIAVAVLLLRNRGGGRPAPTTRAP
                     YAGPSLRGGTAHLPAYAGDSAATMFGAPGGSTGEARLNFTAGPLSGQNISIPASGAWL
                     GRDASRADILVSHPMVSGQHLWIGRQGERWLLKDKGSTNGTYIGSSGSGRVSEHPLRN
                     GDRVLIAPDGAVSFTVSL"
     gene            902..1486
                     /locus_tag="PLQ18_10730"
     CDS             902..1486
                     /locus_tag="PLQ18_10730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014778519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HNE02172.1"
                     /translation="MSVPATRLSLAAFLDWEATQPERHEYVRGEVFAMTGASDRHNLV
                     AGNLYMLLRTHLRGSPCQVFISDMKVRVDAADAIFYPDLLVTCSASDRSDRLAKRDPV
                     VIVEVLSPSTAGYDLSAKFAHYRRLASLQEYVTIDPDAPLVQVFRRSPDGWTLHAAEA
                     GDTVGFASIDLCVPIAAVYEDVRFEPPADGGSRA"
     gene            1745..>2356
                     /locus_tag="PLQ18_10735"
     CDS             1745..>2356
                     /locus_tag="PLQ18_10735"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008438608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNE02173.1"
                     /translation="MRPASLNERLPRRQAAGLPPDGIALRDGACLFFKSTARTPPVKF
                     RPDIEGLRAVAVMLVVAAHARIDFLAGGFIGVDVFFVLSGYLITGLLVQELTTHGRID
                     LPRFYARRFRRLFPSLLVVIAISSLLGQQLLAIGEQPGQALAAASAIVWLSNFYFSFS
                     ELDYFSAGAEGNLFLHTWSLGVEEQFYLIWPALLILLATAGRPD"
BASE COUNT          370 a          854 c          754 g          378 t
ORIGIN      
        1 aacggtcagg aagtgcaggg cttcaacttc atcgtgccgt cgaacaccgc gcgcgaatac
       61 ctcggtccgg ccggcaccac caacaccgcc ggtctggtcg atcagggctg gaacgccggg
      121 ctggagcagt actgggccgg acacttccgc accgccaaga ccaagttcga ggaagtcgtc
      181 tcgctgcagc cgaaccacgc cgaggcgcgc cggctgatca ccgagaccca ggaacgcatc
      241 ctgcgcggcg aggaaaagcc cgatgccccg gcaccggccc cgctgccgat cgcactgacg
      301 cccccggcgc cgggcccggc aacagcgccg gtgccgacgc ccgcaccggc cgcagacggc
      361 accggcacga cgctgatcat cgcgatcgcc gccatcgccg tgctgatcgt gatcgcggtc
      421 gccgtgctgc tgctgcgcaa ccgcggcggc ggccggccgg caccgacgac gcgcgccccc
      481 tacgccggcc cgtcgctgcg cggcggcacc gcccacctgc cggcctacgc cggcgacagc
      541 gccgcgacca tgttcggcgc tccgggcggc agcaccggcg aggcacggct caacttcacc
      601 gccggtccgc tcagcggcca gaacatcagc atcccggcca gcggcgcctg gctcgggcgc
      661 gacgccagcc gcgccgacat cctggtctcg cacccgatgg tctcgggcca gcacctgtgg
      721 atcggccgtc agggcgaacg ctggctgctg aaggacaagg gctcgaccaa cggcacctac
      781 atcggcagct ccggcagcgg ccgggtcagc gagcatccgc tgcgcaacgg cgaccgcgtg
      841 ctgatcgccc cggacggcgc ggtcagcttc acggtcagcc tgtgatcccg ggagaagccc
      901 gatgtccgtt cccgccaccc gcctgagcct cgccgccttc ctcgactggg aggcgacgca
      961 gcccgaacgc cacgagtacg tgcgcggcga agtgtttgcg atgaccggcg ccagcgaccg
     1021 ccacaacctg gtcgccggca atctgtacat gctgctgcgc acacacctgc gtggctcgcc
     1081 gtgtcaggtc ttcatcagtg acatgaaggt ccgtgtcgac gccgccgacg cgattttcta
     1141 tccggacctg ctggtcacct gcagcgccag cgatcgcagt gatcgtctgg ccaagcgcga
     1201 ccctgtcgtc atcgtcgaag tcctgtcgcc gtcgacggcc ggctacgacc tcagcgccaa
     1261 gttcgcgcac taccggcggc tcgcgagcct gcaggaatac gtgaccatcg acccggacgc
     1321 accgctggtg caggtcttcc gccgcagccc cgatggctgg acgctgcacg ccgccgaggc
     1381 cggcgacacg gtcggattcg ccagcatcga tctgtgcgtg ccgatcgcgg cggtgtacga
     1441 ggacgtgcgc ttcgagccgc cggcggatgg cggatcccgg gcctgaagcc gcggcacacc
     1501 ctcgcgcctg cacgctgccg gcgtttcagg cagaacccgc cttgcccgat gcgtcatgcc
     1561 gatcacacgc aagtgcatgg ttttcctctt ttctccccga ctcctcttcc acgaggcacg
     1621 agggactgaa aagcgaaggc ttgcggtgtg agcggtatcc gccatcgggc atccgcatgc
     1681 gcccttcgga aagaccggtt acggcgcgct gcggctgcag cgtaaaatcc cggggccggg
     1741 cagcgtgcgc ccggcttccc tgaacgaacg cctgccccgg aggcaagcgg cgggattgcc
     1801 gccagacggc atcgccctgc gcgatggcgc ctgcctgttt ttcaaatcga cagcaaggac
     1861 accacccgtg aaatttcgcc ccgacatcga aggattgcgt gcggtcgcgg tgatgctggt
     1921 cgtggcggca cacgccagga tcgattttct ggccggaggc ttcatcggcg tcgacgtgtt
     1981 tttcgtgctg tccggttacc tgatcaccgg tctgctcgtg caggaactga cgacgcacgg
     2041 acgcatcgat ctgccacggt tctatgcccg ccgtttccgg cgcctgtttc cttcgctgct
     2101 ggtcgtcatt gccatcagca gcctgctcgg ccagcaattg ctggccatcg gcgaacagcc
     2161 ggggcaggcg ctggccgcag ccagcgcgat cgtctggctc agcaactttt atttttcgtt
     2221 ttccgaactc gattacttca gtgcgggagc cgagggcaat ctgttcctgc acacctggtc
     2281 gctgggcgtc gaggagcagt tctacctgat ctggcccgcg ctgctgatcc tgctggccac
     2341 ggccgggcgc cccgac
//
LOCUS       DAOQJH010000489         2342 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_34182_length_2342_cov_4.409270, whole genome shotgun sequence.
ACCESSION   DAOQJH010000489 DAOQJH010000000
VERSION     DAOQJH010000489.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2342)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2342)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2342
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34182_length_2342_cov_4.409270"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..866)
                     /locus_tag="PLQ18_10740"
     CDS             complement(<1..866)
                     /locus_tag="PLQ18_10740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009460182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HNE02174.1"
                     /translation="MHPIRMPLALVLALASAAAPAAPVLVNALAIAGDATDLSGLPAG
                     PNGNRLGGFASDLHYDRYSNTYYGIPDRGPGGGLLAYDTRVQRFTLNVDSATGAISNF
                     QLQSTTMLTAGGQPLNGLNPALLNASSATLGLSQDPEGFAVGPHGTYFVSDEYGPAVR
                     EFSAGGELIRSFTTPANLIPRDSGGGVNYVDGRPVIVSGRQDNRGFEGLTLSPDGTKL
                     YAMLQDPLVDEGSSSDGRRSRNLRLVEFDVATGLATKQMIYRLEDRDDINARIPGTAD
                     DFSATNQGRSIG"
     gene            1094..2197
                     /gene="aroC"
                     /locus_tag="PLQ18_10745"
     CDS             1094..2197
                     /gene="aroC"
                     /locus_tag="PLQ18_10745"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008249968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HNE02175.1"
                     /translation="MSGNTYGKLFTVTTAGESHGAALVGIVDGCPPGLELSEADLQVD
                     LDRRKPGTSRHTTQRREDDVVRILAGVFEGRTTGCPIALLIENTDQRSKDYSKIADRF
                     RPGHADYTFHHKFGLRDHRGGGRSSARETAMRVAAAGIAKKWLRARHGVEVRGYLSQL
                     GPLRAQALDWNEVGNNPFFCPDAALVPQLESYMDALRKSGDSVGARVTVVASGVPVGL
                     GEPIFDRLDAELAHALMSINAVKGVEIGDGFGCVEALGTQFRDELTPAGFLSNHAGGV
                     LGGISTGQDIVAHIALKPTSSIRLPGRSIDVDGNPVEVITTGRHDPCVGIRATPIAEA
                     MVALVLMDHVLRQRGQNADVRVTTPVIPGAAGG"
BASE COUNT          370 a          810 c          823 g          339 t
ORIGIN      
        1 atgccgatcg agcggccctg attggtcgcg gagaagtcat cggccgtgcc ggggatgcgg
       61 gcgttgatgt cgtcgcggtc ttccaggcgg tagatcatct gcttcgtcgc cagcccggtc
      121 gcgacatcga actcgacgag acgcaggttg cgcgagcggc ggccgtcgct gctgctgccc
      181 tcatcgacca gcgggtcctg cagcatcgcg tacagcttcg tgccatccgg gctcagcgtc
      241 aggccctcga agccgcggtt gtcctgacgg ccgctgacga tgaccggccg gccgtcgacg
      301 tagttgacgc cgccgccgct gtcgcgcggg atcaggttgg ccggggtcgt gaagctgcgg
      361 atcagctcgc cgcccgccga gaactcgcgc acggccggac catattcgtc cgagacgaaa
      421 tacgtgccgt gcggaccgac cgcgaagcct tccgggtcct gcgacaggcc gagcgttgca
      481 ctgctggcgt tcagcagcgc cggattcagg ccgttcagcg gctggccgcc ggccgtcagc
      541 atcgtcgtcg actgcagctg gaagttcgag atcgcgccgg tggcggaatc gacgttcagc
      601 gtgaagcgct gcacgcgcgt gtcgtaggcg agcagaccgc cgccggggcc gcggtccgga
      661 atgccgtaat aggtgttcga gtagcggtcg taatggagat cggacgcgaa accgccgagc
      721 cggttgccgt tcggaccggc cggcagcccg gagagatcgg tggcatcgcc ggcgatggcg
      781 agcgcattga cgaggacggg tgccgcgggc gcagcggcgg aagccagcgc aagcacgagc
      841 gccagcggca tacggatcgg atgcatggac agggttctct gcagcaaggg ggatggcggc
      901 atccctgccg tcacggccgc ttcccgacgg cgatctcctg ttcctgcgcg cagtctggtg
      961 cgggtgcatg acggtttcgt ggcagtgata cgacaccgga aaacgccgcc ggagccgcaa
     1021 aacacttggc acgcgccgca ccggccgcgg acaattgccg gctttcccga gccccagccc
     1081 gagcgcgtcc gccatgtccg gaaacaccta cggcaagctg ttcaccgtca ccacggccgg
     1141 cgaatcgcac ggcgccgcgc tggtcggcat cgtcgatggc tgcccgcccg ggctggagct
     1201 gtccgaggcc gacctgcagg tcgatctcga ccgccgcaag cccggcacca gccggcacac
     1261 gacgcagcgg cgcgaggacg atgtcgtgcg catcctcgcc ggcgtgttcg agggccgcac
     1321 caccggctgc ccgatcgcgc tgctgatcga gaacaccgac cagcgctcga aggactactc
     1381 gaagatcgcc gaccgcttcc gccccggcca cgccgattac accttccacc acaagttcgg
     1441 gctgcgcgac caccgcggcg gcgggcgctc gtcggcgcgc gagacggcga tgcgcgtcgc
     1501 cgccgccggc atcgccaaga agtggctgcg cgcgcgccac ggcgtcgagg tgcgcggcta
     1561 tctgagccag ctcggcccgc tgcgcgcgca ggcgctcgac tggaacgagg tcggcaacaa
     1621 cccgttcttc tgcccggatg cggcgctggt gccgcagctc gaaagctaca tggacgcgct
     1681 gcgcaagtcc ggcgattcgg tcggtgccag ggtcacggtg gtcgcgagcg gcgtgccggt
     1741 cggcctcggc gaaccgatct tcgaccggct cgatgccgaa ctcgcgcacg cgctgatgag
     1801 catcaacgcc gtcaagggcg tcgagatcgg cgacggcttc ggctgcgtcg aggcgctcgg
     1861 cacgcagttc cgtgacgagc tgacgccggc gggatttttg agcaaccacg ccggcggcgt
     1921 gctcggcggc atctcgaccg ggcaggacat cgtcgcgcac atcgcgctca aacccacctc
     1981 cagcatccgc ctgccggggc gcagcatcga tgtcgacggc aatccggtcg aagtcatcac
     2041 gaccggccgc cacgacccct gcgtcggcat ccgcgccacc ccgatcgccg aggcgatggt
     2101 cgcgctggtg ctgatggacc acgtgctgcg ccagcgcggc cagaacgccg acgtgcgcgt
     2161 gacgacgccg gtgattccgg gcgccgccgg cggctgaaac ggctcgcgcc ccgcactgca
     2221 cgcattggta tctacacaag ccagcggttc cgtgggcagg atggatcgac catcaccaac
     2281 ggccggacgt cgatctcccc tctccccttg cgggagaggg gccgggggag agggggagac
     2341 gc
//
LOCUS       DAOQJH010000490         2340 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_34241_length_2340_cov_4.363239, whole genome shotgun sequence.
ACCESSION   DAOQJH010000490 DAOQJH010000000
VERSION     DAOQJH010000490.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2340)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2340)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2340
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34241_length_2340_cov_4.363239"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            245..1498
                     /locus_tag="PLQ18_10750"
     CDS             245..1498
                     /locus_tag="PLQ18_10750"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008718 - D-amino-acid dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0055130 - D-alanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HNE02176.1"
                     /translation="MKIVVLGAGVVGTTTAWYLAQAGHQVIVLERQPGPALETSYANA
                     GQISPGYSAPWAGPGVPVKAIKWMFMEHAPLVIRPRINPAMWRWMWQMLMNCNAKDYE
                     LNKSRMMRLAEYSRDQFIRLRAETGLHYDDRQQGTLQVFRTQAQIDGAAKDIAVLKSL
                     GVPHEVLDRAGCVAAEPALALVSDKIQGGLRLPGDETGDCFQFTNRLAEMAAARGVEF
                     RYRTAIFGLERSGDEITAVLTNRGRETADAVVCALGSYSTKLMQELGYHIPVFPVKGY
                     SITVPVVDPEFAPVSTVMDETHKVAITRLGDRIRVAGTAELTGYDLQLTNERGNTVRH
                     VVTDLFPRGGDVSRAELWTGLRPNTPDGTPIIGRTPLKRLWLNTGHGTLGWTMCCGSA
                     RVLTDQIGGRTPDIDTEGLSMARYG"
     gene            1639..>2340
                     /gene="alr"
                     /locus_tag="PLQ18_10755"
     CDS             1639..>2340
                     /gene="alr"
                     /locus_tag="PLQ18_10755"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HNE02177.1"
                     /translation="MRPAHAVIDLAALRHNLARARAAAPGARAIAIVKADGYGHGAAR
                     LLPALADADMLGVACIEEALALRAAGATQPVLLLEGVFSADELPVCAEQDFEIAIHDP
                     LHVEWLERARLATPLRVWLKLDSGMHRLGVRPQQVAPLLARLRVCANVAPQLGVMSHL
                     ACADEPARAETAAQLETFAAATAGLPVRRALSNSAGVLAWPAAHHDWIRPGIMLYGAT
                     PMLGRTGPEDGLKPVM"
BASE COUNT          345 a          850 c          803 g          342 t
ORIGIN      
        1 ccgccggccc ggcctcgccg aatttccgga tacggccgcg gcgcaggctt tgcggtcgcg
       61 gggcgggcag tctcgaatct tcggggcatt ctgctgtgca tgaggttctg atccggaaaa
      121 tccactgcag tacggccggt aacatagtgc atgttgccgg tgccgacctt gtatgccgtc
      181 gaagccgccg gttcccctgt ccagtttcag ccggctgcgg gccggtcacg ggagtgtgtt
      241 tgccatgaag atcgtcgttc tgggtgccgg tgtcgtcggc acgaccaccg cctggtacct
      301 cgcgcaggcc ggccatcagg tcatcgtgct ggagcgccag cccggaccgg cgctggagac
      361 gagctacgcc aacgccggcc agatctcgcc cggctactcg gcgccgtggg ccggtcccgg
      421 cgtgccggtc aaggcgatca agtggatgtt catggagcat gcgccgctgg tgatccgccc
      481 gcgcatcaat ccggcgatgt ggcgctggat gtggcagatg ctgatgaact gcaacgcgaa
      541 ggattacgag ctcaacaagt cgcggatgat gcggctcgcc gagtacagcc gcgaccagtt
      601 catcaggctg cgcgccgaaa ccggcctgca ctacgacgac cgccagcagg gcacgctgca
      661 ggtgttccgc acgcaggcgc agatcgacgg tgcagccaag gacatcgcgg tgctgaagag
      721 cctcggcgtg ccgcacgagg tgctcgaccg ggccggctgc gtggccgccg agccggcgct
      781 cgcgctggtc agcgacaaga tccagggcgg cctgcgcctg cccggcgacg agaccggcga
      841 ctgcttccag ttcaccaacc gcctcgccga gatggccgcg gcgcgcggcg tcgaattccg
      901 ctaccgcacg gcgatcttcg ggctggagcg tagcggcgac gagatcaccg ccgtgctgac
      961 caaccgcgga cgcgagaccg ccgatgccgt ggtctgcgcg ctcggcagct attcgaccaa
     1021 gctgatgcag gaactcggct accacatccc ggtgttcccg gtgaagggct actcgatcac
     1081 cgtacccgtt gtcgatccgg agttcgcgcc ggtatcgacg gtcatggacg agacccacaa
     1141 ggtcgcgatc acgcgcctcg gcgaccgcat ccgcgtcgcc ggcaccgccg agctgaccgg
     1201 ctacgacctg cagctgacca acgagcgcgg caacaccgtg cgccacgtcg tcaccgacct
     1261 gttcccgcgc ggcggcgacg tgagccgcgc cgaactctgg accggcctgc gcccgaacac
     1321 gccggacggc acgccgatca tcggccgcac gccgctgaag cgcctgtggc tgaacaccgg
     1381 ccacggcacg ctcggctgga cgatgtgctg cggctcggcg cgcgtgctca ccgaccagat
     1441 cggcggacgc acgccggaca tcgacaccga agggctgtcg atggcgcgct acggctgagg
     1501 gcgttcaccg cctgccgggg cctgcgcatg cagtcccccg tcccgttcac gaaggcgcac
     1561 gataagcgtc gatgacattc cagcgtcgcc gccgcctgcg ggaggcgacg caccgtacct
     1621 gcccggagtc cctgcccgat gcgtcccgcc cacgccgtca tcgatctcgc cgcgctgcgg
     1681 cacaacctcg cccgtgcccg cgccgcggca ccgggtgcgc gcgccatcgc catcgtcaag
     1741 gccgacggct acggccacgg cgccgcgcgc ctgctgccgg cactcgccga tgccgacatg
     1801 ctcggcgtcg cctgcatcga ggaggcgctg gcgctgcgcg ccgccggtgc cacgcagccg
     1861 gtgctgctgc tcgaaggcgt gttctcggcc gacgaactgc cggtgtgcgc cgagcaggat
     1921 ttcgagatcg ccatccatga cccgctgcac gtcgaatggc tggagcgcgc ccggctggcc
     1981 acgccgctgc gcgtgtggct gaagctcgac agcggcatgc accgcctcgg cgtgcgtccg
     2041 cagcaggttg cgccgctgct tgcgcgcctg cgcgtctgcg cgaacgtcgc gccgcagctc
     2101 ggcgtcatga gccacctcgc ctgcgccgac gagccggcgc gcgccgagac cgccgcgcag
     2161 ctcgaaacct tcgccgccgc cacggccggc ctgccggtgc gccgggcgct gtcgaactcg
     2221 gccggcgtgc tggcctggcc ggcggcgcat cacgactgga tccggccggg gatcatgctc
     2281 tacggcgcga cgccgatgct cgggcgcacc ggtcccgagg acggcctgaa gccggtcatg
//
LOCUS       DAOQJH010000491         2329 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_34531_length_2329_cov_3.801231, whole genome shotgun sequence.
ACCESSION   DAOQJH010000491 DAOQJH010000000
VERSION     DAOQJH010000491.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2329)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2329)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2329
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34531_length_2329_cov_3.801231"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(44..196)
                     /locus_tag="PLQ18_10760"
     CDS             complement(44..196)
                     /locus_tag="PLQ18_10760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02178.1"
                     /translation="MIRFRPDTKQLCVLALAAALALVSLMVPIDDGKPGPGSEAPAGE
                     HLAHGG"
     gene            257..1075
                     /gene="mazG"
                     /locus_tag="PLQ18_10765"
     CDS             257..1075
                     /gene="mazG"
                     /locus_tag="PLQ18_10765"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HNE02179.1"
                     /translation="MSDVARLLQIMSLLRDPERGCPWDVAQTFASIAPYTLEEAYEVA
                     DAIEREDFAGLKSELGDLLLQVVFHAQMASEQGRFGFADVVATLSDKLVRRHPHVFGD
                     TTYASLKEQGEAWERIKREERAADGEIPAGALDGVPLALPGLTRAVKLTKKAARVGFD
                     WDEPGAVLDKIEEEIRELRAEIHTGAPLARLRDELGDVLFALANLARHLDLDPEAALR
                     GTNAKFERRFRHIEARLAETARTPADATLAEMDGYWNEAKALERGEAPEPSTTR"
     gene            complement(1081..1560)
                     /locus_tag="PLQ18_10770"
     CDS             complement(1081..1560)
                     /locus_tag="PLQ18_10770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007806529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HNE02180.1"
                     /translation="MPCPPRPGNSAAAAIIRACLALAVPWLAAAPATAGQTTQVTVGA
                     GRFRVEIAETEAERERGLMYRAALPAGSGMLFVQPRALPVSFWMKNTHVALDILWFDA
                     QGRLVKISARVPPCTADPCPLYPSGGPVAWVLEIAAGEAARQRIRIGDRLKVEAAKD"
     gene            1559..2164
                     /locus_tag="PLQ18_10775"
     CDS             1559..2164
                     /locus_tag="PLQ18_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020161134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNE02181.1"
                     /translation="MEYRYRIIDRAIAYQGFFTLSEYRLQHERFAGGWSDEISRLCFE
                     RGDAVAVLLWDPRRDAVVLVEQFRVGALGRPYSPWLYEIVAGITGPGETREEVARREA
                     VEEAGCTVEALAFVCEYLCSPGGTSESTALYVGCVDSHGVGGIHGLAHEHEDIRVFVV
                     PRAEAMAMIGGRLNSAAVIIALQWLALNRERLVAEWLPAGG"
BASE COUNT          345 a          782 c          839 g          363 t
ORIGIN      
        1 aaggcgcggc ccggcggggc gatcgacggc gctgaggttg cgctcagccg ccgtgggcga
       61 ggtgctcgcc agccggtgct tcgctgccgg ggccgggctt gccgtcatcg atcggcacca
      121 tcagcgatac gagcgccagg gctgcagcca gggcgagtac acagagctgc ttggtatcgg
      181 ggcggaatcg gatcatggtt ggaagtctcc gcggacgggc gcgtccgcaa tctggaaatt
      241 ttcggcggat actaaggtga gtgacgttgc ccggctgtta caaatcatgt ccctgctgcg
      301 tgatcccgaa cgcggctgcc cgtgggacgt ggcgcagacc ttcgcgagca tcgcgccgta
      361 caccctcgaa gaggcctacg aggtcgccga tgcgatcgag cgggaggact tcgccggact
      421 gaagtccgaa ctgggagacc tgcttttgca ggtggtgttc catgcccaga tggcgagcga
      481 gcaggggcgc ttcggcttcg ccgatgtggt cgcgaccctg tccgacaagc tcgtgcgccg
      541 ccacccgcac gtgttcggcg ataccacgta cgcgagtctc aaagagcagg gcgaggcctg
      601 ggagcgcatc aagcgcgagg agcgcgccgc cgatggcgaa atcccggccg gggcgctcga
      661 tggcgtgccg ctggcgctgc cggggctgac ccgcgcggtc aagctcacga aaaaggccgc
      721 gcgcgtcggc ttcgactggg acgagccggg ggccgtgctc gacaagatcg aggaggaaat
      781 ccgggaactg cgggccgaga tccacaccgg tgcaccgctg gcgcggcttc gcgatgaact
      841 cggcgacgtg ctgttcgcgc tcgcgaacct tgcgcgtcac ctcgatctcg acccggaggc
      901 ggcgctgcgc ggcaccaacg ccaagttcga acgccgcttc cgccacatcg aggcacggct
      961 cgccgaaacc gcgcgcaccc cggccgacgc gacgctcgcc gagatggacg gctactggaa
     1021 cgaggccaag gcactcgaac gcggtgaagc cccggagcca tcgaccacgc gctgaaggct
     1081 tcagtccttc gcggcttcga ccttcagccg gtcgccgatc cggatgcgct gacgcgccgc
     1141 ctcgccggcc gcgatctcca gcacccaggc caccggtccg ccactcgggt acagcgggca
     1201 cgggtcggcc gtgcatggtg gcacgcgcgc actgatcttc acgagccggc cctgcgcatc
     1261 gaaccacagg atgtcgagcg cgacgtgcgt gttcttcatc cagaagctca ccggcagcgc
     1321 ccgcggctgc acgaacagca tgccgctgcc ggccggcagc gcagcgcgat acatcagccc
     1381 gcgctcgcgc tcggcctcgg tttcggcgat ctcgacccgg aagcgtccgg ctccgaccgt
     1441 gacctgcgtc gtctgcccgg ccgttgccgg cgcagcggcg agccacggca cggcgagcgc
     1501 gaggcaggcg cgtatgatcg ccgctgcggc gctgttgccg ggtcggggag ggcagggcat
     1561 ggaataccgg taccggatca tcgatcgggc catcgcctat cagggcttct tcacgctgtc
     1621 cgaataccgg ctgcagcacg agcgcttcgc cggcggctgg agcgacgaga tcagccggct
     1681 gtgcttcgag cgcggcgatg cggtcgccgt gctgctgtgg gatccgcgcc gcgacgccgt
     1741 cgtgctggtc gagcagttcc gcgtcggtgc gctcggccgg ccgtattcgc cgtggctgta
     1801 cgagatcgtc gccggcatca ccggccccgg cgagacgcgc gaggaggtcg cccggcgcga
     1861 ggcggtcgag gaggccggct gcacggtcga ggcgctggcc ttcgtctgcg aatacctctg
     1921 cagccccggc ggcacttcgg agagcacggc gctgtacgtc ggctgcgtcg acagccacgg
     1981 cgtcggcggc atccacggtc ttgcgcacga gcacgaggac atccgcgtgt tcgtcgtgcc
     2041 gcgtgccgag gcaatggcga tgatcggcgg acggctcaat tcggccgccg tgatcatcgc
     2101 gctgcagtgg ctggcgctga accgcgagcg gctcgtcgcc gagtggctgc cggcgggcgg
     2161 ctgagcgccg gcgggccgtg tcaggcgccg ggtctcattc ctcgctcttg cgcgaggcgc
     2221 gcttgcgggc gttctcgtcg agcagcttct tgcggatgcg gatggtcttc ggcgtgacct
     2281 cgaccagctc gtcgtcgtcg atgaattcga gcgcctgttc gagcgtcat
//
LOCUS       DAOQJH010000492         2325 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_34642_length_2325_cov_3.650661, whole genome shotgun sequence.
ACCESSION   DAOQJH010000492 DAOQJH010000000
VERSION     DAOQJH010000492.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2325
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34642_length_2325_cov_3.650661"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            296..1537
                     /locus_tag="PLQ18_10780"
     CDS             296..1537
                     /locus_tag="PLQ18_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014402003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HNE02182.1"
                     /translation="MLQPLSPESRALVDTGRQFFIPNYKPRDMILDRGQGARLWDLDG
                     NEYLDLGGGIAVNCLGHQDPALLAALEAQAKKLWHTSNVYFTEPAIRLAAELVTASGF
                     ASRVFFCNSGAEANEAAIKLARRYATQHLAPSQRRILTFHGSFHGRTLATVTATAQPK
                     YQEGFEPLPGGFVYGPFNDVTGATQLIDAHTCAVLVEPVQGEGGVLPAAPGFLAALRA
                     ACDKVGALLIFDEVQCGIGRTGKLFGHEWDGVRPDALTLAKALGGGMPIGALLAAPKV
                     EEVLGFGTHGSTFGGNPLACAVARAVLARVSDAGFLAGVQRQAERLRAGLHELGMRHD
                     LFREIRGRGLMIGAVLNSRWQGKATDITEFAREDGVLILQAGPDVLRFVPPLTLTDEE
                     LDSALARLATTFARLPAPAQS"
     gene            1544..>2325
                     /locus_tag="PLQ18_10785"
     CDS             1544..>2325
                     /locus_tag="PLQ18_10785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNE02183.1"
                     /translation="MPDADAGALLADLAAAAQPQMLAQVLAWSAINSGSQHLEGLARM
                     AEVLAGAFGRLAPVERLRLAPQQIIGADGVLRERPLGQALRLRVRPEARWQVLLVGHY
                     DTVYGAADAFQTPHWNADGTLNGPGVADLKGGLAVMLAALEAFESGPQALKAALGWTV
                     LLNPDEEIGSPGSAPLLAAEAARHDVGLVFEPSALPDGTLAGARKGSGNFTVTLRGRA
                     AHAGRNPQDGRNAIVAAADCVLGLAALAAAAQGISVNPAKIDG"
BASE COUNT          304 a          888 c          826 g          307 t
ORIGIN      
        1 ccgccggcga gatccgcggc ttcctgacgc agatcccgga accggccagc ctcggcctgc
       61 tggcgatcgg tgccctcggg ctcggacggc tgcgcgcgcg ccggcgctga tcgccgcgcc
      121 cggcccccgg ccagaggccc cggtgatccc ggggcctttt tcgtgggcgt gtgcagctcg
      181 tgcacggcgt ttgccgcggc gcatcacgcg gggcgcgcgc gcgacggcgc atcgctatac
      241 gctaggcagc gggccacccg tcaccggccc ggcgtaaccc gaggagtgct ccgtcatgct
      301 gcaaccgctg tcccccgaaa gccgcgcgct ggtcgatacc ggccggcagt tcttcatccc
      361 gaactacaaa ccgcgcgaca tgatcctcga ccgcggccag ggcgcgcgtc tgtgggacct
      421 cgacggcaac gaatacctcg acctcggcgg cggcatcgcc gtcaactgtc tcggccatca
      481 ggacccggcg ctgctcgccg cgctcgaagc gcaggcgaag aagctctggc acaccagcaa
      541 cgtctacttc accgagccgg cgatccgcct cgccgcggaa ctggtcacgg cctcgggctt
      601 cgcgagccgg gtgttcttct gcaactccgg cgccgaggcc aacgaagcgg cgatcaagct
      661 cgcgcgccgc tacgcgacgc agcatctggc accgtcgcag cgccggatcc tgaccttcca
      721 cggctccttc cacggccgca cgctggcgac cgtcaccgcc accgcgcagc cgaagtacca
      781 ggaaggcttc gaaccgctgc ccggcggctt cgtctacggg ccgttcaacg acgtgaccgg
      841 cgccacgcag ctgatcgacg cccacacctg cgcggtgctg gtcgagccgg tgcagggcga
      901 aggcggcgtg ctgccggccg cgccgggctt cctcgcggcg ctgcgcgcgg cctgcgacaa
      961 ggtcggcgcg ctgctgatct tcgacgaggt gcagtgcggc atcggccgca ccggcaagct
     1021 gttcggccac gagtgggacg gcgtgcgccc cgatgcgctg acgctcgcga aggcgctcgg
     1081 cggcggcatg ccgatcggcg cactgctggc ggcgccgaag gtcgaggaag tcctcggctt
     1141 cggcacgcac ggttcgacct tcggcggcaa cccgctcgcc tgcgcggtgg cgcgggcggt
     1201 gctcgcgcgc gtcagcgacg ccggcttcct cgccggcgtg cagcgccagg ccgagcgcct
     1261 gcgcgccggc ctgcacgaac tcggcatgcg ccacgacctg ttccgcgaga tccgcggccg
     1321 cggcctgatg atcggtgcgg tgctgaacag ccgctggcag ggcaaggcca ccgacatcac
     1381 cgagttcgcg cgcgaggacg gcgtgctgat cctgcaggcc ggccccgacg tgctgcgctt
     1441 cgtgccgccg ctgacgctga ccgacgagga actcgacagc gcgctcgcgc ggctggcgac
     1501 gactttcgcg cgcctgccgg ccccggcgca gagctgagcc ccgatgcctg acgctgacgc
     1561 cggggctctg ctggccgacc ttgccgccgc cgcgcagccg cagatgctcg cgcaggtgct
     1621 ggcctggtcg gcgatcaact ccggcagcca gcacctcgaa ggcctcgcgc gcatggccga
     1681 ggtgctcgcc ggagccttcg gccggctggc gccggtcgag cggctgaggc tcgcgccgca
     1741 gcagatcatc ggcgccgatg gcgtgctgcg cgaacgcccg ctcggtcagg cgctgcgcct
     1801 gcgcgtgcgc ccggaggcgc gctggcaggt gctgctggtc ggccactacg acaccgtcta
     1861 cggtgccgcc gatgccttcc agacgccgca ctggaacgcc gacggcacgc tgaacggccc
     1921 cggcgtcgcc gatctgaagg gcgggctggc ggtgatgctg gccgccctcg aagccttcga
     1981 gtccgggccg caagcgctga aggcggcgct cggctggacc gtgctgctca accccgacga
     2041 ggagatcggc tcgcccggct cggcgccgct gctcgccgcc gaggccgccc gccacgatgt
     2101 cggcctggtg ttcgaaccct cggcgctgcc cgacggcacg ctcgccggcg cgcgcaaggg
     2161 cagcggcaac ttcacggtca cgctgcgcgg gcgcgccgcg cacgccgggc gcaacccgca
     2221 ggacgggcgc aacgccatcg tcgccgccgc cgactgcgtg ctcggcctcg ccgcgctcgc
     2281 cgccgcggcg cagggtatca gcgtcaaccc ggcgaagatc gacgg
//
LOCUS       DAOQJH010000493         2324 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_34669_length_2324_cov_4.424857, whole genome shotgun sequence.
ACCESSION   DAOQJH010000493 DAOQJH010000000
VERSION     DAOQJH010000493.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2324)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2324)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2324
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34669_length_2324_cov_4.424857"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..995
                     /locus_tag="PLQ18_10790"
     CDS             <1..995
                     /locus_tag="PLQ18_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008891845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNE02184.1"
                     /translation="PLIMSAWKLGPALAAGNSVVLKPAEQSPLSALKLAELALEAGLP
                     PGVLNVVTGYGHSAGRALGLHEDVDAVGFTGSTEVGKLFLQYAGQSNLKRVWLECGGK
                     TPHIILADCPDLDAAATAAAWGIFFNQGEMCTAGSRLLVDRRIKDQVLERIVAVGRTL
                     APGDPLDPATQLGAIVSKEQWQRVLDYIDIGRAQGATLALGGAATRGATGGFYIEPTV
                     FDGVNNAMRIAQEEIFGPVLSVLAVDGAEQAVRIANDTMYGLAAAVWTRDLATAHRVS
                     RQLRAGLVWVNCFDADDITTPFGGYRQSGNGRDKSLHAFDKYTELKTIWMRIGD"
     gene            1128..>2324
                     /locus_tag="PLQ18_10795"
     CDS             1128..>2324
                     /locus_tag="PLQ18_10795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005890620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HNE02185.1"
                     /translation="MPIPIRSKQDWQALDAAHHLHPFTDYADLAAKGARIISWAEGAH
                     LHDADGRTILDGMSGLWCVNLGYGRRELAEAAQRQMLELPYYNNFFQTAHPPAIELAH
                     RLAEFAAPDYTRVFFTGSGSESVDTAIRMIWRYWQLQGQPQRNVIIGRRNGYHGSTIA
                     GASLGGMKAVHAQLAHPLPGFVHIDQPYAFGEGAGLDPAAFGLERARQLAQKIEEIGP
                     DKVAAFFAEPIQGAGGVIIPPDTYWPEIERICRHHGILLVTDEVICGFGRTGRRFGAE
                     YFGVKPDLMTVAKGLTSGYVPMGAVLLGARVGAALASGGGEFFHGFTYSGHPVAASVA
                     LEVLRVLERERIIERVREDTGPYLQQRWRTLADHPLVGEARGLGFFGAIELVQDKSSR
                     TRFPEAK"
BASE COUNT          362 a          875 c          750 g          337 t
ORIGIN      
        1 tcccgctgat catgagcgcc tggaagctcg gcccggcgct cgcggccggc aactcggtgg
       61 tgctcaagcc cgccgagcag tcgccgctgt cggcgctgaa gctcgctgaa ctcgcgctcg
      121 aagccgggct gccgccgggc gtgctgaacg tcgtcaccgg ctacggccat agcgccgggc
      181 gcgcgctcgg cctgcacgag gatgtcgacg ccgtcggctt caccggctcg accgaggtcg
      241 gcaagctgtt cctgcagtac gccggccagt cgaacctcaa gcgcgtctgg ctcgaatgcg
      301 gcggcaagac cccgcacatc atcctcgccg actgcccgga cctcgacgcc gccgcgaccg
      361 ccgccgcctg gggcatcttc ttcaaccagg gcgagatgtg cacagccggc tcgcggctgc
      421 tcgtcgatcg ccgcatcaag gaccaggtgc tggagcgcat cgtcgcggtc gggcgcacgc
      481 tcgcccccgg cgatccgctc gatccggcca cgcagctcgg cgccatcgtc agcaaggaac
      541 aatggcagcg cgtgctcgat tacatcgaca tcggccgcgc gcagggcgcg acgctggcgc
      601 tcggcggtgc ggcgacgcgc ggtgcgaccg gcggcttcta catcgagccg accgtgttcg
      661 acggcgtcaa caacgccatg cgcatcgcgc aggaggaaat cttcggcccg gtgctgtcgg
      721 tgctcgccgt cgacggcgcc gaacaggccg tgcgcatcgc caacgacacc atgtacgggc
      781 ttgccgccgc ggtctggacg cgcgatctcg ccaccgcgca ccgggtttcg cgacagctgc
      841 gtgccgggct ggtctgggtc aactgcttcg atgccgatga catcaccacg cccttcggcg
      901 gctaccgcca gtccggcaac ggccgcgaca agtccctgca cgccttcgac aagtacacgg
      961 agctgaagac aatctggatg cgcatcggcg actgagcgcc tgcacgccgc cggcgcggac
     1021 ggaatgcggc acgtccgccg gtcagccacg gagcccatcg cccgccctgt ccctccgcat
     1081 tccctcgcac ccaagcattc cctcgcactc acgaaggtcc cggcaagatg cccatcccga
     1141 tccgcagcaa gcaggactgg caggccctcg acgccgccca tcacctgcac ccgttcaccg
     1201 attacgccga cctggccgcg aaaggcgcgc gcatcatcag ctgggccgaa ggcgcccatc
     1261 tgcatgacgc cgatggccgc accatcctcg acggcatgtc cgggctctgg tgcgtgaacc
     1321 tcggctacgg ccggcgcgag ctcgccgagg ccgcgcagcg ccagatgctg gagctgccgt
     1381 actacaacaa cttcttccag accgcccacc cgccggcgat cgagctcgcg caccggctcg
     1441 ccgagttcgc ggcgcccgat tacacgcgcg tgttcttcac cggctccggc tccgagtcgg
     1501 tcgataccgc gatccgcatg atctggcgct actggcagct gcaggggcag ccgcagcgca
     1561 acgtcatcat cggccggcgc aacggctatc acggctcgac catcgccggc gccagcctcg
     1621 gcggcatgaa ggccgtgcac gcgcagctcg cgcacccgct gccgggcttc gtgcacatcg
     1681 accagcccta cgccttcggc gagggcgcag ggctggaccc ggccgcattc gggctggagc
     1741 gtgcccgcca gctcgcgcag aagatcgagg aaatcggccc ggacaaggtc gcggccttct
     1801 tcgccgaacc gatccagggc gccggcggcg tcatcatccc gcccgacacc tactggccgg
     1861 agatcgaacg catctgccgg caccacggca tcctgctggt caccgacgag gtcatctgcg
     1921 gtttcgggcg caccggccgg cgtttcggcg ccgagtactt cggcgtcaag cccgacctga
     1981 tgacggtcgc caagggcctg acctccggct acgtgccgat gggcgcagtg ctgctcggcg
     2041 cgcgcgtcgg cgcggcactc gccagcggcg gcggcgagtt cttccacggc ttcacctatt
     2101 ccggccaccc ggtcgcggca tcggtcgcgc tcgaagtgct gcgcgtgctc gaacgcgaac
     2161 gcatcatcga gcgcgtgcgc gaggacaccg gcccgtatct gcagcagcgc tggcgcacgc
     2221 tcgccgatca cccgctggtc ggcgaagccc ggggcctggg cttcttcggc gccatcgaac
     2281 tggtgcagga caaatccagc cgcacacgct ttccggaagc caaa
//
LOCUS       DAOQJH010000494         2321 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_34748_length_2321_cov_3.992939, whole genome shotgun sequence.
ACCESSION   DAOQJH010000494 DAOQJH010000000
VERSION     DAOQJH010000494.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2321)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2321)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2321
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34748_length_2321_cov_3.992939"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2037)
                     /locus_tag="PLQ18_10800"
     CDS             complement(<1..2037)
                     /locus_tag="PLQ18_10800"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNE02186.1"
                     /translation="MSEPRNTTARPGGAPRFALYGVALVVSVLLMAGTFFLVQRHETG
                     ITADLALVNEQRLLARTLTALAVEAVQGQDDAFDRLGQARTRFDSSIAALRNGDATTA
                     PVPTEAGDALAQLEKRWQETRGAVSAVLESREPVKAVRALLPVFEETLPALHDYADDL
                     SGGLADGGAPGAQVRAAARLSTLAQRMQYDLGLLVRGDANSALAADRLLRDVSQFGRL
                     AKALREGDPRQGLTPMNAPALLPKLNDITAGAAQLEKSIGQMLAYAPELFQAQDATQR
                     LVNGSAPLAQAAAALGEALTSRADSVHIAGIPVGFGFVYFFAALSLMLLMLLLRKQVV
                     EARVRLEESEQRKQQTEDQNRRNQEAILRLLDEMGDLAEGDLTVQASVTEDITGAIAD
                     SINYAIEALRDLVTTINDTSGRVAAAAEETRSIAERLASASQEQASQIETSGNTIAQM
                     ARSMDEVSSKTASSSEVAQQAAALAREGGERVRRTIQGMDAIREHIQETSKRIKRLGE
                     SSQEIGEITELISDITEQTNVLALNAAIQAASAGEAGRGFSVVAEEVQRLAERSADAT
                     RQISALVKTIQTDTQDAVSAMARSTQGVVDGARLSDAAGAALGDIDRVTRQLADLIER
                     ISAQALQEAESANVVAANIQHIFAVTEQTGEGTRSTAQMVRELSRMAEELRQSVA"
     gene            complement(2119..>2321)
                     /locus_tag="PLQ18_10805"
     CDS             complement(2119..>2321)
                     /locus_tag="PLQ18_10805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HNE02187.1"
                     /translation="SYGLVVDEIIGMRPIGPANRLPELPAGTAPTLKPYARAAYAIDN
                     QVWSEFDIGRLLADPRFMAAAL"
BASE COUNT          288 a          798 c          853 g          382 t
ORIGIN      
        1 cgccaccgac tggcgcagtt cctcggccat gcgggagagc tcgcgcacca tctgggcggt
       61 cgagcgggtg ccttcgccgg tctgctcggt cacggcaaag atgtgctgga tgttggcagc
      121 caccacgttg gccgattcgg cttcctgcag ggcctgcgcc gagatccgtt cgatcaggtc
      181 ggccagctgc cgcgtcacgc ggtcgatgtc gcccagtgcg gcgccggccg cgtcggacag
      241 tcgtgccccg tccaccacgc cctgcgtgga acgtgccatg gcggacaccg catcctgcgt
      301 gtcggtctga atggtcttga ccagggccga gatctgccgc gtggcatcgg cggagcgttc
      361 ggccagccgc tgcacttcct cggccaccac cgagaagccg cgcccggcct cgccggcgct
      421 ggcggcctgg atggcggcgt tcagggccag cacgttggtc tgttcggtaa tgtccgagat
      481 cagctcggtg atctcgccga tctcctgcga ggactcgccg aggcgcttga tgcgcttgga
      541 ggtctcctgg atgtgctcac ggatcgcgtc catgccctgg atcgtgcggc gcacgcgctc
      601 gccgccctcg cgcgccagcg ccgcggcctg ctgcgcgacc tcggaggaac tggccgtctt
      661 cgaggacacc tcgtccatcg agcgcgccat ctgcgcgatg gtgttgccgg aggtctcgat
      721 ctggctcgcc tgttcctgcg aggccgaggc caggcgctcg gcgatcgagc gggtttcctc
      781 ggcagcggcg gcgacgcggc cggaggtgtc gttgatggtc gtgacgagat cgcgcagcgc
      841 ctcgatcgcg tagttgatcg agtcggcgat cgcgccggtg atgtcctcgg tgacgctcgc
      901 ctgcacggtc aggtcgccct cggcgaggtc gcccatctcg tcgagcagcc gcaggatggc
      961 ctcctggttg cggcggttct ggtcttcggt ctgctgcttg cgctgctcgg attcctccag
     1021 gcgcacgcgc gcctcgacga cctgcttgcg cagcagcagc atcagcagca tcagcgacag
     1081 cgccgcgaag aagtacacga agccgaagcc gaccgggatg ccggcgatgt gcacgctgtc
     1141 ggcgcggctc gtgagcgcct cgccgagcgc ggcggcggcc tgcgcgagcg gtgcgctgcc
     1201 gttgaccagc cgctgcgtgg cgtcctgcgc ctggaacagc tcgggcgcgt aggcgagcat
     1261 ctggccgatc gatttttcga gctgcgcggc accggcggtg atgtcgttga gcttcggcag
     1321 cagcgccggg gcgttcatcg gcgtcaggcc ctggcgcgga tcgccttcgc gcagcgcctt
     1381 cgccagccgg ccgaactggc tgacatcgcg cagcagccgg tcggcggcga gcgccgagtt
     1441 ggcatcgccg cgcaccagca gcccgaggtc gtactgcatg cgctgcgcga gcgtcgacag
     1501 ccgggccgcg gcgcgcacct gcgcgcccgg cgcgccgcca tcggcgaggc cgccggagag
     1561 gtcatcggcg tagtcgtgca gggccggcag ggtttcctcg aataccggca gcagcgcgcg
     1621 cacggccttg accggctcgc gcgattcgag cacggcgctg acggcgccgc gcgtctcctg
     1681 ccagcgcttc tcgagctgcg cgagcgcgtc accggcttcg gtcggcaccg gcgccgtggt
     1741 ggcgtcgccg ttgcgcagcg ccgcgatcga gctgtcgaag cgcgtgcgtg cctggccgag
     1801 gcggtcgaag gcgtcgtcct gaccctgcac cgcctcgacc gccagcgccg tcagcgtgcg
     1861 ggccagcagg cgctgttcgt tgaccagggc caggtcggcg gtgatgccgg tctcgtgccg
     1921 ctgcaccaga aagaatgtgc ccgccatcag cagcaccgac acgaccagtg ccacgccgta
     1981 cagcgcgaag cggggtgcgc cgccggggcg cgcggtggtg ttgcggggtt cgctcattgt
     2041 gcttgtgctc cctggtgtaa gcgcccggcg tgtggcgccg ggcgcggcgc tgccggcttc
     2101 cgggctgccg ctggggggtt aaagggccgc cgccatgaat cgcggatccg cgagcaggcg
     2161 accgatgtcg aattcactcc agacctggtt gtcgatcgcg taggccgccc gggcataggg
     2221 cttgagcgtc ggggcggtcc cggccggcag ttccggcagg cggttggccg gaccgatcgg
     2281 gcgcatgccg atgatctcgt cgaccacgag gccgtaggac c
//
LOCUS       DAOQJH010000495         2299 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_35388_length_2299_cov_4.154189, whole genome shotgun sequence.
ACCESSION   DAOQJH010000495 DAOQJH010000000
VERSION     DAOQJH010000495.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2299
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35388_length_2299_cov_4.154189"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="PLQ18_10810"
     CDS             <1..180
                     /locus_tag="PLQ18_10810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HNE02188.1"
                     /translation="GAAPADRPIARGSERPVPVAAPAAAAARPAPATPDPFGERSEQD
                     LEYLDIPAFLRRQAD"
     gene            534..1445
                     /gene="lpxC"
                     /locus_tag="PLQ18_10815"
     CDS             534..1445
                     /gene="lpxC"
                     /locus_tag="PLQ18_10815"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010961647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HNE02189.1"
                     /translation="MIRQRTLKNVIRATGVGLHTGEKVYLTLRPAPPDAGITFRRVDL
                     HAPVEIYAQPENVGDTQLSTTLVRGDVRVSTVEHLLSAFAGLGIDNAVVDVSAPEVPI
                     MDGSAGPFVFLLQSAGIEEQAAPKRFIRIRKTVEVRDGDKWARFRPFDGFKVRFTIDF
                     DHPVFRNRAQQAEIDFSTTSFVKEVSRARTFGFMHEIEYLRERNLALGGSLDNAIVVD
                     EYRILNEDGLRYEDEFVKHKILDAIGDLYLLGHSLIGAFEGYKSGHGLNNALLRALLA
                     DREAWEEVTYEERHAPISFLQPAQVAR"
     gene            1642..>2299
                     /locus_tag="PLQ18_10820"
     CDS             1642..>2299
                     /locus_tag="PLQ18_10820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNE02190.1"
                     /translation="MNLMKLLFGSRNGRAVTRMRAQVAKITALEPAISALDDAGLRAK
                     TDEFRSRLAGGTTLDALLPEAFAVVREAAKRVLGLRPFDVQCIGGMVLHQGKITEMRT
                     GEGKTLTATLPAYLNALEGRGVHIVTVNDYLARRDATQMGRLFGFLGLSTGCILGGMD
                     AAEKRAAYAADITYGTNNEFGFDYLRDNMAFSLEQKVQRAFNFAIVDEVDSILIDEAR
                     T"
BASE COUNT          370 a          837 c          739 g          353 t
ORIGIN      
        1 ggcgcggccc cggccgatcg tccgatcgca cgcggcagcg agcgcccggt gccggtggcg
       61 gccccggccg ctgccgccgc ccgcccggca ccggcgacgc cggacccgtt cggcgagcgt
      121 agcgagcagg atctcgaata cctcgacatc ccggccttcc tgcgccgtca ggccgactga
      181 gggctgcacc cgtttcgcac gcgagcgccc cgcccggcct cgcgcctctg cggctgccgc
      241 acggtaccga ccgtctcccg gtcccgtgcg gcggctttgt cgtttccggg ccgctccggg
      301 ccactccggc cacgtagtga agtcgcaaca gccgtgaacc accggtgaca agcgtgtgac
      361 caaaccacca cgcatggcac ttgattgttg tttttgtgcc ccgcacaatg acacatgctg
      421 ttgcgcagtt catactaagc gccagtcagt catccaaccg tccggtccgc aacgtacgcc
      481 cctcgtcggt gccggcccgg tccccaaccg gctgtgacat gtcggggtat cccatgatca
      541 ggcagcgcac gctcaagaac gtcatccgcg ccaccggcgt cggcctgcac accggcgaga
      601 aggtctatct gacgctgcgc ccggcgccgc cggatgccgg catcaccttc cgccgcgtcg
      661 acctgcacgc gccggtcgag atctacgcgc agcccgagaa tgtcggcgac acccagctgt
      721 cgacgacgct ggtgcgtggc gacgtgcgcg tctcgacggt cgagcacctg ctgtcggcct
      781 tcgccggcct cggcatcgac aatgccgtcg tcgacgtgag cgcgccggaa gtgccgatca
      841 tggatggcag cgccggtccg ttcgtgttcc tgctgcagtc ggcgggaatc gaggagcagg
      901 ccgcaccgaa gcgcttcatc cgcatccgca agaccgtcga ggtgcgtgac ggcgacaagt
      961 gggcgcgctt ccggccgttc gacggcttca aggtcaggtt cacgatcgac ttcgatcacc
     1021 cggtgttccg caaccgtgcg cagcaggccg agatcgactt ctcgaccact tccttcgtca
     1081 aggaagtctc gcgcgcccgc accttcggct tcatgcacga gatcgaatac ctgcgggagc
     1141 gcaacctcgc gctcggcggc agcctcgaca acgccatcgt cgtcgacgag taccgcatcc
     1201 tcaacgagga tggcctgcgc tacgaggacg agttcgtcaa gcacaagatc ctcgacgcga
     1261 tcggggatct ctacctgctc ggccacagcc tgatcggcgc cttcgagggc tacaagtccg
     1321 gtcacggcct caacaacgcg ctgctgcgcg cgctgctcgc cgaccgcgag gcctgggaag
     1381 aagtcaccta cgaggagcgc cacgcgccga tctcgttcct gcagccggcg caggtcgccc
     1441 gctgagccga agcgcgtagc cagctgtctg cgcctgcacg ggcgccgttc gcgcagagcg
     1501 cggacggcgc ccgttttgca tccggccggc ctgcaccacc gtaatgcagc ccggccgggg
     1561 ccgggcacgc aggtgcgcgg catcgggtat catccggcgc ctcgccgcgt gcaggcgcgc
     1621 gtttcagcca aaggctcagt catgaatctg atgaagctgc tgttcggcag ccgcaacggt
     1681 cgcgcggtca cccgcatgcg cgcccaggtc gccaaaatca ccgcgctgga gccggcgatc
     1741 tcggcgctcg acgatgccgg cctgcgggcg aaaaccgatg aattccgctc gcggctggcg
     1801 ggcggcacga cgctcgacgc gctgctgccc gaagccttcg ccgtcgtgcg cgaggccgcg
     1861 aagcgcgtgc tcggcctgcg gccgttcgac gtgcagtgca tcggcggcat ggtgctgcat
     1921 cagggcaaga tcaccgagat gcgcaccggc gagggcaaga ccctgaccgc gacgctgccg
     1981 gcctacctga acgccctcga aggcaggggc gtgcacatcg tcaccgtcaa cgactacctc
     2041 gcgcgccgcg acgcgacgca gatgggacgg ctgttcggct tcctcggcct cagcaccggc
     2101 tgcatcctcg gcggcatgga cgcggccgag aagcgcgccg cctacgccgc cgacatcacc
     2161 tacggcacca acaacgagtt cggcttcgac tacctgcgcg acaacatggc gttctcgctg
     2221 gagcagaagg tgcagcgcgc cttcaacttc gcgatcgtcg acgaggtcga ctcgatcctg
     2281 atcgacgagg cgcgcacgc
//
LOCUS       DAOQJH010000496         2299 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_35399_length_2299_cov_2.992870, whole genome shotgun sequence.
ACCESSION   DAOQJH010000496 DAOQJH010000000
VERSION     DAOQJH010000496.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2299
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35399_length_2299_cov_2.992870"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..732
                     /locus_tag="PLQ18_10825"
     CDS             <1..732
                     /locus_tag="PLQ18_10825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017354788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HNE02191.1"
                     /translation="GMAKNTYGTGCFLLMHTGAQAVCSRHGLLTTIAWQIGDQLEYAL
                     EGSIFVAGSAVQWLRDGLRLIDTAADSGALAQSVASTGGVYCVPAFVGLGAPYWRSDV
                     RGALFGLTRGSGRAEICRAVLEAIAFQTRDVLDAMQADAGLALERLRVDGGAVANAFL
                     MQFQSDILGVPVERPAQPEATAFGAAALAGLATGFWADRAAIAACCRPDRRFEPALPA
                     AGREASYAGWRQAVQATLGFRIDPA"
     gene            complement(748..1674)
                     /locus_tag="PLQ18_10830"
     CDS             complement(748..1674)
                     /locus_tag="PLQ18_10830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family ion channel"
                     /protein_id="HNE02192.1"
                     /translation="MVILQTPGGFDLLFRIRGSIVRDILRPLLATTALATLVTVLHES
                     VLPFRYSMTVTPFSLIGLTLAIFLGFRNSVSYDRYWEGRKLWGELLNACRSLARLALT
                     TIDAPAGAADARPTAFVHRLMAFAHALRHHLRGSDPLPDLERLLGPQAAARPAAAHNV
                     PLALLQELGEHLHGARRDLRIDPLLAAHAESRLDRLADVLGGCERIRNTPLPYPYRLL
                     LHRTVYFYCLLLPFGLIDTVGRMTPVVVLFLAYTFFGLDALGEQIEEPFGEMPHDLPL
                     LAISTTIEADLLELLGERERPAAVRPVDGVLL"
     gene            complement(1748..1828)
                     /locus_tag="PLQ18_10835"
     tRNA            complement(1748..1828)
                     /locus_tag="PLQ18_10835"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1788..1790),aa:Arg,seq:ccg)
     gene            1926..>2299
                     /locus_tag="PLQ18_10840"
     CDS             1926..>2299
                     /locus_tag="PLQ18_10840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HNE02193.1"
                     /translation="MANPIDTRAKRERLPHRSDPYWARIEKGAYLGFRTSEKTEGTWL
                     ARWRDADGKQQYRPLGTQPNYDAAAALARAWFAQCTGGGAVEVPTVEQVCVAYVEALA
                     SEGRKATAADARGRFARLVCGQP"
BASE COUNT          361 a          765 c          844 g          329 t
ORIGIN      
        1 ggcatggcca agaacaccta cggcaccggc tgcttcctgc tgatgcacac cggcgcgcag
       61 gcggtgtgtt cacgccacgg gctgctgacg acgatcgcgt ggcagatcgg cgatcagctc
      121 gaatacgcgc tcgaaggcag catcttcgtc gccggctcgg ccgtgcagtg gctgcgcgac
      181 ggcctgaggc tgatcgatac cgccgccgat tccggggcgc tggcgcagtc ggtcgcctcg
      241 accggcggcg tctactgcgt gccggccttc gtcgggctcg gtgcgccgta ctggcgaagc
      301 gacgtgcgcg gtgcgctgtt cgggctgacg cgcggcagcg gccgggccga gatctgccgt
      361 gccgtgctcg aagcgatcgc cttccagacc cgcgacgtgc tcgatgcgat gcaggctgat
      421 gcggggcttg cgctcgaacg gctgcgcgtc gacggcggcg cggtcgccaa cgccttcctg
      481 atgcagttcc agagcgacat cctcggcgtg ccggtcgaac gcccggccca gcccgaagcc
      541 acggccttcg gcgccgcggc gctcgccggg ctggcgaccg gcttctgggc cgatcgcgcc
      601 gccatcgccg cctgctgccg gcccgatcgt cgcttcgagc cggcgctgcc ggctgccggg
      661 cgcgaggcga gctacgcggg ctggcgacag gcggtgcagg cgacgctggg gttcaggatc
      721 gatccggcct gagggcgcgg cccggtctca cagcagcacg ccatcgaccg gccgcacggc
      781 cgccggccgt tcgcgctcgc cgagcagttc gagcaggtcg gcctcgatcg tcgtgctgat
      841 cgccaagagc ggcagatcgt gcggcatctc gccgaacggt tcctcgatct gctcgccgag
      901 ggcatcgagg ccgaagaacg tgtaggcgag gaacagcacg accaccggcg tcatccggcc
      961 gaccgtgtcg atcaggccga agggcagcag caggcagtaa aagtacacgg tacggtgcag
     1021 cagcagccgg tacgggtacg gcagcggcgt gttgcggatg cgttcgcagc cgccgagcac
     1081 gtcggcgagc cggtcgagcc gggattcggc atgcgcggcg agcagcgggt cgatgcgcag
     1141 gtcacggcgc gcgccgtgca ggtgctcgcc gagttcctgc agcagcgcca gcggcacgtt
     1201 gtgcgccgcc gccggacggg cggcggcctg cgggccgagg aggcgttcga ggtcggggag
     1261 cggatcactg ccgcgcaggt ggtggcgcag cgcatgcgca aacgccatca gccggtgcac
     1321 gaacgccgtc ggccgcgcat cggcggcgcc ggccggggca tcgatcgtcg tcagcgccag
     1381 tcgggccagg ctgcggcagg cgttcagcag ttcgccccag agcttgcggc cttcccagta
     1441 gcggtcgtag ctgacgctgt tgcggaagcc gagaaagatc gcgagggtca ggccgatcag
     1501 gctgaacggc gtgacggtca tcgaataacg aaacggcagc accgactcgt gcagcaccgt
     1561 gaccagcgtc gcgagcgccg tcgtcgccag cagcggacgc aggatgtccc gcacgatcga
     1621 gccgcggatg cggaacagca gatcgaaacc gccgggcgtc tgcaggatga ccatggcgcg
     1681 tgaccggagc cgcgggtgaa tcggtgacgt cggggagcgc agacagcggg aacggcgggg
     1741 tgaggactgg cgcgcccgaa gggattcgaa cccctgacct ctgcctccgg agggcaggga
     1801 gaagggtccg gagccgtccg cagcgcgcgg caagtacatt gattttactg aaatgttatc
     1861 catagctgtc cgtgaaagtc cgtgctatct ttttcatatt cttttcatga gcgcggattc
     1921 acgacatggc caaccccatc gacacccgtg ccaagcgcga gcgtctgccc catcgaagcg
     1981 atccctattg ggcgcgcatc gagaagggcg cgtatctggg tttccgcacc agcgaaaaga
     2041 ccgaagggac ctggctcgcc cgctggcgcg acgccgacgg caagcagcag taccgcccgc
     2101 ttgggacgca gcccaactat gacgccgccg ccgctctggc ccgcgcatgg tttgcccaat
     2161 gcaccggggg cggcgccgtc gaggtgccga cggtcgaaca ggtttgcgtc gcctacgtcg
     2221 aggcgctggc cagcgagggg cgcaaggcga cggccgccga tgcccgcggc cggttcgccc
     2281 ggctggtctg cgggcagcc
//
LOCUS       DAOQJH010000497         2297 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_35454_length_2297_cov_3.141838, whole genome shotgun sequence.
ACCESSION   DAOQJH010000497 DAOQJH010000000
VERSION     DAOQJH010000497.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2297)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2297)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2297
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35454_length_2297_cov_3.141838"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..941)
                     /locus_tag="PLQ18_10845"
     CDS             complement(<1..941)
                     /locus_tag="PLQ18_10845"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009044329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNE02194.1"
                     /translation="MDIEIPGYDIEGELGAGAMAAVYLATQRSLDRRVALKVMAPTLA
                     VDEEFCKRFLREGKTLAKLQHPHIVAIYDIGNDGSRYYMAMEYVDGGTLKERIGREGL
                     PPEQALAVMREIAGALGYAHARGLVHRDVKPANILYRADGTAVLSDFGIAKTFDDGTQ
                     LTSAGFTVGTPNYMSPEQARGAHLDGRSDLYSLGVMFYELLTGALPYRGTDSFSTALM
                     HLSAPIPMLPPALARFQPLLDRMLAKAPEDRFPNAAALLAFDIDAAWAERQQQEQGGT
                     VALTAVPQAAAGSAATTTGSAAGATAIGGGTQLADAGS"
     gene            1133..1738
                     /locus_tag="PLQ18_10850"
     CDS             1133..1738
                     /locus_tag="PLQ18_10850"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNE02195.1"
                     /translation="MKNFLLNAPRVALLAVALAGCSTFSSSGGSGSSTSAPAAEVKPE
                     IAEQLKALRAEADKGNPAAQTRLGLVYARGEAGVPADLTQATQWFRKASDQGFPPAQT
                     NLAVAYLTGQGGVTQDPAEAARLTRAAADKGWPPAMTQLGFMYGKGVGVRQDWKEAER
                     WLKLAADKNDDNAKRLLAELKQMRAQQGGGAPAPAAPPKKK"
     gene            complement(1754..1990)
                     /locus_tag="PLQ18_10855"
     CDS             complement(1754..1990)
                     /locus_tag="PLQ18_10855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02196.1"
                     /translation="MTATVQETGTETTTARLSLELDADTLARAQAAAAAAGLPLDVWL
                     QGFVRDRLAEACPAPDTAWRDLPEPGAGPAGGAG"
     gene            complement(2003..>2297)
                     /locus_tag="PLQ18_10860"
     CDS             complement(2003..>2297)
                     /locus_tag="PLQ18_10860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006757979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HNE02197.1"
                     /translation="DITRVVECNCSLCRRRGHLLWFVARSDVRLLTPEADIATYTFGS
                     ATVRHQFCAHCGCGTFGAATDPAGVERLAINVRCLDGVDLDALEVAHYDGASV"
BASE COUNT          331 a          812 c          833 g          321 t
ORIGIN      
        1 gagccggcat cagcgagctg cgtgccgccg ccgatggcgg tcgcgccggc ggcgctgccg
       61 gtcgtcgtcg ctgcagatcc ggccgcagcc tgcggcacgg cagtgagcgc gacggtgccg
      121 ccctgctcct gctgctggcg ttcggcccag gcggcatcga tatcgaaggc gagcagcgcc
      181 gcggcgttcg ggaagcggtc ctcgggcgcc ttggcgagca tccgatcgag cagcggctgg
      241 aagcgcgcga gcgccggcgg cagcatcggg atcggcgcgc tcaggtgcat cagcgccgtc
      301 gagaagctgt cggtgccgcg atacggcagc gcgccggtca gcagctcgta gaacatcacg
      361 ccgagcgaat agaggtcgga gcggccgtcg aggtgggcgc cgcgcgcctg ctcgggactc
      421 atgtagttcg gcgtgccgac cgtgaagccg gccgaggtga gctgcgtgcc gtcgtcgaag
      481 gtcttggcga tgccgaagtc cgacagcacg gcggtgccgt cggcgcggta caggatgttg
      541 gcgggcttca cgtcgcggtg cacgaggccg cgcgcatggg cgtagccgag cgccccggcg
      601 atctcgcgca tgacggccag cgcctgctcg ggtggcaggc cttcgcggcc gatgcgctcc
      661 ttcagcgtgc cgccgtcgac gtactccatc gccatgtagt agcggctgcc atcgttgccg
      721 atgtcgtaga tcgcgacgat gtgcggatgc tgcagcttcg cgagcgtttt gccctcgcgc
      781 aggaagcgct tgcagaactc ttcgtcgacc gcgagggtcg gtgccatgac cttcagcgcg
      841 acacgccggt ccagcgagcg ctgggtggcg agatagacgg cggccatcgc gccggcgccg
      901 agctcgccct cgatgtcata gcccggaatt tcgatgtcca tccggtttgc tgccccccgg
      961 cctgcacagg gccgcagatt tgcggcgagg ccggattatc ggtgccgtaa ccgcagcacg
     1021 ccagccggca tgccggcgca tttgcccgga cggcgacggt gcggataatg ccgccaccct
     1081 tgcgcccggc gacttcgccg gtcgcgctca cgtcctcccg aggagattcc ccttgaagaa
     1141 cttcctgctg aacgccccgc gcgtcgccct gctcgccgtc gccctcgccg gctgcagcac
     1201 gttctcgtcg tccggcggtt cgggcagcag cacgtcggcc ccggccgccg aggtcaagcc
     1261 cgagatcgcc gaacagctca aggcgctgcg cgccgaggcc gacaagggca atccggccgc
     1321 gcagacgcgg ctcggtctgg tctatgcgcg cggcgaggcc ggcgtgccgg ccgatctgac
     1381 gcaggccacg cagtggttcc gcaaggcgtc cgaccagggc ttcccgccgg cgcagaccaa
     1441 tctcgccgtc gcctacctga ccggtcaggg cggcgtcacg caggacccgg ccgaagccgc
     1501 acggctgacg cgcgccgccg ccgacaaggg ctggccgccg gcgatgacgc agctcggctt
     1561 catgtacggc aagggcgtcg gcgtgcgtca ggactggaag gaagccgaac gctggctgaa
     1621 gctcgccgcc gacaagaacg acgacaacgc caagcgcctg ctcgccgaac tgaagcagat
     1681 gcgcgcgcag cagggcggcg gcgcaccggc gccggcagcc ccgccgaaga agaagtaagc
     1741 ccgccccctg ccctcagccg gccccgcccg cggggccggc tccgggctcc ggcaggtcgc
     1801 gccaggcggt atccggcgcc ggacaggctt cggccagccg gtcgcggacg aagccctgca
     1861 gccacacgtc cagcggcagc ccggccgctg cagcggcggc ctgcgcgcgc gccagcgtgt
     1921 cggcatcgag ttcgaggctc aggcgtgccg tcgtcgtttc cgtgcccgtt tcctgcaccg
     1981 ttgccgtcat cgcgattgct cctcagaccg aggcgccgtc gtagtgcgcg acttcgaggg
     2041 catcgaggtc gacgccgtcc agacagcgca cgttgatcgc caggcgctcg acgccggccg
     2101 gatcggtggc cgcgccgaag gtgccgcagc cgcagtgcgc gcagaactgg tggcgcacgg
     2161 tcgcggagcc gaaggtgtag gtggcaatgt cggcttccgg cgtcagcagc cgcacgtcgc
     2221 tgcgcgcgac gaaccacagc aggtggccgc gccggcggca cagcgagcag ttgcattcga
     2281 cgacgcgcgt gatgtcg
//
LOCUS       DAOQJH010000498         2295 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_35510_length_2295_cov_3.066518, whole genome shotgun sequence.
ACCESSION   DAOQJH010000498 DAOQJH010000000
VERSION     DAOQJH010000498.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2295)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2295)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2295
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35510_length_2295_cov_3.066518"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..762)
                     /locus_tag="PLQ18_10865"
     CDS             complement(<1..762)
                     /locus_tag="PLQ18_10865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02198.1"
                     /translation="MTTVFQPVVPAAPEGPQAPLRATPIDARLSRTYYFDGRLLTAAD
                     LIRDQQYLDRRLLDVGRGLGDGIVEGLALALSGSTLSVQPGVAVSADGRTLQLDAPLL
                     VDLDDRATLAALNTGADSDGVLHSGRFTNGLYAVALGYAEQGSGSAEVFPRDFGAARG
                     LNFDAIDEGVELRLVPLTVPAPPLPALAARALLARTLVGAGRLDHWLPADAVALGVLA
                     IDGDAVLWCDPWLLRHPRRPDAVLDGFQEDLLAHYR"
     gene            complement(776..1594)
                     /locus_tag="PLQ18_10870"
     CDS             complement(776..1594)
                     /locus_tag="PLQ18_10870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02199.1"
                     /translation="MTDADQDSPLRHDAEAGPLARVAFRPGMLLGVEALLAEQDYHRR
                     RLNRHARLLHGLGTIYGLLVTLEPPAAPLPLRLVVHPGIALDALGRELLLPEPYGIDL
                     PAWLAEQGAEALLPFSTPPDAAEGAPRSLVLEVALRYVDCALGLQPVLAAATNASTDA
                     VAASRAGEAFELLLRGADLNPPPPDPPPATLPSPAEQARLDAAADEAERARLQGRLDL
                     LHRTRGATTLPLLGPGDASLVLARVTLTLTTLSAPQPAAADVAVDNLIREFAAS"
     gene            complement(1668..>2295)
                     /locus_tag="PLQ18_10875"
     CDS             complement(1668..>2295)
                     /locus_tag="PLQ18_10875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02200.1"
                     /translation="AHLGELLAWRGTLRGVVLALDLATDGAVARGEIVPVENFRLRRS
                     MATLLGLLPDADAHPLTLGTTRSGNSIVGDSLILADSLDLDRPETRAFLALFAPEQAR
                     AGDERAAVRVFFERYAHRLSVLLHGPGRAWQGVVEQVLEDELPAHLARDVYPAEFPFV
                     PGLSPLLGYDTWLETTPPPAGVQLDFTRLGRAGVLLDAASLLPAGAPS"
BASE COUNT          319 a          805 c          906 g          265 t
ORIGIN      
        1 gcggtagtgc gcgagcaggt cttcttgaaa gccgtcgagc acggcatcgg gccggcgcgg
       61 gtggcgcagc agccaggggt cgcaccagag cacggcatcg ccgtcgatcg cgagcacgcc
      121 gagcgcgacg gcatccgcgg gcagccagtg atcgagccgg ccggcgccga ccagcgtgcg
      181 cgcgagcagc gcgcgcgccg cgagcgccgg cagcggcggg gccggcacgg tcagcggcac
      241 caggcgcagt tccacgcctt cgtcgatcgc gtcgaaattc aggccgcgcg cggcgccgaa
      301 gtcgcgcggg aacacctcgg cgctgccgct gccctgttcg gcgtagccga gcgcgaccgc
      361 gtacaggccg ttcgtgaaac gcccggagtg cagcacgcca tcgctgtctg cgccggtgtt
      421 cagggccgcg agcgtggcgc ggtcgtcgag atcgacgagc agcggtgcat cgagctgcag
      481 cgtgcggccg tcggcgctga ccgcgacgcc gggctgcacg ctcagcgtgc tgccggacag
      541 cgcgagcgcg agcccctcga cgatgccgtc gccgagcccg cgcccgacat cgagcaggcg
      601 gcggtcgagg tactgctggt cgcggatcag gtcggcagcg gtcagcagcc ggccgtcgaa
      661 gtaataggtg cggctcaggc gcgcatcgat cggcgtggcg cgcagcggcg cctgcgggcc
      721 ctcgggcgcg gccggcacga ccggctggaa cacggtggtc atcgcgaagc ctccgtcacg
      781 aagccgcgaa ctcgcggatc aggttgtcga cggccacgtc ggccgcggcc ggctgcggcg
      841 cggacagcgt cgtcagcgtc agcgtcacgc gcgcgagcac gaggctcgcg tccccggggc
      901 cgagcagcgg cagcgtcgtc gcgccgcgcg tgcggtgcag cagatcgagc cgaccctgca
      961 gccgcgcgcg ttcggcctcg tcggcggcgg cgtcgagccg ggcctgctcg gcggggctcg
     1021 gcagcgtcgc cggcggcggg tcgggcggcg gcgggttcag gtcggcaccg cgcagcagca
     1081 gctcgaaggc ctcgccggcg cggctcgcgg cgactgcgtc ggtcgaggcg ttggtcgcgg
     1141 ccgccagcac cggctgcagg ccgagcgcgc agtcgacgta gcgcagagcg acttcgagca
     1201 cgagcgagcg cggcgcgcct tcggcggcat cgggcggcgt gctgaatggc agcagtgcct
     1261 cggcgccctg ctcggcgagc caggcgggca ggtcgatgcc gtagggctcg ggcagcagca
     1321 gttcgcgccc gagcgcatcg agcgcgatgc ccggatgcac gaccagccgc agcggcagcg
     1381 gtgcggccgg cggctccagc gtcaccagca ggccgtagat cgtgcccaag ccgtgcagca
     1441 gccgcgcatg gcggttcagc cgccggcggt gatagtcctg ctcggcgagc agcgcctcga
     1501 cgccgagcag catgccgggg cggaacgcga cgcgcgcgag cggtccggct tcggcgtcat
     1561 ggcgcagcgg agagtcctga tcggcatcgg tcatggccgc gctccagcgg gcagcattgc
     1621 cacggcgtcg tggcgcagcg gagagtcgaa atcggtagcg gtcccggtca tgacggcgct
     1681 ccagcgggca gcagcgacgc ggcatcgagc agcacgccgg cgcggccgag gcgggtgaag
     1741 tcgagctgca cgccggccgg cggcggcgtg gtttccagcc aggtgtcgta gccgagcagc
     1801 ggcgacagcc cgggcacgaa cgggaattcg gccgggtata cgtcccgggc cagatgcgcc
     1861 ggcagttcgt cttcgagcac ctgctcgacc acgccctgcc aggcgcggcc ggggccgtgc
     1921 agcagcacgc tcaggcggtg cgcgtagcgt tcgaagaaca cccgcaccgc ggcgcgctcg
     1981 tcgccggccc gtgcctgttc cggcgcgaac agcgccagaa acgcgcgggt ttcgggccgg
     2041 tcgaggtcga ggctgtcggc gaggatcagg ctgtcgccga cgatcgagtt gcccgagcgc
     2101 gtcgtgccga gcgtcagcgg atgcgcgtcg gcatcgggca gcaggccgag cagcgtcgcc
     2161 atgctgcggc gcagccggaa gttctcgacc ggcacgatct cgccgcgggc cacggcgcca
     2221 tcggtcgcga gatcgagcgc cagcacgacg ccgcgcagcg tgccgcgcca cgcgagcagc
     2281 tcgcccagat gcgcg
//
LOCUS       DAOQJH010000499         2292 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_35587_length_2292_cov_3.238266, whole genome shotgun sequence.
ACCESSION   DAOQJH010000499 DAOQJH010000000
VERSION     DAOQJH010000499.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2292
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35587_length_2292_cov_3.238266"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1183
                     /locus_tag="PLQ18_10880"
     CDS             <1..1183
                     /locus_tag="PLQ18_10880"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing phosphodiesterase"
                     /protein_id="HNE02201.1"
                     /translation="ALAERLNALAARPACLLARIAGDEFALAATGLPDEQAAGRFVAG
                     LHTRLTEPLPVLPEPVSVSVDIGYALAPRDGRDGETLLRHAVAAARQARVSGEGWFGF
                     DPQLGQRMQRRALLERSLRTAISGGELSLVYQPQIALADERVIGVEALMRWNHPRLGA
                     VSPAEFIPIAEETGLIRTLGAWALDTACHEAAGWQRHCPRPPRVAVNLSARQFTDPGL
                     ITGITHALHSSGLPPAQLEIEITESLLMDNPARTVGVLMALRRTGVGVAIDDFGTGYS
                     SLSYLKTFPLDRLKIDRAFVRDLPDNSDSAAIVQAIIALAHTLGLSVIAEGVEDEAQL
                     EFLLGQDCDEVQGYLFAPPRAPAELHALLARGTTHPPSAGRRQAQSKGCGETVSNEPR
                     A"
     gene            complement(1135..2034)
                     /locus_tag="PLQ18_10885"
     CDS             complement(1135..2034)
                     /locus_tag="PLQ18_10885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HNE02202.1"
                     /translation="MNLPAADSPVIRHPRRVEGIVAGQATSDGAGVRLVRLLGAELQR
                     RLDPWLMVDAFSSDRPDDYLAGFPSHPHRGFETVTVMHAGRMRHRDSAGHEGLLRGGD
                     VQWMIAGRGLVHSEMPEQEDGLMAGYQLWLNLPAADKLRAPAWRDIPAAAIPQVRTAA
                     GVQVRVIAGASHGVEGAVQRPHTAPLLLDLSLPAGSRFAQPLPAAHHGFVIVRRGQLA
                     IGSRTVTAGRLALLAQEADSDGVVLDAADQDVEALLVAGRPLGEPIVQHGPFVMNTRA
                     EILAAFEDYARGSFDTVSPQPFD"
BASE COUNT          313 a          860 c          821 g          298 t
ORIGIN      
        1 ggcgctggcc gagcgcctga acgccctcgc cgcccgcccg gcctgcctgc tcgcacgcat
       61 cgccggcgac gagttcgcgc tggcagcgac cgggctgccc gacgagcagg ccgccgggcg
      121 cttcgtcgcc ggcctgcaca cgcgcctcac cgaaccgctg ccggtgctgc ccgagccggt
      181 cagcgtcagc gtcgacatcg gctatgcgct ggccccgcgc gatggtcgcg acggcgagac
      241 gctgctgcgc cacgcggtcg cggccgcccg ccaggcgcgc gtttccggcg agggctggtt
      301 cggcttcgat ccgcaactcg gccagcgcat gcagcgtcgc gcgctgctcg aacgctcgct
      361 gcgcacagcg atcagcggcg gcgagctgtc cctggtctac cagccgcaga tcgcgctggc
      421 cgatgagcgc gtgatcggcg tcgaggccct gatgcgctgg aatcatccgc gcctcggcgc
      481 cgtgtcaccg gccgagttca tcccgatcgc cgaggagacc ggcctgatcc gcacgctcgg
      541 agcctgggcg ctcgacacgg cctgccacga ggccgccggc tggcagcggc actgcccccg
      601 gccgccgcgc gtggccgtca acctgtcggc gcgccagttc accgatcccg ggctgatcac
      661 ggggatcacg catgcgctgc actcgagcgg cctgccgccg gcgcagctgg agatcgagat
      721 caccgaatcg ctgctgatgg acaacccggc acgcaccgtc ggggtgctga tggcgctgcg
      781 ccgcaccggc gtcggcgtgg cgatcgatga cttcggcacc ggctattcgt cgctctccta
      841 cctgaagacc ttcccgctcg accggctgaa gatcgaccgg gccttcgtgc gcgacctgcc
      901 cgacaactcg gattcggcgg cgatcgtgca ggcgatcatc gccctcgccc acacgctcgg
      961 cctcagcgtc atcgccgagg gcgtcgaaga cgaggcccag ctcgaattcc tgctcggcca
     1021 ggactgcgac gaggtgcagg gctatctgtt cgcgccaccg cgggcaccgg cggaactgca
     1081 cgccctgctc gcccgcggca cgacgcaccc gccctccgcc gggcgccggc aggctcagtc
     1141 gaaaggctgc ggcgaaaccg tgtcgaacga gccgcgcgcg tagtcctcga aggccgcgag
     1201 gatctcggcg cgcgtgttca tcacgaacgg cccgtgctgc acgatcggct ccccgagcgg
     1261 gcggccggcg accagcagcg cctcgacgtc ctgatccgct gcgtccagca cgacgccgtc
     1321 gctgtcggcc tcctgcgcga gcagcgccag acggccggcc gtgaccgtgc gggagccgat
     1381 cgccagctga ccgcggcgca cgatgacgaa gccgtggtgc gccgccggca gcggctgcgc
     1441 gaagcggctg ccggccggca ggctcaggtc gagcagcagc ggtgcggtgt gcggccgctg
     1501 cacggcgccc tcgaccccgt ggctcgcacc ggcgatcacg cgcacctgca cgccggcggc
     1561 ggtgcgcacc tgcggaatcg ccgccgccgg gatgtcgcgc caggccgggg cgcgcagctt
     1621 gtcggccgcc ggcaggttca gccagagctg gtagccggcc atcagcccgt cctcctgctc
     1681 cggcatctcc gaatgcacga ggccgcgccc ggcgatcatc cactgcacgt cgccgccgcg
     1741 cagcaggccc tcgtgaccgg cactgtcgcg gtggcgcatg cgcccggcgt gcatgacggt
     1801 gacggtctcg aagccgcggt gcggatgcga cgggaagccg gccaggtaat cgtccgggcg
     1861 gtcgctggaa aaggcatcga ccatcagcca gggatcgagc cgccgctgca gctcggcccc
     1921 gagcaggcgc acgaggcgca caccggcgcc gtccgaagtc gcctgaccgg cgacgatgcc
     1981 ctcgacgcgg cgcggatgac ggatgacggg ggaatcggca gcaggcaggt tcatggcgag
     2041 gcctccgggc gggggttctg ggggtcgggg attgcgcagc accggggtgg gcgcaccggg
     2101 cagcacgccg gacgatcgtg ctgcccgcat gcaggccgct ccagggcccg ccgggtgccg
     2161 gcttcaggcg gcgacggcat ccgcagcttc gtcaaacagc gccgcgagct gtcgcgccgc
     2221 atcggcaaga ccggccgcgc gtgcttcgtc gcccatcgcg aagccttcgg cgtagacgtg
     2281 gcgtacatcg cc
//
LOCUS       DAOQJH010000500         2284 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_35810_length_2284_cov_4.616869, whole genome shotgun sequence.
ACCESSION   DAOQJH010000500 DAOQJH010000000
VERSION     DAOQJH010000500.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2284)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2284)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2284
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35810_length_2284_cov_4.616869"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1209
                     /locus_tag="PLQ18_10890"
     CDS             <1..1209
                     /locus_tag="PLQ18_10890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HNE02203.1"
                     /translation="RCYLVAGCYATANFGRRSLTQGTRSLLNWLVAGRNLGYSEWRVH
                     DLPPATIENAWQALADAAAPQLSLADLPSAAATPLARDALAADSLAALAPPGRLPPGW
                     QLASFSSLQHGAGHEAAACDHDGRGERSPMSAARSEDSAGELAADDILHFPRGAAAGD
                     CLHALFEAIDFTDPDGWPDAIARVLAAHPQALPGLPATEADRRLARMLRRLLDDVLAT
                     TLPGGIVLGALGRRGRLVELGFHLSTGEITPKVLNGWLQKHGYAMPRLGFPPLAGYLK
                     GFIDLVFCHADRYYVLDWKSNHLGYTSADYDCAPIAAAMAEHGYHLQYLIYTVALHRY
                     LRRRIPDYDYERDFGGVLYLFVRGVRPAWRMVAADGESIACGIYHHRPSAGTIASLDA
                     LLAGTRAAVA"
     gene            1206..>2284
                     /locus_tag="PLQ18_10895"
     CDS             1206..>2284
                     /locus_tag="PLQ18_10895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNE02204.1"
                     /translation="MNSRTASSATRPAATSAADDLAEGFARHVVDWARQAGAPADSLA
                     IVATAARNASLATQDGHVCTRFDDLAAAHADHSPGEVLRRLLASHMVAVASEPRALPL
                     LIDDDGRIYLYRHFACELRLAANLRRRCAPLPAAPGEAARQQLDQLFAGNSQRDGARP
                     DWQKLAVALAWRGRLTIISGGPGTGKTTTVVALLACLLTENPHLRIALAAPTGKAAAR
                     MLEALRRRAASLPAELRALLPNESHTLHRLLGVTPETGRFRHHAENPLPIDALIVDEA
                     SMLDLALACRLCEAVPPAARLILLGDKDQLAAVEAGAVFAEISADPKLSAACLDELAT
                     LTATPAEQIRPPPAAAPTPLSDCVV"
BASE COUNT          336 a          855 c          743 g          350 t
ORIGIN      
        1 cgctgctacc tggtggccgg ctgttacgcc actgcgaact tcggccggcg ctcgctgacc
       61 cagggtacgc gcagcctgct caactggctg gttgccggcc gcaacctcgg ctactccgag
      121 tggcgagttc acgatctgcc gcctgccacc atcgagaatg cctggcaggc gctcgccgat
      181 gcggcggctc cgcagttgtc ccttgccgat ctgcccagcg cagcagccac cccgctcgct
      241 cgcgacgcgc tggccgccga cagcctcgcg gcgctggctc cacccggcag gctgccgccc
      301 ggctggcagc tcgcgagctt cagcagcctg caacacggcg ccgggcacga agccgcggcc
      361 tgcgatcacg acggccgcgg cgaacgctcg ccgatgtcag cggcgcgcag cgaagacagc
      421 gcaggcgaac tggcagccga cgacatcctg cactttccgc gcggagcggc cgccggcgat
      481 tgtctgcacg cgctgttcga agcaatcgac ttcaccgatc cggacggctg gccggatgcg
      541 atcgcgcgcg tgctcgccgc ccacccacag gcactgccgg gtctgccggc aactgaagcc
      601 gacaggcgcc tggcacgaat gctgcgtcgc ctgctcgacg acgtgctggc gacgacgctg
      661 ccgggcggca tcgtcctcgg cgcgctggga cgccgggggc ggctggtgga gctcggcttc
      721 cacctgtcca ccggagaaat cactccgaaa gtgctcaacg gctggctgca gaagcacggt
      781 tacgccatgc cgagactcgg cttcccgccg ctggcggggt acctcaaggg cttcatcgac
      841 ttggtcttct gtcacgccga ccgctactac gttctcgact ggaagtcgaa ccatctcggc
      901 tacacgtctg ccgactacga ttgcgcaccg atcgcggccg cgatggcgga gcacggctac
      961 cacctgcagt acctgatcta cacggtcgcc ctgcaccgct acctgcgccg gcgcatcccc
     1021 gattacgatt acgagcgcga tttcggcggc gtcctctacc tcttcgtgcg cggcgtccgc
     1081 cccgcctggc ggatggtggc tgccgacgga gaatcgatcg cctgcggcat ctaccatcac
     1141 cggccgagcg cgggcaccat cgcctcgctc gacgcgttgc tggcgggaac ccgcgccgct
     1201 gtcgcatgaa ctcgcgcacc gcttcctcgg cgaccagacc ggccgccacg agcgcagccg
     1261 atgacctcgc cgaagggttt gcgcgccatg tcgtcgactg ggccagacag gcgggagcgc
     1321 ccgcagacag tcttgccatc gtggcgacgg ctgcgcgcaa cgccagcctg gcgacgcagg
     1381 acggtcacgt ctgcacgcgc ttcgacgatc tcgcagcagc gcacgccgat cactcgccgg
     1441 gcgaagtgct tcggcgcctg ctcgccagcc acatggtggc ggtggcaagc gagccgcgcg
     1501 cactgccgct gctcatcgat gacgacggtc gcatctacct ctaccgtcat tttgcctgcg
     1561 agcttcgcct ggcggcgaat ctccggcggc gctgcgcacc gctcccggcc gcaccgggcg
     1621 aagcggcgcg gcagcagctc gatcagctct tcgccggaaa ttcgcagcga gacggcgccc
     1681 gtcccgactg gcagaagctg gcggttgcgc tcgcctggcg cggccggttg acgatcatca
     1741 gcggtggtcc gggaaccggc aagaccacca ccgtcgtcgc gctgctcgcg tgcctgctga
     1801 ccgagaaccc gcacttgcgc atcgcgctcg ctgctcccac cggcaaggcc gcggcgcgca
     1861 tgctcgaagc gctgcgccgg cgtgccgcca gcctgccggc agagctgcgg gcgctgctgc
     1921 cgaacgagtc gcacacgctg caccgcttgc tcggcgtcac cccggaaacc ggtcgctttc
     1981 ggcaccatgc ggaaaacccg ttgccgatcg acgcgctgat cgtcgacgaa gcgtcgatgc
     2041 tcgatctcgc gctggcttgc cggctgtgcg aggcggtacc gcccgccgcc cgcctgatcc
     2101 tgctgggcga caaggaccag ctcgcagccg tcgaagccgg cgcggtcttc gccgagattt
     2161 cggccgatcc gaagttgagc gctgcctgcc tcgacgaact cgccacgctg acagcgacgc
     2221 cggcggagca aatccggcca ccgccggcgg ctgccccgac gccgctttct gactgcgtcg
     2281 tctg
//
LOCUS       DAOQJH010000501         2279 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_35982_length_2279_cov_3.571942, whole genome shotgun sequence.
ACCESSION   DAOQJH010000501 DAOQJH010000000
VERSION     DAOQJH010000501.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2279
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35982_length_2279_cov_3.571942"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1142)
                     /gene="coxB"
                     /locus_tag="PLQ18_10900"
     CDS             complement(<1..1142)
                     /gene="coxB"
                     /locus_tag="PLQ18_10900"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005783424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HNE02205.1"
                     /translation="MSAFRPCGLAATGVLGLWSAAVHADFQLNMTPGVTAVSHRIYEL
                     HMLIFWICVAIGIVVFGVMFWSVYHHRKSRGAVAAQFHESTTIEIAWTIVPVLILVGM
                     AIPATKTLVQMEDTSNADLTIKVTGYQWKWRYDYLGEDIGFYSSLKTDARQIHGETDK
                     GEHYLLEVDRPLVVPVGRKVRILTTASDVIHAWWVPALGWKRDAIPGFVNESWAKIDQ
                     PGTYRGQCAELCGKDHGFMPIVVVAMNQPDYDKWLADTKAAAASERNSADRVWGKAEL
                     MAKGAEVYRSCAACHQASGEGIAGMFPPLIHGKPFSGPEALLEPLRARGFLSADGKIT
                     VGPLDKHAEIVLKGIPGTPMAGYAGQLSDVEVAAVLTYIRNSWGND"
     gene            complement(1272..1760)
                     /locus_tag="PLQ18_10905"
     CDS             complement(1272..1760)
                     /locus_tag="PLQ18_10905"
                     /inference="COORDINATES: protein motif:HMM:NF021517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2244 domain-containing protein"
                     /protein_id="HNE02206.1"
                     /translation="MVDVECHDEDHEVCFELRPNRSLSATGALLFFAATGCVCVTIAF
                     VCLYFGFWPVVPFTGLELAALGAALWWTQRDNRRRESIRIGAQQVIVERSGRRPMRVA
                     LTRCWVRVSLEPDASGRGSRLELGTHGRPVEIGGFLTEAERQALAGSLRARLRCGRHG
                     YP"
BASE COUNT          382 a          777 c          764 g          356 t
ORIGIN      
        1 aagtcattgc cccaggaatt gcggatgtag gtcagcacgg cggcgacctc gacgtccgag
       61 agctggccgg cgtagcccgc catcggcgtg ccggggatgc ccttcagcac gatctcggcg
      121 tgcttgtcga gcggcccgac cgtgatcttg ccgtcggccg acaggaagcc gcgcgcgcgc
      181 agcggttcga gcagcgcctc ggggccggag aacggcttgc cgtggatcag cggcgggaac
      241 atgccggcga tgccttcgcc actggcctga tggcaggccg cgcagctgcg gtagacctcg
      301 gcacccttgg ccatcagctc ggccttgccc cagacgcgat cggccgagtt gcgctcggag
      361 gcagcggcag ccttggtgtc ggcgagccat ttgtcgtaat caggctggtt catcgcgacg
      421 acgacgatcg gcatgaagcc gtgatccttg ccgcagagct cggcgcactg gccgcgatag
      481 gtgccgggct ggtcgatctt cgcccaggac tcgttgacga agcccgggat cgcatcgcgc
      541 ttccagccga gcgccggcac ccaccaggcg tggatcacgt ccgaggccgt ggtcaggatg
      601 cgcaccttcc tgccgaccgg cacgaccagc ggcctgtcga cttcgagcag gtagtgctcg
      661 cccttgtcgg tctcgccgtg gatctggcgg gcgtcggtct tcaggctcga atagaagccg
      721 atgtcctcgc cgaggtagtc gtagcgccac ttccactggt agccggtgac cttgatcgtc
      781 aggtcggcgt tggaggtgtc ttccatctgc accagcgtct tcgtggccgg gatcgccatg
      841 ccgaccagga tcagcaccgg cacgatcgtc caggcgatct cgatcgtcgt gctctcgtgg
      901 aactgcgccg ccactgcgcc gcgcgacttg cggtggtggt acacggacca gaacatcacg
      961 ccgaacacga cgatgccgat cgccacgcag atccagaaga tcagcatgtg cagctcgtag
     1021 atgcggtggc tgacggcggt gacgccgggc gtcatgttga gctggaagtc ggcgtgtacc
     1081 gccgcgctcc acagcccgag gacgccggtc gcggcgaggc cgcagggacg gaaggctgac
     1141 atgcccgatg tactcctcag gtggcgaccc gtacgtcgcg ggtccggagc gggtgtgtgc
     1201 agggcacccg ccacgatggt tgcaagcctt cggtcggcga ccgccggaaa gcgccggggc
     1261 cgtctcctta tttatgggta tccgtgcctg ccgcagcgca gccgcgcgcg cagcgagccg
     1321 gccagcgcct gtcgctcggc ttcggtcagg aagccgccga tctcgaccgg ccggccgtgc
     1381 gtgccgagtt cgagccggct gccgcgcccg ctcgcgtccg gctccagcga cacgcgcacc
     1441 cagcagcggg tcagcgccac gcgcatcggt cgccggccgc tgcgctcgac gatcacctgc
     1501 tgcgctccga tgcggatcga ctcgcgccgg cggttgtcgc gctgcgtcca ccacagcgcc
     1561 gcaccgagcg ccgccagctc caggccggtg aacggcacga ccggccagaa gccgaagtaa
     1621 aggcagacaa aggcgatcgt cacgcagacg cagccggtcg ctgcgaagaa cagcagcgcg
     1681 ccggtggcgg acagggaacg gttcggccgc agctcgaagc agacctcatg atcttcgtca
     1741 tggcattcaa cgtcgaccat gaccctcgct ccggcatcac ggatggtcac ggtgtagaac
     1801 gggggccaaa gaactgtcaa cggcgtgaaa ttcaatgacg caacgcagca ttcacgcaga
     1861 cattaccgat ttttttgaaa ccccttgcgg atcaatcccc cgccttccgg cagtgatgaa
     1921 ctggtttgcc gaagtttcat gcaacgcaag cgaagggtcg gagcgggacg cgaagccggc
     1981 gtggattcat tgaaaatttc cgaccgaaat ccttaccggt aacacacgaa actcccgacg
     2041 cgggacgacg cttgccggca acccgatcgc ctgcagcccg ccgggcactg cggcaacgtc
     2101 ccggccggcg cccggtccgc cggcggcgct tcagcgcatc agccagaact cggcgctggc
     2161 gctctggccg gcggcatcga cgacggtcac gcgcgcggcc ccctccccgt ccggctgcca
     2221 ggcgacgctg cgccgcatcg gcgagccggt gagcggcacg ccgttgacca gccagcgca
//
LOCUS       DAOQJH010000502         2274 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_36127_length_2274_cov_3.447950, whole genome shotgun sequence.
ACCESSION   DAOQJH010000502 DAOQJH010000000
VERSION     DAOQJH010000502.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2274)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2274)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2274
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36127_length_2274_cov_3.447950"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..434)
                     /locus_tag="PLQ18_10910"
     CDS             complement(<1..434)
                     /locus_tag="PLQ18_10910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02207.1"
                     /translation="MSGRLQGLGWGERAVLLAALLVLVSGGLLWGVGSASTRTYTQYI
                     ASTDSLGRARQALTEARLGLARLSAREPGLDRNVVLAPLVRAQQAARAGLAGQSGLTT
                     LAASQPPAGELAAEFAEFIRALDAFGLAALDADGLGAPGTALR"
     gene            complement(431..1318)
                     /gene="ehuB"
                     /locus_tag="PLQ18_10915"
     CDS             complement(431..1318)
                     /gene="ehuB"
                     /locus_tag="PLQ18_10915"
                     /inference="COORDINATES: protein motif:HMM:TIGR02995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0033286 - ATPase-coupled ectoine
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0033288 - ATPase-coupled hydroxyectoine
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0033294 - ectoine binding [Evidence IEA];
                     GO_function: GO:0033295 - hydroxyectoine binding [Evidence
                     IEA];
                     GO_process: GO:0033308 - hydroxyectoine transport
                     [Evidence IEA];
                     GO_process: GO:0051470 - ectoine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ectoine/hydroxyectoine ABC transporter
                     substrate-binding protein EhuB"
                     /protein_id="HNE02208.1"
                     /translation="MDTPGSSAPAGGLRRPVLIVAALLILGAAGLAMLALLPGEEDAW
                     TRIRRSGSVRIGHTAEAPYAWRTPDGRVTGEAPELARAVFAQLGIQHIEWVEVQFGSL
                     IPQLRTGRFDVIAAGMYVTPARERQIAFSHPSYCTHAALLVRHADAQRLRGYADLAQD
                     PGARLAVLAGAVESEAALAAGVPAGRIHAFPDPASAAQAVAGGEAEALALSAPSVQWL
                     ADRDPRLRRAAEAVTAAPPEGCGAFGFRQEDRELRRRFDAQLRAFLGTPAHAALVRPF
                     GFTPAELPPALQPAPERRP"
     gene            complement(1327..>2274)
                     /locus_tag="PLQ18_10920"
     CDS             complement(1327..>2274)
                     /locus_tag="PLQ18_10920"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE02209.1"
                     /translation="RLVLVGAPVHDSPALLRGIPCVDRPLRPRQVLAALASAVPGPST
                     PLPGVSRQPRLAGLHLLVIEDNETNRLLLEAMLEREGARLTLAVNGLRALASLTALGP
                     AQFDLVLTDLQMPEMDGYETARRIAALAPDLPVLALSAHALPDERERCLVAGMKEQLT
                     RPVSADALVDAVLRYCRRPPGAPAVPPAAAPERAVTEVRAAAGPAPLDWAAIEADFPG
                     RPDFLRRLIDTVLASNAGVPARIAEAQAAQDLGALGFVAHSLKGMSGTLHAGALRALA
                     TRVETLARRGDAGAWALCEALEGALAELLDALRARRRTL"
BASE COUNT          292 a          883 c          859 g          240 t
ORIGIN      
        1 cgcagcgccg tgcccggcgc gccgagtccg tcggcatcga gcgcggccag cccgaaggca
       61 tcgagcgcac ggatgaactc cgcgaactcc gcggccagct cgcccgcggg cggctgcgat
      121 gcggccagcg tcgtcagtcc gctctggccg gccagcccgg cgcgggccgc ctgctgcgcg
      181 cgcaccagcg gcgccagcac gacgttgcga tcgagccccg gctcgcgggc cgacagccgc
      241 gccagcccca gccgcgcctc ggtcagcgcc tgacgggcgc ggcccaggct gtcggtgctg
      301 gcgatgtact gcgtgtaggt gcgcgtcgag gcgctgccga cgccccacag caggccgccc
      361 gagaccagca ccagcagcgc cgcgagcagc accgcacgct cgccccagcc gaggccttgc
      421 agccgtccgc tcacggccgg cgctccggcg ccggctgcag cgccggcggc agttcggccg
      481 gcgtgaaacc gaacggccgc accagcgccg catgcgcggg cgtgccgagg aaggcgcgca
      541 gctgcgcatc gaagcggcgg cgcagttccc ggtcctcctg ccggaagccg aaggcgccgc
      601 agccctcggg cggcgccgcg gtgacggctt cggcggcgcg cctcagccgc ggatcgcgat
      661 cggccagcca ctgcacgctc ggcgccgaca gcgccagcgc ctcggcctcg ccgccggcca
      721 ccgcctgcgc ggcactcgcg ggatcgggaa acgcatggat gcgtccggcc ggcacgccgg
      781 cggcaagtgc agcctcactc tcgaccgcgc cggcgagcac cgcgagccgc gcgcccgggt
      841 cctgcgccag atcggcatag ccgcgcaggc gctgtgcatc ggcgtgacgc accagcagcg
      901 cggcgtgcgt gcagtagctc gggtggctga aggcgatctg ccgttcgcgc gccggggtca
      961 cgtacatgcc ggcggcgatg acatcgaagc gccccgtgcg cagctgcggg atcagcgagc
     1021 cgaactgcac ctcgacccat tcgatgtgct ggatgccgag ctgcgcgaac acggcgcgcg
     1081 cgagttcggg cgcctcgccg gtgacgcggc cgtcgggcgt acgccacgca tacggggctt
     1141 cggccgtgtg gccgatgcgc acgctgccgc tgcgccggat gcgcgtccag gcgtcctcct
     1201 cgccgggcag cagcgccagc atcgcgagcc cggccgcgcc caggatcagc agcgcggcca
     1261 cgatcagcac cggccggcgc aggccgccgg ccggcgcgga agacccgggc gtgtccaagg
     1321 agggctctac agcgtccgtc gccgcgcccg cagcgcgtcg agcagttcgg ccagcgcccc
     1381 ttcgagcgcc tcgcacagcg cccaggcccc ggcatcgccg cgccgcgcca gggtctcgac
     1441 ccgggtcgcg agcgcgcgca gtgccccggc gtgcagcgtg ccgctcatgc ccttgaggct
     1501 gtgcgcgacg aaaccgagcg cgccgagatc ctgcgcggcc tgcgcctcgg cgatgcgcgc
     1561 cggcacgccg gcgttgctgg cgagcacggt gtcgatgagc cggcgcagga agtcggggcg
     1621 ccccggaaag tcggcctcga tcgccgccca gtcgagcggg gccggtccgg cagcggccct
     1681 gacctcggtc acggcccgct ccggcgccgc ggccggcggg acggcgggcg cccccggcgg
     1741 cctgcggcag tagcgcagca cggcgtcgac cagcgcatcg gcgctgaccg ggcgcgtcag
     1801 ctgttccttc atgcccgcga ccaggcagcg ctcgcgctcg tccggcagcg catgcgcact
     1861 gagggcgagc accggcagat cgggagcgag cgcggcgatg cgccgggcgg tctcgtagcc
     1921 gtccatctcc ggcatctgca ggtcggtcag caccagatcg aactgcgccg ggccgagggc
     1981 cgtgaggctg gccagcgccc gcagcccgtt cacggccagc gtcagccgcg caccctcgcg
     2041 ctcgagcatg gcttcgagca gcaggcggtt ggtttcgttg tcctcgatca cgagcaggtg
     2101 caggccggcc agccgcggct ggcgggagac gccgggcagc ggcgtggacg gccccggcac
     2161 ggcgctggcg agcgcggcga gcacctggcg cgggcgcagc ggccggtcga cgcagggaat
     2221 gccgcgcagc agcgccggcg agtcgtgcac cggcgccccg accagcacca gccg
//
LOCUS       DAOQJH010000503         2265 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_36372_length_2265_cov_4.726697, whole genome shotgun sequence.
ACCESSION   DAOQJH010000503 DAOQJH010000000
VERSION     DAOQJH010000503.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2265
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36372_length_2265_cov_4.726697"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..481)
                     /locus_tag="PLQ18_10925"
     CDS             complement(<1..481)
                     /locus_tag="PLQ18_10925"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HNE02210.1"
                     /translation="MQGQAKAEVTRMCRLLGVSRSGVYAARRRPVRSAGQLGAQVQAA
                     FQASGACYGSRRLRAALQAEGVTVGRYRVRRLMREFGLKPAWRRKFVHTTDSRHELTV
                     AGNLLDRQFSPPAPDRAWTSDITCVRTRRGWLYLAAVMDLYSRRIVGWAMAPAMPAAL
                     "
     gene            complement(1078..1371)
                     /locus_tag="PLQ18_10930"
                     /pseudo
     CDS             complement(1078..1371)
                     /locus_tag="PLQ18_10930"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
     gene            complement(1328..1996)
                     /locus_tag="PLQ18_10935"
     CDS             complement(1328..1996)
                     /locus_tag="PLQ18_10935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010814505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02211.1"
                     /translation="MSSSGLHDRFCRLWHAHGGRDAGQVFAGLAAHYAEPQRHYHTLA
                     HIRRCLRTLDHYRPAVPQPDLVELALWCHDVIYAPAARDNEARSADWWRAQAAGFAPA
                     SVDLVADAILATTHRQPPTGAVAAWTVDIDLAGLALSRSSFARDGRKLRAERPELGEA
                     EYGAGERAFLSGLLARARIYYTEALYADGEARARENLRRRIATLTGARHAGAAGGLSA
                     LGTG"
     gene            complement(2016..>2265)
                     /locus_tag="PLQ18_10940"
     CDS             complement(2016..>2265)
                     /locus_tag="PLQ18_10940"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006922155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNE02212.1"
                     /translation="GAGERLAYTVYGDAVNLAARLEPLNKDYGTRVLLSEATRLAAGE
                     ARFAWRAVGEVRVRGRSVATPLYTVDAPEAAAPADDAG"
BASE COUNT          324 a          850 c          765 g          326 t
ORIGIN      
        1 ccagcgctgc cggcatggcc ggggccatcg cccagccgac gatccggcgc gagtagagat
       61 ccatgaccgc ggccagatac agccagccgc gccgggtgcg gacgcaggtg atgtcgctcg
      121 tccaggcccg atcgggcgcc ggcggcgaga actggcgatc gagcaggttt ccggccaccg
      181 tcaactcgtg acggctgtcg gtggtgtgca caaacttgcg ccgccacgcg ggcttgaggc
      241 caaactcgcg catcagccgg cgcacccggt agcgcccgac cgtcacgccc tcggcctgca
      301 acgccgcgcg cagacgccgg ctgccgtagc acgccccact ggcctgaaac gccgcctgca
      361 cctgcgcgcc gagctgcccg gcactgcgca ccgggcgccg gcgtgcggca tagaccccgg
      421 aacggctgac ccccagcaga cggcacatgc gtgtcacctc ggccttcgct tgcccctgca
      481 cgatcagccg ccggatcgct tccgttcgcg ggcaaagaag gccgaggctt ttttcaggaa
      541 gtcgttgtcc aggcgcagct cccggttctc ggcttccagc tggcggatgc gctgctgctc
      601 gggcaggcgg gccgtggctt gccgatgccg gcaccaccgg cctgttcggc gtcgtactgc
      661 gccagagagc gacgcacggc gctgtcgacc agaccctgct cgcggcagac ccgcgtcacg
      721 ctcaggccat cttcgcggat catcttgatg atctgcagct tgaactcggc atcgaagctg
      781 cgtcgggtac tcatggggct cgtgtcctcg tctcggtggt gggatttccc ctatccgggt
      841 gtccacggaa attgaaccac gacattcagt agcctcggcc atcgggtcga gtccgcggcc
      901 ggcgtaggtc agcaatgttc tgaccccgct cgcggcaccc gtgagaaacc cggagccctc
      961 caggccggca gcgccagtct ccgcggctgt caccgccacg ccgccgacgg cgccggcata
     1021 agcgggcagg gcgttgcccg gcgacaccgg cggtgcgacg acgaacccgc tcggatccct
     1081 gaaatcaatc gggttccctc ccacgtacgc ataccggttg atccccccgg ccaaccccac
     1141 cgggtccggc tgcgtgaagc gtccaagctc cggggcgtag tagcgggcgc ggtagtagac
     1201 gaggccggtg gcgtcgggct cgcggccgct gtagccgtag gtcggcaggc tgccggcgct
     1261 ggcgaggagc cggccgaagc tgtcaaacag tgctgcggcc gactgcgcga gcgaggggct
     1321 tccttcgtca gcccgtccca agggctgaaa gcccgccggc cgctccggca tgccgtgccc
     1381 ctgtcagcgt cgcgatccgc cgccgcaggt tttcccgcgc ccgcgcctcg ccatctgcat
     1441 acagcgcttc cgtgtaatag atgcgcgccc gcgccagcag ccccgacaga aacgcccgct
     1501 cccccgcacc gtattcggcc tccccgagtt ccggtcgctc ggcccgcagc ttcctgccgt
     1561 cgcgcgcgaa gctcgatcgc gacagcgcca gtccggccag atcgatatcg acggtccagg
     1621 ccgcgaccgc gcccgtcggc ggctggcggt gcgtggtggc gaggatggcg tcggcgacca
     1681 gatcgacgct ggccggcgcg aagcccgcag cctgcgcccg ccaccagtcg gcgcttctcg
     1741 cctcgttgtc gcgcgccgcc ggtgcgtaga tcacgtcgtg gcaccagagc gccagctcga
     1801 cgagatcggg ctgcggcacc gccgggcggt aatgatccag cgtgcgcagg cagcggcgga
     1861 tgtgcgcgag cgtgtggtaa tgccgctgcg gctcggcgta gtgcgcggcg agtccggcga
     1921 acacctgccc ggcgtcgcgt ccgccgtgcg cgtgccagag ccggcagaag cgatcgtgca
     1981 agcctgacga tgacatgacg gcacccctcc ccggctcagc ccgcatcgtc cgccggggcc
     2041 gcggcttcgg gtgcatcgac cgtatacagc ggcgtcgcca ccgagcgccc gcgcacgcgc
     2101 acctcgccga ccgcccgcca cgcgaagcgc gcctcgccgg ccgcgagccg cgtggcctcc
     2161 gacagcagca cgcgcgtacc gtagtccttg ttcagcggct ccaggcgcgc cgcgaggttc
     2221 acggcatcgc cgtagaccgt gtacgccagc cgctcgccgg ccccg
//
LOCUS       DAOQJH010000504         2257 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_36618_length_2257_cov_3.718892, whole genome shotgun sequence.
ACCESSION   DAOQJH010000504 DAOQJH010000000
VERSION     DAOQJH010000504.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2257
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36618_length_2257_cov_3.718892"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(316..1731)
                     /locus_tag="PLQ18_10945"
     CDS             complement(316..1731)
                     /locus_tag="PLQ18_10945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015276959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HNE02213.1"
                     /translation="MLATGLAACTVGPDYARPAAPVPGEYREADGWQRAQPADARLRG
                     DWWTIYGDPQLDALVREAEVSNQNLALAEASLRQAQALAREAGAALYPVAGVGAGVTR
                     ARSSAAGATGTAGGRTTSQYSLPLELSWEIDLWGRLRRGAEAGQATAEASAADLEAAR
                     LSVRAELVTNWLALRTLDARRRLLADTVEAYARALAMTRNRHAAGVASGAEVAQAETQ
                     LESTRAQALDLDASRATLEHAIAVLAGRPPATLRIAVREGPLPPVPALPAGLPSTLLE
                     RRPDIAAAERRVAAANAGIGVAEAAYYPSLNFSASAGFEAASLAKWISAPARVWALGP
                     QLALTLFDGGARAAQVEAAGASWQGTVASYRQTVLTGFQEVEDALATLRILAAEAAVQ
                     DRAVTAARRSLELTMNQYRAGILAYLGVVVVQAQALAAESSAIDLQGRRLAASVALIR
                     ALGGGWQAGDPSPALTRADTR"
     gene            complement(1886..>2257)
                     /locus_tag="PLQ18_10950"
     CDS             complement(1886..>2257)
                     /locus_tag="PLQ18_10950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNE02214.1"
                     /translation="VILLIGIVKKNAIMMIDVALDLERRHGVSPLVAIHAAARLRLRP
                     ILMTTLAALLGALPLAFGSGLGAELRRPLGLAIVGGLFVSQWLTLYTTPVVYLVLDRL
                     GRRLRRERSPARSAIHAPLPD"
BASE COUNT          303 a          832 c          855 g          267 t
ORIGIN      
        1 atctgacccc gctcgtcgca gacccaccat tcggtgccat gaccgaggtc gaaaccgcgc
       61 agcgtgtagc cgggcggcag atcggcagcg aggcggcggg cggtccggtc ggattcgatc
      121 ttcggggtgc ttgcggcagt ggcgttcatg gcatccgtgc aactctgcgg tgaggggctg
      181 ccgcacccgc gcggatgtgg cgcgacgatt ctgcttgtgc gtcgcggcag aaccaaggtc
      241 gggaagagaa acatgaccaa gtcgtaaccc ggcacgacgc tcctgacagc aggcgtcaca
      301 agctggcgct gcgcgtcagc gggtgtcggc ccgcgtcagc gccggcgagg gatcccccgc
      361 ctgccagcca ccgccgagcg cgcggatcag cgcgacgctg gccgcgagcc gccggccctg
      421 caggtcgatc gcgctgctct cggcggccag cgcctgcgcc tgcacgacga cgacgccgag
      481 gtaggcgagg atgccggcgc ggtactggtt catcgtcagc tccagcgagc ggcgcgcagc
      541 ggtgacggcc cgatcctgca ccgcggcctc ggctgcgagg atgcgcagcg tcgccagcgc
      601 gtcctcgacc tcctggaagc cggtcagcac cgtctggcga tagctcgcga ccgtgccctg
      661 ccagctcgca ccggccgcct cgacctgcgc ggcgcgggcc ccgccgtcga acagcgtcag
      721 cgccagctgc gggccgagcg cccagacccg ggccggcgcc gagatccact tcgcgaggct
      781 cgccgcctcg aagccggccg aggccgagaa attcaggctc gggtagtacg cggcctcggc
      841 cacaccgatg ccggcgttgg ccgccgcgac ccgccgctcg gccgcggcga tgtcgggccg
      901 gcgttcgagc agcgtcgagg gcaggccggc cggcagtgcg ggcaccggcg gcagcggacc
      961 ctcgcgcacg gcgatgcgca gcgtcgccgg cgggcggccg gcgagcacgg cgatcgcgtg
     1021 ttcgagcgtc gcacggctgg cgtcgagatc gagcgcctgc gcgcgcgtcg attcgagctg
     1081 ggtttcggcc tgcgcgacct cggcgccgga ggcgacgccg gcggcgtggc ggttgcgcgt
     1141 catggcgagc gcgcgcgcgt aggcctcgac ggtatcggcg agcagccggc gccgggcatc
     1201 gagcgtgcgc agcgccagcc agttcgtgac cagctcggcg cgcacgctca ggcgcgcggc
     1261 ctcgagatcg gccgcgctcg cctcggccgt cgcctgaccg gcctcggcgc cacggcgcag
     1321 ccgcccccac agatcgatct cccaggacag ctccagcggc agcgaatact ggctggtcgt
     1381 gcgcccgccg gccgtgccgg tcgcacccgc ggcgctgctg cgcgcgcgcg tgacgccggc
     1441 accgaccccg gccaccggat acagcgcggc gccggcctcg cgcgccagcg cctgcgcctg
     1501 acgcaggctg gcctcggcga gcgcgaggtt ctggttcgag acttcggcct cgcgcaccag
     1561 cgcatcgagc tgcgggtcgc cgtagatcgt ccaccagtcg ccgcgcagcc gcgcatcggc
     1621 cggctgcgcg cgctgccagc cgtcggcctc cctgtactcg cccggcaccg gcgcagccgg
     1681 gcgcgcgtag tcggggccga ccgtgcaggc ggcgaggccg gtcgcgagca cggcgacggc
     1741 cagacagcgc agggcggcgg ccgcaaagcc tgcggcaccg ctccggcagc ctgatcccgc
     1801 ctcccctcgt tcaccgtccg cagcgagggc tgcctgcggg caggagccgg cacccgacgg
     1861 aaagcgtgag gcgcggcagc gggcttcagt cgggcagcgg ggcatggatg gcgctccggg
     1921 cagggctgcg ctcgcggcgc aggcggcggc cgagacggtc gagcacgagg tagacgaccg
     1981 gcgtcgtgta cagcgtcagc cactggctga cgaacaggcc gccgacgatg gccaggccca
     2041 gcggccggcg cagttcggcg ccgaggccgc tgccgaaggc gagcggcagc gcgccgagca
     2101 gcgccgcgag cgtggtcatc aggatcgggc gcagccgcag ccgcgccgcg gcgtggatcg
     2161 cgaccagcgg cgagacgccg tggcggcgtt cgagatcgag cgccacgtcg atcatcatga
     2221 tcgcgttctt cttgacgatg ccgatcagca ggatcac
//
LOCUS       DAOQJH010000505         2256 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_36650_length_2256_cov_3.641527, whole genome shotgun sequence.
ACCESSION   DAOQJH010000505 DAOQJH010000000
VERSION     DAOQJH010000505.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2256
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36650_length_2256_cov_3.641527"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(95..268)
                     /locus_tag="PLQ18_10955"
     CDS             complement(95..268)
                     /locus_tag="PLQ18_10955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02215.1"
                     /translation="MDTDCPARPAETRESPESTTPAQPILVPRWLRVAMRPQPDSDNG
                     HRSGNRWQAARKH"
     gene            complement(457..624)
                     /locus_tag="PLQ18_10960"
     CDS             complement(457..624)
                     /locus_tag="PLQ18_10960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02216.1"
                     /translation="MSEKHRPSIPNRTAQAEAWEALTLFARQAQRQPAANAQAARCRP
                     PVSHPQQSGKD"
     gene            762..1709
                     /locus_tag="PLQ18_10965"
     CDS             762..1709
                     /locus_tag="PLQ18_10965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018285246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HNE02217.1"
                     /translation="MSAPAPVTPEQRERLLRLATRASVATALLLIVGKTVAWLMTGSV
                     SVLASLVDSVMDALASLVNLVAVRVSLAPPDAEHRFGHGKAEALAGLGQATFIAGSAL
                     FLCLQAVDRLLRPQPLTDIGVGAAVIVFAIVATAVLLAIQRHVIRRTGSTAIRADALH
                     YLTDLATNASILLALGLGHFGYDGLDPLFALGIAVYILKSAWEIGAEAVSLLMDHELP
                     AETKQRIVELAREQHGVLGVHDLRTRQSGHTIFVQLHLELADSLLLAVAHAIGDAVES
                     AIENEFPGAEILIHQDPVSAVAQERRRMLAHQMGERAGR"
     gene            1774..>2256
                     /locus_tag="PLQ18_10970"
     CDS             1774..>2256
                     /locus_tag="PLQ18_10970"
                     /inference="COORDINATES: protein motif:HMM:NF019956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HNE02218.1"
                     /translation="MSVPARVFISYSHEPAAQRPRVEALAAALKDHGIGVVIDHDYDT
                     TCTHWPRWCMEQTAKGVTDFILCICTDEYSRRIVGDVPADVGKGVYWEGSLFIAELYD
                     DKNNTRLIPVLLDGEPAASIPRFLAGWTQCPIRAFTPDDAGFALLLGIVRRTAPLCRA
                     A"
BASE COUNT          305 a          765 c          781 g          405 t
ORIGIN      
        1 ggaggcagtg gaagcgcgct tcgcagggcc ctgaaacgac cgacgccggt cggcaccggc
       61 gtcgttgcgg aaattcagga agaccgcggg cgggtcagtg cttgcgcgcc gcctgccagc
      121 ggttgccgct gcggtggccg ttgtcgctgt ccggctgcgg gcgcatggcc acgcgcagcc
      181 agcggggcac gaggatcggc tgggccggag tggtggattc cggggattcg cgggtttcgg
      241 ccgggcgggc cgggcagtcg gtgtccatct gggtatgcct cgggcgatgc gggcgggggg
      301 aggatcggat ggccctcagc atgcgccgct gttgcggcgg cgcctatccg gcgcccgccg
      361 taatctgcct gtcgtttgaa aactcgcgtt gcattttgat taacttcctg taaatccgca
      421 ctttgtgtcg tgcggagccg cgaactgttt cgcgtttcag tccttgccgg attgttgcgg
      481 atgtgaaaca ggcggtctgc aacgtgcagc ctgcgcattc gctgcgggct ggcgttgcgc
      541 ctgacgggcg aacagcgtca gcgcctccca ggcttcggcc tgcgcggtgc ggttcgggat
      601 gctcggacgg tgcttttcgg acatggctcc ggttccccgc tcggtggatg ccgctactct
      661 gccgatcgtc catgacgatc cgtcgcggcc ggtgccacct tgctgcattg caaggttccc
      721 cccgtacaat tcgcgccgcc cgcccgcgac cggagtccgc aatgtctgcc cccgcccccg
      781 tcactcccga gcagcgcgaa cgcctgctgc ggctcgcgac ccgtgcttcc gttgcgacgg
      841 cgctgctgct gatcgtcggc aagacggtgg cgtggctgat gaccggctcg gtgtcggtgc
      901 tggcctcgct ggtggattcg gtgatggatg cgctggcctc gctggtgaat ctggtcgcgg
      961 tgcgcgtgtc gctggcgccg cccgatgccg agcaccgctt cggccacggc aaggccgagg
     1021 cgctggccgg gctcgggcag gcgaccttca tcgccggttc ggcgctgttc ctgtgcctgc
     1081 aggcggtcga tcgcctgctg cgcccgcagc cgctgaccga catcggcgtc ggcgcggcgg
     1141 tgatcgtgtt cgcgatcgtc gcgaccgccg tgctgctggc gatccagcgc cacgtcatcc
     1201 gccgcaccgg ctcgacggcg atccgcgccg acgcgctgca ctacctgacc gacctcgcca
     1261 ccaacgccag catcctgctc gcgcttgggc tcggtcattt cggctacgac gggctcgatc
     1321 cgctgtttgc gctcggcatc gccgtctaca tcctcaagag cgcgtgggag atcggcgccg
     1381 aggccgtcag cctgctgatg gatcacgagc tgccggccga aaccaagcag cgcatcgtcg
     1441 agctggcgcg cgagcagcac ggcgtgctcg gcgtgcatga cctgcgcacg cgccagtccg
     1501 ggcacacgat cttcgtgcag ttgcacctcg aactcgccga ctcgctgctg ctcgcggtcg
     1561 cgcacgcgat cggcgatgcc gtcgaatcgg cgatcgaaaa cgaattcccc ggcgccgaaa
     1621 tcctgatcca tcaggacccg gtcagcgccg tcgcgcagga gcgccggcgc atgctcgcgc
     1681 atcagatggg cgaacgcgcc gggcgctgag cgttccgcgc gcgctgcttc ggctacgctc
     1741 gccgggcatt ccccgaccgg aagggagtcc gcgatgtccg tgcccgcgcg ggtgttcatc
     1801 agctacagcc acgagcccgc agcgcaacgg ccgcgggtcg aggcgctggc tgcagccctg
     1861 aaggatcacg gcatcggcgt cgtcatcgat cacgactacg acacgacctg tacccactgg
     1921 ccgcgctggt gcatggagca gacggcgaag ggcgtgaccg atttcatcct gtgcatctgc
     1981 acggacgaat acagccggcg catcgtcgga gatgtgccgg ccgatgtcgg caaaggcgtg
     2041 tattgggaag gctcgctgtt catcgccgag ctgtatgacg acaagaacaa cacgcgcctg
     2101 atcccggtcc tgctcgatgg cgagcccgcc gccagcattc cgcgctttct tgctggatgg
     2161 acccagtgcc cgatccgggc attcacgccg gacgatgcgg gtttcgcgct gctgctcggc
     2221 atcgtccggc gcacggctcc gctctgccgg gcggcc
//
LOCUS       DAOQJH010000506         2256 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_36653_length_2256_cov_3.592912, whole genome shotgun sequence.
ACCESSION   DAOQJH010000506 DAOQJH010000000
VERSION     DAOQJH010000506.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2256
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36653_length_2256_cov_3.592912"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..481
                     /locus_tag="PLQ18_10975"
     CDS             <1..481
                     /locus_tag="PLQ18_10975"
                     /inference="COORDINATES: protein motif:HMM:TIGR02913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAF repeat/PEP-CTERM domain-containing protein"
                     /protein_id="HNE02219.1"
                     /translation="LGTLGGTTSTAAAINAGGLIAGYGQITGDTGTHPFLYSGGVMTD
                     LGTLGGDSDFLVALNAAGQGVGMSFLPGAAFPVGLFWDGGALRDLNALIDPASGWTIR
                     GATDINDAGWIVGYGNDGGIDTRALLLVPLAAIPEPDLLTLIGAGAWLLGRRRRETT"
     gene            840..1061
                     /locus_tag="PLQ18_10980"
     CDS             840..1061
                     /locus_tag="PLQ18_10980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014197158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02220.1"
                     /translation="MAAIAEHEHLPEVAAAGCGQELLQSAAGIFRLHDFIRDDIGVAR
                     VHGDTAHACHLEAVLSGFEHSHPESLRLH"
     gene            complement(1081..2196)
                     /locus_tag="PLQ18_10985"
     CDS             complement(1081..2196)
                     /locus_tag="PLQ18_10985"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003478727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HNE02221.1"
                     /translation="MSEPVDPSGLTVGIVGGGQLARMLALAGYPLGLHFIVLDPAKDA
                     CAGQVATQLHGEYDDPDALDELARRCDVVTFDFENVPAAALEHLAAEVPVAPNPRALG
                     TAQDRLAEKTLFRELDIPTPVYAAVDSREELSIAVATTGLPAVLKTRRFGYDGKGQRV
                     LRERADLDAAWAELAGHALILEGFVPFTRELSILAARSADGTTVFWPLVENVHTGGIL
                     RLSRAPADAEALEATARAHAQAVLDALDYVGVLAIEFFDVGGTLIANEMAPRVHNSGH
                     WTLEGAETSQFENHLRAVCGLPLGSTHAVGYSAMLNFIGRLPEPGPFLATPGLHWHDY
                     GKAPRSGRKVGHATLRTDQPDELEHALARLQPLLAAD"
BASE COUNT          325 a          746 c          846 g          339 t
ORIGIN      
        1 cctcggcacg ctcggcggca caaccagcac cgccgcagcg atcaatgccg gcggcctcat
       61 cgccggctac gggcagatca ccggcgacac cggcacgcat ccgttcctct acagcggcgg
      121 cgtgatgacg gatctcggca cgctcggcgg cgacagcgac tttctggtgg cgctgaacgc
      181 cgccggtcag ggcgtcggca tgagcttcct gccgggagcg gcgtttccgg tgggcctgtt
      241 ctgggacggc ggagccttgc gcgacctgaa tgcgctgatc gatccggcct cgggctggac
      301 gatccgggga gcgaccgaca tcaacgatgc cggctggatc gtcggctacg gcaacgacgg
      361 cggcatcgac acgcgcgcgc tgctgctggt gccgctggcg gcgattccgg aaccggacct
      421 gctgacgctg atcggggccg gggcgtggct gctcgggcgc cgccggcggg agacgacgtg
      481 agggcggagg tcgatcacgc ttcaatgcgc ggtttgcgcc cgctttcccc aggccctcac
      541 ccgtgagcgg agagggactg aaaagacggt gtgttcggcc gtgatcggca tgaggccggg
      601 gccgtacgtc tgcccgcggc cccggcggga cggggcgact gccggccggg gcctgcggcc
      661 cgcattgcca tatggtcatg aaacttcgct tgtgcccggt cagaccacgg cgcataagcg
      721 ccgctgcgca gcgtctgttc cagccccgct accccgggga cggcaggctc ttcgtcagga
      781 agcccatcat gttgtcactg caggactgtt gcgatttctg ttcgctttcc gaagacgaag
      841 tggcggccat cgccgagcac gaacatctgc ccgaggtcgc ggcggccggc tgcgggcagg
      901 agctgctgca gtcggcggcc ggcatcttca ggctgcacga cttcatccgc gacgacatcg
      961 gcgtcgcccg ggtgcacggc gataccgcgc atgcctgcca tctcgaagcg gtgctgagcg
     1021 gcttcgaaca cagtcacccg gaatcgctgc gtctgcactg agcgcccgcc ggcgccatgt
     1081 tcagtcagcg gcgagcaggg gctgcagccg cgccagtgcg tgctcgagtt cgtccggctg
     1141 atcggtgcgc agcgtggcgt gaccgacctt gcgcccgctg cgcggggcct tgccgtagtc
     1201 gtgccagtgc aggccgggcg tggcgagaaa cggccccggc tcgggcagcc ggccgatgaa
     1261 gttcagcatc gcgctgtagc cgacggcgtg cgtcgagccg agcggcaggc cgcagacggc
     1321 gcgcaggtgg ttctcgaact gcgaggtctc ggcgccttcg agggtccagt gcccggagtt
     1381 gtgcacgcgc ggcgccatct cgttggcgat cagcgtgccg ccgacatcga agaactcgat
     1441 cgccagcacg ccgacgtaat cgagcgcatc gagcacggcc tgcgcgtggg cgcgggccgt
     1501 ggcttcgagg gcttcggcgt cggccggcgc gcgggacagg cgcaggatgc cgccggtgtg
     1561 gacgttctcg accagcggcc agaacacggt cgtgccgtcg gcactgcgcg cggcgaggat
     1621 cgacagctcg cgcgtgaacg gcacgaagcc ttcgaggatc agcgcatggc cggcgagttc
     1681 ggcccaggcg gcatcgaggt cggcgcgctc gcgcagcacg cgctggccct tgccgtcgta
     1741 gccgaagcgg cgcgtcttca gcaccgccgg caggccggtg gtggcgacgg cgatcgacag
     1801 ttcctcgcgg ctgtcgacgg cggcatacac cggtgtcggg atgtcgagtt cgcggaacag
     1861 ggtcttttcg gcgaggcggt cctgcgcggt gccgagcgcg cgcgggttcg gcgcgaccgg
     1921 gacttcggcg gcgagatgtt cgagcgccgc ggccggcacg ttctcgaagt cgaaggtgac
     1981 cacgtcgcag cggcgcgcca gctcatcgag cgcgtccgga tcgtcgtatt cgccgtgcag
     2041 ctgcgtggcg acctgaccgg cgcaggcatc cttcgccgga tcgagcacga tgaagtgcag
     2101 gccgagcgga tagccggcga gcgccagcat gcgggcgagc tggccaccgc cgacgatgcc
     2161 gaccgtcagg ccggaaggat cgacgggctc actcacagcg gcagcctcgg gtcgggatcg
     2221 gcgagcacgg tgtcggtctg gcgggagcgg aaggcg
//
LOCUS       DAOQJH010000507         2256 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_36655_length_2256_cov_3.398001, whole genome shotgun sequence.
ACCESSION   DAOQJH010000507 DAOQJH010000000
VERSION     DAOQJH010000507.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2256
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36655_length_2256_cov_3.398001"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..558)
                     /gene="coq7"
                     /locus_tag="PLQ18_10990"
     CDS             complement(<1..558)
                     /gene="coq7"
                     /locus_tag="PLQ18_10990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020506121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinone
                     monooxygenase"
                     /protein_id="HNE02222.1"
                     /translation="MPTRRQYSPLDHLFGTLDQAVRTVFGPPAVTERPNPAAGEQSGP
                     LSEAERREAMRLMRVNHTGEICAQALYQGQALTARLPEVRGQMERAAAEEYDHLDWCE
                     RRVQALGGHTSVLNPLFYAASFTIGALAGRAGDRWSLGFVAETEHQVVRHLDSHLERL
                     PAQDTESRAIVGQMKVDEHRHAEHAL"
     gene            702..1022
                     /locus_tag="PLQ18_10995"
     CDS             702..1022
                     /locus_tag="PLQ18_10995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010090546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HNE02223.1"
                     /translation="MASHYRHHVFFCTNLREGDERPSCQRCNASAARDHAKARVKALG
                     LAGPGKVRINNAGCLDRCEEGPVIVVYPEAVWYTYVDHDDIDEIVDEHLANGRIVERL
                     RLPD"
     gene            1263..1691
                     /gene="rplM"
                     /locus_tag="PLQ18_11000"
     CDS             1263..1691
                     /gene="rplM"
                     /locus_tag="PLQ18_11000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HNE02224.1"
                     /translation="MKTFSAKPADVKRDWFVVDATDKTLGRLSTEIARRLRGKHKAEY
                     TPHVDTGDYIVVVNAEKIRVTGNKAQDKVYYKHTGYIGNMKAFTFEKMIERAPQRVLE
                     IAVKGMLPKNPLGRAMFRKLKVFAGPTHPHAAQQPQALDI"
     gene            1724..2119
                     /gene="rpsI"
                     /locus_tag="PLQ18_11005"
     CDS             1724..2119
                     /gene="rpsI"
                     /locus_tag="PLQ18_11005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011761177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HNE02225.1"
                     /translation="MADNQFYGTGRRKTSTARVFLRTNGKGDIVVNGRPLDIYFGRET
                     SRMVVRQALEAVDALGRFDFYITVAGGGMTGQAGAIRHGITRALMAYDESLRPTLRKA
                     GFVTRDARAVERKKIGLRKARRAVQFSKR"
     gene            2183..2256
                     /locus_tag="PLQ18_11010"
     tRNA            2183..2256
                     /locus_tag="PLQ18_11010"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2215..2217,aa:Gln,seq:ctg)
BASE COUNT          383 a          822 c          718 g          333 t
ORIGIN      
        1 gagcgcgtgc tcggcgtggc gatgctcgtc gaccttcatc tgcccgacga tcgcgcggct
       61 ctcggtgtcc tgcgcgggca ggcgttcgag gtggctgtcg aggtggcgca cgacctgatg
      121 ctcggtctcg gcgacgaagc cgaggctcca gcggtcaccg gcgcgtccgg ccagcgcgcc
      181 gatcgtgaac gacgcggcgt agaacagcgg attcagcacg ctggtatgcc cgccgagcgc
      241 ctgcacgcgc cgctcgcacc agtcgagatg gtcgtattcc tcggccgccg cgcgctccat
      301 ctggccgcgc acctcgggca gccgcgcggt cagcgcctgc ccctgataca gcgcctgcgc
      361 gcagatctcg ccggtatggt tgacgcgcat cagccgcatc gcctcgcgcc gctcggcctc
      421 gctgagcggg ccggactgct cgccggccgc cgggttcggg cgttccgtga ccgccggcgg
      481 tccgaacacc gtgcgcacgg cctgatcgag cgtgccgaac aggtggtcga gcggtgagta
      541 ctggcgacgg gtgggcatgg cggcgggctc ctgttgcagc gcaaggtccg gccatcctaa
      601 gcccggtgcc gcaccggcgg aaacccggca cggcggtgct ggccgcctgc cggcaccggc
      661 gcgatactcg ggcgctcccc gccgctcacc ggagcccccc catggccagc cactaccgcc
      721 accacgtgtt cttctgcacc aacctgcgcg aaggcgacga gcgcccgagc tgccagcgct
      781 gcaacgccag cgccgcgcgc gatcacgcca aggcgcgcgt caaggcgctc ggcctcgccg
      841 gccccggcaa ggtgcgcatc aacaacgccg gctgcctcga ccgctgcgag gaagggccgg
      901 tcatcgtcgt ctacccggag gcggtctggt acacctacgt cgatcatgac gacatcgacg
      961 agatcgtcga cgagcacctc gccaacggcc gcatcgtcga gcgcctgcgc ctgccggact
     1021 gaaatcacag caaaatcagc cctccgcgca cgcagcgcgc ccggacaaaa ggcttgtcac
     1081 ccgcgccggg cctgattact atgcgcgccc tcgaccggag cgcaggccct cgtgccgtgc
     1141 ccggacaccg aaccgatcca gattccatca gatcgaaaca gaattcccga gtcccgcacc
     1201 agcagcgcgg tcgacgccgg cagcggcagc gatccggatc ggggctcatt tccggagtga
     1261 acatgaagac cttcagcgcc aagccggcgg acgtcaagcg tgactggttc gtcgtcgatg
     1321 ccaccgacaa gaccctcggc cgcctgtcga ccgagatcgc ccgccgcctg cgtggcaagc
     1381 acaaggccga gtacacgccg cacgtcgaca ccggtgacta catcgtcgtc gtcaacgccg
     1441 agaagatccg cgtcaccggc aacaaggcgc aggacaaggt ctactacaag cacaccggct
     1501 acatcggcaa catgaaggcc ttcaccttcg agaagatgat cgagcgcgcc ccgcagcgcg
     1561 tgctcgagat cgccgtgaag ggcatgctgc cgaagaaccc gctggggcgt gcgatgttcc
     1621 gcaagctcaa ggtcttcgcc ggcccgaccc acccgcacgc ggcccagcag ccgcaggccc
     1681 tcgacatctg agccccgcgg cttccgagac aaggtttcag gacatggctg acaatcagtt
     1741 ctacggcacc ggccgtcgca agacctcgac cgcccgggtg ttcctgcgca ccaacggcaa
     1801 gggcgacatc gtcgtcaacg gccgtccgct cgacatctac ttcggtcgcg agacctcgcg
     1861 catggtcgtg cgccaggcgc tcgaagccgt cgacgcgctg ggtcgcttcg acttctacat
     1921 caccgtcgcc ggtggcggca tgaccggcca ggccggcgcg atccgccacg gcatcacccg
     1981 tgcgctgatg gcctacgacg agtccctgcg tccgacgctg cgcaaggccg gcttcgtgac
     2041 ccgcgatgcc cgtgccgtcg agcgcaagaa gatcggcctg cgcaaggccc gtcgcgcggt
     2101 gcagttctcg aagcgctgat gcaatattcg taccggtctt gcagtttcgc actgcaatcg
     2161 gtacgatacg cggcacgttt cttgggggat cgtctagcgg caggactacg gactctgact
     2221 ccgtcaacct aggttcgaat cctagtcccc cagcca
//
LOCUS       DAOQJH010000508         2246 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_36949_length_2246_cov_4.742583, whole genome shotgun sequence.
ACCESSION   DAOQJH010000508 DAOQJH010000000
VERSION     DAOQJH010000508.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2246
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36949_length_2246_cov_4.742583"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1587
                     /locus_tag="PLQ18_11015"
     CDS             <1..1587
                     /locus_tag="PLQ18_11015"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNE02226.1"
                     /translation="TNLYLRGANAGQTLLLVDGIRLGSSTLGSPAWQLLPLGLAERIE
                     VVRGAQSGLYGSDAIGGVVQVLTLPAPGSPVRPWAEAGLGTQQGRHLGAGVRGSQGAT
                     TFSLGGDWLSSDGFDATATDTFAHNDDRDGFRSGAGHVAVRQDVAPGQSFSFTALRAQ
                     GENRYDGSNPLTFATADHAEGKFVQQVLALNADNRLTDAWRMKTTLGQSRDQNEDFAD
                     GLSAGAFDTRRDQFTWQHEFRLADGHLLSLGAEWLHEKVGGSGVVDYAQKTRTTRALF
                     LVDAVTLGAHDLQLSLRSDDIDDIGRHNTGRLGWGWSFAPGLRATASYATGFKAPTFN
                     DLYYPAGPFAAGNPDLEPESSHGWDLGLEQHAGAWHWRLGGYRTRVGNLIQWVPDEFF
                     RFSPVNVANARLTGIEASLGWQDHGWQVNLDADWLRAEDTDSGDPLPNRPNRSARLSI
                     DYAPGTWSAGATLLAAAYPDDLPQRGWSVLDLRSGYRLDKDFELKLKVNNVFDRDYQT
                     VNGYPMPGREAWIAIEYGIR"
     gene            1839..>2246
                     /locus_tag="PLQ18_11020"
     CDS             1839..>2246
                     /locus_tag="PLQ18_11020"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014502064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HNE02227.1"
                     /translation="MGHRLSKIYTRTGDAGTTGLGDGTRVPKDALRIEVMGECDELNA
                     VLGMVLALPVREPIRAVLTEVQHRLFDLGGELCMPGYASLSAEDVTQLETDLDGFNAA
                     LPALKEFILPGGGPAAAACHLAREDQAINKAPSN"
BASE COUNT          343 a          857 c          753 g          293 t
ORIGIN      
        1 accaatctct atctgcgcgg cgccaacgcc ggccagacgc tgctgctggt cgacggcatc
       61 cgcctcggct cctcgacgct cggctcgccg gcctggcagc tgctgccgct cgggctcgcc
      121 gagcgcatcg aggtcgtgcg cggcgcgcag tccggcctgt acggctcgga tgcgatcggt
      181 ggtgtcgtgc aggtgctgac gctgccggcg cccggcagcc cggtgcggcc ctgggccgaa
      241 gccggtctcg gcacgcagca gggacggcat ctcggcgccg gcgtgcgcgg cagccagggc
      301 gcgacgacct tctcgctcgg cggcgactgg ctcagcagcg acggcttcga tgccaccgcc
      361 accgacacct tcgcgcacaa cgacgaccgc gatggcttcc gcagcggtgc cggccacgtc
      421 gccgtgcgtc aggacgtggc gccgggccag tccttcagct tcacggcgct gcgcgcgcag
      481 ggcgagaacc gctacgacgg cagcaacccg ctcaccttcg cgaccgccga tcacgccgaa
      541 ggcaagttcg tgcagcaggt gctggcgctc aatgccgaca accggctgac ggatgcctgg
      601 cgcatgaaga ccacgctcgg ccagagccgc gaccagaacg aggacttcgc cgatgggctc
      661 tcggccggcg ccttcgacac gcgccgcgac cagttcacgt ggcagcacga gttccggctc
      721 gccgacggcc acctgctgag cctcggcgcc gagtggctgc acgagaaggt cggcggcagc
      781 ggcgtcgtcg attacgcgca gaagacgcgc acgacgcgcg cgctgttcct cgtcgatgcc
      841 gtgacgctcg gcgctcacga cctgcagctc agcctgcgca gcgacgacat cgacgacatc
      901 ggccgccaca acaccggccg cctcggctgg ggctggagct tcgcgcccgg cctgcgcgcc
      961 acggccagct acgcgaccgg cttcaaggcg ccgaccttca atgatctcta ctacccggcc
     1021 ggcccgttcg ccgccggcaa cccggacctc gaacccgagt cctcgcacgg ctgggacctc
     1081 ggcctggagc agcacgccgg cgcctggcac tggcggctcg gcggctaccg cacccgcgtc
     1141 ggcaacctga tccagtgggt cccggacgag ttcttccgct tcagtccggt caacgtcgcc
     1201 aatgcccggc tgaccggcat cgaggcctcg ctcggctggc aggatcacgg ctggcaggta
     1261 aaccttgatg ccgactggct gcgcgccgag gacaccgaca gcggcgaccc gctgccgaac
     1321 cgcccgaacc gcagcgcccg cctcagcatc gactacgccc ccggcacctg gagcgccggc
     1381 gccacgctgc tcgccgccgc ctatccggat gacctgccgc agcgcggctg gagcgtgctc
     1441 gacctgcgct ccggctaccg gctcgacaag gacttcgagc tgaagctcaa ggtcaacaac
     1501 gtcttcgacc gcgactacca gaccgtcaac ggctatccga tgccggggcg cgaggcctgg
     1561 atcgcgatcg agtacggcat acgctagaac agcgcagtcg acgcacgcct cgcccggccg
     1621 tgatctgccg cgctcccccg cccccggtaa gctgccgcgt gcagcccctt ccccctcgcg
     1681 gggaaggggt cggggaaagg gggagagccg ccggcaccag cgccgaaacc atgcgccgcg
     1741 cgccatccct tgcggggggc agcgcggcgc ggcggccacg gccggcggag ctcctgccgg
     1801 ccgtggccga tccatctccc gcccgaggac tccccgcgat gggtcaccgc ctctcgaaga
     1861 tctacacccg caccggcgat gccggcacca ccggcctcgg cgacggcacg cgcgtgccca
     1921 aggacgcgct gcgcatcgag gtcatgggcg aatgcgacga actgaacgcc gtgctcggca
     1981 tggtgctggc gctgccggtg cgcgaaccga tccgcgccgt gctgaccgag gtgcagcacc
     2041 ggctgttcga cctcggcggc gagctgtgca tgcccggcta cgcatcgctg agcgccgagg
     2101 acgtgacgca gctcgaaacc gacctcgacg gcttcaacgc cgcactgccg gcgctgaagg
     2161 aattcatcct gcccggcggc ggcccggcgg ccgcggcctg tcatctggcg cgcgaggatc
     2221 aggccatcaa caaagcgccc agcaat
//
LOCUS       DAOQJH010000509         2240 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_37144_length_2240_cov_3.673227, whole genome shotgun sequence.
ACCESSION   DAOQJH010000509 DAOQJH010000000
VERSION     DAOQJH010000509.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2240)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2240)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2240
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37144_length_2240_cov_3.673227"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1028
                     /locus_tag="PLQ18_11025"
     CDS             <1..1028
                     /locus_tag="PLQ18_11025"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008618076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HNE02228.1"
                     /translation="DYHFALLPRMVRERLPEATIVTFWHIPWPNAESFSICPWRGEIL
                     RGLLGSSILGFHTQFHCNNFLSAVDRFLESRIDQEEATVTHGGETTLVRHYPISIEWP
                     PQAIRSLPPAAVCRAQVQERLGIGADVRLGVGVERFDYTKGILDRFLAVEALLVNHPE
                     WVGRFSFLQIAAPTRNRLPVYQQLRAEAEALAERINARYGRPNWRPIVLLAEHHSPVE
                     VFRLFRAADVCVVSSLHDGMNLVAKEFVAARDDERGVLVLSTFAGASRELLEALIVNP
                     YDTAGMARALVAGLSMSGAEQGERMRLMREIVKENNVYRWAGRMLLDAARIRKRGHLL
                     GWMETMN"
     gene            1152..1934
                     /gene="otsB"
                     /locus_tag="PLQ18_11030"
     CDS             1152..1934
                     /gene="otsB"
                     /locus_tag="PLQ18_11030"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010344368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HNE02229.1"
                     /translation="MTDPTYLPPPPVPDAPWALFLDVDGTLIELAVRPDAVAVPPELP
                     LLLNALDEALGGALALVSGRALAEVETLFAPHRYAIAGGHGAEWRPAADAAIECRPKP
                     ALAAARKRLEAFVCTHPGTLLETKPHALALHWRNAPAAESEALACMASVQAGLDSGFR
                     LLRGKSVCELLPEGFDKGAVVAGFCTSPPFAGRVPVFIGDDVTDEAGFAAVLARGGIA
                     VHVGTAGRPTCATRRLTDPAAVRAWLARDVLPACQRYGVPNP"
     gene            1931..>2240
                     /locus_tag="PLQ18_11035"
     CDS             1931..>2240
                     /locus_tag="PLQ18_11035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5911 domain-containing protein"
                     /protein_id="HNE02230.1"
                     /translation="MSDLNLGILGNCIIAALVDTEARIVWGCLPRLDGDPVFCALLDN
                     GPDPERGGYWSLTLPNGRTREQAYLPNTAVLRTVIGDAEGNAIEILDFAPRHKQYGRM
                     Y"
BASE COUNT          331 a          800 c          751 g          358 t
ORIGIN      
        1 aggactacca cttcgcgctg ctgccgcgca tggtgcgcga acgtctgccg gaggcgacga
       61 tcgtgacctt ctggcacatc ccgtggccga acgccgaatc gttcagcatc tgcccgtggc
      121 gcggggaaat cctgcgcgga ctgctcggca gttccatcct cggcttccac acgcagttcc
      181 actgcaacaa cttcctgtcg gcagtcgacc gcttcctgga aagccgcatc gatcaggaag
      241 aggcaacggt cacgcacggc ggtgagacga cgctggtgcg ccattatccg atctcgatcg
      301 aatggccgcc gcaggcgatc cgcagcctgc cgccggccgc ggtctgccgc gcacaggtgc
      361 aggaacggct cgggatcggc gccgacgtgc gcctcggcgt cggtgtcgag cgcttcgatt
      421 acaccaaggg catcctcgac cgctttctgg cggtcgaggc gctgctggtc aaccatccgg
      481 aatgggtcgg ccgcttctcg ttcctgcaga tcgccgcgcc gacacgcaac cgcctgccgg
      541 tctaccagca gttgcgtgcc gaggccgagg ccctggccga gcgcatcaat gcccgttacg
      601 gccggccgaa ctggcgaccg atcgtgctgc tcgccgagca tcacagtccg gtcgaagtgt
      661 tccggctgtt ccgtgccgcc gatgtctgtg tggtcagcag cctgcacgac ggcatgaacc
      721 tggtcgccaa ggagttcgtc gccgcgcgtg acgacgagcg cggcgtgctg gtcctcagca
      781 cctttgccgg ggcctcgcgc gaactgctgg aggcgctgat cgtcaatccc tacgacaccg
      841 ccggcatggc ccgggcgctg gtcgcggggc tgtcgatgtc cggggccgaa cagggcgaac
      901 gcatgcggct gatgcgcgag atcgtcaagg agaacaacgt ctatcgctgg gccgggcgca
      961 tgctgctcga cgccgcacgc atccgcaaac gcggtcatct gctcggctgg atggagacca
     1021 tgaactgaag ctgcgtccgc gcgccgaggg aacgatcggc gcgtgctgct ttctgcgcca
     1081 gggatgcgcg cgcagacctc aggccagcgg cactgccctt gctcaccccg tgcccccgag
     1141 cagagaagct gatgaccgat ccgacgtacc tgccaccgcc gcccgttccg gatgctccct
     1201 gggcgctgtt tctcgatgtc gatggcacgc tgatcgaact ggccgtgcgg cccgatgccg
     1261 ttgccgtgcc gccggagctg ccgctgctgc tgaacgccct cgacgaggcg ctcggcggcg
     1321 cactggcgct ggtcagcggc cgggccctgg ccgaggtcga aaccctgttc gcgccacacc
     1381 gctatgcgat tgccggcggc cacggcgccg aatggcggcc ggccgccgat gcggcgatcg
     1441 agtgcaggcc gaagccggcg cttgcggccg cgcgcaaacg gctcgaagcc tttgtctgca
     1501 cgcatccggg cacgcttctc gaaaccaaac cgcacgcgct ggcgctgcac tggcgcaacg
     1561 caccggcggc cgagtccgag gcgctcgcct gcatggcctc ggtacaggcc gggcttgaca
     1621 gcggtttccg gctgctgcgc ggcaagtcgg tctgcgagct gctgccagag ggcttcgaca
     1681 agggggcagt cgttgccgga ttctgcacca gcccgccttt cgccggccgt gtgccggtgt
     1741 tcatcggcga cgacgtcacc gacgaggccg gctttgccgc cgtgctcgcg cgcggcggca
     1801 tcgccgtgca tgtcggtacc gccggtcgcc cgacctgcgc cacccgccgc ctgaccgacc
     1861 ccgccgccgt gcgagcctgg ctcgcccgcg acgtgctgcc cgcctgccag cgttacggag
     1921 tcccgaaccc atgagcgacc tgaatctcgg cattctcggc aactgcatca tcgcggccct
     1981 ggtcgacacc gaggcccgca tcgtctgggg ctgcctgccg cggctcgacg gggatccggt
     2041 gttctgcgcg ctgctcgaca acgggccgga cccggagcgc ggcggctact ggagcctgac
     2101 gctgccgaac ggccgcacgc gcgagcaggc ctatctgccg aacaccgcgg tgctgcgcac
     2161 cgtgatcggc gacgccgaag gcaatgccat cgagatcctc gatttcgcgc cgcgccacaa
     2221 gcagtacggg cgcatgtacc
//
LOCUS       DAOQJH010000510         2240 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_37147_length_2240_cov_3.367506, whole genome shotgun sequence.
ACCESSION   DAOQJH010000510 DAOQJH010000000
VERSION     DAOQJH010000510.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2240)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2240)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2240
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37147_length_2240_cov_3.367506"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..335
                     /locus_tag="PLQ18_11040"
     CDS             <1..335
                     /locus_tag="PLQ18_11040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005760054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HNE02231.1"
                     /translation="VQVLCYEVRVALLALQESGEAPAPFDRLAAHDDMEQFYAHLEQV
                     LIEIGFHDPQRPRHLMRRLRRLFARAAVEQNELAILRGVLSAVQGGKQMRRTDAQLTQ
                     ISGEVPNS"
     gene            433..912
                     /locus_tag="PLQ18_11045"
     CDS             433..912
                     /locus_tag="PLQ18_11045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011629625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HNE02232.1"
                     /translation="MKLTTKGRYAVTAMLDLALHFHGEPVVLSDISERQNLSLAYLEQ
                     LFAKLRKQGLVASTRGPGGGYSLARSADAITLAEVIEAVDESVDATRCGGLGNCHGDH
                     QCLTHELWVELSSHIHAFLQGRTLGEMARRIGTRQPAVSTHPLRHFVQHDLRRAAGD"
     gene            1001..1972
                     /locus_tag="PLQ18_11050"
                     /pseudo
     CDS             1001..1972
                     /locus_tag="PLQ18_11050"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
     gene            complement(2092..2187)
                     /locus_tag="PLQ18_11055"
                     /pseudo
     CDS             complement(2092..2187)
                     /locus_tag="PLQ18_11055"
                     /inference="COORDINATES: protein motif:HMM:NF019356.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
BASE COUNT          325 a          793 c          808 g          314 t
ORIGIN      
        1 ccgtgcaggt actctgctac gaggtgcgcg tcgcgctgct ggcgctgcag gagagcggcg
       61 aggcgccggc accgttcgac cggctggcgg cgcacgacga catggagcag ttctacgcgc
      121 acctggagca ggtgctgatc gagatcggct tccacgatcc gcagcgcccg cgccacctga
      181 tgcgccggct gcgccggctg ttcgcgcgcg ccgcggtcga gcagaacgaa ctggcgatcc
      241 tgcgcggcgt gctgtcggcg gtgcagggcg ggaagcagat gcgccgcacc gatgcacagc
      301 tgacgcagat cagcggagaa gtgcctaata gttgaccgct tttctcgggt acgagtataa
      361 agcggcccac actaaagacg gtcctgctac gcacggggcc ggactatcga ggcggaagcg
      421 acgggggcag acatgaaact gacgacgaaa ggccgctatg cggtaaccgc gatgctggat
      481 ctcgcgctgc atttccacgg cgaaccggtg gtgctgtcgg acatctcgga acgtcagaac
      541 ctgtcgctcg cctacctcga acaactgttc gcgaagctgc gcaagcaggg cctggtcgcg
      601 agcacgcgcg gccccggcgg cggctattcg ctggcccgca gcgccgatgc gatcacgctc
      661 gccgaagtca tcgaggccgt cgacgagtcg gtcgatgcga cgcgctgcgg cggcctcggc
      721 aactgtcacg gcgatcacca gtgcctgacc cacgagctct gggtcgaact ctccagccac
      781 atccacgcct tcctgcaggg ccgcacgctc ggcgagatgg cgcggcgcat cggcacccgc
      841 cagccggccg tcagcacgca tccgctgcgt cacttcgtgc agcacgacct gcgccgcgcc
      901 gccggcgact gaggccggtg cgcgcaccga tgcgatgagc agcgccgtct atctcgatca
      961 cgccgcgacc acgccggtcg atgcgcaggt cgttgccgcg atgctcgaat gtctgggcgc
     1021 cgacggggac ttcggcaatc cggcctcgac cacccacgcc catggccggc gggctgccgc
     1081 gcgcgtcggg caggcacgcg ccgaactggc ggcgctgctc ggctgcgatc cggccgagct
     1141 ggtctggacc tccggggcca ccgaagccag caatctggcg ctgaagggcg cgctgcaggc
     1201 cagtcgccgc ggccggcacc tgatcacctg ttcgaccgaa cacaagtcgg tgctcgatgt
     1261 ctgcgcgcag ctgcgccgcg ccggcgccgg gctgaccgtg ctcgatcccg gcgccgacgg
     1321 cctgctcgat ccggcgcgac tcgccgcggc gctgcgcccg gacaccgcac tggtgtcggt
     1381 gatggccgtc aacaacgaga ccggcgtcgt gcaggacatc gccgcgctcg ggcggctcgt
     1441 gcgtgcgcac ggcgcgctgt tccacgtcga tgccgcgcag gcggccgggc gcatcgacct
     1501 gacggcatgg ctgcccgagc tcgacctgat gagtctgtcg gcgcacaagc tgtacgggcc
     1561 gaagggcatc ggcgcgctga acgtgcgccg gctgccgcgc gtgcggctcg atgcgcagat
     1621 gcacggcggc ggtcaggagc gcggcctgcg cgccggcacg ctggccgtgc accagatcgt
     1681 cggcttcggc acggcctgcc ggctgctgcg cgagcggcgc gaggccgacg cgcggcacgt
     1741 cgcggcgctc gatgaacggc tgcgggccgg gctctgtcac ctgcccggca cgcacctgca
     1801 cgggcatccg cagcagcgcg tcgccggcat cctgaacctc ggcttcgagg gcatcggcag
     1861 cgagagcctg ttcgccgcgc tgccggggct tgcgctgtcg ggcggctcgg cctgcagctc
     1921 ggccgaggcg cagccctcgc acgtgctgcg cgcgctgggc gtgccggatg cccccgcccg
     1981 ctgcagcctg cgcctgtcgc cggggcgatc gacgagcctc gccgacatcg atctggccat
     2041 cgcccggatc acgcggggca tcgacccggg gacggcgagc tcgcgggtgc agagcagcgg
     2101 cacgtcgctc atcccgagct cgcgggccgc gacggcgggg aactcggcgt cgagatcgtc
     2161 ggtgcaggtg agcagacagg agatcatcga ctcggttgcg aggtcgttgc gggcgatcat
     2221 ctcccgcagc agctcgtcgg
//
LOCUS       DAOQJH010000511         2239 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_37174_length_2239_cov_3.944597, whole genome shotgun sequence.
ACCESSION   DAOQJH010000511 DAOQJH010000000
VERSION     DAOQJH010000511.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2239)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2239)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2239
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37174_length_2239_cov_3.944597"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="PLQ18_11060"
     CDS             <1..193
                     /locus_tag="PLQ18_11060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02233.1"
                     /translation="RTPGRRVHGLVDGMRLVRAGRSLIFHIVRARSPMPKSMADYLAH
                     LAFYRDKGRISPTLPVMPE"
     gene            complement(213..632)
                     /locus_tag="PLQ18_11065"
     CDS             complement(213..632)
                     /locus_tag="PLQ18_11065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02234.1"
                     /translation="MPELRALLFPPEPRRFAGQRWLNVALRSVHLCAVAGLGAGFLHG
                     LPPASWQPWLWVALASGSALGLLFVWSDGRWLVQPPGLAVLAKLGLLGLAPFVPAAGA
                     WLFLGAVALSSVFAHAPARVRHCLLLPARWVPPQVRR"
     gene            complement(917..1720)
                     /locus_tag="PLQ18_11070"
     CDS             complement(917..1720)
                     /locus_tag="PLQ18_11070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02235.1"
                     /translation="MIASTLHAHRALLLAGVLAAASTGAGASVSFVATGPNTYSGGHT
                     LAAQAIFDLSGSVLSITLKNISTDTLDRPSDVLMGLFFDLAPPYTLSSPTATRSAGSS
                     YVNLATPSSSTNLGGEWAYRYSATGLSGSTVTAKYGVSGSGLGGTFGAGDRICSACPE
                     LSSGGGSAPDGLDYGIVSNSYVANTGSGVTSTVELVRNSISFMFNVAGGSILNLGAVI
                     SNVSFQYGTALDEPRLAGVKISEPASAALLALGGLLSCRKRRAMAGSAA"
     gene            complement(1953..2183)
                     /locus_tag="PLQ18_11075"
     CDS             complement(1953..2183)
                     /locus_tag="PLQ18_11075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02236.1"
                     /translation="MGSKLTTTARLDVSVPVLCRRQSVDTRLNRRIGVRIHFSLRIPA
                     AGSDCLDDEGMRVKKFYILLIFQMQICAASKA"
BASE COUNT          422 a          719 c          758 g          340 t
ORIGIN      
        1 gcggaccccg ggccggcgcg tgcacgggct ggtggacggc atgcgtctgg tgagggcggg
       61 ccgctccctg atcttccaca tcgtccgtgc ccgctcgccg atgccgaaga gcatggccga
      121 ctatctcgcc catctggcct tctaccggga caaggggcgc atctcgccga cgctgccggt
      181 catgccggaa taggccgggg gcacgccgcc ggctagcgcc ggacctgcgg cggcacccag
      241 cgcgccggca gcagcaggca atggcgcacg cgcgccgggg cgtgcgcaaa caccgacgag
      301 agcgcgaccg ccccgaggaa cagccaggcc ccggccgccg gcacgaacgg cgccagcccg
      361 agcagcccga gcttcgccag caccgccagc cccggcggct gcaccagcca gcgcccgtcg
      421 ctccacacga acagcagccc cagcgccgaa ccgctcgcca gggccaccca cagccagggc
      481 tgccagctcg ccggcggcag tccgtgcagg aaaccggcac cgagcccggc gaccgcgcac
      541 agatgcacgc tgcgcagcgc gacgttgagc cagcgctggc cggcgaagcg gcgcggttcg
      601 ggcggaaaca gcagggcgcg caactcgggc atcggcggat cgcaacaacg gggcggagcg
      661 agcatgttcg gtcgaggcag gcagcggcgt ccatccgccg gaaggggcgg acgatgcggc
      721 ggattgatcc gcaaactgca ggcgtgccgc ggttgccggg gcaggggcga atgacagaaa
      781 ccccggagcg ctgccgcgca tccggggttc atgcggtcat ggtgctgcgc tggatcgcgc
      841 cgccgccggg cagcgcgcct tcatttccag cccgcctgcc tcgcggcacg aggaagccgg
      901 ggcggacatt ccggattcag gctgctgacc cggccattgc gcgccgtttc cggcatgaaa
      961 gcagtccacc cagcgccagc agtgcagcac ttgccggttc ggagattttg actccggcaa
     1021 gtcgtggttc gtcgagtgcc gtgccgtact ggaagctgac gttgctgatc acggcgccga
     1081 gattcaggat gctgccgccc gccacgttga acatgaatga aatgctgttg cgcacgagtt
     1141 cgacagtgga cgtcacaccg ctgccggtat tggcgacgta actgttcgat acgatgccgt
     1201 agtcgagacc gtccggtgct gaaccaccgc ctgaactcag ttcgggacag gcgctgcaga
     1261 tccggtcgcc ggcaccgaag gtgccgccga gtccggaacc ggaaaccccg tatttggccg
     1321 tgacggtcga tccggacaat ccggtcgccg aataccggta cgcccattca ccgccgagat
     1381 tggtgctcga cgacggcgtg gcgagattca cgtaggaact gccggcactc cgcgtcgccg
     1441 tgggcgatga cagcgtgtag ggcggtgcga gatcgaagaa cagtcccatc agtacatccg
     1501 aggggcgatc gagagtgtct gtcgagatgt ttttcagcgt gatcgacagc acgctgcctg
     1561 acagatcgaa aatcgcctgt gccgccagcg tgtgtccgcc cgaataggta ttgggaccgg
     1621 tcgccacaaa actgacggag gctccggcac cggtactcgc ggcagcgagt acgccggcca
     1681 gcagcaacgc gcggtgcgcg tgcagggttg atgcaatcat cagaggtccc ctccgcgaag
     1741 ttcaccagga acgtgggcgg cggggcagag ggtgcgggta ccttccgggt ggggaatttc
     1801 accccgtaaa caaacagtcg cttccgtgtg tatccgtgtt caaccgaccg gcatctgcgc
     1861 cgcggcatgt accggggggc tgcgactgcc cgcccggctg gtaaggcagc aaaaaatgtt
     1921 gcaatggtta tgccggtcat ggcaagtgct tgtcaagctt tgcttgcggc gcagatttgc
     1981 atctgaaaaa tcagtaatat gtaaaatttt ttgacacgca tgccttcatc atcaaggcaa
     2041 tcgctgccgg cagcaggaat acgcaaggag aaatgaatcc ggacgccgat gcggcggttc
     2101 agccgggtat caacagactg acggcggcag aggaccggaa cgctgacatc aagccttgct
     2161 gtcgtcgtca gcttcgatcc catggcaagc gcaccttccc ggcatgcgaa aagtcagggg
     2221 ctgcgtgggt ggcaggcgc
//
LOCUS       DAOQJH010000512         2238 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_37206_length_2238_cov_3.018781, whole genome shotgun sequence.
ACCESSION   DAOQJH010000512 DAOQJH010000000
VERSION     DAOQJH010000512.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2238)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2238)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2238
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37206_length_2238_cov_3.018781"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1049
                     /locus_tag="PLQ18_11080"
     CDS             <1..1049
                     /locus_tag="PLQ18_11080"
                     /inference="COORDINATES: protein motif:HMM:NF015926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF349 domain-containing protein"
                     /protein_id="HNE02237.1"
                     /translation="RNAYRTLEQDWKAAAEAAGGRIEAERQRERARREALIVQLQSLA
                     DEDLRTAIAAARQAQADWSPTLQLRRDAEQALWLRFRAVCDAIFGRREQVRSAGQAQR
                     QATLDAAAALCAELETLAATPLDADNAGAARAAAARIAEDWAGLGELPRAAQHAIEQR
                     YAAALDAWHERLTGLERVQRRKAVHALAEKATLCARLERHVLDGPPATAGDPGADGAD
                     DEPAALREEWQRLARLPEPLERRIRSRLDAAQRALADPVEAHRLAMRMTEGASIRHRL
                     CLELEILAGIEPPPEDAQELLEHRIARLSAALSGEAPPDADSVIHDWYCTPAAADPAL
                     DTRFATALVALGQA"
     gene            complement(1140..>2238)
                     /locus_tag="PLQ18_11085"
     CDS             complement(1140..>2238)
                     /locus_tag="PLQ18_11085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02238.1"
                     /translation="APAAALLPVAPAAVAEAAQLRGIVAGAPLRLTGTLLDAGRLTRL
                     PLAPGEGSAAAAAPVRPEALIELARYARERGDFGAAGQALAAADARVQGPQGTAALAD
                     AVLLERIALTLTLPAAAAGTSAAPAAAGLPPLAERLAAARRLADGLFSDAARADACLL
                     LAASAPGPGQALADFERARDFVRLTEPAEDRAAASGRFARALMLAGRQEAGQQEFVLA
                     QSLADGLADRQARANALALLARLRVEAGDLAGAERLHARLGEPSPAGAHDAQWLAARA
                     GLRASAGELAAAQVDIAAAYDRLSALDDAAAAAAGLMYLARRLQAAGDSISAERLVAG
                     AMSRYAQDDGSVATATTVPAPPAAQPAAGSR"
BASE COUNT          279 a          885 c          849 g          225 t
ORIGIN      
        1 cgcgcaacgc ctaccggacg ctggagcagg actggaaggc cgccgccgag gccgccggcg
       61 ggcgcatcga ggccgagcgc cagcgcgagc gcgcccggcg cgaggcgctg atcgtgcagc
      121 tccagagcct ggccgatgaa gacctgcgca ccgcgatcgc cgccgcccgc caggcccagg
      181 ccgactggtc accgacgctg cagttgcgcc gcgatgccga gcaggcgctg tggctgcgct
      241 tccgggcagt ctgcgatgcg atcttcgggc gccgcgagca ggtgcgctcg gccggtcagg
      301 cgcagcggca ggcgacgctc gatgccgccg ccgcgctgtg cgccgaactg gaaacgctgg
      361 cagccacgcc cctcgatgcc gacaacgccg gcgccgcccg cgccgcggcg gcacgcatcg
      421 ccgaggactg ggccgggctc ggcgagctgc cgcgcgcggc gcagcacgcc atcgaacagc
      481 gttatgcggc cgccctcgat gcctggcacg agcggctgac cgggcttgaa cgcgtgcagc
      541 ggcgcaaggc cgtgcatgcc ctcgccgaga aggcgacgct ctgtgcgcgg ctggaacggc
      601 acgtgctcga cggcccgccc gccacggccg gggaccccgg tgccgacggc gccgacgacg
      661 agccggcggc gctgcgcgag gagtggcagc ggctggcgcg gctgcccgaa ccgctcgaac
      721 ggcgcatccg cagccggctc gacgccgcgc agcgcgcgct ggccgatccg gtcgaggccc
      781 accggctggc gatgcggatg accgagggcg cgtcgatccg ccaccggctg tgcctggagc
      841 tggagatcct cgccggcatc gaaccgccgc ccgaggacgc gcaggaactg ctcgaacacc
      901 gcatcgcccg gctgtcggcg gcactgtccg gcgaagcgcc gccggatgcc gacagcgtca
      961 tccacgactg gtactgcacc ccggccgccg ccgatccggc cctcgacacg cgctttgcga
     1021 ccgcgctggt cgcgctcggc caagcctgaa gcccgagcct ggccccctcc tccccggccc
     1081 ttcccccggc aggggaaggg tgaaaagcgg tggccggatc agggtccggc aaggtttcct
     1141 cagcggctgc cggcagccgg ctgcgccgca ggcggtgcgg gaaccgtcgt cgcggtggcg
     1201 acgctgccgt cgtcctgcgc atagcgcgac atcgccccgg ccaccagccg ctcggcgctg
     1261 atgctgtcgc cggcggcctg cagccggcgc gccagataca tcaggccggc cgccgcagcc
     1321 gcggcgtcat cgagcgcgct caggcgatcg taggccgctg cgatgtcgac ctgcgccgcc
     1381 gccagttcgc cggccgaggc gcgcaggccg gcgcgcgcgg ccagccactg cgcatcgtgc
     1441 gcgccggccg gcgacggttc gccgaggcgc gcatgcagcc gctcggcccc ggcgaggtcg
     1501 ccggcttcga cccgcaaccg cgccagcagc gccagcgcgt tcgcgcgcgc ctgccggtcc
     1561 gccagcccgt cggccagcga ctgcgcgagc acgaactcct gctgcccggc ctcctgacga
     1621 ccggcgagca tcagcgcccg ggcgaagcgg ccgctggcag cggcgcggtc ctcggccggc
     1681 tcggtcaggc gcacgaagtc gcgcgcgcgc tcgaagtcgg ccagcgcctg cccgggcccc
     1741 ggcgcgctgg cggccagcag caggcaggca tcggcccgcg ccgcatcgct gaacaggcca
     1801 tcggccagcc ggcgcgccgc cgcgaggcgt tcggccagag gcggcaggcc ggccgctgcc
     1861 ggcgcggcgc tggtcccggc agcggccgcg ggcagggtga gggtcagggc gatgcgttcg
     1921 agcagcacgg catcggcgag cgccgcggtg ccctgcgggc cctggacccg ggcatcggcc
     1981 gccgcgagcg cctgtccggc cgcgccgaag tcgccacgct cgcgcgcgta gcgcgccagt
     2041 tcgatcagcg cctcgggccg cacgggcgca gccgccgccg cgctgccttc gccgggcgcg
     2101 agcggcagcc gcgtcagacg gccggcatcg agcagcgtcc cggtcaggcg cagcggcgcc
     2161 ccggccacga tgccgcgcaa ctgcgccgcc tctgccaccg ctgcgggggc gaccggcagc
     2221 agcgcggcgg ccggggca
//
LOCUS       DAOQJH010000513         2234 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_37328_length_2234_cov_4.266636, whole genome shotgun sequence.
ACCESSION   DAOQJH010000513 DAOQJH010000000
VERSION     DAOQJH010000513.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2234)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2234)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2234
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37328_length_2234_cov_4.266636"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..842)
                     /locus_tag="PLQ18_11090"
     CDS             complement(<1..842)
                     /locus_tag="PLQ18_11090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009043998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02239.1"
                     /translation="MTEGSRETAAAADDLAYPEPLRDREQVLLERRRARAGVPDDAVR
                     LGMAFSGGGIRSATFCLGFTQALARARELRQVDYLSTASGGGYFGGFLGRLYARDYVA
                     GPDKVEAMLDPELPPSAAGVGRDDPAFRPNAFRWLRENGRYLAPNGAGDLLLAFAVAL
                     RNWAAMHLLLASGVLALLLLLQVPILWLPADGAVAAALPGSERLLWWSPLAALLPWVF
                     NFVLLPLGWAYWLIAAREGLDGKAVPALIDWRQPAVAGLLVLLCLFSGHVGAGIDLLL
                     VTGLA"
     gene            1013..1765
                     /gene="moeB"
                     /locus_tag="PLQ18_11095"
     CDS             1013..1765
                     /gene="moeB"
                     /locus_tag="PLQ18_11095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017475988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HNE02240.1"
                     /translation="MNDEELLRYSRQIMLPEIDIEGQERLRAATALVIGLGGLGSPVA
                     MYLGAAGVGRLLLVDFDHVDTSNLQRQIAHAEADIGRPKVESARDRVRALNPLVAIET
                     IAEALDDEALAAAVARADVVLDCTDNLTTRLAINAACVAAGRPLVTGAAIRMEGQAMV
                     VDPRVPEAPCYRCLYRSAEEPAESCTQSGVLAPVVGIIGSLQAVEAIKVLTGAGEPLT
                     GRLLLMDARTMEFHEVRVPKYAGCPVCGTQSG"
     gene            1976..>2234
                     /locus_tag="PLQ18_11100"
     CDS             1976..>2234
                     /locus_tag="PLQ18_11100"
                     /inference="COORDINATES: protein motif:HMM:NF016668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HNE02241.1"
                     /translation="MSAGIALVVNADDYGYFDCVSRGILAAHAAGSVGATGVMANGPR
                     FETTAARLRATPALDAGVHLTLSWGEPLTRALAARLAEGRLP"
BASE COUNT          338 a          787 c          804 g          305 t
ORIGIN      
        1 gcaagacccg tgaccagcag caggtcgatg ccggccccga catgaccgga aaacaggcag
       61 agcagtacca gcagcccggc caccgccggc tggcgccagt cgatcagtgc cggtaccgcc
      121 ttgccgtcca ggccttcgcg cgcggcgatg agccagtacg cccagccgag cggcagcagc
      181 acgaagttga acacccaggg cagcagcgcc gcgagcggac tccaccagag caggcgctcg
      241 ctgccgggca gggcggcagc cacggcacca tcggccggca gccacaggat cggcacctgc
      301 agcagcagca gcagcgccag cacgccgctt gcaagcagca ggtgcatcgc cgcccagttg
      361 cgcagcgcca cggcgaacgc gagcagcagg tcaccggcac cgttcggtgc caggtagcgg
      421 ccgttctcgc gcagccagcg gaaggcgttc ggacggaagg ctggatcatc gcgaccgacc
      481 ccggcggcgg agggcggcag ttccgggtcg agcatggcct cgaccttgtc gggaccggcc
      541 acgtagtcgc gcgcatacag ccggccgagg aagccgccga agtagccacc gcccgaggcg
      601 gtcgacagat aatcgacctg gcgcagctcg cgggcgcggg cgagggcctg tgtgaagccg
      661 aggcagaagg tcgcgctgcg gatgccgccg ccggagaatg ccatgccgag gcgcacggca
      721 tcgtccggca cgccggcccg cgcgcgccgg cgttcgagca gcacctgttc gcgatcgcgc
      781 aatggttccg gataggcgag gtcgtcggca gccgcagcgg tttcgcggga tccttcggtc
      841 atggcatagc ccctggtggc gatccgggcc ggcggccgtc cgtgtgtgct ggggccgcgc
      901 gtgctgaagc cgggtgtctc aggggcagta aagccccggg cttgccggct gcccttaact
      961 ttttttcaga attgaacccc gccggctgcg gccggtcgcc ggagtccctg cgatgaacga
     1021 cgaagaactg ctgcgctaca gccgccagat catgctgccc gagatcgaca tcgagggtca
     1081 ggagcgcctg cgcgcggcga ccgcgctggt gatcggcctc ggcggcctcg gctcacccgt
     1141 ggcgatgtat ctcggcgcgg ccggggtcgg gcggctgctg ctggtggatt tcgatcacgt
     1201 cgacaccagc aacctgcagc gccagatcgc acacgccgag gccgacatcg gccggcccaa
     1261 ggtcgaatcg gcccgcgacc gcgtgcgggc gctgaatccg ctggtcgcga tcgagaccat
     1321 cgccgaggcg ctcgatgacg aggcgctggc cgcggcggtg gcgcgggccg atgtcgtgct
     1381 cgactgcacc gacaacctga cgacgcgcct ggcgatcaac gccgcctgcg tggcggccgg
     1441 ccggccgctg gtgaccggtg cggcgatccg catggaaggg caggcgatgg tcgtcgatcc
     1501 gcgcgtgccg gaggcgccgt gctaccgctg cctgtaccgc tcggccgagg agccggccga
     1561 gagctgcacg cagtccggcg tgctggcgcc ggtggtcggc atcatcggca gcctgcaggc
     1621 ggtcgaggcg atcaaggtgc tgaccggggc cggcgagccg ctgaccggcc ggctgctgct
     1681 gatggatgca cgcaccatgg aattccacga ggtgcgggtg ccgaaatacg ccggctgtcc
     1741 ggtctgcggc acgcagtccg gctgaggccg gaatcctcgc agcggaaatc cgaaaattct
     1801 tttgttttcg gcagcttctg gtcattgtgc gggacatttg cgtcgggcat gatgcgcgcc
     1861 cgtcgtgccc ctgcgcacgc cactgccggc ccgccgtcgc catgaccgaa gccctgcctg
     1921 cgcccgctgc caccgtcccc gctgccgccc ggatcgcggc acggacggtc cacgcgtgag
     1981 tgccggcatc gcgctggtcg tcaacgccga cgactacggc tatttcgact gcgtcagccg
     2041 cggcatcctc gccgcgcacg ccgccggcag cgtcggcgcc accggcgtca tggccaacgg
     2101 tccgcgcttc gaaaccacgg cagcgcgcct gcgcgccacg ccggcgctcg atgccggcgt
     2161 gcatctgacg ctgagttggg gtgaaccgct gacccgcgcg ctggccgcgc gtctcgccga
     2221 gggtcgcctg cccg
//
LOCUS       DAOQJH010000514         2232 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_37408_length_2232_cov_3.714286, whole genome shotgun sequence.
ACCESSION   DAOQJH010000514 DAOQJH010000000
VERSION     DAOQJH010000514.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2232)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2232
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37408_length_2232_cov_3.714286"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            210..1400
                     /locus_tag="PLQ18_11105"
     CDS             210..1400
                     /locus_tag="PLQ18_11105"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020655393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNE02242.1"
                     /translation="MRILTFSTLYPNATAPQFGVFVENRLRQLLASGEVEARVVAPVP
                     WVPGDYGWLGSYARLARIPAEEHRHGIAVRHPRYPQLPRIGETLHPLGLALGALPALR
                     RLQREGFDFELIDAHYFYPDGVAAALLARWLGKPFVVTARGTDLVTIAKHPLPQRMIR
                     AAAEAADGLITVSASLAGELAGLGIARERVTVLRNGVDLAHFRPAEREAARARFGVSG
                     PAPVLASVGHLIPRKGNEWILRALPALPGVRLLIAGQGPEQAMLEGLIRELGLAGRVT
                     LLGEVAHAELSQLYSAADVLVLATGHEGWPNVLLEAMACGTPVLANAVSGCPEIVTAP
                     EAGRLMPDRQPATIATHAAALLAALPDRAATRRYAEGFSWEATTRGQLDLFRAILARR
                     RAGG"
     gene            2147..2223
                     /locus_tag="PLQ18_11110"
     tRNA            2147..2223
                     /locus_tag="PLQ18_11110"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2181..2183,aa:Met,seq:cat)
BASE COUNT          299 a          812 c          798 g          323 t
ORIGIN      
        1 gacagggacg ggcgcgcgct cggaaggaac acggccttgt aatcgcgccg cggccccggc
       61 gttttagcgg acgcttaacc gcctcgcgcc gggcgctgcg gcgtcggatc ggtgcgggcc
      121 gcccgcggtt cccgtcgcgg cgggcgcgga tgatagccgt gcaccggccc gtatactccg
      181 cgcccttacg tcacggccgg tgtccgcgga tgcgcatcct cacgttctcg acgctctacc
      241 ccaacgccac ggcgccgcag ttcggcgtgt tcgtcgagaa ccgcctgcgc cagctgctcg
      301 cgagcggcga ggtcgaggcg cgcgtcgtcg cgccggtgcc gtgggtgccg ggtgactacg
      361 gctggctcgg gtcgtatgcg cggctcgcgc gcatcccggc cgaagaacac cgtcacggca
      421 tcgcggtgcg tcacccgcgc tatccgcagc tgccgcgcat cggcgaaacc ctgcatccgc
      481 tcgggctcgc gctcggcgcg ctgccggcgc tgcgccggct gcagcgcgag ggcttcgact
      541 tcgagctgat cgacgcgcac tacttctatc ccgacggcgt cgccgcggcg ctgctggcgc
      601 gctggctcgg caagcccttc gtggtcaccg cgcgcggcac cgatctggtc accattgcga
      661 agcacccgct gccgcagcgc atgatccgcg ccgccgccga agccgccgac ggcctgatca
      721 cggtctcggc ctcgctcgcc ggagaactcg ccggactcgg catcgcccgc gagcgcgtga
      781 cggtgctcag gaacggcgtc gatcttgcgc acttccggcc ggccgaacgc gaggccgcgc
      841 gcgcgcgctt cggcgtcagc ggcccggcgc cggtgctggc ctcggtcggg cacctgatcc
      901 cgcgcaaggg caacgaatgg atcctgcgcg cactgccggc cctgcccggc gtgcgcctgc
      961 tgatcgccgg gcagggaccg gagcaggcca tgctggaagg gctgatccgc gagcttggcc
     1021 tcgccggccg ggtcacgctg ctcggcgagg tggcgcacgc cgagctgtcg cagctctatt
     1081 ccgccgccga cgtgctggtg ctcgcgaccg gccacgaagg ctggccgaac gtgctgctcg
     1141 aagccatggc ctgcggcacg ccggtgctcg cgaacgccgt ctccggctgc ccggagatcg
     1201 tcaccgctcc ggaagccggc cggctgatgc cggaccgcca gcccgccacc atcgccacgc
     1261 acgccgctgc gctgctcgcc gcactgccgg atcgcgcggc gacgcgccgc tacgccgaag
     1321 gcttctcgtg ggaggccacg acgcgcggcc agctcgacct gttccgcgcg atccttgcgc
     1381 ggcgcagggc ggggggctga accggcatcg ggggcgattt gcactgctcc gatgccgcag
     1441 cggcgcggac gccggcagcc gcgccagacg ttttcccgtt ttcccgcagg cggcaggagc
     1501 tgaaaagacg gcgcgttcag ccgtgaccgc cctgagtggc accgtcatcg gcacaggccc
     1561 ggcccggacg ctgccggctg aacatccggc tgcgcgccgg cagtccgggc aggatgcggt
     1621 cgtctccaca aatgcacctg catccgcgcg ttcattgcgc gggcgatcag gcgccggtcg
     1681 cgcccgggta caggtgatga tttcgcgctc ggacggatcg ccgcgcaggg actgcatctt
     1741 ctggccggca ggctgatgcc gggtttcgcc gcatcggcgc cagtcagcgg ccggaaatcc
     1801 ccgcccagac ccgtcccctg cgagagcggc aggggtacag gtccggcagg gcgggggcgt
     1861 cggcaaaggc ggtcgactcc cgaccgcagg actggcacgg cggggccgtg gccggtggcc
     1921 gcagcagggc tgcgggcgtc ggcgacggtc ccgggccgga cgccgcgtga ctgtctgccg
     1981 atccggatgc tgcggcgatg accggcgtca gccgctgtgc ggctcgccag gcggccggaa
     2041 tacggttttc ggcccgaaac ttgccgttga cagccccgga ccccatccgt aatatgcgcc
     2101 gcctgttccg gccgtggcag cggcgggagc ggaaatcgat caaaaaggct acgtagctca
     2161 gctggttaga gcacagcact cataatgctg gggtcctcag ttcgattctg agcgtagcca
     2221 ccaaaaatca ag
//
LOCUS       DAOQJH010000515         2228 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_37531_length_2228_cov_3.430741, whole genome shotgun sequence.
ACCESSION   DAOQJH010000515 DAOQJH010000000
VERSION     DAOQJH010000515.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2228
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37531_length_2228_cov_3.430741"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1590
                     /locus_tag="PLQ18_11115"
     CDS             <1..1590
                     /locus_tag="PLQ18_11115"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNE02243.1"
                     /translation="FETISGYGAGELLGQTPRLLRSGHQDERFYAEMWSTLRRDGRWR
                     GELWNRRRNGDLYAQQLTIRAVPDPHGRSTRYLALASDVTEQRQRADRLQHLALHDAL
                     TGLPNRKLLNDRLAQAVRLGRRESLGFALIALDLDGFKGVNDTWGHAAGDRLLRSAGR
                     RLTNAVRASDTVARLGGDEFSVLLWDIAGRHDAEAVAAKLLAALRRSFSIDGHTVQIG
                     ASFGVALYPQDGADAATLTVHADAALYRAKSAGRNLYAFYTPTLEHEAHERNRLEGAL
                     RRLLDGGALAVAIQPEVDLADGRITGAELLARWPDADTEAPVPPARFIAVAEECGLIA
                     ALSEAVLQRALGLLRAWQPWLPRGFRLSVNLSPALLRQGEAQPLCRRVLAGEPALAAA
                     LMLDLPERGLFAEDAATRRRLHALRALGPGLALDDFGQGTLPLAGLREGDFDVLKITR
                     ALVAGLDAEAAQSGLAAALLGLGQALGLPVIAKGVETAAQARALRALGCTRALGFHFA
                     PPLTPEAFSGLLAGGHKLPPS"
     gene            complement(1605..>2228)
                     /locus_tag="PLQ18_11120"
     CDS             complement(1605..>2228)
                     /locus_tag="PLQ18_11120"
                     /inference="COORDINATES: protein motif:HMM:NF024687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HNE02244.1"
                     /translation="GSLADLPQARVQALTKELGVAGFDEILADIGLGTRMAPLIAKRL
                     LPGGESAEDRPRAGQGAPLLIKGTEGTVVSFGKCCRPLPGDAIIGYLTAGRGVVVHRE
                     NCRNVADYKKNPEKWIEIEWAESLRSDFPVDVRIDVSSKRGVFASVAAAISAADANIE
                     TISTSERDGVTATVNLTLNVRDRVHLAQVIKRLRALPEVLRIARRGQ"
BASE COUNT          276 a          829 c          797 g          326 t
ORIGIN      
        1 ttcgagacga tctccggcta cggcgccggc gaactgctcg ggcagacgcc gcggctgctg
       61 cgctccggcc atcaggacga acgcttttat gccgagatgt ggagcacgct gcgccgcgac
      121 ggccgctggc gcggcgagct ctggaaccgg cgccggaacg gtgacctgta cgcccagcag
      181 ctgacgatcc gcgccgtgcc cgatccgcac ggccgttcga cgcgctatct ggccctggcc
      241 tcggacgtga ccgagcagcg ccagcgcgcc gatcgcctgc agcacctggc gctgcacgat
      301 gcgctgaccg ggctgccgaa ccgcaagctg ctgaacgacc ggctggcgca ggcggtgcgc
      361 ctcggccggc gcgaatcgct cggtttcgcg ctgatcgcgc tcgatctcga tggcttcaag
      421 ggcgtcaacg atacctgggg ccacgccgcc ggcgaccgcc tgctgcgctc ggccggccgg
      481 cggctcacga acgccgtgcg cgcctccgac accgtcgcgc ggctcggcgg ggacgagttc
      541 tcggtgctgc tgtgggacat cgccggccgc cacgacgccg aggccgtcgc cgcgaagctg
      601 ctcgcggcgc tgcggcgcag tttctcgatc gacggccaca cggtgcagat cggcgccagc
      661 ttcggcgtcg cgctgtatcc gcaggacggc gccgatgccg cgacgctgac ggtgcatgcc
      721 gatgccgcgc tgtaccgcgc caagtccgcc ggccgcaacc tgtacgcgtt ctacacgccg
      781 acgctggagc acgaggcgca cgagcgcaac cggctcgaag gggcgctgcg ccgcctgctc
      841 gatggcggtg cgctggcggt cgccatccag ccggaggtcg atctcgccga tggccgcatc
      901 accggcgccg aactgctcgc ccgctggccc gacgctgaca ccgaagcgcc ggtgcccccg
      961 gcgcgcttca ttgcggtggc cgaggaatgc gggctgatcg ccgcgctcag cgaagccgtg
     1021 ctgcagcgcg ccctcggact gctccgcgcc tggcagccgt ggctgccgcg cgggtttcgc
     1081 ctgagcgtca acctgtcgcc ggcgctgctg cgtcagggcg aggcgcagcc cctgtgccgg
     1141 cgcgtgctcg ccggcgagcc ggcgctggcc gcggcgctga tgctcgatct gcccgaacgc
     1201 ggcctgttcg ccgaggacgc cgcgacccgg cgccggctgc acgcgctgcg cgcgctgggc
     1261 cccggactcg cgctcgatga cttcggtcag ggcacgctgc cgctcgcggg cctgcgcgaa
     1321 ggtgacttcg acgtgctgaa gatcacgcgg gcgctggtgg ccggactcga tgcggaggct
     1381 gcgcagtcgg ggctggcggc cgccctgctc ggccttggtc aggccctcgg cctgccggtg
     1441 atcgccaagg gcgtcgagac cgcagcgcag gcccgcgcgc tgcgcgcgct gggctgcacg
     1501 cgggccctcg gctttcactt cgctccgccc ctgacgccgg aggcgttctc cggactgctc
     1561 gccggcggac acaagctgcc accctcctga gcggcgcgcg ccgctcactg gccgcggcgg
     1621 gcgatcctca gcacttccgg cagcgcgcgc aggcgcttga tgacctgcgc aagatgcacg
     1681 cggtcgcgca cgttcagcgt caggttgacg gtcgccgtga cgccgtcgcg ctcgctggtc
     1741 gagatggtct cgatgttggc atcggccgcc gagatcgccg cggcgacgct cgcgaacacg
     1801 ccgcgcttgc tggagacatc gatgcgcacg tcgaccggga aatcactgcg caacgactcg
     1861 gcccactcga tctcgatcca cttttccgga ttcttcttgt agtcggcgac gttgcggcag
     1921 ttctcgcgat gcacgaccac gccgcgcccg gcggtcaggt agccgatgat cgcgtccccg
     1981 ggcagcggcc ggcagcactt gccgaagctg accaccgtgc cctcggtgcc cttgatcagc
     2041 agcggcgcgc cctggccggc acgcgggcgg tcctcggccg actcgccgcc gggcagcagg
     2101 cgcttggcga tcagcggcgc catgcgcgtg ccgaggccga tgtcggcgag gatctcgtcg
     2161 aagcccgcga cgccgagttc ctttgtcagc gcctgcacgc gcgcctgcgg caggtcggcc
     2221 aggctgcc
//
LOCUS       DAOQJH010000516         2225 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_37604_length_2225_cov_6.954839, whole genome shotgun sequence.
ACCESSION   DAOQJH010000516 DAOQJH010000000
VERSION     DAOQJH010000516.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2225)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2225)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2225
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37604_length_2225_cov_6.954839"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(212..574)
                     /locus_tag="PLQ18_11125"
     CDS             complement(212..574)
                     /locus_tag="PLQ18_11125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008930784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HNE02245.1"
                     /translation="MSIRPTDLCLHQQTRILEVRFEDGAHFEMSCEYLRVFSPSAEVR
                     GHGPGQETLQVGKEEVEIVDIVPVGNYAVKLVFSDGHDSGLYSWNVLYELGRDQAANW
                     QQYLARLAAAGHVRRAGP"
     gene            666..1190
                     /locus_tag="PLQ18_11130"
     CDS             666..1190
                     /locus_tag="PLQ18_11130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02246.1"
                     /translation="MSLQGDNLHLQHAGGAECRPRPVVVAAKDRCITRMQAESDRYVA
                     FLRRYVIDLTCHCRKSVRGIDSTSEQPSDDILKKQPLAVRMPRGTKVAFHPVERSWRR
                     SADPKRGSAGPVVAPGAILPDPDVQHPEVLVMTNYISLRAVWHALKERGYWRMCAAMS
                     TDHPVPVSPNRRCL"
     gene            complement(1480..>2225)
                     /locus_tag="PLQ18_11135"
     CDS             complement(1480..>2225)
                     /locus_tag="PLQ18_11135"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNE02247.1"
                     /translation="QVESLTQFIRVQNSRRAVGLKTPALSLHLIFTGNPGTGKTTVAR
                     RIGSIYRRLGLLQKGHVIECQRQDLVGEYIGHTAPKVRRKCDAAMDGVLFIDEAYSLM
                     QPNSDKDFGSEAIAALVAEMENRRDRLVVIVAGHTQEMKAFLDANPGLRSRFWRTLHF
                     EDYTAGELLEIFERFAIDGDYRLDAQARSVLLGRFEKSLAGRTRTFGNARHARNLFEQ
                     ALEKHAARVGRLADPDCAALQTITAFDLP"
BASE COUNT          359 a          739 c          737 g          390 t
ORIGIN      
        1 tgctggtagc cgaaatgagt ggtggcggtg tcgtcgttac cgggcgtttc cgggctggcc
       61 atctcgcgtg ctccgtcggg ccggccgggc gaccgggcct gcgacctgga aggattggga
      121 atgcgggtcc tgatgcgggg ctgccgcttt tgcgctgcag caggcacggc cctgcagcat
      181 agccacgcgg ccccgccggc tcaaaccgcc gtcagggccc ggcgcggcgc acgtgaccgg
      241 cggcagcgag gcgtgcgagg tactgctgcc agttcgcggc ctggtcgcga ccgagttcgt
      301 acagcacgtt ccacgaatac aggcccgaat cgtggccgtc ggaaaacacc agcttcaccg
      361 catagttgcc gaccggcacg atgtcgacga tctcgacctc ctccttgccg acctgcaggg
      421 tttcctgccc cggcccgtgg ccgcgcacct cggccgacgg gctgaacacc cgcaggtatt
      481 cgcagctcat ctcgaaatgc gcgccgtcct cgaagcggac ttcgaggatg cgcgtctgct
      541 ggtgcaggca cagatcggtc gggcggatgc tcatggcggc gctcccggcg gaggcgggtg
      601 aagacggagg cgcccactgt gccacggcag gcgccgatcg gggcaggcac ggattcgtaa
      661 cagaggtgtc gctgcaaggc gacaatttgc acctgcagca tgcaggaggt gcagaatgcc
      721 ggccacggcc ggtcgtggtg gccgcgaagg accgatgcat cacgcggatg caggcagaat
      781 cagacagata cgtcgctttt ttacgtcgct atgtcatcga cttgacgtgt cactgtcgca
      841 aaagcgtcag gggaattgac agtacatcag aacagccatc ggacgacatt ttgaaaaaac
      901 aaccacttgc cgtgcgaatg cctcgtggca ccaaagttgc ttttcatcca gtcgagcgtt
      961 cctggcgtcg ttccgccgac ccgaaacgcg gttccgcagg ccccgtggtc gcccctggcg
     1021 ccatcctgcc ggatccggat gttcagcacc ctgaggtgct cgtcatgacc aactacatct
     1081 cgttacgcgc cgtctggcat gccctgaaag agcgcggcta ctggcgcatg tgcgccgcga
     1141 tgtccaccga ccatccggtc ccggtcagcc cgaaccgccg ctgcctgtag cggcagcccc
     1201 gtcccgatcc cgcagttcct caccctccgg tcgaccggcc gccgcccgct gcggcaaccc
     1261 ggcccgacct gccgctccgg cccgatctgc tggccgggca acgcgccgcc ggcggcgcgt
     1321 gcgaggcggc accccggcgc ccgcagtcgc gccggatttc cagcacgtcc ggtgaagcgc
     1381 cgatcggcag catcctcgca tccctgctga agaccacggc tgtcgctggt gtcggtgcgc
     1441 aagcgtccgc gaagggcggt gcgtctccgc aggcgcggtt cagggcagat cgaaggccgt
     1501 gatcgtctgc agggctgcgc agtcgggatc ggcgaggcgt cccacccgcg ccgcgtgttt
     1561 ttccagcgcc tgctcgaaca ggttgcgggc gtgacgggcg ttgccgaagg tgcgcgtcct
     1621 gccggccagc gatttttcga acctgccgag cagcaccgac cgtgcctgcg cgtcgaggcg
     1681 gtaatccccg tcgatggcga agcgctcgaa gatttcgagc agctcgccgg ccgtatagtc
     1741 ctcgaagtgc agcgtgcgcc agaagcggga gcgcaggccg ggattggcat cgaggaatgc
     1801 tttcatctcc tgcgtatggc cggccacgat cacgaccagc cggtcccggc ggttttccat
     1861 ttcggcaacg agcgccgcaa tcgcttcgct gccgaagtcc ttgtcactgt tcggctgcat
     1921 cagcgaatac gcttcgtcga tgaacagcac gccgtccatg gccgcatcgc acttgcgtct
     1981 gaccttcggg gcggtatggc cgatgtattc gccgaccaga tcctggcgct ggcattcgat
     2041 gacgtggcct ttctgcagca ggcccagtcg ccggtaaatg ctgccgatcc gccgggcgac
     2101 cgtggtcttg ccggtgccgg gattgccggt gaagatcaga tgcagcgaca aagccggcgt
     2161 tttcaggccg acggcgcgcc gggagttctg gacgcggatg aactgggtca gcgattccac
     2221 ctgtg
//
LOCUS       DAOQJH010000517         2220 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_37759_length_2220_cov_5.064665, whole genome shotgun sequence.
ACCESSION   DAOQJH010000517 DAOQJH010000000
VERSION     DAOQJH010000517.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2220
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37759_length_2220_cov_5.064665"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..205)
                     /locus_tag="PLQ18_11140"
     CDS             complement(<1..205)
                     /locus_tag="PLQ18_11140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005959169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pili methyl-accepting chemotaxis
                     transducer N-terminal domain-containing protein"
                     /protein_id="HNE02248.1"
                     /translation="MTLPPLPPLRDSLLWRMGLLLGGIALLALIGMSNALLTAVASAG
                     DAAAINIAGSLRMQSYRIATRLGA"
     gene            649..>2220
                     /locus_tag="PLQ18_11145"
     CDS             649..>2220
                     /locus_tag="PLQ18_11145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020088036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNE02249.1"
                     /translation="MSVGKPQQVRATVASTLAFTVCFAVWTIFSIIGIQIKKQLGLSE
                     TEFGLLVGTPILTGSLIRLVLGVWTDQIGGRVVFLWVMLSAAIATWLLTYAQSYQMFL
                     VAALGVGIAGGSFAVGIAYVSKFFDTRSQGTALGVFGAGNVGAALTKFVAPFVMVAYG
                     WQTVAQVWAAAIAITGVLFYLCTEDDPSLKARRATGTKPQSFLQQMEPLKHLQVWRFS
                     LYYFFVFGAFVALALWLPRYLTGAYGMDIKEAGMVAAMYSIPASLFRIVGGWLSDRYG
                     ARTVMYWTFIVSAICTLILSYPPADFVVRGIHGPISFHLSTGVGGFIAIAFVLGFFMS
                     LGKAAVYKHIPVYYPQHVGSVGGVVGMIGGLGGFLLPITFGMLNDLTGIWQSCFMLLF
                     AIVTVSLLWMHFSILRMERRVVPQLKDLPQDLPELASESPMTLTEWSPEDKDFWETKG
                     RRIAQRNLWISIPNLLLAFAVWMVWSVVVVNLPNLGFKYSANQLFWLAALPALSGATL
                     RIFYSFMVPIFGGRRW"
BASE COUNT          325 a          749 c          729 g          417 t
ORIGIN      
        1 cggcaccgag ccgggtcgcg atccggtagc tctgcatgcg cagcgagccg gcgatgttga
       61 tcgccgcggc gtcgccggca ctggcgaccg cggtcagcag cgcgttgctc atgccgatca
      121 gcgcgagcag cgcgatgccg ccgagcagca ggcccatgcg ccagagcagg gaatcgcgca
      181 gcggcggcag cgggggcagg gtcatcagcg ggggtctctc gcaggaaatc cggtcggtgg
      241 tcgaaggggc cggcttgcgg actgcacagc ctcgtcaggg cccggagccg cgaccttgat
      301 gccggtcaag gcgcaccggc cggctccggc actgccacct tcgctctcac ccgcccgcgc
      361 gggcggggta tccggcggca ggacggggta gccggaaccg gcaccgcctg cgtccgcccg
      421 ggcgcgattc cggcaccacg ggcctgcatt gatagcgcat tcaggcactt gccgcctgag
      481 cctgttttac tccctacggg gcagcgcgcg tggcaattca gcctaccccc accggaagcc
      541 attgattcac atcaagcagt cacccgccgc ccgacaccga cgatggcgct cagtccggat
      601 tccacgatcc gggaaacgct ctgatccggt catccgaggt atctgcatat gtcggtcgga
      661 aaaccccaac aggtgcgggc gacggtggct tccacccttg ccttcaccgt ctgcttcgcg
      721 gtctggacga tcttctcgat catcggcatc cagatcaaaa aacaactcgg cctcagcgag
      781 accgaattcg gcctgctggt cggcacgccg atcctgaccg gctcgctgat ccggctcgtg
      841 ctcggcgtct ggaccgacca gatcggcgga cgcgtcgtgt tcctgtgggt gatgctgtcg
      901 gcggcgatcg caacctggct gctgacctac gcgcagagct accagatgtt cctggtcgcc
      961 gcgctcggcg tcggcatcgc cggcggctcg ttcgcggtcg gcatcgccta cgtgtcgaag
     1021 ttcttcgata cccgctcgca gggcacggcg ctcggcgtgt tcggtgccgg caacgtcggc
     1081 gcggcgctga ccaagttcgt cgcgccgttc gtgatggtcg cctacggctg gcagaccgtc
     1141 gcgcaggtct gggcggccgc gatcgcgatc accggcgtgc tgttctatct ctgcaccgag
     1201 gatgatccaa gcctgaaggc ccgacgcgcg accggcacga agccgcagag cttcctgcag
     1261 cagatggagc cactgaagca cctgcaggtg tggcgcttct cgctgtacta ctttttcgtg
     1321 ttcggtgcct tcgtggcgct ggcgctgtgg ctgccgcgct atctgaccgg cgcctacggc
     1381 atggacatca aggaagccgg catggtcgcg gcgatgtact cgatcccggc ctcgctgttc
     1441 cgcatcgtcg gcggctggct gtcggaccgg tacggtgcgc gcacggtgat gtactggacc
     1501 ttcatcgttt cagcgatctg cacgctgatc ctgtcgtacc cgccggccga tttcgtcgtc
     1561 aggggcatcc acggcccgat cagcttccac ctgtccaccg gcgtcggcgg cttcatcgca
     1621 atcgccttcg tgctcggttt cttcatgtcg ctcggcaagg cggcggtcta caaacacatc
     1681 ccggtctact acccgcagca cgtcggctcg gtcggcggcg tggtcggcat gatcggcggc
     1741 ctcggcggct tcctgctgcc gatcaccttc ggcatgctca acgacctgac cggcatctgg
     1801 cagagctgct tcatgctgct gttcgcgatc gtcaccgtgt cgctgctgtg gatgcacttc
     1861 tcgatcctgc gcatggaacg gcgcgtcgtg ccgcagctga aggatctgcc gcaggacctg
     1921 ccggagctgg cctcggaaag cccgatgacg ctgaccgagt ggagccccga ggacaaggat
     1981 ttctgggaaa cgaagggccg ccggatcgcg cagcgcaatc tgtggatctc gatcccgaac
     2041 ctgctgctcg cgttcgcggt gtggatggtc tggtcggtcg tggtcgtgaa cctgccgaac
     2101 ctcggcttca agtactcggc caaccagctg ttctggctcg ccgcgctgcc ggcgctgtcg
     2161 ggcgcgacgc tgcgcatctt ctacagcttc atggtgccga tcttcggcgg ccggcgctgg
//
LOCUS       DAOQJH010000518         2218 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_37840_length_2218_cov_3.999075, whole genome shotgun sequence.
ACCESSION   DAOQJH010000518 DAOQJH010000000
VERSION     DAOQJH010000518.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2218)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37840_length_2218_cov_3.999075"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1355
                     /locus_tag="PLQ18_11150"
     CDS             <1..1355
                     /locus_tag="PLQ18_11150"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HNE02250.1"
                     /translation="GDGAVEPFAHTLLITLASGLLIWLPVRRQKLDLRNRDGFLVVVA
                     FWVTLSLLSAIPFMLSEQPHMPLVDAVFEAVSGLTTTGATVLAGIDSLPHAIVYYRAQ
                     LNFFGGMGIVVLAVALLPMLGVGGMQIYRAETPGPMKDEKLTPRITETAKNLWYIYVG
                     LNVLCTAAFWLAGMDLFDSVCHSFATIALGGFSTHDASIGFYQSDAIEFVAGFFSLVA
                     AVNFALFFFAWRQKSLRAILVDEEFRFFVKVMAVLIGAAIAGLYFSGTFPLWESIVHG
                     FFQTASIVTDNGLVAAGYPDWPMAVTLLLLLGSFFGGCVGSTCGGIKAIRFLLLYRQS
                     VREMKLLIRPTAQIPVRLGTRTVPDRVMQAVLAFYFLYIFAYCACSIGVAATGADLVT
                     AFGSVAGCINNMGVGLGETASNFSPLSDTATWIVTASMLIGRLEVFPLLLLFVPSFWR
                     "
     gene            1484..2077
                     /locus_tag="PLQ18_11155"
     CDS             1484..2077
                     /locus_tag="PLQ18_11155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02251.1"
                     /translation="MLQRLFGVPVLSVLLWLAAAPAQATAVIEDPAAGGHSVFVWEAA
                     GALSGIFDAGPDDGVSSEAGSTDWQLALAAPGLLGIVISGVYNRPPTVLDLLIDGIGV
                     PWTTSSVRAEVISEDDQGVPLEIIHHFDARLDGLLLAAGEHRLTLLPSADNTDGTAGF
                     ITFAAIVPVPAPPALALLATGFGLVVALRARRTAKAA"
BASE COUNT          297 a          798 c          721 g          402 t
ORIGIN      
        1 acggcgacgg cgcggtcgag ccgttcgcac acacgctgct gatcacgctc gcgagcggcc
       61 tgctgatctg gctgccggtg cgtcgccaga agctcgacct gcgcaaccgc gacggctttc
      121 tggtcgtcgt cgcgttctgg gtgacgctgt cgctgctgtc ggcgatcccg ttcatgctct
      181 ccgagcagcc gcacatgccg ctggtcgatg cggtgttcga ggccgtctcg gggctgacca
      241 cgaccggcgc caccgtgctc gccggcatcg acagcctgcc gcacgcgatc gtgtattacc
      301 gcgcgcagct caacttcttc ggcggcatgg gcatcgtcgt gctggcggtg gcgctgctgc
      361 cgatgctcgg ggtcggcggc atgcagatct accgcgccga gacgcccggc ccgatgaagg
      421 acgaaaagct cacgccgcgc atcaccgaga ccgccaagaa cctctggtac atctacgtcg
      481 gcctgaacgt gctctgcacc gccgcgttct ggctggccgg catggacctc ttcgattcgg
      541 tctgccacag cttcgcgacc atcgcgctcg gcggcttctc gacgcacgac gcctcgatcg
      601 gcttctatca gagcgacgcc atcgagttcg tcgccggctt cttctcgctg gtcgcggccg
      661 tcaacttcgc gctgttcttc ttcgcgtggc gccagaagag cctgcgcgcg atcctcgtcg
      721 acgaggagtt ccgcttcttc gtcaaggtca tggccgtgct gatcggcgcg gcgatcgccg
      781 ggctgtactt cagcggcacc ttcccgctgt gggagtcgat cgtgcacggc ttcttccaga
      841 ccgcctcgat cgtcaccgac aacggcctgg tcgccgccgg ctatccggac tggccgatgg
      901 cggtgacgct gctgctgctg ctcggcagct tcttcggcgg ctgcgtcggc tcgacctgcg
      961 gcggcatcaa ggcgatccgc ttcctgctgc tgtaccgcca gagcgtgcgc gagatgaagc
     1021 tgctgatccg cccgaccgcg cagatcccgg tgcgcctcgg cacgcgcacg gtgccggatc
     1081 gcgtcatgca ggcggtgctg gcgttctact tcctgtacat cttcgcgtac tgcgcgtgtt
     1141 cgatcggcgt cgccgcaacc ggcgccgacc tcgtcaccgc cttcggctcg gtcgccggct
     1201 gcatcaacaa catgggcgtc ggcctcggcg agacggcctc gaacttctcg ccgctcagcg
     1261 acaccgcgac ctggatcgtc accgcctcga tgctgatcgg ccggctggag gtgtttccgc
     1321 tgctgctgct gttcgtgccg tcgttctggc gctgagcagg cgttgccgga aataccctcc
     1381 ctccggacaa gaccgcctga ttccggccgc cctccatcgc gcgggtccac gccggctaca
     1441 ctcaccggcc acgacgggca ccggagatgg ccggaggtgc gacatgctgc agcgtctttt
     1501 cggcgtcccg gtactgagcg tgctgctgtg gctcgcggcc gctccggcgc aggcgacggc
     1561 tgtgatcgag gatcccgccg ccggcggtca cagtgtgttt gtctgggaag cagccggtgc
     1621 cctctcgggc atcttcgatg cgggtccgga cgacggcgtc tccagcgaag ccggcagcac
     1681 cgactggcag ctcgcccttg cggcgccggg cctgctcggg atcgtcatca gcggcgtcta
     1741 caaccgtccg ccgacggtgc tcgatctgct gatcgacggc atcggcgtac cctggaccac
     1801 ctccagcgtc cgcgccgagg tgatcagcga ggatgatcag ggcgtaccgc tggagatcat
     1861 ccaccacttc gatgcccggc tcgatggcct gctgctggcg gccggggaac accggctgac
     1921 gctgctgccc tcggccgaca acaccgacgg cacggcaggt ttcatcacct ttgccgccat
     1981 cgtgccggta ccggctcccc cggcactggc cttgctggcg accggtttcg ggcttgtcgt
     2041 ggccttgcgg gcacgtcgga ccgccaaagc agcctgaagc gcaggggcat atcccttccc
     2101 tgcgcaggcg gtaacgacgc ccgggcagcg cggccgtcgc cgcggcgcga agccccggcg
     2161 gcctcacccg aagccgcgca gcgccagccc gatctgcagc agcccgaaca gcacgacc
//
LOCUS       DAOQJH010000519         2215 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_37944_length_2215_cov_3.553704, whole genome shotgun sequence.
ACCESSION   DAOQJH010000519 DAOQJH010000000
VERSION     DAOQJH010000519.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2215)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2215)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2215
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37944_length_2215_cov_3.553704"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(126..1697)
                     /gene="purH"
                     /locus_tag="PLQ18_11160"
     CDS             complement(126..1697)
                     /gene="purH"
                     /locus_tag="PLQ18_11160"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017641062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HNE02252.1"
                     /translation="MTDQPIRIRRALVSVSDKTGIVDFARELAGFGVEILSTGGTAKL
                     LAESGVPVVEVSDYTGFPEMMDGRVKTLHPKVHGGLLGRRGVDEDVMAEHGIPPIDMV
                     VVNLYPFAQTVAKPGCTLEDAIENIDIGGPTMLRSAAKNHAGVTVVVDAADYGRVLDE
                     LKAQDGAIGHALRFDLAVKTFEHTARYDGMIANYLGAMIEHGRPEGLPRTFNVQFRKA
                     QEMRYGENPHQHAAFYVGHHPEPGSISSARQLQGKELSYNNIADTDAALECVKQFDAP
                     ACVIVKHANPCGVAVGATILEAYERAYATDPESAFGGIIAFNRELDAATAQAIVGRQF
                     VEVIIAPAVSAEALAVTQAKQNVRVLDCGAWTVTAVPRLDYKRVTGGLLVQDADLELH
                     GELKIVTQRAPSDAEMQDLLFTWRVAKFVKSNAIVYGKDNATIGVGAGQMSRINSARI
                     AAIKAEHAGLPVAGSVMASDAFFPFRDGLDAAAKAGIRAVIQPGGSMRDAEVIAAADE
                     HGMAMVFTGMRHFRH"
     gene            1878..>2215
                     /locus_tag="PLQ18_11165"
     CDS             1878..>2215
                     /locus_tag="PLQ18_11165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004334173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HNE02253.1"
                     /translation="MPPVPAEPRADAGTPLALRCLLAVRLPFLTVTLFAYLIGLGTAV
                     RDGHALPAASLAGLLLALICHAAVNVINDWADARNGSDAANTERIYPFTGGTRVIQNG
                     VLGERAMLVLA"
BASE COUNT          319 a          738 c          790 g          368 t
ORIGIN      
        1 agccgctcga tcgtcagatc ctcgaacagc cggaaattcc ggatcgtcag cgaatcgagc
       61 atcggggcgg tcctcgtcgg ggcggcgtcg cggtctagcg taggccgcga cgccccggcc
      121 ggtcatcagt ggcggaagtg gcgcatgccg gtgaacacca tggccatgcc gtgctcgtcg
      181 gcggcggcga tgacttcggc gtcgcgcatc gagccgccgg gctggatcac ggcgcggatg
      241 ccggccttcg cggccgcatc gaggccgtcg cggaacggga agaaggcgtc ggaggccatc
      301 accgagccgg cgaccggcag gccggcgtgc tcggccttga tcgcggcgat gcgcgcgctg
      361 ttgatgcggc tcatctggcc ggcaccgacg ccgatcgtgg cgttgtcctt gccgtagacg
      421 atggcattgg acttcacgaa cttcgcgacc cgccacgtga acagcagatc ctgcatctcg
      481 gcgtcgctcg gtgcgcgctg cgtgacgatc ttcagctcgc cgtgcagttc gaggtcggca
      541 tcctgcacca gcaggccgcc ggtcacgcgc ttgtagtcga ggcgcggcac ggccgtgacc
      601 gtccacgcgc cgcagtcgag cacgcgcacg ttctgcttcg cctgcgtgac cgccagcgct
      661 tcggcggaca cggccggcgc gatgatgacc tcgacgaact ggcggccgac gatggcctgc
      721 gcggtcgcgg cgtcgagttc gcggttgaag gcgatgatgc cgccgaacgc cgattcgggg
      781 tcggtcgcat acgcgcgttc gtaggcttcg aggatcgtcg ctccgaccgc gacgccgcag
      841 ggattggcgt gcttgacgat gacgcaggcc ggcgcgtcga actgcttcac gcattcgagc
      901 gcggcatcgg tgtcggcgat gttgttgtac gagagttcct tgccctgcag ctggcgcgcc
      961 gagctgatcg aacccggctc cgggtggtgg ccgacataga aggcagcgtg ctggtgcggg
     1021 ttctcgccgt agcgcatctc ctgcgccttg cggaactgca cgttgaaggt gcgcggcaag
     1081 ccttcggggc ggccgtgctc gatcatcgcg ccgagatagt tggcgatcat gccgtcgtag
     1141 cgcgcggtgt gctcgaaggt cttgaccgcg aggtcgaagc gcagcgcgtg gccgatcgcg
     1201 ccgtcctggg ccttgagttc atcgagcacg cggccgtagt cggcggcatc gacgacgacg
     1261 gtgacgccgg cgtggttctt ggccgccgag cgcagcatgg tcgggccgcc gatgtcgatg
     1321 ttctcgatgg cgtcctccag cgtgcagccg ggtttcgcga ccgtctgtgc gaacgggtag
     1381 aggttgacga ccaccatgtc gatcggcggg atgccgtgct cggccatcac gtcctcatcg
     1441 acgccgcgcc ggccgagcag gccgccgtgc accttcgggt gcagcgtctt gacgcggccg
     1501 tccatcatct ccgggaagcc ggtgtagtcg gagacctcga ccaccggcac gccggattcg
     1561 gcgagcagct tcgcggtgcc gccggtggag aggatttcga cgccgaaacc ggcgagttcg
     1621 cgggcgaagt cgacgatacc ggtcttgtcg gagacgctga cgagggcgcg gcggatgcgg
     1681 atgggctgat cggtcatgag ggcgtaagcc ggcggctgtg aaaaaaggcg cgcagtatag
     1741 cgatcctgac gcggaagggt ctgatccggc gtgcaaatgc cggaaaccag cgggaaaccg
     1801 cggtgtacgc ccccgtgaac cggcaccggg ctcccgtaca ctggcgcgtt ccgcccccgc
     1861 ctcccggatg ccccccgatg ccgcccgttc ccgccgaacc ccgtgccgac gccggcacgc
     1921 cgctcgcgct gcgctgcctg ctggcggtgc gcctgccatt cctgacggtg acgctgtttg
     1981 cgtacctgat cggcctcggc accgccgtgc gcgacggcca tgccctgccc gcagccagtc
     2041 tggccgggct gctgctggcc ctgatctgcc acgccgccgt caacgtcatc aacgactggg
     2101 ccgatgcgcg caacggcagc gatgccgcca acaccgagcg catctatccg ttcaccggcg
     2161 gtacgcgcgt gatccagaac ggcgtgctcg gcgagcgcgc gatgctcgtg ctggc
//
LOCUS       DAOQJH010000520         2214 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_37987_length_2214_cov_2.638722, whole genome shotgun sequence.
ACCESSION   DAOQJH010000520 DAOQJH010000000
VERSION     DAOQJH010000520.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2214)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2214)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2214
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37987_length_2214_cov_2.638722"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(246..623)
                     /locus_tag="PLQ18_11170"
     CDS             complement(246..623)
                     /locus_tag="PLQ18_11170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007506041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family detoxifying hydrolase"
                     /protein_id="HNE02254.1"
                     /translation="MQIIATSEAPAAIGPYSQGIVVNGLLYTSGQIPLNAAGEFVEGE
                     IDVQTEQDIDNLAAILAAAGATLADVVKTTVFVTDLNDFARLNEIYGQRFGEHRPARS
                     TVQVARLPRGAKVEIEAVVKLPG"
     gene            complement(735..1067)
                     /locus_tag="PLQ18_11175"
     CDS             complement(735..1067)
                     /locus_tag="PLQ18_11175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02255.1"
                     /translation="MNQAEATSTGTGLDHPAAIAALTAYATMERSKSAHFEMLRALEE
                     KYQKYGRPSADEEAELAGRLAGHDRAVRDFREAVAALRAADETAFAAVMAELHSEASR
                     ATGTPTEQ"
     gene            1161..>2214
                     /locus_tag="PLQ18_11180"
     CDS             1161..>2214
                     /locus_tag="PLQ18_11180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HNE02256.1"
                     /translation="MTFRTAPAIAEARALLALAVPLMLAQLAQMLMGYTDTLMVARVG
                     ALELAAVGTGLALWHPLYLAVTGLLMAESALIARLAGAGRADAAGGLRRHARALALAC
                     ALPAVVLMNHVGPLLGALGVDAAIVPVAQDYLGALSFGVPGAFWYLAERYAIEGLGRT
                     RPVLVAGLAAFVLNIGLNWLLIYGHLGLPALGARGCGYATAACFWLEACGLAWLNRRL
                     PALRSHSPSPDAPGLRALLGLGLPIAAAVLFEVGIFCAVALALGRLGPVAVAAHQIAL
                     TTASLTFMIPLALAAAITIRIGGALGAGDVPAARRAGWTGIALAAAFMALSATGIGFG
                     APWIAALYTGDAAVAAL"
BASE COUNT          242 a          758 c          840 g          374 t
ORIGIN      
        1 gcttgagacg ggttgcgacg gaacctagca gtggatgtcg cgatgacaac gcgccgggca
       61 ttaaggatcg gaaaggattg ctgtccggcc ggcgaccggc ttcgggtcgc cggtgcctga
      121 ggaggcgtgt ccggcaagca gcagggtgcg cggaatgacc cgctgccggt gcaacggctc
      181 ccggcgttgc gggccgtggt tccgggagtg gaggcgatgc cggcatggac ccgccggccg
      241 ccgtctcagc ccggcagctt cacgaccgcc tcgatctcga ccttcgcgcc gcgcggcagg
      301 cgggcgacct gcacggtcga gcgtgccggg cgatgctcgc cgaagcgctg gccgtagatt
      361 tcgttgaggc gtgcgaagtc gttcaaatcg gtcacgaaca ccgtggtctt gaccacgtcg
      421 gcgagcgtcg cgccggcggc ggcgaggatc gcggcgaggt tgtcgatgtc ctgctcggtc
      481 tgcacgtcga tctcgccttc gacgaactcg ccggcggcgt tcagcggaat ctggccggag
      541 gtgtacagca ggccgttgac gacgatgccc tgcgaatacg ggccgatcgc ggcgggggct
      601 tcggacgtgg cgatgatctg catgaggcat tcggctccgg ttcgggaacg gatgccggcc
      661 ggaggcgtgc cggccggggc gggatggcca tgctggccgc ggggacttgc cggcgcaagc
      721 aggtcgggtc ggcttcactg ctcggtcggg gtgccggtcg cgcgcgaggc ctcgctgtgc
      781 agttcggcca tcaccgcggc aaacgctgtc tcgtcggctg cgcgcagcgc cgcgaccgcc
      841 tcgcggaagt cgcgcacggc acggtcatgc ccggcgagcc gtccggcgag ttcggcttcc
      901 tcgtcggccg agggccggcc gtacttctga tacttctcct ccagcgcccg cagcatctcg
      961 aaatgcgcgc tcttgctgcg ctccatcgtg gcgtaggcgg tcagcgcggc gatggcggcc
     1021 gggtggtcga gtccggtgcc ggtgctggtg gcttcggctt ggttcatgat gcgcggctcc
     1081 tgcggacggc atgtttcaga tgctccgcga tgatgggggg atcaggcggc ggatacgcgg
     1141 ccgccgccgg gctgcgcatg atgacattcc gcaccgcgcc tgccatcgcc gaagcgcgcg
     1201 cgctgctcgc actcgccgtg ccgctgatgc tcgcgcagct cgcgcagatg ctgatgggct
     1261 acaccgacac gctgatggtc gcgcgcgtcg gcgcgctcga acttgccgcg gtcggcaccg
     1321 ggctcgcgct ctggcatccg ctgtacctgg ccgtcaccgg cctgctgatg gccgagagcg
     1381 cgctgatcgc ccgcctcgcc ggcgccggcc gtgccgatgc agccggcggg ctgcgccggc
     1441 atgcgcgcgc gctggcgctt gcctgcgcgc tgccggccgt cgtcctgatg aatcacgtcg
     1501 ggccgctgct gggcgcgctc ggcgtcgatg ccgcgatcgt gccggtcgcg caggactatc
     1561 tcggagccct gtccttcggc gtgccggggg cgttctggta tctggccgag cgttacgcga
     1621 tcgaaggcct cggccgcaca cggccggtgc tggtcgcggg cctcgcggcg ttcgtgctga
     1681 acatcggcct caactggctg ctgatctacg gccatctcgg cctgccggcg ctcggggcgc
     1741 gcggctgcgg ctatgcgacc gccgcctgct tctggctgga agcctgcggg ctcgcgtggc
     1801 tgaaccgccg gctgccggcg ctgcgcagcc acagtccttc gccggatgcc cccggcctgc
     1861 gtgcgctgct cgggctcggc ctgccgatcg cggcggcggt gctgttcgag gtcggcatct
     1921 tctgcgccgt cgcgctcgcg ctcggccggc tcgggccggt tgcggtcgcc gcgcaccaga
     1981 tcgcgctgac caccgcgagc ctgaccttca tgattccgct ggcgctggcg gcggcgatca
     2041 cgatccgcat cggcggtgcg ctcggggccg gcgacgtgcc ggcggcgcgc cgggccggct
     2101 ggactggcat cgcgctcgcc gcggcgttca tggcgctgtc ggcgaccggc atcggcttcg
     2161 gtgcgccgtg gatcgcggcg ctctacaccg gcgatgccgc ggtcgcggcg ctgg
//
LOCUS       DAOQJH010000521         2211 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_38063_length_2211_cov_4.256957, whole genome shotgun sequence.
ACCESSION   DAOQJH010000521 DAOQJH010000000
VERSION     DAOQJH010000521.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2211)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2211)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2211
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38063_length_2211_cov_4.256957"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..672
                     /locus_tag="PLQ18_11185"
     CDS             <1..672
                     /locus_tag="PLQ18_11185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4239 domain-containing protein"
                     /protein_id="HNE02257.1"
                     /translation="ETERERVVLNTIETAIYALLGLMIAFTFSGAAERFDARRSLTVQ
                     EGNAIGTAYLRLDLLPAAVQPALREKFRRYTEARIAVYQALPDIEASNTHAARAGALQ
                     GQIWAEAVAALKDAPPASSLLLLPALNDMLDITTTRAIMLRTHTPPVVLVTLLMLTLV
                     CSLLIGNGLARKRTCGAVLHTVGFALVLTVTLYVILDLDHPRAGLIQLDYADQALADV
                     LAGMK"
     gene            complement(835..>2211)
                     /locus_tag="PLQ18_11190"
     CDS             complement(835..>2211)
                     /locus_tag="PLQ18_11190"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF039218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HNE02258.1"
                     /translation="LDPLPAYYLLPTRVWQFALGALVYLHFGCQTPPAVAPGLLRPAS
                     WLGLAAILAAALLFDGQSGYPGFLAALPSVGAAVVLASGAHLSRSGVGWLLSRAPMQA
                     IGRVSYVWYLWHWPVLLLGAAVLDASRLEVRAGLALLALLLAALTHVLIEAPLRDARR
                     HPLPVRAVLAGTMLTMFITSGLALNWHEDSLQEAGNAQQQPDEQARLDAPVIYAMGCD
                     DWFHADTVKACEFGTGKAGKTAVVLGDSIGLQWFPAFESVFVPRDWRLLVLTKSSCPM
                     VDEPIFYARIGRRYEECERWRANALQAIAQLHPDMVILGSTYTYDFSESQWREGTARL
                     LTPLAAAAGRVYLLRSTPVLPFDAPSCLAPRGWLYQQIPDQDRCAAALRDPRHDDVDR
                     WIRSAAAAFSNVKVVDMSDAVCPGGVCRAQQHGMVAFRDTQHLSAGFARSLSDDLARR
                     LNPDAAGL"
BASE COUNT          357 a          755 c          767 g          332 t
ORIGIN      
        1 gagacggaac gcgagcgcgt cgtcctgaac acgatcgaga ccgcgatcta tgccttgctc
       61 gggctgatga tcgcgttcac gttttccgga gcggccgagc gcttcgatgc ccggcgaagc
      121 ctcacggtgc aggaaggcaa cgccatcggc acggcttacc tgcggctcga cctgctgccg
      181 gccgcggtgc agccggcatt gcgcgaaaag ttccggcgct acaccgaggc gcggatcgcg
      241 gtttatcagg cgctgccgga catcgaggcg tcgaacacgc acgcggctcg tgccggtgcg
      301 ctgcaaggtc agatctgggc ggaggccgta gcggccctga aggatgcccc gcccgcctca
      361 tcgctgctgc tgctgccggc gttgaacgac atgctggaca tcacgacgac ccgggccatc
      421 atgctgagaa cccacacgcc gccggtcgtc ctggtcacgc tgctgatgct gacgctggtc
      481 tgttcgctgc tgatcggcaa tgggctggcc cgcaagcgca cctgcggcgc cgtcctccat
      541 acggtcggat ttgcgctcgt gctgacggtc accctgtacg tcattctcga tctcgatcat
      601 ccgcgcgccg gcctgattca actggattat gccgatcagg cgctggccga tgtcctcgcc
      661 ggcatgaaat gacggcggcg ctggcattgt tcgccggccg tcccgcaatg gatgccgcct
      721 tcccggcccg gggcctctgc gatccgcgat cgctcatgcg gtcaccggcc gtgatcccgg
      781 tgcccgttgt cgggaatcac ggacgcagcc ggcgtgtggc agcgaactgc cggatcacag
      841 gccggcggca tcgggattca gccgccgggc cagatcgtcg ctcagggagc gcgcaaaccc
      901 ggcgctcagg tgctgcgtgt cgcggaaggc gaccatgccg tgctgctgcg cgcggcagac
      961 gccgccgggg cagacggcgt cgctcatgtc gacgaccttc acattgctga aggccgcggc
     1021 ggcggaccgg atccagcggt ccacgtcgtc atggcggggg tcccggagcg cagcggcgca
     1081 tcggtcctga tccgggatct gctggtacag ccatccgcgc ggggccaggc aggacggcgc
     1141 atcgaagggc aggaccggcg tcgagcgcag caggtagacc cggccggccg cggcggcgag
     1201 cggggtcagc agccgtgccg tgccttcccg ccactggctt tcgctgaaat cgtaggtgta
     1261 ggtcgagccg aggatcacca tgtccgggtg cagttgcgcg atggcctgca gggcgttcgc
     1321 gcgccagcgt tcgcattcct cgtagcggcg cccgatccgg gcatagaaga tcggctcgtc
     1381 caccatcggg cacgacgatt tggtcagcac cagcagacgc cagtcccggg gaacgaacac
     1441 ggattcaaac gctggaaacc attgcagacc gatgctgtcg ccgagcacga ccgcggtctt
     1501 tcccgccttg ccggtgccga attcgcaggc tttcaccgta tcggcgtgaa accagtcgtc
     1561 gcagcccatt gcatagatca ccggcgcatc gaggcgggcc tgttcatcgg gttgttgctg
     1621 cgcgtttccg gcttcctgca ggctgtcctc gtgccagttc agcgccagtc cgctggtgat
     1681 gaacatcgtc agcatcgtgc cggccagcac ggcgcgcacg ggcagcgggt ggcgccgggc
     1741 atcgcgcagt ggcgcttcga tcagcacgtg cgtcagggcg gcgagcagca gtgccagcag
     1801 cgcaagccct gcgcgcacct ccagccgcga cgcatccagc acggcggcac cgagcagcag
     1861 caccggccag tgccacagat accagacgta ggaaacccgc ccgatcgcct gcatcggcgc
     1921 ccgcgacagc agccagccga cgccggatcg cgacaggtgc gcaccgcttg ccagcaccac
     1981 cgccgcgccc acgctcggca gggcggcgag gaagccgggg tagcccgact ggccgtcgaa
     2041 cagcagtgcg gccgcgagga tggccgcgag ccccagccag ctcgccgggc gcagcaggcc
     2101 gggggcgacg gccgggggcg tctggcagcc gaagtgcaga tacaccagcg cgcccagcgc
     2161 gaactgccag acgcgggtcg ggagcaggta ataggccggc agcggatcga g
//
LOCUS       DAOQJH010000522         2207 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_38173_length_2207_cov_3.302974, whole genome shotgun sequence.
ACCESSION   DAOQJH010000522 DAOQJH010000000
VERSION     DAOQJH010000522.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2207)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2207)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2207
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38173_length_2207_cov_3.302974"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..853
                     /locus_tag="PLQ18_11195"
     CDS             <1..853
                     /locus_tag="PLQ18_11195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02259.1"
                     /translation="CATLAQLSLETHTDFRCPDPLRIRMQHWQSAQSVPAAVPLAAGM
                     PAPAANDHRRSLAFDVDLLQALIELDRMPAGQTAAIDKALQAYGLALAQAERLIPVTA
                     LMLLGAALRSLLACEDYDATLRDRLLAEIALLSGPDTGERAAGSSDPATGLLPGRLLR
                     EWLDDLFGCLGRSGQGDPLRGGNWAARSHLRLLGFLFPLLLKARLARLGLYRISGTDI
                     ALVGCFERLTAAEPHLLGARRAGLQAPLATAASIVQGALTPDPQVAAFEDALAWCINR
                     NLLMPDP"
     gene            1014..1217
                     /locus_tag="PLQ18_11200"
     CDS             1014..1217
                     /locus_tag="PLQ18_11200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02260.1"
                     /translation="MNGYFSKRDVQARMLKELRDARARGASGAEQLAILHRWAPFLAD
                     FDSDESDSDDSDGDLRDESEPVS"
     gene            1600..2052
                     /locus_tag="PLQ18_11205"
     CDS             1600..2052
                     /locus_tag="PLQ18_11205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007425152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF29 domain-containing protein"
                     /protein_id="HNE02261.1"
                     /translation="MGANLYDTDFCAWAQRQAELIRAGRLAEVDFEHVAEEIESMGRS
                     EAREAVSRLTVLLAHLLKWQHQPERREPEISGRSWRRTLTEQRRSLAKLFRDSPSLRA
                     RLPELIVDAYPDAAEFARLETGLIDPPWSADECPYSPGEVMADDFLPQ"
BASE COUNT          364 a          738 c          761 g          344 t
ORIGIN      
        1 ctgcgcgacg ctggcgcagc tgagcctcga aacacacacc gacttccgct gcccggaccc
       61 gctgcgcatc cgcatgcagc actggcaatc ggcgcagagc gtccctgccg ccgtgccgct
      121 ggcagcgggg atgccggcac cggcggcaaa cgaccaccgc cgcagtctgg ccttcgatgt
      181 tgacctgctg caggcgctga tcgaactcga ccggatgccg gccgggcaga ccgccgccat
      241 cgacaaggca ctgcaggcct acgggctggc gctggcacag gccgagcggc tgattccggt
      301 gactgcactg atgctgctcg gtgctgcgtt gcgcagcctg ctcgcctgcg aggactacga
      361 cgcgacgctg cgcgatcggc tgctggccga gatcgccttg ctgtcaggac ccgataccgg
      421 cgaacgggca gccggcagca gcgaccctgc caccggcctg ctgcccggtc gtttgctgcg
      481 ggaatggctg gatgatctgt tcggctgcct gggccgcagc ggtcaggggg atccgctgcg
      541 cggcggcaac tgggcggcgc gcagtcacct gcgcctcctc ggtttcctgt tcccgctgct
      601 gctgaaggcc cggctcgcgc ggctgggcct gtaccggatc agcggcaccg acatcgccct
      661 ggtcgggtgt ttcgagcgcc tgacggcagc cgaacctcac ctgctcggcg cccggcgcgc
      721 gggtctgcag gcaccgctgg cgaccgcggc cagcatcgtg cagggcgccc tgactcccga
      781 cccgcaggtc gcggccttcg aggatgcgct ggcctggtgc atcaaccgca acctgctgat
      841 gccggatccc tgagccgatc cgggcttgta tccgatcagc atcggatcag ggcctgatca
      901 attgaattga ttgaaaaatt agattgatcc tgattcagga taaggaaact gcatccggaa
      961 agggctatat agcactgcaa gaacagcaac aaatgtcgca atggaggcga gccatgaacg
     1021 gctacttttc caaacgcgat gtgcaagccc gcatgctcaa ggaactgcgc gatgcccgtg
     1081 cccggggagc cagtggcgcc gagcagctcg caatcctgca cagatgggca ccgtttctgg
     1141 cggacttcga cagcgacgag tctgacagcg acgattccga cggcgatctg cgggacgaaa
     1201 gcgaaccggt ttcatgagcg gcttcagcaa cgggttccgg cctcagacat gtcccgagga
     1261 ttccggcaac tgcctgggct gcagcttccg cacggggttc gccgtgcctt gcatcgactg
     1321 cgtgagtgcc aggtgcgtgc tgaaccacac gttgcccgga tcggtcgtgc atgccgtctg
     1381 caagcggcac gacggtattc cgcagatgcg tgccgcgccc gaagcccggg caatgtacgt
     1441 ccgggcgctg gtggatggca cgttggccgc tgcaacgcac ggggtgccaa gccgggcttg
     1501 aggccgggcc gcttgctgcc ggggagccgc cggcgacgct ggtagactcg ccgcagcacg
     1561 gtgcgggacg gcgcaccgca tgaccggggg acctgcagca tgggtgcgaa cctgtacgac
     1621 accgacttct gcgcctgggc gcagcgacag gctgagctga tccgcgccgg ccggctggca
     1681 gaagtggatt tcgagcacgt cgccgaggag atcgagagca tgggcaggag cgaggcacgc
     1741 gaggccgtca gccgcctgac cgtgctgctc gcgcacctgc tgaaatggca gcaccagcca
     1801 gagcggcggg agcccgagat cagcggccgg agctggcggc gcacgctgac cgagcaacgc
     1861 cgcagcctcg cgaagctgtt ccgtgacagc ccgagcctgc gtgcccggct gccggagctg
     1921 atcgtcgatg cctaccccga tgcggccgag ttcgcacgcc ttgagaccgg cctgatcgac
     1981 ccgccgtggt cagcggatga atgcccgtac agccccggcg aagtgatggc cgacgacttc
     2041 ctgccgcagt agacggcgcc acgcgcgctg aagccgtcgc ccccatccgg gtgctgcgcc
     2101 ggtgctgcgc gtgctcgccg ggcggcccgg acacggcggg gccactgcgg ccgcggatgc
     2161 gctgaaatct gacgcaggtc actgatttgt cagtgactgg tcccggc
//
LOCUS       DAOQJH010000523         2204 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_38273_length_2204_cov_4.105165, whole genome shotgun sequence.
ACCESSION   DAOQJH010000523 DAOQJH010000000
VERSION     DAOQJH010000523.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2204
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38273_length_2204_cov_4.105165"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..742
                     /locus_tag="PLQ18_11210"
     CDS             <1..742
                     /locus_tag="PLQ18_11210"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF016362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HNE02262.1"
                     /translation="HGSAPEATVVPAPPPAASDTPADTRTASPVAPGSAAVWTTGLLA
                     ALDWRRFEELCAGYFRLRGYRTEQRPPGSDGGIHIELYRPDTGDRLLGILLCRACGNG
                     GCTGIRELRELYGMMTAEAAALGLYVSRSGYSAEAVAFAQGKPLRLLDPSGLVALLRK
                     LPAEASAALYAEIVRDDYATPSCPQCGDKMLLRTLTSGPRAGTRFWGCRHYPDCRGLM
                     PLRAENVTVAVPVHAFAEPGRPPRAAAS"
     gene            787..1395
                     /locus_tag="PLQ18_11215"
     CDS             787..1395
                     /locus_tag="PLQ18_11215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005901467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF924 family protein"
                     /protein_id="HNE02263.1"
                     /translation="MLPDEAAELLDFWFGPLPDAGSLDPVRAAHWFLDGRAWDTPIRT
                     RFGALIERAARGELDDWPASPRGRLALILLLDQCPRHVYRATAAAFASDRLAQAQALT
                     GIVRGDDLALHPVERLFHYLPLEHAEDAVLQAASVAAFTRLHAETPPALQQQSAGWLD
                     YAERHRAVIERFGRFPDLNALLGRQTTAAEQDFLASPGSSFL"
     gene            complement(1842..>2204)
                     /locus_tag="PLQ18_11220"
     CDS             complement(1842..>2204)
                     /locus_tag="PLQ18_11220"
                     /inference="COORDINATES: protein motif:HMM:NF014195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HNE02264.1"
                     /translation="LFGKVYPKAEIDLSSERDFEGSGSADQSNSITGTITAVVTEVYP
                     NGNLQIRGEKKVTLNQGDEFVIVQGVVRPDDIAADNSIASGQVADAQITYAGKGAVAD
                     ANTAGWLTRFLIHAFFPI"
BASE COUNT          339 a          789 c          707 g          369 t
ORIGIN      
        1 gcacggcagc gcaccggaag ccacggtcgt gccggcccca ccaccggctg ccagcgacac
       61 cccggcagac accagaacgg caagccctgt cgcccccggc agcgcagcgg tctggacgac
      121 cggactgctc gcggcactcg actggcgtcg cttcgaggaa ctctgcgccg gttacttccg
      181 gctgcggggc tatcgcaccg aacagcggcc gcccggcagt gatggcggca tccacatcga
      241 gctgtaccgg cccgacaccg gtgatcgcct gctcggcatc ctgctctgcc gcgcctgcgg
      301 caatggcggc tgcaccggca tccgcgaact gcgggagctg tacggcatga tgacggccga
      361 agcggctgcg cttggcctgt atgtcagccg cagcggttac agcgccgagg ccgtggcctt
      421 cgcgcagggc aagcccctgc ggctgctcga cccgtccggt ctggtcgcac tgctgcggaa
      481 actgccggcc gaagccagcg ccgcgctgta cgccgaaatc gtccgggatg actacgcgac
      541 gccgagctgc ccgcagtgcg gcgacaagat gctgctgcgc acgctcacca gcgggccccg
      601 cgccggcacc cgcttctggg gctgccggca ttaccccgac tgtcgcggcc tgatgccgct
      661 gcgcgccgaa aacgtcacgg tcgccgtgcc ggtgcatgcc ttcgcggagc ccggccgtcc
      721 cccccgggct gccgcttcct gagcggcgat ccatactgac gggcccgtgt ccgacgcgag
      781 cccgccatgc tgcccgacga agccgccgaa ctgctggatt tctggttcgg ccccctgccc
      841 gatgccggca gcctggatcc cgtgcgcgca gcgcactggt ttctcgatgg ccgcgcctgg
      901 gacacgccga tccgcacgcg tttcggtgcg ctgatcgaac gcgccgcccg cggcgaactc
      961 gacgactggc cggcgtcgcc gcgcgggcgt ctggcgctga tcctgctgct cgatcaatgt
     1021 ccgcggcatg tgtaccgcgc cacggccgcg gccttcgcca gcgatcgact cgcgcaggct
     1081 caggccctga ccggcatcgt gcgcggcgat gacctcgccc tgcacccggt cgagcgcctg
     1141 ttccattacc tgccgctcga acacgccgag gacgccgtgc tgcaggccgc ctcggtcgcc
     1201 gccttcaccc ggctgcacgc tgaaaccccg ccggcgctgc agcagcagag cgccggctgg
     1261 ctcgattacg ccgaacgcca ccgggccgtg atcgaacgct ttggccgctt tccggatctc
     1321 aatgcgctgc tcggccggca gacgacggcg gccgaacagg actttcttgc cagccccgga
     1381 agttcctttc tctgaaccgg cgcgcgaaat tcccggcgcg tgcagggctc ctgcacttcg
     1441 attgacccag atcaatatcc ggggctggca cggtggcgga acaggattaa gctgttcgtc
     1501 agtcaggcaa ggaaatgtat ggaggcacta tcgaaggcca gattcgcggt gatgctgaac
     1561 aggccctcat cctacggtga atccggtaac gcccttcact gttccgaccg gcgcagaccg
     1621 ttgcagatac gagcttggat cattccggat actgtttgcc atcaaaaccc tgagcgcccc
     1681 cgcttccctg atccggaacg ggggcgcggt gtttttggag cctgaccggc gaatccggat
     1741 caccggaccc gccggaatcg ggttttccgg cccggacggg gtcgttcttc gaaaccgccc
     1801 ggcccgctcc cggaaatccg ctgcgtcagc cgcgcgtcgc gtcagatcgg gaagaacgcg
     1861 tggatcagga agcgcgtcag ccagccggcg gtattggcat cggcgaccgc gcccttgccg
     1921 gcgtaggtga tctgcgcatc ggcgacctgg ccggaggcga tcgaattgtc ggcggcaatg
     1981 tcatcgggcc ggaccacgcc ctgcacgatc acgaattcgt cgccctggtt cagcgtgacc
     2041 ttcttttcgc cgcgaatctg caggttgccg ttcggataaa cctcggtgac caccgcggtg
     2101 atcgtgccgg tgatgctgtt ggactgatcg gcactgcccg aaccctcgaa atcgcgttcg
     2161 ctgctcagat cgatctcggc tttcggatac accttgccga acag
//
LOCUS       DAOQJH010000524         2201 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_38412_length_2201_cov_3.136067, whole genome shotgun sequence.
ACCESSION   DAOQJH010000524 DAOQJH010000000
VERSION     DAOQJH010000524.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2201)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2201)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2201
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38412_length_2201_cov_3.136067"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1466
                     /locus_tag="PLQ18_11225"
     CDS             <1..1466
                     /locus_tag="PLQ18_11225"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018233248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HNE02265.1"
                     /translation="SGSVGLITYMRTDSVNLAAEALTEIRELIARRYGAHNVPPEPRV
                     YKTKAKNAQEAHEAIRPTSVAIEPEQVRAHLSAEQFKLYELIWKRTVACQMIPATLDT
                     VSVDLACGPGKGNTFRASGSTIADPGFLAVYEEGVDDARTREADDEEGGRVLPPLAVG
                     DVVELRAIRAEQHFTEPPPRYSEASLVKALEEFGIGRPSTYAAILSTLQAREYAVIDG
                     RRFVPTDVGRIVNRFLTQHFTQYVDYDFTARLEDELDEVSRGEKEWVPLMQEFWGPFR
                     ERCEDKEGSVSRTEVVQARELGSDPKTGKPVSVRIGRFGPFVQIGTKDDADKPQFASL
                     RPEQRMDSITLEDALELFKLPRDLGKTADGEPIKASVGRFGPYVQYGKKYVSLKEDDP
                     YSVTRERALELIAAHKEAEANKYIRVFDGSPVQVLNGRYGPYITDGKKNAKIPKYREP
                     AALTLRECLELLAAAPDKKPGARGAARGGARSKAKAG"
     gene            1466..2020
                     /locus_tag="PLQ18_11230"
     CDS             1466..2020
                     /locus_tag="PLQ18_11230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015280198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HNE02266.1"
                     /translation="MAQIALRRAARVLAAGGIVAYPTEAVYGLGCDPLDFDAVARLLA
                     LKRRPVEKGVILIGASLAQLEPWLLPLDAAQRARIGDTWPGPNTWLVPCRRSTPYWLR
                     GAHDTLAVRVTAHPLAAALCRAFGGAIVSTSANLAGRSPARSALAVRSRLGSAVDLVL
                     NGALGGQARPTTIRSLATGEIVRA"
BASE COUNT          335 a          822 c          761 g          283 t
ORIGIN      
        1 gctcgggcag cgtcggcctg atcacctaca tgcgcaccga ctcggtcaac ctcgccgccg
       61 aggcgctgac cgagatccgc gagctgatcg cacgcaggta cggggcgcac aacgtgccgc
      121 ccgagccgcg cgtctacaag accaaggcca agaacgccca ggaagcgcac gaggcgatcc
      181 gcccgacctc ggtcgcgatc gagcccgagc aggtgcgcgc gcacctctcc gccgagcagt
      241 tcaagctcta cgagctgatc tggaagcgca cggtcgcctg ccagatgatc ccggcgacgc
      301 tcgacacggt ctcggtcgat ctcgcctgcg ggcccggcaa gggcaacacc ttccgcgcca
      361 gcggctcgac catcgccgac ccaggcttcc tcgccgtcta cgaggaaggc gtcgacgatg
      421 cccgcacgcg cgaggccgac gacgaggagg gcgggcgcgt gctgccgccg ctcgcggtcg
      481 gcgacgtggt cgagctgcgc gcgatccgcg ccgagcagca cttcaccgag ccgccgccgc
      541 gctacagcga ggcgagcctg gtcaaggcgc tggaggagtt cggcatcggc cggccctcga
      601 cctacgccgc gatcctctcg acgctgcagg cgcgcgaata cgcggtcatc gacggccgcc
      661 gcttcgtgcc gaccgatgtc gggcgcatcg tcaaccgctt cctgacccag cacttcacgc
      721 agtacgtcga ttacgacttc acggcccggc tggaggacga actcgacgag gtctcgcgcg
      781 gcgaaaagga atgggtgccg ctgatgcagg agttctgggg gccgttccgc gagcgctgcg
      841 aggacaagga aggctcggtg tcgcgcaccg aggtcgtgca ggcgcgcgaa ctcggcagcg
      901 acccgaagac cggcaagccg gtgtcggtgc gcatcggccg cttcgggccc ttcgtgcaga
      961 tcggcaccaa ggacgacgcc gacaagccgc agttcgcgag cctgcgcccc gagcagcgca
     1021 tggattcgat cacgctcgag gacgcgctcg aactgttcaa gctgccgcgc gacctcggca
     1081 agaccgccga cggcgagccg atcaaggcca gcgtcgggcg cttcgggccg tacgtgcagt
     1141 acggcaagaa gtacgtgtcg ctgaaggagg acgatccgta ctcggtcacg cgcgagcgcg
     1201 cgctggaact gatcgccgcg cacaaggaag ccgaggccaa caagtacatc cgcgtgttcg
     1261 acggctcgcc ggtgcaggta ctgaacggcc gctacggccc gtacatcacc gacggcaaga
     1321 agaacgccaa gatccccaag taccgcgagc cggcggcgct gacgctgcgc gagtgcctcg
     1381 aactgctggc cgccgccccc gacaagaagc ccggtgcgcg cggcgcggcc cgcggcggcg
     1441 cccgcagcaa ggccaaggcc ggctgatggc gcagattgcg ctgcggcgcg ccgcgcgcgt
     1501 gctcgcggcc ggcggcatcg tcgcctaccc gaccgaggcc gtgtacgggc tcggctgcga
     1561 cccgctcgac ttcgatgcgg tcgcgcgtct gctggcgctg aagcgccggc cggtcgagaa
     1621 gggcgtgatc ctgatcggcg cgagcctcgc ccagctcgaa ccctggttgc tgccgctcga
     1681 tgccgcgcag cgcgcgcgca tcggcgacac ctggcccggc ccgaacacct ggctggtgcc
     1741 gtgccggcgc tcgacgccgt actggctgcg cggcgcccac gacacgctcg ccgtgcgtgt
     1801 caccgcgcat ccgctggccg cggcgctctg ccgggcgttc ggcggcgcga tcgtctcgac
     1861 cagcgccaac ctcgccggcc ggtcgccggc gcgcagcgcg ctcgccgtgc gcagccggct
     1921 cggcagcgcg gtcgatctgg tgctcaacgg cgccctcggc ggccaggccc ggccgacgac
     1981 gatccgctcg ctcgccaccg gcgagatcgt gcgcgcctga gcctctgggc gccgcgccgc
     2041 tgcgcgcgtt accctcgcgc tcccgtcctg ccgccggagc gcccccgatg ccgaccgccc
     2101 tgcccgtgtc cgcgatccgc gattacctgc tgacgctgca ggatcgcatc tgcgccgcca
     2161 tcgccgccga ggatggcgcg gccggcttcc gccatgacga c
//
LOCUS       DAOQJH010000525         2194 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_38624_length_2194_cov_4.710612, whole genome shotgun sequence.
ACCESSION   DAOQJH010000525 DAOQJH010000000
VERSION     DAOQJH010000525.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2194)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2194)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2194
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38624_length_2194_cov_4.710612"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="PLQ18_11235"
     CDS             <1..201
                     /locus_tag="PLQ18_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017354422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNE02267.1"
                     /translation="FLRFCLACTPLAHDAQRRLDDGILRALWLSPDELCAYRLRSPLV
                     RPCLDDWLAGERLPLTLLRTIG"
     gene            complement(339..1166)
                     /locus_tag="PLQ18_11240"
     CDS             complement(339..1166)
                     /locus_tag="PLQ18_11240"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNE02268.1"
                     /translation="MKTTNHGSTAIAWQDAGHGEPALLCLPGWCCEQTVFADFVRLAA
                     PHRRTLTLDWRGHGASAAAEAEFGLDGLLEDALAVIEASGVTQVVPVASSHAGWVAIE
                     LRRRLGERVPALVLIDWLVMEPPSAFFGALNGLRDAQAWQDTRDLLLAMWLPEGVSPV
                     LAQQIRAQTGRHDASMWARAAHSIAAAYRAFGTPMRALSELPDPPPVLHLASRGAPVE
                     ALREQQVFASVHPWFTVHRLAARSHFPMLEAPAATAAEVEGFLQRVQGWESVDLGCA"
     gene            complement(1342..2016)
                     /locus_tag="PLQ18_11245"
     CDS             complement(1342..2016)
                     /locus_tag="PLQ18_11245"
                     /inference="COORDINATES: protein motif:HMM:NF012273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HNE02269.1"
                     /translation="MSLPELVSFPLCPFVQRAVIVLREKQVPFTIRYIDLDNPPDWFL
                     ALSPLGKVPLLQVGDTVLFESAVIAEYLDETHPPALMPADPLARAQARAWIEFASNLL
                     GRQYRMLVAKDQAGFDEEFAAAHRELAFVERAHADGPMFLGGDTLSLVDAGFAPFFMR
                     LDLMHRARPLTLLDDYPKLRRWAAALADRDSVRRSVPEDFNDGFRRRFAGGWFGTAFA
                     DTAAMH"
BASE COUNT          340 a          779 c          769 g          306 t
ORIGIN      
        1 ttcctgcgtt tctgcctcgc ctgcacgccg cttgcgcacg atgcgcagcg ccggctcgat
       61 gacggcatcc tgcgcgcgct gtggctcagc cccgatgaac tctgcgctta ccggctgcgc
      121 agcccgctgg tgcgcccctg cctcgatgac tggctggccg gcgagcgcct gccgctgacg
      181 ctgctgcgca cgatcggctg agcgggcccc cggaaggctg cgctgcgcac cggcagccgg
      241 gccaaccggc gacgctggcc gccccgtcag cgcaaagcgt tcgcggcctg ccgtcccgaa
      301 gccgctgcgg cggctccgtg gggcacagac tgccggcttc acgcgcagcc gagatcgacc
      361 gattcccagc cctgcacgcg ctgcaggaag ccttcgacct cggccgccgt cgcggccggg
      421 gcttcgagca tcgggaagtg cgatcgggcc gcgaggcgat gcacggtgaa ccacggatgc
      481 accgacgcga acacctgctg ctcgcgcagc gcctcgaccg gcgcgccccg cgaggccaga
      541 tgcagcaccg gcggcgggtc cggcagctct gacagcgccc gcatcggcgt gccgaaggcc
      601 cggtaagcgg cagcgatcga atgggccgcg cgcgcccaca tcgacgcatc gtggcgcccc
      661 gtctgcgcgc ggatctgctg cgcgagcacc ggcgacaccc cttcgggcag ccacatcgcc
      721 agcagcaggt cgcgcgtgtc ctgccaggcc tgcgcatcgc gcaggccgtt cagcgcgccg
      781 aagaacgccg agggcggctc catgaccagc cagtcgatca gcaccagcgc cggcacgcgc
      841 tcgccgagcc gccggcgcag ctcgatcgcc acccagccgg cgtgcgaact cgccaccggc
      901 acgacctgcg tcaccccgct cgcctcgatc accgccagcg catcctccag caggccgtcg
      961 aggccgaact ccgcctcggc cgcggccgag gcgccgtggc cgcgccagtc cagcgtcagc
     1021 gtgcgccggt gcggggcggc aagccggacg aagtcggcaa acaccgtctg ctcgcagcac
     1081 cagcccggca ggcacagcag ggcaggctcg ccatggccgg cgtcctgcca ggcgatcgcg
     1141 gtactgccat gattcgtggt tttcattgga tcacggtcct ctatctcctg gcggtactcg
     1201 ccggtcacag caacggccgg cgggagcgag ccggccggcc gttggctttg gcaccggtga
     1261 ggacgcgatg ggcgtgggtc cggtgcgctg tttcaggaac tgagcctatt ctggtgcagc
     1321 gagttcccgt ttcgcatcaa tctagtgcat ggctgccgta tctgcgaatg ccgtaccgaa
     1381 ccagcctccg gcaaagcgcc ggcggaagcc gtcgttgaag tcctccggca ccgagcggcg
     1441 caccgaatcc cgatcggcga gcgccgccgc ccagcgcctg agcttcgggt aatcgtcgag
     1501 cagcgtcagt ggccgggcgc gatgcatcag atcgaggcgc atgaagaacg gcgcgaagcc
     1561 ggcatcgacc agcgacagcg tgtcgccgcc gaggaacatc ggtccgtcgg cgtgcgcacg
     1621 ctcgacgaag gcgagttcgc ggtgtgcggc ggcgaattcc tcgtcgaagc cggcctgatc
     1681 cttcgcgacc agcatccggt actggcggcc gagcaggttc gatgcgaact cgatccaggc
     1741 gcgggcctgc gcgcgcgcca gcggatcggc cggcatcagc gccggcggat gcgtctcgtc
     1801 gaggtattcg gcgatcaccg ccgactcgaa cagcacggtg tcgccgacct gcagcagcgg
     1861 caccttgccg agcggcgaca gcgccaggaa ccagtccggc gggttgtcga ggtcgatgta
     1921 gcggatcgtg aacggcacct gtttttcgcg cagcacgatg acggcacgct ggacgaaggg
     1981 gcagagcgga aagctgacga gttcgggcag gctcatgaac ggactccggt tcgggattcg
     2041 gggggaggat cgggtccggc ggacagcgcg cgggcaggcc atgggaccac agtcgcagcg
     2101 cgaccgagcc atccgtgcgc gcatggccgc aagtcagcct gtcgtggtca agttttagtg
     2161 gacaccccga ggggggattt cgctgctcag gcag
//
LOCUS       DAOQJH010000526         2189 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_38772_length_2189_cov_3.440019, whole genome shotgun sequence.
ACCESSION   DAOQJH010000526 DAOQJH010000000
VERSION     DAOQJH010000526.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38772_length_2189_cov_3.440019"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..683
                     /locus_tag="PLQ18_11250"
     CDS             <1..683
                     /locus_tag="PLQ18_11250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020291227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="substrate binding domain-containing protein"
                     /protein_id="HNE02270.1"
                     /translation="ILDAVAETEDMIGSGSRQPRGTLRLAAPMSFGTLHLSPLLPRFL
                     ARYPDLRLEIDLNDRTVDLIGEGYDMAIRIGALPDSALVARRLAGMRLVTCCSPGYLH
                     GRGAPPEPEALSAHDCLPYGHTRATEWLYRRAGERIAVPVNGRLRVNNGELARDAALA
                     GLGLTQLPTFIVGAALARGELVTVLDDFAPNPGGIFAVYPQHRQAFLAIRALADFLKD
                     AFGEETWA"
     gene            762..1586
                     /locus_tag="PLQ18_11255"
     CDS             762..1586
                     /locus_tag="PLQ18_11255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02271.1"
                     /translation="MTAPVLATIAESLFADLRTVIDTGDFDSPFTRSRLWREAEKLCN
                     VDASGGLLAKAWLAAMDRDVDTLCRHTQTMIRQRPDAQTIGAIACALISAGLLPQAAD
                     LLEDRIQLLHGNGDALAVTVDVLISIGRFSVALSIAQRFAHLSARLEMVAIKLRQSIE
                     HFHQHGIDEQDFACLSETVWQTFRAFGNGRFIGRLHASLICFGDESGDSDGREHAIYV
                     RYAVASPADADLSALIDLCMAVDEALAREHPDNPAALYWTCNFVPFEPQAVGSRAA"
     gene            1583..1957
                     /locus_tag="PLQ18_11260"
     CDS             1583..1957
                     /locus_tag="PLQ18_11260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02272.1"
                     /translation="MIEPADLLELAGRLLQGTHEADWRSASSRAYYAALHAARHYCER
                     AKLSLPIGASHERVIEALKSSKSLVERQIGQLLGRLRSERNLADYQLELDYSQRQAQK
                     CHSECQRAVTKLAELAASLPPG"
BASE COUNT          371 a          736 c          702 g          380 t
ORIGIN      
        1 ccatcctcga tgcggtcgcc gagaccgagg acatgatcgg cagcggcagc cgccagccgc
       61 gcggcacgct caggctggcc gcaccgatga gcttcggcac gctgcacctg tcgccgctgc
      121 tgccgcgctt cctcgcccgc tacccggacc tgcggctgga gatcgacctc aacgaccgca
      181 cggtcgacct gatcggcgag ggctacgaca tggccatccg catcggcgcc ctgcccgact
      241 cggcgctggt cgcacgccgc ctcgccggca tgcgcctcgt cacctgctgc agccccggtt
      301 acctgcacgg ccgcggcgcc ccgcccgaac ccgaagccct gtccgcgcac gactgcctgc
      361 cctacggcca cacgcgcgcg accgaatggc tgtaccggcg cgccggcgag cgcatcgcgg
      421 tgccggtgaa cgggcgcctg cgcgtcaaca acggcgaact ggcccgcgac gccgccctcg
      481 ccggcctcgg cctgacgcag ctgccgacct tcatcgtcgg cgcagccctc gcccgcggcg
      541 agctggtgac ggtgctcgac gacttcgccc cgaaccccgg cggcatcttc gccgtgtacc
      601 cgcagcaccg gcaggccttc ctggcgatcc gggcgctggc ggattttctg aaggatgcgt
      661 tcggggagga gacgtgggcg tgaggcggca gtcggccgcc tgcggcttgt ccgtccggac
      721 ggccggcacc agaatgcaga aggtttgcgg aggatgtacc gatgactgca cctgtactgg
      781 ccacgattgc ggagtcttta tttgctgatc tgcgaacggt cattgatacg ggcgatttcg
      841 acagtccgtt cacccgttca agactctggc gggaggctga aaaactctgc aatgtcgatg
      901 catcgggtgg actgcttgcc aaagcctggc ttgcggcgat ggaccgtgat gtcgatacgc
      961 tctgccggca cacgcagacg atgattcgtc agcgaccgga cgcgcaaacc atcggcgcca
     1021 ttgcttgtgc cctcatttca gccggactgc tgccgcaagc tgccgatctg ctggaagacc
     1081 gcatccagtt gttgcacggc aatggcgatg ccctggcggt tacggttgat gttctgatca
     1141 gtatcggacg attcagtgtc gcgctgagca ttgcgcagcg cttcgcacat ctgtctgccc
     1201 gtctggaaat ggtcgcgatc aagctgcggc aatccatcga gcatttccat caacacggta
     1261 tcgacgagca ggatttcgcc tgcctgagcg aaacggtctg gcagaccttt cgcgccttcg
     1321 gcaacggcag gttcatcggc cgcctgcatg ccagcctgat ctgtttcggt gacgagtccg
     1381 gcgacagtga tggccgggag cacgcaatct acgtgcgcta tgccgtggcc agtcctgcag
     1441 atgccgatct gtcagcgctg atcgatctgt gcatggccgt ggatgaggcc ttggctcgcg
     1501 agcacccgga caatcccgcc gccctgtact ggacctgcaa cttcgtgccg ttcgagccgc
     1561 aagccgtcgg aagccgggcg gcatgatcga accggcggac ctgctggaac tggccgggcg
     1621 gctgttgcag ggcacacacg aagcggactg gcgcagcgcc agcagccgcg cctattacgc
     1681 cgccctgcat gctgcccggc attactgcga gcgcgccaaa ctgtcgttac cgatcggcgc
     1741 aagccatgag cgtgtcatcg aagcactgaa gtcatcaaaa tcactggtag agcggcaaat
     1801 cggccagctg ctcggccgcc tgcgcagtga gcgcaatctg gcggattatc agcttgaact
     1861 ggattacagc cagcgtcagg ctcaaaagtg ccacagcgaa tgtcagcgcg ccgtcacaaa
     1921 actggctgaa ctggccgcat cacttccgcc cggctgatgg ctggcggggt gcggcgcatg
     1981 caggcgcatc ggcattggcg acgccggatc atgcccggca cgttttcgga tgcagattcc
     2041 gaagcccgca gcctgggttg acgcaggaaa cccggcgacc gcggcgatgg cgatccccgc
     2101 tgctgccaag gcctgcgaag ccgggcttcg ttcctcaacc cagcctgcga gctacgagac
     2161 ccggcgtttg tggcggcagg ggcgaccgg
//
LOCUS       DAOQJH010000527         2188 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_38800_length_2188_cov_3.847632, whole genome shotgun sequence.
ACCESSION   DAOQJH010000527 DAOQJH010000000
VERSION     DAOQJH010000527.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2188)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2188)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38800_length_2188_cov_3.847632"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..934
                     /locus_tag="PLQ18_11265"
     CDS             <1..934
                     /locus_tag="PLQ18_11265"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HNE02273.1"
                     /translation="TSGSQQGLDLVAKLFIDPGTEVAVEAPSYLAALQAFRFFGAEFA
                     ELPLTAHGIDPQQLRETLRRRRPAFVYLIPTFQNPSGVCYDAATRTAVAEVLRETGVA
                     LLEDEPYRELVYEPCERTPICAKLGDAAPWMVMGSFSKTGIPGLRIGYIAASDDIHPQ
                     LVRIKQCTDLHTNRIGQWWANEFLQSADYLGHLDRLRAYYRAQRDAMQAGLETHFADL
                     ARWNRPHGGLFFWVELHEALDERALLERALARKVAFMPGSAFYANPPARQGRMRLNFS
                     HSTPEQIDRGLRVLAETVRETSLPAGRRRIADQV"
     gene            complement(953..1603)
                     /locus_tag="PLQ18_11270"
     CDS             complement(953..1603)
                     /locus_tag="PLQ18_11270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019237934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HNE02274.1"
                     /translation="MTVLLRFLLSACAALSLSVLAPLPARAIPGSPVILVLGDSLSAA
                     YGMPIERGWVALLAARYAAAAPTPPRIVNASISGETTRGGLERLPALLAAHRPDLVVL
                     ELGANDGLRGLPVADLEANLARMIELSQQAGARVLLLGMRIPPNYGPRYTERFHASFA
                     GLATRYDVPLVPFFLDGVAGDPALVQDDGLHPRAEAQPRMLENIWPALDTLLHRPR"
     gene            1605..>2188
                     /locus_tag="PLQ18_11275"
     CDS             1605..>2188
                     /locus_tag="PLQ18_11275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014161817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNE02275.1"
                     /translation="MRATPGELDAATGRAVPIVRAERLGKQVASPEGRLTILEDIDLE
                     LAPGEQLAIVGASGSGKSTLLGLLAGLDLPTQGSVWLDGTELSTLDEDGRARLRAGRV
                     GFVFQSFQLLPGLTAIENVMLPLELAGRADAAALAQETLARVGLAARARHYPAQLSGG
                     EQQRVALARALAAQPAILFADEPTGNLDRRTGETV"
BASE COUNT          313 a          792 c          785 g          298 t
ORIGIN      
        1 caccagcggt tcgcagcagg gcctcgatct ggtcgcgaag ctgttcatcg atccgggcac
       61 cgaggtcgcc gtcgaggcgc cgagctatct ggcggcgctg caggccttcc gcttcttcgg
      121 cgccgagttc gccgagctgc cgctgaccgc gcacggcatc gacccgcagc agctgcgcga
      181 gacgctgcgc cggcgccggc cggccttcgt gtacctgatc ccgaccttcc agaatccgtc
      241 cggcgtctgc tacgacgccg ccacgcgcac ggcggtcgcc gaggtgctgc gcgagaccgg
      301 cgtcgcgctg ctcgaagacg agccctaccg cgaactggtc tacgagccct gcgagcgcac
      361 gccgatctgc gcgaagctcg gcgatgcggc gccgtggatg gtcatgggct cgttctcgaa
      421 gaccggcatc ccgggcctgc gcatcggcta catcgccgct tccgacgaca tccacccgca
      481 gctcgtgcgc atcaagcagt gcaccgacct gcacaccaac cgcatcggcc agtggtgggc
      541 gaacgaattc ctgcagtcgg ccgattatct cggccacctc gaccgcctgc gtgcctacta
      601 ccgcgcgcag cgtgacgcga tgcaggccgg gctcgaaacc catttcgccg atctcgcgcg
      661 ctggaaccgg ccgcacggcg ggctgttctt ctgggtcgag ctgcacgagg cgctcgatga
      721 gcgtgcgctg cttgaacggg cgctggcgcg caaggtcgcg ttcatgcccg gcagcgcgtt
      781 ctacgccaac ccgccggccc gccaggggcg catgcgcctg aacttctcgc actcgacgcc
      841 ggagcagatc gatcgcggcc tgcgcgtgct cgccgagacc gtgcgcgaga ccagcctgcc
      901 ggccgggcgc cggcgcatcg ccgatcaggt ctgagcagcg ggcgcgccgg cttcagcgcg
      961 gccggtgcag cagcgtgtcg agcgccggcc agatgttttc cagcatgcgc ggctgcgcct
     1021 cggcgcgcgg atgcaggccg tcgtcctgca ccagcgccgg gtcgccggcg acgccgtcga
     1081 gaaagaacgg caccagcggc acgtcatagc gtgtggcgag tccggcaaag ctcgcgtgga
     1141 accgttcggt gtagcgcggg ccatagttgg gcgggatgcg catgccgagc agcagcacgc
     1201 gggcgccggc ctgctgcgac agctcgatca tccgcgcgag attcgcctcc agatcggcga
     1261 ccggcagccc gcgcaggccg tcgttggcgc cgagttccag cacgacgaga tccggccggt
     1321 gggcggcgag cagcgccggc agccgttcca gtccgccgcg cgtggtttca ccgctgatgc
     1381 tggcgttgac gatccgcggc ggggtcggcg ccgccgctgc atagcgcgcc gccagcagcg
     1441 cgacccagcc gcgctcgatc ggcatgccgt aggccgcgct caggctgtcg ccgagcacga
     1501 ggatcaccgg gctgcccggg atggcacggg ccggcagcgg ggcgagcacc gacaggctca
     1561 gcgcagcaca ggcagacaac aggaaacgca ggagtacggt catcgtgcga gcaacacccg
     1621 gagaactgga tgcggcgacc ggccgcgccg tgccgattgt ccgggccgag cgcctcggca
     1681 agcaagtcgc gagcccggag ggccggctga cgatcctgga ggacatcgac ctcgaactcg
     1741 cgcccggcga gcagctggcg atcgtcgggg cttcgggctc gggcaagtcg acgctgctcg
     1801 gcctgctcgc cggcctcgac ctgccgactc agggttcggt gtggctggac ggcaccgagc
     1861 tgagcacgct cgatgaggac ggccgcgcgc ggctgcgcgc cggacgggtc ggcttcgtgt
     1921 tccagtcctt ccagctgctg ccggggctga ccgcgatcga gaacgtcatg ctgccgctgg
     1981 aactggccgg gcgcgccgat gccgcggcgc tcgcgcagga aaccctcgcg cgcgtcgggc
     2041 tggccgcgcg cgcgcggcat tacccggcgc agctctccgg cggcgagcag cagcgcgtgg
     2101 cgctggcgcg cgcgctcgcc gcgcagccgg cgatcctgtt cgccgacgag ccgaccggca
     2161 acctcgaccg gcgcaccggc gagaccgt
//
LOCUS       DAOQJH010000528         2186 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_38876_length_2186_cov_4.044111, whole genome shotgun sequence.
ACCESSION   DAOQJH010000528 DAOQJH010000000
VERSION     DAOQJH010000528.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2186
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38876_length_2186_cov_4.044111"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(210..656)
                     /gene="rplI"
                     /locus_tag="PLQ18_11280"
     CDS             complement(210..656)
                     /gene="rplI"
                     /locus_tag="PLQ18_11280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003095627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HNE02276.1"
                     /translation="MEVILLSKVENLGNLGDRVTVKSGYGRNFLIPRGKATEATPTNV
                     ARFEARRAELEAAAADMLAKAQARRDQLADLLVTMAAKAGAEGRLFGSIGPADIADVV
                     THAGYELKKSEVRMPEGPIRHVGEVEVDVHLHTDVNFKLHVNIIAE"
     gene            complement(733..1638)
                     /locus_tag="PLQ18_11285"
     CDS             complement(733..1638)
                     /locus_tag="PLQ18_11285"
                     /inference="COORDINATES: protein motif:HMM:NF021506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HNE02277.1"
                     /translation="MTGIASRVMAGRLPAVLIVVATMFVSLLLPPVLLLSGAALALVT
                     LRRGPYEGALILGAASVAAFVLGWLLFGHPGPLAGVLPGFWLPVWGLAAVLRATVSLA
                     AAFAVAAGLGWLVVTGFYALLDDPAGWWQGWLAELLKPAFEQAGAGSDAVQGLDRLIA
                     WGAPLMPGMLAANVVFSALVALLIGRWWQARLYNPGGFRSEFHGLRLGHGLALLTIVT
                     GVAAFASGALWLQCLALPLAVVWLLQGLAVLHGLALLRGWSPWALVLVYVLLLLLLPQ
                     AVALLCLLGLIDTWMNLRARAAPPG"
     gene            complement(1709..1933)
                     /gene="rpsR"
                     /locus_tag="PLQ18_11290"
     CDS             complement(1709..1933)
                     /gene="rpsR"
                     /locus_tag="PLQ18_11290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020584246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HNE02278.1"
                     /translation="MSRFFRRRKYCRFTAEGVTEIDYKDLGTLKNYITETGKIVPSRI
                     TGTKARYQRQLVTAIKRARFLALLPYTDRH"
     gene            complement(1970..>2186)
                     /locus_tag="PLQ18_11295"
     CDS             complement(1970..>2186)
                     /locus_tag="PLQ18_11295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009151404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HNE02279.1"
                     /translation="YVLMNVECSQAVLDEIESAFRFNDAVLRNLIISRPGPDTEASPL
                     AKSREGREERESRHDDVAAEVDASDDE"
BASE COUNT          372 a          769 c          761 g          284 t
ORIGIN      
        1 acactcggcg cgacctcgca agtcaggcag gcagcggatc ggcccggcgc aaccgggcgg
       61 cccgcgaatc tagcacaggc ctgtaccggc ggtgccgcag acgacgaacg gccccggcca
      121 ccggcgtcac aggacgtcgg cggccggggc cgcgcgcgga gggcctggac ttcggcgacg
      181 actgcgtcgg agccggtcct gcccggtgat tactcggcga tgatgttgac gtgcagcttg
      241 aagttcacgt cggtgtgcag gtgcacgtcg acctcgacct cgccgacgtg gcggatcggg
      301 ccttccggca tgcgcacttc ggacttcttc agctcgtagc cggcgtgggt gacgacatcg
      361 gcgatgtcgg ccgggccgat cgagccgaac agccggcctt cggcaccggc cttggcggcc
      421 atggtcacca gcaggtcggc gagctggtcg cggcgggcct gggccttggc gagcatgtcg
      481 gccgcagcgg cctccagctc ggcccggcgg gcctcgaagc gggcgacgtt ggtcggcgtg
      541 gcctcggtgg ccttgccgcg cgggatcagg aaattgcggc cgtaaccgga cttgacggtg
      601 acgcggtcac cgaggttgcc gaggttttcg accttcgaga gcaggatgac ttccatcgtc
      661 ggaactccga atgcgtggac agtgcgtgaa tgtacggttg ggcggatcag gcgattcgtt
      721 cggtacgggc attcatcccg ggggcgcggc acgggcgcgc aggttcatcc aggtatcgat
      781 cagtccgagc aggcagagca gcgcgacggc ctgcggcagc aacagcagca gcagcacgta
      841 cacgagcacc agcgcccacg gcgaccagcc gcgcagcagc gccagcccgt gcagaaccgc
      901 aagcccctgc agcagccaga ccaccgccag cggcagggcc aggcactgca gccagagcgc
      961 gccactcgcg aaggccgcca cgccggtgac gatcgtcagc aacgccagcc cgtgcccgag
     1021 acgcaggccg tgaaactcgc tgcggaaacc gcccgggttg tacagccgcg cctgccacca
     1081 gcgcccgatc agcagcgcca ccagcgccga gaacacgacg ttggctgcca gcatccccgg
     1141 catcagcggc gcgccccagg cgatcagccg gtcgagaccc tgcaccgcgt cgctgccggc
     1201 gccggcctgc tcgaaggccg gcttcagcaa ctcggcgagc cagccctgcc accagccggc
     1261 cggatcgtcg agcagcgcat aaaagccggt gaccaccagc cagccgaggc cggccgcgac
     1321 cgcgaacgcc gccgcgagcg acaccgtcgc ccgcagcacc gccgccagtc cccagaccgg
     1381 cagccagaag cccggcagca cgccggcgag cggccccgga tggccgaaca gcagccagcc
     1441 gagcacgaag gccgcgacgc tggccgcgcc gaggatcagc gcaccctcgt acggcccgcg
     1501 cctgagcgtc accagcgcca gcgccgcgcc cgagagcagc agcaccggcg gcagcagcag
     1561 cgatacgaac atcgtcgcga cgacgatcag cacggccggc agccgtcctg ccatcacccg
     1621 cgacgcgata cctgtcatga atgaacccgc ccgcaggccg cgaccgcccg gaaccggatc
     1681 aacccggtac cggcgttcgc agcgccgctc agtgacggtc ggtgtagggc agcagcgcga
     1741 ggaaacgcgc gcgcttgatc gccgtgacga gctggcgctg gtagcgcgcc ttcgtgccgg
     1801 tgatgcggct cggcacgatc ttgcccgtct cggtgatgta gttcttcagc gtgccgagat
     1861 ccttgtaatc gatctcggtg acgccttcgg cggtgaagcg gcagtacttg cggcgacgga
     1921 agaagcggga catggcggat tcgatcctgg tgcgtggaag gcggacggct tattcgtcgt
     1981 cggaggcgtc gacttcggcg gcgacgtcgt cgtgacgcga ctcgcgctcc tcgcggccct
     2041 cgcgggactt ggcgagcggc gaagcctcgg tgtccggacc cggacggctg atgatcaggt
     2101 tgcgcagcac cgcatcattg aagcggaacg cgctctcgat ctcgtcgagc acggcctggc
     2161 tgcactcgac gttcatcagc acatag
//
LOCUS       DAOQJH010000529         2184 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_38946_length_2184_cov_4.933302, whole genome shotgun sequence.
ACCESSION   DAOQJH010000529 DAOQJH010000000
VERSION     DAOQJH010000529.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2184)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2184)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2184
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38946_length_2184_cov_4.933302"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..510)
                     /gene="hflX"
                     /locus_tag="PLQ18_11300"
     CDS             complement(<1..510)
                     /gene="hflX"
                     /locus_tag="PLQ18_11300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005961351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HNE02280.1"
                     /translation="MFERPRAGERAVLVHLALNAQIDPREQAEFEELAKSAGADIVGQ
                     VSGTRRAPDARYFVGSGKAEEIRDRVAASGAELAIFDHELSPSQERNLEALLQCRVID
                     RTGLILDIFATRARSFEGKLQVELAQLEHLATRLVRGWTHLERQRGGIGLRGPGETQL
                     ETDRRRIYRR"
     gene            complement(552..806)
                     /gene="hfq"
                     /locus_tag="PLQ18_11305"
     CDS             complement(552..806)
                     /gene="hfq"
                     /locus_tag="PLQ18_11305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005223727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HNE02281.1"
                     /translation="MAKGHSLQEPFLNALRKERIPVSIYLVNGIKLQGQIESFDQFVV
                     LLKNSVSQMIYKHAISTVVPARNVRLHLAEDAGADGHDDA"
     gene            complement(886..1836)
                     /gene="miaA"
                     /locus_tag="PLQ18_11310"
     CDS             complement(886..1836)
                     /gene="miaA"
                     /locus_tag="PLQ18_11310"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012970436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HNE02282.1"
                     /translation="MTADPRPLAVCLMGPTASGKTDLAIELLERAPGGFEIVSVDSAM
                     IYRGMDIGTAKPDAGTLARAPHRLIDLVDPAESYSAGRFRRDALAAMDEIRAAGRVPL
                     LVGGTMFYFRALAQGLAELPEADAGVRAALDAELAAHGAAALHAQLAAIDPVAAARIH
                     PNDPQRVQRALEVWRLSGRTLSDWHACQHLAGCPYRLLNFVLLPADRGLLHARIEQRF
                     DRMLACGFIEEVARLRARADLHPGLPAMRAVGYRQVWEHLDGLTDRTELRQRGIQATR
                     QYARRQLTWLRGETGAVPLAPATAVNTVLRTLGALRDHPG"
     gene            complement(1839..>2184)
                     /gene="mutL"
                     /locus_tag="PLQ18_11315"
     CDS             complement(1839..>2184)
                     /gene="mutL"
                     /locus_tag="PLQ18_11315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007149053.1"
                     /note="This protein is involved in the repair of
                     mismatches in DNA. It is required for dam-dependent
                     methyl-directed DNA mismatch repair. Promotes the
                     formation of a stable complex between two or more
                     DNA-binding proteins in an ATP-dependent manner without
                     itself being part of a final effector complex; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA mismatch repair protein MutL"
                     /protein_id="HNE02283.1"
                     /translation="LGLGIVLAGPDRALLRELPALLADGDIEALVRGVLADLAEHGSS
                     SRVGERHNELLATMACHGSVRARRRLTLAEMNALLRAMEATERSDQCNHGRPTWVQLG
                     LGELDRLFLRGR"
BASE COUNT          318 a          754 c          781 g          331 t
ORIGIN      
        1 gcggcggtag atgcggcggc ggtcggtttc cagctgggtt tcgccgggac cgcgcaggcc
       61 gatgccgccg cgctggcgtt cgaggtgggt ccagccgcgc accaggcgcg tggccaggtg
      121 ttcgagctgc gcgagctcga cctgcagctt gccctcgaag ctgcgcgcac gggtcgcgaa
      181 gatgtcgagg atcagcccgg tgcggtcgat gacccggcac tgcagcagcg cttcgaggtt
      241 gcgctcctgc gacggcgaca gttcgtgatc gaagatcgcg agttcggcgc cgcttgccgc
      301 gacccggtcg cggatttcct cggccttgcc gctgccgacg aaatagcgcg cgtccggcgc
      361 acggcgtgtg ccgctcacct ggccgacgat gtcggcgcct gccgacttcg ccagctcctc
      421 gaactccgcc tgctcccggg gatcgatctg cgcgttcagc gcgaggtgca ccagcaccgc
      481 gcgctcaccc gcgcggggtc gttcgaacaa aggcagggtc ctcggtcacg caggggtgcg
      541 gggcgacggg ttcaggcgtc gtcgtggcca tcggcgccgg cgtcctcggc gaggtgcagg
      601 cgcacgttgc gcgccggcac gacggtcgag atcgcgtgct tgtagatcat ctggctgacg
      661 gaattcttca gcagcacgac gaactggtcg aaggattcga tctggccctg cagcttgatg
      721 ccgttgacca gatagatcga gacggggatg cgctccttgc gcaaggcgtt caagaacggt
      781 tcctgaagcg aatggccctt agccatgtga tggctccttg ttgttatcga agtctggctg
      841 tctgttgccc gggcaggacg caggctgacc ggtgattctt cctgctcaac ccgggtggtc
      901 ccgcaacgca cccaaagtgc ggagcaccgt gttcacggcc gtggccggcg ccaggggaac
      961 ggcgccggtc tctccgcgca accacgtcag ctgccttcgg gcgtactggc gggttgcctg
     1021 gattccacgc tgccgcaact cggtacggtc cgtcaagcca tccagatgtt cccagacctg
     1081 acgatagccg accgcacgca tggccggcag gccggggtgc aggtcggcgc gcgcccgcag
     1141 ccgcgccacc tcctcgatga aaccgcaggc cagcatgcga tcgaagcgct gttcgatgcg
     1201 cgcatgcagc aggccccgat ccgcgggcag cagcacgaag ttcaggagcc ggtacgggca
     1261 gccggcgagg tgctggcagg cgtgccagtc gctgagcgtg cgtccgctca gccgccagac
     1321 ctcgagcgca cgctgcacgc gctgcggatc gttcgggtgg atgcgcgccg ccgccaccgg
     1381 atcgatcgcc gcgagctgcg cgtgcagcgc cgcggcgccg tgcgctgcca gctcggcatc
     1441 gagcgcggca cgcacgccgg cgtcggcctc gggcagttcg gcgagcccct gcgccagcgc
     1501 ccggaaatag aacatcgtgc cgccgaccag cagcggcacg cgcccggcgg cgcggatctc
     1561 gtccatcgcc gccagcgcat cgcgccggaa gcggccggcc gagtagcttt cggccggatc
     1621 gacgagatcg atcagccggt gcggcgcgcg ggcgagggtt ccggcatcgg gcttggcggt
     1681 gccgatgtcc atgccgcggt agatcatcgc cgaatcgacg ctgacgatct cgaacccgcc
     1741 cggcgcccgt tcgagcagct cgatcgcgag atcggtcttg ccggaggcgg tcggccccat
     1801 caggcagacg gcgagcggac gcgggtcggc cgtcatgctc agcgcccgcg caggaacagc
     1861 cggtcgagct cgccgagacc aagctgcacc caggtcggcc ggccgtggtt gcactggtcg
     1921 ctgcgctcgg tggcctccat cgcgcgcagc agcgcattca tctcggccag cgtgaggcgc
     1981 cgccgggcgc gcaccgaacc gtggcaggcc atggtcgcca gcagctcgtt gtggcgctcg
     2041 ccgacgcgcg aactgctgcc gtgttcggcc agatcggcga gcacgccgcg caccagcgcc
     2101 tcgatgtcgc catcggcaag cagtgctggc agctcacgca gcagcgcgcg gtccgggccg
     2161 gccagcacga tgccgaggcc gagg
//
LOCUS       DAOQJH010000530         2181 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_39053_length_2181_cov_3.736124, whole genome shotgun sequence.
ACCESSION   DAOQJH010000530 DAOQJH010000000
VERSION     DAOQJH010000530.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2181
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39053_length_2181_cov_3.736124"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(72..926)
                     /locus_tag="PLQ18_11320"
     CDS             complement(72..926)
                     /locus_tag="PLQ18_11320"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNE02284.1"
                     /translation="MPHTTDHLLLDDGQCLRVVRRGTQAGGRPVVCLHGWTSTHADWT
                     HWTQALAGAHPSFAWDARGHADVPLTGAPATLERMADDLDALIRHYALERPLLLGHSM
                     GALTIWMHALRHPGTAIGGYGLIDQTPKLVTGAGWRFGIYGDFSAQRNRWFIARQRED
                     FAEAVLELTADGLNARARERYEREPAKFQRSRDWLAGLAHAALTECWCSLAGADLRPA
                     LARLHAPVLLVYGDESNFYTAETARWVQAQIPHARLETYAGADHCPHFWQRDRFLGEL
                     RALLAACG"
     gene            complement(936..>2181)
                     /locus_tag="PLQ18_11325"
     CDS             complement(936..>2181)
                     /locus_tag="PLQ18_11325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006475316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNE02285.1"
                     /translation="SSWPGDYPNFLERKAAALEEEARHNALFDKKLAQEEAWIRQGIK
                     ARRTRNEGRVRALKALREERSERRELQGRVSFNLEEAAASGKLVVEAKDVSYAWDGAP
                     VVRDFSVRVLRGDRIGLIGPNGAGKTTLLNLLLGRLAPDSGSIRLGTKLQVAYFDQLR
                     AVLDPEASVIDNLAEGREFIEINGERRHVISYLQDFLFSPERTRTPVKSLSGGERNRL
                     LLARLFSQPANLLVMDEPTNDLDIETLELLEELLLDYQGTLLLVSHDRAFLDNVVTSS
                     LVFEGRGRIGEYVGGYSDWLRQRRPAAEEKATAKPAATIKTATPAPAAAPAATATPAP
                     AKKPAKLGYKEQRELDALPAQIEALEAEQAELGTRTAAAGFYQQPQAAQAAVLDRLAA
                     LERELEAAYARWDALEALKDGR"
BASE COUNT          288 a          729 c          806 g          358 t
ORIGIN      
        1 aggtagggac gcggctgccg cggcgaaggt tcccggaacg agcgggatta ccggaatttt
       61 attgcgccgc atcatccgca ggccgcgagc agcgcgcgca gctcgccgag gaagcggtcg
      121 cgctgccaga agtgcgggca gtgatcggca ccggcgtagg tttcgagacg cgcgtgcggg
      181 atctgcgcct gcacccagcg cgcggtttcg gccgtgtaga agttgctctc gtcgccgtag
      241 accagcagca ccggggcgtg cagccgcgca agcgccgggc gcaggtcggc gcctgcgagc
      301 gagcaccagc actcggtcag cgccgcgtgc gcgaggccgg cgagccagtc gcggctgcgc
      361 tggaacttcg ccggctcgcg ctcgtagcgt tcgcgggcgc gggcgttcag gccgtcggcg
      421 gtcagttcga gcacggcctc ggcgaagtcc tcgcgctggc gggcgatgaa ccagcggttg
      481 cgctgcgccg agaagtcccc gtagatgccg aagcgccagc cggcaccggt gacgagcttc
      541 ggcgtctggt cgatcaggcc gtagccgccg atcgcggtgc cgggatgtcg cagcgcatgc
      601 atccagatcg tcagcgcgcc catcgaatgc ccgagcagca gcggccgttc gagcgcgtag
      661 tggcggatca gcgcatcgag gtcatcggcc atgcgttcga gcgttgccgg cgcgccggtg
      721 agcggcacat cggcatggcc gcgcgcatcc cacgcgaagc tcgggtgagc gccggccagc
      781 gcctgcgtcc agtgcgtcca gtcggcgtgc gtcgaggtcc agccgtgcag gcagacgacc
      841 ggccggccgc cggcctgcgt gccgcgacgc acgacgcgca ggcactggcc gtcgtcgagg
      901 agcaggtgat cggtcgtgtg cggcatggcc ggggctcagc gtccgtcctt cagcgcttcg
      961 agcgcgtccc agcgcgcgta tgcggcttcg agttcccgtt cgagcgcggc gaggcgatcg
     1021 agcaccgcag cctgcgcggc ctgcggctgc tggtagaagc cggccgcggc cgtgcgcgtg
     1081 ccgagttcgg cctgctcggc ttcgagcgct tcgatctgcg ccggcagcgc gtcgagttcg
     1141 cgctgttcct tgtagccgag cttcgccggt ttcttcgccg gagccggtgt ggcggtggca
     1201 gccggcgcgg cggccggggc cggcgttgcg gtcttgatcg ttgcggccgg cttcgcggtg
     1261 gccttctcct cggccgccgg ccggcgctgg cgcagccagt cgctgtagcc gccgacgtat
     1321 tcgccgatcc ggccccggcc ttcgaacacc agcgagctgg tgacgacgtt gtcgaggaag
     1381 gcgcgatcgt gcgagaccag cagcagcgtg ccctgatagt cgagcagcag ctcttcgagc
     1441 agttcgagcg tctcgatgtc gaggtcgttg gtcggctcgt ccatgaccag caggttggcc
     1501 ggctggctga acaggcgtgc gagcagcaga cggttgcgct cgccgccgga gagcgacttc
     1561 accggtgtgc gcgtgcgctc ggggctgaat aagaagtcct gcagatagct gatgacgtgg
     1621 cggcgttcgc cgttgatctc gatgaactcg cgaccttcgg cgaggttgtc gatgaccgaa
     1681 gcctcgggat cgagcaccgc gcgcagctgg tcgaagtacg cgacctgcag cttggtgccg
     1741 aggcggatcg agccgctgtc cggcgcaagc cgcccgagca gcaggttcag cagcgtggtc
     1801 ttgccggcgc cgttcgggcc gatcaggccg atgcggtcgc cgcgcagcac gcgcaccgag
     1861 aaatcgcgca cgaccggcgc gccgtcccac gcgtagctca cgtccttcgc ctcgaccacc
     1921 agcttgccgg aggccgcggc ctcttcgagg ttgaagctga ccctgccctg cagttcgcgc
     1981 cgctcggagc gttcctcgcg cagcgccttc agcgcgcgca cgcggccttc gttgcgcgtg
     2041 cgtcgggcct tgatgccctg gcggatccag gcttcttcct gcgcgagctt cttgtcgaac
     2101 agcgcgttgt ggcgggcttc ctcttcgagc gcggcggcct tcctttcgag gaagttcgga
     2161 tagtcgccgg gccaggagct g
//
LOCUS       DAOQJH010000531         2179 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_39126_length_2179_cov_3.854049, whole genome shotgun sequence.
ACCESSION   DAOQJH010000531 DAOQJH010000000
VERSION     DAOQJH010000531.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2179
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39126_length_2179_cov_3.854049"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(295..882)
                     /locus_tag="PLQ18_11330"
     CDS             complement(295..882)
                     /locus_tag="PLQ18_11330"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNE02286.1"
                     /translation="MSALNFSLRPARVEEAAIVAGAIAEASGGYAPLAWSALAGDGDL
                     HATGARCCAADDGPHSWRNALVLETQDGAVAGVLLGCAAAQVPPVAAVLGADGSDLCA
                     PVRMQVPDTWLLVGMTIFSPWRNYGLGHRLLVQACSQARAAGFDRVSLIACEHNPATR
                     LYRREGFTVVERRPILPHPLLAYAGDVLLMVRALP"
     gene            complement(902..1744)
                     /gene="queF"
                     /locus_tag="PLQ18_11335"
     CDS             complement(902..1744)
                     /gene="queF"
                     /locus_tag="PLQ18_11335"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020831211.1"
                     /note="Catalyzes the NADPH-dependent reduction of
                     7-cyano-7-deazaguanine (preQ0) to
                     7-aminomethyl-7-deazaguanine (preQ1) in queuosine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0043867 - 7-cyano-7-deazaguanine
                     tRNA-ribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HNE02287.1"
                     /translation="MSDLSHLPLGRFVAGSEHYDPALLCTVPRAGQRAQLGFSDNTLP
                     FVGVDLWNAYELSWLTPAGKPQVAMASFAVPAESPNLVESKSLKLYLNSFTQTRLRDR
                     TDLLRRLSADLGAAFGTLIGVHLTGPEAFAAQRSEELAGQCIDELDVSPESYGPPDPA
                     LLMLATGVPRKPVEETLVSHLLKTNCLVTGQPDWASVQIRYTGQPIDRAGLLKYLITF
                     RNHHEFHEHCVERIWRDIHERCQPESLAVYARYTRRGGLDINPFRASHSARPWPENLR
                     TARQ"
     gene            complement(1906..>2179)
                     /locus_tag="PLQ18_11340"
     CDS             complement(1906..>2179)
                     /locus_tag="PLQ18_11340"
                     /inference="COORDINATES: protein motif:HMM:NF018813.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HNE02288.1"
                     /translation="APRLSVWAFGGRAAEAWWQRLPARVQTLPGLEVRLLAAAETAAL
                     AARMARTMRFDCRLQHGRCQLTDAAGTLTLQLQPRPAGGTRPAPSA"
BASE COUNT          310 a          733 c          858 g          278 t
ORIGIN      
        1 gttgacgatc agcccgccct gcaggccgag gatgccgatg aaggtgcgcc ggcggccgta
       61 gctgagcgcg ttgctcatgg tgttcacggc gcccgcaccc ggtgaaatgc tgatcagcac
      121 acaggcggcc agccaggcca gccagacgtg caggtccatg atgtcgcccc ctcgtgaaaa
      181 cgcgccggat cggcaggccg gcaagtctgc gccgcgtcgc gcagcggcca tagcggcgca
      241 tggctcgcat gcgcggccgt caggagcggg gcagggggcg gcgggcgggc cggctcaggg
      301 cagcgcgcgc accatcagca gcacgtcgcc ggcgtaggcg agcagcgggt gcggcaggat
      361 cggccggcgc tcgaccaccg tgaagccctc gcgccggtac aggcgcgtgg ccgggttgtg
      421 ctcgcaggcg atcaggctga cgcgatcgaa gccggcggcg cgtgcctgcg agcaggcctg
      481 caccagcagc cggtggccga ggccgtagtt gcgccacggc gagaagatgg tcatgccgac
      541 cagcagccag gtgtccggca cctgcatgcg caccggcgcg cagagatcgc tgccatcggc
      601 cccgagcacc gccgcgaccg gcggcacctg cgcggcggcg cagccgagca gcacgccggc
      661 gacggcgccg tcctgggttt cgagcaccag cgcattgcgc caggaatgcg ggccgtcatc
      721 ggcggcgcag cagcgggcgc cggtggcgtg caggtcgccg tcgccggcga gggccgacca
      781 ggcgagcggc gcgtagccgc ccgaggcctc ggcgatggca ccggcgacga tcgcggcttc
      841 ctcgacgcgg gccgggcgca ggctgaagtt cagcgcggac atggcggcgg ctcccggcgg
      901 gtcattgccg cgccgtgcgc aggttctcgg gccagggccg ggcgctgtgg ctggcccgga
      961 acgggttgat gtcgagtccg ccgcggcgcg tgtagcgcgc gtagacggcg agcgattccg
     1021 gctggcagcg ttcgtggatg tcgcgccaga tgcgctcgac gcagtgctcg tggaattcgt
     1081 ggtggttgcg gaacgtgatc aggtacttca gcagcccggc ccggtcgatc ggctggccgg
     1141 tgtagcggat ctgcacgctc gcccagtccg gctggccggt gacgaggcag ttggtcttca
     1201 gcaggtgcga gaccagggtt tcctcgaccg gcttgcgcgg aacgccggtg gccagcatca
     1261 gcagtgccgg atcgggcggg ccgtaggact cggggctcac gtcgagctcg tcgatgcact
     1321 ggccggcgag ttcctcgctg cgctgcgcgg cgaaggcttc ggggccggtc aggtgtacgc
     1381 cgatcagcgt gccgaaggcc gcgccgagat cggccgacag gcgccgcagc agatcggtgc
     1441 gatcgcgcag ccgggtctgg gtgaaggagt tcaggtacag cttcagcgac ttggattcga
     1501 ccaggttcgg cgattcggcc ggcacggcga aactcgccat cgccacctgc ggcttgccgg
     1561 ccggcgtcag ccatgacagc tcgtaggcgt tccacagatc gacgccgacg aatggcagcg
     1621 tgttatcgga gaagccgagt tgcgcgcgct gcccggcgcg cggcaccgtg cacagcaggg
     1681 cgggatcgta atgttcggag ccggcgacga agcggccgag cggcaggtgt gacagatcac
     1741 tcatgcggga aatccggggg aggcggcggg gggcgcggcg cgtccgggat tccggcggag
     1801 cgccggcctc ccggcggcac cccgttgcgc aggtgatcgg gccgcagcgt aacaggctgc
     1861 ccgcaagctg tgccacgccg tcgcccccga ggaggtagcg gcgcatcagg ccgacggggc
     1921 agggcgcgtg ccgccggccg ggcggggctg cagctgcagc gtcagcgtgc cggcggcatc
     1981 ggtgagctgg cagcggccgt gctgcagccg gcagtcgaag cgcatcgtgc gcgccatccg
     2041 ggcggcgagg gcggcggttt cggctgcggc cagcagccgc acctccagcc ccggcagggt
     2101 ctgcacccgc gccggcagcc gctgccacca ggcttcggcc gcgcgcccgc caaaggccca
     2161 gaccgacagc cgcggcgcc
//
LOCUS       DAOQJH010000532         2179 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_39127_length_2179_cov_3.821563, whole genome shotgun sequence.
ACCESSION   DAOQJH010000532 DAOQJH010000000
VERSION     DAOQJH010000532.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2179
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39127_length_2179_cov_3.821563"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..767
                     /locus_tag="PLQ18_11345"
     CDS             <1..767
                     /locus_tag="PLQ18_11345"
                     /inference="COORDINATES: protein motif:HMM:NF014686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HNE02289.1"
                     /translation="LLDLCRRERVELLVPTRDAELPWFAERRARFAEIGTRVLVSAPA
                     AIERCRDKRAFADFCAAHGFATPPAVTLPAAADDFPLFAKPRIGQGSGGARRIESAAG
                     LAGLDAEHWIVQRFDGGPEYTLDVCAGFDGTVLSVVPRRRLQVLAGESITGVTVMDPA
                     LVELGAAVVRALGLIGHATVQCFHDAAGSRLIEVNPRFGGGAALGFAAGADSPRWLLR
                     LARGGSIEPQLGCQRDGLYLLRHSHDVFVAAEDVCR"
     gene            767..1477
                     /locus_tag="PLQ18_11350"
     CDS             767..1477
                     /locus_tag="PLQ18_11350"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNE02290.1"
                     /translation="MPAPPAPAVVLLDLDDTLYAERDWLLSGCRAVAGCAEALLGRSA
                     AELYDWLLQRLLTEGRERLFDALLAAHGRCDPALSALLVHVFRSHRPRLTAFADVAPA
                     LAAWRARGCGLGLITDGVSCAQHAKLDGLGLRAGFDLVLCTDDLPGAARKPAPLAFQL
                     ALAHFGCAPEQAVYIGDDPSKDFLAPRALGMRTLRIDRHLPWPLQSRRDFPDSHQAHR
                     TIESLAEAAAPDASGLAA"
     gene            1504..>2179
                     /locus_tag="PLQ18_11355"
     CDS             1504..>2179
                     /locus_tag="PLQ18_11355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008064007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HNE02291.1"
                     /translation="MSCTGFEIAGRRIGPDAPPFVIAEMSGNHNQSLQRALAIVDAAA
                     AAGAHAIKLQTYTADTMTLDLDEGEFHIDDPASLWRGESLYRLYRQAYTPWEWHAPLF
                     ARAREHGLIAFSTPFDASAVDFLDSLDVPAWKVASFENTDLPLIRRMAATGRPMIIST
                     GMATVGELDETVRTARAAGCRDLVLLKCTSTYPATPADTHLLTLPHLRALFGCEVGLS
                     DHTMGLG"
BASE COUNT          275 a          811 c          761 g          332 t
ORIGIN      
        1 cgctgctcga tctgtgccgg cgcgagcgcg tcgagctgct ggtgccgacc cgcgatgccg
       61 aactgccgtg gttcgccgaa cgccgcgcgc gcttcgccga gatcggcacg cgcgtgctgg
      121 tgtcggcgcc ggcggcgatc gaacgctgcc gcgacaagcg cgcgttcgcg gacttctgcg
      181 ccgcacacgg ttttgcgacg ccgccggcgg tgacgctgcc ggcagccgca gacgatttcc
      241 cgctgttcgc gaagccgcgc atcggccagg gatcgggggg tgcgcgccgt atcgagtccg
      301 cggccggact ggccgggctc gatgcggagc actggatcgt gcagcgcttc gacggcgggc
      361 ctgaatacac gctcgatgtc tgcgccggct tcgacggcac ggtgctgtcg gtcgtgccgc
      421 gccggcggct gcaggtgctc gccggcgaat cgatcaccgg cgtcacggtc atggacccgg
      481 cgctggtcga actcggcgcg gccgtcgtgc gcgcactcgg cctgatcggc cacgccaccg
      541 tgcagtgctt ccacgacgcc gccggcagcc ggctgatcga ggtcaacccg cgcttcggcg
      601 gcggggccgc gctcggcttc gcggccggcg ccgactcgcc gcgctggctg ctgcgtctgg
      661 cgcgcggcgg cagcatcgag ccgcagctcg gctgccagcg cgacggcctg tacctgctgc
      721 gccacagcca cgacgtgttc gtggcggcgg aggacgtatg ccgctgatgc ccgcgccacc
      781 tgcgcccgcc gtcgtgctgc tcgatctcga cgacacgctg tacgccgagc gcgactggct
      841 gctgagcggc tgccgcgcgg tggccgggtg cgccgaagcg ctgctcgggc gttcggccgc
      901 cgaactctac gactggctgc tgcagcggct gctgaccgag ggccgcgagc gtctgttcga
      961 tgcgctgctc gccgcgcacg ggcgctgcga cccggcgctc agtgcgctgc tcgtgcatgt
     1021 gtttcgcagc caccggccgc ggctgacggc ctttgccgac gtggcgccgg cgctggcggc
     1081 ctggcgtgcg cgcggctgcg ggctcgggct gatcaccgac ggcgtcagct gcgcgcagca
     1141 cgccaagctc gacggcctcg gcctgcgcgc cggcttcgat ctggtgctct gcaccgacga
     1201 cctgcccggg gcagcgcgca agcccgcgcc gctcgccttc cagctcgcgc tcgcgcactt
     1261 cggctgcgcg ccggagcagg ccgtctacat cggcgacgat ccgtccaagg atttcctcgc
     1321 gccgcgcgcg ctcggcatgc gcacgctgcg catcgaccgt cacctgccct ggccgctgca
     1381 gtcgcgccgc gactttccgg acagtcacca ggcacaccgc accatcgaat cgctggcaga
     1441 agcggcggca ccggatgcgt ccggacttgc cgcctgatgc cgcccgtgct ggagaacgtc
     1501 atcatgagtt gcacaggctt tgaaatcgcc ggccgccgga tcggtccgga cgcaccgccg
     1561 ttcgtgatcg ccgagatgtc cggcaaccac aaccagtcgc tgcagcgcgc gctcgccatc
     1621 gtcgatgccg cggccgccgc cggtgcgcac gccatcaagc tgcagaccta taccgccgac
     1681 acgatgacgc tcgacctcga cgagggcgag ttccacatcg atgacccggc gagtctctgg
     1741 cgcggcgagt cgctgtaccg gctctaccgt caggcctaca cgccctggga atggcacgcg
     1801 ccgctgttcg cgcgtgcccg cgagcacggg ctgatcgcct tcagcacgcc cttcgatgcg
     1861 agtgccgtcg atttcctcga cagtctcgac gtgccggcct ggaaggtcgc gtccttcgag
     1921 aacaccgacc tgccgctgat ccggcgcatg gccgccaccg gcaggccgat gatcatctcg
     1981 accggcatgg cgacggtcgg cgaactcgac gagacggtac gcacggcgcg cgccgccggc
     2041 tgccgggacc tcgtgctgct caagtgcacg agcacctatc cggcaacgcc ggccgacacc
     2101 cacctgctga cgctcccgca cctgcgcgcg ctgttcggct gcgaggtcgg cctgtcggat
     2161 cacacgatgg ggctcgggg
//
LOCUS       DAOQJH010000533         2168 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_39467_length_2168_cov_3.656886, whole genome shotgun sequence.
ACCESSION   DAOQJH010000533 DAOQJH010000000
VERSION     DAOQJH010000533.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2168)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2168)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2168
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39467_length_2168_cov_3.656886"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..511
                     /locus_tag="PLQ18_11360"
     CDS             <1..511
                     /locus_tag="PLQ18_11360"
                     /inference="COORDINATES: protein motif:HMM:NF015550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HNE02292.1"
                     /translation="LNATQAAIRAGYSAKTAGRIGQNLVVKSHIASAIAERRQARTER
                     VEIQQDDVLRELAAMGMYDPADIASANISGPTDIAKLPESTRRAIIGWSWDRQGNFVL
                     KLSPKTPSLELLGRHLGMWKDEVTHKGQVALQTIAGASLDSLSPAALDAIESAHAALR
                     SAGVIEPQK"
     gene            508..2019
                     /gene="terL"
                     /locus_tag="PLQ18_11365"
     CDS             508..2019
                     /gene="terL"
                     /locus_tag="PLQ18_11365"
                     /inference="COORDINATES: protein motif:HMM:NF015211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HNE02293.1"
                     /translation="MTVPVAVRAIARLSQWEIEAAQARRGLARFAPMFVPEAVPAAHH
                     LLLIDKLERVTAGEITRLMVFMPPGSAKSTYGSVLWPAWHLGHRPGHCVIGASHTAEL
                     AERFGRRVRGIVADERYSGSAGRPGVFGESASLSGESRAAGRWDTRSRSEYFAAGVGG
                     AITGRRADVAVIDDPVKSREEADSEVVRARVYDWYLADLRTRLKPGAAIVLIMTRWHE
                     DDLAGRILPEKYDGESGVIRARDGEDWTVLALQAIADRDDDPLGRKRGAPIWPQWQSA
                     EVLAQERISQGPRNWESLYQQRPHPREGTLFRTERMLTVEAAPVGLECVRGWDLAATE
                     QIGSADPDYTASVKLGVTPDGMVYILDVRRQQTSPHGVESMICDAAASDGRRVRISLP
                     QDPGQAGKSQIAYLTRKLQGHIVASSPETGSKERRAEPIAAQIEAGNVRMLKAGWNAE
                     FIDELKAFPSGRHDDMVDALSRAYMLLTERAVRPAASPLRQPVRPAGGWAAHT"
BASE COUNT          364 a          741 c          746 g          317 t
ORIGIN      
        1 cctgaacgcg acgcaggcgg cgatcagggc cggctacagc gcgaagacgg ccggccgtat
       61 cggtcagaat ctggtggtaa aaagtcacat cgccagcgcg atcgccgagc gccgccaggc
      121 ccgcaccgag cgcgtcgaaa tccagcagga cgacgtgctg cgcgaactgg ccgcaatggg
      181 catgtacgat cccgctgata tcgccagcgc caatatttcc ggcccgaccg atatcgcgaa
      241 actgccggaa tccacgcgcc gcgcgattat cggctggagt tgggacaggc aaggcaattt
      301 cgttctgaaa ctgtcgccga aaaccccgag cctagaattg ctcgggcggc acttgggcat
      361 gtggaaggac gaagtaacgc ataaagggca agtcgcgctg cagaccatcg ccggcgcctc
      421 gctcgactcg ctatcacccg ccgccctcga cgcaatcgaa tccgcgcacg ccgcgctgcg
      481 atccgccggc gtgatcgagc ctcagaaatg accgttcctg tcgccgtgcg cgccatcgcc
      541 cgcctgtcgc agtgggaaat cgaggccgca caggcccgtc gcggcctcgc gcgtttcgcg
      601 ccgatgttcg tgccggaggc cgtgccggcc gcgcaccatc ttctgctgat cgacaagttg
      661 gagcgcgtca ccgccggcga gatcacgagg ctgatggtgt tcatgccgcc gggcagcgcg
      721 aagtccacct acggctccgt gctctggccg gcgtggcacc tcgggcaccg gcccggtcac
      781 tgcgttatcg gcgcctcaca cacggccgag ctggccgagc gcttcggccg gcgcgtgcgc
      841 ggcatcgtcg cggacgagag atacagcggc agcgctggcc gccccggcgt gttcggcgag
      901 tccgccagcc tgtccggcga gtctcgcgct gccgggcgat gggatacgcg ctcccggtcc
      961 gagtatttcg ctgccggcgt cggcggcgcg atcaccggcc gacgcgccga cgtggccgtg
     1021 atcgacgatc cggtcaagtc gcgcgaagag gctgacagcg aggtcgtgcg cgcgcgtgtc
     1081 tatgactggt acctcgccga cctgcgcacg cgcctgaaac ccggcgcggc tatcgtgctg
     1141 atcatgacgc gctggcacga agacgatctg gccggccgca tcctgccgga gaaatacgac
     1201 ggggaatccg gcgtgatccg ggcgcgtgac ggcgaggact ggaccgtgct cgcgctgcag
     1261 gcgattgctg atcgcgacga tgatccgctc gggcgcaagc gcggggcacc gatctggccg
     1321 cagtggcagt ccgccgaagt gctggcgcag gagcgcatat cgcaggggcc gcgcaactgg
     1381 gaatccctgt accagcagcg cccgcacccg cgcgagggca cgctgttccg gaccgagcgc
     1441 atgctgaccg tcgaggcggc cccggtcggg ctcgaatgcg tgcgcggctg ggatttggcc
     1501 gcaactgagc agatcggcag cgccgaccct gactacacgg ccagcgtgaa gctcggcgtg
     1561 accccggatg gcatggtcta catcctcgac gtgcgccggc agcagaccag tccgcacggc
     1621 gtcgagagca tgatctgcga cgcggcagcc agtgatggcc ggcgtgttcg catcagtctg
     1681 ccgcaggacc ctgggcaagc cggcaagagc cagatcgcct acctgacgcg caagctgcag
     1741 ggccacatcg tggcgtccag tcccgaaacc ggcagcaaag agcggcgcgc ggagccgatc
     1801 gcagcgcaga tcgaggccgg caacgtgcgc atgctcaagg ccggctggaa tgcggagttc
     1861 atcgacgagc tgaaggcgtt cccgtctggc cggcacgacg atatggtcga tgcgctgtcg
     1921 cgcgcctaca tgctgctgac cgagcgcgcc gtgcgtccgg ccgcttctcc actgcgtcag
     1981 ccggtgcgac ccgccggcgg atgggcggcg cacacatgat gacggttccc gcatgggcat
     2041 cctgagcctg ccggcgcccg gcgacaccgg cctgctgcca atcctgtcgg cctcgcaagt
     2101 ggttgccgcg caggatgccg cgatcgagcg cgcccgtccg cagagtccgg cgccggtcgt
     2161 agacaatc
//
LOCUS       DAOQJH010000534         2167 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_39503_length_2167_cov_4.136837, whole genome shotgun sequence.
ACCESSION   DAOQJH010000534 DAOQJH010000000
VERSION     DAOQJH010000534.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39503_length_2167_cov_4.136837"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            114..1076
                     /locus_tag="PLQ18_11370"
     CDS             114..1076
                     /locus_tag="PLQ18_11370"
                     /inference="COORDINATES: protein motif:HMM:NF012981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HNE02294.1"
                     /translation="MDTLPISLILNPGSGSRRDPALAQSILDTLSAGGRQVEWLKPRR
                     GAEIPAMAAKAMANGPRLMVAAGGDGTVNAVAGAVLAHPGSELGVIPLGTFNYFARGL
                     RLPEDPLAAAGIVRDGQTTAISVGSANGRVFLVHASFGLYRSVIEARERHKNLIGRHR
                     LVAAISGLITALRPHPHYRLKLGIGDEAMRRVSSMVYFGANPLQNAELGVGGADTPES
                     GLAVFVLRPMTRWQVLVTTCHALFGHVADSESIEVFHAQEVTVERHRKRINLVLDGEI
                     FHEQSPLVVRWLPDALPVRVAPVIEDDAQALQDDAPPATMEPAA"
     gene            1073..1894
                     /locus_tag="PLQ18_11375"
     CDS             1073..1894
                     /locus_tag="PLQ18_11375"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNE02295.1"
                     /translation="MRTIAHVSDLHFGRIDPAASAALGAALRSLKPDLVIVSGDLTQR
                     ARSEQFREAAEFLAGLATPVLAVPGNHDVPLWHALARFLWPYHGYRRHIAVELEPVHE
                     DAELLVLGLNSARSLAFYGGRLNSEQVGRAVERLRHAGRERLKIVVSHHPLSAPLTAG
                     PEAESVGRGARMYRRLVDAGGEVFLAGHFHHTAHELAEPLATSRRRALLVHAGTATST
                     RRRASEGNGFNLLRYAGGHLSVVHYEHRSQRSTDFVPVTSRDYHRGDYGWEPLAH"
     gene            complement(1950..>2167)
                     /locus_tag="PLQ18_11380"
     CDS             complement(1950..>2167)
                     /locus_tag="PLQ18_11380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02296.1"
                     /translation="HWLAQWAGQFATWITEGREPWFFAHMPDDRDAPALARAFHEELR
                     SRIASLPALPPWPAGRLPQQSGLFGGN"
BASE COUNT          325 a          787 c          733 g          322 t
ORIGIN      
        1 ggcatcggga ccggaatcgg gcttcggtgc gacgggggat ggcaggttcg gattcatcgt
       61 ctggactcct tcgggccgga acttgctgcg ggcatcggct atcactgccc ctcatggata
      121 ccttacccat cagcctgatc ctgaaccccg gctccggttc ccgccgtgac ccggccctgg
      181 cgcagtccat cctcgacacg ctctcggccg gcggccggca ggtcgaatgg ctcaagcccc
      241 ggcggggagc cgagatcccc gccatggcgg cgaaggcgat ggcgaacgga ccacggctga
      301 tggtggccgc cggtggcgat ggcaccgtca acgccgttgc cggcgcggtg ctcgcgcatc
      361 cgggcagcga actcggcgtg atcccgctcg gcaccttcaa ctacttcgca cgcgggctgc
      421 gcctgcccga ggatccgctt gccgcagcgg gcatcgtgcg cgacggtcaa accactgcga
      481 tcagcgtcgg cagcgccaac ggccgggtgt ttctggtgca tgcgagcttc gggctgtacc
      541 gctcggtgat cgaggcgcgc gagcggcaca agaacctgat cggccgtcac cggctggtcg
      601 cggcgatctc gggcctgatc accgcgctgc gtccgcatcc gcactaccgg ctgaagctcg
      661 gcatcggcga cgaggcgatg cgccgggtca gctcgatggt ctacttcggc gccaacccgc
      721 tgcagaacgc cgaactcggc gtcggcggcg ccgacacgcc ggagtcgggt ctggccgtgt
      781 tcgtgctgcg accgatgacg cgctggcagg tgctggtgac gacctgccac gcgctgttcg
      841 gtcatgtcgc cgacagcgag tcgatcgagg tcttccacgc gcaggaagtc acggtcgaac
      901 gtcaccggaa acgcatcaac ctcgtgctcg acggcgagat cttccacgag caatcgccgc
      961 tggtggtgcg ctggctgccc gatgcgctgc cggtgcgcgt cgcccccgtc atcgaagacg
     1021 acgcgcaggc tctgcaggac gacgcaccgc cggcgaccat ggagccggcg gcatgaggac
     1081 gatcgcgcac gtttcggacc tgcacttcgg acgcatcgac ccggccgcat ccgcggccct
     1141 cggtgcggcg ctgcgcagtc tgaaaccgga tctggtcatc gtctccggcg acctgaccca
     1201 gcgtgcgcgc agcgaacagt tccgcgaggc cgccgagttc ctggccggcc tcgccacgcc
     1261 ggtgctggcc gtgcccggca accatgacgt gccgctctgg cacgcgctgg cgcgcttcct
     1321 gtggccgtac cacggctacc ggcggcacat cgccgtcgaa ctcgaaccgg tgcatgagga
     1381 tgccgaactg ctggtgctcg gcctcaacag cgcgcgctcg ctggccttct acggcggacg
     1441 gctgaacagc gagcaggtcg ggcgcgccgt cgagcgcctg cgtcacgccg gtcgcgagcg
     1501 gctgaagatc gtcgtcagcc atcacccgct gtcggcaccg ctcacggccg gtcccgaggc
     1561 cgaatcggtc ggacgtggcg cgcgcatgta ccggcggctg gtcgatgccg gcggcgaggt
     1621 gttcctggcc gggcatttcc atcacaccgc gcacgaactc gccgaaccgc tcgccaccag
     1681 ccgccgccgg gcgctgctcg tgcatgccgg caccgcgacc tcgacgcgcc ggcgcgcgag
     1741 cgaaggcaac ggcttcaacc tgctgcgcta cgccggcggt cacctgagcg tcgtgcatta
     1801 cgaacaccgc tcgcagcgca gcaccgactt cgtgccggtc acgagccgcg actatcaccg
     1861 cggtgactac ggctgggagc cgctcgcgca ctgacggcgt cgtgcccgcg gcccgtcccg
     1921 gcaaaacccg ggcaaggaat gtccggtttt cagttccccc cgaacagccc tgactgctgc
     1981 ggcaggcgcc cggccggcca cggcggcaag gccggcagcg acgcaatccg cgagcgcagc
     2041 tcctcatgaa aggcccgcgc cagcgccggg gcatcgcgat catccggcat gtgcgcaaag
     2101 aaccacggct cgcggccttc cgtgatccag gtcgcgaact gcccggccca ctgcgcaagc
     2161 cagtgcc
//
LOCUS       DAOQJH010000535         2165 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_39598_length_2165_cov_3.615166, whole genome shotgun sequence.
ACCESSION   DAOQJH010000535 DAOQJH010000000
VERSION     DAOQJH010000535.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2165
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39598_length_2165_cov_3.615166"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..744)
                     /locus_tag="PLQ18_11385"
     CDS             complement(<1..744)
                     /locus_tag="PLQ18_11385"
                     /inference="COORDINATES: protein motif:HMM:NF016121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HNE02297.1"
                     /translation="MSFAEFRLLCLPVLRSLSALLLLVACGVAPAAPAAAPWELGRGW
                     FPGADRVAPADGTPPAAAVYRGSELLGYLFQTDQVERIPAYSGKPVNTLVGIDRSGRI
                     AGAQVLEHHEPILLVGIPETRLGEFIAQYVGKRVTDRVKVGAANREGYVGVDAITGAT
                     VTVMVINEGIMRSAHRVAVARGLIGEAAAVQRAPARVKADVYAPADWQQLSGDGSVRT
                     LRLTRADVQRAFDNTAAANVERPTPEQLDE"
     gene            1184..2023
                     /locus_tag="PLQ18_11390"
     CDS             1184..2023
                     /locus_tag="PLQ18_11390"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008248662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HNE02298.1"
                     /translation="MSAAPEFAQPASLPPTSEDYHALTIRSVHWWSPTLFSLVLDRPE
                     SFRFISGQFARLGLPVEGEWVWRAYSICSSVFDEHLEFLSIVVPNGPFTQALKHVQAG
                     DTLYVDKRNFGTLTTDRFRGQKDLWMLATGTGLAPFMSILRDPYTWDTFEHVVLVHSV
                     RDGNELAYLEWLENFNEDEIFGEYAERFRYVKVVTREAVRGAFDKRIPDLVESGLLEQ
                     EVGWEYSPERSAVMLCGNPGMLRQMKTVLQGRGLTVNKKSSPGNILWENGFLPEDNKH
                     ADL"
BASE COUNT          343 a          802 c          689 g          331 t
ORIGIN      
        1 ctcgtcgagc tgctccggcg tcgggcgctc gacgttggcg gcggcggtgt tgtcgaaggc
       61 gcgctgcacg tcggcgcgcg tcagccggag cgtgcgcacc gaaccgtcgc cgctgagctg
      121 ctgccagtcg gccggcgcgt agacatcggc cttcacacgc gccggtgcgc gctgcacggc
      181 cgccgcctcg ccgatcaggc cgcgcgcgac cgcgacccgg tgcgccgagc gcatgatgcc
      241 ctcgttgatg accatgacgg tcacggtcgc gccggtgatc gcatcgacgc cgacgtagcc
      301 ctcgcggttg gcggcgccga ccttgacgcg atcggtcacg cgcttgccga cgtactgcgc
      361 gatgaactcg ccgagccggg tctcggggat gccgaccagc aggatcggct cgtggtgttc
      421 gagcacctgc gcaccggcga tgcggccgct gcggtcgatg ccgaccagcg tgttgaccgg
      481 cttgcccgaa taggccggga tgcgctcgac ctgatcggtc tggaacaggt agccgagcag
      541 ctcgctgccg cggtacacgg ccgcagccgg cggcgtgcca tcggcgggcg caacgcgatc
      601 ggcgcccggg aaccagccgc ggccgagctc ccacggcgcc gccgccggtg cggccggcgc
      661 gacgccgcaa gccacgagca gcagcagcgc gctcaaggag cgcaggaccg gcaggcacag
      721 caggcggaat tcagcgaagg acatggcatc tgcaacggcc accgctcgcg cggagggcag
      781 ccgtgcccgg tcagggtgcg gcgatctgcc gcaggtcggg gcacaccgta gccccctgac
      841 cggccagtat cttgatgatc atcaagccca tgaccattgt gccggccact tacccccgag
      901 ggggcagcca cccgtacttg ccctgcgccg gccggctacc cgcaccgatc aggtgcatct
      961 gccgctacct ctgccatgcc tgcgcatcgc aaccggcccg gcgctgccgg cgcctgccgc
     1021 ttcggagcgc atcggccggg aggcagggcc tcatataaga agattgaata aaaaaatcca
     1081 ttcagattct ttccagcaat ttaatatccg acttatcctc ccggacaatt ttcgcccccc
     1141 gagggcaggc accgcccttc cgtcagccga gaacctgcaa tccatgtccg cagcacccga
     1201 gttcgcccag cccgcctccc tgcccccgac cagtgaggac taccacgccc tgaccatccg
     1261 cagcgtgcac tggtggtcac cgacgctgtt ctcgctggtg ctcgaccgtc cggagtcctt
     1321 ccgcttcatc tccggccagt tcgcccgcct cggcctgccg gtcgaaggcg aatgggtctg
     1381 gcgggcgtac tcgatctgtt cgtcggtctt tgacgagcac ctggaattcc tgtcgatcgt
     1441 cgtgccgaac ggcccgttca cgcaggcgct gaagcacgtg caggccggcg acacgctcta
     1501 cgtcgacaag cgcaacttcg gcacgctgac caccgaccgc ttccgcggcc agaaggatct
     1561 ctggatgctc gcgaccggca ccggcctggc gccgttcatg tcgatcctgc gcgaccccta
     1621 cacctgggac accttcgagc acgtcgtgct ggtgcacagc gtgcgcgacg gcaacgagct
     1681 cgcgtatctc gaatggctcg aaaacttcaa tgaggacgag atcttcggcg agtacgccga
     1741 gcgtttccgc tacgtgaagg tcgtcacgcg cgaggccgtg cgcggcgcct tcgacaagcg
     1801 catccccgac ctcgtcgagt ccggcctgct ggaacaggaa gtcggctggg agtactcgcc
     1861 cgagcgctcg gcggtcatgc tctgcggcaa ccccggcatg ctgcgccaga tgaagacggt
     1921 gctgcagggc cgcggcctga ccgtcaacaa gaagtccagc cccggcaaca tcctctggga
     1981 aaacggcttc ctgcccgagg acaacaagca cgccgatctc tgatccggcg gcatttccgc
     2041 tttcccccga cggccggcac gcgccctgcg tgccggccgt cgcgtttgcg gccacccggc
     2101 agcaagcccc gcccccgcgc ccccgcccgg ctcccgccag ccccgcactc ccgccgccct
     2161 gaacc
//
LOCUS       DAOQJH010000536         2163 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_39671_length_2163_cov_3.764706, whole genome shotgun sequence.
ACCESSION   DAOQJH010000536 DAOQJH010000000
VERSION     DAOQJH010000536.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2163)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2163)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2163
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39671_length_2163_cov_3.764706"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(147..626)
                     /gene="greA"
                     /locus_tag="PLQ18_11395"
     CDS             complement(147..626)
                     /gene="greA"
                     /locus_tag="PLQ18_11395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009116507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HNE02299.1"
                     /translation="MTQKVPMTATGAEKLRAELQELKTVARPKVIEAIAEARAHGDLK
                     ENAEYSAAREQQGFIEGRIKEIEYKLSHAHVIDVTALPRTGKVVFGVTVTLADEDSGD
                     EVRYQIVGEDEADIPSGRISVNSPIARALIGKEEGEVATVQTPGGVRHWEVVAVEHL"
     gene            complement(623..>2163)
                     /gene="carB"
                     /locus_tag="PLQ18_11400"
     CDS             complement(623..>2163)
                     /gene="carB"
                     /locus_tag="PLQ18_11400"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006788013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HNE02300.1"
                     /translation="ILGGGPNRIGQGIEFDYCCVHASFALHEAGFETVMVNCNPETVS
                     TDYDTSDRLYFEPLTLEDVLEIVRVEAPKGVIVQYGGQTPLKLARPLEAAGVPIIGTS
                     PDAIDRAEDRERFQRMIDELALRQPPNRTARDPETAVRLAAEIGYPLVVRPSYVLGGR
                     AMEIVHNEDDLRRYMREAVQVSNDSPVLLDRFLNDAIEVDVDAIADADGDVVIGGIME
                     HIEEAGVHSGDSACSLPPYSLSPLTLDRLRTQVRAMARELGVVGLMNTQFAIQGDDIF
                     VLEVNPRASRTVPYVSKATGRPLAKIAARCMAGRSLAGQGVQGEIVPSYYSVKEAVFP
                     FVKFPGVDPLLGPEMKSTGEVMGVGRSFGEAYAKAQLGAGNRLPASGKAFLSVREVDK
                     VKIVEVARDLVARGFSLVGTRGTAATLNAAGLECAIVNKVGEGRPHIVDMIKNDEISL
                     IVNTTEGKQAIADSFSLRRNALMHKVSYTTTIAAARASVMALEDLGSDRVTRLQDLHK
                     ELQA"
BASE COUNT          321 a          715 c          761 g          366 t
ORIGIN      
        1 atcaggaaga tcgcgagcag cgtcgcgcgg gtcaggcatc ggtacatggc agtgaacctg
       61 cgtcgtcggg gggccgaaac gcgaagggca ggcgcatcgg cctgcccttc ggagtcggga
      121 gagaagcggc gcgcggggac cggcgcctac agatgctcga ccgccacgac ttcccagtgc
      181 cggacgccgc ccggggtctg cacggtggcg acctcgccct cctccttgcc gatcagcgcg
      241 cgtgcgatcg gggaattcac cgagatgcgc ccggacggga tgtcggcctc gtcctcgccg
      301 acgatctgat aacggacctc gtcgccggag tcctcgtcgg cgagcgtgac ggtgacgccg
      361 aacacgacct tgccggtacg cggcagcgcg gtcacgtcga tgacgtgcgc atgcgagagc
      421 ttgtattcga tttccttgat gcggccttcg atgaagccct gctgctcgcg cgcggcggaa
      481 tattcggcgt tttccttcag gtccccgtgt gcgcgcgcct cggcgatcgc ctcgatgact
      541 ttcgggcggg caacggtttt cagttcctgc agttcggccc tgagcttttc tgcgccggtg
      601 gcagtcatcg gcaccttctg ggtcatgcct ggagctcctt gtgcaaatcc tggaggcggg
      661 tgacgcgatc gctgccgagg tcttcgagcg ccatgacgga ggcgcgcgcg gcggcgatcg
      721 tggtggtgta gctgaccttg tgcatcagcg cattgcgtcg cagcgagaac gagtcggcaa
      781 tggcctgctt gccttcggtg gtgttgacga tcagcgagat ctcgtcgttc ttgatcatgt
      841 cgacgatgtg cggccggcct tcgccgacct tgttgacgat ggcgcactcc agcccggcgg
      901 cgttgagcgt cgccgccgtg ccgcgggtgc cgaccagcga gaagccgcgg gcgaccagat
      961 cgcgcgccac ctcgacgatc ttgaccttgt cgacctcgcg caccgacagg aaggccttgc
     1021 cggaggccgg caggcggttg ccggcgccga gctgggcctt ggcgtaggcc tcgccgaagc
     1081 tgcgcccgac gcccatgacc tcgccggtcg acttcatctc ggggccgagc agcggatcga
     1141 cgcccgggaa cttcacgaac gggaacacgg cttccttgac cgaatagtac gagggcacga
     1201 tctcgccctg cacgccctgc ccggccagcg agcgcccggc catgcagcgc gcggcgatct
     1261 tcgcgagcgg ccggccggtg gccttggaaa catagggcac ggtgcgcgag gcgcgcggat
     1321 tgacttcgag cacgaagatg tcgtcgccct ggatcgcgaa ctgcgtgttc atcagcccga
     1381 cgacgccgag ctcgcgcgcc atcgcccgca cctgcgtgcg cagccggtcg agcgtcagcg
     1441 gcgacagcga gtacggcggc agcgagcagg ccgaatcgcc cgagtgcacg ccggcctcct
     1501 cgatgtgctc catgatgccg ccgatgacga catcgccgtc ggcgtcggcg atcgcgtcga
     1561 catcgacctc gatcgcgtcg ttcaggaagc gatcgagcag caccggactg tcattgctga
     1621 cctgcacggc ctcgcgcatg tagcggcgca ggtcgtcctc gttgtggacg atctccatcg
     1681 cccggccgcc gagcacgtag gacgggcgca cgaccagcgg atagccgatc tcggcggcca
     1741 ggcgcacggc ggtctcggga tcgcgcgcgg tgcggttcgg cggctggcgc agcgccagtt
     1801 cgtcgatcat gcgctggaag cgctcgcggt cctcggcgcg gtcgatggca tcggggctgg
     1861 tgccgatgat cggcacgccg gcggcttcga gcggacgcgc gagcttcagc ggcgtctggc
     1921 cgccgtactg cacgatcacg cccttcggcg cctcgacgcg cacgatctcc agcacgtcct
     1981 ccagtgtcag cggctcgaag tacaggcggt ccgaggtgtc gtagtcggtc gagacggtct
     2041 cggggttgca gttgaccatc accgtctcga agccggcctc gtgcagggcg aagctggcgt
     2101 ggacgcagca gtagtcgaac tcgatgccct ggccgatccg gttggggccg ccgccgagga
     2161 tga
//
LOCUS       DAOQJH010000537         2161 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_39750_length_2161_cov_2.477683, whole genome shotgun sequence.
ACCESSION   DAOQJH010000537 DAOQJH010000000
VERSION     DAOQJH010000537.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39750_length_2161_cov_2.477683"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2044)
                     /locus_tag="PLQ18_11405"
     CDS             complement(<1..2044)
                     /locus_tag="PLQ18_11405"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HNE02301.1"
                     /translation="MLGHNLLDHDWPLLRAQAPQAGLLRLPVLDTLYLSPLAFPENPY
                     HALVKDYKLVRDSVNDPVADARLAGRVFLEQVAAFAARAQGGPDWADLLRYAWTDAAA
                     GRDGLSPTAFLWLWERLGGRPPADPGALIERLIDARTCRSRRSAALQPLLMQPGLRPL
                     LGYLVTWLMVAGGNSVLPAWLRHRFPALPEAVRRLRDEPCSDPACAWCRDAHDPRGQL
                     ERWFGFPDFRAEPATAEGGSLQRAIVAAGLGHGSLLGILPTGGGKSLCYQVPALARYR
                     NRGALTVVISPLRALMKDQVDGLNRRVGFELCGALYGDLTPPERGALIERVQLGDIAV
                     LYVAPEQFRNASFRSLLESREIGAWVFDEAHCLSQWGHDFRPDYLYCARFIREFGERH
                     KLPLAPVSAVTATAKPDVIDEIRRHFRQELGLELTLYAGGVERDNLRFEVRAVTSASK
                     PAQVLQLLRERLEPAPGAAIVYCAKQDSTVEFARFLDSEGWAAEAFHGGMKAPDKRSV
                     QERFLAGELRVICATNAFGMGIDKPDVRLVIHADAPSSLEHYLQEAGRAGRDREPADC
                     VLLYAPEDIERQFRWGARSRLSRQDIAGILRALRRMQQKRDADEPVVVTAGELLRSDS
                     LLTGFETEDADTRVRTALAWLEKAQLVERNENRTGVFQGRPSVGSMAEARAKLDALD"
BASE COUNT          323 a          783 c          778 g          277 t
ORIGIN      
        1 gatcgagcgc atcgagcttc gcgcgcgcct cggccatcga gccgaccgag ggccggccct
       61 ggaacacgcc ggtgcggttc tcgttgcgct cgaccagttg cgccttctcc agccacgcga
      121 gcgcggtgcg cacgcgggtg tcggcatctt cggtctcgaa gccggtgagc aggctgtcgc
      181 tgcgcagcag ctcgccggcg gtgacgacga ccggctcgtc ggcatcgcgc ttctgctgca
      241 tgcggcgcag cgcgcgcagg atgccggcga tgtcctggcg gctcaggcgg ctgcgcgcgc
      301 cccagcgaaa ctgccgttcg atgtcctcgg gcgcgtacag cagcacgcag tcggccggct
      361 cgcgatcgcg cccggcgcgg ccggcctcct gcaggtagtg ctccagcgag gacggcgcat
      421 cggcgtggat gacgaggcgc acgtcgggct tgtcgatgcc catgccgaag gcattggtcg
      481 cgcagatcac gcgcagctcg ccggcgagga agcgctcctg caccgaacgc ttgtccggtg
      541 ccttcatgcc gccgtgaaag gcttcggccg cccagccctc gctgtcgagg aagcgggcga
      601 actcgacggt gctgtcctgc ttcgcgcagt agacgatcgc cgcgcccggc gcgggttcga
      661 ggcgctcgcg cagcagctgc agcacctgcg ccggcttgct ggcactcgtg accgcgcgca
      721 cctcgaagcg caggttgtcg cgttcgacgc cgccggcgta cagcgtcagc tccagcccga
      781 gttcctgccg gaagtggcgg cgaatctcgt cgatgacatc gggcttggcg gtggcggtga
      841 cggcgctgac cggcgccagc ggcagtttgt gccgctcgcc gaactcgcgg atgaagcgcg
      901 cgcagtacag gtaatcgggc cggaagtcgt ggccccactg cgacaggcag tgcgcctcgt
      961 cgaacaccca ggcgccgatc tcgcggcttt cgaggaggct gcggaagctc gcgttgcgaa
     1021 actgctcggg cgcgacgtac agcacggcga tgtcgccgag ctgcacgcgc tcgatcagcg
     1081 caccgcgctc gggtggcgtc aggtcgccgt acagcgcgcc gcagagctcg aagccgacgc
     1141 gccggttcag gccatcgacc tgatccttca tcagcgcgcg cagcggcgag atcaccaccg
     1201 tcagcgcgcc gcggttgcgg taacgcgcga gcgccggcac ctgatagcac agcgacttgc
     1261 cgccgccggt cggcaggatg ccgagcagcg agccgtgccc gagcccggca gcgacgatcg
     1321 cgcgctgcag gctgccgccc tcggccgtgg ccggctcggc gcggaagtcc ggaaaaccga
     1381 accagcgttc gagctgcccg cgcgggtcgt gcgcgtcgcg acaccaggcg caggccgggt
     1441 cgctgcaggg ctcgtcgcgc aggcggcgca cggcctccgg cagcgccgga aaccggtgcc
     1501 gcagccaggc cggcagcacc gaattgccac cggcgaccat cagccaggtg acgagataac
     1561 cgagcagcgg ccgcagcccc ggctgcatca gcagcggctg cagcgccgcc gagcgccggc
     1621 tccggcaggt gcgggcgtcg atcaggcgct cgatcagcgc gccgggatcg gccggcggcc
     1681 ggccgccgag gcgctcccag agccacagga aagccgtcgg cgacaggccg tcgcgcccgg
     1741 ccgccgcatc ggtccacgca tagcgcagca gatcggccca gtccggcccg ccctgcgcgc
     1801 gcgccgcaaa ggccgcgacc tgttcgagga acacccgacc ggccagccgg gcatcggcaa
     1861 ccgggtcgtt gacgctgtcg cgcaccagct tgtagtcctt gaccagcgcg tgatacgggt
     1921 tttccgggaa ggcgagcggc gacagataca gcgtgtcgag caccggcagg cgcagcagcc
     1981 cggcctgcgg cgcctgcgcg cgcagcagcg gccagtcgtg atcgagcagg ttgtgcccga
     2041 gcacgagccg cgcaccgtgc gcgaacccgg ccagctccgc cagcacggca ccgacctgca
     2101 gccggccttc gcgcaaaaaa cactggccgc ggaattcggc gccgatcgcg aggatgcgcc
     2161 c
//
LOCUS       DAOQJH010000538         2157 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_39858_length_2157_cov_4.705519, whole genome shotgun sequence.
ACCESSION   DAOQJH010000538 DAOQJH010000000
VERSION     DAOQJH010000538.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2157
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39858_length_2157_cov_4.705519"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            305..>2157
                     /locus_tag="PLQ18_11410"
     CDS             305..>2157
                     /locus_tag="PLQ18_11410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003455140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HNE02302.1"
                     /translation="MALAHVSLDDKYALESGRGYLDGTQALVRLLLMQRARDRLHGLN
                     TAGFVSGYRGSPLGGLDQALWGAKRFLERDGIVFKPGLNEDLAATAVWGTQQVGLFPG
                     ARHEGVFAMWYGKGPGVDRSGDVFKHGNLFGSAAKGGVLAIAGDDHASKSSTLPHQSE
                     YAFMDAMIPVLNPAGVQEILDLGVFGWELSRYCGAWVAMKTIAETVDSSASVTVDPER
                     MHIVLPEGFEFPPEGVHGRWPTRPLEQEFIHQKYRLYAALAFVRANRLDRIVLDSDAP
                     RFGIVTTGKSYLDVMQALDDLGIDAAEAGRIGLRVYKVGMSWPLERDGVRAFAQGLEE
                     ILVVEEKRAVIENQFKEQLYNWREDVRPRVIGKFDEQREWILPSTGELTPAQIARVIA
                     RRIGRFYTSKRIEQRLAFLEAKEAALAKPRPTLDRVPHFCSGCPHNTSTRVPEGSRAM
                     AGIGCHYMVTWMDRATDTFTQMGGEGVTWIGQAPFTDTPHVFQNLGDGTYFHSGLLAI
                     RAAIAAQVNITYKILYNDAVAMTGGQHVDGSLSVAQITHQLYGEGVRRLAVVSDEPGK
                     YRNRGAFADGVTFHHRDALDALQRELRSVPGTSVLIYDQTCAAEKRRRRKRG"
BASE COUNT          333 a          753 c          757 g          314 t
ORIGIN      
        1 gcctcgccgg cgctgcgcgc tgcccacggg gccgccggcc gggcgcgcat cgaggcgcag
       61 ttcagtctgg aggcgatggt cgcgcgctac gcagccgtct acgagcgcgc gctggccggc
      121 tgaggcgtga ccggtctgac cattgaccgg ctgatccggc cttcctaccc tcggcaacag
      181 gctcgcccca caagggtctg accgcctgta cgcaccggct gccccgtccg cgtcccgcgc
      241 gacccaccgt cgcccgcagg atgcgcagcc ggcaccgctt gaagccgaga tgggagatcc
      301 ccgcatggcc ctcgcccacg tcagtcttga cgacaagtac gcgctcgaat cggggcgggg
      361 gtatctggac ggcacccagg ccctggtgcg cctgctgctg atgcagcgcg cccgcgaccg
      421 cctgcacggc ctgaacaccg ccggcttcgt ctccggctac cgcggctcgc cgctcggcgg
      481 cctcgatcag gcgctctggg gcgcgaagcg gtttctggaa cgcgacggca tcgtgttcaa
      541 gcccgggctg aacgaggatc tggccgcgac cgcggtctgg ggcacgcagc aggtcgggct
      601 gtttccgggc gcaaggcacg agggcgtgtt cgcgatgtgg tacggcaagg ggccgggggt
      661 ggaccgttcc ggcgacgtgt tcaagcacgg caacctgttc ggcagcgccg ccaagggcgg
      721 cgtgctcgcg atcgccggcg acgatcacgc ctcgaagtcc tcgacgctgc cgcaccagag
      781 cgaatacgcg ttcatggacg cgatgatccc ggtgctgaat ccggccggcg tgcaggagat
      841 cctcgatctc ggtgtgttcg gctgggagct gtcgcgctac tgcggcgcct gggtggcgat
      901 gaagaccatc gccgagacgg tcgactcgtc ggcgagcgtc accgtcgatc cggagcgcat
      961 gcacatcgtg ctgccggagg gctttgaatt cccgcccgag ggcgtgcacg ggcgctggcc
     1021 gacccggccg ctggagcagg agttcatcca ccagaagtac cggctgtacg cggcgctcgc
     1081 cttcgtgcgc gccaaccggc tcgatcgcat cgtgctcgac agcgacgcgc cgcgcttcgg
     1141 catcgtcacg accggcaaga gctatctcga cgtgatgcag gcgctcgatg atctcggcat
     1201 cgacgcggcc gaggccgggc gcatcggcct gcgcgtctac aaggtcggca tgagctggcc
     1261 gctggagcgc gacggcgtgc gcgcgtttgc gcaggggctg gaggagatcc tggtcgtcga
     1321 ggaaaagcgc gcggtcatcg agaaccagtt caaggagcag ctctacaact ggcgcgagga
     1381 cgtgcgcccg cgcgtgatcg gcaagttcga cgagcagcgc gaatggatcc tgccctcgac
     1441 cggcgagctg acgccggcgc agatcgcgcg cgtgatcgcg cggcgcatcg ggcgcttcta
     1501 cacctcgaag cgcatcgagc agcggctggc cttcctggag gcgaaggagg ccgcgctcgc
     1561 gaaaccgcgg ccgacgctcg atcgcgtgcc gcacttctgc tcgggctgcc cgcacaacac
     1621 ctcgacgcgc gtgcccgaag gcagccgggc gatggccggc atcggctgtc actacatggt
     1681 cacgtggatg gaccgtgcga ccgacacctt cacgcagatg ggcggggagg gcgtgacctg
     1741 gatcgggcag gcgccgttca ccgacacgcc gcacgtgttc cagaacctcg gtgacggcac
     1801 ctacttccac tcggggctgc tcgcgatccg cgccgcgatc gccgcgcagg tcaacatcac
     1861 ctacaagatc ctctacaacg atgcggtcgc gatgaccggc ggccagcacg tcgacggctc
     1921 gctgagcgtc gcgcagatca cgcaccagct atacggcgag ggcgtgcgcc gcctcgccgt
     1981 ggtctccgac gagcccggca agtaccgcaa ccgcggtgcc ttcgccgacg gggtcacctt
     2041 ccaccaccgc gatgcgctcg atgcgctgca gcgcgagctg cgcagcgtgc ccggcaccag
     2101 cgtgctgatc tacgaccaga cctgcgcggc cgagaagcgc cgccggcgca agcgcgg
//
LOCUS       DAOQJH010000539         2150 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_40084_length_2150_cov_4.136516, whole genome shotgun sequence.
ACCESSION   DAOQJH010000539 DAOQJH010000000
VERSION     DAOQJH010000539.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2150)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2150)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2150
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40084_length_2150_cov_4.136516"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..146)
                     /locus_tag="PLQ18_11415"
     CDS             complement(<1..146)
                     /locus_tag="PLQ18_11415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637355.1"
                     /note="GTPase; similar structure to tubulin; forms
                     ring-shaped polymers at the site of cell division; other
                     proteins such as FtsA, ZipA, and ZapA, interact with and
                     regulate FtsZ function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HNE02303.1"
                     /translation="MFEIIETPHQEAIIKVIGIGGGGGNAVQHMVTSHTIEGVDFYCA
                     NTDA"
     gene            complement(289..1521)
                     /gene="ftsA"
                     /locus_tag="PLQ18_11420"
     CDS             complement(289..1521)
                     /gene="ftsA"
                     /locus_tag="PLQ18_11420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007040345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HNE02304.1"
                     /translation="MSRKNEKKMVVGLDIGTSKVVALVGEAAADGSMEVIGVGMHPSR
                     GLKKGVVVNIESTVQSIQHAIEEAERTAGCQIHSVHTGIAGSHIRSLNSHGIVPIRDK
                     EVTQEDIDRVIEAAGAVAIPTDQKVLHILAQEFLIDHQEGIREPIGMSGVRLEARVHM
                     VTGAVSAAQNIVKCVERCSLEVDDIVLQPLASARAVLTEDERELGVCLVDIGGGTTDL
                     AVFVNGAIQHSAVIPIAGDQVTNDIAVAFRTPTQFAESLKIQHATCLTALAPADQEID
                     VPSVGERAPRRLSMQTLAEVVEARYEELFGLVQAELRRSGFEDMLAAGIVLTGGSSRI
                     RGAVELAEEIFHVPVRAGTSLAVTGLQEVLANPACSTAVGLLLVGAEARGPVRTGPLR
                     ENRQGLFARMKAWFQGNF"
     gene            complement(1550..>2150)
                     /locus_tag="PLQ18_11425"
     CDS             complement(1550..>2150)
                     /locus_tag="PLQ18_11425"
                     /inference="COORDINATES: protein motif:HMM:NF015739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HNE02305.1"
                     /translation="DMGSLHAAFADNPWVQSVAVSRRWPDTVEVRFVERVPFAHWGRD
                     EMIDTGAARFRPQKIREPHEWPNFDAPDGHEALLMRVYGDTGRALAPLGLKVVRVQQD
                     ARGAWTLTLDTGLELRLGREAYAERLARFIEVWPRVLAAQAERIAGVDLRYLNGFAVR
                     WKAPSAEPGTHGRTRGAPIATAPATARSEDRVFTGHEAG"
BASE COUNT          324 a          769 c          715 g          342 t
ORIGIN      
        1 tgcgcgtcgg tgttcgcgca gtagaagtcg acaccctcga tcgtgtggct ggtgaccatg
       61 tgctgcacgg cattgccgcc gccgccaccg atgccgatga ccttgatgat cgcttcctgg
      121 tgcggtgtct cgatgatttc gaacatgtct tcgtctcccc gatgggcctg accgccccgt
      181 caggccggtt gcgtccggtc acccggtccg cgccagccgg cgacgcagca gcctctgcgc
      241 caccgatgca aaaatcactg aaaaaacaga aacaaaaaca gaaacaaatc agaaattgcc
      301 ctggaaccac gccttcatgc gcgcaaacag cccctggcgg ttctcgcgca gcgggccggt
      361 gcgcaccggc ccgcgtgcct cggcaccgac cagcagcagg ccgaccgccg tcgagcaggc
      421 cggattggcc agcacctcct gcagaccggt gaccgccagc gaggtgcccg cgcgcaccgg
      481 cacgtggaag atctcctcgg ccagctcgac cgcaccgcgg atgcgcgagc tgccgccggt
      541 cagcacgatg ccggcggcga gcatgtcctc gaagccgctg cggcgcagct cggcctgcac
      601 cagcccgaac agctcctcgt agcgggcctc gaccacctcg gcgagcgtct gcatcgacag
      661 ccgccgcggt gcgcgctcgc cgacgctcgg cacgtcgatc tcctgatcgg ccggcgccag
      721 cgccgtcagg caggtcgcgt gctggatctt caggctctcg gcgaactgcg tcggcgtgcg
      781 gaaggccacg gcgatgtcgt tggtgacctg gtcaccggca atcgggatca ctgccgagtg
      841 ctggatcgcg ccgttgacga acaccgcgag atcggtcgtg ccgccgccga tgtcgaccag
      901 gcagaccccg agttcgcgct cgtcctcggt cagcaccgca cgcgccgagg cgagcggctg
      961 cagcacgatg tcgtcgacct ccagcgagca gcgctcgacg cacttgacga tgttctgcgc
     1021 ggcgctgacc gcgccggtga ccatgtgcac gcgcgcctcc aggcgcacgc cggacatgcc
     1081 gatcggctcg cggatgcctt cctggtgatc gatcaggaac tcctgcgcga ggatgtgcag
     1141 gaccttctga tcggtcggga tcgcgacggc gccggcggcc tcgatgacgc ggtcgatgtc
     1201 ctcctgcgtc acttccttgt cgcggatcgg cacgatgccg tgcgagttca ggctgcggat
     1261 atggctgccg gcgatgccgg tgtgcacgga gtggatctgg cagccggcgg tgcgctcggc
     1321 ttcctcgatc gcgtgctgga tcgactgcac ggtcgactcg atgttgacga cgacgccctt
     1381 cttcaggccg cgcgacggat gcatgccgac gccgatgacc tccatcgagc catccgccgc
     1441 ggcctcaccg accagcgcca cgaccttgga cgtgccgatg tcgaggccga ccaccatttt
     1501 cttttcgttc ttgcgcgaca taaaaggatg cgttcccggt ttccggcgat cagccggctt
     1561 cgtgcccggt gaagacccga tcctcgctgc gcgccgtggc cggcgcggtg gcgatcgggg
     1621 ctccccgggt tctgccatgg gtgccgggct ccgcggacgg cgccttccag cgcaccgcga
     1681 aaccgttcag gtaccggaga tccaccccgg cgatgcgttc ggcctgggca gccagcacgc
     1741 gcggccagac ctcgatgaag cgggcgaggc gctcggcata ggcctcgcgg ccgagccgca
     1801 gctccagccc ggtgtcgagg gtcagcgtcc aggcgccgcg cgcatcctgc tgcacgcgca
     1861 cgaccttgag ccccagcggt gccagcgcac ggccggtgtc gccgtagacg cgcatcagca
     1921 gcgcctcgtg tccgtcgggc gcgtcgaagt tcggccactc gtgcggctcg cggatcttct
     1981 gcggccggaa gcgcgcggcc ccggtgtcga tcatctcgtc cctgccccag tgcgcgaacg
     2041 gcacgcgctc gacgaagcgc acctcaacgg tgtccggcca gcggcggctg accgcgaccg
     2101 actgcaccca ggggttgtcg gcgaaggccg cgtgcaggct gcccatgtcg
//
LOCUS       DAOQJH010000540         2146 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_40219_length_2146_cov_4.546628, whole genome shotgun sequence.
ACCESSION   DAOQJH010000540 DAOQJH010000000
VERSION     DAOQJH010000540.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2146
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40219_length_2146_cov_4.546628"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..744
                     /locus_tag="PLQ18_11430"
     CDS             <1..744
                     /locus_tag="PLQ18_11430"
                     /inference="COORDINATES: protein motif:HMM:NF023330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3422 domain-containing protein"
                     /protein_id="HNE02306.1"
                     /translation="VADHGLSPRHAGRLVQRLLEIETYRMLAMLGFPNARRSSQELNG
                     VERRLAEITGRMTGNEAPDAEAKLLTELSDLAAEVERGAATSTYRFAASRAYRTLVER
                     RIEELREERIEGLQTLEEFMERRLAPAMRTVEAVRERQSQLSERVARATGLLRTRVDV
                     TLQQQNQALLASMNRRADLQLRLQETVEGLSVVVLSYYLVSLLGYLVKALSKAGLPLS
                     PEIVSGAAVPVALAALTWKIGRMRKALAH"
     gene            complement(774..1013)
                     /locus_tag="PLQ18_11435"
     CDS             complement(774..1013)
                     /locus_tag="PLQ18_11435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008100386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNE02307.1"
                     /translation="MNRRKIQILGSGCQKCRKLAEYATQAADELGLDWELEKITDPAR
                     IAGFGVMQTPALAVDGVVKLAGHLAGVAELKRLLG"
     gene            complement(1049..2080)
                     /locus_tag="PLQ18_11440"
     CDS             complement(1049..2080)
                     /locus_tag="PLQ18_11440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018413472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HNE02308.1"
                     /translation="MQARLRASGPSLIAVAGAVLGLFVYERLPALAAALAAWLPFAPD
                     SRAGAAIGFFLYEVPKVLLLLCAVVFVMGIVRSYCSPERTRALLAGRRGGLGNALAAA
                     LGVVTPFCSCSAVPLFIGFLGAGVPLGVTLSFLIAAPMVNEIALGLLFALFGWRIAAL
                     YLALGLTVALGAGWIIGRIGMEGSVEDWVRTCPATTAAAGLSGPVGWNAHLGAGSAAV
                     RDIVGRVWPYLLAGIAVGAGIHGYVPTDFMAALMGREAWWSVPLAVALGVPMYSNAAG
                     IVPVVEALLAKGAALGTALAFMMSVVALSLPELVILRKVLKPRLIAVFTGVVATGILL
                     VGYVFNLVL"
BASE COUNT          352 a          760 c          768 g          266 t
ORIGIN      
        1 gtcgccgatc acggcctgtc gccgcgtcac gcagggcggc tggtgcagcg cctgctggag
       61 atcgagacct accggatgct ggcgatgctc ggcttcccga atgcccggcg cagttcgcag
      121 gagctcaacg gcgtcgaacg ccggctggcc gagatcaccg gacgcatgac cggcaacgag
      181 gccccggacg ccgaggcgaa gctgctgacc gagctctccg acctcgccgc cgaggtcgag
      241 cgcggggcgg cgacctcgac ctaccgcttc gcggccagcc gtgcctaccg cacgctggtc
      301 gagcggcgca tcgaggagct gcgcgaggag cgcatcgagg gtctgcagac gctggaggag
      361 ttcatggagc ggcggctggc gccggcgatg cgcacggtcg aggccgtgcg cgagcgccag
      421 agccagctgt ccgagcgcgt cgcgcgggcg accgggctgc tgcgcacgcg cgtcgacgtg
      481 acgctgcagc agcagaacca ggcgctgctc gcgtcgatga accgccgcgc cgacctgcag
      541 ctgcggctgc aggagacggt cgagggcttg tcggtggtcg tgctcagcta ctacctggtc
      601 agcctgctcg gctatctggt caaggcgctg tcgaaggccg gactgccgct gagcccggag
      661 atcgtctccg gggccgcggt gccggtcgcg ctggcggcgc tgacctggaa gatcggccgc
      721 atgcgcaagg cgctcgcgca ctgatgcccc ctccggccct tcgtcgcgga tcctcagccg
      781 agcaggcgct tcagctcggc gacaccggcg aggtggccgg cgagcttcac gacgccgtcg
      841 acggcgaggg ccggggtctg catcacgccg aagccggcga tgcgcgccgg atcggtgatc
      901 ttctccagct cccagtcgag gcccagctcg tcggcggcct gcgtcgcgta ttcggcgagc
      961 ttgcggcatt tctggcagcc gctgccgagg atctggatct tgcgtcggtt catggcaggc
     1021 ctccgcagcc ggtcggtgac cggcctcgtc acagcacgag attgaacacg tagccgacca
     1081 gcaggatgcc ggttgcgacc acaccggtga acaccgcgat cagtcgcggt ttcagcacct
     1141 tgcgcaggat caccagctcg ggcagcgaca gcgcgacgac cgacatcatg aacgccagtg
     1201 ccgtgccgag ggccgcgccc ttggcgagca gcgcctcgac caccggcacg atgccggcgg
     1261 cattcgagta catcggcacg ccgagcgcga cggcgagcgg cacgctccac cacgcctcgc
     1321 ggcccatcag cgccgccatg aagtcggtcg gcacgtagcc gtggatgccg gcgccgaccg
     1381 cgatgccggc gaggaggtaa ggccagaccc gcccgacgat gtcgcgcacg gcggcgctgc
     1441 cggcgccgag atgtgcattc cagccgaccg ggccggaaag accggccgcg gcggtcgtgg
     1501 ccgggcaagt gcgcacccag tcctcgacgc tgccctccat gccgatgcga ccgatgatcc
     1561 agcctgcccc gagcgcgacc gtcaggccga gcgccagata cagcgccgcg atccgccagc
     1621 cgaacagcgc gaacagcagg ccgagcgcga tctcgttgac catcggtgcg gcgatcagga
     1681 acgacagggt cacgccgagc ggcacgccgg ccccgaggaa gccgatgaac agcggcacgg
     1741 ccgagcacga gcagaacggc gtgaccacgc cgagtgcggc ggcgagggca ttgccgaggc
     1801 cgccgcgtcg cccggcgagc agcgcccgcg tgcgctcggg cgagcagtac gagcgcacga
     1861 tgcccatcac gaacacgacg gcgcacagca gcagcaggac cttcggcact tcgtacagga
     1921 agaagccgat ggccgccccg gcccggctgt cgggcgcaaa cggcagccag gcagccaggg
     1981 ccgcggcgag tgccggcagc cgctcgtaca cgaacagccc cagcacggcg ccggccacgg
     2041 cgatcaggct cgggccgctg gcgcgcagtc gcgcctgcat cgacgacgga acggacggaa
     2101 cggtctgcat cgcggcgctc ctgcatttgt ttcgatatcc atagaa
//
LOCUS       DAOQJH010000541         2145 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_40269_length_2145_cov_2.910048, whole genome shotgun sequence.
ACCESSION   DAOQJH010000541 DAOQJH010000000
VERSION     DAOQJH010000541.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2145)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2145)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2145
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40269_length_2145_cov_2.910048"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="PLQ18_11445"
     CDS             <1..225
                     /locus_tag="PLQ18_11445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013031176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNE02309.1"
                     /translation="CVACHGADGNSASGTFPKLAGQHADYLVKALEDYHSGARKNPMM
                     APMAQNLTAQDRLDLAAWFSSQPQGLSVVK"
     gene            357..1988
                     /locus_tag="PLQ18_11450"
     CDS             357..1988
                     /locus_tag="PLQ18_11450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007426589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tannase/feruloyl esterase family alpha/beta
                     hydrolase"
                     /protein_id="HNE02310.1"
                     /translation="MNQKWIAGAVVALIAGLSPVAFAATAAEPVPEDFARRCAALAQL
                     ALPLTDPLTANVVGATSAQLPGQTVHLPAYCRVRAVVRPAIHVEVRLPVDSAWNGRYF
                     QSGCGGLCGDVLTDYPHLFNSSLPGLARGYATATSDTGHAGSNVDGRWGYASPQAEED
                     FAYRSIGATATLAKAVIRQFYAPRTAVPSIFSGCSTGGRLAVKAALKYPNEFNGIVAG
                     APGLDYPGLVGTAFAWLERANTGPDGGRVFNAGNTDKAKLIYETTLAQCDGLDGVVDG
                     SIADPRRCRPDLSALACGLADGPQCLSSVELRTLELWRRGAQDRFGRPLYPGGVPEGS
                     ELYWGLWLTGAPAYAAAFPNGFVPPIALDFLRYLAFETDPGPAYQLTDFDFDRDPARM
                     SASAALLNADDPDLGAFQKAGGRMLMYHGWADPLVTPYKSVDYFEAVAARAGGSAAAD
                     GFVRLFMIPGMDHCGVQSAASPGPGVDERSIDPLTALEHWLFDGSAPDTLTMHAYGPD
                     NQTVAWTRPICRFPQRTVLKDGAGDWHDPASWTCR"
BASE COUNT          313 a          802 c          708 g          322 t
ORIGIN      
        1 tgcgtcgcct gccacggcgc cgacggcaac tccgcgtccg gcaccttccc gaagctcgcc
       61 ggccagcacg ccgactatct ggtcaaggcg ctggaggact accactccgg cgcgcgcaag
      121 aacccgatga tggcgccgat ggcgcagaac ctgacggcgc aggaccgcct cgacctcgcc
      181 gcctggttct ccagccagcc gcaggggctg tcggtcgtca agtaagcagc caggccggcg
      241 gctttggtct gaccagaacc gcatcccctg ccccgtccgt ggcagctctt cccggcctcg
      301 cataggctcg caatgttgcc tcgcaacaat gcaaggaacc gccaggagag ctgccgatga
      361 accagaagtg gattgcaggc gcggtcgtcg cgctcatcgc cggactgtcg ccggtcgcgt
      421 tcgccgcgac cgcagcggaa ccggtgcccg aggacttcgc gcggcgctgc gccgcgctcg
      481 cccagctggc gctgccgctg accgatccgc tgacggcgaa cgtcgtcggc gccacctccg
      541 cccagctgcc cggccagacc gtccacctgc cggcctactg ccgcgtgcgc gccgtcgtgc
      601 gcccggccat ccacgtcgag gtgcgtctgc cggtcgattc ggcgtggaac ggccgttact
      661 tccagtccgg ctgcggcggc ctctgcggcg acgtgctgac cgactacccg cacctgttca
      721 actccagcct gccggggctc gcgcgcggct atgcgaccgc caccagcgac accggccacg
      781 ccggcagcaa cgtcgacggg cgctggggct acgccagtcc gcaggccgag gaggacttcg
      841 cgtaccgctc gatcggcgcc accgcgacgc tcgccaaggc cgtgatccgc cagttctacg
      901 cgccgaggac tgcggtgccg tcgatcttct ccggctgctc gaccggcggg cggctggcgg
      961 tcaaggcggc gctgaaatac ccgaacgagt tcaatggcat cgtcgccgga gcgcccggcc
     1021 tcgattaccc cggcctggtc ggcaccgcct tcgcgtggct ggagcgcgcc aacaccggcc
     1081 cggacggcgg gcgcgtgttc aacgccggca acaccgacaa ggcgaagctg atctacgaga
     1141 ccacgctcgc gcagtgcgac gggctcgatg gcgtcgtcga cggctcgatc gctgatccgc
     1201 gccgctgcag gccggatctg tccgccctcg cctgcggcct ggccgacggc ccgcagtgcc
     1261 tgagcagcgt cgaactgcgc acgctcgaac tctggcgtcg cggcgcgcag gaccgcttcg
     1321 gccggccgct gtatccgggc ggcgtgccgg aaggctcgga gctgtactgg ggcctgtggc
     1381 tgaccggcgc cccggcctat gccgcggcct tcccgaacgg cttcgtgccg ccgatcgcgc
     1441 tcgacttcct gcgctatctg gccttcgaga ccgacccggg accggcctat cagctcaccg
     1501 acttcgactt cgaccgtgac ccggcgcgca tgagcgcctc ggcggcactg ctgaacgccg
     1561 atgatccgga cctcggtgcc ttccagaagg ccggcggccg catgctgatg tatcacggct
     1621 gggccgatcc gctggtcacg ccgtacaaga gcgtcgatta cttcgaggcg gtcgccgccc
     1681 gggccggcgg cagcgccgcg gccgacggct tcgtgcgcct gttcatgatc ccgggcatgg
     1741 atcactgcgg cgtgcagtcg gccgccagcc ccggtcccgg cgtcgacgag cgcagcatcg
     1801 atccgctgac cgcgctcgaa cactggctgt tcgacggcag cgccccggac acgctgacca
     1861 tgcacgccta cggccccgac aaccagaccg tcgcctggac ccgaccgatc tgccgcttcc
     1921 cgcagcgcac ggtgctgaag gacggcgccg gcgactggca cgatccggcg agctggacct
     1981 gccgctgagg cggagcgggg ctcgcagagc cgagccccgt cggcaacgcg tgcgcatgga
     2041 tgcgcaccct acccggctga gtgcagagaa gcccgaagaa ctttttcttc tggcgtgagt
     2101 ccacatcttc agagtggcta tactcactct taatgtagct ctgta
//
LOCUS       DAOQJH010000542         2133 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_40671_length_2133_cov_4.534649, whole genome shotgun sequence.
ACCESSION   DAOQJH010000542 DAOQJH010000000
VERSION     DAOQJH010000542.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40671_length_2133_cov_4.534649"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="PLQ18_11455"
     CDS             complement(<1..233)
                     /locus_tag="PLQ18_11455"
                     /inference="COORDINATES: protein motif:HMM:NF025691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4389 domain-containing protein"
                     /protein_id="HNE02311.1"
                     /translation="MDERLKTNLRRQSFWQRAAYMLVFAVILGFAGSLAALLALFQLG
                     ALLLAGEPNARLRPVADGLTRYLLQLWRYQTFLT"
     gene            complement(577..1140)
                     /locus_tag="PLQ18_11460"
     CDS             complement(577..1140)
                     /locus_tag="PLQ18_11460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02312.1"
                     /translation="MRNAHSALYSAVLAGILAIGIGSAAQAAMSEAQYDAAVKAADAR
                     LKTDKERCSTMSGNAEDVCKAQAEAEHTRVTEQAKAEMKGTSKARLEAAEEVAEANYK
                     VAKERCDDFSGDPKDICLKEAKATYEKQKADITVLEKGNTADARKDARETKRDADFEV
                     AKAKCDQFSGDVKDKCVKDAKATYKVE"
     gene            complement(1287..1484)
                     /locus_tag="PLQ18_11465"
     CDS             complement(1287..1484)
                     /locus_tag="PLQ18_11465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006397770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HNE02313.1"
                     /translation="MNEDIIKGKWKQLKGSLKAKWGDLTDDDLDIAEGNSDYLAGRIQ
                     ERYGRTKDEAKREVDEYTRTL"
     gene            1774..>2133
                     /locus_tag="PLQ18_11470"
     CDS             1774..>2133
                     /locus_tag="PLQ18_11470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003283407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HNE02314.1"
                     /translation="MSLALAHCRAQLGIDAPPVAVEVHLSNGLPGLAIVGLPEAAVKE
                     SKDRVRGAILNSRHEYPQRRITVNLAPADLPKEGGRFDLPIALGILAASGQLPTEPLA
                     ALECLGELALGGALRGVR"
BASE COUNT          324 a          679 c          709 g          421 t
ORIGIN      
        1 gtcaggaagg tctggtagcg ccagagctgg agcaggtagc gcgtcaggcc gtcggcaacc
       61 gggcgcagcc gcgcgttcgg ctcgccggcc agcagcagcg cgccgagctg gaacagcgcg
      121 agcagcgccg cgaggctgcc ggcgaagccg aggatcaccg cgaacaccag catgtaggcg
      181 gcgcgctgcc agaagctctg gcggcgcagg ttggtcttca gtcgttcgtc catcgttcgg
      241 gtacctgggc aggtggacga ccgccggacg gcggccgggc gcgcggcacg acaggcgacg
      301 cgtcgctgca agcgtagatg gcggcgcccg cggcggattt catcgggcgg ttgcacgccg
      361 ttacaagaag agcgcgctgc ggcacgtggg cgtacccgtc ggtgtccgca gacgaatgtc
      421 atcggattgc cacgaatacc gctcaaaccg atacggacgc cacgctatcc agccgcagcc
      481 ggcgcgtggc gtccgtatcg gtgcaactcg gtacagatca gtacgatcag tacggatcgg
      541 tccggcgggg aaatccggat cgcgatgcgg tctgattcat tcgaccttgt aggtcgcctt
      601 cgcatccttc acgcacttgt ccttgacgtc gccgctgaac tggtcgcact tggccttggc
      661 gacttcgaaa tcggcgtcac gcttggtttc cctggcatcc ttgcgggcat cggccgtatt
      721 gcccttttcg agcacggtga tgtcggcctt ctgcttttcg taggtggcct tggcttcctt
      781 caggcagatg tccttcggat cgccggagaa atcatcacag cgttccttgg caaccttgta
      841 attggcttcg gcgacttctt cggcggcttc gagacgggcc ttgctggtgc ccttcatttc
      901 cgccttggcc tgttcggtga cgcgcgtgtg ttcggcttcg gcctgggcct tgcagacatc
      961 ctcggcattg ccggacatcg tcgaacacct ttccttgtcg gtcttgaggc gcgcatcggc
     1021 ggccttgacg gcggcatcgt actgggcctc gctcatcgcc gcctgtgcgg cggaaccgat
     1081 gccgatggcc aggatgccgg ccagaacggc gctgtacagg gcactgtgag cattacgcat
     1141 gatgttccac tcccgggggg ccgggcgcac cggtggcacg atgcgtacca ccggttgcgc
     1201 atccggtttt ggtatgggca gagaatcaag ggcccgcatc aggacgggcg ggtgccgttt
     1261 ccggcaccgg gcgaatcacg aaggaattac agcgtgcgcg tgtattcgtc gacttcgcgc
     1321 ttggcttcat ccttggtgcg gccgtaacgt tcctgaatgc ggccggcgag gtagtcgctg
     1381 ttaccttcgg cgatgtcgag atcgtcatcg gtcagatcgc cccacttggc cttgagcgaa
     1441 cccttgagct gcttccactt gcccttgatg atgtcttcat tcatggtcgt ttcctcctga
     1501 gggcaaatcc gttcccgctc ggcgaaatgc cgacggtcga acctgaattt gccggtttgg
     1561 ggatccgtcg ggcgggaaag cattcccgtc gtgtcccgac ggcggtaaag accggggcat
     1621 ttcgcgggta gttccgcagg cgtcattttt ttcgatcgaa aagcacagat tccctgatta
     1681 aaaatcgttc atgtcggggg ctgcggccgt gtggccacgg ctggctatgc tgccggaatc
     1741 agggatttcg ccgccgcacg gaggccccgc cgcatgtcgc tcgctctcgc ccactgccgc
     1801 gcccagctcg gcatcgacgc gccgccggtc gcggtcgagg tccacctgtc gaacggcctg
     1861 ccggggctgg cgatcgtcgg cctgccggag gcggccgtga aggagagcaa ggaccgcgtg
     1921 cgcggcgcga tcctgaactc gcgccacgaa tatccgcagc ggcgcatcac cgtgaatctg
     1981 gccccggccg acctgccgaa ggaaggcggg cgcttcgacc tgccgatcgc gctcggcatc
     2041 ctcgccgcca gcggtcagct gccgaccgag ccgctcgccg ccctcgaatg cctcggcgaa
     2101 ctcgcgctcg gcggcgcgct gcgcggcgtg cgt
//
LOCUS       DAOQJH010000543         2129 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_40797_length_2129_cov_4.573770, whole genome shotgun sequence.
ACCESSION   DAOQJH010000543 DAOQJH010000000
VERSION     DAOQJH010000543.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2129
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40797_length_2129_cov_4.573770"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1722)
                     /locus_tag="PLQ18_11475"
     CDS             complement(<1..1722)
                     /locus_tag="PLQ18_11475"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HNE02315.1"
                     /translation="MLQTIRDRASGWFAYLVFGMLLIPFALWGINYYTHDGTAPFVAR
                     VNGEEIGLARYQQTLQNQRERLRQGGVQEVDENRLKHMIVGQLIDERLLEQFTRAEHM
                     RAGNVQVSAWVQSVEAFRAGDRFDPETYQAVLRRQGYTVTDFENMLRTSLATDQLRQG
                     LAATALLSTTDRSRLIELVAQKRDFEVLTLALAPVSARQSVDDAAIEARFRAQPQAYV
                     FPEQLRLEVLDLSPDLLLSRVGTPQEADLRGLYTQQQASLGRPETRTASHILVRVPDG
                     ASPGQVEQAHQQALGYRETVVSGKESFEALGKSLAGTANVEVGDLGALERGVMDPAFD
                     QALFGLGKVGDLSEPVRTDFGFHVIRLDAVTPGSVPPFEEVRAQLVQLWQRQQAESAF
                     FDAADRLSTLVFENPDNLSTAARELGLQTRTSDWLTRSGSDDPLLNDARVLAAAFSDE
                     VLKDGRNSEPVQLDAQHVVVVRVAEHRDAKPKTLAEAREQIVAELKDSQARAELAKAA
                     EQLMQAVRDGTAPEAAAQAAGATRQAPGPLTRNAAVLTPELLRAAFRVPPPAGSQPAL
                     SVLELASG"
     gene            complement(1897..1973)
                     /locus_tag="PLQ18_11480"
     tRNA            complement(1897..1973)
                     /locus_tag="PLQ18_11480"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1937..1939),aa:Asp,seq:gtc)
BASE COUNT          336 a          738 c          721 g          334 t
ORIGIN      
        1 gccgctggcc agctccagca cgctgagcgc gggctggctg ccagccggcg gcggcacccg
       61 gaaggctgcg cgcagcagtt ccggcgtcag caccgccgcg ttgcgggtca gcgggccggg
      121 agcctggcgc gtcgcaccgg ccgcctgcgc cgccgcctcc ggcgctgtgc catcgcgcac
      181 ggcctgcatc agctgctcgg ccgccttcgc cagttcggcg cgcgcctgac tgtccttcag
      241 ctctgcgacg atctgctcgc gcgcttcggc gagcgtcttc ggcttggcat cgcgatgctc
      301 ggcgacgcgc acgacgacga cgtgctgggc atcgagctgc accggctcgc tgttgcgccc
      361 gtccttcagc acctcgtccg agaacgccgc cgccagcacg cgcgcatcgt tgagcagcgg
      421 atcgtcgctg ccgctgcgcg tcagccagtc gctggtgcgc gtctgcaggc cgagttcgcg
      481 ggcagcggtg ctcaggttgt cggggttttc gaagaccagc gtcgagagcc ggtcggcagc
      541 atcgaagaat gcgctctcgg cctgctggcg ctgccagagc tgcacgagct gcgcccggac
      601 ctcctcgaac ggcggcacgc tgcccggcgt caccgcatcg agccggatca cgtggaagcc
      661 gaaatcggtg cgcaccggtt cactgagatc gccgaccttg ccgagaccga acagcgcctg
      721 atcgaaagcc gggtccatga cgccgcgctc gagcgcgccg agatcgccga cctcgacgtt
      781 ggccgtgccg gccagggact tgccgagcgc ctcgaagctt tccttgccgc tgacgacggt
      841 ctcgcggtag ccgagcgcct gctgatgcgc ctgctcgacc tgcccgggac tcgcgccgtc
      901 cggcacgcgc acgaggatgt gcgaggcggt gcgggtttcc ggccggccga ggctggcctg
      961 ctgctgcgtg tacagccccc tgagatcggc ctcctgcggc gtgccgaccc gcgacagcag
     1021 cagatccggg ctcagatcca gcacttcgag ccgcagctgc tcggggaaga cataggcctg
     1081 cggctgcgcg cggaaacggg cctcgatggc cgcgtcatcg acgctctgac gcgcgctgac
     1141 cggcgccagc gccagcgtca gcacctcgaa atcgcgcttc tgcgcgacca gttcgatcag
     1201 ccggctgcgg tcggtcgtgc tgagcagcgc ggtcgctgcc agcccctgac gcagctgatc
     1261 ggtggccagc gaggtgcgca gcatgttctc gaagtccgtg accgtataac cctggcggcg
     1321 cagcacggcc tgataggttt cgggatcgaa gcgatcaccg gcccggaagg cctcgaccga
     1381 ctgcacccag gccgagacct gcacgttgcc ggcccgcatg tgctcggcac gggtgaactg
     1441 ctccagcaga cgctcgtcga tcagttgccc gacgatcatg tgcttgagcc ggttctcgtc
     1501 gacctcctgc acgccgccct gacgcagccg ctcgcgctgg ttctgcaggg tctgctggta
     1561 acgcgccagc ccgatctctt cgccgttgac cctggcgacg aacggcgcgg ttccgtcatg
     1621 ggtgtaatag ttgatgcccc agagcgcgaa cggaatgagc agcattccga acacgaggta
     1681 agcgaaccag cctgaggcac ggtcccggat cgtctgcagc atgaacagga tttccgtgac
     1741 ggagagacgg aacacggatg cgccggctcc atgcagttac cctgttcgcc ggcatcatcc
     1801 gcaacggatg tggctccagt ctaacgcaat ggccagccac agggcgcacg atcggcgccg
     1861 gtgaggccgc gcactggaaa cgggaaaaac agggaatggc ggagcggacg gggctcgaac
     1921 ccgcgacccc cggcgtgaca ggccggtatt ctgaccaact gaactaccgc tccggaattt
     1981 gcagatcggc attgcgccga atcatcgccg tgccggagtc gcccggacga atccggtccg
     2041 cgacttccgc agggacgatg aatttcagga atgattggcg gagcggacgg ggctcgaacc
     2101 cgcgaccccc ggcgtgacag gccggtatt
//
LOCUS       DAOQJH010000544         2124 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_40987_length_2124_cov_3.260029, whole genome shotgun sequence.
ACCESSION   DAOQJH010000544 DAOQJH010000000
VERSION     DAOQJH010000544.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2124
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40987_length_2124_cov_3.260029"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..915
                     /gene="rlmN"
                     /locus_tag="PLQ18_11485"
     CDS             <1..915
                     /gene="rlmN"
                     /locus_tag="PLQ18_11485"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002810450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HNE02316.1"
                     /translation="ASGDGSHKWLIRVDSGNCIESVFIPETDRGTLCVSSQIGCALDC
                     SFCSTAQQGFNRNLSVAEIVGQVWAANHALGWGSNDTRIITNVVMMGMGEPLANFANV
                     VAAMQIMLDDFGFGLSKRRVTLSTSGIVPALYRLAEVTDVSLAVSLHAPDDALRDELV
                     PINRKYPIAELLAACAHYISDKPHRRVTWEYVMLDGVNDSDAHAHALAQLIRHIPSKI
                     NLIPFNPFPGTRYRRSPQARIDRFRDILIGGYGITTVTRRTRGDDIDAACGQLAGRVQ
                     DRSRRVTQVVAIEPAVRGAGTAFGQPSA"
     gene            912..1730
                     /locus_tag="PLQ18_11490"
     CDS             912..1730
                     /locus_tag="PLQ18_11490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNE02317.1"
                     /translation="MRTGLRIVCVLGAVAMLGGCASWFGDGGDKEKNLATTTNVRAPS
                     TDPFEIDKKQAAQLNLQLGVGYIRQGDYQVAVSKLRKSLSFDDDSAEAHNALGIALEE
                     LRSYDEAAREYDRALALKSGYTAALINRTRLQCVRGTQAAEGEQRFIELAGSGTLEAP
                     ETAWIEAASCARRQQAWARAGAHLDRALEVAPGAMAQVLYERALLAFEQRNLPQAREL
                     LRRLHGDYGSSARSLGLAVRVEDALGGSAQRRQLVAELLRAYPDSPEARSYRPR"
     gene            1727..>2124
                     /locus_tag="PLQ18_11495"
     CDS             1727..>2124
                     /locus_tag="PLQ18_11495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012510798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNE02318.1"
                     /translation="MNAPEPVLPVSGPTVGRRLAAAREQRGLDQAAAARLSGLSAEVV
                     RGLEAEDWAALGAPVYVRGWLARYCRVLDVEASSALAEYRQAVPAEPPPLRLRGEAPS
                     SAPRGHHSLRPFAYLLSFALLGYVGWQAFE"
BASE COUNT          308 a          767 c          736 g          313 t
ORIGIN      
        1 gcctcgggcg acggttcgca caagtggctg atccgcgtcg acagcggcaa ctgcatcgaa
       61 tcggtgttca tcccggagac cgaccgcggc acgctgtgcg tgtcctcgca gatcggctgc
      121 gcgctcgact gcagcttctg ctcgaccgcg cagcagggct tcaaccgcaa cctgtcggtc
      181 gccgagatcg tcggtcaggt ctgggccgcc aaccacgcgc tcggctgggg cagcaacgac
      241 acgcgcatca tcaccaacgt cgtgatgatg ggcatgggcg agccgctcgc gaacttcgcg
      301 aacgtggtcg cggcgatgca gatcatgctc gatgacttcg gcttcggcct gtcgaagcgg
      361 cgcgtgacgc tgtcgacctc gggcatcgtg ccggcgctgt accggctcgc cgaggtcacc
      421 gacgtgagcc tcgccgtgtc gctgcacgcg cccgacgatg cgctgcgcga tgaactcgtg
      481 ccgatcaacc gcaagtaccc gatcgccgag ctgctcgcgg cctgcgcgca ctacatcagc
      541 gacaagccgc accggcgcgt gacctgggag tacgtgatgc tcgacggcgt caacgactcc
      601 gatgcgcacg cgcacgcgct cgcgcagctg atccggcaca tcccgtcgaa gatcaacctg
      661 atcccgttca acccgttccc gggcacgcgc taccggcgct cgccgcaggc gcgcatcgac
      721 cgcttccgcg acatcctgat cggcggctac ggcatcacca ccgtcacgcg ccgcacgcgc
      781 ggcgacgaca tcgacgccgc ctgcggccag ctcgccgggc gcgtgcagga tcgcagccgc
      841 cgcgtcacgc aggtggtggc gatcgagccg gccgtgcgtg gggcggggac tgcgttcgga
      901 cagccgtcgg catgaggacg ggcctgcgca tcgtctgcgt gctgggcgcc gtcgccatgc
      961 tcggtggctg cgcctcgtgg ttcggcgacg gtggtgacaa ggaaaagaac ctcgccacga
     1021 ccacgaacgt ccgggcgccg agcaccgacc ccttcgagat cgacaagaag caggccgcgc
     1081 agctgaacct gcagctcggt gtcggctaca tccgtcaggg ggactaccag gtcgccgtga
     1141 gcaagctgcg caagtcgctg agtttcgacg acgattcggc cgaggcgcac aatgcgctcg
     1201 gcatcgcgct cgaagagctg cgcagctatg acgaagccgc ccgcgagtac gaccgggcgc
     1261 tggcgctgaa atccggctat accgcggcgc tgatcaaccg cacgcgcctg cagtgcgtgc
     1321 gcggcacgca ggcggccgaa ggcgagcagc gcttcatcga gctggccggt tccggcaccc
     1381 tcgaagctcc ggaaaccgcc tggatcgagg ccgccagctg tgcccggcgc cagcaggcct
     1441 gggcgcgggc cggcgcgcat ctcgatcgcg cgctcgaggt ggcaccgggc gcgatggccc
     1501 aggtgctgta cgagcgtgcg ctgctcgcct tcgagcagcg caacctgccg caggcccgcg
     1561 agctgctgcg ccggctgcac ggcgattacg gcagcagcgc gcgcagcctc ggcctcgccg
     1621 tgcgcgtcga ggacgcgctc ggcggcagtg cgcagcgtcg ccagctcgtc gccgagctgc
     1681 tgcgtgccta tcccgattcg cccgaagccc ggtcttaccg tccgcgatga acgcacccga
     1741 gcccgtcctt cccgtatccg gaccgaccgt cggccggcgt cttgctgcgg cccgcgagca
     1801 gcgcggcctg gatcaggccg ctgcggcgcg cctcagcggg ctgagtgccg aggtcgtgcg
     1861 cggcctggag gccgaggact gggccgcgct cggggcgccg gtctacgtgc gcggctggct
     1921 ggcccgctac tgccgcgtgc tcgatgtcga agcctcgtcg gcgctcgccg aataccgtca
     1981 ggcggtgccg gccgagccgc cgccgctgcg cctgcgtggc gaggccccga gttccgcccc
     2041 gcgcggccat cattcgctgc ggccgtttgc atacctgctg agcttcgcgc tgctcggcta
     2101 cgtcggctgg caggccttcg agaa
//
LOCUS       DAOQJH010000545         2120 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_41118_length_2120_cov_5.170944, whole genome shotgun sequence.
ACCESSION   DAOQJH010000545 DAOQJH010000000
VERSION     DAOQJH010000545.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2120)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2120)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2120
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41118_length_2120_cov_5.170944"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..733)
                     /gene="mnmA"
                     /locus_tag="PLQ18_11500"
     CDS             complement(<1..733)
                     /gene="mnmA"
                     /locus_tag="PLQ18_11500"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012734672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HNE02319.1"
                     /translation="MKPQRIVVGLSGGVDSAVSAWLLREQGHEVHAVFMKNWDDALDT
                     GWCSAAEDLRDCEDICATLDIPLHRVNFAREYRERVFAAFLSELAAGRTPNPDVLCNR
                     EIKFRAFLDHALALGADALATGHYARTARTADGRVQLLKGLDPGKDQSYFLHALEQRQ
                     LAPACFPVGALPKREVRAIAARLGLVVHDKKDSTGICFIGERDFTQFLAQYLPAEPGP
                     MRTPDGECVGTHRGLAFYTLGQRQGL"
     gene            1078..>2120
                     /locus_tag="PLQ18_11505"
     CDS             1078..>2120
                     /locus_tag="PLQ18_11505"
                     /inference="COORDINATES: protein motif:HMM:NF026177.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HNE02320.1"
                     /translation="MEPASPVPGQGHFISLKWRALLLTSIVLVVMIFGGAWLNYDNLS
                     RQLRTQLAQDNAARLRELHALMGQSVERLRQLAGMIPTFPGMQTALRGNDGLAVASVL
                     DAQWPALQFDLGLDRLVVYGSDDASALAQWSVSSGTEVEARLVGLVAMARAQETPQSL
                     LECSPECMHYVAAPMLAEGRNAGVVLLGVGLADTLRSFAAVSGAEVGLLRIGARGSRD
                     PALGRLDAWNADLSALTRPEQSLPVLRLAAGRQALEDAAGGLLVEAGERSYDLRVSTL
                     PGLARDHPIRLLVIADITAPLSDIRAAAQTAFGIGAIGWLLAECALLAILWRPMSRLR
                     KTAQTLPLLAQSE"
BASE COUNT          297 a          725 c          787 g          311 t
ORIGIN      
        1 ccaggccctg acgctggccg agcgtgtaga aggccaggcc gcggtgcgtg ccgacgcact
       61 cgccgtcggg cgtgcgcatc gggccgggct cggccggcag gtactgcgcg aggaactgtg
      121 tgaagtcgcg ctcgccgatg aagcagatgc cggtcgaatc cttcttgtcg tgcacgacca
      181 ggccgaggcg cgcggcgatg gcgcgcacct cgcgcttggg cagcgcgccg accggaaagc
      241 aggccggggc gagctggcgc tgttcgagcg cgtgcaggaa gtagctctgg tccttgcccg
      301 gatcgaggcc cttcagcagc tgcacgcgac catcggcggt gcgggccgtg cgcgcgtagt
      361 ggccggtggc gagcgcatcg gcaccgaggg ccagcgcatg gtcgaggaag gcgcggaact
      421 tgatctcgcg gttgcagagc acgtcggggt tcggcgtgcg tccggcggcg agttcggaca
      481 ggaaggccgc gaacacgcgc tcgcggtatt cgcgcgcgaa gttcacgcgg tgcagcggga
      541 tgtcgagcgt cgcgcagatg tcctcgcagt cgcgcagatc ctcggcggcg ctgcaccagc
      601 cggtgtcgag cgcatcgtcc cagttcttca tgaacacggc gtggacctcg tggccctgct
      661 cgcgcagcag ccacgcgctg acggcggaat cgacgccgcc ggacaggccg acgacgatgc
      721 gctggggttt catgcgaagg aaaatccggt tcgggcggaa aggcggccgg cgggccgggg
      781 agggatgcag cagccatgat accggcacgc cgtgcggatg ccgtactgaa gcccggcgcc
      841 gcgcagccga tgcccgggcg atgggctggg cgctgccgtt tccgccggcc ggcggcttgc
      901 cgggtcaccg gcgtccgggg cagcctgtgc agtctgcaag gcatgctgca ctgcgacatg
      961 cgatttgacg gttgcaaggc caccggatgg cataccctgt ccacaccggt ccgaccacac
     1021 ggacatcgcc ttttcagcag tcgggcatcg gcccaccggc gcatggcatt gcagaatatg
     1081 gaacccgcat cgcccgtccc cggccagggt cacttcatca gcctcaaatg gcgcgcgctg
     1141 ctgctgacca gcatcgtgct ggtcgtgatg atcttcggcg gggcctggct gaactacgac
     1201 aacctgagca ggcagctgcg cacgcagctc gcgcaggaca acgccgcacg cctgcgcgaa
     1261 ctgcatgcgc tgatgggcca gtcggtcgag cgcctgcgtc agctcgcggg catgatcccg
     1321 acctttccgg gcatgcagac ggcgctgcgc ggcaacgacg ggctggcggt cgccagcgtg
     1381 ctcgatgcgc agtggccggc cctgcagttc gatctcggcc tcgaccggct cgtggtctac
     1441 ggcagcgacg acgccagcgc gctcgcgcag tggtcggtga gttcgggcac cgaggtcgag
     1501 gcgcggctgg tcgggctggt ggcgatggcg cgcgcgcagg agacgccgca gagcctgctc
     1561 gaatgcagcc ccgagtgcat gcattacgtc gccgcaccga tgctcgccga gggccgcaac
     1621 gccggcgtcg tgctgctcgg cgtcggactg gccgacacgc tgcgcagctt cgccgccgtc
     1681 tccggcgccg aggtcgggct gctgcgcatc ggcgcccgcg gcagccgcga tccggcgctc
     1741 ggccggctcg atgcctggaa cgccgacctc tcggcgctga cccgtcccga gcagtcgctg
     1801 ccggtgctga ggctcgcggc cggccggcag gcgctcgagg atgccgccgg cggcctgctg
     1861 gtcgaggccg gcgagcgcag ctacgacctg cgggtgtcga cgctgccggg cctggcccgc
     1921 gaccatccga tccggctgct ggtgatcgcc gacatcacgg cgccgctctc cgacatccgc
     1981 gccgccgcgc agacggcctt cggcatcggc gcgatcggct ggctgctcgc cgaatgcgcg
     2041 ctgctcgcga tcctgtggcg gccgatgtcg cggctgcgca agacggcgca gacgctgccg
     2101 ctgctcgcgc agagcgagtt
//
LOCUS       DAOQJH010000546         2117 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_41226_length_2117_cov_4.449079, whole genome shotgun sequence.
ACCESSION   DAOQJH010000546 DAOQJH010000000
VERSION     DAOQJH010000546.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2117
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41226_length_2117_cov_4.449079"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..269
                     /locus_tag="PLQ18_11510"
     CDS             <1..269
                     /locus_tag="PLQ18_11510"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HNE02321.1"
                     /translation="PAEAKNVRCPVLACHGADDPLVTQDVVAGFEREMSDAGVDWTVV
                     QFGGVMHSFTNPQANDPGFGTVYDAGADRRAFGLLRQYLDDVFA"
     gene            266..457
                     /locus_tag="PLQ18_11515"
     CDS             266..457
                     /locus_tag="PLQ18_11515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02322.1"
                     /translation="MNPKPPAPSPAPAPARGPDDELADLDDAELDARCCQNFCKYCVK
                     VRAARARARRAGLPLPPWQ"
     gene            606..1334
                     /locus_tag="PLQ18_11520"
     CDS             606..1334
                     /locus_tag="PLQ18_11520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrdJb"
                     /protein_id="HNE02323.1"
                     /translation="MTVKIDSKIVKYAVLSKEETTPAPVAVVPVAEPEPVQQALGFSL
                     EQMHEALERPEMLLGSTYKVKTPLSEHAMYITINDIVLNPGTPHEQRRPFEVFINSKN
                     MDHFQWIVALTRIISAVFRKGGDVTFLVEELKSVFDPKGGYFKRGGKYMPSLVAEIGD
                     VIESHLRLIGLLKDDGLDEHQQKLVAEKRAQYAARSVSDVAGGTAAGEFPVGATLCNK
                     CQTRAVIVMDGCMTCLNCGDSKCG"
     gene            1455..2117
                     /locus_tag="PLQ18_11525"
     CDS             1455..2117
                     /locus_tag="PLQ18_11525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02324.1"
                     /translation="MPRRRTLDLAGTIGCIDTRGLKTTLISTVGTHCDVWRTTRMVSR
                     EGGALMLDLVIKRPKLAVSHAEIRIMRRDWKILHDRLGDIVPEAIFVETEVQGSPNVV
                     VVAEAAPLWFNVANPAYEEDAVMLMRQNPKSRAQLGLFIEAARDWYDQGNGKLIDLFG
                     LDNLVIDVNRRVRYVDSFNVFFYLDLLDMEDDADSHLRNMVEISLERLDYLDWVMRLT
                     RP"
BASE COUNT          362 a          739 c          694 g          322 t
ORIGIN      
        1 atccggccga ggcgaagaac gtccgctgcc cggtgctcgc ctgccacggc gccgacgatc
       61 cgctggtcac gcaggacgtg gtcgccggtt tcgagcgcga gatgagcgat gccggcgtcg
      121 actggaccgt cgtgcagttc ggcggcgtca tgcactcgtt cacgaacccg caggccaacg
      181 atccgggctt cggcaccgtc tacgacgccg gcgccgaccg ccgcgccttc gggctgctgc
      241 gtcagtacct cgacgacgtg ttcgcgtgaa cccgaagcca cccgcgccgt cgccggcccc
      301 ggcgcctgcc cgggggccgg acgacgaact ggccgatctc gacgacgccg aactcgacgc
      361 gcgctgctgc cagaacttct gcaagtactg cgtgaaggtc cgcgccgcgc gcgcccgtgc
      421 ccggcgcgcc ggcctgccgc tgccgccgtg gcaatgagcc gggccgctgg ccagccgcgc
      481 ggcgggcagg cacaatgcgc gccccggtca tgcacaacat gctgtgtgca gatcagtaca
      541 aaaacacatc atctggttat cggcgccttg ctggcgccgg ccgacccgca gcccgagtgc
      601 cgaccatgac cgtcaagatc gacagcaaga tcgtcaagta cgccgtgctg agcaaggaag
      661 aaacgacgcc ggcgccggtg gccgtcgtgc cggtcgcgga gcccgagccg gtgcagcagg
      721 cgctcggctt cagcctcgaa cagatgcacg aggcgctgga gcgcccggag atgctgctcg
      781 gctcgaccta caaggtgaag acgccgctgt ccgagcacgc gatgtacatc acgatcaacg
      841 acatcgtgct gaacccgggc acgccgcacg agcagcgccg gccgttcgag gtgttcatca
      901 actccaagaa catggaccac ttccagtgga tcgtggcgct gacgcgcatc atctcggcgg
      961 tgttccgcaa gggcggcgac gtgaccttcc tggtcgagga gctgaaatcg gtcttcgatc
     1021 cgaagggcgg ctacttcaag cgcggcggca agtacatgcc gtcactggtc gccgagatcg
     1081 gcgatgtcat cgaatcgcac ctgcgcctga tcggcctgct gaaggacgac ggcctcgacg
     1141 agcaccagca gaagctggtc gccgagaagc gcgcgcagta cgcggcgcgc agtgtcagcg
     1201 atgtcgccgg cggcacggcg gccggcgagt ttccggtcgg cgcgacgctc tgcaacaagt
     1261 gccagacccg cgccgtcatc gtcatggacg gctgcatgac ctgcctcaac tgcggcgatt
     1321 cgaagtgcgg ctgaacgcgc ctgcgccgac tggcaggcga cagcccggtg cgctcatgat
     1381 tcactgctga accttcaccg ctgaaccggc cgtcgcgccg ccccctgtcc gccgagcccg
     1441 aggaccgctc cgccatgccg agacgccgca cgctggacct tgccggcacc atcggctgca
     1501 tcgacacgcg cggtctgaag accacgctga tctccaccgt cggcacgcac tgcgatgtct
     1561 ggcgcacgac gcgcatggtc tcgcgcgagg gcggcgcgct gatgctcgat ctcgtcatca
     1621 agcgccccaa gctcgccgtc tcgcacgccg agatccgcat catgcggcgc gactggaaga
     1681 tcctgcacga ccggctcggc gacatcgtgc cggaggcgat cttcgtcgag accgaggtcc
     1741 agggctcgcc gaacgtcgtc gtcgtcgccg aggccgcgcc gctgtggttc aacgtcgcca
     1801 atccggccta cgaggaggat gcggtgatgc tgatgcgcca gaacccgaag tcgcgcgcgc
     1861 agctcggcct gttcatcgag gcggcgcgcg actggtacga ccagggcaac ggcaagctga
     1921 tcgacctgtt cggcctcgac aacctggtca tcgacgtgaa ccgccgcgtg cgctacgtcg
     1981 acagcttcaa cgtcttcttc tatctcgacc tgctcgacat ggaggacgac gccgactcgc
     2041 acctgcgcaa catggtcgag atctcgctgg agcggctcga ttatctcgac tgggtgatgc
     2101 ggctgacgcg gccgtga
//
LOCUS       DAOQJH010000547         2113 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_41386_length_2113_cov_3.019922, whole genome shotgun sequence.
ACCESSION   DAOQJH010000547 DAOQJH010000000
VERSION     DAOQJH010000547.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41386_length_2113_cov_3.019922"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /locus_tag="PLQ18_11530"
     CDS             <1..238
                     /locus_tag="PLQ18_11530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008995034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HNE02325.1"
                     /translation="HYCVANMPGAVPRTSALALNNATLPYLLALADRGWVRALGNDPG
                     LLNGLNVLRGRVTHRAVAEALGHDYHPAGDLLAL"
     gene            518..>2113
                     /locus_tag="PLQ18_11535"
     CDS             518..>2113
                     /locus_tag="PLQ18_11535"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF017081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNE02326.1"
                     /translation="MPLTDYVPDFRSPIPRGRIASTLMLLAFYLLAAGIGLRLQVYYG
                     AVTPLWPPSGLAVWMLWRRGLGLAPVILGGEWLVALWLGQGWQAQILGPLAQLVEAML
                     ASMLLRLARLPARPLDTLHGLQLFLLDAVLLAPMLAAAIGTWGLYLTGATGDGPLYET
                     LLTWWTGDALGILIITPLLLAWWPQPARSTVAQAAALMIATGIAALVVLMQAPEQRTM
                     LFFLLLPFVAIAGLLRDPAVVTLTILMLVVLALGLQAIAIGHPDEFVKHVRLLFIGTV
                     AATGYIVSVLAQRLHRLANTDPLTGLPNRRRFTEQLEALAARPRGRRHALLYLDLEAF
                     KLANDTCGHAAGDRLLVEVTGRLLRRKPAAATLARMGGDEFVLLAPDHDADEASALAH
                     RLREALASKPFTVGERSFGIGMRIGIAPFGPRSESPEGVLARADLACAAARLHGGVRV
                     SGPGDGALLRHQGEIALLERLRAAFGNGRLRLAWQRLDPLPGREDGRARREMLLRLLG
                     DDGHPLGPDTFLPVAERYGLLPQI"
BASE COUNT          270 a          789 c          725 g          329 t
ORIGIN      
        1 ccactactgc gtcgccaaca tgcccggtgc cgtgccgcgc acctcggcgc tggcgctgaa
       61 caacgccacg ctgccgtatc tgctcgcgct cgccgaccgc ggctgggtcc gcgcgctcgg
      121 caacgacccg ggtctgctga acggcctgaa cgtcctgcgc ggccgcgtca cgcaccgcgc
      181 cgtcgccgag gcgctcggcc acgactacca cccggccggc gatctgctgg cgctgtgatc
      241 cgctgccgcc agcggcatgc cggcgacgac tccggcacgg cacggccatg gacggcggca
      301 gggcagccgt ccgctcccgg aaatcccggc aggcgcaagc gcttccggca cccggaaatc
      361 cgggaatgaa acggttgttt caagccaaaa tcatgcagta aaaataaggc aataaggctc
      421 ctcacagcgg gcggcggctc cggtcccggc agcggtcgct ccgtccggca gtccctccac
      481 gctcctgcct tgccctgccc tgcggaagga tgccgacatg ccgctgaccg attacgtgcc
      541 cgatttccga tcaccgatcc cgcgtggccg gatcgccagc acgctgatgc tgctggcgtt
      601 ctatctgctc gccgccggaa tcggcctgcg cctgcaggtg tactacggtg ccgtcacgcc
      661 gctgtggccg ccgtccgggc tggccgtctg gatgctgtgg cggcgcgggc tcgggctggc
      721 accggtgatc ctcggcggcg aatggctggt ggcgctgtgg ctcggtcagg gctggcaggc
      781 gcagatcctc gggccgctcg cgcagctcgt cgaagccatg ctggcctcga tgctgctgcg
      841 gctggcgcgg ctgccggccc ggccgctcga cacgctgcac gggctgcagc tgttcctgct
      901 cgatgccgtg ctgctggcgc cgatgctggc agccgccatc ggcacctggg ggctgtatct
      961 gaccggtgcg accggcgacg ggccgctgta cgagaccctc ctgacctggt ggaccggcga
     1021 tgcgctcggc attttgatca tcacgccgct gctgctcgcc tggtggccgc agccggcgcg
     1081 cagcaccgtc gcgcaggccg cggcgctgat gatcgccacc ggcatcgcgg cgctggtggt
     1141 gctgatgcag gcccccgaac agcgcacgat gctgttcttc ctgctgctgc ccttcgtcgc
     1201 gatcgccggg ctgctgcgcg atccggcggt cgtgacgctg acgatcctga tgctggtggt
     1261 gctcgccctc ggcctgcagg ccatcgccat cggacacccc gatgaattcg tcaagcacgt
     1321 gcgcctgctg ttcatcggca ccgtcgctgc cactggctac atcgtctcgg tgctcgcgca
     1381 gcgcctgcac cggctggcga acaccgatcc gctgaccgga ctgccgaacc ggcgccgctt
     1441 caccgaacag ctcgaagcac tcgccgcccg cccccgcggc cggcgccacg cgctgctcta
     1501 tctcgacctc gaagccttca agctcgccaa tgacacctgc ggccacgcgg ccggtgaccg
     1561 cctgctggtc gaagtcaccg ggcgactgct gcggcgcaag ccggcggcag cgacgctggc
     1621 gcgcatgggc ggggacgagt tcgtgctgct cgcgccggat cacgacgccg acgaggcgag
     1681 cgcactggcg caccggctgc gcgaggcgct ggcctcgaaa ccgttcacgg tcggcgagcg
     1741 cagcttcggc atcggcatgc gcatcggcat cgcccccttc ggtccgcgca gcgaaagccc
     1801 ggagggcgtg ctggcgcgtg ccgatctggc ctgcgcagcg gcgcgcctgc acggcggcgt
     1861 gcgcgtctcc ggccccggtg acggcgcgct gctgcgtcat cagggcgaga tcgcactgct
     1921 cgaaaggctg cgcgccgcgt tcggcaacgg ccggctgcgg ctcgcctggc agcgcctgga
     1981 cccgctgccc ggtcgcgagg acggccgcgc gcggcgcgag atgctgctgc gcctgctcgg
     2041 tgacgacgga cacccgctcg gccccgacac cttcctgccg gttgccgagc gctacggcct
     2101 gctgccgcag atc
//
LOCUS       DAOQJH010000548         2110 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_41475_length_2110_cov_3.688564, whole genome shotgun sequence.
ACCESSION   DAOQJH010000548 DAOQJH010000000
VERSION     DAOQJH010000548.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2110
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41475_length_2110_cov_3.688564"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(223..>2110)
                     /locus_tag="PLQ18_11540"
     CDS             complement(223..>2110)
                     /locus_tag="PLQ18_11540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02327.1"
                     /translation="QPWPHGNLSRLLARELDCAEPALCSGPWAWNVLLGRAGQRELQP
                     DAQAPGGWRLLQGPAHLGGALADALRRGLDAVLASTPAALQDAALILIEGPLNDAALA
                     TDGAAPLAFGHYVQKRLTSALGSSTSRRIVTAGLRDALVARGCSVSATRLGAREIAYY
                     DFLPMLEINVLRAGEHAFAELIEPAERVAGGSSYTHTLADRFIVAKDTRSLVFYLLKE
                     DEAGARRSETLLPVPPSTTVEISLQVTQTPAQGYARVEIRPTKSGILGQSAILLDWTT
                     MTAITGSREDVLLELELQGLGHPDIAPQRTHRLLWKYKRGDGLTILKALTEFTASPLK
                     TTAHSQYKKLLVQTRSLAGLRTSPYLLTQGANPDRTACTAFDSDGNIPEEFALQMQPC
                     IEQFCNCLNSDFSAVKHARTRQDIQTRRNLSRLGATLYAACPDEIVHYYRLIARGTVS
                     DPTLVLHAGKVLCTEADLDSLFRYCADCSISAVKDGKKLSNFVLRAAGDALAYRETAG
                     VVLNAISADRLADAALMVLEEEINAINYKVRFRAAARLALFILRHRMRRRDFLHPSSR
                     DPANRRRAQRLEALITRSLASRHAGDDLTVALQELQAQIRYRGTNAIIDIDADAGDET
                     EDED"
BASE COUNT          344 a          632 c          741 g          393 t
ORIGIN      
        1 gcccacggat tccggccgcg cagccgtcgc gggctgaccg gcaacgcggc tggccgccat
       61 gaacgaggcg acccgaaggc cgccccgtgc ggtcagtgag cgtgccggct gccgctcccc
      121 gttcggatcg ttcggcccgc cgccgccctc gggcacgatt ccatcgatgt tccgggtcgg
      181 gtgctggatg tcgcaggcaa gtgcgggcgg ggcaggactg cttcagtcct cgtcctccgt
      241 ttcatcgcca gcgtctgcgt cgatgtcgat gatcgcgttg gtgccgcggt agcggatttg
      301 tgcctgcagt tcctgcaggg cgaccgtcag atcatcacca gcatgccggg atgcgagcga
      361 ccgggtaatc agagcttcga gacgttgggc ccgccggcgg ttggcagggt cgcgactcga
      421 ggggtgcagg aaatcgcggc ggcgcatcct gtggcgcagg atgaacagtg ccagccgggc
      481 agcggcacga aatctcacct tgtaattgat ggcgttgatt tcttcttcga gcaccatcaa
      541 tgcggcatcg gcaagcctgt ctgcggaaat ggcattgagg actacaccgg cggtttcccg
      601 gtaggccagc gcatcgccgg ctgcacgcag cacgaaattg gaaagctttt ttccgtcttt
      661 caccgcactg atcgagcagt cagcgcaata tcggaacagg ctgtcgaggt cggcttcggt
      721 gcagagcacc ttgccggcat gcagcaccag ggtcgggtca ctgacggtgc ctctggcgat
      781 cagccggtaa taatgcacga tttcgtcggg gcaggccgcg tagagcgttg ctccaagcct
      841 ggacaggttc cggcgtgtct ggatgtcctg tctggtccgg gcatgcttga cggcactgaa
      901 gtccgagttg aggcaattgc agaattgctc gatgcaaggc tgcatctgca aggcaaattc
      961 ttcagggatg tttccgtctg aatcaaaggc agtgcaggcg gtccgatccg ggttggcgcc
     1021 ctgtgtgaga agatatgggc tggtgcgcag accggcgagg ctgcgggttt gaacgaggag
     1081 ttttttgtac tgactgtgcg cagtggtttt cagggggctg gccgtgaatt cggtcaaggc
     1141 tttcagaatc gtcaggccgt caccgcgctt gtatttccag agcagccgat gcgtacgctg
     1201 cggtgcaatg tccggatgac ccagaccttg cagttccagt tcgagcagga catcttcccg
     1261 tgagccggtg atcgcggtca ttgtggtcca gtccagcaaa atcgcggact gcccgaggat
     1321 gccggatttc gtggggcgta tctcgacgcg cgcataaccc tgagccgggg tctgggtaac
     1381 ctgcagactg atttcgacgg ttgtggatgg cgggacgggc agcagggttt cgctgcggcg
     1441 cgctccggct tcgtcctctt tcagcaggta gaagaccagc gagcgcgtgt ctttggcgac
     1501 gatgaaacga tcggccagcg tatgcgtgta actgctgccc ccggcgacgc gctcggcagg
     1561 ctcgatcagt tcggcaaacg catgctcgcc ggcgcgcagc acgttgattt ccagcatcgg
     1621 caggaagtcg taataggcga tctcgcgggc gccgaggcgg gtcgcgctga cgctgcagcc
     1681 acgggcaacg agggcgtccc gaagtccggc ggtgacgatg cgccggcttg tggaactgcc
     1741 gagggccgaa gtgaggcgct tttgcacgta gtggccgaag gccagcggcg cagcgccgtc
     1801 cgtggcaagc gcggcatcgt tcagcgggcc ttcgatcagg atcagcgcgg cgtcctgcag
     1861 ggccgccgga gtgctcgcga ggactgcatc gaggccgcgg cgcagggcat cggccagcgc
     1921 gccgccgagg tgcgccggtc cctgcaggag gcgccagccg ccgggggcct gcgcatcggg
     1981 ctgcagctcg cgctgccccg cgcgtccgag caggacgttc caggcccagg ggccactgca
     2041 gagcgccggc tccgcgcagt ccagctcgcg cgccagcagc cggctcaggt tcccgtgcgg
     2101 ccaaggctgc
//
LOCUS       DAOQJH010000549         2102 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_41768_length_2102_cov_3.806546, whole genome shotgun sequence.
ACCESSION   DAOQJH010000549 DAOQJH010000000
VERSION     DAOQJH010000549.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2102)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41768_length_2102_cov_3.806546"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..781)
                     /locus_tag="PLQ18_11545"
     CDS             complement(<1..781)
                     /locus_tag="PLQ18_11545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008682334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNE02328.1"
                     /translation="MLQTLLICLAVGAFAGVMAGLLGIGGGLVIVPALAWVFHTQGVD
                     DGIVMHLALGTSLATIVFTSVSSVRAHHRRGAVLWEPVTRLAPGIVVGAWLGAQIADA
                     LPTATLKLVFAIFVLAVSAQIALGAQPAGQHRLPGRVGMALTGGLIGAVSGVVGIGGG
                     SLTVPFLTWCQVPIRNAVASSAAVGLPIAVSGALGYVAAGWNEPLLPSGALGYVSLTA
                     GAGIAAASMLTAPLGARLAHTLPTALLRRIFAGFLALVGLKM"
     gene            1009..1953
                     /locus_tag="PLQ18_11550"
     CDS             1009..1953
                     /locus_tag="PLQ18_11550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I NDUFA9 subunit family protein"
                     /protein_id="HNE02329.1"
                     /translation="MKVQTVCVLGGTGFVGRALVSRLANSGCKVRVLTRHPQRRCREI
                     EVVPNVSLLAIGDTPLAGHFKGCDAVINLVGILNEAGSQTFRGVHVELAKRVAAACVE
                     AGVPRLLHMSALNADAVNGVSDYLKTKGEGEDAVHATAGLKVTSFRPSVIFGCEDSFF
                     NRFAGLLKLAPVLPLACPEARFQPVYVRDVAAAFAQALEDERTVGKRLELGGPRIYTL
                     KELVEFTARTLGLSTLVIGLPDSVAKLQAKLMDKLPTRPFSLDNYRSLQQDSIVGGED
                     GFALLGIVPRSVEGVMPRELRARDHRGPYAAYRASAGR"
BASE COUNT          338 a          729 c          729 g          306 t
ORIGIN      
        1 gcatcttcag tccgaccagc gcgaggaagc ccgcgaagat gcgtctgagc agcgccgtcg
       61 gcagcgtatg cgcgagtctg gcaccgagcg gcgccgtcag catgctcgcc gcggcgatgc
      121 cggcgccggc cgtgaggctg acatagccga gcgcgccgga gggcagcagc ggctcgttcc
      181 agccggcggc gacgtagccg agcgcgccgg acaccgcgat cggcaggccg acggcggccg
      241 agctggcgac ggcgttgcgg atcggcacct ggcaccaggt caggaagggc acggtcagcg
      301 agccgccgcc gatgccgacg acgccggaca ccgcgccgat gaggccgccg gtgagcgcca
      361 tgccgacgcg ccccggcagc cggtgctggc cggccggctg cgcaccgagc gcgatctgcg
      421 ccgagacggc gagcacgaag atcgcgaaca ccagcttcag cgtcgcggtt ggcagcgcat
      481 cggcgatctg cgcgccgagc caggcgccga cgacgatgcc gggcgcgagc cgcgttaccg
      541 gctcccacag caccgcgccg cggcgatgat gcgcgcgcac cgaggacacc gacgtgaaca
      601 cgatggtcgc gagcgaggtg ccgagcgcca gatgcatgac gatgccgtca tcgacgccct
      661 gcgtgtggaa cacccaggcg agcgccggca cgatcaccag accgccgccg atgccgagca
      721 ggccggccat gacgccggcg aaggcgccga ccgcgagaca gatcaggagg gtttgcagca
      781 gttcgggcac gggcgcgctt cgtccaggat caggaaaaac aacggaaaac cggcgcggct
      841 tcgcgggcgg acacaggcgg cgggcgccgg catttgtgct agattgcgcg cggtttttcg
      901 caagcccccg cgccgcgcgc cggcgcggcc ccgcattcta acgacagtcc gtcccgcccg
      961 gcgatcgccc ggcggcggtc gacggacatg catccaggga tatccggcat gaaggttcag
     1021 actgtctgcg tcctcggtgg caccggtttc gtcggccgtg ccctcgtctc ccgcctcgcc
     1081 aacagcggct gcaaggtccg ggtgctgacg cgccatccgc agcgccgctg ccgcgagatc
     1141 gaggtggtcc cgaacgtctc gctgctcgcg atcggcgaca cgccgctggc cgggcacttc
     1201 aagggctgcg acgcggtgat caacctcgtc ggcatcctca acgaggccgg cagccagacc
     1261 ttccgcggcg tgcacgtcga actggcgaag cgcgtcgccg ccgcctgcgt cgaggccggc
     1321 gtgccgcggc tgctgcacat gagcgcgctg aacgccgatg ccgtgaacgg cgtgtccgac
     1381 tacctgaaga ccaagggcga gggcgaggac gccgtgcatg cgaccgccgg cctgaaggtc
     1441 acgagcttcc ggccctcggt gatcttcggc tgcgaggatt ccttcttcaa ccgcttcgcc
     1501 gggctgctga agctcgcgcc ggtgctgccg ctcgcctgcc ccgaagcccg cttccagccg
     1561 gtgtacgtgc gcgacgtggc cgcggccttc gcgcaggccc tcgaagacga gcgcaccgtc
     1621 ggcaagcgcc tcgaactcgg cggcccgcgc atctacacgc tgaaggaact cgtcgagttc
     1681 acggcgcgca cgctcggcct gtcgacgctg gtcatcggcc tgccggattc ggtcgcgaag
     1741 ctgcaggcga agctgatgga caagctgccg accaggccct tcagcctcga caactaccgc
     1801 tcgctgcagc aggacagcat cgtcggcggc gaggacggct tcgcgctgct cggcatcgtg
     1861 ccgcgctcgg tcgagggcgt gatgccgcgc gaactgcgcg cccgcgatca ccgcggcccc
     1921 tacgcggcct atcgggccag cgccggacgc tgatcgacgt aatttctatt ctgctgaata
     1981 tattggtgtt ttgttaaggg cgcccatgtg gcgccctttt tgtttgcgtt gcgttcgagt
     2041 gtggtctata aaatttcgtg gatataaatg atgccggcca taatgaaata catcctctgt
     2101 tc
//
LOCUS       DAOQJH010000550         2095 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_42047_length_2095_cov_3.900000, whole genome shotgun sequence.
ACCESSION   DAOQJH010000550 DAOQJH010000000
VERSION     DAOQJH010000550.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2095)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42047_length_2095_cov_3.900000"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..394)
                     /locus_tag="PLQ18_11555"
     CDS             complement(<1..394)
                     /locus_tag="PLQ18_11555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012990547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNE02330.1"
                     /translation="MCRLARRGAALLLVGLCRGLTGVRALWLGSLPAAGARVYFANHA
                     SHLDGLVIWAALPPALRTRTHPVAARDYWDATPLRRWLALQVFGAVLIDRQPATAAGE
                     ADAARADVLAPLLAVLERGEALILFPEGT"
     gene            complement(394..789)
                     /locus_tag="PLQ18_11560"
     CDS             complement(394..789)
                     /locus_tag="PLQ18_11560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02331.1"
                     /translation="MNPMKRMLPALLLVAVTPLAGAGALDGEYRGRADGERHLDLSEH
                     DDGQVSVTMSLDIPRTEGGRCRGEFVAHGLRDGRTITVEPATGKEACRIVIGVQAGQA
                     SISEQGEGCATLRDADCSFSGTLDRIRAR"
     gene            complement(819..1406)
                     /locus_tag="PLQ18_11565"
     CDS             complement(819..1406)
                     /locus_tag="PLQ18_11565"
                     /inference="COORDINATES: protein motif:HMM:NF016239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HNE02332.1"
                     /translation="MNWLAHFYLAGPDPHARVGAMLGDFHRGPVETLADARWRAAVRE
                     HRAIDVWTDAHPLVCASRARLPAPLRRYGGIIIDVAYDHCLARDWANWSAQSLPAFVG
                     AVHAALRADYDRLPPRMQRSIDHLLQGRTLLSYREPAGVARALAGIATRLSRPSPLAA
                     AGPAVTALLPDWAGDFAVFFPELVAFVANRTDAGL"
     gene            complement(1425..>2095)
                     /locus_tag="PLQ18_11570"
     CDS             complement(1425..>2095)
                     /locus_tag="PLQ18_11570"
                     /inference="COORDINATES: protein motif:HMM:NF024948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4129 domain-containing transglutaminase
                     family protein"
                     /protein_id="HNE02333.1"
                     /translation="LGGEPSPGGDYFIVRQSDAHAWAEVWLEGRGWTRIDPTAAIAPE
                     RVERGTASAIPDPDSLSLLSRRGESWVAIALLKLDALEYAWNEWVLAYGPDRQRELLG
                     WLLGWRDIDWRGLILLLVSALAIVGLALALLNRRSRRQGRDPLGRSYARFCARLAAIG
                     LARQASEGPLSYRARIARLRPDLADATGQILVRYAELRFGEHPDPQRLRELNAQLRRF
                     RPRA"
BASE COUNT          304 a          742 c          792 g          257 t
ORIGIN      
        1 gggtgccctc ggggaacagg atcagcgcct cgccgcgttc gagcaccgcg agcagcggtg
       61 ccagcacgtc ggcccgggca gcatcggctt cgccggcagc cgtggccggc tggcggtcga
      121 tcagcaccgc gccgaacacc tgcagcgcca gccagcggcg cagcggcgtc gcatcccagt
      181 aatcgcgcgc ggcgaccgga tgcgtgcggg tgcgcagcgc cggcggcagc gccgcccaga
      241 tcaccaggcc gtcgaggtgg ctcgcgtgat tggcgaagta cacgcgcgcg cccgcagccg
      301 gcaggctgcc gagccacagc gcacgcacgc cggtcaggcc gcggcagagt ccgaccagga
      361 gcagcgccgc accacggcgt gcgagcctgc acatcagcgg gcccggatgc ggtccagcgt
      421 gccgctgaag ctgcagtcgg cgtcgcgcag cgtcgcgcag ccctcgccct gttcgctgat
      481 gctggcctgg ccggcctgca cgccgatgac gatgcggcag gcttccttgc cggtcgcagg
      541 ctcgacggtg atcgtgcggc cgtcgcgcag accgtgcgcg acgaactcgc cgcggcagcg
      601 gccgccttcg gtgcgcggga tgtcgaggct catcgtcacg ctgacctggc cgtcgtcgtg
      661 ctcgctcagg tcgagatggc gttcgccgtc ggcgcgtccg cggtattcgc cgtcgagggc
      721 gccggcaccg gcgaggggcg tgacggcgac gagcagcagg gcaggcagca tgcgcttcat
      781 gggattcatg agattcaaat cctgatccgg atcctgtttc agagccctgc atcggtgcgg
      841 ttcgcgacga aggcgaccag ctccggaaag aagactgcga aatccccggc ccagtccggc
      901 agcagcgccg tcaccgccgg cccggccgcc gccagcggcg acgggcggct caggcgcgtg
      961 gcgatgccgg ccagggcccg ggcgacgccg gcgggttcgc ggtacgaaag cagtgtccgg
     1021 ccctgcagca gatggtcgat gctgcgctgc atgcggggcg gcagacgatc ataatccgcg
     1081 cgcaaggccg cgtgtaccgc cccgacgaag gccggcagcg actgtgcgga ccagttcgcc
     1141 cagtcccggg ccaggcagtg gtcgtaggcc acgtcgatga tgatgccgcc gtagcggcgc
     1201 agcggcgccg gcagccgggc gcggctcgcg catacgagcg gatgcgcatc ggtccagaca
     1261 tcgatcgccc ggtgttcgcg caccgccgcc cgccagcgcg catcggcgag ggtttcgacc
     1321 ggcccgcggt ggaagtcgcc gagcatcgcg ccgacccgtg cgtgcgggtc gggaccggcc
     1381 agatagaaat gtgcgagcca gttcacgggc cgcccgcacg ggcttcaggc gcgcggccgg
     1441 aagcggcgca gctgcgcgtt cagctcgcgc aggcgctgcg ggtccggatg ctcgccgaag
     1501 cgcagttcgg cgtagcgcac gaggatctgt ccggtggcat cggcgagatc cgggcgcagc
     1561 cgggcgatgc gcgcgcgata ggacagcggc ccctcgctgg cctgccgcgc caggccgatc
     1621 gcggccaggc gcgcgcagaa gcgcgcatag ctgcgcccga gcggatcgcg gccctgccgg
     1681 cggctgcgcc ggttcagcag ggcgagcgcg agtccgacga tggcgagcgc cgagaccagc
     1741 agcaggatca ggccgcgcca gtcgatgtcg cgccagccga gcagccagcc gagcagctcg
     1801 cgctggcggt cgggaccgta ggcgagcacc cattcgttcc acgcatattc gagcgcatcg
     1861 agcttgagca gcgcgatcgc gacccagctc tcgccgcggc gcgacagcag cgacaggctg
     1921 tcggggtccg ggatcgccga ggccgtgccg cgctcgacgc gctcgggcgc gatcgccgcg
     1981 gtcgggtcga tgcgcgtcca gccgcggccc tccagccaga cctcggccca ggcgtgggcg
     2041 tcggactggc ggacgatgaa gtaatcgccg cccggtgagg gctcgccgcc gagat
//
LOCUS       DAOQJH010000551         2093 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_42129_length_2093_cov_3.291462, whole genome shotgun sequence.
ACCESSION   DAOQJH010000551 DAOQJH010000000
VERSION     DAOQJH010000551.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2093
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42129_length_2093_cov_3.291462"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..502
                     /locus_tag="PLQ18_11575"
     CDS             <1..502
                     /locus_tag="PLQ18_11575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02334.1"
                     /translation="VWAAPPPALLDQVQRLSELLRDGYASWYREATETQTLQPDAGQT
                     LLLVLFTVEGFGGGNRHTQYLAAFEAGEGAGGKPYYTLLDVIAIGGKGWRAIEKLQAR
                     ATRGRTPIEKLQARATRGRTPGEIVLSLPAKVVGPDDAPNFPGRPASVRLSLRDGRFS
                     KQAGAR"
     gene            complement(521..1663)
                     /locus_tag="PLQ18_11580"
     CDS             complement(521..1663)
                     /locus_tag="PLQ18_11580"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNE02335.1"
                     /translation="MVASEAGAGWFLEGVSDQGGHWLVRITGTPFIVGRVESCDLYLN
                     DTGVSRQHARLEFDGAGRPLLSDEGSTNGTFVNYQRLEARAPQSLNDGDIVHFAKMAF
                     RLVHREQSKAPAPVVEGIEGGTMFQQVAALPETFTAAAHEKLFFDLLAKGAVSPWFQP
                     IISLRHGKQVVAYELLGRGAQPGLSSSPAELLKIAHATGRTVELSDLFRKTGIRRAQE
                     LGLSRVFFNTMPAEMSIPLLRRLLGELRVMAPVLPMVMEVHEQAIADIELMRDVRALL
                     GELNMKLAYDDFGAGQARLVEIMEVPPDVLKFDIGLVRGISSKPQRVQTMVADLVRMA
                     AGMGVRTLAEGIEAQEDSDFCEAAGFDLIQGYLYGRPTPDPLPVAG"
BASE COUNT          361 a          715 c          690 g          327 t
ORIGIN      
        1 ggtttgggcc gcaccaccgc cggcgctgct cgatcaggtg cagcggctga gcgagctgct
       61 gcgtgacggc tacgcaagct ggtatcgcga ggcaaccgaa acgcagacgc tgcagcccga
      121 tgccgggcag acgctgctgc tggtgctgtt cacggtcgag ggtttcggcg gcggcaaccg
      181 tcatacgcag tacctcgcag ccttcgaagc gggcgaaggc gccggcggca agccgtacta
      241 cacgctgctc gacgtgatcg cgatcggcgg caagggctgg cgggcgatcg aaaaactgca
      301 ggcgcgggcc acccgtggcc gcacgcccat cgaaaaactg caggcgcggg ccacccgtgg
      361 ccgcacgccc ggcgagatcg tgctgagcct gccggcgaag gtggtcggcc ccgacgatgc
      421 gccgaacttc ccgggccgcc cggccagcgt gcggctgagc ctgcgcgacg ggcgcttcag
      481 caagcaggcc ggtgcgcgct gaaagagagc gggcgcggtt tcagccggcg acgggcagcg
      541 ggtccggcgt cggccggccg tacagatagc cctggatcag gtcgaagccg gccgcctcgc
      601 agaagtcgga gtcctcctgc gcctcgatgc cctcggcgag cgtgcgcacg cccatgccgg
      661 cggccatgcg cacgaggtcg gcgaccatgg tctgcacgcg ctgcggcttg gagctgatgc
      721 cgcgcaccag gccgatgtcg aacttcagca cgtcgggcgg cacttccatg atctcgacca
      781 gccgcgcctg accggcaccg aagtcgtcgt aggcgagctt catgttcagc tcgccgagca
      841 gcgcgcgcac atcgcgcatc agctcgatgt cggcgatcgc ctgctcgtgg acctccatga
      901 ccatcggcag caccggcgcc atcacgcgca gctcgccgag cagccggcgc agcagcggga
      961 tcgacatctc ggccggcatc gtgttgaaga acacgcgcga cagccccagc tcctgcgcgc
     1021 ggcggatgcc ggtcttgcgg aacaggtcgc tcagttcgac cgtgcggccg gtcgcgtgcg
     1081 cgatcttcag cagttccgcc ggcgagctcg acagccccgg ctgcgcaccg cgcccgagca
     1141 gttcgtaggc aacgacctgc ttgccgtggc gcaggctgat gatcggctgg aaccacggcg
     1201 acaccgcgcc cttcgccagc agatcgaaga acagcttctc gtgggccgca gccgtgaagg
     1261 tttccggcaa cgcggccacc tgctggaaca tcgtgccgcc ctcgatgccc tcgaccaccg
     1321 gcgccggagc cttcgactgc tcgcgatgca ccagccggaa ggccatcttg gcgaaatgca
     1381 cgatgtcgcc atcgttgagc gactgcggcg cgcgggcctc caggcgctgg tagttgacga
     1441 aggtgccgtt ggtgctgccc tcgtcactga gcagcggccg gccggccccg tcgaattcga
     1501 gtcgcgcatg ctggcgcgac accccggtgt cattcaggta caggtcgcag ctctcgacgc
     1561 gcccgacgat gaacggtgtg ccggtgatgc gcaccagcca gtggccgccc tgatcgctga
     1621 cgccttcgag aaaccagcct gcacctgctt cgcttgcaac catttcgccc cccggcgacc
     1681 cggccccgat acggatcgcg cacccggccg atcataggcc agcaggcgtt cccggccacc
     1741 ggcaattgac gtatcccgca gggtctgcga tgcactttcc tgccgccggc cggtgaaggg
     1801 ggtggcacgg ctgcagcgcc ggatgcttcc tgctgtcttc gtgctgcccg catgctgttg
     1861 gggaaagccc gaaaaaaagc ggccggcgca gggggagcgc cggccgagat cgaggggggc
     1921 gacatccgac agatgccgtg atacagaata aacttgtatt ttaatttcgc atagcgtcaa
     1981 tcaattcctg aaccatcatc agtcgaaatt taatgttcaa ctaaatttac atcatgaacg
     2041 atacgccata cacctcatgg ccctgcgttg caaggaatac agccctcgcg cct
//
LOCUS       DAOQJH010000552         2091 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_42219_length_2091_cov_3.938114, whole genome shotgun sequence.
ACCESSION   DAOQJH010000552 DAOQJH010000000
VERSION     DAOQJH010000552.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2091
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42219_length_2091_cov_3.938114"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..504)
                     /locus_tag="PLQ18_11585"
     CDS             complement(<1..504)
                     /locus_tag="PLQ18_11585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3501 family protein"
                     /protein_id="HNE02336.1"
                     /translation="MTALTRADLLSLERYAVERPAFRARVIAHKKRRQLALGAHLTLC
                     FEDRLTIQYQVQEMLRIERIFEPGGIDDELAAYNPLIPDGCNWKATLLLEYPDVDERR
                     AALAVLVGIETRVWFEVAGHARVFAIADEDLERSRADKTWSVHFLRFELDAPMVGALR
                     TGAALSAG"
     gene            complement(516..1862)
                     /locus_tag="PLQ18_11590"
     CDS             complement(516..1862)
                     /locus_tag="PLQ18_11590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019023824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HNE02337.1"
                     /translation="MSAPVNLHREGSLEAPTRHPLDWKNPQFHDEAATLAEMQRVFDI
                     CHGCRRCVSLCNAFPTLFDLVDNSTTQELDGVAKRDYWQVADHCYLCDLCYMTKCPYV
                     PPHPWALDFPHLMLRAKAVRYRQGEVSLRDRVLTSTRTVGALAGIPVVAEAVNRVNAI
                     APLRKVMEAVAGISAEARLPPFAGPSLRSRLKARRRETRRGTPAGRTQGRVVLFATCY
                     CDHNEPHIGEDLVAVFEHNGIPVASTRRERCCGMPKMELGNLDAIEDYKRVNIAELLR
                     WVDAGWDVVAPVPSCVLMFKSELPLLFPDDGEVRRVAQAVFDPFEYLMLRHRAGLLRT
                     DFRQPLGKIAYHVPCHLRVQNMGLKSRELLSLIPGTEVEAIERCSGHNGTYAVKAETH
                     GCSVKIGRPVFRRVQDAKADHYGSDCPMAGTHIAEGVADGRAPEHPLSLLRLAYGL"
     gene            complement(1976..>2091)
                     /locus_tag="PLQ18_11595"
     CDS             complement(1976..>2091)
                     /locus_tag="PLQ18_11595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018946582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HNE02338.1"
                     /translation="ARTAREEGFEEIADWFETLAKAERSHANRFQKALDQL"
BASE COUNT          323 a          709 c          741 g          318 t
ORIGIN      
        1 ccctgccgac aaggccgcgc cggtacgcag cgccccgacc atcggggcgt cgagttcgaa
       61 acgcaggaaa tgcaccgacc aggtcttgtc ggcacggctg cgttcgaggt cctcatcggc
      121 gatcgcgaac acgcgcgcat ggccggcgac ctcgaaccag acccgggttt cgatgccgac
      181 cagcacggcc agcgccgcgc gccgctcgtc gacatccgga tattcgagca gcagcgtcgc
      241 cttccagttg cacccgtccg ggatcagcgg gttgtaggcg gccagctcgt cgtcgatgcc
      301 cccgggctcg aagatgcgct cgatgcgaag catctcctgg acctggtact ggatggtcag
      361 gcggtcctcg aagcagagcg tcaggtgcgc accgagcgcg agctggcggc gcttcttgtg
      421 cgcgatcacg cgggcgcgga aggccgggcg ctcgacggca tagcgttcga gactcagcag
      481 gtcggcgcgg gtcagggcgg tcatggcatt ccccgtcaga ggccgtaggc gagccgcagc
      541 aggctcagcg gatgctccgg ggcgcggccg tcggcgacgc cctcggcgat gtgcgtgccg
      601 gccatcgggc agtcgctgcc gtagtgatcg gcctttgcgt cctgcacgcg ccggaacacc
      661 ggccggccga tcttcaccga acagccatgc gtctcggcct tcacggcgta ggtaccgttg
      721 tggcccgagc agcgctcgat cgcctcgacc tcggtgccgg gaatcaggct cagcagttcg
      781 cggctcttca ggcccatgtt ctgcacgcgc aggtgacagg gcacgtgata ggcgatcttg
      841 ccgagcggct ggcggaagtc ggtgcgcagc agcccggcgc ggtggcgcag catcaggtac
      901 tcgaacggat cgaacaccgc ctgcgcgacg cggcgcacct caccgtcgtc cgggaacagc
      961 agcggcagtt cgctcttgaa catcagcacg cacgagggca ccggcgcgac cacgtcccag
     1021 ccggcatcga cccagcgcag cagctcggcg atgttgacgc gcttgtagtc ctcgatcgca
     1081 tcgaggttgc cgagctccat cttcggcatg ccacagcagc gttcgcggcg cgtgctggcg
     1141 accgggatgc cgttgtgctc gaacaccgcg accagatcct cgccgatgtg cggctcgttg
     1201 tgatcgcagt aacaggtcgc gaacagcacc acccgcccct gcgtgcgccc ggccggcgtg
     1261 ccgcggcggg tttcccggcg gcgcgccttc agccggctgc gcagcgacgg gccggcgaag
     1321 ggcggcagcc gggcttcggc cgagatgccg gcgacggcct ccatgacctt gcgcagcggc
     1381 gcaatggcat tgacgcggtt gacggcttcg gcgaccaccg gaatgccggc gagcgcgccg
     1441 accgtgcgcg tcgaggtcag cacgcggtcg cgcaggctga cctcgccctg gcggtagcgc
     1501 acggccttgg cgcgcagcat caggtgcggg aaatcgagcg cccacggatg cggcggcacg
     1561 tacgggcatt tggtcatgta gcagaggtcg cacagatagc agtgatcggc gacctgccag
     1621 taatcacgct tcgcgacacc gtcgagttcc tgcgtcgtgc tgttgtcgac cagatcgaac
     1681 agggtcggga aggcattgca gaggctcacg cagcgccggc agccgtggca gatgtcgaac
     1741 acgcgctgca tctccgcgag cgtcgcggcc tcgtcgtgga actgcgggtt cttccagtcc
     1801 agcggatgtc gcgtcggggc ttcgaggctg ccttcgcggt ggaggttgac gggcgcggac
     1861 atgggaaggc ctcctcgtgc agcgtgagcc gcaccggcac gaaacgcatc ggggccgcgt
     1921 cacggcggcc ccgaactcgc gaacggacgg gacaccggcg cgctcgcgcc ggcgctcaga
     1981 gctgatcgag cgccttctgg aagcggttcg cgtgcgagcg ctcggccttg gcgagcgtct
     2041 cgaaccagtc ggcgatctcc tcgaagccct cctcgcgggc ggtgcgcgcc a
//
LOCUS       DAOQJH010000553         2087 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_42386_length_2087_cov_3.468504, whole genome shotgun sequence.
ACCESSION   DAOQJH010000553 DAOQJH010000000
VERSION     DAOQJH010000553.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2087
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42386_length_2087_cov_3.468504"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..358)
                     /locus_tag="PLQ18_11600"
     CDS             complement(<1..358)
                     /locus_tag="PLQ18_11600"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNE02339.1"
                     /translation="MSAPATPAVRTQALTRSFGDGDESVQVLRGVDYQAGFGALNYLV
                     GPSGCGKTTLLSIVAGLLEPGDGDLEVLGERPQRFGARARAAFRRERLGFVFQQYHLL
                     PALTAAENAAVTLIAAG"
     gene            complement(355..1494)
                     /locus_tag="PLQ18_11605"
     CDS             complement(355..1494)
                     /locus_tag="PLQ18_11605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNE02340.1"
                     /translation="MNYIAWQMLTGDRAKYFALVFTIAFASFLLVQQVSIFVGILERT
                     ASQIVDVAEPDLWVMDPATRYFDEVQALKSNDLYRVRGVPGTQWAVPLYKGNARMRAA
                     DGSFRQVILLGLDDATLTGVPRRMLLGSAEALAEPDAVILDRAGYLFFFPGEPLVLGR
                     EFELNDRRAKLVGIAEASAPFVTFPVVFTRYSNAVTYIGRERNTLSFVLAKAAPGVPV
                     AELAARIGAQTGLAAQSVDGFRRQTIRFYLANTGIPVNFGITIVLAVLIGMVVAGQTF
                     YLFTLENLKQYGVLKAIGVSDGRIVAMILLQACVVGLIGYGLGMGLVAAFFEVMLGYL
                     PVRGIVVQPVAAMVTGAVVLAIVALASLLSVRRVLVLEPAAVFRG"
     gene            complement(1673..>2087)
                     /locus_tag="PLQ18_11610"
     CDS             complement(1673..>2087)
                     /locus_tag="PLQ18_11610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02341.1"
                     /translation="YPDYFEVETLVKIASFAGRASEERQNALGLGVGRCFRAVNAVVH
                     AALAAGDLPADGLPPERIVFAIASVSIGSEIMGRLPALRLLAGIDELAWTVRANQCVL
                     LDGLGWRPLSRDFDMAAVDRRLRAEVFPEATWLQV"
BASE COUNT          365 a          727 c          732 g          263 t
ORIGIN      
        1 tgccggcggc gatcagcgtc accgcggcgt tctcggccgc ggtcagcgcc ggcagcaggt
       61 ggtactgctg gaacacgaag ccgagccgct cgcggcggaa ggccgcgcgg gcgcgggcgc
      121 cgaagcgctg cggacgctcg ccgagcactt cgagatcgcc gtcgccgggt tcgagcaggc
      181 cggcgacgat cgacagcagc gtggtcttgc cgcagccaga cgggccgacc agatagttca
      241 gcgcgccgaa tccggcctga tagtcgacgc cgcgcagcac ctgcaccgac tcgtcgccat
      301 cgccgaagct gcgcgtcagc gcctgcgtgc gcacggccgg ggtcgccggc gcgctcatcc
      361 gcggaacacc gcggccggct ccagcacgag cacgcggcgc accgacagca ggctcgcgag
      421 cgcgacgatc gcgagcacga cggcgccggt gaccatggcc gcgaccggct gcacgacgat
      481 gccgcgcacc ggcagatagc cgagcatgac ctcgaagaag gccgcgacca ggcccatgcc
      541 gaggccgtag ccgatcaggc cgaccacgca ggcctgcagc aggatcatcg cgacgatgcg
      601 cccgtccgag acaccgatcg ccttcagcac gccgtactgc ttcaggtttt cgagcgtgaa
      661 caggtagaag gtctggccgg cgaccaccat gccaatcagc accgccagca cgatggtgat
      721 gccgaagttg accggaatgc cggtgttggc gagatagaag cggatggtct ggcgccggaa
      781 accgtcgacg ctctgcgccg ccagcccggt ctgcgcgccg atgcgcgcgg ccagctccgc
      841 gaccggcacg cccggcgcgg ccttcgcgag cacgaaggac agcgtgttgc gctcgcggcc
      901 gatataggtg acggcgttcg aatagcgcgt gaacaccacc gggaaggtca cgaacggcgc
      961 gctggcctcg gcgatgccga cgagcttcgc gcgccggtcg ttcagctcga actcgcgccc
     1021 gagcacgagc ggctcgccgg ggaaaaagaa caggtagccg gcgcgatcga ggatcaccgc
     1081 atccggctcg gccagcgcct cggcggagcc gagcagcatg cgccgcggca cgccggtcag
     1141 cgtcgcatcg tcgaggccga gcaggatcac ctgccggaag ctgccgtcgg cggcgcgcat
     1201 gcgggcattg cccttgtaga gcggcacggc ccactgcgtg ccgggcacgc cgcgcacacg
     1261 gtacaggtcg ttcgacttca gcgcctgcac ctcgtcgaag tagcgcgtcg ccgggtccat
     1321 gacccacagg tccggttcgg cgacatcgac gatctgactg gccgtgcgtt cgaggatgcc
     1381 gacgaagatc gacacctgct gcacgagcag gaaggacgcg aacgcgatcg tgaacaccag
     1441 cgcgaagtac ttggcgcgat cgccggtcag catctgccag gcgatgtagt tcatgccggt
     1501 gaccccgcgg tacttgctgc gcctgctgca ctgaatgata tttttcgact ttcagtcaaa
     1561 tcatgagcac acgaaagccc gccggacacc gccggccgga ccgggccgga cgcagcggat
     1621 gcccgaaggc gcagcggccc cgggagaagg cagggatgcg gattgcgcgg actcagacct
     1681 gcagccatgt cgcctccgga aacacctcgg cgcgcaggcg ccggtcgacg gcggccatgt
     1741 cgaagtcgcg cgacagcggc cgccagccga ggccgtcgag cagcacgcac tggttggcgc
     1801 gcacggtcca ggccagctcg tcgatgccgg cgagcagccg cagcgccggc agccggccca
     1861 tgatctcgct gccgatgctg accgaggcga tcgcgaacac gatgcgctcc ggtggcaggc
     1921 cgtcggccgg cagatcgccg gcggccagcg ccgcatgcac gacggcattg acggcgcgga
     1981 agcagcgtcc gacgcccagg ccgagcgcgt tctggcgttc ctcgctggcg cggccggcga
     2041 agctcgcgat cttcacgagg gtctcgacct cgaagtaatc cgggtac
//
LOCUS       DAOQJH010000554         2080 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_42693_length_2080_cov_2.856790, whole genome shotgun sequence.
ACCESSION   DAOQJH010000554 DAOQJH010000000
VERSION     DAOQJH010000554.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2080
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42693_length_2080_cov_2.856790"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /locus_tag="PLQ18_11615"
     CDS             <1..241
                     /locus_tag="PLQ18_11615"
                     /inference="COORDINATES: protein motif:HMM:NF020037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNE02342.1"
                     /translation="PPVVATRVGPAAAEALGDDRIRSVDYPLHIDGELHHYAARILRL
                     AAEDGTVEGVLWLARDITDRKRIEQALFQERNARR"
     gene            238..1881
                     /locus_tag="PLQ18_11620"
     CDS             238..1881
                     /locus_tag="PLQ18_11620"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNE02343.1"
                     /translation="MTLAAIGDGVLVTDEEARLEYLNPVAERLCDCRLAEVRGWPLAA
                     VLRIEAEAGSELGSDPATQCLADNRPVGPALHARLMAADGRERHVEISCAPIHDPAGR
                     PTGTVTVMHDVSERHRLTQQIRHQAAHDPLTGLINRREFELRLDAACRIADDQNRTHA
                     LLCIDLDRFKIVNDSCGHLAGDELLRQVARLMTEHTRQRDVLARMGGDEFALLLEDCP
                     LPKAAEIAGALVSAVGEFRFVHGTQAFSIGASIGVAPVPPGNGGPQVVLSRADRACYA
                     AKDAGRNCVSLANEDGDAASSGTRHWHERLETLLDGGSQDYELHVQPASALYAGLPPY
                     REVLLRLRDDAGRLVPPGAFLPVADRYRLAAGLDRFVLDALCRRLAAGDGGADGLHAM
                     NLSVATLEDGGFAGHVRTALQRHGLAPARLGFEIEEAAVHGRLAQAGEQIGALRALGC
                     RVTLDRCGSGLAAFDCLKVLRVDALKIDGSRLEAEGSHPVDTVYAEAIVRLARLLGLH
                     TIASRIETTGQHSGAVVLGIDYVQGYGVERSQRLGGRDD"
     gene            1891..>2080
                     /locus_tag="PLQ18_11625"
     CDS             1891..>2080
                     /locus_tag="PLQ18_11625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02344.1"
                     /translation="MRRRALSRLHPLQGWGGVTGEDEAFSLRVCNARDYRYHGADMGI
                     LVSRGRMQTDDRELFPRAK"
BASE COUNT          302 a          779 c          727 g          272 t
ORIGIN      
        1 gccgcctgtc gtcgccaccc gcgtcggacc ggccgccgcc gaagccctcg gtgatgaccg
       61 catccgctcg gtcgactatc cgctgcacat cgacggcgaa ctgcatcatt acgcggcgcg
      121 catcctgcgg ctggcggccg aggacggcac cgtcgagggc gtgctgtggc tcgcgcggga
      181 catcaccgac cgcaagcgca tcgagcaggc gctgttccag gaaaggaacg cgcgcaggtg
      241 acgctggcgg cgatcggcga cggcgtgctg gtcaccgatg aggaagcccg cctcgaatac
      301 ctgaatccgg tcgccgaacg gctgtgcgac tgccggctgg ccgaggtgcg cggctggccg
      361 ctcgccgcgg tgctgcgcat cgaggccgaa gccggcagcg aactcggcag cgatccggcc
      421 acgcagtgtc tggccgacaa ccgcccggtc ggccccgccc tgcatgcccg gctcatggcc
      481 gcggacgggc gcgagcgcca cgtcgagatc agctgcgcac cgatccacga cccggccggc
      541 cgcccgaccg gcaccgtgac ggtcatgcac gacgtgagcg aacgccaccg cctgacccag
      601 cagatccgcc accaggccgc ccacgatccg ctgaccggcc tgatcaaccg ccgcgagttc
      661 gagctgcgcc tcgacgccgc ctgccgcatc gccgatgacc agaaccgcac gcacgcgctg
      721 ctgtgcatcg acctcgaccg cttcaagatc gtcaacgaca gctgcgggca tctggccggc
      781 gacgaactgc tgcgccaggt cgcgcggctg atgaccgagc acacccgcca gcgcgacgtg
      841 ctcgcgcgca tgggcggcga cgaattcgca ctgctgctcg aagactgtcc gctgccgaag
      901 gccgccgaga ttgccggggc actggtcagc gcggtcggcg agttccgctt cgtgcatggc
      961 acgcaggcgt tctcgatcgg cgccagcatc ggtgtcgctc cggtgccgcc cggcaacggc
     1021 gggccgcagg tggtgctgag ccgcgccgac cgcgcctgct acgcggccaa ggacgccggg
     1081 cgcaactgcg tgtcgctggc caatgaggac ggcgatgccg cgagcagcgg cacccgccac
     1141 tggcacgagc gcctcgaaac cctcctcgac ggcggctcac aggactacga actgcacgtg
     1201 cagccggcct cggcgctgta cgccggcctg ccgccgtacc gggaggtgct gctgcgcctg
     1261 cgcgacgacg ccggccggct ggtgccgccg ggcgccttcc tgccggtcgc cgaccgctac
     1321 cggctcgccg ccggcctcga ccgcttcgtg ctcgatgcgc tgtgccgccg gctcgcggcg
     1381 ggcgatggcg gcgccgacgg cctgcacgcc atgaacctga gcgtcgccac gctggaggac
     1441 ggcggctttg ccgggcacgt gcgcacggcg ctgcagcgcc acggcctggc gccggcccgg
     1501 ctcggcttcg agatcgagga agccgccgtg cacggccggc ttgcgcaggc cggcgagcag
     1561 atcggcgcgc tgcgcgccct cggctgccgg gtcaccctcg atcgctgcgg cagcggcctc
     1621 gcggccttcg actgcctgaa ggtgctgcgg gtcgatgcgc tgaagatcga cggcagccgc
     1681 ctggaggccg aaggcagcca cccggtcgac accgtctacg ccgaagccat cgtccgcctg
     1741 gcccgcctgc tcggcctgca cacgatcgcc agccgcatcg aaaccaccgg ccagcacagc
     1801 ggcgcggtcg tgctcggcat cgattatgtg caggggtatg gggtggagcg gtcgcagcgg
     1861 ctcggtggcc gtgacgactg aacagcgtcg atgcgccggc gcgcgctcag caggctgcac
     1921 cctttacaag gctggggcgg cgtcaccggc gaggacgaag ccttttctct gcgcgtctgc
     1981 aacgcccgcg attaccgcta ccacggcgcc gacatgggca ttctcgtcag ccgcggccgc
     2041 atgcagaccg acgaccgcga actgttcccg cgcgcgaagc
//
LOCUS       DAOQJH010000555         2078 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_42770_length_2078_cov_3.326248, whole genome shotgun sequence.
ACCESSION   DAOQJH010000555 DAOQJH010000000
VERSION     DAOQJH010000555.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2078
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42770_length_2078_cov_3.326248"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..488
                     /locus_tag="PLQ18_11630"
     CDS             <1..488
                     /locus_tag="PLQ18_11630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018230878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNE02345.1"
                     /translation="APRMLGVALRILVRRDRAEDAVQEAFVKVWQRASDYSATAGSPL
                     AWLTAIVRHQALDLLRSDKRRHTESTDDDDEDGPGRLDPPDERPDPLGLLEQAIDGLR
                     LRVCFDVVPEQQRRCLALAYYHGLTHSELAEHIAAPIGSVKVWLRRGLDRLRRCLEAA
                     P"
     gene            485..1270
                     /locus_tag="PLQ18_11635"
     CDS             485..1270
                     /locus_tag="PLQ18_11635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008063771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HNE02346.1"
                     /translation="MNYDRPPLIDHLAAAYVLGTLSARARRRFERLCRTLPSAADAVR
                     DWERRLGGLAEPVPPVAPSPQLWHAIERRTLGVREHPETARQPEARTRRERSESSRLG
                     DWLRPLLGFACGLLLAVGLVRLYPQGFVPGEPVDETALPASYIGLLTNAEGQPVLLAS
                     IRRHGRELQFKVLKPLAVPAGKVAVLWALAPNAAPAVVGVVPFAGGETAAPLAKATLT
                     LAGSAEQLLSNVPKLALSFESAPPAAGAAPSPFALEGNCVKLW"
     gene            1311..>2078
                     /locus_tag="PLQ18_11640"
     CDS             1311..>2078
                     /locus_tag="PLQ18_11640"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006397376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNE02347.1"
                     /translation="MTPQAVVTEAVLDLPGGAQRITAPAAIRTAYRPDIDGLRAVAVL
                     LVVIHHAFPQSGGGFIGVDVFFVISGYLITGILAGDIRQSRFRLAGFYARRIRRIYPA
                     LLVVLLASLLAGWFLMLPNELATLGRHVAGGSGFVANVLYWLQSGYFDRQAELQPLLH
                     LWSLGVEEQFYIVWPLLLAGLMRLPPRRCLQAVGALLLLSLLGCIVVSEYDLSAAFFL
                     PHTRFWELAAGGWLALQQAGQRPAADGLRHGRDGRPRD"
BASE COUNT          248 a          789 c          733 g          308 t
ORIGIN      
        1 gcgcgccgcg catgctcggc gtggccctgc gcatcctcgt gcgccgcgac cgcgccgagg
       61 acgccgtgca ggaagccttc gtgaaggtct ggcagcgcgc gagcgactat tcggcgaccg
      121 ccggctcgcc gctggcgtgg ctgacggcga tcgtgcgtca tcaggcgctc gacctgctgc
      181 gcagcgacaa gcgccggcac accgaatcga ccgacgacga cgacgaggac ggccccggcc
      241 gcctcgatcc gcccgacgag cggcccgatc cgctcggcct gctcgaacag gcgatcgacg
      301 gcctgcgcct gcgcgtgtgc ttcgatgtcg tgcccgagca gcagcgtcgc tgtctggcgc
      361 tcgcgtacta tcacgggctc acgcattcgg agctggccga gcacatcgcc gccccgatcg
      421 gcagcgtcaa ggtctggctg cgccgcggcc tcgaccggct gcgccgctgc ctggaagccg
      481 ccccatgaac tacgaccgtc cgccgctgat cgatcacctc gccgctgcct atgtgctcgg
      541 cacgctgtcg gcgcgcgcgc gccggcgctt cgagcggctc tgccgcacgc tgccttcggc
      601 cgccgacgcc gtgcgcgact gggagcgccg gctcggcggc ctcgccgaac cggtgccgcc
      661 ggtcgcaccg tcgccgcagc tctggcacgc gatcgagcga cgcacgctcg gcgtgcgcga
      721 acacccggaa accgcccgtc agcccgaagc gcgcacgcgc cgggaacgca gcgaaagcag
      781 ccggctcggc gactggctgc ggccgctgct cggtttcgcc tgcggcctgc tgctcgccgt
      841 cgggctggtg cggctgtacc cgcagggctt cgtgccgggc gagccggtcg acgagaccgc
      901 gctgccggcg agctacatcg ggctgctgac caacgccgaa ggccagccgg tgctgctcgc
      961 gagcatccgc cgccacggcc gcgagctgca gttcaaggtg ctgaaaccgc tggcggtgcc
     1021 ggccggcaag gtggcagtgc tgtgggcgct ggcgccgaac gccgccccgg ccgtggtcgg
     1081 cgtcgtgccg ttcgcgggcg gcgagaccgc cgcgccgctc gcgaaggcga cgctgacgct
     1141 cgccggcagc gccgagcagt tgctgagcaa cgtgccgaag ctcgcgctga gcttcgaatc
     1201 cgccccgccg gccgccggcg ccgccccgtc gccgttcgcg ctcgaaggca actgcgtgaa
     1261 gctctggtag gtcgtccgca gccgcacgcg ctgcagggca gggaaccggc atgacgccgc
     1321 aggcggtggt gacggaggcg gttctggact tgcccggcgg ggcgcagcgg atcactgcgc
     1381 cggcggcgat ccgcacggcc taccgccccg acatcgacgg cctgcgcgcc gtcgccgtgc
     1441 tgctggtggt gatccatcac gcctttccgc agagcggcgg cggcttcatc ggcgtcgacg
     1501 tgttcttcgt gatctccggc tacctgatca ccggcatcct cgccggcgat atccgccagt
     1561 cgcgctttcg cctcgccggc ttctacgccc ggcgcatccg ccgcatctat ccggcgctgc
     1621 tcgtggtgct gctggcgagc ctgctcgccg gctggttcct gatgctgccg aacgaactcg
     1681 ccaccctcgg ccggcacgtc gccggcggca gcggcttcgt cgccaatgtc ctgtactggc
     1741 tgcagtccgg ttacttcgac cggcaggccg aactgcagcc gctgctgcac ctgtggtcgc
     1801 tcggcgtcga ggagcagttc tacatcgtct ggccgctgct gctggcgggg ctgatgcgcc
     1861 tgccgccgcg ccgctgcctg caggccgtcg gcgcgctgct gctgctgtcg ctgctcggct
     1921 gcatcgtcgt cagcgaatac gacctgagcg cggccttctt cctgccgcac acccgcttct
     1981 gggaactcgc ggccggcggc tggctggcgc tgcagcaggc cggacagcgg ccggccgcag
     2041 acggcctgcg ccacggccgc gacggccggc cccgagac
//
LOCUS       DAOQJH010000556         2076 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_42855_length_2076_cov_3.752598, whole genome shotgun sequence.
ACCESSION   DAOQJH010000556 DAOQJH010000000
VERSION     DAOQJH010000556.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2076
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42855_length_2076_cov_3.752598"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="PLQ18_11645"
     CDS             <1..213
                     /locus_tag="PLQ18_11645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02348.1"
                     /translation="VHELFRKAVHRLSRCAATPVGAGYAVNPDAPERGEALHLSGAAP
                     DVPVTVRNRGRDRRRILPGSRPIGSK"
     gene            400..1506
                     /locus_tag="PLQ18_11650"
     CDS             400..1506
                     /locus_tag="PLQ18_11650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012074187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNE02349.1"
                     /translation="MSTQSGGDAFVRFREVRKTYDGEHLVVKNLNLDIGRGEFLTLLG
                     PSGSGKTTALMMLAGFETPTHGEILLDGKPLHNLPPYRRDIGMVFQNYALFPHMTVAE
                     NLAFPLSVRKLPKADIAQKVARALDMVRLGALAGRYPAQMSGGQQQRVALARALVFEP
                     KLVLMDEPLGALDKQLREHMQLEIKQLHAQLGLTVVYVTHDQSEALTMSDRVAVFNDG
                     VIQQIDAPETLYEHPQNSFVAHFIGENNRLAGEVQTIDDRSCTVRLDDGPLVQARAVN
                     VGGVGSRTSASIRPERLRLAGAAQGADNVFEARIRDFIYLGDHLRVRLEFAGSREFTV
                     KVPVSQFDPRWRAGDTLAVGWSADDLRALDPVAA"
     gene            1613..>2076
                     /locus_tag="PLQ18_11655"
     CDS             1613..>2076
                     /locus_tag="PLQ18_11655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013318163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNE02350.1"
                     /translation="MQSTKFQSKRILSALLAAGLCSAATLAGAADLTVISFGGSNQKA
                     QEVAFYKPFQASGAGTVVAGEYNGEIAKIKAMVETGNVSWDIVEVEGPELLRGCSEGL
                     YEKLDWAKLGDKSKFVPAAVSECGAGIFVWSTALAYNADKLARAPASWADFW"
BASE COUNT          336 a          726 c          690 g          324 t
ORIGIN      
        1 gtccacgagc tgttccggaa ggctgttcat cgtttgtccc gttgcgccgc caccccggtc
       61 ggtgccggct acgctgtcaa cccggacgcc ccggagcggg gcgaagcgct gcacctgtct
      121 ggtgcagcgc cggatgtgcc ggtcacggtg cgcaaccgtg gacgcgacag gcggcggatt
      181 ttaccgggtt cccgtccgat tggctcgaaa taataaacgc ttcattgcgg aacaacgggc
      241 gtatcgcagg gttgccacgg tttatgtggc tttgcgttgt tgagtttttt aaacactgtt
      301 agcctgcacc gcacatgata actgcgcttc ggccgagggg caccgaccgg tgtccttgac
      361 agccgcagcc gtgacagccc ccagccaaag aggcgccaca tgagcacgca gtccggcggc
      421 gacgccttcg tccggttccg cgaggtccgc aagacctacg acggcgaaca cctggtcgtg
      481 aagaacctga acctcgacat cggccgcggc gagttcctga cgctgctcgg gccctcgggt
      541 tccggcaaga ccacggccct gatgatgctg gccggtttcg agacgccgac gcacggcgag
      601 atcctgctcg acggcaagcc cctgcacaac ctgccgccgt accggcgcga catcggcatg
      661 gtgttccaga attacgcgct gttcccgcac atgacggtgg ccgagaacct cgccttcccg
      721 ctgtcggtgc gcaagctgcc gaaggccgac atcgcgcaga aggtcgcgcg cgcgctcgac
      781 atggtccgcc tcggtgcgct ggccgggcgc tacccggcgc agatgtccgg cggccagcag
      841 cagcgcgtgg cgctcgcccg ggcgctggtg ttcgagccga agctggtgct gatggacgag
      901 ccgctcgggg cgctcgacaa gcagttgcgc gagcacatgc agctggagat caagcagctg
      961 cacgcgcagc tcggcctgac cgtcgtctac gtcacgcacg accagagcga ggcgctgacg
     1021 atgtcggacc gcgtcgcggt gttcaacgac ggcgtgatcc agcagatcga cgcccccgaa
     1081 accctgtacg aacacccgca gaactccttc gtcgcgcact tcatcggcga gaacaaccgc
     1141 ctggccggcg aggtgcagac gatcgatgac cgcagctgca ccgtgcgcct cgacgacggt
     1201 ccgctggtgc aggcgcgggc cgtcaacgtc ggcggcgtcg gcagccgcac ctcggcctcg
     1261 atccggcccg agcgcctgcg tctggccggc gccgcgcagg gcgccgacaa cgtcttcgag
     1321 gcgcgcatcc gcgatttcat ctatctcggc gatcacctgc gcgtgcgtct cgagttcgcc
     1381 ggcagccggg aattcacggt caaggtgccg gtgtcgcagt tcgacccgcg ctggcgtgcc
     1441 ggcgacaccc tcgccgtcgg ctggtcggcc gacgacctgc gcgcgctcga tccggtcgcc
     1501 gcctgatcca ccgcttccgc aacaacgacg gacggcggcc agcccggcgc cgcccgcagc
     1561 cgccggtggc gcagcacgcc ggtccttcaa ccctcagggg gagcagcaaa tcatgcagtc
     1621 cacgaagttc cagtccaagc gcatcctgtc cgccctgctc gccgccggcc tgtgctcggc
     1681 cgcgacgctg gccggggccg ccgacctgac cgtcatctcc ttcggcggca gcaaccagaa
     1741 ggcgcaggaa gtggccttct acaagccctt ccaggcgagc ggcgccggca ccgtcgtcgc
     1801 cggcgagtac aacggcgaga tcgccaagat caaggcgatg gtcgagaccg gcaacgtctc
     1861 gtgggacatc gtcgaggtcg aaggccccga gctgctgcgc ggctgctccg agggcctcta
     1921 cgagaagctc gactgggcca agctcgggga caagtcgaag ttcgtgccgg cggcggtcag
     1981 cgagtgcggt gccggcatct tcgtgtggtc gaccgcgctc gcctacaacg ccgacaagct
     2041 cgccagggcc ccggcctcgt gggcggactt ctggga
//
LOCUS       DAOQJH010000557         2076 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_42861_length_2076_cov_3.358238, whole genome shotgun sequence.
ACCESSION   DAOQJH010000557 DAOQJH010000000
VERSION     DAOQJH010000557.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2076
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42861_length_2076_cov_3.358238"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..215
                     /locus_tag="PLQ18_11660"
     CDS             <1..215
                     /locus_tag="PLQ18_11660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02351.1"
                     /translation="VNGTGIRITNENTPGLYKEMGADSQLHAVWFQIDPSSGGITPVH
                     YNETGYKVGTNASFLSSGCVNSALGI"
     gene            220..543
                     /locus_tag="PLQ18_11665"
     CDS             220..543
                     /locus_tag="PLQ18_11665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02352.1"
                     /translation="MHGTPEPRHPAAFWRPNAFSRLHWQASDEAGEFHLVHDETSGRT
                     HFLTPLGAWLLQRLAEAPAGCAQLQLELRADCDDPDAPGLDQALADALDLLAAELLIE
                     PCPPS"
     gene            540..1484
                     /locus_tag="PLQ18_11670"
     CDS             540..1484
                     /locus_tag="PLQ18_11670"
                     /inference="COORDINATES: protein motif:HMM:TIGR04352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HprK-related kinase A"
                     /protein_id="HNE02353.1"
                     /translation="MTACLGELSRAELRARLADNALRLRIGPFAYALGSEWPGLADWL
                     APLYARYPRFPDDECADFRVRLRWRGLHGRLRRELAVEFEGERLAYRPFGPDYAPPHL
                     EWGLNWGVAFHAGRLLMLHAAVVARGERALLLPAQPGSGKSTLAAALGFRGWRFLSDE
                     FCLIDLQRFELLPFPRLVALKNASVPAMRAHAPAAVIGRDFPNTAKGCLAHLQPPPGS
                     IEAMDRPAVPAWIVFPQFRAGSRARLEAVPPERTMARLCTNAFNYEHTGLTGFETVAR
                     LVSGCPARALEFGTLDDAIAAIDALADGREPLSAAGAR"
     gene            complement(1462..>2076)
                     /locus_tag="PLQ18_11675"
     CDS             complement(1462..>2076)
                     /locus_tag="PLQ18_11675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02354.1"
                     /translation="PAPRACGDWACPSKRRASRPRTPPIMSSSTPLLACLLLATAGSA
                     FAASFDCTRAGTLVEQAICSNPELSDLDDAMSNAYREALAQAADAAVVQATQRRWLSE
                     VRNPCRNTGCLRSAYRTRLRELAAVSPAVQPARELRIVGRVRYGTLDSAIETESGRSY
                     GFGSDSAIGARILDTCGDRGVCEVSGFVDADDTLTRVLSVRRLR"
BASE COUNT          278 a          753 c          743 g          302 t
ORIGIN      
        1 ccgtcaacgg caccggcatc cggatcacca acgagaacac ccccggcctg tacaaggaaa
       61 tgggggcgga cagccagctg cacgcggtct ggttccagat cgatccgtcc agcggcggca
      121 tcacgccggt ccactacaac gagaccggtt acaaggtcgg caccaacgcc tcgttcctgt
      181 cgagcggctg cgtgaactcc gcgctcggca tctgagcccg tgcacggcac tcccgaaccc
      241 cgccatccgg ccgccttctg gcggccgaat gctttttcgc gcctgcactg gcaggcgagc
      301 gacgaggccg gcgaattcca cctcgtccac gacgagacct cgggccgcac gcacttcctg
      361 acgccgctcg gtgcctggct gctgcagcgg ctcgcggagg cgccggccgg ctgcgcgcag
      421 ctgcagctcg aactgcgcgc cgactgcgac gatccggatg cgcccggcct cgaccaggcg
      481 ctcgccgatg cgctcgacct gctcgccgcc gaactgctga tcgaaccctg tccgccgtca
      541 tgaccgcctg cctcggcgag ctgagccgcg ccgaactccg ggcgcggctg gccgacaatg
      601 cgctgcgcct gcgcatcggc ccgttcgcgt acgcgctcgg ctccgaatgg ccggggctgg
      661 ccgactggct ggcgccgctc tatgcgcgct atccgcggtt tccggacgat gaatgcgcgg
      721 atttccgcgt gcgcctgcgc tggcgcgggc tgcacgggcg gctgcggcgc gagctggcgg
      781 tcgagttcga gggcgagcgg ctggcctacc ggccgttcgg tcccgactac gcgccgccgc
      841 acctcgaatg ggggctgaac tggggggtgg ccttccacgc cggccggctg ctgatgctgc
      901 acgcggcggt ggtcgcgcgc ggtgagcgcg cgctgctgct gccggcccag cccggcagcg
      961 gcaagagcac gctcgcggcc gcgctcggat tccgcggctg gcggtttctg tccgacgagt
     1021 tctgcctgat cgacctgcag cgcttcgagc tgctgccgtt cccgcggctg gtggcgctga
     1081 agaatgcctc ggtgccggcg atgcgcgcgc acgcgccggc ggccgtgatc ggccgggatt
     1141 ttccgaacac cgccaagggc tgtctcgcgc acctgcagcc gccgcccggc agcatcgagg
     1201 ccatggaccg cccggccgtg ccggcgtgga ttgttttccc gcagttccgg gccggcagcc
     1261 gcgcacggct cgaagccgtg ccgccggagc gcacgatggc ccgtctctgc acgaatgcct
     1321 tcaactacga gcacaccggc ctcaccggct tcgaaaccgt ggcgcggctg gtgagcggct
     1381 gtccggcgcg ggccctcgaa ttcggcacgc tcgatgacgc catcgccgcc atcgacgcgc
     1441 tcgcggatgg ccgggagccg ctcagcgcag ccggcgcacg ctgagcacgc gcgtcagcgt
     1501 gtcgtcggca tcgacgaagc cgctgacctc gcagacgccg cgatccccgc aggtatcgag
     1561 gatgcgcgcg ccgatcgccg agtcgctgcc gaagccgtag ctgcgcccgc tttcggtctc
     1621 gatcgccgaa tcgagcgtgc cgtagcgcac gcgcccgacg atgcgcagct cccgcgccgg
     1681 ctgcaccgct ggcgacaccg cggcgagttc gcgcaggcgc gtgcggtagg cgctgcgcag
     1741 gcagccggtg ttgcggcagg gattgcgcac ctcgctcagc cagcgccgct gcgtggcctg
     1801 caccacggcc gcatcggcgg cctgcgccag cgcctcgcgg taggcgttgc tcatcgcgtc
     1861 gtcgagatcg gagagttccg ggttgctgca gatcgcctgt tcgaccagcg tgccggcgcg
     1921 cgtgcagtcg aagctcgcgg caaaggcgct gccggcggtg gcaagcagca ggcaggcgag
     1981 cagcggggtg gaggaggaca tgatcggcgg agtacgcgga cgcgaggcgc ggcgtttgga
     2041 aggacaggcc cagtcgccgc aagcgcgtgg cgctgg
//
LOCUS       DAOQJH010000558         2075 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_42902_length_2075_cov_4.061881, whole genome shotgun sequence.
ACCESSION   DAOQJH010000558 DAOQJH010000000
VERSION     DAOQJH010000558.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2075
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42902_length_2075_cov_4.061881"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(79..399)
                     /locus_tag="PLQ18_11680"
     CDS             complement(79..399)
                     /locus_tag="PLQ18_11680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02355.1"
                     /translation="MNRPNRMLALFAAAALLSGIGLAQAKTETIVTTITNPDGTVTRQ
                     VEQREVPNSVPDQSFEWADRDGNGCIDPREAKDMGILNFAKADTARRGCLGRAEYEHA
                     LISQ"
     gene            676..1182
                     /locus_tag="PLQ18_11685"
     CDS             676..1182
                     /locus_tag="PLQ18_11685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006221276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNE02356.1"
                     /translation="MLADAMTAEIPRLRRYARALTGDPERADDLVQDCLERAWTRQHH
                     YDGSSELRPWLFTILHNLHANAARSYHRRPAFVDVDALAEAAPVAPNHDGELALRDLA
                     QAMDRLSDEQRAVLLLIGVEELTYPQAAAVLGVPVGTVMSRLSRARERLRQLLAGADD
                     GPRLRRVK"
     gene            1182..1997
                     /locus_tag="PLQ18_11690"
     CDS             1182..1997
                     /locus_tag="PLQ18_11690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020704537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HNE02357.1"
                     /translation="MNGESGRRIAEAELHAWLDGRLEPERRAAVEDWLAANPDEAERL
                     ASYHTAEAGLRELYAPVLEEPLPARLLDVLAQPAPPQPPQAVQRPWLRAAMVAGCLLG
                     AGIAGAFGGWFGHARFGGDWPVAAGPDAAFVERAVYAHAVYVPEVRHPVEVAAAESQH
                     LFGWLSKRMGHTIRAPGLEALGFELLGGRLLPGEEGRPAAQFMYQNADGRRLTLYVNR
                     CDARGETSFRFHHEDSLNAFYWLDGELGYALVGDVSREELSAAAHQVYRQLAL"
BASE COUNT          281 a          721 c          764 g          309 t
ORIGIN      
        1 acgacgggcg gtccgcagac cgcccgccgg cgtagctcgt gaagcaggtc gtgaaaccgg
       61 cttcgtgcgc tgactgcctt actgcgagat cagcgcgtgc tcgtactcgg cgcgccccag
      121 gcagccgcgg cgggcggtgt cggccttcgc gaagttcagg atgcccatgt ccttggcttc
      181 cctcgggtcg atgcagccgt tgccgtcccg gtcggcccac tcgaagctct ggtcgggaac
      241 cgagttcggc acttcgcgct gctcgacctg gcgggtgacg gtgccgtcgg gattcgtgat
      301 ggtcgtgacg atggtctcgg tcttggcctg cgcgaggccg atgccggaaa gcagcgcggc
      361 cgcggcgaac agggcgagca tgcggttcgg gcgattcatg gtgaaggtct ccttctggat
      421 ctggcctgat ctggcctggt ctggatcggc acggaagcgg ccggtgggcg cctgcggcag
      481 caggccatcc gtccccgctg cgggcagggg cggaccgtaa cgcaaggtgg gatggcggat
      541 cgccggcgaa gttcgcggat cccccgggaa gggctgcaac ctttggggtg tagacgtggt
      601 gtacgtgcag ggtagaacac gaactccgga ggtccagacc ttgaaccgtc gcaggaagcc
      661 ccgtccgtga gccgcatgct cgccgacgcc atgaccgccg agattccgcg cctgcgccgc
      721 tacgcgcgcg cgctgaccgg cgacccggag cgtgccgacg atctggtgca ggactgcctg
      781 gagcgcgcct ggacccgtca gcaccactac gacggcagca gtgagctgcg gccgtggctg
      841 ttcacgatcc tgcacaacct gcacgccaac gccgcgcgca gctatcaccg ccggccggcc
      901 ttcgtcgatg tcgacgcgct ggccgaggcc gcaccggtgg ccccgaacca cgacggtgaa
      961 ctggcactgc gcgatctggc acaggccatg gaccggctct ccgacgagca gcgcgcggtg
     1021 ctgctgctga tcggcgtcga ggagctgacc tatccgcagg ccgccgccgt gctcggcgtg
     1081 ccggtcggca ccgtgatgtc gcgcctgtcg cgggcgcgcg agcgcctgcg gcaactgctg
     1141 gccggggccg atgacggccc gcggctgagg agggtcaagt gatgaacggc gagtccggca
     1201 ggcgcatcgc cgaggccgag ctgcacgcct ggctcgacgg tcgcctcgaa cccgaacggc
     1261 gcgcggcggt cgaggactgg ctggccgcca accccgacga ggccgagcgc ctcgcgagct
     1321 atcacacggc cgaggccggc ctgcgggaac tgtacgcacc ggtgctcgaa gaaccgctgc
     1381 cggcacgact gctcgacgtg ctcgcgcagc cggcaccccc gcagcctccg caagccgtgc
     1441 agcggccgtg gctgcgggcg gcgatggtcg ccggctgcct gctgggcgct ggcatcgccg
     1501 gcgcattcgg cggctggttc ggacacgcgc gcttcggcgg ggactggccg gtggcggccg
     1561 gccccgacgc cgccttcgtc gagcgcgcgg tctacgcgca cgccgtctac gtgcccgagg
     1621 tccgccatcc ggtcgaggtc gccgccgccg agagccagca cctgttcggc tggctgtcga
     1681 agcgcatggg ccacacgatc cgcgcgcccg gtctggaggc gctcggcttc gagctgctcg
     1741 gcggccggct gctgccgggc gaggagggcc gtccggccgc gcagttcatg taccagaacg
     1801 ccgatggccg gcggctgacg ctgtacgtga accgctgcga tgcccgcggc gagacctcct
     1861 tccgcttcca ccacgaggac agcctgaatg cgttctactg gctcgacggc gaactcggct
     1921 acgcgctggt cggcgacgtg agccgcgagg aactctcggc cgcggcacat caggtgtacc
     1981 ggcagctggc gctgtgagcg gcggccggcg gcccggggcc tcttgccgga ccctgccgtg
     2041 cctgccgctc agccgggaag gcagggtctt tcttc
//
LOCUS       DAOQJH010000559         2074 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_42945_length_2074_cov_3.267954, whole genome shotgun sequence.
ACCESSION   DAOQJH010000559 DAOQJH010000000
VERSION     DAOQJH010000559.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2074
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42945_length_2074_cov_3.267954"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(56..1003)
                     /locus_tag="PLQ18_11695"
     CDS             complement(56..1003)
                     /locus_tag="PLQ18_11695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014778188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HNE02358.1"
                     /translation="MAGFRPAFGLGGAHRQTLAAALLPAPRPPLARERFELPDGDFVD
                     LDWHRDARGPAVLVLHGLEGDSRSAYARRLLAALARCGRRAGVLHARGRSGEPNRLAR
                     SYTAGDTDDLACVLARLQQRDGRAPDVVGYSLGANRLLKHLGEAAGRAGIRRAVAVSP
                     PFDLGRAADRLDRGLSRLYQWHLLRSLRAGYRVKLAQHPQALPLGADDLDALKTFRSF
                     DDRVTAPLHGYAGVEDYYGRCSCRPFLKDIAVPTLIVHALDDPFLYADAIPTPAELAP
                     AVRLELAAHGGHVGFLQGLSWPAQDWLARRIATFLDASD"
     gene            complement(1015..1212)
                     /locus_tag="PLQ18_11700"
     CDS             complement(1015..1212)
                     /locus_tag="PLQ18_11700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNE02359.1"
                     /translation="MAYHEPVIPRDALYSIDTAARTLGVQRQHIERWLASGILHTWDP
                     SGAWPLRGQDLIDFLRRYGQS"
     gene            1319..>2074
                     /locus_tag="PLQ18_11705"
     CDS             1319..>2074
                     /locus_tag="PLQ18_11705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018415452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNE02360.1"
                     /translation="MDPLNNPRYISRLTRNTLALILAGGSGGRLQELVQWRAKPGVFF
                     GGKFRIIDFPISNCMNSGIRRVCVLTQYKSYSLIRHLINAWGSLKGELGEFVEVLPAS
                     QRYSKQWYAGTADAVYQNLDIIRMHRPSHVLVLAGDHIYKMDYGPMLAYHVEKGADMT
                     VACIETPVAEATAFGVMSVDEHARVTRFTEKPAHPESIPGQPGVALASMGNYVFNTDY
                     LFEQLIRDADDPESAHDFSINVIPRMIRDGHSVY"
BASE COUNT          331 a          709 c          745 g          289 t
ORIGIN      
        1 acggactttg tgacccgccg gtttcgccgg aggttcgcgg caggagcgtt tcgcgtcagt
       61 cgcttgcgtc gagaaaggtc gcgatgcgcc gcgcgagcca gtcctgcgca ggccatgaca
      121 gaccctgcag gaagccgacg tgaccgccgt gtgcggccag ttccagacgc acggccggtg
      181 ccagttccgc gggcgtcggg atcgcatcgg cgtacaggaa gggatcgtcg agggcgtgca
      241 cgatcagcgt cggcaccgcg atgtctttca ggaaaggccg gcagctgcag cggccgtagt
      301 agtcctcgac cccggcatag ccgtgcagcg gcgcggtgac gcgatcgtcg aagctgcgga
      361 aggtcttcag cgcatcgaga tcatccgccc cgagcggcag cgcctgcgga tgctgcgcga
      421 gcttcacgcg atagccggcg cgcaggctgc gcagcagatg ccactggtac agccgcgaca
      481 gcccgcggtc gagccggtcg gcggcgcggc cgagatcgaa cggcggcgac acggcgaccg
      541 cacgccggat gccggcccgg cctgcggctt cgccgaggtg cttgagcagg cggttggccc
      601 cgagcgaata gccgaccacg tccggtgcgc gaccgtcgcg ctgctgcagg cgcgcgagca
      661 cgcaggcgag atcgtcggtg tcgccggccg tgtagctgcg cgccaggcgg ttcggttcgc
      721 cggaacgccc gcgtgcgtgc agcacgccgg cgcgtcgtcc gcagcgcgcg agcgccgcga
      781 gcagccggcg ggcgtaggcg gaacggctgt cgccttcgag gccgtgcagc accagcacgg
      841 ccgggccgcg cgcgtcgcgg tgccagtcga gatcgacgaa atcgccgtcc ggcagttcga
      901 agcgttcgcg ggcgaggggc gggcggggtg ccggcagcag cgcggcggcg agcgtctggc
      961 ggtgcgcgcc gccgagaccg aaggccggac ggaagccggc cacggtgatc cagctcagga
     1021 ctggccgtag cgacgcagga agtcgatgag atcctggccg cgcagcggcc aggcgccgct
     1081 cgggtcccag gtgtgcagga tgccgctcgc gagccagcgc tcgatgtgct ggcgctgcac
     1141 gccgagcgtg cgcgcggcgg tgtcgatcga gtacagcgca tcgcgcggga tgacgggttc
     1201 gtgataagcc atgatcgggc actgactcgg acggctgccg ggcgggcagg ggcgtgatgg
     1261 taagatgccc ggccccgcca gcccaagccc accgcccgcg acgaggttcc ggcccgccat
     1321 ggacccgctg aacaatccgc gttacatcag ccgactgacc cgcaacacgc tcgcgctgat
     1381 cctcgcgggc ggcagcggcg ggcggctgca ggaactcgtg caatggcgcg ccaagcccgg
     1441 cgtgttcttc ggcggcaagt tccggatcat cgacttcccg atctcgaact gcatgaactc
     1501 cggcatccgc cgggtctgcg tgctgacgca gtacaagtcg tactcgctga tccgccacct
     1561 gatcaacgcc tggggctcgc tgaagggcga gctcggcgag ttcgtcgagg tgctgccggc
     1621 ctcgcagcgc tactcgaagc agtggtacgc cggcacggcc gacgccgtgt accagaacct
     1681 cgacatcatc cgcatgcacc gcccgagcca cgtgctcgtg ctcgccggcg atcacatcta
     1741 caagatggac tacggtccga tgctcgcgta ccacgtcgag aagggcgcgg acatgaccgt
     1801 cgcctgcatc gagacgccgg tggccgaggc gacggccttc ggcgtcatgt cggtcgacga
     1861 gcacgcgcgc gtgacgcgct tcaccgagaa gcccgctcat cccgagagca tccccggcca
     1921 gcccggcgtc gcgctggcgt cgatgggcaa ctacgtgttc aacaccgact acctgttcga
     1981 gcagctgatt cgcgacgccg atgacccgga gtccgcgcac gacttcagca tcaacgtcat
     2041 cccgcgcatg atccgcgacg gtcacagcgt ctac
//
LOCUS       DAOQJH010000560         2073 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_42978_length_2073_cov_4.896928, whole genome shotgun sequence.
ACCESSION   DAOQJH010000560 DAOQJH010000000
VERSION     DAOQJH010000560.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2073
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42978_length_2073_cov_4.896928"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..379)
                     /locus_tag="PLQ18_11710"
     CDS             complement(<1..379)
                     /locus_tag="PLQ18_11710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018950042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HNE02361.1"
                     /translation="MSTPERLERIREHEGWLERWRHDSQSCACPMTFSIYLPPQAEAG
                     PVPAVYWLSGLTCSDDNFRVKAGAQRYAAELGLALLIPDTSPRGDEVPDDPGYDLGQA
                     AGFYVEAIRAPWATHYHMHDYVAF"
     gene            complement(442..1551)
                     /locus_tag="PLQ18_11715"
     CDS             complement(442..1551)
                     /locus_tag="PLQ18_11715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018950041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0051903 - S-(hydroxymethyl)glutathione
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0009404 - toxin metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase/class
                     III alcohol dehydrogenase"
                     /protein_id="HNE02362.1"
                     /translation="MKARAAVAWEAQKPLSVTEIEVEGPRAGEVLLRTVATGVCHTDA
                     FTLSGDDPEGVFPCVLGHEGGCIVEECGPDVKGLKPGDHVIPLYIPECGHCEYCASTK
                     TNLCQSIAATVWTGYMPDGTRRFAKDGKPIFHYMGCSTFSEYTVVPEIALAKINPAAP
                     LDKVCLLGCGITTGIGAVLNTAKVEPGSTVAVFGLGGIGLSVIQGAVMAKAGRIIAID
                     TNPAKFDMARALGATDCVNPEALTVSTREAIVEMTHGGVDYSFECIGNVEVMREALEC
                     THMGWGVSTIIGVAGAGREIRTRPFQLVTGRTWKGTAFGGVKGRSQLPGFVDQYLNGT
                     IRIDPMVTHTMGLEDINHAFELMHEGKSIRSVIVF"
     gene            1770..>2073
                     /locus_tag="PLQ18_11720"
     CDS             1770..>2073
                     /locus_tag="PLQ18_11720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011979314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HNE02363.1"
                     /translation="MSPALRRWLPFLAWPRPTRATLAADLRAGLAVGLVLIPQALAYA
                     TLAGMPAQTGLYAALLPAVVGCLWGSSPLLAAGPVALTSLLSFGALQPFAAPGSSGW"
BASE COUNT          283 a          707 c          754 g          329 t
ORIGIN      
        1 caaaggcgac gtagtcgtgc atgtggtagt gcgtggccca cggtgcgcgg atcgcctcga
       61 cgtagaagcc ggcggcctgg ccgaggtcgt agccgggatc atccggcacc tcgtcgccgc
      121 gcgggctggt atccggaatc agcagcgcca ggccgagttc ggcggcgtag cgctgtgcgc
      181 cggccttgac gcggaagttg tcgtcggagc aggtcaggcc cgacagccag tacacggccg
      241 gcaccgggcc ggcctcggcc tgcggcggca ggtagatcga aaacgtcatc ggacaggcgc
      301 agctttgcga gtcatgccgc cagcgttcga gccagccttc atgctcgcgg atgcgttcga
      361 ggcgttcggg ggtcgacatc ggggcggggc ctcgcggcag gtaaggacgg ggaaagccgg
      421 gcgaagcggc ggccggtcca ttcagaacac gatcaccgag cggatgctct tgccttcatg
      481 catcagctcg aaggcatggt tgatgtcttc cagacccatc gtgtgcgtga ccatcgggtc
      541 gatgcggatc gtgccgttca ggtactgatc gacgaagccc ggcagctgcg agcggccctt
      601 gacgccgccg aaggccgtgc ccttccaggt gcggccggtc acgagctgga acggccgggt
      661 gcggatctcg cggccggcac cggcgacgcc gatgatcgtc gacacgcccc agcccatgtg
      721 cgtgcattcc agggcctcgc gcatgacctc gacgttgccg atgcattcga agctgtagtc
      781 gacgccgccg tgcgtcatct cgacgatggc ctcgcgcgtg ctgaccgtga gcgcctccgg
      841 gttcacgcag tcggtggcgc cgagcgcgcg cgccatgtcg aacttggccg ggttggtgtc
      901 gatggcgatg atgcggccgg ccttcgccat caccgcgccc tggatcacgc tcaggccgat
      961 gccgccgagc ccgaacaccg cgaccgtgct gccgggctcg accttcgcgg tgttcagcac
     1021 cgcgccgatg ccggtggtga tgccgcagcc gagcaggcag accttgtcga gcggggcggc
     1081 cgggttgatc ttcgcgagcg cgatctcggg cacgaccgtg tattcgctga aggtcgagca
     1141 gcccatgtaa tggaagatcg gcttgccgtc cttcgcgaag cggcgcgtgc cgtcgggcat
     1201 gtagccggtc cagaccgtcg cggcgatcga ctggcagagg ttggtcttcg tcgaggcgca
     1261 gtactcgcag tgaccgcatt cggggatgta cagcgggatc acgtgatcgc cgggcttcag
     1321 gcccttcacg tccggcccgc actcctcgac gatgcagccg ccctcgtggc cgagcacgca
     1381 cgggaagacg ccctcggggt cgtcgccgga cagcgtgaag gcatcggtgt ggcagacccc
     1441 ggtggcgacc gtgcgcagca gcacttcgcc ggcacgcggc ccctcgactt cgatctcggt
     1501 gaccgagagc ggcttctgcg cttcccaggc aacggcggca cgggctttca tcggcaggct
     1561 cctcgtgtgg ggcgatgccc gcaggcatcg ggcggccgcg gacggctcga accgctacag
     1621 gctagacggc agcatcaggg atgcgcaggc gaccaccggc cccggcacgc cggggaatgg
     1681 ccggtctctg caggactgcg ggcggcgggc gccggttcct gtcaggctgg atacccgtcc
     1741 gccaccgccg ccggaggcct tcgccgatca tgtcccctgc cctgcgccgc tggctgccct
     1801 tcctcgcctg gccgcgtccg acgcgcgcga cactcgctgc cgacctgcgc gccgggctcg
     1861 cggtcgggct ggtgctgatc ccgcaggcgc tcgcctacgc gacgctcgcc ggcatgccgg
     1921 cgcagaccgg gctgtacgcg gcgctgctgc cggcggtggt cggctgcctg tggggctcgt
     1981 cgccgctgct cgccgccggg ccggtggcgc tgaccagcct gctgagcttc ggcgcgctgc
     2041 agccgttcgc ggcgccgggc agcagcggct ggg
//
LOCUS       DAOQJH010000561         2071 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_43068_length_2071_cov_3.161706, whole genome shotgun sequence.
ACCESSION   DAOQJH010000561 DAOQJH010000000
VERSION     DAOQJH010000561.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43068_length_2071_cov_3.161706"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            234..731
                     /locus_tag="PLQ18_11725"
     CDS             234..731
                     /locus_tag="PLQ18_11725"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HNE02364.1"
                     /translation="MQLLLIRHGIAEDTAPSGGGDAERALAAVGARRLRKGANRLRMQ
                     FERLDHVLCSPYRRTRESAAILCAAWGLAAPQTIAGCTPHDAPEDVLPVLAGLDPDAH
                     VALVGHEPHLGCLAGLLLSGSAEPLLRLRKGGAALIVFPGAPAAGTGRLHWALTAGQM
                     RALRD"
     gene            780..>2071
                     /gene="ppx"
                     /locus_tag="PLQ18_11730"
     CDS             780..>2071
                     /gene="ppx"
                     /locus_tag="PLQ18_11730"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012806891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HNE02365.1"
                     /translation="MPVDTFAAIDLGSNSFHMIVARAGDGTLQVVDRLREMVQLAAGL
                     DAAHRLDEATQQRALDCLARFGQRIAQLPPERVRIVGTNTLRQARNGAAFLERATQVL
                     GHRVEIISGREEARLIYLGVAHSTAGSGRRLVVDIGGGSTELIVGEQFEPQQLESLYM
                     GCVSLTRRFFDAERIRERALHEADLSVRMELEPVEAGFRDAAWDEAIGASGTIKAIRD
                     VLVALGWSSGDIPREALRRLRTDLIDAAPAVFGERFGLARERAAVFAGGLAVLHGIFE
                     TLGIECMKVSDGALREGLIFDLLGRVQHEDVRDRSVRALAARFGCDEAQGGRVAATAR
                     ELLAQAAPGWGIADEEAARYLDWSARLHELGLAIAHNQYHKHGGYVLANADLPGFSKT
                     EQALLAALVRGHRRKLPLDTFTALPAGQAERGLRLCLLL"
BASE COUNT          275 a          794 c          716 g          286 t
ORIGIN      
        1 gtcggcgccg gcagccgctt cgcgtgcgat ttcccggccg aactggcctg cccgcgcacc
       61 agcggcggcc gcgccgcctg aggccggtca cgccggaacg tttttttctt tcagcgtctt
      121 tcagcccctc gcccctcgcg ggcaagggac tggcaagagg ggagaaaagt catgcccgta
      181 ccatcggccc gacttccccg ccgcgaaccg ccttcattag ggaggaatcc gccatgcaac
      241 tgctgctgat ccgccacggc atcgccgagg acaccgcccc gtccgggggc ggcgatgccg
      301 agcgtgcgct ggccgccgtc ggcgcccggc gcctgcgcaa gggcgccaac cgcctgcgca
      361 tgcagttcga acggctggac cacgtgctct gcagcccgta ccggcggacg cgcgagagcg
      421 ccgcgatcct gtgcgcggcc tgggggctgg ccgcaccgca gaccattgcc ggctgcaccc
      481 cgcacgacgc gcccgaggac gtgctgccgg tgctcgccgg actcgatccg gacgcgcacg
      541 tggcgctggt cggccacgaa ccgcacctcg gctgcctcgc cgggctgctg ctcagcggca
      601 gcgccgaacc gctgctgcgg ttgcgcaagg gcggcgcggc gctgatcgtg tttcccggtg
      661 cgccggctgc cggcaccggc cggctgcact gggccctgac cgccggccag atgcgcgcgc
      721 tgcgcgactg aagcccgcct tacactgtcc gcctccccgc ccgccgccgg atcgtccgca
      781 tgcccgtcga caccttcgcc gcgatcgacc tcggctccaa cagcttccac atgatcgtcg
      841 cccgcgccgg cgacggcacg ctgcaggtcg tcgatcgcct gcgcgagatg gtccagctcg
      901 ccgccggcct cgacgccgcg caccgcctcg acgaggccac gcagcagcgc gcgctcgact
      961 gtctcgcccg cttcggccag cgcatcgcgc agctgccgcc cgagcgcgtg cgcatcgtcg
     1021 gcaccaacac gctgcgtcag gcgcgcaacg gcgcggcctt tctggagcgc gccacgcagg
     1081 tgctcggcca ccgcgtcgag atcatcagcg gtcgcgagga agcgcggctg atctacctcg
     1141 gcgtcgcgca ctcgaccgcc ggcagcggcc ggcggctggt ggtcgacatc ggcggcggca
     1201 gcaccgagct gatcgtcggc gaacagttcg agccgcagca gctcgaaagc ctgtacatgg
     1261 gctgcgtgag cctgacgcgc cgcttcttcg atgccgaacg catccgcgag cgcgcgctgc
     1321 acgaagccga cctcagcgtg cgcatggaac tggaaccggt cgaggcgggc tttcgcgacg
     1381 ccgcctggga cgaggccatc ggcgcctccg gcacgatcaa ggccatccgc gacgtgctgg
     1441 tcgcgctcgg ctggagcagc ggcgacatcc cgcgcgaggc gctgcgtcgc ctgcgcaccg
     1501 atctgatcga cgccgcgccg gccgtgttcg gcgagcgctt cgggctggca cgcgaacgcg
     1561 ccgcggtgtt cgccggcggg ctggccgtgc tccacggcat cttcgagacg ctggggatcg
     1621 aatgcatgaa ggtatcggat ggcgccttgc gcgagggcct gatcttcgac ctgctcgggc
     1681 gtgtgcagca cgaggacgtg cgtgaccgca gcgtgcgcgc cctcgccgcc cgcttcggct
     1741 gcgacgaggc ccagggcggc cgcgtcgctg ccaccgcccg cgaactgctc gcgcaggccg
     1801 cgcccggctg gggcatcgcc gatgaggaag cggcacgcta tctggactgg tccgcgcgcc
     1861 tgcacgaact cgggctggcg atcgcgcaca accagtacca caagcacggc ggctacgtgc
     1921 tcgccaacgc cgacctgccg ggcttctcga agaccgagca ggccctgctc gcggcgctgg
     1981 tgcgcggcca ccgccgcaag ctgccgctcg acaccttcac ggcgctgccg gccggtcagg
     2041 ccgaacgcgg cctgcgcctc tgcctgctgc t
//
LOCUS       DAOQJH010000562         2070 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_43101_length_2070_cov_4.006948, whole genome shotgun sequence.
ACCESSION   DAOQJH010000562 DAOQJH010000000
VERSION     DAOQJH010000562.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2070
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43101_length_2070_cov_4.006948"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(153..1091)
                     /locus_tag="PLQ18_11735"
     CDS             complement(153..1091)
                     /locus_tag="PLQ18_11735"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HNE02366.1"
                     /translation="MEQRLKQRLLGTAVLVALAVIIVPEVVKTSGPVTAPAVATSIPP
                     RPESPQPGGVTVALPPAILPQAVPPEAPAPSGQAPASTIAQSPVIEPLRTPAALPSAS
                     AGGDGPQGMRTVDLGAGAPRVAPANPAPAAPPARPAAPKPAPVVASAPASKPAQTPAA
                     GATPSKPAPETARATTDAPKPKPEVVAKAAPATVTPAATARPVSLPKIELTSRDTSRA
                     SASAPAAEPWRPQASNGSWAVQVGSFGVEENANLLRDRLRSQRFPAVVERIRGGSGQA
                     MYRVRVVGQDSRPASEAIASRLRSAGVAANIVEPGE"
     gene            complement(1160..>2070)
                     /locus_tag="PLQ18_11740"
     CDS             complement(1160..>2070)
                     /locus_tag="PLQ18_11740"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HNE02367.1"
                     /translation="TYFEFGTLAALWLARAFDPEVLVLEVGLGGRLDAVNIIDADVAI
                     VTTIGIDHTEFLGPDRESIGCEKAGIYRPGRPAICVDPSPPASLLAHQQAIGARPYRF
                     GHEFGVREARGGWSWWGPGGGLDLPWPSLAGPHQLANAAGAVAALRLLPSIEVSGAAI
                     ASGIAAARLAGRFQIESGPVEWVFDVTHNPHGAAVLAETLQWRPVAGQTWLLLAMLAD
                     KDAAGVIAALMPVVDRWCAAGLDGARGRPVESMLALLPAGTMACKDVTAACRQLESAA
                     QAGDRVVVCGSFLTVAEAMRARHITC"
BASE COUNT          341 a          697 c          704 g          328 t
ORIGIN      
        1 aggcgcgcat tctacacgcc ttcttcaatt ccgcaagcag tgttttcacc gcttttcatc
       61 gacatcaaac aagtcctttg atgcgacgag acggacgatt atatgggatc acagaagctt
      121 gtcaagagcc gaacatgcac tgaaccccga tctcactctc ccggctcgac gatgttggcc
      181 gccacgcctg ccgagcgcag acgcgacgcg atcgcctcgc tggcgggtct ggaatcctgt
      241 ccgacgacgc ggacccgata cattgcctgg ccgctgcctc cacggatgcg ttcgacgacg
      301 gccgggaagc gctgggagcg caggcggtcc cgcagcagat tggcgttttc ctcgactccg
      361 aagctgccga cctgcaccgc ccaggagcca ttgctggcct gaggcctcca gggttcggcc
      421 gccggtgcgg aagcactggc tcttgaagtg tcgcggctgg tcagctcgat cttcggcagc
      481 gaaaccggtc tggctgtcgc cgccggggtc acggtagcag gagctgcctt ggccacgacc
      541 tcaggcttgg gcttcggcgc atcggtcgtt gctctggccg tttccggagc cggcttcgaa
      601 ggagtggccc cggcagcagg agtctgagcc ggtttcgaag cgggtgcgct tgcaacgacc
      661 ggtgccggct tcggggctgc aggtctggct ggcggtgccg ccggtgccgg gttggccgga
      721 gccacgcgcg gcgcgccagc gcccagatcg accgtccgca tgccttgcgg accatcaccg
      781 cccgcgcttg ccgatggcag ggctgcgggc gttcgcagcg gttcaataac cggagactgg
      841 gcgatcgtcg atgccggtgc ctgcccggac ggagccggtg cttcgggcgg cacggcctgc
      901 ggcaggatcg ccggcggcag cgcgacggtg accccgccgg gctgcggcga ttccggtctg
      961 ggcgggatgc tggttgcgac ggccggcgcg gtgaccggac cggaagtctt gaccacttcc
     1021 gggacgatga tcacggccag tgcgaccagt acggcggtgc cgagcagacg ctgcttcaga
     1081 cgctgttcca cgacacgact ccgggcatga actttggctg ggggcgatac gagtcagggt
     1141 ttcaggacat tcaagacatt cagcacgtaa tgtgacgcgc ccgcatggct tcggcaacgg
     1201 tcaggaacga tccgcagacg accacacgat cgccggcctg cgccgccgat tccagttgcc
     1261 ggcaggcagc cgtcacatcc ttgcaggcca tggtacctgc tggcagcaag gccagcatcg
     1321 attcgaccgg acgcccgcgt gcaccgtcga gcccggccgc acaccagcgg tccacgaccg
     1381 gcatcagcgc tgcgatgacg ccggcagcgt ccttgtcggc aagcatggcc agcagcagcc
     1441 aggtctgacc ggccacgggc cgccactgca gcgtttcggc cagtaccgca gcaccgtgcg
     1501 gattatgggt gacatcgaac acccactcca cgggtcccga ttcaatctgg aaccgtccgg
     1561 cgagtcgggc agcggcaatc ccgctggcga tggcagcccc ggaaacctcg atcgacggca
     1621 gcagacgcag ggccgcaacc gcccccgccg cgttcgccag ttgatgcggt cctgcaagag
     1681 agggccaggg caggtcaagc ccgccgccgg gcccccacca gctccagccg ccgcgcgcct
     1741 cccggacccc gaactcgtga ccgaagcggt acggccgggc gccgatcgcc tgctgatgcg
     1801 cgagcaggct ggccggtggc gacggatcga cgcagatcgc cggtcgtccg ggacggtaga
     1861 tgccggcctt ctcgcagccg atcgattcgc ggtcggggcc gaggaattcg gtgtggtcga
     1921 tgccgatcgt ggtgacgatg gccacgtcgg cgtcgatgat gttgacggca tcgagccgtc
     1981 cgccgagccc gacctcgagc acgagcactt cgggatcgaa ggcccgtgcc agccagagcg
     2041 ccgccagcgt gccgaattcg aagtaggtca
//
LOCUS       DAOQJH010000563         2070 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_43110_length_2070_cov_3.246650, whole genome shotgun sequence.
ACCESSION   DAOQJH010000563 DAOQJH010000000
VERSION     DAOQJH010000563.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2070
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43110_length_2070_cov_3.246650"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..718)
                     /locus_tag="PLQ18_11745"
     CDS             complement(<1..718)
                     /locus_tag="PLQ18_11745"
                     /inference="COORDINATES: protein motif:HMM:NF001464.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HNE02368.1"
                     /translation="MWFKSLMLFRFTEPCTLDPDTLAEALAHAAFRPCGSQDLVTCGW
                     VPPLGGEDGALVHAAGGFWLVCLQSEDKLLPASVIRAALDERVAEIEDREARKVRRKE
                     KETLRDEVLLDLLPRAFTRRKRLYAYLDPRGGWLVVNGTPGKAVDDFTSLLRKAAGSL
                     PIAPLRTASVPASVLTGWLGGAPADWQVGDECELRDAGEEGGIVRCKGQDLGGDEIRT
                     HLAAGKQVTRLAVEWQERLSC"
     gene            943..1311
                     /locus_tag="PLQ18_11750"
     CDS             943..1311
                     /locus_tag="PLQ18_11750"
                     /inference="COORDINATES: protein motif:HMM:TIGR02809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HNE02369.1"
                     /translation="MADNFEKFFEQTKTLGAPVVKANKLAVASLEKLVALQLSTLQSY
                     TDLTIARLKAAAEVTDLKSLQAFAADQVELAKSLGQKLMDDAKAYADLGAGVKAEFDK
                     LAEEAAAELPKSVQSFSKAA"
     gene            complement(1429..>2070)
                     /locus_tag="PLQ18_11755"
     CDS             complement(1429..>2070)
                     /locus_tag="PLQ18_11755"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNE02370.1"
                     /translation="PLNAIIGFTGILLMKLSGPLSAEQEQQLRTVRSAADHLLALISD
                     LLDLARIESGAAELSREPVDLAEVLAETQRTLLPLAGERGLVLALQVPDGDWTIPCNR
                     RAFGQIVLNLAGNAIKFTPAGRIDVRLARIADGSGERIQLSVSDTGPGLDPEAQARLF
                     RPFEQLGTPARQEGTGLGLYLSQKLAHQLGGHISCRSKPGEGCTFTLVLPTGE"
BASE COUNT          325 a          723 c          710 g          312 t
ORIGIN      
        1 cgcaggacag ccgctcctgc cattcgacgg ccaggcgcgt gacctgcttg ccggcggcaa
       61 gatgcgtgcg gatctcgtca ccgccgaggt cctggccctt gcagcgcacg atgccgccct
      121 cctcgccggc atcgcgcagc tcgcattcgt cgccgacctg ccagtcggcc ggcgcgcccc
      181 cgagccagcc ggtcagcacg ctcgccggca ccgacgcggt gcgcagcggc gcgatcggca
      241 ggctgccggc ggccttgcgc agcagcgagg tgaagtcatc gaccgccttg cccggcgtgc
      301 cgttgaccac cagccagccg ccgcgcggat cgaggtaggc gtacaggcgc ttgcgccgcg
      361 tgaacgcgcg cggcagcagg tcgagcagga cctcgtcgcg cagcgtttcc ttttccttgc
      421 gccggacctt gcgtgcctcg cggtcctcga tctcggcgac gcgctcatcg agcgcagcac
      481 ggatcacgga ggccggcagg agtttgtcct cgctctgcag gcacacgagc cagaagccgc
      541 cggccgcgtg caccagcgcg ccgtcctcgc cgccgagcgg cggcacccag ccgcaggtga
      601 cgaggtcctg cgaaccgcag ggccggaagg ccgcgtgcgc gagcgcctcg gcgagcgtgt
      661 cgggatcgag cgtgcagggt tcggtgaagc gaaacagcat caggctcttg aaccacatgc
      721 gggacggcat ccgggtggcg gcagggggcg cgcatcataa gcaggcgggc tgccggcgtc
      781 atcgccggct catttatccg agccggaaca gcgcagtacg gtcagccgaa taaccttaat
      841 gaaatcttgg tcatctatgt tgcacggcaa catgaatgtc gatatgctgc ttcgcaacat
      901 gatcgttcaa cccaacgctt gacccctttg gaggaagtag ccatggccga caacttcgag
      961 aagttcttcg agcagaccaa gaccctcggc gccccggtcg tcaaggccaa caagctggcc
     1021 gtcgccagcc tggagaagct ggtcgcgctg cagctgagca cgctgcagag ctacaccgac
     1081 ctgaccatcg cccgcctgaa ggccgcggcc gaagtcaccg acctcaagag cctgcaggcc
     1141 tttgccgcgg atcaggtcga gctcgccaag agcctgggcc agaagctgat ggacgacgcc
     1201 aaggcctatg ccgacctcgg cgccggcgtc aaggccgagt tcgacaagct ggccgaagaa
     1261 gccgctgccg agctgccgaa gtcggtgcag tcgttctcga aggctgcctg atgccgccgc
     1321 ggcgctgaac gcgccgcccg gtctgccgat ccggaccgcc gccgcccgca ggggccgcgg
     1381 cggttctgct ttttcgcggt accgtccttt tcacccgtga ccggcttttc actcgccggt
     1441 cggcagcacc agcgtgaacg tgcagccctc ccccggcttg ctgcggcagc tgatgtggcc
     1501 gccgagctgg tgtgcgagct tctggctcag gtacaggccg aggccggtgc cctcctgacg
     1561 ggccggcgtg ccgagctgtt cgaagggccg gaacagccgg gcctgcgcct ccgggtcgag
     1621 gcccgggccg gtgtcgctga cgctgagctg gatgcgctcg ccgctgccat cggcgatgcg
     1681 tgccagccgc acgtcgatgc gcccggccgg cgtgaacttg atggcgttgc cggcgaggtt
     1741 cagcacgatc tggccgaagg cgcgacggtt gcacgggatc gtccagtcgc cgtccggcac
     1801 ctgcagcgcc agcaccagac cgcgctcgcc ggcgagcggc agcagcgtgc gctgcgtctc
     1861 ggcgagcacc tcggccagat cgaccggctc gcgcgacagc tcggcggcac cggattcgat
     1921 gcgggcgaga tcgagcaggt cgctgatcag cgccagcagg tgatcggcgg cgctgcgcac
     1981 cgtgcgcagc tgctgctcct gctcggcact gagcggcccg gacagcttca tcagcaggat
     2041 gccggtgaaa ccgatgatcg cgttcagcgg
//
LOCUS       DAOQJH010000564         2068 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_43177_length_2068_cov_3.759563, whole genome shotgun sequence.
ACCESSION   DAOQJH010000564 DAOQJH010000000
VERSION     DAOQJH010000564.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2068
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43177_length_2068_cov_3.759563"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2057
                     /locus_tag="PLQ18_11760"
     CDS             <1..2057
                     /locus_tag="PLQ18_11760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005673694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="restriction endonuclease subunit R"
                     /protein_id="HNE02371.1"
                     /translation="VDEAHNSRTELAFDMLARFRPGGVMELTATPDLERTPTNVLHSV
                     SAAELKAEEMIKLPIVLETEPDWQQCLADAIGRRDALHKLADEERRTGAAYLRPLVLI
                     QAEARRAGVETLDAGRVRHELITHHGIPAGEIVIATGEEKGLEQIDTDYRLGIADPSC
                     PVKFVITQKALAEGWDCPFAYILVSMASLSSATAVEQLLGRVLRQPGASHRQARALNQ
                     SYAFVVSRNFAETAGALRDRLIAGAGFERHEVTEFVTAARPEQARLDLEGHTGRVFYQ
                     PVSIQLSEKPDLRSVPKPVRDKVSWDGKLNTLTIKTPLTEDDTEVLKTSVTSESAATA
                     IIEAAGISRTTAIEFFQTPAELGERFRVPQLALRVQGELQLFDDPEVLDYPWDLSTYD
                     AAPTADDLRALGAGLKVSEGGEIDVDGESGKLISRFLPDLQRDLGLVYQPEHWDELRL
                     ATWLCRNLPEPSLTHAGKQAFVAGWLTELLRREGFTLARANLQKFLIRTLLEARIREL
                     RKQAVSLAFQQTLFGDDAASRVAVTDQYSFEFHALAYAPPRDYDGRFGHFEFRAHFYG
                     RIGDFDSREEFECACWLDIQAQRGRIRFWVRNLVRREGCSFFLQKADGRFYPDFLCQL
                     PDMTDRPGPVLAIEYKGADRWTGAEDDRLIGGLWANLSDERCRFVMVTDKQWDWIEEK
                     MD"
BASE COUNT          398 a          640 c          667 g          363 t
ORIGIN      
        1 tggtggacga ggcgcacaac agccgcaccg aactggcctt cgacatgctg gcgcgattcc
       61 gccccggcgg cgtcatggaa ctgaccgcca cgcccgatct ggaacgcacg ccgaccaatg
      121 tgctgcacag cgtttccgcc gccgagctga aggcggaaga aatgatcaag ctgccgatcg
      181 tgctggaaac cgaaccggac tggcagcaat gtctggccga tgccatcggt cggcgtgatg
      241 ccctgcacaa actggccgac gaggagcgcc ggacgggcgc cgcatatctg cgcccgctgg
      301 tgctgattca ggccgaagcg cgccgcgccg gcgtggagac actcgatgcc gggcgggtgc
      361 gccacgaact gatcacccac cacgggattc cggccgggga gatcgtgatt gcgaccggcg
      421 aggaaaaagg actggaacag atcgacaccg actacaggct gggcattgcc gatccgtcct
      481 gtccggtgaa attcgtcatt acccagaagg cactggccga gggctgggac tgtcccttcg
      541 cctatatcct ggtgagcatg gcctcgctat cgtccgccac ggcagtggag caattgctgg
      601 gccgggtcct gcgacaaccc ggtgccagcc atcggcaggc cagagccctg aatcagtcct
      661 atgccttcgt ggtatcgcgc aacttcgccg agacggcagg cgcgctgcgg gatcgtctga
      721 ttgcaggcgc cggctttgaa cggcacgaag taacggagtt cgtcaccgct gcccggcccg
      781 aacaggcacg cctggacctg gaaggacaca ccgggcgcgt attttaccag ccggtgtcga
      841 tccagctttc cgaaaagccc gatctcagga gcgtgccgaa accggtgcgc gacaaggtga
      901 gctgggacgg caagctcaat accctgacca tcaagacacc gctgaccgaa gacgatacgg
      961 aagttctcaa gacgtcggtc accagcgagt ccgcagcaac ggccatcatc gaggctgccg
     1021 ggatcagccg caccacggcc atcgaattct tccagacgcc ggccgaactc ggtgagcgtt
     1081 ttcgcgtacc gcagcttgcc ttgcgcgtgc agggcgaact gcaactgttt gacgacccgg
     1141 aggtgctgga ttacccgtgg gacttgtcga cttacgacgc cgcaccgacc gcggacgatc
     1201 tgagggcgct gggcgcgggc ctgaaagtat ccgaaggcgg cgagattgat gtcgatggcg
     1261 aaagcggcaa gctcatctcg cgcttcctgc ccgatctgca acgcgatctg gggctggtct
     1321 accagcccga acactgggac gaattgcgtc tcgccacctg gttatgccgc aatctgccgg
     1381 agccctccct cacccacgcc ggcaaacagg ctttcgtggc cggctggctg accgaattat
     1441 tgcgccggga gggcttcaca ctggcgcggg ccaacctgca gaagtttttg atccgcaccc
     1501 tgctggaggc gcgaatccgt gagctgcgca agcaggccgt cagcctggca ttccagcaga
     1561 cgctgttcgg cgatgacgcg gcctcgcggg tcgcagtgac cgatcagtac agctttgaat
     1621 ttcacgcgct ggcctatgcg ccccctcgcg attacgacgg tcgtttcgga catttcgaat
     1681 tccgtgcgca cttttacgga cgcatcggtg acttcgacag cagggaggaa ttcgaatgtg
     1741 cctgctggct ggacattcag gcgcagcggg gacgcatccg gttctgggtc cgcaacctcg
     1801 tgcggcgcga aggctgctcg ttcttcctgc agaaggccga tggccgcttt tatccggatt
     1861 ttctatgtca gttgcccgac atgacagacc ggcccggacc ggttctggcc atcgaataca
     1921 agggcgcgga ccgctggact ggcgcggagg atgaccggct gatcggcggc ctgtgggcga
     1981 acctgtccga tgagcgatgc cggttcgtga tggtcacgga caagcaatgg gattggatcg
     2041 aggaaaagat ggactgagcg gtgcccgg
//
LOCUS       DAOQJH010000565         2067 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_43218_length_2067_cov_3.618787, whole genome shotgun sequence.
ACCESSION   DAOQJH010000565 DAOQJH010000000
VERSION     DAOQJH010000565.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2067
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43218_length_2067_cov_3.618787"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            144..569
                     /locus_tag="PLQ18_11765"
     CDS             144..569
                     /locus_tag="PLQ18_11765"
                     /inference="COORDINATES: protein motif:HMM:TIGR00305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HNE02372.1"
                     /translation="MRAVLDTNVVASAMLWGGQPRLLLDAARAGSLMLCTSAALLAEL
                     ADILSRRKFERKLAAACRSPDQLVAGYAALATAYRPAPVPRIAPDPDDDVVIGTALAA
                     RAPLIVTGDRALLSVGEYADIKLLTVSAALVLLSSHLPG"
     gene            complement(584..1789)
                     /locus_tag="PLQ18_11770"
     CDS             complement(584..1789)
                     /locus_tag="PLQ18_11770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012163712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNE02373.1"
                     /translation="MSLTDLDPELDPPAEDLDPAETRRLRRWRLVLGAPAEHPDCALD
                     AAGLALDSALDALYGEQERGAGLGGSAPRVARWLGDIREHFPVPIVRVLQRDAMERLG
                     LKRLLLEPELMQGLEPDVHLAATLVSLAHVMPAKAKDTARIVVRRVVEDLERRLSEPM
                     RAAVLGSLDRSVRNHRPRHSEIDWPRTIRANLRHWQPELRTVIPERLLGHGRRRSGLR
                     DIVVAIDQSGSMAASVVYASLFAAVMASLRAVNTRLVAFDTNIVDLSDQLADPVDVLF
                     GVQLGGGTDINRAVAYCEKLIERPEDTIFVLISDLIEGGVREQLLSRCARLHAAGVTC
                     VALLALSDEGRPCFDAEIAAALSALGIPAFACTPELFPGLMATAIEKRDVGQWAAQQG
                     LVTTRAAQG"
     gene            complement(1812..>2067)
                     /locus_tag="PLQ18_11775"
     CDS             complement(1812..>2067)
                     /locus_tag="PLQ18_11775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019634245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5682 family protein"
                     /protein_id="HNE02374.1"
                     /translation="ARVLTHDDTLWGLLDDWLAALPEPAFVAVLPLLRRAFAGFTSAE
                     RQRLGSRSRNAAREAATSGTDPVRAGMMVPVLVELLGGGT"
BASE COUNT          307 a          734 c          718 g          308 t
ORIGIN      
        1 acctgttcgc ggcaatggac cgcatggctg ccgtcgatga gcccgcgccg atgacggcgg
       61 aagaactggc tgatgaaatc agaaagttac gattggccag accgggcaag gcggatcagg
      121 actgagttcg cccgggcctc tgcatgcggg ctgttctcga caccaatgtc gtcgcttcgg
      181 caatgctatg gggcgggcag ccccgccttc tgctggatgc ggcgcgagca ggaagcttga
      241 tgctttgcac gagtgccgcc ctgctcgccg aactggccga catcctcagc cggcgcaaat
      301 tcgaacgaaa acttgccgct gcctgtcgct ctcccgatca gctcgtagcc ggctatgcag
      361 cgttggctac cgcctatcgt cctgctccgg ttccgagaat tgcacccgat cccgacgatg
      421 acgtggtgat tggcaccgca cttgccgctc gcgcgccgtt gatcgtgacc ggtgaccgag
      481 ccttgctgtc cgtcggcgaa tacgccgata tcaaactgct gacggtctcc gctgcgctcg
      541 tgctgctcag cagccacctg cccggctgaa ccgtgcgctg gtctcacccc tgcgccgccc
      601 tcgtcgtcac cagcccctgc tgcgccgccc actgccccac gtcgcgcttc tcgatcgccg
      661 tcgccatcag gccggggaac agttccggcg tgcaggcgaa ggccgggatg ccgagggcgg
      721 acagggcggc ggcgatttcg gcgtcgaagc acggacggcc ctcgtcggag agcgcgagca
      781 gcgccacgca ggtgacgccg gccgcgtgca ggcgggcgca gcgcgacagc agctgctcgc
      841 gcacgccgcc ttcgatcagg tcgctgatca gcacgaagat cgtgtcctcg ggccgctcga
      901 tcagcttctc gcagtaggcg acggcgcggt tgatgtcggt gccgccgccg agctgcacgc
      961 cgaacagcac atcgaccgga tcggcgagct ggtcgctcag atcgacgatg ttcgtgtcga
     1021 aggccaccag ccgcgtgttc acggcgcgca gcgaggccat caccgccgcg aacaggctgg
     1081 cgtagaccac cgaggccgcc atcgaaccgc tctggtcgat cgccacgacg atgtcgcgca
     1141 ggcccgagcg gcggcggccg tggccgagca ggcgttcggg gatcaccgtg cgcagctccg
     1201 gctgccagtg gcgcaggttc gcgcggatcg tgcgcggcca gtcgatctcg ctgtggcgcg
     1261 gacggtggtt gcgcacgctg cgatcgaggc tgccgagcac cgccgcgcgc atcggctccg
     1321 acagccggcg ttcgaggtcc tcgaccacgc ggcgcacgac gatgcgcgcc gtgtccttgg
     1381 ccttcgccgg catcacgtgc gcgagcgaca ccagcgtcgc cgccagatgc acgtccggct
     1441 ccagcccctg catcagctcc ggttcgagca gcaggcgctt gaggccgagc cgctccatcg
     1501 catcacgctg cagcacgcgc acgatcggca ccgggaagtg ctcgcggatg tcgccgagcc
     1561 agcgcgccac gcgcggcgcc gatcccccga gcccggcgcc gcgctcctgc tcgccgtaca
     1621 gcgcatcgag cgccgaatcg agcgcgagcc ccgcggcatc gagcgcgcag tccgggtgct
     1681 cggccggcgc gccgagcacc agccgccagc ggcgcagccg gcgggtttcg gcggggtcga
     1741 ggtcttcggc cggcgggtcg agttcgggat cgaggtcggt gaggctcatg cgatgcggcc
     1801 ctgatacagc gtcatgttcc gccccccagc agttccacca gcaccggcac catcatcccg
     1861 gcgcggaccg gatcggtgcc ggaagttgcc gcctcgcgtg cggcgttgcg gctgcggctg
     1921 ccgaggcgct ggcgttcggc ggacgtgaag ccggcgaagg cgcggcgcag cagcggcagc
     1981 acggcgacga aggccggttc cggcagcgcg gcgagccagt catcgagcag gccccacagc
     2041 gtgtcgtcgt gggtcagcac gcgcgcg
//
LOCUS       DAOQJH010000566         2065 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_43295_length_2065_cov_3.814428, whole genome shotgun sequence.
ACCESSION   DAOQJH010000566 DAOQJH010000000
VERSION     DAOQJH010000566.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43295_length_2065_cov_3.814428"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..262)
                     /locus_tag="PLQ18_11780"
     CDS             complement(<1..262)
                     /locus_tag="PLQ18_11780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013717870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HNE02375.1"
                     /translation="MPAEAPHDHSPSAAIAAAEQLCATRGQNLTELRRSVLRIICAAE
                     RPIGAYAILDQLRSEGRSGAPPTVYRTLDFLLEQGLVHRLASL"
     gene            355..1326
                     /locus_tag="PLQ18_11785"
     CDS             355..1326
                     /locus_tag="PLQ18_11785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HNE02376.1"
                     /translation="MHRTVKSLAAAVLLALATGSAVAAPAVVVTIKPVHALVAAVMAG
                     TGAEPKLLVSGRASEHDFALKPSDARAIAKADVVFRVSDQLEGFMRRPLAAAPRVLDV
                     ELIDTPGLVKLAPREGGVWEAHVHGDHDGHDDEGHDHEGHDHEGADHEGGVEPHIWLD
                     PRNARVLGARIAEVLVGLDAANAASYRANAAQLDAGLAALDAGLAQQLAPLAGKPFIV
                     FHDAYQYFEHRYGLTAVGSITVSPERKPSAQRIQAVRDRIRETGARCVFAEPQFDSAL
                     VATLTEGTGARAGSLDPTGADGVPAGPDAYAQVLRRLAENLGACLGG"
     gene            1492..>2065
                     /locus_tag="PLQ18_11790"
     CDS             1492..>2065
                     /locus_tag="PLQ18_11790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02377.1"
                     /translation="MNAAQPLHFPDRPLRVNPPGTGAAAGFGTLLWALLICAGIGWLV
                     LPPVRDLIDDYSLSGSLQPVAHSSVEGRCSYWAGLIVRCDATLGAPAPGGGRYSRKVD
                     YFFVEPLHTGDWTVTVVADPARPALLTTDLALAKLANRSWTLGILGPFFLFGALAALF
                     GARGTLGDRRALKQALSDRVLRPVPLRMEHY"
BASE COUNT          256 a          785 c          721 g          303 t
ORIGIN      
        1 tcagcgaggc gagccggtgc accagccctt gttcgagcag gaagtcgagc gtgcggtaga
       61 cggtcggcgg cgcgccgctg cggccttcgc tgcgcagctg gtcgaggatc gcgtaggcgc
      121 cgatcggccg ttcggccgca cagatgatgc gcagcacgct gcggcgcagc tcggtgaggt
      181 tctggccgcg cgtcgcgcac agctgctcgg ccgcggcgat cgcggcactc ggggaatggt
      241 cgtgcggggc ttcggcgggc atgcgggcga tccgggcgcg ggtggcggca ggggatgtta
      301 tacagtaacg taactgtata acgtctccat cggggagccg ggagcatccg cgccatgcat
      361 cgcaccgtca agtccctcgc cgccgccgtc ctgctcgcgc tcgccaccgg cagcgcggtc
      421 gccgcgccgg ccgtcgtcgt cacgatcaag cccgtgcacg cgctggtcgc cgccgtcatg
      481 gccggcaccg gtgccgagcc gaagctgctg gtcagcggcc gggcctccga gcacgacttc
      541 gcgctgaaac cctcggatgc ccgcgcgatc gcgaaggccg acgtggtgtt ccgcgtctcg
      601 gaccagctcg aaggcttcat gcgccggccg ctcgccgcgg cgccgcgcgt gctcgatgtc
      661 gagctgatcg acacgcccgg cctcgtgaag ctcgcgccgc gcgagggcgg cgtctgggag
      721 gcgcatgtgc atggcgatca cgacgggcat gacgacgagg gccacgacca cgaaggccat
      781 gaccacgagg gcgccgacca cgagggcggc gtcgaaccgc acatctggct cgatccgcgc
      841 aacgcccgcg tgctcggcgc gcgcatcgcc gaggtgctcg tcggcctcga tgccgccaac
      901 gccgcgagct accgagccaa cgccgcgcag ctcgatgccg gactcgctgc cctcgatgcc
      961 ggcctcgcgc agcagctcgc cccgctcgcc ggcaagccct tcatcgtgtt ccacgacgcc
     1021 taccagtact tcgagcaccg ctacggcctc accgcggtcg gctcgatcac ggtcagcccc
     1081 gaacgcaagc cctcggcgca gcgcatccag gccgtgcgcg acaggatccg cgagaccggt
     1141 gcccgctgcg tgttcgccga accgcagttc gattcggcgc tggtggcgac gctgaccgag
     1201 ggcaccggtg cgcgcgccgg ctcgctcgat ccgaccggtg ccgacggcgt gccggccggg
     1261 ccggatgcgt atgcgcaggt gctgcgccgg ctcgccgaaa acctcggcgc ctgcctcggc
     1321 ggctgaaacc cggccggccc tcgcggcgcg ccggccggtc atcgctccgc ctgccccggt
     1381 tccggccgcg cgctcctgcg cttccctgcc atcccggccg cgaccgcgcg ccgctgccgg
     1441 cgccacctat gctcgcggct ctccccccgt cgagcgcgag gtgccgccgg catgaacgct
     1501 gcgcagccgc tgcattttcc cgatcgtccc ctgcgcgtga acccgcccgg caccggtgct
     1561 gcggccggtt tcggcacgct gctctgggcg ctgctgatct gtgccggcat cggctggctg
     1621 gtgctgccgc cggtgcgcga cctgatcgac gactattcgc tcagcggctc gctgcagccg
     1681 gtcgcgcaca gcagcgtcga aggccgctgc tcctactggg ccggcctgat cgtgcgctgc
     1741 gacgccacgc tcggcgcgcc ggctcccggc ggcgggcgct acagccgcaa ggtcgattac
     1801 ttcttcgtcg aaccgctgca caccggcgac tggaccgtca ccgtcgtcgc cgatccggcc
     1861 cgcccggcgc tgctcactac cgacctcgcg ctcgcgaagc tcgcgaaccg cagctggaca
     1921 ctcggcatcc tcggcccgtt cttcctgttc ggtgcactgg cggcgctgtt cggcgcgcgc
     1981 ggcacgctcg gcgaccggcg ggcgctgaag caggcgctgt cggatcgcgt gctgcgaccg
     2041 gtgccgctgc gcatggagca ctacg
//
LOCUS       DAOQJH010000567         2056 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_43666_length_2056_cov_4.645177, whole genome shotgun sequence.
ACCESSION   DAOQJH010000567 DAOQJH010000000
VERSION     DAOQJH010000567.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43666_length_2056_cov_4.645177"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..724
                     /locus_tag="PLQ18_11795"
     CDS             <1..724
                     /locus_tag="PLQ18_11795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02378.1"
                     /translation="SAAVDAQVRVLDFGHGELQSAVGHDLSAAAPSDFSPFTAGSNVT
                     GLFVSDYGANASADGFANHASTLAADGIRFTGDVAMYAAGSPGSPGIPSVSGLAEVLM
                     GVRFVLDEAATLELSLDSDPDGGFDFRLLGAGASVVWDQTSLYSPATGPQFTFVIHLP
                     LAAGEYRLDAALRAYSSFDNDTGYSATSFASFALTLPASVIAEPHPAMLLCAGLVATA
                     TAMRRRPRPLPLTTPLRTAAAA"
     gene            complement(690..1520)
                     /locus_tag="PLQ18_11800"
     CDS             complement(690..1520)
                     /locus_tag="PLQ18_11800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011471426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HNE02379.1"
                     /translation="MHIRIGYELTWDLPQPTPMILTLHVHPSRRADLIEPDRMQTQPA
                     VLTETYQDGFDNLCTRLVAPAGRFTVRSDALIRDPGTFDRCVPEAQQHPVETLPEETL
                     VFLLGSRYCETERLMPIAWQLFGHIQPGWARVQAICDYVHRHISFGYHHARATRTAWE
                     AHEERIGVCRDFTHLAVAFCRCLNIPARYCTGYLGDIGVPPGGPMDFSAWFEAYIGQH
                     WHTFDPRNNQPRIGRVLIARGRDASDVAISSTFGPNVLSGFRVVTEELKPQPQYAAAS
                     "
     gene            complement(1712..>2056)
                     /locus_tag="PLQ18_11805"
     CDS             complement(1712..>2056)
                     /locus_tag="PLQ18_11805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018076285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase C-terminal domain-containing
                     protein"
                     /protein_id="HNE02380.1"
                     /translation="LQAGEPVGYSGAWRCPRDTTIGVVAMGYADGYPRHAPSGTPVLV
                     NGRRTQVVGRVSMDMLCVDLGEQPGARIGDPVTLWGEGLPAELVAEAAGTIAYELFCN
                     LAARVHVRVIDG"
BASE COUNT          302 a          723 c          714 g          317 t
ORIGIN      
        1 ctcggccgcg gtcgatgcac aggtgcgcgt gctcgacttc ggtcacggcg aactgcagag
       61 cgccgtcggt catgatctct cggccgcggc ccccagcgat ttcagtccgt tcacggccgg
      121 cagcaacgtc accggcctgt tcgtatccga ctacggcgcc aacgccagcg ccgacggctt
      181 cgcgaatcac gcctcgacgc tggccgccga cggcatccgc ttcaccggcg acgtggcgat
      241 gtatgccgcc ggctcaccgg gctcgcccgg catcccgagc gtcagcgggc tggccgaagt
      301 gctgatgggg gtgcgcttcg tgctcgacga agcggccacg ctggaactgt cgctggacag
      361 cgatccggac ggcggtttcg acttccggct gctcggggcc ggcgcttcgg tggtctggga
      421 ccagaccagc ctctacagcc cggccaccgg cccgcagttc acgttcgtga tccacctgcc
      481 gctcgccgcc ggtgaatacc ggctcgatgc agcgctgcgg gcctattcga gcttcgacaa
      541 cgataccggc tactccgcca ccagcttcgc cagcttcgcg ctgacgctgc cggcgagcgt
      601 catcgccgag ccgcacccgg cgatgctgct ctgcgcgggt ctggtggcga cggcaaccgc
      661 catgcgcagg cgcccccggc ccttgccgct cacgacgccg ctgcgtactg cggctgcggc
      721 ttgagttcct cggtcacgac ccggaagccg ctcagcacgt tcggcccgaa ggtgctgctg
      781 atcgcgacat ccgaggcatc gcgtccgcgt gcgatcagca cgcggccgat gcgcggctgg
      841 ttgttgcgcg gatcgaaggt gtgccagtgc tggccgatgt aggcctcgaa ccaggccgag
      901 aagtccatcg gcccgcccgg cggcacgccg atgtcgccga gatagccggt gcagtagcgc
      961 gccgggatgt tcaggcagcg gcagaacgcg acggcgaggt gcgtgaagtc acggcagacg
     1021 ccgatgcgct cctcgtgcgc ttcccaggcc gtgcgcgtcg cgcgcgcatg gtgatagccg
     1081 aagctgatgt gccggtgcac gtagtcgcag atcgcctgca cgcgtgccca gcccggctgg
     1141 atgtggccga acagctgcca ggcgatcggc atcagccgtt cggtctcgca gtagcggctg
     1201 ccgagcagga ataccagcgt ctcctcgggc agggtctcga ccggatgctg ctgggcctcg
     1261 ggcacgcagc gatcgaaggt gccggggtcc cggatcagcg cgtcgctgcg caccgtgaag
     1321 cgcccggccg gtgccaccag ccgcgtacag aggttatcga agccgtcctg gtaggtctcg
     1381 gtcagcacgg ccggctgcgt ctgcatccgg tccggctcga tcagatcggc gcgacgggac
     1441 ggatgcacat gcagcgtcag gatcatcggc gtcggctgcg gaaggtccca ggtgagttcg
     1501 tatccgatgc gaatgtgcat ggcggtcagc gtctcggctc ggcggtcgca ggtgcgaccg
     1561 gcattggcgg aaaggacaga cccgccgcgc accgatccgg agcttcatga agcgcccgtc
     1621 gcctgcggcg ggccgcattc aaggcccctg ccggcattgg caaggatgcc ggcatgactg
     1681 ttccgaccgc aggctgccgg cggggttcgc gtcagccgtc gatcacgcgg acatgcacgc
     1741 gcgccgcgag gttgcagaag agttcgtagg caatcgtgcc ggcggcctcg gcgacgagtt
     1801 cggccggcag tccctcgccc cagagcgtca ccggatcgcc gatgcgcgcc ccgggctgct
     1861 cgccgagatc gacgcacagc atgtccatcg acacgcgccc gaccacctgc gtgcgccggc
     1921 cgttgaccag caccggcgtg ccgctcggcg cgtggcgcgg ataaccgtca gcgtagccca
     1981 tcgcgaccac gccgatcgtc gtatcgcgcg ggcagcgcca ggcgccggaa tagccgaccg
     2041 gctccccggc ctgcag
//
LOCUS       DAOQJH010000568         2053 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_43777_length_2053_cov_4.682683, whole genome shotgun sequence.
ACCESSION   DAOQJH010000568 DAOQJH010000000
VERSION     DAOQJH010000568.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2053
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43777_length_2053_cov_4.682683"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..337)
                     /locus_tag="PLQ18_11810"
     CDS             complement(<1..337)
                     /locus_tag="PLQ18_11810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GlxA family transcriptional regulator"
                     /protein_id="HNE02381.1"
                     /translation="MRTIGFVLADGFQVMSLAALSVFELANASLGDAGYALHLHSAGG
                     GPVRSSLGFGIDTRAFGPDSPDTIIVTGALVPDPAPAGVLDYLRDTGRGARRIAGICT
                     GAFVLAEAGL"
     gene            459..1262
                     /locus_tag="PLQ18_11815"
     CDS             459..1262
                     /locus_tag="PLQ18_11815"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017487294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNE02382.1"
                     /translation="MSAPAGTALITGASSGIGAVYAERLAGRGHDLILVARNVERLDA
                     LAGLLRTRHGVHVRTVSADLGTPAGVTTVEAVLRSDESITLLVNNAGFGSTAPLLQSD
                     PASMTQMIDVNVTALTRLTQAIAPGFVARGGGTIINIASIVAIGPELLNGVYGATKAY
                     VLALSQSLHKELAAHGVRVQVVLPGATATRFWDIAGLPVEHLPEAWVMSASDLVDAAL
                     IGLDRGEFATLPSLMDESEYTAWEAARQSMLGHLSNASPAERYRLTDRG"
     gene            1448..1672
                     /locus_tag="PLQ18_11820"
     CDS             1448..1672
                     /locus_tag="PLQ18_11820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011390943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="HNE02383.1"
                     /translation="MLTIELPAELEARLEALASEHGQSAGALVREAVLKLLDDLEDIA
                     AAEAALAEIEAGHGQPVPLEDVVKAFGLED"
     gene            1656..1925
                     /locus_tag="PLQ18_11825"
     CDS             1656..1925
                     /locus_tag="PLQ18_11825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011390944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HNE02384.1"
                     /translation="MAWKIELAPAALRALKQLDPPVARRLLSFLHQRLAPLDDPRSLG
                     AALQGARFEGLWRYRVGDYRIIARLQDDTLHILVVHIGHRRDVYR"
BASE COUNT          303 a          743 c          715 g          292 t
ORIGIN      
        1 gcaacccggc ctcggcgagc acgaaggcgc cggtgcagat gccggcgatg cgccgggcgc
       61 cgcggccggt gtcgcgcagg tagtcgagca cgcccgccgg tgccgggtcc ggcaccagcg
      121 cgccggtgac gatgatggtg tcggggctgt cgggcccgaa tgcccgggta tcgatgccga
      181 agccgagcga gctgcgcacg gggccaccgc cggccgagtg caggtgcagc gcgtagccgg
      241 cgtcgccgag gctggcgttc gccagctcga acaccgacag cgccgcgagc gacatgacct
      301 ggaaaccgtc tgccagcaca aaaccgatcg tgcgcatcgc cgcaacctcg atgtccgttt
      361 tcgatgttta tatgacattt gcgacatcgg cgcggcgcgg gatgctgagg gccgacgccg
      421 tccgctcccg gaccggcgca gcaccggagc ctccgaccat gtccgcacca gcgggtactg
      481 ccctcatcac cggcgcctcc tccggcatcg gcgccgtcta tgccgagcgc ctcgccggcc
      541 gcggccacga cctgatcctc gtcgcccgca acgtcgaacg gctcgacgcg ctggccgggc
      601 tgctgcgcac gcgccacggc gtgcacgtgc gcaccgtgag cgccgatctc ggcacgccgg
      661 ccggcgtgac gacggtcgag gcggtgctgc gcagtgacga gtcgatcacg ctgctggtca
      721 acaacgccgg cttcggcagc accgcgccgc tgctgcagtc cgatccggcg agcatgacgc
      781 agatgatcga cgtgaacgtc acggcgctga cccgcctgac ccaggccatc gcgccgggct
      841 tcgtcgcgcg cggcggcggc acgatcatca acatcgcctc gatcgtcgcg atcgggccgg
      901 agctgctgaa cggcgtctac ggcgcgacca aggcctacgt gctggccctc agccagtcgc
      961 tgcacaagga gctggccgcg cacggcgtgc gcgtgcaggt ggtgctgccg ggggcgaccg
     1021 ccacccgctt ctgggacatc gccggcctgc cggtcgagca tctgccggaa gcctgggtga
     1081 tgtcggcctc cgatctggtc gatgccgcgc tgatcggact ggatcgcggc gagttcgcga
     1141 cgctgccctc gctgatggac gaatccgagt acacggcctg ggaagccgcg cgccagtcga
     1201 tgctcggcca tctgtcgaac gcgagtccgg ccgagcgtta ccggctgacg gaccggggct
     1261 gagcgcagac ggcggatgcc tgcacagtga cctccggcag gcgctgcgcg gggggtgtcg
     1321 tgctgcctcc accctccgca cgcagggccc ggacggggat atagtcagcc cggtttcccg
     1381 cgcatgggcg ccggggtctt gccatcccgg cacgaacgcc acccggccgc tgacccggag
     1441 gtacacgatg ctgacgatcg aactgccggc cgaactcgaa gcccggctgg aggcgctggc
     1501 cagcgagcac ggccagagcg ccggagccct ggtccgggag gcggtgctga agctgctcga
     1561 cgatctggaa gacatcgcgg cggccgaggc cgccctggcc gagatcgaag ccggtcacgg
     1621 ccagccggtc ccgctcgaag acgtggtgaa agcgtttggc ctggaagatt gaactggcgc
     1681 cggcggctct cagggcgctg aaacagctcg atccgcccgt cgcacgccgc ctcctgagct
     1741 tcctgcacca gcgccttgca ccgctcgacg atccgcgcag cctgggcgct gccctgcagg
     1801 gagcgcgctt cgagggtctc tggcgctacc gggtcggcga ttaccggatc atcgcccgcc
     1861 tgcaggacga caccctgcac atcctcgtcg tccacatcgg tcatcggcgc gacgtctacc
     1921 gctgagcccg ccgttcacgg cacggtttcg tccgcaccct gcgcgtcgtt cgccgccagc
     1981 gcatcacgcg ccgcgatcgc ctcggcgagc gcgacgatgc gcgtcgcctc gatcacgtgc
     2041 aggtgctcga cca
//
LOCUS       DAOQJH010000569         2053 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_43778_length_2053_cov_4.600601, whole genome shotgun sequence.
ACCESSION   DAOQJH010000569 DAOQJH010000000
VERSION     DAOQJH010000569.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2053
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43778_length_2053_cov_4.600601"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            157..948
                     /locus_tag="PLQ18_11830"
     CDS             157..948
                     /locus_tag="PLQ18_11830"
                     /inference="COORDINATES: protein motif:HMM:NF012420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HNE02385.1"
                     /translation="MSLSIHIPKILGAAASAALLGMLLAPSGSHAEDHPAGPAHWSYE
                     GHDGPEHWATLDPKYATCSGGHSQSPINISDVQQSGLPPITFDYRKAAVDDVNNGHTV
                     QFQLANGSAITVAGRRYELLQAHFHEPSEHTVNGKSYPAEVHFVHRDANGALAVVGVL
                     IAEGTANRTLSTMWAYLPKAAGETQQGGKSSGFDPAGLLPKQREYYTYSGSLTTPPCT
                     EGVTWLLMQQPITASKAQIRQFGELFAMHPTNRPVQPLNGRVVAR"
     gene            1043..1717
                     /locus_tag="PLQ18_11835"
     CDS             1043..1717
                     /locus_tag="PLQ18_11835"
                     /inference="COORDINATES: protein motif:HMM:NF017102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HNE02386.1"
                     /translation="MSFRIPDSLSLRRMPRGLAALLLAAALAVPVQARADVAETVLPA
                     ACTRDCAAPWGETLGGSTTTPAYSNCSAACFVPLPNREQGIFTGIRWQCVEYARRWWL
                     LNRGVFFGDVDVAADLWLKIPAVTRASDGSTQPLENHDNGDSAPPKVGDLLVYGREYL
                     RTGHLAVVVAVDAGAGRIDVAEQNFLNRPWPAADHARSLDLLERKGRWWVLDPYLLGW
                     KRIAGE"
     gene            complement(1742..>2053)
                     /locus_tag="PLQ18_11840"
     CDS             complement(1742..>2053)
                     /locus_tag="PLQ18_11840"
                     /inference="COORDINATES: protein motif:HMM:TIGR04366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbuC family cupin fold metalloprotein"
                     /protein_id="HNE02387.1"
                     /translation="HRHLQPEKSEALIVLRGRLACFTFDESGTCTQRTVLAPGDGIDV
                     PPGEWHAVLALDPGTVFFETKAGPYRPLDEGERSAWAPGEQDDGAAAYRERLRGLCAN
                     H"
BASE COUNT          333 a          737 c          681 g          302 t
ORIGIN      
        1 cagcagggcc ggccagtgtg cggtgcagca tcggcacgaa tccgcaacat ccggtaaaag
       61 ccggaaattc cggttgtttc ccgaccgctc cccgcggact atgcgacgcc cgtcgcgcac
      121 gggcatcccg cataaaacga ggaaggggag tcaaacatga gcctgtcgat ccatattccg
      181 aaaatcctcg gtgcagcggc atccgcggcg ctgctgggca tgctgctcgc accgagcggc
      241 agccacgccg aggatcatcc ggccggtccg gcgcactgga gctacgaagg ccacgacggc
      301 cccgaacact gggcgacgct cgatccgaag tacgcgacct gctcgggcgg ccacagccag
      361 tcgccgatca acatcagcga cgtgcagcag tccggcctgc cgccgatcac cttcgactac
      421 cgcaaggcgg cggtcgatga cgtgaacaac ggccacaccg tgcagttcca gctcgccaac
      481 ggcagcgcga tcaccgtcgc cggccgtcgc tacgagctgc tgcaggccca cttccacgag
      541 ccgagcgagc acaccgtcaa cggcaagtcc tatccggccg aggtgcattt cgtgcaccgc
      601 gacgccaacg gcgcgctggc cgtggtcggc gtgctgatcg ccgaaggcac ggcgaaccgc
      661 acgctgtcga cgatgtgggc ctacctgccg aaggcggccg gcgagacgca gcagggcggc
      721 aagtcgagcg gcttcgatcc ggccggcctg ctgccgaagc agcgcgagta ctacacctac
      781 tccggctcgc tgacgacgcc gccgtgcacc gaaggcgtga cctggctgct gatgcagcag
      841 ccgatcaccg cgtcgaaggc gcagatcagg cagttcggcg agctgttcgc gatgcacccg
      901 accaaccgtc cggtacagcc gctgaacggg cgcgtcgtcg cccgctgagg ccttgccggc
      961 cccccggccg gtgcgcgttg acagcgccgg ccggcggcgg gcataccgca cggaccttcg
     1021 ccgccgtgcc gggcctgccg ccatgtcctt ccgcattccc gattccctga gcctgcgacg
     1081 catgccgcgc gggctggccg cgctgctgct cgccgccgcg ctcgccgtgc ccgtgcaggc
     1141 gcgggccgat gtcgccgaaa ccgtcctgcc ggcggcctgc acgcgcgact gtgccgcgcc
     1201 ctggggcgaa accctcggcg gcagcacgac cacgccggcc tattccaact gcagcgccgc
     1261 ctgcttcgtg ccgctgccga atcgcgagca ggggatattc accggcatcc gctggcagtg
     1321 cgtcgagtac gcacggcgct ggtggctgct gaaccgcggc gtgttcttcg gggatgtcga
     1381 tgtcgccgcc gatctgtggc tgaagatccc tgcggtcacg cgcgccagtg acggctccac
     1441 gcagccgctc gaaaaccacg acaacggcga cagcgccccg ccgaaggtcg gagacctgct
     1501 ggtctacggc cgcgagtacc tgcgtaccgg ccatctcgcg gtcgtcgtcg ccgtcgatgc
     1561 cggggccggg cgcatcgatg ttgccgaaca gaacttcctg aaccggccgt ggccggccgc
     1621 cgatcatgcg cgcagcctcg atctgctcga acgcaagggc cgctggtggg tgctcgatcc
     1681 gtatctgctc ggctggaagc gcatcgccgg cgaatgagca ggcgatgcga cagtgcgtgg
     1741 ttcagtggtt cgcgcagaga cctctcagcc gttcacggta cgccgcagca ccgtcgtcct
     1801 gctcgcccgg cgcccatgcg gaccgttcgc cttcgtccag gggccggtac ggaccggcct
     1861 tggtttcgaa gaacaccgtg cccggatcaa gcgccagcac cgcatgccat tcaccgggcg
     1921 gcacgtcgat gccatccccg ggcgccagca ccgtgcgctg cgtgcaggtg ccggattcgt
     1981 cgaacgtgaa acaggcaagc cggccgcgca gcacgatcag ggcttcggat ttttcgggct
     2041 gcagatggcg gtg
//
LOCUS       DAOQJH010000570         2053 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_43794_length_2053_cov_2.673674, whole genome shotgun sequence.
ACCESSION   DAOQJH010000570 DAOQJH010000000
VERSION     DAOQJH010000570.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2053
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43794_length_2053_cov_2.673674"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..643)
                     /locus_tag="PLQ18_11845"
     CDS             complement(<1..643)
                     /locus_tag="PLQ18_11845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013535418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HNE02388.1"
                     /translation="MNPSALPAAAPQPELVDSHSHFDVGEFDADRDAAYARARAAGVV
                     QQVVPGVIARSWDALDAVVARYPGLYAAWGLHPCYLAEHRPAHLAELRARVAARRPVA
                     IGECGLDAFLPQPDLPAQETYFLEQLRIARDAGLPVIVHARRAVDAVLKCIRRIGGRE
                     GLSGVVHSFAGSIEQAERLREHGFLVSLGGPLSYERAQRLHRLVRALPADGLLL"
     gene            778..1446
                     /locus_tag="PLQ18_11850"
     CDS             778..1446
                     /locus_tag="PLQ18_11850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HNE02389.1"
                     /translation="MRPSFPVLTMFAALAAAGPVAAGVLADFEGLATLPPVDGATGFE
                     FANGGSLDYAGVRWDDRIQVVGDAYRVDTATPGPLFGLPHSGSFFINNHNGADGILLE
                     TTQVLTGAWFARNEYYGFGGGADQVSITALGAGGDLLTLSLDLPELLAGLPEQPGFFD
                     TSAFLALSGITGYRIDRRETGPFSADWVADDFTFEAAVTAIPAPSVLALFGLGGLGLC
                     RRRR"
     gene            1534..1917
                     /locus_tag="PLQ18_11855"
     CDS             1534..1917
                     /locus_tag="PLQ18_11855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007865436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02390.1"
                     /translation="MKPHTLTFVLPALLLAACAASDPGAAPARVADGVWVGGNGMTLY
                     VFDKDVAGSGRSACNGACAANWPPLAAAADAPAGGDWTTIRRDDGSRQWAWRGRPLYF
                     WSKDTQPGQRSGDGFNQVWHVARAG"
     gene            complement(1924..>2053)
                     /locus_tag="PLQ18_11860"
     CDS             complement(1924..>2053)
                     /locus_tag="PLQ18_11860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005349518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNE02391.1"
                     /translation="IEDAIAACDGNIPKAAALLEISASTIYRKRQAWADADAAHSA"
BASE COUNT          261 a          723 c          763 g          306 t
ORIGIN      
        1 cgagcagcag accgtcggcc ggcagcgcgc gcaccagccg gtgcaggcgc tgcgcgcgct
       61 cgtagctcag cggtccgccg aggctgacca ggaagccgtg ctcgcgcagc cgctcggcct
      121 gctcgatcga gccggcgaag ctgtgcacga cgccgctcag gccctcgcgc ccgccgatgc
      181 ggcggatgca cttcagcacg gcatcgacgg cgcggcgggc gtgcacgatc accggcaggc
      241 cggcgtcgcg ggcgatgcgc agctgttcga ggaaatacgt ctcctgcgcg ggcaggtccg
      301 gctgcggcag gaaggcgtcg agcccgcact cgccgatcgc gaccgggcgg cgagcggcga
      361 cgcgcgcgcg cagttcggcc agatgtgcgg gccggtgctc ggcgaggtag cacgggtgca
      421 ggccccaggc tgcgtacagg ccggggtagc gcgcgaccac ggcatcgagc gcgtcccagc
      481 tgcgcgcgat gacgccgggc acgacctgct gcacgacgcc ggcggcgcgc gcgcgggcat
      541 aggcggcatc gcggtcggcg tcgaattcgc cgacatcgaa atggctgtgc gaatcgacca
      601 gttccggctg cggggccgca gcgggcaggg cggatggatt cacgggcatc ggatacggtc
      661 cttgccggac gcggacgccc ggcgtgctgg ctatgctgcc ggagtcgcag ccgcggagaa
      721 ctaggggatg accggccggc tgtctgtcac cggccgttcc cgtcgtggag gtccatgatg
      781 cgcccttcgt tccctgtact gacgatgttc gccgcgctgg ccgctgccgg tccggtcgcg
      841 gccggcgtgc tcgctgattt cgaaggtctg gcgacccttc cgccggtcga tggcgctacc
      901 ggcttcgagt tcgccaacgg cggcagcctc gattacgccg gcgtgcgctg ggatgaccgt
      961 attcaagtgg tcggcgatgc ctaccgcgtc gacaccgcaa cgccggggcc gctgttcggg
     1021 ctgccgcatt cgggcagctt cttcatcaac aaccacaacg gcgccgacgg catcctgctc
     1081 gaaaccacgc aggtgctgac cggcgcctgg ttcgcgcgca acgagtatta cggcttcggt
     1141 ggcggtgccg atcaggtgtc gatcaccgcg ctcggcgcgg gcggcgacct gctgacgctg
     1201 agcctcgatc tgccggagct gctggcgggc ctgcccgagc agccgggctt cttcgacacc
     1261 tcggccttcc tcgcgctgtc cggcatcacc ggctatcgca tcgaccggcg cgagaccggg
     1321 ccgttctcgg ccgactgggt cgccgatgac ttcacgttcg aggcggccgt gaccgcgatt
     1381 ccggcgccat cggtgctggc gctgttcggc ctcggcggcc tcgggctctg ccggcgccgg
     1441 cgctgaagcc gcggaagggt cgggcgggtc gcggtgtagc aggagctgcg gacagcgtcg
     1501 tccgtgccat ccgaaccttc cggagcctgc gccatgaagc cgcacacgct gaccttcgtc
     1561 ctgcccgccc tgctgctcgc cgcctgcgcc gccagtgatc ccggcgcggc tccggcccgc
     1621 gtcgccgatg gcgtctgggt cggtggcaac ggcatgacgc tgtacgtgtt cgacaaggat
     1681 gtcgccggtt ccggcaggag tgcctgcaac ggcgcctgcg ccgcgaactg gccgccgctc
     1741 gcggccgccg ccgatgcccc ggccggcggc gactggacga cgatccggcg cgacgacggc
     1801 agccgccagt gggcctggcg gggccggccg ctgtacttct ggagcaagga cacgcagccg
     1861 ggccagcgca gcggcgacgg cttcaaccag gtctggcacg tcgcgcgcgc cggctgaggc
     1921 cgctcaggcg ctgtgcgcgg cgtcggcgtc ggcccaggcc tggcgcttgc ggtagatggt
     1981 cgaggcgctg atctccagca gcgccgcggc cttcgggatg ttgccgtcgc aggcggcgat
     2041 ggcatcctcg atc
//
LOCUS       DAOQJH010000571         2047 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_44031_length_2047_cov_4.267068, whole genome shotgun sequence.
ACCESSION   DAOQJH010000571 DAOQJH010000000
VERSION     DAOQJH010000571.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2047
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44031_length_2047_cov_4.267068"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..347)
                     /locus_tag="PLQ18_11865"
     CDS             complement(<1..347)
                     /locus_tag="PLQ18_11865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013345745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HNE02392.1"
                     /translation="MFTAIVALSGLATLFGCLLGYASVRFRVEGDPLAERLDAVLPQT
                     QCGQCGFAGCKPYAEALAKGEAAIDLCAPGGQPGVVAIAGVLGVEPTSAANDGEPVRM
                     HAVIHEEGCVGCTV"
     gene            complement(353..934)
                     /gene="rsxA"
                     /locus_tag="PLQ18_11870"
     CDS             complement(353..934)
                     /gene="rsxA"
                     /locus_tag="PLQ18_11870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013220989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HNE02393.1"
                     /translation="MKDYVLILIGTILVNNFVLVKFLGLCPFMGVSKKVETALGMGMA
                     TTFVLTLSSACTWFMYSAVLVPLGLEYLNTIAFILVIAVVVQFTEMVVHKTSPVLYQV
                     LGIYLPLITVNCAVLGVALLNVQAHHDFLGATLYGFGAAAGFSLVLVLFAALRERIAV
                     ADVPLPFRGPAIALVTAGLMSLAFLGFTGFVRG"
     gene            complement(1326..>2047)
                     /gene="argF"
                     /locus_tag="PLQ18_11875"
     CDS             complement(1326..>2047)
                     /gene="argF"
                     /locus_tag="PLQ18_11875"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006788244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HNE02394.1"
                     /translation="LGGSAIFLSPRDAQLGRGESIEDTARVISRMVDLVMIRAFRHET
                     VTRFAAASQVPVINGLTDHAHPCQLLADIQTWSELRGPVAGRTVAWVGDGNNMCNSYL
                     TAAELFDFRLRIACPEGYEPDAALLARHADRVELLRDPLAAAAGADLVTTDVWASMGH
                     EDEMEKRAAAFAGYCVDAAVMAAAAPDAIFLHCLPAHRGEEVAAEVIDGSQSAVWEEA
                     ENRMHAQKALMEFLLLGEQAD"
BASE COUNT          362 a          668 c          721 g          296 t
ORIGIN      
        1 accgtgcagc cgacgcagcc ttcctcgtga atcacggcgt gcatgcgcac cggctcgccg
       61 tcgttggccg ccgaggtcgg ctcgacgccg agcacgccgg cgatcgcgac cacgccgggc
      121 tggccgccgg gcgcgcacag gtcgatcgcc gcctcgcctt tcgcgagcgc ttcagcgtag
      181 ggcttgcagc cggcgaagcc gcactggccg cactgcgtct gcggcagcac ggcgtcgagg
      241 cgttcggcga gcggatcacc ttcgacgcga aagcgcaccg aggcataacc gagcaggcat
      301 ccgaacagtg tggcgagtcc ggagagggcg acgatggcag tgaacatggg aatcaacccc
      361 gcacgaaacc ggtgaagccg aggaaggcga gcgacatcag gcccgcggtg acgagtgcga
      421 tggccggacc gcggaacggc agcggcacat cggcaacggc gatgcgttcg cgcagcgccg
      481 caaacagcac gagcacgagc gagaagccgg cggcggcgcc gaagccgtac agcgtcgcgc
      541 cgaggaaatc gtgatgcgcc tgcacgttca gcagcgccac gccgagcacc gcgcagttga
      601 ccgtgatcag cggcaggtag atgccgagca cctgatagag cacggggctg gtcttgtgca
      661 cgaccatttc cgtgaactgc acgacgacgg cgatgaccag gatgaaggcg atcgtgttca
      721 ggtattcgag cccgagcggc accagcacgg cgctgtacat gaaccaggtg caggccgagg
      781 acagcgtcag cacgaaggtc gtcgccatgc ccatgccgag cgcggtctcg accttcttcg
      841 agacgcccat gaacgggcag agaccgagaa acttcacgag cacgaagttg ttgacgagaa
      901 tcgtgccgat caggatcagg acgtagtctt tcatggttcc gccaggtccc cggtcaccgg
      961 ttgattgccg ccgccggttg cagtgcgacc ggcggtccct tcaataaacc attacggcgt
     1021 catggttttc ggccttgctc cgcctgcctt gcagcctacg gcagggcggc ggtgccaaaa
     1081 catgacgcag cgcaacagca ggcggtttcg cgagcgatcc gtgcgtggtt cgccggcatg
     1141 aaacttgata aggagctgcc tgtcgccgtg tggcgcgagc cggcgggcga aataccccac
     1201 aaactgcacg gcccggtcgg ggaccgggcc ggagcgattc gcattacggc tgcaagcggc
     1261 tgattctagc gaagtaccgg tgcggcggac aaggcaggtg ccgccaccgg tgcggcccgg
     1321 cggcctcagt cggcctgctc gccgagcagc aggaactcca tcagcgcctt ctgcgcatgc
     1381 atgcgatttt ccgcttcctc ccagacggcg ctctgcgatc cgtcaatcac ctcggccgcg
     1441 acttcctcgc cgcggtgcgc gggcaggcag tgcaggaaga tcgcatccgg cgcggccgcg
     1501 gccatcacgg cggcatcgac gcagtagccg gcgaaggcgg ctgcgcgttt ctccatctcg
     1561 tcctcgtggc ccatgctcgc ccagacgtcg gtcgtgacca gatcggcccc ggcggcggcg
     1621 gcgagcggat cgcgcagcag ctcgacgcga tcggcgtggc gcgcgagcag cgcggcatcg
     1681 ggctcgtagc cctcggggca ggcgatgcgc agccggaagt cgaacagctc ggcggcggtg
     1741 aggtagctgt tgcacatgtt gttgccgtcg ccgacccagg cgaccgtgcg cccggcgacc
     1801 gggccgcgca gttcgctcca ggtctggatg tcggcgagca gctggcaggg atgggcatgg
     1861 tcggtcaggc cgttgatgac cggcacctgc gaggcggcgg cgaagcgcgt gacggtttcg
     1921 tggcggaagg cgcggatcat gaccagatcg accatgcgcg agatcacgcg cgcggtgtcc
     1981 tcgatcgatt cgccgcgccc gagctgcgca tcgcgcggcg acaggaagat cgccgagccg
     2041 ccgagct
//
LOCUS       DAOQJH010000572         2047 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_44050_length_2047_cov_2.747490, whole genome shotgun sequence.
ACCESSION   DAOQJH010000572 DAOQJH010000000
VERSION     DAOQJH010000572.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2047
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44050_length_2047_cov_2.747490"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1021
                     /locus_tag="PLQ18_11880"
     CDS             <1..1021
                     /locus_tag="PLQ18_11880"
                     /inference="COORDINATES: protein motif:HMM:NF024589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNE02395.1"
                     /translation="ENLESVALYGGEQVEDGRLRTRFRRVIDNYHRLIIAGFKVDMFN
                     AGYSQVSVIVPYLTVAPFYFATKMPFGQLMQIGNAFNRVQDAMSFFIDSYQNIAAWRA
                     VTLRLTQFTEALESLEARIDDISQVERSSAPRPQTETLGIRRPDGEALLDGLDLDLQP
                     GESLLISGASGSGKSTLLRTLAGLWPYAKGRVRLPGDGVLFVPQKPYLPIGTLREALL
                     YPQQQTVSDDALQAVLAEVGLERLAGQLETVEDWQQTLSGGEQQRVAFARILLRRPRW
                     LFLDEASSALDEPSEQTLYALLRARLPETTIVSIGHRSTLAAFHRRRLQLDGRGGWQL
                     ADVPA"
     gene            1156..>2047
                     /locus_tag="PLQ18_11885"
     CDS             1156..>2047
                     /locus_tag="PLQ18_11885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HNE02396.1"
                     /translation="MSSTPQDPAPGAGGTLVFWHPHCSEHDTGSGHPERAARMQAVLD
                     ALGAPEFRGLQWREAPAASFEQLERVHPAHHVDRLMSRMPAWGQAMLDGDTIVSPGSF
                     EAALRAAGAVCAAVDAVVAGEAANAFCAIRPPGHHAEPARAMGFCLFNNVAVGAEHAR
                     RVHGIERVAIVDFDVHHGNGTQAIFEAVPQVLYVSTHQYPLYPGTGHAEETGVAANVL
                     NVPLRAGSGGEALRRAWNEEILPALRRHEPGLLLVSAGFDAHRDDPLAELAFTEADYQ
                     WLAAQLAAFAAECCGGRLVSV"
BASE COUNT          290 a          744 c          720 g          293 t
ORIGIN      
        1 cgagaacctc gaatcggtgg cgctgtacgg cggcgagcag gtcgaggacg ggcggctgcg
       61 cacgcgcttt cgccgcgtca tcgacaatta ccaccggctg atcatcgccg gcttcaaggt
      121 cgacatgttc aacgccggct acagccaggt gtcggtgatc gtgccctacc tgaccgtcgc
      181 gccgttctac ttcgcgacga agatgccgtt cggccagctg atgcagatcg gcaacgcctt
      241 caaccgcgtg caggacgcga tgagcttctt catcgacagc taccagaaca tcgccgcgtg
      301 gcgcgccgtg acgctgcgcc tgacgcagtt caccgaggcg ctggaatcgc tggaggcgcg
      361 catcgacgac atctcgcagg tcgagcgcag cagcgcgccg cgcccgcaga ccgagacgct
      421 cggcatccgc cgcccggacg gcgaggcgct gctcgacggg ctcgatctcg acctgcagcc
      481 cggcgagtcg ctgctgatca gcggtgcctc gggcagcggc aagagcacgc tgctgcgcac
      541 gctggccgga ctgtggccct acgcgaaggg ccgcgtgcgc ctgcccggtg acggcgtgct
      601 gttcgtgccg cagaagccct acctgccgat cggcacgctg cgcgaggcgc tgctgtaccc
      661 gcagcagcag acggtgagcg atgacgcctt gcaggccgtg ctcgccgagg tcggtctgga
      721 gcggctcgcc ggccagctcg aaacggtcga ggactggcag cagacgctct cgggcggcga
      781 gcagcagcgc gtcgccttcg cacgcatcct gctgcgccgg ccgcgctggc tgttcctcga
      841 cgaggcgagt tccgcgctcg acgaaccctc cgaacagacg ctgtatgcgc tgctgcgcgc
      901 acggctgccg gaaacgacga tcgtcagcat cggccaccgc agcacgctcg cggccttcca
      961 ccggcgccgg ctgcagctcg acggccgcgg cggctggcag ctcgccgacg tgccggcctg
     1021 aggtggcgcg ttcctgccgc ctgtgggcga cctgaccggc gtcaatcgtt acacgccggt
     1081 gtcggcctac gctcgacata tgcttgccgt atcgcgcccg ctcgaaggcg ccggcatccg
     1141 caggaggagc agaccatgtc aagcacgccc caagacccgg cgcccggcgc cggcggcacg
     1201 ctggtcttct ggcacccgca ctgctcggag cacgacaccg gcagcgggca tccggagcgg
     1261 gccgcgcgca tgcaggcggt gctcgatgca ctcggggcgc cggaattccg cggcctgcag
     1321 tggcgcgagg cgccggcggc gagcttcgag cagctcgaac gcgtgcaccc ggcgcaccac
     1381 gtcgaccggc tgatgagccg catgccggcc tggggacagg cgatgctcga tggcgacacc
     1441 atcgtctcgc ccggcagctt cgaggcggcg ctgcgtgccg ccggtgccgt gtgcgcggcg
     1501 gtcgatgccg tcgtcgccgg tgaggccgcc aacgccttct gcgcgatccg cccgccgggc
     1561 caccacgccg agcccgcgcg cgcgatgggc ttctgcctgt tcaacaacgt cgcggtcggc
     1621 gccgagcacg cgcggcgcgt gcacggcatc gagcgcgtcg cgatcgtcga tttcgacgtg
     1681 caccacggca acggcacgca ggcgatcttc gaggccgtgc cgcaggtgct gtacgtgtcg
     1741 acgcaccagt acccgctgta ccccggcacc ggtcacgccg aggaaaccgg cgtcgccgcc
     1801 aacgtgctga acgtgccgct gcgcgccggc tccggcggcg aggcgctgcg tcgcgcctgg
     1861 aacgaggaga tcctgccggc gctgcgccgc cacgaacccg gcctgctgct ggtctcggcc
     1921 ggtttcgatg cgcaccgcga tgatccgctc gccgaactgg ccttcaccga ggccgactac
     1981 cagtggctgg ccgcgcagct cgccgcgttc gcggccgagt gctgcggcgg gcggctggtg
     2041 tcggtgc
//
LOCUS       DAOQJH010000573         2045 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_44104_length_2045_cov_5.946231, whole genome shotgun sequence.
ACCESSION   DAOQJH010000573 DAOQJH010000000
VERSION     DAOQJH010000573.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2045
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44104_length_2045_cov_5.946231"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1149
                     /locus_tag="PLQ18_11890"
     CDS             <1..1149
                     /locus_tag="PLQ18_11890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02397.1"
                     /translation="TAVLMPGAAMAIFHAIETPRRRGVWLIGAGVLTGLAYSSKDTAL
                     LMLPPLVLWLLIGMRDVGPLRRLQAAALYGLAMLAVYLAESAAYWWVADDFFYRSHAL
                     TQVHNRTIEASAGALEFAKAGYWALLTLLVDWARNGPMLVLGALGALGVLLWNRPLAG
                     FVLTGLFVTLYLIVGSSSLTRLVHLPIQDRYLLPCIPFAAIALGLALDAVGRRWPGRF
                     DITGAATIPLLIALLATGVKSAAAEAGSITFADYYRNAADAIRILSANGRPIHVDSVL
                     HHHLRHMLPPELHRQLVVFDEMPSLAPGYYMIVPMLLPESHASKETQMQLERMSPALP
                     ALIASIVTQPVVMRITTSHFDAGRYSATARDQPLMRAYKAAIVHAVGP"
     gene            1236..>2045
                     /locus_tag="PLQ18_11895"
     CDS             1236..>2045
                     /locus_tag="PLQ18_11895"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018898578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNE02398.1"
                     /translation="MTLPVHSEADYRIAVLIPCYNEAVTIGEVVTDFRRALPDATIYV
                     FDNNSVDDTAAIAHSVGADVRTVRQQGKGNVVRRMFADVEADLYVMVDGDGTYDADSA
                     AAMVRKLIGEHADMVVGCRHTEERSAYRAGHRFGNRLLSGCVAMLFGRSFTDILSGYR
                     VFSRRFVKSFPALAAGFEIETELTVHALELRMPIGEFDTPYRSRPAGSTSKLSTYRDG
                     WRILMTILKLMKSERPLSFFGAVCALLLLLSVALAAPIVQTAEAVARVLGFA"
BASE COUNT          313 a          731 c          643 g          358 t
ORIGIN      
        1 accgccgtac tgatgccggg cgcggcgatg gccatcttcc acgcgatcga aaccccgcgg
       61 cgacgcggcg tgtggctgat cggcgccggc gtgctgaccg gactcgccta ttcctcgaag
      121 gacacggcgc tgctgatgct gccgccgctc gtgctgtggc tcctgatcgg catgcgtgac
      181 gtcggcccgc tgcgccggct gcaggctgcc gcgctgtacg gactcgcgat gctcgcggtc
      241 tacctcgccg aaagtgcagc gtactggtgg gtcgccgacg acttcttcta tcgcagccac
      301 gcactgaccc aggtccacaa ccggaccatc gaggcgtccg ccggagcgct cgaattcgcc
      361 aaggccggat actgggcgct gctgacgctg ctcgtggact gggcgcgcaa cggtccgatg
      421 ctggtcctcg gcgcgctcgg cgcactcggc gtgctgctct ggaaccggcc tctcgccggt
      481 ttcgtgctga ccggcttatt cgtgacgctg tacctgatcg tcggctcgtc gagcctcacg
      541 cgcctggtcc acctgccgat ccaggaccgc tatctgctgc cgtgcattcc attcgcagcc
      601 atcgccctcg gtctggcgct tgatgccgtc ggccgacgct ggccgggccg gttcgacatc
      661 accggcgcag cgaccatacc cctgctgatc gcgctgcttg cgaccggcgt gaagtccgcg
      721 gcagccgagg ccgggtcgat cacgttcgcc gactattacc gcaatgcggc cgacgccatc
      781 cgcatcctgt ccgcgaacgg ccgcccgatc cacgtggaca gtgtgctgca ccatcacctg
      841 cgccacatgc tgccgcccga actgcaccgg caactcgttg tgttcgacga aatgccgtcg
      901 ctggcgcctg ggtattacat gatcgtgccg atgctgctgc ccgagagcca cgcgagcaag
      961 gaaacccaga tgcaactgga gcggatgtct cccgcactgc cggccctgat cgcaagcatc
     1021 gtcacgcagc cggtggtcat gcgcatcacg acgagccatt tcgatgccgg acgctattcg
     1081 gcgaccgcgc gcgaccagcc gctcatgcgc gcgtacaagg ccgccatcgt gcatgccgtc
     1141 ggcccctgaa catcgattcc ccaatggccg gcgccgaatt gcgcgatgcc ggcaccgccc
     1201 cagaaccgtt cgcctgccgg agagtttccc cgatcatgac gctgcccgtg cattccgaag
     1261 ccgattaccg gatcgccgtc ctgattccct gctacaacga ggccgtcacg atcggtgagg
     1321 tcgtgactga ctttcgccgc gcgctgcccg atgccacgat ctacgtgttc gacaacaact
     1381 ccgtcgacga cacggccgcc atcgcgcact cggtcggcgc cgatgtgcgc acggtgcgcc
     1441 agcagggcaa gggcaacgtg gtgcggcgca tgtttgcaga cgtcgaagcc gatctctacg
     1501 tgatggtcga cggcgacggc acctatgacg ccgacagcgc cgcggcgatg gtgcgcaagc
     1561 tcatcgggga gcacgcggac atggtggtcg gttgccggca caccgaggaa cgctcggcct
     1621 accgcgccgg acaccgcttc ggcaaccgcc tgctgagcgg ctgcgtcgcc atgctgttcg
     1681 ggcgcagctt caccgacatc ctgtcgggct accgcgtgtt ctcgcgacgc ttcgtcaagt
     1741 ccttcccggc gctcgccgcg ggtttcgaga tcgaaaccga actgaccgtc cacgcgctcg
     1801 aactgcgcat gccgatcggc gagttcgaca cgccgtaccg cagccggccc gccggctcga
     1861 cgagcaagct cagcacctac cgggacggct ggcgcatcct gatgacgatc ctgaaactca
     1921 tgaagtcgga acggccgctg agcttcttcg gtgcggtctg cgcgctgctg ctgctgctgt
     1981 ccgtggcgct cgccgccccg atcgtgcaga ccgcagaagc cgtggcacgc gtgctcgggt
     2041 tcgcc
//
LOCUS       DAOQJH010000574         2040 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_44330_length_2040_cov_3.890680, whole genome shotgun sequence.
ACCESSION   DAOQJH010000574 DAOQJH010000000
VERSION     DAOQJH010000574.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2040)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44330_length_2040_cov_3.890680"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1410)
                     /locus_tag="PLQ18_11900"
     CDS             complement(<1..1410)
                     /locus_tag="PLQ18_11900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008649736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2252 domain-containing protein"
                     /protein_id="HNE02399.1"
                     /translation="MSDAVDPELRQQRGKALRKRASRKQHDHIGGHDRDPVALLEANS
                     RTRLPRLLPLRYGRMLASPFAFFRGSAIVQAHDLAGTPYSGLIVPLCGDCHLMNFGGF
                     ATPERNLIFDVNDFDETHPGPWEWDVKRLAASFHIAARHQGYAEHDCADIVAAALDSY
                     REHTKTYARCSELELWYDRISFERLIQEAEDPDMRAQLAQTVEKASGRTHEHLLPKLG
                     SRTGDHWTMHDAPPGLFHIHGPASLFEPDDEWRRPGDWQRLIQPMYAAYLDTVSSSHR
                     ALLGRFRAQDLAFKVVGVGSVGTRCLVLLLTDGHDSPLFLQFKEALPSVLADYVPAGR
                     CPYRHQGRRVVAGKRLMQASSDIFLGWTTAFDGRQFYVRQLRDMKVSPEIETYDEAQL
                     HYYAASCGRVLARGHARAGGLAPEIAGYLGNGDAYTAAVLDYARDYADRVEQDFEHFR
                     AACRSGRLQAQTEADLAAEF"
     gene            1510..>2040
                     /locus_tag="PLQ18_11905"
     CDS             1510..>2040
                     /locus_tag="PLQ18_11905"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HNE02400.1"
                     /translation="MPVRVFISYSHDSEAHRQRVLAFVQALRGHGIDADLDQFHGETI
                     VHWPTWCRQRMDPEHSDFVLCICTAGYRQRINGNVAPEQGRGVYWEGALMQSELYDGK
                     GNRRFLPVLFDDEPEQSIVECLRGWTQCRVRQHALTDDGYELLLRILTNQPRATPDAL
                     GQVPEFKPAPALAQRPT"
BASE COUNT          305 a          656 c          753 g          326 t
ORIGIN      
        1 gaactccgcc gccagatcgg cttcggtctg cgcctgcagc cggccgctgc ggcaggcggc
       61 ccggaagtgt tcgaagtcct gctcgacgcg atcggcgtag tcgcgcgcgt agtcgagcac
      121 ggccgcggtg tacgcatcgc cgttgccgag gtagccggcg atctccgggg cgaggccgcc
      181 ggcgcgggcg tggccgcggg ccagcacccg gccgcagctc gcggcgtagt agtgcagttg
      241 tgcctcgtcg taggtctcga tctccgggga caccttcatg tcgcgcaact ggcgcacgta
      301 gaactggcga ccgtcgaagg ccgtggtcca gccaaggaag atgtcgctcg atgcctgcat
      361 caggcgcttg ccggcgacca cgcgccggcc ctgatgccgg tacgggcagc gcccggccgg
      421 cacgtagtcc gccagcaccg agggcagcgc ctccttgaac tgcaggaaca gcgggctgtc
      481 gtggccgtcg gtcagcagca gcaccagaca gcgcgtgccg acgctgccga cgccgaccac
      541 cttgaaggcg agatcctgcg cgcggaagcg cccgagcagc gcgcggtggc tgctgctgac
      601 cgtatcgaga taggccgcgt acatcggctg gatcagccgt tgccagtcgc cgggccggcg
      661 ccattcatcg tcgggctcga acaggctggc cgggccgtgg atgtggaaca ggcccggcgg
      721 tgcgtcgtgc atcgtccagt gatcgcccgt tctgctgccg agcttcggca gcaggtgttc
      781 gtgcgtgcgc ccgctggctt tctcgaccgt ctgtgcgagc tgcgcgcgca tgtccgggtc
      841 ttcggcttcc tggatcaggc gctcgaagct gatgcggtcg taccagagtt cgagttcgct
      901 gcagcgcgcg taggtcttcg tgtgttcgcg gtagctgtcg agggcggcag cgacgatgtc
      961 ggcgcagtcg tgttcggcgt agccctggtg gcgggcggcg atgtggaagc tggcggcgag
     1021 gcgcttcacg tcccattccc aggggccggg gtgggtttcg tcgaagtcgt tgacatcgaa
     1081 gatcaggttg cgctcgggcg tggcgaagcc gccgaagttc atcaggtggc agtcgccgca
     1141 gagcggcacg atcaggccgc tgtacggcgt gccggccaga tcatgcgcct gcacgatcgc
     1201 cgagccgcgg aagaacgcga acggcgatgc gagcatgcgc ccgtagcgca gcggcagcag
     1261 ccgcggcagc cgcgtgcggc tgttcgcctc cagcagcgcg accggatcgc ggtcatgccc
     1321 gccgatgtga tcgtgctgct tgcgcgaggc ccgcttgcgc agggccttgc cgcgttgctg
     1381 gcgcagttcg ggatcaacgg catcgctcat cgaagggact cccggtggca cggctctggt
     1441 gggcggaggg taaatcaggg ctgcgcggcg ttaggatcgg ccggcgaggg aaggggaggg
     1501 acgcgcgcaa tgccggtacg ggtgttcatc agctacagcc acgacagtga agcgcatcgc
     1561 cagcgggtgc tggcgttcgt gcaggcgctg cgcgggcacg gcatcgatgc cgatctcgac
     1621 cagttccacg gcgagaccat cgttcactgg ccgacgtggt gccggcagcg gatggacccc
     1681 gagcattcgg atttcgtgct ctgcatctgc acggccggat accggcagcg catcaacggc
     1741 aacgtggcgc cggagcaggg caggggcgtg tactgggaag gcgcgctgat gcagtccgag
     1801 ctgtacgacg gcaagggcaa ccgccgcttc ctgccggtgc tgttcgacga cgagccggag
     1861 cagagcatcg tcgagtgcct gcgcggctgg acgcagtgcc gggtccggca gcacgcgctg
     1921 accgatgatg gctacgaact gctgctgcgc atcctgacca atcagccccg cgccacgccc
     1981 gatgcactcg ggcaagttcc ggagttcaag cccgcgccgg cactcgcgca gcggccgaca
//
LOCUS       DAOQJH010000575         2036 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_44511_length_2036_cov_3.147905, whole genome shotgun sequence.
ACCESSION   DAOQJH010000575 DAOQJH010000000
VERSION     DAOQJH010000575.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44511_length_2036_cov_3.147905"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..370)
                     /locus_tag="PLQ18_11910"
     CDS             complement(<1..370)
                     /locus_tag="PLQ18_11910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02401.1"
                     /translation="MAGIDPELPARWLDIQRRLWAPWLEATGKPNGGRAAGTADLSGL
                     PAFFEAQARQWLEFQRECARALMQSGGGDPAHIAALLDAQARAAEGLLHFQRQCLDGW
                     LHAAGSFDWMQWLGSLQRLPG"
     gene            complement(502..702)
                     /locus_tag="PLQ18_11915"
     CDS             complement(502..702)
                     /locus_tag="PLQ18_11915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02402.1"
                     /translation="MNIATLDRLAQLQDVERAVIDLLFADRTLETCSRERLAVLLEFI
                     HEARSEALASVHSEPANVVTLR"
     gene            complement(880..1008)
                     /locus_tag="PLQ18_11920"
     CDS             complement(880..1008)
                     /locus_tag="PLQ18_11920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02403.1"
                     /translation="MSGLALLAIVAASLALGGLWNHLFLKDIGWRTPRAPHVRPQH"
     gene            1190..1279
                     /gene="kdpF"
                     /locus_tag="PLQ18_11925"
     CDS             1190..1279
                     /gene="kdpF"
                     /locus_tag="PLQ18_11925"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HNE02404.1"
                     /translation="MSLIHLLAAIVAAALALYLLAALCWPERF"
     gene            1292..>2036
                     /locus_tag="PLQ18_11930"
     CDS             1292..>2036
                     /locus_tag="PLQ18_11930"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF015753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HNE02405.1"
                     /translation="MTGNGLAQIALYFLVLTGLAVPLSAWMERIYAGRAGWLETVLGP
                     LERGLYRLIGPAAGQAQDWRSYAFAVLVFSGAGIVLLFVLQLAQAALPLNPQAQPAVP
                     AALALNTAVSFVTNTNWQNYAGEATLGYAVQMLGLTVQNFLSAATGLAVMLALFRGFT
                     QRESATLGNFWVDLVRSVLYLLLPLALGFALVLVACGVVQSFAPYVQATLLQPLADAD
                     GHTLGSQTIALGPAASQVAIKQLGTNGGGF"
BASE COUNT          278 a          694 c          741 g          323 t
ORIGIN      
        1 ggcccggcag gcgctgcagc gagccgagcc actgcatcca gtcgaagctg ccggccgcgt
       61 gcagccagcc gtcgaggcac tggcgctgga agtgcagcag gccctcggcc gcacgcgcct
      121 gcgcatcgag cagggccgcg atgtgggccg gatcgccgcc gccggactgc atcagcgcgc
      181 gcgcgcattc gcgctggaat tccagccact ggcgtgcctg cgcctcgaag aaggccggca
      241 gcccgctcag gtcggcggtg ccggcggccc ggccgccgtt gggcttgccg gtcgcttcga
      301 gccacggggc ccacagccgg cgctggatgt cgagccagcg tgccggcagt tccggatcga
      361 ttcccgccat cgtcgtctct cctctgcgtg gttcttcttc gggatccgca cgtacggccg
      421 cagccgtccg ccaggggata ccgcgcagca tagtcgagcc cggtgaccgg ggttggcggt
      481 gcgaatgccg gggcagccgc ctcagcgcag ggtcacgacg ttggccggtt cgctgtgcac
      541 ggaagcgagc gcttccgagc gcgcctcatg aatgaattcg agcagcacgg cgaggcgttc
      601 gcggctgcag gtttccagcg tgcggtcggc gaacagcagg tcgatgacgg cgcgttcgac
      661 gtcctggagc tgggcgaggc gatcgagggt agcaatgttc atggtcatgg tccgcggtcc
      721 cggaattgct gcggggcaac gggacgcagg atacgggcca ccgcgcgcgc cgtccgccgc
      781 gtgcggtcac gtccggtcac accggcgtta agaaacgtcg cagcacggaa cttgcggatg
      841 gcgcaaaacg aagcgccggg ctgaccttcc ggtgcaacgt caatgttgcg ggcgcacatg
      901 cggtgcacgc ggcgtccgcc agccgatgtc cttcaggaac agatggttcc acaggccgcc
      961 gagcgcaagg ctggcagcga cgatcgcgag cagggcgagt ccggacatga tgcacctccg
     1021 tatggcgcgg gctggcgcgg gatgcgcgta ccccgccggg tggcgtatgc gctccagcat
     1081 cggtgacagg ggcgttacgg aaccattgcc gcggcggccc cggcgttaac ggcttcttta
     1141 cgggccgcac ggccagactg cgcgcagcgt ttccaccgga gtcccgccca tgagcctgat
     1201 ccacctgctc gctgcgatcg tcgccgcggc cctcgcgctg tatctgctcg ccgcgctctg
     1261 ctggcccgag cgcttctgaa ggcgcgccgc gatgaccggc aacgggcttg cgcagatcgc
     1321 gctgtacttc ctcgtgctca ccgggctcgc cgtaccgctg tcggcctgga tggagcgcat
     1381 ctacgccggc cgggccggct ggctcgaaac cgtgctcggg ccgctcgaac gcggactgta
     1441 ccggctgatc ggcccggcgg cgggccaggc gcaggactgg cgcagctacg cgttcgccgt
     1501 gctggtgttc tcgggcgccg gcatcgtgct gctgttcgtg ctgcagcttg cgcaggcggc
     1561 gctgccgctg aacccgcagg cgcagccggc ggtgccggcg gcgctggcgc tgaacacggc
     1621 ggtcagcttc gtcaccaata ccaactggca gaactacgcc ggcgaggcga cgctcggcta
     1681 cgcggtgcag atgctcgggc tgacggtgca gaacttcctg tcggcggcga ccgggctggc
     1741 ggtgatgctg gccttgtttc gcggcttcac gcagcgcgag agcgcgacgc tcggcaactt
     1801 ctgggtcgat ctggtgcgca gcgtgctgta cctgctgctg ccgctggcgc tggggttcgc
     1861 gctggtgctg gtcgcctgcg gcgtcgtgca gagcttcgcg ccctacgtgc aggcgacgct
     1921 gctgcagccg ctcgccgatg ccgatggcca cacgctcggc agccagacga tcgcgctcgg
     1981 accggcggcc tcgcaggtcg cgatcaagca gctcggcacc aacggcggcg gcttct
//
LOCUS       DAOQJH010000576         2034 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_44575_length_2034_cov_3.525518, whole genome shotgun sequence.
ACCESSION   DAOQJH010000576 DAOQJH010000000
VERSION     DAOQJH010000576.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44575_length_2034_cov_3.525518"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..797)
                     /locus_tag="PLQ18_11935"
     CDS             complement(<1..797)
                     /locus_tag="PLQ18_11935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013819675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HNE02406.1"
                     /translation="MKRILIAHRTEYRYPEPVRLGPHRLLLRPREGHEVRVERSRLDI
                     TPAAQLRWCRDADDNSVAIAHFPQPAGRLCIDSEVVIEHHDDAPLDFLVEDYALHYPF
                     EYAAAERTDLQLASTPVYPSDRAVVAAWVTGLGIGAGTLETYVLLDRLNRAICGQLEY
                     GMREEPGVQSPAETLRLGTGSCRDYATLFIEACRGLGLASRFVSGYLHTSETAAGHAS
                     THAWAEVYLPGPGWKGFDPTGGVLTGSDHIPVAVARHPEAIPPVSGS"
     gene            complement(835..>2034)
                     /locus_tag="PLQ18_11940"
     CDS             complement(835..>2034)
                     /locus_tag="PLQ18_11940"
                     /inference="COORDINATES: protein motif:HMM:NF013109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HNE02407.1"
                     /translation="MSGAQSSSAFSAVSAAFGLGALLLLLWLRRHRPHWPGVLLVCGL
                     GIVLSHFSAYAGHGGAVIGSVPEALPQLSLPPALDFGTHRALLAPALVVALVSFTEAM
                     SSARTLARRRGERWNENQELIGQGLAKIASAASGAFPVSGSFSRSALNEYAGARTAWS
                     TLFAAACVFAFLLAGTAVLAPLPQAVLAAVVIVPVTGLLNVAGLRRIGRLARDDGLVA
                     LITFAATLFAVPQLHWGVLAGLIAGLAASMLRRSQPRIVELGVLPDGRLRARERYGLP
                     RPAPDLIVLRPDTALVYASAAAVERAVLDHVDAGVQRVLLVGTAINDLDVTGLDTLRE
                     LRRTLAARGTTLYLCRMKLPVWRLLERAGVPAEFGENRCFRTERQAIAALTGENPAPA
                     AADRPHP"
BASE COUNT          312 a          729 c          752 g          241 t
ORIGIN      
        1 aaactgcctg acaccggcgg gatcgcctcc gggtggcggg cgaccgcaac cgggatgtgg
       61 tcgctgccgg tcagcacgcc gccggtcggg tcgaagccct tccagcccgg ccccggcagg
      121 tagacctcgg cccaggcgtg ggtcgaggca tggccggcgg cggtctcgga ggtgtgcagg
      181 taaccgctga cgaagcggct cgccagcccg aggccgcggc aggcctcgat gaacagcgtc
      241 gcgtagtcgc ggcaggagcc ggtgccgagg cgcagcgttt cggccggcga ctgcacgccg
      301 ggctcctcgc gcatgccgta ttccagctgc ccgcagatgg cgcggttcag ccggtcgagc
      361 agcacgtagg tttccagcgt ccccgccccg atgccgagtc cggtgaccca ggccgccacc
      421 acggcgcgat cggacggata gaccggcgtg ctcgccagct gcaggtcggt gcgttcggcc
      481 gctgcgtact cgaacgggta gtgcagcgcg tagtcctcga ccagaaagtc gagcggcgca
      541 tcgtcgtggt gttcgatgac cacttcgctg tcgatgcaga gccgtccggc cggctgcggg
      601 aagtgcgcga tcgcgaccga gttgtcatcg gcatcgcggc accagcgcag ctgcgcggcc
      661 ggggtgatgt cgaggcgcga gcgttcgacg cgcacctcgt ggccctcgcg cgggcgcagc
      721 agcagccggt gcgggccgag ccggaccggc tcgggatagc ggtattcggt gcggtgcgcg
      781 atcaggatgc gcttcatcgg cggctctccg gcggcgatgc ggacctgcac cgtactacgg
      841 atgcgggcga tcggccgcag ccggcgcagg gttttcgccg gtcagcgccg cgatcgcctg
      901 acgctcggtg cggaaacagc ggttctcgcc gaactccgcc ggcacgccgg cgcgctccag
      961 cagccgccag accggcagct tcatgcggca gagatagagc gtcgtgccgc gtgccgccag
     1021 cgtgcggcgc agctcgcgca aggtatcgag gccggtgaca tcgagatcat tgatcgccgt
     1081 gccgaccagc agcacgcgct gcacgccggc atcgacgtga tcgagcaccg cgcgctcgac
     1141 cgccgccgca ctggcgtaga ccagcgccgt gtccgggcgc agcacgatca ggtccggcgc
     1201 cggccgcggc aggccgtagc gctcgcgcgc gcgcagccgg ccgtcgggca gcacgccgag
     1261 ttcgacgatg cgcggctggc tgcggcgcag catcgaggcc gcgaggccgg cgatcaggcc
     1321 ggcgagcacg ccccagtgca gctgcggcac cgcgaacagc gtcgccgcga aggtgatcag
     1381 cgccaccagg ccgtcgtcgc gcgccagccg cccgatgcgc cgcaggccgg ccacgttgag
     1441 cagcccggtc accggcacga tgacgacggc cgccagcacc gcctgcggca gcggcgcgag
     1501 cacggcggtg ccggcgagca ggaacgcgaa cacgcaggcg gcggcgaaca gcgtcgacca
     1561 ggccgtgcgg gcgccggcgt attcattgag ggccgagcgc gagaacgagc cgctgaccgg
     1621 aaacgccccg ctggcggcgc tcgcgatctt ggcgagaccc tgaccgatca gctcctgatt
     1681 ctcgttccag cgctcgccgc gccggcgtgc cagcgtgcgc gcactcgaca tcgcctcggt
     1741 gaagctgacc agcgccacga ccagcgccgg cgcgagcagc gcccggtgcg tcccgaaatc
     1801 gagcgccggt ggcagcgaca gctgcggcag cgcctcgggc acgctgccga tcacggcccc
     1861 gccatggcct gcgtaagcgc tgaagtggct gagcacgatg ccgagcccgc agacgagcag
     1921 cacgcccggc cagtgcggcc ggtgccggcg cagccacagc agcagcagca gggccccgag
     1981 cccgaaggcc gcggacacgg ccgaaaaggc cgacgaagac tgagcccctg acat
//
LOCUS       DAOQJH010000577         2031 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_44703_length_2031_cov_3.405364, whole genome shotgun sequence.
ACCESSION   DAOQJH010000577 DAOQJH010000000
VERSION     DAOQJH010000577.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44703_length_2031_cov_3.405364"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..554)
                     /locus_tag="PLQ18_11945"
     CDS             complement(<1..554)
                     /locus_tag="PLQ18_11945"
                     /inference="COORDINATES: protein motif:HMM:NF024991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNE02408.1"
                     /translation="MDEPHPIPRAGHWYVTPVLARTTEALERLAHTGAFIGVVKGPPG
                     AGKSLLLERLAGVLAQDEQLAIAHLMAGAGKGGLLVGLAVAFGVPTDGNPTQTRRALI
                     QHLTGLHGSGRCPVVLIDAAEHLSSLELSLLIRLGLSADKGKSGLMNVILAGSGDLDQ
                     RIAVAGEHLSRQRVYVAALPALTRA"
     gene            complement(582..1661)
                     /gene="aroB"
                     /locus_tag="PLQ18_11950"
     CDS             complement(582..1661)
                     /gene="aroB"
                     /locus_tag="PLQ18_11950"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014779202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HNE02409.1"
                     /translation="MQTLTVDLGDRSYPILIGPGLLRQPEAWRSCIPGRQVLVVSNET
                     VAPLYLEATLLALEGLQVETVVLPDGEQYKTLAVLEQVFDRLLRARFDRGCTLLALGG
                     GVIGDMTGFAAACYQRGVSFVQVPTTLLAQVDSSVGGKTGVNHALGKNMIGAFHQPRV
                     VIADTDTLNTLPDRELAAGLAEVIKYGLIRDLPFLDWIEAHLDALLAREPQALAQAIQ
                     RSCQDKADIVGRDERESGERALLNLGHTFGHAIETGSGYGNWLHGEAVAAGTMMALDL
                     SARLGWLGAAELGRVQRLFERARLPVAPPAGLGADDFLNLMAVDKKASEGRIRLVLLE
                     GLGNAVVRGDTPPALLRATLDACCR"
     gene            complement(1736..>2031)
                     /locus_tag="PLQ18_11955"
     CDS             complement(1736..>2031)
                     /locus_tag="PLQ18_11955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HNE02410.1"
                     /translation="IVLATGGGAVLAAENRACLSTRGFVVYLETSVDEQLRRTARDSN
                     RPLLQTADPRARLTELLQTRDPLYREIADFVVHTDGRQSRRVAALILDHYERG"
BASE COUNT          300 a          713 c          719 g          299 t
ORIGIN      
        1 gcccgcgtca gcgccggcag tgcggccacg tagacgcgct ggcgcgacag atgctcgccg
       61 gcgacggcaa tgcgctgatc gagatcgccg ctgccggcca ggatcacgtt catcaggccg
      121 ctcttgccct tgtccgccga cagcccgagc cggatcagca gcgacagttc cagcgaggac
      181 aggtgctctg ccgcgtcgat cagcacgacc gggcagcgtc cgctgccgtg caggccggtc
      241 aggtgctgga tcagcgcgcg acgggtctgg gtcggattgc cgtcggtcgg cacgccgaag
      301 gccacggcca gtccgacgag cagcccgccc ttgccggcac cggccatcag gtgcgcgatg
      361 gcaagctgtt cgtcctgcgc gagcactccg gccagccgtt cgagcagcag gctcttgccg
      421 gcgccgggcg gccccttgac gacgccgatg aacgcccccg tgtgggccag gcgttcgagt
      481 gcttcggtcg tgcgggccag taccggtgtg acgtaccagt gaccggcccg gggaatcgga
      541 tgcggctcgt ccatgcgcag tgcctggggt ccgggccgcg ctcagcggca gcaggcgtcg
      601 agcgtggcgc gcagcagcgc cggtggcgta tcgccgcgca cgaccgcgtt gccgagccct
      661 tcgagcagca ccagccggat gcggccctcg ctggccttct tgtcgacggc catcaggttc
      721 aggaagtcgt ccgccccgag gccggccggc ggcgcgaccg gcagccgtgc gcgttcgaac
      781 aggcgctgca cgcgcccgag ctcggctgcg cccagccagc cgagccgcgc cgacaggtcg
      841 agcgccatca tcgtgccggc ggccacggct tcgccgtgca gccagttgcc gtagccgctg
      901 ccggtttcga tcgcgtgtcc gaaggtgtgg ccgaggttca gcagcgcgcg ctcgccgctc
      961 tcgcgttcat cccgcccgac gatgtcggcc ttgtcctgac agctgcgctg gatcgcctgc
     1021 gccagcgcct gcggctcgcg cgccagcagc gcatcgaggt gggcttcgat ccagtcgaga
     1081 aagggcaggt cgcggatcag cccgtacttg atgacctcgg ccagaccggc agccagttcg
     1141 cgatccggca gcgtgttcag cgtgtcggtg tcggcgatca ccacgcgcgg ctggtgaaag
     1201 gcgccgatca tgttcttgcc gagcgcgtgg ttgacgccgg tcttgccgcc gaccgacgaa
     1261 tcgacctgcg ccagcagcgt cgtcggcacc tgtacgaatg acacgccgcg ctggtaacag
     1321 gccgcggcga agccggtcat gtcgccgatc acgccaccgc cgagcgcgag cagcgtgcaa
     1381 ccccgatcaa agcgcgcgcg cagcagccgg tcgaacacct gttcgagcac cgcgagcgtc
     1441 ttgtactgct cgccatccgg cagcacgacg gtctcgacct gcagaccttc gagcgccagc
     1501 agcgtggctt cgagatacag cggcgccacc gtctcgttgc tgaccaccag cacctgccgg
     1561 cccgggatgc aggagcgcca ggcctcgggc tgccggagca ggccggggcc gatcaggatc
     1621 gggtagctgc ggtcgccgag atcgacggtc agggtctgca tgggtgcgac ggacatggcg
     1681 ggaaacgggt cgctaggttg cgggaagctc ccggagcctg caagcagcgg ccaccttagc
     1741 cgcgctcgta gtgatcaagg atcagtgccg cgacccgccg ggactggcgg ccgtcggtat
     1801 gcacgacgaa gtcggcgatc tcccggtaca gcggatcccg ggtctgcagc agttccgtca
     1861 gccgcgcgcg cgggtcggcg gtctgcagca gcgggcgatt gctgtcccgc gcagtgcgcc
     1921 gcaactgttc gtccaccgag gtttcgagat agacgacgaa gccgcgcgtg ctcaggcagg
     1981 cacggttttc cgccgccaga acggcccccc cgccggtggc gagcacgatg c
//
LOCUS       DAOQJH010000578         2030 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_44730_length_2030_cov_4.267342, whole genome shotgun sequence.
ACCESSION   DAOQJH010000578 DAOQJH010000000
VERSION     DAOQJH010000578.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2030
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44730_length_2030_cov_4.267342"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            171..1502
                     /locus_tag="PLQ18_11960"
     CDS             171..1502
                     /locus_tag="PLQ18_11960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012633066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter"
                     /protein_id="HNE02411.1"
                     /translation="MWTFWLEFILVLGAILLGIRRGGVALGLIGGLGVAVLVFVFRTP
                     PSEPPVTVMLIILAVVTASATLQVAGGLDWLVQQTERLLRAYPQRVTLLAPLATFLLT
                     VCVGTGHAVYALLPVIGDVALRTGIRPERPMAISSVASQMGITASPVAAAVTTFLAFA
                     AKTPTPVGLFDIVMITLPAGLAGVLVAALWSMKRGKDLADDPEYQARLADPQFRAALD
                     VSVTTLGTELPKTAKLSVALFFGGVLTIVMLALFPVLVPVFGGKAVPMTTIVQIVMLG
                     FGALILFATGVKAPDIARSSVFTAGMIAVVSIFGIAWMSDTFVSGNKAFLIEQIEAMV
                     KFAPWTFALAMFAVSAFVKSQAATLTIMIPFGLALSLPVPLLLGLMPSSYAYFFFAFY
                     PSDLAAINMDRTGTTHIGKYLLNHSFMIPGLIGVGVSTTVAYLLSRVLFSY"
     gene            complement(1569..>2030)
                     /locus_tag="PLQ18_11965"
     CDS             complement(1569..>2030)
                     /locus_tag="PLQ18_11965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004366206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase"
                     /protein_id="HNE02412.1"
                     /translation="GDYTRLLPGRLHVGSVVTLEGPYGRFTFEGDARRQIWIGGGIGI
                     TPFLARLQALGSAPDGRDIDLFHSTAQYDPDVIARLQREATAAKVRLHVLWDPRDGRL
                     DVAGIVSQVPDWREADVWFCGPGGFGRAIRDGLLRLGLPAGRFHQELFEMR"
BASE COUNT          270 a          713 c          682 g          365 t
ORIGIN      
        1 cgctgctgca tcgcatcaga acccgacctg cgtcatgtcc gtgactgcac acggcgccca
       61 tgctgcgcag caaccgccgg tcctcgcgcc gccctcccgc gccgggaccg gtggcagtcg
      121 tccggcgcga gccggtgcgt ggtcttgccc gtccccccgg gagaagtgcg atgtggacct
      181 tctggcttga attcatcctg gtgctcggcg ccatcctgct cggcatccgc cgcggcggcg
      241 tggcgctcgg gctgatcggc ggcctcggtg tcgccgtgct ggtgttcgtg ttccgcacgc
      301 cgccgtcgga accgccggtc accgtgatgc tgatcatcct cgccgtggtc acggcctcgg
      361 cgacgctgca ggtcgccggc ggtctcgact ggctggtgca gcagaccgaa aggctgctgc
      421 gcgcctaccc gcagcgcgtg acgctgctcg cgccgcttgc gaccttcctg ctgacggtgt
      481 gcgtcggcac cggccacgcc gtctatgcgc tgctgccggt gatcggcgac gtggccctgc
      541 gcaccggcat ccgcccggag cggccgatgg cgatctccag cgtcgcctcg cagatgggca
      601 tcaccgccag cccggtcgcc gcggccgtga ccaccttcct cgccttcgca gcgaagacgc
      661 cgacgccggt cgggctgttc gacatcgtga tgatcacgct gccggccggg ctcgccgggg
      721 tgctggtggc ggccctctgg agcatgaagc gcggcaagga cctcgccgat gatcccgaat
      781 atcaggcccg gctcgccgac ccgcagtttc gcgccgcact cgacgtgtcg gtgacgacgc
      841 tcggcaccga gctgccgaag accgcgaagc tgtcggtggc gctgttcttc ggcggcgtgc
      901 tcacgatcgt catgctggcg ctctttccgg tgctggtgcc ggtgttcggc ggcaaggccg
      961 tgccgatgac gaccatcgtg cagatcgtca tgctcggctt cggcgcgctg atcctgttcg
     1021 cgaccggcgt caaggcgccg gacatcgccc gctccagcgt gttcacggcc ggcatgatcg
     1081 cggtcgtgtc gatcttcggc atcgcgtgga tgagcgacac cttcgtcagc ggcaacaagg
     1141 ccttcctgat cgagcagatc gaagcgatgg tcaagttcgc gccgtggacg tttgcgctgg
     1201 cgatgttcgc ggtgtcggct ttcgtgaaaa gccaggccgc gacgctgacg atcatgattc
     1261 cgttcggact ggccctgtcg ctgcccgtgc cgctgctgct cgggctgatg ccgtcgagtt
     1321 atgcgtattt cttcttcgcg ttctatccga gcgatctggc cgcgatcaac atggaccgca
     1381 ccggcaccac gcacatcggc aagtacctgc tcaatcacag cttcatgatt cccggcctga
     1441 tcggcgtcgg cgtttcgacg acggtcgcgt atctgctgtc gcgggtgttg ttctcctatt
     1501 gagccgtcac accggcaatc aagccggagg cgggacgcag cgcctctgcg tcccgtcctg
     1561 ccggagcgtc agcgcatttc aaacaattcc tgatgaaagc gcccggccgg cagtcccagc
     1621 cgcagcaggc catcgcggat cgcgcgcccg aatccgcccg gcccgcagaa ccagacgtcg
     1681 gcctcgcgcc agtccggcac ctggctgacg atgccggcga catcgagccg gccgtcgcgc
     1741 gggtcccaga gcacgtgcag gcgcaccttc gcggccgtcg cctcgcgctg cagccgggcg
     1801 atcacgtccg gatcgtattg cgcggtgctg tggaagagat cgatgtcgcg gccgtccggc
     1861 gcgctgccga gtgcctgcag gcgggcgagg aacggcgtga tgccgatgcc gccgccgatc
     1921 cagatctgcc ggcgggcgtc gccttcgaac gtgaagcgtc cgtaggggcc ttcgagcgtc
     1981 acgacgctgc cgacgtgcag ccggcccggc agcaggcgcg tgtagtcgcc
//
LOCUS       DAOQJH010000579         2029 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_44777_length_2029_cov_3.128673, whole genome shotgun sequence.
ACCESSION   DAOQJH010000579 DAOQJH010000000
VERSION     DAOQJH010000579.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44777_length_2029_cov_3.128673"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            13..354
                     /gene="hypA"
                     /locus_tag="PLQ18_11970"
     CDS             13..354
                     /gene="hypA"
                     /locus_tag="PLQ18_11970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012977320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HNE02413.1"
                     /translation="MHELSLCQSVVRSIEAAAGPHGFSRVRRVRLAIGAFAGVEPDAM
                     RFGFDVATQGTLAEGATLEILEVPGTAWCFDCNDTVTLAQRFDACPRCAGFRLRLTGG
                     EELRIQDLEVD"
     gene            358..1245
                     /gene="hypB"
                     /locus_tag="PLQ18_11975"
     CDS             358..1245
                     /gene="hypB"
                     /locus_tag="PLQ18_11975"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004853525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HNE02414.1"
                     /translation="MCTVCGCGAGELRIDGLAPLAPAAAAPLAVEGLRLRMAGDLSKQ
                     GQPSPSPAAPASKQDLHFGHGPAGVHVGGVEQGRLVQLEIDILAKNNDFAARNRRWFA
                     AHGVQALNLVSSPGSGKTTLLCATIRRLAGERALAVIEGDQQTANDAERIRATGAPAV
                     QINTGKGCHLDADMIARALPRLPLDPGTLLFIENVGNLVCPAAFDLGEAAKVAILSVT
                     EGEDKPLKYPDIFAAASLVLLNKSDLLPHLDFDLEYALDCLRRVNPRAPVLSVSAKTG
                     EGLDAWLAWLRTPAPVAEG"
     gene            1258..>2029
                     /locus_tag="PLQ18_11980"
     CDS             1258..>2029
                     /locus_tag="PLQ18_11980"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HNE02415.1"
                     /translation="MNIPAPVAVPITRLALQVRGLVQGVGLRPWIWRLAHEHGLAGWV
                     RNDGDGVAIEVQGPAPALALFRAALAVPPPLARLDAIEAQTLAPLAGSDGFAILDSGR
                     GPVRTGIGADTAVCADCLAETFDPAGRRWRYPFTTCTHCGPRYTIAARLPWDRPNTAL
                     AGFALCASCREEYENPADRRFHAEALACPACGPRLRLQRPEGSRAEVDGDPLAATLAL
                     FRAGGIVAVEGLGGFHLMCDARNAATVAGLRRRKRRPGK"
BASE COUNT          274 a          738 c          717 g          300 t
ORIGIN      
        1 ggatgaccgc cgatgcatga gctatcgctg tgccagagcg tggtgcgctc gatcgaggcc
       61 gcggccggcc cgcacggttt ctcgcgcgtg cgccgcgtgc ggctggcgat cggcgccttc
      121 gccggcgtcg agccggatgc gatgcgcttc ggcttcgatg tcgccacgca gggcacgctc
      181 gccgaaggcg cgacgctcga aatcctcgaa gtgccgggca cggcctggtg ctttgactgc
      241 aatgacaccg tgacgctcgc gcagcgtttc gatgcctgcc cgcgctgcgc cggcttccgg
      301 ctgcggctga ccggcggcga ggaactgcgc atccaggatc tggaggtcga ctgaagcatg
      361 tgtacggtct gcggctgtgg cgcgggtgaa ctgcgcatcg acggactggc cccgctcgcc
      421 ccggctgcgg ccgcaccgct tgcggtcgag ggcctgagac tgcgcatggc tggcgatctt
      481 tcgaaacaag gccagccgtc gccgtcgccg gccgcgccgg cctcgaaaca agacctgcac
      541 ttcggccacg gcccggccgg cgtgcacgtc ggcggcgtcg aacagggccg gctcgtgcag
      601 ctcgaaatcg acatcctcgc caagaacaac gacttcgcgg cgcgaaaccg ccgctggttc
      661 gcggcgcacg gcgtgcaggc gctgaacctg gtctccagcc ccggctcggg caagaccacg
      721 ctgctctgcg cgaccatccg ccgccttgcc ggcgaacgtg cgctcgccgt catcgaaggc
      781 gaccagcaga ccgccaacga cgccgagcgc atccgcgcca ccggcgcgcc ggccgtgcag
      841 atcaacaccg gcaagggctg ccacctcgat gccgacatga tcgcgcgcgc gctgccgcgg
      901 ctgccgctcg atccgggcac gctgctgttc atcgagaacg tcggcaacct ggtctgcccg
      961 gcggccttcg atctcggcga ggcggcgaag gtcgcgatcc tgtcggtcac cgagggcgag
     1021 gacaagccgc tgaaatatcc ggacatcttc gcagcggcga gcctcgtgct gctgaacaag
     1081 tccgatctgc tgccgcacct cgacttcgat ctcgaatacg cgctcgactg cctgcggcgc
     1141 gtcaatccgc gcgcgccggt gctgagcgta tcggcgaaga ccggcgaagg gctcgacgcc
     1201 tggctggcgt ggctgcgcac gccggcgccg gtcgcggagg gctgaagcgg cccgctcatg
     1261 aacatccccg ctcccgtcgc cgtgccgatc acccggctcg cgctgcaggt gcgcggtctg
     1321 gtgcagggcg tcggcttgcg gccgtggatc tggcggctcg cacacgagca cggcctcgcc
     1381 ggctgggtgc gcaacgacgg cgacggcgtc gcgatcgagg tgcagggacc ggcgccggcg
     1441 ctggccttgt ttcgtgccgc gctcgccgtg ccgccgccgc tcgcgcggct cgacgcgatc
     1501 gaggcgcaga cgctggcacc gctcgccggc tccgatggct tcgcgatcct cgacagcggc
     1561 cgcgggccgg tgcgcaccgg catcggcgcc gacaccgcgg tctgcgcgga ctgcctcgcc
     1621 gagaccttcg atccggccgg aaggcgctgg cgctatccgt tcacgacctg cacgcactgc
     1681 ggcccgcgct acacgatcgc cgcgagactc ccttgggatc gcccgaacac cgcgctcgcc
     1741 ggctttgcgc tctgcgcaag ctgccgtgag gaatacgaaa atccggccga ccggcgcttt
     1801 cacgccgagg cgctggcctg cccggcctgc gggccgcggc tgcggctgca gcggcccgag
     1861 ggcagccgcg cggaggtcga tggcgatccg ctcgccgcga cgctggcctt gtttcgtgcc
     1921 ggcggcatcg tcgcggtcga gggcctcggc ggttttcatc tgatgtgcga tgcgcgtaat
     1981 gccgccacgg tcgccgggct gcgccggcgc aagcgcaggc ccggcaaac
//
LOCUS       DAOQJH010000580         2023 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_45029_length_2023_cov_4.350610, whole genome shotgun sequence.
ACCESSION   DAOQJH010000580 DAOQJH010000000
VERSION     DAOQJH010000580.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2023
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45029_length_2023_cov_4.350610"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1099)
                     /gene="dnaX"
                     /locus_tag="PLQ18_11985"
     CDS             complement(<1..1099)
                     /gene="dnaX"
                     /locus_tag="PLQ18_11985"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018953831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HNE02416.1"
                     /translation="MSHQVLARKWRPKRFAELVGQEHVVRALGNALANGRLHHAFLFT
                     GTRGVGKTTIARILAKSLNCETGITAEPCGVCAACREIDEGRFVDLIEVDAASRTKVE
                     DTRELLENVQYAPARGRYKVYLIDEVHMLSTSSFNALLKTLEEPPPHVKFLLATTDPQ
                     KLPVTVLSRCLQFSLKRLPAALIQSRLADILDAEGIAHEPAALAALARAGDGSMRDAL
                     SLLDQAIAFGAGRVGALDTAAMLGTIDRQHVVHLAEALAGGDAASVLAAIERLDREAP
                     DYDGVLAELLSLLQRVALAQAVPGANDELYDSDTLVRLAAAFTAEDVQLCYQIALHAR
                     RDLPLAPEPRGGFEMALLGELDCHINAIPFNP"
     gene            complement(1177..1273)
                     /gene="ffs"
                     /locus_tag="PLQ18_11990"
     ncRNA           complement(1177..1273)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PLQ18_11990"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            1480..>2023
                     /locus_tag="PLQ18_11995"
     CDS             1480..>2023
                     /locus_tag="PLQ18_11995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002928323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNE02417.1"
                     /translation="MKLETLAIHAGYSPDPTTKAVAVPIYQTTSYAFDDTQHGADLFD
                     LKVAGNIYTRIMNPTTAVLEARMAALEGGIGALGLASGQAAITYAIQTIAEAGDNIVA
                     TGTLYGGTYNLFAHTLPQYGIEVRFVDYRDPASAAALIDGRTKAVFCETIGNPLGNVV
                     DFEAFSQVAHAHGVPLIVDNT"
BASE COUNT          315 a          702 c          716 g          290 t
ORIGIN      
        1 ggggattgaa cgggatcgcg ttgatgtggc agtcgagctc gcccagcagc gccatctcga
       61 agccgccgcg cggctcgggc gcgagcggca gatcgcggcg cgcgtgcagc gcgatctggt
      121 agcagagctg cacgtcctcg gccgtgaacg cggcggcgag acggacgagc gtatcgctgt
      181 cgtacagctc gtcgttggcg ccgggcacgg cctgcgcgag cgcgacgcgc tgcagcagcg
      241 acagcagctc ggcgagcacg ccgtcgtaat ccggcgcctc gcgatcgagc cgctcgatcg
      301 ccgcgagcac actggccgca tcgccgccgg ccagggcttc ggcgaggtgc acgacgtgct
      361 ggcggtcgat cgtgccgagc atcgccgcgg tgtcgagggc gccgacgcga ccggcgccga
      421 aggcgatggc ctgatcgagc agcgacagcg catcgcgcat cgagccgtcg ccggcgcgcg
      481 cgagtgcggc gagcgcggcc ggttcgtgtg cgatgccttc ggcgtcgagg atgtcggcga
      541 gccggctctg gatcagcgcc gccggcagac gcttgaggct gaactgcagg cagcgcgaca
      601 gcacggtgac cggcagcttc tgcgggtcgg tggtcgcgag caggaacttc acgtgcggcg
      661 gcggctcttc gagcgtcttc agcagcgcgt tgaagctgct ggtgctcagc atgtgcactt
      721 cgtcgatcag gtagaccttg tagcgaccgc gcgccggtgc gtactggacg ttttcgagca
      781 gctcgcgcgt gtcctcgacc ttggtgcgcg aagccgcatc gacctcgatc agatcgacga
      841 agcggccctc gtcgatctcg cggcaggccg cacagacgcc gcagggctcg gcggtgatgc
      901 cggtctcgca gttcagcgac ttggcgagga tgcgcgcgat cgtggtcttg ccgacgccgc
      961 gcgtgccggt gaacaggaag gcgtgatgca ggcggccgtt ggcgagcgcg ttgccgagcg
     1021 cgcgcacgac gtgctcctgc ccgaccagtt ccgcgaagcg cttcggccgc catttgcgcg
     1081 ccagaacctg atggctcatc tcgtgccgcc tgccgggtgg ttcgcgtggg ccgccgcgcc
     1141 ctgccatgcc tgacgcggcc ggaaatgggt ggtggcccgt accagacgca ccccggcgcc
     1201 cgcatcggcc gctaccgttg ctcccttccg ggcctggcgg ggttcacggc ctagcgtcgc
     1261 gaggggaccg atacgggcca ccatagaaaa cgtgcagctc gaagactgcg ctcctgcgac
     1321 cggccggccg cgagggaggc gcagtctagc cgaagcgccg gccgcgggga aaccgccgca
     1381 ggcgcagtcc gggaaccagc ttctacgcta ggggccgggc ggtcgcagac cgggagcagc
     1441 ctcccgtccg cccgcaatcc catcacccga gtgcagacga tgaagctcga aaccctcgcg
     1501 atccacgccg gctacagccc cgacccgacc acgaaggcgg tcgcggtgcc gatctaccag
     1561 accacgtcgt atgcgttcga cgacacgcag cacggcgcgg acctgttcga cctgaaggtc
     1621 gccggcaaca tctacacgcg catcatgaac ccgaccacgg cggtgctgga ggcgcgcatg
     1681 gcggcgctcg aaggcggcat cggcgcgctc ggactcgcct ccggacaggc ggcgatcacc
     1741 tacgcgatcc agaccatcgc cgaggccggc gacaacatcg tcgccaccgg cacgctctac
     1801 ggcggcacct acaacctgtt cgcgcacacg ctgccgcagt acggcatcga ggtgcgcttc
     1861 gtcgattacc gcgatccggc ctcggcggcg gcgctgatcg acggccgcac caaggcggtg
     1921 ttctgcgaga cgatcggcaa tccgctcggc aacgtcgtcg acttcgaagc cttctcgcag
     1981 gttgcgcacg cgcacggcgt gccgctcatc gtcgacaaca cgg
//
LOCUS       DAOQJH010000581         2018 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_45254_length_2018_cov_4.159959, whole genome shotgun sequence.
ACCESSION   DAOQJH010000581 DAOQJH010000000
VERSION     DAOQJH010000581.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45254_length_2018_cov_4.159959"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2018
                     /locus_tag="PLQ18_12000"
     CDS             <1..>2018
                     /locus_tag="PLQ18_12000"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNE02418.1"
                     /translation="KLLLDPVDGRIVDVNPAACRRLGWSREELLTMGVTDIDPLVGLE
                     QLRAMREEVARKGHVRVESEHCTRDGERFPVEIVATGMPYGDGQYNCAIVRDISARRA
                     AETQLRLYAEVFEHAAESIMITDAGCSIVAVNPAFTTVTGYAPDEVLGRNPRMLASGR
                     QDAAFYRAFWQSLDAHGLWQGEIWNRRKSGEVFPEWLSVSVVRDADAGPQHYIAVFTD
                     LSEHKAHERRIQFLAQYDPLTRLPNRVLLFERLEHALAAARRGARRCALLFLDLDRFK
                     NVNDSLGHGIGDRLLEAVAQRLRAALRDCDTVARLGGDEFVVLLEDLPAGDGEREAAL
                     VAGKLLAALAQPFQIDTHELRITSSVGISLYPGHGADAETLIRSADTAMYHAKQSGRD
                     NFQFFTEDMNARVLERLTLENGLRRALAQQGFALVWQPQLAIGDGRLIGFEALLRWPQ
                     PEGRAQVPPAQFIPVAEDCGLIGAIGDWVLDAACRQLCAWRAAGLPGVRIAVNLSALQ
                     LRDARLADKVAAALAVCGLDGAALELEVTESALMSDPPQTAGTLARLRALGVQLAIDD
                     FGTGYSSLAYLKQFPIDRLKIDRSFVRDLASDPNDAAIARTIITLAHSLGLRVIAEGV
                     ETPAQLGFLRGHGCDEAQGYLYAPPLSPEQAAEVLAAGRIRPAASTSA"
BASE COUNT          288 a          709 c          723 g          298 t
ORIGIN      
        1 caagctgctg ctcgatcccg tcgacggccg gatcgtcgac gtgaatccgg ccgcctgccg
       61 ccggctcggc tggtcgcgcg aagagctgct gaccatgggc gtcaccgaca tcgacccgct
      121 ggtcgggctc gaacaactgc gtgcgatgcg cgaagaggtc gcgcgcaagg gccatgtccg
      181 cgtcgaatcc gagcactgca cgcgcgacgg cgaacgcttt ccggtcgaga tcgtcgcgac
      241 cgggatgccg tacggcgacg gccagtacaa ctgcgcgatc gtgcgcgaca tcagcgcccg
      301 gcgcgccgcc gaaacccagc tgcggctgta tgcggaggtg ttcgagcacg ccgccgagtc
      361 gatcatgatc accgacgccg gctgcagcat cgtcgccgtg aatccggcgt tcacgacggt
      421 gaccggctac gcgccggacg aggtgctcgg gcgcaatccg cgcatgctcg cctccgggcg
      481 ccaggatgca gccttctacc gggccttctg gcagtcgctc gatgcgcacg ggctgtggca
      541 gggcgagatc tggaaccggc gcaagtccgg cgaggtgttt ccggagtggc tgtcggtcag
      601 cgtcgtgcgc gatgccgatg ccggcccgca gcactacatc gcggtgttca ccgacctcag
      661 cgagcacaag gcgcatgagc ggcgcatcca gttcctcgcg cagtacgacc cgctgacgcg
      721 gctgccgaac cgggtgctgc tgttcgagcg gctcgaacac gcgctggcgg cggcgcggcg
      781 gggtgcccgg cgctgcgcgc tgctgtttct cgatctcgac cgcttcaaga acgtcaacga
      841 ttcgctcggc cacggcatcg gtgaccggct gctcgaagcc gtcgcgcagc gcctgcgcgc
      901 ggcgctgcgc gactgcgaca ccgttgcccg gctcggcggc gacgagttcg tcgtgctgct
      961 cgaagacctg ccggccggcg acggcgaacg cgaggcggcg ctcgtcgccg gcaagctgct
     1021 ggccgcgctc gcgcagccct tccagatcga cacgcacgag ctgcgcatca ccagcagcgt
     1081 cggcatcagc ctgtatccgg gccacggtgc cgatgccgag acgctgatcc gcagcgccga
     1141 cacggcgatg taccacgcca agcagtccgg ccgggacaac ttccagttct tcaccgagga
     1201 catgaacgcc cgcgtgctcg aacgcctgac gctggagaac ggcctgcgcc gggcgctggc
     1261 gcagcagggc ttcgcgctgg tctggcagcc gcagctcgcg atcggcgacg gccggctgat
     1321 cggcttcgag gcgctgctgc gctggccgca gccggagggg cgggcgcagg tgccgccggc
     1381 gcagttcatt ccggtggccg aggactgcgg actgatcggc gcgatcggcg actgggtgct
     1441 cgatgccgcc tgccggcagc tctgcgcgtg gcgggccgcc ggcctgccgg gggtgcgcat
     1501 tgccgtgaac ctctcggcgc tgcagctgcg cgatgcccgg ctggccgaca aggtggccgc
     1561 ggccctcgcc gtctgcgggc tggacggtgc cgcgctggag ctcgaagtca ccgagagcgc
     1621 gctgatgagc gatccgccgc agaccgccgg cacgctggca cggctgcgcg cgctcggcgt
     1681 gcagctcgcg atcgatgact tcggcaccgg gtattcgtcg ctcgcgtacc tgaagcagtt
     1741 tccgatcgac cgcctgaaga tcgatcgcag cttcgtgcgt gatctcgcca gcgatccgaa
     1801 cgatgcagcc atcgcgcgca cgatcatcac gctcgcgcac agtctgggcc tgcgcgtgat
     1861 cgccgagggg gtcgagacgc cggcgcagct cggattcctc cgcggccacg gctgcgacga
     1921 ggcgcagggc tatctgtacg cgccgccgct cagtccggag caggctgccg aggtgctcgc
     1981 cgccggccgg atccggccgg cggcgagcac ctcggcag
//
LOCUS       DAOQJH010000582         2014 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_45425_length_2014_cov_3.692700, whole genome shotgun sequence.
ACCESSION   DAOQJH010000582 DAOQJH010000000
VERSION     DAOQJH010000582.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45425_length_2014_cov_3.692700"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..422
                     /locus_tag="PLQ18_12005"
     CDS             <1..422
                     /locus_tag="PLQ18_12005"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HNE02419.1"
                     /translation="VDADDHPVGMTINSFGSLSLGPPLILWSIGNGSSLRPAFEKHGY
                     FSINVLRVDQVALSNTFAMSPGDRFAGLDWTAGLGGVPLLNDCLARFQCAVERVLPGG
                     DHQLILGRVVALETFDGEPLLFYAGGYRRIGDSAGGW"
     gene            complement(438..806)
                     /locus_tag="PLQ18_12010"
     CDS             complement(438..806)
                     /locus_tag="PLQ18_12010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015258635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HNE02420.1"
                     /translation="MSAATLLTLYGIPNCDTVRRARRRLAELGIGHRFHDFRRDGLPP
                     ALLDGWIAVLGTAALLNTRGTTWRALPEDLRRQLDDGLARTLMLEQPALIRRPLLVRA
                     DGALRLGWDENGLADWLAGA"
     gene            complement(922..1209)
                     /locus_tag="PLQ18_12015"
     CDS             complement(922..1209)
                     /locus_tag="PLQ18_12015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02421.1"
                     /translation="MAGPQLPGQDGLPPSLFRTEARTVQVQPQACPHLSWSTMLTELR
                     AARLRGASASERLMMLDRWRRERCENCTITCLYPMPELPSALREQYEWRPR"
     gene            1506..>2014
                     /locus_tag="PLQ18_12020"
     CDS             1506..>2014
                     /locus_tag="PLQ18_12020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011059676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNE02422.1"
                     /translation="MSFPSTAPGTRAPLIGVALTLLAVLCFAVLDSSAKYLSQHYPVA
                     MVVWARYAVHTLLMLIAYAPARGRTLLHTRRPGRQLLRALLLLSVTILFVSGLRFLPL
                     AEATAIIYLTPLLVTALSVPLLGERVARGTWIAILTGLAGVLIVVRPGGALLGWTVLL
                     PLAAALCNSL"
BASE COUNT          262 a          704 c          721 g          327 t
ORIGIN      
        1 ccgtcgatgc cgacgatcac ccggtcggca tgacgatcaa ctccttcggc tcgctgtcgc
       61 tgggaccgcc gctgatcctc tggagcatcg gcaacggcag ttcgctgcgt ccggccttcg
      121 agaagcacgg ctacttcagc atcaacgtgc tgcgcgtcga tcaggtggcg ctgtcgaaca
      181 ccttcgcgat gtcgccgggg gatcgcttcg ccgggctcga ctggacggca ggcctgggcg
      241 gcgtgccgct gctcaacgac tgcctggcgc gcttccagtg cgcggtcgag cgcgtgctgc
      301 cgggggggga tcaccagctg atcctcgggc gtgtggtggc gctcgaaacc ttcgacggcg
      361 agccgctgct gttctacgcc ggcggctacc ggcgcatcgg cgatagcgcc ggcggctggt
      421 gattgcgggc tggggactca ggcgccggcc agccagtcgg ccagcccgtt ttcgtcccag
      481 ccgaggcgca gtgcgccatc ggcacgcacg agcagcggcc ggcggatcag cgccggctgt
      541 tcgagcatca gcgtgcgggc gaggccgtcg tcgagctgac ggcgcaggtc ttcgggcagg
      601 gcgcgccagg tcgtgccgcg ggtgttgagc agggcggccg tgccgagcac cgcgatccag
      661 ccatcgagca gtgccggcgg caggccgtcg cgccggaagt catggaagcg gtgcccgatg
      721 cccagctccg cgagccggcg ccgggcgcgg cgcaccgtgt cgcagttcgg gatgccgtag
      781 agcgtcagca gggtggcggc agacatcgcg tcggaaaccg gttggcaagg cagggcgggc
      841 ggcggtgcag gaggccggcc ggcgatgcgc ctgcagggcc tgttttcacc cgcccggccg
      901 gtgcgggcgc gcggcgcttc ctcagcgcgg gcgccactcg tactgttcgc gcagcgccga
      961 tggcagctcg ggcatcggat acaggcaggt gatcgtgcag ttttcgcagc gctcgcggcg
     1021 ccagcgatcg agcatcatca ggcgttccga cgccgatgcg ccacgcaggc gcgcagcacg
     1081 caactcggtg agcatcgtgc tccaggacag atgcggacag gcttgcggct gtacctgcac
     1141 ggttcgagcc tcggttcgaa acagggaagg gggcaaccca tcctgccccg gaagctgcgg
     1201 gccggccatc cgggcggctc ggaatccggc ctgagaaatt tctcagctgc acggggcgat
     1261 gaagattcgt ccacctgcac cccgttcgcg cagcttctcc tacgctttgc tggcaaccga
     1321 tccggttgtc acccccgtct ggaaagcact ggcgacaggt tcccaccccc gcggacctgt
     1381 cgccgatttt ttttgcacgt ttccggcgtg cccgatgcac ggctgcacgg ctgccgtacc
     1441 ggcgcggcag ccccgcaagc cagcggctac gatgcgcagc cccgcgccgc cgtaccggat
     1501 cgatgatgag cttcccgtcc accgcccccg gcacgcgagc gccgctgatc ggcgtcgccc
     1561 tcacgctgct cgccgtgctg tgcttcgccg tgctcgacag cagcgccaaa tacctctcgc
     1621 agcactatcc ggtggcgatg gtggtctggg cgcgctacgc ggtgcatacg ctgctgatgc
     1681 tgatcgccta tgccccggcg cgcggacgca cgctgctgca cacgcgccgg cccgggcgcc
     1741 agctgctgcg ggcgctgctg ctgctgtcgg tgaccatcct gttcgtcagc ggcctgcgct
     1801 tcctgccgct cgccgaggcg accgcgatca tctacctgac gccgctgctg gtgacggcgc
     1861 tgtcggtgcc gctgctcggc gagcgcgtcg cccgcggcac ctggatcgcg atcctgaccg
     1921 ggctggccgg cgtgctgatc gtcgtgcgcc cgggcggggc gctgctcggc tggacggtgc
     1981 tgctgccgct ggcggcggcg ctctgcaaca gcct
//
LOCUS       DAOQJH010000583         2006 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_45780_length_2006_cov_3.747309, whole genome shotgun sequence.
ACCESSION   DAOQJH010000583 DAOQJH010000000
VERSION     DAOQJH010000583.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2006)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2006)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2006
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45780_length_2006_cov_3.747309"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2006
                     /locus_tag="PLQ18_12025"
     CDS             <1..>2006
                     /locus_tag="PLQ18_12025"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HNE02423.1"
                     /translation="ELNAKIFATDIDPSATEFARRGIYPEPAVQGLEPELRERWFRQE
                     GRHYLISKTIRDLCVFARQDLVRDPPFLRMDLISCRNLLIYLKPNLQERLLNQFHYSL
                     TPGGYLFLGKSENITQSGRLFSTVDGRYKLFRRKSVLTPRPLMIGSGNLGIGQPASTG
                     NRSVPSRQARLEAMRDELLDRYAPPSILLGPGLEPMHFCGDARRFLRLPDGPADFGLL
                     SLLLPEMRGEFRTLQHRLQSADGSDHVSGHMVPVHTTLGSEYVRMRLSRLTVPEASED
                     MMLLSIEPMRATEPPPAAVSTGAAPAGSDHDHAVLTERLRDAENELGATREHLQAVIE
                     ELETSNEELQSLNEELQASTEELQSSNEELETTNEELQATNEELTTVNDELEAKSEEL
                     TQVNTQLVNIQQSVALGLIVVDADLRVMRYNPPAVRVFGLMNDDLGQSLLAVPSFVEI
                     GDLRRKLLDVVGSGRTLSEELRREEATFLMQIAPYREDSGVISGAVITLSDITRFREL
                     ERAHAEGDQRLRLIADTLQDAIWLATPGFTRMLYVSRGYFALWERTPESLYADPHSFL
                     DAVHFEDRARVRRQLLETDPPATDLTYRLLVPGAGQRWVRQCTSLVRCEESGPALYLA
                     TSVRDVTAAAAERERLQHSEMNLRTAIDLLPIAALLRDAEGRVVV"
BASE COUNT          331 a          730 c          668 g          277 t
ORIGIN      
        1 gaactcaacg ccaagatctt cgcgaccgac attgacccct cggcgaccga gttcgcgcgc
       61 cgcggcatct atccggagcc ggccgtgcag gggctcgaac cggagctgcg cgagcgctgg
      121 ttccgccagg aaggccggca ctacctgatc agcaagacga tccgcgacct gtgcgtgttc
      181 gcccgccagg atctggtccg cgacccgccg tttctgcgca tggacctgat cagctgccgc
      241 aacctgctga tctacctgaa gccgaacctg caggagcggc tgctgaacca gttccactac
      301 tcgctgacgc cggggggcta cctgttcctc ggcaagtccg agaacatcac gcagagcggc
      361 cggctgttct cgaccgtcga cggccgctac aagctgttcc ggcgcaagtc cgtgctgacg
      421 ccgcgcccgc tgatgatcgg cagcggcaac ctcggcatcg gccagccggc cagcaccggc
      481 aaccgcagcg tgccctcgcg ccaggcgcgg ctggaggcga tgcgcgacga gctgctcgat
      541 cgctacgcgc cgccgagcat cctgctcggc cccgggctgg agccgatgca cttctgcggc
      601 gatgcccggc gcttcctgcg cctgccggac ggcccggccg acttcggcct gctgtcgctg
      661 ctgctgccgg agatgcgcgg cgagttccgc acgctgcagc accggctgca gtccgccgac
      721 ggcagcgacc acgtcagcgg ccacatggtg ccggtgcaca cgacgctcgg ctccgagtac
      781 gtgcgcatgc gcctgagccg gctgaccgtg cccgaggcca gcgaggacat gatgctgctc
      841 agcatcgagc cgatgcgcgc gaccgagccg ccgccggcgg ccgtcagcac cggggccgcg
      901 ccggccggca gcgatcacga ccacgcggtg ctgaccgagc gcctgcgcga cgccgagaac
      961 gaactcggcg cgacgcgcga gcacctgcag gcggtcatcg aggaactcga gacctcgaac
     1021 gaggagctgc agtcgctgaa cgaggaactg caggcctcga ccgaggaact gcagagctcg
     1081 aacgaggagc tcgaaaccac caacgaggaa ctgcaggcca ccaacgagga gctgaccacc
     1141 gtcaacgacg agctggaggc gaaatccgag gagctcacgc aggtcaacac ccagctcgtc
     1201 aacatccagc agtcggtggc gctcggcctg atcgtcgtcg acgccgacct gcgcgtgatg
     1261 cgctacaacc cgccggccgt gcgcgtgttc ggcctgatga acgacgacct cggccagagc
     1321 ctgctggccg taccgtcctt cgtcgagatc ggcgacctgc gccgcaagct gctcgacgtg
     1381 gtcggcagcg gccgcacgct gtccgaggaa ctgcgccgcg aggaagccac cttcctgatg
     1441 cagatcgcgc cgtatcgcga ggacagcggc gtgatctccg gcgccgtgat cacgctgtcg
     1501 gacatcacgc gctttcgcga gctggagcgc gcacacgccg agggcgacca gcgcctgcgc
     1561 ctgatcgccg acacgctgca ggacgccatc tggctggcga ccccgggctt cacgcgcatg
     1621 ctctacgtca gccgcggcta cttcgcgctg tgggaacgca cgcccgaatc gctgtacgcc
     1681 gatccgcatt ccttcctcga tgccgtgcat ttcgaggatc gcgcgcgcgt gcgccgccag
     1741 ctgctggaaa ccgatccgcc ggccaccgac ctgacctacc ggctgctggt gcccggcgcc
     1801 ggccagcgct gggtgcgcca gtgcacgtcg ctggtgcgct gcgaagaaag cggcccggcg
     1861 ctgtatctgg ccaccagcgt gcgcgacgtg accgcggccg ccgccgagcg cgagcggctg
     1921 cagcacagcg aaatgaacct gcgcacggcc atcgacctgc tgccgatcgc cgcgctgctg
     1981 cgcgacgccg agggccgcgt ggtggt
//
LOCUS       DAOQJH010000584         2006 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_45787_length_2006_cov_3.380318, whole genome shotgun sequence.
ACCESSION   DAOQJH010000584 DAOQJH010000000
VERSION     DAOQJH010000584.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2006)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2006)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2006
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45787_length_2006_cov_3.380318"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..887
                     /locus_tag="PLQ18_12030"
     CDS             <1..887
                     /locus_tag="PLQ18_12030"
                     /inference="COORDINATES: protein motif:HMM:NF005741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HNE02424.1"
                     /translation="DAGIGAIVMHSLFEEDLLEDVTQRQLLGLPTDPDSQRALFRGSL
                     DRYLKQLEKLKKSVGVPVIASLNGVSASGWMEQAKALVDAGADALELNVYYLAASPAE
                     SSTAVEARYIELVRQLRATLRIPFAVKLSSQFTALPHFLRRLEAEGASGFVLFNRFYQ
                     PDIDLNGVRLIHRPIRSRSEDVLMALHWIAMLRGRINGGLSASGGVQHADDVLKLLLA
                     GADTVQIVGAVLNEGPGLVSDILAGVGEWLDAHGYDSVADLRGRFSENRISDPGAYER
                     IHYRGVLDNWRAPGEAWR"
     gene            complement(930..1919)
                     /locus_tag="PLQ18_12035"
     CDS             complement(930..1919)
                     /locus_tag="PLQ18_12035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011912112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HNE02425.1"
                     /translation="MQSLDLDVLASAAGWRRSGEAVALATVLATYGSAPRPPGAIAAI
                     RVRDGAITGSVSGGCVEEELVARLARTGPPALPQRIVFGATREEAVRLQLPCGGTLHL
                     LLETLTDPAWIDTVLAATAAGGHRARELNLASGAQRLLPADAMTAFSGDGLLLCQPFG
                     PRWRLLLIGATDVAALLAPMAQALGYAVAVCDPRPGHAGQPLAEGVTRLGGSPDDAVL
                     AFAPDAHSAILTLAHDPRLDDLALMEALASPAFYVGALGSQRTSQARRARLAEVGVEA
                     AAIERLHAPVGLAIGSRTPAEIAVSILAELVAVRARLGQGGAQPQAAAPELAA"
BASE COUNT          285 a          740 c          725 g          256 t
ORIGIN      
        1 ccgatgccgg catcggcgcg atcgtcatgc attcgctgtt cgaggaggac ctgctcgaag
       61 acgtgaccca gcgccagctg ctcggcctgc cgaccgaccc ggacagccag cgcgcgctgt
      121 tccgcggctc gctcgatcgt tacctgaagc agctcgaaaa gctgaagaag agcgtcggcg
      181 tgccggtcat cgccagcctc aacggcgtct cggccagcgg ctggatggag caggcgaaag
      241 cgctggtcga tgccggcgcc gatgcgctgg agctgaacgt ctattacctc gctgcctcgc
      301 cggccgaatc gtcgacggcg gtcgaggcgc gctacatcga gctggtgcgc cagctgcgcg
      361 cgacgctgcg gattccgttc gcggtcaagc tgtcctcgca gttcacggcg ctgccgcact
      421 tcctgcgccg gctcgaagcc gagggcgcca gcggcttcgt gctgttcaac cgcttctacc
      481 agccggacat cgacctcaac ggcgtgcgcc tgatccaccg cccgatccgc tcgcgctccg
      541 aggacgtgct gatggcgctg cactggatcg cgatgctgcg cgggcgcatc aacggcggtc
      601 tgtcggccag cggcggcgtg cagcacgccg atgacgtgct gaaactcctg ctcgccggcg
      661 ccgacaccgt gcagatcgtc ggtgcggtgc tcaacgaagg tccgggcctg gtctccgaca
      721 tcctcgccgg cgtcggcgaa tggctggacg cgcacggcta cgactcggtc gccgacctgc
      781 gcggacgctt cagcgagaac cgcatctccg accccggcgc ctacgagcgc atccactacc
      841 gcggcgtgct cgacaactgg cgcgcgccgg gcgaagcctg gcgctgaacg acgcggcgcg
      901 gccgggcggc accgccgccc ggcatgccgt caggccgcca gctccggcgc agccgcctgc
      961 ggctgcgcgc cgccctgacc gaggcgggcg cgcacggcga ccagctcggc gaggatggac
     1021 acggcgatct cggccggcgt gcggctgccg atcgccagcc cgaccggcgc gtgcagccgc
     1081 tcgatcgccg cggcttcgac gccgacctcg gcgagccgcg cgcggcgcgc ctgcgaagtc
     1141 cgctgcgagc cgagcgcacc gacgtagaag gccggcgagg cgagcgcttc catcagcgcc
     1201 agatcatcga gccgcgggtc gtgcgcgagc gtgaggatcg cgctgtgcgc atccggcgcg
     1261 aaggcgagca cggcgtcatc cggcgaaccg ccgaggcgcg tgacaccttc ggcgagcggc
     1321 tgtccggcat gaccggggcg cggatcgcag accgcgaccg cgtagccgag cgcctgggcc
     1381 atcggtgcga gcagcgcggc gacatcggtc gcgccgatca gcagcagccg ccagcgcggg
     1441 ccgaagggct ggcacagcag caggccgtcg ccgctgaagg ctgtcatcgc gtcggccggc
     1501 agcagccgct gcgcgccgct ggcgaggttc agctcgcggg cgcgatgccc gccggcggcg
     1561 gtggccgcga gcaccgtgtc gatccaggcc ggatcggtca gcgtctccag cagcaggtgc
     1621 agcgtgccgc cgcagggcaa ctgcaggcgc acggcctcct cgcgcgtcgc gccgaacacg
     1681 atgcgctgcg gcagcgccgg tggtccggtg cgcgccagac gcgcgaccag ttcctcctcg
     1741 acgcagccgc cggacaccga accggtgatc gcaccgtcgc gcacgcggat cgccgcgatc
     1801 gccccgggcg ggcgcggagc cgagccgtag gtggcaagca cggtcgcgag cgcgaccgcc
     1861 tcgccgctgc gccgccagcc ggcggcgctt gcgagcacat ccagatcgag cgactgcatg
     1921 ctcacgcgct ccgtcagctc ttggcgaccg gcatcagcgg cagccggcgg tggcgttcgc
     1981 cggtcagcgc gaacagcgcg ttgccg
//
LOCUS       DAOQJH010000585         2004 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_45875_length_2004_cov_4.111339, whole genome shotgun sequence.
ACCESSION   DAOQJH010000585 DAOQJH010000000
VERSION     DAOQJH010000585.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45875_length_2004_cov_4.111339"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            188..1768
                     /locus_tag="PLQ18_12040"
     CDS             188..1768
                     /locus_tag="PLQ18_12040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome D1 domain-containing protein"
                     /protein_id="HNE02426.1"
                     /translation="MRPSLSLLPALLLASLSAAPAAPAAAQPSDAASAGPAPALYAQH
                     CASCHGATRLGGMGPALLPENLGRLKPAEALATIRDGRAATQMPAFAAKLDTAQLQAL
                     ADFVYTPPAVAPVWGIDEIRASRTFDAEAARLPARPVFDADPRNLFVVVELGDHHVTV
                     LDGDRFRPLTRFASRFALHGGPKFSPEGRFVYFCSRDGWVTKYDLWNLRTVAEVRAGI
                     NARNLAVSDDGRFVMVANYLPQTLVLLSADELAPLKLYPVADAAGHGSRVSAVYTAAP
                     RGSFVAALKDVQEVWEIPYRDDLPVHAGPMQDDRAGAAGRSAALEPRRIALDDVLDDF
                     FFDPAYRHLIGASRDGGKGQVIDLDAGTRVATLDLPGMPHLGSGITWEWQGRPVLATP
                     NLKESAITVIDMTNWQVIKRIPTLGPSFFMRSHAQLDTAWIDVSLGRERDAMQLIDKN
                     TLEIRTTIRPQPGRTAAHVEFTQDGRQALISIRERDGALVVLDTQSLTEITRLPMAMP
                     SGKYNVHNKITRDAGTSH"
BASE COUNT          313 a          765 c          644 g          282 t
ORIGIN      
        1 ccgtccgcgc ccgtcatacc gatcagacgg aaatgcacgg tttttccccc gctccccaac
       61 ccctctccca cgaggggcga ggggctgaaa aacgaagcat tccggtgtga tcggcataac
      121 tgccggacgg cagcccccca gtccttgcga gcgaccgcca ccggtcgccg aaccggaaac
      181 cgccgtcatg cgcccgagcc tgtccctgct gcccgccctg ctgctcgcca gcctgagcgc
      241 cgcccctgcg gccccggccg cggcgcagcc atcagatgcc gcctccgccg gcccggcacc
      301 ggcgctgtac gcgcagcact gcgcgagctg ccacggggcg acgcgcctcg gcggcatggg
      361 tccggcgctg ctgccggaga acctcgggcg gctgaagccg gccgaggcgc tggcgacgat
      421 ccgcgacggc cgcgccgcca cgcagatgcc ggcctttgcg gcgaagctcg acaccgcgca
      481 gctgcaggcc ctcgccgatt tcgtctacac gccgccggcg gtcgcgccgg tctggggaat
      541 cgacgagatc cgcgcaagcc gcaccttcga tgccgaagcc gcgcgcctgc cggccagacc
      601 ggtgttcgac gccgatccgc gcaatctctt cgtcgtcgtc gaactcggcg atcaccacgt
      661 caccgtgctc gacggcgacc gcttccggcc gctcacgcgc ttcgcatcgc gcttcgcgct
      721 gcacggcggc ccgaagttct cgcccgaggg ccgcttcgtg tacttctgct cgcgcgacgg
      781 ctgggtcacg aagtacgacc tctggaacct gcgcaccgtc gccgaggtgc gtgccggcat
      841 caacgcccgc aacctcgccg tcagcgatga tggccgcttc gtgatggtcg ccaactacct
      901 gccgcagacg ctggtgctgt tgtcggccga cgagctcgcg ccgctgaagc tctacccggt
      961 cgccgatgcc gccggccacg gctcgcgggt cagcgccgta tacacggccg caccgcgcgg
     1021 cagcttcgtt gcggcgctga aggacgtgca ggaggtctgg gaaatcccgt accgcgacga
     1081 tctgccggtc cacgccggcc cgatgcagga cgatcgcgca ggcgccgccg gccgcagcgc
     1141 cgcgctggag ccacgccgca tcgcgctcga tgacgtgctc gatgacttct tcttcgatcc
     1201 cgcctaccgc cacctgatcg gcgcctcgcg cgacggcggc aagggtcagg tcatcgacct
     1261 cgatgccggc accagggtcg caacgctcga tctgcccggc atgccgcatc tcggttccgg
     1321 catcacctgg gaatggcagg gccggccggt gctggcgacg ccgaacctga aggaatcggc
     1381 gatcaccgtc atcgacatga ccaactggca ggtcatcaag cgcattccga ccctcggccc
     1441 gagcttcttc atgcgcagcc acgcgcagct cgacaccgcc tggatcgatg tctcgctcgg
     1501 acgcgagcgc gacgcgatgc agctgatcga caagaacacg ctggagatcc gcacgaccat
     1561 ccgcccgcag ccgggccgca ccgccgcaca cgtcgagttc acgcaggacg gccgccaggc
     1621 gctcatcagc atccgcgaac gcgacggcgc gctggtcgtg ctcgacacgc agagcctcac
     1681 cgagatcacc cggctgccga tggcgatgcc ctcgggcaag tacaacgtgc ataacaagat
     1741 cacgcgcgac gccgggacca gtcactgaca aatcagtgac ctgcgtcaga tttcagcgca
     1801 tccgcagccg cagtggcccc gccgtgtccg ggccgcccgg caagcacgcg cagcgccagc
     1861 gcagcccccg gatgggggcg acggcttcag cgtgcgtggc gccgcaagcc atgacgcccg
     1921 gccgttccgg gtgcaagcgg tgcgtcgccg gcgccgtcta ctgcggcagg aagtcgtcgg
     1981 ccatcacttc gccggggctg tacg
//
LOCUS       DAOQJH010000586         2001 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_46011_length_2001_cov_3.412641, whole genome shotgun sequence.
ACCESSION   DAOQJH010000586 DAOQJH010000000
VERSION     DAOQJH010000586.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46011_length_2001_cov_3.412641"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(29..1435)
                     /locus_tag="PLQ18_12045"
     CDS             complement(29..1435)
                     /locus_tag="PLQ18_12045"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HNE02427.1"
                     /translation="MQRLSAQDASFITLESSTRPMHISFLLTFKTPPGAPADYMQKLH
                     EKLRRFPVDVEPFNWRLVPQSGLARLVPAWETVADVDLDYHLRHEALPWPGGEAELGM
                     VVSRLHTTPLDRSRPLWEFYLIEGLERDRFAVYMKLHHALTDGLVMMQQVTAILSTSP
                     RGRNLPIWATPLATADTKADTKPESSQDKLARFLEGLMTPPGKRQPDSPQAESGGPLL
                     YGPKSILNGPITGRRRLATQSLDLNRIKAVAKATETTVNDVVLAVCAGALRRYLAESR
                     DLPNKPLIATIPVALPRSQGQVAGNSVGGMNASLATDVKDAGKRLRAIHASVRAAKSQ
                     FRRIPPALNKVFSSIGMLAMMLMPDKNDADSEQAKFTNLTISNVPGPKATLYLNGAEL
                     DGMYPVSVLSGDQRLNITVLGYRDRLFFGLTGCPDGLPRMQRLAVYLPEALGELELSV
                     GGRGPAAARRRGGRAEGA"
     gene            1608..>2001
                     /locus_tag="PLQ18_12050"
     CDS             1608..>2001
                     /locus_tag="PLQ18_12050"
                     /inference="COORDINATES: protein motif:HMM:TIGR02610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoic acid system family protein"
                     /protein_id="HNE02428.1"
                     /translation="MSKIDIRRAHALPLRQAKKQADIMAEHLGREFGLRSRWVQGRLH
                     FERSGVEGVMTVTETEVQVQAELGFAVSLLRTTIERRLHSHFDTAFAADEAAGGKGSA
                     ARSTKAARSPAAAPETTAARKTTAARKTT"
BASE COUNT          299 a          705 c          689 g          308 t
ORIGIN      
        1 gtccggtgcg cgggcgcgat tcatgtcttc acgcgccctc ggcgcgcccg ccgcgccgcc
       61 gcgccgccgc cgggccgcgc ccgcccacgc tgagctcgag ctcgcccagc gcctcgggca
      121 ggtagaccgc gagccgctgc atcctcggca ggccgtccgg gcagccggtc agaccgaaga
      181 acagccggtc gcgatagccg agcacggtga tgttcaggcg ctggtccccc gacaacaccg
      241 ataccggata catgccgtcg agctccgcgc cgttcaggta cagcgtcgcc ttcggtccgg
      301 gcacgttgga gatcgtcagg ttggtgaact tcgcctgctc gctgtcggca tcgttcttgt
      361 cgggcatcag catcatcgcc agcatcccga tcgagctgaa caccttgttg agcgccggcg
      421 gaatgcggcg gaactggctc ttggccgcgc gcaccgaggc gtggatcgcg cgcaggcgct
      481 tgccggcgtc cttcacgtcg gtggcgagcg aggcgttcat gccgcccacc gaattgcccg
      541 ccacctgccc ctgcgagcgc ggcagcgcga ccggaatcgt ggcgatcagc ggcttgttcg
      601 gcagatcccg ggactcggcc agatagcggc gcagggcacc ggcacatacg gccagcacga
      661 cgtcgttgac cgtcgtttcg gtggctttcg cgacggcctt gatccggttc aggtcgagcg
      721 actgcgtcgc caggcggcgc ctgcccgtga tcggcccgtt caggatcgac ttcgggccgt
      781 acagcagcgg gccgccgctc tcggcctggg ggctgtcggg ctgccgtttg ccgggcggcg
      841 tcatcagtcc ctcgagaaag cgcgcgagct tgtcctggct cgattcgggc ttcgtgtcgg
      901 ccttcgtgtc ggccgtcgcg agcggggtcg cccagatcgg cagattgcgt ccgcgcgggg
      961 aagtcgacag gatcgcggtg acctgctgca tcatcaccag gccgtcggtg agcgcatggt
     1021 gcagcttcat gtagaccgcg aagcggtcgc gctcgaggcc ttcgatcagg tagaactccc
     1081 acagcggccg cgagcggtcg agcggcgtcg tatgcaggcg cgacaccacc atgccgagct
     1141 cggcttcgcc gcccggccag ggcagtgcct cgtggcgcag gtggtagtcc aggtcgacgt
     1201 cggcgaccgt ctcccaggcc ggcaccagcc gcgccagccc gctttgcggc accagccgcc
     1261 agttgaacgg ctcgacatcg accggaaacc gacgcagctt ctcgtgcagc ttctgcatgt
     1321 agtcggccgg cgccccgggc ggcgtcttga acgtcagcag gaagctgatg tgcatcggcc
     1381 tcgtcgacga ttcgagcgtg atgaacgacg cgtcctgcgc actcagcctt tgcatgtctt
     1441 gtctcccgat gttctcgctc gctgccgccc gggccgaggc gcagaacgcg cgcgcgaccc
     1501 cacgccccca ccatagtatg gccggctgcg gcgcgtctta ccacatccgg actagcggca
     1561 cgaagtccgc tacgatgcgc gccgagcaca gctgcccagg caccccgatg agcaagatcg
     1621 acattcgccg cgcgcacgca ctgccgctgc gccaggcgaa gaagcaggcc gacatcatgg
     1681 ccgaacatct ggggcgcgag ttcggcctgc gctcccggtg ggtgcagggc aggctccact
     1741 tcgagcgctc cggggtcgaa ggcgtgatga ccgtgaccga gaccgaggtg caggtgcagg
     1801 ccgagctcgg cttcgcggtc tcgctgctca ggacgacgat cgagcggcgc ttgcattcgc
     1861 atttcgacac ggcattcgca gcggacgagg cagcgggcgg caagggcagt gcggcgcgca
     1921 gcacgaaggc cgcgcgcagc cccgccgccg cgcccgagac gaccgccgcg cgcaagacga
     1981 ccgccgcgcg caagacgacc g
//
LOCUS       DAOQJH010000587         1998 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_46146_length_1998_cov_2.646423, whole genome shotgun sequence.
ACCESSION   DAOQJH010000587 DAOQJH010000000
VERSION     DAOQJH010000587.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1998
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46146_length_1998_cov_2.646423"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..687
                     /locus_tag="PLQ18_12055"
     CDS             <1..687
                     /locus_tag="PLQ18_12055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007514118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNE02429.1"
                     /translation="VRKDRDGKAEDRLENLDELINAARAFETLPPEYEGLTPVDAFLA
                     HASLEAGDAQAEAWSDCVQLMSLHSAKGLEFPQVFIAGVEEELFPHALSMQDPEKLEE
                     ERRLAYVGITRARERLVLSCAERRRLHGQEHYPRPSRFLSEIPPGLLREVRARAQISR
                     PVYQPPVAAAPDDGGSGLKPGARVLHPSFGEGVVLTLEGSGAHLRVQVNFPGQGARWL
                     VAAYAKLEVR"
     gene            782..1066
                     /locus_tag="PLQ18_12060"
     CDS             782..1066
                     /locus_tag="PLQ18_12060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02430.1"
                     /translation="MRNRLVHIAPLQTAKVLAAIYFVISVPIALLMAIPAVLGFGQHG
                     AGMALLMVILTPFLYALAGFLFTLLGAWIYNLVAARLGGIEYTTITAVER"
     gene            complement(1155..1631)
                     /locus_tag="PLQ18_12065"
     CDS             complement(1155..1631)
                     /locus_tag="PLQ18_12065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02431.1"
                     /translation="MKWADLHQGQWIHGKRIGSVDKLGGMHGLPEQTVLAQLLNKAQN
                     LNLKPVYTGMSFTWCRAVVTNHQVSCQNESSQTDTDSKSPEICHEDRVFPVSPGDGHL
                     LIRQNLAPCARCRAAFRAWAKSRGSTIIVVSDKGYDSSPDNTIFIFGPTGAAYQWY"
BASE COUNT          293 a          676 c          696 g          333 t
ORIGIN      
        1 gtgcgcaagg atcgcgacgg caaggctgag gaccgcctcg aaaacctcga cgagctgatc
       61 aacgccgcgc gcgccttcga gaccctgccg cccgagtacg agggcctgac gccggtcgat
      121 gccttcctcg cgcatgcctc gctggaggcc ggcgacgctc aggccgaggc ctggagcgac
      181 tgcgtgcagc tgatgagcct gcacagcgcc aaggggctgg agttcccgca ggtgttcatc
      241 gccggcgtcg aggaggagct tttcccgcac gcgctgtcga tgcaggaccc ggaaaaactc
      301 gaagaggaac ggcgcctcgc ctacgtcggc atcacccgtg cgcgcgaacg cctggtgctg
      361 tcctgcgccg agcgccgccg gctgcacggt caggagcact acccgcggcc gtcgcgcttt
      421 ttatcggaaa tcccgcccgg gctgctgcgc gaggtgcgcg cccgcgcgca gatcagccgc
      481 ccggtctacc agccgccggt cgccgccgcg cccgatgacg gcggcagcgg tctcaagccc
      541 ggcgcgcgcg tgcttcatcc gagctttggc gagggcgtcg tgctgacgct cgaaggcagc
      601 ggtgcgcacc tgcgcgtgca ggtgaacttc cccggccagg gcgccaggtg gctggtcgcg
      661 gcctacgcga agctcgaagt gcggtaggcc ccggcccggc tgcctatgct gcggacagcc
      721 ccgcccgtcg aggcgggcgg tttcttctgt ccgaaatgct gtccgaaccg aggcccttcc
      781 gatgcgcaac cgtctggtcc acatcgcccc gctgcagacc gccaaggtgc tggcagcgat
      841 ctatttcgtc atctcggtgc cgatcgcgct gctgatggcg atcccggccg tgctcggctt
      901 cggtcagcac ggcgccggca tggcgctgct gatggtgatc ctgacgccgt tcctgtacgc
      961 gctcgccggc ttcctgttca cgctgctcgg cgcctggatc tacaacctcg tcgccgcccg
     1021 gctcggcggc atcgagtaca cgacgatcac tgcggtcgaa cgctgaggaa ccggggccgg
     1081 agccggcgct ccggtcctgc gccttgcagg cattggcggc tgcgcagctt cagcgagttt
     1141 cgccggccgc ggcatcagta ccactgatac gcggcgccgg tcgggccgaa gatgaagatg
     1201 gtgttgtccg gcgagctgtc gtagcccttg tccgaaacca cgatgatcgt cgagccccgg
     1261 ctcttggccc atgcccggaa ggcggcgcga cagcgcgcgc agggcgcgag gttctggcgg
     1321 atcagcagat gcccgtcgcc gggcgaaacc ggaaacacgc ggtcctcgtg gcagatttcc
     1381 ggggatttgc tgtcggtgtc ggtctgcgag ctttcgttct ggcaggacac ctgatgattg
     1441 gtgacgacgg cccggcacca cgtgaaggac atgccggtat agaccggctt cagattcagg
     1501 ttctgcgcct tgttcagcag ctgcgccagc accgtctgtt cgggcaggcc gtgcatgccg
     1561 ccgagcttgt cgacgctgcc gatgcgtttg ccgtgaatcc attggccctg atgcaggtcg
     1621 gcccatttca tgatctgtct ccggttacgg gtcgggggat gcgcttcgtg caggccgtgc
     1681 cgacgcgcgc cgcggcgcgg gtcgatgcgg tccatgcctg ccgatacggg gccggaggcg
     1741 atccggagac aggggcggcg cagatttccc gagcgccggc cgcagttgca gaccaatgca
     1801 ggcggcggat gcgccggcag cggccgcagc ctgacaccac cggcccgctg tgtttcgcgt
     1861 ccggcaacag gaggcgcaat gctgcggcgc cgactacgct cgcagcctca gggatccatg
     1921 actgccgcga ggggcaaggg aggggcaggg ctgtgcaacg ctcccctagt agtgcaaagt
     1981 gtcgcgccct cacagccg
//
LOCUS       DAOQJH010000588         1996 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_46210_length_1996_cov_3.635755, whole genome shotgun sequence.
ACCESSION   DAOQJH010000588 DAOQJH010000000
VERSION     DAOQJH010000588.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1996)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1996)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1996
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46210_length_1996_cov_3.635755"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(102..1394)
                     /locus_tag="PLQ18_12070"
     CDS             complement(102..1394)
                     /locus_tag="PLQ18_12070"
                     /inference="COORDINATES: protein motif:HMM:NF025611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4347 domain-containing protein"
                     /protein_id="HNE02432.1"
                     /translation="MTRNPRRPAGSRSPASLMLALEPRLMFDAAALSSAADTLTHADP
                     AGEAAVAAVEAARASDSPAAAGTASDSAAAAGTATVATAAPAPTADTGTTAPALLAAI
                     ASDSPQTTAADATVASAPAGQRHELAIIDTSLTGWQTLAAGLRDGIEVLTIDGSRDGF
                     AQLADALAGRERGGLDAIHVFSHGSEGQWRLGSSALDVNTEAAHASELQAIAAALAAD
                     GDLLLYGCDTGAGSRGTAFLDRLASATGADVAASTDRSGSATAGGNWTLEAQRGAIET
                     ATAADAAKLADYNGTLATYTVTSTADSGAGSFRQAVLDANASGGTDTIVFDNSLDNQT
                     ITLTGGVISVTDSLIIDGDLDGDDTPDVILSGGGASRGLDYTSSSTTGIALTVRSLEF
                     ANFSSTTANAGGAIHFFLNTTPNAIKLAKNLSLISRLH"
     gene            complement(1515..>1996)
                     /locus_tag="PLQ18_12075"
     CDS             complement(1515..>1996)
                     /locus_tag="PLQ18_12075"
                     /inference="COORDINATES: protein motif:HMM:NF024829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HNE02433.1"
                     /translation="VAALADGLLPGVWLSPRQTLAQIIDPAAARIEALVPEDRLTTVA
                     RAVADGAAARFHPDTPERPVLGALVLALAPAASRTLDAQPLLASVYGGPLAATRDAHG
                     RLLPQTPVYRLDLQPAAGAAAPERRLRGTLVLEVEAEPLLTQVWRRIAAVLIRESGF"
BASE COUNT          257 a          648 c          781 g          310 t
ORIGIN      
        1 caaaggcttc agagcggata tgggcgccga gcgcttcgaa gatggcgtgc aaattgagcc
       61 gttactatgg tggaactgtc ggttcgacag gcgaggcgat tttagtgcaa ccggctgatc
      121 aggctgagat ttttggctaa cttaatggca ttgggagtag tgttgaggaa gaaatggatg
      181 gcaccgccgg cattcgcggt ggtgctgctg aaattcgcaa attccaggct gcgcacggtc
      241 agtgcgatgc cggttgtcga tgaactggtg taatccaggc cgcgactggc gccacctccg
      301 ctcaggatca catccggcgt gtcgtcgccg tcgaggtcgc catcgatgat caggctgtcg
      361 gtcacgctga tgacgccgcc cgtgagcgtg atcgtctggt tgtcgaggct gttgtcgaac
      421 acgatggtgt cggtgccgcc gctggcgttg gcatcgagca cggcctgacg gaagctgccg
      481 gcgccgctgt cggcggtgct cgtgaccgtg taggtcgcca gcgtgccgtt gtaatccgca
      541 agcttcgcgg catcggccgc cgtcgcggtt tcgatcgcgc cacgctgcgc ttccagcgtc
      601 cagttgccgc cggccgtggc gctgccgctg cgatcggtcg aggcggccac gtcggcgccg
      661 gtggcgctgg caaggcgatc gaggaaggcc gtgccgcggc tgccggcgcc ggtgtcgcag
      721 ccgtagagca gcaggtcgcc atccgcggcg agcgcggcag cgatggcctg cagttcgctc
      781 gcgtgtgcgg cctcggtgtt gacatcgagc gcgctgctgc cgagccgcca ctggccctcg
      841 ctgccgtggc tgaagacgtg gatggcatcg agcccgccac gctcgcgccc ggcgagcgca
      901 tcggcgagct gcgcgaagcc gtcgcggctg ccgtcgatcg tcagcacctc gatgccgtca
      961 cgcaggccgg cggcgagtgt ctgccagccg gtgagcgagg tgtcgatgat ggccagctca
     1021 tggcgctgcc cggccggggc gctggccacg gtggcgtccg cagccgtcgt ctgcggcgaa
     1081 tcgctcgcga tcgctgccag cagtgccggt gcggtcgtac cggtgtcggc cgtcggggcc
     1141 ggcgcggcgg tggcgacggt cgcagtaccg gcggccgcgg cactgtcgct ggccgtgccg
     1201 gccgcagccg ggctgtcgct ggctctggcg gcctcgaccg cggccaccgc cgcctcgccg
     1261 gccgggtcgg cgtgggtgag cgtgtccgcg gcgctgctga gggcggcggc gtcgaacatc
     1321 aggcgcggct ccagcgccag catcaggctc gcggggctgc ggctgccggc agggcgacgg
     1381 gggttgcggg tcatgtcggc tctccagtcc tgcgagttcg tgccggcgcc atcgggcgtg
     1441 ccggagttcg gggcaaggtt cgggtcaggg tgcggttcag gcgcatccgc caccggcgcc
     1501 gggcgccggc cggttcagaa gccgctctca cggatcagca ccgcggcgat ccggcgccag
     1561 acctgcgtca gcagcggctc ggcctcgact tcgagcacca gcgtgccgcg cagccggcgc
     1621 tcgggcgcag cggccccggc ggcgggctgc aggtcgagcc ggtagaccgg cgtctgcggc
     1681 agcagccggc cgtgggcatc gcgggtcgcg gcgagcggcc cgccgtacac ggaggcaagc
     1741 agcggctgcg catcgagcgt gcggctggcg gccggggcga gggcgagcac gagcgcgccg
     1801 agcaccgggc gttcgggggt gtcgggatga aagcgtgccg cggcaccgtc ggcgacggca
     1861 cgggcgacgg tcgtgagccg gtcttcaggc accagcgcct cgatgcgcgc cgcagccggg
     1921 tcgatgatct gtgcgagcgt ctggcgcggc gacagccaga cgccgggcag caggccatcg
     1981 gcgagcgcgg caacac
//
LOCUS       DAOQJH010000589         1990 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_46492_length_1990_cov_4.274935, whole genome shotgun sequence.
ACCESSION   DAOQJH010000589 DAOQJH010000000
VERSION     DAOQJH010000589.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1990
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46492_length_1990_cov_4.274935"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..170)
                     /locus_tag="PLQ18_12080"
     CDS             complement(<1..170)
                     /locus_tag="PLQ18_12080"
                     /inference="COORDINATES: protein motif:HMM:TIGR04366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbuC family cupin fold metalloprotein"
                     /protein_id="HNE02434.1"
                     /translation="MPVLFDSALLDLLRNHARDSPRRRAHHPLHRSLDEPCQRLLVAI
                     EPDSYVPPHRHL"
     gene            complement(287..652)
                     /locus_tag="PLQ18_12085"
     CDS             complement(287..652)
                     /locus_tag="PLQ18_12085"
                     /inference="COORDINATES: protein motif:HMM:NF024879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper domain-containing protein"
                     /protein_id="HNE02435.1"
                     /translation="MSALASLPRTSVTRHPLLVLLLAAPLAIGAVGCGHGPATDAGVG
                     ALTGAAVGAGVGGRKGALVGGVVGAAAGVAIGQEQERRRAEAERARYYDDGYGRNYDR
                     SYNRGYQDGYRRDPYYDNY"
     gene            725..1027
                     /locus_tag="PLQ18_12090"
     CDS             725..1027
                     /locus_tag="PLQ18_12090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02436.1"
                     /translation="MKIKNMMRYSGYGPCSGPALLSGCGDNVRFWAVGALDGLDAGRT
                     ARGAGRRPFCMAVRPFALIVPRCEPPAGSRRLIPTQAALRALSSERSAQAGRGAGA"
     gene            1024..1551
                     /locus_tag="PLQ18_12095"
     CDS             1024..1551
                     /locus_tag="PLQ18_12095"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HNE02437.1"
                     /translation="MNGFHPAAGVSVRAVEAADHAAIATVVDTAFAAAAHADGTEAAL
                     VAELRAAGGIACEWVAVEGAGPVGHIAFSAVTLDGRDCNWFGLAPLAVAPAYQGRGIG
                     ARLVRTGLAALQARGAGGCVVLGEPAYYARFGFAALPVLVCAWPVPPQYFQALAWQPL
                     ACAGIVRYHPLFGAV"
     gene            1740..1988
                     /locus_tag="PLQ18_12100"
     CDS             1740..1988
                     /locus_tag="PLQ18_12100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012971006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HNE02438.1"
                     /translation="MLNFEETRALIGDVLQLGERTAEIHEDTLLLGSIPEFDSMAVVS
                     LITAIEDRFGIAIDDDELIADTFASAGSLHAFIAARVD"
BASE COUNT          278 a          669 c          730 g          313 t
ORIGIN      
        1 tgcagatggc ggtgcggcgg gacgtaggaa tccggctcga tcgcaaccag caggcgctgg
       61 cagggttcat cgaggctgcg gtgcagcggg tgatgcgcac ggcggcgcgg gctgtcccgt
      121 gcatggttgc gcagcaggtc cagcagggcg gaatcgaaca gcacgggcat cgcggacggt
      181 ccggcgtcgg aaagggcagg tccggcagcc taccggctgc gggccgtcca ctcacgcgga
      241 tgtgccgggc gcagccgccg gaaaccggag cccggtcacc tcaggctcag tagttgtcgt
      301 agtacggatc gcgacggtag ccgtcctgat agccgcggtt gtagctgcgg tcatagttgc
      361 gtccgtagcc gtcgtcgtaa tagcgcgcac gttcggcctc ggcccggcgg cgttcctgtt
      421 cctgaccgat cgcgacaccg gcagcggctc cgaccacgcc accgacgagg gcaccctttc
      481 tgccgccgac gccggcaccg actgctgcac cggtcagcgc accgacgccg gcatcggtgg
      541 ccgggccgtg gccgcagccg actgccccga tggcgagcgg ggcggcgagc agcagcacca
      601 gcagcggatg acgggtgacg gacgtgcggg gcagcgacgc gagggcggac atgatggcgg
      661 ctcctgacag gccgggaacg ggtgagggag acagagcact tgtagtgcca tgccggtgtt
      721 attcatgaaa atcaaaaaca tgatgcgtta ttccggatac ggaccctgct cgggaccggc
      781 gctgctgtcg gggtgcggcg acaacgtccg gttttgggcg gttggtgcgc tcgatgggct
      841 ggatgccggc aggacggccc ggggagcggg ccgcaggccg ttctgcatgg ccgtccgccc
      901 gtttgcgctc atcgtgccgc gctgcgagcc gcctgccggc agccgtcgtc tgatcccgac
      961 gcaggcagcc ttgcgtgcgc tctcgagcga gcgcagcgcg caggccggcc gcggggccgg
     1021 cgcatgaacg gcttccatcc ggctgcaggg gtttcggtgc gcgccgtcga ggctgccgat
     1081 cacgccgcga tcgccaccgt cgtcgatacc gccttcgccg ctgcggcgca tgccgatggc
     1141 accgaggcgg cgctggtggc cgagctgcgg gccgccgggg gcatcgcctg cgagtgggtg
     1201 gccgtcgagg gcgcggggcc ggtcggccac atcgccttca gtgccgtgac gctcgatggt
     1261 cgcgactgca actggttcgg gctggcgccg ctggcggtgg caccggcgta tcagggccgc
     1321 ggcatcgggg cgcggctggt gcgcacgggg cttgcggcgc tgcaggcgcg cggcgccggc
     1381 ggctgcgtcg tgctcggcga accggcctat tacgctcgct tcggcttcgc cgcgctgccg
     1441 gtgctggtct gcgcctggcc ggtgccgccg caatatttcc aggcactcgc ctggcagccg
     1501 ctggcatgtg ccggcatcgt gcggtatcac ccgctgttcg gagcggtctg agccttcggc
     1561 atcgtgatgg cgcaatcttg cggaaacttc cgggaatcct ggccgatgcc gctgttcaca
     1621 agcgtgcgac tggcgcgcgc agggccgctc cggtatcttt ccgcgccctg tgcgtcaccg
     1681 gcccgatccg ggtcgtcgcg ccacaggcag ccccgatccc gagcccggaa gcggtcccca
     1741 tgctgaactt cgaagaaacc cgcgccctca tcggcgacgt gctgcagctc ggcgagcgta
     1801 ccgccgaaat ccacgaagac acgctgctgc tcggcagcat ccccgaattc gactcgatgg
     1861 ccgtggtcag cctgatcacc gcgatcgagg accgcttcgg catcgcgatc gacgacgacg
     1921 agctgatcgc cgataccttc gcgagtgccg gcagcctgca cgccttcatc gcagcccgcg
     1981 tcgactgaag
//
LOCUS       DAOQJH010000590         1987 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_46624_length_1987_cov_5.182712, whole genome shotgun sequence.
ACCESSION   DAOQJH010000590 DAOQJH010000000
VERSION     DAOQJH010000590.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1987)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1987)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1987
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46624_length_1987_cov_5.182712"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..199)
                     /locus_tag="PLQ18_12105"
     CDS             complement(<1..199)
                     /locus_tag="PLQ18_12105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005224373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease family
                     protein"
                     /protein_id="HNE02439.1"
                     /translation="MTPSLTLALLLGLTALAYHLGRRRAFTVAGGRVNRLHSLPGYYG
                     LHAALWCALPALALFLIWISLE"
     gene            complement(336..1385)
                     /locus_tag="PLQ18_12110"
     CDS             complement(336..1385)
                     /locus_tag="PLQ18_12110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015281386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HNE02440.1"
                     /translation="MNKPLLALAVTASLAAAAVVQAQSARDYISVVGSSTVYPFTTVV
                     AEQFGKSTKFKTPKIESTGTGGGFKLFCAGVGPEHPDVSNASRAIKTSEIESCAKNGV
                     TDIVEVKIGYDGIVFANAKGAPWVKLTRAQIYLALAKTVPDPKDPTKLVANPYQNWSQ
                     IDAGLPNAKIEVLGPPPTSGTRDAFVELVMDHGCETFPAIKALKDGDPKQFKAVCQTM
                     REDGSFIEAGENDNLIVQKLVANKSAFGIFGYSFMEENLDKVQGSIVEGHSPTFEEIA
                     AGKYPIARPLFFYVKKAHVGVIPGLKEFVNEFVSEKSFGTDGYLADKGLIPLPDAERK
                     SVTAAARELKTIGSK"
     gene            complement(1609..>1987)
                     /gene="glnD"
                     /locus_tag="PLQ18_12115"
     CDS             complement(1609..>1987)
                     /gene="glnD"
                     /locus_tag="PLQ18_12115"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Catalyzes the uridylylation or deuridylylation of
                     the PII nitrogen regulatory protein; Derived by automated
                     computational analysis using gene prediction method:
                     GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HNE02441.1"
                     /translation="MREIMQRLRWQLQHPEAPLAPPSRRAPRALRHFRTPTTVYFAED
                     AAQRHTVLTLITTDRPGLLCRIGHAFMHCGIRVHNARIATIGARAEDIFLITDDAQRP
                     LADPLSQTRLREALERQLDGDPI"
BASE COUNT          301 a          605 c          735 g          346 t
ORIGIN      
        1 cttcgagcga gatccagatc aggaacaggg ccagtgccgg cagcgcgcac cagagggcag
       61 catgcaggcc gtagtagccg ggcagcgaat gcagccggtt cacgcggccg ccggcgacgg
      121 tgaacgcgcg ccgccggccg aggtgatagg cgagggccgt gaggccgagc aggagcgcca
      181 gagtcaggga tggtgtcatc gtcggaaatc cgcggcgggc tggggaatgc cgtgtacggg
      241 ccgcactgcc gggcggggac ggcgcaaggc ggccgggcgc gtcatccgca ccccgccgcc
      301 tgcggcggac ttcacggcgc tcgccggccg gcagcttact tgctgccgat cgtcttcagt
      361 tcgcgcgccg cggcagtgac cgacttgcgc tcggcgtcgg gcagcgggat caggcccttg
      421 tcggcgagat agccgtcggt gccgaaggac ttctcgctga cgaactcgtt gacgaactcc
      481 ttcaggccgg ggatcacgcc gacgtgtgcc ttcttcacgt agaagaacag cgggcgcgcg
      541 atcggatact tgccggcggc gatttcctcg aaggtcggcg agtggccctc gacgatcgag
      601 ccctgcacct tgtcgaggtt ctcctccatg aagctgtagc cgaagatgcc gaaggcgctc
      661 ttgttggcga cgagcttctg cacgatcagg ttgtcgttct cgccggcctc gatgaagctg
      721 ccgtcctcgc gcatcgtctg gcagacggcc ttgaactgct tcggatcgcc gtccttcagc
      781 gccttgatcg ccgggaaggt ttcgcagccg tggtccatga ccagctcgac gaacgcatcg
      841 cgcgtgcccg aggtcggcgg cgggccgagc acctcgatct tggcgttcgg cagaccggcg
      901 tcgatctggc tccagttctg gtacggattg gcgacgagct tcgtcgggtc cttcgggtcc
      961 ggcacggtct tggcgagcgc gaggtagatc tgcgcacgcg tcagcttgac ccacggcgcg
     1021 cccttggcgt tggcgaacac gatgccgtcg tagccgatct tgacctcgac gatgtcggtg
     1081 acgccgttct tcgcgcagct ctcgatctcc gaggtcttga tcgcgcgcga ggcattggaa
     1141 acgtccggat gctcggggcc gacgccggcg cagaacagct tgaagccgcc gccggtgccg
     1201 gtcgattcga ttttcggcgt cttgaacttc gtgctcttgc cgaactgttc ggccacgacg
     1261 gtcgtgaacg gatacacggt cgaggaaccg acgacgctga tgtagtcgcg ggccgactgg
     1321 gcctgcacga cggcagcggc agcgaggctt gccgtcacgg cgagcgcgag aagcggcttg
     1381 ttcacgaaaa cctccggtga gacatgaatt tgtcagatcc gcgcgctggc ggagcaggag
     1441 cttgatctga gggcgcggcg cattctggcc gggcattgct acggtttcat gacagcctgc
     1501 agggttttgt tgcgctgtga tgacagcggg ctgccgtgcc ggcgcagccg gtcgggaccg
     1561 gcggaaacag tgtgcggacc gtggattgca gcgtgatgtc gatgcgcgtc agatcggatc
     1621 gccgtcaagc tggcgttcca gggcctcgcg caggcgcgtc tgggagagcg ggtcggcgag
     1681 cggacgctgg gcgtcgtccg tgatcaggaa gatgtcctcg gcgcgcgcgc cgatggttgc
     1741 gatgcgggcg ttgtgcacgc ggatgccgca gtgcatgaag gcgtggccga tgcggcagag
     1801 caggccgggc cggtcggtgg tgatcagcgt cagcaccgtg tgccgctgcg cggcgtcctc
     1861 ggcgaagtag acggtggtcg gcgtgcggaa atgccgaagc gcgcgcggcg cgcgacgcga
     1921 gggcggcgcg agcggcgctt ccgggtgctg cagctgccag cgcaggcgct gcatgatctc
     1981 gcgcatg
//
LOCUS       DAOQJH010000591         1984 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_46756_length_1984_cov_3.844479, whole genome shotgun sequence.
ACCESSION   DAOQJH010000591 DAOQJH010000000
VERSION     DAOQJH010000591.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1984)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1984)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1984
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46756_length_1984_cov_3.844479"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            4..1014
                     /locus_tag="PLQ18_12120"
     CDS             4..1014
                     /locus_tag="PLQ18_12120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012947933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNE02442.1"
                     /translation="MLPKPYVGWRPRIAIACQGGGSHTAFTAGVLQQLLAAAGADGRF
                     TIAGFSGSSGGAICALLAWYGLLLGSPAEGVRLLEDFWHDNAAETLPDQLLNEFVLGS
                     ARLRDHLLLPELSPYDVPNWGQDQLRSLLEWHVEFERIPQLTGPGRPSLFVGAVDVLA
                     GCARSFRDAEITVATLLASTATPTLFRAVRVGAGHYWDGLFTQNPPIRELAALAPDEL
                     WVVQINPRSTPELPRTLDAIRDRHGELAGHLALEQALGTIELVNRLLASGELAPGRFR
                     PIRVHRLQLARTLDHAAKRDRNPAFLRGLMREGRQVAAAFLEGWSGDLRKPVAADREL
                     SA"
     gene            1076..1354
                     /locus_tag="PLQ18_12125"
     CDS             1076..1354
                     /locus_tag="PLQ18_12125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012534781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HNE02443.1"
                     /translation="MIQSFHHKGLRKFYESGSVAGIQAQHARRLRILLAALDTAETID
                     DMDVPGFRLHALKGRDQDRWSIRVDGNWRVTFEFVNGHARVLDYEDYH"
     gene            1354..1647
                     /locus_tag="PLQ18_12130"
     CDS             1354..1647
                     /locus_tag="PLQ18_12130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014996637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HNE02444.1"
                     /translation="MVMHDPPHPGEFIREVYLEPFGITVRRLAEILQVSPSTLSRILN
                     GSSSLSSEMALRLSKALGRSAESWLAMQAHHDLWQARQRVDLEPVHRIEFHDA"
     gene            1799..>1984
                     /locus_tag="PLQ18_12135"
     CDS             1799..>1984
                     /locus_tag="PLQ18_12135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018869445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HNE02445.1"
                     /translation="MVDFAKEVLPVNLEDEMKQSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLHAMRELGNDWNRP"
BASE COUNT          297 a          673 c          679 g          335 t
ORIGIN      
        1 tcggtgttgc cgaagccgta cgtcgggtgg aggccgcgca tcgcgatcgc ctgccagggc
       61 ggcggcagtc acacggcgtt cacggccggc gtgctgcagc agctgctggc ggcggccggg
      121 gcggacgggc gcttcacgat cgccgggttt tccggcagct cgggcggggc gatctgcgcg
      181 ctgctcgcct ggtacgggct gctgctcggc tcgccggccg agggcgtgcg cctgctggag
      241 gacttctggc acgacaacgc cgccgagacg ctgccggatc agctgctcaa cgagttcgtg
      301 ctcggcagcg cgcggctgcg cgatcacctg ctgctgccgg agctgtcgcc gtacgacgtg
      361 ccgaactggg gccaggacca gctgcgttcg ctgctcgaat ggcacgtcga gttcgagcgc
      421 atcccgcagc tcaccggccc cggccggccg agcctgttcg tcggcgccgt cgacgtgctc
      481 gccggctgcg cacgcagctt ccgcgacgcc gagatcacgg tggcgacgct gctcgcctcg
      541 acggcgacgc cgacgctgtt ccgcgccgtg cgcgtcgggg ccggtcatta ctgggacggc
      601 ctgttcacgc agaacccgcc gatccgcgaa ctcgcggcgc tcgcgcccga cgagctgtgg
      661 gtcgtgcaga tcaacccgcg cagcacgccg gagctgccgc gcacgctcga tgcgatccgc
      721 gaccgccacg gcgagcttgc cggccatctg gcgctggagc aggcgctcgg cacgatcgag
      781 ctggtgaacc gcctgctcgc gagcggcgaa ctcgccccgg ggcgcttccg cccgatccgc
      841 gtccaccggc tgcagctggc gcgcacgctc gatcacgccg ccaagcgcga ccgcaacccg
      901 gccttcctgc gcgggctgat gcgcgaggga cggcaggtgg cggcggcgtt tctggagggc
      961 tggagcgggg acctccggaa acccgttgca gcggatcgcg agctgtctgc ctgatgcatc
     1021 ggatgaatcc ggattcgcgg ttgttgcgtg atacgcaaca tgctaggttc cggccatgat
     1081 tcaatccttt caccacaagg gactcaggaa gttctacgag tccggttcgg tggccggaat
     1141 acaggcgcag catgcacggc ggctccggat tctgctggcc gccctggata ccgccgaaac
     1201 cattgatgac atggatgttc ccggtttccg tttgcatgca ctgaaaggca gggaccagga
     1261 ccgctggtcg atccgggtgg atggcaactg gcgtgtgacc ttcgaattcg tgaacggcca
     1321 cgcccgtgtt ctcgattacg aggattatca ctgatggtca tgcatgatcc gccgcatccg
     1381 ggcgaattca tccgggaggt ttatcttgaa ccattcggca tcacggtccg ccggcttgcc
     1441 gaaatcctgc aggtgtctcc gtcgacgctg agccggatac tgaacggcag cagcagcctc
     1501 agttccgaaa tggccctgcg cctgtccaag gcgctggggc ggtctgcgga aagctggctg
     1561 gcgatgcagg cgcatcatga tctctggcag gcccgccagc gcgtcgatct cgaaccggta
     1621 cacaggatcg aattccacga cgcctgatcc tccgggccgg gccttctggc gtcccgccgt
     1681 ttgtccgggc cactgaccga ccgaccggcc gcgggccggg gccggcatgg tatcctcgcg
     1741 cgcttcctgc aggtgcagcc ccgcggctgc ccgtcccccg acagagctgc cccgtaacat
     1801 ggtcgatttc gcgaaggaag tcctgccggt caatctcgaa gacgagatga agcagtcgta
     1861 tctcgattac gcgatgagcg tgatcgtcgg ccgcgcgctg cccgacgtcc gcgacggcct
     1921 caagcccgtg caccggcgcg tgctgcacgc gatgcgcgag ctcggcaacg actggaaccg
     1981 gccg
//
LOCUS       DAOQJH010000592         1983 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_46808_length_1983_cov_4.822614, whole genome shotgun sequence.
ACCESSION   DAOQJH010000592 DAOQJH010000000
VERSION     DAOQJH010000592.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1983)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1983)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1983
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46808_length_1983_cov_4.822614"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..431
                     /gene="prpE"
                     /locus_tag="PLQ18_12140"
                     /gene_synonym="yahU"
     CDS             <1..431
                     /gene="prpE"
                     /locus_tag="PLQ18_12140"
                     /gene_synonym="yahU"
                     /EC_number="6.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012697532.1"
                     /note="catalyzes the formation of propionyl-CoA using
                     propionate as a substrate; PrpE from Ralstonia
                     solanacearum can produce acetyl-, propionyl-, butyryl- and
                     acrylyl-coenzyme A, and Salmonella enterica produces
                     propionyl- and butyryl-coenzyme A; not expressed in
                     Escherichia coli when grown on propionate/minimal media;
                     ATP-dependent; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="propionyl-CoA synthetase"
                     /protein_id="HNE02446.1"
                     /translation="YFILGRTDDVINVAGHRLGTREIEEAVQAHAAIAEVAVVGIADQ
                     LKGQEAVVFATLKNPALVETEEGRAKLEKEVLAKVVELLGPVARPKHAYFCQTLPKTR
                     SGKLLRRSIQALAEGRDPGDLSTLDDPNSLDEVRRAVGRS"
     gene            825..1712
                     /gene="prpB"
                     /locus_tag="PLQ18_12145"
     CDS             825..1712
                     /gene="prpB"
                     /locus_tag="PLQ18_12145"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003779818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HNE02447.1"
                     /translation="MSELKSAGARFRAAVREERPLQVVGAVNAYFARLAEHSGYKALY
                     LSGGGVAACSCGIPDLGITTMEDVLIDARRITDVTDLPLLVDIDTGWGGAFNIARTVR
                     ELTRAGVAAVHIEDQVAQKRCGHRPNKAIVSQAEMVDRVKAAVDAKTDSNFVIMARTD
                     ALAVEGLQSAIDRACACVEAGADMIFPEAMTDLGMYRQFAEAVGVPVLANITEFGSTP
                     LFTTDELGANGVSLVLYPLSAFRAASKAALNVYQQLRASGTQQGVVDTMQTRAELYEH
                     LGYHAYEQKLDALFSKDAK"
     gene            1746..>1983
                     /locus_tag="PLQ18_12150"
     CDS             1746..>1983
                     /locus_tag="PLQ18_12150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012697778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HNE02448.1"
                     /translation="MTESTQVLPKAKKSVALSGTAAGNTAICTVGRTGNDLNYRGYDI
                     LDLADKCEFEEIAHLLIHHKLPTASELKAYKAKLK"
BASE COUNT          328 a          698 c          670 g          287 t
ORIGIN      
        1 actacttcat cctcgggcgc accgacgacg tcatcaacgt cgccggccac cgcctcggca
       61 cccgcgagat cgaggaagcc gtgcaggcgc acgcggcgat cgccgaggtc gccgtggtcg
      121 gcatcgccga tcagctcaag ggtcaggagg ccgtcgtgtt cgcgacgctg aagaacccgg
      181 cgctggtcga gaccgaggaa ggtcgcgcga agctcgaaaa ggaagtgctc gccaaggtcg
      241 tcgagctgct cggcccggtg gcgcgtccga agcacgccta cttctgccag acgctgccga
      301 agacccgctc cggcaagctg ctgcgccgct cgatccaggc gctggccgaa ggccgcgacc
      361 cgggtgacct gtcgacgctc gacgatccga actcgctcga cgaggtccgg cgcgcggtcg
      421 gtcgcagctg aggccgcggg gcggcgcgcg gcggatcgca tcctgccgcg cgccaccgcc
      481 cccgggccgc gcatcacccg gcccatcagc agacatcttt cagacatctt cagacatctt
      541 gaggggggcc tgcaccgggg acgacggtgc gggttggcag tacgcggccg gaggtcgggc
      601 gatgccgggg ggcgtcgcct tgcatgctgg acggccgtgc agggtccgtg atgccgggca
      661 taaccggtgc acgggccggc ggcggggcgc cggaggtgcc agccgtgggg aacggtgcgc
      721 aagcgcgccg tggcggtccc ccgtccgggg gggcggggga ccgttttccg atgttcatcc
      781 ggcggcgcgc gatccgtgcc gcctgccatc cgaggtcaag cgaaatgagt gagctgaagt
      841 ccgccggtgc acgtttccgt gccgccgttc gtgaggagcg cccgttgcag gtggtcgggg
      901 cggtcaacgc ctatttcgcc cggctcgccg aacactcggg ctacaaggcc ctgtatctgt
      961 ccggcggtgg tgtcgcggcc tgctcctgcg gcatcccgga cctcggcatc acgacgatgg
     1021 aagacgtgct gatcgatgcg cggcgcatca ccgatgtcac cgacctgccg ctgctcgtcg
     1081 acatcgacac cggctggggc ggcgccttca acatcgcccg caccgtgcgc gagctgaccc
     1141 gcgccggcgt cgccgccgtg cacatcgagg accaggtcgc gcagaagcgc tgcggtcacc
     1201 gcccgaacaa ggccatcgtc tcgcaggccg agatggtcga tcgcgtcaag gccgcggtcg
     1261 atgccaagac cgacagcaac ttcgtgatca tggcccgtac cgacgcgctc gccgtcgagg
     1321 gcctgcagtc ggcgatcgac cgtgcctgcg cctgcgtcga ggccggtgcc gacatgatct
     1381 tcccggaggc gatgaccgat ctcggcatgt accgccagtt cgccgaggcc gtcggcgtgc
     1441 cggtgctcgc caacatcacc gagttcggct cgacgccgct gttcacgacc gacgagctcg
     1501 gcgccaacgg cgtcagcctc gtgctctacc cgctgtcggc cttccgtgcg gcttcgaagg
     1561 cggcgctgaa cgtctatcag cagctgcgtg ccagcggcac ccagcagggc gtcgtcgaca
     1621 cgatgcagac ccgcgccgaa ctctacgagc acctcggcta ccacgcctac gagcagaagc
     1681 tcgacgcgct gttcagcaaa gacgccaagt aaagccgcgc tcaaccgaca tcggagtccc
     1741 cagccatgac cgaatccacg caggtcctgc ccaaggccaa gaagtccgtc gcgctgtccg
     1801 gcaccgccgc cggcaacacc gcgatctgca ccgtcgggcg caccggcaat gacctgaact
     1861 accgcggtta cgacatcctc gatctggccg acaagtgcga gttcgaggag atcgcgcacc
     1921 tgctgatcca tcacaagctg ccgaccgcga gcgagctgaa ggcctacaag gccaagctca
     1981 agg
//
LOCUS       DAOQJH010000593         1977 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_47070_length_1977_cov_4.133715, whole genome shotgun sequence.
ACCESSION   DAOQJH010000593 DAOQJH010000000
VERSION     DAOQJH010000593.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1977
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47070_length_1977_cov_4.133715"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..922
                     /locus_tag="PLQ18_12155"
     CDS             <1..922
                     /locus_tag="PLQ18_12155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HNE02449.1"
                     /translation="MAANVLGLDNAATPLGLQAMRELQSLNPQPQRASDAQILFLVIN
                     ASSVTLFPLAVFTYRAQLGAADPTDVFLPILIATGCSTLAGLIACACWQRLALWQPVV
                     LAYLGGIGLLLAALVAWVAGEPAGMAERSALLANGALLALIGAFVAAAAWRRLDAFSV
                     FIEGAQEGFTTAVKIIPYLVAMLVAIGLLRASGVLGLVLDALRALVGDFGFDARWVDA
                     VPVMLLKPLSGSGARGMMIETMQAHGVDSFAGRLACIVQGSTETTFYVLAVYFGSVGV
                     RITRHALACALIADAAGFFAAVTVAYAFFG"
     gene            983..1474
                     /locus_tag="PLQ18_12160"
     CDS             983..1474
                     /locus_tag="PLQ18_12160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02450.1"
                     /translation="MTETERNGWYTLNPHCDLQIEHGVPVRIACEPGNVTANRPALAE
                     DVQRYTGLHVELGPWQAGERGTTREAALQVAAEDFDDVLARYAHASAATYWDRYQQPV
                     HARTLDDFETEAYALDFVTAMHHCGLDWRDVDKHAHSAGWQRALYSEAQRLAAYAELP
                     AQP"
     gene            1777..>1977
                     /locus_tag="PLQ18_12165"
     CDS             1777..>1977
                     /locus_tag="PLQ18_12165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02451.1"
                     /translation="MPPKYKTHRRIHGLAAVIAGLLLCSAAGAEGSHEVAARKFVEGT
                     LRAWMADAVVVETLKAQNAGHAA"
BASE COUNT          264 a          703 c          682 g          328 t
ORIGIN      
        1 catggcggcc aacgtgctcg gcctcgacaa cgccgccacc ccgctcggcc tgcaggcgat
       61 gcgcgagctg cagtcgctga atccgcagcc gcagcgcgcg agcgatgcgc agatcctgtt
      121 cctcgtcatc aacgcctcca gcgtcacgct gtttccgctg gcggtgttca cgtaccgcgc
      181 ccagctcggg gccgccgatc cgaccgacgt gttcctgccg atcctgatcg cgaccggctg
      241 ttcgacgctg gccgggctga tcgcctgcgc ctgctggcag cgtctggcgc tgtggcagcc
      301 ggtggtgctc gcgtatctcg gcggcatcgg cctgctgctc gccgcgcttg tggcctgggt
      361 cgccggcgag ccggccggca tggcggagcg ctcggcgctg ctcgccaacg gcgcgctgct
      421 cgcgctgatc ggcgccttcg tcgccgcggc cgcgtggcgg cggctcgatg cgttctcggt
      481 gttcatcgag ggcgcgcagg agggcttcac gaccgccgtg aagatcatcc cgtacctggt
      541 cgcgatgctg gtggcgatcg gcctgctgcg cgcgagcggc gtgctcggcc tcgtgctcga
      601 tgcgctgcgc gcgctggtcg gcgacttcgg cttcgatgcg cgctgggtcg atgcggtgcc
      661 ggtgatgctg ctcaagcccc tgtccggcag cggcgcgcgc ggcatgatga tcgagaccat
      721 gcaggcgcac ggcgtcgaca gcttcgccgg gcggctcgcc tgcatcgtgc agggctcgac
      781 cgagacgacc ttctacgtgc tcgcggtcta cttcggctcg gtcggcgtgc gcatcacgcg
      841 ccacgcgctc gcctgcgcgc tgatcgccga tgccgccgga ttcttcgctg cggtgaccgt
      901 ggcctacgcg tttttcggct gaggtgcttg acttgtcggc ggcggaccct ggagtccgcc
      961 gcccactgaa ggagtggcca ccatgaccga aaccgaacgc aacggctggt acaccctgaa
     1021 cccgcactgc gacctgcaga tcgagcacgg cgtgccggtg cgcatcgcct gcgagccggg
     1081 caacgtcacg gcgaaccggc cggccctggc cgaggacgtg cagcgctaca ccgggctgca
     1141 cgtcgaactc ggcccctggc aggccggcga gcgcggcacg acgcgcgagg cggcgctgca
     1201 ggtcgccgcc gaggacttcg atgacgtgct cgcgcgctac gcgcatgcct cggccgcgac
     1261 ttactgggat cgctatcagc agccggtgca cgcccgcacg ctcgacgact tcgagaccga
     1321 ggcctacgcg ctcgatttcg tcaccgcgat gcaccactgc ggtctcgact ggcgcgatgt
     1381 cgacaagcac gcccacagcg ccggctggca gcgggcgctg tacagcgagg cgcaacgcct
     1441 cgccgcgtac gcggaactgc ccgcgcaacc gtgatcttgt gtaaaaaaac ctgactgtga
     1501 tcgcggtggc ggctacggtc gccgccggga tcccgctttc cggcagcggc gaatccacgg
     1561 ctcaagtgac ggttccgtaa atcctttctg ctgccggccg gttgcgcttc cgcagccggt
     1621 cccgccctgc gcgtgccggg agcactcgcg gccgctcgcc cttcacagtc gcgccgggct
     1681 gccgatgatg gttctgatcg tccgccctgc tgcggccggt gatctgcctg cccataacga
     1741 aagtctccgg agggatccgg gaggaaggag aacgccatgc ccccgaagta caagacccat
     1801 cgccgtattc atggccttgc tgccgtcatc gccggcctgc tgctgtgctc cgcggccggc
     1861 gccgaaggca gccatgaggt tgccgcccgc aagttcgtcg aaggcacgct gcgcgcgtgg
     1921 atggccgatg cggtcgtcgt cgagacgctg aaggcgcaga acgccggaca cgcggcg
//
LOCUS       DAOQJH010000594         1975 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_47188_length_1975_cov_2.833854, whole genome shotgun sequence.
ACCESSION   DAOQJH010000594 DAOQJH010000000
VERSION     DAOQJH010000594.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1975)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1975)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1975
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47188_length_1975_cov_2.833854"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="PLQ18_12170"
     CDS             <1..270
                     /locus_tag="PLQ18_12170"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNE02452.1"
                     /translation="VAVAERIRALLAELRVATGSGGVVALSGSVGVARCPAGERRELE
                     DLLRAADRALYAAKHAGRDRVVTEDAANSDEAPVQPDGSSTDNAA"
     gene            complement(234..653)
                     /locus_tag="PLQ18_12175"
     CDS             complement(234..653)
                     /locus_tag="PLQ18_12175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018233173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HNE02453.1"
                     /translation="MSSGLWSLFAGSFLAATLLPGGSELLYAALLLRGEDSPLALLAV
                     ATLGNTLGGLSSFAIGRWLRQRRPAGPFAARHEQALTRLRRFGAPALLASWVPVIGDP
                     LCVAAGWLGIGWTAATLWIALGKAARYAALSVLLPSG"
     gene            complement(693..1382)
                     /locus_tag="PLQ18_12180"
     CDS             complement(693..1382)
                     /locus_tag="PLQ18_12180"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HNE02454.1"
                     /translation="MKLPPLPPYRVREHARARRVRLRVSPAHGIEVVVPSGFPARELP
                     RLLEPHRHWMEMQLARCRAGLELPAVMLLPALGETWSLRHEALPEGRRARLLVEAGAL
                     HLQAADAAAARALLQRFVQRRAAEVLPPWLARVAEETGLRFAGVRIRTQRARWGSCSA
                     SGRISLNCKLLFLEPALVRHVLVHELCHTVHLNHSPAFWKVVADHDPDWRRLRAELQA
                     RPAMAPAWIEA"
     gene            complement(1379..1864)
                     /locus_tag="PLQ18_12185"
     CDS             complement(1379..1864)
                     /locus_tag="PLQ18_12185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012973549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HNE02455.1"
                     /translation="MPAPLPPYHCTVEADWTDYNGHLNVAFYVLVFDRATDAALAACG
                     LDARLRERSGSTVFAAECHVSYQRELPGGAPLAVDTQILGLDDKRVHLFHTLGHAEAG
                     WPAASAEFLLLHVDLGSRRVAPWPPAVHARLAAVRSVHERLPVPAQAGRSVQLRRREQ
                     T"
BASE COUNT          284 a          741 c          722 g          228 t
ORIGIN      
        1 gtcgcggtcg ccgagcgcat ccgcgcgctg ctcgccgagc tgcgcgtcgc caccggcagc
       61 ggcggtgtcg tggcgctgag cggcagcgtc ggcgtcgcgc gctgcccggc cggcgaacgg
      121 cgcgagctcg aagacctgct gcgcgccgcc gaccgcgcgc tgtacgccgc caagcacgcc
      181 gggcgcgacc gcgtcgtcac cgaggacgcc gcgaacagcg acgaggcccc ggttcagccc
      241 gacggcagca gcaccgacaa cgccgcataa cgcgcggcct tgccgagcgc gatccacagc
      301 gtcgccgccg tccagccgat gccgagccag ccggcggcga cgcacagcgg atcgccgatc
      361 accggcaccc acgaagccag cagggccggt gcgccgaagc ggcgcagccg cgtcagcgcc
      421 tgttcgtggc gggccgcgaa gggcccggcc gggcgccgct gccgcagcca gcggccgatc
      481 gcaaacgagc tgagcccccc gagcgtgttg ccgagcgtcg ccaccgccag cagcgccagc
      541 ggcgaatcct cgccgcgcag cagcagcgcc gcgtacagca gttccgagcc tccgggcagc
      601 agcgtcgcgg ccaggaagct gccggcgaac aggctccaga gcccgctgct catcccgtcc
      661 cccattccct cactcctccc gccacgctcc cgtcaggctt cgatccaggc cggcgccatc
      721 gccggtcgtg cctgcagttc ggcgcgcagc cggcgccagt ccggatcgtg gtctgcgacc
      781 accttccaga acgccgggga atggttcagg tgcacggtgt ggcagagctc gtgcaccagc
      841 acgtgccgca ccagcgccgg ttcgaggaac agcagcttgc agttcaggct gatgcgaccg
      901 ctggccgagc acgagcccca gcgcgcccgc tgcgtacgga tgcgcacccc ggcaaagcgc
      961 aggccggttt cctcggcgac gcgtgcaagc cacggcggca ggacttcggc ggcgcgccgc
     1021 tgcacgaagc gctgcagcag cgcgcgggct gcggccgcgt ccgccgcctg caggtgcagg
     1081 gcgccggctt cgacgagcag ccgggcacgc cgtccttcag ggagggcttc gtggcgcagc
     1141 gaccaggttt cgccgagcgc cggcagcagc atcaccgccg gcagttcgag cccggcgcgg
     1201 cagcgtgcga gttgcatctc catccagtgg cggtgcggct ccagcagccg cggcagctcg
     1261 cgcgccggaa agccgctcgg cacgacgacc tcgatgccgt gcgccggcga cacgcgcagg
     1321 cgcacgcgcc gggcgcgggc gtgctcgcgg acccggtacg ggggcagcgg cggcagcttc
     1381 atgtctgctc gcgccggcgc aactgcaccg aacgcccggc ctgcgctggc accggcagcc
     1441 gttcatggac gctgcgcacc gcggcaagcc gtgcgtgtac cgccggcggc cacggcgcga
     1501 cccggcggct gccgagatcg acgtgcagca gcaggaactc ggcactcgcc gccggccagc
     1561 cggcttcggc gtggccgagc gtgtggaaca gatgcacgcg cttgtcgtcg aggccgagga
     1621 tctgcgtgtc gactgcgagc ggcgcgccgc ccggcagctc gcgctgatag ctgacgtggc
     1681 attcggccgc gaacaccgtg ctgccgctgc gctcgcgcaa ccgcgcatcg agtccgcagg
     1741 cggcgagggc ggcgtcggtg gcgcggtcga acaccagcac gtagaaggcg acgttcaggt
     1801 ggccgttgta atcggtccag tccgcttcga cggtgcagtg atacggcggc agcggcgcgg
     1861 gcatcggcgg ctcagatccc gagcgcgcgc cagtccggcc ggccgtcgag cagcggcggg
     1921 caccagtagt gcgcgccgtt cagcggccgt gtgaagttga acagcgcgtc atgga
//
LOCUS       DAOQJH010000595         1967 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_47578_length_1967_cov_3.532427, whole genome shotgun sequence.
ACCESSION   DAOQJH010000595 DAOQJH010000000
VERSION     DAOQJH010000595.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1967)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1967)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1967
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47578_length_1967_cov_3.532427"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /locus_tag="PLQ18_12190"
     CDS             <1..282
                     /locus_tag="PLQ18_12190"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: protein motif:HMM:NF014685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HNE02456.1"
                     /translation="IALPPARPDGLAAAVSAALPRRRGRLLVFVLLLVLLCSDTPGQA
                     AGLAGLAGAWFWGRHQRARLGGCTGDTLGAGCELLEAAVLLAIFVALAQ"
     gene            684..1370
                     /locus_tag="PLQ18_12195"
     CDS             684..1370
                     /locus_tag="PLQ18_12195"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HNE02457.1"
                     /translation="MHPFPRTDREVPVGIRQTVSQHPRQPLGTAILKSLAVFGLPCCL
                     ALSLVLAPARTHAAEDEAVRSRPLVSSLLDDDGSERLYGTRIQKLADYIEENFAVDEA
                     KAYSIVTHAIEHGTKNGLKPELLLAVIAVESTFKEDAVSSQGARGLMQVMPRFHEDAI
                     REIGGAHALFKPGPNIRTGTGILVEYLDRANGDLHKALARYNGSSGKRSKVYANKVLS
                     MYRKFQLASS"
     repeat_region   1568..1966
                     /inference="COORDINATES: alignment:crt:1.2"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=1641..1676
                     /rpt_unit_seq="gcttcaatgaggccgcggcattacaccgcggaaaac"
BASE COUNT          357 a          678 c          613 g          319 t
ORIGIN      
        1 atcgcgctgc cgccggcgcg ccccgacgga ctcgccgcag cggtgtcggc agccctgccg
       61 cgccggcgcg ggcgcctgct ggtgttcgtg ctgctgctcg tgctgctttg cagcgacacg
      121 cccgggcagg cggccggtct tgccgggctt gccggcgcgt ggttctgggg ccggcaccag
      181 cgcgcacggc tcggcggctg caccggtgac acgcttggcg ctggctgcga gctgctggaa
      241 gccgcggtgc tgctggcgat atttgttgcg ttggcacaat aacaacaggc agccacgggc
      301 ttgcgcgggc cctgtccgga aagttgcgcc tgctggcaag agccaatcgt taaaacccgt
      361 aaggatcaga gatttgtatc gagccctcac aaggcttgag gacactgttg ttttgtttga
      421 cgggcacaac agtgcttgtc cggtaatcgg ggtgtccgta ccctgcacgc aatttcacgc
      481 catgaaaccg gaggtagcgc ctatcgatac agccgagaag aggctggcaa gtctttgatc
      541 cttcagcaaa agaaggaaga agtctgccag cggccgggtc cggaccggac acgtcaagcg
      601 cggcatgcgc gacgtttccc ccgccgatcc gccggccgcc ccgggcaacc ccctcgcact
      661 ggtcagaaaa caggagcaat cccatgcacc ctttcccccg gacggatcga gaagtaccgg
      721 tcggcatccg ccagaccgtt tcgcagcacc cgcggcagcc gcttggcacc gcgatcctga
      781 agtccctggc agttttcggc ctgccctgct gtctggcact cagcctggtg ctggccccgg
      841 cgcgcacgca tgcggccgag gacgaagccg tgcgcagccg cccgctggtc tccagcctgc
      901 tggacgatga cggcagcgag cgcctctacg gcacgcgcat ccagaagctc gccgattaca
      961 tcgaggagaa cttcgccgtc gacgaggcga aggcgtattc catcgtcacg cacgcgatcg
     1021 agcacggcac gaagaacggc ctgaagcccg agctgctgct cgcggtgatc gcggtcgaat
     1081 cgaccttcaa ggaagacgcc gtcagcagtc agggcgcgcg cggcctgatg caggtgatgc
     1141 cgcgcttcca cgaggatgcg atccgcgaga tcggcggcgc acacgcgctg ttcaagcccg
     1201 gcccgaacat ccgcaccggc accggcatcc tggtcgagta cctcgaccgc gccaacggcg
     1261 atctgcacaa ggcgctggcc cgttacaacg gcagctccgg caagcgttcg aaggtgtacg
     1321 cgaacaaggt gctgtcgatg taccgcaagt tccagctcgc ctcgtcctga ggccgcaacc
     1381 atcctccgcc acgaccgtca ggggccacgc agcccctgcg gttcaaacca tttccttccg
     1441 acgccgccgg gcgcacccgc cccggccgcc gtcactgccc gccagccgcg acctgatgca
     1501 cggccttcgt gaggcaacgc gcattgcccg gcctcgtcga tatcgggctt gaagcgaaat
     1561 cggctttggc gcaatgaggc cgcggcatta caccgcggaa aaccgcatgt ggacttcgtg
     1621 cagcacgagg taggccctcc gcttcaatga ggccgcggca ttacaccgcg gaaaactgcc
     1681 gtcgacgcac aggtctgtct tgccccagat ttcgcttcaa tgaggccgcg gcattacacc
     1741 gcggaaaacg ttgcggccgt tccagccgag ccggcagccg acgtgcttca atgaggccgc
     1801 ggcattacac cgcggaaaac ctcgccggca ccgccaccgt ctggatcgag atcgtcgctt
     1861 caatgaggcc gcggcattac accgcggaaa acgcgtactt ttccgccgcg cccatcgtgc
     1921 gatcaccacg gcttcaatga ggccgcggca ttacaccgcg gaaaacc
//
LOCUS       DAOQJH010000596         1962 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_47829_length_1962_cov_3.289984, whole genome shotgun sequence.
ACCESSION   DAOQJH010000596 DAOQJH010000000
VERSION     DAOQJH010000596.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1962)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1962)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1962
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47829_length_1962_cov_3.289984"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            290..1027
                     /locus_tag="PLQ18_12200"
     CDS             290..1027
                     /locus_tag="PLQ18_12200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02458.1"
                     /translation="MNDARLADLREQAVRLGYGLQTMLPVSAISAPGLASLAAPFRTL
                     WLVCNTGDAVQRRWRAGRDIEALLADNAATGAGSCGPLDDFCRAELGALLGTRAHRVL
                     HPHPASTAPLPLLALLAAAGWLRPGPFMNTLHARYGSWWAVRAVIALDAGADAPGEAL
                     GPSPCLDCSAPCVPACPAAAVGKPGWEAQRCLAQRVTIGSGCARRCIARESCPAGREW
                     RYEPATIAYHYGASQQTLQRWRSGERA"
     gene            1050..1337
                     /locus_tag="PLQ18_12205"
     CDS             1050..1337
                     /locus_tag="PLQ18_12205"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HNE02459.1"
                     /translation="MLTVIARIEARPEFAACLKQAMLALLAPTRAEPGCLAYELLESS
                     DDPALIFFHERWADRDALEAHWQSAHLLAYRDATKGWIDRRELTVLRPVEP"
     gene            1375..1653
                     /locus_tag="PLQ18_12210"
     CDS             1375..1653
                     /locus_tag="PLQ18_12210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002737686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HNE02460.1"
                     /translation="MDFEWDETKALANLRKHGVSFAEASTLFGDPLELTIADPDHSHG
                     EFRFLSLGRSHAGRLLVVAYTERDHRIRIISARPATSREIQCHEAGRF"
     gene            1634..1864
                     /locus_tag="PLQ18_12215"
     CDS             1634..1864
                     /locus_tag="PLQ18_12215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008312757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02461.1"
                     /translation="MKPADSDTDTELRDEYDFSAAIRGRHHSDYQRGTNLVLLDADVA
                     AAFPDAAAVNNALRLLLRLARQQLPPVPKPSE"
BASE COUNT          292 a          719 c          644 g          307 t
ORIGIN      
        1 ctggatccac tgccgcggcg tgccgcggct ccagtgcgcg atgacatcgg cctggtccgc
       61 atccggtgtc ggcgcggaag ctccggaaca gggcatcggg tggttcatgg cgggcaggct
      121 ccatgcagat ccgggaaggc ccgaagtccg ggcatcgggc ggccgggcgt cgcaccgtat
      181 cctgtctgca agcgtgccga cccgctgcgt ccgctgctgc atcgatggtt gacaatccgg
      241 acccgcacac cggtgatggc gattcccgtc gccgccgcca tgaacccgca tgaacgacgc
      301 ccgccttgcc gatctgcgtg aacaggccgt ccggcttgga tacggcctgc agaccatgct
      361 gccggtttcc gccatctccg cccccggtct cgcctcgctc gcggccccgt tccgaacgct
      421 gtggctggtc tgcaataccg gcgatgccgt gcagcggcgc tggcgcgccg ggcgcgacat
      481 cgaggcgctg cttgctgaca acgccgcgac cggcgccggt tcctgcggcc cgctcgatga
      541 cttctgccgc gccgaactcg gcgccctgct cggcacacgg gcgcaccgcg tgctgcatcc
      601 gcatccggcc tcgaccgcgc cgctgccgct gctcgcactg ctcgccgcgg ccggctggct
      661 gcggccggga ccgttcatga acacgctgca tgcgcgctac ggcagctggt gggcggtgcg
      721 ggccgtgatc gcgctcgatg ccggagccga tgcgccgggc gaagccctcg gcccatcgcc
      781 gtgcctcgac tgcagcgctc cgtgcgtccc tgcctgcccg gcggcagcgg tcggcaaacc
      841 gggctgggag gcgcagcgct gtctcgcaca gcgcgttacg atcggcagcg gatgtgcccg
      901 acgctgcatc gcgcgcgaaa gttgtccggc aggccgggaa tggcgttacg agccggccac
      961 gatcgcctat cactacggtg catcgcagca aacactgcag cgctggcgta gcggggagcg
     1021 cgcctgacca accgcccgag gggatcgcca tgctgaccgt catcgcccgc atcgaagccc
     1081 gccccgaatt cgccgcctgc ctgaagcagg ccatgctcgc gctgctggcc ccgacccgtg
     1141 ccgaacccgg ctgcctcgcc tacgaactgc tggaaagcag cgacgatccg gcgctgatct
     1201 tcttccacga acgctgggct gaccgggatg ccctcgaagc ccactggcag tcggcgcacc
     1261 tgctcgccta ccgggacgcg accaagggct ggatcgaccg gcgcgagctc accgtgctgc
     1321 ggccggtcga gccttgagcc cgcgaaagcc gcgcagcagc atcgggctgg ctgaatggac
     1381 ttcgaatggg atgaaaccaa ggcgcttgcg aacctgcgca agcatggtgt cagcttcgcg
     1441 gaagcttcga ccttgttcgg tgatccgctg gagctgacga ttgccgaccc ggatcactcg
     1501 cacggcgagt tccggttcct gagcctcggt cgcagccatg ccggacgtct gctggtcgtg
     1561 gcctatacgg aacgggacca ccgcatccgc atcatcagcg cccgccccgc cacttcccgg
     1621 gagatccagt gccatgaagc cggcagattc tgataccgac accgaattgc gcgacgagta
     1681 cgatttttcg gccgccatcc gcggcaggca tcacagcgac tatcagcgcg gcaccaacct
     1741 cgtgttgctg gacgccgatg tcgcggccgc ctttccggat gcggcagccg tcaacaacgc
     1801 cctgcgcctg ctgctgaggc tcgcccggca gcaactgccg cccgtcccca agccttcgga
     1861 atgatgcagg tttgcccggt gacgaacgtc atggtgcgct tcgcgggtca ggccgcgaac
     1921 gccgccccgc aggcaccgct gatcgccgcc caggccccgg cc
//
LOCUS       DAOQJH010000597         1952 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_48305_length_1952_cov_3.081708, whole genome shotgun sequence.
ACCESSION   DAOQJH010000597 DAOQJH010000000
VERSION     DAOQJH010000597.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1952)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1952)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1952
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48305_length_1952_cov_3.081708"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1875)
                     /locus_tag="PLQ18_12220"
     CDS             complement(<1..1875)
                     /locus_tag="PLQ18_12220"
                     /inference="COORDINATES: protein motif:HMM:NF015926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF349 domain-containing protein"
                     /protein_id="HNE02462.1"
                     /translation="MIFTRLFRRAPSWRHRDPRERLRALQSLADGDPALLELAGDSDP
                     GVRRAALDRLHDPLRLLEHLHAEDDAVVREAATGRLRTLLAGHLAGGPAVTVRLAVLA
                     DPRLPPALLVHLAAEAAESALRLAALAGVLDQRQLAVIAVQDAVAEVRLAATERIHDP
                     ELLAGLARSFRARDKRLYRLVSERLETVRRRETERLRAAALLDELDALAARERLEAED
                     VVRAAALEREFGQLPAEADDPARRQAAQARLAPLLAAHQAHGVAVARCLRALDAITAG
                     FAAHGDATACATALAGLEAEAPADPRIVVRMQALRERLAERGRDTAHAEARERWLNAL
                     ETLASIDAATRTRLEAEWQALPAPADATLRRRQDERARARLAQLLAAQPAPAPTQSSA
                     PKPAAPRRAPAEPDAQLLARLETLEAALEAGELHTAQPLYEQIQTEARGLRALDARLH
                     AAAARLAELRDWARWGGRQAREQLCLAAEGLVPDAWAPAELARRVQQLRAQWKQLDGG
                     ELPATRGQWERFNSACERAYAPAQAAFAQAAAEREQHIGARRALIERLQQLDAGTDWS
                     APDWRTLDQAWRELQRGWREAGTVPRNAYRTLEQDWKAAAEAVGGRIEAERSASAPGA
                     R"
BASE COUNT          210 a          697 c          792 g          253 t
ORIGIN      
        1 cctcgcgccg ggcgcgctcg cgctgcgttc ggcctcgatg cgtccgccga cggcctcggc
       61 ggcggccttc cagtcctgct ccagcgtccg gtaggcgttg cgcggcacgg tgccggcctc
      121 gcgccagccg cgctgcagct cgcgccaggc ctgatccagc gtccgccagt ccggggccga
      181 ccagtcggtg ccggcatcga gctgctgcag gcgttcgatc agcgcgcggc gtgcgccgat
      241 gtgctgctcg cgctcggccg cggcctgcgc gaaagcggcc tgcgccgggg cataggcgcg
      301 ttcgcaggcg ctgttgaagc gttcccactg tccgcgcgtc gccggcagct cgccgccgtc
      361 gagctgcttc cactgcgcgc gcagctgctg cacgcggcgc gcgagttcgg ccggtgccca
      421 ggcgtccggc accagccctt cggcggcgag gcagagctgt tcgcgcgcct gccggccgcc
      481 ccagcgcgcc cagtcacgca gttcggcgag ccgggcggcg gcggcgtgca gccgcgcatc
      541 gagggcgcgc aggccgcggg cctcggtctg gatctgctcg tacagcggct gcgccgtatg
      601 cagttcgccg gcttcgaggg cggcttcgag ggtttcgagc cgcgcgagca attgcgcatc
      661 cggttcggcc ggggcacggc gcggcgcggc cggtttcggt gcggaggact gcgttggtgc
      721 cggtgccggc tgtgcggcca gcagctgcgc gagccgggcg cgagcgcgtt cgtcctgacg
      781 gcggcgcagc gtggcatcgg ccggtgccgg gagcgcctgc cattcggctt cgagccgggt
      841 ccgggtcgcg gcgtcgatcg acgccagcgt ttcgagcgcg ttcagccagc gttcgcgggc
      901 ttcggcatgg gcggtgtcgc gtccgcgctc ggccagccgc tcgcgcagcg cctgcatgcg
      961 cacgacgatg cgcggatcgg ccggagcctc ggcttcgagc ccggcgagcg ccgtggcgca
     1021 ggccgtggcg tcgccgtgcg cggcgaagcc ggcggtgatg gcatcgaggg cgcgcaggca
     1081 gcgggcgacc gcgacgccgt gggcctgatg cgcggcgagc agcggcgcga gccgggcctg
     1141 cgcggcctga cggcgggccg ggtcatcggc ttcggccggc agctgcccga attcgcgttc
     1201 gagcgcggcg gcgcggacca cgtcctcggc ttccaggcgc tcgcgggcgg ccagcgcgtc
     1261 gagctcatcg agcagcgccg cggcgcgcag gcgctcggtc tcgcgccggc gcacggtttc
     1321 cagccgctcg ctgacgagcc ggtacaggcg cttgtcgcga gcgcggaagc tgcgcgccag
     1381 cccggcgagc agttcgggat cgtggatgcg ttcggtggcc gcgaggcgca cctcggcaac
     1441 cgcgtcctgc acggcgatga cggcgagctg ccgctgatcg aggacgccgg cgagcgcggc
     1501 cagccgcagc gccgattcgg ccgcctcggc ggcgaggtgg acgagcagcg ccggcggcag
     1561 gcgcggatcg gcaagcacgg cgaggcgcac cgtcaccgcc ggcccgccgg cgaggtgccc
     1621 ggcgagcagc gtgcgcagcc ggccggtggc ggcctcgcgc acgacggcgt cgtcctcggc
     1681 gtgcagatgt tcgagcagcc gcagcggatc gtgcagccgg tcgagcgcgg cgcggcgcac
     1741 gcccggatcg ctgtcaccgg ccagttcgag cagtgccgga tcgccgtcgg cgaggctttg
     1801 cagcgcgcgc aggcgctcgc ggggatcacg atggcgccac gacggcgcac ggcggaacag
     1861 gcgggtgaag atcatcggtg actcggcagg tctcgggcgg gggcgattga agcacaaagc
     1921 ggcgggcgcg tccggagccg gccggttacg gg
//
LOCUS       DAOQJH010000598         1946 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_48608_length_1946_cov_2.562136, whole genome shotgun sequence.
ACCESSION   DAOQJH010000598 DAOQJH010000000
VERSION     DAOQJH010000598.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1946)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1946)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1946
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48608_length_1946_cov_2.562136"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            211..591
                     /gene="arsC"
                     /locus_tag="PLQ18_12225"
     CDS             211..591
                     /gene="arsC"
                     /locus_tag="PLQ18_12225"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018746396.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HNE02463.1"
                     /translation="MSATETPSSKLSFTIYHNPRCSKSRAALDLLSQHGIEPAVVRYL
                     DTPPDAATLAQLLDQLGLEPRALLRSGEDEYKTLNLADPALSREQLIAAMVAHPKLIE
                     RPIVVHDERAVLGRPPERVLELLR"
     gene            588..1193
                     /gene="wrbA"
                     /locus_tag="PLQ18_12230"
     CDS             588..1193
                     /gene="wrbA"
                     /locus_tag="PLQ18_12230"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015280555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HNE02464.1"
                     /translation="MTAPAAEILVLYYSRHGATAEMARLVARGVEEVPGMQARLRTVP
                     AVSAVCEKTADDIPAEGPPYATLADLRECAGLALGSPTRFGNMAAPLKFFLDGTSELW
                     LSGALAGKPAGVFTSTGSLHGGQETTLLTMMLPLLHHGMLIVGLPYTETVLHTTRTGG
                     TPYGPSHFAGTEARPSPDEKTLCRALGRRLAQTAQRLAVPS"
     gene            1190..1561
                     /locus_tag="PLQ18_12235"
     CDS             1190..1561
                     /locus_tag="PLQ18_12235"
                     /inference="COORDINATES: protein motif:HMM:NF021369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2069 domain-containing protein"
                     /protein_id="HNE02465.1"
                     /translation="MIRAARWLTLVCWAGLFLFWLARPIWLVPTEAPAIPLKLLLAGA
                     LLFPLRGLLHGKPYTHGWACFLALGYFVLSVDDLAGGLASKPLGWTGLLLAVGFFFGC
                     MGYARLEGRRRKLAATVAPQP"
     gene            1558..>1946
                     /locus_tag="PLQ18_12240"
     CDS             1558..>1946
                     /locus_tag="PLQ18_12240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02466.1"
                     /translation="MNRSARRPVASCACMVLLVAGRAMADPAACHVTAPVDWQGITVR
                     WEGACANGLAEGSGVLRGLEKGQVVRLFLGRMLAGQPQLGVVEWPTGYVAGRFEAGRV
                     VTSDDRNDTLRAFRAASQAAEAVSERFR"
BASE COUNT          263 a          699 c          678 g          306 t
ORIGIN      
        1 cccggagacg agccggcgcc tgcttcagcc actgacgggc cgggcgctgc aaaggttcga
       61 cgcccggttg cggatcacgg ctgcggcgga tgccgctaag atgccgcgcg gcgccgccgg
      121 ccgaggtcac ggcgacgcca gcctcctccc tgatccggca gcctcagccg gctcccggct
      181 ccgacctcgc agaatccggt ccttccccgc atgtccgcaa ccgaaacgcc ctcgtccaag
      241 ctctcgttca cgatctacca caacccgcgc tgctcgaagt cgcgcgccgc cctcgacctg
      301 ctgagtcagc acggcatcga gccggccgtc gtgcgctatc tggacacgcc gccggatgcc
      361 gccacgctcg cgcagttgct cgatcaactc gggctggagc cgcgcgcgct gctgcgcagc
      421 ggcgaggacg agtacaagac gctgaacctg gctgatccgg ccctgagccg cgagcagctg
      481 attgcggcga tggtcgcgca tccgaagctg atcgagcggc cgatcgtcgt gcatgacgag
      541 cgggccgtgc tcggccgccc gcccgagcgc gtgctggagt tgctgcgatg acggcgccgg
      601 cggccgagat tctggtgctc tattactcgc gccacggtgc cacggccgag atggcgcggc
      661 tggtggcgcg cggcgtcgag gaagtccccg gcatgcaggc gcggctgcgc acggtgccgg
      721 cggtctcggc ggtctgcgag aagaccgccg acgacatccc ggccgaaggt ccaccgtatg
      781 ccacgctcgc cgacctgcgc gaatgcgcgg ggcttgcgct cggcagcccg acgcgcttcg
      841 gcaacatggc ggcaccgctg aagttcttcc tcgatggcac ttcggagctg tggctttccg
      901 gcgcgctcgc cggcaagccg gccggcgtgt tcacgtccac cggcagcctg cacggcggcc
      961 aggagaccac gctgctgacg atgatgctgc cgctgctcca ccacggcatg ctgatcgtcg
     1021 gcctgcccta caccgaaacg gtgctgcaca cgacgcgcac cggcggcacg ccctacggcc
     1081 ccagccactt cgccggcacc gaagcccgcc cgagtcccga cgagaaaacc ctctgccgcg
     1141 ccctcggccg gcggctggcg cagaccgcgc agcgtctggc ggtgccgtca tgatccgcgc
     1201 cgcccgctgg ctgacgctcg tctgctgggc gggcctgttc ctgttctggc tcgcccgccc
     1261 gatctggctg gtgccgaccg aggccccggc gatcccgctc aagctcctgc tcgccggcgc
     1321 cctgctgttt ccgctgcgcg gcctgctgca cggcaagccg tacacgcacg gctgggcgtg
     1381 cttcctcgcg ctcggctatt tcgtgctgtc ggtcgatgat ctcgccggcg ggcttgccag
     1441 caagccgctc ggctggaccg ggctgctgct ggcggtgggg tttttctttg ggtgcatggg
     1501 gtatgcgcgg ctggagggaa ggcggcggaa gctggcggcc accgtggccc cgcagccatg
     1561 aaccgcagcg cccggcggcc ggttgccagt tgtgcctgca tggtgcttct ggtggccggc
     1621 agggcaatgg ccgatcccgc tgcctgccac gtcaccgctc cggtggactg gcagggcatt
     1681 accgtacgct gggaaggagc ttgcgcaaac ggactggccg aaggttccgg cgtcttgcga
     1741 ggcctcgaaa aaggacaggt cgtgcgcctg ttcttgggcc ggatgctggc cggacagccg
     1801 cagctcggtg tcgtcgaatg gccgacgggt tacgtcgccg ggcgcttcga ggccggcagg
     1861 gtggtgacga gcgacgatcg caacgacacc cttcgggcat tccgcgcagc cagccaggca
     1921 gccgaagcgg tcagcgagcg tttccg
//
LOCUS       DAOQJH010000599         1935 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_49103_length_1935_cov_3.383511, whole genome shotgun sequence.
ACCESSION   DAOQJH010000599 DAOQJH010000000
VERSION     DAOQJH010000599.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1935)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1935)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1935
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49103_length_1935_cov_3.383511"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="PLQ18_12245"
     CDS             complement(<1..272)
                     /locus_tag="PLQ18_12245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012978403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HNE02467.1"
                     /translation="MTMWTPDLNPYAGPRYRAIADALEADLREGRLAPGARLPTHREL
                     AERLGVTVGTITRAYAEAEARGLTRVTVGRGTFVRETRVQAPVMIHA"
     gene            368..604
                     /locus_tag="PLQ18_12250"
     CDS             368..604
                     /locus_tag="PLQ18_12250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012332198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HNE02468.1"
                     /translation="MSTIRELSLPLSRSLPSLRELVALPATGMGRIVRTAGLWYRRHN
                     ERRALAALPDYLLKDIGLSDADVWAESRKWFWQE"
     gene            739..1539
                     /locus_tag="PLQ18_12255"
     CDS             739..1539
                     /locus_tag="PLQ18_12255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015048025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HNE02469.1"
                     /translation="MQLALYQIDAFTDRPFAGNPAAVVPLERWLPDAVLAAIAAENNL
                     SETAFFVPEGEGFRLRWFTPGIEVDLCGHATLASAWVLYERLGWRAARIPFVCASGPL
                     GVRRLEDGRLELDFPARPLLATDAGLAEAVAAALGAQPVEVRSSDTNLFAVFACEADI
                     RALRPDFAALARLPKQGVIVTAPGDHADFVSRYFAPAAGIDEDPVTGSAHCELTPYWA
                     ARLGRDRLHAQQVSKRHGELQVCLDGARVRIAGHAVPYLEGRITLPGD"
     gene            complement(1721..>1935)
                     /locus_tag="PLQ18_12260"
     CDS             complement(1721..>1935)
                     /locus_tag="PLQ18_12260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02470.1"
                     /translation="MSYRIFSTEHQGQVVSREASRPTSSQPFSTQFTAPDQPQAAEYE
                     TLYNAVSVKRHLEAVGVHGLYIREVQ"
BASE COUNT          275 a          696 c          638 g          326 t
ORIGIN      
        1 gcatgaatca tcaccggcgc ctgcacgcgc gtctcgcgca cgaaggtgcc gcgcccgacc
       61 gtcacgcgcg tcagtccgcg cgcttcggcc tcggcatagg cgcgcgtgat cgtgccgacg
      121 gtcacgccga ggcgctcggc cagctcgcgg tgcgtcggca ggcgcgcgcc cggcgcaagc
      181 cgcccctcgc gcaggtcggc ttcgagggca tcggcgatcg cgcggtagcg cgggccggcg
      241 taggggttga ggtctggcgt ccacattgtc atggtgacaa agaatggctt gacatccgta
      301 ttgtgtcaac tactctgcat gtcgcggtga caatgtagtg atcgccccta ctgctggaga
      361 tgcaaaaatg agcacaatcc gcgaattgtc tcttcctctt tcccggtccc tgccttcgct
      421 gcgcgagctc gtcgctctgc cggccacggg aatggggcgc atcgtccgca ccgccggcct
      481 ctggtatcgc cgccacaatg aacgccgcgc cctggcggcc ctgcccgatt acctgctgaa
      541 ggacatcggc ttgagcgatg ccgatgtctg ggccgaatcg cgcaagtggt tctggcagga
      601 atgagcgcgg cgcgcggccg atgcagcggc ctttgcaggc gctgcaggcc gcgggcaggg
      661 tgacaaagca cattgtcacc ctcgatgccg gaaccgcgca tgaccgcccg cacagccctt
      721 gtccccggag ccttcgccat gcagctcgcc ctgtaccaga tcgacgcctt caccgaccgg
      781 cccttcgccg gcaacccggc agcggtcgtg ccgctcgaac gctggctgcc cgatgccgta
      841 ctggccgcga tcgccgccga aaacaacctg tcggaaaccg ccttcttcgt gcccgaaggc
      901 gaaggcttcc ggctgcgctg gttcacgccc ggcatcgagg tcgatctctg cggccatgcc
      961 acgctcgcca gcgcctgggt gctctatgag cgcctcggct ggcgcgcagc gcgcattcct
     1021 ttcgtctgcg ccagcggccc gctcggcgtg cgccggctgg aggacggtcg gctggagctg
     1081 gattttcccg cccgcccgct gctcgccacc gacgccgggc tcgccgaagc cgtcgccgcc
     1141 gcgctcggcg cgcagccggt cgaggtgcgc agctccgaca ccaacctgtt cgccgtcttc
     1201 gcctgcgaag ccgacatccg tgcgctgcgc ccggacttcg cggccctcgc ccgtctgccg
     1261 aagcagggcg tcatcgtcac cgcccccggc gatcatgccg acttcgtcag ccgctatttc
     1321 gccccggccg ccggcatcga cgaagacccg gtcaccggct ccgcccactg cgagctgacc
     1381 ccgtactggg ccgcacggct gggccgggat cgcctgcacg cgcagcaggt ctcgaagcgc
     1441 cacggcgaac tgcaggtatg tctcgatggc gcgcgcgtgc gcatcgccgg ccatgccgtg
     1501 ccgtatctcg aagggcggat cacgctgccc ggagactgag gcggttcaac gctgccggcg
     1561 cggtttcggg tccgttgcgg agccgctgcc ggccggcacc gatccgcagg ctttccggac
     1621 cccgaaacgc caccggccgc gcagggcgcg gccggtgggt tgtcctgtcc ggctttcgca
     1681 acgtcgccgg tgtcgtcgcg aaggccgggc cggagcagga ttactggact tcgcggatat
     1741 acagcccgtg cacgccaacc gcttccaggt gccgcttgac cgaaaccgcg ttgtacaggg
     1801 tttcgtattc ggcggcctgc ggctgatccg gggccgtgaa ctgcgtgctg aacggctgcg
     1861 aggaagtcgg acgcgaggct tccctggaaa cgacctgacc ctgatgttcg gtgctgaaaa
     1921 tccggtagct catct
//
LOCUS       DAOQJH010000600         1930 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_49338_length_1930_cov_4.119467, whole genome shotgun sequence.
ACCESSION   DAOQJH010000600 DAOQJH010000000
VERSION     DAOQJH010000600.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1930)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1930)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1930
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49338_length_1930_cov_4.119467"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..842
                     /gene="csb2"
                     /locus_tag="PLQ18_12265"
     CDS             <1..842
                     /gene="csb2"
                     /locus_tag="PLQ18_12265"
                     /inference="COORDINATES: protein motif:HMM:TIGR02165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type I-U CRISPR-associated protein Csb2"
                     /protein_id="HNE02471.1"
                     /translation="GWNRPPAAIEIPYRRRPARPSAPRHDRRPGPAIDTVRYALYGRP
                     LPRIEDAIRVGEWLRSTVLRACHPPVPALISGHDLPPGNPHAHAFWLAESAGGDGRID
                     HVLIHVPDGIPAGVFGVLADPGELREPARRRPAGDGDADDTDTRAWQLLPEWFGRAAT
                     PPDGDAGRLLGKATVWRSTTPYLHPWHVKRGFGAAEQIRRECRERGLAEPEVRELEHI
                     EVAGRRLRPLDFHRFRRKPVAIQPDARGHALELRFPEPVSGPLALGFGCHFGLGTFGR
                     GEG"
     gene            952..1134
                     /locus_tag="PLQ18_12270"
     CDS             952..1134
                     /locus_tag="PLQ18_12270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019486655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HNE02472.1"
                     /translation="MKYSEFVRWLKSHGAEISQGKGRHYLKATYNGRTVPVPYHGAKE
                     IPEGVRKSILKQLGLS"
     gene            1183..1608
                     /locus_tag="PLQ18_12275"
     CDS             1183..1608
                     /locus_tag="PLQ18_12275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005000510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HNE02473.1"
                     /translation="MLAYALNVVPDTVGFMSTCRDLPEFVGVGDSIEDALRLAVEGLE
                     TTIGIYIDERRPVPMPSAPEPGEHAVYVPALTAAKALLSNAMIETGWRKADLVRALGV
                     HPPQVDRLLDVNHHSKLDQIEAALLALGRRLVVEVQRAA"
BASE COUNT          302 a          726 c          619 g          283 t
ORIGIN      
        1 ccggctggaa ccgcccgccc gcagccatcg agattccgta tcgccgccgc ccggcacgac
       61 cgtccgcacc ccggcacgac cgccgccccg ggccggcgat cgacacggtg cgctacgcgc
      121 tgtacggccg gccgctgccg cgcatcgagg atgcgatccg cgtcggcgag tggctgcgca
      181 gcaccgtcct gcgcgcctgc cacccgccgg tgcccgcgct gatcagcggc cacgacctgc
      241 cgcccggcaa tccccacgcc catgccttct ggctcgccga gagcgccggc ggcgacggcc
      301 gcatcgacca tgtgctgatc cacgtgccgg acggcatccc ggccggggtg ttcggcgtgc
      361 tggccgatcc gggcgaactg cgcgaaccgg cccggcgccg gcccgccgga gacggcgatg
      421 ccgacgacac cgacacccgc gcctggcaac tgctgcccga atggttcggc cgcgccgcca
      481 cgccaccgga cggggacgcc gggcggctgc tcggcaaggc cacggtctgg cgcagcacga
      541 cgccgtacct gcacccctgg catgtgaaac gcggcttcgg ggccgccgag cagatacggc
      601 gcgaatgccg cgagcgcggg ctggcggaac cggaagtccg ggagctggag cacatcgaag
      661 tcgccggccg ccggctgcgc ccgctcgatt tccaccgctt ccgccgcaag ccggtcgcca
      721 tccagcccga tgcccgcggt cacgcgctcg aactgcgctt tccggaaccg gtcagcggcc
      781 cgctggcgct cggcttcggc tgccatttcg gactcgggac gttcggacgc ggcgagggct
      841 gataccggtc acccggggac agacatctga cccgcccgtc atcctccggc gcctgtttac
      901 tcgacatcac ttctgttgta tttcgccttt agcaaaccaa gaggccggag gatgaagtac
      961 agcgagttcg tcagatggct gaagagtcac ggcgccgaga tcagtcaggg caagggtcgt
     1021 cactacctga aggcgacgta caacggcagg acggtgccgg tgccatacca cggggcgaag
     1081 gaaatccctg aaggcgtccg gaaatcgatt ctgaaacagc tggggctcag ttgatgcccg
     1141 gtttctcgct ggctttgccc ccggccgcaa caggagacca ccatgctggc ctatgccctg
     1201 aacgtcgtcc ccgacaccgt cgggttcatg agcacctgcc gtgacctgcc ggaattcgtc
     1261 ggggtcggcg acagcatcga ggatgccctc cggcttgccg tcgaggggct ggaaaccacg
     1321 atcggcatct acatcgacga acgccgcccg gtgccgatgc cttctgcgcc cgagcccggc
     1381 gaacacgcgg tctacgtgcc ggcgctgacg gccgcgaagg cgctgctcag caacgcgatg
     1441 atcgaaaccg gctggcgcaa ggccgatctc gtgcgggcac tcggtgtgca cccgccgcag
     1501 gtcgatcgtc tgctcgacgt gaaccaccac tcaaagctcg accagatcga agcggccctg
     1561 ctcgcactcg gccgtcggct ggtcgtcgag gtgcagcggg cagcctgagc gcagcccgcc
     1621 ggcacttgaa tcccggcgga cgcacccgac ccgtgctgcg tcctcagctt ccgtcgcgac
     1681 gccgcccgac gtattcccgc agcgcctgat tgatggcgct ccggtaacgg tcgctgcggg
     1741 ccttgaacca gtcgatcggg tcggcatcga gccggccagc acactctttc ctatccagcc
     1801 catcagggca tcaggctcag cttttgaccc tcccccctgc gacgctccaa cgctccggcc
     1861 cgacgcgacc ggctagctag tcgctgcccc cgcgccccgc cccgccctgc acgctttgcc
     1921 catccgcaca
//
LOCUS       DAOQJH010000601         1930 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_49339_length_1930_cov_4.042667, whole genome shotgun sequence.
ACCESSION   DAOQJH010000601 DAOQJH010000000
VERSION     DAOQJH010000601.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1930)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1930)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1930
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49339_length_1930_cov_4.042667"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..654)
                     /locus_tag="PLQ18_12280"
     CDS             complement(<1..654)
                     /locus_tag="PLQ18_12280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008954260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNE02474.1"
                     /translation="MARFRLPAVAGTCLFPLLLLSAPLHAGPAVALGYAPKYGPGFTH
                     FEYVNPDAPKGGTLTLDAIGGFDRLNPWLLKGTPAAGVADLMCDTLVEQSRDEPFSVY
                     GLLADDIALAPDRRSVRFHLRPEARFADGSPVRAADVKASFDTLTGKFAHPQYRLYWA
                     DVQAAVVEDAQSVRFEFARENPELHLIIGQMPVFAAKWIEGRQFDEIVREAPLCSGPY
                     "
     gene            760..1575
                     /locus_tag="PLQ18_12285"
     CDS             760..1575
                     /locus_tag="PLQ18_12285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase"
                     /protein_id="HNE02475.1"
                     /translation="MGFLAGKRALIVGLASNRSIAYGIARAMHREGAELAFTYQNDKL
                     KPRVEKMAAEFGSTLTWPCDVSNDAEIDAVAEALAGAWPEGYDIVVHSVAFAPGEALD
                     GDFLDGISRENFRIAHDVSSYSFAALAKATRPKLRAGGALLTLSYLGAMRSIPNYNVM
                     GLAKASLEANVRYLAATLSPAQLDADGKFVAHGVRVNAISAGPIRTLAASGIAGFRKM
                     LEAFEAAAPTRQCVTIDEVGNVAAFLCSDLASGMTGEIVYVDGGFHNVAMANL"
     gene            complement(1705..>1930)
                     /locus_tag="PLQ18_12290"
     CDS             complement(1705..>1930)
                     /locus_tag="PLQ18_12290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02476.1"
                     /translation="PDMIERLARAQLASGDRAVIVLKSVQPGTEADVAEADRQALTQR
                     AALQSGERDFRGLLDALRAAAKIENRADTL"
BASE COUNT          306 a          700 c          645 g          279 t
ORIGIN      
        1 atacggcccg ctgcagagcg gggcttcacg cacgatctcg tcgaactggc gcccctcgat
       61 ccacttcgcc gcgaacaccg gcatctggcc gatgatcagg tgcagctcgg ggttctcgcg
      121 cgcgaactcg aagcgcacgc tctgcgcatc ctcgacgacg gcggcctgca cgtcggccca
      181 gtacagccgg tactgcggat gcgcgaattt gccggtcagc gtgtcgaagc tcgccttcac
      241 gtcggccgcg cgcaccggcg agccatcggc gaagcgcgcc tcgggccgca ggtggaagcg
      301 caccgagcgc ctgtccggcg cgagcgcgat gtcatcggcg agcaggccgt agacgctgaa
      361 gggttcgtcg cgcgactgct cgaccagcgt gtcgcacatc aggtccgcca cgccggccgc
      421 cggcgtgccc ttcagcagcc acgggttcag gcggtcgaag ccgccgatcg catcgagcgt
      481 cagcgtgccg cccttgggcg catcgggatt cacgtattcg aaatgcgtga agccggggcc
      541 gtacttcggg gcatagccga gcgcgaccgc aggcccggcg tgaagcggcg cggacagcag
      601 cagcagcggg aacaggcagg tgccggcgac ggccggcagg cggaatcggg ccatcggcga
      661 tcctttgagg gcggaaaccc gcaggttatg ctttcaggat tgaccgggag ccgggaacgg
      721 ccggctttcg acgatagcaa atgaccacag gagtcctcca tgggttttct cgccggcaag
      781 cgcgccctga tcgtcggtct cgccagcaac cgctcgatcg cctacggcat cgcccgggcc
      841 atgcaccgtg aaggcgccga actcgcgttc acgtaccaga acgacaagct caagccccgc
      901 gtcgagaaga tggccgccga gttcggcagc acgctgacct ggccctgcga tgtcagcaac
      961 gatgccgaga tcgacgccgt cgccgaagcc ctcgccggcg cctggcccga gggctacgac
     1021 atcgtcgtgc attcggtggc gttcgcgccc ggcgaggcgc tcgatggcga cttcctcgac
     1081 ggcatctcgc gcgagaactt ccgcatcgcc cacgacgtga gttcctacag cttcgcggcc
     1141 ctcgccaagg ccacgcgccc gaagctgcgt gccggcggcg cgctgctgac gctcagctac
     1201 ctcggcgcga tgcgcagcat cccgaactac aacgtcatgg gcctcgccaa ggccagcctc
     1261 gaagccaacg tccgctatct ggccgcaacg ctgagtccgg cgcagctcga cgccgacggc
     1321 aagttcgtcg cgcacggcgt gcgcgtcaac gccatctcgg ccggtccgat ccgcacgctt
     1381 gcggccagcg gcatcgccgg cttccgcaag atgctcgaag ccttcgaggc cgccgcgccg
     1441 acgcgccagt gcgtgaccat cgatgaagtc ggcaacgtcg cggccttcct gtgctcggac
     1501 ctggcctccg gcatgaccgg cgagatcgtc tacgtcgacg gcggcttcca caacgtcgcg
     1561 atggcgaacc tgtaagcgcc cccgctcctc aaggcggaca cgacgcgggc ccgctgccgg
     1621 atcgacggca gcgggcccgc gcatgtccgg gccggtcctg atccggaccg acccggtatc
     1681 cggacaggcc ggtcgcccgc gcaatcagag cgtgtcggcg cgattctcga tcttcgccgc
     1741 cgcacgcagc gcatcgagca gaccgcggaa gtcgcgctcg ccggactgca gcgccgcccg
     1801 ctgcgtgagc gcctgccggt cggcttccgc cacgtcggcc tcggtcccgg gctgcacgga
     1861 cttcagcacg atcaccgccc ggtcgccgct ggccagctgc gcgcgggcca ggcgttcgat
     1921 catgtcgggg
//
LOCUS       DAOQJH010000602         1925 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_49602_length_1925_cov_4.136898, whole genome shotgun sequence.
ACCESSION   DAOQJH010000602 DAOQJH010000000
VERSION     DAOQJH010000602.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1925)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1925)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1925
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49602_length_1925_cov_4.136898"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..302
                     /locus_tag="PLQ18_12295"
     CDS             <1..302
                     /locus_tag="PLQ18_12295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015634334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HNE02477.1"
                     /translation="HTAMAGCVGIAGSTTVGAHCTLAGGVGLAGHLTLGDHVHVTGMS
                     LVTKSLTEPGVYASGLAVEPARLWNKVSARLRRLDELFRRLAALEKRVGDDAGHP"
     gene            324..764
                     /gene="fabZ"
                     /locus_tag="PLQ18_12300"
     CDS             324..764
                     /gene="fabZ"
                     /locus_tag="PLQ18_12300"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011717683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HNE02478.1"
                     /translation="MDITQVLRYLPHRYPFLLIDRVLELEPGERLLGLKNVSFNEPFF
                     QGHFPQQPVMPGVLILEALAQATGILAFKTSGQLPEDDSTYYFVGIDNARFKQPVCPG
                     DRLLLDVRLLKTRRGIWVFSAEARVDDKVVCSAELMSTKREHGQ"
     gene            761..1531
                     /gene="lpxA"
                     /locus_tag="PLQ18_12305"
     CDS             761..1531
                     /gene="lpxA"
                     /locus_tag="PLQ18_12305"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HNE02479.1"
                     /translation="MIDPRAIVDPGARIAEGVQIGPFSIIGAGVEIETGTVIGPHVVI
                     KGPTRIGRDNRIYQFASIGDDPQDKKYAGEPTRLEIGDGNTIREYVTINRGTVQDAGV
                     TRVGHDNWIMAYVHIAHDCVVGDHTIFANNASLAGHVHVGDWAILGGYTLVHQFCRIG
                     AHSFCGFATGVQRDVPPYVTVAGYRAEPYGINSEGLRRRGFEAETIAVIKRAYKLLYM
                     SDLKLAEAVERMRELVATHPPVALLADFVADSPRGIVR"
     gene            1537..>1925
                     /locus_tag="PLQ18_12310"
     CDS             1537..>1925
                     /locus_tag="PLQ18_12310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019019612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HNE02480.1"
                     /translation="MRIAVVAGELSGDALGGALLPALQQRFPQARFEGIGGPRMQAAG
                     LESLFPLEKLSVMGLVEVLKHLPELLSIRRALVRRWLAEPPALYLGIDAPDFTLPLAK
                     RLRAHGIRTAHYVSPSVWAWRQGRVRG"
BASE COUNT          294 a          680 c          638 g          313 t
ORIGIN      
        1 cgcacacggc gatggccggc tgcgtcggca ttgccggcag cacgacggtc ggcgcgcact
       61 gcacgctcgc cggaggcgtc ggtctggccg ggcacctgac gctcggcgac cacgtccacg
      121 tcaccggcat gtcgctggtc accaaatccc tgaccgagcc cggcgtctat gcgtccggac
      181 tcgcggtcga gccggcccgg ctgtggaaca aggtttccgc gcgcctgcgc cggctcgacg
      241 aactgttccg ccggctcgcc gcgctcgaaa aacgtgtcgg cgacgatgcc ggtcacccct
      301 agaggacccc gcccttggaa tcgatggata tcacgcaggt gctgcgttat ctgccgcacc
      361 gctatccgtt tctgctgatc gaccgcgtgc tcgaactcga gcccggcgaa cgcctgctcg
      421 gcctgaagaa cgtctcgttc aacgaaccct tcttccaggg ccatttcccg cagcagccgg
      481 tgatgccggg cgtgctgatc ctggaagcac tcgcgcaggc caccggcatc ctcgccttca
      541 agacctccgg ccagctgccc gaggacgatt cgacctatta cttcgtcggc atcgacaatg
      601 cccgcttcaa gcagccggtc tgtccgggcg accggctgct gctcgacgtg cgcctgctca
      661 agacccgccg cggcatctgg gtgttctcgg ccgaggcccg cgtcgatgac aaggtggtgt
      721 gctcggccga actgatgtcc acgaagcggg agcacggcca gtgatcgacc cgcgcgccat
      781 cgtcgatccg ggggcccgca tcgccgaagg cgtgcagatc gggccgttca gcatcatcgg
      841 tgccggtgtc gagatcgaga ccggcaccgt gatcggcccg cacgtcgtga tcaagggacc
      901 gacacgcatc gggcgcgaca accgcatcta ccagttcgcg tcgatcggcg acgatccgca
      961 ggacaagaaa tacgccggtg agccgacgcg gctggaaatc ggcgacggca acacgatccg
     1021 cgaatacgtg acgatcaacc gcggcacggt gcaggacgcc ggggtcacgc gcgtcggcca
     1081 tgacaactgg atcatggcct acgtgcacat cgcgcacgac tgcgtggtcg gggaccacac
     1141 gatcttcgcg aacaacgcct cgctcgccgg acacgtgcac gtcggcgact gggcgatcct
     1201 cggcggctac acgctggtgc accagttctg ccgcatcggt gcgcacagct tctgcggctt
     1261 cgcgaccggc gtgcagcgcg acgtgccgcc gtacgtgacc gtcgccggct atcgcgccga
     1321 gccttacggc atcaacagcg agggcctgcg ccgccgcggt ttcgaggccg agaccatcgc
     1381 cgtgatcaag cgcgcctaca agctgctgta catgtcggat ctgaagctcg ccgaggccgt
     1441 cgagcgcatg cgcgagctgg tcgccacgca tccgccggtg gcgctgctcg ccgatttcgt
     1501 cgccgattcg ccgcgcggca tcgtgcgctg acgcgcatgc gcatcgcagt cgtcgccggg
     1561 gagctctcgg gcgatgcgct tggcggcgcg ctgctgccgg cgttgcagca gcgttttccg
     1621 caggcgcgct tcgagggcat cggcgggccg cgcatgcagg cggccggtct cgaatcgctg
     1681 ttcccgctcg aaaaactgtc ggtgatgggg ctggtcgagg tgctgaagca cctgccggag
     1741 ctgctgtcga tccgccgcgc gctcgtgcgc cgctggctcg ccgagccgcc cgccctgtac
     1801 ctcggcatcg acgcacccga tttcacgctg ccgctcgcga agcgcctgcg tgcgcacggc
     1861 atccgcactg cgcattacgt cagcccctcg gtctgggcct ggcggcaggg gcgggtgcgc
     1921 ggcat
//
LOCUS       DAOQJH010000603         1923 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_49696_length_1923_cov_4.550321, whole genome shotgun sequence.
ACCESSION   DAOQJH010000603 DAOQJH010000000
VERSION     DAOQJH010000603.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1923)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1923)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1923
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49696_length_1923_cov_4.550321"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(260..1048)
                     /locus_tag="PLQ18_12315"
     CDS             complement(260..1048)
                     /locus_tag="PLQ18_12315"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE02481.1"
                     /translation="MKIQDLTVLLVEPSATQAKIVRQQLQALGVTRIETVTSGAAALP
                     VVQARDLHLVVSRLYLPDMSGADLSKTINALDRPFRPAFMLISSETNEAYLEPLRQAG
                     AAAIVRKPFDASFMQEALHAVLDHLEPGTLHLGERDIEEIRVLLADDSTAVRRFLVQL
                     LRGLGIDRIDEADDGATAMPFIDQHYYDLLITDLHMPLLNGIELTRYVRCESQQRSLP
                     IVMITSEADGGRLDMARAAGVSAFLHKPFEAKALKAHIERLLAG"
     gene            complement(1137..>1923)
                     /locus_tag="PLQ18_12320"
     CDS             complement(1137..>1923)
                     /locus_tag="PLQ18_12320"
                     /EC_number="1.17.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019625422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HNE02482.1"
                     /translation="NWGSLDQELLARLMDENHRRPEPWDAATVMHEALVASALESAGA
                     AEALGLAHERIVLSAKVSGVQDLIAVYRKLAARCDYPLHLGLTEAGMGSKGIVASTAG
                     LAVLLQEGIGDTIRISLTPEPGAAREQEVIVAQEILQCMGLRSFTPMVISCPGCGRTT
                     SDYFQRLAQQIQSYLRAQMPLWRAEHPGVEAMTVAVMGCVVNGPGESKMANIGISLPG
                     ADEDPVAPVYVDGKKTVTLKGDTIAADFQAIVDDYVKSRFTRT"
BASE COUNT          277 a          652 c          678 g          316 t
ORIGIN      
        1 ctccgtcccg gcatgcacgc cgcggcgttc gagctgttcg cccaggcgtt gcagcagggg
       61 atcgtcatcc ggcgggcggc ggtcgttcat cggcagggtt ctcccggagt cctgtaagac
      121 gcagcttcat gcaccgcgcc ggtgagccgc cgggttcatg cccggccagc atctgcatgc
      181 cggcgattgt gccccgaacc atcgcccggc aaaccctacg atcggaatag gtactccctg
      241 cggccacgca tcttcacagt cagccggcca gcagccgctc gatgtgcgcc ttcagcgcct
      301 tggcctcgaa gggcttgtgc aggaaggccg agaccccggc ggcacgcgcc atgtccagcc
      361 gcccgccgtc ggcctcgctg gtgatcatca cgatcggcag cgagcgctgc tggctctcgc
      421 agcgcacgta gcgcgtcagc tcgatgccgt tcagcagcgg catgtgcagg tcggtgatca
      481 gcaggtcgta gtagtgctgg tcgatgaacg gcatcgccgt cgcgccgtcg tcggcctcgt
      541 cgatgcgatc gatgccgagg ccgcgcagca actgcacgag gaagcggcgc acggccgtcg
      601 aatcgtcggc gagcagcacg cggatctcct cgatgtcgcg ctcgccgagg tgcagcgtgc
      661 ccggttcgag gtgatcgagc accgcgtgca gcgcctcctg catgaagctc gcgtcgaagg
      721 gcttgcgcac gatcgccgcg gcgccggcct ggcgcagcgg ttcgagatag gcctcgttgg
      781 tctcgctgga gatcagcatg aaggccgggc ggaacggccg gtcgagggcg ttgatggtct
      841 tggagagatc ggctccggac atgtcgggca ggtacaggcg gctgacgacc agatgcaggt
      901 cgcgcgcctg cacgaccggc agcgccgcgg cgccgctcgt gaccgtctcg atgcgcgtga
      961 caccgagggc ctgcagctgc tggcgcacga tctttgcctg ggtggccgag ggctcgacca
     1021 gcagcaccgt gagatcctga atcttcatgg cccgacgctc tcctctggtg taccggcccg
     1081 tctgtgcggg catggggaat ccagtctagg cgcgctgccc cgggcctgtg tccggcctac
     1141 gtccgggtga aacgcgactt cacgtagtca tcgacgatgg cctggaaatc ggccgcaatc
     1201 gtgtcgccct ttagggtcac ggtctttttt ccgtcgacgt agaccggcgc gaccgggtcc
     1261 tcgtcggcgc cgggcagcga gatgccgatg ttggccatct tgctttcgcc ggggccgttg
     1321 acgacgcagc ccatcaccgc gaccgtcatg gcctcgacgc ccgggtgctc ggcacgccag
     1381 agcggcatct gcgcgcgcag gtaggactgg atctgctgcg cgaggcgctg gaagtagtcc
     1441 gaggtcgtgc gcccgcagcc ggggcaggag atcaccatcg gcgtgaacga gcgcaggccc
     1501 atgcactgca ggatctcctg cgcgacgatg acctcctgct cgcgcgccgc gccgggctcc
     1561 ggcgtcagcg agatgcggat cgtgtcgccg atgccttcct gcagcagcac ggcgaggccg
     1621 gcggtcgagg cgacgatgcc cttcgagccc atgccggcct cggtcaggcc gaggtgcagc
     1681 gggtagtcgc agcgcgcggc gagcttgcgg tagacggcga tcaggtcctg cacgcccgag
     1741 accttggccg acagcacgat gcgctcgtgc gcgagcccca gcgcctcggc ggccccggcg
     1801 ctttcgagcg cggaggcgac cagcgcctcg tgcatgacgg tcgcggcgtc ccagggctcg
     1861 ggccggcgat ggttctcgtc catcagccgc gcgagcagct cctggtccag gctgccccag
     1921 ttg
//
LOCUS       DAOQJH010000604         1922 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_49763_length_1922_cov_3.713444, whole genome shotgun sequence.
ACCESSION   DAOQJH010000604 DAOQJH010000000
VERSION     DAOQJH010000604.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1922)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1922)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1922
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49763_length_1922_cov_3.713444"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..615)
                     /locus_tag="PLQ18_12325"
     CDS             complement(<1..615)
                     /locus_tag="PLQ18_12325"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HNE02483.1"
                     /translation="MTRFIFVTGGVVSSLGKGIAAASLAAILEARGLQVTMIKLDPYI
                     NVDPGTMSPFQHGEVYVTEDGAETDLDLGHYERFVRMTATRRNNFTTGRIYENVIRKE
                     RRGDYLGGTVQVIPHITDEIKRCVRLGAGNADIVMVEIGGTVGDIESLPFLEAIRQLG
                     VELGRERALFMHLTLLPYIATAGELKTKPTQHSVKELRSIGIQPD"
     gene            complement(612..>1922)
                     /gene="tilS"
                     /locus_tag="PLQ18_12330"
     CDS             complement(612..>1922)
                     /gene="tilS"
                     /locus_tag="PLQ18_12330"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HNE02484.1"
                     /translation="VLAHLRPAGLQLDALHVDHGWRPESADWARHCIARATALGVGCR
                     VLRIEARAAPGESPEAAARTARYAALGARLDAGNALLTAQHADDQAETVLLMLLRGAG
                     PKGLAAMPEDAALGSGRLWRPLLGVSRAALQAYATHYRLDWLDDPANTDTDYDRVFLR
                     ERLMPVLAGRFPAAARTLARSARLSAEAQAAVETLAAQDLDGLTGGGAGPAGTGGTLA
                     VAGLQALSPARARAALRLWIERLGLPLPPAARLDTVLRDLLPARRDAEPCVCWPGAEM
                     HRFRERLHVFAPLPALPDALCLDWPPEGGEISIAGLGTLVWQPASGAGLDAARLAGRR
                     VTLRLRQGGERFRPAGRRHAQELKKLLQAAGIAPWLRARLPLLYIDEELVAVAGLGIA
                     EGWLAGPDAPGRVPAWQTQAPSSTMPRFVAAPLGAREAGLEHFP"
BASE COUNT          261 a          695 c          714 g          252 t
ORIGIN      
        1 gtcgggctgg atgccgatcg agcgcagttc cttgaccgag tgctgcgtcg gcttggtctt
       61 cagctccccg gccgtggcga tgtacggcag cagcgtcagg tgcatgaaca gcgcacgctc
      121 gcggccgagt tcgacgccga gctggcggat cgcttcgagg aagggcagcg actcgatgtc
      181 gccgaccgtg ccgccgatct cgaccatgac gatgtcggcg ttgccggcac cgaggcgcac
      241 gcagcgcttg atctcgtcgg tgatgtgcgg gatcacctgc acggtgccgc cgaggtagtc
      301 gccgcggcgt tccttgcgga tgacgttctc gtagatgcgg ccggtcgtga agttgttgcg
      361 ccgcgtggcg gtcatgcgca cgaagcgctc gtagtggccg agatcgagat cggtctccgc
      421 gccgtcctcg gtcacgtaca cctcgccgtg ctggaacggg ctcatcgtgc ccggatcgac
      481 gttgatgtac ggatcgagct tgatcatcgt gacctgcagc ccgcgcgctt cgaggatcgc
      541 ggcaagcgag gccgcggcga tgcccttgcc cagcgaggag acgacaccgc cggtgacgaa
      601 gatgaatcgg gtcatgggaa gtgttccagg cccgcttcgc gggcaccgag aggggcggca
      661 acgaagcgcg gcatggtact ggatggcgcc tgcgtttgcc acgcgggcac ccgacccggt
      721 gcatcgggtc cggccagcca gccctcggcg atgcccaggc cggcgaccgc gaccagttcc
      781 tcgtcgatat aaaggagggg cagccgggcg cgcagccacg gtgcgatgcc ggcggcctgc
      841 agcagcttct tgagctcctg tgcatgccgg cggcccgcgg gccggaagcg ctcgccgccc
      901 tggcgcagcc gcagcgtcac gcgccggccg gcgagccgcg cggcatcgag gccggccccg
      961 cttgccggct gccagaccag cgtgccgagg ccggcgatgc tgatttcccc gccctccggc
     1021 ggccagtcga gacaaagcgc atccggcagc gccggcagcg gcgcgaacac gtgcagccgc
     1081 tcgcggaagc ggtgcatctc ggcgcccggc cagcacacgc agggctcggc atcgcggcgc
     1141 gccggcagca ggtcacgcag cacggtgtcg aggcgtgccg ccggcggcag cggcagcccg
     1201 agccgctcga tccagagccg cagcgccgcc cgcgcccgtg ccggcgacag cgcctgcagg
     1261 ccggcgaccg ccagcgtgcc gccggtcccg gcaggtccgg cgccgccgcc ggtcaggcca
     1321 tcgagatcct gcgcggccag cgtctcgacc gcggcctgcg cctcggcgct gagccgggcg
     1381 ctgcgcgcca gcgtgcgggc cgcggccggg aagcgcccgg cgagcaccgg catcagccgc
     1441 tcccgcagga acacgcggtc gtagtccgta tcggtgttgg ccgggtcgtc gagccagtcg
     1501 aggcggtaat gcgtcgcgta ggcctgcagc gcggcccgcg acactccgag cagcggccgc
     1561 cacagccgcc cgctcccgag ggctgcgtct tccggcatcg ccgcgagtcc cttcgggccg
     1621 gccccgcgca gcagcatcag cagcacggtt tcggcctggt catcggcatg ctgggcggtg
     1681 agcagcgcat tgccggcatc gaggcgcgcg ccgagcgccg cgtagcgcgc cgtgcgcgcc
     1741 gcagcctcgg gggattcgcc gggcgcggca cgggcctcga tgcgcagcac ccggcagccg
     1801 acaccgagcg ccgtcgcgcg cgcgatgcag tggcgcgccc agtcggcgga ctccggccgc
     1861 cagccgtgat cgacgtgcag cgcatcgagc tgcagcccgg ccgggcgcag gtgcgccagc
     1921 ac
//
LOCUS       DAOQJH010000605         1919 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_49901_length_1919_cov_3.061695, whole genome shotgun sequence.
ACCESSION   DAOQJH010000605 DAOQJH010000000
VERSION     DAOQJH010000605.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1919)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1919)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1919
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49901_length_1919_cov_3.061695"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            33..953
                     /gene="cbiB"
                     /locus_tag="PLQ18_12335"
     CDS             33..953
                     /gene="cbiB"
                     /locus_tag="PLQ18_12335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013088708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016880 - acid-ammonia (or amide) ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-phosphate synthase CbiB"
                     /protein_id="HNE02485.1"
                     /translation="MPMSSVSATVLVPALAYLLDRLLGEPPLRWHPLVWFGRWAGALE
                     RRLYRDSIRRGGLVWMLAVLPPTLLAGGLGALPGFGPWLGAGLLMLALGARSLDEHAQ
                     AVAVPLAAGELPAARQQLARIVSRDTATLDETGIARAAVESVLENGNDAICGALFWYA
                     LAGPAGAVGYRLANTLDAMWGYRTARHERFGKVAARIDDALNWLPARLCAASYALAGD
                     ARTAWRCWRTQARHWDSPNAGPVMAAGAGALGLALGGPASYGGHLETRPALGAGRAPQ
                     AAGIGRACALVRRASGLWVLLAMLSSGLRG"
     gene            1135..>1919
                     /gene="cobD"
                     /locus_tag="PLQ18_12340"
     CDS             1135..>1919
                     /gene="cobD"
                     /locus_tag="PLQ18_12340"
                     /EC_number="4.1.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007194516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0048472 - threonine-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine-phosphate decarboxylase CobD"
                     /protein_id="HNE02486.1"
                     /translation="MLEHGGRLNAAAARYRIDRADWLDLSTGINPDGWPVPQLPAEVW
                     QRLPEDDDALPIAVEQHYGRPGLPVAGSQAALATLPRLRAACRVGVIGPTYGEHAHAW
                     QRAGHEVRTLTVGAVEAALGQLDVLVAVNPDNPTGRLLASSTLLGWHRRLAARGGWLL
                     VDEAYMDAEPSESLLAVREQPGLIVLRSLGKFWGLAGVRAGFVFAEADLRAALAGLLG
                     PWSVSGPARAVAVRALADTHWQDHARACLGHAARRFARLLTRHG"
BASE COUNT          200 a          732 c          721 g          266 t
ORIGIN      
        1 cactcgcgcg cccccagacc gcccgccccg gaatgccgat gtcttctgtc tccgccaccg
       61 tgctcgtgcc cgcgctcgcg tacctcctcg accgcctgct cggcgaaccg ccgctgcgct
      121 ggcacccgct ggtctggttc ggtcgctggg ccggtgcgct cgaacgccgg ctgtaccggg
      181 attcgatccg gcgcggcggg ctggtctgga tgctcgcggt gctgccgccg acgctgctgg
      241 ccggcgggct cggggcgctg ccgggcttcg ggccgtggct cggcgccggg ctgctgatgc
      301 tcgcgctcgg cgcgcgcagc ctcgacgagc acgcgcaggc cgtggccgtg ccgctcgccg
      361 ccggcgagct gccggcggcg cggcagcagc tcgcgcgcat cgtctcgcgc gatacggcca
      421 cgctcgatga aaccggcatc gcccgcgcgg ccgtcgaatc ggtgctggaa aacggcaacg
      481 acgcgatctg cggcgcgctg ttctggtacg cgctggccgg gccggccggc gcggtcggct
      541 accggctggc gaacacgctc gatgcgatgt ggggctaccg gacggcgcgc cacgagcgct
      601 tcggcaaggt cgcggcacgc atcgacgacg cgctgaactg gctgccggcg cggctgtgcg
      661 cggcgagcta tgccctcgcc ggcgatgcac gcacggcctg gcgctgctgg cgcacgcagg
      721 cccggcactg ggacagcccg aacgccggcc cggtcatggc ggccggggcc ggtgcgctcg
      781 gcctcgcgct cggcggtccg gcgagctacg gcggtcacct cgaaacgcgt ccggcgctcg
      841 gtgccgggcg ggcgccgcag gccgccggca tcggccgggc ctgcgcgctg gtgcggcgcg
      901 cgagcgggct gtgggtgctg ctcgcgatgc tgagctccgg cctgcgcggc tgagcgccgg
      961 cgcgccttta ccgcggccct gcgcggcagg ggtgtaacga atttttcccg ctccgacaaa
     1021 gctgtcttga tgttaagcaa ggcgcgtgcc ggctctggcg gtatcatgcg tcccccgacc
     1081 gcgccggagc gtccgcaccg tcgcgcccga cctgcggaga ccccgatctt gatgctgctc
     1141 gaacacggcg gccggctgaa tgctgctgcc gcgcgctacc ggattgaccg tgccgactgg
     1201 ctcgacctgt cgaccggcat caacccggac ggctggccgg tgccccaact gccggccgag
     1261 gtctggcagc gcctgccgga ggacgacgat gcgctgccca tcgccgtcga gcagcactac
     1321 ggccggccgg ggctgccggt cgccggttcg caggccgcgc tggcgacgct gccgcggctg
     1381 cgcgcagcct gccgggtcgg cgtgatcggc ccgacctacg gcgagcacgc gcacgcctgg
     1441 cagcgcgccg gccacgaggt gcgcacgctg acggtcggcg cggtcgaggc cgcgctcggg
     1501 cagctcgacg tgctcgtcgc cgtcaacccc gacaacccga ccggccggct gctcgcttcg
     1561 agcacgctgc tcggctggca ccggcgactg gccgcgcgcg gcggctggct gctggtcgat
     1621 gaggcctaca tggacgccga gccgagcgag agcctgctcg ccgtgcgtga gcagcccggc
     1681 ctgatcgtgc tgcgctcgct cggcaagttc tggggcctgg ccggcgtgcg cgccggcttc
     1741 gtgttcgccg aggccgacct gcgcgccgcc ctcgccgggc tgctgggccc gtggagcgtc
     1801 agcggtccgg cacgcgcggt tgccgtccgg gcgctcgccg acacgcactg gcaggaccac
     1861 gcccgcgcct gcctcggcca cgccgcccgg cgcttcgcgc gcctgctaac ccgccacgg
//
LOCUS       DAOQJH010000606         1913 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_50177_length_1913_cov_4.358450, whole genome shotgun sequence.
ACCESSION   DAOQJH010000606 DAOQJH010000000
VERSION     DAOQJH010000606.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1913
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50177_length_1913_cov_4.358450"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..517
                     /locus_tag="PLQ18_12345"
     CDS             <1..517
                     /locus_tag="PLQ18_12345"
                     /inference="COORDINATES: protein motif:HMM:NF013989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HNE02487.1"
                     /translation="TVSLDGFLIPPGSAREEIDGGRTPLPEPGDRLPAPEVIIVGRHT
                     AAAARRAAHWPWPQQTVIALLHSESPAERDARLCYSTETPSGLLARLQRDGITRAQLA
                     GGSTLISVFHAAGLIDEWLLRVAPVLLGGGRPLFGPGTHPSTLTLVHAWGYPDGAVRL
                     HYRRGDAGIPA"
     gene            775..>1913
                     /locus_tag="PLQ18_12350"
     CDS             775..>1913
                     /locus_tag="PLQ18_12350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNE02488.1"
                     /translation="MSSEAVRERCARAVRRWSVLVPFALPVVLLVLLAGREAAPLLLP
                     LLVLAARIELRAAGALAACGLALVLLADRLGPLLLIDAAWFAPLVQVLALIACLGLAV
                     YPSVPVIRRAGGDDEAGRERTRLGALLSSLDDLAFEFDADGRYLDVWTARPELLLMPR
                     EQLLQRNLHDVLGQAEGDRVLGILRAVLADGEPREVEVRIRLADGRERQMLGRCARIA
                     PSVATAAATVSCLVRDVTAQRQVEQALRDSEARLRTILETLPVGAVHHHVPSGTVTLN
                     HAAERLLHYARAEVQRVEAWFARLYGEQAETVLALYLADRERGLDQVRTLDVHCGDGL
                     TRRVQFAGCASGEHEVWVMTDVTAHERLQQRLQATQAAVDAAEDAL"
BASE COUNT          219 a          709 c          692 g          293 t
ORIGIN      
        1 caccgtcagc ctcgacggtt tcctgatccc gcccggcagc gcgcgggagg agatcgacgg
       61 cggccggacg ccgctgcccg agcccggcga ccgcctgccc gcgcccgaag tcatcatcgt
      121 cggccggcac acggccgccg cggcacgccg ggccgcgcac tggccgtggc cgcagcagac
      181 cgtcatcgcg ctgctgcaca gcgaatcccc ggccgagcgg gatgcgcggc tctgctacag
      241 caccgaaacc ccgtccgggc tgctggcgcg cctgcagcgc gacggcatca cgcgtgcgca
      301 gctcgccggc ggcagcacgc tgatctcggt cttccacgcc gccggcctga tcgacgaatg
      361 gctgctgcgc gtggcgccgg tgctgctcgg gggcggccgg ccgctgttcg ggccgggcac
      421 gcatccctcg acgctgacgc tggtgcatgc ctggggctat ccggatggcg ccgtgcgcct
      481 gcactaccgt cgcggtgacg ccggcattcc tgcgtgagtc cacgtaaaag ccggtttcga
      541 aatggattcc tgtctgctgc aagccggtgt cgccggttgc cggcgccaca cggcaagtcg
      601 atgattatcg gttaaatacc agcaatgccg cgccggggac ggcgctgcct gtccgccggt
      661 gctgcgttcc gtgcccgctt gaggcccggc acctgcgggt gcgaggctcg cgccccgtgc
      721 ccgccagccg tctccgccgg cgctgccgtc cccccgcctt ccggtatgtt gccgatgtcg
      781 tctgaagctg tccgcgaacg ctgcgcgcgc gccgttcgcc gctggtccgt cctcgttccg
      841 ttcgcgttgc cggtggtgct gctggtgctg ctcgccgggc gggaggctgc accgctgctg
      901 ctgccgctgc tggtccttgc tgcccgcatc gaactgcgcg ccgcgggcgc actcgccgcc
      961 tgcggcctgg cgctggtgct gctcgccgac cgcctcgggc cgctgctgct gatcgacgcc
     1021 gcctggttcg cgccgctggt gcaggtgctt gcgctgatcg cctgtctggg gctcgccgtg
     1081 tacccgtcgg tgccggtaat ccgccgcgcg ggtggggacg acgaggccgg ccgggagcgc
     1141 acccggctcg gcgcgctgct gtcctcgctc gatgatctgg ccttcgaatt cgatgccgac
     1201 ggccgctatc tggacgtctg gaccgcccgg cccgagctgc tgctgatgcc gcgcgagcag
     1261 ctgctgcagc gcaacctcca cgacgtgctc gggcaggccg agggcgaccg ggtgcttggc
     1321 atcctgcggg ccgtgctcgc cgacggcgag ccgcgcgagg tcgaggtccg catccggctc
     1381 gccgatggcc gcgagcggca gatgctcggg cgctgcgcac gcatcgcgcc gtccgtggcc
     1441 actgcggcgg cgacggtcag ctgcctcgtg cgcgacgtga ccgcgcagcg tcaggtcgag
     1501 caggcgctgc gcgacagcga ggcgcggctg cggaccatcc tcgaaaccct gccggtcggt
     1561 gccgtccacc atcacgtgcc gagcggcacc gtgacgctga accacgccgc cgaacggctg
     1621 ctgcactacg cgcgcgccga ggtgcagcgc gtcgaggcct ggttcgcgcg gctgtacggc
     1681 gagcaggccg aaaccgtgct ggcgctgtat ctggccgatc gcgagcgcgg gctcgatcag
     1741 gtgcgcacgc tcgacgtgca ctgcggcgac ggcctcacgc gccgcgtgca gttcgccggc
     1801 tgcgcgagcg gcgaacacga ggtctgggtg atgaccgacg tcacggcaca cgagcgcctg
     1861 cagcagcgcc tgcaggcgac gcaggccgcc gtcgatgccg ccgaggacgc gct
//
LOCUS       DAOQJH010000607         1910 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_50343_length_1910_cov_3.882480, whole genome shotgun sequence.
ACCESSION   DAOQJH010000607 DAOQJH010000000
VERSION     DAOQJH010000607.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1910)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1910)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1910
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50343_length_1910_cov_3.882480"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1697)
                     /locus_tag="PLQ18_12355"
     CDS             complement(<1..1697)
                     /locus_tag="PLQ18_12355"
                     /inference="COORDINATES: protein motif:HMM:NF016944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004352 - glutamate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019551 - glutamate catabolic process to
                     2-oxoglutarate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase"
                     /protein_id="HNE02489.1"
                     /translation="MSSSPADKKAELIERVAARLTERLPAGGAVAAQFARVYFARVAP
                     EDLINDDPEDLYGAVLSHWNLLRRTRTGEIGLRVYNPGYDEHGWRSTHTAIELVVPDM
                     PFLVDSVSMELNRHGFTVHRLIHPVLAIAYGEDGEPLSVAETGAGDGVQLAVMQFAID
                     RETDAARLETLRDDLARVLDDVRAAVEDWPRMLARLNEIADELDAAKLPVPVAEVEEA
                     RAFLDWAADNHFTLLGYSGYRLEELDGELVLHPEPGTGLGLRRGAAAHHDTRFNALPP
                     QHRRLALAPVLLVMTKANARSTVHRPVQLDYLGIKRFDEAGRVIGEWRFLGLYTSSAY
                     QTSPFGIPVLRAKVRAVIERAGLPAVSHSGKALKHILATYPRDELLQASVDELYPIAT
                     GILHLEERQHLRVFIRIDAYERYATALIYVPRERYNTDLRKRMQDVLIAALNGHGADF
                     WVEFGESVLARVLFTIRTTPGHIPAYSVEEIRARLRETMLSWEDGLRAALIEAHGEGG
                     GIALYNRYAGAFGAAYQEDFTPRAAVGDIDRLEAVVGGAPLGLHLYRPQESPEGWLR"
BASE COUNT          273 a          650 c          695 g          292 t
ORIGIN      
        1 aagcgcagcc agccctcggg cgattcctgc ggccggtaca ggtgcaggcc gagcggcgcg
       61 ccgccgacga cggcttcgag ccggtcgatg tcgccgaccg cggcgcgcgg cgtgaagtcc
      121 tcctggtacg cggccccgaa ggcgccggcg tagcggttgt acagcgcgat gccgccgccc
      181 tcgccgtgtg cctcgatcag cgccgcgcgc aggccgtcct cccaggacag catcgtctcg
      241 cgcaggcgcg cgcggatttc ctcgacgctg taggccggga tgtggcccgg cgtcgtgcgg
      301 atcgtgaaca gcacgcgcgc cagcaccgac tcgccgaact cgacccagaa gtcggcgccg
      361 tgaccgttca gcgccgcgat cagcacgtcc tgcatgcgct tgcgcagatc ggtgttgtag
      421 cgctcgcgcg gcacgtagat cagcgccgtc gcgtagcgct cgtaggcatc gatgcggatg
      481 aacacgcgca ggtgctggcg ttcttcgagg tgcaggatgc cggtggcgat cgggtacagc
      541 tcgtcgacgc tcgcctgcag cagctcgtcg cgcggatagg tggcgaggat gtgcttcagc
      601 gccttgccgg aatggctgac cgccggcagc ccggcgcgct cgatcaccgc ccgcaccttg
      661 gcgcgcagca ccggaatccc gaacggactg gtctggtagg cgctcgacgt atacaggccg
      721 aggaagcgcc attcgccgat gacccggccg gcttcgtcga agcgcttgat gccgaggtaa
      781 tcgagctgca ccggccggtg cacggtcgag cgggcgttgg ccttggtcat gaccagcagc
      841 accggtgcca gcgccaggcg ccggtgctgc ggcggcagcg cgttgaagcg cgtgtcgtga
      901 tgcgcggcgg ccccgcggcg caggccgagc ccggtgcccg gctccgggtg cagcaccagc
      961 tcgccgtcga gttcttcgag ccggtagccg ctgtagccga gcagcgtgaa gtggttgtcg
     1021 gccgcccagt cgaggaaggc gcgcgcctcc tcgacctcgg cgaccggcac cggcagcttc
     1081 gccgcatcga gctcatcggc gatttcattg aggcgcgcca gcatgcgcgg ccagtcctcg
     1141 accgcggcgc gcacgtcatc gagcacgcgc gccagatcgt cgcgcagcgt ttcgaggcgg
     1201 gccgcatcgg tctcgcggtc gatcgcgaac tgcatcaccg cgagctgcac gccatcgccg
     1261 gccccggtct cggccaccga cagcggctcg ccgtcctcgc cgtaggcgat ggccagcacc
     1321 gggtggatca gccggtgcac ggtgaagccg tggcggttga gttccatgct caccgaatcg
     1381 accaggaacg gcatgtccgg cacgaccagc tcgatcgccg tatgcgtcga gcgccagccg
     1441 tgctcgtcgt agcccgggtt gtagacgcgc aggccgatct cgccggtacg cgtgcgccgc
     1501 agcaggttcc agtgcgagag cacggcgccg tagaggtctt cggggtcgtc gttgatcagg
     1561 tcctcgggtg cgacgcgcgc gaagtacacg cgcgcgaact gcgcggcgac cgcaccgccg
     1621 gccggcaggc gctcggtgag ccgcgccgcg acgcgctcga tcagttcggc cttcttgtcc
     1681 gctgggctgc tgctcatggt ggggaacctc ggattgcgct tctcgggagg gcgcgcccgt
     1741 cggcgggcgt ctgcttggtg tcacctgcgc cggcgccggg ttcggggaac gcggacggcg
     1801 taccgggcag ggggtggtag cgggagtggc gggaagtcta gacggcgggg gaaggcaggg
     1861 caaacgcatc ttcccatcct atgaaaacgc catacatgac ctgcacttgc
//
LOCUS       DAOQJH010000608         1893 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_51214_length_1893_cov_3.671382, whole genome shotgun sequence.
ACCESSION   DAOQJH010000608 DAOQJH010000000
VERSION     DAOQJH010000608.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1893)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1893)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1893
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51214_length_1893_cov_3.671382"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1306)
                     /locus_tag="PLQ18_12360"
     CDS             complement(<1..1306)
                     /locus_tag="PLQ18_12360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02490.1"
                     /translation="MALPSMTFPERPLQVSPPGSGRIANLGTLLWTTLLLLASAYFLL
                     PPIADLRDDFTIADTARPVQGGRVEGRCRTSLGVLVRCTATLTAPGRGGPQLQREVDY
                     FFVDFHLGDYTATVVADPVRPALLSTDLALERLWNRVYTLATAIPLGLGLLFGVLRLA
                     GQRIGERRSAVDALSGHVLHPVLLRLDSYLPGRWTVTPLPPTRDRRSRTWPVPRAARP
                     IVMDPERRLVLGVAAGSEELAMPLDTGLSWIGLERGERLRLREQLGPDRLGGWLPNLG
                     RAGLDDERAALSRRARRLGLSGGGLALLAALAAWVAWGGHDFSGDAIVLRRGDEGPAS
                     GEVRLNGIRHTELAVQTTRVDARAAHLEVWVPLTAPDWQAGQPVVWLIDDPEYGRGEP
                     RAATLVGSVAGPVPDRAREQLARLHVQLSPEARVLAARAWEPP"
     gene            complement(1473..>1893)
                     /locus_tag="PLQ18_12365"
     CDS             complement(1473..>1893)
                     /locus_tag="PLQ18_12365"
                     /inference="COORDINATES: protein motif:HMM:NF036497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HNE02491.1"
                     /translation="AALRNSIGLLRVGDEVRLGLLREGKPLSLTARLGEGRGGRVADS
                     SGGGGKALNPRLEGASFGNLPDGHPLSGEVDGVLVNAVEAGSPAARGGLRRGDVILGV
                     NREPVAGIEDFAQAVTGNRALLLQVLRGRSSLFLMLQ"
BASE COUNT          267 a          689 c          713 g          224 t
ORIGIN      
        1 acggcggctc ccaggcgcgg gcggcgagca cgcgcgcctc cgggctcagc tgcacgtgca
       61 gccgcgccag ctgctcgcgc gcgcgatccg gcaccggccc ggcgacgctg ccgacgaggg
      121 tcgcggcccg cggctcgccg cggccgtatt cgggatcgtc gatcagccag accaccggct
      181 gaccggcctg ccagtccggg gcggtcagcg gcacccagac ctccagatgc gcggcgcgcg
      241 catcgacgcg cgtggtctgc acggcgagtt cggtgtggcg gatgccgttc aggcgcacct
      301 cgccgctggc cgggccttcg tcgccgcgcc tcagcacgat ggcatcgccg ctgaaatcat
      361 gaccgcccca ggccacccag gccgccagcg cggcgagcag cgccagtccg cccccgctca
      421 gcccgagccg ccgggcccgg cgcgacaggg ccgcacgctc gtcatcgagg ccggcgcgcc
      481 cgaggttcgg cagccagcca ccgaggcgat cggggccgag ctgttcgcgc aggcgcaggc
      541 gttcgccgcg ctccagcccg atccacgaca ggccggtgtc gagcggcatc gccagttcct
      601 cgctgccggc agcgacaccg agcaccagcc gccgctccgg gtccatgacg atcggacgcg
      661 ccgcccgcgg caccggccag gtgcgcgagc ggcgatcgcg ggtcggcggc agcggcgtca
      721 cggtccagcg tccgggcagg tagctgtcca gacgcagcag caccgggtgc agcacgtggc
      781 cggacagcgc atcgacggcg ctgcgccgct cgccgatgcg ctgtccggcc aggcgcagca
      841 cgccgaacag caggccgagg ccgagcggga tggcggtcgc gagcgtgtag acgcgattcc
      901 acagccgttc gagcgccaga tcggtgctga gcagcgccgg gcgcaccgga tcggcgacga
      961 cagtcgccgt gtagtcgccg aggtggaaat cgacgaagaa gtagtcgacc tcgcgctgca
     1021 gttgcggccc gccgcgtccg ggtgccgtca gcgtcgccgt gcagcgcacc agcacgccga
     1081 gcgaggtccg gcagcggccc tcgaccctgc cgccctgcac cgggcgggcg gtgtcggcga
     1141 tcgtgaagtc gtcgcgcagg tcagcgatcg gcggcagcag gaaatacgcg ctggcgagca
     1201 gcagcagcgt ggtccacagc agcgtgccga ggttggcgat gcggccgctg ccgggcgggc
     1261 tgacctggag cggacgttcg gggaacgtca tggatggcag ggccatggac ggcgcctcct
     1321 cggattgggg aaagccgtcc agggcgtaac gggtgcgaca ttcagtcgct acggtgctga
     1381 gggcaggaag ataccgcggg ctggcggcga cacttgttgc agaacgtcac acggccggcc
     1441 cgggcaagcg cgggtggccg ggctttgcgc agctattgca gcatcaggaa cagcgagctg
     1501 cgtccgcgca gcacctgcag cagcagcgcg cggttgccgg tgaccgcctg cgcgaagtcc
     1561 tcgatgccgg cgaccggctc gcggttgacg ccgaggatca cgtcgccgcg gcgcaggccg
     1621 ccgcgtgcgg ccggcgaacc ggcctcgacg gcgttgacca gcacgccgtc gacctcgccg
     1681 gacagcggat ggccatcggg gaggttgccg aagctcgcgc cttcgaggcg cggattcagc
     1741 gccttgccgc ctccgccact gctgtcggcg acgcgcccgc cgcggccttc gccgagcctg
     1801 gcggtgaggc tgagcggctt gccctcgcgc agcaggccga ggcgcacctc gtcgccgacg
     1861 cgcagcaggc cgatgctgtt gcgcagcgcc gcc
//
LOCUS       DAOQJH010000609         1891 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_51334_length_1891_cov_3.713508, whole genome shotgun sequence.
ACCESSION   DAOQJH010000609 DAOQJH010000000
VERSION     DAOQJH010000609.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1891)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1891)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1891
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51334_length_1891_cov_3.713508"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            213..575
                     /locus_tag="PLQ18_12370"
     CDS             213..575
                     /locus_tag="PLQ18_12370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02492.1"
                     /translation="MSRFLLRWLIVLLLLPGPGLAAAPALAAGHGAPDRLALPVVDAG
                     PVLTARPGPGLHHDSGACPDTPASGQAHERCCDGCPAVPGTLVGLAAPAVPLASRLPY
                     RLPADPGHVTGPLRRPPR"
     gene            698..>1891
                     /locus_tag="PLQ18_12375"
     CDS             698..>1891
                     /locus_tag="PLQ18_12375"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNE02493.1"
                     /translation="MSRRLHRTGGALALLCLPFTLLAAPADADLPGATLAGLIEWAET
                     RNPELAVAGHELDAAEAMVGSADALPDPSFSIEWRDIDADRLTLDPSRVGSTKYTFAQ
                     TFPLWGKRGLQADIARAEAERARRQRSSVSVELRARLSAAHAERWQVRELLRLNAEQR
                     SLLRDLERIATARYADGLTAQSDAIRAQTERTQLEAERVRLEAERRRLGAQLNALLGR
                     RPDAPLADPVALSPLPAPAGFDGLQPRLLAANPRLAAQAAAVEAAGHTRELALAKRLP
                     DVTFGVSPVQRGSRLDGWEVMLAINIPLDQDARRADVQASAARLAAAEAGQVALALEL
                     QQSLAGTLAAYEAAGEQERLLRTRLRPQAEAALRSALAGYRSGRDDFTTLLDTLLLLT
                     IPSVGV"
BASE COUNT          208 a          738 c          680 g          265 t
ORIGIN      
        1 gttctcggtg caggcggtcg agcggctggc cgaggcgctg gcggaggtat gaggtccgtc
       61 cccttacgcg gcctgtactc tcggggccgc gctgtgccag cctttgcagc cgctgcgccg
      121 gcggcttgac cgccatcaac gacgaggcac cggcctgcgc gtcaggctgt tccggttttc
      181 cgctcctgcc cccccgaaac ttccgtacct ccgtgtcccg attcctgctg cgatggctga
      241 tcgtgctgct gctcctgccg gggccggggc tggcggctgc cccggcgctg gcggccgggc
      301 acggggcgcc cgaccggctg gcgctgccgg tggtcgatgc ggggccggtg ctcacggcgc
      361 gtccggggcc ggggcttcat catgattccg gtgcctgtcc ggacacgccc gcgtccggac
      421 aggcgcacga acgctgttgc gacggctgtc cggccgtgcc gggcacgctg gtcgggctgg
      481 ccgcgccggc ggtgccgctc gcctcgcgcc tgccgtaccg gctgccggcc gatcccgggc
      541 acgtgaccgg gccgctgcgc cggcctccgc gctgattcct cctcgccacg cctgcaccgt
      601 gccgtccgct gctgcggacg gtgcctgccg tgccgcgtcc gtcgccgccg aaggcgcgtg
      661 agaccggcac cccgcgccct gccaggagga catcccgatg tcccgtcgtt tgcaccgcac
      721 cggcggcgcg ctcgcgctgc tgtgcctgcc gttcacgctg ctcgcggccc ccgccgatgc
      781 cgatctgccc ggggccacgc tcgccggtct gatcgaatgg gctgaaaccc gcaatcccga
      841 actcgccgtg gccggccacg agctcgatgc cgccgaggcg atggtcggtt cggccgatgc
      901 gctgcccgac ccgagcttca gcatcgagtg gcgcgacatc gacgccgatc gcctgacgct
      961 cgatccgagc cgcgtcggtt cgacgaagta cacgttcgcg cagaccttcc cgctgtgggg
     1021 caagcgcggg ctgcaggccg acatcgcgcg cgccgaagcc gagcgcgcgc gccgccagcg
     1081 cagctcggtg tcggtcgagc tgcgcgcccg gctcagcgcc gcccacgccg aacgctggca
     1141 ggtgcgcgag ctgctgcggc tcaacgccga gcagcgcagc ctgctgcgcg atctggagcg
     1201 catcgccacc gcccgctatg ccgacggcct caccgcccag tcggatgcga tccgggcgca
     1261 gaccgaacgc acgcagctcg aagccgagcg cgtgcggctc gaagccgagc gccgccggct
     1321 cggggcgcag ctcaacgcgc tgctcgggcg ccggccggat gcgccgctcg ccgatccggt
     1381 cgcgctgtcg ccgctgccgg cgccggccgg cttcgacggc ctgcagccgc ggctgctcgc
     1441 cgccaatccg cgcctcgccg cgcaggcggc ggcggtcgag gcggccgggc acacgcgcga
     1501 actcgcgctc gcgaagcgcc tgccggatgt cacgttcggc gtctcgccgg tgcagcgcgg
     1561 cagccggctc gatggctggg aagtcatgct cgcgatcaac atcccgctcg atcaggacgc
     1621 gcgccgcgcc gacgtgcagg cgagtgccgc gcggctcgcc gcggccgagg ccgggcaggt
     1681 cgcgctcgcg ctcgagctac agcaaagcct cgccggcacg cttgccgcct acgaggccgc
     1741 cggcgaacag gaacgcctgc tgcgcacgcg gctgcggccg caggccgagg cggcgctgcg
     1801 ctcggcgctc gccggctacc gcagcgggcg cgacgacttc acgacgctgc tcgacaccct
     1861 gctcttgctg accattccgt cggtcggcgt c
//
LOCUS       DAOQJH010000610         1870 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_52448_length_1870_cov_3.057851, whole genome shotgun sequence.
ACCESSION   DAOQJH010000610 DAOQJH010000000
VERSION     DAOQJH010000610.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1870)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1870)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1870
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52448_length_1870_cov_3.057851"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1839)
                     /locus_tag="PLQ18_12380"
     CDS             complement(<1..1839)
                     /locus_tag="PLQ18_12380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02494.1"
                     /translation="MLRVAGRAREAVLGTALPPGFVPMALDSPLPARLEPDGRLRVQL
                     QPGIWTIAIEARAAGATPPERLAPPAQDEPWPEAEVWAWAARPELRTVSLRGAQPIDP
                     QQTTLPPDWRRLPVFVLGGERPALELLERARGEAETPPDRLTLVRTLWLDFDGGGWTA
                     QERLRGSIARSDRLDAAPPYAPGRVTIGGEDQFITKRKEAAGIELRAGRLDLAATGRL
                     ESAAHAGRVTLPAVGWRIEPQSLSTALQLPPGWRLLGAEGPARADGAWLARWNLLDFF
                     LLLLTTLAIGRLAGAAYGGLALVGLALVWHEPQAPRALWLNLAAALALLHVLPPGRLA
                     KLAGLWRALGLAALGLACLVYAVPQVRGALYPQLAVPDGTRATSVADEGFAAGGLAGN
                     ALTLNAPAAAPPPAPAAPVVQSELADEAGAGSDERTARERAMREYEKRKSERAMKSQD
                     SSSAPRRQQQVERYTADVQVQTGPGQPDWRWSSARLDWSGPVAPEATLTLWLLSPPLA
                     AAWRLTGVLLLFVLLWRLARCRPRPPAAAAPVEPAPIPSAAAPALLLLAALGLAGLPR
                     PAAADFPPDHLLEELRTRLSEPPACAPACAMLEHLRLQLDPARLAIT"
BASE COUNT          226 a          700 c          744 g          200 t
ORIGIN      
        1 ggtgatggcc agacgggccg gatcgagctg cagccgcagg tgttcgagca tcgcgcaggc
       61 gggcgcacag gccgggggtt ccgacaggcg cgtgcgcagt tcttcgagca gatgatcggg
      121 cgggaagtcg gccgcggccg ggcgcggcag gccggcgagg ccgagtgcgg cgagcagcag
      181 cagcgccggg gcggcggcag acgggattgg tgccggttcg accggtgccg ccgcagccgg
      241 cgggcgcggc cggcagcgcg cgagccgcca cagcagcacg aacagcagca gcacgccggt
      301 gagccgccag gcggcggcga gcggcggcga cagcagccac agcgtcagcg tcgcctccgg
      361 tgcgaccgga ccgctccagt cgagccgcgc gctgctccag cgccagtccg gctggccggg
      421 gccggtctgc acctgcacat cggcggtgta gcgctcgacc tgctgctgcc ggcgcggagc
      481 cgacgaggag tcctgcgatt tcatcgcgcg ctcgctcttg cgcttctcgt attcgcgcat
      541 cgcccgttcg cgggccgtgc gctcgtcgct gccggcgccg gcctcgtcgg ccagctcgga
      601 ctggaccacc ggcgcggccg gtgccggcgg cggggcggca gccggtgcgt tcagcgtcag
      661 cgcgttgccg gcgaggccgc cggcggcgaa accctcgtcg gcgacggacg tggcgcgcgt
      721 gccgtcgggc acggcgagct gcggatacag cgcgccgcgc acctgcggca cggcgtagac
      781 gaggcaggcg agcccgagtg ccgccagccc gagtgcgcgc cagaggccgg cgagcttcgc
      841 cagccgcccg ggcggcagca cgtgcagcag cgcgagcgcg gcggcgaggt tcagccacag
      901 cgcgcgcgga gcctggggtt cgtgccagac cagcgccagt ccgacgagcg ccagcccgcc
      961 gtaagccgca ccggcgagcc ggccgatcgc gagcgtcgtc agcagcagca ggaagaaatc
     1021 gagcaggttc cagcgcgcga gccaggcgcc gtcggcgcgc gccggccctt cggccccgag
     1081 caggcgccag cccggcggca gctgcagcgc ggtgctgagc gactgcggct cgatgcgcca
     1141 gccgaccgcc ggcagcgtca cgcgcccggc gtgcgcggcg gattcgagcc gcccggtcgc
     1201 ggccagatcg agccggccgg cgcgcagctc gatgccggcg gcctccttgc gcttcgtgat
     1261 gaactggtcc tcgccgccga tcgtcacgcg ccccggcgca tacgggggcg cggcatcgag
     1321 gcggtcgctg cgcgcgatgc tgccgcgcag gcgctcctgc gcggtccagc cgccgccgtc
     1381 gaagtcgagc cacagcgtgc gcacgagcgt cagccggtcc ggcggggttt cggcctcgcc
     1441 gcgcgcgcgt tcgaggagtt cgagcgccgg gcgctcgccg ccgagcacga acaccggcag
     1501 ccgccgccag tcgggcggca gcgtggtctg ctgcgggtcg atcggctgcg cgccgcgcag
     1561 gctgaccgtg cgcagctccg ggcgcgccgc ccaggcccag acttcggcct cgggccaggg
     1621 ctcgtcctgc gccggcggcg ccagccgctc cggcggcgtg gcgccggcgg cgcgcgcctc
     1681 gatcgcgatc gtccagatgc cgggctgcag ctgcacgcgc agccggccgt cgggttcgag
     1741 ccgtgccggc agcgggctgt cgagcgccat cggcacgaac ccgggcggca gcgcggtgcc
     1801 gagcaccgcc tcgcgcgcgc gaccggcgac gcgcagcacg acctcggtcc gaagccgcat
     1861 cggctgctcg
//
LOCUS       DAOQJH010000611         1870 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_52451_length_1870_cov_2.945455, whole genome shotgun sequence.
ACCESSION   DAOQJH010000611 DAOQJH010000000
VERSION     DAOQJH010000611.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1870)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1870)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1870
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52451_length_1870_cov_2.945455"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1274)
                     /locus_tag="PLQ18_12385"
     CDS             complement(<1..1274)
                     /locus_tag="PLQ18_12385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020833090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine dehydrogenase"
                     /protein_id="HNE02495.1"
                     /translation="MTDPRRPLADLEPDTDFVRRHIGPDAAGQAAMLATLGVGSLATL
                     IDEVVPADIRRSDAMAIAPAVDEAAVLAELRSVAARNTVLKSLIGQGYYGTHVPPVVL
                     RNVLENPAWYTAYTPYQPEISQGRLEALLNFQTLICELSGLDIANASMLDEATAAAEA
                     MTLLQRGGRSKSATFFVADDVFAQTLAVLRTRAEPLGIRIVTGDPLSVLPGLDCFGAL
                     LQYPGANGAVRDYRALAETLHARGGLLAVAADPLALCLLAPPGAWGADVVVGSSQRFG
                     VPMGFGGPHAGFLATRDALKRVMPGRLVGVSIDSHGAAALRLALQTREQHIRREKATS
                     NICTAQVLLAVMASLYACWHGPEGLQRIAQRVHRLTAIFAAGLRGQGFTVAPEAFFDT
                     LTVATGAQTAAIHARARAAGYNLREIDDATLGL"
     gene            1590..>1870
                     /locus_tag="PLQ18_12390"
     CDS             1590..>1870
                     /locus_tag="PLQ18_12390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02496.1"
                     /translation="MWFVLLLALWAAVSLYLHARLQSHMRSTAEQMQVIAAAHARAIE
                     EWWLLHESNAGLLALAGGAHSPVHRLKLDAQRKSRANTWLQSIVLTHDYL"
BASE COUNT          275 a          642 c          684 g          269 t
ORIGIN      
        1 agcccgagcg tcgcatcgtc gatctcgcgc aggttgtagc cggcggcgcg cgcgcgggcg
       61 tggatcgcgg cggtctgcgc gccggtggcg acggtcagcg tgtcgaagaa ggcttcgggg
      121 gcgaccgtga agccctggcc gcgcaggccg gcggcgaaga tcgcagtcag ccggtgtaca
      181 cgctgcgcga tgcgctgcag gccctcgggg ccgtgccagc aggcgtacag gctcgccatc
      241 accgcgagca gcacctgcgc ggtgcagatg ttcgaggtcg ccttctcgcg gcggatgtgc
      301 tgctcgcggg tctgcagcgc cagcctgagc gccgcggcgc cgtggctgtc gatcgacacg
      361 ccgaccagac gccccggcat cacgcgcttc agtgcatcgc gcgtggcgag gaagcccgcg
      421 tgcggaccgc cgaagcccat cggcacgccg aaacgctggc tgctgccgac cacgacatcg
      481 gcaccccagg ctccgggcgg cgcgagcagg cacagcgcca gcggatcggc cgcgaccgcg
      541 agcaggccgc cgcgcgcgtg cagggtctcg gcgagcgcgc ggtagtcgcg caccgcgccg
      601 ttggcaccgg ggtattgcag cagcgcgccg aagcagtcga ggccgggcag tacgctgagc
      661 ggatcgccgg tgacgatgcg gatgccgagc ggctcggcgc gcgtgcgcag cacggcgagc
      721 gtctgcgcga acacgtcatc ggcgacgaag aaggtcgcgc tcttcgagcg cccgccgcgc
      781 tgcagcagcg tcatcgcctc ggcggcggcg gtggcctcgt cgagcatcga ggcattggcg
      841 atgtcgagac cgctcagctc gcagatgagc gtctggaagt tcagcagcgc ttcgagccgg
      901 ccctgggaga tttccggctg gtacggcgta taggccgtat accaggccgg gttctccagc
      961 acgttgcgca gcaccaccgg cggcacgtgc gtgccgtagt agccctggcc gatcagcgac
     1021 ttcagcaccg tgttgcgcgc ggcgacgctg cgcagctcgg cgagcaccgc ggcctcgtcg
     1081 acggccgggg cgatcgccat tgcatcagag cggcggatgt cggccggcac gacttcatcg
     1141 atcagcgtcg ccagcgagcc gacgccgagc gtggccagca tcgccgcctg tccggcggca
     1201 tcggggccga tgtgacggcg gacgaaatcg gtgtctggtt cgagatcggc gagcgggcgc
     1261 cgcgggtcgg tcatggtcgg aaacctcggg ggactgggaa gcggaggcga cggctgcaca
     1321 cggcaggggc cgtcactgca ggcgcggcat tgtcccggtg ccggtgcggg ccgcgcaaat
     1381 cggctgttca gccggcgtta cgccgtccgc ccggacgggg tacgcgcaaa cattcataaa
     1441 gtgtgggttt accgaatctt aaccggcggt aaggtagctg cccgtacgcc gtgattcgtc
     1501 gtacggctca cccgcgtgca acaagccccc cctcgccgat gccctctctt cctgccagcc
     1561 gacgtccacc cccgctgccc ctgatcctgg tgtggttcgt gctgctgctc gcgctgtggg
     1621 cggcggtcag cctgtacctg cacgcccgcc tgcagtcgca catgcgcagc acggcggagc
     1681 agatgcaggt gatcgcagcc gcgcacgccc gggcgatcga ggaatggtgg ctgctgcacg
     1741 agagcaacgc cggcctgctg gcgctcgccg gtggcgcgca ctcgccggtg caccggctga
     1801 agctcgacgc gcagcgcaag tcccgcgcca atacctggct gcagtcgatc gtgctgaccc
     1861 acgattacct
//
LOCUS       DAOQJH010000612         1866 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_52667_length_1866_cov_3.781888, whole genome shotgun sequence.
ACCESSION   DAOQJH010000612 DAOQJH010000000
VERSION     DAOQJH010000612.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1866)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1866)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1866
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52667_length_1866_cov_3.781888"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1160)
                     /locus_tag="PLQ18_12395"
     CDS             complement(<1..1160)
                     /locus_tag="PLQ18_12395"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNE02497.1"
                     /translation="MDFPTFFHTTTGHAPYPYQQRLATAPWPELLDVPTGLGKTAAVV
                     VAWLWRRVVNDEELPRRLVYCLPMRTLVEQTERCCHDWLRNAGLAAQVDLHVLMGGRT
                     GSDWVGQPERPAILIGTQDQLLSRALMRGYAMSRYRWPVDFALLHNDALWVYDEVQLM
                     GPGLATGAQLEAFRRAPDWPTARPGRSLWLSATLHPDWLATVDFAAAVPGLVRHGIED
                     ADRADAAVTRRLLARKRLQPAGIRLTAADARSGAYARSLAAQALATHAAGTLTLLVLN
                     TVERAQQVWKALSAQRPDADCVLLHGRFRPGDRQRQQARLDAPLPAAGRIVVATQCLE
                     AGVDVSARTLFTELAPWASLVQRCGRCNRKGEYNDTAEAAVYFNRVSNLIQLG"
     gene            complement(1284..1490)
                     /locus_tag="PLQ18_12400"
     CDS             complement(1284..1490)
                     /locus_tag="PLQ18_12400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014628928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoisovalerate dehydrogenase"
                     /protein_id="HNE02498.1"
                     /translation="MNEVIFLVEETVEGGYQARALGHSIHTQADSLPELREQVRDAVN
                     CHFDADALPALIRLHFVREEVLTP"
     gene            complement(1631..>1866)
                     /locus_tag="PLQ18_12405"
     CDS             complement(1631..>1866)
                     /locus_tag="PLQ18_12405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006748920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HNE02499.1"
                     /translation="HFSPDRARWIADEHWHDDQHGHWLDDGRYELHVPYSDPTELLMD
                     VLRHVPDVEVIAPQDLRSAFVQRLRAGLERHAR"
BASE COUNT          269 a          650 c          671 g          276 t
ORIGIN      
        1 ccgagctgga tgaggttcga gacccgattg aagtagacgg cggcctcggc cgtgtcgttg
       61 tactcaccct tgcggttgca gcgcccgcag cgctgcacca gcgaggccca cggcgcgagt
      121 tcggtgaaca gcgtgcgcgc cgagacatcg actcccgctt ccaggcactg cgtcgcgacc
      181 acgatgcgcc cggccgccgg cagcggcgca tcgagccgcg cctgctggcg ctggcggtcg
      241 ccgggccgga agcggccgtg cagcagcacg cagtcggcgt ccggccgctg cgcgctcagc
      301 gccttccaga cctgctgggc gcgctcgacg gtgttcagca ccagcagcgt cagcgtgccg
      361 gcggcgtgcg tggcgagcgc ctgcgcggcg aggctgcgcg cataggcgcc ggagcgggcg
      421 tcggcggccg tcagccggat gccggccggc tgcaggcgct tgcgggccag cagccgccgg
      481 gtgacggctg catcggcgcg gtccgcatcc tcgatgccgt gccggaccag gccgggcacg
      541 gcggcggcga aatcgacggt cgcgagccag tccggatgca gcgtcgccga cagccacagg
      601 ctgcggcccg ggcgggcggt cggccagtcg ggggcgcggc ggaaggcttc gagctgcgcg
      661 ccggtcgcca ggcccggacc catcagctgc acttcgtcgt agacccacag cgcatcgttg
      721 tgcagcagcg cgaaatcgac cggccagcgg tagcggctca tcgcgtagcc gcgcatcagt
      781 gcgcgcgaca gcaactgatc ctgcgtgccg atcaggatcg ccggccgctc gggctgcccg
      841 acccagtccg aacccgtccg gccgcccatc agcacatgca gatcgacctg agcggccagc
      901 ccggcattgc gcagccagtc gtggcagcag cgttcggtct gctcgaccag cgtgcgcatc
      961 ggcaggcagt acaccagccg ccgtggcagc tcctcatcgt tcacgacccg ccgccacagc
     1021 cacgcgacca cgaccgccgc ggtcttgccg aggccggtcg gcacatcgag caactccggc
     1081 cacggggcgg tggcaagccg ctgctggtag gggtaggggg cgtggccggt ggtggtgtgg
     1141 aagaaggtgg ggaagtccat ggcgtccgtt ccggggccgg tcacgatccg gccactgtta
     1201 ctgggtgtgg ggtcaattac cgagccttgg gtgagtaccg gacgtgtgcg tgatgatctg
     1261 tccgctcggc tcgcgcggca gcttcacggc gtcagcactt cctcgcgcac gaagtgcagc
     1321 cggatcagcg ccggcagcgc gtcggcatcg aagtggcagt tgacggcatc gcgcacctgt
     1381 tcgcgcagtt cgggcagcga gtcggcctgg gtatggatcg aatgcccgag cgcccgggcc
     1441 tgatagccgc cctcgaccgt ttcctcgacc agaaagatga cttcgttcat cgtcgtggcc
     1501 cgcctgaatt ttccggctga caacagcgta tgtcgcaagg cgcaaggccg gcaggcaggg
     1561 agccatgcgg cgggccgaag gtgccggtct ccacgatcgg tcgctgcgca cccggccctc
     1621 cggccttctc tcagcgcgca tgcctttcca gccccgcccg cagccgctgc acgaaggccg
     1681 agcgcaagtc ctgcggtgcg atcacctcca cgtccggcac atgccgcagc acgtccatca
     1741 gcagttcggt cgggtcgctg tagggcacgt gcaactcgta gcgcccgtcg tccagccagt
     1801 ggccgtgctg gtcgtcgtgc cagtgttcgt cggcgatcca gcgggcgcgg tcggggctga
     1861 agtgca
//
LOCUS       DAOQJH010000613         1864 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_52774_length_1864_cov_3.721946, whole genome shotgun sequence.
ACCESSION   DAOQJH010000613 DAOQJH010000000
VERSION     DAOQJH010000613.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1864)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1864)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1864
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52774_length_1864_cov_3.721946"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..983)
                     /locus_tag="PLQ18_12410"
     CDS             complement(<1..983)
                     /locus_tag="PLQ18_12410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02500.1"
                     /translation="MFDFLRTLFTGDETPSFDDVAGLDTAIARIIAGTDPRLKLLPDH
                     RERLAPAAAAALEHARACVAALAAPHRLDREAFARDPLIHACFGSADDIGRTINEDPT
                     IRDWLATPHGHAAPALYVLLALTCEERGSFGVQLDNGVLHRDVPQTVVNFTRPVFLAP
                     AANEAGCREAVVWIVFDYCVQRALERLTELRERREELETRYRLHLGKLRALDRAHRGL
                     GAIAGQGSNDADRSAAEQELADIEEQLQAAVADTATLDDYLTRIVEVLSAPEALITVA
                     HRQLRLDRVGIRLDDPNLPGDDVDFEEATFAQGLRRVPVLVRIPGVDLEAPA"
     gene            complement(1029..1685)
                     /locus_tag="PLQ18_12415"
     CDS             complement(1029..1685)
                     /locus_tag="PLQ18_12415"
                     /inference="COORDINATES: protein motif:HMM:NF024986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HNE02501.1"
                     /translation="MNWYGCAQPGADLGNIDLRGAVLEYTDFSAARFGGAQLEDADFG
                     RSRLAAADFTRATLSNARFVAATLDAANFSGARLDGARLERAQAAGANFTGADLSAAN
                     AYQAQLGKAVFDGARLDGARLEQAVLDDARFDGASLRSARLQRASAERTRFTGADLAG
                     ARLDGARLDGADFSGADLTGASLVGARIDGARFDGARLGDCIWTDRRRCRPGSVGQCD
                     "
BASE COUNT          267 a          605 c          742 g          250 t
ORIGIN      
        1 gccggcgctt cgagatcgac cccgggaatg cgcaccagca ccggcacgcg ccggagtccc
       61 tgggcgaagg tggcttcctc gaagtccacg tcgtcgccgg gcaggttcgg gtcgtcgagg
      121 cggatgccta cgcggtcgag tcgcagctgg cggtgtgcga ccgtgatcag ggcttccggg
      181 gcggacaaca cctcgacgat gcgcgtcagg tagtcatcga gcgtcgccgt gtcggcgact
      241 gcggcctgca gctgctcctc gatgtcggcg agttcctgct cggcggcgct gcggtcggcg
      301 tcgttgctgc cctggccggc gatggcgccg aggccgcggt gggcgcggtc gagcgcgcgc
      361 agcttgccga ggtgcaggcg gtagcgggtt tccagttcct cgcgccgctc gcgcagctcg
      421 gtcaggcgct ccagcgcgcg ctgcacgcag tagtcgaaca cgatccagac cacggcctcg
      481 cgacagccgg cttcgttcgc ggccggggcg agaaacaccg gacgggtgaa gttgacgacg
      541 gtctgcggca cgtcgcggtg cagcacgccg ttgtcgagct gcacgccgaa gctgccgcgt
      601 tcctcgcagg tcagggccag cagcacgtac agcgccggcg cggcgtggcc gtgtggcgtg
      661 gccagccagt cgcggatggt cgggtcttcg ttgatcgtgc ggccgatgtc atcggccgag
      721 ccgaagcagg cgtggatcag cggatcgcgg gcgaaggctt cgcgatcgag ccggtgcggg
      781 gcggccagcg ccgcgacgca ggcgcgcgcg tgttcgagcg cggccgcagc ggccggcgcc
      841 aggcgctcgc gatgatcggg cagcagcttc aggcgcggat cggtaccggc gatgatccgg
      901 gcgatcgcgg tatccagccc ggcgacatcg tcgaaggacg gcgtctcgtc accggtgaac
      961 agcgtgcgca aaaagtcgaa catggcggcg gctccgctcg ggcaggggga aatccggggc
     1021 aggggcgctc agtcgcactg gccgaccgag ccgggacggc aacggcggcg atcggtccag
     1081 atgcagtcgc cgagtctggc gccgtcgaag cgtgcgccgt cgatgcgtgc gccgaccagc
     1141 gaggcgccgg tcagatcggc cccggagaaa tcggcgccgt cgagccgggc gccatcgagg
     1201 cgcgcaccgg cgagatcggc gccggtgaag cgggtgcgct cggcgctggc acgctgcagg
     1261 cgcgcggaac gcaggctggc gccgtcgaag cgtgcgtcat cgagcacggc ttgttcgagc
     1321 cgggcgccgt cgaggcgggc gccatcgaac acggccttgc cgagctgggc ctgataggcg
     1381 ttggcagcgc tgagatcggc accggtgaag ttcgcgccgg cggcctgcgc gcgttcgagc
     1441 cgggcgccgt cgagccgtgc gccgctgaag ttcgcagcat cgagcgtggc cgcgacgaag
     1501 cgggcgttgc tgagcgtggc cctggtgaaa tcggcggcgg cgaggcggct gcggccgaaa
     1561 tcggcatctt cgagctgcgc accgccgaag cgggctgcgc tgaagtcggt gtactccaga
     1621 accgcgccgc gcaggtcgat attgccgaga tcggctcccg gttgcgcaca gccgtaccag
     1681 ttcacgcgcc gggccggcgc atcggtgcag gccgcggacg cgatgccggc agtcagcgca
     1741 agtgcaagcg cgacgacgca cggcagtacg ccggcgggcc gggacggggg cgggacggtc
     1801 aggacaggct gcgggcacgc aaacatggct cgggcacacg gtggcgggcg gatcggtcgc
     1861 gcag
//
LOCUS       DAOQJH010000614         1863 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_52834_length_1863_cov_3.169801, whole genome shotgun sequence.
ACCESSION   DAOQJH010000614 DAOQJH010000000
VERSION     DAOQJH010000614.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1863)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1863)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1863
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52834_length_1863_cov_3.169801"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..815
                     /locus_tag="PLQ18_12420"
     CDS             <1..815
                     /locus_tag="PLQ18_12420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011979208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CheB methylesterase domain-containing protein"
                     /protein_id="HNE02502.1"
                     /translation="DLLLVPGGWGTRALLERASAELRRGADLDQLAARLAEQACAAAR
                     RVSSSAAQPVERLIAVGASTGGPDAIRTFLRGLNRRSPGVVIAQHMPAGFTRLFAERL
                     TQTTGLEVIEAAGNESILPGHVYIAPGHAHLTVRRAGSHFLTRLSDAPPVNDVRPSVD
                     VLFESLAGCAGEHVTGVLLTGMGRDGAQGLLAMRRAGARTFAQNEATCTVYGMPRAAV
                     RIGAAETVLPIERLAAAVLGEGAGGISPFEASPRSAVATGSFRAAVPAFGCV"
     gene            complement(733..1545)
                     /gene="nudC"
                     /locus_tag="PLQ18_12425"
     CDS             complement(733..1545)
                     /gene="nudC"
                     /locus_tag="PLQ18_12425"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF001299.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HNE02503.1"
                     /translation="MLQLPDRYVAAVPAADPAAPGWWFALQDGRLLVLRDAAGASLPF
                     GRDAPLPGSVLGLGHFDGVPCRALAVPPEAGPLPGAAFVSLRALWGQLDPELLGIAAQ
                     AAQLLTWDATHRFCGVCGTPTESRTDERARACPACGHMSYPRVSPAVMVRVTRGEEIL
                     LARSPRFAPGVWSILAGFVEAGEALEQTVTREVREEVGLEISGLRYFASQPWPFPHSL
                     MIGFTAEWSGGEIAIDGVEIIEAAWFTRSRMPGLPHEMSLSRRLIGDWLQKG"
     gene            complement(1610..>1863)
                     /locus_tag="PLQ18_12430"
     CDS             complement(1610..>1863)
                     /locus_tag="PLQ18_12430"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sodium-independent anion transporter"
                     /protein_id="HNE02504.1"
                     /translation="ESVPVMDISGVVALDSAIRRLHADGICVVLAGVRGQPAKVLARA
                     GIEEVPGRLLIEGKVSTAIWRVRRLLGLEDTASVALTEL"
BASE COUNT          248 a          697 c          684 g          234 t
ORIGIN      
        1 tcgacctgct gctcgtcccg ggcggctggg gcacgcgggc gctgctggag cgcgcctcgg
       61 cggaactgcg gcgcggcgcc gatctcgacc agctcgccgc acgcctggcc gagcaggcct
      121 gtgcggcggc gcggcgcgtc agcagcagcg ccgcccagcc ggtcgagcgg ctgatcgcgg
      181 tcggggcctc gaccggcggt ccggatgcga tccgcacctt cctgcgcggc ctgaaccggc
      241 gctcgccggg cgtcgtgatc gcgcagcaca tgccggccgg cttcacgcgc ctgttcgccg
      301 agcgcctgac gcagaccacc ggcctcgaag tgatcgaggc cgccggcaac gagtcgatcc
      361 tgcccggtca cgtctacatc gcgccgggtc acgcgcacct gaccgtgcgc cgcgccggca
      421 gccacttcct gacgcggctg tcggacgccc cgccggtcaa cgacgtgcgg ccctcggtcg
      481 acgtgctgtt cgaatcgctc gccggctgcg ccggcgagca cgtcaccggc gtgctgctga
      541 ccggcatggg ccgcgacggt gcgcaggggc tgctggcgat gcgccgggcc ggcgcgcgca
      601 ccttcgcgca gaacgaggcc acctgcaccg tctacggcat gccgcgcgcg gccgtgcgca
      661 tcggtgccgc cgaaaccgtg ctgccgatcg agcggctggc cgcggccgtg ctcggtgagg
      721 gggccggggg catcagccct tttgaagcca gtccccgatc agccgtcgcg acaggctcat
      781 ttcgtgcggc agtcccggca ttcggctgcg tgtgaaccac gcggcctcga tgatctcgac
      841 gccgtcgatc gcgatctcgc caccgctcca ttccgcggtg aagccgatca tcagcgaatg
      901 cggaaacggc cacggctggc tcgcgaagta gcgcagtccg ctgatttcca gcccgacttc
      961 ctcgcgcacc tcgcgcgtca cggtctgttc gagcgcctcg ccggcctcga cgaagccggc
     1021 gaggatcgac cagacgccgg gcgcgaagcg cggcgagcgc gcgagcagga tttcctcgcc
     1081 gcgcgtcacg cgcaccatca ccgccggcga cacgcgcgga taactcatgt ggccgcaggc
     1141 cggacaggcg cgggcgcgct cgtcggtgcg tgattcggtc ggcgtgccgc agaccccgca
     1201 gaagcggtgc gtggcgtccc aggtcagcag ctgcgcggcc tgcgcggcga tgccgagcag
     1261 ctccggatcg agctggcccc acagcgcgcg caggctgacg aaggctgcgc cgggcagcgg
     1321 cccggcttcg ggcggcacgg cgagcgcgcg gcagggcacg ccgtcgaagt ggccgaggcc
     1381 gagcacgctg ccgggcagcg gcgcgtcgcg gccgaacggc aggctggcgc cggcggcgtc
     1441 gcgcagcacc agcaggcggc catcctgcag cgcgaaccac cagcccggtg ccgccgggtc
     1501 ggcggcgggc acggcggcga cgtagcgatc gggcagttgc agcatcgggc ggctcctcgg
     1561 cgacggtgac gcggcgcatc atcgcacggc cgcggccgct ggccggggct cagagctcgg
     1621 tcagcgccac gctggcggtg tcctccagtc cgagcagccg gcgcacgcgc cagatcgccg
     1681 tcgacacctt gccctcgatc agcagccggc ccgggacttc ctcgatgccg gcacgtgcca
     1741 gcactttcgc cggctggccg cgcacgccgg cgagcacgac gcagatgccg tcggcatgca
     1801 ggcggcggat cgccgaatcg agcgcgacga cgccggagat gtccatgacc ggcaccgatt
     1861 cca
//
LOCUS       DAOQJH010000615         1861 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_52945_length_1861_cov_3.241417, whole genome shotgun sequence.
ACCESSION   DAOQJH010000615 DAOQJH010000000
VERSION     DAOQJH010000615.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1861)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1861)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1861
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52945_length_1861_cov_3.241417"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="PLQ18_12435"
     CDS             complement(<1..197)
                     /locus_tag="PLQ18_12435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02505.1"
                     /translation="MLPHPVLPPRVPTPQPRLAAGAPLRWLRAGWQVYRHTAAASSLA
                     TALPAALGGLLVAAVLAGGRAP"
     gene            465..1499
                     /gene="murB"
                     /locus_tag="PLQ18_12440"
     CDS             465..1499
                     /gene="murB"
                     /locus_tag="PLQ18_12440"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008060960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HNE02506.1"
                     /translation="MTAASVLQREVALDGLNTFGLPARAEHFLNLEADSQLAPALAEA
                     ARAQLAIHVLGAGSNVLLAGDVAGLVIRVALCGRRLLLRNGDEVVVEAGAGEDWHGFV
                     AWTLANGWFGLENLALIPGTVGGSPIQNIGAYGVEAGERIAHVDVIDLETGQARRLMP
                     SACGFGYRDSVFKHELAGRVLVVAVAFRLSLQPAVQARYAELNAELQAQGIEAPTPQQ
                     VFEAVCAVRRRKLPEPAVLGNAGSFFKNPVVDFEQATRLRAAHPELVSYPQPDGRVKL
                     AAGWLIERCGWKGRALGRAGVYERQALVLVNHGGATGAEVLALAEAIRADVRTRFGVA
                     LEMEPVRMGG"
     gene            1589..>1861
                     /locus_tag="PLQ18_12445"
     CDS             1589..>1861
                     /locus_tag="PLQ18_12445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004363758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNE02507.1"
                     /translation="MRYPERFDVIVVGGGHAGTEAALAAARCGVRTLLLTHNIETLGA
                     MSCNPAIGGIGKGHLVREIDALDGAMARATDRAGIQFRILNASKGPA"
BASE COUNT          240 a          668 c          701 g          252 t
ORIGIN      
        1 ggcgcccggc cgccggcgag cacggcggcg accagcagcc cgccgagggc cgccggcagc
       61 gccgtcgcca gactcgatgc ggcggccgtg tgccggtaga cctgccagcc ggcacgcaac
      121 cagcgcagcg gtgcgccggc ggcgagtcgt ggctgcggcg tcggcacgcg cggggggagg
      181 acggggtgcg ggagcatcgc tgcgggcctc gggagacggg ggcggaaatg ccgtcgaggc
      241 tggccggtgc gcgggcgcct ggccttgatc cggattaaac gcttggtaac ctgcggcttg
      301 ggcgcgctgc cggccggccc tacaatcgcc ggcctgcgac cgagggagcc tgaccgagcc
      361 gtgatgccga tcacacctac ctgcatggtt ttctccccct ctccccggtc cctctcccgc
      421 acggggcgag gggccgaaaa gcgaagcgct gcggcgtgag cggcatgacg gccgcatccg
      481 tgctgcagcg cgaagtcgcg ctcgatggcc tcaacacctt cgggctgccg gcccgggccg
      541 agcatttcct gaacctcgaa gccgacagcc agctcgcgcc ggcgctcgcc gaggccgcgc
      601 gcgcgcagct cgcgatccac gtgctcggtg ccggcagcaa cgtgctgctc gccggcgacg
      661 tggccgggct ggtgatccgc gtcgcgctgt gcggccgccg cctgctgctg cgcaacggcg
      721 acgaggtcgt cgtcgaggcc ggcgccggcg aggactggca cggcttcgtg gcctggacgc
      781 tcgccaacgg ctggttcggc ctcgaaaacc tggcgctgat ccccggcacg gtcggcggca
      841 gcccgatcca gaacatcggt gcctacggcg tcgaggccgg cgagcgcatc gcgcacgtcg
      901 acgtgatcga tctcgaaacc ggccaggcgc gccggctgat gccttcggcc tgcggcttcg
      961 gctaccgcga cagcgtgttc aagcacgagc tggccggccg ggtgctggtg gtggcggtgg
     1021 ccttccggct gtcgctgcag ccggccgtgc aggcgcgcta cgccgagctc aacgccgagc
     1081 tgcaggcgca gggcatcgag gcgccgacgc cgcagcaggt gttcgaggcc gtctgtgcgg
     1141 tgcgccggcg caagctgccg gagccggccg tgctcggcaa cgccggcagc ttcttcaaga
     1201 acccggtcgt cgatttcgag caggcgacgc gcctgcgcgc cgcgcatccg gagctggtca
     1261 gctatccgca gccggacggc cgggtcaagc tcgccgccgg ctggctgatc gagcgctgcg
     1321 gctggaaggg ccgcgcgctc gggcgcgccg gcgtctacga gcgtcaggcg ctggtgctgg
     1381 tcaaccacgg cggcgccacc ggggccgagg tgctggcgct cgccgaggcg atccgcgccg
     1441 acgtgcgcac gcgcttcggc gtcgcactgg agatggagcc ggtgcggatg ggcggctgac
     1501 gacgcggctg cgccccgtgc gcgccggcgt ggccggtgct acgatccgcc gccgttttcc
     1561 ttcctgtttt cactggcgga tcaagtccat gcgctatccg gaacgcttcg atgtcatcgt
     1621 cgtcggcggc ggtcacgccg gcaccgaggc cgcactcgcg gccgcgcgct gcggcgtgcg
     1681 cacgctgctg ctgacgcaca acatcgagac cctgggggcg atgagctgca acccggcgat
     1741 cggcggcatc ggcaagggac acctcgtgcg cgagatcgac gcgctcgacg gcgcgatggc
     1801 gcgcgccacc gaccgcgccg gcatccagtt ccgcatcctc aacgccagca agggcccggc
     1861 c
//
LOCUS       DAOQJH010000616         1860 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_52983_length_1860_cov_7.703601, whole genome shotgun sequence.
ACCESSION   DAOQJH010000616 DAOQJH010000000
VERSION     DAOQJH010000616.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1860)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1860)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1860
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52983_length_1860_cov_7.703601"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            173..604
                     /gene="mscL"
                     /locus_tag="PLQ18_12450"
     CDS             173..604
                     /gene="mscL"
                     /locus_tag="PLQ18_12450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005794217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HNE02508.1"
                     /translation="MSLVQEFKAFAMKGNVIDLAVGVIIGAAFNKIVESVVGDLIMPI
                     LAFILGGKLDFSNMFFVLGTVPPGTAQTLDALKKAGVPVFAYGSFITITVNFIILAFI
                     IFMMIKQVNRLRAAEPVAPEPEPVTPEDIVLLREIRDSLKK"
     gene            complement(693..1241)
                     /locus_tag="PLQ18_12455"
     CDS             complement(693..1241)
                     /locus_tag="PLQ18_12455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02509.1"
                     /translation="MRRTLLALALAAPLTALAGPTIDKISATPSPSKVGEAITITIEA
                     SEAENGMCGLSLNWGDGNKMPPKKVGGNHKNFPLTMQHTYAKPGTYEIKADGARAETY
                     LPCMGKVKYMHTVEGAPAKAGAATSACPEGWGQKGKTAKDGSFTCTPKAKGAKKPEKE
                     LACPAGTSYFYGAKALGCEKAQ"
     gene            complement(1319..>1860)
                     /locus_tag="PLQ18_12460"
     CDS             complement(1319..>1860)
                     /locus_tag="PLQ18_12460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional glutamine synthetase
                     adenylyltransferase/deadenyltransferase"
                     /protein_id="HNE02510.1"
                     /translation="SVESFREYELKNAWVWEHQALTRARFIAGDPAVGARFEAIRQEI
                     LTQQRDSAKLREEVLSMRQRMLDNHASNSETEFDLKQDPGGIIDVEFIVQYLILAHAH
                     AHPELTRNLGNIALLGMSADLGLIPAELADPVRNAYREYRRMQHAARLNDEKSRVERA
                     AVLRRIEAVRALWQHVFGT"
BASE COUNT          290 a          577 c          589 g          404 t
ORIGIN      
        1 aatcaacgcc aacagcatca accagtccga ttttatcttc gcctgaggcg cgcgtttccg
       61 gctttcacgg ggcccgttgc ggccccgttt tcattgcggc gtgcctgcct gaacggcgtg
      121 aaaacgtcat acaatgcccg taccccaatc atttcacaga caaggagggc ccatgagcct
      181 cgtacaggaa ttcaaggcgt tcgcgatgaa gggcaacgtc atcgatctcg ccgtcggcgt
      241 gatcatcggc gcggccttca acaagatcgt cgagtcggtc gtcggcgacc tgatcatgcc
      301 gatcctcgcc ttcatcctcg gcggcaagct cgatttctcg aacatgttct tcgtgctcgg
      361 caccgtgccg ccgggtaccg cacagacact cgatgcgctg aaaaaagccg gcgtacccgt
      421 gttcgcctac ggcagcttca tcaccatcac ggtcaatttc atcatcctcg ccttcatcat
      481 cttcatgatg atcaagcagg tgaatcgcct gcgtgctgcc gagcccgtgg cgccggagcc
      541 ggagccggtg acgccggagg acatcgtcct gctgcgcgag atccgcgaca gcctgaagaa
      601 gtaggaccgc atccgcaaaa ggaaaacggc gcccgcgggc gccgtttttg ctgttgccga
      661 cggcttcgtc ggcccgggat ccgccaactc gcctactgtg ccttttcgca gcccagtgcc
      721 ttggcgccgt agaagtagct ggtccccgcc gggcaggcca gttccttctc cggcttcttc
      781 gcccccttgg ccttcggcgt gcaggtgaag ctgccgtcct tggcggtctt gcccttctgt
      841 ccccagcctt ccggacaggc gctggttgcg gcgccggcct ttgctggcgc tccttccacc
      901 gtatgcatgt acttcacctt gcccatgcag ggcaggtagg tctcggcgcg tgcgccgtcg
      961 gccttgatct cgtaggtgcc gggcttggcg taggtgtgct gcatcgtgag cgggaagttc
     1021 ttgtggttgc cgcccacctt cttgggcggc atcttgttgc cgtcgcccca gttcagcgag
     1081 aggccgcaca tgccgttctc cgcttcgctg gcctcgatgg tgatggtgat cgcttcgccg
     1141 accttcgagg gcgatggcgt tgcgctgatc ttgtcgatcg tcgggccggc gagtgcggtc
     1201 agcggagcgg caagggcgag ggcaagcagg gtacggcgca tggtctctct cctgagtggt
     1261 tggaaattcc agtatggctc atgtacggac aaaagactgt cagaattttg tgtcattgtc
     1321 atgttccgaa gacgtgctgc cagagtgcac ggacggcctc gatccggcgc agcaccgccg
     1381 cgcgttcgac gcgtgacttc tcgtcgttca ggcgtgcggc gtgctgcatg cggcggtact
     1441 cgcgataggc attgcggacc ggatcggcca gttcggccgg gatcaggccg agatcggcgc
     1501 tcatgccaag gagggcgatg ttgcccaggt tgcgcgtcag ttcgggatgg gcgtgggcat
     1561 gggcgaggat caggtattgc acgatgaatt cgacgtcgat gatgccgccc ggatcctgct
     1621 tgaggtcgaa ttccgtctcg ctgttcgagg cgtgattgtc gagcatgcgc tggcgcatcg
     1681 acagcacttc ctcgcgcagc ttcgcgctgt cgcgttgctg cgtcaggatt tcctggcgga
     1741 ttgcctcgaa acgcgcgccg acggcggggt cgccggcgat gaagcgggcc cgcgtcagcg
     1801 cctgatgctc ccagacccag gcgttcttca gctcgtattc gcggaaggat tcgacgctgt
//
LOCUS       DAOQJH010000617         1854 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_53311_length_1854_cov_4.295164, whole genome shotgun sequence.
ACCESSION   DAOQJH010000617 DAOQJH010000000
VERSION     DAOQJH010000617.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1854)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1854)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1854
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53311_length_1854_cov_4.295164"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1854)
                     /locus_tag="PLQ18_12465"
     CDS             complement(<1..>1854)
                     /locus_tag="PLQ18_12465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HNE02511.1"
                     /translation="PPLAQTPPVEPQPPVAEPPRTQDAPVASGNRPLLIPGKKTLFQR
                     VVLGPGAALFDAPGGRQLHALDPFAIYYVYERRSVGGGTWLRIGGASDGRAEGWMDGS
                     KGYDWRQTLTLKFTERSGRDPVMFFGERAPLENLFVQSDARQVVRDWQKKIAAAKGSA
                     VPGVPVVALEPAATAIADQRFYLLPIFDHTETFGADGQPLSLLQVATVDPGAAAPPAA
                     APPAADAFRTGVVFVFDTTRSMNPYLDRARTAIRKIYDRLAREKLTDKVGIGLVAFRS
                     SVTKTPGLEYVSRVFEPLSPATTPAQVMSDAQQIVATEVSSHSFNEDAFAGVMDAVER
                     MDWKPYGARVAILITDAGALRKNDPLGATQMNEAEVRAAALAKGVHLIVLHLKTPAGR
                     NNHASAETQYRTLAADPNPQIGSLYVPVSGGDVGAFGDAVETIVGKLVDIVRDTAAGQ
                     DAPAPDIDAGYAGVADKTAALGYALRMDFLGAKTAARPPGVVSAWVADRDPARPDVPS
                     FQVCVLLTKLQLNDLQQGLKTIVESARRAQKSPKDFFQEVASASASFTRGQETKTPRN
                     LYATGLLGEFLDGLPYKSKVLNLTEDLWLSWSVAEQEDFIQELESKIRLYET"
BASE COUNT          251 a          624 c          688 g          291 t
ORIGIN      
        1 gtctcgtaca ggcggatctt cgattccagc tcctggatga agtcctcctg ctcggcgacg
       61 ctccacgaca gccacaggtc ttcggtcagg ttcagcacct tgctcttgta cggcaggccg
      121 tcgaggaatt cgccgagcag gccggtggcg tacaggttgc gcggggtctt cgtctcctgg
      181 ccgcgcgtga agctggcgct ggccgaggcg acctcctgga agaagtcctt cgggcttttc
      241 tgcgcgcggc gtgcggactc gacgatggtc ttcaggccct gctgcaggtc attgagctgc
      301 agcttggtca gcagcacgca gacctggaag ctcggcacgt cgggccgcgc cggatcgcgg
      361 tcggcgaccc aggccgagac cacgcccggc ggccgggcgg cggtcttggc gccgaggaag
      421 tccatgcgca gcgcgtagcc gagggcggcg gtcttgtcgg cgaccccggc gtaaccggca
      481 tcgatgtccg gcgccggggc gtcctgaccg gcggcggtgt cgcgcacgat gtcgacgagc
      541 ttgccgacga tggtctcgac cgcatcgccg aaggcaccga catcgccgcc gctgaccggc
      601 acgtacagcg agccgatctg cgggttcggg tcggcggcga gcgtgcggta ctgggtttcg
      661 gccgaggcgt ggttgttgcg cccggccggc gtcttcaggt gcagcacgat caggtgcacg
      721 cccttggcga gcgcggcggc gcgcacctcg gcctcgttca tctgcgtggc gccgagcgga
      781 tcgttcttgc gcagcgcccc ggcgtcggtg atcaggatcg cgacgcgcgc gccgtagggc
      841 ttccagtcca tgcgctcgac ggcatccatg acgccggcga aggcatcctc attgaaggag
      901 tggctggaaa cctcggtcgc gacgatctgc tgcgcatcgc tcatgacctg cgccggcgtg
      961 gtggcgggcg acagcggctc gaacacgcgc gacacgtatt cgaggccggg cgtcttcgtg
     1021 acgctgctgc ggaaggcgac gaggccgatg ccgaccttgt cggtcagctt ctcgcgcgcc
     1081 aggcggtcgt agatcttgcg gatcgcggtg cgggcgcggt cgaggtaggg gttcatcgag
     1141 cgcgtcgtgt cgaacacgaa cacgacgccg gtgcggaagg catcggcagc cggcggcgcg
     1201 gcggccggcg gcgccgcggc gcccggatcg acggtcgcga cctgcagcag cgacagcggc
     1261 tggccgtcgg cgccgaaggt ctcggtgtga tcgaagatcg gcagcaggta gaagcgctgg
     1321 tcggcgatcg cggtcgcggc cggctccagc gccacgaccg gcacgccggg cacggcactg
     1381 cctttcgccg cggcgatctt cttctgccag tcgcgcacca cctgccgcgc atcggactgc
     1441 acgaacaggt tttcgagcgg tgcgcgctcg ccgaagaaca tcaccgggtc acggccgctg
     1501 cgctcggtga acttcagcgt cagcgtctgg cgccagtcgt agcccttgct gccgtccatc
     1561 cagccctcgg cgcggccgtc gctggcaccg ccgatgcgca gccaggtgcc gccgccgacg
     1621 ctgcgccgct cgtagacgta gtagatcgcg aacggatcga gcgcatgcag ctgccggccg
     1681 ccgggcgcat cgaagagcgc cgcgccggga ccgagcacga cgcgctggaa cagcgtcttc
     1741 ttgccgggga tcagcagcgg gcggttgccg ctcgcgaccg gagcgtcctg cgtccgcggc
     1801 ggctcggcga ccggcggctg cggctcgacc ggcggggtct gcgcgagcgg cggt
//
LOCUS       DAOQJH010000618         1853 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_53369_length_1853_cov_4.068966, whole genome shotgun sequence.
ACCESSION   DAOQJH010000618 DAOQJH010000000
VERSION     DAOQJH010000618.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1853)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1853)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1853
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53369_length_1853_cov_4.068966"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="PLQ18_12470"
     CDS             complement(<1..186)
                     /locus_tag="PLQ18_12470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02512.1"
                     /translation="MIAASLHALLRFALTWLVAGVLSASTATGLAIALVLPLALSVAG
                     RLPRMRPVRRAPPVVRSG"
     gene            complement(290..1183)
                     /gene="argB"
                     /locus_tag="PLQ18_12475"
     CDS             complement(290..1183)
                     /gene="argB"
                     /locus_tag="PLQ18_12475"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011536400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HNE02513.1"
                     /translation="MALTADAAMNVAHILSEALPYIQRYRGKTIVIKFGGNAMETEDV
                     QSSFARDVVLMKTVGINPVVVHGGGPQIGMLLKALGKESRFVDGLRVTDAETMDVVEM
                     VLGGLVNKQIVSLINRHGGKAVGLSGKDADLIRARKLEYSRKTPEMDAPEIIDLGHVG
                     EVQSIDTSVIEMLVRGDFIPVIAPTGVGPDGRAYNINADTVAGKVAEALRAEKFMLLT
                     NISGILDKEGKLLTGLSARQVDDLIGDGTIYGGMLPKVRCALDAVRGGVRTSLILDGR
                     VPHSVLVELFTDEGIGTLIRG"
     gene            complement(1239..>1853)
                     /locus_tag="PLQ18_12480"
     CDS             complement(1239..>1853)
                     /locus_tag="PLQ18_12480"
                     /inference="COORDINATES: protein motif:HMM:NF014879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HNE02514.1"
                     /translation="GEIVWPDRVMMALAAGVLCAQPGATIVFDVKCSSRLGALIERLG
                     GHAECWKTGHSLIKARRAELAAPFAGEYSGHLCFADWYGLDDALYAALRLVTLLVADG
                     RDSDALFADHPVGVSTPELRIACADPHALAAQLQTLDWEGARRVTIDGVRAEWPDGWA
                     LVRASNTESCLVLRLEADDADTLAAIRARVAARLAAVDPALALP"
BASE COUNT          279 a          644 c          651 g          279 t
ORIGIN      
        1 gccgctgcgc acgaccggcg gcgcgcggcg caccggacgc atccgcggca gccggccggc
       61 gaccgacagc gcgagcggca gcaccagcgc gatcgcgagc ccggtcgccg tgcttgcact
      121 cagcacgccg gcgaccagcc aggtcagcgc gaaacgcagg agggcatgca ggctggcggc
      181 gatcatggcg gcggtcctcg gcgggctgga tatgcgccca gactggaccg ccaccgcgtt
      241 tgaatgaaat tgattctcga caaaccggcg ataggccgct gctatgccct cagccgcgga
      301 tcagcgtgcc gatgccctcg tcggtgaaca gctccaccag caccgagtgc ggcacgcggc
      361 cgtcgaggat cagcgaggtc ctgacaccgc cgcgcaccgc gtcgagcgcg cagcgcacct
      421 tcggcagcat gccgccgtag atcgtgccgt cgccgatcag gtcatcgacc tggcgcgccg
      481 acaggccggt cagcagcttg ccctccttgt cgaggatgcc gctgatgttg gtcagcagca
      541 tgaacttctc ggcgcgcagc gcctcggcga ccttgccggc gaccgtgtcg gcgttgatgt
      601 tgtacgcgcg cccgtccggg ccgacgccgg tcggcgcgat gaccgggatg aagtcgccgc
      661 gcaccagcat ctcgatgacc gaggtgtcga tcgactgcac ctcgccgacg tggccgaggt
      721 cgatgatctc gggcgcgtcc atctccgggg tcttgcggct gtattcgagc ttgcgcgcgc
      781 ggatcaggtc ggcgtccttg ccgctcaggc cgacagcctt gccgccgtgg cggttgatca
      841 ggctgacgat ctgcttgttg acgaggccgc cgagcaccat ctcgaccacg tccatggtct
      901 cggcatcggt gacgcgcagg ccgtcgacga agcggctttc cttgccgagc gccttcagca
      961 gcatgccgat ctgcggaccg ccgccgtgca cgacgaccgg gttgatgccg acggtcttca
     1021 tcagcaccac gtcgcgcgcg aagctcgact gcacgtcctc ggtttccatg gcgttgccgc
     1081 cgaacttgat gacgatggtc ttgccgcggt agcgctggat gtacggcagg gcttcggaaa
     1141 ggatgtgggc gacgttcatc gccgcatcgg cggtcagggc catcgcttcg gtacctcggc
     1201 ggacgttcgg aagaggacag cggcggcgcg cggcgcgttc agggcagcgc cagcgccgga
     1261 tcgacggcgg cgaggcgcgc ggccacgcgg gcgcggatcg cggcgagtgt atcggcatcg
     1321 tcggcttcga gccgcagcac gaggcaggat tcggtgttcg aggcgcgcac cagcgcccag
     1381 ccatccggcc attcggcgcg cacgccgtcg atcgtcaccc gccgtgcgcc ctcccagtcg
     1441 agcgtctgga gctgcgcggc cagggcatgg ggatcggcgc aggcgatgcg cagttcgggc
     1501 gtcgacacgc caaccgggtg atcggcgaac agcgcatcgc tgtcgcggcc atcggcgacg
     1561 agcagcgtca ccagccggag cgccgcgtac agcgcatcgt cgagcccgta ccagtcggcg
     1621 aagcacagat gcccgctgta ctcgccggcg aacggtgccg cgagttcggc gcgtcgggcc
     1681 ttgatcaggc tgtggccggt cttccagcac tccgcgtgcc cgccgaggcg ctcgatcagt
     1741 gcgccgagcc gggagctgca cttcacgtca aagacgatgg tcgcgcccgg ctgcgcgcac
     1801 agcaccccgg cggcgagcgc catcatcacg cggtccggcc agacgatttc gcc
//
LOCUS       DAOQJH010000619         1851 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_53507_length_1851_cov_2.523385, whole genome shotgun sequence.
ACCESSION   DAOQJH010000619 DAOQJH010000000
VERSION     DAOQJH010000619.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1851)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1851)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1851
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53507_length_1851_cov_2.523385"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1851)
                     /locus_tag="PLQ18_12485"
     CDS             complement(<1..>1851)
                     /locus_tag="PLQ18_12485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02515.1"
                     /translation="QVLPADQHLAQELALWCGEPLSAPMPDVAACRSFIEAGAAAALP
                     LFESAQEAWRQATGNPQQHLYGLPAFLHVCALLAVHGIGAIRDISKHLGYCSMSAVPA
                     ADVILGTALAALADEGAYPPASLPEEPDLTCLFDLLMRRWLGQPPVPGETAMLETIIA
                     RASAAGYGWIAAEARAVLARCRGEPAQSVLLDVVPPKPAWQRALNRLIALGDGPAARV
                     RLIWGIDRQGGRLRLELREQKPAARGGWTPGKQVELYRYETRSSQEALLPEDRAALHS
                     LGGIHDLWHDDHVVRLLGALTGHPRVYWIGKPGEWLELVEVPPRLSLRRDKSAEGGWL
                     LELLPAPKKPGTVWQLARVKNTSRVEYTVYTHEQQRLGAVLDNGRLHVPEAGRAELAR
                     TLQALDRVLPAGAALDAVLGDDDVQPGDPTPCLLLAPAGDGLRAQLLVEPLAGLARQR
                     CGEGATVLLGERDGRPLRVRRDPAAERAARAQLLEACPTLAGLEEDDTLVLPEPQAAL
                     GLLAELRTLETPAAASPAAAGDPLPETAAASGATPAAAAPAVRVVWPHGESLRLRKVL
                     HVRDLSLSLSGEGAWLQVGGSVAVDEQRVLALSALAAAALQGRYVALGNG"
BASE COUNT          261 a          698 c          669 g          223 t
ORIGIN      
        1 tcgccgttgc cgagcgcgac gtagcggccc tgcagcgcgg cggccgcgag cgccgacagc
       61 gccagcacgc gctgctcatc gaccgcgacc gaaccgccga cctgcagcca cgcgccctcg
      121 ccggacagcg acagcgacag gtcgcgcacg tgcagcacct tgcgcaggcg cagcgattcg
      181 ccgtgcggcc agacgacgcg cacggccggc gcagcagcgg ccggagtcgc gccggatgcg
      241 gcagcggttt caggcagcgg atcgcccgct gccgccggtg acgccgcggc cggcgtctcc
      301 agcgtgcgca gctcggcgag caggccgagc gcggcctgcg gctcgggcag caccagcgtg
      361 tcgtcctcct cgaggccggc gagcgtcggg caggcttcga ggagctgcgc ccgcgccgcg
      421 cgctcggcgg ccggatcgcg gcggacgcgc agcggccggc catcgcgctc gccgagcagc
      481 acggtcgctc cctcgccgca gcgctggcgc gccagcccgg cgagcggctc gaccagcagc
      541 tgcgcgcgca ggccgtcgcc ggccggcgcc agcagcaggc acggggtcgg atcgccgggc
      601 tgcacgtcat catcgccgag cacggcatcg agcgcggcgc cggccggcag cacgcgatcg
      661 agcgcctgca gcgtgcgtgc gagttcggcc cggccggcct cgggcacgtg cagccggccg
      721 ttgtcgagca cggccccgag ccgctgctgc tcatgcgtgt agacggtgta ctcgacgcgg
      781 ctggtgttct tcacgcgcgc gagctgccag accgtgcccg gcttcttcgg cgccggcagc
      841 agttcgagca gccagccgcc ttcggccgac ttgtcccggc gcagcgacag gcgcggcggc
      901 acctcgacca gttccagcca ttcgccgggc ttgccgatcc agtacacgcg cggatgtccg
      961 gtcagcgccc cgagcaggcg cacgacgtga tcgtcgtgcc acagatcatg gatgccaccg
     1021 agggaatgca gcgcggcgcg atcctcgggc agcagggcct cctgactgct gcgggtttcg
     1081 tagcggtaga gttccacctg cttgcccggc gtccagccgc cgcgcgccgc cggcttctgc
     1141 tcgcgcagtt cgagccgcaa ccggccgccc tgacggtcga tgccccagat caaccgcacg
     1201 cgggccgccg gtccgtcccc gagtgcgatc agccggttca gcgcccgctg ccaggccggc
     1261 ttcggcggca cgacatcgag cagcacggac tgcgccggct caccacgaca gcgcgcgagc
     1321 acggcccgtg cctcggcagc gatccagccg tagccggcgg ccgaggcgcg cgcaatgatc
     1381 gtttcgagca tggcggtctc gcccggcacc ggcggctggc ccagccagcg gcgcatcagc
     1441 aagtcgaaca ggcaggtgag atcgggctct tcgggcaggg acgccggcgg ataagccccc
     1501 tcatcggcca gggcagcgag cgcggtgccg aggatcacgt cggccgccgg taccgcactc
     1561 atgctgcagt agcccaggtg cttgctgatg tcccggatcg cgccgatccc gtgcacggcc
     1621 agcagcgcgc agacgtgcag aaaggccggc aagccgtaca gatgctgttg cggattgccg
     1681 gtggcctgcc gccacgcctc ctgcgccgac tcgaacaagg gcagcgccgc cgcggcacct
     1741 gcctcgatga acgagcggca cgccgccacg tcgggcatcg gtgcggaaag cggttcgcca
     1801 caccagagcg ccagctcctg ggcgaggtgc tgatcggcgg gcagaacctg c
//
LOCUS       DAOQJH010000620         1845 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_53855_length_1845_cov_3.877095, whole genome shotgun sequence.
ACCESSION   DAOQJH010000620 DAOQJH010000000
VERSION     DAOQJH010000620.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1845)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1845)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1845
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53855_length_1845_cov_3.877095"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            48..782
                     /locus_tag="PLQ18_12490"
     CDS             48..782
                     /locus_tag="PLQ18_12490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HNE02516.1"
                     /translation="MRICVINPNTSAAMTETIGRAARRVAAAGTEIVAQQPDAGPESI
                     ESHVDEAFAAVGVAELVRRGELLGFDGYVLACFGDPGLLAARELAKGPVLGIAEAAFH
                     AASMLAGRFSVVTTLARTVPIAEHLLHAYGLERRCVRVRAAEIPVLDLDTPAAYARIA
                     GECRRARDEDGIGALVLGCGGMAELAPELSRELGLPVVDGVTAAVKFVESLIALGLGT
                     GKTGDGAYPPPKRYVGRFADWTTTAC"
     gene            800..1729
                     /gene="puuE"
                     /locus_tag="PLQ18_12495"
     CDS             800..1729
                     /gene="puuE"
                     /locus_tag="PLQ18_12495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase PuuE"
                     /protein_id="HNE02517.1"
                     /translation="MSDQPYPRDLIGYGRTPPHARWPQQARIAVQFVLNYEEGGENCV
                     LHGDAGSEQFLSELFAPASFPDRHLSMESIYEYGSRVGVWRILREFERRGLPLTVFGV
                     AMALERHPEVTTAFVELGHEIACHGWRWIHYQNIDEATEREHLRIGLEVIEHLTGTRP
                     LGWYTGRDSPNTRRLVVDDGGLLYDSDYYGDDLPFWTEVTRSDGVTMPHLIVPYTLDA
                     NDMRFSLPQGFSHGDEFFEYLRDSFDVLYAEGAETPRMLSVGLHCRLIGRPGRFRALQ
                     RFLDHIERHDRVWVCRRIDIARHWHAEHPYVPG"
BASE COUNT          268 a          649 c          625 g          303 t
ORIGIN      
        1 ggcacagcct gtcctttcca tcccgatcaa cccttgaggc cagcccgatg cgcatctgcg
       61 tgatcaaccc caacaccagc gccgcgatga ccgaaaccat cggccgggcc gcccggcgcg
      121 tcgcggcggc gggcaccgag atcgtcgcgc agcagccgga tgccgggccg gagtcgatcg
      181 agagtcacgt cgatgaggct ttcgccgcag tcggcgtcgc cgaactggtg cgccgcggcg
      241 agctgctcgg ttttgatggc tacgtgctcg cctgcttcgg cgatccgggc ctgctcgccg
      301 cgcgcgaact ggcgaaaggc ccggtgctcg gcatcgccga agcggccttt cacgccgcgt
      361 cgatgctggc cgggcgtttc tcggtggtca caacgctcgc gcgtaccgta ccgatcgccg
      421 aacacctgct gcacgcctat ggtctggaac ggcgctgcgt gcgcgtgcgt gccgccgaaa
      481 taccggtgct cgatctcgac actccggcgg cctatgcgcg catcgccggt gaatgccggc
      541 gcgcgcgcga cgaggacggc atcggtgcgc tggtgctcgg ctgcggcggc atggcggagc
      601 tcgcgccgga gctgtcgcgc gaactcgggc tgccggtggt cgatggggtc acggcagccg
      661 tgaagttcgt cgagtcgctg atcgcgctcg gactcggcac cggcaagacc ggcgacggtg
      721 cctatccgcc gccgaagcgt tacgtcggcc gttttgccga ctggaccacg acggcgtgct
      781 gaatgccacg gagcctgtaa tgtccgacca gccttatccc cgcgacctga tcggctacgg
      841 ccgcacgccg ccgcacgcgc gctggccgca gcaggcgcgc atcgccgtgc agttcgtgct
      901 caattacgag gagggcggcg agaactgcgt gctgcacggc gatgccggca gcgagcagtt
      961 cctgtccgag ctgttcgcgc cggcgagctt cccggaccgg catctgtcga tggaatcgat
     1021 ctacgaatac ggctcgcgcg tcggcgtctg gcgcatcctg cgcgaattcg agcgccgcgg
     1081 cctgccgctg accgtgttcg gcgtggcgat ggcgctcgaa cgccatccgg aagtcacgac
     1141 cgccttcgtc gaactcggcc acgagatcgc ctgccacggc tggcgctgga tccactacca
     1201 gaacatcgac gaggccaccg agcgcgagca cctgcgcatc gggctggaag tcatcgagca
     1261 cctgaccggc acgcgcccgc tcggctggta caccggccgc gattcgccga acacgcgccg
     1321 gctggtcgtc gatgacggcg gcctgctgta cgactccgac tactacggcg acgacctgcc
     1381 gttctggacc gaggtcacgc gcagcgacgg cgtgaccatg ccgcacctga tcgtgccgta
     1441 cacgctcgat gccaacgaca tgcgcttctc gctgccgcag ggcttttcgc acggcgacga
     1501 gttcttcgag tacctgcgcg acagcttcga cgtgctctac gccgagggcg cggagacgcc
     1561 gaggatgctg tcggtcggcc tgcactgccg gctgatcggc cgccccggac gcttccgtgc
     1621 gctgcagcgt tttctcgatc acatcgagcg ccacgaccgt gtctgggtct gccggcgcat
     1681 cgacatcgcc cggcactggc atgcggagca tccgtacgtg ccgggctgat tcctccggcc
     1741 atccgtctgc tgttcagccc ctcgcccctg gccggagagg ggttggggag agggggaggc
     1801 tgccggcaca gcgtttcagc gaattccccc ggccggcggc ccatc
//
LOCUS       DAOQJH010000621         1844 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_53925_length_1844_cov_6.600894, whole genome shotgun sequence.
ACCESSION   DAOQJH010000621 DAOQJH010000000
VERSION     DAOQJH010000621.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1844)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1844)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1844
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53925_length_1844_cov_6.600894"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            305..1663
                     /locus_tag="PLQ18_12500"
     CDS             305..1663
                     /locus_tag="PLQ18_12500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018452891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding protein"
                     /protein_id="HNE02518.1"
                     /translation="MSKTGNTPRGVDRRDFIRIGLGAAGGLALPAGLAGPARAAVHPP
                     IGTWPAGSSGGTVYAGAAVPLTGSYAEQGADELKGMQLAVEHINEGHPLMKVLSPRTN
                     NGVLGKKLVLLYADSGAKPNEAVQAEQRFISENKVVVMTGSTSSAVAVAMNKLAQREK
                     VLYFAGISGSNDTTGKDCVRYGFRQNFFGHPAAAALGPVMVRQFGKNRKAAFMTPDYT
                     YGHTVTKSVNDYLSQNAGWQMVTNQVSPLGTQDFSQYLINIANSGAEFLINVNWGRDA
                     VLSTQQAHRFGILPKMTLVIPYQIPFLAPQVGADIMQGVYAATDFWWTMGEMPQYPNA
                     KMFVDAFQKKYGYRPEWGAENGYVSFAHWARMVEQAGSFYPPDVIRTWEKGETIPSLV
                     GPVHYRPQDHQCVRPVVIVRGKKPADMRNKEDFWDVLEVVPGEGLMQAPDAFGCQPGD
                     YT"
     gene            1739..>1844
                     /locus_tag="PLQ18_12505"
     CDS             1739..>1844
                     /locus_tag="PLQ18_12505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002718462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNE02519.1"
                     /translation="MITWGTFVSQLFNGLALGALLALISSGLTIIYGAL"
BASE COUNT          315 a          632 c          623 g          274 t
ORIGIN      
        1 gcacgagcat cgcgtggtag tgctgcgcgg tcagcccggc cgcggcggcg gcttcctcgc
       61 tgaagtgcag gaagccccgc agcgcgtgcc ggaagcccgc cagcttcgcg tagtcgcccg
      121 tcgtcagccg gtactgcctc tcggcgcgct tcagttgcac ggcacctccc tccaggccgc
      181 gatcttgcca cacgggcgcc ggaaatccgg taatatcgtg ccccgataca tcttgatgta
      241 tcgtcccgcg atataatacg gaagccgtcg accggctgcg cgggccaact ccaggaggat
      301 cgccatgtcg aagaccggga ataccccgcg cggcgtcgac cgccgcgact tcatccgcat
      361 cgggctgggc gccgcgggcg gcctcgcgct gcccgcggga ctcgccggcc cggcccgtgc
      421 cgccgtccac ccgccgatcg gcacctggcc ggccggctct tccggcggca ccgtgtacgc
      481 cggcgcggcc gtcccgctga ccgggagcta cgcggaacag ggcgcggacg agctcaaggg
      541 catgcagctc gccgtcgagc acatcaacga gggacacccg ctcatgaagg tgctctcgcc
      601 cagaacgaac aacggcgtgc tcggcaagaa gctcgtgctg ctctacgccg actcgggcgc
      661 gaagccgaac gaggccgtgc aggccgagca gcgcttcatc agcgagaaca aggtcgtcgt
      721 gatgaccggg tcgacgtcga gcgcggtcgc cgtcgcgatg aacaagctgg cccagcgcga
      781 gaaggtgctg tacttcgccg ggatctcggg ctcgaacgac accaccggca aggattgcgt
      841 gcgctacgga ttccggcaga acttcttcgg ccatcccgct gcggcggcgc tcggcccggt
      901 gatggtcagg cagttcggca agaaccgcaa ggcggcgttc atgacgccgg actacaccta
      961 cgggcacacg gtgaccaagt cggtcaacga ctacctgagc cagaacgccg gatggcagat
     1021 ggtgaccaac caggtctcgc cgctggggac gcaggacttc agtcagtacc tgatcaacat
     1081 cgccaactcg ggcgccgagt tcctgatcaa cgtgaactgg ggccgcgacg cggtgctgtc
     1141 gacgcagcag gcgcaccgct tcggcatcct gccgaagatg acgctggtga tcccctacca
     1201 gattccgttc ctcgcgccac aggtcggcgc cgacatcatg cagggcgtct acgcggcgac
     1261 ggacttctgg tggacgatgg gcgagatgcc gcagtacccg aacgcgaaga tgttcgtcga
     1321 cgccttccag aagaaatacg gataccggcc ggagtggggc gcggagaacg gctacgtcag
     1381 cttcgcgcac tgggcccgga tggtcgagca ggcgggctcg ttctatccgc cggacgtcat
     1441 caggacctgg gagaagggcg agacgatccc gtcgctcgtc ggcccggtgc actaccggcc
     1501 ccaggatcac cagtgcgttc gccccgtggt catcgttcgc ggcaagaagc ccgcggacat
     1561 gcgcaacaag gaggacttct gggacgtgct cgaggtggtg ccgggcgaag gcctgatgca
     1621 ggcgcccgac gccttcggct gccagccggg cgactacacc tgacgtcgcc gcgcaagccc
     1681 gcatcgacgc cgcgggcgcc gtgacgcccg cggcgccgcg cgagcggggg acgatcgcat
     1741 gatcacctgg ggcacgttcg tttcgcagct gttcaacggt ctcgcgctcg gcgcgctgct
     1801 cgcgctcatc agctcggggc tgacgatcat ctacggcgcg ctcg
//
LOCUS       DAOQJH010000622         1842 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_54023_length_1842_cov_4.549524, whole genome shotgun sequence.
ACCESSION   DAOQJH010000622 DAOQJH010000000
VERSION     DAOQJH010000622.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1842
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54023_length_1842_cov_4.549524"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1434)
                     /locus_tag="PLQ18_12510"
     CDS             complement(<1..1434)
                     /locus_tag="PLQ18_12510"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNE02520.1"
                     /translation="MSLDSTIRLLLVDRPQADAQPVLRPLRAAGFLVRHWQADRIDTL
                     QHLIDEQTFDLAIVRIGEGVLPIENVRDSIDSAFKDIPVIAVADETAGIDAVQLLNAG
                     ADRIAALNHSNSLPLVARLELGRLNERRQARRWEGLYREAEARAQALLETSRDAIAYI
                     HAGAHIYTNPSYRALFGFDDALVDTTLIDLIHRDSRDAVKSYLRARERNPNAPLGDGL
                     ECRGVRADGSEIRIVLLASAARMNEEFCLQLLVREPRGEQPEVQDKLRYFVEHDTLTG
                     LFNRQYFTELLNRIHTSATTSGSLGGAVLYVLMTDYRGVAERLGLEAVDDLLRGVAEA
                     IQQQVGPQEIVARFSDATFTVHTPLSARGAVAELGERVRQGIENHTIQAGGMLITAKA
                     SIGICMIGDTNSSATQIISYADKACEQARQLGGSQVQIYTPVAPERDSIARRDTILRA
                     FRQAVVEQRVHLLRALDVDLRRVAHPVG"
     gene            complement(1489..>1842)
                     /locus_tag="PLQ18_12515"
     CDS             complement(1489..>1842)
                     /locus_tag="PLQ18_12515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011410171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HNE02521.1"
                     /translation="VVLVLHVNHAREIDAALVQACARLRAAGVTLLNQSVLLAGVNDA
                     ATTLAALSEALFAAGVLPYYLHLLDRVGGATHFEVSEQQALSIMGALRAMLPGYLVPR
                     LVREEAGRPAKTPLC"
BASE COUNT          281 a          595 c          677 g          289 t
ORIGIN      
        1 cccgaccgga tgcgctaccc gacggagatc tacgtcaaga gcccggagga gatgcacgcg
       61 ctgttcgacc acggcctgac ggaaggcgcg caggatggtg tcgcgccgcg cgatcgagtc
      121 gcgctccggg gcgaccggcg tgtagatctg cacctgactg ccgccgagct ggcgggcctg
      181 ctcgcaggcc ttgtcggcgt aggaaatgat ctgcgtggcg ctggagttgg tgtcgccgat
      241 catgcagatg ccgatgctgg ccttggcggt gatcagcatg ccgccggcct ggatcgtgtg
      301 gttctcgatg ccctggcgca cgcgctcgcc gagttcggcg acggccccgc gcgccgacag
      361 cggcgtatgc accgtgaagg ttgcatccga gaagcgggca acgatctcct gcggcccgac
      421 ctgctgctgg atcgcctcgg cgacgccgcg cagcaggtcg tcgacggctt cgagcccgag
      481 gcgttcggcg acaccgcggt agtcggtcat cagcacgtag agcacggcac cgccgaggct
      541 gccgctggtg gtcgccgagg tgtggatgcg gttcagcagc tcggtgaagt actggcggtt
      601 gaacagcccg gtcagcgtgt cgtgttcgac gaagtaacgc agcttgtcct gcacctcggg
      661 ctgctcgccg cgcggttcgc gcaccagcag ctgcaggcag aattcctcgt tcatgcgcgc
      721 tgccgaggcg agcagcacga tgcggatctc gctgccgtcg gcgcgcacgc cgcggcattc
      781 gaggccgtcg ccgagcgggg cgttcgggtt gcgctcgcgg gcgcgcaggt agctcttgac
      841 cgcgtcgcgg ctgtcgcggt ggatcaggtc gatcagcgtg gtgtcgacca gcgcgtcgtc
      901 gaaaccgaac agcgcccggt agctcgggtt ggtgtagatg tgcgcgccgg cgtggatgta
      961 ggcgatcgca tcgcgcgagg tttcgagcag cgcctgcgcg cgggcctcgg cctcgcggta
     1021 caggccttcc cagcgccgcg cctggcggcg ttcgttgagc cggccgagtt cgagccgggc
     1081 gaccagcggc agcgagttcg agtgattcag cgccgcgatg cggtcggccc cggcgttgag
     1141 caactgcacg gcgtcgatgc cggcggtctc atcggcgacg gcgatgaccg ggatgtcctt
     1201 gaaggcgctg tcgatcgagt cacggacgtt ctcgatcggc agcacgccct cgccgatgcg
     1261 cacgatggcg aggtcgaagg tctgttcgtc gatcaggtgc tgcagcgtgt cgatgcggtc
     1321 ggcctgccag tggcgcacca ggaaacccgc cgcccgcagc gggcgcagta ccggctgggc
     1381 atcggcctgg ggccggtcga ccagcagcag acggatcgtc gaatcaaggg acacgcgcgg
     1441 tttacctcgg gtcaggacgc gacggatggc gctccgtgca ggctggaatc agcagagggg
     1501 agtcttggcg ggccggccgg cttcctcgcg taccagccgg ggcaccagat aaccgggcag
     1561 catcgcccgc agtgcgccca tgatagacaa ggcctgttgc tcggaaacct caaagtgtgt
     1621 cgcacccccc acgcgatcga gcaggtgcag gtaatacggc agcacgccgg cggcgaacaa
     1681 ggcctcggac agcgcggcca gcgtcgtcgc cgcgtcgttg acgccggcga gcagcaccga
     1741 ctggttcagc agcgtcacgc ccgcggcgcg caggcgtgcg caggcctgca ccagcgcggc
     1801 atcgatctcg cgggcatgat tgacgtgcag cacgagcacg ac
//
LOCUS       DAOQJH010000623         1838 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_54305_length_1838_cov_3.297252, whole genome shotgun sequence.
ACCESSION   DAOQJH010000623 DAOQJH010000000
VERSION     DAOQJH010000623.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1838)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1838)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1838
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54305_length_1838_cov_3.297252"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..508
                     /locus_tag="PLQ18_12520"
     CDS             <1..508
                     /locus_tag="PLQ18_12520"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HNE02522.1"
                     /translation="LESLAKLRRAFRDGGTITAGNAPGVNAAAAAMVVCKRAWADAHG
                     LTPMLVLRASAVVGVEPGLFGYGPVPAIRKVLTRTGWSVGEIDRVEVNEAFAAVALVA
                     QQQLDIDPARFNVDGGAIAHGHPIGATGAVLIVRLAHALARTGGQRGIVSLCIGGGQG
                     IALALERP"
     gene            complement(1114..>1838)
                     /locus_tag="PLQ18_12525"
     CDS             complement(1114..>1838)
                     /locus_tag="PLQ18_12525"
                     /inference="COORDINATES: protein motif:HMM:TIGR03317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="folate-binding protein"
                     /protein_id="HNE02523.1"
                     /translation="ERAAALAQRLKMYVLRAKVELSDVSPQFAALGVSGAGAGARLAG
                     VLGVPVPEQADRALTHDGLTVLGLPAAPPRLAVIGDPARIDALQQALLAAGARAATAE
                     DWATLAIAAGIAEVHGPLVEAFVPQMLNLDRTDGISFSKGCYTGQEIVARVHYLGQIK
                     QRLYRAHVDAPAAPAVAEALIADQAGPNEQAGQIVSVAADPAGGWQMLAVVTTQLAAR
                     DAVRLADGTPLRLQPLHAGQDT"
BASE COUNT          245 a          629 c          678 g          286 t
ORIGIN      
        1 gctcgaatcg ctcgcgaagc tcaggcgggc gttccgtgac ggcggcacga tcaccgccgg
       61 caacgcgccc ggcgtcaatg ccgcggcggc ggcgatggtg gtctgcaagc gcgcctgggc
      121 cgatgcgcac gggctgacgc cgatgctggt gctgcgcgcg agcgctgtgg tcggcgtcga
      181 accgggtctg ttcggctacg ggccggtgcc ggcgatccgg aaggtgctga cgcgcaccgg
      241 ctggtcggtc ggcgagatcg accgcgtcga ggtcaacgaa gccttcgcgg ccgtggcgct
      301 ggtcgcgcag cagcagctcg acatcgatcc ggcgcgcttc aacgtcgatg gcggcgccat
      361 cgcccacggt cacccgatcg gcgccaccgg cgcggtcctg atcgtgcgcc tcgcgcacgc
      421 gctggcgcgc accggcgggc agcgcggcat cgtgtcgctc tgcatcggtg gcggtcaggg
      481 catcgcgctg gcgctggaac ggccgtgagg cgctgccggc ggcggggccc gcagggcggg
      541 ccgtgcgccc ggcaggcctg aatctgcccg accccggggg ccggtccgtg caggtccggc
      601 ccccggtttt catgcggctg ccgcccatgg caactgactc tcgggatgca cggctgcgcc
      661 ggccgggcac ggaagccatc ccggcttccg gtggaattca ccggcgactc cgatacctgt
      721 ttccggaagg aatgtgcagc gcacatcttc cggaaagtgc atgccgtaaa acgccagctt
      781 ccggttccgc ctgccatcgg cgcttgcgga aataccgggc cgtttcagcg gggcggcggc
      841 ggaattacaa gcttaatatt ttgtaagttg atgattatgc cgtcaaacgt ctgatcgcgt
      901 ctgcagatga gggcctgcgg aatcagccgg atttccggtt gccgccatgg tggcgaatga
      961 gcatcgcgcc aggctgccgg ggcggggccg taatcatctt gtccgcagga tttcagcgat
     1021 gtgcgggcgt aatgatgtgc gggaggcctc catgacctcc atggaaccgc ctccgtaatc
     1081 cggcccgggc cggccgcgcg cggaacgtcc gagtcaggtg tcctgtccgg cgtgcagcgg
     1141 ctgcaggcgc agcggcgtgc cgtcggcgag ccggacggcg tcccgggcgg cgagttgtgt
     1201 cgtgacgacg gccagcatct gccagccgcc cgcagggtcg gcggcgacgc tgacgatctg
     1261 gccggcctgc tcgttcgggc cggcctgatc cgcgatcagc gcctcggcca cggccggtgc
     1321 cgcgggggca tcgacgtgcg cccggtacag ccgctgcttg atctggccga ggtaatgcac
     1381 gcgcgcgacg atctcctggc cggtgtagca gcccttgctg aagctgatgc cgtcggtgcg
     1441 gtcgaggttc agcatctgcg gcacgaaggc ttcgaccagc ggaccgtgca cctcggcgat
     1501 gccggcggcg atggcgagcg tcgcccagtc ttcggccgtg gcggcacgcg ctccggctgc
     1561 gagcagcgcc tgctgcaggg cgtcgatgcg ggccggatcg ccgatcacgg ccagacgcgg
     1621 cggcgctgcg ggcaggccga gcacggtgag gccatcgtgc gtcagcgcgc ggtccgcctg
     1681 ctccggcacc ggcacgccca gtaccccggc aagccgtgcg ccggcgcctg caccgctcac
     1741 gccgagggcg gcgaactgcg gactcacgtc gctcagttcc accttcgcgc gcagcacgta
     1801 catcttcagc cgctgtgcca gcgcggccgc gcgctcgg
//
LOCUS       DAOQJH010000624         1827 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_54948_length_1827_cov_2.826185, whole genome shotgun sequence.
ACCESSION   DAOQJH010000624 DAOQJH010000000
VERSION     DAOQJH010000624.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1827)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1827)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1827
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54948_length_1827_cov_2.826185"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1186
                     /locus_tag="PLQ18_12530"
     CDS             <1..1186
                     /locus_tag="PLQ18_12530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02524.1"
                     /translation="LALARSFSLHAAATWLLETRPGPLTLVCYPLLAELSAIFAAYLP
                     PCPAAVPEQDFRRYRGVLPGAYRLADQLLGRLLELGGMDAAVLVVSTHGYLDVPGSRG
                     HGPGLADPRPATPRPEGMWTLSVPGLSAVREAQPATVLDLAPTVLTALGLAPPGDWPG
                     RTLLPPMQRLRPCPPLPAAAPAAPLFGADTDTVMSKPADDGLPDNDALLAALAGHGFD
                     DPLAGTLLAEAAFADSRRGWNRYRIHIQRQDLRGAWQALDALCDGPLAARAALERIAL
                     LGALGRWPEAARRLAPLRPALAGGLAEALAEPWLHARALHLAADAIEAHARGDHWAAR
                     QALNKLEAPLATIPCFLRMQHELDRLPAHASRPEAVPAPKPRLTRGRPKPGAGPGSSD
                     RL"
BASE COUNT          267 a          709 c          600 g          251 t
ORIGIN      
        1 gctggcgctg gcgcgcagct tcagcctgca tgccgcggcg acctggctgc tggagacccg
       61 ccccgggccg ctgacgctcg tctgctatcc gctgctcgcc gaactgtcgg cgatcttcgc
      121 cgcctacctg ccgccgtgtc cggccgcggt gcccgaacag gatttccgcc gctaccgtgg
      181 cgtgctgccc ggtgcctacc ggctcgccga ccagctgctc ggccgcctgc tcgaactcgg
      241 cggcatggac gctgccgtgc tcgtcgtcag cacgcatggc tatctcgacg tgccgggctc
      301 acgggggcac ggccccgggc tcgcggaccc gcgcccggcg acgccgcgcc ccgagggcat
      361 gtggacgctc agcgtgccgg ggctgtccgc cgtgcgcgag gcgcagccgg cgaccgtgct
      421 cgatctggcg ccgaccgtgc tgaccgcgct cggtctggct ccgcccggcg actggccggg
      481 gcgcacgctg ctgccaccga tgcaacgcct gcgtccgtgc ccgccgctgc cggccgccgc
      541 gcccgccgcc ccacttttcg gggccgacac cgataccgtc atgtcaaagc cggcggatga
      601 cggtctgccc gacaacgatg ccctgctcgc ggcgctggcc gggcacggtt tcgacgatcc
      661 gctggccggc acgctgctcg ccgaggccgc gttcgccgac agccgccgcg gctggaaccg
      721 ctaccgcatc cacatccagc gtcaggatct gcgcggcgcc tggcaggcac tcgatgcgct
      781 ctgcgacggg ccgctggccg cacgggcggc actggagcgc atcgcgctgc tcggcgcact
      841 cgggcgctgg ccggaggcgg cgcgccggct ggccccgctg cgcccggcgc tggccggcgg
      901 tctggccgag gcgctggccg agccctggct gcacgcacgc gcgctgcacc ttgccgccga
      961 tgccatcgag gcccacgccc gcggcgatca ctgggcagca cgtcaggcgc tgaacaagct
     1021 cgaagcgccg ctcgcgacca tcccctgctt cctgcgcatg cagcacgaac tcgaccgcct
     1081 gcccgcgcac gccagcaggc ccgaggccgt gccggcaccg aagccgcgcc tgacgcgcgg
     1141 ccgccccaaa ccgggcgcgg gaccgggcag cagcgaccgg ttgtaagcgg gccgcagccg
     1201 ggcgccgtcc ccgcatcgca gcacgagccc cgtgcatcgg catgtacccg agtcaaagat
     1261 tttcttgcca atcaacgaaa tgccgattcg catcagaatc ggcaagcgat tgatgtacaa
     1321 gaacatctca aaataagggc agatacatgc cgatgccgga cggccggccc ggaggccccg
     1381 agccgcaccg cgcctctcct caccggcccg gctgcgcagc cggccctgtc cgccttcacg
     1441 ctgccgcccg gcggtcccga tttcgcggct gcgcaactcg ggcaacagtt ccggatcaac
     1501 ggcgccggca tcgccactcg cgtgacgatc tcctgccggc gcgatacccg tggccccctg
     1561 gcgatcgagc ggaccatcga tgctggacgc atggacccgt ccggattgag cccccagacc
     1621 cgctgcgccc gccgacagca tcagttcgtc cagaaagaaa cccgaaaccg tccggaacgg
     1681 cgtgccggac aaccaccccg acggcggggc tttgcactcc agccggacac ttgagagacg
     1741 gggccaataa aaaaggcttg cgcttcaagc acaagccttt gatttcatgg taggccctgc
     1801 aggacttgaa cctgcgacca agcgatt
//
LOCUS       DAOQJH010000625         1826 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_55014_length_1826_cov_2.939582, whole genome shotgun sequence.
ACCESSION   DAOQJH010000625 DAOQJH010000000
VERSION     DAOQJH010000625.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1826
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55014_length_1826_cov_2.939582"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1826)
                     /locus_tag="PLQ18_12535"
     CDS             complement(<1..>1826)
                     /locus_tag="PLQ18_12535"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNE02525.1"
                     /translation="AVSGLPGESSARLSAWVRTGLTYFAAQCYDALPAPVTLLDASPG
                     DWPQLCTAVPEAVATAADALARLARAYRLRPAELFLIALAGEIEADFRVVLAIAELQA
                     PERTARPALHLACALTETLFPQQKRLTPLTLAGCAAVRAGLLAIDGDGPLPMRSLRLD
                     ARLWATLGGDDTVWPDTQWLTPADAELPAATRARLDPLARALADGDFDVLVLRGMPYS
                     GRGRCAAALAARLGLQACAITSERWAADAALAAACHCCGWLPVLRPRLAPGERYEPPR
                     HPGYDGALIVLLGEAGAVAGTRVFEETLPAATAGERAASWAAAGIPDPPATVLGGPAI
                     AALLRSAAWQAGADPAGRLLAARRQLAAPALRQLAQAVTPVAREALVLTASTAVELDA
                     FIARCERRESAWRGLGPSAQAGAGAGVRALFAGESGTGKTLAAAVVATALGAPLYRVD
                     IGALMNKYIGETEKNLAALLDHAAGTDAMLLFDEADALFGRRSDGGDTGERFANLLTN
                     YLLTRIESHPGLVVLTSNHRARIDAAFTRRLDAIVEFPLPGPEERLALWLALLGERTP
                     PASELQHLASYAELAGGHIRNAVIAAAAQQPDGPLPVPLLAR"
BASE COUNT          233 a          663 c          701 g          229 t
ORIGIN      
        1 ccgcgccagc agcggaaccg gcagcggtcc gtccggctgc tgcgccgcgg cggcgatcac
       61 ggcgttgcgg atgtggccgc cggcgagttc ggcatagctt gcaaggtgct ggagttcact
      121 cgccggcggt gtgcgttcgc cgagcaatgc gagccacagc gccaaccgtt cttcgggacc
      181 gggcagcggg aactcgacga tggcatcgag ccggcgcgtg aaggccgcgt cgatgcgggc
      241 gcggtggttg ctggtcagca cgaccagacc gggatggctc tcgatgcgcg tcagcaggta
      301 gttggtcagc aggttcgcga agcgttcccc ggtgtcgccg ccgtcgctgc gccggccgaa
      361 cagcgcgtcg gcctcgtcga acagcagcat tgcatcggtg ccggcggcgt gatcgagcag
      421 cgcggcgagg ttcttctcgg tttcgccgat gtacttgttc atcagcgcgc cgatgtcgac
      481 ccggtacagc ggcgcgccga gcgccgttgc gaccacggct gcggccagcg tcttgccggt
      541 gccggattcg ccggcgaaca gcgcccgcac gccggcgccg gcgccggcct gcgcgctcgg
      601 gccgagtcct cgccatgcgc tttcccgccg ttcgcagcgc gcgatgaagg cgtcgagttc
      661 gactgcggtc gaggccgtca gcaccagggc ctcgcgcgcg accggcgtga ccgcctgcgc
      721 gagctggcgc agcgccgggg ccgcgagctg gcgccgggcg gcgagcaggc gtccggccgg
      781 atccgcaccg gcctgccagg cggccgagcg cagcagggct gcgatcgccg gaccgccgag
      841 cacggtcgcc ggcggatcgg ggatgccggc tgcggcccag ctcgccgcgc gctcgccggc
      901 ggtcgccgcc ggcagggttt cctcgaacac ccgggtgccg gcgaccgctc cggcctcgcc
      961 gagcagcacg atcagcgctc cgtcgtagcc cggatgccgg gggggttcat agcgttcgcc
     1021 gggcgccagc cgcggtcgca gcaccggcag ccagccgcag cagtggcagg ccgccgccag
     1081 cgcggcatct gcggcccatc gttcggacgt gatcgcgcag gcctgcagcc cgagccgggc
     1141 ggccagcgcg gcggcgcagc gtccgcggcc gctgtacggc atgccgcgca gcaccagcac
     1201 gtcgaaatct ccgtccgcga gcgcgcgggc gagcggatcg agccgggcgc gggtcgcggc
     1261 cggcagttcg gcatcggccg gcgtcagcca ctgcgtatcc ggccagaccg tgtcgtcccc
     1321 gccgagcgtg gcccacagcc gcgcatcgag ccgcaggctg cgcatcggca gcggaccgtc
     1381 gccgtcgatc gcgagcagtc cggcgcgtac cgcggcgcag ccggcgagcg tgagcggcgt
     1441 caggcgtttc tgctgcggaa acagggtttc ggtcagcgcg caggccagat gcagcgccgg
     1501 ccgcgccgtg cgctccggcg cctgcagttc ggcgatcgcc agcacgacgc ggaaatcggc
     1561 ctcgatctcg ccggcgagcg cgatcaggaa cagctcggcc gggcgcaagc gatacgcgcg
     1621 cgccagccgc gccagcgcgt ctgctgcggt ggcgacggcc tcgggcacgg cggtgcagag
     1681 ctgcggccag tccccggggc tcgcgtcgag cagcgtgacc ggcgcgggca gcgcgtcgta
     1741 gcactgcgcg gcgaagtatg tgagtccggt gcgcacccag gccgacagcc gcgccgatga
     1801 ttccccgggc agcccggaca cggccg
//
LOCUS       DAOQJH010000626         1815 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_55650_length_1815_cov_3.341477, whole genome shotgun sequence.
ACCESSION   DAOQJH010000626 DAOQJH010000000
VERSION     DAOQJH010000626.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1815)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1815)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1815
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55650_length_1815_cov_3.341477"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1070)
                     /locus_tag="PLQ18_12540"
     CDS             complement(<1..1070)
                     /locus_tag="PLQ18_12540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02526.1"
                     /translation="MNKGKKRSRALLAGLLLAPGVAAAIGVGPIQVQSGLGQRLRAQV
                     ELLSVAPDELQNLSVRLAPPQAFAQAGLDRTGALSDLQFAVEAQPGGRSVIRITSQRP
                     VREPALNFLVELGSPRGKLMREFAILLDPVRPNERRATAASPIGGYAGQPSAGGGTYG
                     PIRPGETLWSIADRLRPADMSNREAVRRLYDANPEAFSRRSPDGLRAGAMLRMPAESG
                     WQGNPPAPAVPAPAQPLPVTPPAVATRPPASPAPATAKPPEVPPAGAQVRLLPPEGQG
                     GGNALPAPGMGMTDQPRGPDPVAGGGYGQIELDNGRLRLQAAGLDHLQNRIDTLPPAE
                     RMPVPVTPPVAEPPRPASVTPT"
     gene            complement(1185..>1815)
                     /locus_tag="PLQ18_12545"
     CDS             complement(1185..>1815)
                     /locus_tag="PLQ18_12545"
                     /inference="COORDINATES: protein motif:HMM:NF014793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Asd/ArgC dimerization domain-containing protein"
                     /protein_id="HNE02527.1"
                     /translation="LVLPDAVTLALARVLMPLAALAGIGHVGLTVLRAASDDGRAAVE
                     ELAQQTIALLNARPSNPQIYPRRLAFNVLPEWQDLDAQGRGGGERRLDAGLEQVLGLA
                     PGSVATTLLRVPLFYGLACVVHLRCTAALSPAAARERLLEVPGLEVLEADEAPVSPAE
                     DGLGQPAVRVGGLRAAGERGLNLWVVADNVALPASNGVQIAQTLIGTAE"
BASE COUNT          253 a          635 c          664 g          263 t
ORIGIN      
        1 tgcgttggcg tcacggatgc cggtctgggg ggctcggcaa ccggtggcgt caccggcacc
       61 ggcatacgct cggccggggg cagcgtgtcg atgcggttct gcagatggtc gaggccggcg
      121 gcctgcagcc gcagacggcc gttgtcgagt tcgatctggc cataaccgcc gccggcgacc
      181 gggtccggtc cgcgcggctg gtcggtcatg cccatgcccg gtgcgggcag ggcattgccg
      241 ccgccctgac cttccggcgg cagcagccgg acctgtgcgc cggccggcgg cacctcgggc
      301 ggctttgccg tggcgggcgc cgggcttgcc ggcggcctgg tcgcgacagc cggcggggtc
      361 accggcagcg gctgggccgg agccgggact gccggggccg gcggattgcc ctgccagccg
      421 ctttcggccg gcatgcgcag catggcaccg gcgcgcagcc cgtccggcga gcgccgcgag
      481 aacgcctcgg gattggcatc gtacagacgg cgcacggcct cgcggttcga catgtcggcc
      541 gggcgcaggc gatcggcgat gctccagagg gtttcgcccg ggcggatcgg cccgtacgtg
      601 ccgccgccgg ccgatggctg gcctgcatat ccaccgatcg ggctggcggc ggttgcgcgc
      661 cgctcgttcg ggcgcaccgg atcgagcagg attgcgaact cgcgcatcag cttgccgcgc
      721 ggactgccga gttcgaccag aaagttcagg gccggctcgc gtaccggacg ctgcgaggtg
      781 atgcggatga cgctgcgtcc gcccggctgg gcctcgacgg cgaactgcag gtcggacagc
      841 gccccggtgc gatcgagccc ggcctgcgcg aaggcctgcg gcggcgcgag acggacgctc
      901 aggttctgca gttcgtcggg cgcgaccgac agcagctcga cctgggccct gaggcgttgg
      961 cccagtccgg actggacctg gatcggaccg acgccgatgg cggcggcaac acccggtgcc
     1021 aacagcagtc cggcgagaag ggctcgactg cgttttttcc ctttattcat tgtcggtcgg
     1081 gtctcggcgt ccgtcgcgcg ggatcaaaaa gaccgccggc acgaagggtc cggcggtctg
     1141 cggatcccat tgattataaa ggcgatcatg aatcgccccg tgcgtcattc tgctgttccg
     1201 atcagcgttt gggcgatttg gacgccattg gacgcgggca gggccacatt atccgccact
     1261 acccacagat tcaagccccg ttccccggcg gcccgcagtc cgccgacccg cacggccggc
     1321 tgtccgagac catcctcggc cggtgaaacc ggcgcttcat cggcctccag cacttcgagg
     1381 ccgggcacct ccagcagccg ctcccgggcc gcggcggggg acagcgcggc cgtgcagcgc
     1441 agatgcacca cacaggccag accgtagaac agcggcacgc gcagcagcgt cgtcgcgacg
     1501 ctgccgggcg cgagaccgag cacctgttcg agtccggcat cgagccggcg ctcgccgcca
     1561 ccgcggcctt gcgcatcgag atcctgccat tcgggcagca cgttgaatgc gagccggcgc
     1621 ggatagatct gcggattcga gggccgggcg ttcagcagcg cgatggtctg ctgcgcgagt
     1681 tcctcgaccg cggcgcgacc gtcatccgat gccgccctga gcacggtcag gcccacgtgc
     1741 ccgatgccgg ccagggccgc gagcggcatc agcacccgcg cgagtgcgag cgtcacggca
     1801 tcgggcagca ccagc
//
LOCUS       DAOQJH010000627         1813 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_55781_length_1813_cov_3.336177, whole genome shotgun sequence.
ACCESSION   DAOQJH010000627 DAOQJH010000000
VERSION     DAOQJH010000627.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1813)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1813)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1813
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55781_length_1813_cov_3.336177"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /locus_tag="PLQ18_12550"
     CDS             <1..244
                     /locus_tag="PLQ18_12550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HNE02528.1"
                     /translation="KRPVMSDLRESGAIEQDADLICFIYRDEVYHPDSADKGTAEIII
                     GKQRNGPIGSVRLTFLGKYTRFENFMPDVYAADEYA"
     gene            complement(249..572)
                     /locus_tag="PLQ18_12555"
     CDS             complement(249..572)
                     /locus_tag="PLQ18_12555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02529.1"
                     /translation="MPLTSRNRRSIRSLGILTLVLFVVALTVSIGPAWLINPYIYDTA
                     QISFMSIPLWIATVGLALWVNQMTLDRRRPLDLLTLTLGRAPLLAIPIVLALLLVLTI
                     AGVRL"
     gene            969..>1813
                     /locus_tag="PLQ18_12560"
     CDS             969..>1813
                     /locus_tag="PLQ18_12560"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015281482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HNE02530.1"
                     /translation="MQVGTTVTQFIIEEQRRHAHATGDFTSLINDIVIACKAIANAVS
                     RGSLAGVLGHAGTENVQGEDQKKLDVLANEAFLRCNEWGGRVAAMASEEMDDVYNLPA
                     VYPRGRYLLLFDPLDGSSNTDVNGIIGTIFSVLRCPDGVTEPSAEHFLQPGSQQVAAG
                     MAVYGPQTILVLTTGYGVNGFTLDDTIGEFVLTHPEMRVPEDSAEFAINASNQRHWEP
                     PVQRYIDECLAGSDGPRGRDFNMRWMGAMVGDVYRVLCRGGVFLYPADAKTAKKGGKL
                     RLMYEA"
BASE COUNT          319 a          593 c          629 g          272 t
ORIGIN      
        1 caagcggccg gtgatgtcgg atctgcgcga atcgggcgcg atcgagcagg atgccgacct
       61 gatctgcttc atctatcgcg acgaggtcta tcacccggac agtgccgaca agggcacggc
      121 cgagatcatc atcggcaagc agcgcaatgg tccgatcggt tcggtgcgcc tgaccttcct
      181 cggcaagtac acgcgcttcg agaacttcat gcccgacgtg tacgcggcgg acgagtacgc
      241 ctgaagcctc agagccgcac gccggcgatc gtcagcacca gcagcagtgc cagcacgatc
      301 ggaatcgcca gcagcggcgc ccgcccgagc gtcagcgtca gcaggtcgag cggccggcgg
      361 cggtccagcg tcatctggtt gacccacagc gccagcccga cggtcgcgat ccacagcggg
      421 atgctcatga acgagatctg cgccgtgtcg tagatgtacg ggttgatcag ccaggccggc
      481 ccgatcgaga cggtcagggc gacgacgaac agcacgaggg tcaggatgcc gagcgagcgg
      541 atcgagcgtc ggttgcgtga agtgaggggc atggcggcgg taccagatgc ggcagcggtg
      601 ggcacggggg gtcgtgccag atgcagttcc gggctccctg ccggaggtca tgcggcgagt
      661 gtgggacaga acgccggcgg cgggaagtca cggtttgcgg cacaacgtga cgttcgcgca
      721 atgaagcggc acaaaatcgc aacgatggcc ggcgatcgcg catccgggcc atgccggcgg
      781 tccgtgtccc ggcgccggtt ccgggccgtg ccgcatcccg gtggtcccgg gcggcggggc
      841 gcagaccttg tggtaaagtt ttcagtcccg ccgtcggccc gggggcaggg tgcccgcgtc
      901 gcataccgtg ctcctggtct ccggaccgga actcttccgg cagcggcacc gtgtcgaagg
      961 aatcccccat gcaagtcgga accaccgtca cgcagttcat catcgaggag cagcgccggc
     1021 acgcccatgc gaccggcgat ttcacctcgc tgatcaacga tatcgtcatc gcctgcaagg
     1081 cgatcgcgaa cgccgtcagc cgcggatcgc tcgccggcgt gctcggccac gccggcaccg
     1141 agaacgtgca gggcgaggac cagaagaagc tcgacgtgct cgccaacgaa gccttcctgc
     1201 gctgcaacga atggggcggg cgcgtggcgg cgatggcgtc ggaggaaatg gacgacgtct
     1261 acaacctgcc ggccgtgtat ccgcgcggcc gctacctgct gctgttcgat ccgctcgacg
     1321 gctcctcgaa caccgacgtg aacggcatca tcggcacgat cttctcggtg ctgcgctgcc
     1381 cggacggcgt caccgagccg agcgccgagc acttcctgca gcccggctcg cagcaggtcg
     1441 ccgccggcat ggcggtctat ggtccgcaga cgatcctcgt gctgaccacc ggctatggcg
     1501 tcaacggctt cacgctcgat gacacgatcg gcgagttcgt gctgacgcat cccgagatgc
     1561 gcgtgcccga ggacagcgcc gagttcgcga tcaacgcctc gaatcagcgc cactgggagc
     1621 cgccggtgca gcgctacatc gacgaatgtc tggccggcag cgacggcccg cgcggacgcg
     1681 acttcaacat gcgctggatg ggggcgatgg tcggcgatgt ctaccgcgtg ctctgccgcg
     1741 gcggcgtgtt cctgtacccg gccgacgcca agaccgcgaa gaagggcggc aagctgcggc
     1801 tgatgtacga ggc
//
LOCUS       DAOQJH010000628         1810 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_55967_length_1810_cov_2.739601, whole genome shotgun sequence.
ACCESSION   DAOQJH010000628 DAOQJH010000000
VERSION     DAOQJH010000628.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1810
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55967_length_1810_cov_2.739601"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..477)
                     /locus_tag="PLQ18_12565"
     CDS             complement(<1..477)
                     /locus_tag="PLQ18_12565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein"
                     /protein_id="HNE02531.1"
                     /translation="MTRKLFWDDPYRSALDTVVASVDGETVRLAETIFYAFSGGQESD
                     HGCIGGLPVLEARRQGHDIAYTLPAGHGLQGGMPVTVEIDWPRRYRLMRLHFAAELVL
                     ELVCARCPGIDRIGAHIGADKARIDFAWPQPLTPLLPDLHGQVSALIEADRPIISAF"
     gene            complement(488..1402)
                     /locus_tag="PLQ18_12570"
     CDS             complement(488..1402)
                     /locus_tag="PLQ18_12570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HNE02532.1"
                     /translation="MKTRSSAASRPPLPARDGVDPCSLRLPAGDWKELLGFLQERFPD
                     DSYVPWTARLARGEVVSEDGETLTTTSTYRPGLRIWYYREPGPEAALPYVETVLHRDA
                     HLLVVDKPHFLPVVPGGPWLRETLLVRLRVRLGLPGLTPLHRLDRLTAGLVLFSVDPA
                     TRAAYAALFPERRITKTYEALAPALPEVPAVRRSRLVAGEPFFRMCEVAGPPNTETRI
                     GLIERRDALGRYRLQPLTGRKHQLRVHMAALGAPVLNDPLYPQLQAGASDDPARPLAL
                     LARELRFTDPLSGEPRCYHSRLHLDFPA"
     gene            complement(1500..>1810)
                     /locus_tag="PLQ18_12575"
     CDS             complement(1500..>1810)
                     /locus_tag="PLQ18_12575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018715709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HNE02533.1"
                     /translation="LGTDSAPHARHTKEAACGCAGCYTAPFALELYAEAFESAGALDR
                     LDAFASRFGPAFYGLPVNAGTVTLEKTAVEVPAGYPYPEGETLVPLRAGERLAWRQIA
                     "
BASE COUNT          265 a          627 c          670 g          248 t
ORIGIN      
        1 gaaggcgctg atgatcggcc ggtcggcttc gatcagcgcg ctgacctgcc cgtggaggtc
       61 cggcagcagc ggcgtcagcg gctgcggcca cgcgaagtcg atgcgtgcct tgtccgcgcc
      121 gatgtgcgcg ccgatccggt cgatgcccgg gcagcgcgcg cagacgagtt ccagcaccag
      181 ttcggccgcg aagtgcagcc gcatcagccg gtagcgccgc ggccagtcga tctcgaccgt
      241 gaccggcatg ccgccctgca ggccatgtcc ggccggcagc gtgtaggcga tgtcgtgccc
      301 ctgcctgcgg gcttcgagca ccggcaggcc gccgatgcag ccgtggtcgc tctcctgtcc
      361 gcccgagaac gcatagaaga tggtttcggc gagcctcacg gtttcgccgt cgacgctcgc
      421 gaccacggta tcgagcgcgc tgcggtacgg atcgtcccag aacagcttgc gggtcatggc
      481 ggtggtctca ggcaggaaag tccaggtgca ggcggctgtg atagcagcgc ggctcgccgc
      541 tcagcggatc ggtgaagcgc agctcgcgcg ccagcagcgc cagcggccgg gccggatcgt
      601 cggacgcacc ggcctgcagt tgcgggtaga gcggatcgtt caggaccggc gcgccgagcg
      661 cggccatgtg cacgcgcagc tggtgcttgc ggccggtgag cggctgcagc cggtagcggc
      721 ccagggcatc acgtcgctcg atcagcccga tgcgcgtctc ggtgttcggc ggaccggcga
      781 cctcgcacat ccggaaaaac ggctcgccgg cgaccaggcg gctgcggcgc acagcgggca
      841 cttccggcaa ggccggggcc agcgcctcgt aggtcttggt gatgcgccgt tcgggaaaca
      901 gcgccgcata ggccgcgcgc gtggccggat cgaccgagaa cagcaccagc ccggcggtca
      961 gccggtcgag ccggtgcagc ggcgtcagcc ccggcagccc gagccgcacg cgcaggcgca
     1021 ccagcagcgt ctcgcgcagc cacggcccgc cgggcacgac cggcaggaag tgcggcttgt
     1081 cgacgaccag caggtgcgca tcccggtgca gcacggtctc gacgtacggc agcgctgctt
     1141 ccggccccgg ctcgcggtag taccagatgc gcagccccgg gcggtaggtg ctggtcgtcg
     1201 tcagggtttc gccgtcctcg ctgacgacct cgccgcgcgc cagccgtgct gtccagggca
     1261 cgtagctgtc atcgggaaag cgttcctgca ggaagccgag cagctccttc cagtcgccgg
     1321 ccggcaggcg cagcgagcag ggatcgacgc catcgcgcgc aggcagcggt ggccgcgacg
     1381 cggcggatga gcgggttttc atggatgcca gcgttcacgg gagcgggcag cggcatccgg
     1441 ggagccgggg gccggccacc ggtaccgacg atccggacgg ccggggcggg gcgcaggatt
     1501 caggcgatct gacgccacgc gagtctttcc cccgcccgca gcggcaccag cgtctcgcct
     1561 tccggatacg ggtagcccgc cggcacctcc acggctgtct tctccagcgt caccgtcccg
     1621 gcattgaccg gcagtccgta gaacgccggt ccgaagcggc tcgcgaacgc atcgagccga
     1681 tcgagcgcgc cggcggactc gaaggcttcc gcatagagtt ccagcgcgaa cggcgccgta
     1741 tagcagccgg cgcaaccgca ggcggcttcc ttggtgtggc gggcgtgcgg ggcgctgtcg
     1801 gtgccgagga
//
LOCUS       DAOQJH010000629         1809 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_56020_length_1809_cov_2.870011, whole genome shotgun sequence.
ACCESSION   DAOQJH010000629 DAOQJH010000000
VERSION     DAOQJH010000629.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1809
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56020_length_1809_cov_2.870011"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..690
                     /locus_tag="PLQ18_12580"
     CDS             <1..690
                     /locus_tag="PLQ18_12580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004338583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNE02534.1"
                     /translation="DKREFKDLDDTVELGTRNLKVALRKLRQFARSGAADTLDLDDTI
                     ESTARNAGWLDLKLVPERHNAVKVLLLLDVGGSMDWHVRICEELFSAARAEFKHLKHY
                     YFHNCVYESVWIDNRRRRTERVPTAELLNTYGPDWRLVIVGDATMSPYEITQVNASVE
                     HVNEEPGAVWLQRLFAAWPRAVWLNPQPETAWEQTMSLRLIQRLARQRMYPLTLAGID
                     RAIRSLRSGEA"
     gene            930..>1809
                     /locus_tag="PLQ18_12585"
     CDS             930..>1809
                     /locus_tag="PLQ18_12585"
                     /inference="COORDINATES: protein motif:HMM:NF017085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HNE02535.1"
                     /translation="MSDPARSAVKAEAVRLDPSSDARGALAVIAAAAARQIAANRSLL
                     VDGYDAEAVHQLRVGARRLRAALKLFGRYEPAVRRPPFLPALRTLVAAAGAARDPDVL
                     LDATLPRLAGGSDDPGAVAELARHYAGERDRAHRQLLEACADPASAALLIAIDGWISR
                     LPALGSRVLAEPVTGFAPRLLDRCARRLRHRGRHLLHLDAEARHAVRIAAKELRYAGD
                     GCASLYEAAAAEDWLHALAQLQDTLGELNDLAVLDARLRDAAPAGHGSAAARVRALAA
                     AAARELREPLRRHWRHW"
BASE COUNT          262 a          663 c          635 g          249 t
ORIGIN      
        1 gacaagcgcg agttcaagga tctcgacgac acggtcgaac tcggcacgcg caacctcaag
       61 gtggcgctgc gcaagctgcg ccagttcgcg cgcagcggcg ccgccgacac gctcgatctc
      121 gacgacacca tcgaatcgac tgcgcgcaac gccggctggc tggacctgaa gctggtgccg
      181 gagcgccaca acgccgtcaa ggtgctgctg ctgctcgatg tcggcggctc gatggactgg
      241 cacgtgcgca tctgcgagga gctgttctcg gcggcgcgcg ccgagttcaa gcacctgaag
      301 cattactact tccacaactg cgtctacgag agcgtctgga tcgacaaccg ccgccggcgc
      361 accgagcgcg tgccgacggc cgagctgctg aacacctacg ggccggactg gcggctggtg
      421 atcgtcggcg atgcgacgat gagcccctac gagatcacgc aggtcaacgc cagcgtcgag
      481 cacgtcaacg aggagcccgg tgccgtctgg ctgcagcggc tgttcgcggc ctggccgcgc
      541 gcggtgtggc tgaacccgca gccggaaacg gcctgggaac agacgatgtc gctgcggctg
      601 atccagcgcc tcgcccgcca gcgcatgtac ccgctgacgc tggccgggat cgatcgcgcg
      661 atccgctcgc tgcgcagcgg cgaggcctga tcggcatggg cggtatcagg aaaccggcaa
      721 aaaagaaccc gtccggagtg cgccccggac gggtgacgag ttgcgacttc tcgaatgcgg
      781 tctgatcgac ctgtggctgc cggatccctg agcgccgctg ccgtgtccgt tcggcaaagg
      841 ggctcgatca cgagttccgg gagcctagcg cggtaccggg cgcgcgccag tgcccggccg
      901 ctaaatccac aagttctcac aggaatttaa tgtccgaccc cgcccgcagc gccgtcaagg
      961 ccgaagccgt ccggctcgat ccgtccagcg atgcccgcgg cgcgctcgcc gtgatcgccg
     1021 ccgccgccgc ccgccagatc gcggccaatc gcagcctgct cgtcgacggc tacgacgccg
     1081 aagccgtgca tcagttgcgc gtcggcgccc gccgcctgcg ggcggccctg aagctgttcg
     1141 gccgctacga gcccgccgtg cgccgcccgc cgttcctgcc ggcgctgcgc acgctggtgg
     1201 ccgccgccgg tgcggcgcgc gatcccgatg tgctgctgga tgcgacgctg ccgcggctgg
     1261 ccggcggcag cgacgatccg ggcgcggtcg ccgaactcgc gcgccactac gccggcgagc
     1321 gtgaccgcgc acaccggcag ctgctcgaag cctgcgccga tccggcctct gcggcgctgc
     1381 tgatcgcgat cgacggctgg atcagccggc tgccggcact cgggagccgg gtcctggccg
     1441 agccggtcac cggctttgca ccgcggctgc tcgatcgctg cgcccggcgg ctgcgccacc
     1501 gcggccgtca cctgctgcac ctcgatgccg aagcccgcca cgccgtgcgc atcgccgcca
     1561 aggaactgcg ctacgccggc gacggctgcg cgtcgctgta cgaagcggcg gcggcagagg
     1621 actggctgca tgcgctcgcg cagctgcagg acacgctcgg cgagctcaac gaccttgccg
     1681 tgctcgatgc gcgcctgcgc gacgccgctc cggccggcca cggcagcgcg gccgcccgcg
     1741 tgcgggccct ggcggcggcg gcggctcgcg agttgcggga gccgctgcgc aggcactggc
     1801 ggcactggc
//
LOCUS       DAOQJH010000630         1808 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_56055_length_1808_cov_3.774102, whole genome shotgun sequence.
ACCESSION   DAOQJH010000630 DAOQJH010000000
VERSION     DAOQJH010000630.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1808
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56055_length_1808_cov_3.774102"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..813)
                     /gene="hrcA"
                     /locus_tag="PLQ18_12590"
     CDS             complement(<1..813)
                     /gene="hrcA"
                     /locus_tag="PLQ18_12590"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HNE02536.1"
                     /translation="MARQPVPASQLSDRAQLLLKALVQRYIEDGQPVGSRTLAKNTGM
                     DLSPATIRNVMSDLEELGFLRAPHTSAGRVPTVRGYRFFVDTLMQVRPLENAAVQSME
                     RELRGAHSTHGLVQSASTLLSGLSHMAGIVMIPRRDSVTLRQIEFLPLSERRVLAIIV
                     VNEREVQNRILHVSRDYTRTELTEAANYLNRLFAGHDLQQVRAQLLAELQAAELEAND
                     LLQRVAEMAEQALSPEARELPDDFVLAGQTNLMGFSELADVQRLRGLFEAFTH"
     gene            963..>1808
                     /locus_tag="PLQ18_12595"
     CDS             963..>1808
                     /locus_tag="PLQ18_12595"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006786075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HNE02537.1"
                     /translation="MPYLFSTIGLIGKYNDASVAPTLDQIAGWLRQAGRRVLLDEQSA
                     AHVTVRGPEVATRRLIGRQADLAIVVGGDGTLLDAARALVDFQVPIVGVNLGRLGFLT
                     DVSPNEIPAGLDEILQGRFREELRLMLHAQVLRGEQTLSEAMALNDVVVHKRDVARMV
                     ELDTFLDGQLLNAYRSDGLIISTPTGSTAYALSCGGPILHPALEALLVAPICPHTLTH
                     RPIVVGADAQIEICVRNTTHTQITCDGQISLPIEPGDRVVVRQKRRRMRLLHPVGHDY
                     FELLRA"
BASE COUNT          270 a          640 c          634 g          264 t
ORIGIN      
        1 gtgcgtgaag gcctcgaaca ggccgcgcag gcgctgcacg tcggccagct ccgagaagcc
       61 catcaggttg gtctggccgg cgagcacgaa gtcatcgggc agctcgcgcg cctccgggct
      121 cagcgcctgc tcggccatct cggcgacacg ctgcagcagg tcgttggctt ccagctccgc
      181 ggcctgcagc tcggcgagca gctgcgcgcg cacctgctgc aggtcgtggc cggcgaacag
      241 gcggttcagg tagttcgcgg cctcggtcag ctcggtgcgc gtgtagtcgc gcgagacgtg
      301 caggatgcgg ttctgcacct cacgctcgtt gacgacgatg atcgccagca cgcgccgctc
      361 cgacagcggc aggaactcga tctggcgcag cgtcaccgag tcacggcgcg ggatcatcac
      421 gatgccggcc atgtgcgaga gccccgacag cagcgtcgag gccgactgca cgaggccgtg
      481 cgtgctgtgc gcgccgcgca gctcgcgctc catcgactgc acggcggcgt tctcgagcgg
      541 gcgcacctgc atcagcgtgt cgacgaagaa gcggtagccg cgcacggtcg gcacgcgccc
      601 ggccgaggtg tgcggcgcgc gcaggaagcc gagttcctcg aggtccgaca tgacgttgcg
      661 gatcgtcgcc ggcgacaggt ccatgccggt gttcttggcc agcgtgcgcg agccgaccgg
      721 ctggccgtcc tcgatgtagc gctgcaccag cgccttcagc agcagctggg cgcgatcgga
      781 caactggctg gcgggtacgg gctgacgggc cacggtgcgt ctccggcggc gattggcact
      841 cagggccgta gagtgccaaa cagggtcagc taatcagccc cggcgcgggg tgtcaaggcg
      901 ataggtcggc gcaccgggtg cgtgcgatca tagcccgcac gcgcttccgg aaggcccgca
      961 cgatgcccta tctcttcagc accatcggcc tcatcggcaa gtacaacgac gcgagcgtcg
     1021 cgccgacgct cgaccagatc gccggctggc tcaggcaggc cggccgtcgc gtgctgctcg
     1081 acgagcagag tgccgcgcac gtgacggtgc gcggccccga ggtcgccacg cgccggctga
     1141 tcggccggca ggcggatctc gccatcgtcg tcggcggcga cggcacgctg ctcgacgcgg
     1201 cgcgtgcgct cgtcgacttc caggtgccga tcgtcggcgt caacctcggc cggctcggct
     1261 tcctgaccga cgtgtcgccg aacgagattc cggccggact cgacgagatc ctgcaggggc
     1321 gcttccgcga ggagctgcgc ctgatgctgc acgcgcaggt gctgcgcggc gagcagacgc
     1381 tcagcgaggc gatggcgctc aacgatgtcg tcgtgcacaa gcgcgacgtg gcgcgcatgg
     1441 tcgagctcga caccttcctc gacggccagc tgctgaacgc ctaccgctcc gacggcctga
     1501 tcatctcgac gccgaccggc tcgaccgcgt atgcgctgtc ctgcggcggc ccgatcctgc
     1561 acccggcgct cgaagcgctg ctggtcgcac cgatctgccc gcacacgctg acgcaccgcc
     1621 cgatcgtggt cggggccgac gcgcagatcg agatctgcgt gcgcaacacc acgcacacgc
     1681 agatcacctg cgacggccag atcagcctgc cgatcgagcc gggcgaccgc gtcgtcgtgc
     1741 gccagaagcg ccggcgcatg cgcctgctgc acccggtcgg tcacgactac ttcgagctgc
     1801 tgcgcgcc
//
LOCUS       DAOQJH010000631         1806 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_56189_length_1806_cov_3.763564, whole genome shotgun sequence.
ACCESSION   DAOQJH010000631 DAOQJH010000000
VERSION     DAOQJH010000631.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1806)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1806)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1806
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56189_length_1806_cov_3.763564"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(129..938)
                     /gene="cysQ"
                     /locus_tag="PLQ18_12600"
     CDS             complement(129..938)
                     /gene="cysQ"
                     /locus_tag="PLQ18_12600"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013033233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HNE02538.1"
                     /translation="MSQDFAQWLTPVRAIAAEAAARILEIYDTNFDVETKGDDSPLTA
                     ADRAAHECIVAGLTALTPDIPVLSEESRALPFEQRAAWSRYWLVDPLDGTKEFIKRNG
                     EFTVNIALIDGHRPVLGVVHVPVGNEVYFAALGHGSWHARAGAAPERIHVQIPCKPEV
                     RVVGSRSHGSDAMDGFINRLGDHAFVACGSSLKFCRVAEGRADVYPRLGLTSEWDTAA
                     AQCVVEEAGGHVVGLDGEALPYNTKDSLLNPYFIVYGDGSRDWLSYLPEKV"
     gene            complement(1148..1498)
                     /gene="rplS"
                     /locus_tag="PLQ18_12605"
     CDS             complement(1148..1498)
                     /gene="rplS"
                     /locus_tag="PLQ18_12605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HNE02539.1"
                     /translation="MSNIIDQLNQEQIAGKTIPDFGPGDTVVVQVKVKEGNRERLQAF
                     EGVCIARRNRGLNSAFTVRKISYGEGVERVFQTYSPSIADISVKRRGDVRRAKLYYLR
                     GLEGKAARIKEKLS"
     gene            complement(1512..>1806)
                     /locus_tag="PLQ18_12610"
     CDS             complement(1512..>1806)
                     /locus_tag="PLQ18_12610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018717804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02540.1"
                     /translation="LDCPHYTRPEEIAGLQVPAVLRSGDHAAIARWRRQQALGRTWLR
                     RRDLLAGRRLDRQDQALLDAFIRQHEERGSAAPGAVDPASQGVGAAAPDPAEP"
BASE COUNT          302 a          610 c          603 g          291 t
ORIGIN      
        1 tccagtccat cgacgggaac tccgggtgac ggcgggggcc ggcagcgtag cgcgcccggg
       61 cgttgcgtgc cgccgtcact gcgtccggcg gcccgtgcgg caggccacgt gaccgtcagg
      121 gcggtcgctc agaccttttc cggcagatac gacagccagt cgcggctgcc gtcgccgtag
      181 acgatgaagt agggattcag cagcgaatcc ttggtgttgt acgggagcgc ctcgccatcc
      241 agcccgacca catggccgcc agcttcctcg accacgcact gcgccgccgc cgtgtcccat
      301 tcggaagtca ggcccaggcg cggatagaca tcggcacggc cttcggcgac acggcagaac
      361 ttcagcgaac tgccacaggc gacgaaggca tgatccccga gacggttgat gaagccatcc
      421 atcgcatcgc tgccgtgcga gcggctgccg acgacccgca cttccggctt gcagggaatc
      481 tgcacgtgga tgcgctcggg cgcggcacca gccctggcat gccaggagcc gtgaccgagc
      541 gcggcaaagt acacctcatt gccgaccggc acatgcacga cgccgagcac cggccggtga
      601 ccgtcgatca gcgcgatgtt gaccgtgaac tcaccgttgc gcttgatgaa ctccttggtg
      661 ccatcgagcg ggtcgaccag ccagtagcgc gaccacgcgg cccgctgctc gaacggcagc
      721 gccctggatt cttcggacag caccgggatg tccggcgtca gcgccgtcag cccggcgacg
      781 atgcattcgt gcgcggcgcg atcggccgcg gtcagcgggc tgtcgtcgcc cttggtttcg
      841 acgtcgaaat tcgtatcgta gatttcgagg atgcgggcag cggcttcggc ggcgatggca
      901 cgcacgggcg tcagccactg ggcgaagtct tgggacatga agggattcct cgacagggac
      961 gaaatctggc gcgcgcacca tcgtgctgcg gaaacacggc aaagcttact gcactgcagc
     1021 atgaaaccac agccacacaa aaaccgatgc ccggcgtgaa gatgcaaacg actgacacga
     1081 agacgggcgt cgcccttgcg ggtgacgccc gtcttcgtgg atcacgcgaa tccgcggacc
     1141 gccgcgatca ggacagcttt tccttgatgc gcgccgcctt gccttcgagc ccgcgcaggt
     1201 agtagagctt ggcgcgacgg acgtcgccgc ggcgcttgac gctgatgtcg gcgatgctcg
     1261 ggctgtaggt ctggaacacg cgctcgacgc cttcgccgta cgagatcttg cgcacggtga
     1321 aggccgaatt caggccgcgg ttgcggcggg cgatgcagac gccctcgaag gcctgcagac
     1381 gctcgcggtt gccttccttg accttgacct gcacgacgac ggtgtcgccg gggccgaagt
     1441 ccgggatggt cttgccggcg atctgttcct ggttcagctg atcgatgatg ttgctcatga
     1501 aggtcactcc cctacggttc cgccgggtcc ggagccgcag cgccgacacc ctgtgatgcc
     1561 ggatccacgg cgcccggcgc cgcggatcca cgttcttcgt gctgccggat gaaggcatcc
     1621 agcagcgcct ggtcctgcct gtcgagacgc cgtccggcca gcaggtcccg ccggcgcagc
     1681 caggtacgtc cgagcgcctg ctgccggcgc cagcgcgcga tcgcggcgtg atcgcccgag
     1741 cgcagcaccg ccggcacctg cagcccggcg atctcctcgg gccgcgtgta atgcggacag
     1801 tcgagc
//
LOCUS       DAOQJH010000632         1801 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_56483_length_1801_cov_3.665521, whole genome shotgun sequence.
ACCESSION   DAOQJH010000632 DAOQJH010000000
VERSION     DAOQJH010000632.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1801)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1801)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1801
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56483_length_1801_cov_3.665521"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..823
                     /locus_tag="PLQ18_12615"
     CDS             <1..823
                     /locus_tag="PLQ18_12615"
                     /inference="COORDINATES: protein motif:HMM:NF025154.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNE02541.1"
                     /translation="STSWLRRLALLPLTPLPGGGRQPLQPLHVADLCAAVCRLLQPDA
                     PVPPGGIIEAVGPAPLTLRAYLAALRHGLGLPAAPVLPVPVGLLRLAARWLAPWRTQP
                     LCPETLTLLLRGNTGDPAALTALLDRPPRPVPAFAAGEHQSAERAESAAGLAAGLARA
                     ALALIWAAGGLASLLPGGRSAGFALLAQLGVHGPLAWLLLLGGTGLDLAFALLTLHPA
                     AGRRLWAAQAAIVALYGAIAAVGLPGLLADPLGAIVKNLALLALLGQLALHAEPD"
     gene            823..1296
                     /locus_tag="PLQ18_12620"
     CDS             823..1296
                     /locus_tag="PLQ18_12620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017977963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2269 domain-containing protein"
                     /protein_id="HNE02542.1"
                     /translation="MDYGTLKTLHVLSSTLLFGTGLGSAFYLWRAARHGDAAQFAFVA
                     RQVVLADWLFTATAVVFQPLSGWLLARTAGHPLDAGWLQASYGLYLLAGACWLPVVWL
                     QYRLRDLARDAARLQQPLPARWHHCLCLWFVLGWPAFGAVVLIFWLMVNRPGFGA"
     gene            1309..1734
                     /locus_tag="PLQ18_12625"
     CDS             1309..1734
                     /locus_tag="PLQ18_12625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020485406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF393 domain-containing protein"
                     /protein_id="HNE02543.1"
                     /translation="MHGNDPQADSPAVPSTLTLFYDGDCPICRAEVRWLKRHDRHRRL
                     RYVDVAAVDFHAPPPLPARARLLERIHLLDAGGQVHVGMAVFRLAYRAVGLGWLVAPT
                     GWPLLRPLFDRLYGGFAHHRIGLGRLFGRRCDSGSCRLR"
BASE COUNT          182 a          681 c          645 g          293 t
ORIGIN      
        1 cagcactagc tggctgcgcc ggctcgccct gctgccgctg acgccgctgc cgggcggcgg
       61 ccgccagccc ctgcagccgc tgcacgtcgc cgatctgtgc gcggcggtct gccggctgct
      121 gcagcccgat gcgcccgtcc cgcccggcgg catcatcgaa gcggtcggcc ccgcgccgct
      181 gacgctgcgc gcctacctcg ccgcactgcg ccacggcctc ggcctgccag cggcaccagt
      241 gctgccggtg ccggtcgggc tgctgcgcct cgcagcccgc tggctggcgc cctggcgcac
      301 gcagccgctc tgcccggaaa cgctgacgct gctgctgcgc ggcaacaccg gggatccggc
      361 ggcgctgacg gcgctgctcg accggccgcc gcgaccggtg ccggcgttcg cagccggcga
      421 acaccagagt gccgagcgtg cggaaagcgc cgcagggctc gcagccggcc tggcgcgggc
      481 agcgctggcg ctgatctggg ctgccggcgg actggcttcg ctgctgccgg gcggccggtc
      541 cgccggtttc gcgctgctgg ctcaactcgg tgtccacggc ccgctcgcct ggctgctgct
      601 gctcggtggc accggtctcg atctggcgtt cgcgctgctg acgctgcacc cggcggccgg
      661 tcgccggctg tgggctgcgc aggcggcgat cgtcgcgctg tacggcgcga tcgctgccgt
      721 cggcctgccc ggcctgctcg ccgatccgct cggcgccatc gtcaagaacc tcgcgctgct
      781 ggcactgctc ggccagctcg cgctgcacgc ggagccggac tgatggacta cggcacgctg
      841 aagaccctgc acgtgctgtc ctcgacgctg ctgttcggca ccggactcgg cagcgcgttt
      901 tatctgtggc gcgccgcacg tcacggcgat gccgcgcagt tcgccttcgt cgcccggcag
      961 gtcgtgctcg ccgactggct gttcacggca accgccgtcg tgttccagcc gctgagcggc
     1021 tggctgctcg cgcgcacggc cggccatccg ctggacgccg gctggctgca ggcgagctac
     1081 ggcctgtacc tgctcgccgg tgcctgctgg ctgccggtgg tctggctgca gtaccggctg
     1141 cgtgatctgg cccgtgacgc cgcgcgtctg cagcagccgc tgccggcacg ctggcatcac
     1201 tgcctgtgcc tctggttcgt cctcggctgg ccggccttcg gggcggtcgt gctgatcttc
     1261 tggctgatgg tcaaccgccc cggcttcggg gcctgaagga gcccacacat gcacggcaac
     1321 gatccgcaag ccgacagccc ggctgtcccg tccaccctga cgctgttcta cgacggcgac
     1381 tgcccgatct gccgcgccga agtgcgctgg ctcaagcgcc atgaccggca ccggcggctg
     1441 cggtatgtcg acgtggcagc cgtggatttt cacgcccctc cgccgctgcc ggcgcgcgcg
     1501 cggctgctcg aacgcatcca tctgctcgat gccggcggcc aggtgcatgt cggcatggcg
     1561 gtgttccgcc ttgcgtaccg tgcggtcggc ctcggctggc tggtggcgcc gaccggctgg
     1621 ccgctgctgc gcccgctgtt cgaccgtctg tacggcgggt tcgcgcacca ccgcatcggc
     1681 ctcggccggc tgttcggccg gcgctgcgac agcggcagct gccggctgcg ctgacagggt
     1741 tgcagacaac cggggaacgg cggggacgca gcaggcatcg aaaatcgggc tggccggctt
     1801 g
//
LOCUS       DAOQJH010000633         1798 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_56689_length_1798_cov_3.693058, whole genome shotgun sequence.
ACCESSION   DAOQJH010000633 DAOQJH010000000
VERSION     DAOQJH010000633.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1798
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56689_length_1798_cov_3.693058"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..557)
                     /locus_tag="PLQ18_12630"
     CDS             complement(<1..557)
                     /locus_tag="PLQ18_12630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008952903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HNE02544.1"
                     /translation="MTHDPFDVLRVFTTASGQHGRYCSLAALESAGLGAVSRLPVSLR
                     LVLESVLRHCDGENITEAHVRALAGWQPQAPRSEEIPFVVARVVLQDFTGVPLLCDLA
                     AMRDAAAAAGGEPGIVEPLVAVDLVVDHSVMIDHWGRPDALELNLQREYERNRERYAF
                     LKWGTQALRNFRVVPPGTGIVHQVNL"
     gene            complement(706..1410)
                     /locus_tag="PLQ18_12635"
     CDS             complement(706..1410)
                     /locus_tag="PLQ18_12635"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNE02545.1"
                     /translation="MTMNHDDPLLPAGPQPLNRDSYDAIASAWDAARRCLDEREAGWL
                     LDLVRGLRPPAPVLDLGCGSGRPLGEWLLARGLQVTGIDQSARLLALARTRLPQGRWL
                     EADIGGWIPDTDTDTDKNFAAVLIWDALFHLPRALHLPLLARIFRLLQPGGRLLLSSG
                     GSAQPAFTDTMHGARFFYDALAPDVLLAGLLDLGFGLERIEYLNPPTRGRDKGRIAVL
                     ARRPAAAPATVPHAAE"
     gene            1566..>1798
                     /locus_tag="PLQ18_12640"
     CDS             1566..>1798
                     /locus_tag="PLQ18_12640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02546.1"
                     /translation="MTTSFPPAAAAGLAPLLMPAVAELVPPAWAGYRPLIVEALDGFL
                     GRLSGARLDAIVTAQLELPEDATAAARVVARHPG"
BASE COUNT          271 a          625 c          641 g          261 t
ORIGIN      
        1 aggttgacct gatggacgat gccggtgccg ggcggcacga cgcggaagtt gcgcaaggcc
       61 tgcgtgcccc acttcaggaa ggcgtagcgc tcgcggttgc gttcgtattc gcgctgcagg
      121 ttcagttcga gcgcatcggg ccggccccag tgatcgatca tcaccgagtg atcgacgacc
      181 agatcgaccg cgaccagcgg ctcgacgatg ccgggctcgc cgccggcggc ggccgcagca
      241 tcgcgcatcg ctgccagatc gcagagcagc ggcacgccgg tgaagtcctg cagcacgacg
      301 cgcgcgacca cgaacgggat ttcctcgctg cgcggcgcct gcggctgcca gccggccagg
      361 gcgcgcacgt gcgcctcggt gatgttctcg ccatcgcagt ggcgcagcac cgattccagc
      421 accagacgca gcgacaccgg cagccgcgac acggcgccga gaccggcgct ctcgagcgcg
      481 gcgaggctgc agtaacggcc atgctggccg gaggcggtcg tgaagacgcg cagcacgtcg
      541 aacggatcgt gggtcatcgg aaggattctc cggcaacgga tcggacaggc ctgcaacctg
      601 cgactgcgca gcggtcgacg gattcccggc tgcgggctca agggcttgcg atcggtctgt
      661 cgatcagcgt aggagaggcg tccgccgcct gctccggaag cgctgctatt cggcagcgtg
      721 gggaaccgtt gccggcgctg cagcgggcct gcgcgcgagc acggcgatgc ggcccttgtc
      781 gcggccgcgg gtcggtggat tcaggtactc gatgcgttcg agtccgaagc cgagatcgag
      841 cagccctgcg agcagcacat ccggcgccag cgcgtcgtag aagaagcgcg cgccgtgcat
      901 ggtgtcggtg aaggccggct gcgcgctgcc gcccgagctg agcagcagcc ggccaccggg
      961 ctgcagaaga cggaagatgc gggccagcag cggcaggtgc agggcacgcg gcaggtgaaa
     1021 cagcgcatcc cagatgagca ccgcggcgaa attcttgtcc gtgtccgtgt ccgtgtccgg
     1081 aatccagccc ccgatgtcgg cttccagcca gcgtccctgc ggcagccggg tgcgggccag
     1141 cgccagcagg cgggcagact gatcgatgcc ggtgacctgc agcccgcgcg ccagcagcca
     1201 ttcccccagc ggccggccgc tgccgcagcc gagatcgagc accggtgccg gcggcctcag
     1261 cccgcgcacc agatcaagca gccagccggc ttcgcgctcg tccaggcaac ggcgcgcagc
     1321 atcccaggcg ctggcgatgg cgtcgtagct gtcgcggttc agtggctgcg gcccggccgg
     1381 cagcagcgga tcgtcatggt tcatcgtcat gattgcggcc tgattcctgc cttcagcatg
     1441 gccgctgccg gggccgcgat cgatggcccc ggctgcacgg gcggaaaatc cgtccgggaa
     1501 cccgcagcgc tgcgctgttt tcacttccgt atcgcatgcg atgcgccatt tcagggaatg
     1561 ccgacatgac cacctccttc ccgccggccg ctgccgccgg cctcgcgccg ctgctgatgc
     1621 ccgcggtcgc cgaactggtg ccgccggcct gggccggata ccggccgctg atcgtcgagg
     1681 cgctcgacgg cttcctcggg cggctgtcgg gcgcgcggct cgatgccatc gtcaccgcgc
     1741 agctcgaact gcccgaggat gcgacggccg ctgcgcgcgt ggtcgctcgc catcctgg
//
LOCUS       DAOQJH010000634         1798 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_56695_length_1798_cov_3.271371, whole genome shotgun sequence.
ACCESSION   DAOQJH010000634 DAOQJH010000000
VERSION     DAOQJH010000634.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1798
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56695_length_1798_cov_3.271371"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            59..406
                     /locus_tag="PLQ18_12645"
     CDS             59..406
                     /locus_tag="PLQ18_12645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015007508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HNE02547.1"
                     /translation="MASGRPYRVGLCHCLDCRKFHGALFHASAIFPEAAVTIEGEVRD
                     YEGRCFCPLCGSSVFSRSADEIELNLGSFDAPDLFVPTYESWIIRRESWLPAFPLTRR
                     YARDRDETSRFEE"
     gene            436..>1798
                     /locus_tag="PLQ18_12650"
     CDS             436..>1798
                     /locus_tag="PLQ18_12650"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNE02548.1"
                     /translation="MSADKIEQTRVIEQTRLQVEATRLREEPVEATRVLNAGNVELTL
                     LRTLNGPAGEKLTELPPLPNPRYELLGPVGQGGMASVEAARDAALRRTVAFKRLHEGV
                     QALPGLAQRFLTEAQLTAQLDHPNVVPVYALERDAHGRIAYAMKLVRGRTLAELLDEP
                     LPATRTARREARAALIEHFLKVCDALTYAHARGVIHRDLKPANIMVGAFNEVYVMDWG
                     IARIIGAPDLCAADLPVTDAGLGPDPASERTRAGDRLGTPAWMAPEQARGETVDAKSD
                     QYALGLILQQMLSRQRAIPGHDLDSVLAAAARGERRALTGEPAALVAILNRACALQPA
                     RRYADVAAFAADLRAWRADEAVSALPDTGRRRLLRWIGRHRAATAITLLASLALGAGF
                     GAWMLYRHAEALEAAGERERLVSDRLDATALQAARIESQFLQYRGLLTGIANAALLAF
                     DLPD"
BASE COUNT          252 a          656 c          645 g          245 t
ORIGIN      
        1 cgccgctgca cgttgataac cccagtcacg tgcctgcctc tgcggccggg tccggttcgt
       61 ggcgagcggg cggccgtacc gggtcggcct ctgccactgc ctcgactgcc gcaagttcca
      121 cggggcgctg ttccatgcgt ccgcgatctt cccggaggcg gcggtgacga tcgaaggcga
      181 ggtccgcgac tacgaaggcc gctgcttctg tccgctttgc ggatcgtcgg tgttctcgcg
      241 cagcgccgat gagatcgagc tgaacctcgg ttcgttcgat gcccccgacc tgttcgtgcc
      301 gacctacgaa agctggatca tccgccgcga atcctggctg ccggcatttc cgctcacgcg
      361 ccgctacgcg cgggatcggg atgagacgag ccggttcgag gaatagatgc ggacaaagcc
      421 ccgggtgccg ccggcatgag cgccgacaaa atcgagcaga cccgcgtgat cgaacagacc
      481 cgcctgcagg tcgaggccac ccggctgcgg gaagagcccg tcgaggcgac ccgcgtgctc
      541 aatgccggca acgtcgaact gacgctgctg cgcaccctga acggcccggc cggcgaaaag
      601 ctcaccgagc tgccgccgct gccgaacccg cgctacgaac tgctcggccc ggtcgggcag
      661 gggggaatgg cgagcgtcga ggcggcgcgc gatgcggcgc tgcggcgcac ggtcgccttc
      721 aagcgcctgc acgaaggcgt gcaggcgctg ccggggctcg cgcagcgctt cctgaccgag
      781 gcgcagctga ccgcgcagct cgatcacccg aacgtggtgc cggtctatgc gctggagcgc
      841 gacgcgcacg ggcgcatcgc ctacgcgatg aagctcgtgc gcgggcgcac gctggccgaa
      901 ctgctggacg aaccgctgcc ggcgacgcgc acggcccgcc gcgaggcgcg cgcggcgctg
      961 atcgagcact tcctgaaggt ctgcgatgcg ctgacctacg cgcatgcgcg cggcgtcatc
     1021 caccgcgatc tgaagcccgc caacatcatg gtcggcgcct tcaacgaggt gtacgtgatg
     1081 gactggggca tcgcccgcat catcggtgcc ccggacctct gcgctgccga cctgccggtc
     1141 accgacgccg ggctcggtcc tgatccggcc agcgagcgca cccgcgccgg cgaccggctc
     1201 ggcacgccgg cctggatggc gcccgagcag gcgcgcggcg agacggtcga tgcgaagagc
     1261 gaccagtacg cgctcgggct gatcctgcag cagatgctga gccgccagcg cgcgatcccc
     1321 ggccatgacc tcgacagcgt gctcgccgcc gccgcccggg gtgagcgccg ggcgctgacc
     1381 ggcgagccgg cggcgctcgt ggcgatcctg aaccgcgcct gcgcgctgca gccggcgcgc
     1441 cgctacgccg atgtcgcggc gtttgcggcc gacctgcgcg cctggcgggc cgacgaggcc
     1501 gtcagcgcgc tgcccgacac cggccgccgg cgcctgctgc gctggatcgg ccgccaccgc
     1561 gccgcgaccg cgatcacgct gctggcgagc ctcgcgctcg gggccggctt cggggcctgg
     1621 atgctgtacc ggcacgccga ggcactcgaa gccgccggcg agcgggagcg gctggtcagc
     1681 gaccggctgg atgcgaccgc gctgcaggcg gcgcgcatcg agtcgcagtt cctgcagtat
     1741 cgcggcctgc tgaccggcat cgccaacgcg gcgctgctgg ccttcgacct gccggatg
//
LOCUS       DAOQJH010000635         1795 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_56870_length_1795_cov_4.079310, whole genome shotgun sequence.
ACCESSION   DAOQJH010000635 DAOQJH010000000
VERSION     DAOQJH010000635.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1795)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1795)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1795
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56870_length_1795_cov_4.079310"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..909
                     /locus_tag="PLQ18_12655"
     CDS             <1..909
                     /locus_tag="PLQ18_12655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015451767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNE02549.1"
                     /translation="AWRQIRTVLGMTAGILLLNLFVYLPVRRALRPSEQILAVMEKMR
                     AGDLSVRMPRPALHELQRIASGFDELADRLQQTLDEQRRLAQRLIAVREEERRHLGRE
                     LHDEFGQCLTSIGAEAVFIGGQVRERLPELLPAVGSISEVTAKMLEALQGILSQLRPV
                     GLESFGLQAILEQFLAGWQRRQPDCRFTLDLDGPLDDLPDELTVSLYRIVQESLTNAL
                     RHGSPTQVTVQLQRHDRHCRLCIEDDGEAGENPTAGSGLGVLGMRERVLALGGRFSMT
                     PRSPRGMRVLAEFPAESLSAERSFHA"
     gene            902..1555
                     /locus_tag="PLQ18_12660"
     CDS             902..1555
                     /locus_tag="PLQ18_12660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015451766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNE02550.1"
                     /translation="MPDPIRVLLIDDHVVVRVGYRRLLEAQPDIRVAGEAATAREALD
                     WLRQQEAAATEPELVILDLGLPDLGGVELIRRLLQRQPRLRILVFTMHREPIFATQAL
                     RAGALGYVTKSSPPEVLIDAVYRVAARRQTISPDIAPDLALALIAPPEKPLAESLSPR
                     EFEILRLLLEGSSPEEIGARLSISAKTVQNCHYQIKTKLGARSDIELTRIALRAGLI"
BASE COUNT          291 a          637 c          602 g          265 t
ORIGIN      
        1 gcctggcggc agatccgcac cgtgctcggc atgaccgccg gcatcctgct gctgaacctg
       61 ttcgtgtatc tgccggtgcg ccgggcgctg cgcccgagcg agcagattct cgcggtcatg
      121 gagaagatgc gcgccggcga cctgtcggtg cgcatgccac gcccggccct gcacgagctg
      181 cagcgcatcg ccagcggctt cgacgagctt gccgaccggc tgcagcagac gctcgacgaa
      241 cagcgccggc tggcgcagcg gctgatcgcc gtgcgcgagg aggaacgccg ccacctcggc
      301 cgcgagctgc acgacgaatt cggccagtgc ctgacctcga tcggcgccga ggcggtgttc
      361 atcggcggtc aggtgcgcga gcgcctgccc gagctgctgc cggcggtcgg ctcgatcagc
      421 gaggtgacgg cgaagatgct ggaggcgctg cagggcatcc tgagccagtt gcgcccggtc
      481 gggctggaat ccttcggcct gcaggcgatc ctcgaacagt tcctggccgg ctggcagcgc
      541 cgccagccgg actgccgctt cacgctcgat ctcgacggcc cgctcgacga cctgcccgat
      601 gaactcaccg tcagcctcta ccgcatcgtg caggagagcc tgaccaatgc cctgcgccac
      661 ggctcgccca cgcaggtgac ggtgcagctg cagcgccatg accggcactg ccggctctgc
      721 atcgaggacg acggcgaggc cggtgaaaat ccgacggccg gcagcggact cggcgtgctc
      781 ggcatgcgcg agcgcgtcct cgcgctgggc gggcggttct cgatgacgcc gcgcagcccg
      841 cgcggcatgc gcgtgctggc cgaattcccg gccgaatccc tcagtgccga gcggagcttc
      901 catgcctgat cccatccggg tgttgctgat cgacgatcac gtcgtggtcc gcgtcggcta
      961 ccggcgcctg ctggaggcgc agcccgacat ccgcgtcgcc ggcgaagccg cgaccgcgcg
     1021 cgaggcgctg gactggctgc ggcagcagga ggccgcggcc acggaacccg aactggtgat
     1081 cctcgacctc ggcctgcccg atctcggcgg cgtcgaactg atccgccggc tgctgcaacg
     1141 ccagccgcgc ctgcgcatcc tggtgttcac gatgcaccgc gagccgatct tcgcgacgca
     1201 ggcgctgcgc gccggcgccc tcggctacgt gaccaagagc agcccgccgg aagtgctgat
     1261 cgacgccgtg taccgggtcg ccgcgcgccg ccagacgatc agcccggaca tcgctccgga
     1321 tctggctctg gcgctgatcg cgccgccgga aaaaccgctc gcggaatcac tgtcgccgcg
     1381 cgaattcgaa atcctgcgcc tgctgctgga aggcagcagc cccgaggaaa tcggcgcgcg
     1441 actgtcgatc agcgccaaga ccgtgcagaa ctgccattac cagatcaaga ccaaactcgg
     1501 cgcccgcagc gacatcgaac tgacgcgcat cgccctgcgc gccgggctga tctgatgccg
     1561 gctcatgccg atcacaaatg aacacgccgt cttttcagcc cctcgcccct cgcgggagag
     1621 ggcttgggga gagggaaaaa ccacgcattt acgtgtgatc ggtattacgc cgcctgcgcc
     1681 cggtacaact cgaccagcat cggccaggta acccgcgcga gcggattgcc ggcatcgtcg
     1741 agcagttcat acaggtcatc ggcaccatcg gggcggatgc tgaaatgcac gatgg
//
LOCUS       DAOQJH010000636         1793 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_56993_length_1793_cov_2.807250, whole genome shotgun sequence.
ACCESSION   DAOQJH010000636 DAOQJH010000000
VERSION     DAOQJH010000636.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1793
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56993_length_1793_cov_2.807250"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..741
                     /locus_tag="PLQ18_12665"
     CDS             <1..741
                     /locus_tag="PLQ18_12665"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010627131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HNE02551.1"
                     /translation="LLGAGLLHGDVLTVAGPGLARYAEEPWLDGDRLAWRDAPAESGD
                     DSVLRPAAAPFAADGGLKLLHGNLGRAVIKTSAVAGKHRVIEAPARVFNGQEAVIAAF
                     EAGELEHDVVVVVVGQGPRANGMPELHHLTPALSVLLDRGYRVALLTDGRMSGASGKV
                     PAAIHVTPEVLSGGPLGKVRDGDLIRLDSTSGELVALVDPETWAGRELRLPDVSAHQH
                     GLGRELFAGFRRLAHGAEQGGGIFFADV"
     gene            868..1482
                     /gene="eda"
                     /locus_tag="PLQ18_12670"
     CDS             868..1482
                     /gene="eda"
                     /locus_tag="PLQ18_12670"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008675 -
                     2-dehydro-3-deoxy-phosphogluconate aldolase activity
                     [Evidence IEA];
                     GO_function: GO:0008700 - 4-hydroxy-2-oxoglutarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0009084 - glutamine family amino acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HNE02552.1"
                     /translation="MDIRTVMRLAPVIPVIVVDSPEQAVPLARALVAGGLPVLEVTLR
                     TAAALPAIEAIAAEVEGAVLGVGTVVRPAQFEAARRAGATFAVTPGLTPTLAAAAKDC
                     GLPLLPGVMTPGEVIAAGEAGFDALKLFPAAQAGGIGMLQALAGPFPDVVFCPTGGFT
                     ADNFRAALALPNVLCVGGSWVVPKAAIAAGDWATITALARATRD"
     gene            1558..>1793
                     /locus_tag="PLQ18_12675"
     CDS             1558..>1793
                     /locus_tag="PLQ18_12675"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HNE02553.1"
                     /translation="MPGKVFVSYSKQDKKLKDRVVQQLKVLAGEGLEVWTDADIPAGA
                     DWLPAIEKAMAECQVALLLISSGFLTTGFVTEKEV"
BASE COUNT          213 a          657 c          666 g          257 t
ORIGIN      
        1 ctgctcgggg ccggcctgct gcacggcgac gtgctgacgg tcgccgggcc ggggcttgcg
       61 cgctacgccg aggaaccctg gctcgatggc gaccggctcg cgtggcgcga cgccccggcc
      121 gaaagcggcg atgactcggt gctgcgcccg gccgccgcgc cgtttgcggc cgatggcggc
      181 ctcaagctcc tgcacggcaa cctcggccgc gccgtcatca agacctcggc ggtcgccggg
      241 aagcaccgcg tcatcgaggc gccggcgcgg gttttcaacg gtcaggaggc ggtgatcgct
      301 gccttcgagg ccggcgagct cgagcacgat gtcgtcgtcg tcgtcgtcgg tcaggggccg
      361 cgcgccaacg gcatgccgga gcttcatcac ctgacgccgg cgctgagcgt gctgctcgat
      421 cgcggctacc gcgtcgcgct gctgaccgac gggcgcatgt ccggcgcctc cggcaaggtg
      481 ccggcggcga tccacgtcac gcccgaggtg ctctcgggcg ggccgctcgg caaggtccgc
      541 gacggcgacc tgatccgcct cgacagcacg agcggcgaac tcgtcgccct cgtcgatccg
      601 gagacctggg ccggccgcga gctgcgcctg ccggacgtga gtgcccacca gcacggcctc
      661 ggccgcgagc tgttcgcggg cttccggcgg ctcgcgcacg gtgcggagca gggcggcggc
      721 atcttcttcg ccgacgtctg agcgccggat cttcctgccg tccccggccc tgcattcctt
      781 gcggatccag ggccggctgc gggccgcccg ccgcgcccgc gccgctcctt cgaccgccac
      841 cctccgccga cccgaggaac cgcctccgtg gacatccgca ccgtcatgcg cctcgcgccg
      901 gtcattccgg tcatcgtcgt cgattcgccg gagcaggccg tgccgctggc gcgggcgctg
      961 gtggccggcg ggctgccggt gctcgaagtc acgctgcgca cggccgcggc gctgccggcg
     1021 atcgaggcga tcgccgccga ggtcgagggc gcggtgctcg gtgtcggcac ggtcgtgcgc
     1081 ccggcgcagt tcgaagccgc gcgccgggcc ggcgccacct tcgcggtcac gcccggcctg
     1141 acgccgacgc tcgccgccgc ggcgaaggac tgcggcctgc cgctgctgcc cggcgtcatg
     1201 acgccgggcg aggtcatcgc cgccggcgag gccggtttcg atgcgctgaa gctgttcccg
     1261 gccgcgcagg ccggcggcat cggcatgctg caggcgctcg ccggtccgtt cccggacgtg
     1321 gtcttctgcc cgaccggcgg cttcacggcc gacaacttcc gcgccgcgct ggcgctgccg
     1381 aacgtgctgt gcgtcggcgg ctcctgggtg gtgccgaagg ccgcaatcgc ggccggcgac
     1441 tgggcgacga tcacggcgct ggcgcgggcg acgcgcgact gaacccccgc cgcccgcatt
     1501 gccccgcccc ggcgccggtc gttacgctcg accggcgatg gaatgggagg gtgcgcgatg
     1561 ccgggcaagg tgttcgtcag ttacagcaag caggacaaga agctgaagga tcgcgtcgtg
     1621 cagcaactga aggtgctggc cggcgagggg ctggaggtct ggaccgatgc cgacatcccg
     1681 gccggtgccg actggctgcc ggcgatcgag aaggcgatgg ccgaatgcca ggtggcgctg
     1741 ctgctgatct ccagcggctt tctgaccacc ggcttcgtca ccgaaaagga agt
//
LOCUS       DAOQJH010000637         1787 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_57370_length_1787_cov_2.928984, whole genome shotgun sequence.
ACCESSION   DAOQJH010000637 DAOQJH010000000
VERSION     DAOQJH010000637.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1787)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1787)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1787
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57370_length_1787_cov_2.928984"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..921)
                     /locus_tag="PLQ18_12680"
     CDS             complement(<1..921)
                     /locus_tag="PLQ18_12680"
                     /inference="COORDINATES: protein motif:HMM:NF024589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HNE02554.1"
                     /translation="MPGKVKVFISYSHQDERYKDALEKHLGVAADRLEVWSDRRIEAG
                     ADWLAEIKQALAGCRVALLLISADFLTSPFILGTEVPALLRRREKQGLRVIPVIVQHC
                     IWDEVGWLKPIQARPLDGVALADLSKARCNKVLADLAREVLRLSAGTQQRPARRAPGL
                     SEPEQPPVIDIDRLPAGTDGFFGRVHELSQLDRLWQRDRNAALCVLVAPGGTGKTALV
                     QRWLARLRARHWCGAQRVFGWSFYSQGQADDRQVSEEPFLAAALAFFGVRVGAALPAH
                     AKGERLADAIAGTRTLLILDGLEPLQYPPGP"
     gene            complement(1010..1534)
                     /locus_tag="PLQ18_12685"
     CDS             complement(1010..1534)
                     /locus_tag="PLQ18_12685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014088402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HNE02555.1"
                     /translation="MTRIRAVLFDWGNTLMRDLPGQHGPMRDWPHVEALPGALEALEA
                     LRAQGLRLALATNAADSGPADIRAALARCGLSAALEQIFCARELGLAKPARAFYGACL
                     AGLDLPAEAVAMVGDDWHGDVQGALHAGLAAVWLQARRSLLPPVPDGACVTTIAALGE
                     LPELFTHWPPVQDD"
BASE COUNT          240 a          679 c          631 g          237 t
ORIGIN      
        1 cgggccgggc gggtattgca gcggctccag cccgtcgagg atcagcagcg tgcgcgtgcc
       61 ggcgatcgca tcggccagcc gctcgccctt cgcatgcgcc ggcagcgccg caccgaccct
      121 gaccccgaag aaggccagcg ccgcggccag aaacggctct tccgacacct gccggtcatc
      181 ggcctgcccc tggctgtaga agctccagcc gaacacgcgc tgcgcgccac accagtgccg
      241 cgcgcgcagg cgcgccagcc agcgctgcac cagcgccgtc ttgccggtgc cgccgggcgc
      301 gaccagcacg cacagcgccg cattacggtc gcgctgccag agccggtcga gctgcgagag
      361 ttcgtgcacg cggccaaaga agccgtcggt gccggccggg aggcggtcga tgtcgatgac
      421 gggcggctgt tccggctcgg acaggcccgg cgcgcgccgc gccgggcgct gctgcgtgcc
      481 ggcgctcagg cgcaggacct cgcgcgccag atcggccagc accttgttgc atctggcctt
      541 gctcaggtca gcgagcgcca cgccgtcgag cggtcgggcc tggatcggct tcagccagcc
      601 gacttcatcc cagatgcagt gctggacgat gaccggaatc acccgcagcc cctgcttctc
      661 gcgccgcctg agcagcgccg gcacttcggt gccgaggatg aacggcgagg tcaggaagtc
      721 tgccgagatc agcagcagcg caacgcgaca gccggcgagg gcctgtttga tctccgccag
      781 ccagtccgca ccggcctcga tccggcggtc gctccagact tcgagccggt cagcggcgac
      841 gcccaggtgt ttctccagcg catccttgta gcgctcgtcc tgatggctgt agctgatgaa
      901 taccttgacc ttgcccggca tcgcgtgccc ccttccttct gccggccgag cgtaacggcc
      961 ggcgcagggg cgggacaatg cgggcgggcg cggaccggtc ggcctcgcct cagtcgtcct
     1021 gcaccggcgg ccagtgcgtg aacagctccg gcagttcgcc gagcgcggcg atcgtggtca
     1081 cgcacgcgcc gtccggcacc ggcggcagca ggctgcgccg ggcctgcagc cagacggcgg
     1141 cgagccccgc gtgcagcgcg ccctgcacgt cgccgtgcca gtcgtcaccg accatcgcca
     1201 ccgcctcggc cggcaggtcg agcccggcga ggcaggcgcc gtagaaggcg cgggccggtt
     1261 tggcgaggcc gagttcgcgg gcgcagaaga tctgttcgag cgccgccgac agcccgcagc
     1321 gcgccagcgc ggcgcggatg tcggcggggc cggagtcggc ggcgttggtc gcgagcgcca
     1381 gccgcaggcc ctgcgcgcgc agcgcttcga gcgcttcgag cgcgccgggc agcgcctcga
     1441 cgtgcggcca gtcgcgcatc ggcccgtgct ggccgggcag gtcgcgcatc agcgtgttgc
     1501 cccagtcgaa cagcaccgcc cggattctcg tcatcgcccg tcgccccgcc ctgccgatcc
     1561 cgatcccgat ccggcgggga aatttccccg cctttacccg cgtacccgcg gggacgggcg
     1621 caagttcagc ccggccccgt cacgccccgg ctcgccgtgc cgatccgctg acccgcgccg
     1681 tgatgccggg gcttctgcat cggcatcagc gcggtccgcg gttggcgagc cacacggcgc
     1741 cggccaccag cgccatgccg aggatagcca cggccccgag gcgttcg
//
LOCUS       DAOQJH010000638         1785 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_57495_length_1785_cov_3.798266, whole genome shotgun sequence.
ACCESSION   DAOQJH010000638 DAOQJH010000000
VERSION     DAOQJH010000638.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1785)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1785)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1785
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57495_length_1785_cov_3.798266"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..637
                     /locus_tag="PLQ18_12690"
     CDS             <1..637
                     /locus_tag="PLQ18_12690"
                     /inference="COORDINATES: protein motif:HMM:NF017319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNE02556.1"
                     /translation="MHSILHRLLKPLCALLLGIGLFAAAGPGTAADTPAAQAVEDTAS
                     RMLVALKQQREALRADPSKIYGLVERIAVPRFDFEAISQWVLGRNWRGASPEQRQRFT
                     AEFQNLLVRIYSNALLEYSDETVRFLPGAAPNADGDVLVRSEFVRRAGPAVPINYSVH
                     QKNGQWLVYDVTVDGVSLVANYRTTFANQVRDQGMDGLIASLAKLNASGGR"
     gene            639..959
                     /locus_tag="PLQ18_12695"
     CDS             639..959
                     /locus_tag="PLQ18_12695"
                     /inference="COORDINATES: protein motif:HMM:NF024857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNE02557.1"
                     /translation="MSTGAAAVQPATLTRLGPGRLALGGDLVIDVVAALWDAAAGELA
                     GGETVTLSLDGVRRADSAGVALLVEWLRAARAGGGELRLSGLPAQMRAILAVSDLDTL
                     FPDA"
     gene            1053..1304
                     /locus_tag="PLQ18_12700"
     CDS             1053..1304
                     /locus_tag="PLQ18_12700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008208007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA/IbaG family iron-sulfur metabolism protein"
                     /protein_id="HNE02558.1"
                     /translation="MQAADIQRLIQEALPSAEVLVRGDDGVHFEAVVISAEFAGKSLI
                     AQHRLVNAALGDRLQREEIHALGLRTFTPEAWAAQQAQA"
     gene            1318..>1785
                     /locus_tag="PLQ18_12705"
     CDS             1318..>1785
                     /locus_tag="PLQ18_12705"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010961211.1"
                     /note="adds enolpyruvyl to UDP-N-acetylglucosamine as a
                     component of cell wall formation; gram-positive bacteria
                     have 2 copies of MurA which are active; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HNE02559.1"
                     /translation="MSKLIITGGETLRGEVRACGAKNAVLPILAATLLADGPVTIGNV
                     PHLQDVTTTMELLGRLGAQLVVDEKLNIEVDPRCITSHTAPYELVRQMRASILVLGPL
                     LARFGEAEVSLPGGCAIGSRPVNLHIKGLAAMGADIRVEEGYIRARCPRLKGAR"
BASE COUNT          252 a          648 c          616 g          269 t
ORIGIN      
        1 catgcattcc atcctgcacc gactcctgaa accgctgtgc gcgctgctgc tcggcatcgg
       61 cctcttcgcc gccgccgggc cgggcacggc ggccgacacc ccggcggcac aggcggtcga
      121 ggacaccgcc agccgcatgc tggtggcact gaagcagcag cgcgaggcgc tgcgcgccga
      181 tccgtcgaag atctacggcc tggtcgagcg catcgccgtg ccgcgcttcg acttcgaggc
      241 gatctcgcag tgggtgctcg gccgcaactg gcgtggcgcg agtcccgagc agcgccagcg
      301 tttcacggcc gagttccaga acctgctggt gcgcatctat tcgaatgcgc tgctcgaata
      361 ctcggacgag accgtgcgct tcctgcccgg cgccgcgccg aacgctgacg gcgacgtgct
      421 ggtgcgctcg gagttcgtgc gccgcgccgg cccggccgtg ccgatcaact acagcgtcca
      481 tcagaagaac ggccagtggc tggtctacga cgtgaccgtc gacggggtca gtctggtcgc
      541 caactaccgc acgaccttcg ccaatcaggt ccgcgaccag ggcatggacg ggctgatcgc
      601 ctcgcttgcg aagctcaacg ccagcggcgg caggtgacgt gagcactggc gcagcggccg
      661 tgcagccggc gacgctgacc cgcctcgggc ccggccggct ggcgctcggc ggcgatctgg
      721 tcatcgacgt ggtcgctgcg ctgtgggacg ccgcggccgg ggaactcgcc ggcggcgaga
      781 cggtgacgct gagtctcgac ggcgtgcgcc gtgccgacag tgccggcgtc gcgctgctgg
      841 tcgagtggct gcgtgccgcg cgtgccggcg gcggcgaact gcgcctgagc ggactgccgg
      901 cccagatgcg cgcgatcctc gccgtctcag acctcgacac actgtttccg gacgcctgag
      961 tccggcggtt ttccgatatc atcccgcctc ttgcggtacg ccctccgggg cgccggccgc
     1021 ctttcccctt tcgattccac aggtatccgc ccatgcaggc cgccgacatc cagcgtctga
     1081 tccaggaagc cctgccgtct gccgaggtgc tcgtgcgcgg cgatgacggc gtgcacttcg
     1141 aggccgtcgt catcagcgcc gagttcgccg gcaagtcgct gatcgcccag caccggctgg
     1201 tcaacgccgc gctcggcgat cgcctgcagc gcgaggagat ccacgcgctc ggcctgcgca
     1261 ccttcacgcc cgaggcctgg gcggcacagc aggcgcaggc ctgaccaggg atcgcgcatg
     1321 agcaagctga tcatcaccgg cggcgagacg ctgcgcggtg aagtgcgcgc ctgtggcgcc
     1381 aagaacgccg tgctgccgat cctggccgcg acgctgctcg ccgacggtcc ggtcacgatc
     1441 ggcaacgtcc cgcacctgca ggacgtgacc acgaccatgg aactgctcgg ccggctcggc
     1501 gcgcagctgg tcgtcgacga gaagctcaac atcgaggtcg atccgcgctg catcaccagc
     1561 cacaccgcgc cctacgagct ggtgcgccag atgcgcgcgt cgatcctcgt gctcggaccg
     1621 ctgctcgcgc gcttcggcga ggccgaggtg tcgctgcccg gcggctgcgc gatcggctcg
     1681 cggccggtga acctgcacat caagggtctc gcggcgatgg gcgccgacat ccgcgtcgag
     1741 gaaggctaca tccgcgcccg ctgcccgcgc ctgaagggcg cgcgc
//
LOCUS       DAOQJH010000639         1783 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_57655_length_1783_cov_3.009259, whole genome shotgun sequence.
ACCESSION   DAOQJH010000639 DAOQJH010000000
VERSION     DAOQJH010000639.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1783)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1783)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1783
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57655_length_1783_cov_3.009259"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..175
                     /locus_tag="PLQ18_12710"
     CDS             <1..175
                     /locus_tag="PLQ18_12710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HNE02560.1"
                     /translation="KNSSLAVAIGYPDLVSVTNTTINQTGQAIEGIAIVMAAYLSVNL
                     AVAGLIALWGRGR"
     gene            620..1729
                     /locus_tag="PLQ18_12715"
     CDS             620..1729
                     /locus_tag="PLQ18_12715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M4 family metallopeptidase"
                     /protein_id="HNE02561.1"
                     /translation="MSRPSRPETAPCAARLTPHHRHAAGCGCFIIPTRVLDRFAQDEQ
                     LPEAQRKSFADAASIETEWRKVRSASQQLMRMAHSVLPTGMTALAAAAPPVVSVFDCR
                     HGMALPGTPFCDPAHATDETARRTFAETTAMAEFLRLAFNRNSLDDAGMGLLSSIHFS
                     VGYNNAFWNGLQMTYGDGDGNIFIDFTRSNDVIAHELTHGLTEYTAGFAYRNQAGGLN
                     ESVSDVFGSMFRQWRAGQSVQQADWLIGRDIMGPGALARGYTCLRDLANPGAEHCLSP
                     QPAHFDQYRNGMDPHDSSGIPNQAFYRAAMAIGGHSWEKTGRIWYHALTGYRPSPNLS
                     MRSFANRTRSLARQIFGSEPSIRRAVDQAWLAVGL"
BASE COUNT          317 a          633 c          558 g          275 t
ORIGIN      
        1 caagaacagc tcgctcgcgg tcgcgatcgg ctatccggat ctggtttcgg tcaccaacac
       61 caccatcaac cagaccggac aggcgatcga aggcatcgcg atcgtcatgg cggcctacct
      121 gagcgtgaac ctcgcggtcg cggggctgat cgcgctgtgg ggcaggggac gctgatgcgc
      181 cgcgccgcgg gcttgtccgg acgggtccgg gtctgcaggg aaaagtccgg attcgtgatc
      241 gtcattcctg cggcccggca ttagccgccg cccagggtcc gggtgctccg gacggccttg
      301 cgcatgaagt ttccttcatg tccggagaaa gccgcgccgg atactgcgcc tgcagtatgg
      361 gattgtcgga atcgagcact aggctcatcg ctgccggctg cggcatccgc aaatgtccga
      421 cagccggaaa agccggtcac gcgcccgaat ggcatttcga ttcattcccc cctgcctgtc
      481 tgcaggggag gaatccgcaa gccggacgtg aacggcacat cgacgattcc ggcctgaacg
      541 ccgcggccgt gggttgcaac acggcggcca cgttccagaa gccggacgca tgacggccac
      601 cgccatcagg agtccgaaca tgtcccgtcc gtcccgtccc gaaaccgcac cgtgcgccgc
      661 gagactcacg ccccatcacc gccacgcggc cggctgcggc tgcttcatca tcccgacccg
      721 ggtgctcgac cgcttcgcgc aggacgagca actgcccgag gcacaacgca aatccttcgc
      781 cgatgccgcg agcatcgaga ccgaatggcg caaggtgcgc agcgccagcc agcagctgat
      841 gcgcatggcg cacagcgtgc tgccgaccgg catgaccgcg ctcgccgccg ccgcgccgcc
      901 ggtggtgagc gtcttcgact gccgccacgg catggcgctg cccggcacgc ccttctgcga
      961 tcccgcccat gccaccgacg agaccgcccg ccgcaccttc gctgaaacca cggccatggc
     1021 cgaattcctg cgcctggcct tcaaccgcaa ttcgctcgac gatgccggca tgggcctgct
     1081 gtcgtcgatc catttcagtg tcggctacaa caacgccttc tggaatggcc tgcagatgac
     1141 ctacggtgat ggcgacggca acatcttcat cgatttcacg cgctccaatg acgtgatcgc
     1201 gcacgaactg acgcacggcc tgaccgaata cacggccggc ttcgcctatc gcaatcaggc
     1261 cggcggactc aatgaaagcg tgtcggacgt attcggttcg atgttccggc aatggcgcgc
     1321 cggccagagc gtgcagcagg ccgactggct gatcggacgc gacatcatgg gaccgggcgc
     1381 gctggcgcgc ggctacacct gcctgcgcga cctggccaat ccgggtgccg agcactgcct
     1441 gtcgccgcag ccggcgcatt tcgaccagta ccgcaacggc atggacccgc acgacagcag
     1501 cggcatcccg aaccaggcct tttatcgggc ggcgatggcg atcggcgggc acagctggga
     1561 gaagaccggc cgcatctggt accacgcgct gaccggctac cgcccgagcc cgaacctgtc
     1621 gatgcgcagc ttcgcaaacc gcacgcgcag cctcgcacgc cagatcttcg gcagcgaacc
     1681 gtcgatccgc cgcgccgtcg atcaggcctg gctggcggtc ggcctataat cggcgcccgc
     1741 acgcgcaccg ggagatccgc gatgacgatg ctgcacgtcg agc
//
LOCUS       DAOQJH010000640         1782 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_57703_length_1782_cov_4.771859, whole genome shotgun sequence.
ACCESSION   DAOQJH010000640 DAOQJH010000000
VERSION     DAOQJH010000640.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1782)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1782)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1782
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57703_length_1782_cov_4.771859"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            95..1603
                     /locus_tag="PLQ18_12720"
     CDS             95..1603
                     /locus_tag="PLQ18_12720"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNE02562.1"
                     /translation="MDETLLARLGACTHLPTLPAVAMRIIELGDDPDAGIGEIADVVC
                     RDPALTAKLLKIANSPLYAQRRKVENLRQAMMLLGLNAAMTLALSFSLVGGFGASAGG
                     LDHLRYWRRSLLTALASRTIGLQAGLGQHREELFLAGLLQDIGILVLDAAAPQDYARG
                     LDACPGHLALAQAEQAVFGADHAEVGSWMLDRWGMPEYLVLATRFSHVLTAGALADSG
                     ENARFFACTTLAGWLAEVWLNPPDEAGTARIGMLAQDLLGLDAAAVTALVDEIGAQIP
                     ETSALFEIELPDAAQVTAVLDHAREVLMVRNLQIVQEAAQARRQAEHMQERARLLEER
                     SRRDPLTGAYNRSSLQETLASEFRLATEGNWPLTLAFIDLDHFKRVNDTFGHQAGDEV
                     LCEIVRRLNAHIRCTDIVARFGGEEFVLVLPGLRRNAALTMLERVRAAIAAEPCRLAE
                     DQQVTVTLSIGIATHGEDKPYESAESLLRNADRAVYDAKRNGRNRVECYRDD"
BASE COUNT          272 a          638 c          607 g          265 t
ORIGIN      
        1 cgagggtcac gcggcggtta tgataggctc ggtccggaca cgccgcctgc cgctcgtgtc
       61 gcatccttat gaaagtcgag gacgccgtac gccgatggat gaaaccctgc tagcccgact
      121 cggtgcctgc acgcacctgc cgacgctgcc cgccgtcgcg atgcgcatca tcgaactcgg
      181 cgacgacccg gatgccggca tcggcgagat cgccgacgtg gtctgccgtg acccggccct
      241 gaccgcgaag ctgctgaaga tcgccaattc gccgctgtac gcgcagcggc gcaaggtcga
      301 gaacctgcgc caggcgatga tgctgctcgg cctcaacgcc gcgatgacgc tggcgctgag
      361 cttctcgctg gtcggcggct tcggggcctc ggccggtggg ctcgaccacc tgcgctactg
      421 gcggcgctcc ctgctgacgg cgctggccag ccgcacgatc ggcctgcagg ccggtctggg
      481 gcagcaccgc gaggaattgt tcctggccgg cctgctgcag gacatcggca tcctcgtgct
      541 cgatgccgcg gcaccgcagg actacgccag agggctggac gcctgcccgg ggcatctggc
      601 gctggcgcag gccgagcagg cggtgttcgg tgccgatcac gccgaggtcg ggtcctggat
      661 gctggatcgc tggggcatgc ccgaatatct ggtgctcgcc acgcgcttca gtcatgtcct
      721 caccgccggg gcgctggccg acagcggcga gaatgcccgc ttcttcgcct gcacgacgct
      781 ggccggctgg ctcgccgagg tctggctgaa tccgcccgac gaggccggca ccgcgcgcat
      841 cggcatgctg gcgcaggacc tgctcggact cgatgcggcg gccgtcaccg cgctggtcga
      901 tgagatcggt gcgcagattc cggaaacctc ggcgctgttc gagatcgagc tgcccgatgc
      961 cgcgcaggtg acggccgtgc tcgatcacgc ccgcgaggtg ctgatggtgc gcaacctgca
     1021 gatcgtccag gaggccgccc aggcgcgccg ccaggccgag cacatgcagg aacgcgcccg
     1081 cctgctggag gaacgctcgc gccgcgatcc gctgaccggg gcctataacc gcagctcgct
     1141 gcaggagacg ctggcgagcg aattccggct cgcgaccgaa ggcaactggc cgctgacgct
     1201 ggccttcatc gatctcgatc acttcaagcg cgtcaacgac accttcggcc atcaggccgg
     1261 cgacgaggtg ctctgcgaga tcgtccggcg cctgaacgcg cacatccgct gcaccgacat
     1321 cgtcgcgcgc ttcggcggcg aggaattcgt gctggtgctg ccgggcctgc gccgcaacgc
     1381 cgcgctgacg atgctggagc gcgtgcgcgc cgccattgcc gcagaaccct gccggctggc
     1441 cgaggaccag caggtgacgg tgacgctgtc gatcggcatc gccacccacg gcgaggacaa
     1501 gccgtacgaa tcggccgaga gcctgctgcg caacgccgat cgcgccgtct acgacgccaa
     1561 gcgcaacggc cgcaaccgcg tcgagtgcta ccgcgacgac tgaagcgccc cggcatggtt
     1621 ggccggaagg cgggtcgata gactaacggg cctctccgtc cggatgcccg gtcccgtacc
     1681 gctcctccat cagccaggtc gcaagccatg gacacgatgc gtatcgcccc gctgcatgcc
     1741 gcacccccgg cccggaaatc cgccggcccc ggcgccgtcc tg
//
LOCUS       DAOQJH010000641         1774 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_58255_length_1774_cov_3.381617, whole genome shotgun sequence.
ACCESSION   DAOQJH010000641 DAOQJH010000000
VERSION     DAOQJH010000641.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1774)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1774)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1774
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58255_length_1774_cov_3.381617"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1101)
                     /locus_tag="PLQ18_12725"
     CDS             complement(<1..1101)
                     /locus_tag="PLQ18_12725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02563.1"
                     /translation="MSAPSGDDGAGRRHPAQLAALLQRQAPTLLLAVLTFVAIGLLSG
                     ATLWQIDNDRVRTLEDARRANVQLADALAEHGSRLIESVDVLLRDFSESPPAPGTGAL
                     GGDFREAYAQSLLFLRNLALLDADGRMYATLYYSEMRGRSFAGRDYYLLHRAQADAGL
                     VVSTPKVSPADGRSRILTLSRRLNDAGGAFAGVVVASVGLDTLERYYRSIERAPGQGI
                     ELRLRDGEAMLSVGVEHNAGPDALEALRWLAGTPYGIVVSWLPVRVLADWRERTGSML
                     VAVAAADTLIAMLALLLGGRLHIGARLLGRLDDAERWQRTLLRTLPDPLVVLDRRLRV
                     REVCSAQPQPFGADIELRPGSALFEVLPPVVAT"
     gene            complement(1112..>1774)
                     /locus_tag="PLQ18_12730"
     CDS             complement(1112..>1774)
                     /locus_tag="PLQ18_12730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02564.1"
                     /translation="SGCTELPGGWRVERWQAPALVDHARPGQRLAAGSAELPVMRTRA
                     ATGELECLARGPAGAAVAAADADLRGEPFELSAPSPRALLIGDLSGLAPLVFLADRLR
                     SAAVRVKTFAILGLDGEAPFRPVPSRLIVPGVPAWVTGTLPLFEDWGIAARLASAGED
                     RPGCFEGTPVQLARGWLAAQQGVRDVCVYACAGPALLEDTRALAAAFGLGYQGRAAGS
                     AC"
BASE COUNT          252 a          651 c          679 g          192 t
ORIGIN      
        1 ggtggcgacg acaggcggca gcacctcgaa cagcgcgctg ccggggcgca gctcgatgtc
       61 ggcgccgaag ggctgcggct gggcgctgca gacctcgcgc acgcgcaggc gccgatcgag
      121 cacgaccagc gggtcgggca gcgtgcgcag cagcgtgcgc tgccagcgct cggcgtcgtc
      181 gagccgcccg agcaggcggg cgccgatgtg caggcgcccg ccgagcagca gcgccagcat
      241 cgcgatcagc gtgtcggcgg cggccacggc gaccagcatg ctgccggtgc gctcgcgcca
      301 gtcggcgagc acccgcaccg gcagccagct gaccacgatc ccgtacggcg tgccggcgag
      361 ccagcgcaag gcttccagcg cgtcggggcc ggcattgtgc tcgacgccga cgctcagcat
      421 cgcctcgccg tcgcgcaggc gcagttcgat gccctggccg ggcgcgcgct cgatcgaccg
      481 gtaatagcgc tccagcgtgt cgagcccgac cgaggcgacc acgacgccgg cgaaggcccc
      541 gccggcatcg ttgagccggc gcgacagcgt caggatgcgc gagcggccgt cggccggcga
      601 caccttcggc gtcgacacca ccagcccggc atcggcctgg gcgcggtgca gcaggtagta
      661 gtcgcgcccg gcgaagctgc gcccgcgcat ctcgctgtag tacagcgtcg cgtacatgcg
      721 gccgtcggcg tcgagcaggg cgaggttgcg caggaacagc aggctctggg cgtaggcctc
      781 gcggaaatcg ccgccaagcg cgccggtgcc gggcgctggc ggggattcgg agaaatcgcg
      841 cagcagcacg tcgacggact cgatcagccg cgaaccgtgc tcggcgagcg catcggcgag
      901 ctggacgttg gcgcggcgcg cgtcctcgag cgtgcgcacc cggtcattgt cgatctgcca
      961 gagcgtcgcc cccgacagca ggccgatcgc gacgaaggtc agcaccgcca gcagcagcgt
     1021 cggtgcctgg cgctgcagca gcgcggcgag ttgcgccggg tggcgccgcc ctgccccgtc
     1081 gtcgccggag ggggcgctca tcggatgccg gtcagcacgc gctgccggca gcgcgtccct
     1141 gataaccgag accgaacgcg gctgccagcg cgcgggtgtc ttcgagcagc gccgggccgg
     1201 cgcaggcata cacgcagaca tcgcgcacgc cctgctgcgc tgcgagccag ccgcgtgcga
     1261 gctgcaccgg cgtgccttcg aagcagccgg gccggtcctc gccggcgctg gcgaggcgcg
     1321 cggcgatgcc ccagtcctcg aacagcggca gcgtgccggt cacccaggcc ggcacgccgg
     1381 gcacgatcag gcgcgagggc accgggcgga acggtgcctc gccgtcgagc ccgaggatcg
     1441 cgaaggtctt gacgcgcacc gcggcgctgc gcagccggtc ggcgaggaac accagcggcg
     1501 cgagcccgga cagatcgccg atcagcagcg cgcgcgggct cggcgccgac agctcgaagg
     1561 gctcgccgcg caggtcggca tcggccgcgg cgaccgcggc gccggccggg cctcgtgcga
     1621 ggcattcgag ttcgccggtc gccgcgcgcg tgcgcatgac cggcagctcg gcgctgccgg
     1681 cggccagccg ctggccggga cgggcatgat cgaccagcgc cggggcctgc cagcgctcga
     1741 cgcgccagcc accgggcagt tcggtacatc cgga
//
LOCUS       DAOQJH010000642         1773 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_58312_length_1773_cov_4.245052, whole genome shotgun sequence.
ACCESSION   DAOQJH010000642 DAOQJH010000000
VERSION     DAOQJH010000642.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1773
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58312_length_1773_cov_4.245052"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..846)
                     /locus_tag="PLQ18_12735"
     CDS             complement(<1..846)
                     /locus_tag="PLQ18_12735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HNE02565.1"
                     /translation="MPSIRLWRAVALAAALLAVAAVAPAAQFINVLTGGTSGVYYPLG
                     VALAKIFETAVPDSKPSVQATKASVENLNLLQAGRGELAFTLGDSLSQAWNGNEEAGF
                     RAPLKKLRTVAAIYPNYIQIVAAKESGIRTLADLKGRRISVGAPKSGTELNARAVLGA
                     AGLKYEDFDKVEYLPFGESVELMKNRQLDATLQSAGLGVASIRDLAQAVPIVVVAVPA
                     ETVGKIGDAAYRAAVIPANTYEGQTEDVPAVAVVNFLVTHEGVPEEVVYAMTKGMFEH
                     LDALAA"
     gene            complement(989..>1773)
                     /locus_tag="PLQ18_12740"
     CDS             complement(989..>1773)
                     /locus_tag="PLQ18_12740"
                     /inference="COORDINATES: protein motif:HMM:NF023227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HNE02566.1"
                     /translation="LDVSGVGVCRGSATLIARDLKIAVRAATGLVASAGVSYNKFLAK
                     IASDLDKPDGLAVITPEQGPAFIAKLPIGRFHGIGRATEQKMLALGIATGADLGAWSE
                     ARLVEHFGKAGHWYAQLARGEDDRPVCAQRVRKSLGAETTYARDLGAAGEIEAALGAL
                     ADEVATELGRRGLAGRTLTLKVRYADFETVTRAHTLAPPARYAQAAELRTALPALLAR
                     TEACSRPVRLLGLTVSGFPEEDGGEAESRGAAIGRMVQLPLF"
BASE COUNT          251 a          627 c          636 g          259 t
ORIGIN      
        1 ggcggcgagc gcgtcgaggt gctcgaacat gcctttggtc atcgcgtaga cgacctcctc
       61 cggcacgccc tcgtgcgtga ccaggaagtt cacgaccgcc accgccggca cgtcttcggt
      121 ctggccctcg taggtgttgg ccgggatcac ggcggcgcgg taggcggcat cgccgatctt
      181 gccgaccgtt tcggccggca ccgcgacgac gacgatcggc acggcctgcg cgaggtcacg
      241 gatcgacgcg acgccgaggc cggccgactg cagcgtcgcg tcgagctggc ggttcttcat
      301 cagctcgacc gactcgccga agggcaggta ctcgaccttg tcgaagtcct catatttcag
      361 gccggcggcg ccgagcacgg cgcgggcgtt gagttcggtg ccggacttcg gcgcgccgac
      421 cgagatgcgc ctgcccttga gatcggcgag cgtgcggatg ccggattcct tcgcggcgac
      481 gatctggatg tagttcgggt aaatcgccgc gaccgtgcgc agcttcttca gcggcgcgcg
      541 aaagcccgcc tcctcgttgc cgttccaggc ctggctcagc gaatcgccga gcgtgaacgc
      601 cagctcgccg cgcccggcct gcagcaggtt caggttctcg accgaagcct tggtcgcctg
      661 caccgagggt ttcgaatcgg gcacggcggt ctcgaagatc ttcgcgagtg cgacgccgag
      721 cgggtaatag acgccgctgg tgccaccggt cagcacgttg atgaactgcg cggccggcgc
      781 gacggcggcg acggcgagca gggcggcggc gagcgcgacg gcgcgccaca ggcggatcga
      841 cggcatgggg attctcctcg ggtcgggtgc ctctggcctg cggcaggcgc gggccgggca
      901 acttgtcatt gatgcttgcc ccggcagata tagcccagcc cgccgcgccg gggccggccg
      961 atccacagcc ggtccgcacc ggtgcgcttc agaacagcgg cagctgcacc atgcgcccga
     1021 tcgcggcacc gcgggattcc gcttcgccgc cgtcctcttc cgggaagccc gagacggtca
     1081 gcccgagcag gcgcaccggc cgcgagcacg cctcggtgcg cgccaagagg gccggcagcg
     1141 ccgtgcgcag ctcggcggcc tgcgcatagc gcgccggcgg cgccagcgtg tgggcgcggg
     1201 tgacggtctc gaagtcggcg tagcgcacct tcagcgtcag cgtgcgcccg gcgagaccgc
     1261 ggcggccgag ttcggtcgcg acctcatcgg cgagggcgcc gagcgcggcc tcgatctcgc
     1321 cggcggcgcc gagatcgcgc gcataggtgg tctcggcccc gagcgacttg cgcacgcgct
     1381 gcgcacacac cggccggtcg tcctcgccgc gcgcgagctg cgcgtaccag tgcccggcct
     1441 tgccgaagtg ctcgacgagg cgcgcctcgg accacgcccc gagatcggcg ccggtggcga
     1501 tgccgagcgc gagcatcttc tgctcggtgg cgcggccgat gccgtgaaag cgcccgatcg
     1561 gcagcttcgc gatgaacgcc ggcccctgct ccggcgtgat caccgccagg ccgtcgggct
     1621 tgtcgagatc ggaggcgatc ttggcgagga acttgttgta cgagacgccg gccgaggcca
     1681 ccagcccggt cgcggcacgc acggcgatct tcaggtcccg cgcgatcagc gtcgccgatc
     1741 cacgacatac acccacaccg ctgacatcga gat
//
LOCUS       DAOQJH010000643         1758 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_59349_length_1758_cov_4.898415, whole genome shotgun sequence.
ACCESSION   DAOQJH010000643 DAOQJH010000000
VERSION     DAOQJH010000643.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1758)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1758)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1758
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59349_length_1758_cov_4.898415"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..810)
                     /locus_tag="PLQ18_12745"
     CDS             complement(<1..810)
                     /locus_tag="PLQ18_12745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017246318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HNE02567.1"
                     /translation="MSLFSAVEMAPRDPILGLNEAYNADPRATKVNLGVGVYFTDAGR
                     IPLLRAVQEAEKARIAAAAPRGYLPIEGIAAYDKLVQELLFGTASPLLAEGRVVTAQA
                     LGGTGALKIGADFLRRLLPGASVAISDPSWENHRALFESAGFEVGSYRYYDAPNHGVD
                     LAGMLADLQALPANSIVVLHACCHNPTGADLTPAQWQQVLAVLAERGHVPFLDIAYQG
                     FGDGIDADAVAVRLFAESGLPFFVASSFSKSFSLYGERVGALSIVTGSRDES"
     gene            942..>1758
                     /locus_tag="PLQ18_12750"
     CDS             942..>1758
                     /locus_tag="PLQ18_12750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HNE02568.1"
                     /translation="MQRFELVTDYQPAGDQPAAIDALVRGLEDGLAHQTLLGVTGSGK
                     TFTMASVIARVQRPAMILAQNKTLAAQLYGEMKAFFPHNAVEYFVSYYDYYQPEAYVP
                     ASDTFIEKDASINAHIEQMRLSATKAILERADTIIVASVSSIYGLGDPELYLQMILHL
                     SRGERIDQRDILKRLTALQYVRNDAVLERGSFRVRGEVIDVHPADSETEAVRIELFDD
                     EIEQLSYFDPLTGVVARRVPRLTIHPKTHYVTPRARILETVDAVKLELAARVTE"
BASE COUNT          304 a          591 c          613 g          250 t
ORIGIN      
        1 cgattcgtcg cggctgccgg tgacgatcga cagcgcgccg acacgctcgc cgtacagcga
       61 gaacgacttc gagaacgagc tggcgacgaa gaacggcagg ccggattcgg cgaacaggcg
      121 caccgcgacc gcgtcggcat cgatgccgtc gccgaagccc tggtaggcga tgtcgaggaa
      181 gggcacgtga ccgcgctcgg cgagcacggc cagcacctgc tgccactgcg cgggcgtcag
      241 gtcggcgccg gtcgggttgt ggcagcaggc gtgcagcacg acgatcgagt tcgccggcag
      301 ggcctggagg tcggcaagca tgccggcgag gtcgacgccg tggttcgggg cgtcgtaata
      361 gcggtagctg ccgacctcga agccggccga ttcgaacagc gcgcggtggt tttcccagct
      421 cggatcggag atcgcgacgc tggcgccggg cagcaggcgg cgcaggaaat cggcgccgat
      481 cttcagcgcg ccggtgccgc cgagcgcctg cgcggtgacg acgcggcctt cggcgagcag
      541 cggtgaagcg gtgccgaaca gcagttcctg caccagcttg tcgtaggcgg cgatgccttc
      601 gatcggcagg tagccgcgcg gcgcggccgc ggcgatgcgg gccttctcgg cctcctggac
      661 ggcgcgcagc agcgggatgc ggccggcgtc ggtgaagtag acgcccacgc ctagattgac
      721 cttggtggcg cgcggatcgg cgttgtaggc ctcgttgagc ccgaggatcg ggtcgcgcgg
      781 ggccatttcg acggcagaga acaggctcat gtcggcagct cggagctaac agggagaaag
      841 ggcgaagaac cggaggatca acggcttgtc ttgaacgggc cgcagcgggc cagtatatcc
      901 ccttccccat gcgccgcgaa gcaggccgag gagccacgag catgcagcga ttcgaactcg
      961 tcaccgacta tcagccggcc ggcgaccagc cggcggcgat cgacgcgctg gtgcgcggcc
     1021 tggaggacgg gctcgcgcac cagacgctgc tcggcgtcac cggcagcggc aagaccttca
     1081 cgatggccag cgtcatcgcc cgcgtgcagc gtccggcgat gatcctcgcg cagaacaaga
     1141 cgctggccgc gcagctctac ggcgagatga aggcgttctt cccgcacaac gccgtcgagt
     1201 acttcgtctc gtactacgac tactaccagc ccgaggccta cgtgccggcc tcggacacct
     1261 tcatcgagaa ggacgcctcg atcaacgccc acatcgagca gatgcggctg tcggcgacca
     1321 aggcgatcct ggagcgcgcc gacacgatca tcgtcgcgtc ggtgtcctcg atctacggcc
     1381 tcggcgaccc cgagctgtac ctgcagatga tcctgcacct gtcgcgcggc gagcgcatcg
     1441 accagcgcga catcctcaag cgcctcacgg cgctgcagta cgtgcgcaac gacgccgtgc
     1501 tcgagcgtgg cagcttccgc gtgcgcggcg aggtcatcga cgtgcacccg gccgattccg
     1561 aaaccgaggc cgtgcgcatc gagctgttcg acgacgagat cgagcagctg tcgtacttcg
     1621 atccgctgac cggcgtcgtc gcgcggcgcg tgccgcgcct gaccatccac ccgaagacgc
     1681 attacgtgac gccgcgcgcg cgcatcctcg aaaccgtcga tgccgtgaag ctcgaactcg
     1741 ccgcgcgcgt caccgaac
//
LOCUS       DAOQJH010000644         1755 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_59529_length_1755_cov_3.164118, whole genome shotgun sequence.
ACCESSION   DAOQJH010000644 DAOQJH010000000
VERSION     DAOQJH010000644.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1755)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1755)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1755
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59529_length_1755_cov_3.164118"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..267
                     /locus_tag="PLQ18_12755"
     CDS             <1..267
                     /locus_tag="PLQ18_12755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02569.1"
                     /translation="VDPAEVRKDRMLRTRFMSFFPDFMTLAEQMEDTMTILGNEILMA
                     DLAYYQSVREAARRGVPGARQIYDDLRTRFPGGGARAAAPEKTA"
     gene            complement(1094..>1755)
                     /locus_tag="PLQ18_12760"
     CDS             complement(1094..>1755)
                     /locus_tag="PLQ18_12760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017938575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNE02570.1"
                     /translation="GVHLIADEIAVGFGRTGTLFACEQAGITPDFLCLSKGLTGGYLP
                     MSVVLTGDDIYRAFYDDYGKLTAFLHSHSYTGNPLAARAALATLDLFAASDVLGRNRE
                     LAAHMARLAGPFRTHPHIGDVRQRGMILAFEMVAAPATRTPYPWQQRRGLDVYRHALD
                     RGALLRPLGNVVYFMPPYVITEAELDRLAEIAWECIELVTLDPLPFGDIEPDGRSGWL
                     A"
BASE COUNT          269 a          621 c          612 g          253 t
ORIGIN      
        1 gtcgaccccg cggaagtccg caaggaccgc atgctgcgca cgcgcttcat gagcttcttc
       61 ccggatttca tgacgctcgc cgagcagatg gaagacacga tgaccatcct cggcaatgaa
      121 atcctgatgg ccgatctcgc ctattaccag agcgtgcgcg aggccgcccg ccgcggcgtg
      181 ccgggagccc ggcagattta cgacgatctg cgcacccgct tccccggcgg cggcgcccgg
      241 gccgcggccc ccgagaagac cgcctgaccc cctcgccacc ccggcggcgg gtccgttaca
      301 cccgccgccg cccttgcagc tgtcggacgc gccagccgat ccattgcggt cgagcccctc
      361 gatcccggcg ccgctgctgc gaatcccggc accgataccc gtcgtatcgg cgccggtatc
      421 cgaactgccg gcatcgatgc cggcatcacc gccgccgcga tcccgacggc cggcgccggt
      481 gccgccagtc ccgcgccggc ttgcggaaac cccgccgtcg aggtccggca cctcggcaac
      541 gaaggtctgc acatcccggt caggggtccg cacccctgcg ccgccccttt ttgcagcgcc
      601 gccgcccttt tttaccgctg cgccggttgt tcaggtatcg acgccgacac ctgagcaggg
      661 cgggggactg ctgaagtacc ggcgccgaca cttcaggtat cgaagccgat atctgaacca
      721 ccggcgccgg tgttccacat accggcgccg atgccggggc atcgacgccg atacttcaac
      781 tatcggcgtc gactcattct ggggcgacgg cgccccagcg cggggtgaag tcgggacttc
      841 gggagagggc ggcgacagcg acgctcaggc gcaggggcgt gtccctgccc gcttgcgcac
      901 ggcgaatcgt gccgccgcac gcgggccggg cggggcctgc gcgacagcat tcgcagagaa
      961 aagtcagggg cttacggcgc tggtcgcggt gcgtgggcgg gcttgaggcg cacaagccgg
     1021 catacgggaa gtggcgttca ggcgccgtgc gagcccgcgc cggggccatc gacgaccgcc
     1081 ggccatgcgg ccgtcacgcc agccagccgc tgcgcccgtc gggttcgatg tcgccgaagg
     1141 gcagcggatc gagcgtcacc agttcgatgc actcccacgc gatctcggcg agccggtcga
     1201 gttcggcttc ggtgatcacg tacggcggca tgaagtagac gacgttgccg agcgggcgca
     1261 gcagtgcgcc gcgatcgagc gcatgccggt agacatcgag gccgcggcgc tgctgccacg
     1321 gatacggcgt gcgcgtggcc ggggcggcga ccatctcgaa cgcgaggatc atgccgcgct
     1381 ggcgcacgtc gccgatgtgc ggatgcgtgc ggaacggccc ggcaagcctt gccatgtgcg
     1441 cggccagttc gcggttgcgg ccgagcacgt cgctcgcggc gaacagatcg agcgttgcca
     1501 gcgccgcgcg cgccgccagc ggattgccgg tgtagctgtg cgagtgcagg aaggcggtca
     1561 gcttgccgta gtcgtcgtag aaggcgcggt agatgtcatc gccggtcagc accaccgaca
     1621 tcggcaggta gccgccggtc aggcccttcg acaggcacag gaaatccggc gtgatgccgg
     1681 cctgctcgca cgcgaacagc gtgccggtgc ggccgaagcc gaccgcgatc tcgtcggcga
     1741 tcaggtgcac gccgt
//
LOCUS       DAOQJH010000645         1754 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_59590_length_1754_cov_3.228958, whole genome shotgun sequence.
ACCESSION   DAOQJH010000645 DAOQJH010000000
VERSION     DAOQJH010000645.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1754)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1754)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1754
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59590_length_1754_cov_3.228958"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            267..>1754
                     /locus_tag="PLQ18_12765"
     CDS             267..>1754
                     /locus_tag="PLQ18_12765"
                     /inference="COORDINATES: protein motif:HMM:NF012876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HNE02571.1"
                     /translation="MSIRSRLLALILVLSALLLALTVNDGFDARRRSEQAAEAWRHNA
                     AAALLANATTNWARERGATNGALNAADAITAQAREAIRRYREQADTAAGQALAALRNE
                     TLEAREQKLLADADAAWSRLRTLRERVDRDLPRPRAERDAALLKDWLPGITASVEATE
                     RLSLEIDRQPESASMRLARLQALRHFAWQMSEYMGRERAVIAGIIAAGKPMLSSDLER
                     LGRMRGRVELAWERVAQLASQGDNDAGLVQAVATVRESVFERFEQTRHQVIEAGTLGW
                     DYPFPAGEWFARATQAIDTIIALVDAVGEEAGNQAGSARDTAASSASGFIALAAFCLA
                     LIALAIWVVMSGIVAPLRRLTATTTALAGGALDTPVPDTGRRNEIGDLARAVEVLRGN
                     SLEAQRLRAEQDRARTEAEFERARRAREDEALRTEREAEKERMRAAEEAVRAQREAEK
                     EQERQRQLLRSERITQLTTHFGREAAGMLDAVTSAADGLERTAERM"
BASE COUNT          269 a          651 c          612 g          222 t
ORIGIN      
        1 tagctcgtcg ggctcataac ccgaaggtcg taggttcaaa tcctgccccc gctaccaaat
       61 ccgaaggccc ggtaccgaac atcggtaccg ggccttcgcc tttgtgcgtc cgtgcgggtc
      121 tgcccttgcg tctgccccgc acgcgaccag acttggcact cccccagggt ccgctgcact
      181 cctcccgacg tccggccgcc ctgctcaagc agcggtccgt gccggccgat acgtggttct
      241 gctgaaattt gccggagtgc aagccgatgt cgatccgcag ccgactgctc gccctgatcc
      301 tcgtcctgtc cgcgctgttg ctggcgctga ccgtcaacga cggattcgat gcccgccgtc
      361 gcagcgagca ggccgccgaa gcctggcggc acaacgccgc cgccgcactg ctcgccaatg
      421 cgacaaccaa ctgggcccgg gaacgcggcg cgaccaacgg cgccctgaac gctgccgatg
      481 ccatcaccgc gcaggcacgc gaggcgatcc ggcgctaccg cgagcaggcc gacaccgccg
      541 cgggccaggc gctggcggcg ctgcgtaacg aaaccctgga agcccgggag cagaagctgc
      601 tcgccgatgc cgacgccgcc tggagccggc tcaggacgct gcgcgagcgg gtcgaccgcg
      661 acctgccccg cccgcgcgcc gagcgcgatg cggcgctgct gaaggactgg ctgcccggca
      721 tcaccgccag cgtcgaggcc accgaacgcc tgtcgctgga gatcgaccgg cagccggaat
      781 cggcgtcgat gcgcctcgcg cgcctgcagg cgctgcggca cttcgcgtgg cagatgagcg
      841 aatacatggg gcgcgagcgc gccgtcatcg ccggcatcat cgccgccggc aagccgatgc
      901 tgtcgtccga tctcgaacgg ctcggccgca tgcgcgggcg cgtcgaactg gcctgggagc
      961 gcgtcgcaca gctcgccagc cagggcgaca acgacgccgg cctcgtgcag gcggtcgcga
     1021 cggtgcgcga gtcggtgttc gagcgtttcg agcagacccg ccatcaggtc atcgaggccg
     1081 gcacgctcgg ctgggactat ccgttcccgg ccggcgagtg gtttgcgcgc gccacacagg
     1141 cgatcgacac catcatcgcg ctggtcgatg ccgtcggtga ggaagccggc aaccaggcgg
     1201 gcagcgcgcg cgacaccgcc gcgagcagcg ccagcggttt catcgcgctg gccgccttct
     1261 gcctggcgct gatcgcgctg gcgatctggg tggtcatgtc cggcatcgtc gcgccgctgc
     1321 gccgcctgac cgcgaccacg accgcgctcg ccggcggtgc gctcgatacg ccggtgccgg
     1381 acaccggccg acgcaacgag atcggcgatc tggcacgcgc ggtcgaggtg ctgcgcggca
     1441 actcgctgga agcgcagcgc ctgcgcgccg agcaggaccg cgcccgcacc gaggccgagt
     1501 tcgaacgcgc ccgccgcgcg cgcgaggacg aggcgctgcg caccgagcgc gaggcggaaa
     1561 aggaacgcat gcgcgccgcc gaggaagccg tgcgcgcgca gcgcgaggcc gagaaggagc
     1621 aggagcgcca gcgccagctg ctgcgcagcg agcgcatcac gcagctcacc acccacttcg
     1681 gccgcgaggc cgccggcatg ctcgatgccg tgacctcggc cgcggacggg ctggagcgca
     1741 ccgccgagcg catg
//
LOCUS       DAOQJH010000646         1743 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_60257_length_1743_cov_3.994668, whole genome shotgun sequence.
ACCESSION   DAOQJH010000646 DAOQJH010000000
VERSION     DAOQJH010000646.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1743
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60257_length_1743_cov_3.994668"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..439
                     /locus_tag="PLQ18_12770"
     CDS             <1..439
                     /locus_tag="PLQ18_12770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02572.1"
                     /translation="VVDAELGRKYYRLLGVRAEEPAMREALKVYYPATILRDSYAWLG
                     TDVQTLAVKAYLITYDYPQDFNRRAIAAFARSLCDNYATLRAQGHPKWQQVAWSPQQP
                     ALEPLKAGWQYYSVTEPILSGCSGGGNGNAACSSDRRLLGLCR"
     gene            complement(486..1430)
                     /locus_tag="PLQ18_12775"
     CDS             complement(486..1430)
                     /locus_tag="PLQ18_12775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02573.1"
                     /translation="MPRLLASLVLALGLSLPAAAADLAKLRAAFASVRFIAYSPSAYT
                     DGGLVGRDAIRRDLLILAPDFDGIVTYASDNGSEQVAALARETGIRAVILGVHNPANT
                     TELDTAIRLAREYPEVVVAICVGNEGLISHRYDWPTLRRGLDHVRAALPELPLTTGEP
                     FVIHLEWTPPGFFDAQDFIGVNIHPLFEGWFTPDKLDAGMDYLDAILGKVIARAPGKP
                     VMIKETGVPGQPGGEWSPAVQAGFWTRVERRFPRNGDRAFSAFEAFDAPWKPALIARD
                     FKDYGGTVDPREAYWGFYTVDGEPKPVVEQLRAQRRGP"
     gene            complement(1522..1716)
                     /locus_tag="PLQ18_12780"
     CDS             complement(1522..1716)
                     /locus_tag="PLQ18_12780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014993512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02574.1"
                     /translation="MADILPFKRPKPADRMEAAAGRELCKSGFHKWEVQKDKRFDVKA
                     GRLVTSYRCARCGATRTEGR"
BASE COUNT          256 a          597 c          637 g          253 t
ORIGIN      
        1 cgtcgtcgat gccgaactcg ggcgcaagta ctaccggctg ctcggcgtgc gcgccgagga
       61 accggcgatg cgcgaggcgc tgaaggtcta ctacccggcg acgatcctgc gcgacagcta
      121 cgcatggctc ggcaccgacg tgcagacgct cgcggtcaag gcctacctga tcacctacga
      181 ctatccgcag gacttcaacc gccgcgcgat cgcggccttc gcgcgctcgc tgtgcgacaa
      241 ctacgccacg ctgcgcgcgc agggccatcc gaagtggcag caggtggcct ggagtccgca
      301 gcagccggcg ctggaaccgc tgaaggccgg ctggcagtac tactcggtga ccgaaccgat
      361 cctgtcgggc tgcagcggcg gcggcaatgg caatgcggcg tgcagcagcg accggcggct
      421 gctcgggctg tgccgctgac cccgccggcg gcggggcggc gtggggaggc ttcctgcttc
      481 gggcctcacg gcccccgccg ctgcgcccgc agctgttcga cgaccggctt cggttcgccg
      541 tcgacggtat agaagcccca gtacgcctcg cgcggatcga ccgtgccgcc gtagtccttg
      601 aagtcgcgcg cgatcagcgc cggcttccac ggcgcatcga aggcttcgaa ggccgagaac
      661 gcacggtcgc cgttccgcgg aaagcgtcgc tcgacccgcg tccagaagcc ggcctgcacc
      721 gccggcgacc attcgccgcc gggctgaccg ggcacgccgg tctccttgat catcaccggc
      781 ttgcccggcg cgcgcgcgat gaccttgccg aggatcgcgt cgagatagtc catgccggca
      841 tcgagcttgt cgggcgtgaa ccagccctcg aacagcggat ggatgttgac gccgatgaag
      901 tcctgcgcgt cgaagaagcc cggcggcgtc cattcgaggt ggatcacgaa cggctcgccg
      961 gtggtcagcg gcagttcggg cagcgccgcg cgcacgtgat cgaggccgcg ccggagcgtc
     1021 ggccagtcgt agcggtgcga gatcagccct tcgttgccga cgcagatcgc gaccacgact
     1081 tcggggtatt cgcgcgccag ccggatcgcg gtgtcgagtt cggtggtgtt ggcggggttg
     1141 tggacgccga ggatcacggc gcggatgccg gtttcgcgtg caagcgccgc gacctgctcg
     1201 ctgccgttgt cgcttgcata ggtgacgatg ccatcgaaat ccggcgccag gatcaggaga
     1261 tcgcgccgga tcgcatcccg gcccaccagc ccgccgtcgg tgtaggcgct cgggctgtag
     1321 gcgatgaagc gcaccgacgc gaacgccgcg cgcagcttcg cgagatcggc ggccgcagcc
     1381 ggcagcgaca ggccgagggc gaggacgagg cttgcaagca ggcggggcat ggcggaaacc
     1441 gggggtggca gggggccggc cagcatacgc gccgccggcg ccggccgatg cagcggccgg
     1501 gcgttgcggc cggcgcgccg gctagcggcc ttcggtccgg gtcgcgccgc agcgtgcgca
     1561 gcggtagctc gtgaccagcc tgccggcctt cacgtcgaag cgcttgtcct tctgcacctc
     1621 ccacttgtga aacccggact tgcaaagctc ccggccggcc gcggcctcca tgcggtcggc
     1681 aggcttgggg cgcttgaacg gcaggatgtc ggccatgcgg gcggacctcg gcgggcggat
     1741 cgg
//
LOCUS       DAOQJH010000647         1742 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_60341_length_1742_cov_3.193835, whole genome shotgun sequence.
ACCESSION   DAOQJH010000647 DAOQJH010000000
VERSION     DAOQJH010000647.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1742)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1742)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1742
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60341_length_1742_cov_3.193835"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(123..908)
                     /locus_tag="PLQ18_12785"
     CDS             complement(123..908)
                     /locus_tag="PLQ18_12785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013593531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase family protein"
                     /protein_id="HNE02575.1"
                     /translation="MPRFAANLSFLFTELPFPERFAAAAAAGFGGVEFLFPYAWPVEA
                     IAGWRRAASVDSVLFNLPPGDWEAGERGLACLPGREAEFLDGIERALPYALALGTPRL
                     HAMAGLLPTGADPAHCRQVYVDNLRRAAARLAAHGIMLLIEPINPRDMPGYFLNTQAD
                     AHAIREAVGAPNLRVQTDLYHAQIVEGDLAVKLERWLAHIGHLQIAGVPGRHEPDHGE
                     IHYPYLFALLDRLGYDGWIGCEYRPAAGTVEGLGWYAPWRTQR"
     gene            complement(959..1408)
                     /locus_tag="PLQ18_12790"
     CDS             complement(959..1408)
                     /locus_tag="PLQ18_12790"
                     /inference="COORDINATES: protein motif:HMM:NF024901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HNE02576.1"
                     /translation="MLLTPSRCARCWLAVLLALAWPAMAGVYRWRDADGRMHYGDQPP
                     AGVQAEALPVDPAPPPRSAADAERRLLEDADRRAADTARGRSEARRREARAEAERERR
                     CSQLDERRRQLETGFAERRARGYTMAQERSYRSRLDALERELKSGCR"
     gene            1558..>1742
                     /locus_tag="PLQ18_12795"
     CDS             1558..>1742
                     /locus_tag="PLQ18_12795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007013408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HNE02577.1"
                     /translation="MARLILFNKPYDVLSQFTDREHGRTTLADFIDLPGVHVAGRLDR
                     DSEGLLLLTDDGRLQAR"
BASE COUNT          236 a          611 c          673 g          222 t
ORIGIN      
        1 gacggcgagt gcgagcgcga gcagcgccgg caccgctgag gccagcaagc gctgccagcg
       61 gcgatagccg gccggtccgg gcgcgtgcgg ggcaagcggc gcggcggcgc ccggcggcgg
      121 attcagcgct gcgtccgcca cggcgcgtac cagccgagcc cctcgacggt gccggccgcc
      181 ggacggtatt cgcagccgat ccagccgtcg tagccgagcc ggtcgagcag cgcgaacagg
      241 taggggtagt ggatctcgcc gtggtcgggc tcgtggcgcc cgggcacgcc ggcgatctgc
      301 aggtggccga tgtgcgccag ccagcgttcg agcttcacgg ccagatcgcc ctcgacgatc
      361 tgcgcgtggt acaggtccgt ctgcacccgc aggttcggcg cgccgaccgc ctcgcggatc
      421 gcgtgcgcgt cggcctgcgt gttcaggaag tagccgggca tgtcgcgcgg attgatcggt
      481 tcgatcagca gcatgatgcc gtgtgcggcc agccgtgcgg cggcgcggcg caggttgtcg
      541 acatagacct gccggcagtg cgccggatcg gccccggttg gcagcagccc ggccatcgcg
      601 tgcagccggg gcgtgccgag ggcgagcgca tacggcagcg cgcgctcgat cccgtccaga
      661 aactcggcct cgcgccccgg cagacaggcg aggccgcgtt cgccggcctc ccagtcgccg
      721 ggcggcaggt tgaacagcac gctgtcgacg ctggcggcgc gccgccagcc ggcgatggcc
      781 tcgacgggcc aggcatacgg aaacaggaac tcgaccccgc cgaagccggc ggcggccgcg
      841 gcggcgaagc gttccggaaa cggcagctcg gtgaacagga agctcaggtt cgcggcaaag
      901 cgcggcatgg cgactccgca ggcggcatgg caggaagaac ggggggcagg ggccggtgtc
      961 agcgacagcc ggatttgagc tcgcgttcca gcgcatcgag ccggctccgg taactgcgct
     1021 cctgcgccat cgtgtagccg cgcgcgcggc gctcggcgaa tccggtttcg agctggcggc
     1081 ggcgttcgtc gagctggctg cagcggcgct cgcgctcggc ctcggcgcgc gcttcgcggc
     1141 ggcgggcctc gctgcgcccg cgcgccgtgt cggctgcacg ccggtcggcg tcctccagca
     1201 gccggcgctc ggcatcggcc gcactgcgcg gcggcggggc gggatcgacc ggcagggctt
     1261 cggcctgcac gccggccggc ggctggtcgc cgtagtgcat gcggccgtcg gcatcgcgcc
     1321 agcggtagac cccggccatg gccggccagg cgagggcgag cagcaccgcc agccagcagc
     1381 gggcgcagcg ggatggcgtc agcagcatcg ggacattccc gtcctgcccg ggccggtcgc
     1441 acggggtctt gcgtcagcgg gccggtatgc tgcacggcgt gaccgcccgg ctgcacgaag
     1501 acccacaggc ctgcgccagg gcggttgtgg cggatacgac gaacccggcg gcggacgatg
     1561 gcgagactga tcctgttcaa caagccctat gacgtgctct cgcagttcac cgaccgcgag
     1621 cacgggcgca cgacgctcgc ggatttcatc gatctgcccg gcgtccacgt cgccggccgg
     1681 ctcgatcgcg acagtgaggg cctgctgctg ctgaccgacg acggccggct gcaggcgcgc
     1741 at
//
LOCUS       DAOQJH010000648         1737 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_60686_length_1737_cov_5.256243, whole genome shotgun sequence.
ACCESSION   DAOQJH010000648 DAOQJH010000000
VERSION     DAOQJH010000648.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1737
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60686_length_1737_cov_5.256243"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..513
                     /locus_tag="PLQ18_12800"
     CDS             <1..513
                     /locus_tag="PLQ18_12800"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015280882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNE02578.1"
                     /translation="GRLTEQKDFPTLLRAFATVRAARPARLAILGEGRGRAALTALAQ
                     ELGIADALLLPGFAPNPYAWMARARLFVLSSAWEGSPNVLTEALALGVPSVATDCPSG
                     PREVLAGGRFGPLVPVGDHAQLAQAMLATLAAPLAPETLRAAVADYTQAAAAAAYLRV
                     LLPQRQPERP"
     gene            640..1299
                     /locus_tag="PLQ18_12805"
     CDS             640..1299
                     /locus_tag="PLQ18_12805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012971286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase family 2 domain-containing
                     protein"
                     /protein_id="HNE02579.1"
                     /translation="MLLSLKYNFLFVHTAKTGGTSVRDALRPLRLRDPWFYAQFLCSR
                     LSGLSGHRLGIKFPRHSKIIAAQEMLPHELFEKLFKFVFVRNPWDLQVSSFHHIRRER
                     PHLMAHVETFEQFMRWKLDPERPYQYHVDTSIELQSDYCIDLHGKVLVDFIGRTENLQ
                     PDFEEACRRIGIQPPPLPHKRQAKDRKKDYRSYYTDELAELVAERFRPDIELFGYTFE
                     P"
     gene            complement(1376..>1737)
                     /locus_tag="PLQ18_12810"
     CDS             complement(1376..>1737)
                     /locus_tag="PLQ18_12810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02580.1"
                     /translation="MSARTPKPLFPWRKSEPQPLRVDVPPADPTPFQNAEKLVEAVNI
                     AASTVAALHVAFMALSAYLAVTVWGATHVDFLKESPVKLPLIDVQVDLTSFYGVAPWL
                     YVLVHLNLLIRTREPPP"
BASE COUNT          280 a          588 c          594 g          275 t
ORIGIN      
        1 ggccggctga ccgagcagaa ggatttcccg acgctgctgc gcgcgttcgc gacggtgcgc
       61 gccgcgcgcc cggcgcggct cgcgatcctc ggcgaaggcc gcgggcgcgc ggcgctgacg
      121 gcgctggcgc aggaactcgg cattgccgat gcgctgctgc tgccgggctt cgcgccgaat
      181 ccgtatgcgt ggatggcgcg cgcgcggctg ttcgtgctgt cctcggcctg ggagggttcg
      241 ccgaacgtgc tgaccgaggc gctggcgctc ggcgtgccgt cggtcgccac cgactgcccg
      301 agcggtccgc gcgaggtgct ggccggcggg cgcttcgggc cgctggtgcc ggtcggcgat
      361 cacgcgcaac tggcgcaggc gatgctggcg acgctggccg caccgctcgc gcccgagacc
      421 ttgcgcgccg cggtcgccga ctacacgcag gccgcggcgg cggcggcgta tctgcgcgtg
      481 ctgctgccgc aacggcagcc ggagcggccg tgaacgccgg gcgaggcggc ggccgttcaa
      541 accagacgat ctgaaaattc tacttttaat gctttccttt cacgccgggt ccgcaccgac
      601 catggcgcca tcccgatccc cgtgcgcgga ggcgccgaaa tgctgctgtc gctgaagtac
      661 aacttcctgt tcgtacacac cgccaagacc ggcggcacct cggtgcgcga tgcgctgcgg
      721 ccgctgcggc tgcgcgatcc gtggttctac gcgcagttcc tgtgctcgcg gctgtcgggg
      781 ctgagcgggc accggctcgg catcaagttt ccgcggcatt cgaagatcat cgcggcgcag
      841 gagatgctgc cgcacgagct gttcgagaag ctgttcaagt tcgtgttcgt gcgcaatccg
      901 tgggatctgc aggtgagttc cttccaccac atccgccgcg aacgcccgca cctgatggcg
      961 cacgtcgaga ccttcgagca gttcatgcgc tggaagctcg acccggagcg cccgtaccag
     1021 taccacgtcg ataccagcat cgagctgcag tcggattact gcatcgacct gcacggcaag
     1081 gtgctggtgg atttcatcgg ccgcaccgaa aacctgcagc ccgacttcga ggaagcctgc
     1141 cgccgcatcg gcatccagcc gccgccgctg ccgcacaagc gccaggcgaa ggaccgcaag
     1201 aaggattacc ggagttatta caccgacgaa ctggcggagc tggtggcgga gagattccgg
     1261 ccggacatcg agctgttcgg gtatacgttc gagccgtagg agccggcacc gaccggcggc
     1321 gcgacgcgcg gccgggtaac ggacgaacct ccgcacgatt ccggcgctga cacgatcagg
     1381 ggggaggctc acgggtccgg atcagcagat tcagatgcac cagcacgtac agccacggcg
     1441 ccacaccgta gaagctggtc agatcgacct gtacgtcgat cagcggcagc ttgaccgggc
     1501 tttccttcag gaaatcgacg tgcgtcgcgc cccagaccgt caccgccaga tacgcgctga
     1561 gcgccatgaa cgccacatgc agcgccgcca ccgtgctggc ggcaatattg accgcctcga
     1621 ccagcttttc ggcgttctgg aacggcgtcg ggtccgccgg cggcacatcg acccgcaacg
     1681 gctgtggctc ggacttgcgc cacgggaaca acggcttcgg agtacgcgcg gacatgg
//
LOCUS       DAOQJH010000649         1737 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_60695_length_1737_cov_3.810939, whole genome shotgun sequence.
ACCESSION   DAOQJH010000649 DAOQJH010000000
VERSION     DAOQJH010000649.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1737
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60695_length_1737_cov_3.810939"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..467
                     /locus_tag="PLQ18_12815"
     CDS             <1..467
                     /locus_tag="PLQ18_12815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015280601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HNE02581.1"
                     /translation="TRTAAGDQSGALLVERLQDAGHQLVARALEPDDLYRLRARVSQW
                     IADAAVEVVLVTGGTGPTGRDRTPEALRPLLDKELEGFGELFRQLSYQEIGTAAAFSR
                     ALAGVANGTLVVALPGSPGACRTAWDGILAGQLDSRRGHCNYVALMPRLTET"
     gene            complement(498..833)
                     /locus_tag="PLQ18_12820"
     CDS             complement(498..833)
                     /locus_tag="PLQ18_12820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02582.1"
                     /translation="MSASVALPVADHRLAVLLRRYEDILANSCPAFSDAEWSALSEAL
                     ADINLEETDALRRLYATVCHEIQRRGLDGKWHIDARALMHRLGGLSRAEEAALVEIVE
                     FRLGRGRGI"
     gene            complement(1029..>1737)
                     /locus_tag="PLQ18_12825"
     CDS             complement(1029..>1737)
                     /locus_tag="PLQ18_12825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02583.1"
                     /translation="SITAPYPRWLRTGLAPVSVAALGLASLDAGWPLPDWLCHAAALA
                     AGAALLGFAACTLWLQHRRRRRLPDPWLGYWKLALGCAALAVCLWLAGRFGLRTGSGG
                     SGSSTEMLSGLLWLLGFGTTLVAGMLQRIVAFLVWLDLSTRLRAVRSAALAPNVRELI
                     PAAALQRQFAAQFLSVGLTLAACTLPALARPAGAAGVLAWGLLALNLVRAIRTGRGWR
                     QRIGEGSAAVAEQSVTI"
BASE COUNT          262 a          605 c          658 g          212 t
ORIGIN      
        1 atacccgcac ggctgccggg gaccagtccg gcgcgctgct cgtcgagcgg ctgcaggacg
       61 ccggtcacca gctggtggcg cgggcgctgg agccggacga cctgtaccgg ctgcgcgccc
      121 gcgtgtcgca gtggatcgcc gatgcggcgg tcgaggtcgt gctggtcacc ggcggcaccg
      181 ggccgaccgg acgcgatcgc acgccggaag ccctgcgccc gctgctcgac aaggaactcg
      241 aaggtttcgg ggaactgttc cggcagctct cgtaccagga aatcggtacg gccgcggcgt
      301 tctcgcgggc gctggccggc gtggccaatg gcacgctggt cgtggcgctg ccgggttcgc
      361 ccggcgcctg ccgcacggcc tgggacggca tcctcgccgg gcagctcgac agccgccgcg
      421 ggcactgcaa ctacgtggca ctgatgccgc ggctcaccga aacctgagcc cggacggtgg
      481 ccggcggtgc aggcgattca gatgccgcgg ccgcggccga ggcggaactc gacgatctcg
      541 accagcgcgg cctcctcggc acggctcagg ccgccgaggc ggtgcatcag cgcgcgcgcg
      601 tcgatgtgcc acttgccgtc gagaccgcgg cgctggattt cgtggcagac ggtcgcgtac
      661 agacggcgca gcgcatcggt ttcctcgagg ttgatgtcgg cgagggcttc ggacagcgcc
      721 gaccattcgg catcggaaaa ggccgggcag gagttggcga ggatgtcctc gtagcgacgc
      781 agcagcacgg cgagccggtg atcggcgacg ggcagggcaa cggaagcaga catgggggaa
      841 aacctgatcg gtcgggtttg cactggagag ttcgatgacc cgtaccgatc gcttccgtgc
      901 gcgctgcggg ccgttttccg gtgcgccgca cggaggtgcg gaacgccggt tttgcgtgcc
      961 gctagttctg gcatgggtac ccggcagccg caagccgggt cggccggaga aatggtcttg
     1021 tttacgattc agatcgttac agactgttcc gccaccgccg cgctgccctc cccgatccgc
     1081 tgccgccagc cgcgtcccgt ccggatcgcc cgcacgaggt tcagggccag cagcccccag
     1141 gcgagcacgc cggcggcacc ggccggccgg gccagggccg gcagcgtgca ggccgccagc
     1201 gtcagcccca ccgacaggaa ctgggccgcg aactggcgct gcagggcggc ggccgggatc
     1261 agctcgcgga cgttcggcgc cagggcggcg ctgcggaccg cgcgcaggcg ggtcgagaga
     1321 tccagccaga ccagaaacgc gacgatgcgc tgcagcatgc cggcgaccag ggtcgtgccg
     1381 aagccgagga gccacagcag gccgctcagc atttcggtgg aggaaccgct cccgccgctg
     1441 ccggtgcgca gcccgaaccg cccggcgagc cagaggcaga ccgcgagggc ggcgcagccg
     1501 agcgcgagct tccagtagcc gagccacgga tccggcagcc ggcgccggcg ccggtgctgc
     1561 agccacagcg tgcaggcggc gaagccgagc agcgccgcgc cggcggccag cgccgcggca
     1621 tggcacagcc agtccggcag cggccagccg gcatcgaggc tcgccagccc cagcgcggcg
     1681 acgctcaccg gggcgagccc ggtgcgcagc cagcgcggat acggggccgt gatcgag
//
LOCUS       DAOQJH010000650         1735 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_60831_length_1735_cov_3.093452, whole genome shotgun sequence.
ACCESSION   DAOQJH010000650 DAOQJH010000000
VERSION     DAOQJH010000650.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1735)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1735)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1735
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60831_length_1735_cov_3.093452"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(76..807)
                     /locus_tag="PLQ18_12830"
     CDS             complement(76..807)
                     /locus_tag="PLQ18_12830"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HNE02584.1"
                     /translation="MRHCPALLRPAALLLGLALASPAPAAPLPALTARYALIAHGLTV
                     ARAELLSRPTPAGYELHARVEGGGLTGLFGELGRAELTGAAVPGRPDSRPLSFLVHSK
                     PPFTSATTLLYDWPAGRLHARHGESASDLPLEADLADPLAFFVELLEARRSGRPAPPR
                     LRVPYASGIVEYHLDSLGRETVPTALGARDAEKYELSRPDGGSGRVRITLWLSPADDW
                     LPVLAVRSRKHKERARAEIESLSRP"
     gene            complement(868..1290)
                     /locus_tag="PLQ18_12835"
     CDS             complement(868..1290)
                     /locus_tag="PLQ18_12835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014702677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HNE02585.1"
                     /translation="MIHQEILIVRTPGRCTTDLSTQIGAVVGRSQVRTGIAHVFVQHT
                     SASLMLCENADPSVRRDLETFMARLAPDGAPWYAHDMEGPDDMPAHIRSVLTSNGLTL
                     PVGEGRLLLGTWQGVYLWEHRQSSHQRRLVVTVQGEQH"
     gene            complement(1287..>1735)
                     /locus_tag="PLQ18_12840"
     CDS             complement(1287..>1735)
                     /locus_tag="PLQ18_12840"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HNE02586.1"
                     /translation="YRHERRGGRERESVRIDFDPAAGEVRIEEAGSAPAKLALAPGML
                     DPLSLQLRAMWLLRQGRPPESFTLVDDASPDTYRVESAGRETVKTGRGPVEAEVLRRG
                     RPGDKRTATFWFAPKFGYVPVQIVQTRKGRETLRMTLIDVSGEAVR"
BASE COUNT          227 a          602 c          655 g          251 t
ORIGIN      
        1 gattgccgat ccgctgatgc ccttccagag catccagtcc ggcgagcagc gatcggcaga
       61 ggcggcaggg cgtcttcatg gccggctcag gctctcgatc tcggcgcgcg cgcgttcctt
      121 gtgcttgcgg ctgcgcacgg cgagcaccgg cagccagtca tcggccggtg acagccacag
      181 cgtgatgcgc acgcgcccag agccgccgtc cggacgcgac agctcgtact tctcggcgtc
      241 gcgcgcaccg agtgcggtcg gcacggtttc gcggccgagg ctgtcgaggt ggtactcgac
      301 gatgccggag gcatacggca cgcgcagccg cggcggcgcc gggcggccgc tgcgccgggc
      361 ttcgagcagc tctacgaaga aggcgagcgg atcggcgaga tcggcttcga gcggcaggtc
      421 gctggcgctc tcgccgtggc gggcatgcag cctgccggcc ggccagtcgt agagcagcgt
      481 cgtagccgac gtgaacggcg gcttcgaatg cacgaggaag ctgagcggcc ggctgtccgg
      541 gcggccgggc accgcggcgc cggtcagctc ggcgcggccg agttcgccga acagcccggt
      601 caggccgccg ccctcgacgc gggcgtgcag ctcgtagccg gccggtgtcg gccggctcag
      661 cagctcggcg cgcgcgacgg tcaggccgtg cgcgatcagc gcgtagcggg cggtcagcgc
      721 cggcagcggt gcggccggcg ccgggctggc gagggcgagg ccgagcagca gtgcggccgg
      781 acggagcagg gcagggcagt ggcgcatggc ggtcagacgc gacgcagcgt ttccgggttc
      841 gcgcgcgcag tgcgtttcgt gcgccgctca gtgctgttcg ccctgcacgg tgaccaccag
      901 ccggcgctgg tggctggact ggcggtgttc ccacagatac acgccctgcc aggtgccgag
      961 cagcagccgg ccctcgccga ccggcagcgt caggccattc gaggtcagca cgctgcggat
     1021 gtgtgccggc atgtcgtccg ggccttccat gtcgtgggcg taccacggcg cgccatcggg
     1081 cgcgagccgg gccatgaagg tttcgagatc gcggcgcacc gacgggtcgg cattctcgca
     1141 gagcatcagc gaggcgctgg tgtgctgcac gaacacgtgg gcgattccgg tgcgtacctg
     1201 actgcgcccg accacggcgc cgatctgcgt gctgaggtcg gtggtgcagc gccccggcgt
     1261 gcgcacgatc aggatttcct ggtgaatcat cggacggctt ccccgctcac gtcgatcaag
     1321 gtcatgcgca gggtttcgcg ccccttgcgc gtctgcacga tctgcaccgg cacgtaaccg
     1381 aacttcggcg cgaaccagaa ggtggcggtg cgcttgtcgc cgggccggcc gcggcgcagc
     1441 acctcggcct cgaccgggcc gcggccggtc ttcacggtct cgcgcccggc gctctcgacc
     1501 cggtaggtat cggggctggc gtcatcgacc agcgtgaagc tctccggcgg ccggccctgt
     1561 cgcagcagcc acatcgcgcg cagctgcagg ctcagcggat cgagcatgcc gggggcgagc
     1621 gcgagcttcg ccggcgccga gccggcctcc tcgatccgca cctcgccggc cgccggatcg
     1681 aagtcgatgc gcaccgattc gcgctcgcgt ccgccgcggc gctcgtggcg gtagt
//
LOCUS       DAOQJH010000651         1730 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_61175_length_1730_cov_3.530746, whole genome shotgun sequence.
ACCESSION   DAOQJH010000651 DAOQJH010000000
VERSION     DAOQJH010000651.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1730)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1730)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1730
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61175_length_1730_cov_3.530746"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            161..1531
                     /gene="purB"
                     /locus_tag="PLQ18_12845"
     CDS             161..1531
                     /gene="purB"
                     /locus_tag="PLQ18_12845"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009683124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HNE02587.1"
                     /translation="MDLTALTAVTPVDGRYADKTADLRPIFSEYGLIRHRVLVEVRWL
                     QRLAKHPGIPEVPELSEHAGHVLNHLVEHFTVEDAARVKNIERTTNHDVKAVEYFIKE
                     KIAGNAELAAVSEFVHFACTSEDINNLSYALMLKAARTQAVLPQMDAIIDAIRRLAHL
                     HADVPMLSRTHGQPASPSTLGKEMANVVYRLKRTREQVAAVPLLGKINGAVGNYNAHL
                     SAYPEIDWEAHARSFVEDDLGLTLNPYTIQIEPHDYMAELFDATARFNTILIDFSRDV
                     WGYIALGFFKQRTIAGEVGSSTMPHKVNPIDFENAEGNFGIANALLTHFAQKLPVSRW
                     QRDLTDSTVLRAIGTAFAHAQIGYASLLKGIGKLEVNAAALAADLDANWEVLAEPIQT
                     VMRRYGIEQPYEKLKALTRGQRLDAAAMRAFIETLELPAEARARLLELSPATYVGNAA
                     EQARRV"
BASE COUNT          297 a          623 c          560 g          250 t
ORIGIN      
        1 gggagacctc gacggtgtcg gcggcaagcc gcgggtgcag cgtgaacatg ggcgtacctt
       61 cgggtcggag cgggtgcggt gtatcatccg cgcccttcgc tgcgggccgt caagcccgtg
      121 cttccccccg ctcgcccgcg ccgactcccg gagcccgccg atggacctca ccgccctcac
      181 cgccgtcact ccggttgacg gccgttacgc cgacaagacc gcggacctgc ggccgatctt
      241 cagcgagtac gggctgatcc gccatcgcgt gctggtcgag gtgcgctggc tgcagcggct
      301 ggcgaagcat ccgggcatcc ccgaggtgcc ggagctctcc gagcacgccg ggcatgtgct
      361 gaaccatctg gtcgagcact tcacggtcga ggatgccgcg cgcgtcaaga acatcgagcg
      421 cacgaccaac cacgacgtga aggccgtcga gtacttcatc aaggagaaga tcgccggcaa
      481 cgccgagctg gcggcggtca gcgagttcgt gcacttcgcg tgcacctccg aggacatcaa
      541 caacctttcc tatgcgctga tgctgaaggc agcgcgcacg caggccgtgc tgccgcagat
      601 ggacgcgatc atcgacgcga tccgccggct cgcccacctg catgccgacg tgccgatgct
      661 gtcgcgcacg cacggccagc cggcctcgcc ctccacgctc ggcaaggaga tggccaacgt
      721 cgtgtaccgg ctcaagcgca cgcgcgagca ggtcgcggcc gtgccgctgc tcggcaagat
      781 caacggtgcg gtcggcaact acaacgcgca cctgtcggcg tatccggaaa tcgactggga
      841 ggcgcacgcg cgcagcttcg tcgaggacga cctgggcctg acgctgaacc cgtacacgat
      901 ccagatcgag ccgcacgact acatggccga gctgttcgat gcgacggcgc gcttcaacac
      961 catcctgatc gacttctcgc gcgacgtgtg gggctacatc gcgctcggct tcttcaagca
     1021 gcgcacgatc gccggcgagg tcggttcgtc gacgatgccg cacaaggtca atccgatcga
     1081 cttcgagaac gccgagggca acttcggcat cgccaatgcg ctgctcacgc acttcgcgca
     1141 gaagctgccg gtgtcgcgct ggcagcgtga cctgaccgac tcgaccgtgc tgcgcgcgat
     1201 cggcacggcc ttcgcgcacg cgcagatcgg ctatgcgtcg ctgctcaagg gcatcggcaa
     1261 gctcgaagtc aacgccgccg cactcgcggc cgatctcgat gccaactggg aagtgctggc
     1321 cgagccgatc cagaccgtca tgcgccgcta cggcatcgaa cagccctacg agaagctcaa
     1381 ggccctgacc cgcggccagc gcctcgatgc cgcggcgatg cgcgccttca tcgagacgct
     1441 ggaactgccg gccgaggcca gggcgcggct gctggagctg agcccggcca cgtatgtcgg
     1501 caacgcggcc gagcaggcgc ggcgggtctg aacgaaactc tcacctgtaa gcccggacga
     1561 atccgccatc ggcagggaag tccgtggccg gcaaggagcc ggcccgcacc ccgcccggca
     1621 tccgcgcctg cgcagcccgc agcggcgaat ccctgagcag cgaggcgcgg cctgagcctt
     1681 cgtccgcgcc cctttcccga ttccgccccc caccgatgga caccgcgcca
//
LOCUS       DAOQJH010000652         1729 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_61244_length_1729_cov_3.738351, whole genome shotgun sequence.
ACCESSION   DAOQJH010000652 DAOQJH010000000
VERSION     DAOQJH010000652.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1729)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1729)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1729
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61244_length_1729_cov_3.738351"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="PLQ18_12850"
     CDS             <1..226
                     /locus_tag="PLQ18_12850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018865864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1244 domain-containing protein"
                     /protein_id="HNE02588.1"
                     /translation="VQNIDLMNLAGFCRNCLSKWYLAAAQARGLALDYEGAREIVYGM
                     PYEQWKTLHQREASPEQQTAFAEAIRKVHD"
     gene            complement(265..699)
                     /locus_tag="PLQ18_12855"
     CDS             complement(265..699)
                     /locus_tag="PLQ18_12855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02589.1"
                     /translation="MPVAAYHRWTYNRQDGRSSATLDEPSDTTGSTPVRNVVEFFNPR
                     PIIGVFVDGLAGCDWQCPGTEDAESAELQDVNLLPSGHLVLTLLLRGADEPTEWIGLN
                     GVLDATDGSMALELGGTRYLAFPPRERERVSALFLKLANRQY"
     gene            729..>1729
                     /locus_tag="PLQ18_12860"
     CDS             729..>1729
                     /locus_tag="PLQ18_12860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02590.1"
                     /translation="MSMSKPVRPGGGRRVGLMLVIVVLATALWYGWQVPELRHRLIYG
                     FEHWVAGLQTRFNIRPPGAEPEPTAGEVELLKPPAALRPGTAPAAAAQAPAAQGIRDP
                     SITDDEALLSGAQPMPTLAAPRSLKFAVGEEAVFGLELLPGIAARGQRVIIGGLPMDA
                     SLSAGARDEMGRWVLDAVALPGLKLRSGAPGHYTLLIRTQPLQRLRVPRPVAEAAVEV
                     TVLPAAGAEARTAAPPAAKPPQPGPGELLDRGRRALELGDLGAARLYLEAAAGQNSAD
                     AALLLAASYDPGRSGDNADVGLAADWYLRARKLGAAEAQPAIDALRSWLQARTEPGEA
                     "
BASE COUNT          258 a          592 c          626 g          253 t
ORIGIN      
        1 cgtgcagaac atcgacctga tgaacctcgc cggcttctgc cgcaactgcc tgtcgaagtg
       61 gtatctggcc gcggcgcagg cacgcggact cgctctcgac tacgaaggcg cccgcgagat
      121 cgtctacggc atgccttacg agcagtggaa aaccctgcac cagcgcgaag ccagccccga
      181 acagcagacc gccttcgccg aggcgatccg caaggtgcat gactgaaggt gcatgactga
      241 aagagcgggg cggcccggtg cgcgtcagta ctggcggttc gcgagtttca ggaacagcgc
      301 gctgacgcgt tcgcgttccc ggggcggaaa cgccagatag cgcgtgccgc ccagttccag
      361 cgccatcgag ccgtcggtcg catcgagcac gccgttcaga ccgatccatt cggtcggctc
      421 gtcggcgccg cgcagcagca gcgtcagcac cagatggccg gacggcagca ggttcacgtc
      481 ctgcagttcg gctgattcgg catcctcggt gcccgggcat tgccagtcac agcctgccag
      541 tccatcgacg aacacgccga tgatcggccg tggattgaag aattcgacta cgttgcgcac
      601 gggtgtcgag cctgtggtgt cggacggctc gtcgagcgtt gcgctggaac ggccgtcctg
      661 tcggttgtag gtccatcggt ggtacgcggc aacgggcatg ccgcgctccg gaaaaggtgc
      721 cagtgatcat gagtatgagc aagccggtca ggccgggtgg cggcaggcgg gtgggcctga
      781 tgctcgtcat cgtcgtgctg gcgacagcgc tctggtatgg atggcaggtg ccggagctgc
      841 gccaccggct catctacggc ttcgagcact gggtcgcggg cctgcagacg cgtttcaaca
      901 tccgtccgcc gggtgccgag cccgagccca cggccggcga ggtcgagttg ctgaaacccc
      961 cggcggcact gcgcccgggc actgccccgg ccgcggcggc gcaggccccg gccgcacaag
     1021 gtatccgcga tccgtccatc accgacgacg aggcgctgct gtcgggcgcg cagccgatgc
     1081 cgacgctggc ggctccgcgc tcgctgaagt tcgccgtcgg cgaggaggcg gtgttcgggc
     1141 tggaactgct gcccggcatc gctgcgcgcg ggcagcgcgt catcatcggc ggactgccga
     1201 tggatgccag cctgagcgcc ggtgcccgcg acgagatggg gcgctgggtg ctggatgccg
     1261 tcgccctgcc ggggctgaag ctgcgcagcg gcgcaccggg tcattacacc ctgctgatcc
     1321 gcacgcagcc gctgcagcgc ctgcgcgtgc cccggccggt cgccgaggcc gcggtcgagg
     1381 tcacggtgct gccggcggca ggggccgagg cccggaccgc ggcgcccccg gccgcgaaac
     1441 cgccgcagcc cggtcccggc gaactgctcg atcgcggacg ccgcgcgctg gaacttggtg
     1501 acctgggcgc ggcgcgtctg tatctggaag ccgcggccgg gcagaacagc gccgatgccg
     1561 cgctgctgct cgcggccagc tacgatccgg ggcgcagcgg cgacaacgcc gatgtcgggc
     1621 tcgccgcgga ctggtatctg cgggcacgca agctcggtgc tgccgaggcc cagcccgcca
     1681 tcgatgcgct gcgcagctgg ctgcaggccc gcaccgagcc cggcgaggc
//
LOCUS       DAOQJH010000653         1729 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_61245_length_1729_cov_3.627838, whole genome shotgun sequence.
ACCESSION   DAOQJH010000653 DAOQJH010000000
VERSION     DAOQJH010000653.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1729)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1729)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1729
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61245_length_1729_cov_3.627838"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            48..>1729
                     /locus_tag="PLQ18_12865"
     CDS             48..>1729
                     /locus_tag="PLQ18_12865"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation:proton antiporter-2 (CPA2)
                     family protein"
                     /protein_id="HNE02591.1"
                     /translation="MSDLAEFVVILTAIVLAVPLARRCRLGAVIGYLIAGVVIGPSGL
                     GLIRDHEAILHIGELGVVLLLFLIGLELKPSRLWALRRPVFGLGGVQVLATGAVATLA
                     LRLLDYPPAGALVAGLALSLSSTAFVLQLLAEQGELGSRHGRDAFAVLLLQDVAVIPM
                     LALLPLLATEARPGDEGDGLRQAAVAVAAITVAVLASRYLVPRLFRLIAASRSHEVAT
                     AGALLVVAAAALGCAEIGLSMSLGAFLIGLLLADSEFRHELEANIEPFKGVLLGLFFI
                     AVGMSANLGLLAEAPLRLLGLTLGLMALKAGLMFGVACAWGHAAASAGRVAVLLAQGG
                     EFAFVLFANAAGLGLLDAARVEELSVVVTLSMALTPLLLAALQAGEHWLAARRPRPPG
                     PPLPPISPAPVLIAGYGRFGQTLARFLRVRHVPYTLFDTDVARIELGRQLGETVHYGD
                     ASRVELLRAAHAAHAAVLVIGIDDVETSLRAAATAHRHFPQLRVLSRACNARHTLRLM
                     DLGVQAVASDTYLSALEMAECVGVALGDAPADIRAEVARFREHDQRNLRRLHG"
BASE COUNT          164 a          638 c          638 g          289 t
ORIGIN      
        1 gatcgtgcga cactgggcgg ccgcatcctg tctgtcccgc tgccctcatg tccgatctgg
       61 ccgaattcgt cgtcatcctc accgccatcg tgctggccgt gccgctcgca cgccgctgcc
      121 ggctcggggc cgtgatcggc tatctgatcg ccggcgtcgt catcgggccg tccggactcg
      181 gcctgatccg cgatcacgag gcgatcctgc acatcggcga gctcggcgtc gtgctgctgc
      241 tgttcctgat cggcctggag ctgaagccct cgcggctgtg ggcgctgcgc cggccggtgt
      301 tcgggctcgg cggcgtgcag gtgctcgcga ccggcgccgt cgcgacgctg gcgctgcggc
      361 tgctcgacta cccgccggcc ggtgcgctgg tcgcggggct ggcgctgtcg ctgtcctcga
      421 cggccttcgt gctgcagctg ctcgccgagc agggcgaact cggcagccgc cacggccgcg
      481 atgccttcgc ggtgctgctg ctgcaggacg tggcggtgat cccgatgctg gcgctgctgc
      541 cgctgctcgc caccgaggcc cggcccggcg acgagggcga cgggctgcgg caggcggcgg
      601 tcgcggtcgc ggcgatcacg gtcgcggtgc tcgcgagccg ttacctggtg ccgcggctgt
      661 tccggctgat cgccgccagc cgctcgcacg aggtcgccac cgccggcgcg ctgctggtgg
      721 tggccgccgc cgcgctcggc tgcgccgaga tcggcctcag catgtcgctc ggcgccttcc
      781 tgatcggcct gctgctcgcc gattccgagt tccgccacga gctcgaagcc aacatcgagc
      841 cgttcaaggg cgtgctgctc gggctgttct tcatcgcggt cggcatgtcg gccaacctcg
      901 gcctgctcgc cgaggcgccg ctgcgcctgc tcggcctgac cctcgggctg atggccctga
      961 aggcggggct gatgttcggg gtcgcgtgcg cctggggtca tgccgcggcc agcgccggcc
     1021 gggtcgcggt actgctcgcg cagggcggcg agttcgcgtt cgtgctgttc gccaatgccg
     1081 ccggtctcgg cctgctcgat gcggcgcgcg tcgaagagct gagcgtggtc gtgacgctgt
     1141 cgatggcgct gacgccgctg ctgctcgccg ccctgcaggc cggcgagcac tggctggccg
     1201 cgcgccggcc gcggccgccg ggtccgccgc tgccgccgat ctcgccggcg ccggtgctga
     1261 tcgccggcta cggccgcttc ggccagacgc tggcgcgctt cctgcgcgtg cgccacgtgc
     1321 cgtacacgct gttcgacacc gatgtcgcgc gcatcgagct cggccgccag ctcggcgaga
     1381 ccgtgcacta cggcgacgcc tcgcgcgtcg agctgctgcg cgccgcgcac gccgcgcacg
     1441 ccgcggtgct ggtgatcggc atcgacgatg tcgagacctc gctgcgcgcg gcggcgaccg
     1501 cgcaccggca cttcccgcag ctgcgcgtgc tgtcgcgcgc ctgcaacgcc cggcacacgc
     1561 tgcggctgat ggacctcggt gtgcaggcgg tggcgagcga cacctacctg tcggcgctgg
     1621 agatggccga gtgtgtcggc gtcgcgctcg gcgatgcgcc ggctgatatc cgtgccgagg
     1681 tcgcgcgctt ccgcgagcac gaccagcgca acctgcgccg gctgcatgg
//
LOCUS       DAOQJH010000654         1728 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_61319_length_1728_cov_3.108189, whole genome shotgun sequence.
ACCESSION   DAOQJH010000654 DAOQJH010000000
VERSION     DAOQJH010000654.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1728)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1728)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1728
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61319_length_1728_cov_3.108189"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="PLQ18_12870"
     CDS             <1..237
                     /locus_tag="PLQ18_12870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PE family protein"
                     /protein_id="HNE02592.1"
                     /translation="GGNGADGQSQPTDFSLGFYGGSAGGSGGSSLVGNGGNGGKGGWP
                     GGGGSGGGAAVDSVGNSGAGGDGADGAAEIITLF"
     gene            249..464
                     /locus_tag="PLQ18_12875"
     CDS             249..464
                     /locus_tag="PLQ18_12875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02593.1"
                     /translation="MSTGRFAVVIVSISTVERVTVGRPDVPATKIAVELMLGEAAGPD
                     FKYNPDGNPRFTAPPDEPPPPDEPPAE"
     gene            516..827
                     /locus_tag="PLQ18_12880"
     CDS             516..827
                     /locus_tag="PLQ18_12880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02594.1"
                     /translation="MTFAALTARANLALYRVFGEAATCGGLACVVILSRASNTGPTGY
                     DAQVSGQSVTAVVRVGEGDEMLPAAPIAGALLVVGDESWRVMEPPARTGEAGEYRLVL
                     A"
     gene            824..1495
                     /locus_tag="PLQ18_12885"
     CDS             824..1495
                     /locus_tag="PLQ18_12885"
                     /inference="COORDINATES: protein motif:HMM:NF018472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HNE02595.1"
                     /translation="MTTAVIQIDQAALAEAAAMLTDVRNGTDRALAMAINRVAPRIRT
                     AMSTAVRQQVALRAAYVNAALELQRARIGRMRASISTPERGVPLVQYMTSSRLMTFKV
                     GKPATKRLIQAPFVRVAAGGKLTRVQGESLGGGKFSGAWVGTTRRGITQIFTPVDPGD
                     RRWGRPIRPLYGPSISQVLSQSKTGKTLIDEAAQSASDRLPVELLQAATYLLTKQAAR
                     VNPDG"
     gene            1488..>1728
                     /locus_tag="PLQ18_12890"
     CDS             1488..>1728
                     /locus_tag="PLQ18_12890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02596.1"
                     /translation="MVDSVRERIVSVLLTRTSAARAYTRPDALPARGLFVGPDTDGQT
                     PVYGGQQLTLTATLEQVESVPTPGADIAAFVAAVDA"
BASE COUNT          254 a          605 c          620 g          249 t
ORIGIN      
        1 ggcggaaatg gcgcagacgg gcagagccag ccgactgact tttccctcgg cttctacggc
       61 ggctctgccg gcgggtcagg cggtagcagc ctggtcggca atggcggcaa tggcggcaaa
      121 ggcggctggc cgggcggagg cgggtcgggc ggcggcgcgg ctgtcgattc tgttggcaat
      181 tccggggccg gcggcgatgg cgccgacggg gccgcggaaa tcatcacgtt gttctgacga
      241 ggtatcgaat gagcacaggt cgttttgcgg tagtaatcgt ctcgatcagc acggtcgagc
      301 gcgtcacggt cgggcgtccg gatgtgccgg cgacgaagat cgccgtcgag ctgatgctcg
      361 gcgaggccgc aggcccggac ttcaagtaca acccggacgg caatccgcgc ttcacggccc
      421 cgccggatga gccgccgccg ccggatgagc cgccggcgga gtgatgcgcg cgtgaacctc
      481 gccttcggcc gcggcctcga cgggtgcgcc acgccatgac cttcgccgcc ctcactgccc
      541 gcgccaacct cgcgctctac cgcgtgttcg gcgaggccgc gacctgcggc ggcctcgcct
      601 gcgtcgtcat cctgtcccgc gcgagcaaca ccggcccaac cggctacgac gcgcaggtat
      661 ccgggcagtc ggtcaccgcc gtcgtgcggg tcggggaggg agacgagatg ctgccggccg
      721 caccgatcgc cggcgcgctg ctcgtggtcg gcgacgagtc gtggcgcgtg atggagccgc
      781 cggcgcgcac cggcgaggcc ggcgagtacc ggctggtgct ggcgtgacga ccgccgtcat
      841 ccagatcgac caggccgcgc tcgccgaagc cgccgcgatg ctcacggacg tgcgtaacgg
      901 cactgaccgc gcgctggcga tggcgatcaa ccgcgttgcg ccgcgcatcc gcacagccat
      961 gtcaaccgcc gtgcgccagc aggtcgcgct gcgtgcggcc tacgtcaatg ccgcgctgga
     1021 actgcagcgc gcccgcatcg gacggatgcg cgccagcatc agcacgccgg agcgcggcgt
     1081 gccgctggtg cagtacatga cgagttcgcg gctgatgacc ttcaaggtcg gaaagcctgc
     1141 gaccaagcgc ctgatccagg cgccgttcgt gcgcgtggcg gccggcggca agctcacgcg
     1201 cgtgcagggc gaatcgctcg ggggcggcaa gttctccggc gcgtgggtcg gcacgacccg
     1261 ccgcggcatc acgcagattt tcaccccggt cgatccggga gatcgccggt ggggccggcc
     1321 aatcaggccg ctgtacggtc cgtcaatttc gcaagtcctg tcgcagagca agaccggcaa
     1381 gaccctgatc gacgaagccg cgcagtccgc gtccgaccgg ctgccggtag agctgctgca
     1441 ggccgccacc tacctgctga cgaagcaggc tgcccgagtg aatccagatg gttgactccg
     1501 ttcgcgagcg catcgtcagc gtgctgctca cgcgcacctc tgccgcccgc gcctacacgc
     1561 gcccggacgc gctgccggcc cgcggcctgt tcgttggccc ggacaccgac gggcagaccc
     1621 cggtctacgg cggtcagcag ttgacgctga cggccacgct ggagcaggtc gagtcagtgc
     1681 cgacgcccgg cgccgacatc gccgcgttcg tcgctgcggt cgatgccc
//
LOCUS       DAOQJH010000655         1728 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_61324_length_1728_cov_2.940227, whole genome shotgun sequence.
ACCESSION   DAOQJH010000655 DAOQJH010000000
VERSION     DAOQJH010000655.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1728)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1728)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1728
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61324_length_1728_cov_2.940227"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..440)
                     /locus_tag="PLQ18_12895"
     CDS             complement(<1..440)
                     /locus_tag="PLQ18_12895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HNE02597.1"
                     /translation="MFPMRPALLPALALLCAMPVVPWVESHAETRAEARADSLRAALD
                     AAWARTAAAQAHEARRGSVAAQARLADDWLAGPPVLDAELRSDRATGNHGARELDLGV
                     ELPLWQAGEAEARARLAGHDAERLGAAQLAQRLPLAGQVREAAWT"
     gene            complement(548..1009)
                     /locus_tag="PLQ18_12900"
     CDS             complement(548..1009)
                     /locus_tag="PLQ18_12900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02598.1"
                     /translation="MLRRLRSACLRACLSLLLMLTLPLQALAAVAPVWLPHGHAGLMA
                     ADGSVPAHRHALHAHAHGLGVHALTDGASASASASELHGPDCAGHRHAAHEAAAGADD
                     GDCGAHWFVSAAMPTVAGALRLPPSRPVRHAGLVALHPLRLVDPPARPPRA"
     gene            1233..>1728
                     /locus_tag="PLQ18_12905"
     CDS             1233..>1728
                     /locus_tag="PLQ18_12905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014194473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNE02599.1"
                     /translation="MAYDIIGDIHGQADKLLALLQRLGYRQRAGAWRHPQRQVIFVGD
                     YIDRGPAQLRTLDLVRRMTEAGSALAILGNHELNAIAWHTPDPRAPGEYLRPHFRPGR
                     GEKNRRQHAAFLAEVEHRPALHAELIDWFLSLPLWLELPGLRVVHACWHAPFLEWLRP
                     QLRDG"
BASE COUNT          251 a          633 c          651 g          193 t
ORIGIN      
        1 gtccaggccg cctcgcgcac ctgcccggcc agcggcaggc gctgcgcgag ctgcgcggcg
       61 ccgagccgct cggcatcgtg tccggccagg cgcgcacgcg cctcggcctc cccggcctgc
      121 cacagcggca gctcgacgcc gaggtcgagc tcgcgggcac cgtgattgcc ggtggcgcgg
      181 tcgctgcgca gctcggcatc gagcaccggc ggcccggcca gccagtcatc ggccaggcgg
      241 gcctgcgcgg ccaccgagcc gcggcgcgcc tcgtgtgcct gcgcggcggc ggtacgcgcc
      301 cacgcggcat cgagcgcggc gcgcaggctg tcggcacggg cttccgcgcg ggtttccgcg
      361 tgggattcga cccagggaac aacaggcatg gcgcacagca gcgccagcgc cggcagcagc
      421 gccggtcgca tcgggaacat cggcatggct ctccccccgc gggagtgacg gcatcggcag
      481 cgcgcgcctg cgaacggacg cgcgcacggc gaaacccgcg ccggcgttcg ggccgggcgg
      541 tgcggggtca ggcgcggggc gggcgggcgg ggggatcgac gagtcgcagc ggatgcaggg
      601 cgacgagacc ggcgtggcgc accggccggc tcggcggcag cctcagcgcg cccgcgaccg
      661 tcggcatcgc ggcactgacg aaccagtgcg cgccgcaatc gccgtcatcg gccccggcag
      721 cggcctcgtg ggcggcgtgc cgatggccgg cacagtcagg gccgtgaagc tccgatgccg
      781 atgctgatgc tgatgctccg tcggtcagcg catgcacgcc gagcccgtgc gcatgcgcgt
      841 gcagcgcatg ccgatgcgcc ggcaccgaac catcggccgc catcagcccc gcatgcccgt
      901 gcggcagcca gaccggcgcc acggcggcga gcgcctgcag cggcagggtc agcatcagca
      961 gcagggacag gcaggctcgc aggcaggcgg agcgcagacg gcgcagcatc ggcggatcgg
     1021 atcgggtggc cggaggaaag gggcgtaacg gtaacggctg gcgacggcag gcgtccagcc
     1081 atgacacgcg gcagggcgcc ggcgtggcag aagaccgaag gccgaaggct tgtcctgccg
     1141 gtcacgacgg aatacaccgg ccgctcagcg ccttccccgc gcagggtcgg gcgacgcaag
     1201 acaatcacgc atccacctgt gacgggcctg ccatggcata cgacatcatc ggcgacatcc
     1261 acgggcaggc cgacaagctg ctggcgctgc tgcagcggct cggctaccgg cagcgggccg
     1321 gggcctggcg acatccgcag cggcaggtga tcttcgtcgg cgactacatc gaccgcgggc
     1381 cggcgcagct gcgcacgctg gacctcgtgc gccggatgac ggaagccggt tcggcgctgg
     1441 cgatcctggg caaccacgaa ctgaacgcca tcgcctggca tacgcccgat ccccgcgcac
     1501 cgggggaata cctgcgcccg catttccggc cgggccgggg cgagaagaac cgccgccagc
     1561 acgcggcctt cctcgccgaa gtcgagcacc ggccggcgct gcacgccgaa ctcatcgact
     1621 ggttcctgag cctgccgctg tggctggagc tgccgggcct gcgcgtcgtg catgcctgct
     1681 ggcatgcgcc cttcctcgaa tggctccgcc cgcagctgcg cgacgggc
//
LOCUS       DAOQJH010000656         1723 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_61632_length_1723_cov_4.025180, whole genome shotgun sequence.
ACCESSION   DAOQJH010000656 DAOQJH010000000
VERSION     DAOQJH010000656.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1723)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1723)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1723
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61632_length_1723_cov_4.025180"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="PLQ18_12910"
     CDS             complement(<1..188)
                     /locus_tag="PLQ18_12910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HNE02600.1"
                     /translation="MSIAAADSWVVLGRVAGLWGVRGGLKIWSDTQPRAAICRYRPLF
                     LGRDGQWREVVPVLGREQG"
     gene            complement(212..472)
                     /gene="rpsP"
                     /locus_tag="PLQ18_12915"
     CDS             complement(212..472)
                     /gene="rpsP"
                     /locus_tag="PLQ18_12915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007231547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HNE02601.1"
                     /translation="MVTIRLARGGAKKRPFYHVVVTDSRNRRDGRYIERLGFFNPIAR
                     GGEVRLNLDAGRVQYWQGNGAQVSERVAALLKEFDKAQAAAA"
     gene            complement(637..>1723)
                     /locus_tag="PLQ18_12920"
     CDS             complement(637..>1723)
                     /locus_tag="PLQ18_12920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007193059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNE02602.1"
                     /translation="LMAGLQGSGKTTSTAKLARFLREREKKKVLVVSCDVYRPAAIQQ
                     LATLAKEVQVEWFASEGGQQPVAIAQAALAYAKRQFFDVLIVDTAGRLHVDAAMMEEI
                     QALHAAVTPVETLFVVDAMTGQDAANTAKAFHEALPLTGVVLTKTDGDARGGAALSIR
                     YLTGKPIKFLGVGEKTAALEPFHPDRVASRILGMGDVLSLVEEVERKVDKEKALKLAE
                     KVKSGKSFDFEDFREQMEQMLAMGGMAGLLDKLPGMDKVPDDLKKQANDKQVRRLIGI
                     VNSMTPQERRFPDLIKGSRKRRIAAGAGVQVQEINRLLKQFEEMRRMMKKMSGGGMMK
                     LMRAMKGKLPMGGLGGLGGPGGLPPFK"
BASE COUNT          264 a          617 c          548 g          294 t
ORIGIN      
        1 ccctgctcgc gaccgagcac cggcacgacc tcgcgccact ggccatcacg accgagaaac
       61 agcggccggt aacggcagat cgcggcgcgc ggttgcgtgt cggaccagat cttcagcccg
      121 ccgcgcacgc cccagagacc ggcgacgcgc ccgagcacga cccagctgtc ggcagcagca
      181 atgctcacgg atgcagcgcc cggcgtgcgg atcaggccgc agccgcctgc gccttgtcga
      241 attccttcag cagcgcggcg acgcgctcgg acacctgcgc gccgttgccc tgccagtact
      301 gcacgcgccc ggcgtcgagg ttcaggcgca cttcgccacc acgggcgatc ggattgaaga
      361 aaccgaggcg ctcgatgtag cgaccgtcgc ggcggttgcg gctgtcggtg acgacgacgt
      421 ggtagaacgg gcgcttcttc gcgccgccgc gcgccagtcg gatggtgacc atagcgatcc
      481 tcttgcgatc cagtttgtac cgggtgtgcc ggtgtgagcc gtgtctggcc cggggacgaa
      541 aagaccgcgc ccgcaggcgc ggtcccggag aaaaccgcgg attgtacgcg aaaacgccga
      601 tggctcaagg gtcggcgcat cgcctcatct gaacgctcac ttgaacggcg gcaggccgcc
      661 gggaccaccg aggcccccga gtccgcccat cggcagcttg cccttcatcg cgcgcatcag
      721 cttcatcatg ccgccgccgg acatcttctt catcatccgg cgcatttcct cgaactgctt
      781 gagcaggcgg ttgatttcct gcacctgcac gccggcgccg gcggcgatgc ggcgcttgcg
      841 cgagcccttg atcaggtccg gaaagcgccg ctcctgcggc gtcatcgagt tgacgatgcc
      901 gatcagccgg cgcacctgct tgtcgttggc ctgcttcttc agatcgtccg gcaccttgtc
      961 catgcccggc agcttgtcga gcagcccggc catgccgccc atcgccagca tctgttccat
     1021 ctgctcgcgg aagtcctcga aatcgaagct cttgccggac ttgaccttct cggcgagctt
     1081 cagcgccttt tccttgtcga ccttgcgctc gacctcctcg accaggctga gcacgtcgcc
     1141 catgccgagg atgcgcgagg cgacgcgatc cggatggaag ggttcgagcg cggcggtctt
     1201 ctcgccgaca ccgaggaact tgatcggctt gccggtcagg tagcggatcg acagcgccgc
     1261 accgccgcgc gcgtcaccgt cggtcttggt cagcacgacg ccggtcagcg gcagcgcctc
     1321 gtggaaggcc ttcgcggtgt tggcggcgtc ctgaccggtc atcgcatcga cgacgaacag
     1381 cgtctcgacc ggcgtcaccg ccgcgtgcag cgcctggatt tcctccatca tcgccgcatc
     1441 gacgtgcagg cggccggcgg tatcgacgat cagcacatcg aagaactgcc gtttcgcgta
     1501 cgcaagggcc gcctgcgcga tcgccaccgg ctgctggccg ccctcgctcg caaaccactc
     1561 gacctggact tccttcgcga gcgtggccag ctgctggatc gccgccggcc ggtagacgtc
     1621 gcagctgacc accagcacct tcttcttttc gcgctcgcgc aggaagcgcg cgagcttggc
     1681 ggtgctggtc gtcttgcccg agccctgcag gccggccatc agg
//
LOCUS       DAOQJH010000657         1722 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_61708_length_1722_cov_4.023395, whole genome shotgun sequence.
ACCESSION   DAOQJH010000657 DAOQJH010000000
VERSION     DAOQJH010000657.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1722)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1722)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1722
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61708_length_1722_cov_4.023395"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="PLQ18_12925"
     CDS             complement(<1..203)
                     /locus_tag="PLQ18_12925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011395679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HNE02603.1"
                     /translation="MTIRHFTTLADCTPAELAYLIRRAGVLKARHRAGERHAPLPGRV
                     LAQIYEKASTRTRVSFEAGIAQLG"
     gene            795..1508
                     /locus_tag="PLQ18_12930"
     CDS             795..1508
                     /locus_tag="PLQ18_12930"
                     /inference="COORDINATES: protein motif:HMM:NF019225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HNE02604.1"
                     /translation="MTLLKTRFLLAPLAALLLGATAPALAAVLSQQLGDIDFPSDPGL
                     PNADPDAINHRIGTVTFTNASGGDPAPFNQFIGSDTTTDFTASWTFSAYGGAILDPVS
                     AATIQIGLYEGDSAATGNQVAAFTVDGVDLTAALNALLEATPAYTGTEVHYTLSLSAA
                     AIAELLDGSATFSLTLQGPGASVIGDTTNNGAGIDFSILSISTDNGGTPPTGVPEPGS
                     LALAASGFGLLAARRRRRG"
     gene            1647..>1722
                     /locus_tag="PLQ18_12935"
     CDS             1647..>1722
                     /locus_tag="PLQ18_12935"
                     /inference="COORDINATES: protein motif:HMM:NF019126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HNE02605.1"
                     /translation="MDPFLGEIRLFGGNFAPQDWAFCNG"
BASE COUNT          278 a          604 c          581 g          259 t
ORIGIN      
        1 ccgagctgcg cgatgccggc ctcgaaggac acgcgcgtgc gcgtcgaggc tttctcgtag
       61 atctgtgcaa gcacgcgccc gggcagcggt gcgtggcgtt cgccggcgcg atgcctagcc
      121 ttgagcacac cggcgcgccg gatcaggtac gcgagttcgg cgggcgtaca gtcggccagc
      181 gtcgtgaagt gtcgaatcgt catctcgggc aatctcctcc ccccagggag cacggcggac
      241 gcgcggagtg cgtcccgcgg tccgtgccgt caccggccgg accacgtacg acgccgtagc
      301 gggccgccgg ggcgctcggc ggtccggtcg gcgaccggtc gtcgggatcg ggtcgccggc
      361 tgccggtcgg cggcggcgcg atccatccac aaagtctgct ccacgatcct gccgggagca
      421 gcctgaaaca ttttgagaca gtttttgaaa ctgtccggcc gtcgcgcccg gatttctggt
      481 ggtttgccgg catcgctgcg gatcgatgca ggctaagtgc gcgtcagcag cggaaatcgg
      541 gcgcggcgga aaaacgggcg agcctaccgg ggacgcccgg gatgggcaag ccgaacctgc
      601 agcaaaagcc ggccggaccc gggataaatc cctgaaccgc cttgcatccg gcaaaagccg
      661 gatactgatg ttgcaagggt gggcctatcc tgtggcggcg gttgtccaga ggccggtaca
      721 caaggaccgg atccatgccg gcggcagccg caccagcggt aacccagccc acacgacaag
      781 gaaatccgat caccatgacc ctcctgaaaa cccgattcct cctcgcgccg ctcgccgcgc
      841 tgctgctggg cgccacggca ccggcgcttg ccgccgtgct gtcgcagcag ctcggtgaca
      901 tcgacttccc gagcgatccc ggtctgccga acgcggatcc cgatgccatc aatcaccgca
      961 tcggcacggt gaccttcacg aatgccagcg gaggcgatcc ggcgccgttc aaccagttca
     1021 tcggctccga cacgacgacg gatttcacgg ccagctggac cttcagcgcc tatggcggcg
     1081 cgatcctcga cccggtgagc gcggcgacga tccagatcgg cctctacgag ggcgattcgg
     1141 ccgcgaccgg caatcaggtg gctgccttca cggtcgatgg cgtcgacctg accgcggcgc
     1201 tgaacgcgct gctcgaagcc acgccggcct acaccggcac cgaagtccac tacacgctgt
     1261 cgctgtcggc ggccgcgatc gccgaactgc tcgatggcag cgcgaccttc agcctgacgc
     1321 tgcagggacc gggcgccagc gtgatcggcg acaccacgaa caacggtgcc ggcatcgatt
     1381 tctcgatcct gagcatcagc accgacaacg ggggcacgcc accgaccggc gtgcccgagc
     1441 ccggcagcct cgcgctggcg gcctcgggct tcggcctgct cgcggcccgg cgccggcggc
     1501 gcggctgagt ccggcgcggc gcggcaagca ctgcttgccg gacggcggac ggacggctgc
     1561 tagtacttga gccctgcagc gtcagtcgca ccggccccgg ggccgggcga ccggcgctgt
     1621 ccttccgggc ctcacggagc cgtctcatgg atccctttct tggagaaatc cgcctgttcg
     1681 gcggtaactt cgccccgcag gactgggcgt tctgcaacgg gc
//
LOCUS       DAOQJH010000658         1720 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_61866_length_1720_cov_4.276877, whole genome shotgun sequence.
ACCESSION   DAOQJH010000658 DAOQJH010000000
VERSION     DAOQJH010000658.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1720)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1720)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1720
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61866_length_1720_cov_4.276877"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1508)
                     /gene="lysS"
                     /locus_tag="PLQ18_12940"
     CDS             complement(<1..1508)
                     /gene="lysS"
                     /locus_tag="PLQ18_12940"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014777805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HNE02606.1"
                     /translation="MTEPAVTPEAAANDETNENKLIAERRQKLAKLREAGNAFPNDFR
                     RDALAGALQGECAALDGPALDAAARRVTVAGRMMAKRIMGKASFTAIKDMSGQIQLFV
                     QRDAVGDDAYTAFKGWDVGDIVGAEGVLMRTKTGELSVRVESIRLLTKSLRPLPEKFH
                     GLTDQEVRYRQRYVDLIMNDDTRRTFAIRNRVIRGIRDYLDARGFMEVETPMMHVIPG
                     GAAARPFVTHHNALDMALYLRIAPELYLKRLVVGGFERVYEINRNFRNEGLSTRHNPE
                     FTMIEFYQAYADYRDLMDLTEDLLRGLALALLGSTDVPYQGEVYDFGKPFARLTIPEA
                     VVAYNEGVTAEDIATLEGARAVAERLKLPVKPGDGIGKVVTTIFEETVEHQLRHPTFI
                     TAYPTEVSPLARRNDDNPEVTDRFEFFIGGREIANGFSELNDAEDQAERFREQVRQKD
                     AGDHEAMHYDADYVRALEFGLPPTAGEGIGIDRLVMLFADQPSIREVILFPH"
     gene            complement(1597..>1720)
                     /gene="prfB"
                     /locus_tag="PLQ18_12945"
     CDS             complement(1597..>1720)
                     /gene="prfB"
                     /locus_tag="PLQ18_12945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012823314.1"
                     /note="recognizes the termination signals UGA and UAA
                     during protein translation a specificity which is
                     dependent on amino acid residues residing in loops of the
                     L-shaped tRNA-like molecule of RF2; in some organisms
                     control of PrfB protein levels is maintained through a +1
                     ribosomal frameshifting mechanism; this protein is similar
                     to release factor 1; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HNE02607.1"
                     /translation="YVLDQSRIKDLRTSHETGNPQAVLDGDLDPFIEASLKSGL"
BASE COUNT          254 a          542 c          610 g          314 t
ORIGIN      
        1 atgtgcggga acagaatgac ctcgcggatc gacggctgat cggcgaacag catcaccagc
       61 cggtcgatgc cgatgccttc gccggcggtc ggcggcaggc cgaattccag ggcccggaca
      121 tagtcggcat cgtagtgcat ggcctcgtga tcgcccgcat ccttctggcg cacctgctcg
      181 cggaaacgct cggcctggtc ctcggcgtcg ttgagttccg aaaaaccgtt ggcgatctcg
      241 cgcccgccga tgaagaactc gaagcggtcg gtcacttcgg ggttgtcgtc gttgcggcgc
      301 gcgagcggcg agacctcggt cgggtacgcg gtgatgaagg tcggatgccg caactggtgc
      361 tcgacggttt cctcgaagat cgtcgtcacc accttgccga tgccgtcacc gggcttgacc
      421 ggcagcttca ggcgctctgc gacggcgcgc gcgccttcga gtgtggcgat gtcttccgcc
      481 gtgacacctt cgttgtaggc gacgaccgcc tccgggatgg tcagccgggc gaacggcttg
      541 ccgaagtcgt agacctcgcc ctgatacggc acgtcggtgc tgccgagcag tgcgagcgcc
      601 agcccgcgca gcaggtcttc ggtcagatcc atcaggtcgc ggtaatcggc gtaggcctga
      661 tagaactcga tcatcgtgaa ctcggggttg tggcgcgtcg acagcccctc gttgcggaaa
      721 ttgcggttga tctcgtagac gcgctcgaag ccgccgacca ccaggcgctt caggtacagc
      781 tccggcgcga tgcgcaggta cagcgccatg tcgagcgcgt tgtgatgcgt gacgaagggc
      841 cgcgccgcgg cgccgcccgg gatcacatgc atcatcggcg tctcgacctc catgaagccg
      901 cgcgcgtcga ggtaatcgcg gatgccgcgg atcacgcggt tgcggatcgc gaaggtgcgc
      961 cgcgtgtcgt cgttcatgat cagatcgacg taacgctggc ggtagcggac ttcctgatcg
     1021 gtcaggccgt ggaacttctc gggcagcggg cgcagcgact tggtcagcag ccggatcgac
     1081 tcgacgcgca ccgacagctc gccggtcttc gtgcgcatca gcacgccctc ggcaccgacg
     1141 atgtcgccga catcccagcc cttgaacgcc gtgtaggcgt catcaccgac cgcgtcgcgc
     1201 tgcacgaaca gctggatctg gccgctcatg tccttgatcg ccgtgaagct cgccttgccc
     1261 atgatgcgct tggccatcat gcgtccggcg accgtgacgc gccgggcggc ggcgtcgagc
     1321 gccgggccgt cgagcgcggc gcattcgccc tgcagcgcgc cggcgagcgc gtcgcggcgg
     1381 aagtcgttcg ggaaggcgtt gccggcttcg cgcagcttcg cgagcttctg gcggcgctcg
     1441 gcgatcagct tgttctcgtt ggtttcgtcg ttggcagcgg cttcgggggt gacggcgggt
     1501 tcggtcatgt cggtcggttc cgggggctga tccgtgcagg cgacggggcg gtgcgacgag
     1561 tcggcgggct gatcgggaaa gggacgggcg ggaggctcag agtccgctct tgaggctcgc
     1621 ctcgatgaac ggatcgaggt cgccatcgag cacggcctgc ggattgccgg tctcgtggct
     1681 ggtgcgcaga tccttgatgc gcgactggtc gagcacgtag
//
LOCUS       DAOQJH010000659         1718 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_62023_length_1718_cov_3.384847, whole genome shotgun sequence.
ACCESSION   DAOQJH010000659 DAOQJH010000000
VERSION     DAOQJH010000659.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1718)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1718)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1718
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62023_length_1718_cov_3.384847"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..489)
                     /locus_tag="PLQ18_12950"
     CDS             complement(<1..489)
                     /locus_tag="PLQ18_12950"
                     /inference="COORDINATES: protein motif:HMM:NF020377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HNE02608.1"
                     /translation="MNQDDFLFSHIELRTERCDSLFMRRLLAGGGYGLHQAIWRLLPC
                     DPEARRDFLYHRIDGAGVKFYVVSARELVDDTGLWRIRSKPYAPQIEAGESPEAALGA
                     FEFVETGPANSNIKAAKFDLHISLLPGVDRTARARLADVRFRVQESIEELLRRAHGAD
                     FED"
     gene            complement(486..1235)
                     /gene="cas5e"
                     /locus_tag="PLQ18_12955"
     CDS             complement(486..1235)
                     /gene="cas5e"
                     /locus_tag="PLQ18_12955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014086016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HNE02609.1"
                     /translation="MDFLVFRLYAPLAAWGGIAVGQERTSDACPGKSAIVGLLGAALG
                     IRRHEEARLVALDAGYGYGVRIDAEGVVLRDYHTAQVPSRVDLKGRPHCTRRDELAPA
                     RATLHTILSFREYRCDALATVALWARSEAPCPLADLAAALRRPRFVLSLGRKSCAPGL
                     PLEPQIIVADHFDTALDAARFAGEQWLGSDDDPLGLQRNDLCLAWDVDAPVSRAAQMV
                     VRRRDEIRSRRRWQFGERSEAQCRLARGSGA"
     gene            complement(1240..>1718)
                     /locus_tag="PLQ18_12960"
     CDS             complement(1240..>1718)
                     /locus_tag="PLQ18_12960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014086015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
                     /protein_id="HNE02610.1"
                     /translation="SGVRIHDAIERHDEDAGAGHVGEAEFGAAVFYLYLCVDRELLCE
                     NLGGDRALTDRALAALVEAATKVAPTGKQNSFASRARASYALLEKGDEQPRQLSVAFL
                     KPVTGSDPLADAIDLLRATRAKMDQVYGPCAAAVAELDALAGTGNLAELQAFAAGD"
BASE COUNT          289 a          582 c          595 g          252 t
ORIGIN      
        1 atcctcaaaa tcggccccat gcgcgcgacg cagcaactcc tcaatcgatt cctgcacgcg
       61 aaaacggacg tcggcgagcc gcgcccgcgc cgtgcggtca acgccgggga gcaggctgat
      121 gtgcaaatcg aacttggccg ccttgatgtt tgagttggcc gggccagtct ctacgaactc
      181 aaacgctccc agcgccgcct cgggcgactc gcccgcctcg atctgcggcg catagggctt
      241 gctgcggatg cgccacaggc cggtgtcatc aacgagttcg cgtgccgaga cgacgtagaa
      301 cttcacgccg gcgccgtcga tgcggtgata caggaagtcg cgccgcgcct ccggatcgca
      361 gggcagcaag cgccagatcg cctgatgcag gccgtaaccc ccgccggcga gcagtctgcg
      421 catgaatagc gaatcgcagc gctcggtccg cagttcgatg tggctgaaca gaaaatcgtc
      481 ctgattcatg ctcctgaccc ccgggcaagc ctgcactggg cttccgatcg ttcaccgaac
      541 tgccagcgac ggcggctgcg gatttcgtcg cgacgccgga cgaccatctg cgcggcgcgg
      601 ctgaccggag catcgacatc ccaggccagg cacaggtcgt tgcgctggag gccgagcgga
      661 tcgtcatcgc tgcccagcca ctgctcaccg gcgaagcggg cggcatcgag cgcggtatcg
      721 aagtgatcgg ccacgatgat ctgcggctcc agcggcaggc ccggggcgca ggacttgcgc
      781 ccgaggctca gcacgaagcg cggccggcgc agcgcagcgg ccagatcggc gagcgggcag
      841 ggcgcttcgc tgcgggccca cagcgcgacc gtggccagtg catcgcagcg gtattcgcga
      901 aacgacagga tggtgtggag cgtggcccga gccggggcga gttcgtcgcg ccgggtgcaa
      961 tgcgggcggc ccttcagatc gacgcgcgag ggcacctgcg cggtgtggta atcgcgcagc
     1021 acgacccctt cggcgtcgat gcgcacgcca tagccgtagc cggcgtcgag cgcgaccagc
     1081 cgcgcttcct cgtggcggcg gatgccgagg gcggccccga gcaggccgac gatcgccgac
     1141 ttgccggggc aggcatcgga cgttcgctcc tgaccgacgg cgatcccgcc ccaggccgca
     1201 agcggcgcgt aaagacgaaa tacgaggaag tccatagcct cagtctccgg ccgcaaacgc
     1261 ctgcagctcg gcaagattgc cggtgccggc gagcgcatcg agttcggcga cggccgccgc
     1321 acacggaccg tagacctgat ccatcttcgc gcgggttgca cgcagcaggt cgatggcatc
     1381 ggcgagcgga tcgctgccgg tgaccggttt caggaaggcg accgacagct ggcgcggctg
     1441 ctcgtcgcct ttctccagca aggcgtagga ggcacgggcg cgggaggcga agctgttctg
     1501 cttgccggtc ggcgcgacct tggtcgcggc ttcgaccagc gcggccaggg cccggtcggt
     1561 gagcgcgcgg tcgccgccca ggttctcgca cagcagttcg cgatccacgc acaggtacag
     1621 ataaaacacc gcggccccga attcggcttc gccgacatgg ccggcgccgg cgtcctcgtc
     1681 gtggcgttct atggcgtcgt gtatccgaac gccggact
//
LOCUS       DAOQJH010000660         1717 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_62102_length_1717_cov_3.105295, whole genome shotgun sequence.
ACCESSION   DAOQJH010000660 DAOQJH010000000
VERSION     DAOQJH010000660.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62102_length_1717_cov_3.105295"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..537)
                     /gene="thiE"
                     /locus_tag="PLQ18_12965"
     CDS             complement(<1..537)
                     /gene="thiE"
                     /locus_tag="PLQ18_12965"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019465030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HNE02611.1"
                     /translation="MTERPRPHGLYAITDPGLIPDGQLLERVSAAIDGGARTIQYRDK
                     RADAARRRQLAAALAGLCRARAVTLIINDDVALAAETGADGVHLGRDDAAFAAARRRL
                     GARALIGVSCYDSLERARAACAAGADYVAFGAFYPSAIKPDAVRAPLSLLGEARHALA
                     RRIPIVAIGGIDAGNGAGL"
     gene            complement(530..1339)
                     /locus_tag="PLQ18_12970"
     CDS             complement(530..1339)
                     /locus_tag="PLQ18_12970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008931067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HNE02612.1"
                     /translation="MSQSYTVPVVMTLAGNDPSGGAGLQADIEAIASMGCHAAPVVTA
                     LTVQDTCDVLAVVPIDPDLVYAQARTVLNDAPVAAIKIGLLGSPANAEIIAELLEDYP
                     HIPLILDPVLAAGGGRDFDGDALADAIRRHLLARTTVLTPNSVEARRLAPAADTLDAC
                     AQALLDTGCAHVLITGTHETTDAVVNRLYGDGRLLDSWTWERLPHDFHGSGCTLAAAL
                     AGLLAQGNELHAAVAQAQEYTWEALRGGYRIGAGQRIPNRLFWADEDGADD"
     gene            1460..1630
                     /locus_tag="PLQ18_12975"
     CDS             1460..1630
                     /locus_tag="PLQ18_12975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008173532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HNE02613.1"
                     /translation="MKQYMCLICGWIYDEALGWPEEGIPPGTAWADVPEDWKCPDCGA
                     GKSEFEMIEIGD"
BASE COUNT          227 a          574 c          677 g          239 t
ORIGIN      
        1 caggccggcg ccgttgccgg cgtcgatgcc gccgatcgcg acgatcggga tgcggcgcgc
       61 gagcgcatgg cgcgcctcgc cgagcagcga cagcggcgcg cgcacggcgt cgggcttgat
      121 cgcggacgga taaaaggcgc cgaaggccac gtagtcggcg ccggctgcgc aggctgcgcg
      181 ggcacgctcg aggctgtcgt agcaggacac gccgatcagc gcccgcgctc cgagccggcg
      241 ccgtgccgcg gcgaaggcgg cgtcgtcacg gccgaggtgc acgccgtcgg cgccggtctc
      301 ggcggcgagg gccacgtcgt cgttgatgat cagcgtcacg gcgcgggcgc ggcagagtcc
      361 ggcgagtgcc gcggcgagct gccggcgccg cgcggcatcg gcgcgcttgt cgcggtactg
      421 gatcgtgcgt gcgccgccgt cgatggccgc cgagacccgt tcgaggagct gcccgtccgg
      481 gatcaggccg ggatcggtga tcgcgtacag gccgtgcggg cgcggccgtt cagtcatcgg
      541 cgccgtcctc gtcggcccag aacagccggt tcgggatgcg ctggccggcg ccgatgcggt
      601 agccgccgcg cagcgcctcc caggtgtatt cctgcgcctg cgcgaccgcg gcgtgcagct
      661 cgttgccctg cgcgagcagg ccggcgagcg cggcggccag cgtgcagccg gagccgtgga
      721 agtcgtgcgg caggcgctcc caggtccagc tgtcgagcag gcggccgtcg ccgtagaggc
      781 ggttgacgac ggcatcggtg gtctcgtgcg tgccggtgat cagcacgtgc gcacagccgg
      841 tgtcgagcag cgcctgcgcg caggcatcga gggtgtcggc ggccggggcg agccggcggg
      901 cctcgacgct gttcggggtc agcaccgtcg tgcgcgccag cagatgccgg cggatcgcat
      961 cggcgagcgc atcgccgtcg aaatcgcgcc cgccgccggc ggcgagcacc gggtcgagga
     1021 tcagcgggat gtgcggatag tcttcgagca gctcggcgat gatctcggcg ttggccggcg
     1081 agccgagcag gccgatcttg atcgctgcga ccggcgcgtc attcagcacc gtgcgcgcct
     1141 gcgcgtagac gaggtcggga tcgatcggca ccacggcgag cacgtcgcag gtgtcctgga
     1201 cggtcagcgc cgtcaccacc ggcgctgcgt ggcagcccat gctggcgatg gcctcgatgt
     1261 cggcctgcag gccggcgcca ccgctcgggt cgttgccggc cagcgtcatg accaccggta
     1321 ccgtgtagct ctggctcatc gcgggcgtcc tgaaggcttg aagcagggga gctggccatg
     1381 tcgaagtgcg cggcgtcccc gcacccggac gttttgcggc acgataaccg atccttaccg
     1441 ccctcgcgag gtagtgcccg tgaagcagta catgtgcctg atctgcggct ggatttacga
     1501 cgaggcgctg ggctggcccg aggaaggcat cccgccgggc acggcctggg ccgacgtgcc
     1561 cgaggactgg aagtgtccgg actgcggcgc gggcaagtcc gaattcgaga tgatcgagat
     1621 cggcgactga aggcaggccg gggcggtgtg cgcaccgccc cggcgcaggt tcaggccgcg
     1681 gccgatgcgg ctggcgacgc cggcggcgat ctgggta
//
LOCUS       DAOQJH010000661         1710 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_62597_length_1710_cov_4.090030, whole genome shotgun sequence.
ACCESSION   DAOQJH010000661 DAOQJH010000000
VERSION     DAOQJH010000661.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1710)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1710)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1710
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62597_length_1710_cov_4.090030"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..364
                     /locus_tag="PLQ18_12980"
     CDS             <1..364
                     /locus_tag="PLQ18_12980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015719624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="modification methylase PaeR7I"
                     /protein_id="HNE02614.1"
                     /translation="PDIKGSAHVVHEPGRLYPHHNLYYVTATAWDLHALQAVLLSGLT
                     RAFIASYSTRMRGGYLRFQAQYLRRLRLPYWHELAGPLRQELIHAARSRDLAACDRAV
                     AQLYGLSAAEQAELLAQR"
     gene            365..1111
                     /locus_tag="PLQ18_12985"
     CDS             365..1111
                     /locus_tag="PLQ18_12985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017173568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaeR7I family type II restriction endonuclease"
                     /protein_id="HNE02615.1"
                     /translation="MALDLADYELRATAAVQAFWNSRETARQRQQAAGLLDAGERAGV
                     TAGRNMDGFCGLIVALAHANGLDAACIHRARRLLTLPGYFRPTKQWDLLIVDPHDGAL
                     IAAIELKSQVGPSFGNNFNNRTEEAIGTAHDFWTAYREGALGATLRPFTGWLMLVEDA
                     PGSRRPVRDASPHFPVLPEFAAASYLRRYELLCERLVREQLYTAAAVIASPRSAAGDG
                     QWTELSPATGLRTFVSALAGHMAAVAAQRG"
BASE COUNT          274 a          598 c          564 g          274 t
ORIGIN      
        1 cccggacatc aagggctcgg cccacgtcgt ccatgaaccc ggccgcctgt atccgcatca
       61 caatctgtat tacgtcacgg ccacggcctg ggacctgcac gcgctgcagg ccgtgctgct
      121 gtcggggctg acccgggcct tcatcgccag ctactcgacg cggatgcgtg gcggttatct
      181 gcggtttcag gcccagtacc tgcgccggct gcgactgccg tactggcacg agctggccgg
      241 accgctgcgt caggaactga tccatgccgc ccgcagccgc gacctcgccg cctgcgatcg
      301 ggccgtggcg cagctctatg ggctgagcgc agccgaacag gcagaactgc tggcgcaacg
      361 ctgaatggca ctcgatctgg ccgattacga actgcgggcc acagccgcgg tgcaggcgtt
      421 ctggaacagc cgcgagaccg cacgccagcg ccagcaggcg gccggcctcc tcgatgcggg
      481 cgagcgcgcc ggcgtcaccg ccggcaggaa catggatggc ttctgcgggc tgatcgtcgc
      541 gctggcacac gcgaacgggc tcgacgcggc atgcatccat cgcgcacggc gcctgctgac
      601 gctgcccggc tacttccggc cgaccaagca gtgggatctg ctgatcgtcg atccgcacga
      661 tggcgccctg atcgccgcga tcgaactgaa aagtcaggtc ggaccatcgt tcggcaacaa
      721 cttcaacaac cgtaccgaag aagccatcgg taccgcgcac gacttctgga cggcataccg
      781 cgaaggcgca ctcggggcca cgctgcgccc gttcaccggc tggctgatgc tggtcgagga
      841 cgcgcccggc tcgcgtcgtc cggtgcggga cgcatcgccg catttcccgg tgctgccgga
      901 atttgccgcc gcttcctacc tgcggcgcta cgagctgctg tgcgagcgtc tggtccgcga
      961 acagctctac accgccgctg cggtgatcgc ctcgccacgc agcgccgccg gagatgggca
     1021 gtggacggaa ctgtcaccgg ccaccgggct gcgaaccttc gtcagcgccc tggccggcca
     1081 catggcggcg gttgccgcgc agcgcggctg aaaccgatca ggcccgttcg gaccacgtat
     1141 tcagccagca ggccggcggc atccgtctga acctgtcggg acgaatggcc caccggagcg
     1201 gcggtgcgcc gcttctgctt ctgcatgctc gctgccggtt cggcggctgg cagaagcaag
     1261 ccgtctgcac gcgcaccgcg cggctttcag cgcagcgtga cctgattgcc gctgccggtg
     1321 atctgcgtcg agacgttgcc gtggccgtcg gtctttgcgt gctgcccggt cattgcgctg
     1381 cctccatgcg aggggtgccc gaccgatcat atgactgaat ggcggaaaaa cacgccggcc
     1441 gggccgatgc ggtttcacgt ggaacacctg ccgcacaggc cgctgacgaa ggaaacccgg
     1501 cgatcacgct ttccggccgc ctcagcagac gtaggggaac cggtcagggt ccggaagcta
     1561 accggtcagg tccacgacct gacccggttg tgctccggag ggaactggtt aagtccgcag
     1621 cagaacgggt ttcccttcaa cggaaaccgg cctggggttt gtacctggcc gattgccgcc
     1681 gtagcccagc cgccgaccgc cgccgcaccg
//
LOCUS       DAOQJH010000662         1710 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_62617_length_1710_cov_3.149849, whole genome shotgun sequence.
ACCESSION   DAOQJH010000662 DAOQJH010000000
VERSION     DAOQJH010000662.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1710)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1710)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1710
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62617_length_1710_cov_3.149849"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(178..1023)
                     /gene="ttcA"
                     /locus_tag="PLQ18_12990"
                     /pseudo
     CDS             complement(178..1023)
                     /gene="ttcA"
                     /locus_tag="PLQ18_12990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020647957.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
     gene            1190..1618
                     /locus_tag="PLQ18_12995"
     CDS             1190..1618
                     /locus_tag="PLQ18_12995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004999689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4112 domain-containing protein"
                     /protein_id="HNE02616.1"
                     /translation="MPSTYEAGPDGRGWHAVDERPRAAADPVLEARLRELRQLAWLLD
                     ARFRIPGTGWRIGLDGLIGVIPGIGDGLPMLAGAWIIWRSAQLGVPQTTLLRMVGNLL
                     IDAAAGSVPVLGDVFDVPFKANLRNLDLLHEHFGLPPMRR"
BASE COUNT          255 a          608 c          583 g          264 t
ORIGIN      
        1 gagacggcgg cttcagccgg ccccgatcgg gatgccgcgg aacatgcgca cgccgcccgg
       61 cccgccggca tcgccgctgc cggcgccgat gcgcggctcg ccgccgtcgc catcgccgcc
      121 gtcggcggcg gccggatcgt cccgatcctc agccgcgtcg tcgccagttt cccgcgccgg
      181 caccagccag tcgcggcgcg gcgcggccgt ggcaccgaga ccggcgaagt cgaacagccc
      241 ggcatcggcg agctgcgagg gcgcgacgtt cttcacggcc gagaacatgc tctccagccg
      301 ccccggatgg cgcctgtccc agtcgcgcag catgtcgccg atcaccgcgc gctgcaggtt
      361 cggctgcgag ccgcacagat tgcacgggat gatcgggaag cccttcgcgg ccgcgtagcg
      421 ctcgatgtcc ttctccttgc agtacgcgag cggccggatc acgacgttgc ggccgtcgtc
      481 ggacctgagc ttcggcggca tcgccttcag gctgccgccg tagaacaggt tcagcagcag
      541 ggtttcgagg atgtcgtcgc ggtggtggcc gagcgcgatc ttgctgaagc cgtgcgtgtg
      601 cgcgtaggtg tacagaatcc cgcgccgcag ccgcgagcac agcgagcacg tcgtcttgcc
      661 ctcgggcacg acgcgctgca cgacgctgta ggtgtcctgc tcgatgatct cgaacggctg
      721 gccgatcgag gcgaggtaat ccggcagcac gttcgccgga aaacccggct gcttctggtc
      781 gaggttgacg gcgaacagct cgaagcgcac cggcgccgac tgctgcagcg agcgcaggat
      841 gtcgagcatc gtgtacgaat ccttgccgcc ggacaggcag accagcacgc ggtcgccgtc
      901 ctcgatcatg ttgtagtcct ggatcgcctc gccgacgccg cgacgcaggc gcttggcgag
      961 cttgacgagg ttcacgcgct cgcgttcgga gcgcggggac ggcacggcgg ctggctggtt
     1021 catcacgaac ggtccggaag tggcgcgcga cccgcacccg ggccgcgaaa ggcgcggaat
     1081 gctagcaggc acacgggctt gtcggtatcg cgccggctgc ttccggaaca ccggcggccg
     1141 gaagcagtcg ataaccggac cctcacctgt aatgaacgga gcctcctcca tgccgagcac
     1201 ctacgaagcc ggacccgatg gccgcggctg gcacgccgtc gacgaacgtc ctcgcgccgc
     1261 cgccgatccc gtgctcgaag cccggctgcg cgagctgcgt cagctcgcct ggctgctcga
     1321 tgcgcgcttc cgcatccccg gcaccggctg gcgcatcggc ctcgacggac tgatcggcgt
     1381 catccccggc atcggtgacg gcttgccgat gctcgccggg gcatggatca tttggcgcag
     1441 tgcacaactc ggggtgccgc agacgacgct gctgcgcatg gtcggcaacc tgctgatcga
     1501 cgccgccgcc ggcagcgtgc cggtgctcgg cgatgtcttc gacgtcccct tcaaggccaa
     1561 tctgcgcaac ctcgacctgc tgcacgaaca cttcggcctg cccccgatgc gccgctgaat
     1621 cctgccggaa caggccggat ccgaccggct cccgcgggat tcaggccgct gaattttcgg
     1681 acaggcggga accccgtcgt tgacgggtct
//
LOCUS       DAOQJH010000663         1709 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_62689_length_1709_cov_3.680169, whole genome shotgun sequence.
ACCESSION   DAOQJH010000663 DAOQJH010000000
VERSION     DAOQJH010000663.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1709)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1709)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1709
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62689_length_1709_cov_3.680169"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            83..784
                     /locus_tag="PLQ18_13000"
     CDS             83..784
                     /locus_tag="PLQ18_13000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014198774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNE02617.1"
                     /translation="MMSRPPLLLIPGLLCDGRLWQHQLAALADAADMTVGDHSTHDNL
                     YEIAATLLTKAPPRFAVAGLSMGGYIAFELLRQAPERITHLALINTAARPDSPEKRQR
                     RLDFMALATRGRFRGVSHALLPLLIHRDRLDDRELAGTVSAMARDSGVEAYLRESRAI
                     VDRPDSVPMLSGIACPTVVIGGLQDQLTPPECQQEIADGIPGAELILLDRCGHLAPLE
                     HPQAVNAALRKLLTR"
     gene            complement(885..1334)
                     /locus_tag="PLQ18_13005"
     CDS             complement(885..1334)
                     /locus_tag="PLQ18_13005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014239792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC/Spx/MgsR family protein"
                     /protein_id="HNE02618.1"
                     /translation="MAHVVFFEKPGCGGNARQKAALRAAGHSLEVRDLLTWPWTAAQL
                     QEWFSGLPVADWFNRSAPKVRDGEILPERLSAEAALALMLAEPLLIRRPLLEAAGEKR
                     VGWDQEAVDAWLGLAANAATVGEGCPKGEKMAPCPAPVPGTAADTAQ"
BASE COUNT          245 a          610 c          586 g          268 t
ORIGIN      
        1 aggcccacgg cctgcaggcc ccgtgcaacc gcacgctgac cgagctgatc cgcctgcgca
       61 ccgccaccgc cggaggtgca gcatgatgtc ccgcccgccg ctcttgctga ttcccggcct
      121 gctctgcgac ggccggctgt ggcagcacca gctcgccgcg ctcgccgatg ccgccgacat
      181 gacggtcggc gatcactcga cgcacgacaa cctctacgag atcgccgcca cgctgctgac
      241 caaggcgccg ccgcgtttcg cggtcgccgg gctgtcgatg ggcggttaca tcgcctttga
      301 actgctgcgc caggcgccgg agcgcatcac gcatctggcg ctgatcaata ccgccgcgcg
      361 cccggactcg cccgaaaagc gccagcggcg gctggatttc atggcgctcg ccacccgcgg
      421 acgtttccgc ggcgtcagtc acgcactgct gccgctcttg atccaccgcg accggctcga
      481 tgaccgcgag cttgccggca cggtcagcgc gatggcgcgc gattcgggcg tggaagccta
      541 tctgcgcgaa agccgcgcca tcgtcgatcg ccccgacagc gtgccgatgc tctccggcat
      601 cgcctgcccg accgtcgtca tcggcggcct gcaggatcag ctgacgccgc ccgagtgcca
      661 gcaggaaatc gccgacggca ttcccggggc cgagctgatc ctgctcgatc gctgcggcca
      721 tctggcgccg ctcgaacacc cgcaggcggt caacgccgcg ctgcgcaagc tgctgacacg
      781 ctgacgccgc gcccccgccc gcctgtcgtt caagcccctc gcccctcgcg gcagagggct
      841 tgggtggagg ggggaaaaaa ccatgcattt ccgggtgatc gatatcactg cgccgtatcg
      901 gccgccgtgc ccggcaccgg cgccgggcac ggcgccatct tctcgccctt cgggcagcct
      961 tcgccgaccg tcgcggcatt ggcggcgagg ccgagccagg catcgaccgc ctcctgatcc
     1021 cagccgacgc gcttctcgcc tgcagcttcg agcagcgggc ggcggatcag cagcggttcg
     1081 gcgagcatca gtgccagtgc ggcttcggcg ctcagccgct cgggcaggat ttcaccgtcg
     1141 cgcaccttcg gtgccgagcg gttgaaccag tcggcgaccg gcaggccgct gaaccattcc
     1201 tgcagctggg cggccgtcca cggccaggtg agcaggtcgc gcacttcgag gctgtggccg
     1261 gcggcgcgca gggcggcttt ctgcctggcg ttgccgccgc agccgggttt ttcaaagaag
     1321 acgacgtggg ccatcagccg gactctccgt gcaggcgttc gcaggatcac cttgtcgcaa
     1381 acacgacaaa ccggccgcgg aatccgggcc gtggcggggg tggagcagga tgcgtgccgg
     1441 cgatggcgcc cgaggcccgc ggcgatcggg cgcaggggca gcggtgccgg atcggtcggt
     1501 gcccttgtcc gggtgagtga gtcgcgaagg gcggacggga gggggtgcgg gcgggatcag
     1561 cccttgcgca gcttgatgtc gtaataactc cggatgatgc cctggtcgtc cggccagtcc
     1621 gcatacgctt tccaggcacc accggctttc ggcatctggc gggtgtggac acgtacctga
     1681 ctgcggtcgg cagcgatttc gagcaggtt
//
LOCUS       DAOQJH010000664         1708 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_62758_length_1708_cov_5.134906, whole genome shotgun sequence.
ACCESSION   DAOQJH010000664 DAOQJH010000000
VERSION     DAOQJH010000664.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1708
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62758_length_1708_cov_5.134906"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..375)
                     /locus_tag="PLQ18_13010"
     CDS             complement(<1..375)
                     /locus_tag="PLQ18_13010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019398191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNE02619.1"
                     /translation="MTASTRSAPGYWSIALAAAAILAITMGARQTLGLFVAPLDASTG
                     LGVASISFALAIGQFVWGASQPVFGALADHYGPGRALVAGGVLLALGMALTPFMQSEP
                     GLILTLGVLAAAGAGAGSFSILI"
     gene            874..1254
                     /gene="gcvH"
                     /locus_tag="PLQ18_13015"
     CDS             874..1254
                     /gene="gcvH"
                     /locus_tag="PLQ18_13015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015752004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HNE02620.1"
                     /translation="MNPSELLYTKEHEWLRAEDDGSYTLGVTEYAQKQLGDIVFCEQP
                     VIGAKFEAEEPVGTLESVKAVAEVYVPVGCEITAINEALNDSPELVNEDPYGEGWLFR
                     LKATAAPEGLLSADAYEQYLQSAE"
     gene            1343..>1708
                     /gene="tpx"
                     /locus_tag="PLQ18_13020"
     CDS             1343..>1708
                     /gene="tpx"
                     /locus_tag="PLQ18_13020"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012970133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HNE02621.1"
                     /translation="MATITLRGNPIHTNGELPAAGTRAPDFHLVAPGLADVHLENYAG
                     KKKLLNIVPSLDTPTCQLSTKKFNDHAKAHPDTVILIVSADLPFAQGRFCTGEALDNV
                     IPLSMMRSRNFAKDYGVLIE"
BASE COUNT          299 a          616 c          550 g          243 t
ORIGIN      
        1 tatcaggatc gagaagctgc cggccccggc tccggccgcg gccagcacgc cgagcgtcag
       61 gatcaggccc ggctcgctct gcatgaacgg cgtcagcgcc atgccgagcg cgagcagcac
      121 gccgccggcc accagcgccc ggcccggccc gtagtgatcg gccagcgccc cgaacaccgg
      181 ctgcgaggcg ccccagacga actggccgat cgccagcgcg aagctgatcg acgcgacccc
      241 gagcccggtc gaggcatcga gcggggcgac gaacaggccg agcgtctggc gcgcgcccat
      301 cgtgatcgcg aggatcgcgg cggcggcgag cgcgatgctc cagtagccgg gggcggagcg
      361 ggtggaagcg gtcacgggcg ggtcctcgcg ggcagacggc gggcagcggt gcgcgccacg
      421 ggctgcaccg gacagatcag acggattcgg gtgccgcact atggaaatcc gccggcagcc
      481 tgaccagccg ggcgcagcga tggctgccat gcacgcacga agcgctgccg gcgtgatccg
      541 gcgttgatcc atgcttgccg gatcaatcga gaatcattat catttgtcct gagttcgcga
      601 cacggtggca ggcaccgcgc ggcgcgctca tctctctcta cctcattgag ggcgtgcgtc
      661 ggcggacgca cgcccttctt ttttgtcatt cgccaggcct ccgccccgtc attcattcgt
      721 catccgcgcg cccgtttttc agtccggcgc acgctggcag cccctgctgc gtctgcatat
      781 agtctcggcc tgccggcttt ggccggaccc cgcccgcctt ccgacccttc ccgtcatccc
      841 cgccgcgccc ccgcgcacag gagagtgtcc gccatgaacc ccagcgaact gctgtacacg
      901 aaggaacatg aatggctgcg cgccgaagac gacggcagct atacgctcgg cgttaccgaa
      961 tatgcacaga agcagctcgg cgacatcgtg ttctgtgaac agccggtgat cggagcgaag
     1021 ttcgaggccg aggaaccggt cggcacgctc gaatccgtga aggccgtcgc cgaggtgtac
     1081 gtgcccgtcg gctgcgagat caccgccatc aacgaggccc tgaacgacag ccccgaactg
     1141 gtcaacgagg acccgtacgg cgagggctgg ctgttccgtc tcaaggcgac ggcggccccc
     1201 gaggggctgc tgagcgccga tgcctacgag cagtacctcc agagcgccga ataagccgct
     1261 gcccgtaccg gccgcagagc cggagtcgcc gcccgcccgc cgggcggcac atcacctcac
     1321 ccaccatcag gagacgccgc tcatggccac gatcacgctg cggggcaacc cgatccacac
     1381 caacggcgag ctgccggccg ccggcacgcg cgcaccggac ttccacctgg tcgctcccgg
     1441 cctcgccgac gtgcatctcg aaaactacgc cggcaagaag aagctgctga acatcgtgcc
     1501 gagcctcgac acgccgacct gccagctgtc gaccaagaag ttcaacgacc acgccaaggc
     1561 gcatccggac accgtgatcc tgatcgtctc ggccgacctg ccgttcgcgc aggggcgctt
     1621 ctgcaccggc gaggcgctcg acaacgtcat cccgctgtcg atgatgcgtt cgcgcaactt
     1681 cgccaaggac tacggggtgc tgatcgag
//
LOCUS       DAOQJH010000665         1703 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_63130_length_1703_cov_3.764563, whole genome shotgun sequence.
ACCESSION   DAOQJH010000665 DAOQJH010000000
VERSION     DAOQJH010000665.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1703)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1703)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1703
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63130_length_1703_cov_3.764563"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..456)
                     /locus_tag="PLQ18_13025"
     CDS             complement(<1..456)
                     /locus_tag="PLQ18_13025"
                     /inference="COORDINATES: protein motif:HMM:NF023177.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3305 domain-containing protein"
                     /protein_id="HNE02622.1"
                     /translation="MTASDPGSLPEGAGTAADALPARVPVAVLMERVPVSGNRWIDHR
                     WQCAAVLVGDPASASAAPAIIDDGHGRELRIWRGLTLQLWPDEAESYRHNLDGTQPGL
                     FVATRPGADGAPEPFLVSACGDHASALEEMSEAVHRVALPPGLMPWLEAW"
     gene            672..1577
                     /locus_tag="PLQ18_13030"
     CDS             672..1577
                     /locus_tag="PLQ18_13030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006746168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HNE02623.1"
                     /translation="MATPPARRPLLSHAGRPAQYPITVVDESGTARDIHIPMERALTL
                     YVDRRELVTLMTLGLEPEWLALGYLRTQGLVDALDDIAAVQVDWEVEAVAVRTRQGIE
                     DWDARLGVRTVTTGCGQGTMFGRLSERAVSVRVEAPVLRQSLLYALGERLRLRDTLYK
                     HTGSIHGCALCAGDEVLCFVEDVGRHNAVDAIAGWMWLEGVEGAGKLFYTTGRLTSEM
                     VLKTAQMGVPVLVSRSGTTQAAVELARRIGVTLIGRLRNRHFFVYHGAERVRFDAVPP
                     ALPRTQGGKEHLPADEAALSPEGRP"
BASE COUNT          235 a          615 c          614 g          239 t
ORIGIN      
        1 ccacgcctcc agccacggca tgagcccggg cggcagcgcg acgcgatgca cggcctcgct
       61 catctcctcc agcgcgctgg cgtgatcgcc gcaggcgctg accagaaacg gctcgggcgc
      121 gccgtccgcg ccggggcggg tcgcaacgaa caggcccggc tgcgtgccat cgaggttgtg
      181 gcggtagctt tcggcctcgt ccggccacag ctgcagcgtg agcccccgcc agatgcgcag
      241 ctcgcggcca tggccatcgt cgatgatcgc cggtgccgcg ctggcgctgg ccgggtcacc
      301 gaccagcacg gccgcgcact gccagcgatg atcgatccag cggttgccgc tgaccggcac
      361 gcgctccatc agcacggcga ccggcacgcg ggccggcagg gcatcggcag cggtccccgc
      421 gccttcgggc agggaaccgg gatcggaggc agtcatggcg gcagccctcg gtggcaggac
      481 cgccaacgat acgcaatcgc cgttgcggcg ccagtcgcag cagtgcagac cggatcgggc
      541 aggaatcgtg ctaatcatgg ccgcccgcag ccggcggcct tccggatcgc gtcactttga
      601 cccggcagat ttgcatctgc cggggcggga agatgcactc tgtccgcctt cagcccttgc
      661 ggaagcgttt catggccacg ccccctgccc gccgcccgct gctgagccac gccggccggc
      721 ccgcgcaata cccgatcacc gtcgtcgacg agagcggcac ggcgcgggac atccacatcc
      781 cgatggaacg cgcgctgacg ctgtacgtgg accggcgcga gctggtgacg ctgatgacgc
      841 tcggcctcga accggagtgg ctggcgctcg gctacctgcg cacgcagggc ctcgtcgatg
      901 cgctcgatga catcgccgcc gtgcaggtcg actgggaggt cgaggccgtg gccgtgcgca
      961 cgcgccaggg catcgaggac tgggatgcgc gcctcggcgt gcgcaccgtc accaccggct
     1021 gcggccaggg cacgatgttc ggccgcctca gcgagcgggc cgtttcggtc cgcgtcgagg
     1081 cgcccgtgct gcgccagtcg ctgctgtacg cgctcggcga gcggctgcgg ctgcgcgaca
     1141 cgctgtacaa gcacaccggc tcgatccacg gctgcgcgct ctgcgccggt gacgaggtgc
     1201 tctgcttcgt cgaggacgtc ggccgccaca acgccgtcga tgcgattgcc ggctggatgt
     1261 ggctcgaagg cgtcgagggc gccggcaagc tgttctacac caccgggcgg ctgacctcgg
     1321 agatggtgct gaagaccgcg cagatgggcg tgccggtgct ggtgtcgcgc tcgggcacga
     1381 cgcaggcggc ggtcgaactg gcgcggcgca tcggcgtgac gctgatcggc cggctgcgca
     1441 accgccactt cttcgtctat cacggcgccg aacgcgtgcg gttcgacgcc gtgccgcccg
     1501 cgctgccgcg cacgcagggt ggcaaggaac acctgccggc cgatgaggcc gcactgtccc
     1561 cggagggccg cccatgagca ctgatccctt cctgcgccgg ctgctgcgca ccgtcgccga
     1621 cgacggcagt gccggtttcg gacggcgcgc gcagccgggg gaatgggccg tgcccggcgc
     1681 ctgtgcattc gccgccgagg acc
//
LOCUS       DAOQJH010000666         1702 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_63205_length_1702_cov_4.151184, whole genome shotgun sequence.
ACCESSION   DAOQJH010000666 DAOQJH010000000
VERSION     DAOQJH010000666.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1702)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1702)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1702
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63205_length_1702_cov_4.151184"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..392)
                     /locus_tag="PLQ18_13035"
     CDS             complement(<1..392)
                     /locus_tag="PLQ18_13035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012639491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HNE02624.1"
                     /translation="MEFKDYYQILGVSRTAAEDEIKKAYRRLARKYHPDVSKEAHAEA
                     RFKEVAEAWEVLRDPEKRADYDRLGPNFRNGQDFRPPPGWRPHAGAGAHRGGGPSAAD
                     GFSQADFSEFFESIFGGGGGAQGVGARA"
     gene            complement(510..956)
                     /locus_tag="PLQ18_13040"
     CDS             complement(510..956)
                     /locus_tag="PLQ18_13040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018141215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HNE02625.1"
                     /translation="MSSNRYDPWTRLGLLTKELERVFDPRLANNEDASNVATCDWVPA
                     VDIKEEAGRFLIHADIPGVDPKDIDITMENGVLTLRGQRSSERREAADGWKRVERTHG
                     SFYRRFGLPDTADASRISARGQNGVLEIVIPKQERAAPRKITVSAE"
     gene            1169..>1702
                     /gene="moaB"
                     /locus_tag="PLQ18_13045"
     CDS             1169..>1702
                     /gene="moaB"
                     /locus_tag="PLQ18_13045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008182042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HNE02626.1"
                     /translation="MSEARQFQPLSIAVLTVSDTRGEAEDVSGRTLVERLEAAGHRLA
                     EKAMCRDDLYAIRAIVSRWIADPAVQVVISTGGTGVTGRDGTPEAVQVLLDKEISGFG
                     ELFRMLSWEEIRTSTIQSRALAGVANGTYLFVLPGSSGACRTAWDRILKDQLDYRHRP
                     CNLVELMPRLQEHLAKKS"
BASE COUNT          230 a          604 c          588 g          280 t
ORIGIN      
        1 tgggcgcggg caccgacgcc ctgcgcgccg ccgccgccgc cgaagatgct ctcgaagaac
       61 tcgctgaagt cggcctgcga gaagccgtcg gccgcgctcg gcccgccgcc ccggtgcgcg
      121 ccggcaccgg cgtgcggacg ccagcccggc ggcgggcgga agtcctggcc gttgcggaag
      181 ttcggcccga gccggtcgta gtccgcgcgc ttttcgggat cgcgcaggac ttcccaggct
      241 tcggcgacct ccttgaagcg cgcctcggca tgggcttcct tgctgacgtc cgggtggtac
      301 ttgcgcgcga gccgccggta ggccttcttg atctcgtcct cggccgccgt gcgcgacacg
      361 ccgaggatct ggtagtagtc cttgaattcc ataccgttta ccgcactgcg ggccgggccg
      421 ctgaagcgac ccggcccggc gtggagactc gggggcacgc cccccgccgt gcgggcgggg
      481 ggcgcgggct gcgcgatgcg ctcagggcgt cattcggcgc tgaccgtgat cttgcgcggt
      541 gccgcgcgtt cctgcttcgg aatcacgatc tccagcacgc cgttctggcc gcgggcgctg
      601 atgcgcgagg catcggcggt gtccggcagg ccgaagcggc ggtagaagct gccgtgcgtg
      661 cgctcgacgc gcttccagcc atcggccgcc tcgcgccgct cggagctgcg ctggcccctg
      721 agcgtcagca cgccgttttc catcgtgatg tcgatgtcct tcggatcgac accggggatg
      781 tcggcatgga tcaggaagcg cccggcttcc tccttgatgt cgacggccgg cacccagtcg
      841 caggtcgcga cgttggaggc atcctcgttg ttggccagac gcggatcgaa cacgcgctcc
      901 agctccttgg tcagcaggcc cagacgggtc cacgggtcgt agcggttgct gctgatgctc
      961 atgttgcacc tcggaaaatc gctgcgttgt gtcgggggga agcggtcatc gcgaccgccg
     1021 tgatccgtta gataaggtcg ggccggaggc tttcaagagc ggcggattca ggccggcatg
     1081 cgccggatca tcaccggcgc actggccgcc ggcggtccga tcgggtatct agccgcgact
     1141 gcccctgccc ccgccgagga cctcgccgat gagcgaagcg cgccagttcc agcccctgtc
     1201 gatcgccgtg ctgaccgttt ccgacacgcg cggcgaagcc gaggatgtct ccggccgcac
     1261 gctggtcgag cgcctcgaag ccgccggtca ccggctcgcc gaaaaggcga tgtgccgcga
     1321 tgacctttat gcgatccgcg ccatcgtctc gcgctggatc gccgatccgg ccgtgcaggt
     1381 cgtcatcagc accggcggca ccggcgtcac cggccgcgac ggcacgcccg aggccgtgca
     1441 ggtgctgctc gacaaggaaa tctccggctt cggcgagctg ttccgcatgc tgtcctggga
     1501 ggagatccgc acctcgacca tccagtcgcg cgcgctcgcc ggggtcgcca acggcaccta
     1561 cctgttcgtg ctgcccggct ccagcggcgc ctgccgcacg gcctgggacc gcatcctcaa
     1621 ggaccagctc gactaccggc accggccgtg caacctcgtc gagctgatgc cgcgcctgca
     1681 agagcacctc gcgaagaagt cg
//
LOCUS       DAOQJH010000667         1702 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_63228_length_1702_cov_2.901032, whole genome shotgun sequence.
ACCESSION   DAOQJH010000667 DAOQJH010000000
VERSION     DAOQJH010000667.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1702)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1702)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1702
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63228_length_1702_cov_2.901032"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..530
                     /locus_tag="PLQ18_13050"
     CDS             <1..530
                     /locus_tag="PLQ18_13050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002709530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HNE02627.1"
                     /translation="RTVEKSYLAVVRGWPAETGTIDYALAEEPGKPLQPAITHWRRLA
                     TAEIPVPVGRYAQARCALLEVRPETGRLHQIRKHFHHVSHHLIGDTTHGDGRHNRLFR
                     ERFGCRRLLLHAWRLALPHPLDGTPLELIAPPDAELAQLFDALGGSPGNGPGAGLRQD
                     QGLAGWQASIPYGCV"
     gene            677..958
                     /locus_tag="PLQ18_13055"
     CDS             677..958
                     /locus_tag="PLQ18_13055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02628.1"
                     /translation="MRTTTAIALGFSATLGLAALAQAGDFEQYPPTDGWGRPVAAADA
                     ALQESAEMQIRMMPGYAQGDYLPAEGPTCDLAQTLFPVHSTEAGSAGLC"
     gene            complement(1070..>1702)
                     /locus_tag="PLQ18_13060"
     CDS             complement(1070..>1702)
                     /locus_tag="PLQ18_13060"
                     /inference="COORDINATES: protein motif:HMM:NF024841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HNE02629.1"
                     /translation="DDFSATNQGRSIGISSITALNDHEFLVIERDNRGIGEADPTGTA
                     PVGSKRVYRIDISGASDVSSTSLAGDNALPPGVVPVTKSLFLDVQAALLAAGLPIPEK
                     LEGLTIGPLLDNGFIDLILGTDNDFSVTQSGSGEQFNVCTGPGGTFRDVALDQACPGA
                     TALLPTYLYAVRVELAGLNYVAPLTVAEPFGLTLFGLGLAGLAARRRCRA"
BASE COUNT          263 a          616 c          569 g          254 t
ORIGIN      
        1 agcgcacggt tgaaaagagc tatctcgccg tcgtgcgcgg ctggccggcc gagaccggca
       61 cgatcgacta cgcgcttgcc gaggagcccg gcaaaccgct gcagccggcg atcacgcact
      121 ggcggcggct cgccacggcc gaaatcccgg tgccggtcgg ccgttacgcg caggcgcgct
      181 gcgcgctgct ggaagtgcgc cccgaaaccg gccggctgca ccagatccgc aagcacttcc
      241 atcacgtctc gcatcacctg atcggcgaca ccacgcacgg cgacggccgc cacaaccggc
      301 tgttccgcga gcgcttcggc tgccgccggc tgctgctgca cgcctggcgg ctggcactgc
      361 cgcatccgct cgacggcacg ccgctcgaac tcatcgcccc gcccgatgcg gagcttgcgc
      421 agctgttcga tgcgctcggc gggtcgcccg ggaacggacc gggggccgga ctgcggcagg
      481 atcaagggct tgccggatgg caggcatcca ttccatacgg gtgcgtttaa tggctctgct
      541 gccgataacc aagtcctgca cttggaaatg tgcagccgcg ggctacgctg cacttgcaca
      601 ttgcaagcga ttcatctttc cccgacgaat cgccggcggt gctcacactg gcaaacccgc
      661 tcaggaggtt cgagccatga ggaccacgac agccatcgcc ctcggcttct ctgccaccct
      721 cggtctggcc gcgctcgcgc aggccggcga cttcgaacag tacccgccga ccgacggctg
      781 gggccgcccg gtcgccgctg ccgacgccgc gctgcaggaa tccgccgaaa tgcagatccg
      841 gatgatgccc ggctacgcgc agggcgacta cctgccggcc gaaggcccga cctgcgatct
      901 cgcgcagacc ctgttcccgg tgcattcgac cgaagccggc agcgccggcc tctgctgacc
      961 ggggcagccg ccccgcggcg ccggctgtcc gccgcgggac ggtgttcttc tcacaaacct
     1021 gtccggcagc cctgcacgaa cggcgtcccg gtgacgccgt tccgcgtttt caggcccggc
     1081 accggcgacg ggccgcgagc ccggccaggc cgagcccgaa cagcgtcagc ccgaacggtt
     1141 ccgccaccgt cagcggcgcc acgtagttga ggcccgcgag ttcgacgcgc accgcgtaca
     1201 ggtacgtcgg cagcagcgcc gtagcgcccg ggcaggcctg atcgagcgcc acgtcgcgga
     1261 aggtgccgcc gggaccggtg cagacgttga actgctcgcc cgaaccgctc tgcgtgacgc
     1321 tgaaatcgtt gtcggtgccg aggatcaggt cgatgaagcc gttgtcgagc agcggcccga
     1381 tcgtcagccc ttcgagcttc tcggggatcg gcaggccggc ggcgagcagc gcggcctgca
     1441 cgtcgaggaa cagcgacttc gtgaccggca cgacgccggg cggcagcgcg ttgtcgccgg
     1501 caaggctggt gctgctcacg tcgctcgcac cgctgatgtc gatgcggtac acacgcttgc
     1561 tcccgaccgg cgcagtcccg gtcggatcgg cctcgccgat gccgcggttg tcgcgctcga
     1621 tgaccaggaa ctcgtgatcg ttcagcgccg tgatcgagct gatgccgatc gagcggccct
     1681 gattggtcgc ggagaagtca tc
//
LOCUS       DAOQJH010000668         1701 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_63282_length_1701_cov_4.166464, whole genome shotgun sequence.
ACCESSION   DAOQJH010000668 DAOQJH010000000
VERSION     DAOQJH010000668.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1701
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63282_length_1701_cov_4.166464"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..385)
                     /locus_tag="PLQ18_13065"
     CDS             complement(<1..385)
                     /locus_tag="PLQ18_13065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005001064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide diphosphorylase
                     (carboxylating)"
                     /protein_id="HNE02630.1"
                     /translation="MNAADIAAQVRAALAEDIGSGDLTADLIPATNRAEATVIAREPA
                     VICGVAWFDEVFRQIDPSIRIDWRVRDGERVSADQLLCTLHGPSRALLSGERTALNFL
                     QTLSGTASLAKRYAEAVAGTGATVLD"
     gene            complement(420..1037)
                     /locus_tag="PLQ18_13070"
     CDS             complement(420..1037)
                     /locus_tag="PLQ18_13070"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017112913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNE02631.1"
                     /translation="MLPPLTRHHWIFDLDGTLTVAVHDFPAMRRRLGIPGHVDIIDWI
                     DTRGAAEAARLHRALDAIEHEYASLARAAEGMPELLAALETAGCRLGLLTRNTREVAG
                     QVLRRLGVDRHFAEETLFGRHEAPPKPRPDGISRLLQHWQAEAGDTVMVGDHLHDLLS
                     GRAAGTATVLISPGDPPPDWRIHIDCHFGSPGQLLAGWLAAHPPT"
     gene            complement(1046..>1701)
                     /locus_tag="PLQ18_13075"
     CDS             complement(1046..>1701)
                     /locus_tag="PLQ18_13075"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNE02632.1"
                     /translation="LGERMPLKMPDPARVDPWIDRALANNPTLRSALQAADAAKETVD
                     LQRAGHYPSLDLVASHYDNDFGPRGGDLSGNRVGIQVNIPLFQGGAVTSRTRQAAYQH
                     EAAREQVEATQRDIVRQVRNAHRGQQAAISQVKALKQALVSNRSSLDATRAGLDVGTR
                     TIVDVLDAERELYRAERDYAKARYDYVLNRLLLKQAAGDLGEADLVEINAWLQRPKS"
BASE COUNT          253 a          547 c          606 g          295 t
ORIGIN      
        1 tgtcgagcac ggtggcgccg gttccggcca cggcttcggc ataacgcttc gccaggctcg
       61 ccgtacccga cagggtctgc aggaagttca gcgccgtgcg ttcaccgctc agcagcgctc
      121 gcgacgggcc gtgcagcgtg cataacagct gatcggcact cacccgttcg ccatcccgga
      181 cccgccagtc gatgcggatc gacggatcga tctggcggaa cacctcgtcg aaccaggcca
      241 caccgcagat cacggcaggc tcgcgggcga tgaccgtggc ttcggcacga ttcgtggctg
      301 gaatcaggtc ggcggtcaga tcgccgctgc cgatgtcctc ggcaagcgca gcgcggacct
      361 gggcagcgat gtcggcggca ttcatgacgc gttcgtctcc ggggggcggc tactgctact
      421 tatgtgggag ggtgggcagc gagccagccg gcgagcagct ggcccggact gccgaagtga
      481 cagtcgatgt ggatgcgcca gtccggtggc ggatcgccgg ggctgatcag cacggttgcg
      541 gtgccggctg cacgtccgct cagcaggtca tgcaggtgat cgccgaccat gacggtatcg
      601 ccggcttcgg cctgccagtg ctgcagcagt cgggagatgc cgtccggtcg cggcttgggc
      661 ggcgcttcgt gccggccgaa caatgtttct tcggcaaaat gccgatccac accgagcctt
      721 cgcaacacct gcccggcgac ctcgcgggta ttgcgggtca gcagtcccag ccggcagccg
      781 gccgtttcca gggccgcgag cagttcgggc ataccctccg ccgcgcgtgc gaggctcgcg
      841 tattcgtgtt cgatggcatc gagggcccga tgcaggcgcg cggcttcggc tgcgccgcgg
      901 gtgtcgatcc agtcgatgat gtcgacgtgc ccgggaatgc cgagccggcg gcgcatcgcc
      961 ggaaagtcat ggaccgcaac cgtcagcgtg ccgtcgagat cgaaaatcca gtggtgacgc
     1021 gtcaggggcg gcagcacggc tcgggtcagg atttcggacg ctgcagccag gcgttgatct
     1081 cgaccagatc ggcttcgccg aggtcgccgg ctgcctgttt cagcagcagg cggttcagca
     1141 cgtagtcgta gcgtgccttg gcatagtcgc gttcggcccg gtacagctcg cgctcggcat
     1201 cgagcacgtc gacgatcgtg cgcgtgccga catcgaggcc ggcgcgcgtg gcatcgagcg
     1261 agctgcggtt cgacaccagc gcctgcttca gggccttgac ctgactgatc gctgcctgct
     1321 ggccacgatg ggcgttgcgg acctggcgga cgatgtcacg ctgcgtggcc tcgacctgct
     1381 cgcgtgccgc ttcgtgctgg taggctgcct gccgggtgcg cgaggtcacg gcaccgccct
     1441 ggaacagcgg gatattcacc tggattccga cgcggttgcc ggacagatcg ccgccgcgcg
     1501 ggccgaaatc gttgtcgtaa tggctggcga ccaggtcgag cgacggataa tggccggcac
     1561 gctgcaggtc gacggtttcc ttggcggcat cggccgcctg cagtgccgag cgcagcgtcg
     1621 gattgttggc cagggcgcgg tcgatccatg gatcgacgcg ggccggatcc ggcatcttca
     1681 gcggcatgcg ctcgccgagt t
//
LOCUS       DAOQJH010000669         1698 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_63533_length_1698_cov_2.962264, whole genome shotgun sequence.
ACCESSION   DAOQJH010000669 DAOQJH010000000
VERSION     DAOQJH010000669.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1698)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1698)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1698
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63533_length_1698_cov_2.962264"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /gene="rpsF"
                     /locus_tag="PLQ18_13080"
     CDS             complement(<1..188)
                     /gene="rpsF"
                     /locus_tag="PLQ18_13080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HNE02633.1"
                     /translation="MRHYEIVFLVHPDQSEQVPAMIDRYRSMIETDGGAIHRLEDWGR
                     RQLAYPINKVHKAHYVLM"
     gene            complement(317..1066)
                     /gene="rlmB"
                     /locus_tag="PLQ18_13085"
     CDS             complement(317..1066)
                     /gene="rlmB"
                     /locus_tag="PLQ18_13085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017764011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HNE02634.1"
                     /translation="MSEELIYGLHAVGAALRHEPQRLRGLWVDRRRRDARIRELLALA
                     EAAGVRVYAEDAEALDRQCGGERHQGVFARMKVAERQWREDELDALLDAIEGPALLLV
                     LDGVQDPHNLGACLRSADAAGVHAVLAPNDRAAGLTPTARKVACGAAEHVPFIPVTNL
                     ARTLRELKQRGIWLVGAAGEAEADLYAMDLAGPVALVLGAEEKGLRRLTREECDYLAH
                     IPMTGTVESLNVSVATGVFLFEALRQRRARR"
     gene            complement(1135..>1698)
                     /locus_tag="PLQ18_13090"
     CDS             complement(1135..>1698)
                     /locus_tag="PLQ18_13090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018140309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HNE02635.1"
                     /translation="VHHALKHLIHHGGKARGYGYSHEKMTELGEHCSMTERRADDATR
                     DAVEWLKCEYMRDKVGETFDGTVSAVTAFGLFVLLKEVFVEGLVHISNLPGDYYHFDP
                     LAHRLEGERFGRCYRLGDALRVQVARVDLDERKIDFMPADVAGVGIGLPPTPRKPAGR
                     PSRAARDDGGKPPARGGAGGPYRKRRR"
BASE COUNT          241 a          617 c          584 g          256 t
ORIGIN      
        1 ttcatcagca catagtgcgc cttgtgcacc ttgttgatcg gataggccag ctggcggcgg
       61 ccccagtcct cgagacggtg gatcgcaccg ccgtcggtct cgatcatcga ccggtaacga
      121 tcgatcatcg ccggcacctg ctcgctctgg tccgggtgga ccaggaagac gatttcgtag
      181 tgacgcatcg aagctcctta cggatgaaaa acagccgccc ggcggtccgg tgcggcaagg
      241 agtcggccct gtcggaccgg aaaagccgcg cagtgtatgg agttgtccgc gcccgggcaa
      301 gccgcggcgc cggctttcag cgccgcgcgc ggcgctggcg cagcgcctcg aacaggaaca
      361 cgccggtcgc gaccgagacg ttcaggctct cgaccgtgcc ggtcatcggg atgtgcgcca
      421 ggtagtcaca ttcctcgcgc gtcagccggc gcaggccttt ttcctcggcc ccgagcacca
      481 gcgccaccgg cccggcgaga tccatcgcat acagatcggc ctcggcctcg ccggcggcgc
      541 cgaccagcca gatgccgcgc tgcttcagct cgcgcagcgt gcgcgcgagg ttggtgaccg
      601 ggatgaacgg cacgtgctcg gccgcgccgc aggccacctt gcgcgcagtc ggcgtcaggc
      661 cggcggcgcg gtcgttcggg gcgagcaccg cgtgcacgcc ggcggcgtcg gccgagcgca
      721 ggcaggcgcc gaggttgtgc gggtcctgca cgccgtcgag caccagcagc agcgccggcc
      781 cctcgatggc atcgagcagc gcgtcgagtt cgtcctcgcg ccactggcgt tcagcgacct
      841 tcatgcgcgc gaacacgcct tggtggcgct cgccaccgca ctggcgatcg agcgcttcgg
      901 cgtcctcggc gtagacccgc acgccggcgg cttcggccag cgccagcagc tccctgatgc
      961 gcgcatcgcg ccggcgccgg tccacccaca ggccgcgcag ccgctgcggc tcgtgacgca
     1021 gcgccgcacc gaccgcatgc aggccgtaga tcagttcctc gctcatgtcg gcaggactcc
     1081 gggccgcgtg ccaacggcac gcagcggttc atcgttcagg gatcgggtcc ggcttcagcg
     1141 ccggcgcttg cggtacggcc cgcctgcgcc cccgcgcgcc ggcggcttgc cgccgtcatc
     1201 ccgggcggca cgcgacgggc gccccgccgg cttgcgcggc gtcggcggca ggccgatgcc
     1261 gacgccggcg acatcggccg gcatgaagtc gatcttgcgc tcgtcgagat cgacgcgcgc
     1321 cacctgcacg cgcagcgcat cgccgagccg gtagcagcgt ccgaagcgct cgccctccag
     1381 ccggtgggcc agcggatcga agtggtaata gtcgccgggc aggttggaga tgtgcacgag
     1441 cccctcgacg aagacctcct tcagcagcac gaacagcccg aaggccgtga ccgccgacac
     1501 cgtgccgtcg aaggtctcgc cgaccttgtc gcgcatgtat tcgcacttca gccattcgac
     1561 ggcatcgcgg gtcgcgtcgt cggcgcgccg ctcggtcatc gagcagtgct cgccgagttc
     1621 ggtcatcttc tcgtgcgagt agccgtagcc ccgggccttg ccgccgtggt ggatcaggtg
     1681 cttcagcgcg tgatgcac
//
LOCUS       DAOQJH010000670         1696 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_63664_length_1696_cov_4.187081, whole genome shotgun sequence.
ACCESSION   DAOQJH010000670 DAOQJH010000000
VERSION     DAOQJH010000670.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1696)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1696)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1696
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63664_length_1696_cov_4.187081"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            188..>1696
                     /locus_tag="PLQ18_13095"
     CDS             188..>1696
                     /locus_tag="PLQ18_13095"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNE02636.1"
                     /translation="MIFSGLPARIALLAFVPVLGLAALASAWLGYERYQAGVQEINTR
                     GTTLVRQAALALEGDPVLERERLRRYALGLLALDDVLTVSIDGLDGQTLVREQRSSSQ
                     RLNGNAGEQPEASLGFEAPVRGALAASLTPDISPGPGYLPGPARVHIELSRAPLFALR
                     SSVLIDTLGLLGLGALFGLLAASLGSIAILTPFERVQRLLNRLRNGQFEPPLRRGDDG
                     GPLDALEEGISAIARLLREQRQTLMSRDEQTMAELHETLEELEIKNAELDIARKKALA
                     ASQVKSEFLANMSHEIRTPMNGVLGFVELLAKTELSRTQQGHLQTIRASARHLMTILN
                     DILDFSKIEAGKFELAQQPFDLRELLENAVLLFAPNAHAKNLSLVLDVAPELPSGLIG
                     DGPRIAQIVTNLVSNAVKFTDRGQVEVCVRLCSETAKTVTFGIAVLDTGIGFAPQDRA
                     RLFSAFDQIDTSPTRRHGGTGLGLAICRRLVQMMGGDIHAESVPGEGACFEAT"
BASE COUNT          254 a          607 c          586 g          249 t
ORIGIN      
        1 gagggtcgga aacagggacg ggtcgatcat gggcggcagg tccggatggg gcaggggcgc
       61 cgcattcagg cgggccggct agtatacacg gcccgttccg gccgcccggc cgttgcccct
      121 gccgtccgat gctcctcctg ccgctgcctg ctgctgtcct gatccacgcc tgccggcgtg
      181 ccgagcgatg atcttctccg gtctgccggc gcgcatcgcg ctgcttgcct tcgtgccggt
      241 gctggggctg gccgcgctcg cttcggcctg gctcggctac gagcgctatc aggccggcgt
      301 gcaggagatc aacacccgcg ggacgacgct ggtgcgccag gcggccctgg ccctggaggg
      361 cgatccggtg ctcgaacgcg agcgcctgcg ccgctacgcg ctcgggctgc tcgccctcga
      421 tgacgtgctg accgtgagca tcgacggcct cgacggccag acgctggtgc gcgaacagcg
      481 cagcagcagc cagcgcctca acggcaacgc cggtgagcag cccgaagcct cgctcggctt
      541 cgaggcaccg gtgcgcgggg cgctggccgc cagcctgacg ccggacatca gccccgggcc
      601 gggttacctg ccggggccgg cgcgcgtgca catcgagctg tcgcgcgcgc cgctgttcgc
      661 gctgcgcagc agcgtgctga tcgacacgct cggcctgctc gggctcgggg cgctgttcgg
      721 gctgctggcc gcgagcctcg gttcgatcgc gatcctgacg ccgttcgaac gcgtgcagcg
      781 cctgctgaac cggctgcgca acggccagtt cgagccgccg ctgcgccgcg gcgacgacgg
      841 cgggccgctc gatgcgctgg aggaaggcat cagtgccatc gcccggctgc tgcgcgagca
      901 gcgccagacg ctgatgtcgc gcgacgagca gaccatggcc gaactgcacg agacgctgga
      961 ggaactcgag atcaagaacg ccgagctcga catcgcgcgc aagaaggcac tggccgcgag
     1021 ccaggtcaaa tccgaattcc tcgccaacat gagccacgag atccgcacgc cgatgaacgg
     1081 cgtgctcggc ttcgtcgagc tgctggccaa gaccgagctg tcccgcaccc agcaggggca
     1141 tctgcagacg atccgtgcct cggcacggca tctgatgacc atcctcaacg acatcctcga
     1201 cttctcgaag atcgaggccg gcaagttcga actcgcgcag cagcccttcg acctgcgcga
     1261 gctgctggag aacgccgtgc tgctgttcgc cccgaacgcg cacgccaaga acctgtcgct
     1321 ggtgctcgac gtggcgcccg agctgccctc ggggctgatc ggcgacgggc cgcgcatcgc
     1381 gcagatcgtc accaacctcg tcagcaacgc cgtcaagttc accgatcgcg gccaggtcga
     1441 ggtctgcgtg cggctgtgct ccgaaaccgc gaagaccgtg acgttcggca tcgccgtgct
     1501 cgataccggc atcggcttcg cgccgcagga ccgcgcacgc ctgttcagcg ccttcgacca
     1561 gatcgacacc tcgccgacgc gccgccacgg cggcaccggt ctcggactcg cgatctgccg
     1621 gcgcctggtg cagatgatgg gcggcgacat ccacgccgag agcgtgcccg gtgagggcgc
     1681 gtgcttcgag gcgacg
//
LOCUS       DAOQJH010000671         1696 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_63673_length_1696_cov_3.319927, whole genome shotgun sequence.
ACCESSION   DAOQJH010000671 DAOQJH010000000
VERSION     DAOQJH010000671.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1696)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1696)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1696
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63673_length_1696_cov_3.319927"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..978
                     /locus_tag="PLQ18_13100"
     CDS             <1..978
                     /locus_tag="PLQ18_13100"
                     /inference="COORDINATES: protein motif:HMM:NF023739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D C-terminal
                     domain-containing protein"
                     /protein_id="HNE02637.1"
                     /translation="AARLDAPSPLLDGLGIRVIGALPRTAARDFDAPRLVVPLYAADG
                     RVAARVAAVPFLRPADLPPCSEAEDPLIAGVEAVYAEALAAARAQRRPGEALLATGHA
                     YLVGGQLSALSERRILGGHQHALPVALFPEDVAYVALGHLHLAQRVAGRDAVRYAGAP
                     LPFAFAENRYPHQVLRVDLDGEQLAAVEPLPVPQHLELLRRPGDAAQGLDEVLEDLAQ
                     LALDPALPPARWPCLEVSVRLERPEPGLRRRIELALDQRPVRLLTVVAHHCGRGEALG
                     DEYGEAAGIEALAVGEVFRRCHVSRYRQEPDAELLEVFCKLLEEVEARK"
     gene            1147..>1696
                     /locus_tag="PLQ18_13105"
     CDS             1147..>1696
                     /locus_tag="PLQ18_13105"
                     /inference="COORDINATES: protein motif:HMM:NF024996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HNE02638.1"
                     /translation="MQKSIVALAVAGVLAASAVAQAETTLYGSARIGVEYVQGKGSGK
                     DSSWDVANQASRLGVKGSEDLGGGLSAIYQYEFGVNIDGNGTGANNPWGQRLSFVGLQ
                     GGFGTVALGRQYRPAYTFVSQYTDIFNGNITNPYSLTSNDQRMGNAVASVPPTFSGFT
                     AAAAVLMDGAANREDVDAFEAGI"
BASE COUNT          245 a          575 c          588 g          288 t
ORIGIN      
        1 gccgcacgcc tcgatgcgcc gtcaccgctg ctcgacggcc tcggcatccg ggtcatcggt
       61 gcgctgccgc gcacggccgc ccgcgacttc gacgccccgc gactggtcgt gccgctgtac
      121 gcggcagacg gccgcgtggc cgcgcgcgtc gccgccgtgc ccttcctgcg cccggccgac
      181 ctgccgcctt gctccgaggc cgaagacccg ctgatcgccg gcgtcgaggc cgtctacgcc
      241 gaagccctcg ccgccgcgcg cgcgcagcgc cggcccggcg aggcgctgct cgcgaccggg
      301 catgcgtatc tggtcggcgg acagctgtcg gcgctgtcgg agcggcgcat cctcggcggc
      361 caccagcacg cgctgccggt ggcgctgttt ccggaagatg tcgcttacgt cgcgctcggg
      421 catctgcatc tggcgcagcg ggtcgccggc cgtgacgcag tgcgctacgc cggagcgccg
      481 ctgccgttcg cgttcgccga gaaccgctat ccgcatcagg tgctgcgcgt cgatctcgac
      541 ggcgagcagc tggcggcggt ggagccgctg ccggtgccgc agcacctcga actgctgcgc
      601 cggccgggcg atgcagcgca ggggctcgac gaggtgctcg aagacctcgc gcaactcgcc
      661 ctcgacccgg cgctgccgcc tgcgcgctgg ccgtgtctgg aggtcagcgt gcgcctggag
      721 cgccccgagc cggggctgcg ccgtcgcatc gagctcgcgc tcgaccagcg gccggtgcgg
      781 ctgctgacgg tggtcgcgca tcactgcggt cgcggcgagg cgctcggcga tgagtacggc
      841 gaggccgccg gcatcgaggc cctggccgtc ggggaagttt tccggcgctg tcacgtcagc
      901 cggtatcggc aggagccgga cgcggagctg ctggaggtct tctgcaagtt gctggaagag
      961 gtggaggccc ggaaataaag gggcagatct gtgcggagca gatcaggtgg tgttgtagta
     1021 cagcaacgaa agagggcgtt tgttgtaact atgcttgtat gttgcggcgc gacacgctat
     1081 aaagtcgtca caaagttgcg ctagccgcgc gatcccgttt cccgacatcc gcacaggaga
     1141 tcgaacatgc agaagtccat cgttgcgctg gccgttgccg gcgttctggc cgcctcggcg
     1201 gttgcccagg ccgaaaccac gctgtacggt tcggcccgca tcggcgtcga atacgttcag
     1261 ggcaagggat ccggcaagga ttccagctgg gatgttgcca accaggcctc gcgcctcggt
     1321 gtcaagggtt cggaagacct gggtggcggt ctgagtgcga tctaccagta cgagttcggc
     1381 gtcaacatcg acggcaacgg taccggtgcc aacaatcctt ggggccagcg tctgagcttc
     1441 gtcggtctgc agggtggttt cggtaccgtc gcgctcggtc gccagtaccg tccggcctac
     1501 accttcgtca gccagtacac cgacatcttc aacggcaaca tcaccaaccc ctactcgctg
     1561 acctcgaatg accagcgcat gggtaatgcc gttgcctccg tgccgccgac cttcagcggc
     1621 ttcaccgccg ctgcggccgt cctgatggat ggtgctgcca accgtgaaga cgtcgatgcg
     1681 ttcgaagccg gtatcg
//
LOCUS       DAOQJH010000672         1695 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_63771_length_1695_cov_2.467683, whole genome shotgun sequence.
ACCESSION   DAOQJH010000672 DAOQJH010000000
VERSION     DAOQJH010000672.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1695)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1695)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1695
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63771_length_1695_cov_2.467683"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..661
                     /locus_tag="PLQ18_13110"
     CDS             <1..661
                     /locus_tag="PLQ18_13110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009668034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HNE02639.1"
                     /translation="ATPAATPAAARPAAPLPAPLAAPAGAMAQTTDVLVAIGTSTGGT
                     IALEQVLSTLPRVCPGIVIVQHMPELFTAAFARRLDSLCQLDVREAKTGDRVLAGTAL
                     IAPGGKHMVVRRSGAQYRVEVLDGPPVNRHRPSVDVLFRSVAECAGRNALGILMTGMG
                     ADGARGLLQMHEAGARTLAQDEASCVVYGMPKEAVKLGAVDRTLALDAIPQEIVGALR
                     H"
     gene            830..1627
                     /locus_tag="PLQ18_13115"
     CDS             830..1627
                     /locus_tag="PLQ18_13115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015486381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HNE02640.1"
                     /translation="MIDPGIRAEIVHRLEQAETEHGVRILFAIESGSRAWGFASPDSD
                     YDVRFIYMHAPQWYQAVDLEERRDVIEYPIVDEIDLNGWDIRKALRLYWKSNPAFVEW
                     IQSPITYLERGDFRSSAIELLPGVYAPVKGVHHYYSMARTNYRGYLREAVVPLKKYFY
                     VLRPLLAARWLMQTGSAAPIEFERLLCLLDDEPEVLAEVHGLLERKRNTPEIGRAAAI
                     PLLNAFIERELPDSPAPSLPATGKSSPSEVIDRLNALFHQTLREFHP"
BASE COUNT          285 a          607 c          530 g          273 t
ORIGIN      
        1 cgccaccccg gccgccactc cggccgccgc ccgtccggcc gcgccgctgc cggccccgct
       61 cgcggccccg gccggcgcga tggcgcagac caccgacgtg ctggtcgcga tcggcaccag
      121 caccggcggc acgatcgcgc tcgaacaggt gctctcgacg ctgccgcgcg tctgccccgg
      181 catcgtcatc gtccagcaca tgccggaact gttcaccgcc gccttcgcca gacgcctcga
      241 cagcctctgc cagctcgacg tgcgcgaggc gaagaccggt gatcgcgtgc tcgccggcac
      301 cgcgctgatc gcccccggcg gcaagcacat ggtcgtgcgc aggagcggcg cgcagtaccg
      361 cgtcgaggtg ctcgacggcc cgccggtcaa ccgccaccgg ccctcggtcg acgtgctgtt
      421 ccgctcggtc gccgaatgcg cgggccgcaa cgcgctcggc atcctgatga ccggcatggg
      481 cgccgacggc gcccgcggcc tgctgcagat gcacgaggcc ggcgcgcgca cgctggcgca
      541 ggacgaggcg agctgcgtcg tctacggcat gccgaaggag gccgtgaagc tcggcgccgt
      601 cgaccgcacg ctggcgctcg acgccatccc gcaggagatc gtcggggcgc tgcggcattg
      661 agcggatggt ccacgcctgc ccctctgccc ggcgaagccg cccccggacc cacgccgggc
      721 gttaccggta gcgactgacg accgcacgca gtgctccggg aaccgtcttg ccggtaccgg
      781 tcctgcgggg tagcgtgcat cgccccttcg ctgccggcca ggtttcacca tgatcgatcc
      841 cgggatcaga gctgaaatcg tccatcgcct cgaacaggcc gaaacagaac atggcgtgcg
      901 gattctgttt gccatcgaat ccggcagccg ggcctggggc tttgcctcac cggacagcga
      961 ttacgatgtc cgcttcatct atatgcacgc accgcagtgg tatcaggccg ttgatcttga
     1021 agagcgccgg gatgtcatcg aatacccgat cgtcgacgag atcgacctga acggctggga
     1081 tatcaggaag gccctgcggc tgtactggaa atcgaatccg gctttcgtcg aatggatcca
     1141 gtcgccgatc acctatctcg aacgcggcga tttccgcagc agcgccatcg agctgctgcc
     1201 gggcgtctac gcaccggtga aaggcgtcca tcactactac agcatggctc gcaccaatta
     1261 ccgcggctat ctgcgcgaag ccgtcgtgcc gctgaagaag tatttctatg tgctgcgccc
     1321 gctgctcgcg gcccgctggc tgatgcagac cggcagcgcc gcaccgatcg aattcgagcg
     1381 cctgctgtgc ctgctggacg acgagcccga agttctcgcc gaagtgcatg gcttgctgga
     1441 gcgcaagcgc aacactccgg aaatcggtcg cgccgcagcg attccactgc tgaacgcctt
     1501 catcgaacgc gagcttccgg actcccctgc gccgtcgctg ccagccaccg gaaaatcctc
     1561 cccgtccgaa gtcatcgacc ggctgaatgc cttgtttcac cagaccctgc gggagtttca
     1621 cccgtagcat gcagaacgcg gaaggcgggt tacggccaat gccattaagt tagccaaaaa
     1681 tctcagcctg atcag
//
LOCUS       DAOQJH010000673         1694 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_63801_length_1694_cov_4.306894, whole genome shotgun sequence.
ACCESSION   DAOQJH010000673 DAOQJH010000000
VERSION     DAOQJH010000673.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1694)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1694)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1694
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63801_length_1694_cov_4.306894"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..1129
                     /locus_tag="PLQ18_13120"
                     /pseudo
     CDS             1..1129
                     /locus_tag="PLQ18_13120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004538587.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
     gene            1250..>1694
                     /locus_tag="PLQ18_13125"
     CDS             1250..>1694
                     /locus_tag="PLQ18_13125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02641.1"
                     /translation="MPPALPCRCAYLALLLSSLQPATAMLLYDNGPESAAGGIRLDET
                     VIAQDFRLAAAALIDTVTFAGTERFVPSDFAWRIYADNSSKPGALLLEGSGTAVRTFL
                     GTTATTTRYRYTLDIGALMLAAGDYWLGLSGSIGTTWSGAPDNGSS"
BASE COUNT          253 a          622 c          569 g          250 t
ORIGIN      
        1 cggcggcgtg cgccgcggcg caccggatgc cgaccgcacg cgccggcacg gcgactggct
       61 gagccgcatg tatccgcggc tggcgctgat ccgcacgctg ctcgccgacg acggcctgct
      121 cgcgatccac atcgacgagc acgagcagcc ggcgctgacg ctgctgctgc gcgagctgtt
      181 cggcgaggcc aacgagctcg gcatcgcggt ctgggacaag cgcaacccca agggcgacgc
      241 gcgcggcatc gcctaccagc acgaatcgct gctgctgtac gcgcgcgacg ccgcgacgct
      301 gctggcaaga cagccgctgc gccggcccaa gcgccacgcg cagcagatgc tcgccgccgc
      361 ccgcaccgcc ctcgccgcga gtccgacgct cgaagaagcc cgcagccgct accgtgcctg
      421 gctgaagtcg cagccgcagc tctccggcgg cgaggcgatg tactggcggc tgtcggccga
      481 cgggcgcgtg taccggctgg tgtcgatggc ctggccgaac aagcaggcgc cgtcggccga
      541 gtacttcgag ccgctgatcc atccgcagac cggccgcccc tgcccggtgc cggtgcgcgg
      601 ctggcgcaac ccgcccgaga ccatgcgccg gctgctcgac accggccgga tcgagttcgg
      661 gccggatgag tcggtgcagc cgcaacgcat ctactacctc gacgagaacc tgtacgagaa
      721 cgtgccgtcg atcctgccct acggcggttc cgacgacgcc ttgctgcgca cgctcggcat
      781 cccgttcgac cagccgaagc cggtcgactt cgtcgcggcg gtgatcggct ggtgcacggg
      841 cggcgacgat ctcgtgctcg actgctgggc cggctccggc accaccgcgc acgcggtcat
      901 gaagctcaac gccggcgacg gcggccgcag acgcttcctg ctcgtgcagc gccccgagcc
      961 gctcgacccg cgcaagtccg gccagcgcgc agcgttcgcg ttctgcgagc agcacggact
     1021 ggctccggac ctcgccgggc tgtgccgcga gcgactgcgg cgcacggcgg cgctgctgcg
     1081 ggcggagttt ccggactggg acggggatgc ggggttccgg gtgtggcgct gcgccgagac
     1141 tccagcggac ggcaccgaat gcaccacctt gcccataccg tattgatttt catgaccaga
     1201 atgacaggag ctacgctccg ggtgccgttg ccaccggagt ctcgtcatca tgccgcccgc
     1261 cctgccctgc cgttgtgcat atctggctct gctgttgtcc tcgctgcagc ctgccactgc
     1321 catgctgctt tacgacaacg gtcccgaaag tgcagccggc ggcatccggc tggatgaaac
     1381 cgtcatcgcg caggatttcc ggctggcggc ggctgccctc atcgatacgg tcacgttcgc
     1441 cggcaccgaa cgcttcgtgc cgagcgactt cgcatggcgg atctatgccg acaacagcag
     1501 caagccgggg gcgctgctgc tggaaggcag cggtacggct gtccgcacct tcctcggcac
     1561 gacagcgacc accacccgct atcgctacac actggacatc ggcgccctga tgctcgctgc
     1621 cggcgactac tggctgggcc tgtccggcag tatcggcacg acctggtcgg gcgcaccgga
     1681 caacggcagc agcc
//
LOCUS       DAOQJH010000674         1694 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_63803_length_1694_cov_4.186089, whole genome shotgun sequence.
ACCESSION   DAOQJH010000674 DAOQJH010000000
VERSION     DAOQJH010000674.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1694)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1694)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1694
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63803_length_1694_cov_4.186089"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(97..453)
                     /locus_tag="PLQ18_13130"
     CDS             complement(97..453)
                     /locus_tag="PLQ18_13130"
                     /inference="COORDINATES: protein motif:HMM:NF016258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HNE02642.1"
                     /translation="MPRIPACLGLAALLVCGSGCSYFSDFSMPLPSFYKTKVRQGNYL
                     DEATLGTITPGMSKREVQQRLGTPLVTDPFHQNRWDYVYSYRPGTYDDGEPEQRRVTL
                     YFERDVLSRVEYPPQG"
     gene            540..974
                     /gene="fur"
                     /locus_tag="PLQ18_13135"
     CDS             540..974
                     /gene="fur"
                     /locus_tag="PLQ18_13135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018934735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HNE02643.1"
                     /translation="MGSKELKQVGLKVTLPRMKILAILQEHGGAQHLSAEDIYKSLIA
                     SGEEVGLATVYRVLTQFETAGLVKRHHFEGGQSVFELNQGEHHDHILCLQCGRIEEFV
                     DEVIETRQREIAAQHGFRLAEHSLILYGNCTNPDCPHRPPTH"
     gene            complement(1030..>1694)
                     /locus_tag="PLQ18_13140"
     CDS             complement(1030..>1694)
                     /locus_tag="PLQ18_13140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020483044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNE02644.1"
                     /translation="HYRDNDTGVHIWRMAAYAAALGAACGLDAVICRRLELAAPMHDT
                     GKLGIPDAILRKPGPLDADEWVIMRTHPRIGHDILARSQAPVFQMAAQIALRHHEKWD
                     GSGYPDGLCGTGIPETARIVAIADVFDALTMKRPYKEAWPAERALAELHKGAGRHFEP
                     RLVTAFADILPRILEIKAQWDEREAAGHATDLPTLPADYAPAPPGNAADAHAAGSDAR
                     PA"
BASE COUNT          257 a          559 c          615 g          263 t
ORIGIN      
        1 ggaagtccgc aagcagcgcg ctgccgaggg ccggctgaag aagcagcggc gtgaggacgc
       61 ggccggcgac tgaacgctcc cggccgcaag cccggctcag ccctgcggcg ggtattcgac
      121 gcggctcagc acgtcgcgct cgaagtacag cgtcacgcgc cgctgctcgg gctcgccgtc
      181 gtcgtaggtg ccgggacggt agctgtagac gtagtcccag cggttctggt ggaacggatc
      241 ggtgaccagc ggcgtgccga ggcgctgctg cacctcgcgc ttgctcatgc cgggcgtgat
      301 cgtgccgagt gtcgcctcgt cgaggtaatt cccctgccgg accttggtct tgtagaacga
      361 gggcagcggc atcgagaaat ccgagaaata cgagcagccg ctgccgcaga cgagcagtgc
      421 ggcgaggccg aggcaggcgg gaatccgggg catttgaggc ggcgggcacg gtgacttaga
      481 ctcgggcatc ataaccgatt ccgccggtcc ggctgacggc gcgcgaggag aaccggccct
      541 tgggctcgaa agaactgaag caggtgggcc tgaaggtcac gctgccgcgt atgaagatcc
      601 tcgcgatcct gcaggaacac ggcggcgcgc agcacctgag cgccgaagac atctacaagt
      661 ccctgatcgc gagcggcgag gaagtcggtc tggcgaccgt ctaccgcgtg ctgacgcagt
      721 tcgagaccgc cggcctggtc aagcgccatc acttcgaggg cggccagtcg gtgttcgagc
      781 tgaatcaggg cgagcaccac gatcacattc tgtgcctgca gtgcggccgc atcgaggagt
      841 tcgtcgatga ggtcatcgag acgcgccagc gcgagatcgc cgcgcagcac ggcttccggc
      901 tcgccgagca ctcgctgatc ctgtacggca actgcacgaa ccccgactgc ccgcaccggc
      961 cgccgaccca ctgaaacgcg cgcccgctcc ctcccgttcg ctctccgtcc cgaggctgct
     1021 gcggcctgct caggccgggc gtgcgtcgct gcctgcggcg tgcgcatcgg cagcgttacc
     1081 gggcggggcc ggcgcgtagt ccgcgggcag cgtcggcagg tcggtcgcgt gaccggcggc
     1141 ttcgcgctcg tcccactgcg ccttgatctc caggatgcgc ggcaggatgt cggcaaaggc
     1201 cgtcaccagc cgcggttcga agtggcggcc ggcgcccttg tgcagctccg ccagcgcccg
     1261 ttcggccggc caggcttcct tgtacgggcg cttcatcgtc agcgcatcga agacatcggc
     1321 gatcgcgacg atgcgcgcgg tctcggggat gccggtgccg cagaggccgt cggggtagcc
     1381 gctgccgtcc catttctcgt gatggcgcag cgcgatctgc gcggccatct ggaacaccgg
     1441 cgcctgactg cgcgcgagga tgtcgtggcc gatgcgcggg tgggtgcgca tgatcaccca
     1501 ttcgtccgcg tcgagcgggc cgggcttgcg caggatcgcg tccgggatgc cgagcttgcc
     1561 ggtgtcgtgc atcggtgcgg cgagttcgag ccgccggcag atcacggcat cgagcccgca
     1621 ggcggcgccg agggccgcgg cgtaggcggc catgcgccag atgtgcacgc cggtgtcatt
     1681 gtcgcggtaa tggc
//
LOCUS       DAOQJH010000675         1694 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_63805_length_1694_cov_4.075656, whole genome shotgun sequence.
ACCESSION   DAOQJH010000675 DAOQJH010000000
VERSION     DAOQJH010000675.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1694)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1694)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1694
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63805_length_1694_cov_4.075656"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..279
                     /locus_tag="PLQ18_13145"
     CDS             <1..279
                     /locus_tag="PLQ18_13145"
                     /inference="COORDINATES: protein motif:HMM:NF017496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HNE02645.1"
                     /translation="GLPLHAPSLLIEVATAATAGADRGEGFEQARTLPTLREYVIVEA
                     GRARVDVYRRRADGGWLLDSHGAGEGFVLAAIDGTIEVDALYEGARLR"
     gene            complement(299..937)
                     /locus_tag="PLQ18_13150"
     CDS             complement(299..937)
                     /locus_tag="PLQ18_13150"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014030063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HNE02646.1"
                     /translation="MSDIEKLIRGFQRFRARYFERDRELFQQLAREGQTPRTLVIACS
                     DSRVDPAIITDAEPGDLFVIRNVANLVPPAEDSGAFHGTSAAVQFAVCDLEVRHIVVM
                     GHGHCGGIRALLEGDAETGYIGNWVKIAGRARAKVLERWPDASPAFRQRAAERMSIIV
                     SLDNLMTFDFVRTRVEAGTLKLFGWYFDIENGEMMQYDPEARRFHDLYLGWD"
     gene            1007..1297
                     /locus_tag="PLQ18_13155"
     CDS             1007..1297
                     /locus_tag="PLQ18_13155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018869215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HNE02647.1"
                     /translation="MHCSIYRSRRKTDAYLYCAAKDDFSAVPKALLDQLGQLEHAMDL
                     ELTPERRLAREDAATVLDALATRGWYLQMPQREDWAADWRAAQTPAWGQRVD"
     gene            1384..>1694
                     /locus_tag="PLQ18_13160"
     CDS             1384..>1694
                     /locus_tag="PLQ18_13160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019365563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HNE02648.1"
                     /translation="MQFSERLDRLTSSLIRELLALTQQPDMISFAGGLPAAGAMPPLA
                     LANLPPALRQYGTTDGEPELRAAIARQLTGLGRACRPDQVLVTSGSQQGLDLVAKLFI
                     DP"
BASE COUNT          258 a          600 c          592 g          244 t
ORIGIN      
        1 ggcctgccgc tgcacgcgcc gtcgctgctg atcgaggtcg ccaccgccgc cacggccggg
       61 gccgaccgtg gcgagggctt cgagcaggcg cgcacgctgc cgacgctgcg cgagtacgtg
      121 atcgtcgagg ccggccgggc gcgcgtcgat gtctaccgcc gtcgcgccga cggcggctgg
      181 ctgctcgaca gccacggcgc cggcgaaggc ttcgtgctgg cagcgatcga cggcacgatc
      241 gaggtcgatg cgctgtacga gggcgcgcgg ctgcgctgaa ggagcggccc gggcgggttc
      301 agtcccagcc gagatacagg tcgtggaaac ggcgggcctc ggggtcgtac tgcatcatct
      361 cgccgttctc gatatcgaaa taccagccga acagcttcag cgtgccggcc tcgacgcgcg
      421 tgcgcacgaa gtcgaaggtc atcaggttgt cgagcgagac gatgatcgac atgcgctcgg
      481 cggcacgctg gcggaaggcc ggcgaggcat ccggccagcg ttcgagcacc ttggcgcgcg
      541 cgcggccggc gatcttcacc cagttgccga tgtagccggt ctcggcgtcg ccttcgagca
      601 gcgcgcggat gccgccgcag tggccgtggc ccatgaccac gatgtggcgc acttccaggt
      661 cgcagaccgc gaactgcacc gcggcgctgg tgccatggaa ggcgccggaa tcctcggccg
      721 gcggcacgag gttggcgacg ttgcggatca cgaacaggtc gccgggctcg gcatcggtga
      781 tgatcgccgg gtcgacgcgc gaatccgaac aggcaatgac cagcgtgcgc ggcgtctggc
      841 cttcgcgcgc cagctgctgg aacagctcgc ggtcgcgctc gaagtagcgc gcgcgaaagc
      901 gctggaagcc ccggatcagc ttttcgatgt cggacatgga cggactccgc ggttcggact
      961 tcgggcatca tatcgcccct ttcccggcca ccaagcgacc gccgccatgc actgctcgat
     1021 ctatcgcagc cgccgcaaga ccgacgccta cctgtactgc gccgcgaagg atgacttctc
     1081 ggccgtgccg aaggcgctgc tcgaccagct cggccagctc gagcacgcga tggatctgga
     1141 gctgacgccc gagcgcaggc tcgcgcgcga ggacgcggcc accgtgctcg atgcgctcgc
     1201 cacgcgcggc tggtacctgc agatgccgca gcgcgaggac tgggccgccg actggcgtgc
     1261 ggcgcagacg ccggcctggg gccagcgcgt cgactgaaga ccggcccgcc gcggcaagga
     1321 cttgcacgca ccgtacggaa agcgttagct agaacgcttg tcccgcccgc cgccggaatc
     1381 gtcatgcagt tctccgaacg cctcgaccgc ctcaccagtt cgctgatccg cgaactgctc
     1441 gcgctgaccc agcagcccga catgatctcg ttcgccggcg gcctgccggc ggccggcgcg
     1501 atgccgccgc tcgcgctcgc gaacctgccg ccggcgctgc gccagtacgg cacgaccgac
     1561 ggcgaacctg agctgcgcgc ggcgatcgcc cgccagctca ccggactcgg tcgcgcctgc
     1621 cggcccgatc aggtgctggt caccagcggt tcgcagcagg gcctcgatct ggtcgcgaag
     1681 ctgttcatcg atcc
//
LOCUS       DAOQJH010000676         1684 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_64635_length_1684_cov_2.481277, whole genome shotgun sequence.
ACCESSION   DAOQJH010000676 DAOQJH010000000
VERSION     DAOQJH010000676.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1684)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1684)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1684
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64635_length_1684_cov_2.481277"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..633
                     /locus_tag="PLQ18_13165"
     CDS             <1..633
                     /locus_tag="PLQ18_13165"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNE02649.1"
                     /translation="CRGVPLIAVAGNHEYYAGPADTGAVQARLQALSDEGVLHWLERR
                     SLLLDGLRILGTTLWTDFALYGDAGAGMAACAVVMQDFRGAIRQAGRRLEPADTLAWH
                     RRDRDWLAAELARPFPGPTVVLTHHAPHPDCIEPFYAGDPVSTGFASDLSPLFDGRIA
                     LWVHGHCHRATDFRIGGTRIVSNPHGYPDEPAAQTGFEPGRIIDLPTAAG"
     gene            complement(667..>1684)
                     /locus_tag="PLQ18_13170"
     CDS             complement(667..>1684)
                     /locus_tag="PLQ18_13170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008495370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNE02650.1"
                     /translation="RKPHSRASGRVTLADVAARAGVSAISVSRTLQSPAQVSAALRAR
                     IQAAIAELGYVPDHAARSLASARSGTVVVLVPSLGNAVFVETLTAIQAVLQPRGWQML
                     IGNTRYSPAEEEALLLAWLGHRPDGILLSGLDQTGRTRALLAGSGAPVVHMMEIDATG
                     ACCSVGSGQEAAGAAMAGHLLARGYRRIAFIAVQLDPRTRQRREGWRQVMRDAGCYEP
                     ARDLESPQPSSVGLGVEMLERLLAQAPDSDAVFCCNDDLAHGVLFECQRRGIDVPGRL
                     AVAGFNDLPASAWTHPRLTTIAIPRTGIGQEAARLLTELMAGRTPAQRTIDLGFELKA
                     RDST"
BASE COUNT          216 a          611 c          635 g          222 t
ORIGIN      
        1 tgcaggggcg tgccgctgat cgcggtcgcc ggcaaccacg agtactacgc gggcccggcc
       61 gacaccggcg cggtgcaggc ccggctgcag gcgctgagcg acgagggcgt gctgcactgg
      121 ctggagcggc gcagcctgct gctcgacggc ctgcgcatcc ttggcacgac gctctggacc
      181 gacttcgcgc tgtacggtga tgccggggcc ggcatggcgg cctgcgcggt ggtcatgcag
      241 gatttccgcg gcgcgatccg tcaggccggc cgccggctgg agccggccga cacgctggcc
      301 tggcaccggc gcgaccgcga ctggctcgcc gccgaactcg cccggccgtt tcccggcccg
      361 accgtcgtgc tcacgcacca cgcgccgcac ccggactgca tcgagccctt ctacgccggc
      421 gatccggtat cgacgggctt tgcgtcggac ctgagtccgc tgttcgacgg gcgcatcgcg
      481 ctgtgggtgc acgggcactg ccaccgggcg acggacttcc gcatcggtgg cacgcgcatc
      541 gtctcgaatc cgcacggcta tccggacgag ccggccgcgc agacgggctt cgagccgggc
      601 cggatcatcg acctgccgac cgcggccggc tgaggcagcg cgtgccccgg cagcgggcgc
      661 ggcgcttcag gtgctgtcgc gggccttcag ctcgaagccg agatcgatcg tccgctgcgc
      721 cggcgtgcgc ccggccatca gttcggtcag cagccgcgcc gcttcctggc cgatcccggt
      781 gcgcgggatg gcgatcgtgg tcaggcgcgg atgcgtccac gccgaggccg gcaggtcgtt
      841 gaagccggcc acggccagcc ggccgggcac gtcgatgccg cggcgctggc attcgaacag
      901 cacgccgtgc gcgagatcgt cgttgcagca gaacaccgca tcgctgtccg gcgcctgcgc
      961 gagcagccgc tccagcattt ccacgccgag gccgacgctg gagggctgcg gcgattcgag
     1021 gtcgcgcgcc ggctcgtagc agccggcatc ccgcatgacc tgccgccagc cctcgcgccg
     1081 ctgccgggtg cgcgggtcga gctgcacggc gatgaaggcg atgcgccggt agccgcgcgc
     1141 cagcagatgc ccggccatcg ctgcgccggc ggcctcctgc ccgctgccga cgctgcagca
     1201 ggcgccggtg gcatcgattt ccatcatgtg cacgacgggc gcgccgctgc cggccagcag
     1261 cgcccgggtc cgtccggtct gatcgagccc gctgagcagg atgccgtcgg gccggtgacc
     1321 gagccaggcg agcagcaggg cctcctcctc ggccggtgaa tagcgcgtgt tgccgatcag
     1381 catctgccag ccgcggggct gcagcacggc ctggatggcg gtcagcgtct cgacgaacac
     1441 cgcgttgccg agcgagggca cgagcacgac gacggtgccg gagcgcgccg aggccagcga
     1501 gcgggcggcg tgatccggca cgtagccgag ttcggcgatc gccgcctgga tgcgggcacg
     1561 cagcgccgcc gacacctgcg ccggggactg cagcgtgcgc gagaccgaaa tggcgctgac
     1621 cccggcgcgg gcggcgacat cggcgagggt gacgcggccg ctggcgcggc tatggggctt
     1681 gcgg
//
LOCUS       DAOQJH010000677         1682 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_64752_length_1682_cov_3.319607, whole genome shotgun sequence.
ACCESSION   DAOQJH010000677 DAOQJH010000000
VERSION     DAOQJH010000677.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1682
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64752_length_1682_cov_3.319607"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(284..517)
                     /locus_tag="PLQ18_13175"
     CDS             complement(284..517)
                     /locus_tag="PLQ18_13175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02651.1"
                     /translation="MTYVYQCHPSRLPQGPYGQMLEELTEARRLGLPASERVAIITRY
                     RALWGCMEDGPVHCQRGPDCIDCFLRHLLSQDY"
     gene            complement(781..1029)
                     /locus_tag="PLQ18_13180"
     CDS             complement(781..1029)
                     /locus_tag="PLQ18_13180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02652.1"
                     /translation="MRRLTGSLMLLAAHTAFAGGAYLKQDITGRWHYPELHPVQISAE
                     ETAGDSRGDEHAHANREPTRTVIAPPAAQPPAPATKPR"
     gene            1302..>1682
                     /locus_tag="PLQ18_13185"
     CDS             1302..>1682
                     /locus_tag="PLQ18_13185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012592582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase component 1"
                     /protein_id="HNE02653.1"
                     /translation="MSTLTQKVQDVFNEPGCDHNQSKSAKERKKGCTKQLTPGAAAGG
                     CAFDGAKIALQPITDVAHIVHGPIACEGNSWDNRHAASSGPTLYRTGFTTDINELDVI
                     YGGEKRLFKAIREAIEKYDPPAVFV"
BASE COUNT          274 a          520 c          605 g          283 t
ORIGIN      
        1 cgccgcttcg aggcgctggc gctcgcgctg atggcgaccg atcgcggcga tggcctcgat
       61 ggcttcctcg cgcgtctgcg gtaccggcca ctgccggcgg gcttcgaggc gcacgccgtt
      121 gcggcgggcg gtgacggggg gtttcgggtg tcccatgacg gctgacctcg cggggctgcg
      181 gcaactgccg gggatgcagg ggcaccgggt cgccggtgcc tgcatgcctg gggttgcgag
      241 ccgtggcgcg gcggaatgtt ccgcgcacgc gggcgggggg aggtcagtag tcctggctca
      301 gcaggtggcg caggaagcag tcgatgcagt cggggccgcg ctggcagtgc accggcccgt
      361 cctccatgca gccccacagt gcgcggtagc gcgtgatgat cgcgacgcgc tcgctggccg
      421 gcagaccgag ccggcgcgct tcggtcagct cttcgagcat ctggccgtag gggccttgcg
      481 gcaggcgcga gggatgacac tggtaaacgt aagtcatgga ctgtcaaggc cgactcggtc
      541 gaaggacagg ctgccgatgc tgacggcccc gacggttgcc gacgatacgg cagatgcagt
      601 aggcgcagga ccctgcgtat tgccggcttg acagggacgg cgctcattcc cgtccggccg
      661 cgccggggcg tcaatcagga ttgacgtaca cggcgtgtcg ccatcgtcag aagcggtttc
      721 ttcgtgcgcc gctgagaaaa tttcaggtga tccgccaagc agggcacgcg cacggcactg
      781 tcagcgcggt ttcgtcgcgg gcgccggtgg ctgggcggcc ggaggcgcga tgacggtgcg
      841 ggtcggttcg cggttcgcgt gggcgtgctc atcgccgcgg gaatcgcctg cggtttcctc
      901 ggcactgatc tgcaccggat gcagttccgg gtaatgccag cgcccggtga tgtcctgctt
      961 cagataggca ccgcctgcga acgcggtgtg tgctgccagc agcatcagac tccccgtcag
     1021 tctgcgcatg tgggtttcct cgtctggacc gtaccggcct atcggcagcc ggagccgcga
     1081 ctgtagccgg attcgccatg gtcgtcgtcg cgacgtagga attgcgacaa aacctacgcg
     1141 gccaccggga gatcgtccgg agtccgtcca ttggcccggc aagggcaaat ctggttcaag
     1201 tggctgtatc ggctgatgaa tccggcagga tcacgggctg gcatcgatcc tgctctgtcc
     1261 acgcaaacgg taacccgccg ttgcgaacgg gacagacgcc gatgagcacg ctgacgcaga
     1321 aggtccagga tgtcttcaat gagcccggct gcgaccacaa tcagagcaag agtgcgaagg
     1381 agcgcaagaa gggctgcacc aagcagctga cgcccggtgc cgcggcgggt ggctgtgcct
     1441 tcgacggcgc gaagatcgcg ctgcagccga tcaccgacgt tgcgcacatc gtgcatggcc
     1501 cgatcgcctg cgagggtaat tcctgggaca accgccatgc ggcgtcctcg ggtccgacgc
     1561 tgtatcgcac cggcttcacg accgacatca acgaactcga cgtgatctac ggcggcgaga
     1621 agcggctgtt caaggcgatc cgcgaagcga tcgaaaaata cgacccgccg gcggtgttcg
     1681 tg
//
LOCUS       DAOQJH010000678         1676 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_65231_length_1676_cov_7.692165, whole genome shotgun sequence.
ACCESSION   DAOQJH010000678 DAOQJH010000000
VERSION     DAOQJH010000678.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1676
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65231_length_1676_cov_7.692165"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..796)
                     /locus_tag="PLQ18_13190"
     CDS             complement(<1..796)
                     /locus_tag="PLQ18_13190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNE02654.1"
                     /translation="MRFVFRLLATLPLPLLHVLGALAGWLVYLASPTYRRHMRENMAL
                     ALGPAAAAARGAAVTAAGRMSLELPKIWLNPLEQAVGMMREVIGWEVVEAAQAAGKGI
                     VFLTPHLGCFELTAQYYSARAPITVLYRPPRYTVLQEMIEAGRARPMLHLAAADLSGV
                     RGLIKALKRGEAVGLLPDQAPKMGEGKWLPFFGKPAYTMTLAARLTETGAAVIFAFGE
                     RLPGGAGYRMHLRGPQFPIEGTLEERATAINREIEALIRSCPGQYLW"
     gene            1018..>1676
                     /gene="metK"
                     /locus_tag="PLQ18_13195"
     CDS             1018..>1676
                     /gene="metK"
                     /locus_tag="PLQ18_13195"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004315758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HNE02655.1"
                     /translation="MAEYLFSSESVGEGHPDKVADQISDAVLDAILAVDPTGRVACET
                     LVSTGLVVISGEITTKAHVNYREIAQETVRRIGYTDSDIGFDYKSCAILTAINTQSPD
                     IAQGVNEGQGLDLDQGAGDQGLMFGYACRETETLMPFPIYYAHRIMQRQAEVRRDGRL
                     PWLRPDAKSQLTVKYVDGKPVSIDTVVVSTQHSPDVSHAQLSEAVIEEIIKPVLPKDM
                     LT"
BASE COUNT          325 a          565 c          520 g          266 t
ORIGIN      
        1 cccacagata ctgcccgggg caactgcgga tcagcgcctc gatctcccgg ttgatggccg
       61 tggcacgttc ctccagggta ccttcgatcg ggaactgcgg cccgcgcagg tgcatgcgat
      121 agccggcgcc cccgggcaga cgctccccga aggcgaagat gacggccgcc cccgtttcgg
      181 tgagccgtgc ggccaacgtc atcgtgtagg caggcttgcc gaagaaaggc agccacttgc
      241 cctcacccat tttcggtgcc tgatcaggca gtagtccaac ggcttcgccc cgtttcagcg
      301 ccttgatcag tccgcgaacg ccggagagat ccgctgccgc aaggtgcaac atcggacgcg
      361 cccggccggc ttcgatcatt tcctgcagca ccgtgtagcg cggcggccgg tagaggacgg
      421 tgatcggcgc ccgcgccgag tagtactgcg ccgtcagttc gaagcacccg aggtgcgggg
      481 tcaggaagac gatgcccttg ccagccgcct gcgcggcctc gacgacttcc cagccgatca
      541 cctcgcgcat catgccgacg gcctgctcaa gcgggttcag ccagattttc ggcagctcca
      601 ggctcatgcg tccggcggca gtcactgcgg caccccgcgc ggcagcggct gccgggccga
      661 gcgccagcgc catgttctca cgcatgtgcc ggcgataggt cggcgatgcc agatagacca
      721 gccagccggc gagcgccccc agcacatgca gcaggggcag cggcagcgta gcaagaaggc
      781 ggaaaacgaa acgcatgggg cggcgggcag ggagcggatt tgccgaaagg gcgaaaaggg
      841 ctacaattat cgttccgccg agttaatagg acaacttgca gggcggggag gggtaaatcc
      901 tccgggagcg aacaaaacga tatcgcgcct gccaaatact gctaaagcgt tcgtctgctc
      961 gagaccccgg agccggcaac gccggaaaaa ccggggtttt ttgtttcagg agcagtcatg
     1021 gctgaatatc tcttttcttc cgaatccgtc ggcgaaggcc atcccgacaa ggttgccgac
     1081 cagatttccg atgccgtcct cgacgcgatc ctcgctgtcg acccgaccgg ccgcgtcgcc
     1141 tgcgaaaccc tggtctccac cggcctcgtc gtgatttccg gcgaaatcac caccaaggcc
     1201 cacgtcaatt accgcgagat cgcgcaggaa accgtgcgtc gcatcggcta caccgacagc
     1261 gacatcggct tcgactacaa gtcctgcgcc atcctcaccg ccatcaacac ccagtcgccg
     1321 gacatcgcgc agggtgtgaa cgaaggccag ggcctggacc tcgaccaggg cgccggcgat
     1381 cagggcctga tgttcggcta cgcctgccgc gaaaccgaaa cgctgatgcc cttcccgatc
     1441 tactacgcgc accgcatcat gcagcgccag gctgaagtgc gccgtgacgg ccgcctgccc
     1501 tggctgcgcc cggatgccaa gtcgcagctg acggtgaagt acgttgacgg caagccggtc
     1561 tcgatcgaca ccgtcgtcgt gtcgacccag cacagcccgg acgtgtcgca cgcccagctg
     1621 tcggaagccg tgatcgagga aatcatcaag ccggtcctgc cgaaggacat gctgac
//
LOCUS       DAOQJH010000679         1669 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_65788_length_1669_cov_5.348823, whole genome shotgun sequence.
ACCESSION   DAOQJH010000679 DAOQJH010000000
VERSION     DAOQJH010000679.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1669)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1669)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1669
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65788_length_1669_cov_5.348823"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..777)
                     /locus_tag="PLQ18_13200"
     CDS             complement(<1..777)
                     /locus_tag="PLQ18_13200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014995017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNE02656.1"
                     /translation="MAKTYDVVVIGGGPAGYVAAIRCAQLGLATACVEKWINIEGKPA
                     LGGTCLNVGCIPSKALLESSEHYHKLQHGLEAHGISVGEASVDVAKMIGRKEQIVSQL
                     TGGIAQLFKANKVDWLQGRGKLLAERQVEVTGHDGAVEVVEADNVIIAAGSVPIDIGA
                     APKDDKLIVDSTGALAFTEVPKRFGVIGAGVIGLELGSVWKRLGAEVVILEAVEDFLA
                     AADHQIAKDALRQFQKQGLDIRLGARVTGSTKTDTEVTVQY"
     gene            complement(889..>1669)
                     /gene="odhB"
                     /locus_tag="PLQ18_13205"
     CDS             complement(889..>1669)
                     /gene="odhB"
                     /locus_tag="PLQ18_13205"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008349242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HNE02657.1"
                     /translation="KPAAPAPAAKPAATPAALPAGAWPFSKRMEQRVPMTRLRARIAE
                     RLLEAKNTTAMLTTFNEINMKPVIDLRNKYKDQFEKSHGVKLGFMSFFVKAVVESLKR
                     YPAVNASIDGNDIVYHDYYDVGIAVSSPRGLVVPILRNAEDMSLADIEKAIGDFGKRA
                     KEGSLTVEEMTGGTFTITNGGVFGSLMSTPILNPPQSGILGMHATKDRAVVENGQVVA
                     RPMMYVAHSYDHRIIDGKEAVGFLVAVKEAIEDPARMLLSV"
BASE COUNT          251 a          541 c          580 g          297 t
ORIGIN      
        1 gtactggacc gtgacctcgg tgtcggtctt ggtcgagccg gtcacgcgcg caccgaggcg
       61 gatgtcgagg ccctgcttct ggaactggcg cagcgcatcc ttggcgatct gatggtcggc
      121 ggcggcgagg aagtcctcga ccgcttcgag gatcacgacc tcggcgccga ggcgcttcca
      181 gaccgagccg agttcgaggc cgatgacgcc ggcaccgatg acgccgaagc gcttcggcac
      241 ctcggtgaag gcgagcgcgc cggtcgaatc gacgatcagc ttgtcatcct tcggcgccgc
      301 gccgatgtcg atcggcaccg agccggcggc gatgatgacg ttgtcggcct cgaccacctc
      361 gaccgcgccg tcgtggccgg tgacctcgac ctggcgctcg gcgagcagct tgccgcggcc
      421 ctgcagccag tcgaccttgt tggccttgaa cagctgcgcg atgccgccgg tcagctgcga
      481 gacgatctgc tccttgcggc cgatcatctt cgcgacatcg accgacgcct cgccgacgct
      541 gatgccgtgg gcttcgaggc cgtgctgcag cttgtggtag tgctcggagg attcgagcag
      601 tgccttggac gggatgcagc cgacgttcag gcaggtgccg ccgagtgcgg gcttgccttc
      661 gatgttgatc cacttctcga cgcaggcggt cgcgaggccg agctgcgcgc agcggattgc
      721 cgcgacgtag ccggccgggc cgccgccgat gacgacgacg tcataagtct tggccatgac
      781 agtctcccta aaagagtgct tccggtaagg gatggggcgc aaacgaacgg gcaggcgcga
      841 cggggcaggg gtgccggacc cccgccccga aatcgccggg tcgtgcgctc agacggagag
      901 cagcatccgc gccggatcct cgatcgcttc cttgacggcg acgaggaagc cgaccgcctc
      961 cttgccgtcg atgatgcggt gatcgtagga gtgcgcgacg tacatcatcg gccgtgcgac
     1021 gacctggccg ttctcgacga cggcgcggtc cttggtcgcg tgcatgccga ggatgccgct
     1081 ctgcgggggg ttcaggatcg gcgtcgacat cagcgagccg aacacgccgc cgttggtgat
     1141 cgtgaaggtg ccgccggtca tttcctcgac ggtcagcgag ccttccttcg cgcgcttgcc
     1201 gaagtcaccg atcgccttct cgatgtcggc gagcgacatg tcctcggcgt tgcgcaggat
     1261 cggcacgacc agaccgcgcg gcgagctgac cgcgatgccg acgtcgtagt agtcgtggta
     1321 gacgatgtcg ttgccgtcga tcgaggcatt gaccgccggg tagcgcttca gcgactcgac
     1381 caccgccttg acgaagaacg acatgaaacc gagcttgacg ccgtgcgact tctcgaactg
     1441 gtccttgtac ttgttgcgca ggtcgatgac cggcttcatg ttgatttcgt tgaacgtcgt
     1501 cagcatcgcc gtggtgttct tcgcctccag caggcgttcg gcgatgcggg cgcgcagacg
     1561 cgtcatcggc acgcgctgtt ccatgcgctt cgagaacggc caggcaccgg ccggcagcgc
     1621 ggccggcgtg gccgccggct tcgcggccgg cgccggggcg gccggcttc
//
LOCUS       DAOQJH010000680         1666 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_66019_length_1666_cov_2.908132, whole genome shotgun sequence.
ACCESSION   DAOQJH010000680 DAOQJH010000000
VERSION     DAOQJH010000680.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1666)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1666)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1666
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66019_length_1666_cov_2.908132"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1175
                     /locus_tag="PLQ18_13210"
     CDS             <1..1175
                     /locus_tag="PLQ18_13210"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE02658.1"
                     /translation="DEYAAAADAAGIRIDIDAAGELSVNADPERLTQVLGHLLDNALR
                     HAPAGSAVTLRALKLPGRRAGIEVCDRGAGVPAELRPRLFHRFLQGPVETGRPAGGAG
                     LGLYIARELTERQGGTINYETGPEGTCLHLELPLAVVTSTHSERPHVLVVEDDALAAS
                     AFVAELSRDGYIAEVAGSVRAAREKLAVGRYALVTVDMLLPDGMGEEVIRAMREDPRH
                     VAVPVMVVSALPQGSLEAVALPVADWLPKPVRPERLQAAIAALGVHAERAQVLHVEND
                     PGMREYVRRLLGPRVKLFDAETLSEAEHLAATLPFDLAILDLLLPDGYGLAVAPKLHT
                     AGGEPTPVLVFSAGEPDPAQWQAIAGTLSKASATGKDLLRTVGSLLGRDREGPPHE"
     gene            1168..>1666
                     /locus_tag="PLQ18_13215"
     CDS             1168..>1666
                     /locus_tag="PLQ18_13215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02659.1"
                     /translation="MNDPAAAGTLRLLYIDDDPEQRLLVRLALERLGGWQITLAGSAD
                     AQLPALAQRTGAGCVLLATAPDPAATEAVLALLRADERSAALPVVILAVLAPPAAAAR
                     WRSLGALEAITGPLVPSALPARLRALRTVRRTEPGAAAPAAPEADADPIADLRRSFLA
                     QLPERR"
BASE COUNT          213 a          652 c          601 g          200 t
ORIGIN      
        1 gcgacgaata cgccgcggcc gcggacgcgg ccgggatcag gatcgacatc gacgccgccg
       61 gcgaactcag cgtcaacgcc gaccccgagc ggctgacgca ggtgctcggc catctgctcg
      121 acaacgcgct gcgccatgcc ccggccggca gcgcagtgac gctgcgcgcg ctgaaactgc
      181 cgggccggcg cgccggcatc gaagtctgcg accgcggtgc cggcgtcccg gccgagctgc
      241 gcccgcggct gttccaccgc ttcctgcagg gaccggtcga gaccggccgg cccgccggcg
      301 gcgccggtct cggcctgtac atcgcccgcg agctgaccga gcgccagggc ggcacgatca
      361 actacgagac cggccccgag ggcacctgcc tgcacctcga actgccgctg gccgtggtca
      421 cgagcacgca cagcgagcgc ccgcacgtgc tcgtggtcga ggatgacgcg ctcgcggcct
      481 cggccttcgt cgccgagctg agccgcgacg gctacatcgc cgaggtcgcc ggcagcgtgc
      541 gcgccgcgcg cgagaaactc gccgtcggcc gctacgcgct ggtcaccgtc gacatgctgc
      601 tgcccgacgg catgggcgag gaagtcatcc gcgcgatgcg cgaggacccg cgccacgtcg
      661 ccgtgccggt catggtcgtc tcggcgctgc cgcagggctc gctggaagcc gtggcgctgc
      721 cggtcgccga ctggctgccg aagccggtgc ggcccgagcg cctgcaggca gcgatcgccg
      781 cgctcggcgt gcacgccgag cgcgcgcagg tgctgcacgt cgagaacgat cccggcatgc
      841 gcgaatacgt gcgccgcctg ctcggcccgc gcgtgaagct cttcgatgcc gaaaccctgt
      901 ccgaagccga gcacctggcc gcgacgctgc cgttcgatct ggcgatcctc gacctgctgc
      961 tgcccgatgg ctacggcctc gccgtggcgc cgaagctgca caccgccggc ggcgagccga
     1021 cgccggtgct ggtgttctcg gccggcgaac ccgaccccgc ccagtggcag gcgatcgccg
     1081 gcaccctgag caaggcgagc gccaccggca aggacctgct gcgcacggtc ggctcgctgc
     1141 tcgggcgcga ccgcgaggga ccgccccatg aatgatcctg ccgccgccgg cacgctgcgc
     1201 ctgctgtaca tcgacgacga ccccgaacag cgcctgctcg tgcgcctcgc gctcgaacgg
     1261 ctcggcggct ggcagatcac gctggccggc agcgccgatg cgcaactgcc ggcgctcgcg
     1321 cagcgcaccg gcgccggctg cgtgctgctc gcgacggcac ccgacccggc agcgaccgaa
     1381 gccgtgctgg ccctgctgcg ggccgatgag cgcagcgcgg cgctgccggt ggtgatcctc
     1441 gccgtgctgg caccgccggc ggccgccgcg cgctggcgct cgctcggtgc gctggaggcg
     1501 atcaccggac cgctggtgcc gagcgcgctg ccggctaggc tgcgggcgct gcgcacggtc
     1561 cggcgcaccg agcccggcgc cgcggcgccg gccgcgcccg aagccgatgc cgacccgatc
     1621 gccgacctgc gccggagctt tcttgcgcag ctgcccgaac ggcgcc
//
LOCUS       DAOQJH010000681         1666 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_66043_length_1666_cov_2.505897, whole genome shotgun sequence.
ACCESSION   DAOQJH010000681 DAOQJH010000000
VERSION     DAOQJH010000681.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1666)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1666)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1666
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66043_length_1666_cov_2.505897"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..397)
                     /locus_tag="PLQ18_13220"
     CDS             complement(<1..397)
                     /locus_tag="PLQ18_13220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011811863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HNE02660.1"
                     /translation="MRGRIIFLLEEPSMATLLNGLLPRMFPGWAAGQQFLCVPHEGKS
                     DLERSLPRKLRNWQIPDDRFVVVRDNDAHPDCHVHKRRLSESCASHGRPDTLVRLICQ
                     ELESWYVGDLAALALAYEMPSLDSTRSRRK"
     gene            complement(398..1585)
                     /locus_tag="PLQ18_13225"
     CDS             complement(398..1585)
                     /locus_tag="PLQ18_13225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010381256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNE02661.1"
                     /translation="MQIEEIEIRNYRVFRHVRIRSVPRLCVLVGANGTGKSTLFDVFS
                     FLKDALSMNVGKAIARRGGYRELASREHEEDPIELTLRFRMEIAGHSRMITYQLQIGL
                     DASLRKPVVMREVLRYKRGSHGRPFHFLDFSNGSGYAITNEEDFNRPDEELDRDEQAL
                     DAPDILAIKGLGQFERFKAASAFRAMIENWHISDFHVSDARPSQEDGVAEHLSTRGDN
                     LALVANHLYENHPECFARVLDAMHRRVPGVSIVEPEQTIDGRLVLRFQDGSFRDPFIA
                     RHVSDGTIRMFAYLVLLNDPQPYPLLAVEEPENQLYPELLPELAEEFRAYAQRGGQVF
                     LSTHSPDFLNALTLDEIFCLRKENGFTTIRRASDSENLRALYDAGDLPGYLWKQRLFE
                     GLN"
BASE COUNT          331 a          536 c          490 g          309 t
ORIGIN      
        1 acttcctgcg tgaccgcgtc gaatccagcg acggcatttc atacgccagc gccagcgcag
       61 ccagatcacc gacataccag ctttccagct cctgacagat cagccggacc agcgtgtccg
      121 gacgcccgtg actggcacag gactcggaca agcgtcgctt gtggacatga caatcaggat
      181 gagcgtcatt gtcacgaacg acgacgaagc ggtcgtcagg tatctgccag ttgcgcagct
      241 tgcgcggcag gctgcgctcg agatcgcttt tgccttcatg cggcacgcac agaaactgct
      301 ggccggcagc ccagccggga aacatccggg gcagcaggcc attgagcagc gtggccatcg
      361 agggttcttc cagcaggaaa atgatccggc cccgcattca gttcagtccc tcgaacaggc
      421 gctgtttcca cagataaccc ggcagatcgc cggcgtcgta cagcgcacgc aggttttcgg
      481 aatcactggc gcggcggatc gtcgtgaagc cgttttcctt gcgcagacag aaaatctcgt
      541 cgagcgtcag cgcattcagg aaatccggcg aatgcgtcga gagaaacacc tgaccaccgc
      601 gctgcgcata ggcgcggaat tcctcggcca gctccggcag cagttccgga tagagctggt
      661 tctcgggttc ctcgaccgcc agcagcggat acggctgcgg atcgttgagc agcacgagat
      721 aggcgaacat cctgatcgtg ccatcggaaa catgacgggc gatgaacgga tcccggaagc
      781 tgccgtcctg aaagcgcagc accagccggc cgtcgatggt ctgctcgggc tcgacgatgc
      841 tgacgccggg cacccgccgg tgcatggcat cgagcacacg tgcaaaacat tccggatggt
      901 tctcgtacag gtgattggcc accagcgcca gattgtcgcc gcgcgtcgac agatgttcgg
      961 cgaccccgtc ttcctggctc ggccgggcat cggaaacatg gaagtcggaa atgtgccagt
     1021 tttcaatcat cgcccggaag gcactggcgg ccttgaagcg ttcgaactgc cccaggccct
     1081 tgatggcgag gatgtccggg gcatcgagag cctgctcgtc ccggtccagt tcctcgtccg
     1141 gccgattgaa atcctcttca ttggtgatcg cgtaaccgct gccgttcgag aaatccagaa
     1201 aatgaaacgg cctgccgtgg ctgccgcgct tgtagcgcag cacttcgcgc atcacgaccg
     1261 gcttgcgtaa tgaggcgtcc aggccgatct gcaactgata ggtgatcatc cgggaatgtc
     1321 cggcaatctc catccggaaa cgcaaggtca gttcgatcgg atcttcttca tgctcccggc
     1381 tggcaagctc acgatacccg ccgcgccggg cgatcgcctt gccgacgttc atcgacagcg
     1441 catccttcag aaacgagaac acgtcgaaca gcgtcgactt gccggtgccg ttggccccga
     1501 ccagcacgca caggcgcggc accgagcgga tgcgcacatg ccggaacacc cggtaattgc
     1561 ggatctcgat ttcttctatc tgcatggccg caccccggta atcctcatcg ccccatttcc
     1621 ggccgctctg gcttcagtgc gcctcgtccc agttcctgcc gctgcc
//
LOCUS       DAOQJH010000682         1657 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_66728_length_1657_cov_3.191011, whole genome shotgun sequence.
ACCESSION   DAOQJH010000682 DAOQJH010000000
VERSION     DAOQJH010000682.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1657)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1657)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1657
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66728_length_1657_cov_3.191011"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1195
                     /locus_tag="PLQ18_13230"
     CDS             <1..1195
                     /locus_tag="PLQ18_13230"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNE02662.1"
                     /translation="EQLISANPRDAGLRTDLGRLLLGIGRTDEAVRAWEQARTLDPRR
                     LEPRLALAGYRLEHREPLLAQTVASEAVAIDPGSFDALAALGMSQLALQQTAAARQSF
                     RQLGEAAGENLPRLLRAARHQLLAGAAQDAVDTLERARRIDPGQPDTLKALAEAQVSA
                     GQVAAAEATARTLLAQPEPPADAWGLLGEILMARGQAGEAAGVYEAGHARHPRSAYAV
                     AIWRAHAAAGAPARGLPALEAWAAQHPADPDARAALAIAWPQAGEWQKALGLYEALAA
                     ARPGDAMILNNLALLYLRTRDVRALATARRALAAAPGHPAVLDTLGWILVQQGQVAEG
                     LQQLQAALARDPLNPELLWHLGYTLHRLGRPAEARRPLEQALATGTGFDGAREARQLL
                     TALPR"
     gene            complement(1364..1513)
                     /locus_tag="PLQ18_13235"
     CDS             complement(1364..1513)
                     /locus_tag="PLQ18_13235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02663.1"
                     /translation="MRNRECNIRVADVMLANVRQPSFPAVNNGGSDSPPPQEAACQKI
                     EHRRR"
BASE COUNT          228 a          643 c          560 g          226 t
ORIGIN      
        1 cgaacagctg atcagcgcca atccgcgcga cgccggcctg cgcaccgatc tcggccggct
       61 gctgctcggc atcggtcgca cggacgaggc cgtgcgcgcc tgggaacagg cgcgcacgct
      121 cgatccgcgc cggctggagc cgcgcctggc cctggccggc taccggctgg agcaccgcga
      181 gccgctgctc gcccagacgg tcgcgagcga agcggtcgcg atcgaccccg gcagtttcga
      241 tgcgctcgcc gccctcggca tgagccagct cgcgctgcag cagactgcgg cggcccgcca
      301 gagcttccgc cagctcggcg aggccgccgg cgagaacctg ccgcggctgc tgcgcgccgc
      361 ccgccaccag ctgctggccg gtgcggcgca ggatgccgtt gacacgctcg aacgcgcacg
      421 gcgcatcgac cccggacagc cggacacgct gaaggcgctg gccgaagccc aggtctcggc
      481 cgggcaggtg gccgcggccg aagccacggc ccgcaccctg ctcgcgcagc ccgaaccgcc
      541 ggccgatgcc tggggactgc tcggcgagat cctgatggca cgcggacagg cgggcgaggc
      601 ggccggcgtc tacgaggccg gccatgcccg ccatccgcgc agcgcctatg cagtcgcgat
      661 ctggcgtgcc cacgccgccg ccggcgcccc ggcccgcggc ctgccggcgc tcgaagcctg
      721 ggcagcgcag catccggccg atccggacgc gcgtgcggca ctggcgatcg cctggccgca
      781 ggccggcgag tggcagaagg cgctcggcct gtacgaagcc ctcgccgccg cccgccccgg
      841 cgacgcgatg atcctcaaca acctcgcgct gctgtacctg cgcacccgcg atgtccgcgc
      901 gctggccacg gcccggcgcg cgctggccgc cgcccccggt cacccggccg tgctcgatac
      961 gctgggctgg attctggtgc agcagggtca ggtggcggag ggtctgcagc agttgcaggc
     1021 ggcgctggcg cgcgacccgc tcaacccgga actgctctgg cacctcggct atacgctgca
     1081 ccgcctcggc cgcccggccg aagcgcgccg gccgctcgaa caggcgctgg cgaccggcac
     1141 cggcttcgac ggcgcccggg aagcccggca actgctcacc gccctgcccc gctgacgcag
     1201 cccgccggct tcgatcccgc gctgcgcaag cagccggcgt tcgaagccgg tgcgggcgca
     1261 gcccggcgca tgcccgccgt ccctcgccgg tgacccggcg cccggccatg gaccctgcgg
     1321 tccgccggcg gccatctgca aaccgcaagc tttcctgttc cggtcaccgg cggcggtgtt
     1381 caattttctg acacgccgcc tcttgcggtg gcggcgaatc cgacccgccg ttattcacgg
     1441 ccgggaatga cggctgccgg acattcgcca gcatcacgtc ggccacccgg atattgcatt
     1501 cgcgattgcg catgccggac atccattacg ccaggcggga cagtcagatt attttacaca
     1561 ccggcttccg gacaaaaatg ggcatcgacc tttgctgcat ttgcatttcg gcttccactg
     1621 acgtgcattc tgcaaattcc ccgtcaggat ttgcatt
//
LOCUS       DAOQJH010000683         1656 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_66791_length_1656_cov_5.013117, whole genome shotgun sequence.
ACCESSION   DAOQJH010000683 DAOQJH010000000
VERSION     DAOQJH010000683.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1656)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1656)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1656
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66791_length_1656_cov_5.013117"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..509)
                     /locus_tag="PLQ18_13240"
     CDS             complement(<1..509)
                     /locus_tag="PLQ18_13240"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNE02664.1"
                     /translation="MRVLAVGPQRLAPVRALTRLPLRVQTGADCWLAFLPETGDCPAG
                     AAALHPDGALWIAVVPALRRRGVGRALHATAMAAAQAQGLTRVVSWRGTDTPEAAAFC
                     AAVGLQVVRTIETFETTLANTVRFFGRYRDRLLARGRLPAGILEQNDGEVPWGAYGAA
                     LIEAFGPPAA"
     gene            complement(1043..>1656)
                     /locus_tag="PLQ18_13245"
     CDS             complement(1043..>1656)
                     /locus_tag="PLQ18_13245"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="PEP-CTERM proteins occur, often in large numbers,
                     in the proteomes of bacteria that also encode an
                     exosortase, a predicted intramembrane cysteine proteinase.
                     The presence of a PEP-CTERM domain at a protein's
                     C-terminus predicts cleavage within the sorting domain,
                     followed by covalent anchoring to some some component of
                     the (usually Gram-negative) cell surface. Many PEP-CTERM
                     proteins exhibit an unusual sequence composition that
                     includes large numbers of potential glycosylation sites.
                     Expression of one such protein has been shown restore the
                     ability of a bacterium to form floc, a type of biofilm;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HNE02665.1"
                     /translation="VDLVPDLVTANNATIGNFLDLNGDGSDDFAFGHDATDTGSDGQT
                     DAIAVVFSDPSFVMIANDAGFPSATRFGTGSTIDAAAFADANKVGVLAIDDFDLNGGG
                     VRAQAGNWYNGGAGTIGYVGFAMTGLPGDVVGTDYHFGWMRVGVSAYDNTSNSLVITA
                     YDYAYESVAGVGITIPEPAGWALFAMGGTALAARRRRRTATTA"
BASE COUNT          269 a          555 c          591 g          241 t
ORIGIN      
        1 gccgcaggcg gtccgaaggc ctcgatcagc gccgcgccat aggcgcccca gggcacctcg
       61 ccatcgttct gctccaggat gccggccggc agccgcccgc gcgccagcag gcggtcgcgg
      121 tagcggccga agaagcgcac ggtgttcgcg agcgtggttt cgaaggtttc gatggtacgc
      181 accacctgca ggccgacggc tgcgcagaag gccgcggctt ccggtgtgtc cgtcccgcgc
      241 cagctcacca cgcgcgtcag cccctgcgcc tgcgctgccg ccatggccgt cgcatgcagc
      301 gcccggccga cgccgcgccg gcgcagggcc ggcacgaccg cgatccacag cgcgccatcc
      361 ggatgcagcg cggcagcccc ggccgggcag tcgccggtct cgggcaggaa ggccagccag
      421 cagtcggcgc cggtctgcac gcgcagcggc agacgcgtca gcgcccgtac cggcgcgagg
      481 cgctgcggcc caacggccag gacccgcatc agaggaacga cgcccgcccg cagggcaggc
      541 aggacatgtg cacgcggacg gcacacccgg ccctgtccgg gccgggacgg agcagtgcgc
      601 aggacggacc ggattgcggc aggccgcctt gcggatcaac gggcaggagg gacatggcgg
      661 gggcgtcctt gtgctgttgg gcggaatgaa ggcgcgtgtg ccgggggagt ccgtggctcg
      721 atccgggctt ctgaccggca cacctgacag cacggtgctc aggagccggt ggcggttgat
      781 gcactgcaac gggtgttaat cttccccgaa taattgtcaa gtttggcttg catgccgatt
      841 tttttttcgg ccgcatgcac gattgccaaa gccggacgac tacggcccgc cggcgtcggc
      901 gtcgcagtca gggcgttgat ccgaagtgaa tccggtttcc cggaagggcg ggcagacgtg
      961 cgaccgggtc gccggacgca gccgcagcga tctgccagac ccggccggca agccctgctt
     1021 gccggccggc agccggagtc gatcaggcgg tcgtggccgt ccggcgacgc cggcgcgcgg
     1081 cgagcgccgt gccacccatc gcgaacagcg cccagcccgc cggctcgggg atcgtgatgc
     1141 cgaccccggc gaccgactcg taagcgtagt cgtaagcggt gatcaccagc gagttgctgg
     1201 tgttgtcgta ggcgctgacg ccgacgcgca tccagccgaa gtgatagtcg gtaccgacca
     1261 cgtcaccggg caggccggtc atcgcgaatc cgacgtagcc gatcgtgccg gcgccgccgt
     1321 tgtaccagtt gccggcctgc gcgcgcacgc cgccaccgtt cagatcgaaa tcatcgatgg
     1381 ccaggacgcc gaccttgttg gcatcggcga aggcggcggc gtcgatggtg ctgccggtac
     1441 cgaaacgcgt tgcgctcgga aagccggcat cgttggcgat catgacgaac gacggatcac
     1501 tgaatacaac tgcgatcgcg tcggtctggc catcgctgcc ggtatctgtt gcgtcatgcc
     1561 cgaaggcgaa atcatccgaa ccgtcgccgt tgaggtcgag aaaattaccg atggtcgcat
     1621 tgttggcggt gacgaggtcg ggaacgagat cgacgt
//
LOCUS       DAOQJH010000684         1656 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_66814_length_1656_cov_2.899438, whole genome shotgun sequence.
ACCESSION   DAOQJH010000684 DAOQJH010000000
VERSION     DAOQJH010000684.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1656)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1656)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1656
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66814_length_1656_cov_2.899438"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(33..>1656)
                     /locus_tag="PLQ18_13250"
     CDS             complement(33..>1656)
                     /locus_tag="PLQ18_13250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009150709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HNE02666.1"
                     /translation="LICWVLVALAAQIGLLPPVPVPRSSFVLTGLLLLLFLGAPRFVY
                     RWIKDHGLYYRPGQRVLIVGAGDAGVSLLRELQRERDGEYEPVGFVDDKDNRQGRELH
                     GLRVLGRTQDVAAIADRYAIDMIFVAMPSLGGERMARVLRLCEASGRPVRVVPRLDEL
                     LSGRAQIRELREVSLEDLLGREEVTLDWETIGHCVAGRTVLVSGGGGSIGSELCRQLA
                     RLGPARLLIVDAGEFNLYRIELELRRDWPEVSLQVFLLDVTDAVGVGQVFATHRPELV
                     FHAAAYKHVPMLEDQPSTAIGNNVFGTMTLAQAADCHGADAFVLISTDKAVNPTNVMG
                     ASKRLAEMYCQNLAARSHTHFITVRFGNVLGSAGSVVPLFREQIARGGPVTVTHPEIE
                     RYFMTIPEAAQLILQAAAVGRGGEIFVLDMGAPIRIATLAEQMIQLSGKRPHQDIRIE
                     FTGLRPGEKLYEELFHEREQLQPTVYPKLLLAQRRTLDWGVFNRALEALRADCRERRD
                     AEVLAAMHVLVPEYRTSGSPGTRDGPVQATGT"
BASE COUNT          265 a          593 c          554 g          244 t
ORIGIN      
        1 gctcgaactg gtgctcaggc gctgatcgcc gctcaggtgc cggtggcctg taccggtccg
       61 tcccgggtcc ccgggctgcc ggatgtccgg tactccggca ccagcacgtg catcgccgcc
      121 agcacctcgg catccctgcg ctcccggcag tcggctcgca atgcctccaa tgcccggttg
      181 aacacgcccc agtccagcgt gcggcgctgc gcgagcagca gcttcgggta gacggtcggc
      241 tgcagttgct cgcgttcgtg gaacagctcc tcatagagct tctcgcccgg gcgcaggccg
      301 gtgaactcga tgcggatgtc ctgatgcggg cgcttgcccg agagctggat catctgctcg
      361 gccagcgtcg cgatgcggat cggtgcgccc atgtcgagca cgaatatctc gccgccgcgc
      421 ccgaccgccg ccgcctgcag gatcagttgc gcggcctcgg gaatggtcat gaagtagcgc
      481 tcgatctcgg gatgcgtgac cgtcaccggc ccgccgcgcg cgatctgctc gcgaaacagc
      541 ggcacgacgc tgccggccga gccgagcacg ttgccgaagc gcacggtgat gaagtgcgtg
      601 tggctgcggg cggcgaggtt ctggcagtac atctcggcca gccgcttgct cgcccccatg
      661 acgttggtcg gattgaccgc cttgtcggtc gagatcagca cgaaggcgtc ggcgccatgg
      721 caatcggcgg cctgcgccag cgtcatcgtg ccgaacacgt tgttgccgat cgccgtcgaa
      781 ggctggtctt cgagcatcgg cacgtgcttg taggcggcgg catggaacac cagctcgggc
      841 cggtgcgtgg cgaacacctg cccgacgccg acggcatccg tgacatcgag caggaacacc
      901 tgcagcgaaa cttcgggcca gtcgcggcgc agttcgagtt cgatgcggta caggttgaac
      961 tcgccggcat cgacgatcag cagccgcgcc ggcccgagcc gggcgagctg gcggcagagc
     1021 tcggaaccga tcgagccgcc gccgccgctg accagcaccg tgcgcccggc cacgcagtgt
     1081 ccgatcgtct cccagtcgag cgtgacctcc tcgcgcccga gcaggtcttc gagcgagacc
     1141 tcgcgcagct cgcgaatctg cgcccgcccg ctcagcagct catcgagccg aggcacgaca
     1201 cgcaccggcc ggccgctcgc ctcgcagagc cggagcacgc gcgccatgcg ttcgccgccg
     1261 agcgagggca tggccacgaa gatcatgtcg atcgcgtacc gatcggcgat ggcagccacg
     1321 tcctgcgtac gcccgagcac ccgcaggccg tgcagttcgc ggccctggcg gttgtccttg
     1381 tcgtcgacga aaccgaccgg ttcgtattcg ccgtcgcgct cgcgctgcag ttcacgcagc
     1441 agcgacacgc cggcgtcccc ggccccgaca atcagtacgc gctgcccggg ccggtagtac
     1501 aggccgtgat ccttgatcca gcgatacacg aagcgcggcg ccccgagaaa cagcagcagc
     1561 agcaggccgg tcagcacgaa ggacgagcgc ggcaccggca ccggcggcag caggccgatc
     1621 tgcgcggcca gcgccaccag cacccagcag atcagc
//
LOCUS       DAOQJH010000685         1645 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_67667_length_1645_cov_4.461006, whole genome shotgun sequence.
ACCESSION   DAOQJH010000685 DAOQJH010000000
VERSION     DAOQJH010000685.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1645
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67667_length_1645_cov_4.461006"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(245..1600)
                     /locus_tag="PLQ18_13255"
     CDS             complement(245..1600)
                     /locus_tag="PLQ18_13255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HNE02667.1"
                     /translation="MDWNQLLSRRRLGIRHRSSAEDTQRTDFQRDFDRIVFASAFRRM
                     QDKTQVFPLSSNDYVRTRLTHSLEVSSVARSLGNRVGEVVIARHGLTQVVPADFGAIV
                     AAAALAHDLGNPPFGHAGEDGIRQFFEETTLGRSLIGRMHPAEQQDLLRFEGNAQGFR
                     VVARLQSPDNFGGMQLTLATLGSFTKYPRGSLLDADPAVGGIAFKKFNYFQADAALFD
                     EVATGLGLDPVLPGAWRRHPLAFLLEAADDICYRIVDLEDAVRLRQLEHGEVLDLLLP
                     LVGLAADSPRLKQLTRPKERIEYLRAKAIGRLIDEVVTLFLDCEAEILAGRYGLELLD
                     EVPSRAPMLEMKELARRRVYIGQPVVEVQVAGFEVLGGLLAAFAGAVTDVAERGGRAT
                     ALNRMLMHLLPEQFVGPGHEPVADAYLRTLKIIDFISGMTDSYAVSLFKRITGMSLPM
                     Q"
BASE COUNT          273 a          591 c          562 g          219 t
ORIGIN      
        1 catcagcccg atgcccgccc gcaaccggct gccggcgccg tccacaccgc gccgctccgg
       61 tccccgccgc cccatggcac ggtgcgcacc cgatcacagc ccgcaccgga gtccgccccg
      121 catgccgtgc ccgcccctgc cccgtacccc gtaccgcacc cgccgcggcc cggcgcgaat
      181 ccggcacgcc cccgatgaac ccgcagcgca gaccgcacgg gtgcagcgcg gcaaccgcat
      241 cggcttactg catcggcagc gacatgccgg tgatgcgctt gaacaggctg acggcgtagg
      301 aatcggtcat gccggagatg aagtcgatga tcttcagcgt gcgcaggtag gcatcggcca
      361 ccggctcatg cccggggccg acgaactgct cgggcagcag gtgcatcagc atgcggttca
      421 gtgccgtcgc ccgcccgccg cgctcggcga catcggtgac ggcaccggcg aaggccgcga
      481 gcaggccgcc aagcacctcg aagccggcga cctgcacctc gacgaccggc tggccgatgt
      541 agacacgccg gcgcgcgagt tccttcatct ccagcatcgg cgcgcggctc ggcacttcat
      601 cgagcagttc gagcccgtag cggccggcga ggatctcggc ctcgcagtcg aggaacagcg
      661 tcacgacctc atcgatcagc ctgccgatcg ccttggcgcg caggtactcg atgcgctcct
      721 tcggccgggt cagctgcttc aggcgcggcg aatcggccgc aagccccacg agcggcagca
      781 gcaggtccag cacctcgccg tgttcgagct ggcgcaggcg cacggcgtcc tcgagatcga
      841 cgatgcggta gcagatgtca tcggccgctt cgagcaggaa agccagcgga tggcgccgcc
      901 aggcgccggg aagcaccgga tcgagcccga gcccggtcgc aacctcgtcg aacagcgccg
      961 catcggcctg gaagtagttg aacttcttga aggcgatgcc gccgactgcc ggatcggcgt
     1021 cgagcagcga accgcgcgga tacttcgtga agctgccgag ggtcgccagc gtcagctgca
     1081 tgccgccgaa gttgtccggg ctctgcaggc gcgcgacgac gcgaaagccc tgggcgttgc
     1141 cctcgaagcg cagcaagtcc tgctgctcgg ccggatgcat gcgcccgatc agcgaacgcc
     1201 cgagcgtcgt ttcctcgaag aactggcgga tgccgtcctc gccggcatgg ccgaacggcg
     1261 gattgccgag atcgtgcgcg agcgcggccg ccgcgacgat cgccccgaaa tcggccggca
     1321 cgacctgcgt cagcccgtgg cgcgcgatca cgacctcgcc gacccggttg ccgagcgagc
     1381 gcgcgacgct ggacacctcc agcgaatgcg tcaggcgcgt gcgcacgtaa tcgttcgacg
     1441 acagcggaaa gacctgcgtc ttgtcctgca tgcgccggaa cgccgaggca aacacgatgc
     1501 ggtcgaagtc gcgctggaag tcggtgcgct gcgtgtcctc ggcagaggat cggtggcgga
     1561 tgccgaggcg gcggcgactg aggagctggt tccagtccat cgacgggaac tccgggtgac
     1621 ggcgggggcc ggcagcgtag cgcgc
//
LOCUS       DAOQJH010000686         1640 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_68058_length_1640_cov_5.081388, whole genome shotgun sequence.
ACCESSION   DAOQJH010000686 DAOQJH010000000
VERSION     DAOQJH010000686.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1640
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68058_length_1640_cov_5.081388"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            67..684
                     /locus_tag="PLQ18_13260"
     CDS             67..684
                     /locus_tag="PLQ18_13260"
                     /inference="COORDINATES: protein motif:HMM:NF019960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HNE02668.1"
                     /translation="MVRRTKAEAEQTRSLILDTAERLFFTKGVSRTTLAEIATAAGLT
                     RGAIYWHFANKLELFNAMHERVALPFTEAMEKLLHAEDPLAALRERCVLDLQDIAACP
                     QLQQVLAVLLHKCEYVDELGELCLRFDQQNKCFVEHSETVLARAHALGQLRDGVEPRR
                     AAIALHNYMLGTISDWLFAPDLRYRLADEAGPLVDLFFAGLERPA"
     gene            complement(785..>1640)
                     /locus_tag="PLQ18_13265"
     CDS             complement(785..>1640)
                     /locus_tag="PLQ18_13265"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HNE02669.1"
                     /translation="DRTVPRLVIVNIPSYKLRAFESGQLALSSNVVVGRPKRPTPELS
                     VFMKAVKYNPTWTPPPTIIKEDLIPLFGRDPSAVTRKGLVVLDRGGRQIPPDAIGPIS
                     AAGFHAAGYRMYQPAGDSNALGKLKFEITDTPSVYLHDTNHRNDFGKPVRALSSGCVR
                     VHQYRPLAAWLMGLSEDEIQKRINRRRTVVEPSPQIPIYLVYRPAEAIDGHVVYYSDI
                     YGKLNGPLPKPKPIPVIEPAPAPEATPAAPSADPSVPPVAPVTPAAVPVATTPAVATA
                     SEPAPATR"
BASE COUNT          259 a          554 c          574 g          253 t
ORIGIN      
        1 gaagcgatac actgtccgca accggcaccg actccgccgt tttcccggga ttcaccccgc
       61 accgacatgg tcagacgcac caaggccgag gccgagcaga cgcgctcgct gatcctcgac
      121 accgccgaac gcctgttctt cacgaagggc gtctcgcgca ccacgctcgc cgagatcgcg
      181 acggccgccg ggctgacgcg cggcgcgatc tactggcatt tcgcgaacaa gctcgaactg
      241 ttcaacgcga tgcacgaacg cgtcgcgctg ccgttcaccg aggcgatgga aaagctgctg
      301 cacgccgaag acccgctggc ggcgctgcgc gagcgctgcg tgctcgacct gcaggacatc
      361 gccgcctgcc cgcaattgca gcaggtgctg gcggtgctgc tgcacaagtg cgagtacgtc
      421 gacgaactcg gcgagctgtg cctgcgcttc gaccagcaga acaagtgctt cgtcgagcac
      481 agtgaaaccg tgctcgcacg cgcgcacgcg ctcgggcagt tgcgcgatgg tgtcgaaccg
      541 cggcgcgcgg cgatcgcgct gcacaactac atgctcggca cgatcagcga ctggctgttc
      601 gcgccggacc tgcgctaccg gctcgccgac gaggccggcc cgctggttga tctgttcttc
      661 gccggcctcg aacgtccggc ctgacgcctg cggccgcgcg ccctgcgccg gaggccggga
      721 aaaaacgaag gccgcaccgg gcatggaccg gtgcggcctt cgtcttctgg cagaggccgg
      781 gttttcagcg cgtcgccggc gccggctcgc tcgccgtagc cacggccggc gtcgtcgcca
      841 ccggcacggc ggccggcgtc accggcgcga ccggcggcac cgacggatca gcggacggcg
      901 ccgccggcgt ggcttccggg gccggcgccg gctcgatgac cgggatcggc ttcggtttcg
      961 gcagcgggcc gttcagcttg ccgtagatgt cgctgtaata aacgacgtga ccgtcgatgg
     1021 cctcggccgg gcggtagacc agatagatcg gaatctgcgg cgaaggctcg acgacggtgc
     1081 ggcggcggtt gatgcgcttc tggatctcat cctcgctcag gcccatcagc caggcggcga
     1141 gcggccggta ctggtgcacg cgcacgcagc ccgaggacag cgcccgcacc ggcttgccga
     1201 agtcgttgcg gtggttggtg tcgtgcaggt agacgctcgg cgtgtcggtg atctcgaact
     1261 tcagcttgcc gagcgcgttg gaatccccgg ccggctggta catgcgatag ccagcggcgt
     1321 ggaagccggc ggcgctgatc ggaccgatcg catcgggcgg aatctggcgg ccgccgcgat
     1381 cgagcacgac cagccccttg cgcgtgaccg ccgacggatc acgaccgaac agcgggatca
     1441 ggtcttcctt gatgatggtc ggcggcggcg tccaggtcgg gttgtacttg accgccttca
     1501 tgaacaccga cagctccggc gtcgggcgct tgggccgacc gacgacgacg ttggagctga
     1561 gcgcgagctg accggactcg aaggcgcgca gcttgtagga cgggatgttg acgatcacca
     1621 gccgcgggac cgtgcgatcg
//
LOCUS       DAOQJH010000687         1640 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_68061_length_1640_cov_4.388013, whole genome shotgun sequence.
ACCESSION   DAOQJH010000687 DAOQJH010000000
VERSION     DAOQJH010000687.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1640
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68061_length_1640_cov_4.388013"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="PLQ18_13270"
     CDS             complement(<1..188)
                     /locus_tag="PLQ18_13270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02670.1"
                     /translation="MIYQIRHYHGPAVTTDRIRAFKSIGKTLRRINFGGRTDVSGLDR
                     ARGLLNNWSEAIIQRWVM"
     gene            complement(185..>1640)
                     /locus_tag="PLQ18_13275"
     CDS             complement(185..>1640)
                     /locus_tag="PLQ18_13275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02671.1"
                     /translation="IARGHDPDRVGAWVCGQDCATAASEARGAWKAATGEVYGMVKAA
                     AWTAPKPGFDPDELARVDAEHTKQSARLAALREEATTAREAERARDRLAGLRAHANDQ
                     ILIERAGEAETLRARLAEAQSAPLPPAAAQDEAVRNATADLSEARRAVTAAAAEKLNA
                     EAAQTITPCPHCAGPLLVADGAIRAADPAAPARDQGRIAAAVRALGMAKEAEAAASKR
                     HAEAVRAADSRYSTALSARDANVAAASAALTTAEDAARTLASARAALEAADLPPPPRP
                     SAELDAEIAELVARVEALDQQRKALREAEKAIARADDITARAAAAYADVQAWVALDDA
                     VEALPNEMLAAGLAPLNALLRELAGHARPDLFVTPRGLPLVPSVSPDMSIRCGHRPYH
                     AFSDSERWRIDALLGAAVASLSGHRLLVLDEADMLQPGLRPSLLGWLDALVTAGHLET
                     ALVMATTREPFRVPKHVAQFRLPMVDAAGEEAAA"
BASE COUNT          205 a          590 c          602 g          243 t
ORIGIN      
        1 tccataaccc atcgctgaat gatcgcctcg gaccagttgt tcagcagccc ccgcgctcgg
       61 tcgagtccgg acacgtcagt ccggccgccg aagttgatcc ggcgcagggt tttgccgatg
      121 gacttgaagg cgcgaatgcg gtcagtagtc acggcagggc cgtggtagtg gcggatttgg
      181 tagatcatgc cgccgcctcc tcgccagccg cgtccaccat cggcaaccgg aactgcgcca
      241 catgcttcgg cacgcggaac ggctcgcgcg tcgtcgccat cacaagtgcg gtttcgaggt
      301 gcccggccgt caccagtgca tccagccacc cgagcagcga cgggcgcagg ccgggttgca
      361 gcatgtcggc ctcgtccagc accagcagcc ggtgtccgga caggctcgcg accgcagcgc
      421 cgagcagcgc gtcgatgcgc cagcgctccg agtcggaaaa cgcgtgatac ggccggtgcc
      481 cgcagcggat cgacatgtcc ggcgacactg acggcaccag cggcaggccg cgcggcgtga
      541 cgaacaggtc cggacgggcg tgtccggcga gctcgcgcag cagggcattc agcggcgcga
      601 ggccggccgc cagcatctcg ttcggcagcg cctcgaccgc atcgtcgagc gcgacccagg
      661 cctgcacgtc ggcatacgct gcggcagcgc gggccgtgat gtcgtcggcg cgggcgatgg
      721 ccttctcggc ttcgcgcagc gccttgcgct gctggtcgag cgcttcgacg cgggcgacaa
      781 gctcggcgat ctcggcgtcg agttcggcgg acgggcgggg cggaggcggg aggtcggcgg
      841 cttcgagcgc ggcgcgggcg gatgccagcg tgcgggcggc atcctcggcg gtggtcagtg
      901 cagcgctggc ggcagcgacg ttggcgtcac gggctgacag ggcggtgctg tagcgactgt
      961 cggcagcacg cacggcttcg gcgtgacgct tgctcgctgc ggcttccgcc tccttcgcca
     1021 tgccgagcgc gcgcaccgcg gcagcgatgc ggccctgatc gcgcgccggg gccgccgggt
     1081 ccgcggcacg gatggcgccg tccgcaacca gcagcgggcc ggcgcagtgc gggcagggcg
     1141 tgatggtctg cgcggcctct gcgttgagct tctcggcagc cgccgcggtg actgcgcgcc
     1201 tcgcctcgga cagatcggcg gtcgcgttgc gcacggcttc gtcctgagcc gctgccggcg
     1261 gaagcggcgc gctctgggcc tctgccagcc gagcgcgcag cgtttcggcc tcgccggcgc
     1321 gctcgatcag aatctggtca ttggcatgtg cccgcagccc tgccagccga tcacgcgccc
     1381 gctcggcctc gcgggcggtg gtagcctctt cgcgcagggc cgccagccgc gcggactgct
     1441 tggtgtgctc ggcatcgacg cgggccagtt cgtccgggtc aaatccgggc ttcggcgcgg
     1501 tccatgccgc ggccttgacc atcccgtaca cctcgcccgt ggccgccttc cacgcgccgc
     1561 gcgcctcgct cgcggccgtg gcgcagtcct gcccgcacac ccacgccccg acgcgatccg
     1621 ggtcgtgccc gcgggcgatc
//
LOCUS       DAOQJH010000688         1637 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_68310_length_1637_cov_3.379899, whole genome shotgun sequence.
ACCESSION   DAOQJH010000688 DAOQJH010000000
VERSION     DAOQJH010000688.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1637)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1637)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1637
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68310_length_1637_cov_3.379899"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..240)
                     /locus_tag="PLQ18_13280"
     CDS             complement(<1..240)
                     /locus_tag="PLQ18_13280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02672.1"
                     /translation="MSAAVAFSPAITPSAYAGPSVRGSEEAAMPAQTGSPASAAAAST
                     DTRTEVAELVAIDARVRAHEAAHRAAAGELARGGSY"
     gene            518..964
                     /locus_tag="PLQ18_13285"
     CDS             518..964
                     /locus_tag="PLQ18_13285"
                     /inference="COORDINATES: protein motif:HMM:NF020544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1877 family protein"
                     /protein_id="HNE02673.1"
                     /translation="MSMNGFFRAFPADAYHEMLRNPARIDPLIWDEDQSADSTDVETA
                     WDVLQRLLDGAGFDFDQQVDQALSNGACFIAPETVLRHAASLSGWSHDKLLEALENVG
                     DEELYHLDLFREDEDGQQSLLEEFDKLAAFYARAAENRHGVIFYLA"
     gene            complement(974..>1637)
                     /gene="rlmJ"
                     /locus_tag="PLQ18_13290"
     CDS             complement(974..>1637)
                     /gene="rlmJ"
                     /locus_tag="PLQ18_13290"
                     /inference="COORDINATES: protein motif:HMM:NF016278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HNE02674.1"
                     /translation="YRDGIARLWSRADLEPELAAYVAAVRSENPDGRLSRYPGSPALI
                     RQRLRDRDRLIACELHPGDQPSLKALFAADRRIAVHKRDGYEAAGAFLPPPERRGLLF
                     VDPAYELAGEFERLLKLVGQVHRRWREGTVAIWYPILDRAPSERFCRALAASGVPALL
                     RIELGLAPYDTPLGMKGSGVIVCNPPYQLDVAARRWLAQLQGILSDSGSGETRVEWLT
                     TA"
BASE COUNT          252 a          565 c          577 g          243 t
ORIGIN      
        1 ataggagccg ccgcgcgcca gctcgccggc cgcggcccgg tgggcggcct cgtgggcgcg
       61 cacgcgggca tcgatggcga cgagttcggc gacttcggtg cgggtgtccg tcgaggcggc
      121 agcggcgctc gccggactgc cggtctgcgc cggcatcgcc gcctcctcgc tgccgcgcac
      181 ggacggaccg gcgtaggcgg agggcgtgat ggcagggctg aaggcaacgg cggcagacat
      241 ctcgggcacc tgcggctgaa cggggtcagt gtagcggccg gggcgcagcc cggcaggtcg
      301 tgccgccggg ccgtcgtgcg gagatacgcc tgaagcccgg ttgccgtcct gccgaaaagg
      361 cggcgcactc ggcagcagca tgccacgctg attcccggcc ccgtaccccc tgcggcgacg
      421 gtgcacgccc gggttcggtc cggtccgcac ctccggacgc atcccccggc gctttccagc
      481 ctttcccata cctgccgagg attccaggag tctgccgatg agcatgaatg gatttttcag
      541 ggccttcccg gccgatgcct atcacgaaat gctccgcaac ccagcccgga tcgacccgct
      601 tatctgggac gaggatcaat cggctgattc gaccgatgtc gaaaccgcct gggatgtcct
      661 gcaacgcctg ctcgacggcg ccggattcga ttttgaccag caggtcgatc aggcgctgag
      721 caacggcgcc tgcttcatcg caccggaaac cgtgctccgg catgccgcct ccctgtccgg
      781 ctggtcgcat gacaagcttc tcgaagcgct ggaaaacgtc ggcgacgagg agctgtacca
      841 tctggacctg ttcagggagg acgaggacgg ccagcagtcg ctgctggaag agttcgacaa
      901 gctggcggcc ttctacgcga gagccgcgga aaaccggcac ggcgtgatct tctacctggc
      961 ctgagcagcg gcctcaggcc gtcgtcagcc attcgacgcg cgtctcgccg gagccgctgt
     1021 ccgacaggat tccctgcagt tgcgccagcc agcgccgggc cgcgacatca agctgatacg
     1081 gcggattgca gacgatcacg ccgctgccct tcatgccgag cggcgtgtcg tagggcgcga
     1141 gccccagttc gatgcgcagc agcgccggca cgccgctggc ggcgagggca cggcagaagc
     1201 gttcggacgg cgctcggtcg aggatcggat accagatcgc caccgtcccc tcgcgccagc
     1261 gccggtggac ctgcccgacc agcttcagca gccgttcgaa ttcgccggcc agctcgtagg
     1321 ccggatcgac gaacagcagc ccgcgccgct cgggcggcgg caggaaggca ccggcggcct
     1381 cgtagccgtc gcgcttatgc accgcgatgc gccggtccgc ggcgaacagc gccttcagcg
     1441 acggctgatc gcccggatgc agctcgcagg cgatcagccg gtcccggtcg cgcaggcgct
     1501 ggcggatcag cgccggcgag ccggggtagc ggctgagccg gccgtcgggg ttttcgctgc
     1561 gcacggcagc gacgtaggcg gcgagttccg gttcgagatc ggcgcgtgac cacagccggg
     1621 caatgccgtc gcggtat
//
LOCUS       DAOQJH010000689         1616 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_70035_length_1616_cov_3.755285, whole genome shotgun sequence.
ACCESSION   DAOQJH010000689 DAOQJH010000000
VERSION     DAOQJH010000689.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1616
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70035_length_1616_cov_3.755285"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            542..>1616
                     /locus_tag="PLQ18_13295"
     CDS             542..>1616
                     /locus_tag="PLQ18_13295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HNE02675.1"
                     /translation="MRMTTRFIASLLAVLAVVAVALSPLIGGLISRWFQRDIEIRSGL
                     VFHTLEAPLAERLGRHDADGIRTLFDDVDRDERVAGVGWCDARNVLVHRSRRMPDGFS
                     CRFAPATTGEPVFRSETSFGRQLLVGAFPLAGGRLVIVHDIGYITDRSASAQGYLVVS
                     LIVLAALISMVTVLVARLTLRDWLRTVRDSFAQPVASAAKPSSADLAPLVDEIRQRLR
                     ALDGVRRSVDDFRLNWAPETLAAILDRELPDSEVIVVSNREPYIHNLVDGRIDLQIPA
                     SGLVSALEPVIRACSGTWIAHGSGSADNLSVDAHDHIAVPPDAPAYTLRRVWLSEAEQ
                     DGYYYGLANEGLWPLCHIAHVRPT"
BASE COUNT          231 a          540 c          545 g          300 t
ORIGIN      
        1 gcatacgcga cgcgggcggc agcttcgttg ccgtcgagga tttcgggaca ggcggggtgc
       61 ggcagcagcg gggcgttcat gacgccacct ccgggggcaa gtcagggatc agacctgacc
      121 ttgcctgtcg gccggggggg tgggacatga tctgcgtcag cgggcgatct gccggcatgt
      181 ggctgacgtt tcgtaatgcg gagtaaaaaa gacgtcacat ccggccggaa gctctggatt
      241 tcatgctgat gtatcgaatt gcgaaatatg ttccgatatg cggaacaaaa tgtcgggtcg
      301 attgcatgac atgcatggat cacgatcgct gtccggcgtg cgggcggcat ggtgcagtgc
      361 ctcgtcagga tgtgtcttgc ggcacgtttg ctgctttaat cccggggcgc ccctgacccg
      421 gtctgtccgc cggcagcctt ggcacctgct tcagacttcc ggccccggcg gtcgtgtcag
      481 gccgatccgc cccgtgcgcg gacgtaccgc gaccgtgcac gcctcccccc ggaagctgcc
      541 catgcgcatg accacccggt tcatcgcatc gctgctggcg gtgctggccg tcgtcgccgt
      601 ggccctgtcg ccactgatcg gcggcctgat ctcgcgctgg ttccagcgcg acatcgaaat
      661 ccgttccggc ctggtgttcc acacgctcga ggccccgctg gccgagcgcc tcgggcgtca
      721 cgacgccgat ggcatccgta cgctgttcga cgatgtcgat cgtgacgaac gtgtggccgg
      781 cgtcggctgg tgcgatgccc gcaacgtgct cgtgcatcgc agccggcgca tgccggacgg
      841 cttctcgtgc cgtttcgcgc cggcaaccac cggcgagccg gtcttccgca gcgagacctc
      901 gttcggccgg cagttgctgg tcggtgcgtt tccgctcgcc ggcggccggc tcgtgatcgt
      961 gcacgacatc ggctacatca ccgaccgcag cgccagtgcc cagggctatc tggtcgtgtc
     1021 gctgatcgtg ctcgcggcgc tgatctcgat ggtgacggtg ctggtcgcac ggctgacgct
     1081 gcgcgactgg ctgcgcaccg tgcgtgacag cttcgcgcag ccggtggcga gcgcagcgaa
     1141 gccgtcctcc gccgatctgg cgccgctggt cgacgagatc cgccagcggt tgcgcgcgct
     1201 cgacggcgtg cgccgcagcg tcgatgactt ccggctgaac tgggcaccgg agacgctggc
     1261 agcgatcctc gatcgtgagc tgccggattc cgaggtcatc gtcgtttcca accgcgagcc
     1321 ctacatccac aacctcgtcg acggccggat tgatctgcag attccggcga gcggcctggt
     1381 ctcggcgctg gagccggtga tccgtgcctg ttccggcacc tggatcgcgc acggcagcgg
     1441 aagcgccgat aacctgagcg tcgatgcaca cgaccacatt gccgtgccgc ccgatgcacc
     1501 ggcctacacg ctgcgccgcg tctggctcag cgaggccgag caggacggct actactacgg
     1561 gctcgccaac gagggcctct ggccgctctg ccacatcgcg catgtgcggc cgactt
//
LOCUS       DAOQJH010000690         1616 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_70040_length_1616_cov_3.656630, whole genome shotgun sequence.
ACCESSION   DAOQJH010000690 DAOQJH010000000
VERSION     DAOQJH010000690.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1616
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70040_length_1616_cov_3.656630"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(198..854)
                     /locus_tag="PLQ18_13300"
     CDS             complement(198..854)
                     /locus_tag="PLQ18_13300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012700432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2076 domain-containing protein"
                     /protein_id="HNE02676.1"
                     /translation="MDQNEKSLIDDLYRRLQLAEQKTGPRDAQAEAVIREHFSRQPGV
                     AYYMTQAMLVQEHALKSLNERVQQLEQELASRPAAGGGSFLSGLFGGGASSAPAGRAG
                     IPPYQPQPGAFQQAPRGGFLSGALQTAAGVAGGVLLGHALMSMFSDVFGDSGAEAVAA
                     AVPEAAENFDLASAAEDIPSSGGFLGDAGSGFGGFGDSTASADNDFGGFGGDDDDQFV
                     "
     gene            1069..1608
                     /gene="sodC"
                     /locus_tag="PLQ18_13305"
     CDS             1069..1608
                     /gene="sodC"
                     /locus_tag="PLQ18_13305"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008460845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HNE02677.1"
                     /translation="MNKRLSLTLAAALAAAAGSAALAADSITISMDQLAADGGMTPAG
                     IVVATPAADGKGVVFKPELKGLPPGEHGFHVHEKPDCGPAEKDGKKVPGGNAGGHYDP
                     DKTGRHAGPTGDGHLGDLPALVVAADGTATAPVTAPRLKMSDLSGRSLMIHAGHDNYA
                     DQPQPLGGGGARMLCGVIR"
BASE COUNT          248 a          549 c          599 g          220 t
ORIGIN      
        1 ggctgtggcg gtgcaaggga tgggcaaggg gcggactagg ggtggtgcgg ccggaaagaa
       61 ccggccgccg tcgctgccgg gcgggagggt ggacggcaac ggcggcggcc ggggcaagac
      121 aagcggatga aacggatgaa gccggatgaa aaagcaggcg gcgccgcggt ggagggcgcc
      181 gccgggtggc gggacgctca gacgaactgg tcgtcgtcgt cgccgccgaa accgccgaaa
      241 tcgttgtcgg ccgaggcggt ggaatcgccg aagccgccga agccgctgcc ggcgtcgccg
      301 aggaagccgc cgctcgacgg gatgtcctcg gccgccgagg ccagatcgaa gttctcggcc
      361 gcttccggca cggccgcggc gaccgcttcg gcgccgctgt caccgaatac atcggagaac
      421 atgctcatca gcgcgtggcc gagcagcacg ccgccggcga cgccggccgc ggtctgcagc
      481 gcgccggaca ggaagccgcc gcgcggcgcc tgctggaagg cgcccggctg cggctggtag
      541 ggcggaatgc cggcacgacc ggccggcgcg ctgctcgcgc ccccgccgaa caggccggac
      601 aggaagctgc cgccgccggc ggccgggcgc gaggcgagtt cctgttcgag ctgctgcacg
      661 cgctcgttga ggctcttcag cgcgtgctcc tgcaccagca tcgcctgcgt catgtagtac
      721 gcgacgcccg gctggcggct gaagtgctca cggatcacgg cttcggcttg ggcatcgcgc
      781 gggccggtct tctgttcggc cagttgcagg cggcggtaca ggtcatcgat cagggatttt
      841 tcgttctggt ccatggttgg actccggtgc aggtgccgag gcaattttct atcaggtgat
      901 ttcttacctg taaagctcgc ccggctcggc ggtccggtac ggggtggcgc ttcagaccgc
      961 tcgccgggca ccgtgttcgc tggcaggagc gaaccgcacg ggcgcctaag ctgtccctaa
     1021 gccggcctgc ccccggctga ccatgaccac aagcaatgag gagtttccat gaacaagcga
     1081 ctgagtctga cgctggccgc cgccctggcc gctgcggccg gcagtgccgc actcgcggcc
     1141 gactcgatca cgatttcgat ggaccagctc gctgccgacg gcggcatgac gccggccggc
     1201 atcgtcgtcg ccacgccggc cgccgacggc aagggcgtgg tgttcaagcc cgaactgaag
     1261 ggcctgccgc ccggcgagca cggtttccac gtccatgaga agcccgactg cggcccggcc
     1321 gagaaggacg gcaagaaggt accgggcggc aatgccggcg ggcattacga tccggacaag
     1381 accggccggc atgccggccc gaccggcgac ggccacctcg gcgacctgcc ggcgctggtc
     1441 gtcgccgccg acggcacggc gaccgcgccg gtgacggcac cgcggctgaa gatgtcggac
     1501 ctgtccggcc gctcgctgat gatccacgcc ggtcatgaca actacgccga ccagccgcag
     1561 ccgctcggcg gcggcggcgc ccgcatgctc tgcggcgtga tccgctgacg gcgccg
//
LOCUS       DAOQJH010000691         1614 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_70222_length_1614_cov_3.723541, whole genome shotgun sequence.
ACCESSION   DAOQJH010000691 DAOQJH010000000
VERSION     DAOQJH010000691.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1614)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1614)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1614
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70222_length_1614_cov_3.723541"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            121..846
                     /locus_tag="PLQ18_13310"
     CDS             121..846
                     /locus_tag="PLQ18_13310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02678.1"
                     /translation="MSRQQYPRSSSHSPGARLINHWLSPAGGLVYHLRALRHRPLWRA
                     YSDRLAGWLQAWNPPCRELVLIGPSAGWTLPAAFLAGFGQVHVLEPDPLARRLLGRRF
                     ADAKLVFDRLDCLCDRDGPARLARAYPDAALLFANVLGQQVAGPVIADPGRLRRALAG
                     QHWASCHDVLSAARAPHGALPDTAAGLDAAALAEHLWGGSGLEVCDHDTLGLGGAPGP
                     LALWPLTPRAWHVIEWIVHAPAG"
     gene            complement(963..1313)
                     /locus_tag="PLQ18_13315"
     CDS             complement(963..1313)
                     /locus_tag="PLQ18_13315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018955155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HNE02679.1"
                     /translation="MDTPSEDPRARLERLASELDQRLARIGARERHEDGPPSQDFAEQ
                     ATERENDDVIGALALDTRLQLGLVRKALRRLDAGDYGVCVECGTPIPSARLAALPWAE
                     HCVACAERLEGRHA"
     gene            complement(1404..>1614)
                     /locus_tag="PLQ18_13320"
     CDS             complement(1404..>1614)
                     /locus_tag="PLQ18_13320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008931316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02680.1"
                     /translation="LLAGVLAAAGASALGWGLAQQVFDFPYRPDPLLWLAGAAAGVFG
                     VGLAGLAGTRAVLDQPPLATLRAGV"
BASE COUNT          236 a          575 c          573 g          230 t
ORIGIN      
        1 gtctgcttgg actgaaggaa agacgtaaat actgggcaaa agccaaaatg atactggccc
       61 agaaaaattc agcgaccgca gccaacccga caaacaacct gaatgcatgc attaaaagcg
      121 atgtcacgcc agcaataccc aagaagtagc tctcactcgc ccggtgctcg cctgatcaat
      181 cactggctgt ccccggccgg cggcctcgtc tatcacctgc gcgcgctgcg tcaccggccg
      241 ctctggcgag cgtattccga ccggctcgcc ggctggctgc aggcatggaa tccgccgtgc
      301 cgcgaactgg tgctgatcgg cccgtcggcg ggctggacgc tgccggcggc gtttctggcc
      361 ggcttcgggc aggtgcatgt gctggagccc gatccgctgg cgcgccggct gctcggccgc
      421 cggtttgcgg acgcgaaact cgtgttcgac cggctcgact gcctctgtga ccgggacggc
      481 ccggcgcggc tggcgcgggc ctaccccgat gccgcgctgc tgtttgcgaa cgtgctcggc
      541 cagcaggtcg cggggccggt gatcgccgat cccggccggc ttcgccgggc actggccgga
      601 cagcactggg cgagctgcca tgacgtgctg tcggcggcac gcgcaccgca cggcgcgctg
      661 cccgacacgg ctgccgggct ggacgccgcc gccctcgccg aacacctgtg gggcggcagc
      721 gggctggagg tctgcgacca tgacacgctc ggcctcggcg gcgcaccggg accgctggcg
      781 ctgtggccgc tgacgccgcg cgcctggcac gtcatcgaat ggatcgtgca tgcaccggcc
      841 ggctgatgcc gaccacacgt aaatgcatgg ttttcccctc tccccggccc ctctcccgcg
      901 aggggcgagg ggctgaaaag cgaagaagcg ttccggtgtg atcggtctgg gccgggcggg
      961 gctcaggcgt ggcggccttc cagacgctcc gcacaggcga cgcagtgctc ggcccagggc
     1021 agcgccgcga gccgggccga cgggatcggc gtgccgcatt cgacgcagac gccgtagtcg
     1081 ccggcatcga ggcgccgcag cgccttgcgc accagcccga gctgcaggcg cgtgtcgagc
     1141 gccagtgcgc cgatcacgtc atcgttctcg cgctcggtgg cctgttcggc gaaatcctgc
     1201 gacggcgggc cgtcctcgtg gcgttcgcgt gcgccgatgc gcgcgaggcg ctggtcgagt
     1261 tcgctggcga gccgttcgag gcgcgcacgc gggtcttcgc tcggagtgtc catcatcgtg
     1321 ctcccggtca ggggcgcagc cgcgagcacg cggccggcct gtccagagtc tggccgacga
     1381 tcgtctggtc gacgatcgcc gcgtcagaca ccggcgcgca gcgtcgcgag cggcggctga
     1441 tcgagcacgg cgcgcgtgcc ggcaagcccg gcaagcccga cgccgaacac gccggccgcc
     1501 gcaccggcga gccacagcag cggatcgggc cggtacggga aatcgaacac ctgctgcgcc
     1561 agtccccagc cgagcgcact ggccccggcc gccgccagca cgccggcgag cagg
//
LOCUS       DAOQJH010000692         1612 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_70445_length_1612_cov_2.393706, whole genome shotgun sequence.
ACCESSION   DAOQJH010000692 DAOQJH010000000
VERSION     DAOQJH010000692.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1612
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70445_length_1612_cov_2.393706"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..690)
                     /locus_tag="PLQ18_13325"
     CDS             complement(<1..690)
                     /locus_tag="PLQ18_13325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006490933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNE02681.1"
                     /translation="MHAEPIPSLAATADTQFIESRGRRLAYRSFGSGQPIVLCLRFRG
                     TMDEWDPLFLDTLAACGLRVVIFDYSGLGRSGGEPTYQPMSLAQDAVDLIEALDLHGV
                     AIGGWSIGGVAAQIVLTMFPQRIRHAVLIGTTPPGNLVKLAEPLFFETAGKEFNTAED
                     NVVLFFEPQSPTSRAESAASLQRMAARRDPGPKVDGLWAREWISGGPRNPVFPSEAVL
                     EGLKTTSIPMLH"
     gene            complement(817..1443)
                     /locus_tag="PLQ18_13330"
     CDS             complement(817..1443)
                     /locus_tag="PLQ18_13330"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017269767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HNE02682.1"
                     /translation="MNAAEPFVASETALLFVDPYNDFISEGGKFWERIREVAESRNLI
                     AHLQQIVSAARRAGVRLFYVPHRQWQPGDFEDWDHPTPYQLGAARFRMFEKGSWGGEW
                     RSEFAPQPGDVIAKEHYASSGFANTDLDFLLKQHRIRRVIVIGLLANTCIETTARFAT
                     ELGYHTTIVTDATAAVSEACMHAAHELNGPTYAHAILTTAEVVAALAG"
BASE COUNT          261 a          524 c          566 g          261 t
ORIGIN      
        1 gtgcagcatc gggatcgacg tggtcttcag cccttcgagc acggcctcgg acgggaagac
       61 cggattgcgc ggtccgccgc tgatccattc gcgtgcccac aggccgtcga ccttcggccc
      121 cggatcgcgg cgcgccgcca tgcgctgcag cgaggccgcc gattcggcgc gcgaggtcgg
      181 ggactgcggc tcgaagaaca gcacgacgtt gtcctcggcc gtgttgaatt ccttgccggc
      241 ggtctcgaag aacagcggct cggcgagctt cacgagattg ccgggcgggg tcgtgccgat
      301 cagcacggcg tggcggatgc gctgcgggaa catcgtcagc acgatctgcg ccgcaacgcc
      361 gccgatcgac cagccgccga tcgcgacgcc gtgcaggtcg agcgcctcga tcaggtcgac
      421 ggcatcctgc gccagcgaca tcggctgata ggtgggctcg ccgccggagc gccccagccc
      481 ggaataatcg aagatcacga cgcgcagccc gcaggcggcg agcgtgtcga ggaacagcgg
      541 atcccattcg tccatcgtgc cgcgaaagcg caggcacagc acgatcggct ggccgctgcc
      601 gaagctgcgg tacgcgagcc ggcggccccg gctttcgatg aactgcgtgt cggcggtggc
      661 tgccagactg gggatcggtt cggcatgcat ggtcgtcact ccgggctagc gggccgggcg
      721 gcccgaaatc gatgtgcgcc ggccggttcc gggcagcacg cggcctgccc cgcccgtgac
      781 gggcggggtt cggcagcttg ctgcagcgat gcggaatcag ccggccaggg cggcgaccac
      841 ctcggcggtc gtcaggatgg cgtgggcgta ggtcgggccg ttcagttcgt gcgcggcgtg
      901 catgcaggct tcgctgacgg cggccgtcgc atcggtgacg atcgtcgtgt gatagcccag
      961 ttcggtggcg aatcgggccg tggtttcgat gcaggtgttc gcgagcaggc cgatcacgat
     1021 cacccgccgg atgcggtgct gtttcagcag gaaatccaga tcggtattgg caaagccgct
     1081 cgatgcgtaa tgctccttcg cgatcacgtc accgggctgc ggtgcaaact cgctgcgcca
     1141 ttcaccgccc cagctgcctt tctcgaacat ccggaaacgg gcggcaccga gctgataggg
     1201 cgtcggatgg tcccagtcct cgaaatcgcc gggctgccac tgccggtgcg gcacgtaaaa
     1261 cagccggaca ccggcacggc gcgcggcact gacgatctgc tgcaggtgtg cgatcaggtt
     1321 gcgggattcg gccacctcgc ggatgcgctc ccagaacttg ccgccctccg aaatgaagtc
     1381 gttgtacgga tcgacgaaca gcagcgcggt ttccgatgcg acgaaaggtt ctgcggcatt
     1441 cacggcggtt cgctccttac ccggatcacc cggcggacag gtcagcgatg caggccgcag
     1501 agcatagtca ctatcacgat gatatccagt gacgacgctc acacggacac cggtggccgc
     1561 gcgtgcggcg cgactgcggg gcattccctc ttcccgcggc gggagatggg cc
//
LOCUS       DAOQJH010000693         1611 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_70487_length_1611_cov_4.413882, whole genome shotgun sequence.
ACCESSION   DAOQJH010000693 DAOQJH010000000
VERSION     DAOQJH010000693.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1611
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70487_length_1611_cov_4.413882"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(184..462)
                     /locus_tag="PLQ18_13335"
     CDS             complement(184..462)
                     /locus_tag="PLQ18_13335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013512020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02683.1"
                     /translation="MIAYESGLYVRIEEFPGPRAPLPRPLDSGFSPDVAYRVLGLFNP
                     SETSDAYFILSNDRDEIWFICNRHLRTTGIRSGGPLRLPLDCEMPAWS"
     gene            complement(764..1039)
                     /locus_tag="PLQ18_13340"
     CDS             complement(764..1039)
                     /locus_tag="PLQ18_13340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012974174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HNE02684.1"
                     /translation="MLRKHLLRPSLLSLLPAVAGHASHSLADVWNGLPRLGLRARLNG
                     FGHWLAAGYRRYRQRRALLALSDAMLKDIGISRADAWHEAGKSWWQD"
     gene            1160..>1611
                     /locus_tag="PLQ18_13345"
     CDS             1160..>1611
                     /locus_tag="PLQ18_13345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013715696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNE02685.1"
                     /translation="MPVNFDTDLLRTFVAIVDAGGFTQAAKQVHRTQSAVSMQVRRLE
                     EQVGKPLFQRDGRGVQLAPDGEALLGYARRLLKLHDEAVAALTQPELAGSVRLGTPDD
                     YVARFLPDILARYARCCPRVQIEVLCEPSVNLRRLLAEDRLDLALVTCL"
BASE COUNT          238 a          558 c          558 g          257 t
ORIGIN      
        1 tcatcgggct caccggcatt cgtcgactgc gcacgaccga cggcggatgc cgcgcgaccg
       61 gtgcagccgg acgcgtgatt ccacgattcc cggccccggc cggacaagcc cctgccacag
      121 gggtttgcga cttcttcggg gatatccgga tatatccggc agcagcgcgc gggcgccgcg
      181 ccctcaactc caggccggca tctcgcagtc gagcggcagg cgcagcggtc cgccgctgcg
      241 gatgccggtc gtgcgcagat gccggttgca gatgaaccag atctcgtcgc gatcgttgct
      301 caggatgaaa taggcatccg aggtctcgga cggattgaag aggccgagca cgcgatacgc
      361 gacatcgggg ctgaagccgc tgtccagcgg ccggggcagc ggcgcgcgcg ggccgggaaa
      421 ctcttcgatg cggacataaa gcccggattc ataggcgatc aagatggcgg cctcagcggg
      481 gggacggtgc cgcttcgacc atgcttttcc ggccgtgatt ccatgccgga accgccctcg
      541 ttcccggtct ttttcccggt ggcaccgggg ccgggcgtcg atctcccctc gcccatggcg
      601 gacgaggggc ttgttcgtca gcgccttgcc gtcgtgccgg tccatccgcg ctgtccgggc
      661 gggtccggga accgcggctg ccgcttgtgc agatacccat gccgtcccac cgagccttcc
      721 cgccgaggga acagcgtgaa aggcaggcgc cggccatgtt ccttcagtcc tgccaccagc
      781 tcttgccggc ctcgtgccag gcatcggcgc ggctgatgcc gatgtccttc agcatcgcgt
      841 cgctgagcgc gagcagcgcg cggcgctggc ggtagcgccg gtagccggcg gcgagccagt
      901 ggccgaagcc gttcagccgg gcacgcagtc cgaggcgcgg caggccgttc cagacatcgg
      961 cgaggctgtg cgaggcgtgg ccggcgacgg ccggcagcag cgacagcagg gacgggcgaa
     1021 gcaggtgttt gcggagcatg gcggctctcc ttcgtggtgt gaggccagca tggtccgcgc
     1081 tgattgattc atccaacgaa taggtttaat gactgccatc aaccatactg atgacactcg
     1141 ccctgccgga gccgccgtca tgcccgtgaa tttcgatacc gacctgctgc gcaccttcgt
     1201 cgccatcgtc gatgccggcg gcttcacgca ggccgcgaag caggtccacc gcacgcagtc
     1261 ggcggtgtcg atgcaggtgc gccggctgga ggaacaggtc ggcaagccgc tgttccagcg
     1321 cgacggccgc ggcgtgcagc tcgcgcccga cggcgaggcg ctgctcggct acgcgcgccg
     1381 gctgctgaag ctgcacgacg aggccgtcgc ggcgctgacg cagcccgaac tcgccggcag
     1441 cgtgcgcctc ggcacgcccg acgattacgt cgcccgcttc ctgcccgaca tcctcgcccg
     1501 ctacgcgcgc tgctgcccgc gcgtgcagat cgaggtgctc tgcgagccga gcgtcaacct
     1561 gcgccggctg ctggccgagg accgcctcga tctggcgctg gtcacctgcc t
//
LOCUS       DAOQJH010000694         1609 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_70636_length_1609_cov_5.303089, whole genome shotgun sequence.
ACCESSION   DAOQJH010000694 DAOQJH010000000
VERSION     DAOQJH010000694.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1609)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1609)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1609
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70636_length_1609_cov_5.303089"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            115..831
                     /locus_tag="PLQ18_13350"
     CDS             115..831
                     /locus_tag="PLQ18_13350"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HNE02686.1"
                     /translation="MKRLRIAVFGYHKLGIEVAKHLAPTPCELLVIDSSDDNLARAAE
                     RGHATLKCDYTDDLELARAGIGRDIDLIFCLLPDEAQNVFLIISARALDERLTIVAVS
                     DSPGSEQKLRAAGADKVIDPFDIAGRRISDLLVRPTVVDVLDQTVFGAADLEIAEVAV
                     TREGRLAGRRLLDASRLAGGTLVIVGLVRSGLERELRFALEAPEYVLTPGDVLMVVGP
                     AADVLAFRAEAAAGDAQRAI"
     gene            1120..>1609
                     /locus_tag="PLQ18_13355"
     CDS             1120..>1609
                     /locus_tag="PLQ18_13355"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE02687.1"
                     /translation="MNTETTHPPPPGVGIVDDDRELRTALRLLLESAGHVVETWADAD
                     ACLAAMHPQRSGCLLLDLRLPGMSGLSLQKALHERGIELPVILLSGHADTAIAVRAMH
                     EGAFDVLQKPCEPAVLLATVADAIAEDAARRQRRAPHERARQRLAQLSERERAVFDGI
                     VAG"
BASE COUNT          231 a          605 c          549 g          224 t
ORIGIN      
        1 gcccgcggcc ttcacgctgc aggccggcga ccagctcatc gtgctcgggc gcgaactgtc
       61 gatccagcac ctgcgcgccc aggtcgaagc cagcgccctc gcccggaggc cggcatgaag
      121 cgcctgcgca tcgccgtgtt cggctaccac aagctcggca tcgaggtcgc caagcacctc
      181 gcgccgacgc cgtgcgagct gctggtcatc gacagctcgg acgacaacct cgcgcgcgcc
      241 gccgagcgcg gccacgcgac gctcaagtgc gattacaccg atgacctcga actcgcgcgc
      301 gccggcatcg gccgcgacat cgacctgatc ttctgcctgc tgcccgacga ggcgcagaac
      361 gtgttcctga tcatctcggc gcgcgcgctc gatgagcgcc tgaccatcgt cgccgtgtcc
      421 gattcgccgg gatcggagca gaagctgcgt gccgccggcg ccgacaaggt catcgacccg
      481 ttcgacatcg ccggccgccg catctccgac ctgctggtgc gtccgaccgt cgtcgacgtg
      541 ctcgaccaga ccgtgttcgg cgccgccgat ctggagatcg ccgaggtcgc cgtgacccgc
      601 gagggccggc tggccgggcg gcgcctgctg gatgcgagcc gcctcgccgg cggcacgctg
      661 gtcatcgtcg gcctcgtgcg ctcaggtctg gagcgcgagc tgcgcttcgc gctggaagcg
      721 cccgagtacg tgctgacgcc cggcgacgtg ctgatggtgg tcggcccggc cgccgacgtg
      781 ctcgccttcc gcgccgaggc cgcagccggg gatgcccagc gcgcgatcta gccggctgcg
      841 cgccgggcct ccggcacggc ccccgcccgc ccggcgacga ccggatccgg cgcgcagccg
      901 tgcgaaaacc ctccgcagaa ttgatctggt gcaatgcttc cgaatccgat attcctgatc
      961 ctcgggcaca ggcgtcgcgg ggcaggccgg atatggcatg cagacgccgt gctccggatt
     1021 catccgactg acctggcatg acgggagaac atcggacccc ccgttccagc agcaccgggg
     1081 cggcgcagcg caacggaggc gatgcgcgcc gacaggcaca tgaacaccga gaccacgcat
     1141 ccgccgcctc cgggcgtcgg catcgtcgat gatgaccgcg agctgcgcac ggccctgcgc
     1201 ctgctgctcg aaagcgccgg ccatgtcgtc gagacctggg ccgacgccga tgcctgtctc
     1261 gccgccatgc atccgcagcg cagcggctgc ctgctgctcg acctgcgcct gcccggcatg
     1321 agcgggctga gcctgcagaa agccttgcac gagcgcggca tcgagctgcc cgtcatcctg
     1381 ctgagcggcc atgccgacac cgccatcgcc gtgcgcgcga tgcacgaggg cgccttcgac
     1441 gtgctgcaga agccgtgtga accggccgtg ctgctggcga cggtcgccga tgcgatcgcc
     1501 gaggatgccg cccggcgcca gcggcgtgcg ccgcacgagc gcgcgcgcca gcggctcgcg
     1561 cagctcagtg aacgcgagcg ggccgtgttc gacggcatcg tcgccggcc
//
LOCUS       DAOQJH010000695         1596 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_71729_length_1596_cov_5.467229, whole genome shotgun sequence.
ACCESSION   DAOQJH010000695 DAOQJH010000000
VERSION     DAOQJH010000695.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1596)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1596)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1596
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71729_length_1596_cov_5.467229"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..756)
                     /locus_tag="PLQ18_13360"
     CDS             complement(<1..756)
                     /locus_tag="PLQ18_13360"
                     /inference="COORDINATES: protein motif:HMM:NF013694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium and cobalt transport protein CorA"
                     /protein_id="HNE02688.1"
                     /translation="MTPAKEECAGIVICAAYADGRRIGDIKLDDIPQMLRRDGAFVWL
                     GLYEPDEALMQRVQQCFDLHDLAVEDAHKAHQRPKMEAYGDSLFIVLRTARWDDSAET
                     AAYGETHVFLGHNYLVSVRHGASQTYRPARLASERQPQLLAHGPSFALYALMDFVVDN
                     YFPVLSAYEDRLDELEADIFSGGADRDSTEEIYRLKRELVTLRRASLPLVDVCSDLMS
                     LRVALVPDSIRPYFRDVLDHLKRINDTLDTMREM"
     gene            859..>1596
                     /gene="pyrC"
                     /locus_tag="PLQ18_13365"
     CDS             859..>1596
                     /gene="pyrC"
                     /locus_tag="PLQ18_13365"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HNE02689.1"
                     /translation="MRLTLTRPDDWHLHVRDGAALATVVPHTARQFARAIIMPNLKPP
                     VTTTGQALAYRERILAAVPAGLDFEPLMTLYLTDRLDPAEIARARASGCIVACKLYPA
                     GATTNSDAGVTDIRLIHPVLAEMEKHGLLLLVHGEVTDADIDLFDREAVFIDRVLAPL
                     LRDFPGLKVVMEHITTREAVDFVAGAGPHLAATLTAHHLLYNRNAIFQGGIRPHYYCL
                     PVLKRETHRQALLAAATSGNPKFFLGTD"
BASE COUNT          250 a          572 c          512 g          262 t
ORIGIN      
        1 catttcgcgc atcgtgtcga gcgtgtcgtt gatgcgcttg aggtgatcga gcacgtcgcg
       61 gaagtacggg cggatcgagt ccggcaccag cgccacacgc agcgacatca ggtccgaaca
      121 gacatcgacc agcggcagcg acgcgcgccg caaggtcacg agttcgcgct tgaggcggta
      181 gatctcctcg gtcgagtcgc ggtccgcccc gcccgagaag atgtcggcct ccagctcgtc
      241 gagccggtcc tcgtaggccg agagcaccgg gaagtagttg tcgacgacga aatccatcag
      301 tgcgtacagc gcgaagctcg gtccgtgggc gagcagctgc ggctggcgtt cgctcgccag
      361 ccgcgccggc cggtaggtct gcgaggcgcc gtggcgcacc gagaccagat agttgtggcc
      421 gaggaacacg tgcgtctcgc cataggcggc agtctcggcc gagtcatccc agcgcgcggt
      481 gcgcagcacg atgaacagcg aatccccgta cgcctccatc ttcggacgct ggtgggcctt
      541 gtgcgcatcc tcgaccgcca gatcatgcag gtcgaagcac tgctgcacgc gctgcatcag
      601 ggcttcgtcc ggctcgtaga ggccgagcca gacgaaggca ccgtcgcggc gcagcatctg
      661 cgggatgtcg tcgagcttga tgtcgccgat gcggcggcca tcggcatagg ccgcgcagat
      721 gacgatgccg gcacattcct ctttcgctgg tgtcatgttc gtccccccgt ccgccggccg
      781 ggcgtcggcg cccgtgcctt gagtgctcgt tatactgccc tgctgctgat cgttccgttc
      841 cccgccggag tgctgccgat gcgcctgacc ctgacccgcc ccgatgactg gcatctgcac
      901 gtgcgcgacg gcgccgcact cgcgaccgtc gtgccgcaca cggcacgcca gttcgcgcgg
      961 gcgatcatca tgccgaacct gaagccgccg gtgacgacga ccggacaggc gctcgcctac
     1021 cgcgagcgca tcctcgccgc ggtgccggcc ggactcgatt tcgagccgct gatgacgctg
     1081 tacctgaccg atcgcctcga tccggccgag atcgccaggg cgcgcgcctc gggttgcatc
     1141 gtcgcctgca agctgtatcc ggccggcgcg acgacgaatt ccgatgccgg cgtcaccgac
     1201 atccggctga tccacccggt gctcgccgag atggaaaagc acggcctgct gctgctcgtg
     1261 cacggcgagg tcaccgatgc cgacatcgac ctgttcgatc gcgaggcggt gttcatcgac
     1321 cgcgtgctgg cgccgctgct gcgggacttc ccgggcctga aggtggtcat ggaacacatc
     1381 acgacccgcg aggccgtcga cttcgtcgcc ggcgccggtc cgcacctggc cgcgaccctc
     1441 accgcgcacc acctgctcta caaccgcaac gccattttcc agggcggcat ccgcccgcat
     1501 tactactgcc tgccggtgct caagcgcgaa acccaccgcc aggccctgct cgccgccgcg
     1561 acctccggca acccgaagtt cttcctcggc accgac
//
LOCUS       DAOQJH010000696         1595 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_71851_length_1595_cov_2.842857, whole genome shotgun sequence.
ACCESSION   DAOQJH010000696 DAOQJH010000000
VERSION     DAOQJH010000696.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1595)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1595)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1595
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71851_length_1595_cov_2.842857"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1527
                     /locus_tag="PLQ18_13370"
     CDS             <1..1527
                     /locus_tag="PLQ18_13370"
                     /inference="COORDINATES: protein motif:HMM:TIGR00361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HNE02690.1"
                     /translation="VAVRALWARLPGLAARLAPVRAGALAALLSALGYALIAGMGLPV
                     RRALIMVAVGSLALLAGRELLSTRTLALALIAVLLGDPLAPLSYGFWLSFGAVALILY
                     LHAGRLDGRGPTALALRLQWAISLGMLPATIVLFGQLPLVSPLANLLAIPWADLLIVP
                     FALAGLLASLASDTLAGPLLDLAGLTTRLLLGALDTLARPDWAARVVPTPPLWTLALA
                     LPGVAWWLAPPGFPSRRLGLLLCLPLLAWDWPHPPAGGFRLASLDAGSGLAVVVETAG
                     HTLLFDAGPRLGSTDAGRLVVLPYLQSRGLRRVDALVLTHLDASHIGGARSVRAALPV
                     GAIFVPDPLAMPAEGAASCVAGGFWIWDDVRFEWLHPPPGHGAGEARSCVLKVSGAGG
                     SALLIADLDAGGSRRLLAGGARLAADTLLAPYQGTKPPPAGLLAAVGPGRIWLSTGRG
                     NRWGHPKPEVLAAWAASGVQPDDTAAAGALLLDVPANATEPPLLTRWRETDRRYWRVH
                     "
BASE COUNT          140 a          608 c          599 g          248 t
ORIGIN      
        1 gtcgcggtgc gtgcgctctg ggcacggctg ccggggctcg cggcgcggct ggcgccggtg
       61 cgggccggtg cgctggcggc gctgctgagc gcgctcggct atgcgctgat cgccggcatg
      121 ggcctgccgg tgcgccgggc actgatcatg gtcgcggtcg gcagcctcgc gctgctcgcc
      181 gggcgcgagc tgctgtcgac gcgcacgctg gcgctggcgc tcatcgccgt gctgctcggc
      241 gatccgctgg cgccgctgtc ctacggcttc tggctgtcct tcggcgcggt ggcactgatc
      301 ctgtacctgc acgccggccg tctggacggg cgcgggccga cggcactggc gctgcgcctg
      361 cagtgggcga tcagcctcgg catgctgccg gcgaccatcg tgctgttcgg acagctgccg
      421 ctggtgtcgc cgctcgcgaa cctgctggcg atcccgtggg cggatctgct gatcgtgccg
      481 ttcgcgctgg ccgggctgct cgcgagtctg gcgtccgaca cgctggccgg accgctgctc
      541 gatcttgccg gactcacgac ccggctgctg ctcggcgcgc tcgacaccct cgcccgcccc
      601 gactgggcgg cgcgcgtcgt gccgaccccg ccgctgtgga cacttgcgct ggcgctgccc
      661 ggcgtcgcgt ggtggctggc gccgccgggc tttccgtcgc gccggctcgg gctgctgctc
      721 tgcctgccgc tgctcgcctg ggactggccg catccgccgg ccggcggctt ccggctcgcc
      781 tcgctcgatg ccggcagcgg cctcgccgtc gtcgtcgaga ccgccggcca cacgctgctg
      841 tttgacgccg gcccgcggct cggctcgacc gatgccggcc ggctcgtcgt gctgccctac
      901 ctgcagtccc gcggcctccg ccgggtcgat gcgctggtgc tgacacacct cgacgccagc
      961 cacatcggcg gtgcgcgcag cgtgcgcgcc gcgctgccgg tcggcgcgat cttcgtgccc
     1021 gacccgctgg cgatgccggc cgagggcgcc gcaagctgcg tggccggcgg cttctggatc
     1081 tgggatgacg tgcgcttcga atggctgcat ccgccgcccg gtcacggtgc cggcgaggcc
     1141 cgcagctgcg tgctgaaggt cagcggcgcc ggcggttcgg cgctgctgat cgccgatctc
     1201 gatgccggcg gcagccgccg gctgctggcc ggcggcgccc ggcttgcggc cgatacgctg
     1261 ctcgccccgt atcagggcac gaagccaccc ccggccggcc tgctggccgc ggtcgggccg
     1321 ggacgcatct ggctgtccac gggccgcggc aaccgctggg gacatccgaa acccgaagtg
     1381 ctcgcggcct gggcggcctc cggcgtgcag cccgacgaca ccgccgcggc cggagcgctg
     1441 ctgctcgacg tgccggcgaa cgccacggag ccgccgctcc tcacgcgctg gcgcgaaacc
     1501 gatcgccgct actggcgggt gcactgaagc ctgccgtctg cgcccgcgcg gtgcagaccg
     1561 tcgcaccggc gggccagaac gggccaaggt ggtgt
//
LOCUS       DAOQJH010000697         1590 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_72272_length_1590_cov_3.921824, whole genome shotgun sequence.
ACCESSION   DAOQJH010000697 DAOQJH010000000
VERSION     DAOQJH010000697.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1590)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1590)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1590
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72272_length_1590_cov_3.921824"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..891)
                     /locus_tag="PLQ18_13375"
     CDS             complement(<1..891)
                     /locus_tag="PLQ18_13375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013159220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HNE02691.1"
                     /translation="MSPELPITAVLPALAQTLAAAPAVVLQAPPGAGKSTGVPLALLD
                     APWLAGRRIVMLEPRRLAARAVAARMSALLGEPPGATVGYRVRFEARVSARTRIEVVT
                     EGILTRRLQSDPELAGVGLLIFDEFHERSLHADLALALARDAQLGLREDLKLLLMSAT
                     LDGERLCRLLDDAPLVSSEGRAHPVAIRHVAREPEAPLPEALAQQVVRLLAEESGDVL
                     AFLPGGAEIRRAAERLRTLAPQVSVLPLYGEPPAAEQQRALEPAPDGRRRVVLATTIA
                     ETSLTIDGIRLVVDGGWTGAP"
     gene            1113..>1590
                     /locus_tag="PLQ18_13380"
     CDS             1113..>1590
                     /locus_tag="PLQ18_13380"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE02692.1"
                     /translation="MKTALVVDDSRLARLALTRALEKHGLHVEQVGSAAEAFEFLRGR
                     RPDVVFMDVTMPEMDGFTAAGLITRDPQFAGLPVVMCSAEETDDVRTRTAAAGAMAFL
                     GKTAGDAGLSDILNRLVPEAASMPAAAPALAAAGPSAAELAAQIESSVAAAVASRLV"
BASE COUNT          233 a          528 c          623 g          206 t
ORIGIN      
        1 cggcgcgcct gtccagccgc cgtcgacgac gaggcggatg ccgtcgatgg tcaggctggt
       61 ctcggcgatc gtggtcgcga gcaccacgcg ccggcggccg tcgggagcgg gttccagcgc
      121 acgctgctgc tcggcagccg gcggctcgcc gtagagcggc agcacgctga cctgcggcgc
      181 cagcgtgcgc aggcgctcgg cggcgcggcg gatctcggcg ccaccgggca ggaaggcgag
      241 cacgtcgccg gattcctcgg cgagcagccg cacgacctgc tgcgccagcg cctcgggcag
      301 cggggcttcg ggttcgcgcg cgacgtggcg gatcgcgacc ggatgcgcgc ggccttcgct
      361 gctcacgagc ggcgcatcgt cgagcagccg gcagagccgc tcgccgtcga gcgtggccga
      421 catcagcagg agcttcaggt cctcgcgcag gccgagctgc gcgtcgcgcg ccagcgccag
      481 cgccagatcg gcgtgcaggc tgcgctcgtg aaactcgtcg aagatcagca ggccgacgcc
      541 ggcaagctcc ggatcggact gcagccgccg ggtcaggatg ccttcggtga cgacctcgat
      601 gcgcgtgcgc gccgagaccc gggcctcgaa gcgcacgcgg tagccgaccg tggcgccggg
      661 cggttcgccg agcagcgcgc tcatgcgcgc cgcgaccgcg cgggcggcca ggcggcgcgg
      721 ttcgagcatc acgatgcgcc ggcccgcgag ccacggcgca tcgagcagcg cgagcggcac
      781 gccggtgctc ttgccggcgc cgggcggggc ctgcagcacg acggccgggg ccgcggcgag
      841 ggtctgtgcc agagccggca gcacggccgt gatcggcagt tcaggggaca tggtgcttac
      901 aaagcccttg caaaagacgt gtccaggtgg caggcggctg gggcgtggag tgtaaaacat
      961 cgtcaaacag gggcgcggtg aaaatcccgg acatgggaaa agtggctcgc tatggttcta
     1021 atcgcccggc cacagggagc cccgcaccgg agtgcggaag ggcggcaggg aacatgattc
     1081 ggatcgggac tggatcggtg aggaaatcga cgatgaagac agcgttggtc gtggacgatt
     1141 cgcgactcgc caggctggcg ttgacgcgcg ccctcgagaa gcacgggctg cacgtcgaac
     1201 aggtcggctc ggccgcggaa gccttcgagt ttctgcgggg ccgccggccg gatgtggtgt
     1261 tcatggacgt gacgatgccg gagatggacg gcttcacggc cgccggcctg atcacgcgcg
     1321 atccccagtt cgccgggctg ccggtggtga tgtgctcggc cgaggaaacc gacgacgtgc
     1381 gcacccgcac cgctgcagcc ggcgccatgg ccttcctcgg caagacggcc ggcgatgccg
     1441 ggctgtcgga catcctgaac cgtctggtgc cggaggcggc gtcgatgccg gcggctgcgc
     1501 cggcgctggc ggcggccgga ccgagcgcgg cggaactcgc cgcgcagatc gaatcctcgg
     1561 tggcggcggc ggtcgcctcg cggctggtgg
//
LOCUS       DAOQJH010000698         1577 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_73407_length_1577_cov_4.515769, whole genome shotgun sequence.
ACCESSION   DAOQJH010000698 DAOQJH010000000
VERSION     DAOQJH010000698.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1577)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1577)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1577
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73407_length_1577_cov_4.515769"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(167..>1577)
                     /locus_tag="PLQ18_13385"
     CDS             complement(167..>1577)
                     /locus_tag="PLQ18_13385"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNE02693.1"
                     /translation="AAEPLRGAVLVCQDVTARRAFQRELHHQAAHDHLTGLLNRRAFE
                     RALDELVTALHRGPAREHALCFVDLDRFKAVNDTAGHAAGDALLGRVAALLRAGVRGT
                     DVIARLGGDEFALILVDCPQAQAVRVADKLVAAVRSLRFIWEGQVHEIGASIGLVPVT
                     AGSGNVATLTSQADVACYAAKGLGRNRVAVYSPDNEAIAREHREISAADGIRQALEAG
                     RFSLYAQPIVAVGTPESEQYFELLLRMVDGEGRLVPPGAFLPAAERYELMPALDRWAI
                     GHALHTLGPTIAALPALMVSLNLSAGSINDPSFRPWLEAALAQTPVPVARIGFEFAES
                     VAVSNLIAAGDLIQWLRARGCRVALDDFGTGLSSFSYLKHFPVDFVKIDGSFIAALGH
                     GAVDRAIVESLNDIAHRLGVRTIAEGVESSETLGLLQQLGIDYAQGMALGAPVALGEQ
                     LAALRELDGGGRPKLKLVR"
BASE COUNT          245 a          568 c          562 g          202 t
ORIGIN      
        1 atcaacgccg cgtacttcac ctaccacgag aaggaaacgc cgccggccgc cgcctgaagc
       61 gcagcgcagc gccgacgcac gcgaggccgg tgccctccgg ggcaccggcc tctttcatct
      121 atatgagtgc cgaccgggcg cgccggcagg ccgggcgccg gccggctcag cgcaccagct
      181 tcagcttcgg ccggccgccg ccgtcgagtt cgcgcagggc ggcgagctgc tcgccgagcg
      241 ccaccggcgc accgagcgcc atgccctgcg cgtaatcgat gccgagctgc tgcagcagac
      301 ccagcgtctc ggacgactcg acgccttcgg cgatcgtgcg cacgccgagc cggtgcgcga
      361 tgtcgttcag cgattcgacg atggcgcgat cgaccgcacc gtggccgagc gcggcgatga
      421 agctgccgtc gatcttcacg aaatcgaccg ggaagtgctt gagatagctg aaggaactga
      481 ggccggtgcc gaaatcgtcg agcgccaccc ggcagccgcg ggcgcgcagc cactggatca
      541 ggtcgccggc ggcgatcagg ttgctgacgg cgaccgactc ggcgaactcg aacccgatgc
      601 gtgccaccgg caccggcgtc tgcgccagcg ccgcctccag ccagggccgg aacgacggat
      661 cgttgatcga gccggccgac aggttcagcg acaccatgag cgcgggcagg gcggcgatgg
      721 tcggcccgag cgtgtgcagc gcgtgaccga tcgcccagcg atccagcgcc ggcatcagct
      781 cgtagcgttc ggcggccggc aggaaggccc cgggcggcac cagccggccc tcaccgtcga
      841 ccatgcgcag cagcagctcg aaatactgtt cggattccgg cgtgccgacg gcgacgatcg
      901 gctgcgcgta caggctgaag cgcccggcct ccagcgcctg gcggatgccg tccgctgccg
      961 agatctcgcg atgctcgcgg gcaatggcct cgttgtccgg ggaatagacg gcgacgcggt
     1021 tgcgcccgag gcccttggcc gcatagcagg ccacatcggc ctggctggtg agggtggcga
     1081 cgttgccgga gccggccgtg accggcacca gaccgatgct cgcgccgatc tcgtgcacct
     1141 gcccttccca gatgaagcgc aggctgcgca cggcggcaac cagcttgtcg gcgaccctga
     1201 ccgcctgcgc ctgcgggcag tcgacgagga tcagcgcgaa ctcgtcgccg cccagacgtg
     1261 cgatcacgtc ggtgccgcgc accccggcgc gcagcagcgc cgcgacccgc ccgagcagcg
     1321 catcgccggc cgcgtggccg gcggtgtcgt tgacggcctt gaagcggtcg agatcgacga
     1381 agcagagcgc gtgctcgcgc gccggcccgc ggtgcagcgc cgtgaccagc tcatcgagcg
     1441 cgcgttcgaa ggcacgccgg ttcagcaggc cggtcaggtg atcgtgggct gcctgatggt
     1501 gcagctcgcg ctggaaggcc cggcgggcgg tcacgtcctg acagaccagc accgcgccgc
     1561 gcaacggctc ggctgcc
//
LOCUS       DAOQJH010000699         1576 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_73531_length_1576_cov_3.716634, whole genome shotgun sequence.
ACCESSION   DAOQJH010000699 DAOQJH010000000
VERSION     DAOQJH010000699.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1576
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73531_length_1576_cov_3.716634"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(218..412)
                     /locus_tag="PLQ18_13390"
     CDS             complement(218..412)
                     /locus_tag="PLQ18_13390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HNE02694.1"
                     /translation="MTKNVGQIDKILRLLVGLVLIALTLSGQIGVWGWIGVLPIATAL
                     MNWCPAYTLLGVRTCPVEPK"
     gene            711..1025
                     /locus_tag="PLQ18_13395"
     CDS             711..1025
                     /locus_tag="PLQ18_13395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013420113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNE02695.1"
                     /translation="MDRSANAAAQLLKALASPVRLKILCQLVDGEKAVGQLADLLQAR
                     DALVSQHLALLRKDGLVRTRREAQTIWYSIASPAAERIIRTLYELYCAGDGAGPAPGP
                     KP"
     gene            1052..>1576
                     /locus_tag="PLQ18_13400"
     CDS             1052..>1576
                     /locus_tag="PLQ18_13400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese family protein"
                     /protein_id="HNE02696.1"
                     /translation="MSLTRLDARSLQVRLAEDRADLYDIREPAEYAREHIDGARLLPA
                     GRLGRELPDRTPGRLAVFMCQSGMRTTENATRILGCISGEVAMLDGGLEAWKRAGLPV
                     IEDRSAPLPLMRQVQIVAGSLVLLGVVLGFLLGPAWFGLAGFVGAGLVFAGLTGFCGM
                     ARLLAQLPWNRIDPP"
BASE COUNT          225 a          534 c          548 g          269 t
ORIGIN      
        1 tgtgcgccag ctcgtcgatc acgctttcga ccgcgaaagc ggccatcggc gccggccacg
       61 gtcgccagcg ctttcaggtc gcgctgctgc tggcgctgtg catggccggt ctggtggtcg
      121 cgctcgggct gtatgccggc tgaagcgcgc tgatccgccc ggatgaaaaa agccccgctg
      181 atgcggggct tttcgtttgt gccggcgggc gcgcgactta cttcggctcg accgggcagg
      241 tgcgcacgcc gagcagcgtg taggccgggc accagttcat cagcgcggtg gcgatcggca
      301 gcacgccgat ccagccccag acgccgatct ggccgctcag tgtcagggcg atcagcacga
      361 ggccgacgag caggcgcagg atcttgtcga tctgtccgac gttcttggtc atgacgaatg
      421 tcctccggga tggtggatgc cgggtaggcg tgacggctct gtgttttccg ctctttccga
      481 accctttccc ccaaggggag gggctcggga agacggtgtg ttccgccggg acgggtatga
      541 aagacctgcc cgcgaccgat gcccggcatc ggcgcgcgag acgttccgct aggctggcag
      601 acccgtctac accgcggcgg cgggtgacat tcccttgcca tgcatcgggt ttccgctgtt
      661 tgacagcata atcatgtatt cgtatattcg caaatatgaa cattgacgag atggaccgct
      721 ccgcgaacgc cgcggcgcag ttgctgaagg ccctcgccag tccggtgcgc ctgaagatcc
      781 tctgccagct cgtcgatggc gagaaagccg tcggccagct cgccgatctg ctgcaggccc
      841 gcgacgcgct ggtttcgcag catctggcgc tgctgcgcaa ggacgggctg gtgcgcacgc
      901 ggcgcgaggc acagacgatc tggtactcga tcgcgagccc ggcggccgaa cgcatcatcc
      961 gcacgctcta cgagctgtac tgcgccggcg acggcgcggg accggctccc ggacccaagc
     1021 cgtaaccgac tgactgacgg agacctgccc catgagcctg acccgcctcg atgcccgcag
     1081 cctgcaggtg cgcctcgccg aggaccgtgc cgacctgtac gacatccgcg agccggccga
     1141 atacgcccgc gagcacatcg atggcgcgcg gctgctgccg gccggccggc tcggccgcga
     1201 gctgcccgat cgcacgccgg ggcggctcgc ggtgttcatg tgccagagcg gcatgcgcac
     1261 caccgagaac gccacgcgca tcctcggctg catcagcggg gaggtggcaa tgctcgacgg
     1321 cgggctggag gcctggaagc gcgccggcct gccggtgatc gaggaccgct cggcgccgct
     1381 gccgctgatg cgtcaggtgc agatcgtagc cggcagtctg gtgctgctcg gcgtcgtgct
     1441 cggcttcctg ctcggcccgg cctggttcgg gctggccggc ttcgtcggtg ccggcctcgt
     1501 gttcgccggc ctcaccggct tctgcggcat ggcgcggctg ctcgcgcagc tgccgtggaa
     1561 ccgcatcgat ccgccg
//
LOCUS       DAOQJH010000700         1568 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_74297_length_1568_cov_4.668870, whole genome shotgun sequence.
ACCESSION   DAOQJH010000700 DAOQJH010000000
VERSION     DAOQJH010000700.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1568
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74297_length_1568_cov_4.668870"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..380)
                     /locus_tag="PLQ18_13405"
     CDS             complement(<1..380)
                     /locus_tag="PLQ18_13405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003302842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNE02697.1"
                     /translation="MARLEAREIAKRYKGRRVVDAASVHVDSGEVVGLLGPNGAGKTT
                     SFYMIVGLIGCDEGHVLLDSREITQLPMHARARLGVGYLPQEASVFRKMTVLDNVLAI
                     LETRGDLDRSARTERAETLLHELNV"
     gene            complement(384..971)
                     /gene="lptA"
                     /locus_tag="PLQ18_13410"
     CDS             complement(384..971)
                     /gene="lptA"
                     /locus_tag="PLQ18_13410"
                     /inference="COORDINATES: protein motif:HMM:TIGR03002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HNE02698.1"
                     /translation="MRRLPRNLIAPSRITRGRITSGLIAPGLLACALSAQALPEDRQQ
                     PVNLVADRAEMNQQTGVATYEGNVIVTQGTMRLTGDKVVVYTQEGELQRMESFGKPTT
                     FKQKPAADKEEILGESLKLEYDARTGIVIATGKAKITQVKDTFTGDRIEYDVNKDLVK
                     ARGGESAGRVQIILQPKPEGGTPVPGARPAQPKKP"
     gene            complement(937..>1568)
                     /gene="lptC"
                     /locus_tag="PLQ18_13415"
     CDS             complement(937..>1568)
                     /gene="lptC"
                     /locus_tag="PLQ18_13415"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HNE02699.1"
                     /translation="TCTTCCAQACSEGAIRRVRTLLVLVALALLAGLSGWFLNDLERD
                     LREVRARDDRLPLTIAENFVATVMNARGERLYVLTGPHLEQFPAPRGTFVTRPLAHTY
                     ENLAPVWEIRAEQGWIAPGTETIELRGDVHAERPASTGYVPVIMTGSNLRLIPDKNYA
                     ESAEPVRVQTPDGVIDAVGFHAWLDEDRIELLADVRGHYAPPAQKPDRP"
BASE COUNT          203 a          495 c          577 g          293 t
ORIGIN      
        1 acgttcagtt cgtgcaacag ggtctcggcg cgttcggtgc gcgcgctgcg gtcgaggtcg
       61 ccgcgggttt cgaggatggc gagcacgttg tcgagcacgg tcatcttgcg gaacaccgag
      121 gcttcctgcg gcaggtagcc gacgccgagg cgcgcgcgcg catgcatcgg cagctgcgtg
      181 atctcgcggc tgtcgagcag cacatgacct tcgtcgcagc cgatcaggcc gacgatcatg
      241 tagaagctcg tggtcttgcc ggcgccgttc ggcccgagca ggccgacgac ctcgccgctg
      301 tcgacgtgca cgctggcggc atcgacgacg cgccggccct tgtagcgctt ggcgatttcg
      361 cgcgcttcga ggcgggccat ggctcagggc ttcttcggct gcgcgggtct ggcgcccggg
      421 accggcgtgc cgccttcggg cttgggctgc aggatgatct gcacgcggcc ggcgctctcg
      481 ccgccgcggg ccttcacgag gtccttgttg acgtcgtact cgatgcggtc gccggtgaag
      541 gtgtccttga cctgcgtgat cttcgccttg ccggtggcga tgacgatgcc ggtcctggcg
      601 tcgtattcga gcttcagcga ctcgccgagg atttcctcct tgtcggcggc cggcttctgc
      661 ttgaacgtcg tcggcttgcc gaagctctcc atgcgctgca gttcgccctc ctgcgtgtag
      721 accacgacct tgtcaccggt caggcgcatc gtgccctgcg tgacgatgac gttgccctcg
      781 taggtggcga cgccggtctg ctggttcatc tcggcgcggt cggcgaccag gttgaccggt
      841 tgctggcggt cttcgggcag ggcctgggcg ctcagggcgc aggcgagcag gccgggggcg
      901 atcaggccgg acgtgatccg gccacgggtg atccggctag gggcgatcag gtttctgggc
      961 aggcggcgca tagtgtcctc ggacgtcggc aagcagttcg atgcgatcct cgtcgagcca
     1021 tgcgtggaag ccgacggcgt cgatgacgcc atccggggtc tgcacacgca cgggctcggc
     1081 ggattcggcg tagttcttgt cggggatcag ccgcaggttg ctgccggtca tgatcaccgg
     1141 cacgtaaccg gtgctggccg gacgctcggc atgcacgtcg ccgcgcagtt cgatggtttc
     1201 ggtgccggga gcgatccagc cctgttcggc gcggatttcc cagaccggcg ccagattctc
     1261 gtaggtgtgc gcgagcgggc gggtcacgaa ggtgccgcgc ggggccggaa actgttcgag
     1321 gtgcggaccg gtgagcacgt acagccgttc gccgcgggcg ttcatgaccg tggccacgaa
     1381 gttttcggcg atggtgagcg gcaggcggtc gtcgcgtgcg cgcacctcgc gcaggtcgcg
     1441 ttcgagatcg ttcaggaacc agccgctcag cccggcgagc agggccagcg cgacgaggac
     1501 gagcagcgtg cgcacgcgcc ggatggctcc ctcagagcac gcctgcgcgc agcaggtcgt
     1561 gcatgttc
//
LOCUS       DAOQJH010000701         1566 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_74493_length_1566_cov_3.456651, whole genome shotgun sequence.
ACCESSION   DAOQJH010000701 DAOQJH010000000
VERSION     DAOQJH010000701.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1566)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1566)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1566
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74493_length_1566_cov_3.456651"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1566
                     /locus_tag="PLQ18_13420"
     CDS             <1..>1566
                     /locus_tag="PLQ18_13420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HNE02700.1"
                     /translation="LIYDQTCAAEKRRRRKRGLMPDPPRRVFINAAVCEGCGDCSVQS
                     NCLSVVPLETGFGRKRAIDQSNCNKDYSCLNGFCPSFVTVHGAKLRGNRTALAADDWS
                     LALPEPGLPALTEPWGIVITGVGGTGVVTIGALLGMAAHLEGKGVSVLDMTGLAQKYG
                     AVVSHVRIAAQPEAIHAVRIAAGGARLLLGCDYVVAAGSEALSKLDPQHSHAIINAHQ
                     AMPARFVREPDLNFPDEAMRTAIREAAGDAEFIDATALATGLFGDALASNLFLLGFAW
                     QRGRVPLSGEAILRAIELNGAAAEANRAAFLWGRRAAIDEAAVRRRALPAAVSELKRP
                     PALDEWIGQRAAFLGEYQDADWAARYRDLVARVRSAEAGLGSETLTRTVAQVLFRLMS
                     YKDEYEVARLYVDAGFRASLDAVFEPGYTLGFHLAPPLFAKRDPHTGLPVKQTFGPWL
                     MRVFGWLAQGRRLRGTWLDPFGWTAERRGERRLIGEYEQLVDELLGGLSRERLTLAVE
                     IAGLHGQVRGFGH"
BASE COUNT          214 a          558 c          567 g          227 t
ORIGIN      
        1 tgctgatcta cgaccagacc tgcgcggccg agaagcgccg ccggcgcaag cgcgggctga
       61 tgccggatcc gccgcggcgg gtgttcatca acgccgcggt ctgcgagggc tgcggggatt
      121 gcagcgtgca gtcgaactgc ctgtcggtcg tgccgctcga gaccggattc ggtcgcaagc
      181 gcgcgatcga ccagtcgaac tgcaacaagg actactcctg cctgaacggc ttctgcccga
      241 gcttcgtgac cgtgcacggc gcgaagctcc gcggcaaccg cacggcgctg gcggccgatg
      301 actggtcgct ggcgctgccc gaacccggcc tgccggcgct caccgagccc tggggcatcg
      361 tcatcaccgg cgtcggcggc accggtgtcg tgaccatcgg cgcgctgctc ggcatggccg
      421 cgcacctcga aggcaagggt gtgagcgtgc tcgacatgac cgggctcgcg cagaagtacg
      481 gcgccgtggt ctcgcacgta cgcatcgccg cgcagcccga agccatccac gccgtgcgca
      541 tcgccgccgg cggcgcccgg ctgctgctcg gctgcgacta cgtcgtcgcc gccggcagcg
      601 aggcgctctc caaactcgat ccgcagcact cgcacgcgat catcaatgcc catcaggcga
      661 tgccggcgcg cttcgtgcgc gagccggacc tgaacttccc ggacgaggcc atgcgcacgg
      721 cgatccgcga ggccgccggc gatgccgagt tcatcgatgc gaccgcgctc gccaccggcc
      781 tgttcggcga tgcgctggcg agcaacctgt tcctgctcgg cttcgcctgg cagcgcgggc
      841 gcgtgccgct gtcgggggag gcgatcctgc gcgcgatcga gctcaacggc gcggcggccg
      901 aggccaaccg ggccgcgttc ctgtgggggc ggcgcgccgc gatcgacgag gctgcggtgc
      961 gccggcgcgc gctgccggcg gcggtcagcg agttgaagcg cccgccggcg ctcgacgagt
     1021 ggatcggaca gcgtgcggcc tttctcggcg aataccagga tgcggactgg gccgcgcgct
     1081 accgcgacct cgtcgctcgc gtgcgcagcg ccgaagccgg cctcggcagc gagacgctga
     1141 cgaggaccgt cgcgcaggtg ctgttccggc tcatgagtta caaggacgaa tacgaggtcg
     1201 cgcgcctgta cgtcgatgcc ggcttccgcg ccagcctcga tgccgtgttc gagcccggct
     1261 acacgctcgg tttccacctc gcgccaccgc tgttcgcgaa gcgcgatccg cacaccggcc
     1321 tgccggtgaa gcagaccttc gggccgtggc tgatgcgcgt gttcggctgg ctcgcgcagg
     1381 gccggaggct gcgcggcacc tggctcgatc ccttcggctg gaccgccgaa cggcgcggcg
     1441 agcgccggct gatcggcgag tacgaacagc tcgtcgacga actgctgggc gggctgagcc
     1501 gggagcgcct gacgctcgcc gtcgagatcg ccgggctgca cggtcaggtc cggggcttcg
     1561 gccacg
//
LOCUS       DAOQJH010000702         1566 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_74496_length_1566_cov_3.338848, whole genome shotgun sequence.
ACCESSION   DAOQJH010000702 DAOQJH010000000
VERSION     DAOQJH010000702.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1566)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1566)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1566
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74496_length_1566_cov_3.338848"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="PLQ18_13425"
     CDS             <1..447
                     /locus_tag="PLQ18_13425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02701.1"
                     /translation="LAAAGTGLGIALPKLRAWLYGDAPAIDLTRDDTACRPIGVPCSA
                     THARLQLALGLGQSVSPLQAFPVTVSVQGAEAGGLRSVELAFTMQGMDMGILRTPLLR
                     QADGSWRGRGLLPVCAAGRSDWLATVTATGADGERYRAQFAFPAGH"
     gene            508..>1566
                     /locus_tag="PLQ18_13430"
     CDS             508..>1566
                     /locus_tag="PLQ18_13430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNE02702.1"
                     /translation="MRILAIRGRNLASLAGDFAVELAAPPLAQAGLFAITGPTGAGKS
                     TLLDALCLALYDEMPRLAGAPKVAVVPGADADERLNADDVRGILRRGCTEACAEVDFL
                     GHDRRRYRARWELRRARGKADGRTQNQTLVLTDLDTQQRLGGTKTETLAEIRARLGLS
                     FEQFRRSALLAQGDFASFLRARAQERAELLERITGTQLYGELSIAAHRRARDEEDALR
                     ELEQGLAALEPLDESARAELLAGHARQAEALAVLEARHLALRRLADWQTRSRTLAVEL
                     AEADRALQQARAAVDAAGLRQQVWQRIRAAQPLRPVVEAVERTRTAAEAASQRLAGAC
                     QALADADAGLRQAQVEAQA"
BASE COUNT          197 a          600 c          583 g          186 t
ORIGIN      
        1 ctggccgcgg ccggcacggg cctcggcatc gcgctgccga agctgcgcgc ctggctgtac
       61 ggcgacgctc cggccatcga tctgacccgc gatgacaccg cctgccggcc gatcggcgtg
      121 ccgtgctcgg cgacgcacgc gcgcctgcag ctcgcgctcg gcctcgggca gtcggtctcg
      181 ccgctgcagg cgtttccggt caccgtcagc gtgcagggtg ccgaggccgg cgggctgcgc
      241 tcggtcgagc tggcgttcac gatgcagggc atggacatgg gcatcctgcg cacgccgctg
      301 ctgcgtcagg ccgacggcag ctggcgcggg cgcgggctgc tgccggtgtg cgcggccggg
      361 cgcagcgact ggctcgcgac cgtgaccgcc accggcgccg atggcgagcg ctaccgcgcg
      421 cagttcgcgt tcccggccgg gcactgaacg caagcccggc accgtcatcc gcttcgccgt
      481 cacctcgccg ccacagggac agtccgcatg cgcatcctcg cgatccgggg ccgcaacctc
      541 gccagtctgg ccggcgactt cgccgtcgag ctcgccgcgc cgccgctggc gcaggccggg
      601 ctgttcgcga tcaccggccc gaccggggcc ggcaagtcga cgctgctcga tgcgctgtgc
      661 ctggcgctgt atgacgagat gccgcgcctc gccggtgcgc cgaaggtcgc ggtcgtgccc
      721 ggcgcggatg ccgatgagcg gctcaacgcc gatgacgtgc gcggcatcct gcgccgcggc
      781 tgcaccgagg cctgcgccga ggtcgatttc ctcggccacg accgccggcg ctaccgcgcg
      841 cgctgggagc tgcgccgcgc ccgcggcaag gccgatggcc gcacccagaa ccagacgctg
      901 gtgctcaccg atctcgacac gcagcagcgc ctcggcggca cgaagaccga gacgctggcc
      961 gaaatccgcg cccggctcgg cctgagcttc gagcagttcc ggcgcagcgc gctgctcgcg
     1021 cagggtgact tcgcgagctt cctgcgcgcc cgggcccagg agcgcgccga gctgctggag
     1081 cgcatcaccg gcacgcagct ctacggcgaa ctgtcgatcg ccgcgcaccg ccgcgcgcgc
     1141 gacgaggaag acgcgctgcg cgaactggaa caaggcctcg ccgcgctcga accgctggac
     1201 gagtccgcgc gggccgaact gctcgccggg cacgcacggc aggccgaagc gctggccgtg
     1261 ctcgaagccc ggcacctcgc gctgcgccgc ctcgccgact ggcagacgcg cagccgcacg
     1321 ctcgccgtcg aactcgccga agcggatcgg gcgctgcagc aggcccgggc cgccgtcgat
     1381 gcggccgggc tgcggcagca ggtctggcag cggatccggg ccgcccagcc gctgcgcccg
     1441 gtggtcgagg ccgtcgagcg cacgcgcacc gcggccgaag cggcgagcca gcggcttgcc
     1501 ggggcgtgcc aggcgctggc ggacgccgat gccgggctgc ggcaggcgca ggtagaagcg
     1561 caggcc
//
LOCUS       DAOQJH010000703         1565 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_74577_length_1565_cov_4.103974, whole genome shotgun sequence.
ACCESSION   DAOQJH010000703 DAOQJH010000000
VERSION     DAOQJH010000703.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1565)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1565)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1565
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74577_length_1565_cov_4.103974"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /gene="rph"
                     /locus_tag="PLQ18_13435"
     CDS             <1..235
                     /gene="rph"
                     /locus_tag="PLQ18_13435"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012639495.1"
                     /note="RNase PH; tRNA nucleotidyltransferase; forms
                     hexamers in Bacillus subtilis; phosphoroltic 3'-5'
                     exoribonuclease; involved in maturation of tRNA precursors
                     and removes terminal nucleotides near CCA acceptor arms of
                     mature tRNAs; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HNE02703.1"
                     /translation="VGIWRGTPVLDLDYAEDSEAETDMNVVMNDAGAYIEVQGTAEGH
                     AFRAEELDAMLALARAGIAELIGHQRAALGIGR"
     gene            complement(240..1034)
                     /locus_tag="PLQ18_13440"
     CDS             complement(240..1034)
                     /locus_tag="PLQ18_13440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02704.1"
                     /translation="MHPDVILSRTGLAQTEIRDRTRQLTPRLRRLLLLVDGHTSVAGT
                     VERYGGLFGPDIGTLIDDLHSRGYLAGTAMTAAGDDAPLVPADTPLTLDAARERALEL
                     LATVVGPVGGRLGAGAARQMERLNGARDADGWRAALGELDAALPRLVGRRDLERVRTA
                     LAGLVAVQVEAASVPVTGLPAALDQVRRRTLALLAESVGPVGGRMNAAAARRIERLEA
                     VTTPEDLHAALSELESALPRLVARTELDRVRVTLGRLRRTAERAEG"
     gene            1178..>1565
                     /locus_tag="PLQ18_13445"
     CDS             1178..>1565
                     /locus_tag="PLQ18_13445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018141211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HNE02705.1"
                     /translation="MNDVPSGPRRVVLATGNAGKVRELAACLADLGCEVLPQSRFGVP
                     EAEETGLSFVENAILKARNAAAHTGLPAIADDSGIEVDALDGAPGIYSARFAGPGADD
                     AANNARLLAELAGVRRASFGPFARLWS"
BASE COUNT          219 a          563 c          571 g          212 t
ORIGIN      
        1 ggtcggcatc tggcgcggca cgccggtgct cgacctcgac tacgccgagg attccgaggc
       61 cgaaaccgac atgaacgtgg tcatgaacga tgccggcgcc tacatcgagg tgcagggcac
      121 ggccgaaggt catgcgttcc gggccgagga actcgacgcg atgctcgcgc tcgcgcgcgc
      181 cggcatcgcc gagctgatcg gccaccagcg cgccgcgctc ggcatcggcc gctgaggcct
      241 cagccttcgg cgcgctccgc ggtgcggcgc agacggccga gcgtgacgcg cacgcgatcc
      301 agttcggtac gcgcgaccag ccgcggcagc gccgattcga gttcggagag cgccgcgtgc
      361 agatcctcgg gcgtcgtgac cgcttcgagc cgctcgatcc gccgcgccgc cgcagcgttc
      421 atgcgtccgc cgaccggacc gacggattcg gcgagcagcg ccagcgtgcg ccggcgcacc
      481 tgatcgagcg cggccggcag gcccgtgacc ggcacgctgg cggcctcgac ctgcacggcc
      541 accagcccgg ccagggccgt gcgcacacgt tcgagatcgc gccgcccgac cagccggggc
      601 agcgcggcat cgagttcgcc gagggcggcc cgccagccgt cggcatcgcg ggcgccgttc
      661 agacgctcca tctgccgcgc cgcgccggcg ccgagccggc caccgaccgg cccgaccacc
      721 gtggccagca gttcgagcgc gcgctcgcgc gcggcgtcga gcgtcagcgg cgtgtccgcc
      781 ggcacgagcg gcgcgtcgtc gccggccgcg gtcatcgccg tgccggccag gtagccgcgg
      841 ctgtgcaggt catcgatcag cgtgccgatg tccgggccga acagaccgcc gtagcgctcg
      901 acggtgccgg cgacgctggt gtgcccgtcc acgagcagca gcaggcggcg caggcggggg
      961 gtgagctgac gggtgcgatc gcggatttcg gtctgtgcga gaccggtccg gctcaggatg
     1021 acatcggggt gcatggcggg cggccctcgg tctgatcggt ctgtctgaag ccgtgctgcc
     1081 cggttatttt ttccgtgtcc cgggcagccg gatcatgaac cggccgctgc aatgcaacaa
     1141 gccgagcgaa accctgattc agaacgagga aattccgatg aacgatgtac cgtccggacc
     1201 gcgccgcgtc gtgctcgcga ccggtaacgc cggcaaggtg cgcgagctgg ccgcctgcct
     1261 cgccgatctc ggctgcgaag tcctgccgca gtcgcgcttc ggtgtgcccg aagccgagga
     1321 gaccgggctg agcttcgtcg agaacgcgat cctgaaggcg cgcaacgccg ccgcacacac
     1381 cggcctgccg gcgatcgccg atgactccgg catcgaggtc gacgcgctcg acggggcgcc
     1441 gggcatctat tcggcgcgct tcgccgggcc gggcgccgat gacgccgcca acaacgcccg
     1501 gctgctggcg gagctggccg gcgtgcgccg cgcaagtttc gggccgtttg ctcgattgtg
     1561 gtcag
//
LOCUS       DAOQJH010000704         1563 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_74774_length_1563_cov_3.350796, whole genome shotgun sequence.
ACCESSION   DAOQJH010000704 DAOQJH010000000
VERSION     DAOQJH010000704.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1563
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74774_length_1563_cov_3.350796"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..379)
                     /locus_tag="PLQ18_13450"
     CDS             complement(<1..379)
                     /locus_tag="PLQ18_13450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02706.1"
                     /translation="MSASSRPPVLALAVLLLAAGSSAAAQMPLPRVDYSAVLRVSGAP
                     APVRVAHHAGVLRSEGQVGGRAVVGLLDLASGRVTLLPAGGSGPAVQLPPGTPVPGLA
                     DFDLRAFALGPARGRSQVAGHACT"
     gene            378..695
                     /locus_tag="PLQ18_13455"
     CDS             378..695
                     /locus_tag="PLQ18_13455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02707.1"
                     /translation="MGAALVDDQGTDGGRLDKAVDNRRRCMVFCGHHADANHCHEPAT
                     ALPDPLRRIGQRLFLQVTRPGNPVRIMCCNIRNTFLRVSPPFPVCHRFVPGLLRLRSG
                     RRA"
     gene            805..>1563
                     /locus_tag="PLQ18_13460"
     CDS             805..>1563
                     /locus_tag="PLQ18_13460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013812413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HNE02708.1"
                     /translation="MTPPAGKYRPFRPLTLPDRQWPSRSIERAPRWCSSDLRDGNQAL
                     IEPMDGARKQRYFEMLVRMGFKEIEVAFPSASQTDFDFVRSLIEGGHVPDDVAIQVLT
                     QAREDLIRRTFASLKGVKRAIPHFYNATSPIFRETVFGLDRAGTIALAVRGARLFAEC
                     AAEQPETEWTFEYSPETFSFTEPGFALEICEAVLEVWQPTPQRPVILNLPATVEMATP
                     NVYADQIEWFCRHVSRRDSVIISVHPHNDRGTAVA"
BASE COUNT          245 a          566 c          530 g          222 t
ORIGIN      
        1 gcgtgcaggc atggccggcg acctggctgc ggccgcgcgc cggaccgagc gcgaaggcgc
       61 gcaggtcgaa atcggcgagg ccgggcaccg gcgtgcccgg cggcagctgt acggccggac
      121 cgctgccgcc ggccggcaac agcgtcacgc gcccgctggc gagatcgagc aggccgacca
      181 cggcgcgccc gccgacctgg ccttcgctgc gcagcacgcc ggcgtgatgg gcgacgcgca
      241 ccggcgcggg cgcgccggag acgcgcagca cggcgctgta atcgacgcgc ggcagcggca
      301 tctgcgctgc ggcgctgctg ccggcggcaa gcagcaacac ggcgagcgcc agcacgggcg
      361 gacgggagct tgcggacatg ggtgcggccc tggtggacga tcaaggaacg gatggcggac
      421 ggctggacaa agccgttgac aatcgccgcc ggtgcatggt tttctgcggg catcatgcag
      481 atgcgaacca ttgccacgaa cctgcgactg ccctgcccga tcctctgagg aggatcgggc
      541 agcggctgtt cctgcaggtt acccgcccag gcaatccggt acgcatcatg tgctgcaaca
      601 tccgtaacac cttccttcgc gtttcgcccc ccttccctgt ttgccatcgg ttcgttccgg
      661 gcctgctgcg actgcgcagc ggtcgccggg cctgacggct gcccggcggc cggccgtgca
      721 cctgccgtgt cccgcatgca taccgaaccg aacacgccgc ccgagacgaa tccgatgaac
      781 cacacgaacc cgaacgtttc cgcgatgacc ccgcccgccg gcaagtaccg gccgttccgg
      841 ccgctgacgc tgcccgatcg ccagtggccg tcgcgcagca tcgagcgcgc gccgcgctgg
      901 tgctcgtccg acctgcgcga cggcaaccag gcgctgatcg agccgatgga cggcgcgcgc
      961 aagcagcgct acttcgagat gctggtgcgc atgggcttca aggagatcga ggtcgccttc
     1021 ccgtcggcct cgcagaccga tttcgacttc gtgcgcagcc tgatcgaggg cggccacgtg
     1081 cccgatgacg tcgcgatcca ggtgctgacg caggcgcgcg aggacctgat ccgccgcacc
     1141 ttcgcgagcc tgaagggcgt gaagcgtgcg atcccgcact tctacaacgc cacctcgccg
     1201 atcttccgcg agaccgtgtt cggactcgat cgcgccggca cgatcgcgct cgcggtgcgc
     1261 ggcgcgcggc tgttcgccga atgcgcggcc gagcagcccg aaaccgagtg gaccttcgag
     1321 tattccccgg aaaccttctc gttcaccgag cccggcttcg cgctcgagat ctgcgaggcg
     1381 gtgctcgaag tctggcagcc gacgccgcag cggccggtga tcctgaacct gccggcgacc
     1441 gtcgagatgg cgacgccgaa cgtctacgcc gaccagatcg aatggttctg ccggcacgtc
     1501 tcgcgccgcg acagcgtcat catcagcgtg catccgcaca acgaccgcgg caccgccgtc
     1561 gcc
//
LOCUS       DAOQJH010000705         1562 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_74845_length_1562_cov_4.740544, whole genome shotgun sequence.
ACCESSION   DAOQJH010000705 DAOQJH010000000
VERSION     DAOQJH010000705.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1562
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74845_length_1562_cov_4.740544"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1143)
                     /locus_tag="PLQ18_13465"
     CDS             complement(<1..1143)
                     /locus_tag="PLQ18_13465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNE02709.1"
                     /translation="MSFSELGLAPEIVRAVAERGYDTPTPIQRQAIPAVLSGGDLLAG
                     AQTGTGKTAGFTLPILQLLSTRPSRHPGPRAPIRALILTPTRELAAQVEDSVRAYGRH
                     LPLRSMTVFGGVGMNPQVEQLKTRIDILVATPGRLLDHVQQRTVELGHVEILVLDEAD
                     RMLDMGFIRDIRRVLALLPKQRQNLLFSATFSDDIRKLADGLLDRPAMVEVAPRNATA
                     DRVEQQVYRVERASKRHLLAHLVRSQDWHQVLVFVRTKHGANRLAEQLSKSGLPALAL
                     HGDKSQSARTRALASFKDGSLRILVATDIAARGIDISELPHVVNFDLPNVAEDYVHRI
                     GRTGRAGSSGAAVSLVSAEEQPLLAAIEKLIRRPLPIQVHPEFEADD"
BASE COUNT          278 a          483 c          539 g          262 t
ORIGIN      
        1 atcgtccgct tcaaactccg gatgaacctg gattggcagc gggcgacgga tcagcttctc
       61 gatggcggcg agcagcggct gctcctcggc gctgaccagc gacaccgcag ccccgctgct
      121 gccggcacgg ccggtgcgcc cgatgcggtg cacgtaatcc tcggccacgt tcggcaggtc
      181 gaaattgacc acgtgcggca gttcgctgat gtcgatgccg cgggccgcga tgtcggtcgc
      241 caccagaatc cgcagcgaac cgtccttgaa cgaggccagc gcccgggtgc gggccgactg
      301 gctcttgtcc ccatgcaggg ccagggccgg caggccggac tttgacaact gctcggcgag
      361 gcggttggcg ccatgcttgg tacgcacgaa caccagcacc tgatgccagt cctggctgcg
      421 gaccagatgg gcgagcagat gacgcttgct cgcccgctcg acccggtata cctgctgctc
      481 gacccgatcg gccgtggcat tgcgcggcgc gacctcgacc atggccggtc gatcgagcag
      541 accatcggcg agcttgcgga tatcgtcgga aaacgtcgct gaaaacagca ggttctgacg
      601 ctgcttcggc agcagtgcga gaacccggcg gatgtcgcgg atgaagccca tgtcgagcat
      661 gcggtcggct tcgtcgagca cgaggatctc gacatgcccg agctcgacgg tccgctgctg
      721 cacatgatcg agcagtcgcc cgggcgtggc gaccagaatg tcgatgcggg tcttcagctg
      781 ttcgacctgc ggattcatgc cgaccccgcc gaacaccgtc atcgagcgca ggggcagatg
      841 acgcccgtag gcacgcacgc tgtcctcgac ctgggcggcc agctcccggg tcggagtcag
      901 gatcagcgcg cggatcggcg cgcggggtcc gggatgacgg ctggggcggg tcgacagcag
      961 ctgcaggatc ggcagcgtga aaccggccgt cttgccggtg ccggtctggg cgccggcaag
     1021 cagatctccg ccggacagca ccgccgggat cgcctggcgc tggatcggtg tcggcgtgtc
     1081 atagccacgc tcggccacgg cacggacgat ttcaggggca agccccagct cggaaaagga
     1141 catgtgcagg catccgttgt acggcattca ggccatcagg tccgggagac gagggacagg
     1201 cggaccagac tcggtggcgg atcgcccctc cgccgggaac cgcgcagtat ccatgtatct
     1261 gcgcgattga ccaagtgttt tcatcggaat aagacctgct gaactcccgg tggaaccggg
     1321 tactccgggc agcctggttg cactttgatt gcgaatccgg gcttgacgac cggatcgcga
     1381 ttcgtagaat gcgcggctcg ctgcagtcag ccgggtcaaa ccggggcctg cagcaggcgg
     1441 tgcggattga cgaaagtttc tcttgtacat gcgtcacacg gacgcttgac gggatgaaaa
     1501 cggtcagtag aatgcgcggc ctcgctggaa ggcgggaatg attgactggc ggtaatggtt
     1561 ga
//
LOCUS       DAOQJH010000706         1556 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_75497_length_1556_cov_3.194537, whole genome shotgun sequence.
ACCESSION   DAOQJH010000706 DAOQJH010000000
VERSION     DAOQJH010000706.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1556)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75497_length_1556_cov_3.194537"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(20..1450)
                     /locus_tag="PLQ18_13470"
     CDS             complement(20..1450)
                     /locus_tag="PLQ18_13470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02710.1"
                     /translation="MPPARLLPSLSVLLLAVSGCATLPPAPGAATIPAECTTLVAALD
                     AAAPGSPSTPRAVPGWPGLAADRFLAGFDPATLPAPAIDAWRQRLAGAGREREALQRA
                     RLPDASPAPLAPVATLAARLDACRTASLAAAESPAALARLHAAVQVPSDYSTVARVLG
                     LWPLSVLPVQALGVRYRQETLARFADPPPSPATTQRFGPLPAPPQAVGRLRRDALDVP
                     VPTAAQLDALFALHAPVFEIDGRTDADLPGRPLRRADGRLDFRPEPRVYQWPSLSRLH
                     GRPVLQLNWLIWFAARPPAAGALYAGRLDGLIWRVTLDADGRPYVHDSIHACGCYHLL
                     FPRAGLRLTAAAAALAETPITPYPAPAFRPGRHLRLRIASATHYLIALDEDDGRPFVP
                     YRSVDYTALYTDTDADADAGTQTLFDPDGLIPGSERPERWLLWPMGAASAGAMRERGR
                     HAIAFVGERHFDDPGLPADWLEESPP"
BASE COUNT          226 a          532 c          638 g          160 t
ORIGIN      
        1 agcaggggca ggcggggatt catggcgggc tttcctccag ccagtcggcc ggcaggccgg
       61 gatcgtcgaa gtggcgttcg ccgacgaagg cgatcgcatg ccggccgcgt tcgcgcatcg
      121 cgccggccga ggccgcgccc atcggccaca gcagccagcg ctccgggcgc tcgctgccgg
      181 gaatgagacc gtcgggatcg aacaaggtct gcgtgccggc atcggcatcg gcatcggtat
      241 cggtatacag ggcggtgtaa tcgacgctgc gatacggcac gaacgggcgg ccgtcgtcct
      301 cgtcgagcgc gatcaggtaa tgcgtggcgc tggcgatgcg caggcgcaga tgacgaccgg
      361 ggcgaaacgc cggcgccgga tacggggtga tcggcgtttc cgccagcgcc gcggcggcgg
      421 ccgtcaggcg caggccggcg cgcgggaaca gcaggtggta gcagccgcag gcgtggatcg
      481 agtcatggac atacgggcgg ccgtcggcgt cgagcgtcac gcgccagatc aggccgtcga
      541 ggcggccggc gtacagcgca cctgcggctg gcgggcgcgc ggcgaaccag atcagccagt
      601 tcagctgcag caccgggcgg ccgtgcaggc ggctcagcga cggccactga tagacgcgcg
      661 gctcgggccg gaagtcgagg cggccgtcgg cgcggcgcag cggccggccg gggagatcgg
      721 catccgtgcg gccgtcgatc tcgaacaccg gcgcgtgcag cgcgaacagc gcatcgagct
      781 gggcggcggt cggcaccggc acatcgagcg catcgcggcg cagccggccg accgcctgcg
      841 gcggtgccgg cagcggaccg aagcgctgcg tcgtggcggg cgatggcggc ggatcggcga
      901 agcgcgccag cgtttcctgc cggtagcgca ccccgagcgc ctgcaccggc agcacgctca
      961 gcggccacag cccgagcacg cgcgccaccg tggaatagtc ggaaggcacc tgcacggcgg
     1021 cgtgcaggcg cgccagcgcc gccggtgact ccgcggccgc gaggctggcg gtgcggcagg
     1081 cgtcgagccg tgccgccagc gtcgcgacgg gcgcgagcgg ggccggggac gcatccggca
     1141 ggcgcgcacg ctgcagcgct tcgcgctcgc gcccggcccc ggccaggcgc tggcgccagg
     1201 catcgatggc cggggccggc agcgttgccg gatcgaaacc cgccagaaag cggtcggccg
     1261 ccagccccgg ccagccgggc acggcacgcg gggtcgatgg cgaaccgggc gcggcggcat
     1321 cgagcgccgc gaccagggtc gtgcattcgg ccgggatcgt ggccgcgccg ggcgcgggcg
     1381 gcagcgtcgc gcagccgctg acggcgagca gcagcacgga cagcgagggc agcaggcggg
     1441 cggggggcat gcaggaatct ccggggaggc cggtggcagg tacgcagccg gcgggtcgca
     1501 gcgatacagt gcccggcctt tgcggcctgc ggagtacacc ggcgtgcagc cgatcc
//
LOCUS       DAOQJH010000707         1535 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_77536_length_1535_cov_4.122973, whole genome shotgun sequence.
ACCESSION   DAOQJH010000707 DAOQJH010000000
VERSION     DAOQJH010000707.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1535
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77536_length_1535_cov_4.122973"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            7..822
                     /gene="mutM"
                     /locus_tag="PLQ18_13475"
     CDS             7..822
                     /gene="mutM"
                     /locus_tag="PLQ18_13475"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020485442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HNE02711.1"
                     /translation="MPELPEVETTRRGIAPHLLGRTIARVIVREPRLRWPVPPEFSAN
                     LEAQAIERVERRGKYLLLGNALGFALLHLGMSGSLRILPASTPPGRHEHLDLLLDDGL
                     CLRLTDPRRFGAALWAGPEPHAHPLLAHLGPEPLGPGFTAGGLAARAAGRSQAVKSFI
                     MDSHVVVGVGNIYASEALFLAGIDPRRPAGSVTPPAWLRLVAAIRQVLEAAIAEGGTT
                     LRDFVGVEGKPGYFRQQLRVYDRAGQPCRSCGAPIVQIRQTGRSTYFCACCQS"
     gene            961..>1535
                     /locus_tag="PLQ18_13480"
     CDS             961..>1535
                     /locus_tag="PLQ18_13480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005367804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNE02712.1"
                     /translation="MSEHVIELRSATKAYGKQTVLHGVDLKVRRGEFLTLLGPSGCGK
                     TTILRLLAGFESPTTGTVLLDGQDVTALPPEDRGVNTVFQSYALFPHMTVYDNVAFGP
                     RIKGVGGSELETRVMEALKTVKLDGFAQRKPAQLSGGQQQRVAIARALVNRPLVLLLD
                     ESLSALDYKLRKAMQIELKQLQRQLGITFVFV"
BASE COUNT          217 a          598 c          491 g          229 t
ORIGIN      
        1 gccccgatgc ccgaactgcc cgaagtcgaa accacgcgcc gcggcatcgc gccgcacctg
       61 ctcgggcgca cgatcgcgcg cgtgatcgtg cgcgagccgc gcctgcgctg gccggtgccg
      121 ccggagttca gcgccaacct cgaagcacag gcgatcgagc gcgtcgagcg ccgcggcaag
      181 tacctgctgc tcggcaacgc gctcggcttc gcgctgctgc acctcggcat gtccggcagc
      241 ctgcgcatcc tgcccgcgag cacgccgccc ggccgccacg agcatctcga cctgctgctc
      301 gacgacggcc tctgcctgcg cctcaccgac ccgcgccgct tcggcgccgc gctgtgggcc
      361 ggccccgagc cgcacgcgca tccgctgctc gcgcatctcg gccccgagcc gctaggcccc
      421 ggcttcacgg ccgggggcct ggctgcgcgc gccgccggcc gcagccaggc ggtcaagagc
      481 ttcatcatgg acagccacgt cgtcgtcggc gtcggcaaca tctacgcgtc cgaggcgctg
      541 tttctcgccg gcatcgatcc gcgccggccg gccggcagcg tcacgccgcc ggcctggctg
      601 cggctggtgg cggcgatccg tcaggtgctc gaagccgcga tcgccgaggg cggcacgacg
      661 ctgcgcgact tcgtcggcgt cgagggcaaa cccggctact tccgccagca gctgcgcgtc
      721 tacgaccgcg ccggccagcc ctgccgcagc tgcggcgcac cgatcgtgca gatccgccag
      781 accggccgat cgacctactt ctgcgcgtgc tgccagtcct gaagcccccc gccgggctgc
      841 gctgcacctg caaaaaacgc cgtcggcgga ttcgggaatt cgtgtatgtt tagccgcgct
      901 tccggtcgcc ctgcccggct gtttcacccc tgattccctc ccgccttcgc cgtccggacc
      961 atgagcgaac acgtcatcga attgcgcagt gccaccaagg cttacgggaa gcagacggtc
     1021 ctgcacggcg tcgacctgaa ggtccgccgc ggcgagttcc tgaccctgct cggcccttcc
     1081 ggttgcggca agaccaccat cctgcgcctg ctcgccggct tcgaatcccc gaccaccggc
     1141 accgtgctgc tcgacggcca ggacgtgacc gcgctgccgc ccgaggatcg cggcgtcaac
     1201 acggtgttcc agagctacgc gctgttcccg cacatgacgg tgtacgacaa cgtcgccttc
     1261 ggtccgcgca tcaagggcgt cggcggcagc gaactcgaaa cccgcgtcat ggaggcgctg
     1321 aagaccgtca agctcgacgg cttcgcgcag cgcaagcccg cgcagctctc cggcggccag
     1381 cagcagcgcg tcgccatcgc ccgcgcgctg gtcaaccggc cgctggtgct gctgctcgat
     1441 gaatcgctgt cggcgctcga ctacaagctg cgcaaggcca tgcagatcga gctgaagcag
     1501 ctgcagcgcc agctcggcat caccttcgtg ttcgt
//
LOCUS       DAOQJH010000708         1530 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_78025_length_1530_cov_4.081356, whole genome shotgun sequence.
ACCESSION   DAOQJH010000708 DAOQJH010000000
VERSION     DAOQJH010000708.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1530)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1530)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1530
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78025_length_1530_cov_4.081356"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(149..595)
                     /locus_tag="PLQ18_13485"
     CDS             complement(149..595)
                     /locus_tag="PLQ18_13485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNE02713.1"
                     /translation="MSEQTVRLHRVLRAPPGRVYRAFLDADALCRWLPPYGFLCRVHE
                     LDAQVGGHFRMSFRNFATGREHFFGGEYLELQPEQRIRYVDRFDDPNLPGLMNVTIRL
                     RAVLCGTDLQIEQGGLPEAIPVEMCYLGWQESLVQLASLVEPEIAD"
     gene            complement(656..>1530)
                     /locus_tag="PLQ18_13490"
     CDS             complement(656..>1530)
                     /locus_tag="PLQ18_13490"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNE02714.1"
                     /translation="GVSSTVLLTGESGTGKELLARGIHAASARAAGPWIALNCATLTE
                     SLQIAELFGHAKGAYTGAAGERAGLFEAASGGTLFLDEVGELSPSAQAALLRVLQEGV
                     IVRVGEHRERPVDVRIVAATHRDLEARVGAGQFRADLFYRLNVVSIRMPALRERDNDV
                     LLLAQSLLERYGARFGRNVQALSPEVLRLFLAHPWPGNVRELANVLERAVLLARGTRI
                     ELEDLPDGFAAAVHGDGAAAALPGAPEVIGQQERERIRAALERSHGRRDVAATLLGIS
                     RATLWRRMKRLGLA"
BASE COUNT          229 a          534 c          564 g          203 t
ORIGIN      
        1 gcggacaaac ccttcgtgac ctgggacggg gacaaaaagg tcgtctgaag cgccgctgcg
       61 gaaagccctg ccagatcagt ctggtccggt ccctctccca cgagggccgg ggggctgaaa
      121 aaaggcgagc gttccggcgt gggcggtatc agtccgcaat ctccggctcg accaggctgg
      181 cgagctgcac gagcgattcc tgccagccga ggtagcacat ctccaccggg atggcttcgg
      241 gcaggccgcc ctgctcgatc tgcagatcgg tgccgcagag caccgcgcgc agccggatcg
      301 tcacgttcat caggccgggc aggttcgggt catcgaagcg atcgacgtag cggatccgct
      361 gctccggctg cagttcgagg tattcgccgc cgaagaagtg ttcgcgtccg gtcgcgaagt
      421 tgcggaacga catgcggaag tgcccgccga cctgcgcgtc gagctcgtgc acgcggcaca
      481 ggaagccgta gggcggcagc cagcggcaca gcgcatcggc atcgaggaag gcgcggtaga
      541 cccgcccggg cggggcgcgc aacacgcggt gcaggcggac ggtttgctcg gacatgacgg
      601 ctgcgacctc ggcgggagcg gcggaaacct gcgtcagcgt agcggctgac ggccctcagg
      661 ccagtccgag gcgcttcatg cgccgccaca gcgtcgcgcg gctgatgccg agcagcgtgg
      721 ccgcgacatc gcgccggccg tggctgcgct cgagcgcggc gcggatacgc tcgcgttcct
      781 gctgcccgat gacttcgggc gcgcccggca gcgccgcggc agcgccgtcg ccgtgcacgg
      841 cagcggcgaa gccatcgggc aggtcctcca gctcgatgcg cgtgccgcgt gcgagcagca
      901 cggcgcgctc cagcacgttg gcgagttcgc ggacgttgcc gggccacgga tgcgcgagga
      961 acagccgcag cacctccggc gacagcgcct gcacgttgcg cccgaagcgc gcgccgtagc
     1021 gttcgagcag cgactgcgcg agcagcagca cgtcgttgtc gcgctcgcgc agcgccggca
     1081 tgcggatgct gacgacgttg agccggtaga agagatcggc gcggaactgc ccggccccga
     1141 cccgcgcttc gaggtcgcgg tgcgtggcgg cgacgatgcg cacgtcgacc ggccgctcgc
     1201 ggtgctcgcc gacgcgcacg atcacgcctt cctgcagcac gcgcagcagc gcggcctgcg
     1261 ccgaggggct cagctcgccg acctcgtcga ggaacagcgt gccgccgctc gcggcctcga
     1321 acaggccggc gcgctcgccg gcggcgccgg tataggcgcc cttcgcgtgg ccgaacagct
     1381 cggcgatctg caggctttcg gtcagcgtcg cgcagttgag cgcgatccac ggtccggcgg
     1441 cgcgcgccga ggcggcgtgg atgccgcgcg ccagcagctc cttgccggtg ccgctctcgc
     1501 cggtcagcag caccgtcgaa ctgacgccgg
//
LOCUS       DAOQJH010000709         1527 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_78361_length_1527_cov_3.339674, whole genome shotgun sequence.
ACCESSION   DAOQJH010000709 DAOQJH010000000
VERSION     DAOQJH010000709.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1527
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78361_length_1527_cov_3.339674"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1527)
                     /locus_tag="PLQ18_13495"
     CDS             complement(<1..>1527)
                     /locus_tag="PLQ18_13495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012631669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNE02715.1"
                     /translation="FAQLVVARRGERVIRLGQLAHVGDGAEEARSLAFYDGRPAIGIG
                     VLKSRGASTTAVSAALRAQVAQVQATLPAGVELRIVQDSGERVGHSVANVGEHLVLGA
                     LLTVLIVFLFLNSWRSTVITGLALPVSVLAAFVAVWACGFTLNQMSLLGLSLAIGILI
                     DDAIVVRENIVRHVHLGRSHLDAARAGTAEIGLAVTATTFSIVAVFVPVAFMSGVAGQ
                     WFKPFALTIACAVLVSLFVSFSLDPMLSAYWPDPQPEAQRRGWLTRALDRFNAGFDRL
                     TGRYVRVIGWALDHRLVMTALALGSFFGALWLQAQFGGAAFVPPSDRSELRIVIDTPP
                     GSSLEHTRVLAERVAGLARALPEVRYTYTRAGSAGDFTVGGVDTATVYVRLKPKSGRS
                     RTQEQIGNALRAQAQQLAGAEVAVFSGGFGGAYKPIQLRLIGPDARELAETAQRLLAA
                     AREVPGIVDANLSTRGRKPEYELTVDRGLAGALGITVADIGQGLRVAYAGLEAGDWID
                     "
BASE COUNT          250 a          547 c          552 g          178 t
ORIGIN      
        1 ggtcgatcca gtcgccggct tcgagtccgg cataggcgac gcgcagcccc tggccgatgt
       61 cggcgaccgt gatgccgagc gcaccggcga gcccgcgatc gaccgtcagc tcgtactcgg
      121 gcttgcgccc gcgcgtcgag aggttcgcat cgacgatgcc cggcacctcg cgcgccgccg
      181 cgagcaggcg ctgggcggtc tcggcgagtt cgcgcgcatc cgggccgatc agtctgagct
      241 ggatcggctt gtaggcgccg ccgaagccgc ccgagaacac cgcgacctcg gcgccggcga
      301 gctgctgcgc ctgcgcgcgc agtgcgttgc cgatctgctc ctgcgtgcgc gagcgtccgg
      361 acttgggttt caggcgcacg tagacggtcg cggtatcgac gccgccgacc gtgaaatcgc
      421 cggcgctgcc ggcgcgcgta taggtgtagc gaacctcggg cagcgcgcgg gcaagcccgg
      481 cgacgcgctc ggccagcacg cgcgtgtgtt cgaggctgga gccgggtggc gtgtcgatga
      541 cgatgcgcag ctcgctgcgg tcgctcggcg gcacgaaggc cgcaccgccg aactgcgcct
      601 gcagccacag cgcgccgaag aagctgccga gcgcgagcgc cgtcatcacc agccggtgat
      661 cgagcgccca gccgatcacg cgcacgtagc gtccggtcag ccggtcgaag ccggcgttga
      721 agcgatcgag cgcgcgcgtc agccagccgc gccgctgcgc ctcgggctgc ggatcgggcc
      781 agtaggccga cagcatcgga tcgagcgaga acgacacgaa cagcgacacc agcaccgcgc
      841 aggcgatggt cagcgcgaag ggcttgaacc actggccggc gacgccggac atgaacgcca
      901 ccggcacgaa caccgcgacg atggaaaacg tggtcgcggt caccgccagc ccgatctcgg
      961 cggtgccggc gcgcgcggca tcgaggtgcg aacggcccag gtgcacatgg cgcacgatgt
     1021 tctcgcgcac gacgatggcg tcatcgatca ggatgccgat cgcaagcgac agcccgagca
     1081 gcgacatctg gttcagcgtg aagccgcagg cccagaccgc gacgaaggcc gcgagcaccg
     1141 acaccggcag cgccagaccg gtgatgacgg tcgagcgcca cgagttcagg aacaggaaca
     1201 cgatcagcac cgtcagcagc gcgccgagca cgaggtgctc gccgacgttg gcgaccgaat
     1261 ggccgacgcg ctcgccggag tcctgcacga tgcgcagctc cacgccggcc ggcagcgtcg
     1321 cctgcacctg cgcgacctgc gcgcgcagcg cggcgctgac cgccgtcgtg ctggcgccgc
     1381 gcgacttcag cacgccgatg ccgatcgcgg ggcggccgtc gtagaaggcg agcgaacgcg
     1441 cctcctcggc gccgtcgccg acgtgcgcga gctgcccgag ccggatcacg cgctcgccgc
     1501 ggcgcgcaac cacgagctgc gcgaagg
//
LOCUS       DAOQJH010000710         1505 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_80678_length_1505_cov_4.573103, whole genome shotgun sequence.
ACCESSION   DAOQJH010000710 DAOQJH010000000
VERSION     DAOQJH010000710.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1505
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80678_length_1505_cov_4.573103"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..752)
                     /locus_tag="PLQ18_13500"
     CDS             complement(<1..752)
                     /locus_tag="PLQ18_13500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007351738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HNE02716.1"
                     /translation="MQTTSSIQVIDRLARLLDAIAANNNPVSLKVLSAETGLHPSTAF
                     RILASLAEHGFVERSSSGHYRLGVKLLQLGSRVQGRLDIRREARPIMEWLRNETGETV
                     NLIVREGDEVVYVERVVPNRMMRVEQMIGGRAPLHVTAVGKLFLAEGGAEACLEYAAR
                     TGLPSCTPHSITDPTALWRSVKNALQQGYALDDQEAELGVGCIGVPIRDSTNHIVAGI
                     SVSAPIERRREVWVTLIRQAGEKLSTRLGFHP"
     gene            1063..>1505
                     /locus_tag="PLQ18_13505"
     CDS             1063..>1505
                     /locus_tag="PLQ18_13505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014590532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HNE02717.1"
                     /translation="MTDDATRALRDAIARGVPERLILADIEDLRPYECDGLTAYRTVP
                     RLVVLPETAEQAAHVLATCHERGVPVVARGAGTGLSGGAMPHADGVVLSLAKFNRILG
                     IDPQNRTARVQPGVRNIAISQAAAPLGLYYAPDPSSQIACTIGGNV"
BASE COUNT          258 a          497 c          502 g          248 t
ORIGIN      
        1 tccggatgga atccgagccg cgtcgacagc ttctcgccgg cctgacggat cagcgtcacc
       61 cagacctcgc ggcggcgttc gatcggggcc gagaccgaga tgccggcgac gatgtggttc
      121 gtcgagtcgc ggatcggcac gccgatgcag ccgacgccga gttcggcttc ctggtcatcg
      181 agcgcgtagc cctgctgcag cgcgttcttg accgagcgcc acagcgccgt cgggtcggtg
      241 atcgagtgcg gcgtgcacga gggcaggccg gtgcgcgcgg cgtattcgag acaggcttcg
      301 gcgccgccct cggcgaggaa cagcttgccg accgcggtga cgtgcagcgg cgcccggccg
      361 ccgatcatct gctcgacgcg catcatgcgg ttcggcacca cgcgctcgac gtacacgacc
      421 tcgtcgccct cgcgcacgat caggttgacc gtctcgccgg tctcgttgcg cagccattcc
      481 atgatcgggc gcgcctcgcg gcggatgtcg agccggccct gcacgcgcga gccgagctgc
      541 agcagcttca cgcccagacg gtagtggccc gaggaactac gttcgacgaa cccgtgctcg
      601 gccagcgagg cgaggatgcg gaaggctgtc gatggatgca ggccggtctc ggccgacaac
      661 accttgaggc tgaccggatt gttgttggcg gcgattgcat ccagcagccg ggcgagccga
      721 tcaatgacct gaatggacga ggtcgtctgc atgaccattc cgaaactcca cgcgcattgc
      781 tgatcgagta aagacgtttt gcatcacgaa aaatagtcta ttgcactgca caaaaaattg
      841 ctactcctga tgcggggcat gaagatgttt tcacgatgcg aatgcggaca ggccaatttc
      901 tgctttggtc tggccattga tccagatggc tggcgttttt tcccggactg cggtaaacag
      961 tcgggcgcag aatgcacata aactccgcag ggaatgcagg gttgaagcca caccaggagc
     1021 ccgcggccgg cgcatccggt cgggagatga gaggtaagac caatgactga cgacgcgacc
     1081 cgcgcattgc gcgacgcgat agcccgcggc gtgcccgaac ggctgatcct ggccgatatc
     1141 gaggacctgc gcccctacga gtgcgatggt ctgaccgcct accgcaccgt gccgcgcctc
     1201 gtggtgctgc cggagaccgc cgaacaggcc gcgcacgtgc tcgcgacctg ccacgagcgc
     1261 ggcgtgccgg tggtcgcgcg cggggccggc accgggcttt cgggcggcgc gatgccgcat
     1321 gccgacggcg tcgtgctgtc gctcgccaag ttcaaccgca tcctcggcat cgacccgcag
     1381 aaccgtaccg cgcgcgtgca gccgggcgtg cgcaacatcg cgatttcgca ggccgccgcg
     1441 ccgctcggcc tgtactacgc gccggacccg tcctcgcaga tcgcctgcac gatcggcggc
     1501 aacgt
//
LOCUS       DAOQJH010000711         1500 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_81201_length_1500_cov_4.250519, whole genome shotgun sequence.
ACCESSION   DAOQJH010000711 DAOQJH010000000
VERSION     DAOQJH010000711.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1500)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1500)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1500
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81201_length_1500_cov_4.250519"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..397)
                     /locus_tag="PLQ18_13510"
     CDS             complement(<1..397)
                     /locus_tag="PLQ18_13510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006748563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HNE02718.1"
                     /translation="MSRPLPARDEVWSAEDYLGWPSDTPRCELIDGRVYAMSPAPTLD
                     HQRITGALFAALRSTLAERRGRGSGGAGCGCEVLMAPVDVRLDAHTVVQPDVLVVCDP
                     AKLANGRHVDGAPDLVVEVLSPSNALKDRR"
     gene            complement(477..>1500)
                     /locus_tag="PLQ18_13515"
     CDS             complement(477..>1500)
                     /locus_tag="PLQ18_13515"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNE02719.1"
                     /translation="TRFLFVSIALFYFSGRPGEQPFWYPVWAVWLHVGSYWLLNAVNM
                     WHAWLGRYGESRIRLAMVLDVASISLFLVNDPYSVPPSLLAYLMVILGNGMRYGLRMY
                     GESLAWSFGCALLALVVRYQDSLTDLRAGLIFLPLFSAIIVMYAYFLLLRLEQARVQL
                     ERVSRYDGLTGLLNRRGLAESVELLQRLMDRDTDRITVVFADMDRFKSVNDTLGHTAG
                     DAVLQEIGRILREGVRRSDLIARYGGDEFVFVLPHTGREQALQVTGRVERRIHELSRR
                     TGIDFGITFGMGETGAGHRADVLELIDAVDREMYEAKRARRQQRETTLRADAAPAWDT
                     PAAGGG"
BASE COUNT          256 a          510 c          532 g          202 t
ORIGIN      
        1 gccgccggtc cttcagcgcg ttcgacggcg acagcacctc gacgaccaga tcgggtgcgc
       61 catcgacatg gcggccattg gcgagcttcg ccgggtcgca gacgaccagc acgtcgggct
      121 gcacgacggt gtgggcgtcg agacgcacgt ccacgggggc catcaacact tcgcagccac
      181 agccggcacc gccggaacca cgtccgcggc gctcggcgag tgtcgatcgc agcgcggcaa
      241 acagggcacc ggtgatgcgc tgatgatcca gcgtcggtgc cggcgacatc gcatacacgc
      301 gcccgtcgat cagctcgcag cgcggcgtgt cgctcggcca gccgagatag tcctcggcgc
      361 tccagacttc gtcacgggcg ggcagcgggc gggacatggt cgggtacacc tgcggagtcg
      421 gccgaagcgg gatggcgcga tgctaggccg ctgccgcgca gccggccagt ggcgcctcag
      481 ccgccgcccg cggcgggagt gtcccaggcc ggagccgcat cggcccgcag cgtggtctcg
      541 cgctgctggc ggcgtgcgcg cttggcctcg tacatctcgc gatcgaccgc gtcgatcagt
      601 tcgagcacat cggcgcgatg cccggcaccg gtctcgccca tgccgaaggt gatgccgaag
      661 tcgatgccgg tgcgccggga aagctcgtgg atgcgccgct cgacccggcc ggtgacctgc
      721 agcgcctgtt cgcggccggt gtgcggcagc acgaacacga actcgtcgcc gccgtagcgg
      781 gcgatcaggt cggagcggcg cacgccctcg cgcaggatgc ggccgatctc ctgcagcacc
      841 gcgtcgccgg cggtgtggcc gagcgtgtcg ttgaccgact tgaagcggtc catgtcggcg
      901 aacacgacgg tgatgcggtc ggtgtcgcga tccatcagcc gctgcagcag ctcgaccgat
      961 tcggcgaggc cgcgacggtt cagcaggccg gtcaggccgt cgtagcggct gacgcgttcg
     1021 agctgcacgc gcgcctgttc gaggcgcagc agcaggaagt acgcgtacat gacgatgatc
     1081 gcgctgaaca gcggcaggaa gatcagcccg gcgcgcaggt cggtcagcga gtcctgatag
     1141 cgcacgacga gcgcgagcag cgcgcagccg aagctccagg ccagcgattc gccgtacatg
     1201 cgcagcccgt aacgcatgcc gttgccgagg atgaccatca gataggcgag cagggacggc
     1261 ggcacgctgt acggatcgtt gaccaggaac agcgagatgc tggccacgtc gagcaccatc
     1321 gccagccgga tgcgcgattc gccatagcgc ccgagccagg cgtgccacat gttcacggcg
     1381 ttcagcagcc agtagctgcc gacgtgcagc cacacggccc agaccggata ccagaacggc
     1441 tgctcgcccg gccggccgct gaagtagaac agcgcgatgc tcacgaacag aaagcgcgtc
//
LOCUS       DAOQJH010000712         1496 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_81644_length_1496_cov_5.459403, whole genome shotgun sequence.
ACCESSION   DAOQJH010000712 DAOQJH010000000
VERSION     DAOQJH010000712.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1496)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1496)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1496
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81644_length_1496_cov_5.459403"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(17..910)
                     /locus_tag="PLQ18_13520"
     CDS             complement(17..910)
                     /locus_tag="PLQ18_13520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNE02720.1"
                     /translation="MKRLLIVGCGDVAWRALPRLRNRYRVFSLLRDPLQAPRWRGAGA
                     TPIIADLDDPASLHRIRGLADDVLHLAPPPDRSDRDARTQALLAALERGKSLPQRLVY
                     VSTSGVYGDCGGALVDETRPSNPLSARGARRVDAERQLRAFGRRTGAIVSILRAPGIY
                     AADRLPLERLKKGLPALCDEDDVFTNHIHADDLAAACAAALDHGRAGRSYNVSDDSEL
                     RMGEYFDHVADAFGLPRPPRISRAEAERVMSPVQLSFMRESRRLVNRRLKEELGLRLA
                     YPSVDDGIRAALNETRNPSCS"
     gene            964..>1496
                     /locus_tag="PLQ18_13525"
     CDS             964..>1496
                     /locus_tag="PLQ18_13525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HNE02721.1"
                     /translation="MSFTVTIQPSGHTLSTEAGESILEAALRQGLTLPYGCRDGACGA
                     CRGKVLSGEVDHGKAQDHALSQADREQGLALFCCASPRSDLVIESREVRKAGDIPVKT
                     LPVRVQSLERAAPDVMIVKLKLPASERLQFLAGQYVDILLKDGRRRSFSLANAPHDDA
                     LLELHIRHVPGGLFTEQV"
BASE COUNT          254 a          483 c          523 g          236 t
ORIGIN      
        1 atgtgcaggg ccttgatcac gagcatgacg gattccttgt ttcgttgagg gcggcacgga
       61 tgccgtcgtc gaccgagggg taggcgaggc gaagccccag ttcttccttg aggcgccgat
      121 tgacgagccg gcgcgattct cgcatgaagg aaagctgcac gggcgacatc acgcgttcgg
      181 cctcggcccg cgaaatcctt ggcgggcggg gcaggccgaa ggcgtcggcg acgtggtcga
      241 agtactcgcc catgcgcagt tccgaatcgt cgctgacatt gtagctgcgg ccggcgcggc
      301 catgatccag tgcggcggca caggccgcgg cgagatcgtc ggcgtggatg tggttcgtga
      361 acacgtcgtc ctcgtcacac agggcgggca gccccttctt caggcgctcg agcggcaggc
      421 ggtcggcggc atagatgccc ggcgcacgca ggatggaaac gatggcgccg gtacggcgcc
      481 cgaaggcccg caactggcgc tcggcatcga cgcggcgggc gccgcgggcg ctgagcggat
      541 tcgacgggcg tgtttcgtcg accagggcgc cgccacagtc gccgtaaacc ccgctggtgc
      601 taacgtagac gaggcgctgt ggtagacttt ttcctcgctc gagtgccgcc agaagcgcct
      661 gcgtgcgggc atcccggtca ctccggtcgg gtggcggcgc aaggtgcagg acgtcgtcgg
      721 cgaggccacg gatccgatgc aggctggccg ggtcatcgag gtcggcaatg atcggcgtcg
      781 caccggcgcc acgccagcgt ggcgcctgca gcggatcacg caacagggag aatacgcggt
      841 agcggtttcg cagccggggc agggcccgcc aggcaacgtc gccacaaccg acgatcagaa
      901 gtcttttcac cggcgaattg tagcaatccg gcgccgcgtg caggcaagca acgaagggga
      961 atcatgagct tcaccgtcac catccagcca tcgggccaca cgctgagcac cgaggccgga
     1021 gaatccattc tcgaagcggc cctgcgccag ggcctgacgc tgccttacgg ttgccgcgat
     1081 ggcgcctgcg gcgcctgtcg cggcaaggtc ctgagcggcg aggtcgatca cggcaaggcg
     1141 caggaccatg ccctgtcgca agccgaccgt gagcagggac tggcgctgtt ctgctgcgcc
     1201 tcgccacgct ccgacctggt catcgaatcg cgggaagtgc gcaaggccgg tgacattccg
     1261 gtcaagacgc tgccggtgcg ggtgcagtcg ctcgaacgtg ccgctcccga cgtgatgatc
     1321 gtcaagctga agttgcccgc tagcgaacgc ctgcagtttc tcgccggaca gtacgtcgac
     1381 atcctgctca aggacggccg ccggcgcagc ttttcgcttg ccaacgcgcc gcacgacgac
     1441 gcgctgctcg aactgcacat ccgccacgtt cccggcggcc tgttcacgga acaggt
//
LOCUS       DAOQJH010000713         1494 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_81907_length_1494_cov_2.803336, whole genome shotgun sequence.
ACCESSION   DAOQJH010000713 DAOQJH010000000
VERSION     DAOQJH010000713.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1494)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1494)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1494
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81907_length_1494_cov_2.803336"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..566
                     /gene="coaE"
                     /locus_tag="PLQ18_13530"
     CDS             <1..566
                     /gene="coaE"
                     /locus_tag="PLQ18_13530"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HNE02722.1"
                     /translation="AAAAFTRLGVPVIDTDLLAREVVARGSDGLAEIVTAFGPGLLTA
                     DGALDRAAMRRLVFADAAARQRLEAITHPRIRALAAERVAALAGSGAPYCVLVVPLLL
                     ESGAYGTLCSRVLVIDVPPETQLERVLARDGSDESMAHAILAAQMPRAERLAAADDVL
                     VNTGTLEQLNEAVTALDRRYQALHAGA"
     gene            662..1429
                     /gene="zapD"
                     /locus_tag="PLQ18_13535"
     CDS             662..1429
                     /gene="zapD"
                     /locus_tag="PLQ18_13535"
                     /inference="COORDINATES: protein motif:HMM:NF003656.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HNE02723.1"
                     /translation="MDRCVWYEQPLNERMRVLLRLEFLYERVLHAAAGWSVWEARAAL
                     ESLIELLGLLGRAEVRAELLKEADRLCLQLDRQRETAADPAAVDASIRDLAISADAAR
                     RLDALQLDDIRRNEFLNALRQRASIPGGACSFDLPALHHWLNRDPEIRADQLDGWLTP
                     LLPGRDAAAAVLRTIRAAAEADPQVAQSGFYQQGLDPALPAQLVRVGVTVESGVFPEI
                     SGGRHRFTIRFLCQPNPVRRPFQTDEDTGFLLACCVV"
BASE COUNT          200 a          566 c          508 g          220 t
ORIGIN      
        1 ccgcagccgc cgcgttcacg cggctcggcg tgccggtcat cgataccgat ctgctcgcgc
       61 gcgaggtcgt cgccagaggc agcgacggac tcgccgaaat cgtcaccgcc ttcggccccg
      121 ggctgctgac ggccgacggt gcgctcgatc gtgccgcgat gcgccggctg gtgttcgccg
      181 atgcggccgc ccgccagcgg ctggaagcga tcacgcatcc gcgcatccgc gccctggccg
      241 ccgaacgcgt cgcagcgctg gccggcagcg gtgcgcccta ctgcgtgctg gtcgtgccgc
      301 tgctgctcga aagcggcgcc tacggcacgc tgtgctcgcg cgtgctggtc atcgacgtgc
      361 cgcccgaaac ccagctcgaa cgtgtgcttg cgcgcgatgg cagcgatgaa tccatggcgc
      421 atgcgatcct cgccgcgcag atgccgcgcg ccgagcgcct ggccgccgcc gacgacgtgc
      481 tggtcaacac cggcacgctc gaacagctga acgaggcggt cacggccctt gaccggcgtt
      541 accaggccct gcacgccggc gcttgacacc ccgccccggc gcgcaccggt gacatccggc
      601 gtttgccggc cccgcccggc ttcgcgacaa tacggccccc ttccggagcc gaggtcaccc
      661 cgtggaccgc tgcgtctggt acgaacagcc gctgaacgag cgcatgcgcg tgctgctgcg
      721 gctggaattc ctttacgagc gcgtgctgca cgccgccgcc ggctggtcgg tctgggaggc
      781 gcgggccgcg ctcgaaagcc tgatcgaact gctcggcctg ctcgggcgcg ccgaggtgcg
      841 cgccgaactg ctgaaggaag ccgaccggct gtgcctgcag ctcgatcgcc agcgcgagac
      901 ggccgccgat ccggccgccg tcgatgcaag catccgcgac cttgccatca gcgccgatgc
      961 cgcgcgccgg ctcgacgcgc tgcagctcga tgacatccgc cgcaacgaat tcctgaacgc
     1021 gctgcgccag cgcgccagca tcccgggcgg tgcgtgttcg ttcgacctgc cggcgctgca
     1081 ccactggctg aaccgtgatc cggaaatccg tgccgatcag ctcgacggct ggctgacccc
     1141 gctgctgccg gggcgtgacg ccgcggcagc cgtgctgcgc acgatccgcg ccgccgccga
     1201 ggccgatccg caggtcgcgc agtccggctt ctaccagcag ggcctcgatc cggcgctgcc
     1261 ggcgcagctg gtgcgcgtcg gcgtcaccgt cgaatccggg gtgttcccgg agatcagcgg
     1321 cggccggcac cgcttcacga tccgcttcct gtgccagccg aatccggtgc gccggccgtt
     1381 ccagaccgac gaggacaccg gcttcctgct cgcctgctgc gtcgtctgaa atttaccgtt
     1441 cgcagcaggc gaggcgccgg atcagagcag cacgcgctcg atgccgccgc tgcg
//
LOCUS       DAOQJH010000714         1493 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_82004_length_1493_cov_3.051460, whole genome shotgun sequence.
ACCESSION   DAOQJH010000714 DAOQJH010000000
VERSION     DAOQJH010000714.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1493
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82004_length_1493_cov_3.051460"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="PLQ18_13540"
     CDS             complement(<1..192)
                     /locus_tag="PLQ18_13540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02724.1"
                     /translation="MNKVLMTIPMVIVLAGAGLQDAQAAAYSTQDLAESFCLSTNNDL
                     FHVPSRDLGKTCSCVGVVAN"
     gene            complement(641..>1493)
                     /locus_tag="PLQ18_13545"
     CDS             complement(641..>1493)
                     /locus_tag="PLQ18_13545"
                     /inference="COORDINATES: protein motif:HMM:NF013109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HNE02725.1"
                     /translation="AQTFAAPRRERIDPAQELRGLGLANLAAAFSGAYPVSGGLSRTV
                     IAAAAGARSPLAGVLSAVALVPALLWATPLLGALPQAVLAATIVVPILKLVDPPGLLR
                     LWRYSRADAAALAATGAGVLLLGVELGLLAGLAVSIGAFLLQASRPHYAIVGRVPGTE
                     HYRNVLRHPVAGSQRVLTVRVDESLRFCNAAWLEDTVAQLVAGRPELTDVVLMCPGVN
                     AIDASAFESLERIDARLRAGGLRLHLSEVKGPVMDRLAAAGLPQRLSGQVFLSQHQAM
                     QALEGGR"
BASE COUNT          260 a          514 c          510 g          209 t
ORIGIN      
        1 gttcgcgacg acgccgacac atgagcaagt ctttcccagg tcccgggacg gcacatgaaa
       61 cagatcatta ttcgtgctca ggcagaagga ttccgccaga tcctgcgtcg aatacgcggc
      121 ggcctgagcg tcctgaagac ccgcaccagc cagaacgatt accatgggga tcgtcatcag
      181 cactttgttc atgtctgttt catcctgtat gccgggtgat gggcaaaact ttgttcctgg
      241 catcacgagc acgacgggga atatcgaccg gtccctggcc ccgtatgatc cggactgcac
      301 ttccgtaatc catcgcaacg ctcgtcggcc ggatggccgt aggccacaac gccatgttca
      361 tcggcgtcaa aacctctgac ggatggagga gctgcgactg gcagcattaa ttgttaccag
      421 atgtttcatc tggctgtggc cggagtaccg ccggaataca accggactcc ggacagcacg
      481 gatgcggacg gctgtgacag ccgatgcggc agggggtctg gaagacggac agatttgccg
      541 gcgctgccac tcacacgcgg gtcagcgatt tctgccgtgc aggccgggca tgccgctgcg
      601 ggcgcggcct gcgaagcagg ccatcagcgg gctgctgccg tcaccgcccg ccttccagcg
      661 cctgcatcgc ctgatgctgg ctcaggaaca cctgccccga caggcgctgc ggcaggccgg
      721 cggcggcgag gcggtccatg accgggccct tgacctcgga aagatgcagg cgcaggccgc
      781 cggcgcgcag gcgggcgtcg atgcgttcga ggctttcgaa ggcgctggcg tcgatggcgt
      841 tgacgcccgg gcacatcagc accacgtcgg tgagttccgg ccggccggcg acgagctgcg
      901 cgaccgtgtc ctccagccac gcggcattgc agaagcgcag gctctcgtcg acgcgcacgg
      961 tcagcacccg ctggctcccg gcgaccgggt ggcgcagcac gttgcggtag tgctcggtgc
     1021 cgggcacgcg gccgacgatc gcgtaatgcg gacggctcgc ctgcagcagg aaggcgccga
     1081 tcgacaccgc gagaccggcg agcaggccca gctcgacgcc gagcagcagc acgccggccc
     1141 cggtcgcggc cagggccgcc gcatcggcac gcgaatagcg ccacagccgc agcagccccg
     1201 gcgggtcgac gagcttcagg atcggcacga cgatggtcgc ggccagcacc gcctgcggca
     1261 gcgcgccgag cagcggcgtg gcccacagca gcgccggcac gagcgccacg gcgctcagca
     1321 cgccggccag cggcgaacgg gcaccggccg ccgccgcgat cacggtgcgc gacagcccgc
     1381 ccgaaaccgg atacgcaccg ctgaaggccg cggccagatt cgcgagcccg aggccgcgca
     1441 gttcctgcgc cggatcgatg cgctcgcgcc gcggcgctgc gaaggtctgc gcc
//
LOCUS       DAOQJH010000715         1486 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_82762_length_1486_cov_4.238295, whole genome shotgun sequence.
ACCESSION   DAOQJH010000715 DAOQJH010000000
VERSION     DAOQJH010000715.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1486)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1486)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1486
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82762_length_1486_cov_4.238295"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..856)
                     /locus_tag="PLQ18_13550"
     CDS             complement(<1..856)
                     /locus_tag="PLQ18_13550"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNE02726.1"
                     /translation="MNDETRNPRTVLIEKPADRHWNLPDHARARAHFDWAEVRAGLAG
                     LPGGGLNIGWEALDRHVADGNGARLALRWLGKDGAVRDYTYAQLLAEAQRFACVLERL
                     GVGPGECVAVLAGRIPELYIAALGALRAGIVLCPLFSAFGPEPVHTRLEKSHARVLVT
                     TVTLYQRKIAALRDTLPALDTVLTVGGDCPGTFRFEALMAQAETGCPVAATGPDSPAL
                     LHYTSGTTGSPKGALHVHGAVAAHAWSGRAVLDLRPGDVYWCTADPGWVTGISYGLIA
                     PLVNGVTMI"
     gene            1131..>1486
                     /locus_tag="PLQ18_13555"
     CDS             1131..>1486
                     /locus_tag="PLQ18_13555"
                     /inference="COORDINATES: protein motif:HMM:NF024773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HNE02727.1"
                     /translation="MTIRNLDAVFKPRSVAVIGRGSKDDAPDAQLVQNLIRQGFNGPI
                     MPVNPDRKALRGILCYRDLASLPLAPDLAVITTPMAGAPEQIRTLGELGTRAVLLLNE
                     EMLKSWDDGGTAIRQAL"
BASE COUNT          216 a          557 c          514 g          199 t
ORIGIN      
        1 tgatcatcgt cacgccgttg accagcggcg cgatcaggcc gtacgagatg ccggtgaccc
       61 agccggggtc ggccgtgcac cagtagacgt cgccggggcg caggtcgagc acggcccggc
      121 cgctccacgc atgggcggcc actgcaccgt gcacgtgcag cgcgcccttc ggcgaaccgg
      181 tcgtgccgct ggtgtaatgc agcagcgccg ggctgtcggg accggtcgcg gcgaccgggc
      241 agccggtctc ggcctgcgcc atcagcgcct cgaagcggaa ggtgccgggg cagtcgccgc
      301 cgacggtcag cacggtgtcg agcgcgggca gcgtgtcgcg cagcgccgcg atcttgcgct
      361 ggtacagcgt caccgtcgtc accagcacgc gcgcgtggct cttttcgagc cgggtatgca
      421 ccggctccgg cccgaaggcc gagaacagcg ggcagagcac gatgccggca cgcagcgccc
      481 cgagcgcggc gatgtagagc tccggaatgc gcccggccag caccgcgaca cactcgcccg
      541 gcccgacgcc gagccgctcc agcacgcagg cgaagcgctg cgcctcggcg agcagctgcg
      601 cataggtgta gtcgcgcacg gcgccgtcct tgccgagcca gcgcagcgcc agacgcgcgc
      661 cgtttccgtc ggccacgtgc cggtccagcg cctcccagcc gatgttgagc ccgcctccgg
      721 gcaggccggc gaggccggcg cgcacctctg cccagtcaaa gtgcgcgcgc gcccgggcgt
      781 gatcgggcag gttccagtgc cggtcggccg gcttctcgat caggaccgtc cgggggtttc
      841 gggtttcatc gttcacgctc gaaatcctcg accggtgacg caccttgcgg ctgcgccacc
      901 ggcagcatcg aaaatgggaa ggcggccttc acgccatgca gcccgcaagc ccccggcgtg
      961 gccccgccgc atgccgccgg ccgggcgggt gcatgttgat acctgtcaac cggcccggca
     1021 aacggctcga ctacactggc cggccaccgc tgcccgcccc ccggcccgac cgggtggcgc
     1081 tgcgcagcga tccgctccct gtccgtttcc tgccgctccg gaatcccgcg atgacgatcc
     1141 gcaacctcga cgccgtattc aagccccgtt ccgtcgccgt gatcggtcgc ggcagcaagg
     1201 acgatgcgcc cgatgcgcag ctcgtgcaga acctgatccg ccagggtttc aacgggccga
     1261 tcatgccggt gaacccggac cgcaaggcgc tgcgcggcat cctctgctac cgcgatctgg
     1321 ccagcctgcc gctcgcgccg gatctcgccg tgatcacgac gccgatggcc ggcgcgcccg
     1381 agcagatccg cacgctcggc gaactcggca cgcgcgccgt gctgctgctg aacgaggaga
     1441 tgctgaagag ctgggacgac ggcggcacgg cgatccgcca ggcgct
//
LOCUS       DAOQJH010000716         1483 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_83124_length_1483_cov_3.247199, whole genome shotgun sequence.
ACCESSION   DAOQJH010000716 DAOQJH010000000
VERSION     DAOQJH010000716.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1483)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1483)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1483
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83124_length_1483_cov_3.247199"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(91..804)
                     /gene="flgA"
                     /locus_tag="PLQ18_13560"
     CDS             complement(91..804)
                     /gene="flgA"
                     /locus_tag="PLQ18_13560"
                     /inference="COORDINATES: protein motif:HMM:TIGR03170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HNE02728.1"
                     /translation="MPADASTLFPTPLRRLLAAAVLALLSAAAPAAQQDLEALRLVAL
                     DYLRSRVGSSSADIRVDGTDPRLRLWACESPIEASLSPGSRLRGRTAVVLRCPAPAWK
                     LVMSAQVSIQRSIVVAARPLVRGTLLTAADLQVAAVDEARAGDGWIEDPKPAIGRLLQ
                     TALPAGAPLSPDALSDPVSVRRGETLELVVRRGAMRISAGGVALADGRPGERLRARNL
                     ASGRVVEGRLEQERTLIVD"
     gene            1032..1424
                     /gene="flgB"
                     /locus_tag="PLQ18_13565"
     CDS             1032..1424
                     /gene="flgB"
                     /locus_tag="PLQ18_13565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018953171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009429 - bacterial-type flagellum basal
                     body, proximal rod [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HNE02729.1"
                     /translation="MAIRFDRVLGVHEDAMRLRAQRTEVIAANLANADTPNYQAQDID
                     FAAAIRDAQGGARLAATAGAHIQPGEGRHGLAGTLFRESTQPSIDGNTVEPEREKAMF
                     GENAVQYTASLRLLGDRLQGLLTAIRGE"
BASE COUNT          212 a          519 c          543 g          209 t
ORIGIN      
        1 gtatacggaa ctccgtatcg gccccggccg ccggaacttg aatcgggttt tccggcagag
       61 cgttacgccc ggtgtcggtc gctccggggt tcagtcgacg atcagcgtgc gctcctgctc
      121 cagccggcct tcgacgacac gcccggaggc gaggttgcgc gcgcgcaggc gctcgccggg
      181 gcgaccgtcg gcgagtgcga ccccgccggc gctgatgcgc atcgcgccgc ggcgcacgac
      241 gagttccagg gtttcgccgc gacgcacgct gaccggatcg ctcagcgcat ccggtgacag
      301 cggggcgccg gccggcagcg ccgtctgcag caggcggccg atcgccggtt tcggatcctc
      361 gatccagcca tcgccggcgc gggcttcgtc gacggccgcc acctgcaggt cggccgccgt
      421 cagcagcgtg ccgcgcacca gcggccgggc ggcgacgacg atgctgcgct ggatgctgac
      481 ctgcgcgctc atcaccagct tccaggccgg ggccggacag cgcagcacga cggccgtgcg
      541 cccgcgcagt ctggagcccg gcgacagcga agcctcgatc ggcgattcgc aggcccacag
      601 ccgcaggcgc ggatcggtgc cgtcgacgcg gatgtcggcg ctgctgctgc cgacgcggct
      661 gcgcaggtag tcgagcgcga ccagacgcag ggcttcgaga tcctgctgcg cggccggggc
      721 tgcggcactg agcagggcga ggacggctgc ggccagcagc cggcgcagcg gggtcgggaa
      781 caaggtcgag gcatcggcag gcatggtggc gtcggctttt cgtcgtcgat cgacagatga
      841 atgccttcca gcacgcggta tgccagcccg ccgggttccc cgaccgccgg cgcctgtctg
      901 ccgtgcagga cggcggcggc gtgcggggct gctggatggc atgtcggttg cagatacccc
      961 tcccgagtcg cattcggcga cgagagtttg acgcgacgcc gctcgtggcg ctgcgtcggg
     1021 aggttgacga catggccatt cgtttcgacc gggtactcgg cgtgcacgag gacgcgatgc
     1081 ggctgcgtgc ccagcgtacc gaggtgatcg cggccaatct cgcgaacgcc gacaccccga
     1141 attaccaggc ccaggacatc gacttcgctg cggcgatccg cgacgcgcag ggcggtgcac
     1201 ggctcgcggc caccgccggc gcacacatcc agcccggcga gggccgccat ggcctggccg
     1261 gcacgctgtt tcgcgaatcg acgcagccga gcatcgacgg caacaccgtc gagccggagc
     1321 gcgagaaggc catgttcggc gagaacgccg tccagtacac ggcctcgctg cgcctgctcg
     1381 gcgaccggct gcagggcctg ctgaccgcga tccgcggcga atgaccggca ctcgggaggc
     1441 cacatgtcac tgttccgggt tttcgggatt tccggcagcg cgc
//
LOCUS       DAOQJH010000717         1481 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_83445_length_1481_cov_1.960729, whole genome shotgun sequence.
ACCESSION   DAOQJH010000717 DAOQJH010000000
VERSION     DAOQJH010000717.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1481)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1481)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1481
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83445_length_1481_cov_1.960729"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            60..1262
                     /locus_tag="PLQ18_13570"
     CDS             60..1262
                     /locus_tag="PLQ18_13570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HNE02730.1"
                     /translation="MPITDFQPLFPEERVLGPLREQAAALIAAAHRLAATQPALVAAL
                     QPLLRAMNSYYTNRIEGQHTLPADIERALHRQFDADVALARRQRLALAHMEAEAELEA
                     RVTAADPGGLYGAEFFASIHSALYARLPADDRFAGDGIEVLPGLWRDRRVSAGQHLAP
                     PPEEIPVLLEAWGQRYRGLPGGESRVIGALCAHHRLLWIHPFLDGNGRSARLHTHIVL
                     HALGLTQGLWSPMRGFARAQEQYYARLNNADLERRNDLDGRGSLSQEGLIAFVAWALE
                     VCLDQVRFIGERLHPGGFRERLQALLLALAARPWPVGSETSVVRPEALGAVHYVALNG
                     ALPRGEFLAMTGLPERTARRMLASLLDYGLLAADSPRAPVRFALPQKSLGLLFPRLWP
                     EAEQMAPD"
BASE COUNT          223 a          505 c          503 g          250 t
ORIGIN      
        1 aaaatcggca ttccgatctg tccatgaaac tgtccgcgcg tccgactgac ggggccgcca
       61 tgccgatcac cgactttcag cccctgttcc cggaggagcg cgtgctcggg ccgctgcggg
      121 agcaggccgc cgccctgatc gccgccgccc atcggctcgc cgccacccag ccggcactgg
      181 tcgcagcgct gcagccgctg ctgcgcgcga tgaactccta ttacacgaat cgcatcgagg
      241 ggcagcacac gctgccggcc gacatcgagc gcgcgctgca tcgtcagttc gatgcggatg
      301 tggcgctggc gcgcaggcag cgactggcgc tcgcgcacat ggaagccgag gccgagctgg
      361 aagcgcgggt cacggcagcg gatcccggcg ggctgtatgg agcggaattt ttcgcttcga
      421 tccacagcgc gctgtacgca cggctgcctg cagacgaccg ctttgccggc gatggcatcg
      481 aggtgctgcc ggggctctgg cgcgaccggc gggtaagcgc cggccagcat ctcgccccgc
      541 cgccggaaga gattccggtg ctgctcgaag cctgggggca gcgctatcgc ggactgccgg
      601 gcggcgagag ccgggtgatc ggcgcgctgt gcgcgcatca ccggctgctg tggattcatc
      661 cgttcctcga cggcaacggt cgcagtgcgc ggctgcatac gcacatcgtg ctgcacgcac
      721 tggggctgac gcagggcctc tggtccccga tgcgcggttt cgcgcgcgca caggagcagt
      781 actacgcgcg cctgaacaat gccgatctcg aacgccgcaa cgatctcgac ggccgcggtt
      841 ccctgtcaca ggaaggtctc atcgctttcg tcgcatgggc actcgaggtc tgtctggatc
      901 aggtgcgttt catcggcgag cggctgcatc ccggtggatt ccgggaacgg ctgcaggccc
      961 tgctgctggc gctggccgcc cgtccgtggc cggtcggatc ggaaacttcc gtggtcaggc
     1021 cggaagctct gggggccgtg cattacgtgg cactcaacgg agcactgccg cgcggagagt
     1081 ttctggcgat gaccggcctg ccggaacgca cggcccggcg catgctcgcc agcctgctgg
     1141 attacggatt gcttgcagca gacagcccgc gcgcaccggt gcgtttcgcg ctaccgcaaa
     1201 agtcactggg tctgctgttt ccgcgcctct ggcccgaagc cgaacagatg gcaccagact
     1261 gatcccatgc cgtgccggcc tgtcggctac acgcggatcg tcgtgtgact gcacggctta
     1321 caaggacttg cagaggcttc gcgcccgttg gcaagtgccg actctgcggc accgcagctc
     1381 tgcatttcac gcactcccgc atgcagttcc ggtctgtcat gttgaccgcg acgccgcgca
     1441 gcgcggtgca gatccgccgg cccgaagcgc aggccggccg g
//
LOCUS       DAOQJH010000718         1477 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_83850_length_1477_cov_2.960619, whole genome shotgun sequence.
ACCESSION   DAOQJH010000718 DAOQJH010000000
VERSION     DAOQJH010000718.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1477)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1477)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1477
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83850_length_1477_cov_2.960619"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1239)
                     /gene="rtcR"
                     /locus_tag="PLQ18_13575"
     CDS             complement(<1..1239)
                     /gene="rtcR"
                     /locus_tag="PLQ18_13575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019093717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA repair transcriptional activator RtcR"
                     /protein_id="HNE02731.1"
                     /translation="MSRNRPLVVIGLLGTTLDSGKGAGRWEKWRPSVALCQQPDRVID
                     RFELLYEVRFRELAEHVASDIGFISPETTLTLHALTMSDPWDFEEVYGALHDFARGYP
                     FAPEAEDYLVHITTGTHVAQICLFLLTEARELPARLLQTAPPRKQIASDPGSCAVIDL
                     DLSRYDRIARRFAREQVEQADFLKAGIATRNPAFNATIARIERVALRSRAPLLLLGPT
                     GAGKSFLAKRIYELKRARHRLSGAFVEVNCATLRGDGAMSALFGHKRGAYTGAQTERA
                     GLLRSADKGLLFLDEIGELGPDEQAMLLKAIEDGRFLPVGADKEVESRFELIAGTHRD
                     LAAEVAVGRFREDLYARINLWTFRLPGLAERREDIEPNLDFELERHAREQGQRVRFNA
                     EARRRYLDFATGPDAPWRGNF"
BASE COUNT          241 a          505 c          503 g          228 t
ORIGIN      
        1 gaagttgccg cgccagggcg cgtccgggcc ggtcgcgaag tcgaggtagc ggcggcgggc
       61 ttcagcgttg aagcgcacgc gctggccttg ctcgcgcgcg tggcgttcga gctcgaagtc
      121 gaggttcggc tcgatgtcct cgcgccgctc ggccagaccc ggcagccgga aggtccacag
      181 attgatccgt gcgtataaat cctcgcggaa gcgcccgacc gcgacctcgg cggccagatc
      241 gcgatgcgtg ccggcgatca gctcgaagcg gctttcgact tccttgtcgg cgccgaccgg
      301 caggaagcgg ccgtcctcga tcgccttcag cagcatcgcc tgctcgtccg gcccgagttc
      361 gccgatctcg tcgaggaaca gaagcccctt gtcggcactg cgcagcaggc cggcgcgttc
      421 ggtctgcgcg ccggtgtagg caccgcgctt gtggccgaac agcgccgaca tcgcgccatc
      481 gccgcgcagc gtcgcgcagt tgacctcgac gaaggcaccg ctcaaccggt ggcgggcacg
      541 cttgagttcg tagatgcgct tcgccagaaa gctcttgccg gcgccggtcg gcccgagcag
      601 cagcagcggc gcgcgcgagc gcagcgcgac gcgctcgatg cgcgcgatcg tcgcgttgaa
      661 ggccggattg cgggtggcga tgccggcttt caggaaatcc gcctgctcga cctgctcgcg
      721 cgcgaagcgc cgggcgatgc ggtcgtagcg cgacaggtcg aggtcgatca ccgcgcagct
      781 gccgggatcg ctggcgatct gcttgcgcgg cggcgccgtc tgcagcagcc gcgccggcag
      841 ctcgcgcgct tcggtcagca ggaacaggca gatctgcgcg acgtgcgtgc cggtggtgat
      901 gtgcacgagg taatcctcgg cctcgggcgc gaacggatag ccgcgcgcga agtcgtgcag
      961 cgcgccgtag acctcctcga aatcccacgg atcgctcatc gtcagcgcat gcagcgtcag
     1021 cgtcgtctcg ggcgagatga aaccgatgtc gctggcgaca tgctcggcca gttcgcgaaa
     1081 gcgcacctcg tacagcagct cgaagcggtc gatcacgcga tccggctgct gacacagcgc
     1141 cacgctcggc cgccatttct cccagcgccc ggcgcccttg ccgctgtcga gcgtggtgcc
     1201 gagcagaccg atgacgacca gcggacggtt ccgggacatg aggcaggctt ccgggggaca
     1261 ggagccatca ggataaatcc tgatccgtac ggatcagaaa tcggctcgct accgaaccca
     1321 tgcctgcagc ccgccgtgcg cacaaattca ttccggatca atcttctgcc gcgcgatgta
     1381 cgcatctggc acgctgcctg cgataccgga gccatccccc gacgccacga ctgccggcgc
     1441 aacgatccgg cgaggagtcc cgcgatgagc agcaaga
//
LOCUS       DAOQJH010000719         1470 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_84646_length_1470_cov_3.437456, whole genome shotgun sequence.
ACCESSION   DAOQJH010000719 DAOQJH010000000
VERSION     DAOQJH010000719.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1470)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1470)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1470
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84646_length_1470_cov_3.437456"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..598
                     /locus_tag="PLQ18_13580"
     CDS             <1..598
                     /locus_tag="PLQ18_13580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02732.1"
                     /translation="ELSQPLATIASYAEACHLMLTKPGSEPLMAATLTAIVAQASLAG
                     QLLQALRQALHGTGTPAPPAGDDAPTLAALLARLAPVLATHAETQVMTPAVSLWRTAA
                     AVAAGLDAELPTLAARLHAVSDQPCHLLAALAPEGGARLYLMPAPAAAASITGQHRLL
                     DTDTGDCAQRLLGCGISLSPHWQADRGLCFELRLPGPD"
     gene            complement(643..>1470)
                     /locus_tag="PLQ18_13585"
     CDS             complement(643..>1470)
                     /locus_tag="PLQ18_13585"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HNE02733.1"
                     /translation="YLPPSSPLALVGDRVRLVQAVGNLLHNASRYSPAGARIELMAER
                     SGSEAVIRVRDNGRGIAPERLAQLFRLFVHSAPDDPGDSPSEPGSGDDSLSFGLGLVL
                     ARRLIELHGGTLTAASPGPGCGSTFTIRLPLDAPADVRPLIAGTAVLPEPLPVVPRRV
                     LLVDDNNDVRTALAALLEAMGHEVCALSDGHEVSDAVGSFGPHTVLLDLAMPKMNGYA
                     VARALRERYPDAVLQLVALTGFGGPDEQQRIREAGFDRHLLKPVSREQLAALLAGGG"
BASE COUNT          193 a          559 c          541 g          177 t
ORIGIN      
        1 cgaactgagc cagccgctcg ccacgatcgc gagctacgcc gaagcctgtc atctgatgct
       61 gacgaaaccc ggcagcgaac ccctgatggc cgcgaccctg acggcgatcg tggcgcaggc
      121 cagcctggcc ggacagctgc tgcaggcgct gcgtcaggcg ctgcacggca ccggcacgcc
      181 ggccccgccg gccggggatg acgcgccgac gctcgccgcc ctgctggcgc ggctcgcgcc
      241 ggtgctggcc acgcacgccg aaacgcaggt catgacgccc gccgtcagcc tgtggcgcac
      301 ggccgcggcg gtcgcggccg gtctcgatgc cgaactgccg acgctggctg cccgcctgca
      361 cgccgtcagc gatcagccct gccatctgct cgcggcactg gcgcccgaag gcggcgcccg
      421 gctgtacctg atgcctgcgc cggccgcggc ggcgtcgatc accggccagc accggctgct
      481 cgacaccgac acgggcgact gcgcgcagcg actgctgggc tgcggcatca gcctgagccc
      541 gcactggcag gccgaccgcg gcctgtgctt cgaactgcgc ctgccgggcc cggactgaac
      601 gcaccgggcc gccggagcgc cacgccccgc aagcggcccg gctcagcccc ctccggcgag
      661 cagggccgcg agctgttcgc ggctcacggg cttcagcagg tggcggtcga acccggcctc
      721 gcggatgcgc tgctgttcgt ccgggccgcc gaagccggtc agcgcgacca gttgcagcac
      781 ggcgtccgga taccgctcgc gcagcgcgcg cgccacggcg tagccgttca tcttcggcat
      841 cgccagatcg agcagcaccg tgtgcggccc gaagcttccg acggcgtccg acacctcgtg
      901 cccgtccgag agcgcgcaga cctcgtgccc catcgcttcg agcagcgccg cgagggcggt
      961 gcgcacgtcg ttgttgtcat cgacgagcag cacgcgccgc ggcacgaccg gcagcggctc
     1021 cggcagcact gccgtgccgg cgatgagggg ccggacatcg gccggggcat cgagcggcag
     1081 gcggatcgtg aacgtgctgc cgcagccggg gccgggactc gccgcggtca gcgtcccgcc
     1141 gtgcagctcg atgagccggc gcgcgagcac gagtccgagg ccgaagctga ggctgtcgtc
     1201 gccggagccg ggctcggagg ggctgtcgcc gggatcgtcc ggggcgctgt gcacgaacag
     1261 ccggaacagc tgcgccagcc gctcgggggc gatgccgcgg ccgttgtcgc gcacgcggat
     1321 cacggcctcg ctgccgctgc gctcggccat cagctcgatg cgggccccgg ccgggctgta
     1381 gcggctggcg ttgtgcagca ggttgccgac cgcctgcacc agccgcaccc ggtcgccgac
     1441 gagcgcgagc gggctggacg gcggcaggta
//
LOCUS       DAOQJH010000720         1461 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_85722_length_1461_cov_2.854908, whole genome shotgun sequence.
ACCESSION   DAOQJH010000720 DAOQJH010000000
VERSION     DAOQJH010000720.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1461)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1461)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1461
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85722_length_1461_cov_2.854908"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..466)
                     /locus_tag="PLQ18_13590"
     CDS             complement(<1..466)
                     /locus_tag="PLQ18_13590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4276 family protein"
                     /protein_id="HNE02734.1"
                     /translation="MIRVACIVEGDGEVTALPVLLRRLTDRLAPGHWIDIPPPIRVKR
                     DRFLNRPEEFSRILQLAALKAGEDGRVLILLDADDACPAQLGRDLQHRANTGIPHRPV
                     SVVLANREYEAWFLAACASLDGQRGLHIPPDPPRQPENIRGAKEWLGRHMHGG"
     gene            complement(463..>1461)
                     /locus_tag="PLQ18_13595"
     CDS             complement(463..>1461)
                     /locus_tag="PLQ18_13595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007195517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNE02735.1"
                     /translation="EVRRKSNGHPTHFGIRLEFMLRDGGTGYYAFEVGALAGGSYEVR
                     HEECSLRGFGNGPYFRLERGQLQAGSEASFPAVTSDRLALVSASGLAAFRPVYDALTA
                     MGFYNLNPKLIRELQKPQDGRLLKPDGGNIASVIGQMTRHAPEALATVAEYLKTVVPG
                     VHAVQRVAVGPMESLEFRQDMAGARNPWKFPALNMSDGTLRTLGVLTALFQGDPAAPP
                     TVIGIEEPETALHPAAAAALREALCVASTRTQIIVTSHSPELLDDPDLPAGSLYAVVN
                     EAGESKIAPIDPASRQALTEHLFTAGELLRMNQLAPDPDAMTSRIDQMELFGERCE"
BASE COUNT          235 a          461 c          495 g          270 t
ORIGIN      
        1 gtccgccgtg catgtgccgg ccgagccact ccttggcgcc gcggatgttt tcgggctgac
       61 gcggcggatc gggcgggatg tgcaaaccgc gctggccgtc gagcgaggcg caggcggcga
      121 gaaaccaggc ctcgtactcg cgattggcca ggacgacgct gaccgggcga tggggaattc
      181 cggtgtttgc ccggtgctgc aggtcgcggc ccagttgcgc cggacaggca tcatcggcat
      241 cgagcaggat cagcacccgt ccgtcctcgc cggctttcag cgcagcgagt tgcaggattc
      301 gggagaattc ttcgggccgg ttcagaaaac ggtcacgctt gacgcggatc ggtggcggaa
      361 tgtcgatcca gtgtcccgga gccagccggt ccgtcagtct gcgcagcagc accggcaatg
      421 ccgtcacctc gccgtcgcct tcgacgatgc aggccaccct gatcactcgc agcgctctcc
      481 gaacagttcc atctggtcga tgcggctggt catggcgtcc ggatcggggg caagctgatt
      541 catccgcaac agttcgccag ccgtgaatag gtgttcggtc aatgcctgac ggcttgccgg
      601 gtcgatcggg gcaatcttcg attcgccggc ttcgttgacg acggcataca gggagccggc
      661 gggcaggtcc ggatcgtcga gcagttccgg actgtggctg gtgacgatga tctgcgtgcg
      721 ggtgcttgcc acgcacaggg cttcgcgcag tgctgcggct gcggcgggat gcagggcggt
      781 ttcgggttcc tcgatgccga tgaccgtcgg cggggcggcc ggatcgccct ggaacagcgc
      841 ggtcaggacc cccagcgtgc gcagcgtgcc atccgacatg ttcagggccg gaaacttcca
      901 cggattgcgc gcgcctgcca tgtcctgacg gaattccagc gattccatcg gtccgaccgc
      961 tacgcgctgc actgcgtgga cgccgggcac gacggttttc agatattcgg ccacggtggc
     1021 gagggcttcg ggtgcatggc gggtcatctg gccgatgacg ctggcgatgt tgccgccatc
     1081 gggcttcagc agccggccgt cctgtggttt ctgcagttcc cggatcagtt tcggattcag
     1141 gttgtagaaa cccattgccg tcagggcatc gtaaaccggc cggaacgccg caagccccga
     1201 agcgctcacc aatgccagcc gatcgctggt aacggccgga aagcttgcct cgctgcctgc
     1261 ctgcaactgg ccgcgctcca gccggaaata cggcccgttt ccgaaaccgc gcagcgaaca
     1321 ttcctcgtga cggacttcgt agctgccgcc ggccagggca ccgacttcga atgcgtagta
     1381 gccggtgccg ccatcgcgca gcatgaattc cagccggata ccgaaatgcg tcgggtgacc
     1441 gttcgacttg cggcggactt c
//
LOCUS       DAOQJH010000721         1453 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_86717_length_1453_cov_4.201001, whole genome shotgun sequence.
ACCESSION   DAOQJH010000721 DAOQJH010000000
VERSION     DAOQJH010000721.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1453)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1453)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1453
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86717_length_1453_cov_4.201001"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..419
                     /locus_tag="PLQ18_13600"
     CDS             <1..419
                     /locus_tag="PLQ18_13600"
                     /inference="COORDINATES: protein motif:HMM:NF019000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0042262 - DNA protection [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HNE02736.1"
                     /translation="QRLEAAMNEELAAVRARHEAEAAPHTAAIDGLSHAVEVWADANR
                     ALLTRHGRTRSAKLASGDIGWRLSPPAVEVEGEAAVLEELQRRGLQRFVRVRESLNRE
                     AVLHEPEAVADVPGIEVVQHEAFVIKPYATPLDESA"
     gene            542..1321
                     /locus_tag="PLQ18_13605"
     CDS             542..1321
                     /locus_tag="PLQ18_13605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017008488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HNE02737.1"
                     /translation="MLDLFSADPSLLLMFAVVSFTAGFVDAIAGGGGLLTVPALLSAG
                     IPPHLALGTNKLSATFGSSTASFAFVRKGLFRPADWRAAALATLIGAGFGALLTRAFS
                     ADVLKTLLPLSVLGAALYLVWPRRRLRRGEPDPAFRASGRSQWTAGLGIGFYDGFIGP
                     GTGAFWMAVAVKYFRQDLVRGAGLARFMNFLSNAAALLMFALLGNIDWALGLAMGPAL
                     MLGAWLGAHSAIRYGSPLIRPMFIAVVVAMTIRLLWVQFAH"
BASE COUNT          182 a          533 c          517 g          221 t
ORIGIN      
        1 gccagcgcct cgaagcggcg atgaacgagg aactcgccgc cgtgcgcgcc cgccacgagg
       61 ccgaggccgc gccgcacacc gccgcgatcg acggcctgag ccacgccgtc gaggtctggg
      121 ccgatgccaa ccgcgcactg ctgacccgcc acggcaggac gcgcagcgcg aagctcgcga
      181 gcggcgacat cggctggcgc ctgagcccgc cggccgtcga ggtcgagggc gaggccgcgg
      241 tgctcgaaga actgcagcgg cgcggcctgc agcgcttcgt gcgcgtgcgc gagagcctga
      301 acagggaagc cgtgctgcac gagcccgaag cggtcgccga cgtgcccggc atcgaggtcg
      361 tgcagcacga ggccttcgtc atcaagccct acgcgacccc gctcgacgag tccgcgtaaa
      421 cccggccggc cgcggccggc accgggcttc ggcagcggcc tccccgaagc gttataaagc
      481 cggcccgcag ccgccggctt cccgtccggc gcgcgccctc tcccgctgcc cggtattcgt
      541 catcttggat ctcttctcgg ccgatccgtc gctgctgctg atgttcgccg tcgtgtcgtt
      601 cacggccggc tttgtcgatg ccatcgccgg cggcggcggc ctgctgaccg tgccggcgct
      661 gctgtcggcc ggcatcccgc cgcatctggc gctcggcacc aacaagctca gcgcgacctt
      721 cggcagctcg accgcctcct tcgccttcgt gcgcaagggc ctgttccggc ccgcagactg
      781 gcgtgccgcg gcgctcgcga cgctgatcgg cgccggcttc ggcgcgctgc tgacgcgcgc
      841 cttcagcgcc gacgtgctga agacactgct gccgctgtcg gtgctcggcg cggcgctgta
      901 tctggtctgg ccgcgacgcc gcctgcggcg cggggaaccg gacccggcct ttcgcgcctc
      961 cgggcgctcg cagtggacag cgggcctcgg catcggcttc tacgacggct tcatcggccc
     1021 cggcaccggc gcgttctgga tggcggtggc ggtgaagtac ttccgccagg acctggtgcg
     1081 cggcgccggg ctggcgcgct tcatgaactt cctgagcaac gccgcggcgc tgctgatgtt
     1141 tgcgctgctc ggcaacatcg actgggcgct cgggctggcg atgggcccgg cgctgatgct
     1201 cggcgcctgg ctcggcgcac acagcgcgat ccgctacggc agcccgctga tccgcccgat
     1261 gttcatcgcc gtcgtcgtcg cgatgacgat ccggctgctg tgggtgcagt tcgcgcactg
     1321 agcacgtgtg gccctgctgc accggagcgg gagtgccgca ttcgcgggca ggacgaaggc
     1381 ggtatcgatg ccgcgcgtgg cagcaaccgc aggctgggtt gacgcaggaa acccggcgat
     1441 tccggcgatg acg
//
LOCUS       DAOQJH010000722         1430 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_89420_length_1430_cov_5.365091, whole genome shotgun sequence.
ACCESSION   DAOQJH010000722 DAOQJH010000000
VERSION     DAOQJH010000722.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1430
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89420_length_1430_cov_5.365091"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /locus_tag="PLQ18_13610"
     CDS             complement(<1..251)
                     /locus_tag="PLQ18_13610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02738.1"
                     /translation="MPAPLLACGPWRLLRFLQDQPLIWPSLLSADEIAARLEAGPCSS
                     RTGPEWTLIVPADRPLGADLGLEARQRLVRFSERLSCATL"
     gene            complement(694..1353)
                     /locus_tag="PLQ18_13615"
     CDS             complement(694..1353)
                     /locus_tag="PLQ18_13615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018716917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HNE02739.1"
                     /translation="MQRIAVYCGSASGSDPRFAEAATAFGALLAARGLELVFGGGHVG
                     LMGRVADAVLAGGGRAIGVMPDLLAEREIAHRGLTELQIVPDLQQRKRRMLELADACI
                     ALPGGIGTFDEWFEALTLGCLELHDRPCALLNLHGYHDPLLALLDHAVAQGFMRPQQR
                     AAVLVDDDGARLLDACAARRATQACNDDPIGQDAEQRTKKTRSYRQGEGGDRVIGGSD
                     I"
BASE COUNT          243 a          488 c          478 g          221 t
ORIGIN      
        1 agcgtcgcgc agctcagccg ctcgctgaag cgcacgaggc gctggcgcgc ctccagcccc
       61 agatcggcac cgagcggccg gtcggcgggc acgatcagcg tccactccgg gccggtgcgg
      121 ctgctgcacg gtccggcttc gaggcgggcg gcgatctcgt cggcgctcag cagcgagggc
      181 cagatcagcg gctggtcttg caggaagcgc agcagtcgcc acgggccaca ggccagcagc
      241 ggcgccggca tggcgagtcc ggacaggcag acgagagaag gcatgggtgg tcgacttgcg
      301 gcacgcgggc cgggttggac gtgaaccgta agcgtcggcc acgtccgttc gaccacccgc
      361 aggccggtct acgcacggat caacgccagc gtgccttgtc gatccatcgg tcgggacttg
      421 attgaagacc tggactttgc ccaggccgga attacatcgg cttcgatccg taccggccgt
      481 catgctgcgc ctgcacagca cgggctcatg aaaatacatt gtggtggctg tggtaatacg
      541 tcagtcgccg tatatagcga catccgtcga aacggtcgct tgacccggat atgtaagcat
      601 agctgacacc cctgttgcac cggtaaaaaa agtcgggtcc gactgatacc agagcgctgc
      661 actagggtat tctgcgtcgc gacatccctc gattcagatg tcgctccccc caattacgcg
      721 gtcgccgcct tccccctgac ggtacgaccg cgtttttttc gttcgctgct ccgcgtcctg
      781 cccgatggga tcgtcgttgc acgcctgcgt cgcccgccgg gccgcgcagg cgtcgagcag
      841 ccgagcgcca tcatcatcga cgagcaccgc cgcgcgctgc tgcggacgca tgaagccctg
      901 cgcgacggcg tgatcgagca gcgccagcag cggatcgtga tagccgtgca ggttcagcag
      961 cgcgcagggc cggtcgtgca gttcgagaca gccgagcgtc agcgcctcga accattcgtc
     1021 gaaggtgccg ataccgccgg gcagcgcgat gcaggcatcg gcgagttcca gcatgcggcg
     1081 cttgcgctgc tgcaggtcgg gcacgatctg cagttcggtg agaccgcggt gtgcgatctc
     1141 gcgctcggcc agcaaatccg gcatcacgcc gatcgcgcgc ccgccgccgg caagcacggc
     1201 atcggcaacc cgccccatca gcccgacgtg gccgccgccg aacacgagct ccagcccgcg
     1261 cgccgcgagc agcgcaccga aggcggtcgc ggcttcggcg aaacgcggat cgctgccgct
     1321 ggcggaaccg caatagacgg cgatgcgctg catgacgtgg cctccacggc gtcggagcgg
     1381 ccaccgtagc gcaggactcg ctaccagtcg ccgtccggca gcggtttcag
//
LOCUS       DAOQJH010000723         1430 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_89441_length_1430_cov_3.203636, whole genome shotgun sequence.
ACCESSION   DAOQJH010000723 DAOQJH010000000
VERSION     DAOQJH010000723.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1430
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89441_length_1430_cov_3.203636"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            25..603
                     /locus_tag="PLQ18_13620"
     CDS             25..603
                     /locus_tag="PLQ18_13620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012113221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HNE02740.1"
                     /translation="MPIHPHRRSLPAVLAVLLASLAAPGHAAPPAAPTAVAMAADTRP
                     TNTTVLPLALAEAAARAAIERCEADGHHVSAAVVDFSGLERAYLRGDHATVHTHETAF
                     KKAYTIVTLGPIFGADTTGQLTEKLSKSPTAPALASVGNVILLAGGVLIRAGDEPLAA
                     IGVGGAPGGALDEACAQAGAAAIQARVEALRR"
     gene            662..964
                     /locus_tag="PLQ18_13625"
     CDS             662..964
                     /locus_tag="PLQ18_13625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HNE02741.1"
                     /translation="MTTMHLLTKPKLPDPLELPTFLSNQDFVADPAKACRAAQRGPVV
                     IIENDRPTYVLMRVDDELEMSTADANLADLLAMPEADPIEFDPPRADGLWRPADLS"
     gene            973..1386
                     /locus_tag="PLQ18_13630"
     CDS             973..1386
                     /locus_tag="PLQ18_13630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019641128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNE02742.1"
                     /translation="MYLLDTNIVSELRKGGRADANVRIWASQQPLSRQFLSAITVLEL
                     ETGILQKERRDPVQGGLLRRWLEQQVLPRFENRILPVDLEIARRCACLHVPDPRSDRD
                     ALIAATALVHDFVVVTRNVPDFAPTGVRIFNPWQD"
BASE COUNT          244 a          502 c          442 g          242 t
ORIGIN      
        1 tgtcgactgt ccgaggaatc cgccatgccg attcatccgc accgccgttc cctgcctgcc
       61 gtccttgccg tgctgctcgc gagcctcgcc gcacctggtc acgccgcccc gccagcggca
      121 ccgaccgctg tcgcgatggc cgcggacacc cggccgacga acaccaccgt actgccgctc
      181 gccctcgccg aggccgctgc gcgcgccgcg atcgaacgtt gcgaggctga cggccatcac
      241 gtttcggccg ccgtcgtcga tttttccggg ctggaacgcg cctacctgcg tggcgatcac
      301 gcgaccgtgc acacgcacga aaccgcgttc aaaaaggcct acaccatcgt cacgctcggc
      361 ccgatcttcg gcgccgacac caccggccag ctcaccgaga agctgtcgaa gtcaccgacc
      421 gcaccggcgc tcgccagcgt cggcaacgtc atcctgctcg cgggcggcgt gctgatccgc
      481 gccggcgacg aaccgctggc tgcaatcggc gtcggcggcg ccccgggcgg tgcgctcgac
      541 gaagcctgcg cgcaggccgg ggcggcggcg atccaggcgc gggtggaggc gctgcggcgc
      601 tgacgacgta cccggaagct tgggtgtaag gtgtcacgaa gcactggaag gagagacagt
      661 cgtgacaacg atgcatcttc tgacgaaacc caagcttccc gatccgctgg agttaccgac
      721 tttcctgtcg aatcaggatt tcgtcgccga tcccgcgaag gcgtgtcgtg ccgcgcagcg
      781 agggcctgtc gtcattatcg agaacgacag accgacgtat gtgctgatgc gcgtggacga
      841 tgagctggag atgtcgacag cggacgcgaa cctagcggat ctgctggcga tgccggaggc
      901 ggacccgatc gaattcgatc cgccgcgggc ggatggactc tggcgtcctg ccgacctgtc
      961 ctgagactgt ccgtgtatct gctcgatacc aacatcgttt ccgaactgcg caagggcggc
     1021 cgtgcggatg cgaatgtcag gatctgggca agtcagcagc cattatcccg acagttcctt
     1081 tccgcgatca cggtgctcga actcgaaacc ggcatcctgc agaaggaacg ccgtgatccg
     1141 gtacaaggcg ggctgctgcg ccgctggctg gaacagcagg tactgccgcg tttcgaaaac
     1201 cgcatcctgc ccgtcgatct cgagatcgcc cgtcgctgtg cttgcctgca tgtacccgat
     1261 ccacgctcgg accgtgatgc cctgatcgca gcaaccgcgc tcgtccacga cttcgtggtc
     1321 gtcacccgca acgtccccga cttcgcaccg accggcgtcc gcatcttcaa tccctggcag
     1381 gactgaatga ctgccctgca ccggcagggc tgccaccgcc ctcgatgtca
//
LOCUS       DAOQJH010000724         1426 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_89904_length_1426_cov_2.929249, whole genome shotgun sequence.
ACCESSION   DAOQJH010000724 DAOQJH010000000
VERSION     DAOQJH010000724.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1426
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89904_length_1426_cov_2.929249"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="PLQ18_13635"
     CDS             <1..213
                     /locus_tag="PLQ18_13635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02743.1"
                     /translation="LHAMAGLGTLMGAVFAGLYFGVFRPLRAAAAAGERERAVRLAGR
                     LRKLVLLNLGFGTLVVLAAALARYGH"
     gene            286..921
                     /gene="rsmG"
                     /locus_tag="PLQ18_13640"
     CDS             286..921
                     /gene="rsmG"
                     /locus_tag="PLQ18_13640"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HNE02744.1"
                     /translation="MKLEDALADGLGRLGLCPPSPAITALAAYLRLLEQWNRAYNLTA
                     VRELGDMVAKHVLDSAAVLPWLHGARVLDVGSGAGLPGIPLAILSPERDYVLLDGLGK
                     RTRFLTQVVLELKLAHVQVVQARVEDWQAPAPFTSVISRAFAALPEFVELAGPHCASD
                     GRLLAMKGQLDAAETAAVPPGWRLRATHRLDVPGINGERHLLELEPLAAAA"
     gene            947..>1426
                     /locus_tag="PLQ18_13645"
     CDS             947..>1426
                     /locus_tag="PLQ18_13645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007924494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNE02745.1"
                     /translation="MGKVIAIANQKGGVGKTTTAVNLAAALAAAKARLKIILIDLDPQ
                     GNATMGCGIDKHGAEATMTEVLLGETSIGEALVYSESAGIQVLPSNGDLTAAEIGLAT
                     REQGAFRLRETLAAIRGNFDYIVIDCPPSLNMLTVNALTAAHSILIPVQCEYYALEGL
                     "
BASE COUNT          194 a          532 c          488 g          212 t
ORIGIN      
        1 ctgcacgcga tggccgggct cggcacgctg atgggcgccg tgttcgccgg gctttatttc
       61 ggcgtgttcc ggccgctgcg cgccgcggcc gccgccggcg agcgcgagcg ggccgtccgg
      121 ctcgccggcc ggctgcgcaa gctcgtgctg ctcaacctcg gcttcggcac gctggtcgtg
      181 ctggccgcgg cgctggcccg ctacggtcac tgagccgtcg ccggccgccc gcggcggccg
      241 tgatacactc cgccggctcg tccgctgccg tccccgatcg cccgcatgaa gctcgaagac
      301 gctctcgctg acggcctcgg ccgcctcggc ctgtgcccgc cgtcgccggc catcaccgca
      361 ctcgccgcct atctgcgcct gctcgaacag tggaaccgcg cctataacct gaccgccgtg
      421 cgcgagcttg gcgacatggt ggcgaagcac gtgctcgaca gcgccgcggt gctgccgtgg
      481 ctgcacggtg cgcgcgtgct cgatgtcggc agcggcgccg gcctgcccgg catcccgctg
      541 gcgatcctga gcccggagcg cgactacgtg ctgctcgacg gactcggcaa gcgcacgcgc
      601 ttcctgacgc aggtggtgct ggagctgaag ctcgcgcacg tgcaggtcgt gcaggcgcgc
      661 gtcgaggact ggcaggcacc ggcgccgttc acgagcgtga tctcgcgggc cttcgcggcc
      721 ctgccggagt tcgtcgaact cgccgggccg cactgcgcga gcgacggccg gctgctggcg
      781 atgaagggcc agctcgacgc tgccgagacc gccgcggttc cgcccggctg gcggttgcgg
      841 gcgacacacc ggctcgacgt gccgggcatc aacggcgagc gtcatctgct cgaactcgaa
      901 ccgctcgctg cggccgcctg aattcttcat caccggtgat tcacgcatgg gcaaggtcat
      961 cgccatcgcg aaccagaagg gcggggtcgg caagaccacc accgccgtca acctcgccgc
     1021 cgccctcgcc gccgccaagg cgcggctgaa gatcatcctg atcgacctcg atccgcaggg
     1081 taacgccacc atgggctgcg gcatcgacaa gcacggcgcc gaggcgacga tgaccgaggt
     1141 gctgctcggc gagacctcga tcggcgaggc gctggtgtac tcggaatcgg ccggcatcca
     1201 ggtgctgccg tccaacggcg acctgaccgc cgccgagatc ggcctcgcga cccgcgagca
     1261 gggcgcgttc cgcctgcgcg agacgctcgc ggcgatccgc ggcaacttcg attacatcgt
     1321 catcgactgc ccgccgtcgc tgaacatgct gacggtcaac gccctgaccg ccgcccacag
     1381 catcctgatc ccggtgcagt gcgagtacta cgcgctcgaa ggcctg
//
LOCUS       DAOQJH010000725         1422 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_90362_length_1422_cov_4.174835, whole genome shotgun sequence.
ACCESSION   DAOQJH010000725 DAOQJH010000000
VERSION     DAOQJH010000725.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1422)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1422)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1422
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90362_length_1422_cov_4.174835"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..357)
                     /locus_tag="PLQ18_13650"
     CDS             complement(<1..357)
                     /locus_tag="PLQ18_13650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020202982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNE02746.1"
                     /translation="MTDSTVAVIGAGPAGLMAAEVLATAGCTVEVHEAMPSPARKLLR
                     AGVGGLNLTHGEPPAQFLSRYGDRQPEIAPLLAGFDAAALRAWVHGLGIDTFVGSSGR
                     VFPEGMKAAPLLRAWLQ"
     gene            521..1063
                     /locus_tag="PLQ18_13655"
     CDS             521..1063
                     /locus_tag="PLQ18_13655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02747.1"
                     /translation="MNLKLTKPKLFAERSLSKVAGVFDDADTARVAARRARNEGNMVL
                     PQVQIVAPGDPQLSARIEPEQERIPETLLRSHLVCGTAGAGLGLLLAFALALSGVAAV
                     TSNLTASLMLLPLFGGLFGLLIGGAIALRPDIGRVAMTVRKETQSGRWVVVAHPVNAD
                     QQHGAERVFQDLRARTVSSL"
     gene            complement(1116..>1422)
                     /locus_tag="PLQ18_13660"
     CDS             complement(1116..>1422)
                     /locus_tag="PLQ18_13660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02748.1"
                     /translation="TFAPEVIFAAPKLTALFAGIGRDGPLAPVERAKGVLRTGRDWRL
                     FDAVAGGGLTVSDVSWRRDSRIECIAFEDARPDWTAIGAAWLAARGEAGAEGGQQGG"
BASE COUNT          200 a          528 c          483 g          211 t
ORIGIN      
        1 ctgcagccag gcgcgcagca gcggcgcggc cttcatgccc tcggggaaca cccggcccga
       61 actgccgacg aaggtgtcga tgccgagtcc gtgcacccag gcccgcagcg ccgcggcatc
      121 gaagccggcg agcagcggcg cgatttcggg ctggcgatcg ccgtagcgcg acaggaactg
      181 cgccggcggc tcgccgtgcg tcaggttcag gccgccgacc ccggcgcgca gcagcttgcg
      241 cgccggcgac ggcatcgcct cgtggacctc gaccgtgcag ccggccgtcg ccagcacctc
      301 ggccgccatc agtccggccg gcccggcccc gatcacggcc accgtcgaat ccgtcaagtc
      361 ccgccctcct ccggcgtctg cgtccggcca ttctgcagct tcgtgccagc cttgctgaac
      421 cttgcggaac tgtcatatgc ccggccggtc gcaatggcgg ggtcgaaggc cgcttccgca
      481 tgaggcggga gcttcatgga accgatcaag gagaaagccg atgaacctca agctgacgaa
      541 accgaagctg tttgccgagc gttcgctgag caaggtggcc ggcgtctttg acgatgccga
      601 caccgcgcgc gtcgccgccc ggcgtgcccg taacgaaggc aacatggtgc tgccgcaggt
      661 gcagatcgtc gctcccggcg atccgcagct gagcgcccgc atcgaacccg agcaggagcg
      721 catccccgaa accctgctgc gctcgcacct ggtgtgcggc acggccggag cggggctggg
      781 gctgctgctc gcgttcgcgc tggcgctgtc cggcgtggcg gccgtcacgt ccaacctgac
      841 ggccagcctg atgctgctgc cgctgttcgg tggcctgttc gggctgctga tcggcggcgc
      901 gattgccttg cggcccgaca tcggtcgtgt ggcgatgacc gtgcgcaagg aaacccagtc
      961 gggccgctgg gtggtcgttg cgcatccggt caacgcggac cagcagcatg gcgccgagcg
     1021 cgtgttccag gacctgcggg cgcggacggt cagttcgctc tgatccggac cgtttccagg
     1081 cgcagccggc tcgatcgccc ggctgcgcct tttcattacc cgccctgttg tccgccctca
     1141 gcgcccgcct ccccccgcgc cgccagccag gccgcgccga tcgccgtcca gtccgggcgc
     1201 gcgtcctcga aggcgatgca ttcgatgcgg ctgtcacggc gccagctcac gtcgctgacc
     1261 gtcaggccgc cgccggcgac cgcatcgaac agccgccagt cgcgcccggt gcgcagcacg
     1321 cccttggcgc gctcgaccgg ggccagcgga ccgtcacgac cgatgccggc gaacagtgcc
     1381 gtgagcttcg gtgccgcaaa gatcacctcg ggcgcaaacg tc
//
LOCUS       DAOQJH010000726         1414 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_91375_length_1414_cov_4.412068, whole genome shotgun sequence.
ACCESSION   DAOQJH010000726 DAOQJH010000000
VERSION     DAOQJH010000726.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1414
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91375_length_1414_cov_4.412068"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(165..>1414)
                     /locus_tag="PLQ18_13665"
     CDS             complement(165..>1414)
                     /locus_tag="PLQ18_13665"
                     /inference="COORDINATES: protein motif:HMM:NF037707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas3''"
                     /protein_id="HNE02749.1"
                     /translation="RIATAQLPREVAELGDAGPDALPKVAADAALHPVLRRRDFLELF
                     DTDPDLSGFDVDVSAWIRDADDADLLLFWRELPVAKNGFDADGQPRPAAAELCRAGIG
                     AASAFLKSRREAAFVWDGLTGQWLALRERRLHPGMTVLVAAAAGGYAPESGFAPDSRA
                     AVPPPATDDPAETVTPQDIDDDRRSFIGRYVGLAEHLADAEAAARALCTDLAEPHAEA
                     VIRAARWHDTGKAHPVFQQSLQATLPEGDPRRSPDQFWAKSEQPGGRHARPHLRHELG
                     SLLYWLTAHGWADDPASDLIGYLLLAHHGKVRTRLRALPGEQEPAPQDQYGPRFARGF
                     WEREALPALRFADGAEVPAVSVDLELMELGRGAHGPSWTARVQRLLALHGPFRLAWLE
                     TLVRIADWRASEQEQEPKREDTP"
BASE COUNT          189 a          485 c          540 g          200 t
ORIGIN      
        1 gcggtcgagc cggctttcga gcacgaagcc gtcgtcggtc cagtgtccgg tggcctcggc
       61 atcggcctgt tcggcgacga ggcgcagcac gccgagcgcc ttcaggtaat gcgcgagcgg
      121 caccggcgtg cagccgcgca gcagcagggg atgcggcgtc gggctcatgg cgtgtcctcc
      181 cgctttggtt cctgttcctg ctccgaggcc cgccagtcgg cgatgcgtac cagggtttcg
      241 agccacgcca gccggaacgg gccgtgcagt gccagcagtc gctgcacgcg cgccgtccac
      301 gacggcccgt gcgcgccgcg gccgagttcc atcagttcga gatcgacgct gacggccggt
      361 acttcggcgc cgtcggcaaa gcgcagcgcc ggcagggctt cgcgttccca gaagccgcgg
      421 gcgaagcgcg ggccgtattg atcctgcggc gccggttcct gttcgccggg cagcgcgcgc
      481 agccgggtgc ggaccttgcc gtgatgcgcg agcagcagat agccgatgag atcgctggcc
      541 gggtcgtcgg cccagccgtg cgcggtcagc cagtacagca gcgagccgag ttcgtggcgc
      601 aggtgcgggc gcgcatgccg gccgccgggc tgctccgact tggcccagaa ctgatccgga
      661 ctccggcgcg gatcgccttc gggcaaggtg gcctgcagcg attgctggaa caccgggtgc
      721 gccttgccgg tgtcgtgcca gcgcgcggcg cggatcacgg cttcggcatg cggttcggcc
      781 agatcggtac agagcgcgcg ggcagcggcc tcggcatcgg ccaggtgctc ggcgagtccg
      841 acgtaacggc cgatgaagct gcgccggtcg tcgtcgatgt cctgcggggt cacggtctcg
      901 gccgggtcgt cggtggcggg cggcggcacc gcagcgcggc tgtcgggcgc gaaaccggat
      961 tccggcgcat aaccgccggc cgcggccgcg accagcacgg tcatcccggg gtgcagcctg
     1021 cgctcgcgca gcgccagcca ctggccggtc agcccgtccc agacgaaagc ggcctcgcgg
     1081 cgcgacttca ggaaggcgct cgcggcgccg atgccggcgc ggcagagttc ggcggctgcg
     1141 gggcgcggct ggccgtcggc gtcgaaaccg ttcttcgcga ccggcaactc gcgccagaac
     1201 agcagcaggt cggcatcgtc ggcgtcgcgg atccaggccg agacatcgac atcgaagccg
     1261 ctcaggtccg gatcggtatc gaacagttcg aggaagtcgc gccggcgcag caccggatgc
     1321 agcgccgcgt cggcggcgac tttcggcagg gcatcggggc cggcatcgcc gagttcggcc
     1381 acctcgcgcg gcagctgcgc ggtggcgatg cggt
//
LOCUS       DAOQJH010000727         1398 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_93515_length_1398_cov_2.874162, whole genome shotgun sequence.
ACCESSION   DAOQJH010000727 DAOQJH010000000
VERSION     DAOQJH010000727.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1398)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1398)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1398
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93515_length_1398_cov_2.874162"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..487)
                     /locus_tag="PLQ18_13670"
     CDS             complement(<1..487)
                     /locus_tag="PLQ18_13670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013034377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HNE02750.1"
                     /translation="MAELNARAATPAPSRRERLARRLWSGLVAALVPLALARLWWRGR
                     RSPGYRERFAERFGAAPRLAPGAIWLHAVSLGETRAALPLIRALLARHPGRPLPATTT
                     TPTGSAQVREALGERVAHCYAPWDLPGACARFLDRAQPALAVIMETELWPNLLAALKR
                     RG"
     gene            complement(475..>1398)
                     /locus_tag="PLQ18_13675"
     CDS             complement(475..>1398)
                     /locus_tag="PLQ18_13675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02751.1"
                     /translation="PDLSGVSVLSEHPLRWTLPGQPGRSLLDRLLRRSPPLPDAAAET
                     GFDIPGVLRHPMRRGSLSTQEGFIVDNRSLLGALLDPVRDALVHTQGAAVMARDGGFN
                     LRIEEREPVVVRARRTVYTTGVGANLLGSVSLQVWHQQMVMIRGEGLADDLYINAVNE
                     AGRRLFNISSHRDLTGRPVWYYGGAMVESGEQVDAARQIRNCRRDLGAYFPWMALSGL
                     EFAAHRVDRAAVRMRNGLALLPQLRVEDQRVVAWPTNLSTMPLLGDRMLAMLRREGIE
                     PRPADLHLLADWPQPALAPAPWDEEAQTWQS"
BASE COUNT          203 a          514 c          519 g          162 t
ORIGIN      
        1 tcccgcgacg cttcaacgcg gccagcaggt tcggccagag ttcggtttcc atgatcacgg
       61 cgagcgccgg ctgcgcacga tcgagaaagc gcgcgcaggc cccgggcagg tcccagggcg
      121 cgtagcagtg cgcgacgcgc tcgccgagcg cctcacgcac ctgcgccgag ccggtcggcg
      181 tcgtcgtcgt cgccggcagc ggccggccgg ggtggcgcgc gagcagcgcc cggatcagcg
      241 gcagcgccgc gcgcgtctcg ccgagggaga ccgcgtgcag ccagatcgcg ccgggcgcga
      301 gccgcggtgc cgcgccgaaa cgctcggcga agcgctcgcg atagccggga ctgcgccggc
      361 cgcgccacca gaggcgcgcc agcgcgagcg gcaccagcgc cgcgaccagc ccggaccaca
      421 gccgccgggc caggcgctcg cgccggctgg gcgcgggcgt cgcggcacgg gcgttcagct
      481 ctgccatgtc tgggcctctt cgtcccacgg cgccggggcc agcgccggct gcggccagtc
      541 ggcgagcaga tgcaggtcgg ccggccgcgg ctcgatgccc tcgcgcctga gcatcgccag
      601 catgcggtcg ccgagcagcg gcatcgtcga caggttggtc ggccacgcga ccacgcgctg
      661 gtcctcgacg cgcagctgcg gcagcagcgc cagaccgttg cgcatgcgca cggcggcgcg
      721 atcgacgcgg tgcgcggcga attcgaggcc gctcagcgcc atccacggga aatacgcgcc
      781 gagatcgcgc cggcagttgc ggatctgccg ggcggcatcg acctgctcgc cggactcgac
      841 catcgcgccg ccgtagtacc agaccggccg acccgtgagg tcgcggtggg aactgatgtt
      901 gaacagccgc cggccggcct cgttgacggc attgatgtag aggtcatcgg ccagtccctc
      961 gccgcggatc atgaccatct gctgatgcca gacctgcagc gacacgctgc cgagcaggtt
     1021 cgcgccgacg ccggtggtat agaccgtgcg ccgggcacgc acgaccaccg gctcgcgctc
     1081 ctcgatgcgc aggttgaagc cgccgtcgcg ggccatgacc gccgcgccct gcgtgtgcac
     1141 cagcgcatca cgcaccggat cgagcagcgc accgagcagc gagcggttgt cgacgatgaa
     1201 gccttcctgc gtcgacaggc tgccgcggcg catcggatgg cgcagcacgc cggggatgtc
     1261 gaaaccggtc tcggcagcgg catccggcag cggcggcgaa cggcgcagca ggcgatcgag
     1321 caggctgcgc ccgggctgcc cgggcagggt ccagcgcagc ggatgctcgc tcagcacgct
     1381 gacgcccgag aggtccgg
//
LOCUS       DAOQJH010000728         1389 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_94770_length_1389_cov_3.205397, whole genome shotgun sequence.
ACCESSION   DAOQJH010000728 DAOQJH010000000
VERSION     DAOQJH010000728.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1389)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1389)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1389
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94770_length_1389_cov_3.205397"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1389
                     /locus_tag="PLQ18_13680"
     CDS             <1..>1389
                     /locus_tag="PLQ18_13680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NERD domain-containing protein/DEAD/DEAH box
                     helicase"
                     /protein_id="HNE02752.1"
                     /translation="SVTGAAQREIETLQRLADALPDDYSVYHAVHWTNLERGYSVFGE
                     IDFIVMNRAGHLLVIEQKAGFLDETPEGLVKRYPGRVKSVVAQLARNVDALRGKLNAR
                     PDAPAAQIDYLFYCPDYHVRKPHSAGLAPERIVDASRRDELPALIQGVLPAGETGGGR
                     SDEAQKVHRFLCDVIQLETDVSALIGRARALVGRISGGLAHWARQLEFAPFHLRVTGT
                     AGSGKTQLALAEFRATLERGQRPLYLCYNRPLADHFRAIAPEGGLIATFHAFCQQRLR
                     AAGQPPDFSQPDAFDRLIADAARLPEDEAYRFDTVIVDEGQDFSEAWRDLAFRHARPE
                     ARRIWLEDPMQNLYAREPVPLPGWVGIRSFANFRCPRSVVRMLQDILPVDMPIEAASP
                     IDASEVEILTYSDNASLLQALKEGIRLCYSESFRKEDVAVVTYSGRNNSRLLTYDRIG
                     PHTMRRFTGEYD"
BASE COUNT          217 a          503 c          443 g          226 t
ORIGIN      
        1 tctcggtcac cggtgccgcg cagcgcgaga tcgaaaccct gcagcggctg gccgacgccc
       61 tgcccgacga ttacagcgtc tatcacgcgg tgcactggac caacctcgag cgcggctatt
      121 cggtattcgg cgagatcgac ttcatcgtca tgaaccgtgc cgggcacctg ctcgtcatcg
      181 aacagaaggc cggcttcctc gacgagacgc cggaaggcct cgtcaagcgc tatcccggcc
      241 gcgtcaagag tgtcgtcgcg cagctggcac gcaacgtcga cgcgctgcgc ggaaagctca
      301 acgcgcgtcc cgatgcgccg gcggcgcaga tcgactacct cttctactgc cccgactacc
      361 atgtccgcaa gccgcacagt gcgggccttg cgccggagcg catcgtcgac gcgtcgcgcc
      421 gcgacgagtt gccggcgctg atccagggcg tgctgccggc gggcgagacg ggcggcggtc
      481 gcagtgatga ggcgcagaag gtgcatcgct tcctgtgcga cgtgatccag ctcgaaaccg
      541 atgtcagcgc gctgatcggc cgggcgcggg cgctggtcgg ccgcatttcc ggcgggctcg
      601 cgcactgggc acggcaactg gaattcgccc cgtttcatct gcgcgtcacc ggtactgccg
      661 gctccggcaa gacgcaactg gcgctggccg agttccgcgc gacgctcgag cgcggccagc
      721 ggccgctcta cctctgctac aaccggccgc tggccgacca cttccgcgcc atcgcccccg
      781 agggcggctt gatcgccacc ttccatgcct tctgccagca gcggctgcgc gccgccggtc
      841 agccccccga ctttagccag cccgatgcct tcgaccgcct gatcgcggat gcggcgcgct
      901 tgccggagga cgaggcttac cgcttcgata cggtgatcgt cgatgaaggt caggatttct
      961 ccgaggcctg gcgtgacctc gccttccgcc atgcgcggcc ggaggcgcgg cggatctggc
     1021 tggaggaccc gatgcagaat ctctatgcgc gcgagccggt gcccttgccc ggctgggtcg
     1081 gcatccgctc cttcgccaac ttccgctgcc cgcgctcggt cgtgcgcatg ctgcaggaca
     1141 tcctgccggt cgacatgccg atcgaggccg cttcgccgat cgacgccagc gaagtcgaga
     1201 tcctcaccta cagcgacaat gcctcgctgc tgcaggcgct caaggaaggc atccggctct
     1261 gctacagcga aagcttccgc aaggaggatg tcgccgtcgt cacctacagc ggccgcaaca
     1321 actcgcgcct gctgacctac gaccgcatcg gtccgcacac gatgcgccgc ttcaccggcg
     1381 agtacgacc
//
LOCUS       DAOQJH010000729         1388 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_94939_length_1388_cov_3.003001, whole genome shotgun sequence.
ACCESSION   DAOQJH010000729 DAOQJH010000000
VERSION     DAOQJH010000729.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1388
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94939_length_1388_cov_3.003001"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="PLQ18_13685"
     CDS             <1..200
                     /locus_tag="PLQ18_13685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HNE02753.1"
                     /translation="NPNSIRVIKAWLEPALKHARAEERFQFERHGYFVADRVDSQPGA
                     PVFNRTLTLKDSWSAKPGRPR"
     gene            complement(223..606)
                     /gene="arsC"
                     /locus_tag="PLQ18_13690"
     CDS             complement(223..606)
                     /gene="arsC"
                     /locus_tag="PLQ18_13690"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006746035.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HNE02754.1"
                     /translation="MDEPPVRIYHHPRCSKSRAACQLIADNGIVAEVIDYLKTPPDRE
                     ELCRLLRLLGMRPAELVRRGELVFKEHYAARTLDDEQWLDALLAHPILIERPIIVRGD
                     RAVLGRPPEKVLELLQGSISAGVDR"
     gene            complement(660..1271)
                     /locus_tag="PLQ18_13695"
     CDS             complement(660..1271)
                     /locus_tag="PLQ18_13695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNE02755.1"
                     /translation="MFSVYGIGGPIFRGTFEGLKDVAGIFRSTAVRPVAGEDQRAALS
                     APAAQAVSAYREMLRTDTDRGPLYHAYQIMQRQVVSVSSEDAVEHAWQVLLEQRIHQA
                     PVLDPGYRLVGVVSERDLLTVLNVDAGRVRDVLARKVADVMSTPVVCAHPITDIRRIA
                     HVLLEHQVDGVPIINETEALVGFVSRSDIVRAIVADPPLSLWR"
BASE COUNT          239 a          452 c          451 g          246 t
ORIGIN      
        1 tcaacccgaa cagcattcgg gtcatcaagg cctggctcga gcccgcgctg aagcatgcca
       61 gggccgagga gcgttttcag ttcgaacggc acggctactt cgttgccgac cgcgtcgatt
      121 cgcagcccgg tgcgccggtg ttcaaccgga cgttgacgct caaggattcg tggtcggcca
      181 agcccggccg accgcgctga gtctaccgac ctacggctgt cgctaccggt cgactccggc
      241 ggagatgctt ccctgcagca actccagtac cttctccggc ggccgtccga gaacggcccg
      301 gtcgccgcgc acgatgatcg ggcgctcgat caggatcgga tgcgcaagca gtgcatccag
      361 ccattgctcg tcgtccaggg tacgcgccgc gtagtgttcc ttgaacacca gttcgccgcg
      421 gcgcacgagt tcggctggcc gcatgccgag caggcgcagc aagcggcaca gttcttcccg
      481 gtccggaggc gtcttcaggt agtcgatgac ctcggccacg atgccgttgt cggcgatcaa
      541 ctggcaggcc gcgcggctct tcgaacaacg cgggtggtga tagatgcgta caggcggttc
      601 gtccatcgtt gatcctcgtc gaaatcacgc tcggaaatac agtggggccg gcgacggctc
      661 tagcgccaca agctcagcgg cggatcggcc acgatggcgc gcacgatgtc gctgcgcgaa
      721 acgaagccga ccagggcctc ggtttcgttg atgatcggta cgccatcgac ctggtgttcg
      781 agcaggacat gtgcgatgcg ccggatgtcg gtaatcgggt gcgcacagac caccggcgtg
      841 ctcatgacat cagcgacctt gcgggccagg acatcgcgga cgcggcccgc gtcgacgttg
      901 agcacggtca gcaagtcccg ttcgctgacg acgccaacca ggcggtagcc ggggtcgagg
      961 accggcgcct ggtgaatacg ctgttcgagc aggacttgcc acgcgtgttc aacggcgtct
     1021 tcgctcgaca ccgaaacgac ctgccgctgc atgatctggt aggcatggta gagggggccg
     1081 cgatcagtgt ccgttcgcag catctcccgg taggcgctca ccgcctgcgc ggcgggcgca
     1141 ctcagcgcgg cgcgctggtc ttctccggcg accggacgca cagccgtgct gcggaagatt
     1201 ccggcgacat ccttcaggcc ttcgaatgtg ccacggaaaa tcggtccgcc gataccataa
     1261 accgaaaaca cgctgttctc ctcgtcgggt actgcacctc gtcatccgcc acaagctgcg
     1321 gcccgccgga gcgggccgca gcttgtggcg attgtagccg tctttagcgc gacttgcgtg
     1381 acgcggct
//
LOCUS       DAOQJH010000730         1368 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_97868_length_1368_cov_1.715156, whole genome shotgun sequence.
ACCESSION   DAOQJH010000730 DAOQJH010000000
VERSION     DAOQJH010000730.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1368)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1368)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1368
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97868_length_1368_cov_1.715156"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1368)
                     /locus_tag="PLQ18_13700"
     CDS             complement(<1..>1368)
                     /locus_tag="PLQ18_13700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNE02756.1"
                     /translation="FSNITGYAEGEVLGRNMRMLQSGYEGRAFYLDFWRALQQSGHWQ
                     GEIRNRRRSGEPYLQWLTINAVRDADQTIIRYVGVFSDITAVRNAHDLEHLAHHDALT
                     GLPNRLLLIAELDQALRRAARSGGGLAVLFLDLDRFKLVNDSFGHTAGDELLVRAAGR
                     LRERLRQSDLLARLGGDEFVVVLEEVTEPERAAGVAQTLIEALQQPFLLGGGHEVWIG
                     ASVGISLYPADGAEAGALIQRADTALYRAKEDGRGNARFYLEAMTRAVEERLVLEARL
                     RRALERGELVLYYQPQIMLGEPAALGVEALVRWRRPDGKMVPPSDFIPLAEETGLIVP
                     LGEWVLREACRCLRRWLADGVPIHSMAVNLSARQLRLHALAPQLAAILDETGVPGRYL
                     ELELTESALMENPDTVAQQLEALRRLGVSLAIDDFGTGYSSLAYLKRFPIDKLKIDRS
                     FVLDIP"
BASE COUNT          212 a          473 c          477 g          206 t
ORIGIN      
        1 gggatgtcga gcacgaagct gcggtcgatc ttcagcttgt cgatcgggaa gcgcttcagg
       61 taggcgagcg aggaatagcc ggtgccgaag tcgtcgatcg cgaggctgac gccgagccgg
      121 cgcagggctt cgagctgctg ggcgaccgtg tccgggtttt ccatcagcgc gctctcggtc
      181 agttcgagtt cgagataccg ccccggcacg ccggtctcgt cgaggatggc cgcgagctgc
      241 ggcgccagcg cgtgcaggcg cagctgccgg gccgacaggt tcacggccat cgagtggatc
      301 ggcacgccat cggccagcca gcggcgcagg cagcggcagg cctcgcgcag cacccattcg
      361 ccgagcggca cgatcagccc ggtttcctcg gcgagcggga tgaagtcgga cggcggcacc
      421 atcttgccgt ccggccgccg ccagcgcacc agcgcctcga ccccgagggc cgccggttcg
      481 ccgagcatga tctgcggctg gtagtacagc accagctccc cgcgttcgag cgcacggcgc
      541 aggcgcgcct ccagcaccag gcgctcctcg acggcgcgcg tcatcgcctc cagatagaag
      601 cgcgcattgc cgcgaccgtc ctccttggcg cgatacagcg cagtgtcggc acgctggatc
      661 agcgcgccgg cctcggcccc gtcggccggg tacaggctga tgccgacgct ggcaccgatc
      721 cagacctcgt ggccgccgcc gagcaggaag ggctgctgca gggcttcgat cagcgtttgc
      781 gcgacgccgg cggcacgctc gggttcggtg acctcctcaa gcacgaccac gaactcgtcg
      841 ccgccgaggc gcgcgagcag gtcggactga cgcagccgtt cgcgcagccg gccggccgcc
      901 cgcaccagca gctcgtctcc ggcggtgtga ccgaagctgt cattgaccag cttgaagcgg
      961 tcgagatcga gaaacagcac cgccaggccg cctccgctgc gcgcggcgcg gcgcagcgcc
     1021 tggtcgagtt cggcgatcag cagcagccgg ttcggcagtc cggtcagcgc gtcgtggtgc
     1081 gcgaggtgtt cgagatcgtg ggcgttgcgg accgccgtga tgtcggagaa cacgccgaca
     1141 tagcggatga tcgtctggtc ggcatcgcgc acggcgttga tcgtcagcca ctgcagatag
     1201 ggttcaccgc tgcgccggcg attgcggatc tcgccctgcc agtgcccgct ctgctgcagc
     1261 gcgcgccaga aatccagata gaacgcccgg ccctcgtagc cggactgcag catgcgcatg
     1321 ttgcggccga ggacttcgcc ttccgcataa cccgtgatgt tcgagaat
//
LOCUS       DAOQJH010000731         1367 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_97932_length_1367_cov_2.961890, whole genome shotgun sequence.
ACCESSION   DAOQJH010000731 DAOQJH010000000
VERSION     DAOQJH010000731.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1367
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97932_length_1367_cov_2.961890"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..481)
                     /locus_tag="PLQ18_13705"
     CDS             complement(<1..481)
                     /locus_tag="PLQ18_13705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNE02757.1"
                     /translation="MTTPIPAAADLEALWMPFTANRQFKADPRLLVAAEGMHYTSHDG
                     RRVLDAVAGLWCVNAGHGRRRIVEAVQRQVAEMDYAPPFQMGHPAAFELAGRLTALTQ
                     GKMGQVFFTNSGSESVDTALKIAIAYHRLRGEGTRTRLIGRERGYHGVGFGGISVGGI
                     "
     gene            817..>1367
                     /locus_tag="PLQ18_13710"
     CDS             817..>1367
                     /locus_tag="PLQ18_13710"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNE02758.1"
                     /translation="MSVSPPPPIRVLLVDDHAVVRAGFRRLLEGCGVRVSAEADTGEA
                     GYRAYVDAPCEVVILDLALPGRGGLETLRRLRARDAAARVLVFTMHDEPAFAEQALAA
                     GALGYVTKSSSPEVLLDAVTRVARGERCLSPDIAERLALAPSGGPQAAIEALSPREFE
                     IFRQLAAGHSAAEIGERLALSAKT"
BASE COUNT          183 a          505 c          483 g          196 t
ORIGIN      
        1 cgatgccgcc gaccgagatg ccgccgaagc cgacgccgtg gtagccgcgc tcgcgcccga
       61 tcagccgcgt gcgcgtgccc tcgccgcgca ggcggtggta ggcgatcgcg atcttcagcg
      121 ccgtgtcgac cgactcggag ccggagttcg tgaagaacac ctggcccatc ttcccttgcg
      181 tcagcgccgt cagccggccg gccagctcga aggccgccgg gtggcccatc tggaacggcg
      241 gcgcgtagtc catctcggcg acttggcgct gcacggcctc gacgatgcgc cggcggccgt
      301 ggccggcgtt gacgcaccac agtccggcga ccgcgtcgag cacgcgccgg ccgtcgtggc
      361 tggtgtaatg catgccttcg gcggcgacga gcaggcgcgg atcggccttg aactggcggt
      421 tggccgtgaa cggcatccac agcgcttcga gatcggccgc agccgggatc ggggtggtca
      481 tcggcagggg ctcccgcggt cagaggcaga tcgccagaac ctatgcccgt cgaggaaaat
      541 gcacaagccc ggggcaggct taccaaacgt acagctgcca tgccgggtgc gctgcgatca
      601 gcccgcgcct gatcgttcaa aattttcaac acatgagccg ggcaccggcg cggcgcgact
      661 gtgccggccg atgcacgcgc tgtaccgcat ccgggacagg ccggcgtccg gacgccgccg
      721 gaagcagccg gcccggcccg tccgcgcgtg tgtgcggctg gcgccgatct tgctgaaagc
      781 ccggtttccc ccgccgcccg tttaccggtc cgaaccatgt ccgtcagccc gcccccgccg
      841 atccgcgtcc tgctcgtcga tgaccacgcc gtcgtgcgcg ccggcttccg gcgcctgctc
      901 gaaggctgcg gcgtgcgcgt cagcgccgag gccgacaccg gcgaggccgg ctatcgcgcc
      961 tacgtcgatg cgccctgcga ggtcgtcatc ctcgatctgg cgctgcccgg gcgcggcggc
     1021 ctcgaaaccc tgcgccggct gcgcgcacgc gatgccgcgg cccgggtgct ggtgttcacg
     1081 atgcacgacg aaccggcctt cgccgaacag gcgctcgccg ccggcgcgct cggctacgtg
     1141 accaagtcat cgagccccga ggtgttgctc gatgccgtga cccgcgtcgc gcgcggcgaa
     1201 cgctgcctgt cgccggacat cgccgagcgg ctggcgctgg cgccgtccgg cggcccgcag
     1261 gcggcgatcg aggcgctgtc gccgcgcgag ttcgaaatct tccgccagct cgccgccggg
     1321 cattcggccg ccgagatcgg cgagcggctg gcgctgtcgg ccaagac
//
LOCUS       DAOQJH010000732         1363 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_98631_length_1363_cov_1.963303, whole genome shotgun sequence.
ACCESSION   DAOQJH010000732 DAOQJH010000000
VERSION     DAOQJH010000732.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1363)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1363)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1363
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98631_length_1363_cov_1.963303"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..125
                     /locus_tag="PLQ18_13715"
     CDS             <1..125
                     /locus_tag="PLQ18_13715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HNE02759.1"
                     /translation="AITQLAVGEALVSVLEGGYELSALASSCAAHVRALMAAGG"
     gene            453..>1363
                     /locus_tag="PLQ18_13720"
     CDS             453..>1363
                     /locus_tag="PLQ18_13720"
                     /inference="COORDINATES: protein motif:HMM:NF019874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HNE02760.1"
                     /translation="MPFLKNLSLRSQFALLLAINALAMVLALTVTLRNLHAELEHERR
                     DQVSNLVDLAWATVARYQAAEAAGQLDRATAQARAIEALLGMRSGEIYFWVHRSDGVY
                     VMHGGKPERAGTAGLLELRDAQGRRLFDSFEDALARGNGAGFVEYSFPRAGTDVPEPK
                     VSRVKRFVPWDWVIGSGLYISDVEDAYASQRNQLLVGLGAALLLLAVMLWGQSRRIVR
                     QVHAVLGLARRLAARDLSSPLPVEARDEFGQMAEALNAAVTAMQTAFTEVGEAAARDR
                     QQMEQLRAAEAAQEAQSRELATQSERIR"
BASE COUNT          190 a          488 c          478 g          207 t
ORIGIN      
        1 aagcgatcac gcaactcgcg gtgggcgaag cgctggtgtc ggtgctcgaa ggcggctacg
       61 agctttcggc gctggcatcg agctgtgcag cgcacgtgcg cgcgctgatg gccgccggcg
      121 gctgaagccg ccgggcacac ggcctcttcc cccccgacgg ccgcactcgc gcggccgtca
      181 cggttttttt cagccgctcc cgggcgccgc agttcggcat ccgcaccggc gatttcgacc
      241 ggcgatccgg gttttgccgc attgcgcaat cgcagcagcc tgccgcgacc cggcaaaccc
      301 ttaccgcccg cccggtaaat ctttgtattg gcctatagcc gcccgcccgc cctgccgata
      361 ttcagtcgcg atacccgata cgtccgcccg tggcgccggc cgtccccccc gaccgccggc
      421 cctgccgtat ccccgcaacc gggagcgtcg cgatgccttt cctgaagaac ctgtccctgc
      481 gcagccagtt cgcgctgctg ctcgcgatca acgcgctggc catggtgctg gcgctgaccg
      541 tgacgctgcg caacctgcac gccgagctgg agcacgaacg ccgtgaccag gtttcgaatc
      601 tggtcgatct ggcctgggcg acggtcgcgc gctatcaggc cgccgaggcc gccggacagc
      661 tcgaccgggc cacggcgcag gcgcgtgcca tcgaggcgct gctcggcatg cgcagcggcg
      721 agatctactt ctgggtgcat cgctcggacg gcgtctatgt gatgcacggc ggcaagccgg
      781 agcgtgccgg caccgccggg ctgctggagc tgcgggatgc gcagggcagg cgcctgttcg
      841 acagcttcga ggacgcgctc gcgcgcggca acggcgccgg cttcgtcgag tacagctttc
      901 cgcgggccgg aaccgatgtg cccgagccga aggtctcgcg ggtcaagcgc ttcgtgccct
      961 gggactgggt gatcggcagc ggcctgtaca tctccgatgt cgaggacgcc tacgccagcc
     1021 agcgcaacca gttgctcgtc ggcctcggcg cggcgctgct gctgctcgcc gtgatgctgt
     1081 gggggcagag ccggcgcatc gtgcgccagg tgcatgcggt gctcggcctc gcgcgccggc
     1141 tggcggcgcg cgatctcagc agcccgctgc cggtcgaggc gcgcgacgag ttcggacaga
     1201 tggccgaggc gctcaacgcc gcagtcaccg cgatgcagac ggccttcacg gaggtcgggg
     1261 aagccgcggc gcgcgaccgc cagcagatgg agcagctgcg cgccgccgag gccgcgcagg
     1321 aagcgcagtc acgcgagctt gcgacgcagt ccgaacgcat ccg
//
LOCUS       DAOQJH010000733         1360 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_98993_length_1360_cov_3.131801, whole genome shotgun sequence.
ACCESSION   DAOQJH010000733 DAOQJH010000000
VERSION     DAOQJH010000733.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1360)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1360)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1360
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98993_length_1360_cov_3.131801"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..139)
                     /locus_tag="PLQ18_13725"
     CDS             complement(<1..139)
                     /locus_tag="PLQ18_13725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017937665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HNE02761.1"
                     /translation="MSTTLTPVGTLHSPYREKFAIPRQPWLARLPAVLELLAPFDRPE
                     AV"
     gene            complement(136..>1360)
                     /locus_tag="PLQ18_13730"
     CDS             complement(136..>1360)
                     /locus_tag="PLQ18_13730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02762.1"
                     /translation="GLLGLALCGTQARERHALLLIDACLIYVAWKRGFVRADALHLIG
                     FFAILPVLGVLPMLGAMLWRASLQRWGEACCGLLALIALAGMFAIDRYHPVRQIDDMR
                     AGMRALLAGPQPGPVPAGDPKTRGTPGPADRLLLTQRLAGRDSVDLISYEQGRLFFND
                     LNYRPRPVIQSYAAYTPALAARNAAHFLGPQRPDFVLFNPAETIDGRLPMMDDGAALL
                     VVLGNYRALGRDGKYLLLQRRDDPPRPPQLESLAERELHWGEDVDLTAFRGEVLAFAL
                     EVTPSAAGRLAGLLFQSLPPQIVLDAGDGERRYRLVPGMLATPVLLSPHLRTSEDLLA
                     LGAPPPAAGFGHAVRRLRFEVAPPAPLLAGHEDFGRALYHDTLKLRLYRVRGLPDAGF
                     TAAYSPDPDRVRAGP"
BASE COUNT          210 a          482 c          501 g          167 t
ORIGIN      
        1 gcacggcctc cggccggtcg aacggcgcca gcagttcgag cacggccggc agccgcgcca
       61 gccacggctg gcgcgggatc gcgaacttct cgcgatacgg cgagtgcagc gtgccgaccg
      121 gggtcagcgt cgtgctcacg gtccggcgcg gacgcggtcg gggtcggggc tgtaggcggc
      181 cgtgaacccg gcgtccggca gcccgcgcac gcggtacagg cgcagcttca gcgtgtcgtg
      241 gtacagcgcc cggccgaagt cctcgtggcc ggcgagcagc ggcgccggcg gcgcgacctc
      301 gaagcgcagc cggcgcacgg cgtgaccgaa gcccgcggcc ggtggtggtg caccgagcgc
      361 aagcagatcc tcgctcgtgc gcaggtgcgg actgagcagc accggcgtgg cgagcatgcc
      421 gggcaccagc cggtagcggc gctcgccgtc gccggcatcg agcacgatct gtggcggcag
      481 gctctggaac agcagcccgg ccagccgccc ggccgcgctt ggcgtgacct ccagcgcgaa
      541 ggccagcacc tcgccacgga aggccgtcag atcgacatcc tcgccccagt gcagctcgcg
      601 ctcggccagc gactccagct gcggcggccg gggcggatcg tcgcgccgct gcagcagcag
      661 gtacttgccg tcgcgaccga gcgcccggta gttgccgagc accacgagca gggccgcgcc
      721 gtcatccatc atcggcagcc ggccgtcgat ggtctcggcc gggttgaaca gcacgaaatc
      781 cgggcgctgc gggccgagga aatgcgcggc gttgcgcgcg gcgagcgctg gcgtgtaggc
      841 cgcgtaactc tggatgaccg ggcgcgggcg atagttgaga tcgttgaaga acagcctccc
      901 ctgttcataa ctgatcaggt cgacgctgtc cctgccggcg aggcgctgcg tcagcagcag
      961 ccggtcggcc ggacccggtg tgccgcgtgt cttcggatcg ccggccggca ccggcccggg
     1021 ctgcggtccg gcgagcagcg cccgcatgcc cgcgcgcatg tcgtcgatct ggcggaccgg
     1081 gtgatagcga tcgatggcga acatgccggc cagcgcgatc agcgcgagca atccgcagca
     1141 tgcctcgccc cagcgctgca gcgacgcccg ccatagcatg gcgccgagca tcggcaggac
     1201 gccgagcacg ggcaggattg cgaagaaacc gatcaggtgc agggcgtcgg cgcgcacgaa
     1261 gccgcgcttc caggccacgt agatcaggca ggcatcgatc agcagcagcg catggcgttc
     1321 ccgtgcctgc gttccgcaca gcgccaaccc cagaagcccc
//
LOCUS       DAOQJH010000734         1352 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_100155_length_1352_cov_4.569776, whole genome shotgun
            sequence.
ACCESSION   DAOQJH010000734 DAOQJH010000000
VERSION     DAOQJH010000734.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1352)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1352)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1352
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100155_length_1352_cov_4.569776"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..826)
                     /gene="nadA"
                     /locus_tag="PLQ18_13735"
     CDS             complement(<1..826)
                     /gene="nadA"
                     /locus_tag="PLQ18_13735"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018076914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase"
                     /protein_id="HNE02763.1"
                     /translation="MNAAVPVRSHRLTPEQVPPTIVETLEAGERAELTARIRSLLKAQ
                     DATLVAHYYTSPELQELADATGGYVSDSLDMARFGTECPSKTLIVAGVRFMGETAKIL
                     NPEKRVLMPDLHAECSLDLGCPPDEFSAFCDAHPDREVVVYANTSAAVKARADWMATS
                     GIALDLVRHLHAQGKKILWAPDKHLGAWVRDQTGADVLLWDASCVVHEAFKADGLVQM
                     KLEHPDALVLVHPESPAEVIALADYVGSTTGIIKAAKELPAQKFIIATDNGIFHKMR"
     gene            complement(862..>1352)
                     /locus_tag="PLQ18_13740"
     CDS             complement(862..>1352)
                     /locus_tag="PLQ18_13740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005958933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNE02764.1"
                     /translation="ALRHLAARTGVRGVWLEQLYTFGRPDRDPAQRVIAVAYYALVPI
                     GQLEVGPADQHRDWFPVRALPRLALDHAAIVTRALDRLVAKLDYSTIALQFMPARFTL
                     SALQAVYETILGEALDKRNFRKRVLALACIEDTGELSREGHHRPARLYRVARPGTVEF
                     IK"
BASE COUNT          205 a          451 c          486 g          210 t
ORIGIN      
        1 cgcgcatctt gtggaagatg ccgttgtcgg tcgcgatgat gaacttctgc gccggcagct
       61 ccttcgcggc cttgatgatg ccggtggtcg agccgacgta atcggcgagc gcgatgacct
      121 cggccggcga ttccggatgc acgagcacca gcgcgtccgg atgttcgagc ttcatctgca
      181 ccagaccgtc ggccttgaag gcttcgtgca cgacgcagga ggcatcccag agcagcacgt
      241 cggcaccggt ctggtcacgc acccaggcac cgaggtgctt gtccggcgcc cacaggatct
      301 ttttgccctg cgcgtgcagg tggcggacca gatcgagcgc gatgcccgag gtcgccatcc
      361 agtcggcgcg cgccttcacg gccgccgagg tgttggcgta gacgacgacc tcacggtccg
      421 gatgcgcgtc gcagaaggcc gagaactcgt cgggcgggca gccgagatcg agcgagcact
      481 cggcgtgcag gtccggcatc agcacgcgct tctcgggatt caggatcttc gcggtctcgc
      541 ccatgaagcg cacgccggcg acgatcagcg ttttcgacgg gcactcggtg ccgaagcgcg
      601 ccatgtcgag cgaatccgac acgtagccgc cggtggcatc ggcgagttcc tgcagctccg
      661 gcgaggtgta gtagtgcgcg accagcgtcg cgtcctgcgc cttcagcagg ctcctgatgc
      721 gcgcggtcag ctcggcgcgc tcgccggcct ccagcgtctc gacgatggtc ggcggcacct
      781 gctcgggcgt cagccggtgg ctgcggacag gaacggctgc gttcatgggg tgggctccgg
      841 gcggccgcgg ggcgggccgc atcacttgat gaattcgacg gtgccgggtc gcgcgacccg
      901 atacagccgt gccggccggt ggtgtccctc gcgggacagc tcgccggtgt cttcgatgca
      961 cgcgagcgcg agcacgcgct tgcggaagtt ccgcttgtcc agcgcctcgc cgaggatggt
     1021 ctcgtacacc gcctgcagcg ccgacagcgt gaagcgcgcc ggcatgaact gcagggcgat
     1081 ggtcgaatag tcgagcttgg cgaccagccg atccagcgcg cgcgtgacga tcgccgcgtg
     1141 atcgagcgcg agccgcggca gcgcgcgcac cgggaaccag tcgcggtgct gatcggccgg
     1201 accgacctcg agctggccga tcggcaccag cgcgtagtac gcgaccgcga tcacgcgctg
     1261 cgccgggtcg cgatccggcc ggccgaaggt atacagctgt tcgagccaga cgccgcgcac
     1321 gccggtgcgg gccgcgagat gacgaagcgc cg
//
LOCUS       DAOQJH010000735         1351 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_100292_length_1351_cov_5.203704, whole genome shotgun
            sequence.
ACCESSION   DAOQJH010000735 DAOQJH010000000
VERSION     DAOQJH010000735.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1351)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1351)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1351
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100292_length_1351_cov_5.203704"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            308..>1351
                     /gene="asnB"
                     /locus_tag="PLQ18_13745"
     CDS             308..>1351
                     /gene="asnB"
                     /locus_tag="PLQ18_13745"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019916738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HNE02765.1"
                     /translation="MCGIAGIVDLRERRNIEADVLASMNQVQFHRGPDEGGVHIEPGV
                     GLAHRRLSIIDLSSGQQPLWNEDHTVVVVFNGEIYNYQGIAAELQALGHTFRTHSDTE
                     VIVHAWEQWGEACVTRFRGMFAFALWDRNTETLFLARDRLGKKPLHYALLPDGQLIFG
                     SELKILAAHPRFPRALDPLAVEDYFAYGYIPDPRSIYRAALKLPPAHTLCLRRGEPLP
                     APKSYWDAPFAPLPAMSVEDAGAELIERLREATRIRLMSEVPLGAFLSGGVDSSAVVA
                     MMAGLSDEPVKTCSIAFDDPRFNEAQFAQQVADRFKTEHRVETVDPDDFDLIDRLAWL
                     YDEPYADSSALPTY"
BASE COUNT          225 a          467 c          451 g          208 t
ORIGIN      
        1 agatccagag ggcatccatg tgcggcgggc ggcgatcaag gacgggggct gaggcagggc
       61 gcgcacgcta gcacagtggt gacgggccgc gcaggccggg gcatcgtgga cctgagcggc
      121 atcacttgac gcaagttgac cgaatccgac tgcggccggc gggatggccc ggcataatgc
      181 ggcaccgcaa cgacagggaa tcccgatggc cggcctgctt ttccggctgc ggatcgctgc
      241 gtgcaggccg tcggcgaggg atcacgcctt cgaatgaccc aacacgaacc cggagtggca
      301 gcgcaccatg tgcggaatcg caggcatcgt cgacttgcgc gaacggcgga acatcgaggc
      361 agacgtgctc gcgtcgatga accaggtcca gttccaccgc ggccccgacg agggcggcgt
      421 gcacatcgag cccggggtcg gcctcgcgca ccggcggctg tcgatcatcg acctgtcgag
      481 cggccagcag ccgctctgga acgaggatca cacggtcgtc gtcgtcttca acggcgagat
      541 ctacaactac cagggcatcg ccgccgaact gcaggcgctc gggcatacgt tccgcacgca
      601 tagcgatacg gaagtcatcg ttcatgcctg ggagcagtgg ggcgaggcct gcgtcacgcg
      661 ctttcgcggc atgttcgcgt tcgcgctgtg ggaccgcaac accgaaaccc tgttcctggc
      721 ccgcgaccgg ctcggcaaga agccgctgca ctacgcgctg ctgccggacg gccagctgat
      781 cttcggctcg gagctgaaga tcctcgccgc gcatccgcgc ttcccgcgcg cgctcgatcc
      841 gctcgcggtc gaggactact tcgcctacgg ctacatcccc gatccgcgct cgatctaccg
      901 cgcggcgctg aagctgccac cggcgcacac gctgtgcctg cgccgcggcg agccgctgcc
      961 ggcgccgaag tcctactggg acgcaccgtt cgcgccgctg ccggcgatga gcgtcgagga
     1021 tgccggtgcc gagctgatcg aacgcctgcg cgaggcgacg cgcatccggc tgatgtcgga
     1081 agtgccgctc ggcgcgtttc tgtccggcgg cgtcgattcg agcgccgtcg tcgcgatgat
     1141 ggccggcctg tccgacgaac cggtcaagac ctgctcgatt gccttcgacg acccgcgctt
     1201 caacgaggcg cagttcgcgc agcaggtcgc cgaccgattc aaaaccgaac accgcgtcga
     1261 gaccgtcgat cccgacgact tcgacctgat cgaccggctc gcctggctgt atgacgagcc
     1321 ctacgccgat tcctcggcgc tgccgaccta c
//
LOCUS       DAOQJH010000736         1346 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_101103_length_1346_cov_3.574748, whole genome shotgun
            sequence.
ACCESSION   DAOQJH010000736 DAOQJH010000000
VERSION     DAOQJH010000736.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1346
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101103_length_1346_cov_3.574748"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..807)
                     /locus_tag="PLQ18_13750"
     CDS             complement(<1..807)
                     /locus_tag="PLQ18_13750"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNE02766.1"
                     /translation="MNRYLAPAALLPEGWARRVLLEVDAHGCLGRVEPDCPPAQAEGA
                     EVLAGPVLPGLANLHSHAFQRALAGLAQRAERRDESFWSWRERMYALVAHLSPEDIQT
                     LARRVYIELLKAGWTAVAEFHYLHRDARGRAYADPAELALRIVAAARETGIGLTLLPA
                     LYRLGGFGERPVAGAQKRFAIGVDDFLHLLERVREHGAREGFVTGTALHSLRAVAAAD
                     MRTVLQTLPRASVIHIHIAEQRREVDECLAWSGRRPLDWLFAHAPVDARWC"
     gene            941..>1346
                     /locus_tag="PLQ18_13755"
     CDS             941..>1346
                     /locus_tag="PLQ18_13755"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011070507.1"
                     /note="catalyzes the formation of
                     4-imidazolone-5-propanoate from urocanate during histidine
                     metabolism; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HNE02767.1"
                     /translation="MQTRHDPDRLIRAPRGPGLRCRNWQAEAALRMLMNNLDPEVAER
                     PSELVVYGGIGRAARDWSCYDRIVATLERLADDETLLIQSGKPVGVFRTHAEAPRVLL
                     ANSNLVPHWANWEHFNALDRAGLMMYGQMTAGS"
BASE COUNT          193 a          495 c          489 g          169 t
ORIGIN      
        1 gcaccagcgc gcatcgaccg gcgcgtgcgc gaacagccag tcgagcggcc ggcgcccgga
       61 ccaggccagg cattcgtcga cctcgcgccg ctgctcggcg atgtggatgt ggatgacgct
      121 ggcgcgcggc agcgtctgga gcaccgtgcg catgtcggcc gccgcgaccg cgcgcagcga
      181 atgcagcgcg gtgccggtca cgaagccctc gcgcgcgccg tgctcgcgca cgcgttcgag
      241 caggtgcagg aagtcatcga cgccgatcgc gaagcgcttc tgcgcgccgg caaccgggcg
      301 ctcgccgaag ccgccgagcc ggtacagcgc cggcagcagc gtcaggccga tgccggtctc
      361 gcgcgccgcc gcgacgatgc gcagcgccag ctccgccgga tcggcgtagg cccggccccg
      421 ggcgtcgcgg tgcaggtagt ggaactcggc gaccgcggtc cagccggcct tcagcagctc
      481 gatgtagacg cgccgcgcca gcgtctggat gtcctcgggg ctcaggtgcg cgaccagcgc
      541 gtacatgcgc tcgcgccagc tccagaagct ctcgtcgcgg cgctcggcac gctgggccag
      601 cccggccagc gcgcgctgga aggcgtgcga gtgcaggttg gcgagccccg gcagcaccgg
      661 gccggcgagg acttcggcgc cttcggcctg cgccggcgga caatccggct cgacccggcc
      721 gaggcagccg tgcgcgtcga cttcgagcag cacgcgccgg gcccagccct cgggcagcag
      781 ggcggcggga gcgagatagc ggttcatggc gattctcctt gcggctggca gccggacccg
      841 gtccggaatg cacgggcggg ggtgcggggg cgcgccgcgg gtgccaagct gcctgtacct
      901 gcatggcata gcccatcctg gcactgccgg aggtcgccga atgcaaaccc gtcacgatcc
      961 ggaccgactg atccgcgccc cgcgcggccc cgggctgcgc tgccgcaact ggcaggccga
     1021 agccgcgctg cgcatgctga tgaacaacct cgaccccgag gtcgccgagc gcccgtccga
     1081 gctggtcgtc tacggcggca tcggccgcgc cgcgcgcgac tggagctgtt acgaccgcat
     1141 cgtcgcgacg ctcgaacgcc tcgccgacga cgaaaccctg ctgatccagt ccggcaagcc
     1201 ggtcggcgtg ttccgcaccc acgccgaagc accgcgcgtg ctgctcgcca acagcaatct
     1261 ggtgccgcac tgggcgaact gggagcactt caatgccctc gatcgcgccg ggctgatgat
     1321 gtacgggcag atgaccgccg gctcgt
//
LOCUS       DAOQJH010000737         1345 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_101253_length_1345_cov_3.921705, whole genome shotgun
            sequence.
ACCESSION   DAOQJH010000737 DAOQJH010000000
VERSION     DAOQJH010000737.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1345
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101253_length_1345_cov_3.921705"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..462)
                     /locus_tag="PLQ18_13760"
     CDS             complement(<1..462)
                     /locus_tag="PLQ18_13760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011533438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HNE02768.1"
                     /translation="MDFLPLFLRLTGRPALVVGGGEVAARKVALLLDAGAEVRVVAPE
                     LGTTLAGEYAAGRIRHVARHFAAPDLDGADLVIAATDDVVVNAEVSRLARARSLPVNV
                     VDNPALCSFIMPAIVDRSPVIVALSTGGASPVLARLLRGRLESLIPAAYGKL"
     gene            604..>1345
                     /locus_tag="PLQ18_13765"
     CDS             604..>1345
                     /locus_tag="PLQ18_13765"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HNE02769.1"
                     /translation="MNARELAANVTELTTLPDVYQQIRALADDPHASLKQFADVVSMD
                     AAISARLLRLVNSPFYGLPGKVDSVLRAINLIGTDELCNLVLASSIGGMFKGVSNAAL
                     DLPAFWWRNVSVAMLARQLGRQNHMPSHERLFIAGILHDVGRLVAFQQVPTLAAEIER
                     TQTDAYDTGRQRAVLGFGYAELGAELLHQWGMPEALVEIVGCQPQPQRALEYGRDAAV
                     LHIAARAVPLIAADPGTPAELETLVAEDL"
BASE COUNT          216 a          483 c          468 g          178 t
ORIGIN      
        1 gagcttcccg taggcggccg ggatcagcga ttcgagccgc ccgcgcagca gccgcgccag
       61 caccggcgag gcaccaccgg tcgacagcgc gacgatcacc ggcgagcgat cgacgatcgc
      121 cggcatgatg aagctgcaga gcgccgggtt gtcgacgacg ttgaccggca gcgaacgcgc
      181 gcgcgccagc cgcgagacct cggcgttgac gaccacgtcg tcggtggcgg cgatgaccag
      241 atcggcgccg tcgagatcgg gcgcggcgaa atggcgtgcg acgtggcgga tgcggccggc
      301 ggcgtactcg ccggcgagcg tcgtgccgag ttccggtgcg acgacgcgca cctcggcacc
      361 ggcatcgagc agcagcgcca ccttgcgcgc ggccacctcg ccgccgccga cgaccagcgc
      421 cgggcggccg gtcaggcgca ggaacagggg caggaaatcc acgacgacac ctcggggctg
      481 gggggcagga aggacgcgac ttgtagcacc gtgcaacgaa tgctgccggg cgcgcacgcg
      541 actgtcaggg gccggtatgg tgtgcttaga taccgccccc cgtcacgccg aaggtcgccg
      601 aagatgaacg cgcgcgagct cgccgccaac gtcaccgaac tgaccacgct gcccgatgtg
      661 taccagcaga tccgggcact ggccgatgat ccgcatgcct cgctcaagca gttcgccgac
      721 gtggtcagca tggatgccgc gatttcggcg cggctgctgc gcctggtcaa cagtccgttc
      781 tacggcctgc ccggcaaggt cgacagcgtg ctgcgcgcga tcaacctgat cggcaccgat
      841 gagctctgca acctcgtgct cgccagctcg atcggcggca tgttcaaggg cgtcagcaat
      901 gccgcgctcg acctgccggc gttctggtgg cgcaacgtca gcgtcgcgat gctcgcgcgc
      961 cagctcgggc ggcagaatca catgccgtcc cacgagcgcc tgttcatcgc cggcatcctg
     1021 cacgatgtcg gccggctcgt ggccttccag caggtgccga cgctcgccgc cgagatcgag
     1081 cgcacgcaga ccgacgccta cgacaccggc cgccagcgcg ccgtgctcgg cttcggctac
     1141 gccgaactcg gcgccgaact gctgcaccag tggggcatgc cggaggcgct ggtcgagatc
     1201 gtcggctgcc agccgcagcc gcagcgcgcg ctcgaatacg gccgtgatgc cgccgtgctg
     1261 cacatcgccg cccgtgccgt gccgctgatc gcggccgacc ccggcacgcc ggccgagctg
     1321 gaaaccctgg tcgccgagga cctct
//
LOCUS       DAOQJH010000738         1342 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_101679_length_1342_cov_4.083916, whole genome shotgun
            sequence.
ACCESSION   DAOQJH010000738 DAOQJH010000000
VERSION     DAOQJH010000738.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1342)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1342)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1342
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101679_length_1342_cov_4.083916"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..713)
                     /locus_tag="PLQ18_13770"
     CDS             complement(<1..713)
                     /locus_tag="PLQ18_13770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012703500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase small subunit"
                     /protein_id="HNE02770.1"
                     /translation="MAEIETFYEVMRRQGITRRSFLKFCSLTAAALGLSPARAAEIAA
                     AMEQKPRTPVLWLHGLECTCCSESFIRAGHPLAKDVVLSMLSLDYDDTLMASAGHHAE
                     AILEEVMTKYKGNYILAVEGNPSLNMDGMSCIVGGKPFVEQLKHVAKDCKAIIAWGAC
                     ASWGCVQAARPNPTQAVPVHKVITDKPIIKVPGCPPIAEVMTGVITYMLTFDRLPELD
                     RQGRPKMFYGQRIHDKCYRR"
     gene            780..911
                     /locus_tag="PLQ18_13775"
     CDS             780..911
                     /locus_tag="PLQ18_13775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02771.1"
                     /translation="MILRQQKPGLPFRFNDLPNACRNRASVAGVNHATAAETCFPSE"
     gene            930..>1342
                     /locus_tag="PLQ18_13780"
     CDS             930..>1342
                     /locus_tag="PLQ18_13780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008063432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupU protein"
                     /protein_id="HNE02772.1"
                     /translation="MPKPWSVLWLQSGGCGGCSMALSCAEPPGLYARLAAFGIELLWH
                     PSLSEASGAEVIALLEAIAAGEHPLDCLCIEGALLRGPNGSGRFHVLGGTGVPTIDWV
                     RRLAARARCTVAVGSCAVSGGFSACAPNPTEACGL"
BASE COUNT          196 a          448 c          460 g          238 t
ORIGIN      
        1 cggcggtagc acttgtcgtg gatgcgctgg ccgtagaaca tcttcggccg gccctggcgg
       61 tcgagttcgg gcaggcggtc gaaggtcagc atgtaggtga tgacgccggt catgacctcg
      121 gcgatcggcg ggcagcccgg caccttgatg atcggcttgt cggtgatgac cttgtgcacc
      181 ggcacggcct gcgtcgggtt cggccgcgcc gcctgcacgc agccccagga cgcgcacgcg
      241 ccccaggcga tgatggcctt gcagtccttc gcgacgtgct tcagctgctc gacgaagggc
      301 ttgccgccga cgatgcagct catgccgtcc atgttcagcg acggattgcc ttcgacggcg
      361 aggatgtagt tgcccttgta cttcgtcatc acctcttcga ggatcgcctc ggcgtgatga
      421 ccggcggagg ccatcagcgt gtcgtcgtaa tcgagcgaca gcatcgacag cacgacatcc
      481 ttggcgagcg gatggccggc gcggatgaag ctttccgagc agcaggtgca ttcgagtccg
      541 tgcagccata acaccggcgt gcgcggcttc tgctccatcg ccgcagcgat ctcggccgcc
      601 cgggccggac tcaggccgag cgcggcggcg gtcaggctgc agaacttgag gaagctgcgc
      661 cgggtgatgc cctggcgccg catgacctca tagaaggttt cgatctcggc catgtatctt
      721 ccccccgtgg tcggcctgtc gttgtatcgg ggaaagacca tgctcaagga tcgtgccaga
      781 tgatcctgcg ccagcagaag cctggcttgc cctttcgttt caatgatctg ccgaatgcct
      841 gccgcaaccg ggcgtccgtg gccggcgtaa accacgcaac cgctgcggaa acctgctttc
      901 catccgagtg accgccggag gccgcaccga tgccgaaacc ctggagcgtg ctgtggctgc
      961 agtccggcgg ctgcggcggc tgttcgatgg cgctgagctg cgccgagccg cccggcctgt
     1021 acgcgcggct cgcggccttc ggcatcgagc tgctgtggca tccgtcgctg tcggaagcga
     1081 gcggtgccga ggtcatcgcg ctgctcgaag ccatcgccgc cggcgaacac ccgctcgatt
     1141 gtctctgcat cgaaggcgcg ctgctgcgcg gaccgaatgg cagcgggcgc ttccacgtgc
     1201 tcggcggcac cggcgtgccg accatcgact gggtgcgcag gctcgcggcg cgcgcccgct
     1261 gcacggtcgc ggtcggcagc tgcgcggtct cgggcggctt cagcgcctgc gcgccgaacc
     1321 cgaccgaagc ctgcggcctg ca
//
LOCUS       DAOQJH010000739         1341 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_101867_length_1341_cov_2.744946, whole genome shotgun
            sequence.
ACCESSION   DAOQJH010000739 DAOQJH010000000
VERSION     DAOQJH010000739.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1341
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101867_length_1341_cov_2.744946"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..666)
                     /locus_tag="PLQ18_13785"
     CDS             complement(<1..666)
                     /locus_tag="PLQ18_13785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNE02773.1"
                     /translation="MTAPAELPMPVPPLPASADLLRVRYGRTTIDGLSVFHRSAGDPA
                     APAFVLLHGFPSSSHMFRELIPRLAGRFHVIAPDYIGFGHSDAPPVERFEYRFDRLAG
                     IVATLLDRLGVGRYHLYMQDYGGPVGMRLATAAPGRVLGLVFQNANAYLDGIGAPARE
                     VFLPLWERGDETGARQMLTAEMTRWQYTAGARDPHGLNPDAWLHDQAGLDRHGSSERQ
                     LALF"
     gene            857..>1341
                     /locus_tag="PLQ18_13790"
     CDS             857..>1341
                     /locus_tag="PLQ18_13790"
                     /inference="COORDINATES: protein motif:HMM:NF018984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABATE domain-containing protein"
                     /protein_id="HNE02774.1"
                     /translation="MRIGEHPALDFANTLAAPAEVLLDFLATPAALLDWLAGTPELAD
                     AHALAQQAGARGELDAALAAVQALRAAWRSALPRLADAGARAALQETLNLWLAAGRGH
                     WQLVAQDGALRLLHVTDYTGPDSVAAAIAAVLAGLLAEVPATEVRKCENPACVLWFRD
                     T"
BASE COUNT          208 a          456 c          491 g          186 t
ORIGIN      
        1 aaacagcgcg agctggcgtt cgctgctgcc gtggcggtcg agcccggcct gatcgtgcag
       61 ccaggcgtcg gggttcaggc cgtgcggatc gcgggcgccg gccgtgtact gccagcgcgt
      121 catttccgcc gtcagcatct gccgggcgcc ggtctcgtcg ccgcgctccc acagcggcag
      181 gaacacttcg cgcgccggcg cgccgatgcc gtcgagatag gcgttggcgt tctggaacac
      241 caggccgagc acgcgcccgg gtgcggcagt cgccagccgc atgccgaccg ggccgccgta
      301 gtcctgcatg tacaggtgat agcgcccgac gccgaggcgg tcgagcagcg tcgcgacgat
      361 gccggcgagg cggtcgaagc ggtactcgaa gcgttcgacc gggggcgcat cggagtggcc
      421 gaagccgatg tagtcgggcg cgatgacgtg gaaacgcccg gccagacgcg ggatcagctc
      481 gcggaacatg tgcgaggacg acggaaagcc gtgcagcagc acgaaggccg gcgcggccgg
      541 atcgccggcg ctgcggtgga acacggacag gccgtcgatc gtcgtgcgcc cgtagcgcac
      601 gcgcagcaga tcggcggacg ccggcagcgg cgggacaggc atcggcagtt cggcaggggc
      661 ggtcatgatc gggaagctcc ggtgggggaa ggttggtaac ctttaaaatc taatttgatg
      721 gttactgaga cacaccgtag ccatgactcc aaaacctgtc aacatggttt ttatcggtta
      781 ctattccctc atgcccgacc cgaacgatgt tccgcccccc cctgagacac agccggcccc
      841 ggccgctctg gcgccgatgc gcatcggcga gcacccggcg ctcgacttcg ccaacacgct
      901 ggcggcgccg gccgaggtgc tgctcgattt cctcgccacg ccggcggcgc tgctcgactg
      961 gctggccggc acgccggaac tcgccgatgc gcacgcgctg gcgcagcagg ccggagcgcg
     1021 cggcgaactc gacgccgcac tggccgcggt gcaggcgctg cgcgcggcat ggcgcagcgc
     1081 gctgccgcga ctggcggatg ccggagcgcg cgcggcgctg caggaaaccc tgaacctctg
     1141 gctcgcggcc ggacgcggtc actggcagct cgtcgcgcag gacggcgccc tgcggctgct
     1201 gcacgtgacc gactacaccg ggccggacag cgtcgccgca gcgatcgccg ccgtgctcgc
     1261 cggactgctc gcggaggtgc cggcaaccga ggtgcgcaag tgcgagaatc cggcctgcgt
     1321 gctgtggttt cgcgatacca g
//
LOCUS       DAOQJH010000740         1325 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_104373_length_1325_cov_3.167717, whole genome shotgun
            sequence.
ACCESSION   DAOQJH010000740 DAOQJH010000000
VERSION     DAOQJH010000740.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1325
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104373_length_1325_cov_3.167717"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            138..878
                     /locus_tag="PLQ18_13795"
     CDS             138..878
                     /locus_tag="PLQ18_13795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010959923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HNE02775.1"
                     /translation="MLKPLFAALAIALGTATAAAGEATPAAGPDLTRLSAVLDGRKPD
                     AVNPTPVPGLYEVILGARVLYVSADGRYVLRGDLVDIDKGENLTEARSGQLRVRAIAA
                     VPESDMIVFAPPGPVQAKHTVTVFTDIDCGYCRKLHSEIDNYLRAGIRVRYLAYPRSG
                     LRGESYDKAVSVWCAEDPKAAITSAKKGQAVAKASCPDNPVAKEYELGNQVGVQGTPT
                     IVLENGDLVPGYVPAARLVMMLDHPELR"
     gene            complement(972..>1325)
                     /locus_tag="PLQ18_13800"
     CDS             complement(972..>1325)
                     /locus_tag="PLQ18_13800"
                     /inference="COORDINATES: protein motif:HMM:NF025912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNE02776.1"
                     /translation="ILRKLPEELQRMDPVPELLARLEVAEAAPKGIDTVPLEARLAAD
                     PNDHAARLELAEALLGASEHEGAAAQYLELMRRSRAFGDDAGRQGLLKLFELLGNEHP
                     VTGTFRRRMASLLLN"
BASE COUNT          193 a          459 c          480 g          193 t
ORIGIN      
        1 ggcgggccgt cgcccgcggc cgtcgttcga ccgatgcatc cgcggcaggg ctgccctgcg
       61 tatgtgccgg gtgcgcgggc gcaggccgcg cgttacgctg ccgcccttcc ccggatgacc
      121 cgatacggaa tcccgccatg ctgaaacccc tgttcgccgc cctcgcgatc gccctgggta
      181 cggcgaccgc tgccgccgga gaggccacgc ccgcggccgg tcccgacctg acgcggctca
      241 gtgccgtgct cgacggccgc aagcccgatg ccgtgaaccc gacgccggtg ccgggcctgt
      301 acgaggtcat cctcggggcg cgcgtgctgt acgtcagcgc cgatggccgc tacgtgctgc
      361 gcggcgatct ggtcgacatc gacaagggcg agaacctgac cgaggcacgc agcggccagc
      421 tgcgcgtgcg ggcgatcgcc gcggtgccgg aatccgacat gatcgtgttc gcgccacccg
      481 gtccggtgca ggccaaacat acggtgacgg tgttcaccga catcgactgc ggctactgcc
      541 gcaagctgca ttcggagatc gacaactacc tgcgcgccgg catccgcgtg cgctatctcg
      601 cctatccgcg cagcggcctg cgcggcgagt cctatgacaa ggccgtcagc gtctggtgtg
      661 ccgaggaccc gaaggcggcg atcaccagtg ccaagaaggg ccaggcggtc gcgaaggcga
      721 gctgcccgga caacccggtc gcgaaggaat acgaactcgg caaccaggtc ggcgtgcagg
      781 gcacgccgac catcgtgctc gaaaacggtg atctggtgcc cggctacgtg ccggcggcgc
      841 ggctggtgat gatgctcgac catcccgagc tgcgctgagg cgcggacagg ccgcggataa
      901 ccggccggca cgaaaacggg gcctcgcggc cccgttttca tcgggttgac ttcatcgggc
      961 tgacggcggg atcagttcag cagcagcgag gccatgcgcc ggcggaaggt gccggtgacc
     1021 gggtgctcgt tgccgagcag ctcgaacagc ttcagcaggc cctgacgccc ggcgtcgtcg
     1081 ccgaaggcgc ggctgcggcg catcagttcg aggtattgcg cggcggcgcc ctcgtgctcg
     1141 ctggccccga gcagcgcctc ggccagttcg agacgggcgg cgtggtcgtt cgggtcggcg
     1201 gcgagccggg cttcgagcgg cacggtgtcg atgcccttcg gcgcggcctc ggcgacttcg
     1261 agccgcgcga gcagctccgg caccgggtcc atgcgctgca gctcctcggg gagcttgcgc
     1321 aggat
//
LOCUS       DAOQJH010000741         1319 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_105310_length_1319_cov_4.512658, whole genome shotgun
            sequence.
ACCESSION   DAOQJH010000741 DAOQJH010000000
VERSION     DAOQJH010000741.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1319)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1319)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1319
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105310_length_1319_cov_4.512658"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..232)
                     /locus_tag="PLQ18_13805"
     CDS             complement(<1..232)
                     /locus_tag="PLQ18_13805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018718171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HNE02777.1"
                     /translation="MIRISKETDYGIVLLSCLAQAADASLSASALAERGRLPLPTVSK
                     VLKLLARGGLLVSQRGARGGYALARPPREITVA"
     gene            703..1230
                     /locus_tag="PLQ18_13810"
     CDS             703..1230
                     /locus_tag="PLQ18_13810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017991937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HNE02778.1"
                     /translation="MRPALPARLALLAALATALVACTPEAPLRPRPPFESADRILIEK
                     AARRLTLYSGERTLRSYRVSLGFAPVGPKRQEGDGKTPEGHYRIDWRNPDSRFHRSLH
                     VSYPDAGDVARAAARGVSPGGLIMIHGLPNGFGALGDWHRTRDWTDGCIAVTNDEMDE
                     IWALVADGTPIEIRP"
BASE COUNT          215 a          476 c          452 g          176 t
ORIGIN      
        1 cggcaaccgt gatctcgcgc ggcgggcgcg ccagagcgta gccgccacgt gcgccgcgct
       61 gcgagaccag cagcccgccg cgtgccagca gtttcagcac cttgctgacg gtcggcagcg
      121 gcagccggcc gcgttcggcg agcgcactgg cactgaggga agcgtccgcg gcctgcgcga
      181 gacaggacag cagcacgatg ccgtagtcgg tctccttgct gatgcggatc atggacgccc
      241 cgtaatcagc accggaaagg tgctatatgg gcgcagcata cgactgcaag caggactagg
      301 gaagtcctca tttcggggaa gacgcggcag acgcatggag cagcccggaa aacgggccgc
      361 tgcgtccggc atcgatgccc gcgggcgcgg gcgaaggaag gcaggaagtg cgcgggctca
      421 ggcccgcggc agcgcggtgt cgttcagtgg cgcagccagc tccagcactg gcgcagcagt
      481 ccccacagac tctggcgggc atgggcagga ccaccgtgcc ggcgcagcga ggcaagctgg
      541 ttgcgggtcg cgtaactcat ctctcggctc cggcaggctg actgatgagt cgatgataat
      601 cattatcgaa tacgaatcac agccgaatct gtgcggctgt ccggccggtc acggactgcg
      661 cacaatgccc tcccgctcca cccgcccgga ggaaaccgac cgatgcgccc cgccctgccg
      721 gcccgcctgg ccctgctcgc cgccctggcc acggccctcg tcgcctgcac gccggaagcc
      781 ccgctgcggc cgcgtccgcc gttcgagtcc gccgaccgca tcctgatcga gaaggccgcg
      841 cggcggctga cgctgtattc gggcgagcgg acgctgcgca gctaccgcgt ctcgctcggc
      901 ttcgcgccgg tcggtccgaa acgccaggaa ggcgacggca agacccccga aggccattac
      961 cggatcgact ggcgcaaccc cgacagccgc ttccaccgct cgctgcacgt gtcgtatccg
     1021 gacgccggcg atgtcgcgcg agccgcggcg cgcggcgtgt cgccgggcgg gctgatcatg
     1081 atccacggcc tgccgaacgg cttcggcgcg ctcggtgact ggcaccggac gcgcgactgg
     1141 accgacggct gcatcgccgt caccaatgac gagatggacg aaatctgggc actggtcgcc
     1201 gacggcacgc cgatcgagat ccggccctag tccaccgcct gtcggccgcc ccggtcggcc
     1261 ggtaccgatc gccgcccccc gcttacccgc cccggacgat accggcgccg gtaagcggg
//
LOCUS       DAOQJH010000742         1317 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_105652_length_1317_cov_2.816957, whole genome shotgun
            sequence.
ACCESSION   DAOQJH010000742 DAOQJH010000000
VERSION     DAOQJH010000742.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1317
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105652_length_1317_cov_2.816957"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1317)
                     /locus_tag="PLQ18_13815"
     CDS             complement(<1..>1317)
                     /locus_tag="PLQ18_13815"
                     /inference="COORDINATES: protein motif:HMM:NF024516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3971 domain-containing protein"
                     /protein_id="HNE02779.1"
                     /translation="LARLQPSLHIPQARLRFDPAHPDAWAAVALVEDLRVHADGAVPG
                     IDRLDGTLQLTATHGRLDFDAPWLSFEASAVFRSPFFFETARGRLDWMQTGDGGWRID
                     IGRLELATPGIAAIVTGSVTLPAGGGAPYLDLSAEVPRLQVGAAPTYLPDQVIGEGGM
                     DWLDRGLVAGSGYDGHVRLRGRADGFPYRDGDGEFEARFRVEDTTLDFAPGWPRLENL
                     EAGVVFAGPGLTVQAESATLLGVPVTAEVRIADLEHTLVEVDGVSRADGAQLQRILRE
                     TPVRARLGPFAEAMTLGGPHTVDLALRIPTDGRPMDGRGRIHFLGGTLDLPDAGWHFT
                     HLHGALDFDDGSLSAENLGLLFGDEPMRLDVASTGGAGNGETVFRLAGRYRLQTLLGE
                     AADELSGSGRDALASGETDGVLTLTVPAARAGAPQYVLGFDSPLSG"
BASE COUNT          183 a          485 c          490 g          159 t
ORIGIN      
        1 ggccgctcag cggactgtcg aagccgagca cgtactgcgg cgcgccggcg cgcgcggccg
       61 gcacggtcag cgtcagcacg ccgtcggtct cgccgctcgc gagcgcatcg cggccgctgc
      121 cggacagctc gtcggcggcc tcgccgagca gcgtctgcag ccggtagcgg ccggcgagcc
      181 ggaacaccgt ctcgccgttg ccggcgccgc cggtgctggc gacatcgagg cgcatcggct
      241 cgtcgccgaa cagcaggccg aggttctcgg cgctcaggct gccgtcgtcg aaatcgagcg
      301 cgccgtgcag gtgcgtgaaa tgccagccgg catcgggcag atcgagcgtg ccgccgagga
      361 agtggatgcg gccgcgcccg tccatcggcc ggccatcggt cgggatgcgc agcgccagat
      421 cgacggtgtg cggcccgccg agcgtcatcg cctcggcgaa gggtccgagg cgcgcgcgca
      481 ccggcgtctc gcgcaggatg cgctgcagct gcgcgccatc ggcacggctg acgccgtcga
      541 cctcgaccag cgtgtgttcg agatcggcga tgcgcacttc ggccgtgacc ggcacgccga
      601 gcagcgtcgc cgattcggcc tgcaccgtca ggcccggacc ggcgaacacg accccggctt
      661 cgaggttttc gagccgcggc cagccgggcg cgaagtcgag cgtcgtgtcc tcgacgcgga
      721 agcgcgcctc gaactcgccg tcgccgtcgc ggtacgggaa gccgtcggcg cggccgcgca
      781 ggcgcacgtg gccgtcgtag ccgctgccgg caaccagccc gcgatcgagc cagtccatgc
      841 cgccttcgcc gatgacctga tccggcagat aggtcggcgc agccccgacc tgcaggcgcg
      901 gcacctcggc gctgaggtcc agatacggcg caccgccgcc ggccggcagc gtcaccgagc
      961 cggtgacgat cgcggcgatg ccgggggtcg cgagttcgag gcgtccgatg tcgatgcgcc
     1021 agccgccatc gccggtctgc atccagtcga ggcgcccgcg cgcggtctcg aagaagaacg
     1081 gcgagcggaa caccgcgctc gcctcgaagc tcagccacgg cgcgtcgaaa tcgagccgcc
     1141 cgtgcgtcgc cgtgagctgc agcgtgccgt cgagccggtc gatgccgggc acggcgccat
     1201 cggcgtgcac gcgcaggtcc tcgaccagcg ccaccgccgc ccaggcgtcg gggtgcgccg
     1261 gatcgaagcg caggcgggcc tgcgggatgt gcagcgaagg ctgcagccgc gcaagcc
//
LOCUS       DAOQJH010000743         1312 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_106447_length_1312_cov_2.800318, whole genome shotgun
            sequence.
ACCESSION   DAOQJH010000743 DAOQJH010000000
VERSION     DAOQJH010000743.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1312
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106447_length_1312_cov_2.800318"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            251..766
                     /locus_tag="PLQ18_13820"
     CDS             251..766
                     /locus_tag="PLQ18_13820"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNE02780.1"
                     /translation="MRRSVLAATLLFTSALAGAEPVDLTLPTTDGSTLSLSSLRGRWV
                     VVNVWATWCSPCIKELPELAAFHAEHKSKDATVVGVAYENTTAGMLSKFIEPYKLGYP
                     VMMAGTTPLPGFPKMKGVPTTFILNPKGELVRTYTGPVTAAGVELLIDDLREPAKPAA
                     AIHDTPARPAT"
     gene            774..1061
                     /locus_tag="PLQ18_13825"
     CDS             774..1061
                     /locus_tag="PLQ18_13825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006474297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HNE02781.1"
                     /translation="MHRSAAAVICRRFHVSGRVQGVSFRAYTQARALELGLSGYARNL
                     PDGRVEVLACGSEPAVGQLHDWLWTGSPAAQVESVTVSEALFEAHAGFDRG"
BASE COUNT          209 a          460 c          422 g          221 t
ORIGIN      
        1 gccggcggcg ccgcgcggca tcttagcggc atccgccgca gccgtgatcc gcaaaccggg
       61 cgtcgaacct ttgcagcggt cggcccgtca gtggctgaag caggtgccgg ctcgtcttcc
      121 ggggtggcgc ctgtggtcgg gcccgccgcc ggtgcccgac gcgcactggc acgctgatcg
      181 tgcgcgccgc cgccggacgc aaggctgcgc taggattaca aaaccttcat tcgtttgagg
      241 agaagctgtc gtgcgccgat ccgtcctcgc cgccacgctg ctgttcacgt cggccctcgc
      301 cggtgccgaa cccgtagacc tgaccctgcc gaccacggat ggctcgacgc tcagcctgtc
      361 ctcgctgcgc ggccgctggg tggtcgtcaa cgtctgggcg acctggtgca gcccctgcat
      421 caaggaactg ccggaactgg ccgccttcca cgccgagcac aagagcaagg atgcaacggt
      481 ggtcggcgtc gcctatgaga acaccacggc cggcatgctt tcgaagttca tcgagccgta
      541 caagctcggc tatccggtca tgatggccgg caccacgccg ctgccgggct tcccgaagat
      601 gaagggtgtg ccgacgacct tcatcctcaa tccgaagggc gagctggtgc gcacctacac
      661 gggtccggtc accgccgccg gcgtcgaact gctgatcgat gacctgcgcg agccggccaa
      721 gccggccgct gccatccacg acaccccggc ccggccggcc acctgagtcc cccatgcacc
      781 gatccgctgc cgccgtgatc tgccgccgtt tccatgtctc cgggcgcgtg cagggcgtgt
      841 cgttccgcgc gtacacgcag gcacgtgccc tcgaactcgg cctgagcggc tacgcccgca
      901 acctgccgga tggccgggtc gaggtgctgg cctgcggcag cgaaccggcc gtcggccagc
      961 tgcacgactg gctgtggacc ggctcgccgg ctgcgcaggt cgaatcggtg acggtcagcg
     1021 aagcgctgtt cgaggcgcac gcaggcttcg atcgcggctg aatgccggtc tcgtggtcga
     1081 gccggccgca gcatcgggac ttgacttatg cacaaagccc tgcacagaca ccgcatctga
     1141 cgcaggcgga cactgcctcg cagaatgatg cgtcaaggtc ttgatttcac tacattgatc
     1201 aactacgccg cagccccgtc ggccacgtca aaaagcctga acgggcatgg gcttggcgtt
     1261 ttgtccccga agttttccac aggcttgtcc acggcttctg tggacaagca gg
//
LOCUS       DAOQJH010000744         1300 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_108375_length_1300_cov_3.627309, whole genome shotgun
            sequence.
ACCESSION   DAOQJH010000744 DAOQJH010000000
VERSION     DAOQJH010000744.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1300)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1300)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1300
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108375_length_1300_cov_3.627309"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..460)
                     /locus_tag="PLQ18_13830"
     CDS             complement(<1..460)
                     /locus_tag="PLQ18_13830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006477109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bc complex cytochrome b subunit"
                     /protein_id="HNE02782.1"
                     /translation="MANHPHPGTTGLLGWVDARFPATKLWREHLSEYYAPKNFNFWYF
                     FGSLALLVLVNQILTGIWLTMSYKPSATEAFASVEYIMRDVDWGWLIRYMHSTGASAF
                     FIVVYLHMFRGLIYGSYKQPRELIWIFGVLIFLALMAEAFMGYLLPWGQMS"
     gene            complement(460..1056)
                     /gene="petA"
                     /locus_tag="PLQ18_13835"
     CDS             complement(460..1056)
                     /gene="petA"
                     /locus_tag="PLQ18_13835"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007041461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045275 - respiratory chain complex III
                     [Evidence IEA];
                     GO_function: GO:0008121 - ubiquinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HNE02783.1"
                     /translation="MSTADGVNLGRRRFLTATATAVGGVGAAFVAVPFLKSWTPSERA
                     KAAGAPVEADISKLAEGQIMTVEWRGKPVWVVHRSKAMLDALPALDGKMSDPKSEMDQ
                     QPAYAKNEFRSIKPEYLVLVGICTPLGCSPTFRPDLAPADLGAEWKGGFFCPCHGSRF
                     DLAGRVYSGVPAPKNMVVPPYKYLSDTRILVGVGAEAA"
BASE COUNT          255 a          401 c          424 g          220 t
ORIGIN      
        1 acgacatctg gccccagggc agcaggtagc ccatgaaggc ctcggccatc agcgcgagga
       61 agatcagcac gccgaagatc cagatcagtt cgcgcggctg cttgtacgag ccgtagatca
      121 ggccgcggaa catgtgcagg tagacgacga tgaagaacgc cgacgcaccg gtcgagtgca
      181 tgtagcggat cagccagccc cagtcgacgt cgcgcatgat gtattcgacg gacgcgaagg
      241 cttcggtcgc cgagggcttg tagctcatcg tcagccagat gccggtcagg atctggttga
      301 ccagcaccag cagcgccagc gagccgaaga agtaccagaa gttgaagttc ttcggcgcgt
      361 aatactcgga caggtgctcg cgccagagct tggtcgccgg aaagcgcgca tcgacccagc
      421 cgagcaggcc ggtcgtaccc ggatgtggat gattcgccat tacgcagcct ccgcgccgac
      481 gccgaccaga atgcgggtgt cgctcaggta cttgtacggg ggaacgacca tgttcttcgg
      541 cgccggcacg ccgctgtaga cacgcccggc gaggtcgaag cgggaaccgt ggcacggaca
      601 gaagaagccg cccttccatt cggcaccgag gtcggcgggt gcgaggtcgg gccggaaggt
      661 cggcgagcag ccgaggggtg tgcagatgcc gaccagcacc agatattcgg gcttgatcga
      721 gcggaactcg ttcttggcgt aggccggctg ctggtccatc tcggacttcg gatcggacat
      781 cttgccgtcg agcgcgggca gcgcatcgag catggccttg ctgcgatgca cgacccacac
      841 cggcttgccg cgccattcga ccgtcatgat ctggccttcg gcgagcttgc tgatgtcggc
      901 ctcgaccggc gcgcccgccg ccttggcccg ttcgctcggt gtccaggact tcaggaaggg
      961 caccgccacg aacgctgcgc cgacgccacc gacggcggtg gcagtggcgg tcaggaaccg
     1021 ccgcctgccg aggttcactc cgtctgccgt actcatacgc tctctcctaa cagactgaaa
     1081 aaaattgatt ttgaccggtt tatcgagcgt cccgaccggt ctgcggaggc cacggcggca
     1141 gccgcccgcg agctgaaccg ccggccgcaa ctaaacgcgg gatgttcgta taacgtttcg
     1201 taagaatcaa gacgaaaccc ttatttcact tgaggaaaat caagggttgc gggcgtgcac
     1261 agcgtggctg ttgccgccac ttctgcaagg cgggattcag
//
LOCUS       DAOQJH010000745         1297 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_108865_length_1297_cov_4.088567, whole genome shotgun
            sequence.
ACCESSION   DAOQJH010000745 DAOQJH010000000
VERSION     DAOQJH010000745.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1297)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1297)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1297
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108865_length_1297_cov_4.088567"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..594)
                     /locus_tag="PLQ18_13840"
     CDS             complement(<1..594)
                     /locus_tag="PLQ18_13840"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNE02784.1"
                     /translation="MSVTDSSPPEDRRQYFRVADEVVLAWARVDGDGLAALAERLRGE
                     GSEGYGFGPLYAALTQDAQGLLAQLRSEQPLVAQYTENLERRLDILASAIVLADMGVH
                     ERSARHVNLSAGGIEFEAETALETDATLELRFVIHPNRSVIVAGARVARCLPADPRSA
                     WPWRIAVEFICLREQDRQMIVRHVMFREARHLRERQPF"
     gene            complement(669..1142)
                     /locus_tag="PLQ18_13845"
     CDS             complement(669..1142)
                     /locus_tag="PLQ18_13845"
                     /inference="COORDINATES: protein motif:HMM:NF016985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HNE02785.1"
                     /translation="MLTVLIEQELAATRALLAVLEQEYSALRERHDADTLEAVVRAKE
                     TAAGRLQQIAAGRMQHLLGSGYPPDAAGLAACIAAVPTEDRFRLNALWRELLDVAGRA
                     WKQNGINGAVIAASRSATERALALLRGQDPNTCLYGADARARYATGGSVRPLISA"
     gene            complement(1144..>1297)
                     /gene="flgM"
                     /locus_tag="PLQ18_13850"
     CDS             complement(1144..>1297)
                     /gene="flgM"
                     /locus_tag="PLQ18_13850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020295659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HNE02786.1"
                     /translation="KLSETPAFDSARVAELRKAIERGEYRVDSRRVAGKLLEFERDLS
                     APGAGD"
BASE COUNT          178 a          439 c          475 g          205 t
ORIGIN      
        1 gaagggctgg cgttcgcgca ggtgacgggc ttcgcggaac atcacgtggc gcacgatcat
       61 ctggcggtcc tgctcgcgca ggcagatgaa ttcgacggcg atgcgccacg gccaggcgct
      121 gcgcggatcg gcgggcaggc agcgggcgac gcgggcgccg gcgacgatca ccgagcggtt
      181 cgggtggatc acgaagcgca gctccagcgt cgcatcggtt tccagggccg tctcggcctc
      241 gaactcgatg ccgcccgccg acaggttgac gtggcgggcg ctgcgctcgt gcacgcccat
      301 gtcggcgagc acgatggcgc tcgcgaggat gtcgagccgg cgttcgaggt tctcggtgta
      361 ttgcgcgacc agtggctgtt cgctgcgcaa ctgcgcgagc agcccctgcg cgtcctgcgt
      421 cagcgccgcg tacagcggtc cgaagccgta gccttcgctg ccttcgccgc gcaggcgttc
      481 ggcgagtgcg gccagcccgt cgccatcgac gcgtgcccag gcgagcacga cttcatcggc
      541 gacgcggaaa tactgacggc gatcctcggg cggcgacgaa tctgtgacgc tcatcgactg
      601 gatgttccgg cggaaggggg cggcggatca tcgcatccgc tgccccgcgt cgtcatgcgc
      661 gacgggtttc aggcactgat cagcggccgc acgctgccac cggtggcata gcgggcgcgg
      721 gcgtcggcgc cgtacaggca ggtgttcggg tcctggccgc gcagcagcgc cagcgcgcgt
      781 tcggtggcgc tgcgactggc agcgatcacc gcgccgttga tgccgttctg cttccaggcg
      841 cgaccggcga catcgagcag ttcgcgccac agcgcgttca gccggaagcg atcctcggtc
      901 ggcaccgcgg cgatgcaggc tgcgaggccg gcggcatcgg gcggatagcc gctgccgagc
      961 aggtgctgca tgcgcccggc ggcgatctgc tgcagccgtc cggccgcggt ttccttggcg
     1021 cgcacgacgg cctcgagcgt gtcggcatcg tggcgttcgc gcagcgccga gtattcctgt
     1081 tcgagcacgg cgagcagggc gcgcgtcgcg gcgagttcct gctcgatcag cacggtcagc
     1141 atgtcagtct cctgcacccg gggcggacag gtcacgctcg aactccagca gcttgccggc
     1201 gacacgccgg ctgtcgacgc ggtattcgcc gcgttcgatc gccttgcgca gctccgccac
     1261 ccgcgccgaa tcaaaggccg gggtttcgga gagcttc
//
LOCUS       DAOQJH010000746         1296 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_109026_length_1296_cov_4.807413, whole genome shotgun
            sequence.
ACCESSION   DAOQJH010000746 DAOQJH010000000
VERSION     DAOQJH010000746.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1296)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1296)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1296
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109026_length_1296_cov_4.807413"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..115)
                     /locus_tag="PLQ18_13855"
     CDS             complement(<1..115)
                     /locus_tag="PLQ18_13855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016499826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AsnC family transcriptional regulator"
                     /protein_id="HNE02787.1"
                     /translation="MNVPDADKDRQLLALLSANAREPLASLARKLGVARSSL"
     gene            257..451
                     /locus_tag="PLQ18_13860"
     CDS             257..451
                     /locus_tag="PLQ18_13860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012314630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1272 domain-containing protein"
                     /protein_id="HNE02788.1"
                     /translation="MSLEMKPACERCRQPLPPDSPDARICSYECTYCRDCSEHRLHGR
                     CPNCGGELLARPRRLPTAGG"
     gene            complement(435..>1296)
                     /locus_tag="PLQ18_13865"
     CDS             complement(435..>1296)
                     /locus_tag="PLQ18_13865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012702530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methionine aminotransferase"
                     /protein_id="HNE02789.1"
                     /translation="EALFCAIQAVLRAGDEAIVFDPCYDSYEPGIELAGGRAVHVPLA
                     GPDFRPDWQRFAGALSPRTRLVILNTPHNPTGSVWSAEDMQQLATLLRGRGIWLLSDE
                     VYEHIVFDGRRHESVLLHPELAARAFVVSSFGKTFHATGWKIGYCIAPAALSAEFRKI
                     HQFVTFSTSTPMQHALADYLEAEAQHWQQLPAFYQRKRDLFCRLIEGSRFGCTPSAGT
                     YFQLLDYSAISDERDVDLARRLTREARLASIPVSVFSVAPDPAQRLLRFCFAKDDATL
                     EQAAAIIRRL"
BASE COUNT          203 a          458 c          460 g          175 t
ORIGIN      
        1 gcaggctgct gcgtgcgacg ccgagcttgc gcgcgaggct cgcgagcggt tcgcgggcat
       61 tcgcactgag cagcgccagc agctggcgat ccttgtcggc atcggggacg ttcatcggcg
      121 gcgacctggg cggcacgggg ggcttgcgcc ggccgcggcc ggcggatgtc gggggcggcc
      181 ggtcgatgcg ccgggtcgaa atccggccgt cccggcagcg agcatgccag cacctgccct
      241 gcccgaggaa ccatcgatgt cactcgagat gaaacccgcc tgcgaacgct gccggcagcc
      301 gctgccgccg gactcccccg acgcccgcat ctgttcgtac gaatgcacgt actgccgcga
      361 ctgcagcgag caccggctgc atggccgctg cccgaactgc ggcggcgagc tgctggcgcg
      421 gccgcgccgc ctgcctacag ccggcggatg atcgccgccg cctgttcgag cgtcgcgtcg
      481 tccttggcga agcagaagcg cagcagccgc tgcgccggat cgggcgcgac gctgaacacc
      541 gataccggaa tcgacgcgag ccgcgcctcg cgcgtcagcc gccgcgccag atcgacatcc
      601 cgctcgtcgc tgatcgccga atagtcgagc agctggaagt aggtgccagc cgagggcgtg
      661 cagccgaagc gcgaaccctc gatcagccgg cagaacagat cgcgcttgcg ctggtagaag
      721 gccggcagct gctgccagtg ctgcgcctcg gcctccaggt aatcggccag cgcgtgctgc
      781 atcggcgtcg aggtcgagaa ggtcacgaac tggtggatct tgcggaactc cgccgacagc
      841 gccgccggtg cgatgcagta gccgatcttc cagccggtgg catggaaggt cttgccgaac
      901 gaggacacga caaaggcccg cgccgccagc tccggatgaa gcagcacgct ctcgtgccgg
      961 cggccgtcga agacgatgtg ctcgtagacc tcgtcggaca gcagccatat cccgcgcccg
     1021 cgcagcagcg tcgcgagctg ctgcatgtcc tcggccgacc agacgctgcc ggtcgggttg
     1081 tgcggcgtgt tgaggatgac gagccgcgtg cgcggactca gcgccccggc aaagcgctgc
     1141 cagtccggcc ggaaatccgg ccccgccagc ggcacgtgca cggcccggcc gccggcgagc
     1201 tcgatgccgg gctcgtagct gtcgtagcag gggtcgaaca cgatcgcctc gtcgccggcg
     1261 cgcagcaccg cctggatcgc gcagaacagc gcctcg
//
LOCUS       DAOQJH010000747         1291 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_109948_length_1291_cov_3.550162, whole genome shotgun
            sequence.
ACCESSION   DAOQJH010000747 DAOQJH010000000
VERSION     DAOQJH010000747.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1291)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1291)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1291
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109948_length_1291_cov_3.550162"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="PLQ18_13870"
     CDS             <1..201
                     /locus_tag="PLQ18_13870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02790.1"
                     /translation="ASVLLHELVHYLQDLRGGFSDGSCRAGLLREVEAFRWQERWLAR
                     AGAWQPVGMALMNYGCATPAAG"
     gene            326..>1291
                     /locus_tag="PLQ18_13875"
     CDS             326..>1291
                     /locus_tag="PLQ18_13875"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HNE02791.1"
                     /translation="MIVLLLIFLNGLFAMSELAVVSARRARLQVLAEQGSKGAVAALK
                     LTEHPGRFLSTVQIGITLIGIFAGAYGGATLSEPLAAWIAEHHPALADSADTLALAIV
                     VALITYLSLIVGELVPKQLALRNPERIAVLAAPPMTVLAFLAAPIVHLLDLSANLGLR
                     LFGAEAQTEQKVTDEEIRTLLKEATEAGVVEHSEQAMIQGVMRLADRPVKALLTPRPD
                     IVWLDPADADAENIATLRASPYSRYPVCRGSVDEILGVVQAKDLLDDVLAGKPLDLRA
                     CLREAPVVHEDASALGLLETLRTSPLPLAFVVDEYGDLVGMVTATD"
BASE COUNT          181 a          474 c          436 g          200 t
ORIGIN      
        1 gcgagcgtgc tgctgcacga actcgtgcat tacctgcagg acctgcgcgg cggtttcagc
       61 gatggcagct gccgcgccgg gctgctgcgc gaggtcgagg ccttccgctg gcaggagcgc
      121 tggctggcgc gcgccggcgc ctggcagccg gtcggcatgg cgctgatgaa ctacggctgc
      181 gcgacgccgg cggccggatg agtcccgggg tccggcgcat cgccatgacc ggcgagcatg
      241 acgccgccag ccgcggcccg ctaagatcgc cggcgttgcc gtccggcaac gctgacgcca
      301 cccatcgatg attctctggg agctgttgat cgtcctgctg ctgatctttc tgaacggcct
      361 gttcgcaatg tcggaactcg cagtggtctc ggcccgccgg gcccggctgc aggtactcgc
      421 cgagcagggc agcaagggcg ccgtcgcggc gctgaaactc accgaacacc ccggccgctt
      481 cctgtccacc gtgcagatcg gcatcacgct gatcggcatc ttcgccggcg cctacggcgg
      541 cgcgacgctg tcggaaccgc tcgccgcgtg gatcgccgag catcatccgg cgctcgccga
      601 ctcggccgac acgctggcgc tcgccatcgt cgtggcgctg atcacctatc tgtcgctgat
      661 cgtcggcgaa ctcgtgccga agcagctggc actgcgcaat cccgagcgca tcgccgtgct
      721 cgccgcgccg ccgatgacgg tgctcgcctt cctcgccgcg ccgatcgtgc acctgctcga
      781 cctgtcggcc aacctcggcc tgcggctgtt cggcgccgag gcgcagaccg agcagaaggt
      841 caccgacgag gaaatccgca cgctgctcaa ggaagccacc gaggccggcg tcgtcgagca
      901 cagcgagcag gcgatgatcc agggcgtcat gcgcctggcc gaccggccgg tgaaggcgct
      961 gctgacgccg cgcccggaca tcgtctggct cgacccggcc gatgccgatg ccgagaacat
     1021 cgccacgctg cgcgcgagtc cgtactcgcg ctacccggtc tgccgcggtt cggtcgacga
     1081 gatcctcggc gtcgtgcagg ccaaggacct gctcgatgac gtgctcgccg gcaagccgct
     1141 cgacctgcgc gcctgcctgc gcgaggcgcc ggtcgtgcac gaggacgcga gcgccctcgg
     1201 cctgctcgaa accctgcgca cctcgccgtt gccgctcgcc ttcgtcgtcg acgaatacgg
     1261 cgatctggtc ggcatggtca cggccaccga c
//
LOCUS       DAOQJH010000748         1288 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_110464_length_1288_cov_4.777778, whole genome shotgun
            sequence.
ACCESSION   DAOQJH010000748 DAOQJH010000000
VERSION     DAOQJH010000748.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1288
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110464_length_1288_cov_4.777778"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            3..446
                     /locus_tag="PLQ18_13880"
     CDS             3..446
                     /locus_tag="PLQ18_13880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011506830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HNE02792.1"
                     /translation="MLQQLQAAGRQTWVELAAAVGLSPSPCLERVRRLEKDGVIRGYA
                     ALVEPSAVGASLLVFVEISLDYTTPDIFERFRAAVAGLPQIQECHLVSGNFDYLLKAR
                     IGDMAEYRELLGLFLRTLPGVRDSRSFVVMETVKETQAVFVPETG"
     gene            728..1057
                     /locus_tag="PLQ18_13885"
     CDS             728..1057
                     /locus_tag="PLQ18_13885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012356841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNE02793.1"
                     /translation="MRIRTLLLPALALGCALASLPAPAEPSPARQRELRHLVRQDCGS
                     CHGMTLKGGLGTPLLPSALAGKTDALLVATVLDGRPNTAMPPWRGLLTEAEAVWIVAQ
                     LRAGLPE"
     gene            1090..>1288
                     /locus_tag="PLQ18_13890"
     CDS             1090..>1288
                     /locus_tag="PLQ18_13890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02794.1"
                     /translation="MRTDPPVSVPAARQPADGRPRLRSLLLALAAIAAGLTGLARSAP
                     ADEAAVPGLRATGDLALIVERA"
BASE COUNT          166 a          487 c          434 g          201 t
ORIGIN      
        1 gcatcctgca gcagttgcag gccgccggcc gccagacctg ggtcgagctg gccgcggcgg
       61 tcgggctgtc gccgtcgccg tgtctggagc gcgtgcgccg gctggagaag gacggcgtca
      121 tccgcggcta cgcggcgctg gtggaaccct ccgcggtcgg tgccagcctg ctggtgttcg
      181 tcgagatcag cctcgactac acgacgcccg acatcttcga acgcttccgc gccgccgtcg
      241 ccggcctgcc gcagatccag gaatgccacc tggtttccgg caacttcgac tatctgctga
      301 aggcgcgcat cggcgacatg gccgaatacc gcgaactgct cggcctgttc ctgcgcacgc
      361 tgccgggcgt gcgcgattcg cgcagcttcg tggtgatgga gacggtcaag gagacgcagg
      421 cggtgttcgt gccggagacc gggtgaacgc ggccggcgac cggcagacac ccgtacccgc
      481 ttcaccgctt acagcgatca caggtaaacg catggttttt ctcccccgct cgccaacccc
      541 gcgcccacgc ggggcgaggg gctgaaaagc aaagcgttct gatgtgatcg gcattacctc
      601 ctgccctact ccctgctacc tcctctgccc gcccgccagt tccctgccgc ttgatgggta
      661 tcaagggcgc tggctgctcc ggcgcggtgc aatgcgcggc atctgcactg tcatggagcc
      721 gccgtccatg cgaatccgca ccttgctgct tcccgcgctc gcgctcggct gcgcgctggc
      781 atcgctgccg gcgccggccg aaccgtcgcc ggcgcgccag cgcgagcttc gccacctcgt
      841 gcggcaggac tgcggctcct gccacggcat gacgctgaag ggcgggctcg gcacgccgct
      901 gctgccctcg gcgctggccg gcaaaaccga tgcgctgctg gtcgcgaccg tgctcgacgg
      961 ccggccgaac acggcgatgc cgccgtggcg cgggctgctc accgaagccg aagccgtgtg
     1021 gatcgtcgcg cagctgcgcg ccggcctgcc cgaatgatcg aacgctcccc ctgcgtgagg
     1081 actgccgcga tgcgaaccga cccgcccgtc tccgttcctg ctgcccgcca gcccgccgac
     1141 ggccggccgc gactgcgctc gctgctgctc gcgctcgcgg cgatcgccgc cggcctgacc
     1201 ggcctggccc gcagcgcccc ggccgacgaa gccgcggtcc cggggctgcg cgccaccggc
     1261 gatctggcgc tgatcgtcga gcgcgccg
//
LOCUS       DAOQJH010000749         1282 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_111529_length_1282_cov_3.673187, whole genome shotgun
            sequence.
ACCESSION   DAOQJH010000749 DAOQJH010000000
VERSION     DAOQJH010000749.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1282
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111529_length_1282_cov_3.673187"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..983
                     /locus_tag="PLQ18_13895"
     CDS             <1..983
                     /locus_tag="PLQ18_13895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNE02795.1"
                     /translation="LPTVMTLMALGTLPETHLLQLGMLGMHGARCTNLALAECDLLIA
                     VGARFDDRATGKVAEFCPNAAIVHIDIDASELDKIKTAHVALNGDVREVLGRLLPRLG
                     AQADRRAWLARIGELKRDFPLKLDGRHNPRHHYGLIRATARAAGPAAIVTTDVGQHQM
                     WAAQAYPLDRPRQWLTSGGLGTMGFGLPAAIGAALAEPQRPVVCFSGDGSILMNIQEL
                     ATLAEEDLDVKIVLFNNGALGLVHQQQTLFYGGRLNASVFRGQPDFVRLAQAFGIRTI
                     DLDAEPDVPDALAAAFAERGPCLIHATIGLEEKVYPMVPPGAANTDMIGG"
     gene            987..>1282
                     /gene="ilvN"
                     /locus_tag="PLQ18_13900"
     CDS             987..>1282
                     /gene="ilvN"
                     /locus_tag="PLQ18_13900"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018606327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HNE02796.1"
                     /translation="MNATRTVPAALAVLELTVNNHPGVMSHVCGLFARRACNVEGIIC
                     LPLGDGARSRIWLQVPDTDKLEPMTRQARKLEDVLEVIRHPDGHPVFAQLGAAV"
BASE COUNT          201 a          478 c          420 g          183 t
ORIGIN      
        1 gcctgccgac cgtgatgacg ctgatggcgc tcggcacgct gccggaaacg cacttgctgc
       61 agctcggcat gctcggcatg cacggcgcgc gctgcacgaa tctcgcgctc gccgaatgcg
      121 acctgctgat cgcggtcggc gcgcgcttcg atgaccgcgc caccggcaag gtcgccgagt
      181 tctgcccgaa cgcggcgatc gtgcacatcg acatcgacgc gagcgaactc gacaagatca
      241 agaccgcgca cgtggcgctc aacggcgacg tgcgcgaggt gctcggccgg ctgctgccgc
      301 ggctcggcgc gcaggcggat cgccgtgcgt ggctggcgcg catcggcgag ctgaagcgcg
      361 acttcccgct gaagctcgac ggtcgccaca acccgcgcca ccactacggc ctgatccgcg
      421 cgacggcacg cgccgccggc ccggccgcga tcgtcaccac cgatgtcggc cagcaccaga
      481 tgtgggccgc gcaggcttat ccgctcgacc gtccgcgcca gtggctgacc tcgggcggac
      541 tcgggaccat gggcttcggg ctgccggcgg cgatcggtgc cgcgctcgcc gaaccgcagc
      601 gcccggtcgt gtgcttcagc ggcgacggct cgatcctgat gaacatccag gaactcgcga
      661 cgctcgccga ggaggacctc gacgtgaaga tcgtgctgtt caacaacggc gcgctcggcc
      721 tcgtgcacca gcagcagacg ctgttctacg gcggccggct gaacgcctcg gtgttccgcg
      781 gccagcccga tttcgtgcgc ctcgcccagg ccttcggcat ccgcaccatc gatctcgacg
      841 ccgaaccgga cgtgcccgat gcgctcgccg ccgccttcgc cgaacgcggt ccctgcctga
      901 tccacgcgac gatcgggctg gaggaaaagg tctacccgat ggtgccgccg ggcgccgcca
      961 acaccgacat gatcggaggc tgaaccatga atgccacccg taccgtgccc gccgcgctgg
     1021 ccgtgctcga actcaccgtc aacaaccacc ccggcgtcat gtcgcacgtc tgcggcctgt
     1081 tcgcccgccg cgcctgcaac gtcgaaggca tcatctgcct gccgctcggc gacggcgccc
     1141 gcagccgcat ctggctgcag gtgccggaca ccgacaagct cgaaccgatg acccgtcagg
     1201 cacgcaagct cgaagacgtg ctcgaagtca tccgccaccc ggacgggcat ccggtgtttg
     1261 cgcagctcgg ggcggcggtg ag
//
LOCUS       DAOQJH010000750         1282 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_111533_length_1282_cov_3.607987, whole genome shotgun
            sequence.
ACCESSION   DAOQJH010000750 DAOQJH010000000
VERSION     DAOQJH010000750.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1282
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111533_length_1282_cov_3.607987"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..545
                     /locus_tag="PLQ18_13905"
     CDS             <1..545
                     /locus_tag="PLQ18_13905"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acyltransferase"
                     /protein_id="HNE02797.1"
                     /translation="AGGRLIGCTRLLTDTAARAAGGFYSETEFELGPVRGLAGRIAEI
                     GRTCVHREHRNGATIATLWAGIAGFVAEHEVRYLIGCATVPLAEDMAQRVFSELAAQH
                     LTEDTLRVRPRQPLPRRDLDARGRAVLPPLLKAYLRVGARIAGEPCVDADFGCADLFI
                     LLDTVQLERRYARHFLDRPQ"
     gene            542..>1282
                     /locus_tag="PLQ18_13910"
     CDS             542..>1282
                     /locus_tag="PLQ18_13910"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNE02798.1"
                     /translation="MSAAGLMRVLRRAWRLPLLILLLGLGCAIAACLPGGAGRVPSPA
                     PMVWWSRRLCRLLGVRIVCHGTPLAGRALFVANHVSWLDIMVLAAVCPTHFLAKSEVA
                     GWPLFGWLCRRVGTAFIRRGAGDGAQAAAAELVWRLQRHDGRVLVFPEGTSTDGRGVR
                     RFHARLLQAAERAQCPVQALALRYPVAGGGTHPAVPFIGDDELLAHLWRLLGERRIEA
                     EVSCLAPLPAGIGRDALARRSHAQIAAVL"
BASE COUNT          131 a          464 c          487 g          200 t
ORIGIN      
        1 cggcgggcgg gcgactgatc ggctgcacgc ggctgctgac cgacaccgcg gcgcgcgccg
       61 ccggcgggtt ctactcggag acggaattcg agctgggacc ggtacgcggg ctcgccggac
      121 gcatcgccga gatcggccgc acctgcgtgc accgcgagca ccgcaacggc gcgacgatcg
      181 cgacgctgtg ggccggcatc gccggtttcg tcgccgaaca tgaggtccgg tatctgatcg
      241 gctgtgcgac cgtgccgctc gccgaggaca tggcgcaacg cgtgttctcg gagctggccg
      301 cgcagcacct gaccgaagac acgctgcgcg tgcgcccgcg ccagccgctg ccgcggcgtg
      361 atctcgatgc gcgcggcagg gccgtgctgc cgccgctgct gaaggcttat ctgcgcgtcg
      421 gtgcacgcat cgccggcgag ccctgcgtcg atgcggattt cggctgcgcc gatctgttca
      481 tcctgctcga caccgtgcag ctcgaacgcc gctacgcccg ccacttcctg gatcgtccgc
      541 agtgagcgcg gccgggctga tgcgtgtgct gcgccgggcc tggcggctgc cgctgctgat
      601 cctgctgctc gggctcggct gcgcgatcgc cgcctgcctg ccgggcggcg ccgggcgggt
      661 gccgtctccg gcgccgatgg tgtggtggag ccgccggctg tgccggctgc tcggcgtgcg
      721 catcgtctgt cacggcacgc cgctcgccgg ccgggcgctg ttcgtggcca accatgtgtc
      781 ctggctcgac atcatggtgc tcgccgcggt ctgtccgacg catttcctcg ccaagtccga
      841 ggtggccggc tggccgctgt tcggctggct gtgccggcgt gtcggcacgg ccttcatccg
      901 ccgcggggcc ggggacggcg cgcaggcggc ggctgcagaa ctggtctggc ggctgcagcg
      961 ccacgacggc cgcgtgctgg tattccccga gggcacctcg accgacgggc gcggcgtgcg
     1021 ccgcttccat gcgcggctgc tgcaggcggc cgagcgcgcg cagtgcccgg tgcaggcgct
     1081 ggcgctgcgc tatccggtcg ccgggggcgg cacgcatccg gccgtgccct tcatcggcga
     1141 cgacgaactg ctcgcgcatc tgtggcggct gctcggcgag cggcgcatcg aggccgaggt
     1201 gagctgcctc gcgccgctgc cggccggcat cggccgtgac gcgctggccc ggcgcagcca
     1261 cgcgcagatc gccgcggtgc tg
//
LOCUS       DAOQJH010000751         1282 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_111537_length_1282_cov_3.555827, whole genome shotgun
            sequence.
ACCESSION   DAOQJH010000751 DAOQJH010000000
VERSION     DAOQJH010000751.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1282
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111537_length_1282_cov_3.555827"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..318)
                     /locus_tag="PLQ18_13915"
     CDS             complement(<1..318)
                     /locus_tag="PLQ18_13915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNE02799.1"
                     /translation="MEPIWLAEYPPGVPATIDPDSTGTLPELFAESCARFADRAACRS
                     LGHEISYAELARRAGEFTAWLQGLGVAPGTRVALMMPNVLQYPVALFGTLAAGCTVVG
                     VNPL"
     gene            460..711
                     /locus_tag="PLQ18_13920"
     CDS             460..711
                     /locus_tag="PLQ18_13920"
                     /inference="COORDINATES: protein motif:HMM:NF024901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HNE02800.1"
                     /translation="MRALLLPLFAALLAAPAVTDAGEIYRWKDRDGAWHYGDRPPPGA
                     DAEWYDPRPRAGVDRQATPPAAGGSPGAPPAPKPSSGPR"
     gene            860..1120
                     /locus_tag="PLQ18_13925"
     CDS             860..1120
                     /locus_tag="PLQ18_13925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02801.1"
                     /translation="MTNLAAMLGRCVRRAICPVLAHDWRFRDIAADGRCTAYCRFCGK
                     CEPARLLLRSPPAAAAPPSVLAAAPTAPDVIDLQARQRELAA"
BASE COUNT          162 a          506 c          431 g          183 t
ORIGIN      
        1 cagcggattg acgccgacca ccgtgcagcc ggcggcgagc gtgccgaaca gcgccaccgg
       61 gtactgcagc acgttcggca tcatcagcgc gacgcgcgtg cccggcgcca cgccgaggcc
      121 ctgcagccag gcggtgaact cgccggcacg gcgcgcgagt tcggcgtagc tgatctcgtg
      181 cccgaggctg cgacaggccg cccggtcagc aaagcgtgcg caggattcgg cgaacagttc
      241 cggcagcgtg ccggtgctgt cggggtcaat cgtcgccggc acgccggggg ggtattcggc
      301 cagccagatc ggttccatgg ggggctcccg tgcggttgcg gaagaggatg gcgcctgcat
      361 cggtgttggc agccatcccg gcaagcgtat acggccgccc ccgcggcttc ctgccggcgc
      421 ggactagact cggagtcatc gccacgcccg gagaccgtca tgcgcgcgct cctcctgccg
      481 ctgttcgccg ccctgctcgc cgccccggct gtcaccgatg ccggcgagat ctaccgctgg
      541 aaggatcgcg acggtgcctg gcactacggc gaccgcccgc ctccgggcgc cgacgccgag
      601 tggtacgacc cgcgcccgcg tgccggcgtc gaccgtcagg cgacgccgcc ggcggccggc
      661 ggctcgccgg gcgcgccccc ggccccgaaa ccgtcctccg gcccgcgctg agaccgccag
      721 cccgtgtttt gccgcactgc gcaatccatc gctttgcgaa tttcttgcac ctgcacaatc
      781 gcgccgctag cctcgcaaga cctgatgctg caccagcaca aggacgcgcc ccctcccccc
      841 gttgcagagg cccgtcgcca tgacgaatct tgccgccatg ctcggccgct gcgtgcgtcg
      901 cgcgatctgc ccggtgctgg cccacgactg gcgtttccgc gacatcgcgg ccgacggacg
      961 ctgcaccgcg tactgccgct tctgcggcaa gtgcgaaccg gcacggctgc tgctgcgcag
     1021 cccgcccgcg gccgccgcac cgccttcggt gctggcggcg gccccgactg ctccggatgt
     1081 catcgacctc caggcccggc aacgggaact cgccgcatga gtccccggct gccgaccggc
     1141 ctgctgctcg gctgcgcctg ctgcctgctc gccggcctgc cggcgcttgc cgccggcgat
     1201 tccgtccgcc gggacgagct gttcggcctg atcccgaccg gtacgcgcac cggctcgaac
     1261 cccctgcagc gggccgacca gc
//
LOCUS       DAOQJH010000752         1282 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_111552_length_1282_cov_3.148329, whole genome shotgun
            sequence.
ACCESSION   DAOQJH010000752 DAOQJH010000000
VERSION     DAOQJH010000752.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1282
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111552_length_1282_cov_3.148329"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1282)
                     /locus_tag="PLQ18_13930"
     CDS             complement(<1..>1282)
                     /locus_tag="PLQ18_13930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02802.1"
                     /translation="AEVQALAVLQALAPELGTPAWQLALEAAGTTTEIRVRSLAVHRA
                     GSPLALTALGGISLAPDPAMSRIEFEGDWRALAWPIDGPAQIASPEGHFRVSGTQADW
                     RTTLKLLADVPEAGRLATEASVRGDTAHAVIESLAVRSPDGPAQIDASARVSFADLRF
                     AVDANWRQLGWPLKGKAQYASPKGRLQAEGTPKQYTYALELDAAGDSVPPLTLKASGD
                     GSDEAARLATLAITLLDGRIDGRAGLRWAPRLEWEAELAGQKLDPGRFAPEWPGRIAF
                     ELTSDGHIDADGQPQAHVLLSKLDGRLRDQPLDGRIDARVSGANLDLPAAKLVYGGTR
                     LDAEGRIHETWDLRWKLAAPDLARAAPGLRGRLSGDGQLSGPRERPQLQAKLDGQNLG
                     WPDGGLQTLAVRADVDSAGRRRSERGSAPEPPERV"
BASE COUNT          144 a          471 c          481 g          186 t
ORIGIN      
        1 gacccgttcg ggcggttcgg gcgcggaacc gcgttccgag cgccgccggc cggcggaatc
       61 gacatcggcg cgcacggcga gcgtctgcag gccgccatcc ggccagccga ggttctggcc
      121 gtcgagcttc gcctggagct gcgggcgttc gcgcgggccg ctgagctgac catcgccgct
      181 gagccggccg cgcaggcccg gcgcggctct ggcgagatcg ggcgcggcga gcttccagcg
      241 caggtcccag gtctcgtgaa tccggccttc ggcatcgagg cgcgtgccgc cgtagacgag
      301 cttcgccgcc ggcaggtcga ggttcgcgcc gctgacgcgc gcatcgatgc gcccgtcgag
      361 cggctgatcg cgcagccggc cgtcgagctt cgacagcagc acgtgcgcct gcggctggcc
      421 gtcggcatcg atgtggccgt cgctcgtgag ttcgaaggcg atgcgccccg gccattcggg
      481 cgcgaacctg ccgggatcga gcttctgccc ggcgagttcg gcctcccatt cgagccgtgg
      541 cgcccagcgc agcccggccc tgccgtcgat gcggccatcg agcagcgtga tcgcgagcgt
      601 tgccagcctg gccgcctcgt cgctgccgtc gccgctcgcc ttcagcgtca gcggcggcac
      661 gctgtcgccg gcggcgtcga gttcgagcgc atacgtgtac tgcttcggcg tgccctcggc
      721 ctggagcctg cccttcgggc tcgcgtactg cgccttgccc ttcagcggcc agccgagctg
      781 gcgccagttc gcgtccaccg cgaagcgcag gtcggcgaag ctcacgcgag cgctggcgtc
      841 gatctgcgcc gggccgtccg gcgagcgcac ggccagcgat tcgatgacgg cgtgcgcggt
      901 gtcgccgcgc acgcttgcct cggtcgcgag ccgcccggcc tcgggcacgt cggcgagcag
      961 cttcagcgtc gtgcgccagt cggcctgcgt gccggaaacc cggaagtgac cctcgggact
     1021 cgcgatctgc gccgggccgt cgatcggcca ggcgagggca cgccagtcgc cttcgaactc
     1081 gatgcggctc atcgccgggt cgggcgcgag cgagatgccg ccgagcgcgg tcagcgccag
     1141 cgggctgccg gcgcgatgca cggcgagcga gcgcacgcgg atttcggtcg tggtgccggc
     1201 ggcttcgagt gcgagctgcc aggccggcgt gccgagctcg ggcgcgagcg cctgcagcac
     1261 cgctagcgcc tgcacctcgg cc
//
LOCUS       DAOQJH010000753         1280 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_111870_length_1280_cov_4.382041, whole genome shotgun
            sequence.
ACCESSION   DAOQJH010000753 DAOQJH010000000
VERSION     DAOQJH010000753.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1280
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111870_length_1280_cov_4.382041"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..739)
                     /gene="hisS"
                     /locus_tag="PLQ18_13935"
     CDS             complement(<1..739)
                     /gene="hisS"
                     /locus_tag="PLQ18_13935"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007111598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HNE02803.1"
                     /translation="MASAKNLQAIRGMNDLLPADAARWAGFEALIRDVLTGYGYREIR
                     TPIVEATELFCRSIGEVTDIVEKEMYTFADRNGDSLTLRPEGTASCVRACIEHGLLHN
                     QQQRLWYAGPMFRHERPQKGRYRQFHQIGAEAFGMDGPDIDAEMICMTARFWQRLGLA
                     DVRLQLNSLGTPACRSVYRDKLVAHLRSGYELLDEDARRRLDSNPLRILDTKNPAMRE
                     LVDAAPVLADHLDDESARHFERLRALLD"
     gene            complement(809..>1280)
                     /locus_tag="PLQ18_13940"
     CDS             complement(809..>1280)
                     /locus_tag="PLQ18_13940"
                     /inference="COORDINATES: protein motif:HMM:NF024855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4115 domain-containing protein"
                     /protein_id="HNE02804.1"
                     /translation="APAAAPAAVNPVAATPGHTAAPAPSMNPVPPATASPALAAAAAT
                     PPLPVANVPPPAAGAAPAPAPAGPAAPAESRIELQASADSWAEVRDAGGKLLFQGTLR
                     PGSPQSITGRPPFRMKLGHAQHVTIRLDDSLVDSGVYVPKRGSVARFTLGREPG"
BASE COUNT          176 a          431 c          480 g          193 t
ORIGIN      
        1 catcgagcag cgcgcgcagg cgctcgaagt gccgcgccga ttcgtcgtcg aggtgatcgg
       61 cgagcaccgg cgcggcgtcg acgagttcgc gcatcgccgg gttcttggta tcgaggatgc
      121 gcagcggatt gctgtcgagc cgccggcgcg cgtcctcgtc gagcagctcg taaccgctcc
      181 tcaggtgcgc gaccagcttg tcgcgataga cgctgcgaca ggccggcgtg ccgagcgagt
      241 tcagctgcag gcgcacgtcg gcgagtccga gccgctgcca gaagcgcgca gtcatgcaga
      301 tcatctcggc atcgatgtcc gggccatcca tgccgaaggc ttcggcgccg atctggtgga
      361 actgccggta gcggcccttc tgcgggcgct cgtggcggaa catcggcccg gcgtaccaga
      421 gccgctgctg ctggttgtgc agcaggccgt gctcgatgca ggcgcgcacg cagctcgccg
      481 tgccctcggg gcgcagcgtc aggctgtcac cgttgcggtc ggcgaaggtg tacatctcct
      541 tctcgacgat gtcggtgacc tcgccgatcg agcggcagaa gagttccgtg gcctcgacga
      601 tcggcgtgcg gatctcgcga tagccgtagc cggtgagcac gtcgcggatc agcgcctcga
      661 agccggccca gcgggcggca tcggccggca gcaggtcgtt catgccgcgg atcgcttgca
      721 ggttcttcgc gctcgccatc gtgttcgctg tatgtgggga aggtcgggaa gggaaggtgt
      781 cgggaggaat gccgggcagc cggccgcttc agcccggttc gcggccgagc gtgaagcgcg
      841 cgacggaacc gcgcttcggc acgtagaccc cggagtcgac gaggctgtcg tcaagccgga
      901 tcgtgacgtg ctgcgcgtga ccgagcttca tgcgaaacgg cggccgacca gtgatcgact
      961 gcgggctgcc cggacgcagc gtgccctgga acagcagctt gccgccggca tcgcgcacct
     1021 cggcccagct gtcggcactg gcctgcagct cgatccgcga ctcggccgga gccgccgggc
     1081 cagccggcgc tggcgccggc gcagcacccg cagccggtgg cggtacgttc gcgaccggca
     1141 ggggcggcgt cgccgccgcc gccgccaggg ccggcgaggc cgtggccggg ggcaccggat
     1201 tcatcgacgg cgccggcgca gccgtatgcc cgggcgtggc ggccaccgga ttcactgctg
     1261 ccggtgcggc agccggtgcc
//
LOCUS       DAOQJH010000754         1274 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_112964_length_1274_cov_4.260870, whole genome shotgun
            sequence.
ACCESSION   DAOQJH010000754 DAOQJH010000000
VERSION     DAOQJH010000754.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1274)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1274)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1274
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112964_length_1274_cov_4.260870"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="PLQ18_13945"
     CDS             <1..261
                     /locus_tag="PLQ18_13945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008622301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HNE02805.1"
                     /translation="GGSGIGLIEAARGSLGHWLGIDGGRIRHYQIIAPTTWNFSPRSA
                     DGVPGPLEQALIGTAVEPGETVPLAVQHVVRSFDPCMSCTVH"
     gene            379..777
                     /locus_tag="PLQ18_13950"
     CDS             379..777
                     /locus_tag="PLQ18_13950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02806.1"
                     /translation="MDAWMRLVRQHQGGFTSSAAAADGGAVTIAVDVFELPVMRQNRA
                     DQALEAGMKPLEEVCHAVSPLHPGPHVHGRNRSSADEARSPFGKQQVLEILIDINFAS
                     LSAHSALSIRQAPNPQGISIGDADIPAIFH"
     gene            854..>1274
                     /locus_tag="PLQ18_13955"
     CDS             854..>1274
                     /locus_tag="PLQ18_13955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HNE02807.1"
                     /translation="MSLTRRRFLKYAAASASAAALARLPTASAATAAVPVAAIALDGF
                     TTFDPRPLAALAEQMFPDGAGKALTLAWRTRQFEYTWLRTLMNRYADFWQVTGEALDF
                     AAAQTGLKLGGEQRDRLMQGWLELRAWPLNSGHLEPSE"
BASE COUNT          214 a          418 c          419 g          223 t
ORIGIN      
        1 gggggttcgg gcatcggact gatcgaggcg gcgcgcggca gtctcggcca ctggctcggc
       61 atcgacggcg ggcgcatccg ccactaccag atcatcgcgc cgaccacgtg gaacttctcg
      121 ccgcgctcgg ccgacggcgt gccgggaccg ctcgaacagg ccttgatcgg cacagcggtc
      181 gagcccggcg aaaccgtgcc gcttgcggtg cagcacgtcg tgcgctcgtt cgatccgtgc
      241 atgagctgca cggtccactg atacggccac tgacacgaac tgctgacacg acgacgcccg
      301 gtggcttgca cggccatccg gacgtcagtg gacaggcgct gtcaggtttc gggcagggcg
      361 gggagcgagg gatcaggcgt ggacgcctgg atgaggctcg tgcgtcagca ccagggcggg
      421 ttcacgtcgt ccgcagcggc ggcagacggc ggtgcagtga ccatcgcggt ggatgttttc
      481 gaattgccag tcatgcgcca gaaccgggca gatcaggcgc ttgaagccgg catgaagccg
      541 cttgaggaag tttgccatgc tgtgtctccg ctacatcctg gaccgcacgt ccatgggcgg
      601 aatcggtcaa gcgccgatga agctagaagc ccgttcggga agcagcaagt gcttgaaatc
      661 cttattgaca ttaactttgc gtcactttct gcccattctg ctttgtccat acggcaagca
      721 ccgaatccgc aaggcatttc catcggcgac gcggatattc cggcaatttt ccattgacgt
      781 ttcgttacat ccggcatccg ggctccgcgt ggctgcgtag ccagcgacac ccctcatcca
      841 gccggaatcc gccatgtccc tcacccgtcg ccgcttcctg aagtacgccg ccgccagcgc
      901 cagcgcagcg gcactcgccc gcctgccgac ggcttctgcc gccacggccg cggtgccggt
      961 cgctgccatc gccctcgacg gcttcacgac cttcgatcca cgcccgctgg ccgccctggc
     1021 cgagcagatg tttccggatg gagcgggcaa ggccctgacg cttgcgtggc gcacgcgcca
     1081 gttcgaatac acctggctgc gcacgctgat gaaccgctac gcggatttct ggcaggtcac
     1141 cggggaagcg ctggacttcg ctgcagcgca gacgggcctg aagctcggcg gcgaacagcg
     1201 ggatcggctg atgcagggct ggctggagct gcgcgcctgg ccattgaaca gtggccatct
     1261 ggagccgagc gagg
//
LOCUS       DAOQJH010000755         1270 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_113687_length_1270_cov_3.652675, whole genome shotgun
            sequence.
ACCESSION   DAOQJH010000755 DAOQJH010000000
VERSION     DAOQJH010000755.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1270
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113687_length_1270_cov_3.652675"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..328)
                     /locus_tag="PLQ18_13960"
     CDS             complement(<1..328)
                     /locus_tag="PLQ18_13960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02808.1"
                     /translation="MSSIGAMPTAHSVAVALGSALTSGPAPVPASAVPGCGSVAASLR
                     AGREAGGVGGAAGADVGVGGGASVLAGAASAAADGPRAADTAASKSASAVSTAARRSV
                     SPGRARQ"
     gene            318..>1270
                     /locus_tag="PLQ18_13965"
     CDS             318..>1270
                     /locus_tag="PLQ18_13965"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE02809.1"
                     /translation="MLLTTLSPLELQLRTFADLDAALSALEAGVTAPAATLIGTAMLG
                     RPEAIARLRARAPLALYSGRGSLADRIAGVHAGAETCFAATVDQALLGEWLEMRCVPR
                     VGDALRVLLIDDDPIFIALCSNLLSGNGFVVRAMEHPYALCEELAEFEPDVLLLDLYL
                     RDCTGLEVATALRQFPGWSNLPIVFLSADHELAHQAAALGAGLDDWLSKPFEAVQLRQ
                     VVQTRALRGRRLRARVDTDPLTGVLSASSFENRLGIEVGRAQRYGRALSLVLIDPDEL
                     GALADAQGRGAADRLLCLLAQQLGQRVRRSDLLGRGHGAAFA"
BASE COUNT          150 a          489 c          454 g          177 t
ORIGIN      
        1 cctggcgtgc ccggcccggc gagaccgatc tgcgcgccgc ggtcgacacg gcgctggcgg
       61 acttcgaggc cgcggtgtcg gcagccctcg ggccgtctgc agcggcggat gccgcgccgg
      121 cgagcaccga cgcgccgccg ccaacgccga catccgcgcc cgccgcgcct ccgacgcctc
      181 cggcctcccg gccggcgcgc agcgaagccg ccaccgagcc gcagcccggc acagctgagg
      241 ccgggaccgg ggccgggccc gaagtcaaag ccgagcccag tgccacggcc acgctgtggg
      301 ctgtcggcat cgcaccgatg ctgctcacga ccctgtcgcc gctcgaactg cagttgcgga
      361 ccttcgccga cctcgatgcc gcgctgtcgg cgctggaggc cggcgtcacg gccccggccg
      421 cgacgctgat cggcaccgcg atgctcgggc ggcccgaggc catcgcccgg ctgcgcgcac
      481 gcgcaccgct tgcgctgtac agcggtcgcg gcagcctcgc cgaccgcatc gccggcgtgc
      541 atgccggggc cgagacctgt ttcgccgcga ccgtcgatca ggccctgctc ggtgaatggc
      601 tggagatgcg ctgcgtgccg cgcgtcggcg atgcgctgcg cgtgctgctc atcgacgacg
      661 acccgatatt catcgcactg tgcagcaacc tgctgtccgg caacggtttc gtggtgcggg
      721 cgatggaaca cccgtacgcg ctctgcgagg agctggccga gttcgagccg gatgtgctgc
      781 tgctcgatct gtacctgcgc gactgcaccg gcctggaggt cgccaccgcc ctgcgccagt
      841 tcccgggctg gtcgaacctg ccgatcgtgt tcctgtcggc cgatcacgag ctggcgcatc
      901 aggccgctgc cctcggggcc gggctcgatg actggctgtc gaagccgttc gaggccgtgc
      961 agctgcgtca ggtcgtgcag acgcgcgcgc tgcgcggccg ccgcctgcgc gcccgggtcg
     1021 acaccgatcc gctgaccggc gtgctgtcgg caagcagctt cgagaaccgc ctcggcatcg
     1081 aggtcggccg tgcccagcgc tacggccggg ccctgtcgct ggtgctgatc gatccggacg
     1141 aactcggcgc gctcgccgat gcccagggcc gcggcgccgc cgaccgcctg ctgtgcctgc
     1201 tcgcgcagca gctcgggcag cgcgtgcgcc gcagcgacct gctcggacgc ggccacgggg
     1261 ccgccttcgc
//
LOCUS       DAOQJH010000756         1260 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_115489_length_1260_cov_3.300415, whole genome shotgun
            sequence.
ACCESSION   DAOQJH010000756 DAOQJH010000000
VERSION     DAOQJH010000756.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1260)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1260)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1260
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115489_length_1260_cov_3.300415"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(370..1182)
                     /locus_tag="PLQ18_13970"
     CDS             complement(370..1182)
                     /locus_tag="PLQ18_13970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011491828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNE02810.1"
                     /translation="MSDRHYTRLSPAEAETLLATGLASVFDVREKPFFAQDHLPGARH
                     LDDAGMDAALLGTPRRQPVLIYCHHGNASRAVAQAFADFGFTQVSDLAGGWEAWRAHR
                     AAGIQAVRGVAPREPLPAVLADWLQAEGYPADDLDARLANGTTPLMRAARLGLADRVA
                     DLVIAGAPLESRNADGNTALWLACFSDDADTLDILLNAGASPDNLNDNGATCLMYAAS
                     AGKAGVVQALLAAGADASLRSLDDFTALDMASTEACLNLLRPGRGRRAPGTA"
BASE COUNT          192 a          400 c          465 g          203 t
ORIGIN      
        1 ctagctggct tcctcgccat gcgcggcggg aagttgatgg ctgctgacgg taaacgtcgg
       61 aagctgggcc cgcttcttga gccgggcgct gacgcattct gcaaagcaag ttgcgagcca
      121 tggcgtggcg acatctttcg acaatggtcg aagcgatggc gcttctctcc cctacgtttc
      181 ccgccacgat ccggttacag gaaaacgctc cggatcaggg ccttgaggtg ttgaagcgga
      241 ttgaacgtcc tgacagcgac tggccggtgg cccggaactc gtcgcaggct tgtcgtgcgc
      301 aggccggtgg gcgagtgtca ggatttttaa cgctgcattg caccatcgcg gaccgcaaag
      361 atgcccgcct caggccgtgc ccggggctct ccgtccgcgt ccggggcgca gcaggttcag
      421 gcaggcttcg gtcgaggcca tgtccagcgc cgtgaagtca tcgagcgagc gcaggctggc
      481 gtcggccccg gcggcgagca gcgcctgcac gaccccggcc ttgccggccg aggctgcgta
      541 catcaggcag gtcgcgccgt tgtcgttgag gttgtcgggg ctggcgccgg cgttcagcag
      601 gatgtcgagc gtgtcggcat cgtcgctgaa gcaggcgagc cacagcgcgg tgttgccgtc
      661 ggcattgcgc gattcgagcg gtgcaccggc gatcaccaga tcggcgaccc gatcggccag
      721 tccgaggcgc gcggcgcgca tcagcggtgt cgtgccgttg gcaagccgcg catcgagatc
      781 atccgccgga tagccttcgg cctgcagcca gtcggcgagc accgccggca gcggttcgcg
      841 cggggcaacg ccgcgcaccg cctggatgcc cgccgcccgg tgcgcacgcc aggcttccca
      901 gccgccggcg agatcgctga cctgcgtgaa gccgaagtcc gcgaaggcct gcgcgaccgc
      961 gcggctggcg tttccatgat ggcagtagat cagcaccggc tggcgacgcg gcgtgccgag
     1021 cagcgcggca tccatgccgg catcgtcgag gtgtctggcg ccgggcagat gatcctgcgc
     1081 aaagaacggc ttctcgcgca cgtcgaagac gctggccagg ccggtggcga gcagggtttc
     1141 ggcttcggcc gggctcaggc gggtgtaatg gcgatcgctc atcggcagca ggctccgggg
     1201 caggcggaaa ggggcgggac aggggaatca gcggcggtcg ggatacggcg gctcgctggc
//
LOCUS       DAOQJH010000757         1260 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_115523_length_1260_cov_2.764315, whole genome shotgun
            sequence.
ACCESSION   DAOQJH010000757 DAOQJH010000000
VERSION     DAOQJH010000757.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1260)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1260)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1260
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115523_length_1260_cov_2.764315"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..140)
                     /locus_tag="PLQ18_13975"
     CDS             complement(<1..140)
                     /locus_tag="PLQ18_13975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoadipate CoA-transferase"
                     /protein_id="HNE02811.1"
                     /translation="MNRMTRDQLAARVARDIPEGAYVNLGIGAPTRVANYLPKDREIF
                     LH"
     gene            complement(163..846)
                     /locus_tag="PLQ18_13980"
     CDS             complement(163..846)
                     /locus_tag="PLQ18_13980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014087563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit A"
                     /protein_id="HNE02812.1"
                     /translation="MIDKTCTSLAAAVAGIHDGATVMIGGFGTAGMPSALIDALIDQG
                     AKDLTIVNNNAGNGDTGLAALLKARRVRRIICSFPRQADSYVFDALYRAGEIELEVVP
                     QGNLACRIEAAGAGLGAIYAPTGYGTLLAEGKETRRIDGRDYVLEWPIRADFALIKAQ
                     HGDRWGNLVYRKSARNFGPIMARAARCTIAQVEAVLELGSLDPECIVTPGIFVQRVVP
                     VRDAVQAAA"
BASE COUNT          174 a          411 c          463 g          212 t
ORIGIN      
        1 ctgtgcagga agatctcgcg atccttcggc aggtagttgg cgacgcgcgt cggcgcaccg
       61 atgccgaggt tcacgtaggc gccttcgggg atgtcgcgcg ccacgcgggc ggcgagctgg
      121 tcgcgggtca tacggttcat ggcgatggtt cctcgcgcgg cgtcaggcgg cggcctgcac
      181 ggcgtcgcgc accggcacga cgcgctggac gaagatgccg ggggtgacga tgcactccgg
      241 atcgagactg ccgagctcaa gcaccgcctc gacctgcgcg atcgtgcagc gcgcggcgcg
      301 cgccatgatc gggccgaagt tgcgcgcgct cttgcggtag acgaggttgc cccagcgatc
      361 accgtgctgc gccttgatca gcgcgaagtc ggcgcggatc ggccattcga gcacgtagtc
      421 gcgcccgtcg atgcggcgcg tctccttgcc ctcggcgagc agcgtgccgt agccggtcgg
      481 cgcgtagatc gccccgagcc cggcgccggc agcctcgatg cggcaggcga gattgccctg
      541 cggcacgact tccagctcga tctcgccggc gcggtacagc gcgtcgaaca cgtaggaatc
      601 cgcctggcgc gggaacgagc agatgatccg gcgcacgcgg cgcgccttca gcagcgccgc
      661 cagaccggtg tcgccgttgc cggcgttgtt gttgacgatc gtcaggtctt tcgcgccctg
      721 gtcgatcagc gcatcgatca gcgccgacgg catgccggcc gtgccgaagc cgccgatcat
      781 cacggtcgcg ccgtcatgga tgccggcgac ggcggcggcg agcgaagtgc aggtcttgtc
      841 gatcatgccg gctgcctccg tgcggggcgg cggacgggga cgcgcggcgc ggcgtcctgc
      901 gggcggtgcg gctggttcat gttgcctccg gtggtttgtt cgtttatcga acttatgtgc
      961 gatgtgtgaa cgaaacacta aagtccgccg ctgactgcgt caattgcgtt tatcgaaatg
     1021 ttgttcgttt atcgaacgat aatgaccatc gtgtccggac tccggcggcc cgtctgccgg
     1081 gcaggccgcg cgccgggcca cgttccgggt tcggcggcgg atgtcgcgcc cggctgccgg
     1141 ccggaaacga caccgcccgc cgtcggctga gccggcggcg ggcggtcacg gggatggctg
     1201 ctgcggacgg atcagctgac gagctgcatg ccgagttcgg cgctcgcgcc gagcaggacc
//
LOCUS       DAOQJH010000758         1254 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_116572_length_1254_cov_4.983319, whole genome shotgun
            sequence.
ACCESSION   DAOQJH010000758 DAOQJH010000000
VERSION     DAOQJH010000758.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1254
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116572_length_1254_cov_4.983319"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..720)
                     /locus_tag="PLQ18_13985"
     CDS             complement(<1..720)
                     /locus_tag="PLQ18_13985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007778106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNE02813.1"
                     /translation="MTTAYDAAAIEVLSGLDPVRKRPGMYTDTARPNHLAQEVIDNSV
                     DEAMAGHAHAIDVVLHADGSVTVTDDGRGMPVDLHPEEGIPGVELILTRLHAGGKFSN
                     ANYRFSGGLHGVGVSVVNALSKRLEVVVKRGGQRWGMQFADGNKASELAALGSCPKRE
                     TGTTVRFWPDPRYFDSPKLSVSRLRHVLRAKAVLCPGLRVSFTDEEANERVEWCYSDG
                     LTDYLKEALAGVELLPDPPFTG"
     gene            886..>1254
                     /locus_tag="PLQ18_13990"
     CDS             886..>1254
                     /locus_tag="PLQ18_13990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HNE02814.1"
                     /translation="MTRLLARLLRRSIPPALSHGNALDIHTDGDACFEALWADLDAAG
                     QSIWMETYILDPDAIGLETLARLRAAAARGCEVILVYDAVGSGSLGERHLEALHAAGA
                     RVHAFNPLLRNPLRRAHAHDH"
BASE COUNT          186 a          435 c          418 g          215 t
ORIGIN      
        1 gccggtgaac ggcggatcgg gcagcagctc gacgccggcg agcgcttcct tcaggtaatc
       61 ggtgaggccg tcgctgtagc accactcgac gcgctcgttc gcttcctcgt cggtgaagct
      121 cacgcgcagg cccggacaca gcacggcctt ggcgcgcagc acgtgccgca gccgcgacac
      181 cgacagcttc ggggagtcga agtagcgcgg gtcgggccag aagcgcaccg tcgtgccggt
      241 ctcgcgcttc gggcagctgc cgagcgcggc gagctcgctc gccttgttgc cgtcggcgaa
      301 ctgcatgccc cagcgctggc cgccgcgctt cacgacgact tcgagccgct tcgacagcgc
      361 gttgacgacc gacacgccga cgccgtgcag accgcccgag aagcggtagt tggcgttcga
      421 gaactttccg ccggcgtgca gccgcgtcag gatcagctcg acgccgggaa tgccttcctc
      481 gggatgaagg tccaccggca tgccgcggcc gtcgtcggtg accgtcaccg agccatcggc
      541 gtgcagcacg acatcgatgg cgtgcgcgtg gccggccatc gcttcgtcga cgctgttgtc
      601 gatgacttcc tgcgcgagat gattcggacg tgcggtgtcg gtgtacatgc ccgggcgctt
      661 gcgcaccggg tccagcccgc tcagcacttc gatcgcggcg gcgtcgtagg cggtggtcat
      721 gggtctcgtg aagtcgtcgg aaaaacgcac gtgcgttgtt gcgctgcagc tgttacgtgc
      781 agttgcagca tcagtgtaac cgacctgctg ccgggatgac gcacgatccc ttgcattacg
      841 ttcatgtttt cctgtccgac cagagccatc gaggatccgc gaacgatgac ccgattgctc
      901 gcccgcctgc tgcgccgctc gattccgccg gccctgagcc atggcaacgc gctcgacatc
      961 cacaccgacg gagatgcctg cttcgaggcg ctgtgggcgg atctggacgc cgccgggcag
     1021 tcgatctgga tggaaaccta catcctcgat cccgatgcca tcggtctcga aaccctggcc
     1081 cgcctgcggg cggcggccgc gcgcggctgc gaggtgatcc tggtctacga cgcggtcggt
     1141 tcgggcagcc tcggcgagcg ccacctcgaa gccctgcacg cggccggtgc ccgggtgcat
     1201 gcgttcaacc cgctgctgcg caatccgctg cgccgcgccc atgcccacga ccac
//
LOCUS       DAOQJH010000759         1247 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_117922_length_1247_cov_4.588926, whole genome shotgun
            sequence.
ACCESSION   DAOQJH010000759 DAOQJH010000000
VERSION     DAOQJH010000759.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1247
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117922_length_1247_cov_4.588926"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..550)
                     /locus_tag="PLQ18_13995"
     CDS             complement(<1..550)
                     /locus_tag="PLQ18_13995"
                     /inference="COORDINATES: protein motif:HMM:NF023168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HNE02815.1"
                     /translation="MARTTDTRALTEQAFWELLATARKPTIQAVQEWLLARGLTRRNN
                     NTIGSALEECWQQLGTRLKQERSAPELPEDVVDQVLNLRRRMLEVASSVFDEDRAKLR
                     EEVESIRQQAASSIAEAERGQRELAERLQGSELSAGEARAALAEREARLADLLAELAA
                     VRSDAAAATERAAATAAQVIALE"
     gene            complement(686..1093)
                     /locus_tag="PLQ18_14000"
     CDS             complement(686..1093)
                     /locus_tag="PLQ18_14000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02816.1"
                     /translation="MQRDTVVARYAIALVARERQRQLAVEGWSEAHDDALTGGELGLA
                     AACYAAAPQPLYTGSPEAPVDAWPFAPAQDKRGRHDTVRRLAIAGALLLAELERELRR
                     GGLAEAAAADPDLVNELYAAARAVSRQGRLQDE"
BASE COUNT          153 a          437 c          460 g          197 t
ORIGIN      
        1 gttccagggc aatgacctgc gccgccgtcg cggcggcgcg ttcggtggcg gcggcggcat
       61 cgctgcgcac cgccgcgagt tcggcgagca gatcggcgag tcgcgcctcg cgctcggcga
      121 gggcggcccg ggcttcgccg gcgctcagct cgctgccctg cagccgctcg gcgagttcac
      181 gctggccgcg ctcggcctcg gcgatgctgc ttgcggcctg ctgccggatc gactcgactt
      241 cctcgcgcag tttcgcgcgg tcctcgtcga acaccgagga ggcaacctcc agcatgcgcc
      301 ggcgcaggtt cagcacctga tcgacgacat cctcgggcag ttccggggcg ctgcgctcct
      361 gcttcaggcg cgtgccgagc tgctgccagc attcctccag cgccgagccg atcgtgttgt
      421 tgttgcgccg ggtcaggcca cgggcgagca gccattcctg caccgcctga atggtgggct
      481 tgcgcgccgt cgcgagcagt tcccagaacg cctgttcggt cagggcacgg gtatcggtcg
      541 tgcgggccat ctctgcatac tcgccgggga gccgcggcga attttatgca cagttgacgt
      601 gattaacgtg atttttaact tggtccgccg gcgaaagcga cacgcattgc gaccggaacg
      661 acaccgaagc ggccgcaagc cccgttcatt cgtcctgcag ccggccctga cggctgaccg
      721 cacgtgccgc cgcgtacagt tcgttgacca gatcgggatc ggccgccgcc gcctcggcga
      781 ggccgccgcg gcgcagttcg cgttcgagtt cggcgagcag cagcgcaccg gcgatcgcga
      841 gccggcgcac cgtgtcgtgc cggccgcgct tgtcctgcgc gggcgcgaac ggccaggcgt
      901 ccacgggggc ttcgggactg ccggtgtaca gcggctgcgg cgcggcggcg tagcaggcgg
      961 cggcgaggcc gagttcgccg ccggtcagcg cgtcgtcgtg ggcctcgctc cagccctcga
     1021 cggcgagctg gcgctggcgc tcgcgggcga ccagcgcgat cgcgtagcgg gcgacgacgg
     1081 tgtcacgctg catgacggga agctccggcg ccggctgcgc tgaggtgcgg ttccgggcgg
     1141 gcttcagtcg cgccgcgccg gatacagcgg ggccagcgac ggtgccggcg tggcttcgcc
     1201 ggtctgcatc gcctgcggca ggcgctgcag cgagccgagc cactgca
//
LOCUS       DAOQJH010000760         1243 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_118692_length_1243_cov_4.082492, whole genome shotgun
            sequence.
ACCESSION   DAOQJH010000760 DAOQJH010000000
VERSION     DAOQJH010000760.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1243
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118692_length_1243_cov_4.082492"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            3..917
                     /locus_tag="PLQ18_14005"
     CDS             3..917
                     /locus_tag="PLQ18_14005"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018509441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HNE02817.1"
                     /translation="MLADSARELLRERRRERRWRLFFRATWLIVVLLVLAQWRAGSDM
                     TAATLGGGRHAAKVSVDGLIAPGTDVSAERVLHGLRTAFADREAAGIILDINSPGGSP
                     VQAGQVYAELRRLRAQHPDKPVYAVIGDVGASGAYYIAAGAQEIYADRASLVGSIGVR
                     MDGFGFVEAMQRLGIERRLLTSGANKGLLDPFSPVQPAQLAHVQTLLDAIHQQFIAAV
                     REGRGERLHETPEIFSGLFWTGEQAQALGLVDGYGSVESIARDKLGTTQIRDHTQQEK
                     PFERLFGQVESTLAGALTRAFGLDSPLR"
     gene            1030..>1243
                     /locus_tag="PLQ18_14010"
     CDS             1030..>1243
                     /locus_tag="PLQ18_14010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2760 domain-containing protein"
                     /protein_id="HNE02818.1"
                     /translation="MSEPTPSIFERVFLAFSAFGRTLASGRFAAGVARLRHGDAGASA
                     AAALPVVMPEPLAAVPPPPPAPVAPPV"
BASE COUNT          162 a          469 c          435 g          177 t
ORIGIN      
        1 cagtgctcgc cgacagcgcg cgcgagctgc tgcgcgaacg ccggcgggag cggcgctggc
       61 ggctgttctt ccgtgccacc tggctgatcg tggtgctgct cgtgctcgcg cagtggcgcg
      121 ccggcagcga catgacggcg gccacgctcg gcggcggccg gcacgcggcg aaggtcagcg
      181 tcgacggcct gatcgccccg ggcaccgacg tcagcgccga gcgcgtgctg cacggcctgc
      241 gcacggcctt cgccgatcgc gaggcggccg gcatcatcct cgacatcaac agccccggcg
      301 gcagcccggt gcaggccgga caggtctacg ccgaactgcg ccggctgcgt gcgcagcatc
      361 cggacaagcc cgtgtacgcg gtgatcggcg atgtcggcgc ctccggtgcg tactacatcg
      421 ccgccggcgc gcaggagatc tacgccgatc gcgcgagtct ggtcggctcg atcggcgtgc
      481 gcatggacgg cttcggtttc gtcgaggcca tgcagcgcct cggcatcgaa cggcggctgc
      541 tgacctcggg cgccaacaag ggcctgctcg atccgttcag cccggtgcag ccggcgcagc
      601 tcgcgcacgt gcagacgctg ctcgatgcca tccaccagca gttcatcgcc gcggtgcgcg
      661 aaggccgcgg cgagcgcctg cacgagacgc cggagatctt cagcggcctg ttctggaccg
      721 gcgagcaggc gcaggcgctc gggctggtcg atggctacgg cagcgtcgaa tccatcgccc
      781 gcgacaagct cggcaccacg cagatccgcg accacacgca gcaggaaaaa cccttcgagc
      841 ggctgttcgg ccaggtcgaa tcgacgctgg caggcgcact gacccgcgcc ttcgggctcg
      901 acagtccgct gcgctgattc cggccgccat gcaccgcctt ccgcaccgcg cgcaggccgc
      961 tgcgcgcggc ttccgtccct gtcccccctc tgaaccggaa tcgaaccgga cctgcaggag
     1021 cgcccgcccg tgagcgaacc gacgccctcg atcttcgaac gtgtcttcct ggccttcagt
     1081 gccttcggcc gtacgctggc ctcgggccgc tttgccgccg gcgtggcccg gctgcgccac
     1141 ggcgatgccg gtgcgagcgc agccgccgcg ctgccggtgg tgatgccgga gccgctcgct
     1201 gccgtgcccc cgccgcctcc ggccccggtg gcgccgccgg tgg
//
LOCUS       DAOQJH010000761         1218 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_123792_length_1218_cov_4.097163, whole genome shotgun
            sequence.
ACCESSION   DAOQJH010000761 DAOQJH010000000
VERSION     DAOQJH010000761.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1218)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1218)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1218
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123792_length_1218_cov_4.097163"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..322
                     /gene="sugE"
                     /locus_tag="PLQ18_14015"
     CDS             2..322
                     /gene="sugE"
                     /locus_tag="PLQ18_14015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HNE02819.1"
                     /translation="MNWLILLVAGLCEVGWAIGLKYTEGYTRLWPSVFTIAGMVVSVW
                     LLGIAMRTLPVGTAYAVWVGIGAVGTAILGIVLFDEPADAARLFSLTLIVAGIVGLKL
                     ASAH"
     gene            479..>1218
                     /locus_tag="PLQ18_14020"
     CDS             479..>1218
                     /locus_tag="PLQ18_14020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02820.1"
                     /translation="MTLSTDPLAAWQALPEPARVFADALAVLYEPLAFAHWPELYAGL
                     GFAKKGAAAQKAVQSLPDTLRPLLRPKAGRDPGPPVLDAAVSERLMRRLGREPEALRR
                     FADQVLRAVPAASLGAALAGRVDPAVFQQLLATSLIVNHSRRDLRLAVHANDFDTALR
                     IAREMQNGDPDRWFMERPLTQLVRCAYDADWLFQREPRLRWMMIEDWLGTLVGDLEDP
                     REVRALLDRCRNAPQQQALFFDRLLAQEL"
BASE COUNT          157 a          432 c          427 g          202 t
ORIGIN      
        1 gatgaactgg ctgatcctgc tggttgccgg tctctgcgag gtcggctggg cgatcgggct
       61 gaagtacacc gagggctaca cccggctgtg gccgagcgtg ttcacgatcg ccggcatggt
      121 cgtcagcgtc tggctgctcg gcatcgccat gcgcacgctg ccggtcggca ccgcctacgc
      181 ggtctgggtc ggcatcggcg cggtcggcac cgcgatcctc ggcatcgtgc tgttcgacga
      241 accggccgat gccgcgcgcc tgttcagcct gacgctgatc gtcgccggca tcgtcgggct
      301 gaagctcgcc agcgcgcact gagcgcaccg gcccggtgga agcagcgcgg cttgcgcgtg
      361 gccggcgctt ccggcgcccg ccctgagcgg attcggaacc ttcgccgccc cgggcccggc
      421 gatgaatctg cctgtccggg agcctgcccg gacgcctgca tgaccggatg aaccgtcgat
      481 gaccctcagc accgatccgc tcgccgcctg gcaggcgctg cccgaacccg cccgcgtctt
      541 cgccgatgcg ctcgccgtgc tgtacgagcc gctcgccttc gcacactggc cggagctgta
      601 cgccgggctc ggcttcgcga agaagggagc ggccgcgcag aaggccgtgc aatccctgcc
      661 ggacaccctg cgcccgctgc tgcgcccgaa ggccggacgc gatccgggtc cgccggtgct
      721 cgatgcagcc gtgtccgaac gcctgatgcg ccggctcggc cgggagccgg aggcgctgcg
      781 gcgctttgcc gatcaggtgc tgcgtgcggt gccggccgca agtctgggcg cggcgctggc
      841 cggccgcgtc gatcccgccg tgttccagca gttacttgcg acgagcctga tcgtcaatca
      901 cagccgccgc gacctgcgcc tggccgtgca tgccaacgat ttcgatacgg ccctgcggat
      961 tgcgcgggag atgcagaatg gcgatccgga tcgctggttc atggagcgcc cgctgacgca
     1021 gctcgtgcgc tgcgcgtacg acgccgactg gctgttccag cgcgagccgc gcctgcgctg
     1081 gatgatgatc gaagactggc tcggcacgct ggtcggcgat ctggaagacc cgcgcgaggt
     1141 gcgcgccctg ctcgatcgtt gccggaacgc gccgcagcag caggccttgt tcttcgatcg
     1201 tctgcttgcc caggaact
//
LOCUS       DAOQJH010000762         1216 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_124232_length_1216_cov_3.530577, whole genome shotgun
            sequence.
ACCESSION   DAOQJH010000762 DAOQJH010000000
VERSION     DAOQJH010000762.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1216
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124232_length_1216_cov_3.530577"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..369
                     /locus_tag="PLQ18_14025"
     CDS             <1..369
                     /locus_tag="PLQ18_14025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018231790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HNE02821.1"
                     /translation="LDGAILGKPGTHERAREQLAQASGRELHFLTGLCLLDADGGGTQ
                     LEVAETVVRFRELLPQQIECYLRREQPYDCAGSFKCEGLGIALFEAILGADPDALVGL
                     PLIALVRMLDAEGVDVLTGF"
     gene            398..>1216
                     /locus_tag="PLQ18_14030"
     CDS             398..>1216
                     /locus_tag="PLQ18_14030"
                     /inference="COORDINATES: protein motif:HMM:NF039296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5924 family protein"
                     /protein_id="HNE02822.1"
                     /translation="MIDSPWLAERPRLRAAVRVLHFAWPYLAFAIGVASYALIERTDR
                     LAAWLVFTVFASWVWLLAEPWLKPMRGWRLSRRLARYATQAVHQETLFFGLPFLLRAT
                     DWGTGQALFTGAVAILCLVSLIDPLYFRYVCPRRWRFYAFHGLVAFLAMQAFAPVVFR
                     LDTVSGYRLALATGFVVAAQGFIRLRWGWRRWGRIVLLLGAVAALWAIRPWVPPITLW
                     MTSGAITQQFDRRQLRPGEVAVVLSPARLQQGVYAYMALRAPAGLKEPVFHVWRH"
BASE COUNT          133 a          439 c          434 g          210 t
ORIGIN      
        1 ctcgacggcg cgatcctcgg caagcccggc acgcacgaac gcgcccgcga acagctcgcg
       61 caggcgagcg gccgggagct gcacttcctg accggcctgt gcctgctcga tgccgatggc
      121 ggcggcacgc agctcgaagt cgccgaaacc gtcgtgcgct ttcgcgagct gctgccgcag
      181 cagatcgagt gctacctgcg ccgcgagcag ccctacgact gcgccggcag cttcaaatgc
      241 gaagggctcg gcatcgcgct gttcgaggcg atcctcgggg ccgacccgga cgcgctggtc
      301 ggcctgccgc tgatcgcgct cgtgcgcatg ctcgatgccg agggcgtcga cgtgctgacc
      361 ggcttctgat gccggtgcgg tccgccggaa ccggccggtg atcgactcgc cgtggctcgc
      421 cgaacgcccg cgcctgcgcg ccgcggtgcg cgtgctgcac ttcgcgtggc cgtatctcgc
      481 cttcgcgatc ggcgtcgcca gctacgcgct gatcgagcgc accgacagac tcgccgcctg
      541 gctggtgttc acggtgttcg cgagctgggt ctggctgctc gccgagccgt ggctgaagcc
      601 gatgcgcggc tggcggctgt cgcggcggct ggcgcgctat gcgacgcagg ccgtgcatca
      661 ggagaccctg ttcttcggcc tgcccttcct gctgcgcgcc accgactggg gcacgggtca
      721 ggcgctgttc accggcgcgg tggcgatcct ctgcctggtc tcgctgatcg acccgctgta
      781 cttccgctac gtgtgcccgc ggcgctggcg cttctatgcc ttccacggcc tcgtcgcctt
      841 cctggcgatg caggccttcg cgccggtggt gttccggctc gataccgtca gcggttaccg
      901 gctggcgctt gcgaccggct tcgtcgtcgc cgcgcagggc ttcatccgcc tgcgctgggg
      961 ctggcggcgc tggggcagga tcgtgctgct gctcggcgcg gtcgcggcgc tgtgggcgat
     1021 ccggccctgg gtgccgccga tcacgctgtg gatgaccagc ggcgcaatca cgcagcagtt
     1081 cgaccgcagg cagctgcgcc ccggcgaggt cgccgtcgtg ctgagcccgg cccggctgca
     1141 gcagggcgtc tatgcctaca tggcgctgcg cgcaccggcc ggcctcaagg agccggtgtt
     1201 ccacgtctgg cgccac
//
LOCUS       DAOQJH010000763         1210 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_125558_length_1210_cov_2.689177, whole genome shotgun
            sequence.
ACCESSION   DAOQJH010000763 DAOQJH010000000
VERSION     DAOQJH010000763.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1210
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125558_length_1210_cov_2.689177"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(324..566)
                     /locus_tag="PLQ18_14035"
     CDS             complement(324..566)
                     /locus_tag="PLQ18_14035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020158618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02823.1"
                     /translation="MTAQSLGTVLLGAALIHYLILLAWFAVFTGAHDALHRLHGRWFR
                     LPVERFDAIHYAAMAGYKLGVLLFFLVPGIALKLWG"
     gene            complement(772..>1210)
                     /locus_tag="PLQ18_14040"
     CDS             complement(772..>1210)
                     /locus_tag="PLQ18_14040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02824.1"
                     /translation="KVAVPPASVYEDPLLSEDPSRTQPFVPVVTQRVLPDPLLDEPRP
                     TDLYAARDASIETGERAADAVYSGAAHEHEPVLAPDALDEGISHAPGLEETPPTDPAS
                     VVGGSIAEDQHEPPHTVPPLAPIVPPVAPIAAPHQPERRRQPR"
BASE COUNT          190 a          427 c          437 g          156 t
ORIGIN      
        1 aagcgcagcc acgatcgctg gctcgatgcg gtctgcatcg acgactccgg aatccgcatc
       61 acggttcgct acgacctgtc cggcggcatc gattacctga tggacccggc ccgcgccacg
      121 aaggtgtacc gatacctgat cgaagactga gaccgatcac acgcaagtgc atgaatgcct
      181 ggcgcttcct tgacaagggc gatggactgg aaagcgaagc gatccggtgt ggccggcagg
      241 acgggagcga cggcgttccc ggtggcggcg acatgccgcg cagccggcgg gcaaggcggc
      301 ccgacgcccc tgtccctgcc gcctcagccc cagagcttca gcgcgatccc cggcaccaga
      361 aagaacagca gcacgccgag cttgtagccg gccatcgccg cgtaatggat ggcgtcgaag
      421 cgctcgaccg gcagccggaa ccagcggccg tgcaggcggt gcagcgcgtc gtgggcgccg
      481 gtgaacacgg cgaaccacgc gagcaggatc aggtagtgga tcagcgccgc gccgagcagc
      541 acggtgccga gggactgggc ggtcatggcc tgggctccgg tcgacgcggg gccgggtcag
      601 gctgctgcgg ttgcaggcgg cccggatcgg cagacaacgg cgaccggccc cgcagggtcg
      661 ccgttgccca cccgttccgc ggaaccggcc gtcgcggcac gcgcgcatcg gccggaagcg
      721 gaacgcacgc aaaccagaca tcaggcccca tgcaggcccc ctgcagatcg ctcagcgcgg
      781 ctggcggcga cgttccggct ggtgcggcgc tgcgatcggc gcgaccggcg gcacgatcgg
      841 cgccagcggc ggcaccgtgt gcggcggttc atgctggtcc tcggcgatcg agcccccgac
      901 cacgctcgcc ggatcggtcg gcggcgtttc ttcgagcccc ggcgcgtggc tgatgccctc
      961 atcgagcgca tcgggtgcga gcaccggctc gtgctcgtgc gccgcgccgc tgtagacggc
     1021 atccgcggcc cgctcgccgg tctcgatcga cgcatcgcgc gccgcgtaca ggtcggtcgg
     1081 gcgcggctcg tcgagcagcg ggtccggcag cacgcgctgc gtcacgaccg gcacgaaggg
     1141 ctgcgtgcgc gacgggtcct cgctcagcag cggatcctca tagaccgaag ccggcggcac
     1201 ggccaccttc
//
LOCUS       DAOQJH010000764         1196 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_128606_length_1196_cov_4.083260, whole genome shotgun
            sequence.
ACCESSION   DAOQJH010000764 DAOQJH010000000
VERSION     DAOQJH010000764.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1196
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128606_length_1196_cov_4.083260"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(376..852)
                     /locus_tag="PLQ18_14045"
     CDS             complement(376..852)
                     /locus_tag="PLQ18_14045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003149355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohemerythrin"
                     /protein_id="HNE02825.1"
                     /translation="MAHIIWSNDLSVGIEIIDSQHKRIVDYLNELDDARLKHSRDVVG
                     GVIEDLIDYTQSHFGFEETLMEEAGYPYVNAHKKIHELFIRRINEYHDRFKLGDDVTE
                     ELHRTLTNWLVNHIKREDNDYSSVVQVHTARGKVDLEPETSGWLGGALKRFFGKRK"
     gene            complement(1007..>1196)
                     /locus_tag="PLQ18_14050"
     CDS             complement(1007..>1196)
                     /locus_tag="PLQ18_14050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HNE02826.1"
                     /translation="EDIRRQGARSRQTTVGAALLIGAAVVYGLNSLNPAVVAAAPFVT
                     WALGLSGGALVLGAALRD"
BASE COUNT          178 a          376 c          418 g          224 t
ORIGIN      
        1 tgcatggcag gaaactccgg tgcgggtcgc gaggacgacg ccggtccgac gcgcgatggc
       61 ggggacggtt ctgcgcactt gcggactggc agcaaaaccg cagcggcata ccggccggcc
      121 ggctgcgagc gtcgtcgtcg cagcctttcg agttatacac aaaccggacg ccggcggccg
      181 gtttgtgcct gcagccgtgc atcgccgcag gcatggaaga caggtgtctg attgtcaagg
      241 taatccggca cgggcgggcg aggccggcac gcggtccggc tgcggcggga cgggcggggc
      301 tgacccgggg ttctccacaa aattatccac agggcggctg ccgcgcctgc cgctccggag
      361 cgggcgcggg cgcggctatt tcctcttgcc gaagaagcgc ttgagggcac cgccgagcca
      421 gcccgaggtt tcgggctcca gatccacctt gcccctggcc gtatgcacct gcacgacgct
      481 ggaatagtcg ttgtcctcgc gcttgatgtg gttcacgagc cagttggtca gcgtgcggtg
      541 cagttcttcg gtcacgtcat cgccgagctt gaagcggtcg tggtactcgt tgatgcggcg
      601 gatgaacagt tcgtggatct tcttgtgcgc gttgacgtag ggatagcccg cctcctccat
      661 cagcgtctcc tcgaaaccga agtggctctg cgtgtagtcg atcagatcct cgatgacccc
      721 gccgaccaca tcgcggctgt gcttcaggcg cgcatcgtcg agctcgttca ggtaatcgac
      781 gatgcgcttg tgctggctgt cgatgatctc gatgccgacg ctgagatcgt tgctccagat
      841 gatatgggcc atctttccac gctccggttg tgtgacggtg aggccgggag gctccggcat
      901 gagctgcgac ggtccgcgcc ggtatcccga ccggctgtgg ccgtcgcatg caatcgatgc
      961 ggttttacgg ttcgtacgct tgttcgtacg cgggtgacgg gccggttcag tcgcgcagcg
     1021 cggccccgag caccagcgcg ccgccgctca ggccgagcgc ccaggtcacg aacggtgcag
     1081 cggcgaccac cgccgggttg aggctgttca gaccgtagac gacagccgcg ccgatcagca
     1141 gcgcggcgcc gaccgtggtc tggcggctgc gtgcgccctg gcggcggatg tcctcg
//
LOCUS       DAOQJH010000765         1192 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_129509_length_1192_cov_4.321020, whole genome shotgun
            sequence.
ACCESSION   DAOQJH010000765 DAOQJH010000000
VERSION     DAOQJH010000765.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1192)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1192)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1192
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_129509_length_1192_cov_4.321020"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..408)
                     /locus_tag="PLQ18_14055"
     CDS             complement(<1..408)
                     /locus_tag="PLQ18_14055"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HNE02827.1"
                     /translation="MSDSFPFDQAATATLERILERLTEDGHDFTDYKSGTLRRQVLRC
                     AGGRTQAALSAYEARLAADPIERRRLAESFSIGVTHFFRDRRAFSALASALETTTRRL
                     PDGHTLRVWVPACCTGEEAYSLALMLSGLRTGSG"
     gene            complement(550..>1192)
                     /locus_tag="PLQ18_14060"
     CDS             complement(550..>1192)
                     /locus_tag="PLQ18_14060"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate binding domain-containing protein"
                     /protein_id="HNE02828.1"
                     /translation="AVPQGRLRVTMPPDLAVLMLVPMLARFGAGYPAVSVELDLSPHQ
                     VDLIAGGFDLAVRMGPLAEDASLVARRLGSFARGLYASPDYLARRGTPQAPEQLAGHD
                     ALLLAPQAGRPLPMSLQNGLQEWHGPWASRFGANSVELLVRMALAGAGLVAIDERFVR
                     PHLAAGTLLPVLPAWTLPPVTAWAVMPGRRLMPAKTRAFVEALIRTLADDKGD"
BASE COUNT          188 a          416 c          440 g          148 t
ORIGIN      
        1 gccactgccc gtgcgcagcc cggagagcat cagggccagc gaataggctt cctcgccggt
       61 gcagcaggcc ggcacccaga cccgcagcgt gtgtccgtcc ggcaggcgcc gggtggtcgt
      121 ttcgagggcg ctggccagcg ccgagaaggc gcgccggtcg cggaagaagt gcgtgacgcc
      181 gatcgagaag gattcggcca gccgccgccg ctcgatcggg tcggcagcga ggcgtgcttc
      241 ataggcgctg agcgccgcct gcgtccgccc gcccgcgcag cgcagcacct gccggcgcag
      301 cgtgccggac ttgtagtcgg tgaagtcgtg cccgtcttcg gtcaggcgct caaggatgcg
      361 ctccagggtg gccgtcgcgg cctgatcgaa cggaaacgaa tcggacatcg gtgcaactcc
      421 atgctgcaac ggcaagggcg gtgaaacggg aatatgacca gaagacagac aggcacgcga
      481 gacggcggat gttccgtcct gtccggtgca ctccggcgag cgctgccatg atcccgcgcc
      541 cgccgggttt cagtcaccct tgtcatcggc cagcgtccgg atcagggctt cgacgaaggc
      601 gcgcgtcttc gccggcatca ggcgccggcc cggcatcacg gcccaggccg tcaccggtgg
      661 cagtgtccag gccggcagca ccggcagcag ggtgccggcg gcgagatggg ggcgcacgaa
      721 gcgttcgtcg atcgcgacca gtccggcacc ggcgagcgcc atgcgcacca gcagttccac
      781 cgaattggcg ccgaagcgcg aggcccaggg gccgtgccat tcctgcaggc cgttctgcag
      841 gctcatcggc agcggccggc cggcctgcgg ggcgagcagc agcgcatcgt gaccggcgag
      901 ctgctccggg gcctgcggcg tgccgcgccg ggcgaggtag tcgggactcg cgtacaggcc
      961 gcgcgcgaag ctgccgagcc ggcgcgcgac gaggctggcg tcctcggcca gcggtcccat
     1021 gcgcacggcg agatcgaagc cgccggcgat cagatcgacc tggtgcggcg acagatcgag
     1081 ctcgacgctg accgccggat acccggcgcc gaagcgcgcg agcatcggca ccagcatcag
     1141 cacggccaga tccggcggca tcgtcacgcg cagccggccc tgcggcacgg cc
//
LOCUS       DAOQJH010000766         1192 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_129537_length_1192_cov_3.253298, whole genome shotgun
            sequence.
ACCESSION   DAOQJH010000766 DAOQJH010000000
VERSION     DAOQJH010000766.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1192)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1192)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1192
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_129537_length_1192_cov_3.253298"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..420)
                     /locus_tag="PLQ18_14065"
     CDS             complement(<1..420)
                     /locus_tag="PLQ18_14065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02829.1"
                     /translation="MDQPSLPAAGAGHLPPDPLNASLPETAGMPAVETAILNWLWRRE
                     HRIADTPARLMVALRDGALAELCAASLRRHFGRLDTAAAPPPELRLHDPVSPQLPQAD
                     ILIGPQHALIDALPTDEARQAQWLLDAPPVPVARRACQ"
     gene            complement(700..>1192)
                     /locus_tag="PLQ18_14070"
     CDS             complement(700..>1192)
                     /locus_tag="PLQ18_14070"
                     /inference="COORDINATES: protein motif:HMM:NF016259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF489 family protein"
                     /protein_id="HNE02830.1"
                     /translation="FAIDSPGIAEIYGGLPGLRRGLRLLASELRTPREMEIARYTVNL
                     LVLERKLRASPAMTERLRQGIRDTARRLDHFPVTHENIVAALGELYADTISTLTPRIM
                     VNGEQMHLSRRDNAARIRALLLAGIRAAWLWAQAGGGRLTLLLRRRALLAESQRLLRE
                     LGD"
BASE COUNT          165 a          391 c          469 g          167 t
ORIGIN      
        1 ctggcaggcg cggcgcgcga ccggcaccgg cggcgcatcg agcagccact gggcctggcg
       61 cgcctcgtcg gtgggcaggg cgtcgatcag cgcatgctgg gggccgatca ggatgtcggc
      121 ctgcggcagc tgcgggctga ccggatcgtg cagcctgagt tcgggcggcg gcgcggcagc
      181 ggtatcgagg cgcccgaagt gccggcgcag cgaggcggcg cacagctcgg ccagcgcgcc
      241 atcgcgcagc gcgaccatca gccgcgccgg ggtatcggcg atgcggtgct cgcgccgcca
      301 cagccagttc aggatggcgg tctcgaccgc tggcatgccg gcggtttccg gcagtgaggc
      361 gttcagcggg tccggcggca gatggccagc gccggcggct ggcagggacg gttgatccat
      421 gaatgaactc cgggcgcctt cctgcgaccg ggtgggcggt acgtccggga ttggcgtcgg
      481 gtggcggcga tgcccgcctt tcctggcatg actcgcgcgc acatgtatcc ggggggccgg
      541 ccggtgatga tgcgcagggc cgtcccgggc ttgcagtctc gtccgcggac tgatcagacc
      601 cggaccttag ccagcgcggg ggccgttgcg gcagggagca agaccggaac ctgcctgcga
      661 atattcggat tcgtccggtg gtcggaccac cgccgggatt caatccccga gttcgcgcag
      721 caggcgctgg gattcggcga gcagcgcccg gcggcgcagc agcagcgtca gccggccgcc
      781 gccggcctgc gcccatagcc aggcggcccg gatgccggcg agcagcagcg cgcggatgcg
      841 cgcggcgttg tcgcgccggg acaggtgcat ctgctcgccg ttgaccatga tccgcggcgt
      901 cagcgtcgag atcgtgtcgg cgtacagctc gccgagggcg gcgacgatgt tctcgtgcgt
      961 gaccggaaaa tgatcgaggc gacgggcggt gtcgcggatg ccctggcgca ggcgctcggt
     1021 catcgccggg ctggcgcgca gcttgcgctc cagcacgagc agattgacgg tgtaacgggc
     1081 gatttccatc tcgcgcggcg tgcgcagctc cgaagccagc agccgcagac cgcggcgcag
     1141 gccgggcagg ccaccataga tctcggcgat gccgggcgag tcgatcgcga ag
//
LOCUS       DAOQJH010000767         1184 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_131456_length_1184_cov_2.509300, whole genome shotgun
            sequence.
ACCESSION   DAOQJH010000767 DAOQJH010000000
VERSION     DAOQJH010000767.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1184)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1184)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1184
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_131456_length_1184_cov_2.509300"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="PLQ18_14075"
     CDS             complement(<1..191)
                     /locus_tag="PLQ18_14075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE02831.1"
                     /translation="MNPEHARRPRLLVVDDEADNREFFAETGDLAGYAVASLGDVREL
                     ESALQPAPDVILLDLIMPHL"
     gene            complement(287..>1184)
                     /locus_tag="PLQ18_14080"
     CDS             complement(287..>1184)
                     /locus_tag="PLQ18_14080"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNE02832.1"
                     /translation="GIIELLPLIRQSAGSGVRIVLHGFEPAPQPPLRACVDAGGLGNA
                     LLNLVINARDAMPGGGTLPLEVQLQTLPAGAVPELAAGTWIVIAVTDTGCGMSPAVRA
                     RAFDPFFTTKHRGQGTGLGLAMVLGYARQSGGNATLAGAAGGGTTVRLYLPAVAPMSP
                     RPEAALPVPGVAAPAGQGERVLLVDDESGLLELGREWLEADGYRVDTCSDPQQALARL
                     QAGGHALLVTDVTMPGPLDGCALAAHACRQQPGLRVLLISGYADDDALAELSRWPLLL
                     KPFTAVELLQAVRRRLDQPGGG"
BASE COUNT          173 a          431 c          421 g          159 t
ORIGIN      
        1 aggtgcggca tgatcaggtc gagcaggatc acgtccggcg ccggctgcag cgcggattcc
       61 agctcgcgga catcgccgag gcttgcgacc gcgtagccgg cgagatcgcc ggtctcggcg
      121 aagaactcgc ggttgtcggc ttcgtcatcg acgaccagca ggcgcgggcg gcgggcgtgt
      181 tcagggttca tcggttttcc atcctgttgg cgatcgggat tccccgcccc gtatcccgga
      241 gcgggttcgg cggctgtcac gaaatcttca tgcacgaatc ccggcctcag ccgcccccgg
      301 gctgatccag cctgcgccgc accgcctgca gcagttcgac cgcagtgaac ggcttgagca
      361 gcagcggcca gcgcgacagc tcggccagcg cgtcgtcatc ggcgtagccc gagatcagca
      421 gcacccgcag cccgggctgc tgccggcagg catgcgcggc cagtgcgcag ccatcgagcg
      481 gacccggcat cgtcacgtcg gtgacgagca gcgcgtgccc gccggcctgc agccgtgcca
      541 gggcctgctg cggatcgctg caggtatcga cccggtagcc atcggcctcc agccattcgc
      601 gcccgagttc gagcaggccg gattcatcat cgaccagcag cacgcgctcg ccctggccgg
      661 ccggggcggc cacccccggc accggcagcg ctgcctccgg gcgcggactc atcggtgcca
      721 cggccggcag atacagccgg accgtcgtgc cgccgcccgc agcgccggcg agcgtggcat
      781 tgccgccgga ctggcgggcg taaccgagca ccatcgccag cccgagcccg gtgccctggc
      841 cgcggtgctt ggtcgtgaag aacggatcga aggcccgcgc ccgcaccgcc ggcgacatgc
      901 cgcagccggt atcggtgacg gcgatcacga tccaggtgcc ggccgccagt tcgggcacgg
      961 cgccggcggg cagggtctgc agttgcacct ccaggggcag cgtgccgccg cccggcatcg
     1021 catcgcgggc gttgatcacg aggttcagca atgcgttgcc gaggccgccg gcatcgacgc
     1081 aggcccgcag cggcggctgc ggcgcgggtt cgaagccatg cagcacgatg cgcacgcccg
     1141 agccggccga ctggcggatc agcggcagca gctcgatgat tccg
//
LOCUS       DAOQJH010000768         1153 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_138744_length_1153_cov_3.827869, whole genome shotgun
            sequence.
ACCESSION   DAOQJH010000768 DAOQJH010000000
VERSION     DAOQJH010000768.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1153
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_138744_length_1153_cov_3.827869"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(8..211)
                     /locus_tag="PLQ18_14085"
     CDS             complement(8..211)
                     /locus_tag="PLQ18_14085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02833.1"
                     /translation="MCPDVRPHPPAVLPLAAELADFIGGAVSISLGSCGAGLVPNLTR
                     GLGCRVSADRRRLRVWLAAGRRS"
     gene            685..>1153
                     /locus_tag="PLQ18_14090"
     CDS             685..>1153
                     /locus_tag="PLQ18_14090"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNE02834.1"
                     /translation="MRNDANGRTQAEALRSICLRVLPIWIRHIESVRTQSETAVSTLI
                     ARFTGLMDTLGVAIASAESGADGSREALSGLLRTGERRLLDLLDTLRAANDNRDGALA
                     PVLGLGARTDELLEMARTVTTISGQTRLLSLNATIEAVHAGEHGQGFEVVAREV"
BASE COUNT          212 a          381 c          396 g          164 t
ORIGIN      
        1 gacaagctca cgagcgtcgg ccggccgcga gccagacgcg cagccggcgg cggtccgccg
       61 atacccggca gccgaggccg cgcgtcaggt tcggcaccag cccggcaccg cagctgccga
      121 ggctgatcga taccgctccg ccgatgaaat ccgccagttc ggcggcgagc ggcagcacgg
      181 caggcgggtg cgggcggaca tcgggacaca tggcggctac cgcttggtcg gcttttctgg
      241 cgacagcgta ggtgctggcc tgcgcgcctg cgccgggtcg cggcgcaggc ggctgtcagc
      301 aaatcttaca catgacaaaa attgttccgc gacgcttgcc gtgcttgtgc gccgatatag
      361 tgccggacaa acagattgcg acctgcccgc agaaacatcc gggggtcgtg caatgcagca
      421 ggacatgcag atgaattcgt gatttccgga aaaacccggg ctgccattca gaaaaccggg
      481 acggagccga tgaagccgtc acggcatcga ctgcatgccg gcaatccctg atctcaggcg
      541 tttcagatat gttccggacg aataagcatg agcacgggtg gagccattcg gacattgcag
      601 cactggttca aaaagcggcc ggccccggag gccggcgaat tccgggaccg gcacgatgcg
      661 tcagccatga cgatcggaca tgacatgcga aatgatgcga acggccggac gcaggccgag
      721 gcactgcgca gcatctgcct gcgcgtgctg cccatctgga tccggcacat cgagagcgtg
      781 cgcacgcaat cggaaacggc ggtatcgacg ctgatcgcgc gcttcaccgg gctgatggac
      841 acgctcggcg tggcgatcgc ctcggccgag tccggcgccg acggcagccg cgaggcgctc
      901 tccgggctgc tgcgcaccgg cgagcgccgt ctgctcgacc tgctcgacac gctgcgcgcc
      961 gccaacgaca accgcgatgg cgcgctcgca ccggtgctcg ggctcggcgc gcgcaccgac
     1021 gaactgctgg agatggcgcg cacggtcacg accatctccg gccagacgcg gctgctgtcg
     1081 ctgaacgcga cgatcgaggc cgtgcacgcc ggcgagcacg gccagggctt cgaggtcgtc
     1141 gcccgcgagg tgc
//
LOCUS       DAOQJH010000769         1138 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_142538_length_1138_cov_4.345337, whole genome shotgun
            sequence.
ACCESSION   DAOQJH010000769 DAOQJH010000000
VERSION     DAOQJH010000769.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1138
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_142538_length_1138_cov_4.345337"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..307)
                     /locus_tag="PLQ18_14095"
     CDS             complement(<1..307)
                     /locus_tag="PLQ18_14095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018954270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNE02835.1"
                     /translation="MAALGRWALTVFARTGRASLMLLAILAGLPSVLPRPRLLVRQLY
                     SVGVLSLPIIVVAGLFVGMVLALQGYTTLADFGAAGAPGTVVALSLVRELGPVVAALL
                     "
     gene            complement(307..1098)
                     /locus_tag="PLQ18_14100"
     CDS             complement(307..1098)
                     /locus_tag="PLQ18_14100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011629959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNE02836.1"
                     /translation="MRGLSFHRGERMIFNDLNLEIRRGQVTAIMGPSGTGKTTLLRLI
                     GGQLRPAAGSVVLDGHEVPALRRRELYALRRRIGMLFQSGALFTDLSVFENVAFPLRE
                     HYPRLPARLLRTLVLMKLEAVGLRGARDLKPAELSGGMARRVALARAIALDPMLMLYD
                     EPFTGQDPISMGVLVQLIRTLNDALGMTSVVVSHDVAEVCSIADYVYLISGGRVVASG
                     TPAELRASGSDWVGQFMHGLPDGPVPFHYPARDYASELLDGGGRS"
BASE COUNT          214 a          415 c          374 g          135 t
ORIGIN      
        1 agagcagcgc ggcgaccacc ggcccgagtt cgcgcaccag cgacagcgca accaccgtgc
       61 cgggcgcgcc ggccgcgccg aaatccgcca gcgtcgtata gccctgcagc gccagcacca
      121 tgccgacgaa caggccggcg acgacgatga tcggcagact cagcacgccg accgaataca
      181 actggcgcac cagcagccgc ggccgcggca gcaccgaagg caggccggcg aggatcgcga
      241 gcagcatcag gctggcacgc ccggtgcgcg cgaacaccgt cagcgcccag cgcccgagcg
      301 cggcgatcaa gaccgtcctc cgccatcgag cagctcgctc gcgtagtcgc gcgccggata
      361 atgaaacggc accggcccgt cgggcaggcc gtgcatgaac tgcccgaccc agtccgaacc
      421 ggaagcccgc agctcggccg gcgtgccgct tgcgaccaca cgcccgcccg agatcaggta
      481 cacgtagtcg gcgatcgagc agacctcggc cacatcatgc gagacgacga cgctggtcat
      541 gccgagcgcg tcgttgagcg tgcggatcag ctgcaccagc acgcccatcg agatcgggtc
      601 ctgacccgtg aacggttcgt cgtagagcat cagcatcggg tcgagtgcga tggcacgcgc
      661 gagcgccaca cgccgggcca tgccgccgga gagctccgcc ggcttcaggt cacgcgcgcc
      721 gcgcaggccg acggcttcga gcttcatcag caccagcgtg cgcagcagcc gcgccggcag
      781 ccgcggatag tgctcgcgca gcggaaacgc gacgttctcg aacaccgaca gatcggtgaa
      841 cagcgccccg ctctggaaca gcatgccgat gcgccggcgc agcgcgtaca gctcgcgccg
      901 cctaagcgcc ggcacctcgt gaccgtcgag cacgacgctg ccggctgccg gccgcagctg
      961 accgccgatc aggcgcagca gcgtggtctt gccggtgccg ctcggcccca tgatcgcggt
     1021 gacttgaccg cgccgaatct ccagattcag gtcattgaag atcatccgct cgccgcggtg
     1081 gaaggataat ccgcgcaccg atactaaagg cgagtcatcg ggtcgaggcg tttctgga
//
LOCUS       DAOQJH010000770         1129 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_145076_length_1129_cov_2.190875, whole genome shotgun
            sequence.
ACCESSION   DAOQJH010000770 DAOQJH010000000
VERSION     DAOQJH010000770.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1129
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_145076_length_1129_cov_2.190875"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1129
                     /locus_tag="PLQ18_14105"
     CDS             <1..>1129
                     /locus_tag="PLQ18_14105"
                     /inference="COORDINATES: protein motif:HMM:NF024755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HNE02837.1"
                     /translation="PDEIEVDGKLRRFASNGRRNDDAGWYLLHVDGIPAGSFGDWRMS
                     LTQSWRAHLGRDLTPAEEAEHRAKVEAMRREREAEEARRQAEAAATAAVILKEATPAT
                     DKHAYLIRKGIKANGALLHQGKLVIPMRSGGKVHSLQFIGTDGEKRFLTGGRVTGCYF
                     SIGSARDATALCIVEGFATGASIQEATGYPVAVAFNAGNLLPVAKALREKFPDLPLIL
                     CADDDSRTEGNPGLTKATEAAKAINGRLIVPDFGPDRPDGASDFNDLARLRGDDVVRR
                     AFLAGTWPGEGLDIAGCLSTPAPPVRWFITERMPAGRAGLLAAVGGSSKTRTLFHLAV
                     GACIGRLPWPWEVATTGRAVLLLAEDTAADAHHALSLIGRML"
BASE COUNT          212 a          370 c          375 g          172 t
ORIGIN      
        1 ccggacgaga tcgaggtcga cggcaagctg cggcgcttcg ccagcaacgg acggcgcaat
       61 gacgatgcgg gctggtatct gttgcacgtc gacggcattc ccgccgggag tttcggtgac
      121 tggcgcatga gcctgacgca atcctggcga gcccatcttg gccgggacct gacgccggca
      181 gaggaagccg agcaccgggc caaggtcgaa gccatgcgac gcgagcgcga agccgaggaa
      241 gcgcggcgac aggccgaagc cgcagcgaca gcggcggtga tcttgaagga agcgacaccg
      301 gcgaccgaca aacacgccta tttgattcgc aagggcatca aggcgaacgg cgcgctactg
      361 catcaaggaa agctcgtaat cccgatgcga tccggcggca aggtgcactc gctgcaattc
      421 atcggcaccg atggcgaaaa acgcttcctg acaggcggac gcgtgacggg ctgttacttc
      481 agcatcggca gcgcaagaga cgcaacggcg ctgtgcatcg tcgaaggctt cgcaaccgga
      541 gcgagcattc aagaagcgac cggctacccg gtagccgtgg ccttcaacgc cggcaatctg
      601 ctccctgttg cgaaggccct gcgggagaag ttccccgacc tgccgctgat cctgtgcgcc
      661 gacgatgatt cccgcaccga agggaatccg ggcctgacga aggcgactga ggccgcgaag
      721 gccatcaacg gccggctgat agttcccgac ttcggccccg accgaccgga cggggccagc
      781 gacttcaacg acctggcgcg cctgcgaggc gatgacgttg tacgccgcgc ctttctggcg
      841 ggcacctggc ccggcgaagg actggacatt gctggatgtc tttcgacgcc tgcacccccg
      901 gtgcgctggt tcatcaccga gcgcatgccc gccggtcggg ccggactgct tgcggccgtt
      961 ggtggatcat cgaaaacgcg cacgctgttt cacctcgccg tcggcgcctg catcggccgc
     1021 ttgccttggc cctgggaagt ggcgacaacc ggccgcgctg tgctgctgct ggccgaggac
     1081 accgccgccg acgctcacca tgccctgagc ctgatcggtc gcatgctca
//
LOCUS       DAOQJH010000771         1111 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_149749_length_1111_cov_3.355114, whole genome shotgun
            sequence.
ACCESSION   DAOQJH010000771 DAOQJH010000000
VERSION     DAOQJH010000771.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1111
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_149749_length_1111_cov_3.355114"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..413
                     /locus_tag="PLQ18_14110"
     CDS             <1..413
                     /locus_tag="PLQ18_14110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02838.1"
                     /translation="VRVPAESGAANYLLLVLLAPLEREGPFHTAPEILGRASEIAFHA
                     TFIQEMQGLAERAAAPIWQRYSDPAARRRYHLLAAGPELTELPQPSKLNADRAFLDRL
                     FEAGRSRAEAWLAADGAALGRGRGIDLADVFGEL"
     gene            complement(442..882)
                     /locus_tag="PLQ18_14115"
     CDS             complement(442..882)
                     /locus_tag="PLQ18_14115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013816950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNE02839.1"
                     /translation="MQEYRHVLFATDLSPKAAEVGRHAQGLAARYGARLSFVHVIEEV
                     NVSAGYELMPLLPEMPDEALTRESKAALARLAAELGLPEAVQTVLTSVSTKAGILDTA
                     AELGVDLIVVGSHGRHGLALLLGSTADAVLHGARCDVLAVRLPD"
BASE COUNT          153 a          402 c          392 g          164 t
ORIGIN      
        1 cggtgcgcgt gccggccgag agcggcgcgg ccaactacct gctgctggtg ctgctggcgc
       61 cgctcgaacg cgaagggccg ttccacaccg cgcccgagat cctcggtcgc gccagcgaga
      121 tcgccttcca cgccacgttc atccaggaaa tgcagggcct cgccgaacgc gcggccgcgc
      181 cgatctggca gcgttacagc gatccggcgg cccgccggcg ctatcacctg ctcgccgccg
      241 gccctgaact gaccgagctg ccgcagccga gcaagctcaa cgccgatcgc gccttcctcg
      301 accggctgtt cgaggccggt cgcagccggg ccgaggcctg gctcgccgcg gacggcgccg
      361 cccttggacg cgggcgcggc atcgatctcg ccgacgtgtt cggcgagctt tgaatgcctg
      421 cacccatgcc cgccgcgtga ttcagtccgg caggcgcacg gcgagcacgt cgcagcgggc
      481 gccgtgcagc acggcatcgg cggtcgagcc gagcagcagc gcaagcccgt ggcgaccgtg
      541 gctgccgacc acgatcaggt ccacgccgag ttcggcggcg gtgtcgagga tgccggcctt
      601 ggtgctgacc gaggtcagca ccgtctgcac ggcctccggc aggccgagtt cggcagccag
      661 ccgggcgagt gcggcctttg attcgcgggt cagcgcctcg tcgggcatct ccggcagcag
      721 cggcatcagc tcgtagccgg cactgacgtt gacttcctcg atgacgtgca cgaacgacag
      781 gcgcgcgccg tagcgggcgg ccagaccttg cgcgtgccgg ccgacttcgg cggccttcgg
      841 cgacaggtcg gtggcgaaca gtacgtggcg gtactcctgc atggcacggt cctccgggca
      901 gatcggtggg ggtttccgat ccgtccgcgg gcctcaggcg acggccgcga cgtggccgtt
      961 cgccaccagg cgccggcgtt cctggccgac ccactcgcgg atgtgctcgc cgagctgcac
     1021 gacgatgcgc ttcatgcatc gccgccgcgc gacctcgccg ccggtgactt cggacggatc
     1081 gggaaaatcc cagtgtatgt gacggtactc g
//
LOCUS       DAOQJH010000772         1105 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_151432_length_1105_cov_4.268571, whole genome shotgun
            sequence.
ACCESSION   DAOQJH010000772 DAOQJH010000000
VERSION     DAOQJH010000772.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1105
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_151432_length_1105_cov_4.268571"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(80..427)
                     /locus_tag="PLQ18_14120"
     CDS             complement(80..427)
                     /locus_tag="PLQ18_14120"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HNE02840.1"
                     /translation="MTELPVFDPQEALRVTGNRAAIATHLLGLFLNELPEARLVITDL
                     AADGEREALRARVHKLAGSAMYCGAVRLLAVSRALEAAIEDGAADADIETGTRSVLDE
                     IGRFIAAAPKMPG"
     gene            complement(561..>1105)
                     /locus_tag="PLQ18_14125"
     CDS             complement(561..>1105)
                     /locus_tag="PLQ18_14125"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE02841.1"
                     /translation="LQAWQIARPPVSREVLPVLDALLARHAGTPLPDQASTTVPAAHS
                     TPGTQGLHVLLADDNLINRQLARLFLQQLGAQVDEAANGLEAVQRCAGHDYDVVLMDI
                     QMPELDGLSATRRIRAAGPNRDTPIVALTAEILPSDAGRYRAAGIDDQVIKPVTEEAL
                     VATLLKWGNRCHLPDPQVQA"
BASE COUNT          148 a          383 c          395 g          179 t
ORIGIN      
        1 tcatggctcc atcctctcaa aggcgggagc ctccacgaaa cccggggcgg ttcactgcgc
       61 agcggagcgt tccgccgatt catccgggca tcttcggcgc agccgcaatg aatctgccga
      121 tctcgtccag caccgagcgc gtgccggtct cgatgtccgc gtcggcggca ccgtcctcga
      181 tggcggcttc cagggcccgg ctgacggcga gcagccgcac cgcgccgcag tacatcgccg
      241 aaccggcgag cttgtgcacg cgcgcgcgca gcgcctcgcg ctcgccgtcg gcggccagat
      301 cggtgatcac gagcctggcc tcgggcagtt cgttcaggaa caggccgagc agatgcgtgg
      361 cgatcgcggc acggttgccg gtcacgcgca gggcttcctg cgggtcaaag acgggaagtt
      421 cagtcacgtg cgctccttgg ttgtcgcatt ccatggcatg gctccggtgt tctctgctcc
      481 gcggcgcgcc tccggcggct gcgggtgcag tggtgggggt tcagaagccg tccggcggcc
      541 cgtcgggtcg cgcctgcact tcaggcctgc acctgcggat cgggcaggtg gcagcggttg
      601 ccccatttca gcagcgtcgc gaccagggct tcctcggtga ccggcttgat gacctgatcg
      661 tcgatgccgg cggcacgata acgcccggcg tcgctcggca ggatctcggc ggtcagcgcg
      721 acgatcggcg tatcgcggtt cgggccggcc gcgcggatgc gccgcgtcgc cgacaggcca
      781 tcgagttcgg gcatctggat gtccatcagc accacgtcgt agtcgtgtcc ggcgcagcgc
      841 tgcacggcct ccagcccgtt ggccgcctcg tcgacctgcg caccgagctg ctgcaggaac
      901 agccgggcga gctggcggtt gatcaggttg tcatcggcga gcagcacgtg cagcccctgg
      961 gtaccgggcg tgctgtgcgc cgccggcacc gtcgtcgaag cctgatccgg cagtggcgtg
     1021 ccggcgtggc gggcgagcag cgcatcgagc accggcagca cctcgcgcga caccggcgga
     1081 cgggcgatct gccaggcctg cagcc
//
LOCUS       DAOQJH010000773         1098 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_153593_length_1098_cov_2.196548, whole genome shotgun
            sequence.
ACCESSION   DAOQJH010000773 DAOQJH010000000
VERSION     DAOQJH010000773.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1098
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_153593_length_1098_cov_2.196548"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(197..883)
                     /locus_tag="PLQ18_14130"
     CDS             complement(197..883)
                     /locus_tag="PLQ18_14130"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02281 family clan AA aspartic protease"
                     /protein_id="HNE02842.1"
                     /translation="MQQSLSAPLAGARFRRRAMQMLALYMAAGTAAWAAPQVRVLALF
                     EGKALLEIDGSRRVLAAGAVSPEGVRVVSASSRAVLVEIDGRQQSLGLGAAGRFGTAP
                     AGLEVRLVRDGTGGYRSPGLVNGRSVEWLVDTGATAVALSGADADRLGLPWRSAGRPV
                     GIATAQGTKTGHRIVIDSLQIGDLRVSSVDAVVLDGDYPRGPLLGMNVLQRLTMRTEG
                     DLLILQQTQP"
BASE COUNT          155 a          419 c          387 g          137 t
ORIGIN      
        1 gaaaaagccc tgcagcgcgc cgaggccacc ggccgaggcc ccgagcgcaa cgatcgggaa
       61 acggcctgcc tcgctcatcg gtgcctcgcg tccgtcatcc acggcgacct ccgcgctcac
      121 gcctgcagcc tccggacacc gcagtgccgg aacgggcggg cacgagcggc tgcggacagg
      181 accggtggcg ggtcatttac ggttgcgtct gctgcaggat cagcagatcc ccttcggtgc
      241 gcatcgtcag acgctgcagc acgttcatgc cgagcagcgg cccgcgcgga tagtcaccgt
      301 cgagaacgac ggcatcgacg ctgctgacgc gcaggtcgcc gatctgcagg ctgtcgatga
      361 cgatgcggtg cccggtcttc gtgccctggg cggtggcgat gccgaccggc cggccggcgc
      421 tgcgccatgg caggccgagg cggtcggcgt cggccccgct gagcgccacc gcggtggcgc
      481 cggtatcgac cagccattcg accgatcggc cgttgaccag gccggggctg cggtagccgc
      541 cggtcccgtc gcgcaccagc cgcacctcca gcccggccgg cgccgtcccg aagcgaccgg
      601 cggccccgag tccgagcgac tgctgacggc cgtcgatctc gacgagcacg gcccgcgaac
      661 tggccgacac cacccgcacg ccctcgggcg acacggcacc ggcggcgagc acgcgccggc
      721 tgccgtcgat ctccagcagg gccttgcctt cgaacagcgc cagcacgcgc acctgcggtg
      781 cggcccaggc cgcggtgccg gccgccatgt acagcgcgag catctgcatg gcacggcgcc
      841 ggaagcgggc gccggccagc ggcgccgata gggattgctg catgatcttg ggttctccgg
      901 accggtcgga cctgcgttcc acgcactgcc gtcgccccgt cggcggcagc caccggtcct
      961 gccgctaaac ataagccacc tgccgcggct cgggcagtct gtcctgtgca gccggcctgt
     1021 gcctgcggca cagtcggacc ggccggcgcg tgacgggccg gcccgcggca ggccgccggc
     1081 acgcacgtga caagccgc
//
LOCUS       DAOQJH010000774         1078 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_159561_length_1078_cov_3.503421, whole genome shotgun
            sequence.
ACCESSION   DAOQJH010000774 DAOQJH010000000
VERSION     DAOQJH010000774.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1078
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_159561_length_1078_cov_3.503421"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..810
                     /gene="sucD"
                     /locus_tag="PLQ18_14135"
     CDS             <1..810
                     /gene="sucD"
                     /locus_tag="PLQ18_14135"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011869914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HNE02843.1"
                     /translation="MCREYANGRNAFVAGVNPKKAGEAFEGVPIYATVKEAKEATGAT
                     VSVIYVPPPFAAAAIDEAVDAGLELVVCITEGIPVRDMIRTRDRMKGRRTLLLGPNCP
                     GVITPDEIKIGIMPGHIHRKGRIGVVSRSGTLTYEAVGQLTELGLGQSTAVGIGGDPV
                     NGLKHVDVLRMFNDDPDTDAVIMIGEIGGGDEEVAAQWAKVHMRKPVVGFIAGVTAPP
                     GKRMGHAGAIISGGKGTAGEKVAALKAAGIEVADSPADLGAAMQRAIARRS"
BASE COUNT          185 a          348 c          385 g          160 t
ORIGIN      
        1 atgtgccgcg agtacgcgaa cggccgcaac gccttcgtcg ccggcgtgaa cccgaagaag
       61 gccggcgagg cgttcgaggg cgttccgatc tacgcgacgg tgaaggaggc gaaggaggcg
      121 accggcgcga cggtgagcgt catctacgtg ccgccgccgt tcgcggccgc ggcgatcgac
      181 gaggcggtcg acgccggcct cgagctggtc gtctgcatca ccgagggcat tccggtgcgc
      241 gacatgatcc gcacgcgcga ccgcatgaag ggacgcagga cgctgctgct cggccccaac
      301 tgcccgggcg tgatcacgcc cgacgagatc aagatcggca tcatgccggg ccacatccac
      361 cgcaaggggc gcatcggcgt cgtgtcgcgc tcggggacgc tcacctacga ggcggtcggc
      421 cagctgaccg agctcgggct gggccagtcg accgcggtcg ggatcggcgg cgatccggtg
      481 aacggcctta agcacgtcga cgtgctgcgc atgttcaacg acgacccgga caccgacgcc
      541 gtgatcatga tcggcgagat cggcggcggc gacgaggaag tggccgcgca gtgggcgaag
      601 gtgcacatga ggaagccggt ggtcggcttc atcgcgggcg tcaccgcgcc gccgggcaag
      661 cggatgggcc acgccggcgc gatcatctcc ggcggcaagg gcacggccgg tgaaaaggtc
      721 gcggccctga aggccgccgg catcgaagtc gccgacagcc ccgccgacct cggcgccgcc
      781 atgcaacgag cgatcgctcg ccgcagctag tgttttgcca caacttagtt ttcgggtaga
      841 cgcacttgcg ttttcaacgg acgtcgtcga ctttcagttg cgcaaatcaa tcgcaacctc
      901 gagatcggta gactgcctga agcctcgcct tgttttcgcc ttggggcggc gcgttggtgt
      961 accgctggcg cgtgcaggtc tgaaagaccg acccgaacat agcccagggc tgcggtggcg
     1021 aggcggtaag gtcgggcttt cggcccgaac aatccgacga gccgccgctg ccctgggt
//
LOCUS       DAOQJH010000775         1076 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_160148_length_1076_cov_4.087169, whole genome shotgun
            sequence.
ACCESSION   DAOQJH010000775 DAOQJH010000000
VERSION     DAOQJH010000775.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1076
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_160148_length_1076_cov_4.087169"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="PLQ18_14140"
     CDS             <1..216
                     /locus_tag="PLQ18_14140"
                     /inference="COORDINATES: protein motif:HMM:NF015440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-alpha domain-containing protein"
                     /protein_id="HNE02844.1"
                     /translation="LYRVCEAGDFGPQSPLIPEGSPDPQAPSVQAVWDAWIDGTDPAT
                     LARLAGWPTLAPVWRQAFAQRLAHRRG"
     gene            239..856
                     /locus_tag="PLQ18_14145"
     CDS             239..856
                     /locus_tag="PLQ18_14145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015433808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HNE02845.1"
                     /translation="MNESAAAQAVPQGFGPSTAQRLPVPEREAMSEAQRAAAQAIIDG
                     PRKALFGPFVPLLQTPQLMECLGKTGETLRFGGSLPDRVRELAICIVARETHNQFEWL
                     MHAPLALKAGVAAEVIDCLLQGRRPRDLAPDETCAVDFVTELMHRHGVSDATYAEATG
                     LFGEPGTVELTALTGYFVSVCWIMNVARTPGPQDSPAGRLSAFPA"
BASE COUNT          158 a          383 c          371 g          164 t
ORIGIN      
        1 ctgtaccgcg tgtgcgaagc cggcgatttc ggtccgcagt ccccgctgat ccccgagggc
       61 agccccgatc cgcaggcccc gagcgtgcag gcggtctggg atgcgtggat cgacggcacc
      121 gaccccgcca ccctcgcccg cctcgccggc tggccgacgc tcgccccggt ctggcggcag
      181 gcgttcgcgc agcgactggc acatcggcgc ggctgaacct tcaccgggag aatccggcat
      241 gaacgaatca gcggctgcgc aggccgtccc ccagggtttc gggccttcga cggcgcaacg
      301 cctgccggtc cccgagcgcg aagccatgtc cgaagcccag cgcgcggcgg ctcaggccat
      361 catcgacggt ccgcgcaagg ccctgttcgg cccgttcgtg cccttgctgc agaccccgca
      421 gctgatggaa tgcctcggca aaaccggaga aacgctgcgc ttcggcggca gcctgcccga
      481 tcgcgtgcgc gaactggcga tctgcatcgt ggcccgggaa acgcacaacc agttcgaatg
      541 gctgatgcat gcgccgctgg cgctcaaggc cggagtggca gccgaggtga tcgactgtct
      601 gctgcaaggc aggcgcccgc gcgatctggc gcctgatgaa acctgcgcgg tcgatttcgt
      661 gaccgaactg atgcaccggc acggcgtcag cgatgcgact tacgccgaag cgaccggctt
      721 gttcggcgaa ccgggtaccg tcgaactgac ggcactcacc ggttatttcg tgagcgtgtg
      781 ctggatcatg aacgtcgccc gcacgcccgg cccgcaggat tccccggccg gccggctgtc
      841 cgcgtttccg gcctgagcga cggactgcgc cccgccggcc cgcaatgcaa cggcccgcca
      901 gcaggcgggc cgtccggatc gaagagccgg cgaagcgtgt tcagcaggtg ccggtctggc
      961 tgttgcggcc gccgcgcgga ttgagcggcg aatcgctgtc cagcgcgcag gtgcgctggc
     1021 catcgctgct gcgcttcgcg tcggccaccg gcatgtgcgg ctgcatgtgc cggtcg
//
LOCUS       DAOQJH010000776         1067 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_163146_length_1067_cov_1.967391, whole genome shotgun
            sequence.
ACCESSION   DAOQJH010000776 DAOQJH010000000
VERSION     DAOQJH010000776.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1067
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_163146_length_1067_cov_1.967391"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            120..>1067
                     /locus_tag="PLQ18_14150"
     CDS             120..>1067
                     /locus_tag="PLQ18_14150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNE02846.1"
                     /translation="MSRPIGAASWPVAMLSALRALPRTVWLLGCVSLLNDAASDLVYP
                     LLPLYLATLPGAGAQALGLIEGCAEAVASLLKLASGVLADRHGSARPWVIGGYGLAAL
                     TLPLIALASGWPMVLALRVGDRIGKGLRSSPRDALLARSVAPAQRGLAFGVHRAMDNA
                     GAVAGPLLAAALLGLGVPLRELFLWTAMPGALTLWLIWRVPETPVIAAPASTAPAPAA
                     GAPLPPALRRYLAIYALFSLGNASNLFLLWRAHELGLADAALPLLWALVSAVAMLGSA
                     PLSGLSDRIGRRRLIAGGWTLFALVYALLGWPGLPPAGLW"
BASE COUNT           99 a          388 c          395 g          185 t
ORIGIN      
        1 tgcatgcacc ggcagctttt ttcaagctac tgactgtact ggagattcct gttgaaccac
       61 cgtctcgttc ctgatccggt ctccgggcac cggcctgtcc acccgcctcg ccccgcgaaa
      121 tgtcgcggcc gatcggcgcg gcttcgtggc cggtcgcgat gctgagcgcg ctgcgggcgc
      181 tgccgcgcac ggtctggctg ctcggctgcg tgagcctgct caacgacgcc gccagcgacc
      241 tcgtctatcc gctgctgccg ctgtatctgg cgacgctgcc cggggccggt gcacaggcgc
      301 tcgggctgat cgaaggctgc gccgaagcgg tcgcgagcct gctgaagctc gcctccggcg
      361 tgctggccga tcgccatggc agcgcacgac cgtgggtgat cggcggttat ggcctggcgg
      421 cactgacgct gccgctgatc gcgctggcga gcggctggcc gatggtgctg gcgctgcgcg
      481 tcggggatcg catcggcaag ggcctgcgca gcagcccgcg cgatgccctg ctcgcgcgca
      541 gcgtcgcgcc ggcgcagcgc ggactggctt tcggcgtgca ccgggccatg gacaacgccg
      601 gcgccgtcgc cgggccgctg ctcgccgcgg cgctgctcgg gctcggcgtg ccgctgcgcg
      661 agctgttcct gtggacggcg atgcccggtg cgctgacgct ctggctgatc tggcgcgtgc
      721 cggaaacgcc ggtcatcgcc gctcccgcca gcacggcgcc cgccccggct gccggcgcgc
      781 cgctgccgcc agcgctgcgc cgctatctgg cgatttacgc gctgttctcg ctcggcaacg
      841 cctcgaacct gttcctgctc tggcgcgcgc acgaactcgg gctcgccgat gccgcgctgc
      901 cgctgctgtg ggcgctggtg tcggcggtcg cgatgctggg ctcggcgccg ctgtcgggac
      961 tgtcggatcg catcggccgg cgccggctga tcgccggcgg ctggacgctg ttcgcgctgg
     1021 tctacgcgct gctcggctgg ccggggctgc cgccggcggg gctgtgg
//
LOCUS       DAOQJH010000777         1063 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_164250_length_1063_cov_4.458333, whole genome shotgun
            sequence.
ACCESSION   DAOQJH010000777 DAOQJH010000000
VERSION     DAOQJH010000777.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1063
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_164250_length_1063_cov_4.458333"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..325)
                     /locus_tag="PLQ18_14155"
     CDS             complement(<1..325)
                     /locus_tag="PLQ18_14155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018954889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNE02847.1"
                     /translation="MTAVAAKPEGLLIDLAGVLHVNDRALPGAAETLARLAAAGVPHA
                     FVTNTTRQTRTAIAAQLAGLGLPVPESRIVSAVDATHDWLAARRLRPHLLVHPAVIAA
                     FADLEQ"
     gene            491..>1063
                     /locus_tag="PLQ18_14160"
     CDS             491..>1063
                     /locus_tag="PLQ18_14160"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013234078.1"
                     /note="catalyzes the formation of L-threonine from
                     O-phospho-L-homoserine; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HNE02848.1"
                     /translation="MQYLSTRGDAAARTFSDILLAGLAPDGGLYLPEHYPQVSAAELA
                     AWRELPYAELAFEILRKFATDLPEADLRALTAKTYTAAVYRSDAITPLRTLEPGLHIL
                     GLSNGPTLAFKDMAMQLLGNLFEYVLGQSGEELNILGATSGDTGSSAEYAMRGKRGIR
                     VFMLSPHGKMSAFQTAQMFSLLDPNIHNLAV"
BASE COUNT          168 a          387 c          356 g          152 t
ORIGIN      
        1 cctgttcgag atcggcaaaa gccgcgatca cggccggatg cacaagcaga tgcggacgca
       61 ggcggcgcgc ggccagccag tcgtgcgtgg catcgacggc gctgacgatg cgcgattccg
      121 gcaccggcag gccgagcccg gcgagctggg cggcgatcgc cgtgcgcgtc tggcgcgtgg
      181 tgttggtgac gaaggcatgc ggcacgccgg ccgcggcgag gcgcgccagc gtttcggccg
      241 ctcccggcag cgcgcggtcg ttgacgtgca gcacgccggc cagatcgatc agcagcccct
      301 cgggtttcgc ggcaacagcg gtcatctgca ggctccggtc ggcgggctgc gcaccagcat
      361 aacgctttgc tgcgctgcgc aaagcccgcc ggcgactagc tgacggcttg cggcgcaccc
      421 gtgggcgcgg gcggtgcttg cgtataatcg ccgctctgtt tccgcgccca cccggggaat
      481 ccgcaccgtc atgcagtacc tcagcacgcg cggcgacgcc gcggcccgca cgttttccga
      541 catcctgctc gccggcctgg cgccggacgg cggcctctac ctgcccgagc actatccgca
      601 ggtgagcgcg gccgaactcg ccgcctggcg cgagctgccg tatgcggaac tggccttcga
      661 aatcctgcgc aagttcgcga ccgacctccc ggaagccgac ctgcgcgcac tgaccgcgaa
      721 gacctacacc gccgccgtct accgcagcga cgcgatcacg ccgctgcgca cgctcgaacc
      781 cggcctgcac atcctcggtc tctcgaacgg cccgacgctg gcgttcaagg acatggcgat
      841 gcagctgctc gggaatctct tcgagtacgt gctcggacag agcggcgagg agctgaacat
      901 cctcggcgcc accagcggcg acaccggatc gagcgccgag tacgcgatgc gcggcaagcg
      961 cggcatccgc gtgttcatgc tgtcgccgca cggcaagatg agcgccttcc agaccgcgca
     1021 gatgttcagc ctgctcgacc cgaacatcca caacctcgcc gtg
//
LOCUS       DAOQJH010000778         1059 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_165632_length_1059_cov_3.272908, whole genome shotgun
            sequence.
ACCESSION   DAOQJH010000778 DAOQJH010000000
VERSION     DAOQJH010000778.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1059
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_165632_length_1059_cov_3.272908"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            334..651
                     /locus_tag="PLQ18_14165"
     CDS             334..651
                     /locus_tag="PLQ18_14165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02849.1"
                     /translation="MLDFAAVEALLPEVAARLRAEFEDASEQSDAELHAFLRPVLRHA
                     ALHGFADADTGFVYAACAWLTGEDFASAFETPKTILAASAPVADKAAALEDWLTGLID
                     PAD"
     gene            727..>1059
                     /locus_tag="PLQ18_14170"
     CDS             727..>1059
                     /locus_tag="PLQ18_14170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015353094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNE02850.1"
                     /translation="MSDLAKIVVILAAAVLAVPLAKRLGLGAVIGYLLAGVLIGPWGL
                     ALIREHEEILHVGELGVVLLLFIVGLELQPSRLWALRRPVFGLGGLQVLLTGGAVMAV
                     MQWLGWHPG"
BASE COUNT          124 a          377 c          395 g          163 t
ORIGIN      
        1 cctcggtgat caccgtgtgc ggacgcggat cggcgtgcgc gcgcggcgcg gccagaccgc
       61 cggcaatcag cagcaccgcg agcgcggcac tgccggcgag atggacggaa cgcgtggcag
      121 gtcggttcat gatcgaagct ccggggttcg ggttcgttgt cgccgctcgg ggccccgtgt
      181 cacagcggcg tgagctgtcc gggacgggcc cggcacatcc cgacagagcc gcacgcccgg
      241 gcggaagttc cggctggcat ggccggcggt ggaccgcagc cgggcgcgcg gctaccgtgc
      301 cgcctccgtt cccgaagccg cggagatgcc gccatgctcg atttcgccgc cgtcgaggcg
      361 ctgctgcccg aggtcgccgc gcggctgcgc gccgagttcg aggacgcgag cgagcagtcc
      421 gatgccgaac tgcacgcctt cctgcgcccg gtgctgcgcc acgccgcgct gcacggcttt
      481 gccgatgccg acaccggctt cgtctacgcg gcctgcgcgt ggctgaccgg cgaggatttc
      541 gcgagcgcct tcgagacgcc gaagaccatc ctcgccgcca gcgcgccggt tgccgacaag
      601 gccgcggcgc tggaggactg gctgaccggc ctgatcgacc cggcggactg acgcggcgcg
      661 ccgtcatctg gctcaggcac actgtcgggc acgcattccc gccgtcctgc cgccgcaacc
      721 gccatcgtgt ccgatctcgc gaaaatcgtc gtcatcctcg ccgccgcggt gctggccgtg
      781 ccgctcgcga agcggctcgg gctcggggcc gtgatcggct atctgctcgc cggcgtgctg
      841 atcgggccgt gggggctggc gctgatccgc gagcacgagg aaatcctgca cgtcggcgaa
      901 ctcggcgtcg tgctgctgct gttcatcgtc ggcctcgaac tgcagccctc gcggctgtgg
      961 gcgctgcgcc ggccggtgtt cgggctcggc ggcctgcagg tgctgctgac cggcggcgcg
     1021 gtgatggcgg tgatgcagtg gctcggctgg catccgggc
//
LOCUS       DAOQJH010000779         1059 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_165653_length_1059_cov_2.972112, whole genome shotgun
            sequence.
ACCESSION   DAOQJH010000779 DAOQJH010000000
VERSION     DAOQJH010000779.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1059
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_165653_length_1059_cov_2.972112"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            54..293
                     /locus_tag="PLQ18_14175"
     CDS             54..293
                     /locus_tag="PLQ18_14175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02851.1"
                     /translation="MSLRKLYGLVLVDVKADGRFGLEFAHALGEGERMDWRDYLNEFM
                     RSRITDERIPDADTISRFLRVVGDWGDDAAKDKPS"
     gene            complement(354..>1059)
                     /locus_tag="PLQ18_14180"
     CDS             complement(354..>1059)
                     /locus_tag="PLQ18_14180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014706095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNE02852.1"
                     /translation="AEGDAIAGIIVEPVAGNMNCIPPVPGFLEALRTHCDAAGAVLIF
                     DEVMTGFRVARGGAQQHYGITPDMTTLGKVIGGGMPVGAFGGKRAIMEKLAPLGPVYQ
                     AGTLSGNPVAMAAGLATLALIEAPGFHERLSALSARLCAGLEQAAGAAGIALTTNHVG
                     GMFGFFFTDKKVSSYAEATNCDVERFKRFFHGMLAEGVYLAPSAFEAGFVSSALTDAD
                     IDATIAAAERVFGRIG"
BASE COUNT          184 a          338 c          355 g          182 t
ORIGIN      
        1 gctatttgat caaggtgttg aacgggcaga tgatttagat gcgtttgccc tggatgtcgc
       61 tgcgcaagtt atatgggctg gtactcgtgg acgtaaaagc agacggccgc ttcggtcttg
      121 aatttgctca tgctctaggc gagggagagc gtatggattg gcgcgactac ttaaatgaat
      181 tcatgcgctc ccggatcaca gacgagagga ttcccgacgc cgataccata agccgcttcc
      241 tgcgcgtcgt cggcgactgg ggggatgacg ctgcgaagga caagccgtcg tagcgtgcag
      301 aacctgatcc gggcaggaag ctctttatgg aagcgccgtg accggcggac ggctcagccg
      361 atccgcccga acacccgttc cgccgccgcg atcgtcgcgt cgatgtcggc atcggtcagt
      421 gccgacgaaa caaaccccgc ctcgaacgcc gagggcgcca gatacacgcc ttcggcgagc
      481 atgccgtgga agaagcgctt gaagcgttcc acgtcgcagt tcgtggcttc ggcgtagctc
      541 gacacctttt tgtcggtgaa gaagaacccg aacatgccgc cgacgtggtt cgtcgtcagc
      601 gcgatgccgg cggcaccggc ggcttgctcc agccccgcgc agagccgcgc cgacagcgca
      661 ctcaggcgct cgtggaagcc cggcgcctcg atcagtgcga gcgtcgcgag gcccgcggcc
      721 atcgccaccg gattgcccga cagcgtgccg gcctgataga ccgggccgag cggggcgagc
      781 ttttccatga tcgcgcgctt gccgccgaag gccccgaccg gcatgccgcc gccgatgacc
      841 ttgccgagcg tggtcatgtc cggcgtgatg ccgtagtgct gctgcgcgcc gcccctggcg
      901 acgcgaaagc cggtcatgac ctcgtcgaag atcagcacgg cgccggcggc gtcgcagtgc
      961 gtgcgcaggg cttcgaggaa gcccggcacc ggcgggatgc agttcatgtt gccggcgacc
     1021 ggctcgacga tgatgcccgc gatcgcgtcg ccctccgcg
//
LOCUS       DAOQJH010000780         1058 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_165957_length_1058_cov_4.237288, whole genome shotgun
            sequence.
ACCESSION   DAOQJH010000780 DAOQJH010000000
VERSION     DAOQJH010000780.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1058
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_165957_length_1058_cov_4.237288"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(3..722)
                     /locus_tag="PLQ18_14185"
     CDS             complement(3..722)
                     /locus_tag="PLQ18_14185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HNE02853.1"
                     /translation="MSHNHSGHHDAGTQPCAICGKPFARRDLVPAALVREPLQQLLQR
                     ERPDWNAQAWICRPDLAELRARYVHSVLEAEKGELTGLEQAVLDSLRRHEILSANVDA
                     EQEQAWSFGERLADRIASFGGSWSFLILFMLFIAVWIAMNSLVMFFRPLDPFPYILLN
                     LLLSCLAAVQAPIIMMSQNRQEARDRVRAEHDYQVNLKAELEIRHLHEKIDHLLNHQW
                     ERLLQIQEVQVDLLTELRRPR"
BASE COUNT          163 a          363 c          351 g          181 t
ORIGIN      
        1 ttttaccgcg gccggcgcag ctcggtcagc agatcgacct gcacctcctg gatctgcagc
       61 agccgctccc actggtggtt cagcaggtgg tcgatcttct cgtgcaggtg gcggatctcc
      121 agctcggcct tcaggttgac ctgatagtcg tgctcggcgc gcacgcggtc gcgggcctcc
      181 tgacggttct ggctcatcat gatgatcggc gcctgcacgg ctgcgaggca cgacagcagc
      241 aggttcagca ggatgtacgg gaacgggtcg agcggccgga agaacatcac cagcgagttc
      301 atcgcgatcc agacagcgat gaacagcatg aacaggatca ggaagctcca gctgccgccg
      361 aagctcgcga tgcgatcggc gagccgctcg ccgaagctcc aggcctgctc ctgctcggca
      421 tcgacgttgg ccgacaggat ctcgtgccgg cgcaggctgt cgagcaccgc ctgctccagc
      481 ccggtcagct cgcccttctc ggcttcgagc accgaatgca cgtagcgcgc gcgcagctcg
      541 gccagatcgg gccggcagat ccaggcctgc gcgttccagt ccggccgctc gcgctgcagc
      601 agctgctgca gcggctcgcg caccagcgcc gccggcacca gatcgcgccg cgcgaacggc
      661 ttgccgcaga tcgcgcaggg ctgcgtgccc gcgtcgtgat ggcctgagtg gttgtgggac
      721 atcggcagcc ttcctttcgg gtcgaatccg gggcggaggc cggcagcgta cgccgccggc
      781 attgcgcccg cctgcaagcc atgcagggat gcatcggcgc gacacgcatc cgtgcgcatc
      841 ctgccgggcg cagcgcgccg tagcggccag cgatggggat ttcgcggcag aaaccctgta
      901 acggctgcga aaaatacttt gccgggcacg gaactttttc gtgtaaccgc cggaaccggc
      961 gcccggccat gcgagtccgt atgcccgccg tcgtcccgtc cgtggagtcc atgccgatgt
     1021 tccgacgtct gtcctgctgc ttcgcgctgc tgctcgct
//
LOCUS       DAOQJH010000781         1053 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_167690_length_1053_cov_3.683367, whole genome shotgun
            sequence.
ACCESSION   DAOQJH010000781 DAOQJH010000000
VERSION     DAOQJH010000781.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1053
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_167690_length_1053_cov_3.683367"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1053)
                     /gene="cas3u"
                     /locus_tag="PLQ18_14190"
     CDS             complement(<1..>1053)
                     /gene="cas3u"
                     /locus_tag="PLQ18_14190"
                     /inference="COORDINATES: protein motif:HMM:TIGR02621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-U CRISPR-associated helicase/endonuclease
                     Cas3"
                     /protein_id="HNE02854.1"
                     /translation="LGAAETDPAAAPVLHAVAARLRVLAGTTAGSPAPVLAVERLRGG
                     VPRENAWAGSPCQPLLLLSTVDQLGSRLLFRGYGLSEFAWPLHAGLLGEDTLILLDEA
                     HLSQPFHDTVRAIRRYCGADWRETPPLRPVAIVPLSATQGAAEAGDALRLTPADRAHP
                     ELARRLRAAKPAELLACRAEVFIDRLADSARRLLAACPTTTAPHSPGDTADLFAAPAP
                     AAPVVGIVVNRIATARALYAELRATGDADALLLIGRMRPLDRDRLVEAYLPRIRAGRA
                     AGDNPRPLYVVATQTIEVGADLDFDALVTESAPLDALRQRFGRLNRLGARSRAPAAIL
                     HVSDAGKDDPVYGGAIA"
BASE COUNT          137 a          367 c          427 g          122 t
ORIGIN      
        1 agcgatggcg ccgccgtaga ccggatcgtc cttgccggca tcgctgacgt gcaggatcgc
       61 cgccggtgcg cggctgcggg cgccgagccg gttcagccgg ccgaaacgct ggcgcagcgc
      121 atcgagcggc gcggattcgg tgacgagcgc atcgaagtcg agatcggcgc cgacttcgat
      181 ggtctgcgtc gcgaccacgt acagcgggcg cggattgtcg ccggcagcgc gcccggcgcg
      241 gatgcgcggc agatatgcct cgaccagccg gtcgcgatcg agcggacgca tgcggccgat
      301 cagcagcagc gcgtcggcat cgccggtggc gcgcagctcc gcgtacagcg cccgcgcggt
      361 ggcgatgcgg ttgacgacga tgccgaccac cggcgcggcc ggtgccggag cggcgaacag
      421 gtcggcggtg tcgcccggtg agtgcggtgc cgtcgtcgtc ggacaggcgg ccagcagccg
      481 gcgggcgctg tcggcgagcc ggtcgatgaa gacctcggcc ctgcaggcca gcagttcggc
      541 cggcttggcg gcacgcaggc gccgcgccag ttccggatgc gcgcgatcgg ccggcgtcag
      601 gcgcagcgca tcgccggctt cggctgcgcc ctgcgtggcg ctcagcggca cgatcgcgac
      661 cggccgcagc ggcggcgtct cgcgccagtc ggccccgcag tagcggcgga tggcgcgcac
      721 ggtgtcgtgg aagggctgcg acaggtgcgc ttcgtcgagc aggatcagcg tgtcctcgcc
      781 gagcaggccg gcgtgcagcg gccaggcgaa ctcgctgagg ccgtagccgc ggaacagcag
      841 ccgcgagccg agctgatcga cggtcgagag cagcagcagc ggctggcacg gcgatccggc
      901 ccaggcgttc tcgcgcggca cgccgccgcg cagccgctcg accgcgagca ccggcgccgg
      961 gctgccggct gtggtgccgg ccagtacccg cagccgggcg gcaacggcgt gcagcaccgg
     1021 ggcggcggcg gggtcggtct cggcggcgcc gag
//
LOCUS       DAOQJH010000782         1033 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_174769_length_1033_cov_4.699387, whole genome shotgun
            sequence.
ACCESSION   DAOQJH010000782 DAOQJH010000000
VERSION     DAOQJH010000782.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1033
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_174769_length_1033_cov_4.699387"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..322)
                     /locus_tag="PLQ18_14195"
     CDS             complement(<1..322)
                     /locus_tag="PLQ18_14195"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013803964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046917 - triphosphoribosyl-dephospho-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase"
                     /protein_id="HNE02855.1"
                     /translation="MPIALHPLDTPATADLGRAAVRALYEELALAPKPGLVSPLDSGS
                     HSDMTVQTFLRSLFALRSYFRDIAAAGAEGADFPALNALGRHAETRMLAATGGINTHK
                     GAVFM"
     gene            complement(536..>1033)
                     /gene="mdcG"
                     /locus_tag="PLQ18_14200"
     CDS             complement(536..>1033)
                     /gene="mdcG"
                     /locus_tag="PLQ18_14200"
                     /inference="COORDINATES: protein motif:HMM:TIGR03135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051191 - prosthetic group biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase
                     holo-[acyl-carrier-protein] synthase"
                     /protein_id="HNE02856.1"
                     /translation="ADTVRLGLTRLGRRRLGVALGHAALTRIAPPLALTEVLDSAPVS
                     WRARARAVLDLAGFCGAGVQVHVYGSLSWQHLSGTAYVTPDSDLDLLLCIDGSGRSNG
                     DILSLLAGLTALEGGTPRLDGELRLPDGSDVAWRELAARPPKLLVRSRSTLGFRPLAE
                     LLAPA"
BASE COUNT          141 a          356 c          403 g          133 t
ORIGIN      
        1 gcatgaacac cgcacccttg tgcgtgttga tgccgccggt cgccgccagc atgcgggttt
       61 ccgcgtgccg gccgagtgcg ttcagcgccg gaaaatccgc gccttcggcg ccggcggcgg
      121 cgatgtcgcg gaagtagctg cgcagcgcga acaggctgcg caggaaggtc tgcaccgtca
      181 tgtcgctgtg gctgccggaa tccagcgggc tgaccagccc gggcttcggc gccagcgcca
      241 gctcctcgta cagcgcgcgc accgcggcgc ggccgagatc ggcggtggcc ggggtgtcga
      301 gcggatgcag cgcgatgggc agggcggcgg tttgtcggag cacggggcag acctctcgga
      361 ggggaaggag caggatcggg ccggtccatg acaggcgtgt cagcgtcgca cgccgatccg
      421 cggcggtgcc ggctgccccc gccggatttt cctgcgcgcg agggggagga aactgacggg
      481 cgaggcgcct tccgtggcgc ccgctccggc atgtgatgca ggttgcggac cggtttcagg
      541 ccggtgccag cagttcggcg agcggccgga agccgagcgt gctgcggctt cgcaccagca
      601 gcttcggcgg ccgggccgcg agttcgcgcc aggcgacatc gctgccgtcg ggcaggcgca
      661 gttcgccgtc gagccgcggc gtgccgcctt cgagcgccgt caggccggcg agcagcgaaa
      721 ggatgtcgcc gttgctcctg ccgctgccat cgatgcacag cagcagatcg aggtcggagt
      781 ccggcgtcac gtacgccgtg cccgacaggt gctgccagct cagcgagccg tagacgtgca
      841 cctgcacgcc cgcaccgcag aagccggcca gatcgagcac ggcccgcgcc cgcgcccgcc
      901 aggacaccgg cgcgctgtcc agcacttcgg tgagcgcgag cggcggggcg atgcgcgtca
      961 gggccgcgtg cccgagcgcg acgccgagcc ggcggcggcc gagccgcgtc aggccgaggc
     1021 gcacggtgtc ggc
//
LOCUS       DAOQJH010000783         1031 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_175526_length_1031_cov_4.157787, whole genome shotgun
            sequence.
ACCESSION   DAOQJH010000783 DAOQJH010000000
VERSION     DAOQJH010000783.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1031
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_175526_length_1031_cov_4.157787"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..270)
                     /locus_tag="PLQ18_14205"
     CDS             complement(<1..270)
                     /locus_tag="PLQ18_14205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006635637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HNE02857.1"
                     /translation="MSRRAASGPAGGARCAFPVVGLGASAGGVEAFNEFLSAMPAASG
                     MAFVFVLHLDPERSSVLTEILARHTTMPVTEVTHPVQVVPDHVYVI"
     gene            756..>1031
                     /locus_tag="PLQ18_14210"
     CDS             756..>1031
                     /locus_tag="PLQ18_14210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02858.1"
                     /translation="MLPAATDYAILGFDPHLHCCYASGSALGTLGIENPLRGTELQAL
                     APALRTVVSGLCRCVFAGALTETGITLGPQRLRLTGMPLLDSSGAISM"
BASE COUNT          181 a          363 c          318 g          169 t
ORIGIN      
        1 gatcacgtag acgtgatcgg gcaccacctg caccggatgc gtgacctccg tgaccggcat
       61 ggtcgtgtgc cgcgccagga tttcggtcag cacgctgctg cgctcgggat cgagatgcag
      121 cacgaacacg aaggccatgc cgctggcggc cggcatcgcg ctcaggaatt cgttgaacgc
      181 ctcgacgccc cctgccgaag cgccgaggcc gaccaccgga aatgcacacc gggccccgcc
      241 ggccggccct gatgcagctc gtctggacat cgtcttcccc cctctgcgcc acgaatccgc
      301 cggcaagttt aagtccgctt cgcccgtcct cctcggcttc tcccgacgaa gtaggtacaa
      361 accggtagcc gccgtggaaa gcgccaatgg cgatgcactg ctcctgacag catagtgaca
      421 ggcagccctg catcccgagc cacggccagg atggtggcag gcagcgagac ggggccaaga
      481 gaagcgacag catgatcctg gggacacgcg ccagcgtgtg cgccagaagc agcgcggcgg
      541 ttcaggacga gcctccgcgc cgctttttca tgctttttcc attaatgacc gccacttgca
      601 aggccatcgt acagacagga cctgcccggg agcagcgacg gcatgccgga tcgaaaccgg
      661 gtggagggag aaaagtctgc catgtccgaa cctctgcccg gcctcgatgc cccctgtcag
      721 gaacacttca gggctttctc gattccctct gtccgttgct gcccgcagcg accgactacg
      781 ccatcctcgg cttcgatcct cacctgcact gctgctacgc cagcggctct gcactcggta
      841 cgctcggcat cgaaaatccg ctccggggca cggagttgca ggcgctcgcg ccggcgctgc
      901 gcacggtcgt gagcgggctt tgccgctgcg tcttcgccgg cgcgctgacc gaaaccggga
      961 tcacgctcgg cccgcagcgg ctgcgcctga ccggcatgcc gctgctcgac agcagcggtg
     1021 cgatcagcat g
//
LOCUS       DAOQJH010000784         1030 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_175922_length_1030_cov_3.146667, whole genome shotgun
            sequence.
ACCESSION   DAOQJH010000784 DAOQJH010000000
VERSION     DAOQJH010000784.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1030
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_175922_length_1030_cov_3.146667"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..274)
                     /locus_tag="PLQ18_14215"
     CDS             complement(<1..274)
                     /locus_tag="PLQ18_14215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02859.1"
                     /translation="MAGNRRPDTGRWDTALDPLCVPLFLSLQWARSRSLAHMQPVLSR
                     HRLSAAEFDVLATLRNSAPPHRLTPSQVRDQVVITSGGLTKLMLQLE"
     gene            586..675
                     /locus_tag="PLQ18_14220"
                     /pseudo
     CDS             586..675
                     /locus_tag="PLQ18_14220"
                     /inference="COORDINATES: protein motif:HMM:NF013918.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(817..1030)
                     /locus_tag="PLQ18_14225"
                     /pseudo
     CDS             complement(817..1030)
                     /locus_tag="PLQ18_14225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167713.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          161 a          344 c          341 g          184 t
ORIGIN      
        1 cttcgagctg cagcatcagc ttggtcagcc cgcccgaagt gatgacgacc tgatcgcgta
       61 cctgcgacgg cgtcagccgg tggggcgggg cgctgttgcg cagcgtcgcc agcacgtcga
      121 actcggccgc cgacagccgg tgccggctca gcaccggctg catgtgcgcc agcgaacggc
      181 tgcgtgccca ttgcagcgac aggaacagcg gcacgcacag cgggtcgagc gcggtatccc
      241 agcgcccggt gtccggacga cggtttccgg ccatgcctgc agttcctccg gtggtggtgg
      301 ggattgcaac gcaatcgcgg gtcttgcgta acgcagggta acttatcttg ctggaaagat
      361 attggctgcc tagcattccg gtgcatccct catgcagacc agcgttcagg cggcgccatc
      421 atgcgtgtca tccagcgggt tttcctggca tttccggtat tgctcacggc gctctggatt
      481 ctgggcgatc ccggcgcctt cagcggactg aacggattct ttgcctggcg caatgtcctg
      541 atgcagtaca gcggtgtgct cggcatcggc gtcatgagtt tcgcgatgct gctgagtctg
      601 cgtccggcct ggttcgaacg ccggctcggc ggactcgaca agctgtaccg gctgcaccga
      661 tggctcggca tcgccggact gtcgatcacg gttgcgcact ggctgctggc gcagggaccg
      721 aaatggctgg tgcagctcgg ctggctgcag cgcggcaacc ggccgccccg gcccctgctg
      781 cccgaaggct cgctgcagca atggttcgcg catcagcgcg gattcgccga aggcgtcgat
      841 ccccaccacg gcgccttcca ggccaacgta ctttcgccac caatccgaca ccccggcttc
      901 caccgccacg cgcggcacgc cgctgggcag cacgctgtcg cgataggcgg cgtcctggcg
      961 gtcgaagacg aagctcgacg gcatcgaaac gacgcggcgc atccggcgca tcgtgggacc
     1021 ggacgtcacc
//
LOCUS       DAOQJH010000785         1015 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_181592_length_1015_cov_1.982292, whole genome shotgun
            sequence.
ACCESSION   DAOQJH010000785 DAOQJH010000000
VERSION     DAOQJH010000785.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1015
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_181592_length_1015_cov_1.982292"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(66..815)
                     /gene="trmD"
                     /locus_tag="PLQ18_14230"
     CDS             complement(66..815)
                     /gene="trmD"
                     /locus_tag="PLQ18_14230"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HNE02860.1"
                     /translation="MRFDVVTLFPDLFGPHLTQGVTRRAFESGQVQVRLWPLRDFADD
                     GYRRVDERPFGGGPGMVMLAEPLLAALDAVFAERGETAPVVHFTPTGRRLDQAWVREF
                     AAGAGAVLICGRYEGVDQRFVERRVTHEVSLGDFVLSGGELPALALLDAVARLQPGVL
                     NAPSHEQDSFSDGLLEGPRFTRPEQLLVDGQTLAVPPVLLSGNHGEIARWQRERSLER
                     TARRRPDLIVAARASGRLSAEDERFLDKLDL"
     gene            complement(841..>1015)
                     /locus_tag="PLQ18_14235"
     CDS             complement(841..>1015)
                     /locus_tag="PLQ18_14235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="16S rRNA processing protein RimM"
                     /protein_id="HNE02861.1"
                     /translation="ECLGTVSGLLDTGAHGVLCVAGPGAADVERLIPFVAAYVDRVDL
                     PGRVIHVDWQRDY"
BASE COUNT          178 a          365 c          339 g          133 t
ORIGIN      
        1 atggatgatt gtcggcgacc gcaacgtgcg gctgtatgtc cggcagagga tcgaaaagct
       61 cttgattata ggtcgagctt gtccaggaat cgctcgtcct cggccgagag ccgccccgac
      121 gcccgcgccg ccacgatgag atcgggccgc cggcgagccg tccgctccag cgaacgctca
      181 cgctgccagc gcgcgatctc gccgtggttg ccggacagca gcacgggcgg gacggccagc
      241 gtctgcccgt cgaccagcag ttgctcgggc cgtgtgaagc gcggaccttc gagcagtccg
      301 tccgaaaaac tgtcctgctc gtgcgacggc gcattcagca cccccggctg cagacgcgcc
      361 acggcatcga gcaaggcgag cgccgggagt tcgccgcccg agagcacgaa gtcgcccagg
      421 ctcacctcgt gcgtgacgcg gcgctcgacg aagcgctgat ccaccccttc gtagcgaccg
      481 cagatcagca ccgccccggc gccggcagcg aattcgcgca cccatgcttg atcgagccgc
      541 cgaccggtcg gcgtgaagtg cacgaccggc gccgtctcgc cacgctcggc gaagactgcg
      601 tcgagcgcgg cgagcagcgg ctcggcgagc atcaccatgc cggggccgcc gccgaacggc
      661 cgttcgtcga cgcgcctgta cccgtcgtcg gcgaagtcgc gcagcggcca caggcgtacc
      721 tgcacctggc ccgactcgaa cgcgcggcgc gtgacgccct gcgtgaggtg cggcccgaac
      781 agatccggaa acagcgtgac gacgtcgaag cgcatggcga cgcgcgatgc cggtgacgaa
      841 tcagtagtcg cgctgccagt cgacgtggat cacgcgaccc ggcagatcga cgcggtcgac
      901 ataagcggcg acaaacggta tcagccgctc cacgtcggcg gccccgggcc cggcaacgca
      961 cagcacaccg tgcgcaccgg tgtctaggag cccgctcacg gttcccaagc attca
//
LOCUS       DAOQJH010000786         1009 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_183691_length_1009_cov_4.192872, whole genome shotgun
            sequence.
ACCESSION   DAOQJH010000786 DAOQJH010000000
VERSION     DAOQJH010000786.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1009
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_183691_length_1009_cov_4.192872"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..381)
                     /locus_tag="PLQ18_14240"
     CDS             complement(<1..381)
                     /locus_tag="PLQ18_14240"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE02862.1"
                     /translation="MSHPNLAMDGARILVVDDAPDAIRLIDGLLSPHYRLHVACNGES
                     ALRILAGPLPIDLILLDVVMPDLDGYEVCRRLRALPGREDVPVVFLTGNRDPASETAG
                     FAAGATDFVSKPVSAPVLLARVRTH"
     gene            complement(451..>1009)
                     /locus_tag="PLQ18_14245"
     CDS             complement(451..>1009)
                     /locus_tag="PLQ18_14245"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HNE02863.1"
                     /translation="QHTGGKLDLYETLLAQFARDHLDADRRVRRLLAGSERLAAVRLA
                     HTLKGSAGTLGAVRLQFRAAALESVLCRPDKPADEALADFAEAFAELRAGIEQWQAER
                     QPVLDAVGPGAPRRDPAELAQQLALLIGDHDTSALAVAEQLVAACAGASLEAQVREMT
                     QAVRNYDFTRAAALLRGSGLLGSRS"
BASE COUNT          136 a          338 c          401 g          134 t
ORIGIN      
        1 gtgcgtgcgc acgcgcgcga gcagcaccgg cgcgctgacc ggcttgctga cgaaatcggt
       61 ggcgccggcc gcgaagccgg cggtttccga ggccgggtcg cggttgccgg tcaggaacac
      121 caccggcacg tcctcgcgtc ccggcagcgc gcgcagccgg cggcagacct cgtagccgtc
      181 gaggtcgggc atgaccacgt cgagcaggat caggtcgatc ggcagcggtc cggcgaggat
      241 gcgcagcgca ctttcgccgt tgcaggcgac gtgcagccgg tagtgggggc tcagcaggcc
      301 gtcgatcagg cggatggcat ccggcgcgtc atcgacgacg aggatgcgtg ctccgtccat
      361 ggcaaggttg gggtgagaca cggcaggtct ccggtccgga ccggcggcat ggcgtccctg
      421 gccgccggtc ctcagggggg aaggtccgga tcagctgcgc gagcctagca ggcccgagcc
      481 gcgcagcaag gcggcggcgc gcgtgaagtc gtagttgcgc accgcctgcg tcatctcgcg
      541 gacctgcgcc tccagcgagg cgccggcaca ggcggcgacc agctgctcgg cgacggcaag
      601 cgcggaggtg tcgtgatcgc cgatcagcag cgcgagctgc tgcgcgagtt cggccgggtc
      661 gcggcgcggg gcgcccgggc ccacggcatc gagcaccggc tggcgctcgg cctgccattg
      721 ctcgatgccg gcgcgcagtt cggcgaatgc ctcggcgaaa tcggcgagcg cctcgtcggc
      781 cggcttgtcc gggcggcaca gcacgctttc gagcgccgcg gcgcgaaact gcaggcgcac
      841 ggcgccgagc gtgccggcgc tgcccttgag cgtgtgtgcg aggcgcaccg cggccagccg
      901 ttcgctgccg gcgagcaggc ggcgcacgcg ccggtcggca tcgagatggt cgcgggcgaa
      961 ctgcgcgagc agggtttcgt agagatcgag cttgccgccg gtgtgctgg
//
LOCUS       DAOQJH010000787         1009 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_183903_length_1009_cov_2.046122, whole genome shotgun
            sequence.
ACCESSION   DAOQJH010000787 DAOQJH010000000
VERSION     DAOQJH010000787.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1009
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_183903_length_1009_cov_2.046122"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(144..671)
                     /locus_tag="PLQ18_14250"
     CDS             complement(144..671)
                     /locus_tag="PLQ18_14250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005738168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HNE02864.1"
                     /translation="MAVRDALAIDLLAICEIYTPYVLYGAQTFEEVPPSLAAMTERRA
                     QVLAHGLPFLVAERGARVLGYCYATPYRSRPAYRYTVEDSIYVAHDCTAGGVGTALLE
                     ALIRRCERGPWRQMISVIGDSDNAASIRLHTRHGFEPIGICRAVGFKHGRWIDTVLMQ
                     RALGAGDRSLPSGRA"
BASE COUNT          167 a          326 c          364 g          152 t
ORIGIN      
        1 acatccgtca gggaacggga tcggtcaggc gcagcagcgg cgccggccgg ggcagccggc
       61 acggtgatgg tcgcgagcgg cgtgccgagt tcgcagtcta gccgggcagt accgcgcccg
      121 gcatgcggca ggatgtcgcg gcttcaggcc cggcccgatg gcaggctgcg gtcgccggcc
      181 ccgagtgcgc gctgcatcag caccgtgtcg atccagcgcc cgtgcttgaa gccgaccgcc
      241 cggcagatgc cgatcggctc gaagccgtgg cgggtgtgca gccggatcga cgcggcgttg
      301 tcgctgtcgc cgatcaccga aatcatctgc cgccacggcc cgcgctcgca gcgccggatc
      361 agggcttcga gcagcgccgt gccgacgccg ccggcggtgc agtcgtgggc gacgtagatc
      421 gagtcttcca cggtgtagcg gtaggccggc cgtgaccggt acggcgtggc atagcagtag
      481 ccgagcacgc gtgccccgcg ttcagcgacc aggaacggca ggccgtgcgc cagcacctgc
      541 gcacggcgct cggtcatcgc cgccagcgac ggcggcactt cctcgaaggt ctgggcgccg
      601 tagagcacgt agggcgtgta tatctcgcag atcgcgagca ggtcgatcgc gagcgcatcg
      661 cgcacggcca gcgcggtgcc cgcggcacgg gggtcagggg ctgaagtttc gaaatcctgc
      721 ccggtttcag ccggccgggg aggcagggcg gaatcggtac tcatcggcgg ggcggcatga
      781 ctggctgcga tgaatcggca gcactccgcc ggcctgtgca gccgtgacgg acacctgtcg
      841 gcctgtgccg gcccgcagaa aatcttttgc tgcgggccgg acggactcac ggtgtcatga
      901 tcattaccgg attttcatga tctgcatcaa caaatccggc tgatgcgcgg gcaagaaaaa
      961 ctgattcgtc aacatcgcag gacagatcat cgcgaatggc ccggatacg
//
LOCUS       DAOQJH010000788         1003 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_186168_length_1003_cov_3.039030, whole genome shotgun
            sequence.
ACCESSION   DAOQJH010000788 DAOQJH010000000
VERSION     DAOQJH010000788.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1003
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_186168_length_1003_cov_3.039030"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..637)
                     /locus_tag="PLQ18_14255"
     CDS             complement(<1..637)
                     /locus_tag="PLQ18_14255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HNE02865.1"
                     /translation="MSFTVTIQPSGHTLSTEAGESILEAALRQGLTLPYGCRDGACGT
                     CRGKVLSGEVDHGKAQDHALSQADRDQGLALFCCASPRSDLVIESREVRKAGDIPVKT
                     LPVRVQSLERAAPDVMIVKLKLPASERLQFLAGQYVDILLKDGRRRSFSLANAPHDDA
                     LLELHIRQVPGGLFTEQVFSQMKEKDILRINGPHGTFFLREDSPKPIVMIAG"
     gene            618..>1003
                     /locus_tag="PLQ18_14260"
     CDS             618..>1003
                     /locus_tag="PLQ18_14260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02866.1"
                     /translation="MVTVKLMVPLRFLPLRGVGTLRAPDCYNSPVKRLLIVGCGDVAW
                     RALPRLRNRYRVFSLLRDPLQAPRWRDAGAVPLIADLDHPASLHRIRGLAEDVLHLAP
                     PPDRSGRDTRTQALLAALERGKSLPQR"
BASE COUNT          162 a          345 c          326 g          170 t
ORIGIN      
        1 ccccggcgat catcacgatc ggcttcggcg agtcctcgcg caggaagaag gtgccatgcg
       61 gaccgttgat ccgcaggatg tccttctcct tcatctgcga gaacacctgc tccgtgaaca
      121 ggccgccggg aacctgacgg atatgcagct cgagcagcgc gtcgtcgtgc ggcgcattgg
      181 cgagcgaaaa gctgcgccgg cggccgtcct tgagcaggat atcgacgtac tgtccggcga
      241 gaaactgcag gcgttcgctg gcgggcaact tcagcttgac gatcatgacg tcgggagcgg
      301 cacgttcgag cgactgcacc cgtaccggca gcgtcttgac cggaatgtcg ccggccttgc
      361 gcacttcccg cgattcgatg accaggtcgg accgcggcga ggcgcagcag aacagcgcca
      421 gcccctgatc acggtcggcc tgcgacagtg cgtgatcctg cgccttgccg tgatcgacct
      481 cgccgctcag gaccttgcca cgacaggtgc cgcaggcgcc atcgcggcaa ccgtagggca
      541 gcgtcaagcc ctggcgcaag gccgcttcga gaatggattc gccggcctcg gtgctcagcg
      601 tgtggcccga tggctggatg gtgacggtga agctcatggt tccccttcgt ttcttgccct
      661 tgcgcggcgt cggcacgctc cgggcgccgg attgctacaa ttcgccggtg aaaagacttc
      721 tgatcgtcgg ctgcggcgac gtagcctggc gggccctgcc ccgactgcgc aaccgctacc
      781 gcgtattctc cctgctgcgt gatccgctgc aggcgccgcg ctggcgcgac gccggggcgg
      841 tgccgctcat cgccgacctc gaccatcctg ccagcctgca tcgaatccgc ggcctcgccg
      901 aagacgtcct gcatctggca ccgcctcccg accggagcgg ccgggatacc cgcacgcagg
      961 cgcttctggc ggcactcgag cgaggaaaaa gtctaccaca gcg
//
LOCUS       DAOQJH010000789         1001 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Plasticicumulans sp. isolate AMR_MDS_0605
            NODE_186926_length_1001_cov_3.932347, whole genome shotgun
            sequence.
ACCESSION   DAOQJH010000789 DAOQJH010000000
VERSION     DAOQJH010000789.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810237
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Plasticicumulans sp. (wastewater metagenome)
  ORGANISM  Plasticicumulans sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Candidatus
            Competibacteraceae; Plasticicumulans.
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:23:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,853
            CDSs (total)                      :: 2,830
            Genes (coding)                    :: 2,812
            CDSs (with protein)               :: 2,812
            Genes (RNA)                       :: 23
            tRNAs                             :: 20
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 18 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1001
                     /organism="Plasticicumulans sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_186926_length_1001_cov_3.932347"
                     /isolate="AMR_MDS_0605"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2307179"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1001
                     /locus_tag="PLQ18_14265"
     CDS             <1..>1001
                     /locus_tag="PLQ18_14265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family peptidase"
                     /protein_id="HNE02867.1"
                     /translation="DGQPLPPVADPDAAAAGAAADTVYRWRRLDGARIARMRYSGRLD
                     PLPAAADARSVLDARSPVADPAGSWLPAASRWFPARDDEPFLYRLRLSLPGTQRGLVP
                     GELLSAVSADGRWQAEFVAELPLEGLDLFAGPYQIEERRLARAEGGEPLLIRSWFTGA
                     VEGLGAGYREAAAGYLDGYAQRFGAYPLSGYSIVSSPLPAGFAVPGITYLGESVLRLP
                     FIRTTSLRHEVLHAWWGNGVRPDYARGNWCEGLVTLIADHDGRDEETPQSALALRIDW
                     LRDLAALGPEADYPLRAFTSRRHGADQIVGYRKGAYVFLMLRERIGAAAFDAALREL"
BASE COUNT          110 a          376 c          366 g          149 t
ORIGIN      
        1 gacggccagc cgctgccgcc ggtcgccgac cccgacgcgg ccgccgccgg cgcagcggcc
       61 gataccgtgt accgctggcg ccggctcgac ggcgcgcgca tcgcgcgcat gcgttacagc
      121 ggccggctcg atccgctgcc ggccgccgcc gatgcccgca gcgtgctcga tgcccggtcg
      181 ccggtggccg atccggccgg cagctggctg ccggcggcga gccgctggtt cccggcccgc
      241 gacgacgaac ccttcctgta ccggctgcgg ctgtcgctgc ccggcaccca gcgcggcctg
      301 gtgccgggcg agctgctgag cgccgtgtcg gcggacggcc gctggcaggc cgagttcgtc
      361 gccgagctgc cgctggaagg actggatctg ttcgccggtc cctaccagat cgaggaacgc
      421 cggctggcgc gcgccgaggg cggcgagccg ctgctgatcc gcagctggtt cacgggcgcg
      481 gtcgaagggc tgggcgccgg ctatcgcgag gcggcggccg gttacctcga cggctatgcg
      541 cagcgcttcg gcgcctatcc gctgtccggc tattcgatcg tgtcctcgcc gctgccggcc
      601 ggcttcgcgg tgccgggcat cacgtatctc ggcgaatcgg tgctgcgcct gccgttcatc
      661 cgcaccacct cgctgcgcca cgaggtcctg catgcgtggt ggggcaacgg cgtgcgtccc
      721 gactacgcgc gcggcaactg gtgcgagggg ctggtgacgc tgatcgccga tcacgacggc
      781 cgtgacgagg aaaccccgca gagcgcgctg gcgctgcgca tcgactggct gcgtgatctc
      841 gccgcgctcg gccccgaggc cgattacccg ctgcgtgcct tcaccagccg ccgccacggc
      901 gccgaccaga tcgtcggcta ccgcaagggc gcctacgtgt tcctgatgct gcgcgagcgc
      961 atcggtgccg ccgccttcga tgccgcgctg cgcgagctgt g
//
